LOCUS       DAOQVQ010000001        30770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_1734_length_30770_cov_3.679407, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000001 DAOQVQ010000000
VERSION     DAOQVQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 30770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30770
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_30770_cov_3.679407"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKN22_00005"
     CDS             <1..311
                     /locus_tag="PKN22_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41110.1"
                     /translation="YSDTAVIEYHACDIEAPNVISLSSQSGNNKWFVNADGIAEFNCV
                     IVNRWGNVIYEYSDLTGSWDGRDRSGHLVPEGVYFYTIKAKIYGGDKLEKHGFIQVVH
                     "
     gene            402..1394
                     /gene="gap"
                     /locus_tag="PKN22_00010"
     CDS             402..1394
                     /gene="gap"
                     /locus_tag="PKN22_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNS41111.1"
                     /translation="MKTIGINGFGRIGRYFTKMALQQNDFSVALVNDLADIEILAHLF
                     KYDSVHGRNPFQFKVVENAFVFENGKKIVFSQEKNPENIPWKQYNVDIVVEATGLFKS
                     KELASGHIKAGAKKVIISAVPSDAAIKMVTMGINQSILDGSETIISNASCTTNNIAPL
                     IAVMKTICEIENAFITTVHSYTADQRLHDAPHKDLRRARAAGESIIPTTTGAAKAITK
                     IFPEYEGKMGGGAVRVPVKDGSLSDITMTFKGDLTIEKINQAMKAAADGYLKGILEYT
                     EDPIVSVDVLGNPHSCVYDAGLTSVVGNMVKIVGWYDNEAGYSNRLVDLINYLK"
     gene            1399..1854
                     /gene="coaD"
                     /locus_tag="PKN22_00015"
     CDS             1399..1854
                     /gene="coaD"
                     /locus_tag="PKN22_00015"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNS41112.1"
                     /translation="MKRGLFPGSFDPFTKGHEVVVRKALKLFDEVVIGVGINSTKNYM
                     FDTEKRIKHIQSLFADAPNVRIEAYNKLTVEFCKDMDAQFIIRGLRDSKDYEYERSIA
                     HMNQSISDIETVIFLTDPEFSAISSTIIREIHRNGASIEQFVTNSHLLV"
     gene            1897..3321
                     /locus_tag="PKN22_00020"
     CDS             1897..3321
                     /locus_tag="PKN22_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNS41113.1"
                     /translation="MKRLFLSFFLLLISLSVFGQTNLVTIKGYAPAFVGKKIQVEEIE
                     DYISQKTATIASSTVESDSTFSMSFNIAETQRVLVKIENNRSFMYVQPKGQYSILFPQ
                     KDPRQPFRPAGNQVELTFFDLDSTDINYRILGFNRWMDNFLALNYADAYKNPIRFNHK
                     VDSFKTVVENYYVQDTGKYIFDYVRFTIANTIDNIQQAGNRNRYEKHDFYIKHQKVLY
                     HNDAYMDYFLKFYSSMMSIIPMEVNNRVYLGLLKSSPSLIMNALGLEYTLINRRIREM
                     VMIKMLSELYYSPDYPQTNIITVLDSVKNHALFSANKIIAANIINRLTEVVNGGKAPD
                     FVVKNQKGDLKGLSDYKGKYLYVHFYNPMDENNRKEIPLLQNLYEKYSSEITFITICK
                     ESDVANSKEDKTLKNIKWDVAIVPNENSIFSYYKITTFPQYVLIDPYSYIVQAPALGP
                     QPNSTYKTIEKVFFDIQRAIKQKD"
     gene            complement(3318..3626)
                     /locus_tag="PKN22_00025"
     CDS             complement(3318..3626)
                     /locus_tag="PKN22_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS41114.1"
                     /translation="MGFLKKLFGLGPSADFKTLVANGAQIIDVRTPQEFKGGHIKGSV
                     NIPLQNLSTQLSKIKKDKPVITCCASGMRSASAKNILKSNGFQEVHNGGGWMSLNNKI
                     "
     gene            complement(3628..3885)
                     /locus_tag="PKN22_00030"
     CDS             complement(3628..3885)
                     /locus_tag="PKN22_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS41115.1"
                     /translation="MNYKTIIDVRTREEFMGGNVAGSINIPLNEVVDRIEEIRAMEQP
                     ILLCCLSGGRSGQATNYLKSQNIDCENGGGWMDVNYRINNL"
     gene            complement(3882..4079)
                     /locus_tag="PKN22_00035"
     CDS             complement(3882..4079)
                     /locus_tag="PKN22_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNS41116.1"
                     /translation="MKKLFLKYRLALIGLLIGGIGGYAYYYFVGCASGTCAITSKPLN
                     STLYGMLLGVLLFTSFKKEER"
     gene            complement(4085..4285)
                     /locus_tag="PKN22_00040"
     CDS             complement(4085..4285)
                     /locus_tag="PKN22_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNS41117.1"
                     /translation="MKKNMGLLDKVIRISLAILIAILYFSSIITGTTALLLGILAVIF
                     VLTSFVSFCPLYLPFGISTRKD"
     gene            complement(4346..4975)
                     /locus_tag="PKN22_00045"
     CDS             complement(4346..4975)
                     /locus_tag="PKN22_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS41118.1"
                     /translation="MEELKKFETLFEPELMREIAKSASVVQINEGATILDVGQAVKVI
                     PLLLEGILKISRFDESGKELLLYYVHANESCAMTFTCCMQQYPSEIKAVAETNVKLIA
                     IPIGVMDEWLSKYPTWKSFVMKTIRNRFNELLNTIDQLAFQKLDERLVTYLKEKARAT
                     NSMLINLSHEEIANELASSREVISRLLKKLENEKKLLLYRGQIKLLSNL"
     gene            complement(4990..5589)
                     /locus_tag="PKN22_00050"
     CDS             complement(4990..5589)
                     /locus_tag="PKN22_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HNS41119.1"
                     /translation="MACFEFNGFKPVVKPSSFIHPNAVVTGNVIIGENVYIGPGAAIR
                     GDWGQIIIEDGCNVQENCTIHMFPGTTVTLKKGAHIGHGAIVHGGTIGENSMVGMNAV
                     IMDDCVIGKECIIGALAFVPANTTLPDRSLAVGNPAKVIKQVSDEMIAWKTKGTALYQ
                     ALPGECYATLKPCEPLTEVEPNRPTQEKMYQTWEKIKNA"
     gene            complement(5593..6672)
                     /locus_tag="PKN22_00055"
     CDS             complement(5593..6672)
                     /locus_tag="PKN22_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNS41120.1"
                     /translation="MTLAETAELNRRKKIIAIWLLVGVFMIVFQIITGGVTRLTGSGL
                     SMTEWEPIMGAIPPLNDADWQKAFRGYQEIAQYKYVNNHFTIEDFKFIFFWEWFHRLW
                     ARSIGVVFIVPFIYFLVKKYITKDLITPLIIIFGLGVLQATIGWYMVQSGLNGSSLVR
                     VSHVRLAIHLLTALILLAYILWVALRLLIPNDQIVRHSGTRNFHLFTLILLFIQLAYG
                     AFMSGLLAALIAPSWPTMNGYWIPPGMSKLSWINSELNVQFVHRMVAYLLFFVIIFGF
                     IKTWKLAKKTNSITLKKAAIWPLIFVISQVTLGIFTVISIPHLKRGSFGVYETLAQTH
                     QIVGMLLFISIVVVLYIVRGNKSTK"
     gene            complement(6718..9189)
                     /locus_tag="PKN22_00060"
     CDS             complement(6718..9189)
                     /locus_tag="PKN22_00060"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNS41121.1"
                     /translation="MCVPTQLLGQTTPVDTIYINDDSMGGISKYSARDSVYADLKKKQ
                     LHLYGEATVESEDMNLKAGYILIDMNTEEVTATYVIDEEGKMIEKPLFNMGGDQVEAN
                     TLKYNLNTKKAYIEAVKIKQDEMYLYMGKAKRQANEDIHFVDGRFTTCDLDEPHYHFQ
                     LSRAVLVPNKRIVTGPMNLHIAGVPTPLGLPFSYIPQKDQSDRTHGLLFPELVPVSQY
                     GFGLNNLGYYIPINDYVQTSIYGTIYSRGSWGLRNATEYAKRYKYAGNLDVSFEQFRS
                     GFPTNRNLNNVKFAWTHRQDPKSNPKWNFNTNINFISSNNTKNNLDPLNKDYFKNTLL
                     SDINVNRNFPGKPYRLSAKISLNQNSTTKNIALTSPIINFSTNQFFPLKKLLKGEIFQ
                     RLGIIYNIEGQNRSTFKDSLLRTGDFKGIGNQFMYGMRQRITIQTTGGVFKNVLKLTP
                     SVNYGTVLNFQQIRMSHNATTNTAKADTINKVGLAHELSLNMAATTVVYSYYRIVGKN
                     KPIMRHVLTPNVSFSFRPLLTKNRVDSVGYNMAPITYSPFSNSVYSYAATNDQALLNF
                     GINNSFEIKRKSDKDTVTGFQKIRIIDAFSVNGSYDFMRDSMNLSDLNIGLRISPFKW
                     LNIVGASSVSFYGWDATTNASVKQFALQHNKKLGRSMTNSIATTFTITSKESREKIEE
                     NREAAAQNWQTDYDYFYLHPERIIDYRIPWKVNISHQFNLNRNTNKTVYNSAEFNQIH
                     TLNIDADVSITKRWKLGAVLNVDLKNKKVTNGYFTLNRDMHCWALSFRWTPIGYNKSF
                     LLTIRNTSSIFKDAKLDFRKPPVFL"
     gene            9408..10475
                     /locus_tag="PKN22_00065"
     CDS             9408..10475
                     /locus_tag="PKN22_00065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNS41122.1"
                     /translation="MHKAAIKTVVIDAGHGGKDPGCHGAHSNEKTVCLNMALTLGDLI
                     KKNYPEIKVIYTRDKDVFVELDERARIANRNNADLFICIHANSAGPSAYGAETYVLGL
                     HRTESQQKIAERENSTIYLEDDKGEKYKNFDMSPDAIIARQLQLSVFLDQSINFASKL
                     QHEFKSLGRYDRGVKQAGFLVLYKTTMPSVLIESGFLTNVQEENFLGARANQTKMAEA
                     MFKAFKKYKNELEGIHVESDSHPEKPVVNPGKPVVKEENEKIVVAADEKDKVVFRIQI
                     QTSQKRLPLSSKEFKGLTVFEYQQDGLYKYTTGVFINDFKSANNEKNLLRKDGFEHAF
                     VIAFLNGERIDIEKAIKLAEK"
     gene            10485..11522
                     /locus_tag="PKN22_00070"
     CDS             10485..11522
                     /locus_tag="PKN22_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNS41123.1"
                     /translation="MKVSKELKTGVAAVVAIGLLIAGINFLKGNSFFGGDDSYYVYLA
                     DSGGVAPATSIYVNGVIVGKVLDVKLTGKKEENKKVLMRISIQDKDFKIPVGSDIHAG
                     SIDLLSKGLIIEPSSNVASYHKPGDYIQGSLASDLISTVKDYADPLVTKIQDVATSVD
                     KFIVSFQSFWDTTAASELKTTFDDVQVALKKFGNIATDLEGLVASEKVKLSKIFSNVE
                     SITSNLQKSNDKIEGILGNVKTFSDDLVTSDFKGVIAEAKSALTKFNSVLESANNGEG
                     TLGKLLKDEQLYNELNKTNHRLQHLVEDIEAHPERYIHFSVFGAKTKGVPLTPSEERK
                     LKALLDSTNTK"
     gene            11524..12861
                     /locus_tag="PKN22_00075"
     CDS             11524..12861
                     /locus_tag="PKN22_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS41124.1"
                     /translation="MISSIVFGLLLIGGSSFFVWNMLKIRQNILLGREIDRTNNSKDR
                     WKTMFLIAFGQKKMFDRPIAAILHLFIYSAFIITQIELIEIVVDGLTGSHRIFREALG
                     GFYTFLVSFIEILSVLAFVATISFLSRRNLLKLPRFTMREMVGWAKIDGNLILIFEFM
                     LICFIFMMNGADEVLYRQGLSHATNLNDGSLGFSVSQFIGPALFGGMSEGALHVIERI
                     GWWGHIGMVFVFLNYLPYSKHLHILLAFPNTYYSNLEPKGKFTNLDAVTKEVQLMMDP
                     NADPFAAPADGEMAEPQRFGAKDVTDLSWKQLLDSYTCTECGRCSSVCPANLTGKLLS
                     PRKIMMETRDRLAVVGDNIRKHGKDYDDGKSLLYNHITEEELWACTSCNACVQVCPVN
                     IDPLSIIVDMRRYLVMEESKAPSELANMFTNIENNGAPWQFSPTDRLNWKEEQ"
     gene            12883..13671
                     /locus_tag="PKN22_00080"
     CDS             12883..13671
                     /locus_tag="PKN22_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNS41125.1"
                     /translation="MSDLKVPTMAEFAARGEMPDVLFWVGCAGSFDDRAKKVTKAIVK
                     ILTKCNINFAVLGVEEGCTGDPAKRAGNEFLFQMQAMSNIQVLNGYGIKKIVTGCPHC
                     FNTIKNEYPGLGGSYEVVHHSQLINELLQSGELKIEGGDFKGRKITYHDPCYLGRGNN
                     VYEAPRDLIRKLDAELVEMKNCKKKAVCCGAGGAQMFKESEPGKKEINVMRTEEALGT
                     KATIVATGCPFCNTMMTDGVKHFEKEGDVKVMDVAELIANAADL"
     gene            13655..14101
                     /locus_tag="PKN22_00085"
     CDS             13655..14101
                     /locus_tag="PKN22_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41126.1"
                     /translation="MQQIYNLIETFPDTAKVWVYHNQGAIPTAIQPAVQARLDEFSQN
                     WAAHGDKLYGGAVIIEDFFILIFVNEEKTIASGCSIDGSIQFLKKLETEFNLSLFDRM
                     HVVIEENGQKKNVHFADVNQHKGAIFFDPTVSTLGAYKSQWKKVIE"
     gene            complement(14129..14596)
                     /locus_tag="PKN22_00090"
     CDS             complement(14129..14596)
                     /locus_tag="PKN22_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41127.1"
                     /translation="MNNSKKDGSMKWCLFSVSFVLILASCSTFVKVAHTTVQEKDFGY
                     IKTSNATLNFSSLSYADIKYALNSEEYKKLRKGKSPFKHILVYGVATEQPYYEFWVLV
                     GSPQKAINKNNYWVKEKEIDEQLFTLVASKTTPQEDFEFLAAEIKKAITENAQ"
     gene            complement(14589..15713)
                     /locus_tag="PKN22_00095"
     CDS             complement(14589..15713)
                     /locus_tag="PKN22_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNS41128.1"
                     /translation="MKIAIIGGGIGGLTTALALKKVGIPFQLYEQTPAIKAVGAGIIL
                     ANNAMQVYKHLGVHKAIEQAGNKITCMQITNAHLEPLSDLDLQPFEAHYQLQNQAILR
                     SDLHAILVQHVGEENIVLNKRLQNIQKNDEGYQLLFEDGSCATTPFVIGADGIHSKVR
                     QTLFPPSTLRDSHQWCWRGVVNYQLDHPQTAIEAWGQGARCGIVAVNNTQVYWYFTLN
                     DNLGVLQDSPKKHLHRFDKTMEDIILATPQENIIASKLMDLKPINTWNTESVCLIGDA
                     AHATTPNLGQGACQAVEDAYVLGELLKKYDVAKAFQLYPVIRRKKAHFVVNTSWKFGK
                     LAQLNNPFGIFLRNCAVKYLIPKNANKKQLGYLFELDKIE"
     gene            15864..17723
                     /locus_tag="PKN22_00100"
     CDS             15864..17723
                     /locus_tag="PKN22_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS41129.1"
                     /translation="MTFKELGLKSEVLKSLQELGFETPTPIQAEAIPHLLKENSDFVG
                     LAQTGTGKTAAFGLPLVSKVEEGIKIPQGLIICPTRELCLQIAKDLQNYAKHLDINIV
                     AVYGGTDIRRQMKDIKDGVAIVVATPGRLVDLTERKALRLDEVKWVVLDEADEMLNMG
                     FKEDIDTILEKTPFTKNVWLFSATMPKEVAEIAKNYMTNPLEVSIGHKNQTNENIEHI
                     YFMVKERDRYAATKRLIDFNPNIYGLIFCRTRNETAMVAEKLEKDGYNAAPLHGDLSQ
                     AQRDSVMKRFRERSIQVLVATDVAARGIDVSDITHVINYNLPDDIENYTHRSGRTARA
                     GKKGESLVLVNTREMGKIRQIERVIRTTFTAGHVPNAKEICEVQLLQLADKVISAEVK
                     EDDIQEFLPIIMREFDGLTKEEVIKKFVSAEFNRFFDYYQRSGDLNASKEDDRGGRER
                     ERRDRGDSENKTRFFVNLGRRDGLNPGGLLRVICDSTGLRSDNIGRIDIMASFSFFEA
                     DNELVDQILTKANGSDYEGHTVAVEVTRKRQNDGGSRRSSGGRFEGRRSGEGRGDRGG
                     NRFGSRSGEGRSDRSRGDRGSRSNDRGGDRGARSSGFRGRSEGRSGAGDRKRR"
     gene            17738..19534
                     /gene="typA"
                     /locus_tag="PKN22_00105"
     CDS             17738..19534
                     /gene="typA"
                     /locus_tag="PKN22_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNS41130.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDKMIEAGNAVDERERPQGTLIMDN
                     NDLERERGITIVSKNVSVSYKGTKINIIDTPGHADFGGEVERVLNMADGVCLLVDAFE
                     GPMPQTRFVLGKALSMGIKPLLVINKVDKENCTPDEVHEKVFDLMFELDANEEQLEFP
                     TIYGSAKNGWMSEDWKKQTDSITPLLDKILDYFKPKTIEAGTTQMLITSLDYSTFVGR
                     IAIGRLNRGELKPNMPVSVAKRDGSIQKHRIKEVFVFEGLNRVKVESVTAGDICAITG
                     IEGFEIGDCICDIDNPEPLATITIDEPTMSMMFSINDSPFFGKEGKFVTSRHIEERLQ
                     KELEKNLAMRLVKTDKADKWMVFGRGVMHLSVLIETMRREGYELQVGQPQVIIKEIDG
                     VKCEPVEELTIDIPEANSGTAVEAVSKRKGEMKNMEAKGERMIIEFEIPSRGIIGLRN
                     YLLTQTAGEAVVTHRFKEYQPYKGEIPGRQNGSLISMEQGAAIPFSLSNLQERGTFFV
                     NPNESIYEGQVIGEHTRANDLVVNVTKTKKLTNMRASGSDEKVKIAPARVFSLEECLE
                     YIQGDEYVEVTPQNLRMRKILLKETDRKRAGK"
     gene            19662..21068
                     /locus_tag="PKN22_00110"
     CDS             19662..21068
                     /locus_tag="PKN22_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41131.1"
                     /translation="MFDNDDEDFFDEHLREDISNFENYLKGGSMGFMESDRWEFLIDH
                     YLMNSQYKKANLAADYALSQFPYIATYTIRKAQAMSALGQLKEALNLISSVEKNVEDS
                     AELLLTKASIFSQLRDHKQAIKYFKEAIQATQEEEDRDEIFLDLAMEYQALRDFKGAI
                     AVLQEAIRVNPENEGAIYEIAYCYDQLGDFENAIKCYLDFIDENPYSFTAWYNLGNAY
                     TKLDDFDKAIEAYDYSIVINDEFGPVYFNLGNAYMENDRFSDAIEAFQKCIELEGNDP
                     MAFCYIGEAYEQLGNLELAESYYKKSLDIAPLLPDAWLGLGIVEDLKGDTAAGITLIE
                     KASELDPNNAGIYHVLAGAYEKIEDYEKTFDLYDTSLNLDSTDEECLKGFIEFLNKTE
                     TRQAVLEFLETFTTDYGLNDSLDLLMVNQLWLVGRHEEAVALFALCVEKDREKAKELF
                     DINNQLSSVSEFLHLIEK"
     gene            21075..21914
                     /locus_tag="PKN22_00115"
     CDS             21075..21914
                     /locus_tag="PKN22_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNS41132.1"
                     /translation="MNFELPYIPERSVKPRQKGLTMMMDKGLSLQEATNFIQANGHLT
                     DVVKFGFGTAYVTNDLEKKLALYREAGIRPYFGGTLFEVFYARGKHEDYLRLLDKYKM
                     DLVEVSDGSIMINHDEKCEIIHKLAKDRTVLSEVGSKDAGILIAPNRWIKMMKTELEA
                     GSWKVIAEGREAGNVGVFRPNGTAHTMLINKIIAKVKPEDILWEAPQKNQQVWFIKLF
                     GADVNLGNIAPFDVIPLECLRLGLRGDTFFEFLPKDFATRLKQVNDEDEVAEYERENN
                     AEE"
     gene            21934..23220
                     /locus_tag="PKN22_00120"
     CDS             21934..23220
                     /locus_tag="PKN22_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNS41133.1"
                     /translation="MKNLLLLPFVFIFFAFATKAQTYFPPLIGNTWETTDPADLGWCE
                     DSIQKMYNWLENSDSKAFIVLKNGKIVLEKYFGTFTADSFHVWNSAGKTLTAYAVGIA
                     QYENLLNINDKSSDYLGAGWTSLTTSQEDSITIKHQLSMTTGLDDGVADIYCTDASCL
                     VYKAPAGTRWAYHNGPYTLLDSVLKVATGMPLNTWIQQKIGSKIGMQGLYVSVGYNKI
                     FVSKPRSMARFGLLLSQNGYWNGTAVLPDPTYLSEMTNSSQSLNLSYGYLTWLNGKSS
                     FMLPQTQFVFSGSLCPNAPADMYAAEGKNGQIINVVPSQNIVLIRMGASLGNNLVSTT
                     YNDTIWQYMNRLECDLSTKQPEISTWEVAPNPFTNEISISNLSGLETIHITDEWGRAV
                     AFKQFGNRIALSEPKKGIYFLTLSTHQYRKTYKLIH"
     gene            23231..24067
                     /locus_tag="PKN22_00125"
     CDS             23231..24067
                     /locus_tag="PKN22_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HNS41134.1"
                     /translation="MKQLVTISTIVLFIASCASTKISNEQGSITSKQNVREYSVQSVL
                     WQQTAAEYRALCYQAYQTAKLKLDFFLETTKPDNDMGFAIITDIDETVLDNSPYNAWL
                     IENNQDYKFETWKAWTDKASATTVPGAKEFLNYAQSRGVEVFYVSNRTVAEKTTTMAN
                     LKNQGLPYADDAHLLLKSDKSSKLERFKTITASHNILLYIGDNLSDFPSAKPEHSFEV
                     PSTQERNANTDRYNKYFGNYFIVLPNPMYGDWETKGIYQGKHNWTEQQADSLRKRNLR
                     GF"
     regulatory      24153..24268
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            24333..25331
                     /locus_tag="PKN22_00130"
     CDS             24333..25331
                     /locus_tag="PKN22_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNS41135.1"
                     /translation="MHSIENALKERILVLDGAMGTMLQKYGLTEEDFRGKELKDHPQS
                     LKGNNDLLSITQPQIVKEVHRQFLEAGADIIETNTFSATTIAMADYGMENWVYELNFQ
                     SAKIAREVADEFTAQNPQKPRFVAGSIGPTNKTASLSPDVNQPGFRAITFDELKIAYK
                     EQVNALIDGGVDVLLVETIFDTLNAKAALFAIEEVKEERNVSLPIMVSGTITDASGRT
                     LSGQTVEAFLISLSHLPLLTIGFNCALGAQQLHPYLQRLSNMATCGISAYPNAGLPNA
                     FGEYDETPATMCSHVHPYLADGLVNIIGGCCGTTPEHIREIAQEAAKFQPRQLITA"
     gene            25341..28013
                     /gene="metH"
                     /locus_tag="PKN22_00135"
     CDS             25341..28013
                     /gene="metH"
                     /locus_tag="PKN22_00135"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNS41136.1"
                     /translation="MENNYLHLSGLEPLIVTPDSNFINVGERTNVTGSKKFLRLIQEE
                     KYDEALSVARAQVENGAQIIDVNMDEGMLDGKRAMVHFLNLIASEPDISKVPIMIDSS
                     KWEIIEEGLKVIQGKGVVNSISLKEGEQQFIEHAKKIRRYGAAVIVMAFDENGQADSL
                     ERRIEICKRSYDILVNQVQFPAQDIIFDPNIFPVATGLEEHRRNALDFFLATKWIKEN
                     LPHAYVSGGVSNVSFSFRGNPIVREAINSAFLYHAIQHGMQLGIVNPETLTIYDDIPK
                     DLLELVEDVLFDRRDDATERLLDFAEKIKDERSQSKEKVEEWRNGSLQDRITHALVKG
                     IDTYIIDDVEEARLQTANTLDIIEINLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKA
                     VAYLQPFLEAQKTSSANSAGKILLATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVP
                     CDKIIDTAIAENVDMIGVSGLITPSLDEMVFVSKELQRRELSIPLLIGGATTSKAHTS
                     VKIAPEYTESVVHVNDASRAVTIVGKLLGNEKEAFKNEIKEEYDAFRENFLSRKKVKQ
                     YLTFEEANANKLQLNWEQEKIVKPHFLGIQSSQDFDLKTLVDYIDWTPFFKSWELHGK
                     FPDILTDEVVGEQASELYQEAQAMLQKIIDEKLFVAKYIFGIFPANTVNDNDIELYNE
                     QGEVITQFLTLRQQGKKGKGIPNISIADFIAPKATGIQDYIGCFCVSAGFGCDELAQQ
                     FKASNDDFNSILVKALADRLAEAFAEYLHHQIRVDFWGYQPDEKLSNEELIDEAYKGT
                     RPAPGYPACPDHLEKRTIWDLLKVKETIGVKLSEGCAMWPAASVSGYYFANENSKYFG
                     LGKIKDDQLENYAQRRGISLDEARKWLNANYAEE"
     gene            28015..28971
                     /gene="metF"
                     /locus_tag="PKN22_00140"
     CDS             28015..28971
                     /gene="metF"
                     /locus_tag="PKN22_00140"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNS41137.1"
                     /translation="MKVIDKLANRGNNTLFSVEILPPLKGSNLQTIFENIDPLLKFDP
                     SFINVTYQREEFVYKDAGNGLLKKQVVKKRPGTVGICAAIQNKYGIDTVPHILCGGFT
                     KEETENLLIDLDFLGIENVLALRGDAIKSEHYFKPEVEGHAYASELVKQVSELNQGKY
                     LDDELENTSSTKFCIGAACYPEKHMESPSLEQDLYYLKKKVENGADYLVTQMFFDNQS
                     FFEFEKKCRAYGINVPIIPGLKPISVQKHLNLIPHRFHVDLPDDLVKAVMKCKNNDQV
                     KEVGVEWCIQQSKELKEAGVPMLHYYSMGKGDLIERIAAQVW"
     gene            complement(29025..29438)
                     /locus_tag="PKN22_00145"
     CDS             complement(29025..29438)
                     /locus_tag="PKN22_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNS41138.1"
                     /translation="MYPAEVVEPMINQLTSVGFEAITTPEGVDQVINNTNGSLLVVVN
                     SVCGCAAGNMRPGVIESLKNEKTPSKLATVFAGVDNEATAQARKYFLPYPPSSPSIAL
                     FKDGKLVHFLERHHIEGATPEMVAGNLAAAYNQFC"
     gene            complement(29517..30563)
                     /locus_tag="PKN22_00150"
     CDS             complement(29517..30563)
                     /locus_tag="PKN22_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS41139.1"
                     /translation="MKINGHGHILPEPNEVPQFLKDKHLFSIDDDKKFMRQGDWKRPI
                     TDPSFFLQDKLEWMERHQIDHGVMLNLSQIYCNGWSRQDTSDTIRWQNDFNAKVQHEH
                     PDKFTCGFVVQPLHLEDALKEMERSVEKLKLPLLCLPTHFLNADGEWLSVMDESVYPI
                     FELANHYKLAVEIHPYDGEKMVNLKDEYWRFHLVWMMAQTADALHLFSLKDFVSRFPD
                     IRTCFAHGCMLGQANYGRRLQGFDGRPDLFEGTRDPRNTLGHPNVYFDTLVHDAYTLQ
                     LLKIRVGSNQIVAGLDDPYPLGEMEGVARSYPGRVIDYAVKAGIITQQESNEIWSTNV
                     ARWIGKPELLNLKK"
     gene            complement(30587..>30770)
                     /locus_tag="PKN22_00155"
     CDS             complement(30587..>30770)
                     /locus_tag="PKN22_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41140.1"
                     /translation="PSKMEKYMQNNNYMVIYSRHIDELTDEGYHDISSEPITKSIEVV
                     QKIGKGISEIFKKDNQ"
BASE COUNT        10020 a         5251 c         6122 g         9377 t
ORIGIN      
        1 catattctga tacagctgta attgaatacc atgcatgtga tattgaagct ccaaacgtta
       61 tttctttgtc ttctcaatca ggaaacaaca aatggtttgt gaatgctgat ggtattgcag
      121 agtttaactg tgtaattgtt aaccgttggg gtaacgtaat ttatgaatac agcgacttga
      181 ctggttcatg ggatggtaga gatcgttcag gtcatttagt gccagaaggt gtttacttct
      241 acacaattaa agctaaaatt tatggtggag ataaacttga aaaacacgga tttattcaag
      301 tagttcacta ataagattca aataaactaa aagaagaggt tgtcagtagt gataacctct
      361 tttgttttat gtaaatttgt aaacacttta atttggagga catgaaaaca attggtataa
      421 atggcttcgg tagaatagga aggtatttta caaaaatggc cttgcaacaa aatgattttt
      481 ctgttgcgtt ggtaaatgat ttggcagata ttgaaatact tgcccatttg tttaagtacg
      541 atagcgttca tggaagaaac ccatttcaat ttaaagttgt tgagaatgca tttgtgtttg
      601 aaaacggaaa aaaaattgta ttctctcaag aaaaaaatcc agaaaacatt ccatggaaac
      661 aatacaacgt ggacattgtt gttgaagcaa caggtttgtt taaatcaaag gaattagcaa
      721 gtggacacat aaaagccggt gccaaaaaag taattatttc tgccgttcct tcagatgcag
      781 ctattaaaat ggtaacaatg ggcatcaacc aatctatttt ggatggttca gaaacaatta
      841 tttccaacgc atcttgtaca accaataaca tagcaccgct gattgctgtg atgaaaacca
      901 tttgtgaaat tgaaaatgcg tttatcacaa ccgtgcatag ttacacagcc gatcaacgtt
      961 tgcacgatgc tccccacaaa gatttgcgaa gagcacgtgc cgctggtgaa tccattatcc
     1021 caacaacaac tggagccgca aaggctatca ccaagatttt tccagaatac gaaggtaaaa
     1081 tgggaggagg cgcagtgcgt gttcctgtaa aagatggttc tttatccgat atcaccatga
     1141 cgtttaaagg tgatttaacc attgaaaaaa ttaaccaagc aatgaaagca gctgctgatg
     1201 gttatttaaa aggtattttg gaatataccg aagatccaat tgtttctgtg gatgtgcttg
     1261 gaaatccaca tagttgtgta tatgatgctg gtctgacaag tgttgtaggt aacatggtga
     1321 aaattgttgg ttggtacgat aacgaagccg gttactccaa tcgtttggtt gatttgataa
     1381 actatttaaa ataaaattat gaaaagaggt ttgtttccag ggtcttttga tccgtttaca
     1441 aaaggacatg aagtggtggt tagaaaagca ttgaaattgt ttgatgaggt agtgattggt
     1501 gttggaatca atagcactaa aaattatatg tttgataccg aaaaacgtat taagcacatt
     1561 caatcgcttt ttgcagatgc tcccaatgtt agaattgaag cctacaataa attaacggta
     1621 gaattttgta aagacatgga tgcacaattt atcattcgcg ggctcagaga ttcgaaagat
     1681 tatgaatacg agcgctccat tgctcatatg aatcaatcaa tttcggatat agaaacggtt
     1741 atctttttaa ccgaccccga attttctgca attagttcca ccatcattcg tgaaattcat
     1801 agaaatggcg cttccataga gcaatttgta accaattctc atttactcgt ttaattagtg
     1861 gtacaatttc tgctcaggaa ttttaaattt tatcaaatga aacgtctatt tctttccttt
     1921 tttctcctat tgattagctt gtctgttttt ggacaaacca atttggtaac catcaaaggg
     1981 tatgcacctg catttgtagg aaaaaaaatt caagtagaag aaattgaaga ttacatcagt
     2041 caaaaaacag ctaccattgc ttcgtcaact gttgaaagtg attcaacatt ctcaatgtca
     2101 tttaatattg cagaaaccca acgtgtgctt gtgaaaattg agaataaccg ttcttttatg
     2161 tatgttcaac caaaagggca gtattctatt ttatttccac aaaaagaccc tcgtcaacct
     2221 tttcgacctg cgggaaatca agtggaatta actttttttg atttagattc aaccgatatc
     2281 aattacagaa ttttgggttt taaccgttgg atggataatt ttttagcgtt gaattatgcc
     2341 gatgcctata aaaaccctat tcgatttaac cacaaagtgg atagttttaa aacggtagta
     2401 gaaaactatt atgttcaaga tacaggaaaa tacatttttg attacgttcg ttttactatt
     2461 gccaatacca ttgataacat tcagcaagcg ggaaacagaa atagatacga gaagcacgat
     2521 ttttacatca aacaccaaaa agtattgtat cataacgatg cttacatgga ttatttcctc
     2581 aagttttata gtagcatgat gtccatcatt ccaatggaag taaataaccg tgtttattta
     2641 ggattgctaa aaagtagccc ctctttaatt atgaatgcac ttgggttgga atatacgctc
     2701 atcaacagac gtattcgtga aatggtaatg ataaaaatgc tgtcagagtt gtattattct
     2761 cccgattatc ctcaaaccaa catcattacg gttttggata gtgtaaaaaa tcatgcgtta
     2821 ttcagtgcca ataaaataat tgctgccaat ataattaacc gattgactga agtagtaaac
     2881 ggcggaaaag cgcccgattt tgttgtcaaa aatcaaaaag gagatttaaa aggattgagc
     2941 gattataaag gcaagtattt gtacgttcat ttctacaatc ctatggatga aaacaataga
     3001 aaagaaattc cgttgcttca aaatttatac gaaaaataca gcagtgaaat tacatttatc
     3061 actatttgta aagaatcgga tgttgcaaat agtaaggaag acaaaacgtt gaaaaacata
     3121 aaatgggatg ttgcaatagt acccaatgaa aattcaatat tttcttacta taaaattaca
     3181 acattcccac aatatgtatt aattgaccct tattcataca ttgtgcaggc tccagcttta
     3241 ggtccacaac ccaatagcac ctacaaaaca atagaaaaag tgttttttga tattcagcga
     3301 gctataaaac aaaaagatta aatcttgttg tttaaactca tccaacctcc accgttgtgc
     3361 acttcttgaa aaccgtttga tttcaatatg ttttttgcac ttgcgctacg catacccgat
     3421 gcacaacatg taataactgg tttgtctttt ttgatttttg acaattgagt ggataaattt
     3481 tgcaatggaa tattaacaga tcctttgata tgcccgcctt taaattcttg aggcgtacgc
     3541 acgtcaatga tttgtgctcc attggcaact aatgttttga aatcggcact tggacccaat
     3601 ccaaacaatt tttttaggaa tcccatatta caaattgttt attctgtaat taacatccat
     3661 ccatccgcca ccgttttcac agtcgatgtt ttggcttttt aaataattgg tagcttgtcc
     3721 gcttcttcct ccactcaaac agcacaataa aattggttgc tccatcgctc tgatttcttc
     3781 aattctatct accacttcgt ttaatgggat attaatgctt cctgcaacat ttccacccat
     3841 aaattcttca cgtgttctta cgtcgataat tgttttgtaa ttcatctttc ttctttttta
     3901 aaacttgtaa ataataaaac gcctaataac atcccataca aagtgctgtt taatggcttg
     3961 gatgtgatgg cacaggttcc acttgcacaa cctacaaaat aatagtaggc ataaccacca
     4021 attccaccaa ttaacaatcc aatgagggct aatctatatt ttaaaaataa ttttttcaat
     4081 gcttttaatc ttttcttgtt gagataccaa aaggaagata taatgggcag aagctaacaa
     4141 aacttgttag aacaaaaatc accgccaaaa tacctaatag caaagctgtt gttcccgtaa
     4201 tgatagaact aaaatagaga attgcaatta aaatggctaa ggaaatgcga atcactttat
     4261 ccaaaagccc catatttttt ttcaatttca taactttatt tttgacaaaa ttagggcaaa
     4321 tggaagtggt aaaccgtgac aattatcaca aattgctcaa taatttaatt tgaccgcgat
     4381 aaagcaatag ttttttttcg ttctctaact tcttcaatag gcgagagata acttcgcgcg
     4441 atgacgccaa ttcattagca atttcttcat gcgatagatt aatcaacatg gagttggtgg
     4501 cacgtgcttt ttcttttaaa taagtaacca atcgttcatc caatttttga aaggccaatt
     4561 ggtcaatagt gttgagcaat tcattgaaac gattgcgtat ggttttcatt acaaaacttt
     4621 tccaggttgg atatttggac aaccattcat ccatcacccc aataggaatg gcaatgagtt
     4681 taacgttggt ttctgcaact gcttttatct cgctcggata ctgttgcata caacacgtaa
     4741 atgtcatagc acaactttca ttggcatgca cgtaatacaa caaaagctct ttgcctgatt
     4801 cgtcgaaacg agaaattttt aatattcctt caagcaataa tggaattact ttaaccgctt
     4861 gaccaacatc aagaatggtt gcaccttcat ttatttgaac cacacttgcc gattttgcaa
     4921 tctcacgcat taattcgggc tcaaacaagg tttcaaattt ctttagttct tccatattac
     4981 aaaaggcaat taagcatttt taattttttc ccacgtttga tacatttttt cttgtgtcgg
     5041 acggttgggt tccacttctg tcaacggttc acaaggtttt aatgttgcat aacattctcc
     5101 cggcaacgct tgatacaaag ccgttccttt ggttttccat gcaatcattt catcgctcac
     5161 ttgtttaatc acttttgctg gattgccaac tgccaaactt ctgtcgggca acgtagtatt
     5221 agctggaaca aaagccaatg caccaataat gcactctttg ccaataacgc aatcatccat
     5281 aattacggcg ttcattccaa ccatcgaatt ctcaccaata gttccaccat gaacaatggc
     5341 tccatgtcca atgtgcgctc cttttttcaa cgtaacggtg gtgccaggaa acatgtggat
     5401 ggtacaattt tcttgcacat tgcacccgtc ttcaataatg atttgccccc aatctccgcg
     5461 aattgcagct ccgggaccaa tatatacgtt ttcgccaatg atgacgttgc cagttaccac
     5521 cgcgttggga tgaataaaac tcgatggttt tacaactggt ttaaatccgt taaactcaaa
     5581 acaagccatt tcttactttg ttgatttatt tcctctaaca atgtacaata ccaccacaat
     5641 tgaaatgaaa agcaacattc caactatctg atgggtttgt gccaatgttt cgtaaactcc
     5701 aaaactccct ctttttaaat gaggaatgga aattactgta aaaattccta gtgttacctg
     5761 tgaaatcaca aaaatcaaag gccaaatagc tgcttttttc aaagtaatag aatttgtttt
     5821 tttagccaat ttccatgttt ttataaaacc aaagatgatg acaaaaaaca agaggtaagc
     5881 aaccattcgg tgaacaaatt gaacatttaa ttcactatta atccaactca acttagacat
     5941 acctggtgga atccaatatc cattcatcgt aggccaagat ggagcaatta aagcagccaa
     6001 taaccccgac ataaatgcac cgtatgccaa ttgaatgaac aataaaatca atgtgaatag
     6061 gtgaaaattt cgtgttccac tgtgacgcac aatttggtcg tttggaatca ataaacgaag
     6121 tgccacccat aaaatgtaag ctaataaaat taatgccgta agcaagtgaa tggctaaacg
     6181 aacatgacta acacgcacca aactagaacc gttcaatcca ctttgcacca tgtaccaacc
     6241 aattgttgct tgcaaaacac ccaatccaaa aatgatgatt aaaggtgtaa ttaaatcttt
     6301 ggtaatgtat ttctttacta aaaaataaat gaatggaacg atgaaaacaa caccaataga
     6361 acgtgcccat aaacggtgaa accattccca aaaaaagatg aatttaaaat cctcaatggt
     6421 aaaatggttg ttgacgtatt tgtattgcgc aatttcttga taacctctaa atgctttttg
     6481 ccaatctgca tcgtttaatg gtggaatggc tcccataatt ggctcccatt ctgtcattga
     6541 taagcccgaa cctgttaatc ttgttactcc accggttatg atttgaaata caatcataaa
     6601 aactccaacc aacaaccata ttgctatgat tttttttctt cgattgagtt ctgctgtttc
     6661 tgctaatgtc acttgttgtt ttattttaaa tttcaacaaa agtaacaatt ttatgaatta
     6721 taaaaagacg ggtggttttc taaagtctaa cttagcatct ttaaatattg atgaagtgtt
     6781 gcgaatggtt agcaaaaagc ttttgttata accaattggt gtccaacgaa acgaaagtgc
     6841 ccaacagtgc atgtcgcggt tcaaagtgaa ataaccattg gttacctttt tgttttttaa
     6901 atcaacattc aacacggcac ctaatttcca acgtttggta atactcacat cggcatcaat
     6961 gttaagcgta tgaatttgat tgaattcagc agaattgtaa accgttttgt tggtgttgcg
     7021 gttcaagtta aattgatgcg aaatattaac tttccacgga atgcgatagt cgataatgcg
     7081 ttctgggtgc aaatagaaat aatcgtaatc ggtttgccaa ttttgagcgg cagcctctcg
     7141 gttttcttca attttttctc tactttcttt ggaggtgatg gtaaatgttg tggctattga
     7201 attggtcatt gatcgcccta attttttgtt gtgctgcaat gcaaattgtt ttacagaagc
     7261 atttgttgtg gcatcccaac cataaaaaga aaccgaagat gctccaacaa tgttgagcca
     7321 tttaaacgga ctaatgcgca aacctatgtt taggtctgaa agattcatcg aatcgcgcat
     7381 gaaatcataa cttccattca cactaaaagc gtcaattatt ctaatttttt gaaaaccagt
     7441 taccgtgtct ttgtccgatt tacgtttaat ttcaaatgag ttgttgatgc caaaattcaa
     7501 caaagcttgg tcgttagtag ctgcatacga ataaacactg ttagaaaacg gtgaataggt
     7561 gataggcgcc atgttgtagc caacagaatc cactctattt tttgtcaaca aaggtctaaa
     7621 gctaaacgaa acattaggag ttaacacatg acgcatgatg ggtttgtttt ttcccacgat
     7681 gcggtaatag ctgtacacta cagttgtagc agccatgttt aaagaaagtt cgtgtgccaa
     7741 tccaactttg tttattgtat ccgcttttgc cgtattggtt gtagcattgt gcgacattcg
     7801 aatttgttgg aaattcaata ctgttccgta gttgacactt ggcgttaatt tcaaaacgtt
     7861 tttaaataca ccaccagttg tttgaatggt aatacgttgg cgcataccat acataaattg
     7921 attgcctatt cctttaaaat cgcccgtgcg taaaagagaa tctttgaatg tggagcgatt
     7981 ttgcccttca atgttgtaaa taatccctaa acgctgaaaa atctctcctt tcagtagttt
     8041 ttttagagga aaaaactgat tggtagagaa attaataatt ggacttgtta atgcaatatt
     8101 tttggtagtt gagttttgat tcaacgatat tttagccgaa agacgataag gtttgcctgg
     8161 gaaatttcga ttaacattga tatccgaaag caaggtgttt ttaaaataat ctttgtttaa
     8221 tggatcgagg ttgtttttgg tattgttgct cgatataaag ttgatattgg tattaaaatt
     8281 ccatttaggg ttggattttg gatcctgacg atgggtccaa gcaaatttca cattgtttaa
     8341 atttctattg gttggaaatc cacttctaaa ttgttcaaaa gatacgtcta aattccccgc
     8401 atatttatag cgttttgcat attctgttgc attgcgcaag ccccaacttc cgcgtgagta
     8461 aatagttcca tatatcgatg tttgcacata atcgttgata ggaatgtagt accctaaatt
     8521 gttcaaccca aaaccgtatt gagaaacagg taccaactct ggaaacaaca aaccatgcgt
     8581 gcggtcgctt tgatcttttt gcggaatgta gctaaacggt aagcccagtg gggtaggaac
     8641 gcctgcaatg tgcaaattca tcggtccagt aacaatacgt ttattaggaa ccaaaacagc
     8701 tctagaaagt tgaaagtggt aatgtggttc gtctaaatca caagtggtaa agcgaccatc
     8761 aacaaagtga atgtcttcgt ttgcttggcg ttttgctttg cccatataca aatacatttc
     8821 atcttgttta atctttaccg cttcaatgta tgcttttttt gtgttgaggt tgtattttaa
     8881 tgtattagcc tctacctgat cacctcccat gttaaacaaa ggtttttcaa tcatttttcc
     8941 ttcttcgtct ataacatagg ttgccgtaac ttcttcggtg ttcatatcga tgaggatata
     9001 cccagcttta aggttcatat cttccgattc taccgtagct tctccatata aatgcagttg
     9061 ttttttcttt aaatcggcat aaacagaatc gcgcgcactg tatttggaaa ttcctcccat
     9121 gctgtcatcg tttatgtaaa tagtatccac aggagttgtt tgccccaata actgagttgg
     9181 cacacacatt attaacaatg tgtagaagat aaaaatcaat atggaacgtt ttttggacaa
     9241 tctttgaaaa taactttgta atctacattt aacgttttat ggatgcaaaa ctaaaaaata
     9301 acccatttct gaacgcaaaa atatgcggaa tatgcaaaat ctataccttt tttttaggtt
     9361 taggtgtgat cttgctaact aatttctctt attcgcaaaa agatgagttg cataaagcag
     9421 ctataaaaac ggttgtaatt gatgcggggc acggtgggaa agaccctggt tgtcatgggg
     9481 cgcattccaa cgaaaaaacg gtttgtttaa acatggcgtt aaccttaggc gatttaatta
     9541 aaaagaatta tcccgaaata aaagttattt acaccagaga caaagatgtt tttgtggaat
     9601 tggatgaacg tgcacgcatt gccaatagaa ataatgccga tttatttatt tgtattcacg
     9661 caaattcagc tggaccttct gcttatggcg ctgaaactta tgttttgggg ttgcatcgaa
     9721 ctgaatcgca acaaaaaatt gccgagcgtg agaactccac tatttattta gaagacgata
     9781 aaggagaaaa atataaaaac tttgatatgt cacccgatgc cattattgcg cgtcaattgc
     9841 aattgagtgt gttcttggat caatccatca attttgcgag taaattgcaa cacgagttta
     9901 agtcgttggg acgttacgat agaggggtga aacaagctgg atttttggtg ttatacaaaa
     9961 caacgatgcc aagtgttttg attgaatcgg gctttttgac caatgtgcaa gaagaaaatt
    10021 tcttgggtgc acgtgccaat caaacaaaaa tggcagaggc gatgtttaaa gcgtttaaga
    10081 aatataaaaa cgaactagaa ggcatacatg tagaatccga ttcgcatcca gaaaaacctg
    10141 ttgttaatcc aggaaaacct gtagtaaaag aagaaaatga aaaaatagtg gttgctgcag
    10201 atgaaaaaga taaagttgtt tttagaattc aaatacaaac ttctcaaaaa agattacctt
    10261 tgtcgagcaa agaatttaaa ggattaacgg tttttgaata ccaacaagat ggtttataca
    10321 aatacacaac aggtgttttt ataaatgact ttaaatcagc aaataatgag aaaaatttat
    10381 tgcgaaagga tggctttgag catgcttttg tgatagcttt tttaaatgga gagcgcatag
    10441 atatagaaaa agctattaaa ttagcagaaa aataattgag agttttgaaa gtttctaaag
    10501 agttaaaaac aggagttgct gcagtcgtag cgataggctt attaattgcc ggaattaatt
    10561 ttttaaaggg aaatagtttt tttggcggtg atgattcgta ttatgtgtat ctagccgatt
    10621 cgggtggagt agcaccagca acttcaatct acgttaatgg agttattgta gggaaagtgt
    10681 tggatgttaa attgacgggc aaaaaagaag aaaataaaaa agttttgatg cgtattagca
    10741 ttcaagacaa agattttaaa attccagttg gttcggatat tcacgctgga agcattgatt
    10801 tattgagtaa aggtttgatt attgaaccta gctcaaacgt tgcttcttac cataaacctg
    10861 gtgattatat tcaaggaagc ttggctagtg acttgatttc aactgtaaaa gattatgcag
    10921 acccattggt tacaaaaatt caagatgttg ccacatcggt tgataaattc attgtgtcgt
    10981 ttcaatcttt ttgggataca acagcagctt cagaactaaa aactacgttt gatgatgtgc
    11041 aagtagcatt gaaaaagttt ggaaacattg ccaccgactt ggaaggtttg gtagcttcag
    11101 aaaaagtaaa attgtctaag attttctcaa atgtagaatc catcacaagt aatttacaga
    11161 aaagcaacga taaaattgaa ggtattttag ggaatgttaa aactttctct gatgatttgg
    11221 ttacttctga ttttaaaggt gtaattgccg aagcaaaatc tgcattgact aaatttaaca
    11281 gtgtgttgga gtctgcaaat aatggcgagg gaacactagg aaaattattg aaagatgagc
    11341 agttgtacaa cgaattgaac aaaaccaatc atcgtttgca gcatttagtt gaagacattg
    11401 aagcgcatcc agaaagatac attcatttct ctgtttttgg agcaaaaacg aaaggcgttc
    11461 cgttgactcc aagcgaggaa cgtaaattga aagcattgtt agactctaca aataccaaat
    11521 aatatgatta gtagcattgt attcggatta ttgttaattg gcggttctag tttttttgta
    11581 tggaacatgc tgaaaattcg ccaaaacatc ttgttgggta gagaaattga ccgaacaaac
    11641 aattcaaaag atcgttggaa aacaatgttt ttaattgcct ttggtcaaaa gaaaatgttt
    11701 gatcgtccaa ttgcagctat tttgcacctt tttatttatt cggcattcat cattacacaa
    11761 attgagttaa ttgaaattgt agtggatggt ttgacaggaa gtcaccgtat ttttagagaa
    11821 gcattgggtg gattttacac gtttttggtg agtttcattg aaattttatc ggtgcttgct
    11881 tttgttgcca caatttcgtt tttatctcgt cgtaatttat tgaaattgcc tcgatttaca
    11941 atgcgcgaaa tggtaggttg ggcaaaaatt gacggtaacc ttatcttgat ttttgaattc
    12001 atgttgatat gtttcatctt tatgatgaat ggtgccgatg aggtgttgta ccgtcaaggg
    12061 ttatctcatg ccacaaatct taatgatggt tctttagggt tttctgttag tcaattcatc
    12121 ggacctgctt tgtttggtgg aatgtcagaa ggggcacttc atgtaattga aagaataggg
    12181 tggtggggac acatcggaat ggtgtttgtt ttcttaaatt acttgccata ttctaaacac
    12241 ttgcacattt tgttggcttt cccaaatacc tattattcta atttagaacc caaaggtaaa
    12301 tttaccaact tggatgcggt aacaaaagaa gtgcagttga tgatggaccc aaatgcagat
    12361 ccttttgcag ctcctgcaga tggtgaaatg gcagaaccac aacgttttgg agcaaaagac
    12421 gtgacagatt tatcgtggaa acagttgttg gattcatata cctgtacaga atgtgggcga
    12481 tgctcatctg tttgtccggc taatttaacg ggtaaattgt tgtcgccacg taaaattatg
    12541 atggaaaccc gcgacagatt ggctgttgtt ggtgataaca ttagaaaaca cggtaaagat
    12601 tacgatgatg gcaaaagctt gttgtataac catataacag aagaagaatt atgggcatgt
    12661 acgtcgtgca acgcatgtgt gcaagtttgt ccggtaaaca ttgatccatt atctattatt
    12721 gttgatatgc gtcgttactt agtgatggaa gaatccaaag ctccaagtga attggccaac
    12781 atgtttacaa atattgagaa taatggtgct ccttggcaat tctcgccaac agaccgtttg
    12841 aattggaaag aagaacaata agaattttat aaaaaaatat ttatgagtga tttgaaagta
    12901 cctacaatgg ctgaatttgc agctagaggt gaaatgcccg atgttttatt ttgggtaggt
    12961 tgcgccggaa gctttgatga tagagctaaa aaggtaacaa aagcaatcgt gaaaattttg
    13021 acaaaatgca acattaattt tgctgtttta ggagtagaag aaggttgtac gggcgatcca
    13081 gctaagcgtg caggaaatga attcttgttt caaatgcaag ccatgtctaa cattcaagta
    13141 ttgaacggtt atggaattaa aaaaatcgtt accggttgtc cacactgttt caataccatc
    13201 aaaaacgagt atccaggatt gggtgggagc tatgaagtag tgcaccattc tcaattaatc
    13261 aacgaattgt tgcaatcagg tgaattgaaa attgaaggag gtgatttcaa aggaagaaaa
    13321 attacttacc acgatccatg ttatttgggt agaggcaata atgtttatga agcacctcgc
    13381 gatttaattc gtaaattgga tgcggagcta gtagaaatga aaaactgcaa gaaaaaagca
    13441 gtttgttgtg gagctggtgg ggcacaaatg tttaaagagt ctgaacctgg taaaaaagaa
    13501 atcaatgtga tgcgaacaga agaagcattg ggaacaaaag caacaattgt tgcaacaggt
    13561 tgtccattct gtaatacaat gatgacagat ggcgtaaaac actttgaaaa agaaggtgat
    13621 gttaaagtaa tggatgtagc agaattaata gcaaatgcag cagatttata atttaataga
    13681 aacgttccca gacacagcaa aagtttgggt atatcataac caaggagcaa ttccaacagc
    13741 aattcaacca gcagtgcaag cacgtttgga tgaattttcg caaaattggg ctgctcacgg
    13801 tgataaattg tatggaggag cggttattat agaggatttc ttcatactta tatttgtgaa
    13861 cgaagaaaaa acaattgctt cgggttgctc aattgatggt tccattcaat ttttgaaaaa
    13921 actggaaact gaattcaact tgagtttgtt tgaccgtatg cacgttgtaa ttgaagaaaa
    13981 tggacaaaag aaaaacgtac attttgcaga tgttaatcaa cacaaaggcg ccatcttttt
    14041 tgatccaacc gtaagcacat tgggtgcata taagtcgcaa tggaaaaaag tgattgaata
    14101 aaattaaata aattcttatt taaggcaatt attgagcatt ttcagtaatt gcctttttta
    14161 tttctgctgc caaaaactca aaatcttctt ggggcgttgt ttttgatgca acaagggtaa
    14221 atagttgctc atctatttct ttttccttta cccaatagtt gtttttatta attgcttttt
    14281 gaggcgaacc aacgagtacc caaaattcat aatacggttg ttcggttgct acgccataaa
    14341 ccaaaatgtg cttaaacggc gatttacctt ttcttaattt tttgtattcc tcactgttga
    14401 gcgcatattt aatgtcagca tacgataaac ttgaaaaatt taatgttgcg ttacttgttt
    14461 tgatataacc aaaatccttc tcttgtacgg ttgtgtgggc aactttcaca aaggttgaac
    14521 aactagcaag tatcaaaaca aacgatactg aaaataaaca ccacttcata cttccatctt
    14581 ttttagaatt attcaatttt atcaagttca aacaagtaac ctaattgttt tttgttagca
    14641 ttcttaggaa tcaggtactt aacagcacaa ttgcgtaaaa atattccaaa agggttgttt
    14701 aattgagcta attttccaaa tttccaactg gtgttaacca caaaatgtgc ttttttgcga
    14761 cgaattacag gatatagctg aaaagcttta gcaacatcgt attttttcaa taattcaccc
    14821 aacacataag catcttcgac ggcttgacaa gcaccttgac ccaaattagg ggttgttgca
    14881 tgagctgcat ctccaattaa acaaacactt tcggtgttcc atgtatttat tggttttaaa
    14941 tccattaatt tagaggcaat aatgttttct tgcggtgttg ccaaaatgat gtcttccatt
    15001 gttttgtcaa atcgatgcag gtgtttcttt ggtgagtctt gaagaactcc tagattatca
    15061 ttaagtgtaa aataccaata aacttgggtg ttatttactg caactatgcc acaacgagcg
    15121 ccttgccccc acgcctcaat ggcagtttgt gggtgatcca attggtaatt tacaactccc
    15181 cgccaacacc attgatgcga atcacgcagt gtggaaggtg ggaataaagt ttgacggact
    15241 ttggaatgaa tgccgtcagc tccaataaca aagggtgtag ttgcacaact tccatcttca
    15301 aacaaaagtt gatagccttc atcatttttt tgaatatttt gcaagcgttt gtttaataca
    15361 atgttttcct cgccaacatg ttgcaccaaa atagcatgca aatcagatcg taaaatggct
    15421 tgattttgaa gttgatagtg tgcttcaaac ggttgtaaat ccaaatcaga tagtggttcc
    15481 aaatgtgcat tggtgatttg catgcaagta attttgttcc ctgcttgttc aatggcttta
    15541 tgaacgccta aatgcttgta aacttgcata gcattattgg ctaaaatgat acctgctcca
    15601 acggccttga ttgcaggtgt ttgctcgtat aattgaaacg gaatcccaac cttttttaat
    15661 gccaatgcag tggttaatcc gccaattcct cccccaataa ttgcaatttt cataacctga
    15721 tcaatttcta tcaaaaataa aaaaacaaat taattaccaa ttttttatca cctaaaaatt
    15781 acagctttta tatttatttt tcatagttta ttgtgtaaca agaaattctc cgtacctttg
    15841 cgccctattt taaaatattt tagatgacat ttaaagaact cgggctaaag agtgaggtgt
    15901 tgaagtcgtt acaagaattg gggtttgaaa cacctactcc tatacaagcc gaagcgattc
    15961 ctcacttgtt aaaagaaaac agtgatttcg ttggcttagc tcaaacaggg acaggaaaga
    16021 cagcagcgtt tggtcttcca ttagttagta aagttgaaga aggaattaaa atcccacaag
    16081 gattaatcat ttgtcccaca agggaattat gccttcaaat tgcaaaagat ttacaaaatt
    16141 acgcaaagca tttagacatt aatattgttg ccgtttacgg aggaacagat atccgccgtc
    16201 aaatgaaaga tattaaagat ggcgtggcaa ttgttgttgc tacacctggt cgtttggttg
    16261 atttaacaga acgcaaagca cttcgtttgg atgaagtgaa atgggtagtg ttggatgaag
    16321 ccgacgaaat gttgaacatg gggttcaaag aagatatcga tacgatactt gaaaaaacgc
    16381 catttacaaa aaatgtttgg ttattttctg caacaatgcc aaaagaagtg gcagagattg
    16441 ctaaaaatta catgacaaat ccactagaag tgagcattgg tcataaaaac caaaccaatg
    16501 aaaatattga gcacatctat ttcatggtta aagaaagaga tagatatgca gcaacaaaac
    16561 gtttgattga ctttaaccca aatatttatg ggttgatttt ctgtcgcaca cgtaacgaaa
    16621 ctgcaatggt tgccgaaaaa ttagagaaag acggatacaa tgccgctcct ttgcacggtg
    16681 atttatcgca agcacaacgt gactcagtaa tgaaacgttt tcgtgagcgt tccatccaag
    16741 tgttggttgc aactgacgta gcagctcgtg gtatcgacgt gagcgatatt acgcacgtaa
    16801 tcaattataa tttaccagac gatattgaaa actatacgca ccgttctggt cgtacagctc
    16861 gtgcaggtaa gaaaggagaa tctttggtat tggtgaatac ccgtgaaatg ggtaaaattc
    16921 gtcaaattga acgtgttatt cgcactactt ttactgcagg tcacgtacct aatgcaaaag
    16981 aaatttgtga agttcaattg ttgcaattag ccgataaagt tatttctgcc gaagtaaaag
    17041 aagacgatat ccaagaattc ttgccaatta ttatgcgtga atttgatggt ttgacaaaag
    17101 aagaagtcat taaaaaattc gtttctgcag agttcaaccg tttctttgat tattaccaac
    17161 gttctggtga tttgaatgct tcgaaagaag acgatagagg tggacgtgaa cgcgaacgta
    17221 gagatagagg tgattctgaa aataaaacac gtttcttcgt aaacttaggg cgtagagatg
    17281 gtttaaatcc aggtggattg ttgcgtgtga tttgcgattc aacaggattg cgctcagaca
    17341 acatcggtcg aattgacatc atggcatcgt tctcattctt tgaagccgat aatgaattgg
    17401 tagatcagat tttgacaaaa gcaaacggtt ctgattacga aggacacacc gttgctgttg
    17461 aagtgactcg taaacgccaa aatgatggtg gaagcagaag aagcagtgga ggtcgctttg
    17521 aaggtcgtcg ttctggagaa ggaagaggcg atagaggtgg taaccgtttt ggaagccgtt
    17581 ctggagaagg tagaagtgat agaagccgtg gagaccgagg aagcagaagc aacgatagag
    17641 gtggtgatag aggcgcaaga agtagtggct ttagaggcag aagtgaaggt cgctcaggcg
    17701 ctggagacag aaaaagaaga taaaacaaca ataatttatg gaaattagaa acatagccat
    17761 cattgcgcac gttgaccacg gtaaaacaac attggttgat aaaatgattg aagcgggcaa
    17821 tgctgttgat gaaagagaac gtccgcaagg aacattgatt atggataaca acgatttgga
    17881 acgtgaacgt ggtattacca tcgtttctaa aaacgtttcg gtatcgtata aaggaacaaa
    17941 aattaatatc attgacactc cggggcacgc cgattttggt ggtgaggtag aacgtgtatt
    18001 gaacatggcg gatggtgttt gtttgttggt ggatgctttt gaaggtccaa tgccacaaac
    18061 acgcttcgta ttaggaaaag ctttgtcaat gggaattaaa ccgttgttgg taattaacaa
    18121 agtggacaaa gaaaactgta caccagacga agtgcacgaa aaagtgttcg atttgatgtt
    18181 tgaattggat gccaacgaag aacaattgga attccctaca atctacggtt ctgctaaaaa
    18241 cggttggatg tctgaagatt ggaaaaaaca aacagattcg attactccgt tattagacaa
    18301 aattttagat tactttaaac ctaaaacaat tgaagcgggt acaactcaaa tgttgattac
    18361 atcgttggat tattcaacct ttgttggacg tattgccatt ggtcgtttaa accgtggtga
    18421 attgaaacca aacatgccag tttctgttgc aaagcgcgat ggttctatcc aaaaacaccg
    18481 catcaaagaa gtgtttgtgt ttgaaggatt gaacagagta aaagtagaaa gcgtaacagc
    18541 aggagatatt tgtgcaatta caggtattga aggatttgaa attggtgact gcatttgtga
    18601 tatcgataat ccagaaccat tggcaacaat cactattgat gagccaacaa tgagtatgat
    18661 gttttctatc aacgattcac cattctttgg aaaagaagga aaatttgtga cttcgcgcca
    18721 tattgaagaa cgtttgcaaa aagagttgga aaaaaacttg gcaatgcgtt tggtgaaaac
    18781 agataaagcc gacaaatgga tggtgtttgg tcgtggtgtt atgcacttgt ctgttttaat
    18841 tgaaacgatg cgtcgtgaag gatatgaatt gcaagttggt cagccacaag ttatcatcaa
    18901 agaaattgat ggtgtaaaat gtgagccagt tgaagagttg acaattgata ttcctgaagc
    18961 aaattctgga acagctgtag aggcagtttc taagcgcaaa ggtgaaatga aaaacatgga
    19021 agcaaaaggt gagcgaatga ttatcgaatt tgaaattcca tcaagaggta tcattggttt
    19081 aagaaactat ttgttgacac aaacggcagg tgaagcagtg gtaacacacc gttttaaaga
    19141 atatcaaccg tataaaggag aaattcctgg acgtcaaaat ggttcattga tttctatgga
    19201 gcaaggtgct gcaattcctt tctctttgtc taacttacaa gaaagaggaa cgtttttcgt
    19261 gaatccgaac gagtcgattt acgaaggaca agtaattgga gaacatacaa gagcaaatga
    19321 cttggtggtt aacgtaacaa aaacgaaaaa attaaccaac atgcgtgctt ctggttccga
    19381 tgagaaagta aaaattgcac cagcaagagt tttctcttta gaagaatgtt tagaatacat
    19441 ccaaggagat gagtatgtgg aagttacgcc tcagaatttg cgtatgcgta aaatcttgtt
    19501 aaaagaaaca gatagaaaaa gagcaggtaa ataagctttt ttattcaaat ttatagatga
    19561 acagtctctt ttggggctgt tcattttttg ttaataatta atcgaaacga aatagttaag
    19621 tgcatttttt tttgtaactt tatagttaaa tagaaagttc tatgtttgat aacgacgatg
    19681 aggatttttt tgacgagcat ttgagagaag atatttcaaa ttttgaaaac tatctcaaag
    19741 gtggttcaat gggatttatg gaaagcgatc gctgggagtt tttgattgac cattacttga
    19801 tgaatagtca gtacaaaaaa gccaatttag cagcagatta tgccttgtca cagtttccat
    19861 acatcgcaac ctataccatt cgaaaggcgc aagctatgtc ggcattagga caattgaaag
    19921 aagcattgaa tttgatttca agcgttgaaa aaaacgtaga agattctgca gaactattgc
    19981 taaccaaagc ttctattttt agtcaattac gcgatcacaa gcaagcaatt aaatatttta
    20041 aagaggcaat acaagctaca caagaagaag aggataggga tgaaattttc ttggatttag
    20101 caatggaata tcaagccttg cgtgatttca aaggtgctat tgctgttttg caagaagcca
    20161 tccgtgtgaa cccagaaaat gaaggagcaa tttatgaaat agcatattgc tatgatcaat
    20221 tgggtgattt tgaaaatgcc atcaaatgtt atcttgattt tatagacgaa aatccctatt
    20281 cgtttaccgc ttggtataat ttaggaaatg cctatacgaa attagatgat tttgataaag
    20341 caatagaagc ctacgattat agcattgtga ttaacgatga atttggcccc gtttatttca
    20401 atcttggaaa tgcttacatg gaaaatgatc gtttctcgga tgcaatagaa gcttttcaaa
    20461 aatgcataga attggaagga aacgacccga tggccttttg ttatattgga gaggcgtatg
    20521 agcaattggg aaatttagaa ttggctgaaa gttattacaa aaaaagtttg gatattgccc
    20581 cattgttgcc tgatgcttgg ttgggattgg gaattgttga agacttaaaa ggcgatactg
    20641 cagctggaat tactttgatt gaaaaagcat ccgaattaga ccccaataat gcgggtattt
    20701 accacgtgtt ggcgggtgcc tatgaaaaaa ttgaagatta cgaaaaaacg tttgatttat
    20761 acgatacatc actcaacttg gatagcactg atgaagaatg cttgaaagga tttattgaat
    20821 ttttaaacaa aacagaaaca cgtcaagcag tattggagtt tttggagacg tttacaaccg
    20881 attacggcct caacgattcg ttggatctat tgatggtcaa tcaattgtgg ttggttgggc
    20941 gccacgaaga agcagtagca ttgtttgctt tatgtgttga aaaagataga gaaaaagcaa
    21001 aagaactatt tgatataaac aatcaattga gctccgttag tgagttttta catttaatcg
    21061 aaaaataaaa acctatgaat tttgaattac catacattcc agaaagatct gtaaaaccaa
    21121 gacaaaaagg attaacaatg atgatggata aaggattgag cttgcaagaa gctaccaact
    21181 ttattcaagc aaatggtcat ttaacagatg ttgtaaaatt tggatttgga actgcatatg
    21241 ttaccaatga tttagaaaag aaattagcac tttaccgcga agcaggcatt cgtccgtatt
    21301 ttggtggaac tttgtttgaa gtattttatg caagaggcaa acatgaagat tatttgcgtt
    21361 tgttggataa atacaaaatg gatttggtgg aagtttcgga tggttcaatt atgattaacc
    21421 acgatgagaa atgtgaaatc atccataaat tagccaaaga tagaacggtt ttatcagaag
    21481 tgggctccaa agatgccggt attttaattg ctccaaatcg ttggattaaa atgatgaaaa
    21541 cagagttgga agctggttct tggaaagtga ttgcagaagg tagagaggct ggaaacgttg
    21601 gtgtttttcg tccaaatgga accgctcata ccatgttgat taataaaatc attgccaaag
    21661 ttaaacccga agatatttta tgggaagcac cacaaaaaaa ccaacaagtg tggttcatca
    21721 aattgtttgg tgctgacgtg aatttaggaa atattgctcc ttttgacgtt attccgttag
    21781 aatgtttgcg cttaggttta agaggcgata cgttttttga gtttttacca aaagattttg
    21841 ctacgcgctt aaaacaagtt aatgatgaag acgaagtggc tgaatacgag cgtgaaaaca
    21901 atgcagaaga ataaacttta gttttatttt aagatgaaaa atctattgct acttccattt
    21961 gttttcatct tttttgcgtt tgcaacaaaa gcacaaacgt attttccacc gttaataggc
    22021 aatacgtggg aaacaaccga tcctgctgat ttgggctggt gcgaggattc cattcaaaaa
    22081 atgtacaatt ggttagaaaa ttccgattct aaagcattta ttgtactaaa aaatggaaaa
    22141 attgtgcttg aaaaatactt cggtactttt acagctgata gtttccatgt ttggaattcg
    22201 gcaggaaaaa cattaaccgc ttatgccgtt ggtattgctc aatatgaaaa tctgttgaat
    22261 atcaacgata agtcaagcga ttatttaggt gcaggttgga ccagtttaac aacgtcgcaa
    22321 gaagattcaa ttacaatcaa acatcagttg agcatgacta caggacttga cgatggtgtt
    22381 gcagatattt attgcaccga tgcaagttgc ttggtctata aagctcctgc gggcacacgt
    22441 tgggcatatc acaatggtcc ctatacctta ctcgattctg ttttgaaagt tgcaacagga
    22501 atgccattaa atacttggat tcagcaaaaa ataggttcca aaattggtat gcaagggttg
    22561 tatgtaagtg ttggatataa caaaattttt gtttctaaac cacgttccat ggctcgcttt
    22621 ggcttgcttt tatcacaaaa tggttattgg aacggaacag ccgtattgcc cgatccaacg
    22681 tatttaagtg aaatgaccaa ttcgtcgcaa tcgttgaacc tttcgtatgg ttacttaaca
    22741 tggttgaacg gcaaatcatc gtttatgttg cctcaaactc aatttgtttt cagtggttcg
    22801 ttgtgtccca atgcaccagc agatatgtat gcagccgagg gtaaaaatgg gcaaattatc
    22861 aatgtcgttc catcacaaaa tatcgttttg attcgtatgg gagcttcgtt gggaaataat
    22921 ttggtttcta ccacctacaa cgataccatt tggcaataca tgaaccgttt ggagtgtgat
    22981 ttaagtacaa aacaacccga aatttctaca tgggaagttg cacccaaccc attcaccaat
    23041 gaaatttcta tttcaaattt atctggactt gaaacaattc acattacaga tgaatgggga
    23101 agagcagttg cttttaaaca atttggtaac cgcattgcac tttctgaacc taaaaaagga
    23161 atttattttc taactttatc aactcatcaa taccgaaaaa catacaagtt aatccattaa
    23221 tacacataca atgaaacaat tagtaacaat ttcaactatc gttcttttta ttgccagctg
    23281 tgcaagtaca aaaatttcaa atgaacaagg ttcaataacc tccaaacaaa atgttagaga
    23341 atactcggtt caatcggtgc tttggcaaca aacagcagcc gaataccgag cattgtgtta
    23401 tcaagcctat caaacagcaa aactaaagtt ggattttttc ttggaaacaa ccaaaccaga
    23461 caatgacatg ggctttgcaa taattacaga tattgacgaa accgttttgg ataacagtcc
    23521 ctacaacgca tggttaattg aaaataacca agattataaa tttgaaactt ggaaagcatg
    23581 gacagacaaa gcaagcgcta caactgttcc tggagctaaa gaatttttaa attacgccca
    23641 aagcagaggt gttgaggtgt tttatgtgag caacagaaca gttgcagaaa aaacaacaac
    23701 catggctaat ttgaaaaacc aaggtttacc ttatgccgat gatgcacatt tgcttttaaa
    23761 atcggataaa agttcaaagt tagaacgctt taaaacaatt acagcttcgc acaatatttt
    23821 gttgtacata ggcgacaatc tttctgattt tccatcagca aaaccagaac attcttttga
    23881 agttccttca acacaagagc gcaatgctaa taccgaccga tacaacaagt attttggaaa
    23941 ctattttatt gtattgccca atccaatgta tggtgattgg gaaactaaag gaatttatca
    24001 aggaaaacac aactggaccg aacaacaagc agattctctt agaaaaagaa atttgagagg
    24061 gttttaggaa aaaatctatt tttttgttaa tttatttggt gttttgctaa aaagtagtga
    24121 attttgtgcc cacagagttc atttatatag taaagttata aagaaaggcg gagggaatag
    24181 acccaaagaa accttagcaa ccctttgagt aagcttactt gaagaaggtg ctaaattcta
    24241 ccctaaattt tttagggaga gataacagca acatcatttc ttatatcttt ttattgaatt
    24301 gttctctaat tattaatttt aattagtgaa gaatgcacag tattgaaaat gcattaaaag
    24361 aacgtatttt agttctggac ggcgccatgg gaaccatgct ccaaaagtat gggttgaccg
    24421 aagaagattt tcgcggaaag gaattaaaag accacccgca atcgttaaaa ggcaacaacg
    24481 atttattgtc gattacccaa ccacaaattg taaaagaagt gcaccgccaa tttttggaag
    24541 caggtgccga tatcatcgaa accaatacat tttcggcaac aactatcgct atggccgatt
    24601 atggtatgga gaattgggtg tatgaactca actttcaaag tgctaaaatt gcccgtgaag
    24661 tagccgatga atttactgca caaaatccgc aaaaaccacg ctttgtggca ggaagcattg
    24721 gtccaacaaa taaaacagcc agcctttcac ccgatgttaa ccaaccagga tttagagcca
    24781 tcacttttga tgaattgaaa attgcctata aagagcaagt taatgccctc atcgacggtg
    24841 gtgtagatgt gttgttggtt gaaaccatat ttgatacgct caacgcgaaa gcggcacttt
    24901 ttgccattga agaagtaaaa gaagaacgca atgtttcttt gcccatcatg gtttctggaa
    24961 caataacaga tgcttcgggc agaaccttgt cgggacaaac cgtagaggca tttttaattt
    25021 cattgtcgca cttgcctttg cttaccattg gttttaactg tgcacttggt gctcaacaat
    25081 tgcatcccta tttgcaacgc ttatccaata tggcaacatg tggaatttct gcctatccta
    25141 atgctggatt gccgaatgct tttggagaat acgatgaaac tcctgcaaca atgtgctcgc
    25201 atgttcatcc ttatttagcg gatggtttag taaacattat tggtggatgc tgtggaacaa
    25261 ctcccgaaca cattcgagaa attgcccaag aagcagcaaa atttcaacct cgtcaattaa
    25321 taactgcata acgaaagaag atggaaaaca attatttaca cctttcaggg ttagagccat
    25381 taatcgttac gccagatagt aattttatca atgttggtga acgaactaac gtaacaggtt
    25441 ctaaaaaatt cttgcgctta atccaagaag aaaaatacga tgaagcattg agcgttgcgc
    25501 gtgcacaagt agaaaatggt gctcaaatca tcgatgtgaa tatggacgaa ggaatgttgg
    25561 atggaaaacg tgccatggtg catttcctca acctcattgc ttctgaacca gatatttcca
    25621 aagtgcccat tatgattgat agctctaaat gggaaatcat tgaagagggc ttaaaagtta
    25681 ttcaaggcaa aggtgttgtt aattccatca gtttaaaaga aggagaacaa caatttattg
    25741 agcatgctaa aaaaatcaga cgttatggtg ccgctgtgat tgtcatggca tttgatgaaa
    25801 acgggcaggc agattcgttg gagcgacgca ttgaaatatg taaacgttcc tacgatattt
    25861 tggtcaacca agtgcaattt cctgctcaag acatcatttt tgaccccaat attttccctg
    25921 ttgcaaccgg attagaagaa caccgaagaa atgccttgga ttttttcttg gcaaccaaat
    25981 ggatcaaaga aaatttgcct catgcctacg tatcaggcgg tgtgagcaat gtttcgtttt
    26041 cgtttcgtgg caatccaatt gtgcgcgaag ccatcaactc tgcgttttta taccacgcca
    26101 ttcagcacgg aatgcagttg ggaattgtta acccagaaac cttaaccatt tacgacgata
    26161 ttcccaaaga tttgttggaa ttagtggaag atgtgttgtt tgaccgacgt gatgatgcta
    26221 ccgaacgttt attggatttt gcagaaaaaa tcaaagatga acgcagtcaa tccaaagaaa
    26281 aagtggaaga atggcgcaat ggttctttgc aggatagaat tacacacgca ttggtaaaag
    26341 gaattgacac ctacattatt gacgatgtgg aagaagcccg tttgcaaact gccaatacac
    26401 tggatatcat tgaaatcaac ttgatggctg gaatgaatgt ggtgggcgat ttgtttggaa
    26461 gtggaaaaat gtttttaccg caagttgtaa agtcggcacg tgttatgaaa aaagcagtgg
    26521 cttatttgca accgttttta gaagctcaaa aaacaagtag tgccaattct gctgggaaaa
    26581 ttttgttggc aacagtaaaa ggcgatgtgc acgacattgg aaaaaatatt gttggagtag
    26641 tgttggcgtg taataactat gaaatcattg atttgggagt aatggtgcca tgcgataaaa
    26701 tcattgatac ggccattgca gaaaacgtag atatgattgg cgttagtggt ttgattactc
    26761 catcattgga tgaaatggta tttgtatcta aagaattgca acgtcgtgag ctttctattc
    26821 cattgttgat tggcggtgca accacttcaa aagcacatac ttctgtaaaa attgcaccag
    26881 aatacaccga atcagtagtt catgtcaacg atgcctcaag ggcagttaca atagtaggaa
    26941 aattattggg caacgaaaaa gaagcgttta aaaacgaaat caaagaggaa tacgatgctt
    27001 ttcgtgaaaa cttcttgtcg cgcaaaaaag taaaacaata cctcactttt gaagaagcca
    27061 atgccaataa attgcaattg aattgggaac aagaaaaaat tgtaaaacca cattttttag
    27121 gtattcaatc atcgcaagat tttgatttaa aaaccttggt tgattacatt gattggacac
    27181 cttttttcaa aagttgggag ttacacggaa aatttccaga cattctaacc gatgaggtgg
    27241 taggcgagca agcatcggag ttgtatcagg aggcacaagc catgttgcaa aaaatcattg
    27301 atgaaaaatt gtttgtagcc aaatatattt ttggaatttt cccagccaat acagttaatg
    27361 acaacgacat tgaactgtat aacgaacaag gcgaggtaat tactcaattt ttaacccttc
    27421 gtcagcaagg aaaaaaagga aaaggtattc ctaatatttc aattgccgat tttatagcac
    27481 cgaaagcaac tgggattcaa gattacattg gttgtttttg tgtgtctgct ggttttggtt
    27541 gcgatgaatt ggcacaacag ttcaaagcat ccaacgatga ttttaattcc attttggtga
    27601 aagcactagc cgaccgttta gccgaagctt ttgctgaata cttgcaccat caaatacgcg
    27661 ttgatttttg gggctatcaa cctgatgaaa aattgagcaa tgaagaattg attgatgaag
    27721 cctacaaagg cactcgtcct gcaccaggtt atcccgcatg tcccgaccat ttggaaaaac
    27781 gtaccatttg ggatttgtta aaagtaaaag aaacaattgg tgttaaattg tcggaaggtt
    27841 gtgccatgtg gccagctgca tcggtgagtg gttattattt tgccaacgaa aactccaaat
    27901 attttggtct aggtaaaatt aaagacgatc aattggaaaa ttatgcacaa cgaagaggca
    27961 tttcgttgga tgaagcaaga aaatggttaa acgcaaatta cgcagaagaa taaaatgaaa
    28021 gttattgata aattagcaaa tagagggaac aatacgttgt tttccgttga aatattgcca
    28081 ccgttaaaag ggagcaattt acaaacaatt tttgaaaata ttgacccttt gttgaagttt
    28141 gatccttcgt ttatcaacgt aacgtatcag cgcgaagaat ttgtgtacaa agacgcaggc
    28201 aacggtttgt tgaaaaaaca agtggtgaaa aaacgtcccg gaacagtagg tatttgtgct
    28261 gccattcaaa acaagtatgg aatagacacc gtgccgcata ttttatgtgg aggatttacc
    28321 aaagaagaaa ctgaaaattt gttgatagat ttggatttct taggaataga aaacgtactg
    28381 gctttgcgtg gcgatgcaat taaaagtgaa cattatttca agccagaagt agagggacat
    28441 gcctatgcat ccgagttggt gaaacaagta agtgaactca atcaaggaaa atatttagac
    28501 gatgaattgg aaaatactag ttctacaaaa ttttgcattg gagcagcttg ttaccccgaa
    28561 aaacacatgg aatcgccaag tttggaacaa gatttatact acctaaaaaa gaaagttgag
    28621 aacggcgccg attatttggt aacccaaatg ttttttgata accaatcgtt ttttgaattt
    28681 gaaaagaaat gcagagcgta tggcatcaat gttccaatca ttccggggct aaaaccaatt
    28741 tctgttcaaa agcacttgaa tttaattcca caccgttttc atgtggattt acccgatgat
    28801 ttggtaaaag cggtaatgaa gtgtaaaaac aacgatcaag tcaaagaagt gggagtggag
    28861 tggtgcattc agcaatccaa agagttgaaa gaggctggcg ttccaatgtt acattattac
    28921 agcatgggca aaggcgattt aattgaacgc atagccgctc aggtgtggta ggaatataaa
    28981 aaaagccctc tttttgggag ggctttaaat tttttagaat tttattagca aaactgattg
    29041 tatgcagctg ctaagtttcc tgctaccatt tctggagttg caccttcaat gtggtgacgc
    29101 tctaagaaat gtaccaactt accatctttg aacaaagcaa tagatggaga agatggaggg
    29161 taaggaagaa aatattttct tgcttgtgca gttgcttcgt tatcaacccc tgcaaaaact
    29221 gttgctaatt tagatggtgt tttttcattt ttcaacgact caattacacc aggacgcata
    29281 tttcctgctg cacatccaca tacagaattt acaacaacaa gcaaacttcc gtttgtatta
    29341 ttaattactt ggtcaactcc ttctggcgta gtgattgctt caaaccctac ggaagttaac
    29401 tgatttatca ttggttcaac tacctctgct ggatacataa tatatttgat ttaaaattta
    29461 gcacaaagat actaaaaagt cttaagaagt aaaagcaatt ctttgtttca actattttac
    29521 tttttcaagt ttaaaagttc tggttttcct atccaacgcg ccacgttagt gctccaaatt
    29581 tcgttagact cttgttgggt gatgatgcct gcttttaccg cataatcgat tactcgccca
    29641 gggtaagaac gcgccacacc ttccatttct cccaatggat aaggatcatc caaaccggcc
    29701 acaatttgat tggagcccac tctgattttc aaaagttgca acgtataggc atcgtgcacc
    29761 aatgtgtcga aataaacgtt gggatgtccc aaagtattgc gagggtctct tgtgccttca
    29821 aacaaatctg ggcgtccgtc aaatccttgc aatcttctac cataattggc ttgccccaac
    29881 atacagccat gagcaaaaca agtgcgtata tctgggaaac gcgaaacaaa atcttttaac
    29941 gaaaataagt gcaaagcatc ggctgtttgt gccatcatcc aaaccaagtg aaaacgccag
    30001 tattcgtctt taagattcac cattttctca ccgtcgtagg ggtggatttc aacagccaat
    30061 ttgtaatgat tagccaattc aaaaatagga tacaccgatt cgtccatcac cgaaagccat
    30121 tctccatcag catttaaaaa atgtgtcggt aaacaaagaa gtggcaattt caatttttct
    30181 acactgcgct ccatttcttt caacgcatct tctaaatgca agggctgcac cacaaaacca
    30241 caggtaaatt tatcaggatg ttcatgctgt actttcgcat taaaatcatt ttgccaacga
    30301 atggtatcgg atgtatcttg acgactccag ccattgcaat aaatttgcga taggttaagc
    30361 attacaccat ggtctatttg gtggcgttcc atccattcta atttgtcttg cagaaaaaag
    30421 ctaggatcgg taattggtcg tttccaatct ccttgacgca tgaatttctt gtcatcgtcg
    30481 atggaaaata aatgcttatc ttttaaaaat tgaggaacct cattgggttc tggcaaaata
    30541 tggccgtgac cgtttatttt catcgtacaa ttagttttag caagttttat tggttgtctt
    30601 ttttaaagat ttcactaatt cccttcccaa ttttttgcac cacttcaata cttttagtaa
    30661 ttggttcgga agaaatatcg tgataacctt cgtcggttaa ctcatcaatg tgacgcgagt
    30721 agataaccat gtaattattg ttttgcatgt atttttccat cttagaagga
//
LOCUS       DAOQVQ010000002        25397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_2275_length_25397_cov_4.381067, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000002 DAOQVQ010000000
VERSION     DAOQVQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 25397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25397
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_25397_cov_4.381067"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..2241)
                     /locus_tag="PKN22_00160"
     CDS             complement(292..2241)
                     /locus_tag="PKN22_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNS41141.1"
                     /translation="MGKHADTASKVSFATLLIALGIVYGDIGTSPLYTFKAIIGQDII
                     NEMLVLGGVSCVFWTLTLQTTVKYVILTLRADNEGEGGIFSLFSLVKRYGKFIIIPTM
                     IGASALLADGIITPAVTVTSSIEGLKMIEGLEHLHHVPIVLVIISAVFFAQRFGTQTI
                     GKAFGPIMLIWLTVLLVLGFNQIINSPSVLRALNPYYAYNLLVNYPQGFWILGAVFLC
                     TTGAEALYSDLGHCGRKNIYVSWVYVKICLVANYLGQAAWLLSNEGLFLRDQNPFFEI
                     MPQWFILPGIIIATLAAIVASQSLISGSFTLISEAINLNFWYRLRIKQPTEVKGQVYI
                     PSANNLLWIGCIFIVLFFQTSSKMEAAYGLAITITMMMTTYLLSFFLLYKLKWNKIIV
                     YTLVLIFSVIEISFFISNALKFGHGGYITILIGGVFFLVMYISFFGRKINDRYTQYVS
                     LGKYASKIAQLSNDESIPKFVTHLIYLSKSDKREIIEEQIIQSIFAKKPKRADVYWFF
                     HINRTNNPYTLDYDVSELVDDKIYKIIVNIGFRIKPRTETYFIQIINDMIENKEMSFH
                     LKNHGSTVYNPSIDFKFIVMERFLSVENEFALKEGLTLKAYFFLQKIAQNDEKAFGLD
                     KSDVLVEQIPLVYQPVGVVNLKRRI"
     gene            complement(2332..3009)
                     /locus_tag="PKN22_00165"
     CDS             complement(2332..3009)
                     /locus_tag="PKN22_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS41142.1"
                     /translation="MNKPVVLVIDDEPQIQKLLQINLENNGYKVHYAMTGKEGLLLAA
                     NQIPDVILLDLNLPDIHGHQLLLELREWYNKAIIILSVLNSEENIVKALDNGATDYLT
                     KPFRSGELLARIRSVIRRNSLDESSSVLQIGDIEIDLIAHVAKLNNEVLKLTATEYNL
                     LVLFAKNEGRVLTHQQLLKDVWGVGNQNDTQYLRVFVAQLRKKIEKDPNQPKHIITES
                     GVGYRFQ"
     gene            complement(3002..4075)
                     /locus_tag="PKN22_00170"
     CDS             complement(3002..4075)
                     /locus_tag="PKN22_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS41143.1"
                     /translation="MLQHLPFRTKQYIYSITLIVFLSLVCYLLEDYIHYYVVALVFLV
                     TVSILAIVYDIIPVLVSAILSGFVLNFFFINPVLHYKIHTYENVLLFFIYLLIALVSV
                     VIINKIRKQEKLLREREEKEKTIKLYDTLLNSLSHELRTPISTIIGSIDTLKENKETL
                     SAEYSNELLGGIEIAALRLNQQVENLLNMSRLETGSLTLKKDWCDINELIFMVIQKLS
                     NKSHNKEIVFHPNENLPYFKLDAGIIEQVLYVLIHNAIVYTPDFSRIEIEAFLQNDKL
                     AVKVRDNGVGIPEEFQESIFEKFYRLPNSKAGGSGLGLSIAKGFIDAHLGKIFLNSPS
                     NGGAEFCFTIPAETSYINSLKNE"
     gene            complement(4081..4707)
                     /locus_tag="PKN22_00175"
     CDS             complement(4081..4707)
                     /locus_tag="PKN22_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNS41144.1"
                     /translation="MRSTIVFVILVIVSSCTTIKSSQLKNGDLLFVSANNEGLSKAID
                     AVTQTKQSTHFSHIALLEKKNNQLFVMHAGTKNGSERISLQDFVLDEKNDKNTITVYR
                     LKRKYQHTIKNAIEVAKTWLGKPYNYSYVLSDEKLYCSDFVQRSFAKDTVFKLNPMTF
                     INPQTGKTDEMWIEFYKKQNIEVPEGKPGCNPNGMASSDKIKRMGILK"
     gene            5310..5999
                     /locus_tag="PKN22_00180"
     CDS             5310..5999
                     /locus_tag="PKN22_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS41145.1"
                     /translation="MTPKILLVEDDTSLGFVVADQLKRDGYKVSLCIDGAEGFKRFNE
                     EAFHLCIFDVMMPKMDGFTLARDVRKINKEVPILFLTAKSMTEDKIEGFKAGADDYLT
                     KPFSVEELQLRVVALLKRVNINVEEEQEKVIQIGNYQFDSENYTLSCGDSERTLTRKE
                     AHILRILTKFKNQVTPREMILNAVWGQDDYFVGRSLDVFITKLRKYFKDDERIAISNI
                     HGVGFKLEVIE"
     gene            5999..6667
                     /gene="tsaB"
                     /locus_tag="PKN22_00185"
     CDS             5999..6667
                     /gene="tsaB"
                     /locus_tag="PKN22_00185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNS41146.1"
                     /translation="MDVYILHLETATKVCSVAISKNGQLIALKESDDSEYLHGEVITL
                     FIEDVLKQAQLSIQQLSAVAITSGPGSYTGLRIGVSTAKGLCFALSIPLISVDALYSL
                     AYLAHQQHPHSTICAMIDARRMEVFSAIYDAQLNVLKPISADILDEATYQQFEPFIAI
                     GDGAPKMQELWKNRNIQFETAILCSAKGQITKAYEKFNHQEFEDVAYFEPFYLKDFVS
                     TAKK"
     gene            6664..7728
                     /locus_tag="PKN22_00190"
     CDS             6664..7728
                     /locus_tag="PKN22_00190"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNS41147.1"
                     /translation="MNLTQSILKKRPFIIAGPCSAETEEQVMLVAQQLAQTAKIDVLR
                     AGIWKPRTRPDSFEGVGVEALPWLVQAGKMTGLPTTTEVANKSHVEQALKAGIDILWI
                     GARTTVNPFAVQEIADALKGVSIPVMIKNPVNPDVELWIGAFERFAKAGISDLVAIHR
                     GFSVYKHEKYRNVPNWEIPIALKERMPDVPLICDPSHISGNSDLIFELSQSAMNLNFD
                     GLMIETHPQPAQAWSDAKQQITVDQLATILTNLVLRHNEIQGEVVGDMAKLRSMTADL
                     DERIFELLLERMKISEEVGKFKRDNNITILQHQHWTTIIANRLKCASEDGLSATFIRQ
                     IMDAIHQESIRHQEKVMNKS"
     gene            7725..8972
                     /gene="aroA"
                     /locus_tag="PKN22_00195"
     CDS             7725..8972
                     /gene="aroA"
                     /locus_tag="PKN22_00195"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNS41148.1"
                     /translation="MNYTVKNGTYKGKILIPSSKSDCQRAVLIAALGTNNSILKNVGS
                     SNDEQALLHTIQALGATVKMENKNTIIIAGNNQKPQLSTINCEESGLGLRLLTCIAAL
                     QNHEITMIGEGSLRKRHHLFFEKYLAQMGVQFSSNKGFLPFSVKGPLHAGNYTVDGGD
                     SSQYISGLLIAFSQVEGKTVLKVENCKSKAYIDMTLHTLKQFGINITILEDGVYAIEG
                     AQAVQPTTYTIEGDWSSASYWLVAAALGVNVQIDGLSLASKQADKALLQALLAANCRV
                     STSSDGITIDGTQRAAFEFDATHCPDLFPALTTLAVFTQGISKIKGVHRLANKESNRA
                     TALMSEFSKLGVEIFVENDGLVVVGGAPLVANLVDAHNDHRIAMTLAIASIVGQLELT
                     IEGAESVNKSYPDFWKHLEALQV"
     gene            complement(9112..9741)
                     /locus_tag="PKN22_00200"
     CDS             complement(9112..9741)
                     /locus_tag="PKN22_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41149.1"
                     /translation="MAKYRCLIFSLLILNSCDDKIIPTLKSKQIDYDSKTGILKIYED
                     SLNDLKEVILIPKQSNQKQIIRITDNFKISHLENRIRTFEDKNYLNTIIAFDRQGNFD
                     YFDSYYYISAPYKNKRGEVYVSCILDRSLFNDEIFAVVGEYDELFQLKTNGNIDTIRF
                     SNDREVFIPISNPKQGKNNLRFKIIDQKKTNNDTIRQRVIFVDKDFWIK"
     gene            complement(9962..10513)
                     /locus_tag="PKN22_00205"
     CDS             complement(9962..10513)
                     /locus_tag="PKN22_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HNS41150.1"
                     /translation="MIGNSKLSTALRETNHDLTNTFKDSYVFEFLNLPDPHSESDLQR
                     GLIRQMKNFILELGKDFLFIGEEYKLQVGNSDFFIDLLFYHRGLQCLVVFELKADKFK
                     PDHLGQLNFYLEALDRNVKKPNENPSIGILLCKDKDSEVVEYALSRSLSPTMVSEYKT
                     QLPDRKLLQQKLHELFDNETDDK"
     gene            complement(10565..10945)
                     /locus_tag="PKN22_00210"
     CDS             complement(10565..10945)
                     /locus_tag="PKN22_00210"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNS41151.1"
                     /translation="MDNRFTDIIQLIKQSRTNAIKAVNAELINLYWNIGEYISKKIEQ
                     SEWGDSVVTELANFIQTKEPEIKGFSDKNIWRMKQFYETYKHFPKLSPLVREISWSNN
                     LLIFSRCKTVEEMEFYLKMVKQEN"
     gene            complement(11180..11524)
                     /locus_tag="PKN22_00215"
     CDS             complement(11180..11524)
                     /locus_tag="PKN22_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41152.1"
                     /translation="MQFIRKYWVAVICVIIFFALLFNLLNEHKKEKLLQKSNSVIGLL
                     QKEDHSSLKFNDGIFIFFVKNKNYIIRHKGDFSHLKKGDTVLIEYAIEDPSVARVVDK
                     YYMQKYKHLKEK"
     gene            complement(11560..11961)
                     /locus_tag="PKN22_00220"
     CDS             complement(11560..11961)
                     /locus_tag="PKN22_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS41153.1"
                     /translation="MKYAEIINKKQYYEYSQRHLELGKILGAGKGNDDIESEYYILDL
                     ILEDYTRKVKSPFNDLTPVDLLKALMKEHGYTAYKLSKELEIPQSILSDILNYKRGFS
                     KELIRKFATKFKMSPASFFKEYTLVKASKIV"
     gene            complement(11995..12315)
                     /locus_tag="PKN22_00225"
     CDS             complement(11995..12315)
                     /locus_tag="PKN22_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNS41154.1"
                     /translation="MKVRVITEISIWEYCATNARAIPSFHIFLEKLKNCDWNDLNDMK
                     ADFPKLSIIGNCENNRIVFDIGGNNYRMICDYNFYKNCCLYVAFIGTHAEYDKVNACT
                     VQMF"
     gene            complement(12458..14704)
                     /locus_tag="PKN22_00230"
     CDS             complement(12458..14704)
                     /locus_tag="PKN22_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNS41155.1"
                     /translation="MPRLYLIALFCCVFSISWGQNMLSKYLKVADEKYKKGDYYYALE
                     LYSKAMEIDSNTIDILWKVAETHRAYKDYRKAEYYYAKVYERELGKIYPSSLLQLGLM
                     QKQNGKYDQAIETFKKAKKKYKDKKKDYLYLKSKQELESTLWAKSREDIVEKGDFVRL
                     PETVNTKNSEFGHNFVLNELVFSSLRADSISANEEVYEKHYTTSLFKSKFEEGRFDNS
                     IKWQELTSNNENTGNAALSLDGKRLYFSSCKDVDGISYQCKLVVAKFVNGKWGMVDTL
                     GEIINAPNANTTMPAIGELDGKEVLFFCSDNSQETKGGLDLFYTTITNGNQFGRVKSI
                     KSLNTIDNEVTPFWDKNQQRLYFASTWHNGFGGFDVFYSQYKDGQFEEPQNVGQPINS
                     PANDLYYFNRNDTSYVTSNRIGVLYSKNITCCSDIFALYPPMKENPPTIKETLEELSK
                     RLPVTLYFHNDIPDPRSWDTVTKLSYMTTYNDYTAMMPRYQKEYAVGLDKDKAEEASE
                     DIENFFTQYVDQGVKDLVLFRDLLLEELQKGARVKVVVRGFASPLAKTEYNVNLTKRR
                     ISSLINHLREFNGGVFIPYLDGTAANNGRVVFEQVPFGEYNANQLISDNPNDTKNSVY
                     SRAAALERKIEIQSVTYLDKQAVFPLVVQKPVFNAGVLPSGQLIQAEFVVENTSDSLV
                     ELQPNLTPNSFTTLTMAQTTIPPKGKITIGMQLNTKGMIGHTVKTVEIPVNNQTEQLK
                     LVVTMELK"
     gene            complement(14686..15687)
                     /locus_tag="PKN22_00235"
     CDS             complement(14686..15687)
                     /locus_tag="PKN22_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS41156.1"
                     /translation="MKNICALIVTLLLTQIAGAQDIHWSQFNDNPIFQNPGNTGNFNG
                     DIRFVGNFRDQWRAVSVPYQTISVSADGKLYNYRNLGIGGMFFHDVTGDGRLRTIELQ
                     ANVAYLLKLTKDSTHTITPGINLGMNHRQVNWNAFQFGNQYNGQYYDANLPTNELYQS
                     DRKTNFSVGVGAVYRYLIGKRKTLEAGVGVFNLNRPNQGFFNEKIHRDIRTSVFAKFY
                     YPIGLDWDIIPSFQFSFQGKYREFIVGSSVKYTIVNRLAKYAAVYGGIWYRAVDAAYL
                     TVGMDYQQWFVGLSYDMNLSTLTKASRVRGGFEVAVRYILFRFKPKKIVHRICPDYI"
     gene            complement(15762..17027)
                     /locus_tag="PKN22_00240"
     CDS             complement(15762..17027)
                     /locus_tag="PKN22_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41157.1"
                     /translation="MEQQGSVEKRPKKRFWKWIKITYFIGIHILAVFAFVMIGIAVAA
                     KFKWTNDQGKVDVNNRYFAQMANKYNQGFKLDSLNFESEERQMYQRVGLLAQHYPYNA
                     KTILAAYEKSGDLMVALRMLDATNLLLKNDKAYQKKVREIEAKKDDNTLSLYPWANYT
                     EWKDFCKYVKADKKAIDSVAKITGVESRLIVMCLVGEQIRMFNSSREKLKNQVYKYNH
                     IFLPKNRGYGVTGILESTALRIENTLFTPNSPFYPGDYYQKCINLKDTFPGSVIDSIE
                     AHKFPTIQRLIQGGTHYYSYLYTALLLRQYQSHWEKAGYTLAYRPEILASLFNLGYQK
                     SKPGPSPQVGGSNFKVGDHDYTFGGLCFEFYYSGELLDLFPLTSRPFIPTEELERTIV
                     FPEVEEETPIETPTTSGGGLQMMHQIDSL"
     gene            17256..19313
                     /gene="metG"
                     /locus_tag="PKN22_00245"
     CDS             17256..19313
                     /gene="metG"
                     /locus_tag="PKN22_00245"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNS41158.1"
                     /translation="MQKDKFTVTAALPYANGPLHLGHVAGVYLPADIFVRFLRMNNHD
                     VAFICGSDENGAAITLRAKKEGITPQEIVDKYHTIIGNSFKDFDISFDIFHRTSLPLH
                     HETSQEFFKVLHANGSFTQETSEQYYDEEFKQFLADRYITGECPKCHSANAYGDQCEK
                     CGSALSPLELINPKSTLSGKTPVLKQTSHWYLPMQRHEGWLKEWIQKGTLDGVQQHEP
                     KKWRNQVLGQCMSWIDGGLHSRAMTRDLDWGVKVPLPDSEGKVLYVWLDAPIGYISAT
                     KQWAMDNGKNWEEYWKGDRKLVHFIGKDNIVFHAIIFPILLKAHGDLILPDNVPAYEF
                     LNLEGEKFSTSRNWAVWLHEYIASYPERIDELKYVLSAIAPESKDSEFTWKEYQARVN
                     NELADILGNFVNRALVLTQKYYGGAVPKLGELTDADQQVLAEMKAIPAKISQLIYDYK
                     LREAQAEAMNLARLGNKYLAENEPWKLVKENPTRVETIMNIALQITANLSIVLQPFLP
                     KTAQKLSKFLNFETKSWENAGKMDLLAEGHATNQPEILFTKVEDAMVEAELEKLKNAN
                     PVAASNFNPQKDNATFDDFTKMDIRLGTILEAEKVEKADKLLKLLVDTGIDKRTIVSG
                     IAAHYQPEDVVGKTVAVLINLEPRKIRGVESQGMILMAENEKGELSFMVPEKGFGAGF
                     EIR"
     gene            complement(19310..20857)
                     /locus_tag="PKN22_00250"
     CDS             complement(19310..20857)
                     /locus_tag="PKN22_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNS41159.1"
                     /translation="MQQGIFSQRIHSLIQFFSFALIAIGMPTTKVLMSFGLVFAIANW
                     VLEGQFVQKWQIIRSNRTLMLLIGFYVLFIVGVSWSWDTMQGLKDLKSRLPFVFLPLI
                     VATNPQQQVKYYYYLLYLFLATLLATSIFNVVYFQLYLKNNIYEDIRGMSRFASHIRY
                     GLLIVLGICSACWLQWQAKKWNWGWTALAIWFTFYTFYSAILSSILALVLVVVVLLFY
                     FLAQWKKSIAYLFAGSVVVGLGVLLFLVTNIQHEKIDCATLPAVTENGVPYNHECDKF
                     SEINGKTIFAYYSVIELFHEWQKVSKMDLMRSDKRGGMLRVTCARYMTAMGLTKDKKG
                     FQQLSAQDIRNIEDGYTYPNERNELLMPRLYGIKYQLLNNQDPNGHSLLQRLEHWKTS
                     LYIIQHNWLLGVGTGGNQKAFDAAYEATNSPLSPENRVRSHNLFLTYGVSYGILGALF
                     FILLMGALLIKAWKSNNLLNISIVVVLISSFLTEDTVETQLGVTLFGFFIALLFVNWN
                     TTENKFN"
     gene            complement(20886..22535)
                     /locus_tag="PKN22_00255"
     CDS             complement(20886..22535)
                     /locus_tag="PKN22_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNS41160.1"
                     /translation="MKNNTIQSFALSAIAVFIISIQFSWAQSERFEIIKNMELIDEIY
                     ENLDKYYVESPKIGKMSVAGISAMLEDLDPYTVFYPESDIEDYRLMTTGQYGGIGAII
                     RKIGDYVVIAEPYEGSPAATAGIRAGDKILAIDGRSMQKKNTDEVSSNLKGPKGTTIT
                     VEVERPNGAKETVKITRDEIKVPDIPYAGMLNNEVGYIKLTSFTQTASSEVKKNFLSL
                     KEKGMKKLVFDLRGNGGGLLMEAVHIVNMFVPKGQLVVQTKGRLVEENREYKTVNEPL
                     DLNIPVVVLVDGGSASASEIVSGSLQDLDRAVVVGETSFGKGLVQRTLDLSYGAKMKL
                     TIAKYYTPSGRCVQRLEYYDRNEGAKPEDVPDSLLHVFKTKNGRDVIDGRGIEPDMKV
                     EAPELSRLALMIYANNVLFNYVTDYVKTHPSIAPAEQFRLSDEDYEAFKKYAIQDTFT
                     YSTASEEQLMKMQKTIEKENLMDDAKAEYEALMAKITPSKERDLVKFEAQIRQLIENE
                     IVSRYYFQQGRVQNSFNHDEVLKKGIEVLNNTQQYNTILKK"
     gene            complement(22525..22929)
                     /locus_tag="PKN22_00260"
     CDS             complement(22525..22929)
                     /locus_tag="PKN22_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNS41161.1"
                     /translation="MNQTFGKEYKLCSKKTIDALFQEGQRLNVYPLHLTYQLIDQPNS
                     KAPFQVVTSVPKRQFKRAHDRNYIKRLLRESLRLNKTILEDFLANENLKNKQLVLFLV
                     YRDNKQPEYQFLFKKVNKLLLTLVATLEKNEK"
     gene            23029..23901
                     /locus_tag="PKN22_00265"
     CDS             23029..23901
                     /locus_tag="PKN22_00265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS41162.1"
                     /translation="MYFDKTYAMKHQQHPFGSIFESVMYSLLLLVVLWLVQWVQSVFH
                     YDFYKLGMLPRTWSGMKGVLFMPLLHSRHDIHHILNNSFALLVLLTMLIYYYRAIAFK
                     VFVLLWLMTGLLVWAYAQNKGVYHIGISGVIYGLVGFLFTSGVLRKYLPLQAISLLVI
                     FAYGNLIWGIFPVQASVSWEGHFMGLVAGLLLAFAFRSKAVQAPKYQYEIEKELGIEP
                     PDLEAIWNEKQAQFHQQSTAEESPTSMLPIKIVYLYKTDTEQEQASEQGNNTNQSTTW
                     TKNDGETQQLPNQH"
     gene            complement(23858..24343)
                     /locus_tag="PKN22_00270"
     CDS             complement(23858..24343)
                     /locus_tag="PKN22_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNS41163.1"
                     /translation="MKFLYILRHAQPHSTAPSGNDFDRQLNDKGKQQVQLLAAFLEQH
                     PISVPFECVVSTAKRTQQTFAGIQTVLEKNNCYHGAAFYDQLYHASQSDLLQFLWNYT
                     SENNILLVGHNNGISDMVSYFIGKNVHLEPADFICLAFDVDYWKEISADLATVVFHRH
                     F"
     gene            24470..25066
                     /locus_tag="PKN22_00275"
     CDS             24470..25066
                     /locus_tag="PKN22_00275"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNS41164.1"
                     /translation="MSFELPKLPYAYDALEPHFDARTMEIHHTKHHQAYTTNLNNAIA
                     GTDLEGKTIEEILKVCADKPAVRNNGGGFYNHNLFWTVLSPNGGGQPTGELAAAIDQS
                     FGSFDAFKEEFSKAAATRFGSGWAWLNVDGGKLTITSSPNQDNPIIEGSSATPILGLD
                     VWEHAYYLHYQNRRPDYISAFFNIINWEEVTKRYNAAK"
BASE COUNT         7900 a         4688 c         4543 g         8266 t
ORIGIN      
        1 ccttcatcag aatagtttcc tcctgctttt gtcgcccatt caaaatgagg actgtcttgg
       61 ctaaaaatgg aggtggagaa aagactaaaa aagcctataa gtgctgttaa tgctattttt
      121 aaagataatt ttttcatagt tgcttgttta aggttacatt tgttaaatgt aagaaaattc
      181 attcataaaa acacccaatg ttggatattt ttacacattt caaacaaaca ttgcttggtt
      241 gcttaaacag cactcacctt gtttactatt tttattagtt gacttcccct cctaaattct
      301 tcgtttcaaa ttaacaacac caacaggctg gtaaacaagc ggaatttgct ccacaagcac
      361 atcgctttta tccaaaccaa aggctttttc gtcgttttgt gctatttttt gtaaaaagaa
      421 ataggctttc agtgttaggc cttcttttaa tgcaaattcg ttttcaacgg ataaaaagcg
      481 ctccatcaca atgaatttaa aatcaatgct gggattataa acggttgaac catgattttt
      541 taaatgaaaa ctcatttctt tgttttcaat catgtcattt atgatctgaa tgaaataggt
      601 ttcagtgcgt ggcttgatgc ggaaaccaat gttaacaatg attttgtaaa ttttatcgtc
      661 caccaattca gatacatcgt agtctaaagt gtagggattg ttggttcggt tgatgtggaa
      721 aaaccaataa acatccgctc ttttgggttt tttagcaaaa atagattgaa tgatttgttc
      781 ttctataatt tctcgtttat cggatttgga aagatatata agatgtgtca caaatttggg
      841 gatggattca tcgttgctta attgcgcaat tttagaagca tatttaccta aactcacata
      901 ttgcgtataa cgatcgttaa tttttcgtcc aaagaaactg atgtacatca ctaagaagaa
      961 aactcctcca atcaaaatgg taatgtaacc tccgtgtcca aattttaatg cgttggaaat
     1021 gaaaaacgat atttcgatca ctgaaaaaat aagcactaat gtgtacacaa taattttgtt
     1081 ccactttaat ttataaagga ggaagaagga gagcaaatag gttgtcatca tcattgtaat
     1141 ggtaattgcc aacccataag cggcttccat tttagaagat gtttgaaaga acaaaacgat
     1201 gaaaatacac ccaatccaaa gtaaattgtt ggcacttgga atataaacct gacctttaac
     1261 ttctgtgggt tgtttaatgc gtaaacggta ccaaaaattt aagttgatag cctcactgat
     1321 taatgtaaaa gaaccactaa ttaaagattg agaagcaaca atggctgcaa gtgttgcaat
     1381 aatgatacct ggcaaaataa accattgcgg catgatttca aaaaatgggt tttggtctct
     1441 taaaaacaat ccttcgttgc taagcaacca tgccgcttga cctaagtagt tggctaccaa
     1501 gcaaatttta acataaaccc aactcacata aatgtttttt ctgccacagt gacccaaatc
     1561 agaatacaac gcttcggctc cggttgtaca taaaaatact gctcccaaaa tccaaaaacc
     1621 ttgtggataa ttcaccaata aattataggc atagtatgga tttaaagctc taagtactga
     1681 aggagaattg ataatttgat tgaaccccaa caccaataaa acagtaagcc agatcaacat
     1741 gatgggtcca aatgcttttc caatggtttg tgtaccaaag cgttgagcaa aaaacaccgc
     1801 cgaaataatt accaaaacaa ttggaacgtg gtgcaaatgc tccaatcctt caatcatttt
     1861 taaaccttca atggaggagg taaccgtaac ggctggagta ataattccat cggctaaaag
     1921 cgcagaggca ccaatcattg ttgggataat gatgaattta ccatatcttt taactaatga
     1981 aaataaagaa aaaattcctc cttcgccttc attgtcggca cgcaatgtaa ggatgacata
     2041 ttttacagtt gtttgcaaag tcaaggtcca aaacacacac gatacacctc ccaaaaccaa
     2101 catttcgttg atgatatcct gaccaataat tgctttgaag gtatagagtg gactggtacc
     2161 aatgtcacca taaacaattc ccaatgcaat taacaaggtt gcaaaactta ctttagatgc
     2221 ggtatcggca tgttttccca tgaatcatta ttttttgagc aacaaaattg gtgcagattc
     2281 aataagaaaa aaataaagaa gtgaatcgtt ggtataaaga ttttataaag attattgaaa
     2341 acgatagcca acaccacttt cggtgatgat gtgtttgggt tgattgggat ctttttctat
     2401 cttttttcta agttgcgcaa caaaaacacg taaatattgc gtatcgtttt gattgcccac
     2461 gccccaaaca tctttcaata attgttggtg ggtgagaacg cgcccttcat tttttgcaaa
     2521 caacaccaat aaattatatt ctgtagccgt taattttaag acctcgttgt tgagtttggc
     2581 aacatgtgca attaaatcaa tttcgatgtc gccaatttgc aaaacagatg aactttcatc
     2641 caaactgttt ctgcgtatga cagagcgaat gcgcgctaat aattcccccg atctaaatgg
     2701 ttttgttaaa taatccgttg ctccgttgtc caatgctttt acaatgtttt cttctgaatt
     2761 taacaccgaa agaataatga ttgccttgtt gtaccattct ctcaattcca ataaaagctg
     2821 atgcccatga atatcaggaa ggtttaagtc taaaagaata acatccggga tttgattggc
     2881 tgccaatagc aatccttctt tacccgtcat ggcatagtgc accttgtaac cgttgttttc
     2941 taagttgatt tgcaacaact tttgaatttg tggttcgtca tcaattacta atacaactgg
     3001 cttattcatt ttttaaacta ttgatataag aagtttctgc tgggattgta aaacaaaact
     3061 cagcacctcc attggagggt gaatttaaga aaattttgcc tagatgtgca tcaataaatc
     3121 cttttgcaat ggataaacct aaaccagaac ctccggcttt gctattggga aggcgataaa
     3181 acttttcaaa aatagattct tgaaattctt ctggtattcc aacaccgtta tcgcgcactt
     3241 ttactgctaa tttatcgttt tgcaaaaatg cttcaatttc aattctagaa aaatcggggg
     3301 tgtaaacaat ggcattgtga attaatacat acaacacttg ttcaattatt ccagcatcta
     3361 atttaaaata aggtaaattc tcgttgggat ggaaaactat ttctttgttg tggcttttat
     3421 tggatagttt ttgaatgacc ataaaaatca actcattaat atcgcaccaa tcttttttga
     3481 gggttaaact acctgtttca aggcgactca tgttaagtag gttttcaact tgttggttga
     3541 ggcgcaaggc agcaatttca attccaccca aaagttcatt gctatattct gcagatagtg
     3601 tttctttatt ttctttcaac gtatcaatac ttccaatgat ggttgaaatg ggggtgcgca
     3661 actcatgcga tagtgaattg agcaaagtgt cataaagttt tatcgttttt tctttttcct
     3721 cgcgttcacg caatagtttt tcttgttttc tgattttatt gataataacc acactcacca
     3781 aggcaattaa caagtagatg aaaaacagca atacattttc ataggtatgt attttgtagt
     3841 gcaacaccgg attaatgaaa aagaaattga gtacaaaacc actcaaaatg gctgatacga
     3901 gaacaggaat aatgtcgtaa acaatagcta aaatggatac cgttaccaaa aacaccaagg
     3961 caacaacata gtaatggata taatcttcca ataaataaca aaccaaggaa agaaatacga
     4021 ttaacgtaat actataaatg tattgcttgg ttctaaatgg aagatgttgc aacatgttgt
     4081 ttattttaaa atacccattc gtttaatttt gtcagaactt gccattccgt ttggattgca
     4141 acctggtttt ccttctggaa cttcaatgtt ttgctttttg taaaactcta tccacatttc
     4201 atccgttttt cctgtttgag gattgatgaa ggtcattgga tttaacttaa aaaccgtatc
     4261 cttggcaaaa ctacgttgta caaaatctga acagtagagt ttttcatccg ataaaacgta
     4321 ggaataatta tagggtttcc ccaaccatgt tttggctact tcaattgcat ttttgatggt
     4381 gtgttgatac tttcttttta aacgatatac agtgatggtg tttttgtcgt ttttttcatc
     4441 taacacaaaa tcttgcaagg aaatgcgttc tgacccattt ttagtgcctg catgcattac
     4501 aaacagttga ttgttttttt tttctaataa agcaatatgg ctaaaatggg tggattgttt
     4561 ggtttgtgtt accgcatcaa ttgctttgga tagtccttca ttatttgccg aaacaaagag
     4621 taaatcacca ttttttagtt gactactttt gattgtggtg caagaagaaa ctataacaag
     4681 aatgacaaaa acaatagttg aacgcatctt tatatattta gagttacaaa tgtaagaaaa
     4741 tctaatgttg aaggctttta tttccgacta gattaaatca attttcttgc aaacgacatt
     4801 cttaagttca tttttgaata caaactactt ttaagagagt gttgaaaact atcaaaatcc
     4861 atttttctac gaatatttca cccttttttg aaagaatttt catgttttcg tcgaaaataa
     4921 tacgtctaaa aaattgtcat ccttggaatc ctaaaaatga gttttgaatg ttaaaatatg
     4981 aacatttaag ttaaaaatac aagttaaacc gtttagtagt aggcaatgct ggatgtttaa
     5041 gcttggaaaa aaagcgttta ttttaccctt cctatttttt gcacttttcg taggtgaccg
     5101 caaaacatgt tttttgacct gaattttgac ttatctttgc atcaagtagt agtagtagta
     5161 ggttaggttg attagtgtgg caagggcttt agattttctg aagctcttgt tttttttata
     5221 taaactttgc ttcttcaatt ttttaaaaaa atcaattttc ttacggtttt caatttttaa
     5281 tctattaact ttacataaag tattgagtta tgactccaaa aattttgtta gttgaagacg
     5341 ataccagtct tggttttgtg gtggcagatc aattaaaaag agatggttac aaagtttctt
     5401 tgtgtattga tggtgctgaa ggatttaaac gtttcaatga agaagctttt cacttgtgca
     5461 tatttgatgt gatgatgccc aaaatggatg ggtttacact ggcacgcgat gttcgaaaaa
     5521 ttaataagga ggttcctatt ttatttttga cggcaaaatc aatgacagaa gataaaattg
     5581 aaggatttaa agccggagca gatgattacc tgaccaaacc attttctgtt gaagaacttc
     5641 aattgcgagt tgtggcgttg ctaaaacgtg ttaacatcaa tgtggaagag gaacaagaaa
     5701 aggttattca aattggaaat taccagtttg attctgaaaa ctacaccttg tcttgtggtg
     5761 atagtgaacg aactctaacc cgaaaagaag ctcatatact tcgtatttta acaaaattca
     5821 aaaatcaagt cacgccacgt gaaatgattt taaatgctgt ttggggacaa gacgattatt
     5881 ttgtaggtag aagtttggat gtgttcatta ctaaattgcg caagtatttt aaagacgatg
     5941 aacgtattgc catcagtaac attcacggtg ttggattcaa attggaagtg atagaataat
     6001 ggatgtttac attttgcatt tggaaacagc aacaaaagtc tgttcggttg ctatttctaa
     6061 aaacggtcaa cttatcgccc tcaaagaaag tgacgacagt gagtatttgc atggagaagt
     6121 catcacgctt tttattgagg atgtactaaa acaagcccaa ctttccattc agcaattgag
     6181 tgcagttgcc attacttctg gtccgggttc ctacacagga ttgcgcattg gtgtttcaac
     6241 cgccaaagga ttgtgttttg cactttctat tcctttaatt tcggttgatg ctttgtatag
     6301 tctagcatat ttagcacacc aacaacatcc ccattctacc atttgtgcca tgattgatgc
     6361 acgccgcatg gaagtgtttt ctgccattta tgatgcccaa ttgaatgtgc taaaaccaat
     6421 ttcggctgat attctggatg aagcaaccta ccaacaattt gaaccattta tagccattgg
     6481 cgatggagct ccaaaaatgc aagaattatg gaaaaatcgc aacattcaat ttgaaactgc
     6541 tattttgtgc tctgcaaaag gtcaaattac aaaagcttat gaaaaattta accaccaaga
     6601 atttgaagat gtagcttatt ttgagccatt ttatttaaaa gattttgtaa gcaccgccaa
     6661 aaaatgaatt taacacaatc catattaaaa aaacgaccat ttatcatcgc cggaccgtgt
     6721 agtgccgaga cagaagagca agtaatgtta gttgctcaac aattggcaca aacagcaaaa
     6781 attgatgtgt tgcgcgcagg tatttggaag ccacgcacgc gccccgattc ttttgaaggt
     6841 gttggcgtag aggcacttcc gtggttggtt caagctggaa aaatgacagg attgccaact
     6901 acaacagaag tggccaataa atcgcatgtt gaacaagcac taaaagccgg aatagacatt
     6961 ctttggattg gtgctcgcac aacagtaaat ccttttgctg tacaagaaat tgctgatgca
     7021 ctaaaaggtg tttctattcc tgtgatgatt aaaaaccctg taaatccaga tgtggaatta
     7081 tggattggtg cttttgagcg ttttgccaaa gctggaattt ctgatttagt agccattcac
     7141 cgcggatttt cggtctataa acacgaaaaa tacagaaatg tgcccaattg ggaaattcct
     7201 attgcattga aagaacgtat gcccgatgtg ccactaattt gcgaccctag ccatatttct
     7261 ggaaatagcg atttgatttt tgaactgtcg caatcggcaa tgaacctcaa ttttgatggt
     7321 ttgatgatag aaacgcatcc acaacctgca caagcgtggt cggatgcaaa acaacaaatt
     7381 acggtagatc aacttgcaac catattaact aacttggtat tgcgtcacaa cgaaattcaa
     7441 ggtgaagtgg ttggagatat ggcaaaattg cgctccatga cagctgattt agacgaacgt
     7501 atttttgaat tgctcttgga acgtatgaaa attagcgaag aagtgggtaa gtttaaacgc
     7561 gataacaaca tcaccatttt gcagcatcag cattggacaa ctattattgc caaccgacta
     7621 aaatgtgcaa gtgaagatgg actttcggca acatttattc gccaaattat ggatgccatt
     7681 caccaagaat caatccgtca tcaagaaaaa gtgatgaata agtcatgaat tacacagtaa
     7741 aaaacggcac ctacaagggt aaaattttga taccatcgtc aaaaagcgat tgccaacgtg
     7801 cagtgttaat tgccgcatta ggaaccaaca attctattct aaaaaatgta ggttcaagca
     7861 acgacgaaca agctctgttg cacaccatac aagcacttgg agccacagta aaaatggaga
     7921 ataaaaatac cataattatt gctggaaata atcaaaaacc gcaactttca actataaact
     7981 gcgaagaaag tggtttagga ctgcgtttat tgacctgtat agcagctttg caaaatcatg
     8041 aaataacaat gataggagag gggtctttgc gcaaacgtca tcatttattt tttgaaaaat
     8101 acctcgctca aatgggggtt caattttcat ccaacaaggg gtttttacca ttttctgtaa
     8161 aaggtccatt gcatgcgggc aactacactg ttgatggtgg agatagctcg caatacattt
     8221 ctggtttact gattgcattt tcgcaagtgg aaggaaaaac ggtgctaaaa gtggaaaatt
     8281 gcaaaagcaa agcatacatc gacatgacgc tccacacgtt gaaacaattt ggcattaaca
     8341 ttactatttt ggaagacggt gtgtatgcaa ttgaaggtgc acaagcagtt caaccaacca
     8401 cctacaccat tgaaggcgat tggagttcgg cttcgtattg gttggtggca gctgcgttgg
     8461 gggtgaatgt tcaaattgat gggttatcgc ttgcatccaa acaagcagat aaggcacttt
     8521 tacaagcatt gctggctgca aattgccgtg ttagcacttc ttctgatggt attacaatcg
     8581 atggaacaca aagagcagca tttgaatttg atgctacgca ttgtcctgat ttatttccag
     8641 cattgaccac gcttgctgtt tttacccaag gaatttcaaa aataaagggt gttcatcgcc
     8701 ttgctaacaa agaaagcaac cgcgcaacag cattaatgag tgaatttagc aaattaggag
     8761 tagaaatttt tgtagaaaac gatgggctag ttgttgtagg gggcgcacca ttggttgcaa
     8821 atttagttga tgcccacaac gaccatcgta ttgcgatgac actggccatt gcttccattg
     8881 ttggtcagct tgagctaaca atagaaggtg cagaaagcgt caataaaagc tatcccgatt
     8941 tttggaagca tttggaagcc ttgcaggttt aattttttta atgttgagga tgcttctttt
     9001 tgcgatttgc ttgttttctt gcaagtgagc ttattagatc tgctttattc tttttatttg
     9061 ttttattaat cagaccaata ttgaaaaacc tatacatatt gatcatgctt tttattttat
     9121 ccaaaaatcc ttatctacaa aaatgactct ctgacgaatt gtatcattat ttgttttttt
     9181 ttggtcaata attttaaatc gtaaattatt ttttccctgc ttaggattgg atattggaat
     9241 aaatacttct ctatcattag aaaaacgaat agtatcaata ttcccatttg tttttaattg
     9301 aaataattca tcatattccc ccacaacagc aaaaatttcg tcattaaaaa gtgatctgtc
     9361 aagaatacaa ctaacatata cctcccctct tttgttttta taaggggcag atatataata
     9421 gtaagaatcg aaataatcaa aattaccttg tctatcaaat gcaataatcg tattcaaata
     9481 atttttatct tcaaatgttc taattctatt ttcaagatga gatattttaa aattatcagt
     9541 tatccgaatt atttgttttt gattcgactg tttaggtata agaatcactt cttttaaatc
     9601 atttaaagaa tcttcataga ttttaagtat tcctgttttt gaatcataat caatttgttt
     9661 tgattttaaa gtggggatta ttttatcatc acacgaattc aaaatcaaca aagaaaaaat
     9721 caaacaacga tattttgcca atatatttac cattttttct ctacgtcaat taaagtgttt
     9781 tttctttttt tttataaaat tcaattgcgt tcactcttca aatttaatga aaattgttag
     9841 gcggactgtg ctaactgctt tctactctta aaactactat tttttagata aggagcattt
     9901 tgtgggagaa acgaagcatt taaccaccac ttttgtcaaa ccgatgtaag cagtagtggc
     9961 tttatttgtc atctgtctca ttgtcaaaaa gctcgtgtaa tttttgttgt aaaagttttc
    10021 tgtcaggtag ttgtgtttta tattccgaaa ccattgtcgg tgaaaggctt ctgcttaaag
    10081 catactcaac aacctcgctg tctttgtctt tgcagagtaa aatccctatg ctcggattct
    10141 catttggttt cttaacattt ctgtctaacg cttccaaata aaagttaagc tgtcctaagt
    10201 ggtcgggttt gaatttatct gcttttagtt caaataccac taaacattga agtccacggt
    10261 ggtagaacag caagtcaatg aaaaagtcgc tgttgccaac ttgcaactta tattcttctc
    10321 cgatgaacaa gaagtcttta ccaagttcaa ggatgaagtt tttcatttgc cttatcagtc
    10381 cacgctgtaa atcgctctca ctatgtggat caggtaagtt caaaaattca aatacatagc
    10441 tgtctttaaa agtattggtt aggtcgtgat tcgtttctct caacgctgtc gagagttttg
    10501 agttgccaat cattgttcgc tcaaaaagac ttgcagaaat ttggcggtca agttcacgtt
    10561 tactttaatt ttcttgttta accattttga ggtaaaattc catttcttca acagttttac
    10621 atctggaaaa aatcaacaga ttgtttgacc aactaatttc tctcaccagt ggtgagagtt
    10681 ttgggaagtg cttgtaagtc tcgtagaact gtttcattct ccagatattt ttatcggaga
    10741 agccttttat ttctggttct tttgtctgta tgaaattggc taattcagtc accacagagt
    10801 cgccccattc tgattgttca attttcttgc tgatatattc acctatgttc caatagaggt
    10861 ttattagttc agcatttacg gcttttattg cattagtccg agattgttta atgagctgaa
    10921 ttatgtccgt aaaacgatta tccattatta atttctatat gctacaaatt tatgtttttg
    10981 gtatccgttg caatggtggc ggcatcggtg atggagaaac aaatttcctt ccacccactg
    11041 tttatttgtt tgtgtttttt ggttgccact ctcataagca attgccgttc ttagcttaaa
    11101 gttagtgttt ttttgagata aggagtgttt tgggcggtga ttgattaatt ttttttgtat
    11161 ttgtagttta tgttactttc tacttttctt ttaaatgttt gtatttctgc atataatact
    11221 tatcaacaac ccttgcaaca gatggatctt ctattgcata ttctattaaa acggtgtcgc
    11281 ctttttttaa atgagagaaa tcacctttat gtctaatgat ataatttttg ttttttacaa
    11341 aaaatataaa aataccatca ttaaatttaa gactagaatg atcttctttc tgtaataagc
    11401 ctattacaga atttgatttt tgtaataatt tttctttttt atgttcattt aatagattga
    11461 acaaaagagc aaaaaatatt attacacata ttactgctac ccaatatttt cgtataaatt
    11521 gcattcaatt ttttatatta aatagtaatc taaatgtgtt taaacgattt tagatgcttt
    11581 aaccaatgtg tattctttaa aaaaagatgc tggactcatc ttaaatttag tagcaaattt
    11641 tcgaattaat tccttgctaa aacctctctt ataattgaga atatctgaaa gaattgattg
    11701 gggaatttcc aactcttttg aaagtttata tgcggtatag ccatgttctt tcatcaatgc
    11761 tttgagtaaa tcaacaggtg ttaaatcatt aaaaggactt ttaacttttc gtgtgtaatc
    11821 ctctaaaatt aaatcaagaa tataatattc gctttcaata tcatcattcc cttttcctgc
    11881 acctaaaatt tttcctaatt ccaagtgtcg ctggctgtac tcatagtact gcttcttgtt
    11941 tataatttct gcgtatttca tatttctttc ttttaaactt attatggcgt aaaactaaaa
    12001 catttgaact gtgcaggcat ttactttatc atattcagca tgtgttccaa taaaagcaac
    12061 atacaaacaa caatttttat aaaaattata atcgcaaatc atccgatagt tattgcctcc
    12121 aatatcaaat acaattctgt tgttttcaca attaccaatt atacttaact taggaaaatc
    12181 tgccttcata tcattcaaat cattccaatc acaattttta agcttttcta aaaatatgtg
    12241 gaaacttgga attgctctcg cgttagtggc gcaatattcc catattgata tttcggttat
    12301 taccctaact ttcatttagt ttttacttgt tcgttttaca aatatatgaa atacttatca
    12361 aagtgcgaag tattttttaa ttgttttttc atttttctga aatgattgtt aatgaaaggg
    12421 atttataatt tcatttcaaa ataaccatac tcaacaatca cttcagctcc attgttacca
    12481 ccaacttgag ttgctctgtt tggttattga ccggaatttc aacggtttta acggtatggc
    12541 caatcattcc tttggtgttg agttgcatgc caatggtgat ttttcctttg ggaggaatgg
    12601 ttgtttgtgc catcgttaaa gtggtgaaac tgttgggagt taaattaggt tgcaattcta
    12661 caagactatc cgatgtgttt tcaactacaa attcggcttg tatgagttgt cccgacggca
    12721 aaacacctgc attaaacacg ggtttttgaa caaccaatgg aaaaacagct tgcttgtcta
    12781 aataggttac actttgaatt tcaatttttc gctccaatgc tgcagcacgc gaataaaccg
    12841 aattttttgt atcgttggga ttatcactaa tcaactggtt ggcattgtat tctccaaaag
    12901 gaacttgctc aaaaactaca cgaccattat tggcagctgt tccatctaaa tacggaataa
    12961 aaacacctcc attaaactca cgcaaatgat tgattaacga tgaaatccta cgttttgtga
    13021 ggttaacgtt gtattctgtt tttgccaacg gtgaggcaaa ccctcgaaca accactttca
    13081 cacgtgctcc tttttgcaat tcttcgagca ataaatctct aaacaacacc aaatctttca
    13141 caccttggtc aacgtattgt gtgaaaaagt tctcaatatc ttcactcgct tcttcggctt
    13201 tatctttatc caaaccaact gcatattctt tttgataacg tggcatcatt gccgtataat
    13261 cgttataagt tgtcatgtag ctcaatttgg taacggtgtc ccacgagcgt ggatctggaa
    13321 tatcgttatg aaaatacaag gttacgggca aacgtttgct caattcttcc aatgtttctt
    13381 taatcgtggg cggattttct ttcattggcg gatacaatgc aaaaatatcg ctgcaacaag
    13441 ttatgttttt gctgtaaagc acaccaatgc ggttgctggt aacataactc gtatcgttgc
    13501 ggttgaaata atacaaatcg ttggccggac tattgatggg ttgccccaca ttttgcggtt
    13561 cctcaaactg accatctttg tattgcgaat aaaaaacatc aaaaccacca aagccgttgt
    13621 gccacgtaga ggcaaaatac aaacgttgct gatttttatc ccaaaacggt gttacttcgt
    13681 tgtcaatggt gttgagcgat ttaatggatt tcacccttcc aaattggttg ccgttggtga
    13741 tagtggtgta aaataaatcc aatccacctt ttgtttcttg gctgttgtcc gagcaaaaaa
    13801 acaatacttc tttgccgtct aattctccaa ttgcaggcat agtggtattg gcattgggcg
    13861 cattgatgat ttctcccaat gtgtccacca ttccccattt tccgttaaca aattttgcca
    13921 ccactaattt acattgataa ctaatgccat ccacatcttt gcacgaacta aaatataggc
    13981 gttttccatc caatgacaaa gctgcattgc ccgtattttc attattcgag gttaattctt
    14041 gccatttgat tgaattatca aacctgcctt cttcaaattt ggatttaaaa agagaggtgg
    14101 tataatgttt ttcatacact tcttcattgg cactgatgga atcggcacga agggatgaaa
    14161 aaaccaattc gttcaacaca aaattgtgtc caaattcaga gtttttggta ttcaccgttt
    14221 cgggtaaacg cacaaaatca cctttttcaa caatatcttc ccttgatttt gcccaaagtg
    14281 tggattctaa ttcttgtttg gatttaaggt acaaataatc ttttttcttg tccttgtatt
    14341 ttttcttagc ctttttaaac gtttctatag cttgatcgta ttttccgttt tgtttttgca
    14401 tcaatcctaa ttgcaacaaa ctggaaggat atatttttcc caattcacgt tcatacactt
    14461 tagcgtagta atattctgcc tttctataat ctttgtaggc acggtgtgtt tctgctactt
    14521 tccacaaaat atcgatggtg ttggaatcaa tttccatggc ttttgaatac aattccaagg
    14581 cataataata atcgcctttt ttgtattttt catcggcaac tttgaggtat ttggatagca
    14641 tattttgtcc ccaagaaatg gagaaaacac aacaaaacaa cgctattaaa tataatctgg
    14701 gcatatacgg tgaacaattt tcttaggttt gaatcggaat aaaatataac gcacagcaac
    14761 ttcaaaacca cctcttactc tactggcttt tgttaaggta gataagttca tgtcgtagct
    14821 caagcccaca aaccattgct ggtaatccat ccctacagtt aaataggccg cgtcaactgc
    14881 acggtaccaa attccgccat acactgctgc atattttgct aaacgattga caatggtgta
    14941 tttaacagac gaacccacaa taaactctcg gtattttcct tgaaaagaga attgaaaact
    15001 cggaatgatg tcccaatcca acccaatcgg atagtaaaac ttagcaaaaa ccgacgtgcg
    15061 aatgtcgcgg tgaatttttt cattaaaaaa cccttgattc ggtcggttta aattaaaaac
    15121 gcccactcct gcctccaagg tttttcgttt gccaattaaa taacggtaaa cagcgcccac
    15181 gcccacacta aaattggttt ttcggtcgga ttgatacaat tcgttggttg gtaaattggc
    15241 atcgtaatat tggccattgt attgatttcc aaattgaaaa gcattccaat tcacttgtcg
    15301 gtggttcatc cccaaattaa ttcccggggt aatggtatgg gtagaatctt tggttaattt
    15361 gagtaaataa gccacatttg cttgtagttc aatagttctc aatcggccat cacctgtaac
    15421 atcgtgaaaa aacattccac ctattcctaa atttcggtaa ttgtacagtt tgccatcagc
    15481 agaaacagaa attgtttggt agggaacaga aaccgctcgc cattgatctc tgaaattacc
    15541 aacaaaacgg atgtcgccat taaaattccc tgtatttcca gggttttgaa aaataggatt
    15601 atcgttgaat tgcgaccaat gaatatcttg cgcacctgca atttgcgtca acaacaaagt
    15661 aacaataaga gcacaaatgt ttttcatttt tggtaatagt caacgatgaa attactaaat
    15721 aaatctaatt ttttcaataa atcgtcaaaa cattccaaaa attacaacga atcaatttgg
    15781 tgcatcattt gaagtccacc acccgatgtg gttggagttt caatgggtgt ttcttcttca
    15841 acttctggaa aaacaatggt tctttccaat tcttctgttg ggataaacgg gcgactagtg
    15901 agtgggaata aatccaacaa ttcaccgctg taataaaact caaaacacaa accaccaaag
    15961 gtataatcgt ggtcgccaac tttaaaattg gagccaccca cttgcggact agggccgggt
    16021 ttactttttt ggtaacccaa attaaataag gatgccaaaa tttctggacg ataggccaac
    16081 gtataacctg ctttttccca atgcgattgg tattggcgca acaacaaagc ggtgtataaa
    16141 taggagtaat aatgcgttcc accttgaatc aaacgttgaa ttgtaggaaa tttatgtgct
    16201 tcaatggaat caattacaga acctggaaaa gtatctttta aattgatgca tttttggtaa
    16261 taatcgcccg gataaaatgg gctgttgggt gtaaacaagg tgttttcaat acgcaaagcc
    16321 gtactttcca aaattccagt aacaccgtat cctctatttt tgggcaagaa tatgtggttg
    16381 tatttataca cttggttttt caacttttca cgggaggagt tgaacatgcg gatttgctcg
    16441 cccaccaagc acatcacaat caaacgcgat tcaacaccgg taattttggc aacagaatca
    16501 atggcttttt tgtcggcttt gacatatttg caaaaatctt tccactcggt gtaattggcc
    16561 caaggataca atgaaagtgt attatcgtct ttttttgctt caatttctct tacttttttc
    16621 tgatacgcct tgtcgttttt aagcaataag ttggttgcat ccaacatgcg caaagccacc
    16681 atcaaatcac cgcttttttc ataggctgcc aaaatggttt tagcattgta gggataatgt
    16741 tgtgccaaca aacccacacg ttgatacatt tgacgttctt cgctctcaaa attcaaactg
    16801 tccaacttaa atccttggtt atatttgttg gccatttgcg caaaataacg gttgttgaca
    16861 tccacttttc cttggtcgtt ggtccactta aatttagccg caacggcaat accaatcatt
    16921 acaaaggcaa aaactgccaa tatgtgaatt ccgatgaaat aagtgatttt aatccacttc
    16981 caaaaacgtt ttttaggacg tttttctaca cttccttgct gttccatttg aaaatagcta
    17041 tttagctaaa agaattgagt tccaacccat tgtattattt gtttttaaaa acaacagtgc
    17101 aaatatgcta cttttttaat tgcgttgtat gttggggtgc actatttttt aatttggaag
    17161 ataattgttg caacatttca tcgcattgta gtgctaaata tctgtgcttc gtttttgcat
    17221 tttcactatc tttgcaccac ttaatcaaaa tttgtatgca aaaagataaa ttcacagtca
    17281 cagctgctct tccttatgca aatggacctt tacacttggg acacgttgcc ggtgtttatt
    17341 taccagccga tatttttgtt cgtttcttgc gaatgaacaa ccacgatgta gcatttattt
    17401 gtggaagtga cgaaaacggt gctgcgatta ccttgcgtgc aaaaaaagaa ggtatcacgc
    17461 ctcaagaaat tgttgataaa taccacacca ttattggcaa ttcatttaaa gattttgaca
    17521 tttcatttga tatttttcac cgtacatcgt tgcctttgca ccacgaaact tcgcaagagt
    17581 tttttaaagt gttgcatgcc aacggatcgt ttacgcaaga aacaagtgag caatattacg
    17641 acgaagaatt taaacaattt ttggccgaca gatacatcac tggagaatgt cctaaatgcc
    17701 attctgccaa tgcttatggc gatcagtgtg aaaaatgcgg ttctgcactt agtccgcttg
    17761 aattgattaa cccaaaatct actttgagtg ggaaaacacc tgtgttgaaa caaacatcgc
    17821 actggtattt gccaatgcaa cgccacgaag gttggttgaa agaatggata caaaaaggaa
    17881 ctttggacgg tgtgcaacaa cacgagccta aaaaatggcg caatcaagtg ttgggacaat
    17941 gcatgtcgtg gatagacgga ggcttgcatt cacgtgcaat gacccgcgat ttggattggg
    18001 gcgtaaaagt tccgcttcca gatagcgaag gaaaagtatt gtatgtatgg ttggatgcgc
    18061 ctattggtta catttcggca accaagcaat gggctatgga taacggtaaa aactgggaag
    18121 aatattggaa aggcgaccgt aaattggtgc attttattgg aaaagacaac attgttttcc
    18181 atgccataat tttccctatt ttgttgaaag ctcacggcga tttaatcttg cccgacaatg
    18241 ttccagccta tgaattttta aatttagaag gtgaaaaatt ttcaacttca cgcaattggg
    18301 ctgtttggtt gcacgaatac attgccagtt atccagagcg cattgatgaa ttaaaatatg
    18361 tgctttctgc cattgcacct gaatccaaag acagtgaatt tacgtggaaa gaataccaag
    18421 ccagagtcaa caacgaattg gccgatattt taggcaactt tgtaaaccgt gcattggtgt
    18481 tgacccaaaa atattatggt ggagctgttc caaaattagg cgaattgacc gatgctgatc
    18541 aacaagtgct tgctgaaatg aaggcaattc ctgcaaaaat tagtcagttg atttacgact
    18601 ataaattgcg tgaagcacaa gccgaagcaa tgaacttggc acgtttagga aataaatatt
    18661 tggcagaaaa cgaaccatgg aaattggtaa aagaaaatcc aactcgtgtt gaaaccatca
    18721 tgaacattgc cttgcaaatt acagcaaatt taagcatcgt tttacaacca tttttaccaa
    18781 aaacagctca aaaattgagt aaattcttga attttgaaac aaaatcatgg gaaaatgctg
    18841 gaaaaatgga tcttttagca gaaggacatg caaccaacca accagaaatt ttatttacaa
    18901 aagtggagga tgcaatggtt gaagctgaat tggaaaaatt gaaaaacgcc aatcctgttg
    18961 ccgcatccaa tttcaatccg caaaaagaca atgcaacttt tgacgatttt acaaaaatgg
    19021 acattcgttt gggaaccatt cttgaggcag aaaaagttga aaaagccgat aaattattga
    19081 aattattggt ggatacaggc attgataaac gcaccattgt ttcgggtatt gcagcgcact
    19141 accaaccaga agatgtggtg ggaaaaaccg ttgctgtatt gattaacttg gaacctcgca
    19201 aaattagagg tgtagaatcg caaggtatga ttttaatggc cgaaaacgaa aaaggagagt
    19261 tgagctttat ggtaccagaa aaaggttttg gtgctgggtt tgaaattcgt taattgaatt
    19321 tattttcagt tgtattccaa tttacaaata acaaggcaat aaaaaatcca aatagggtga
    19381 cgcctaattg agtttctacg gtatcttctg taagaaaaga gctgattagt accacaacaa
    19441 ttgaaatatt caataggttg ttggatttcc aagcttttat caacaaagct cccattagaa
    19501 gaataaaaaa caaagctccc aatatgccat agctgacacc atacgttaaa aacaagttgt
    19561 gactgcgcac tctgttttcg ggtgaaagtg gactatttgt tgcttcataa gctgcgtcaa
    19621 aagctttttg attccctcct gttcccacgc ctaacaacca attgtgttga ataatgtaaa
    19681 gtgaggtttt ccaatgttcc aatcgttgca ataaggaatg cccattagga tcttgattgt
    19741 tgagcaattg gtatttgatt ccatacaaac gcggcatcaa caattcgttg cgttcattgg
    19801 gataagtata tccatcttca atattgcgaa tgtcttgtgc cgataattgt tgaaaccctt
    19861 ttttgtcttt ggtaagcccc atagccgtca tgtaacgtgc acaggtaaca cgcaacatcc
    19921 caccacgctt atcggagcgc atcaaatcca ttttggatac tttttgccac tcatgaaaca
    19981 gctcaataac cgaataatat gcaaatattg tcttgccatt gatttcagaa aatttatcgc
    20041 attcgtggtt atacggaacg ccattttcgg ttactgcggg caatgtggcg caatcaattt
    20101 tttcgtgttg gatgtttgtc acaagaaaca acaaaacacc caatcccaca actacgctac
    20161 cagcaaacaa ataggcaatg ctttttttcc attgcgctaa gaaataaaac aacaaaacaa
    20221 ccactaccaa taccaaggca agaatgctgg aaagaatggc agaataaaac gtataaaatg
    20281 taaaccaaat agctaatgca gtccaacccc aattccattt ttttgcttgc cattgcagcc
    20341 aacaagcgct acaaatgccc aaaacaatca gcaaaccata gcgaatgtga gatgcaaaac
    20401 gcgacattcc tcgtatatct tcataaatgt tgttttttaa gtacaattga aaatacacca
    20461 cattaaaaat ggaggttgcc aacaatgtag ctaaaaataa atagagcaaa taatagtaat
    20521 attttacttg ctgttgcgga tttgttgcca caatcaaagg tagaaaaaca aaaggcaaac
    20581 gcgattttaa atctttcaat ccttgcatgg tatcccacga ccaactaacg ccaacaataa
    20641 acaacacata aaaacctata agcagcatca acgtgcggtt ggaacgaatg atttgccatt
    20701 tctgcacaaa ttgtccctcc aaaacccaat tagcaatagc aaaaacaagc ccaaacgaca
    20761 tcaaaacttt ggtcgtaggc atgcctattg caatcaatgc aaaggaaaaa aattgtatca
    20821 acgagtgaat acgttgtgaa aaaattcctt gttgcatttg aacgagttga tgtactaaaa
    20881 acgaattatt tctttaaaat tgtattgtat tgttgcgtat tgttgagcac ctctattcct
    20941 tttttcaaca cctcatcatg gttgaaagaa ttttgcactc ttccttgctg aaaataatag
    21001 cgagaaacaa tttcgttttc aattaactgg cgaatttgtg cttcaaattt caccaaatca
    21061 cgttcttttg acggcgtaat tttagccatc aatgcctcgt attctgcttt ggcgtcgtcc
    21121 attaaatttt ctttttcaat ggttttttgc attttcatca attgctcttc agaagctgtt
    21181 gaatacgtaa atgtatcttg aattgcatat tttttaaacg cctcataatc ttcatcagac
    21241 aatctaaatt gttctgctgg cgcaatggaa ggatgcgttt tcacataatc ggtaacgtag
    21301 ttaaacaaca cattgttggc ataaatcatt aatgctaaac gcgataattc gggcgcttct
    21361 accttcatat cgggttcaat accacgtcca tctattacgt cacgaccatt tttggtttta
    21421 aacacgtgta acaatgaatc gggtacatcc tccggttttg cgccttcgtt tctatcgtaa
    21481 tattccaatc gttgcacaca tctgccagac ggcgtatagt attttgcaat ggttagtttc
    21541 atttttgctc catagctcaa atccaacgtg cgttgtacta atccttttcc aaacgatgtt
    21601 tcgcccacta ctacagcacg gtctaaatct tgcaaacttc cagaaacaat ttcacttgca
    21661 gaggcagaac caccatccac caacaccact actggaatgt ttaaatccaa gggttcgttc
    21721 acggttttgt attcacggtt ttcttctacc aatcggcctt ttgtttgcac taccaattgc
    21781 ccttttggta caaacatgtt gacaatatga acggcttcca tgagcaatcc gccaccattt
    21841 ccacgcaagt cgaacaccaa ttttttcata cctttttctt tgagtgacaa gaaattcttt
    21901 ttcacttcag aacttgctgt ttgcgtaaaa ctggtcaatt taatgtaacc cacctcgttg
    21961 ttgagcattc cggcataagg aatatcgggc actttgattt catcacgtgt aattttaacc
    22021 gtttctttag ctccgttcgg acgttctacc tcaactgtaa ttgttgttcc ttttggtcct
    22081 ttcaagttgc ttgaaacttc atccgtattt ttcttttgca tggaacgacc gtcaattgca
    22141 agaattttat cgcctgcacg tatgcctgct gtggctgccg ggcttccttc ataaggttca
    22201 gcaatcacca cataatcacc aattttacga atgatagctc caattccacc atattgtcct
    22261 gtcgtcatca agcggtaatc ctcaatgtct gattcgggat aaaaaaccgt gtaggggtct
    22321 aggtcttcca acatagcgct gattcctgcc accgacattt ttccaatttt aggcgattca
    22381 acgtaatatt tatccaaatt ttcatagatt tcatcaatca actccatgtt tttgatgatt
    22441 tcaaaacgct ccgattgcgc ccacgaaaat tgaatgctga tgataaaaac agctattgca
    22501 ctaagagcaa aggattggat ggtgttattt ttcattcttt tctaatgtag ctaccaatgt
    22561 tagtaataat ttatttactt ttttaaataa aaactgatat tctggttgtt tattgtcacg
    22621 ataaaccaaa aataaaacta attgtttgtt ttttaaattt tcgttggcta aaaaatcttc
    22681 caaaattgtt ttgttcaacc gcaacgattc gcgcaacagc cgtttgatgt agtttctatc
    22741 atgtgctcgt ttaaactgac gttttggaac cgatgtcacc acttgaaaag gtgctttaga
    22801 attgggttga tcaattagtt gataggtcaa atgcaaggga taaacattca gccgttgccc
    22861 ttcttgaaac aacgcatcaa ttgttttttt actacacagt ttgtattctt tcccaaacgt
    22921 ttgattcatt agacaaagat agctgaaaat taaagttgtt ggagcaaggt gcaaaaaata
    22981 tgcgttggat ggtgttcgtc tgacaatctg gcgcaaaaaa aatgaggcat gtattttgac
    23041 aaaacttatg caatgaaaca ccaacaacat ccctttgggt ctatttttga atcggtgatg
    23101 tattcactat tgctactcgt cgttttgtgg ttggtgcaat gggtgcaatc tgtttttcat
    23161 tacgattttt acaaattagg catgttgccc cgcacgtgga gtgggatgaa aggcgttttg
    23221 ttcatgcctt tattgcattc tcgtcacgat attcatcaca ttctcaataa ttcgtttgct
    23281 ttgttggtat tgctcaccat gttgatttat tattaccgtg caattgcgtt caaagtattt
    23341 gttttactgt ggttgatgac tgggctacta gtttgggctt atgcgcaaaa caaaggtgta
    23401 tatcacattg gtatcagtgg agttatttat ggtttggtag gttttctttt tacatcaggt
    23461 gttttgcgca aatatttgcc cttacaagcc atctcgttgt tagtaatttt tgcttatggt
    23521 aatttaattt ggggcatttt tcctgtacaa gcatcggttt cttgggaagg tcattttatg
    23581 ggactggtgg caggtttgct gttggcattt gcatttcgtt caaaagccgt tcaagccccc
    23641 aagtatcaat acgaaattga aaaagaattg ggcattgagc cacccgattt agaagctatt
    23701 tggaacgaaa aacaagccca atttcaccaa caatcaactg cagaagaaag tccaacatcg
    23761 atgttgccta taaaaattgt gtatctctat aaaacagaca ctgagcaaga acaagcttct
    23821 gaacaaggaa acaataccaa tcaatctaca acgtggacta aaaatgacgg tgaaacacaa
    23881 cagttgccaa atcagcacta atttctttcc aatagtccac atcaaaagcc aaacaaataa
    23941 agtcggctgg ttctaaatgc acattttttc cgataaaata acttaccatg tctgaaatac
    24001 cattgttatg tcccaccaac aagatgttat tttcactggt gtaattccac aagaattgca
    24061 acaaatctga ttgggatgca tgatacagtt ggtcgtaaaa agcagcacca tgataacagt
    24121 tgtttttttc taatacagtt tgtatgcctg caaaggtttg ctgtgttcgt ttagcagtag
    24181 aaacaacaca ttcaaatggg actgagatgg gatgttgttc taaaaaagcg gctaataatt
    24241 gcacttgttg tttgccttta tcgttgagtt gacgatcaaa atcatttccc gaaggggcgg
    24301 tgctatgagg ttgagcatga cgcaaaatgt ataagaattt cataattggc aacttttggg
    24361 gagttaaatt atcacttttt taaaatactt agcttataaa ttgcaaaaac gtatcagatt
    24421 gactaatttt ggggtagaaa tataagttta acatttaaaa taaaaagtta tgtcatttga
    24481 attgccaaaa ttaccatatg catacgatgc gttggagcca cattttgatg ctagaacaat
    24541 ggaaattcac catacaaagc accaccaagc atataccact aatttaaaca acgcaattgc
    24601 gggaacagat ttagaaggta aaacaatcga agaaatttta aaagtatgtg cagacaagcc
    24661 tgctgtaaga aataacggtg gcggttttta taaccacaac ttgttttgga cagttctttc
    24721 tccaaatgga ggtggacaac caactggtga attagcagca gctatcgacc aatcatttgg
    24781 ttcatttgat gcgttcaaag aagaattttc aaaagcagca gctactcgtt ttgggtcagg
    24841 ttgggcatgg ttaaacgtgg atggtggaaa attaactatt acgtcatctc caaaccaaga
    24901 caatccaatc atcgagggct cttctgccac accaatctta ggattagacg tttgggaaca
    24961 cgcatattac ttgcactacc aaaaccgtcg tccagactac atttcggctt tcttcaacat
    25021 cattaactgg gaagaagtta caaaaagata caacgccgct aaataattaa cttgtagtta
    25081 tttagtttaa taaaattaaa aaatcctgtt ttcattgaag acaggatttt tttgtacaat
    25141 gttgcttagc tttgaaatag acttacagat ctaattaaac aatgaataag gtaaagttat
    25201 ttttttaaac aaagctgttg tcccactagg gcttaccgtt cttattttct acgtcgtgct
    25261 tccttgaaaa ttacgacggt accccaaaaa aaatctcaaa aaaaagagta agaaactaat
    25321 cttactcttc atctgttgtc ccactagggc tcgaacctag actcttctga accaaaatca
    25381 gacgtgttgc cagttac
//
LOCUS       DAOQVQ010000003        20460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_3067_length_20460_cov_3.832786, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000003 DAOQVQ010000000
VERSION     DAOQVQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 20460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20460
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_20460_cov_3.832786"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..614)
                     /locus_tag="PKN22_00280"
     CDS             complement(33..614)
                     /locus_tag="PKN22_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNS41165.1"
                     /translation="MLKWLKKNKTKIRRLNLATHRDLGYFFSGLIIIYSISGIALNHV
                     NDWNSDFILKKQEVSLKRTYEASEITDKEVLKFGQMVGEEKYNVYDFPTRDQVKIYYP
                     NGTLHVYLSQKKAVYEHVAKRHFFYEANVIHKNSLKGWKWASDIFGFLLIVITVTGMF
                     ILKGKNGIKGRGKWLLLGGIVPPIVAVILQALL"
     gene            complement(630..1574)
                     /locus_tag="PKN22_00285"
     CDS             complement(630..1574)
                     /locus_tag="PKN22_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNS41166.1"
                     /translation="MNTTLFSKGTIAVLLIAMTSLFFSCGNNASKSEAKETSDKKIGL
                     LLVNHGSRSATWRNALMDLEKNVKDRVLASGEVKGIKTAFMEYTEPSIATRLKEFDDE
                     GFTDIIVVPIFLTVSSHTFDDIPTIMGQKTDPQSREAFKLENIKIYKPKARTYIAPNL
                     DFTDILKKNVAQRAKAMSKNPATEGVVLIGYGDAQYDKEWTALFNEVGEYVKKEIGIT
                     EHTYGWCGHLVHYDSQKTTDAINQLLAKKETAIVIPVLVAHDEEFQNNIIGGGVKMVK
                     NYKDKVYYKTDAILPDKNIEQWIVDITAEYLKKVEKSN"
     gene            1774..5232
                     /locus_tag="PKN22_00290"
     CDS             1774..5232
                     /locus_tag="PKN22_00290"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS41167.1"
                     /translation="MITISTNQKLFKYFIISVLCCFTSVYSIAQKDTLFWFGAPNFSS
                     GLGENPISLTIVTYDDPAVVTISQPANGSFLPITLSLAANSYQTVDLSSFITSIESPS
                     ADAINNNGLKISSTAAITATYSVDSPYNKETFGLKGNNALGTNFYLPFQKSLNAVSST
                     PSAYSGFEIVATENNTTVLITPKAAIVGHAINATFSIVLNEGQTYSARDIDGVATTSL
                     AGSIVSSDKPVAITIFEDGLSYNASVNTSCFDAIGEQLMNINLLGNQYIVRKGTSSSN
                     DFVYILATQNGTNLTIHTSTTTTATISWGETHILNLSEDVAYIESNKPVYVYHVSAHD
                     CELSSTIVPSVYCAGNYNTSFIRNSTDDFGVVVYTRAGYEGSFTVNGASGILNATDFV
                     NVPGTAGALKVAQKYFTTTEIPVGVYTSIENTGDIFGLATIHGASTSGYSYSFITEYS
                     STPFVNAGADGAVCANVGFSLNGIVGGGSLNGTWSSTGYGTFTYGLNNLVNEYVPNTL
                     DVFVNPVKIILTSDATCPSQKDTIYLTVNQMPIVNASANQTVCANNSVVALNGTVQGG
                     SSTGQWSTLGSGTFTPNDSTLNANYIPSNNDIANGNVKLVLTSTNNGNCQTETDTMEV
                     FITPPPVTSISQDTIIVCANNNVVSLSGTVSGPTTTGVWNSSGDGLFTPSNTSLSTTY
                     YPGLNDINNGFCWIYLRSTSNGNCLPDKDSVYVQFTPSPSVDAGANQIICTNDAQIQL
                     NGSITGGATTGVWSGGTGSFSPSNTALNAVYTPTPAEISSGNIALTLTATNFGNCNTV
                     NDVVQFIFVAPPHANFSAANNCFGDSSNFVNFSLAGYGSITQTQWNFGDGNSSNNLNP
                     THYYNQSGTYSTQLIVTNSNGCKDTVSNSIVVYDKPNADFNYNVDCQNNVRTVNFTDA
                     STPSNQINYWFYDFGGQGIALTKDASQTFNNPGNYSVLHIVKTSNGCSDTVVKPLQIT
                     PLPEAGFAFNYSSGVNVGTTYNFIDTSNYASSYYWEFGNGGTSNEANPTTIYFQNGTF
                     PVIQYVYDNLGCFDSTIVWVTVNNVTTEISTLIPNVISPNGDGLNDVWKLEFIHLLYP
                     QATVQIFNEWGQLLFQSEGYTTPWDGTYHGKDVPDGNYFYVINLNADVEQPIYKGVLL
                     VLRKKK"
     gene            5236..6201
                     /locus_tag="PKN22_00295"
     CDS             5236..6201
                     /locus_tag="PKN22_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNS41168.1"
                     /translation="MKQLLAIFVLSLLLNPTIGMSQQMGIYSNYMMNTFYYNPAVAGS
                     QGVYVLNAGYRKQWVGFQGAPSLFNFNIHGSYKSQGKIGYGLGVYNENTGLMNTTGIH
                     LTYAHHFKLTENLKLALGVQPGFLQYKIRLYDAIIADEGDQVLLGSIYSTNAVDVNMG
                     FNLYSNKFFVMASAQRMLGKFIKFTGYNSQLQFHYNFIAGYNFSFEKKKFDFQPSILV
                     RYTKPAPVQISGMLKFMYNKQFWLATIYRHNDAIGVNAGFTFKERYTISYGYDFCINK
                     LRKYNTGSHEVMISIILNKKKPTLKEEDDKLNNSILEETKMNSKK"
     gene            6212..9892
                     /locus_tag="PKN22_00300"
     CDS             6212..9892
                     /locus_tag="PKN22_00300"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS41169.1"
                     /translation="MKKIKILFTLLIFLLVTPLAKAQIDTVFWFAAPWVSPGHAQNKP
                     VALRFSTFNTPTTVHLFQPAGTYDTTFTIAPNSLKSVFLDHIINTLESKPADTPLNYG
                     LKIETDEPVTVVYEVLTVVNNPETYSLKGSNGIGLEFVTPFQNRWRNGVYAPLPKQMF
                     CIVATENGTTVWITPRANIVGHPAGVTYSITLNKGQTYTAENVTQLTNTPGNNLSGSI
                     IVADKPIAVTVSDDSVWEQFAGGCRDLMGDQIVPKEVIGNNYIINKGSMNASANEGIF
                     VVATENFTQVTITTINGSTTQLLNQGDTWDYSITEALTYVQADKPIYVLQASGFGCEL
                     GEALLPPINCAGSSQVSFTRTNNQGFFLNILCPTSAVNNFLLNGSNTLVQGSMFSVVP
                     GTGGAWSGAQISFSTAEIAVNSANFIENTTDFFALGIINGGQTSGCYYHYMSSFLRRT
                     YTDAGNDTTLCNGATSISLSGSVKGATTTGIWSVVNGTGTFQNATDLSTLYYPTTSDY
                     TQGELSFVLTSTGSCNPVTDTIKVSFIQAPVVTASGNQTFCKNNLPSIVISGGVQFAT
                     TAIWSGGAGGSFGNPNDLTTTYTPSPAELAADSVALFLTSAGSFFSCPSDQDTVILYF
                     TPAPVVNAGSDVYICSNTTEINLNGLISGVTTTGVWSTNGNGAFSPSEQNLVTDYLLD
                     QSDIAAGNFNLVLTSTNNQDCLAEVDTIQVYITPQPSVNITTQDSICSTSTLIQLQGT
                     MTGGFGSQWSSTGFGSISNPSSLNTYYTVSPVDTTTGYVDFLLNTTGVCAGIYDSIRV
                     FFIEAPVVYAGPNQNLCENAAIQLNGQISGPSPLGSWSSLGTGMFIPGNNFLSTIYQP
                     STGDILNGSVTLILEATNTYGCNVDNDTLIVTFKEIPTADFSVNSVCQGVNTVFIDQS
                     SFSSGTITNWQWNFGNGSTSTIQNPQPIYNNGGDYTVQLIVSSSNNCTDTIEKNLQIY
                     YNPQPGFSNSNACENNPIYFTDLSTIPQGSITNWHYNFNGFDNSTNQHPNYSFPIAGN
                     YPVTLTVTSDHGCQASITQNVVVIQSPQASFTALPNPAVVDQTIQFTDNSTGTNLVSW
                     YWNYGDGSANNQQNSQHSYSTGGVYPVTLTVTDGNQCFDTLTQIISVELLPVLPTGFT
                     PNGDGENDVFIIRGGPFKTVDFKVYSNWGELVFSSTDQNEGWDGTYKGTPSPVGVYTW
                     TFVVEMGNGQIIKKSGDVTLLR"
     gene            9898..10884
                     /locus_tag="PKN22_00305"
     CDS             9898..10884
                     /locus_tag="PKN22_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS41170.1"
                     /translation="MYKFILIPFIFLTFAVRAQDAHLSMYDAAPLFLNPALTGVVDGT
                     FRLHAQYRTQWKAVNFKPYTSYLFSFDMPIKKWSFGVQLNNFRAGFGNFNVFQGLVST
                     SYNTAVNKAKTHFLSFGVQAGVAQKSVEYQLLSFNNQYSLTGGGTFDQTIASGEDFKG
                     QSVFIPAVNAGILYYYAKPTARLNPFIGISAFNLLHSKESFYGVENRLPLRYYAHLGT
                     RINITETFYIIPKILGMLQKNYFEHTYAVELGYYFKNSDFYLLGGIIARAKDAMVVTL
                     GAKWNNFTLKMAYDINVSSLSRVSTGRGGFEIGLTYVHKKPEKKYEKICPRL"
     gene            10903..13002
                     /locus_tag="PKN22_00310"
     CDS             10903..13002
                     /locus_tag="PKN22_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS41171.1"
                     /translation="MKNLLIIFLCMLSFVGLSQSRMVWLNTADKCYENKDYYNALLYY
                     QKVLDDTLALKTDILPYEVQLANRTLSKKAKQLDGKAVSLEDYTNHQIAMCYQLTYDY
                     KRAEAQFAKTYQSKGYPNDAYHYGNALKNNQKYSEAIDVLDAFVRSNSPSDSLVELAI
                     ASIQGCNYMLHDSKAKTEAKVKLADTSIFNKGTASFAAAYFGSYNRLMFTSARDGGVI
                     LKPEQQSAYLCDVYWTEKDDNGKWKTPTNFGRPMNSAQHDAASTFNNNNTIFYTRWSD
                     ENRKEQNIYLARMIDFKFFEAYKLDERVNVPGYISIHPQLSIDGKTLYFSSNRPGGQG
                     GMDIWKISLDTLGNIIGNAENLGKMVNSNADEITPFFHDATSTLFFSSNGYNSIGGFD
                     VFKSGYNPEKSVYSIPSNLGAPINSSFDDTYLIYDAKLDKGFLSSDREPCEFGHCFNI
                     YEISNSEIIVNLEGITYEKGTDKILPNSTITIKDVKGVANNYVVTTDENGYYNVKITI
                     GEEIFLKAQKEGYFADANVINTESITESITLRQDFYLNKIPQKEIRIEGIEYDFDSDK
                     LRPVSMEILDKIVEFLELNDNLVVEINSHTDYRGSDKYNLDLSKRRAQSCVNYLIDKG
                     INKNRLVPKGFGESMPTSYLDTERKEILDSDGKEIILTEKFILTLTPEEQERANQLNR
                     RTAFKVVGENFKLESSK"
     gene            13009..13470
                     /locus_tag="PKN22_00315"
     CDS             13009..13470
                     /locus_tag="PKN22_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41172.1"
                     /translation="MKNLLLFLTLLLSITVYSQKKDIKIKDDIVTVDGTAVFKITTVS
                     MGNLTTIYNLQGERLIVFTYQTYNDEAKKSQANPSGAVRFLDVTFLNEAMDKCEIGVD
                     TKKGMVKQVIDNELIDENGKLNDKMIQQFVRIHGTKFSDNKKTSTTIIINN"
     gene            complement(13478..16879)
                     /locus_tag="PKN22_00320"
     CDS             complement(13478..16879)
                     /locus_tag="PKN22_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41173.1"
                     /translation="MSSTAFGQASTLRVKEIASTSDTLQLDTLSIFPQSFQLFCNGEL
                     VAHDDYYLNGAKAQLIIKNRCGGNLSAQYRVFPYNLTQKFQKRDTSFIYESAKGNKDF
                     YVYSAQTQIQDVFGGTSLNKSGSISRGISFGNNQDMGINSSLNLELSGEIGPNLKLIA
                     SLSDDNIPIQPDGNTNKLQEFDKVFIQIYNDRLKLIGGDFWLDKPHGYFMNYKKRAQG
                     LSIGYDFRNEDKIGFTTQVSGAMSRGKFNRQIIQGVEGNQGPYRLTGAENEPYIIILG
                     GTEQVFIDGRKLERGQDFDYIINYNSAEVIFTSKNQITKDSRIVVEFQYSDQNYARFL
                     VQTANRYQFKKLDLWFNAYSEHDAKNQTIQQDLTIEQKKMLAAIGDTLTLARISSVDS
                     VGFIDNQIMYKIVDTLGYDSVLVHSVDPTNAVYRATFMNVGQNKGNYVLKEYIATGKV
                     YQWVAPVGGVPQGDYEAYRVIFTPKKRQMLTAGAKYQITKYLTAETEWSYTNNDLNTF
                     SKYDRRDDDGYAAKARINGKIPLSRDSSQRWLMDTKLDFEALDRNFTPIQQYRAVEFD
                     RDWNTRGKGYVGNQIATTLGANFIHRKYGNFNAEGQQFLVGKDYAGYRAVLNGSWQQK
                     GFKAIWDGSYLESKAVDKNQYLRHKVDISQSLKWLRVGFKDDHERNAFQSPTIALARN
                     SYQFYDYQCYIENGDSMKHKYRVFYRERYDWISDSFNLKNAARARTAGGEVRLTEIKN
                     QTLTIQTSYRQLKILDSLLINQTPENSILGRIDYGLRLWKSAFTWNTFYEVGSGLEQK
                     KEFLYIQVNTGQGIYTWIDYNNDGVKDLNEFEIAQYADQASYVRVFTPSSEYVKTYSN
                     EFNQSLFWRPERIWASKKGILKVLSRFSDQIRIRINRKTNYFDGANAFNPFATNVRDT
                     NIISTSYDFRNTFYFNRTSSVVSADWTYQNNQGKTLLASGFDSRKNEYHEANVRWNIT
                     KKIAIETKGQIGNKTAIADYTSGRNFSIDYFTIRPTFSLQTSTAFRVSLTGRYEEKHN
                     RDEFGGENAYVGEIGTNIKFNEAEKGSLQAEFKVVSITYNGTQNSALGFEMLESLKPG
                     VNYTWSIGYQRLISKNLQLSIQYNGRKSKNNKMIHSAGMEVRAFF"
     gene            complement(16962..18248)
                     /gene="murF"
                     /locus_tag="PKN22_00325"
     CDS             complement(16962..18248)
                     /gene="murF"
                     /locus_tag="PKN22_00325"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNS41174.1"
                     /translation="MENFYNYFLQSSGVCTDTRNISKDCFFVALKGATFNGNTFAQTA
                     IDNGAKYAVVDEEAYANEAQNIFLVDNCLRFLQQLANYHRKQFSIPVIGITGSNGKTS
                     TKELLAAVLQKKYNVHFTKGNLNNHIGVPLTLLQLNSSHEIAVIEMGANHFGDIKELC
                     DIAEPNYGIITNIGKAHLEGFKNFEGVLKTKKELYQAVEANNGTLVYNADDEVLYNNL
                     PSNVPTFAYGANNGKVVGKLERLSPFVELSWSMDTYQSGTIFTNLVGKYNFYNFLAAI
                     SFGVLFDVEPAKINEAIGEYNPTNKRSQVVKTTKNTLILDCYNANPTSMRSALESFAL
                     IDNPSKFIIIGDMLELGEESKKEHQAIVELVKELNLQGIVVGNEFSNSVLPTTLVNYT
                     KTDDVKALLSQQPITDKLILLKGSRGIGLEVLEEYL"
     gene            complement(18293..20317)
                     /locus_tag="PKN22_00330"
     CDS             complement(18293..20317)
                     /locus_tag="PKN22_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNS41175.1"
                     /translation="MKISVFKVLVVLGLGSVLFSSCSKEASSSTGWDYNNPKQGGFQK
                     VPFIDQETGPGLILVEGGTFTMGRAEQDVMYDWNNRPARVTVSLFYMDQTEVTNFHWL
                     EYLYWIGRAYGESYPLVYKNALPDTLSWRSPLGFMEKYVDYYLRHPAYRDYPVVGVSW
                     LQANDFCKWRTDRVNEYILIREGILPWNVDQRNDQTFNTDAYYVGQYEDAGMRGVTDL
                     NPTNKQGKKLGTRIVRMEDGIMLPRYRLPTEAEWEYAALGLIGNLEAGSENITDRRFY
                     PWNGHWVRQDNQEFTGKIRANFVRGNGDYMGIAGNLNDGADVTAPVESYWPNDYGLYH
                     MAGNVSEWVLDTYRPLTSEDFNEFRPFRGNVFKTKLLNNEGLVDIKNQQVEYDVHGMK
                     EYLNEFERIRYQRISADKHNPNDTVIPIGLSKNIESANPVHKGYAPDPFYVSHGKVKD
                     SIELSLLRQINEVLDLAIKYKNDKYNTEASALIQDRLFDGIFADEIRTGPDGEEYAFE
                     VLSMLRTAFSEFITDTPGKLKYRNVTEEENIGRLNYRKDDYVDYLDGDIESSVYYKDD
                     ALKMKINQANRDPNLVMYQNKHEEYDLDGNLIKPGQDNRTSWPTTLISDKSKVYKGGS
                     WADRAYWLVASNRRFLDEDKSMCTLGFRCAMDRVGSPVGMNYGKKKKKKK"
BASE COUNT         6413 a         3928 c         3464 g         6655 t
ORIGIN      
        1 tcttctttct aattttaact taactttatt ttttatagca atgcttgtag tatcacagcc
       61 acaataggtg gaactatacc gcctaaaagc aaccattttc cacgtccttt aatgccgttt
      121 ttacctttta gaataaacat tccggttacg gtaatcacaa tcaatagaaa cccaaaaata
      181 tcggatgccc atttccagcc ttttaaactg tttttgtgga taacgttggc ctcatagaaa
      241 aaatggcgtt tggcaacatg ctcataaacc gcttttttct gactcaaata cacgtgtaaa
      301 gtcccgttgg gatagtaaat cttaacttga tcacgagttg gaaaatcata tacgttgtat
      361 ttctcctcgc ccaccatctg accaaatttc aacacctctt tatccgtaat ttcacttgct
      421 tcgtaggtgc gttttaaact cacctcttgc tttttcaaaa taaaatccga attccagtca
      481 ttgacatgat tcaaggcaat ccctgaaatg gaataaatga taatcaatcc cgaaaaaaag
      541 taccccaaat cacggtgcgt tgcaaggttg aggcgacgaa ttttcgtttt atttttcttt
      601 aaccacttta acattattgc tacttttaat tagttacttt tttcaacttt ctttaagtat
      661 tcagcagtaa tatcaactat ccattgctcg atgtttttat ccggcaagat agcatctgtt
      721 ttgtagtaaa ctttgtcttt gtagtttttc accattttta caccaccacc aatgatgttg
      781 ttttggaatt cttcatcgtg tgccaccaat actggaatca caatagcagt ttctttctta
      841 gccaacaatt ggttaatggc atctgtagtt ttttgagaat catagtgcac caagtgtcca
      901 caccatccgt aggtatgctc tgtaatgcca atttcttttt tcacatattc accaacttca
      961 ttgaataaag ccgtccattc tttgtcgtat tgcgcatcac cataaccaat taacaccaca
     1021 ccttctgtag ctgggttttt actcatcgct tttgctcttt gcgctacgtt tttcttcaag
     1081 atatctgtaa aatctaagtt tggagcaata tacgttcttg ctttaggctt gtaaattttg
     1141 atgttttcca acttaaatgc ttcgcgcgat tgtggatctg ttttttgtcc cataatagtt
     1201 ggaatatcat caaatgtgtg cgaactaact gttaagaaaa tcggcacaac aatgatatcg
     1261 gtaaatcctt catcgtcaaa ttctttcaaa cgagttgcaa tagaaggctc tgtatattcc
     1321 ataaaagcag ttttaatgcc ttttacttcg ccacttgcca atactctgtc ttttacattt
     1381 ttttccaagt ccatcaacgc attgcgccat gttgccgaac gtgaaccgtg gttaaccaat
     1441 aacaaaccaa tttttttatc gctggtttct tttgcttcag acttacttgc gttatttcca
     1501 catgagaaaa ataaagatgt cattgcaatc aacaaaactg caatagtacc tttggagaat
     1561 aatgttgtgt tcattttctt tttttaaata aatttagact aattataaat aaagtggtgc
     1621 aaaaataggt tttttatttc attgaacaat caccaaaaag aggtatttag ataaaatatt
     1681 aaaaatttaa acaatttttt atgattaata cttatatttg caaaaaagac tttagcactt
     1741 cattgggtgc agtactatta caaattaaac agtatgatta ctatttctac gaatcaaaag
     1801 ctatttaaat actttataat tagtgtttta tgttgcttta catccgttta ttcaatagca
     1861 caaaaggata cacttttttg gtttggtgct cctaattttt cttcgggact tggtgaaaat
     1921 ccgataagtt taacaatcgt tacttatgat gatccagctg ttgttacaat tagtcagcct
     1981 gccaatggta gttttttacc aattaccctt tctcttgctg ctaattcata tcaaacagta
     2041 gatttaagca gttttataac ttccattgaa tctcctagtg cagatgctat caataacaat
     2101 ggattaaaaa tcagttcaac agcagctata actgcaacat actcagttga tagcccctac
     2161 aacaaagaaa cttttggact taaaggtaac aatgcacttg gaacaaattt ttatcttcct
     2221 tttcaaaaat cattaaatgc tgttagttcc actccttcag cttactcagg atttgaaatt
     2281 gttgcaacag aaaacaatac cactgtttta attacaccaa aagcagcaat cgttggacat
     2341 gcaataaatg caactttttc aattgtttta aatgagggac aaacttatag tgcgcgtgac
     2401 attgatggag tagcaaccac gagtttggct ggttcaattg tatcttctga caaacctgtt
     2461 gctatcacca tttttgaaga cggcttatcc tacaacgcct cggtcaacac atcatgtttt
     2521 gatgcaattg gtgaacaatt aatgaatatt aacttactag gaaatcaata tattgtaaga
     2581 aaaggtacaa gtagcagtaa cgacttcgtt tatattttgg caacacaaaa cggtacaaac
     2641 ttaaccattc acacatctac aacaacaacc gcaactatta gctggggtga aacccatatt
     2701 ttaaatcttt cagaagatgt tgcatacata gaatccaaca aaccggtata tgtatatcac
     2761 gtaagtgcac acgattgtga attaagttca accattgttc ctagcgtata ttgcgctgga
     2821 aattataaca catcatttat ccgaaattca accgatgact ttggtgttgt tgtttatact
     2881 agagctggtt atgaaggttc ttttacggtc aacggagcta gtggtatttt aaacgctact
     2941 gactttgtaa atgtgccagg aacagcaggc gctttaaaag ttgctcaaaa atattttaca
     3001 actactgaaa ttccagttgg tgtctataca agcattgaaa acacaggcga tatttttggt
     3061 ttggctacca tccatggagc ttccacttct ggttatagct actcttttat aacagaatat
     3121 tctagtacac cctttgtgaa tgctggtgct gatggtgctg tttgtgcaaa tgttggtttt
     3181 tcgctaaatg gaatagtagg aggaggatct ttaaatggaa cttggagcag tacaggatat
     3241 ggaactttca cctatggact aaataattta gtcaacgaat acgttccaaa cacgctggat
     3301 gtatttgtga atcctgtaaa aatcatttta acttccgacg ccacatgtcc gtcacaaaaa
     3361 gacaccatct acttgactgt taaccaaatg cctatcgtta atgcatctgc taatcaaaca
     3421 gtttgtgcaa ataactcggt tgttgcatta aatggaactg tgcaaggtgg ctcatctacg
     3481 ggtcagtggt caacactagg aagcggaact tttacaccca atgattcaac tttaaatgct
     3541 aattacattc cttcaaataa tgacattgca aacggaaacg ttaaattggt tctaacatca
     3601 acaaataatg gtaattgcca aacagaaaca gatactatgg aagtatttat tacacctcct
     3661 cctgttactt ctatttctca agatactatt attgtttgtg ccaacaacaa tgttgtttct
     3721 ttaagtggaa ctgtttctgg accaacaacc acgggtgttt ggaacagtag tggagacgga
     3781 ctatttaccc ctagcaacac ttcattatct accacctatt atcctggatt aaacgatatt
     3841 aataatgggt tttgttggat ttacttgcgt tcaacttcaa acggcaattg cttgcccgat
     3901 aaagacagtg tgtatgtaca atttacacca tctccaagtg tagatgccgg tgccaatcaa
     3961 attatttgta ccaacgatgc tcaaatacaa ttaaatggtt ctatcacagg tggagctaca
     4021 actggtgttt ggagtggtgg aactggaagc ttttctcctt ccaacacagc cttaaatgcc
     4081 gtgtatactc caacacctgc tgaaattagt tcaggtaaca ttgcgctaac actaactgct
     4141 accaattttg gaaattgcaa cactgtaaac gatgttgtac aatttatttt tgttgcacca
     4201 ccgcatgcta attttagtgc tgccaataac tgttttggcg actcatctaa ttttgttaat
     4261 ttttcacttg ctgggtatgg atccatcact caaacgcagt ggaattttgg cgatggaaac
     4321 agttcaaaca acttgaatcc tacccattat tacaatcaga gtggtactta ttcaacacag
     4381 ttaattgtta ccaatagcaa tggatgtaaa gacactgtta gcaactctat tgtagtttac
     4441 gataaaccaa acgctgattt taactacaat gttgattgcc aaaacaatgt tagaacagtc
     4501 aatttcacag atgcctcaac accttccaat caaattaatt attggtttta tgattttgga
     4561 ggtcaaggta ttgctttaac aaaagatgca agtcaaacgt ttaacaaccc tggaaattat
     4621 tcagttttac acattgtaaa aacatcaaat ggatgtagtg atacggttgt aaaacctctt
     4681 caaattacac ctttaccaga ggcagggttt gcttttaatt actcaagcgg tgtaaatgtt
     4741 ggaactacct ataactttat tgatacttca aattatgctt catcatatta ttgggagttt
     4801 ggcaatggag gcacttccaa tgaagctaat cctactacaa tttacttcca aaacggtacc
     4861 tttcctgtaa ttcaatacgt ttacgataat ttaggttgtt ttgatagcac gattgtgtgg
     4921 gtcacagtaa acaatgttac aacagaaatt agcactctta ttccaaacgt tatttcacca
     4981 aacggtgatg gtttaaacga tgtatggaaa ctagaattca tccatttgct ttacccgcag
     5041 gctactgttc aaatttttaa tgaatgggga caacttctgt ttcaatcaga aggatataca
     5101 acaccttggg atggcactta tcatggtaaa gatgtgccgg atggcaatta cttttatgtt
     5161 attaacctaa atgcagatgt agaacaacca atctataaag gcgttttact tgttctacgc
     5221 aaaaagaaat aaattatgaa acaactactc gctatttttg ttttgtcatt gttgcttaat
     5281 ccaactattg gtatgtcgca acaaatgggt atttattcaa attatatgat gaataccttt
     5341 tattacaatc cagcagttgc aggaagtcaa ggcgtttatg ttttaaatgc aggatacaga
     5401 aaacaatggg ttggttttca aggtgcgccg tcacttttca actttaacat tcatggttcc
     5461 tacaaaagtc aaggaaaaat tggctacgga ttgggagtgt acaacgaaaa cacgggtcta
     5521 atgaatacaa caggcatcca tttaacgtat gctcatcatt ttaaactaac cgaaaattta
     5581 aaacttgctc ttggtgttca acctggtttt ttacaatata aaattagact gtacgatgca
     5641 attattgcag atgaaggcga tcaagtgttg cttggaagca tatactctac caacgccgta
     5701 gatgtcaaca tgggatttaa tttatattcc aacaaatttt tcgtaatggc ttctgcacaa
     5761 cgtatgttgg gtaagtttat aaaatttaca ggatataatt cacaactaca atttcattac
     5821 aattttattg ctggttacaa ctttagtttt gaaaagaaaa aatttgattt tcaacctagc
     5881 atattggttc gatataccaa accagctcca gtgcaaattt cgggaatgtt gaaatttatg
     5941 tataacaaac aattttggtt agctactatt tatcgtcaca atgacgctat tggggttaat
     6001 gctggtttta cgtttaaaga aagatatacc atttcttatg gctatgattt ttgtatcaac
     6061 aaattgagaa aatacaatac tggttcacac gaagtgatga ttagcatcat tctcaataaa
     6121 aagaaaccaa ctcttaaaga agaagatgat aaattaaaca atagcattct tgaagagacg
     6181 aaaatgaact ctaaaaaata atgtaagaat tatgaaaaag ataaaaatac tattcacgct
     6241 acttattttt ttattggtta ctccattagc taaagcacag attgacacgg tgttttggtt
     6301 cgctgcccct tgggtttctc caggtcatgc tcaaaataaa ccagtagcac tccgtttttc
     6361 tacttttaac actccaacaa cggttcattt atttcaacct gctggtacgt atgacaccac
     6421 gttcacaatt gcaccaaatt cattaaaatc tgtattttta gatcacatta ttaatacatt
     6481 agaatccaaa cctgcagata cgccgctcaa ctatggttta aaaatagaaa cggatgagcc
     6541 tgtaacagtg gtgtatgaag ttttaacggt agtcaacaac cccgaaactt attcgttaaa
     6601 aggaagtaat ggaattggtt tagagtttgt tacacctttt cagaacagat ggagaaacgg
     6661 tgtttacgct cctcttccaa aacaaatgtt ttgtattgtt gcaactgaaa atggaacaac
     6721 agtttggatt accccacgag caaatatcgt tggtcatcca gctggtgtaa cttattccat
     6781 tacccttaac aaagggcaaa cttataccgc tgaaaatgtt acccaattaa ccaatactcc
     6841 tggaaataat ttaagtggtt ctattatagt tgcagataaa ccaattgctg ttactgtttc
     6901 cgacgattct gtatgggaac aatttgctgg tggttgccgc gatttaatgg gtgaccaaat
     6961 agttcctaaa gaagttattg gaaacaatta catcattaat aaaggaagca tgaatgcttc
     7021 ggctaatgag ggtatatttg tagtagcaac agaaaacttt acacaagtta cgattacaac
     7081 tattaatggc tcaacaactc agctattaaa tcaaggagat acgtgggatt attccatcac
     7141 agaagcgtta acctatgttc aagctgacaa accaatatat gtgttgcaag caagtggctt
     7201 tggttgtgaa ctcggtgaag cactacttcc gccaatcaat tgcgctggtt cctcacaagt
     7261 tagctttacc agaacaaata atcaagggtt tttcttaaac atactttgtc ccacttctgc
     7321 agtaaacaac tttctattaa acggtagcaa cacactcgtt caaggatcaa tgtttagcgt
     7381 tgtcccaggt acaggtggtg cttggagcgg tgctcaaata tcttttagca ctgccgaaat
     7441 agctgtcaac tctgccaatt tcatagaaaa tactactgac ttttttgcct taggaatcat
     7501 caacggtgga caaacctctg gatgttatta ccattacatg tcgtcttttt taagaagaac
     7561 gtatacagat gctggcaacg atacaacttt gtgtaatggc gctacatcta tttctttgag
     7621 tggttctgta aaaggagcaa ccacaacagg aatttggtcg gtagtcaacg gtaccggaac
     7681 attccaaaat gcaaccgact taagtacctt gtattaccca acaactagtg attacactca
     7741 aggtgaattg tcatttgtcc ttacttcaac tggaagctgt aatcctgtta cagatactat
     7801 aaaagtaagc tttattcaag ctccagttgt aactgcgtca ggtaaccaaa cgttttgtaa
     7861 aaacaatttg ccatccattg ttatttctgg tggtgttcaa tttgcaacta cagctatatg
     7921 gtcgggtggt gctggtggtt cttttggaaa tcccaatgat ttaacaacta cctacacgcc
     7981 ttcacctgct gaattggctg cggatagcgt tgcattattt ttaacctctg caggaagttt
     8041 tttctcttgc ccgagtgatc aagatactgt tattttatat ttcactccag cacctgttgt
     8101 taatgcaggt tctgacgtat atatttgctc aaatacaact gaaatcaacc ttaatggctt
     8161 aattagtggt gttacgacta ctggagtatg gtcaaccaat ggcaatggtg cattttctcc
     8221 aagtgaacaa aaccttgtta ccgactattt attggatcaa agtgatattg ctgctggaaa
     8281 ctttaattta gtgctgacat caaccaacaa tcaagattgt cttgcagagg ttgatacaat
     8341 tcaggtttac ataactccgc aacctagtgt caacatcaca acacaagact ctatttgttc
     8401 tacaagcacc ctaattcagt tacaaggcac aatgacagga ggttttggga gtcaatggag
     8461 ttcaacaggt tttggctcca tttcaaaccc aagttcatta aacacatact ataccgtaag
     8521 ccctgttgac acaactaccg gttatgttga ttttctattg aatacaactg gagtatgtgc
     8581 gggtatatac gattccattc gtgtgttttt cattgaagca ccagtagttt atgccggtcc
     8641 taatcaaaat ttatgtgaaa atgcagctat tcagttaaac gggcaaattt cgggtccttc
     8701 accactagga agctggtcat cacttggtac aggaatgttt atccctggaa ataatttctt
     8761 atccaccatc taccaaccat caacgggtga tattttaaac ggtagtgtaa cgctgattct
     8821 tgaagctacc aatacctatg gttgtaatgt ggataacgat accctcattg ttacctttaa
     8881 ggaaatacca actgccgatt tttctgtaaa ttctgtttgc caaggtgtga acactgtctt
     8941 tattgatcaa tcgtcttttt ctagcggtac aattaccaat tggcaatgga attttggcaa
     9001 cggctccact tctactattc aaaacccaca acctatttat aacaatggag gtgattatac
     9061 tgttcaatta atagttagct caagcaataa ttgtacagat acaattgaga aaaacttaca
     9121 aatttattac aatccacaac cagggttttc aaacagcaat gcctgcgaaa acaatccaat
     9181 ttatttcacg gatttgtcaa caataccaca aggaagtatt accaattggc actataattt
     9241 taatggattt gataacagta ccaatcaaca ccccaattat tcattcccga ttgcaggcaa
     9301 ctatcctgtg accttaactg taacctctga tcatggttgt caagcatcca ttacacaaaa
     9361 tgtagtagta attcaaagtc cacaagcaag ttttacagcc ttacccaacc cagcagttgt
     9421 tgaccaaact atacagttta cagataactc tacgggtacg aatctagtaa gctggtattg
     9481 gaactatggg gatggatctg caaacaatca acaaaattca caacacagtt attcaacggg
     9541 aggtgtttat cctgttacgc taactgttac agatggaaat caatgttttg atacgcttac
     9601 acaaattatt tccgtagagc ttttaccagt acttccaaca ggttttacac ctaatggtga
     9661 tggtgaaaac gatgtgttta tcatcagagg aggaccattt aaaacagttg acttcaaagt
     9721 ttatagtaac tggggtgaat tagtgttttc atctaccgac caaaatgaag gatgggatgg
     9781 aacttacaaa ggcactcctt ctccagttgg tgtttacaca tggacctttg tagtagaaat
     9841 gggtaatgga caaataatta aaaaatcagg agacgtaact ctgttaagat aaaagctatg
     9901 tataaattta ttttaattcc gttcatattt ttaaccttcg cggttagggc acaagatgcg
     9961 catttatcaa tgtatgatgc tgctcctttg ttcttaaatc ctgcactaac tggtgttgtt
    10021 gacggcacat ttagattgca cgctcaatac cgtacgcagt ggaaggctgt taatttcaaa
    10081 ccttatactt cttatctttt tagttttgac atgcctataa agaaatggag ttttggagtg
    10141 caattaaaca attttagagc aggatttggt aatttcaatg tatttcaagg attggtctcc
    10201 acttcttata atacagcagt taacaaagca aaaacacatt ttttatcgtt cggtgttcaa
    10261 gccggtgtag ctcaaaaatc agtagaatac caattgcttt ctttcaacaa ccaatattcg
    10321 ttgactggtg gtggaacctt tgatcaaacc attgcttcgg gtgaagattt taaaggacag
    10381 tctgtgttca tccccgcagt taatgcagga attttatatt actatgcaaa accaactgca
    10441 cgtttaaatc cgtttatagg tatttcggca tttaacttat tgcattccaa agagtctttt
    10501 tacggcgttg aaaaccgttt acctttaagg tattatgcgc atttagggac tcgtataaat
    10561 attacagaaa ctttttacat tattcctaaa atattaggaa tgttgcaaaa aaattatttt
    10621 gaacatacgt atgccgttga attaggttat tattttaaaa atagtgactt ttacttatta
    10681 ggaggcatta ttgctcgagc aaaagatgca atggtagtaa ctttaggtgc aaaatggaat
    10741 aatttcactc taaaaatggc ctacgacatc aatgtctctt cactaagccg tgtatctacg
    10801 ggtagaggtg gttttgaaat cggactaaca tacgtacaca aaaaacctga aaagaaatac
    10861 gaaaaaattt gccctaggct ttaatttata gctatctgaa caatgaaaaa cttattgatt
    10921 atattcctgt gcatgctgtc atttgttggt ctatcacaat caagaatggt atggctaaat
    10981 acagctgata aatgttatga aaacaaagat tattacaatg ctttgcttta ctatcaaaaa
    11041 gtgctagacg atacgttggc tctaaaaaca gatatattac cctatgaagt gcaattagcc
    11101 aatagaacgc tctctaaaaa ggctaaacaa ttggatggga aagcagtgtc tttggaagat
    11161 tacaccaacc accaaattgc aatgtgttat caactaacat acgactacaa aagagcagaa
    11221 gcacaatttg ccaaaaccta tcagtcaaaa ggttacccca acgatgctta tcattatgga
    11281 aatgctctaa aaaacaatca aaaatacagc gaggcaatag acgttcttga cgcttttgtt
    11341 cgctccaact ctccttccga ctcacttgta gaattagcca ttgcatccat tcaaggttgt
    11401 aattatatgc tgcacgatag caaggccaaa acagaagcta aagtaaaatt agctgacacc
    11461 tcaatattta ataaaggtac tgcttctttt gccgcagcct attttgggag ttacaataga
    11521 ttaatgttta cctctgcacg tgatggaggc gttatcttaa agccagaaca acaatctgcc
    11581 tatttatgcg atgtatattg gaccgaaaaa gatgataacg gaaaatggaa aacaccaaca
    11641 aactttgggc gaccaatgaa cagtgcacaa cacgatgctg cttcaacttt taataataac
    11701 aacaccatct tttatactcg atggtcggat gaaaacagaa aagagcaaaa catttattta
    11761 gctcgtatga ttgattttaa gttttttgaa gcatacaagt tggatgaacg cgttaatgtt
    11821 cctggctata tctcaattca ccctcaattg tctatcgacg gaaaaacact ttatttttca
    11881 agcaacagac ctggaggaca aggcggtatg gacatttgga aaatttcatt ggatacactt
    11941 ggaaacatta ttggaaatgc agaaaacctt ggcaaaatgg ttaattcaaa tgccgatgaa
    12001 attacacctt tctttcacga tgctacttct accttgtttt ttagctcaaa tggttacaat
    12061 tctattggtg gttttgacgt gtttaaaagt ggctataatc cagaaaaatc tgtttactcc
    12121 atcccaagca atcttggcgc tccaataaac tctagttttg atgacactta tttaatttac
    12181 gatgctaaac ttgataaagg ttttctttca tctgatcgtg aaccctgtga gtttggtcat
    12241 tgttttaata tttatgaaat ttcaaacagt gaaattattg taaacttaga gggtatcacg
    12301 tatgaaaaag gtactgataa aattttaccc aattccacta tcacaattaa agacgttaaa
    12361 ggtgttgcaa ataattatgt tgttacaacc gatgaaaatg gatactacaa tgtaaaaata
    12421 acgataggtg aagaaatctt tttaaaagca caaaaagaag gttattttgc cgatgctaac
    12481 gtaataaaca ccgaatcaat tacagaatct attactttac gtcaagattt ttatttgaac
    12541 aaaattcctc aaaaagaaat tagaattgaa ggtattgaat acgattttga ttccgataaa
    12601 ttacgaccgg tttcaatgga aattttagat aaaattgtag aatttcttga actcaacgat
    12661 aacttggttg ttgaaattaa ttctcacacc gactaccgtg gaagcgataa atacaattta
    12721 gatttatcaa aaagaagagc acaatcttgc gtaaattact taattgataa aggtatcaac
    12781 aaaaatcgtt tagttcctaa aggatttgga gaatctatgc caacttctta tttggatact
    12841 gaacgaaaag aaattttaga cagtgacgga aaagaaatta tcctaacaga gaaattcata
    12901 ttaacgttaa ctccagaaga gcaggaaaga gcaaatcagt taaaccgtag aacagcattt
    12961 aaagttgtcg gtgaaaattt taaattagaa tctagtaaat aacgaactat gaaaaatctt
    13021 cttttatttt taacacttct tttatcgatt acagtatatt cacaaaaaaa agacatcaaa
    13081 atcaaagacg atattgttac cgttgatgga actgctgttt ttaaaattac cactgtatca
    13141 atggggaatt taacgaccat ctacaatttg caaggtgaac gtttaattgt ttttacctac
    13201 caaacatata acgatgaagc aaaaaaatca caagccaatc catcgggagc cgtgcgtttt
    13261 ctcgatgtta ccttcttaaa cgaagcaatg gataaatgcg aaattggtgt tgacaccaaa
    13321 aaaggaatgg taaagcaagt gattgacaac gaattgattg acgaaaacgg aaaactcaac
    13381 gataaaatga ttcaacagtt tgttcgtatt catggaacta agttttcgga taacaaaaaa
    13441 acatcaacaa ccatcatcat taacaactaa tgaatggtta gaaaaatgct ctaacttcca
    13501 ttcctgcaga atgaatcatc ttgttgtttt tggattttct gccgttgtat tgaatggata
    13561 actgcaaatt tttggaaatg agtcgttggt aaccaatcga ccatgtatag ttgacacctg
    13621 gtttgagtga ttccaacatt tcaaagccca atgccgagtt ttgtgtaccg ttgtaggtaa
    13681 ttgatacaac tttaaattcg gcctgtaaac ttcctttttc ggcttcgttg aatttaatat
    13741 tggttccaat ttcgccaaca taagcatttt cgcctccaaa ttcatcacgg ttgtgttttt
    13801 cttcatagcg accagttaat gaaacgcgaa aagctgtgct ggtttgcaaa ctaaacgttg
    13861 ggcgaatggt aaaataatca atactgaaat ttcttcctga ggtataatcg gctatagctg
    13921 ttttgtttcc aatttgccct ttggtttcta tggctatttt tttggtgatg ttccaacgca
    13981 cattggcttc gtggtattcg tttttgcgcg aatcaaagcc actggccaac aaagttttcc
    14041 cttggttgtt ttgataggtc caatcggcac ttacaacaga tgatgtgcgg ttgaaataaa
    14101 acgtatttct aaaatcataa gaagtggaaa taatattggt gtcgcgcaca ttggttgcaa
    14161 acggattaaa cgcattggct ccatcaaaat aattggtttt tcggttgatg cgtatgcgaa
    14221 tttggtcaga aaaacgcgat aaaactttta aaatcccctt ttttgaagcc caaatgcgct
    14281 ctggtcgcca aaataaggat tgattgaact cattggaata ggttttcacg tattcactac
    14341 ttggcgtaaa tacccgcaca taacttgcct gatcggcata ttgcgcaatt tcaaattcat
    14401 tcaaatcctt tacaccgtcg ttgttataat caatccacgt gtaaattcct tgccccgtgt
    14461 tgacttgtat gtacaaaaat tctttcttct gttccaagcc cgaacccact tcataaaagg
    14521 tattccacgt aaaagcagat ttccacaaac gcaaaccata gtcaattctt ccaagtattg
    14581 aattttcggg tgtttgatta attaataagc tatccaaaat cttcaactga cggtaactag
    14641 tttgaatcgt caaggtttga tttttaattt ccgttaatcg cacttctccc ccagccgttc
    14701 gggcacgtgc tgcatttttc aaattaaaac tatccgaaat ccaatcgtag cgttcacggt
    14761 aaaaaacacg gtatttgtgc ttcattgaat cgccattttc tatgtaacac tgatagtcgt
    14821 aaaattgata actgttacgt gccaaagcaa tagtgggcga ttgaaaagca ttgcgttcgt
    14881 gatcgtcttt aaaacccaca cgcaaccatt tgagtgattg agaaatatct accttatgac
    14941 gcaaatattg gtttttatca acggccttgc tttccaaata gctaccatcc caaatggctt
    15001 taaacccttt ttgttgccaa cttccgttca atactgctct gtaaccagca tagtctttac
    15061 caaccaaaaa ttgttgacct tctgcattaa aattaccata ttttcgatgg atgaaattgg
    15121 ctcccaaagt tgttgcaatt tgattgccaa catagccttt tccacgtgtg ttccaatcac
    15181 ggtcaaattc aacagcacgg tattgttgaa tgggtgtgaa atttctatcc aaggcttcaa
    15241 aatccaactt ggtgtccatc aaccaacgtt gtgatgaatc acgtgaaagc gggattttac
    15301 cattgatgcg tgcttttgca gcatagccat cgtcgtctcg cctatcgtat tttgaaaacg
    15361 tgtttaagtc gttgttggtg taggaccatt ctgtttcggc ggtcaaatat tttgtaatct
    15421 gatattttgc tcctgcggtt agcatttgcc gttttttggg tgtaaatatt acccgatatg
    15481 cttcgtaatc tccttgtgga acacctccaa ctggcgccac ccattgatat acttttcctg
    15541 ttgcaatgta ttctttcaac acatagtttc ctttgttttg ccccacattc ataaacgttg
    15601 cacggtaaac tgcattggta gggtcaacag aatgcaccaa caccgaatcg tatcccaagg
    15661 tatccacaat tttatacata atttggttgt caatgaaacc caccgaatca acacttgaaa
    15721 tgcgcgccaa ggtgagtgta tcgccaatgg cagccaacat tttcttttgc tcaatggtta
    15781 aatcttgctg tatggtttga ttttttgcat cgtgttcgga ataggcattg aaccacaaat
    15841 ccaacttttt aaactgataa cgattggctg tttgcactaa aaaacgggca taattttgat
    15901 ccgaatattg aaactccact acaattcgcg agtctttggt aatttgattt ttggaagtaa
    15961 aaataacttc ggcagaattg tagttaatga tgtagtcgaa atcttgtcca cgttccaatt
    16021 ttcttccatc aataaaaact tgctcggtac cacctaaaat gatgatgtac ggttcatttt
    16081 ctgctcccgt aaggcgataa ggaccttgat ttccttccac accttgaata atttgtcggt
    16141 taaattttcc acgcgacatg gcaccagaaa cttgcgtagt aaacccaatt ttatcttcat
    16201 ttctaaaatc atagccgatg gataaacctt gtgctcgttt tttatagttc ataaaatatc
    16261 cgtggggttt atccagccaa aaatcgccac caattagttt taaacggtcg ttgtaaatct
    16321 gaataaaaac cttgtcaaat tcttgcaatt tattggtgtt accatccggt tgaatgggaa
    16381 tattatcatc cgacaaagat gcaattaact ttaaattagg tccaatttct ccgctcaatt
    16441 ctaggttcaa cgaagaatta atccccatgt cttggttgtt tccaaatgag attcctcgcg
    16501 aaatactccc tgatttattc aaactggtgc caccaaaaac atcttgtatt tgcgtttgtg
    16561 ccgagtacac ataaaaatct ttattgcctt ttgcactttc atagatgaat gacgtgtcgc
    16621 gtttttggaa cttttgcgtt aaattgtagg gaaaaacacg gtattgagca cttaaattgc
    16681 caccacaacg gttttttata atcaattgag ctttggcgcc attgaggtaa taatcatcgt
    16741 gtgcaaccaa ttcgccgtta caaaataatt gaaacgattg cggaaaaata ctcaatgtat
    16801 ccaattgcaa ggtatcagaa gttgatgcga tttcttttac acgcaacgtt gatgcttgac
    16861 caaatgcggt agagctcatt gcaataagta ataataaaat cgttacgaaa cttctcatca
    16921 atacgaagat acaacgtaaa ataagaaaaa atcgtgtgtt tttacaaata ttcttccaat
    16981 acttccaaac caatacctcg tgagcctttt aaaagaatca atttatctgt aatcggttgt
    17041 tgcgacaata atgcttttac atcatctgtt ttggtatagt ttaccaatgt ggttggtaaa
    17101 acactgtttg aaaactcatt tcctaccact atcccttgta aattcaattc ttttaccaat
    17161 tctacaattg cttgatgttc ttttttagac tcttcgccca attccaacat atcgccaatg
    17221 atgataaatt tagaaggatt atcaatcaac gcaaaacttt ctaaagccga acgcatgctg
    17281 gttggatttg cgttataaca atctaaaatc aaagtatttt tagtagtttt tacaacttgt
    17341 gaacgtttgt tggttggatt gtattcgcca atagcttcgt taatttttgc tggctccaca
    17401 tcaaacaata ccccgaaaga aatagctgct aaaaaattgt aaaaattgta tttaccaacc
    17461 agattggtaa aaatagtgcc tgattggtaa gtatccatgc tccacgataa ttcaacaaag
    17521 ggcgataatc gttctagttt gcctacaact ttaccattgt tagctccata agcaaatgtt
    17581 ggaacattgg atggaagatt attgtacaaa acttcatcat ctgcattgta aaccaaagta
    17641 ccattattgg cttccacagc ctgatacaat tctttctttg tttttaaaac cccctcaaaa
    17701 tttttaaatc cttccaaatg tgcttttcca atattagtaa tgataccata attgggctct
    17761 gcgatgtcgc acaattcctt gatgtcccca aaatggttag ctcccatttc aattacagca
    17821 atttcatgcg atgaatttaa ctgcaacaag gtcaacggca caccaatatg gttgtttaaa
    17881 tttcctttgg taaaatgaac gttgtatttt ttctgaagaa cagccgctag taattctttg
    17941 gtacttgttt ttccattgct accagtaatt ccaattacag gaattgaaaa ttgcttacga
    18001 tggtaattgg ccaattgctg taaaaagcgc aaacaattgt ccactaaaaa aatattttgt
    18061 gcttcatttg cataggcttc ttcgtcaaca actgcatatt tggcaccgtt atcaattgca
    18121 gtttgcgcaa aagtatttcc gttaaatgtt gctcctttta atgcaacaaa aaaacaatct
    18181 ttcgaaatgt ttcttgtatc cgtgcacacc cccgaagatt gtaaaaaata attataaaaa
    18241 ttctccataa ataaaaaagt ccaccgattt ggtggactta aaattaatta ttttattttt
    18301 tctttttctt ctttttgccg taattcattc cgactggact tccgactcta tccattgcac
    18361 atcggaaacc taatgtacac attgatttat cttcgtctaa gaatcttctg ttacttgcaa
    18421 ccaaccagta tgcacgatca gcccaagaac cacctttata cacttttgat ttatcagaaa
    18481 tcaatgttgt aggccatgaa gttctgttgt cttgtcctgg tttgattaag ttaccatcca
    18541 agtcatactc ttcgtgtttg ttttggtaca ttaccaagtt aggatcacga tttgcttggt
    18601 tgattttcat tttcaaagca tcatctttat agtaaacaga gctttcaata tcaccatcca
    18661 aatagtcaac ataatcgtct ttacggtagt tcaaacgacc aatgttttct tcttctgtta
    18721 catttctgta tttcaatttt ccaggtgtat ctgtaatgaa ttcagagaat gctgtacgca
    18781 acattgataa tacttcaaat gcatattctt caccatccgg acctgttcta atttcgtcag
    18841 caaaaattcc atcaaacaaa cgatcttgaa tcaatgcaga agcttctgtg ttgtatttat
    18901 cgtttttgta tttgatagct aaatcaagta cctcgttaat ttgacgcaac aatgacaatt
    18961 cgatagaatc tttcactttt ccgtgagaaa catagaaagg atctggtgcg taacctttgt
    19021 gaactggatt tgctgattca atgtttttag ataatccaat tggaataaca gtatcatttg
    19081 gattgtgttt gtcagcagaa atacgttggt aacgaatacg ctcaaactca ttcaaatatt
    19141 ctttcattcc gtgtacatcg tattccactt gttggttttt gatatccacc aaaccttcgt
    19201 tgttcaataa ttttgtttta aacacgtttc ctctaaaagg acggaattca ttgaaatctt
    19261 ctgaagttaa aggacggtaa gtatccaaca cccattcaga aacgttacct gccatgtggt
    19321 ataatccata gtcatttggc caataagact caactggagc agtaacatca gcaccatcgt
    19381 tcaagttacc agcgataccc atgtagtcac catttcctct tacaaagtta gcgcgaattt
    19441 tacctgtaaa ttcttgatta tcttggcgta cccagtgacc attccaagga taaaatcttc
    19501 tatctgtaat attttcagaa ccagcttcta agtttccaat taaaccaagt gctgcatatt
    19561 cccactcagc ttctgttggg aggcggtaac gtggaagcat aattccgtct tccatacgta
    19621 cgattcttgt tcctaatttt ttaccttgtt tatttgttgg gttcaaatca gtaactcctc
    19681 tcattcctgc atcttcgtat tgacctacat aataagcatc tgtattaaac gtttggtcgt
    19741 tacgttgatc cacattccat ggtaaaattc cctcacgaat taaaatatat tcgttaacac
    19801 ggtctgttct ccatttacaa aaatcatttg cttgcaacca agaaactcca acaactggat
    19861 aatctctata agctggatga cgtaaataat aatctacata tttctccata aacccaagag
    19921 gtgatctcca actcaaagta tctggtaatg catttttata caccaatgga taagattcac
    19981 cgtaagctct tccaatccaa tataagtatt ctaaccagtg aaagttagtc acctctgttt
    20041 ggtccatata gaacaaagaa acagtaacac gtgctggacg attgttccaa tcatacatca
    20101 catcttgctc tgctctaccc atggtaaaag tacccccttc aacaagaatc aaacctggac
    20161 ctgtttcctg gtcaataaaa ggaacttttt gaaaccctcc ttgtttagga ttattataat
    20221 cccatcctgt tgatgaagaa gcttctttag aacaagatga gaataaaact gatcctaatc
    20281 ctaataccac taatacttta aaaacactta ttttcatttg ttttcttatt atgctaggtt
    20341 gcaaatttat actttaataa ctttattttg tcttttcgtt acaatttctt taaaaattct
    20401 tttagaacga tggacaagaa atggttctga actgtggaat tttcttctta caattcaagt
//
LOCUS       DAOQVQ010000004        17511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_3695_length_17511_cov_4.354319, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000004 DAOQVQ010000000
VERSION     DAOQVQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_17511_cov_4.354319"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2864)
                     /locus_tag="PKN22_00335"
     CDS             complement(93..2864)
                     /locus_tag="PKN22_00335"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNS41176.1"
                     /translation="MSNYQAYLQEIEERKAQGLHPKPIDTAELTNEIIAQIKDANNAY
                     RADSLQYFIYNTLPGTTPAAGVKAQFLKDIILGKDVVAEITPAFAFELLSHMKGGPSI
                     EVLLDLALGADATIAKNAADVLKTQVFLYDADMDRLKVAFEGGNAVAKEILESYANAE
                     FFTKLPDVPEEIKIVTYIAGEGDISTDLLSPGNQAHSRSDRELHGKCMISTKAQEEIM
                     QLRAQHPDKSVMLVAEKGTMGVGSSRMSGVNNVALWTGKPTSKYVPFVNVSPIVGGTN
                     GISPIFLTTVDVTGGIGLDLKNWVKKTDAEGNVVKDANGEPVLEQVYSVATGTVLTIN
                     TKTKKLYNGDQELIDISKALTPQKMEFIKAGGSYAIVFGKKIQTFAAKTLGITAPVVF
                     APSKEITKEGQGLTAVEKIFNKNAVGTTPGKTLYAGSDVRVTVNIVGSQDTTGLMTAQ
                     ELESMAAKVISPIVDGAYQSGCHTASVWDKKSQTNIPKLMKFMNDFGLITARDPKGVY
                     HSMTDVIHKVLNDITVDDWAIIIGGDSHTRMSKGVAFGADSGTVALALATGEASMPIP
                     ESVKVTFKGAMKPYMDFRDVVHATQAQMLKQFNGENVFQGRVIEVHIGTLTSDQAFTF
                     TDWTAEMKAKASICISEDATLIKSLEIAKNRIQIMIDKGMDNEKQVLKGLIAIANRRI
                     AEIQSGEKPALTPDDNAKYYAEVVVDLDQIIEPMIADPDVYNKDVSKRYTHDTIRPLS
                     YYGGTKKVDLGFVGSCMVHKGDMKILAQMLKNVEAHYGKVEFKAPLVVAPPTYNIVDE
                     LKAEGDWEVLQKYSGFEFDDNAPKNEARTKYENMLYLERPGCNLCMGNQEKAEKGDTV
                     MATSTRLFQGRVVEDSDEKKGESLLTSTPVVVLSCILGRTPTMEEYTKAVEGITLTKF
                     APSEKMMAH"
     gene            complement(3020..4258)
                     /locus_tag="PKN22_00340"
     CDS             complement(3020..4258)
                     /locus_tag="PKN22_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41177.1"
                     /translation="MKIITYLLLLLLFKNFHCHAQITGCTDPLATNYDALATQNDGSC
                     TYATTTILPLTSTQLPNEMEETSALLYNENQLVTLNDDTHSSLYQFSFQTPQTFATVQ
                     LPFSNVDWEALAQDENYIYVGDFGNNSTGNRTDLKIYRVAKNSLYTSPEVDTIAFSYA
                     TQTDFSAQATNTTNFDCEAFIATDDKLYLFTKEWGSKQTSIFEIEKTPGTHLAQYKTT
                     WNCQGLITDATYYSNKNTVVLCGYTTLMQPFIVLLYDFTNNDFFSGNKRKINLNLPYH
                     QMKRITTENGLDFFVSNEKLVQGPINNPAKIHQLDLSSYLNPFYNTIEQQKNPIASSL
                     VVYPNPVASMLHFQLANNGLQYTVRFYDASGRLIDQTTVDEQHTAYELKHQLFSKGSY
                     KAVFSSENSSEKYSTSFLVE"
     gene            complement(4262..4567)
                     /locus_tag="PKN22_00345"
     CDS             complement(4262..4567)
                     /locus_tag="PKN22_00345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS41178.1"
                     /translation="MNTSFTHAEEKTLGRIYIFVDGKEGGFIEYLIRADNSINAHETL
                     VHERFRGKGLAGLLFDELIAYSKTKNYKIYPTCSFIVKSFERHQELSDLLAADYKNA"
     gene            complement(4640..7783)
                     /locus_tag="PKN22_00350"
     CDS             complement(4640..7783)
                     /locus_tag="PKN22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNS41179.1"
                     /translation="MSTNHYLKVVNASAGSGKTFSLVKTYIRLLLLDEGENALIKYTE
                     ILAMTFTNKAAIEMKTRIIKALDELAFPDFHQSDYIQTIVNETGLKSEVIQAKAQHYL
                     SSILHRYEDFSVMTIDKFNLRLIRSFSRDLDLPPDFEVVLNEQQVIEEVVDDIIGEIG
                     KNEGLTKLLLYYSEANFEDDKGWNLKQSLVNFGNILTKEKELPYVERLMNMEFSIADY
                     NQHRQELNSIRKIYKTLCNDVFLFHCDHQLHTLKINHKKWFDSSINKCKDAELTDFKN
                     DGKFIFNEKTLVYLEEIATTNSTVAEFLHLINKCRAFFIENGGYSVLLKTYLANYFNM
                     ALLKHFNLALTTKNRQERLIRISEFNTKIANLLQEDTLYIYEKLGTRFRHFMLDEFQD
                     TSRLQWLNLIPLVRESLGGGNQNFIVGDSKQAIYRFKNGVAEQFVELPRIYNPEKDPR
                     IEEISAYFDSMGEKETLEDNWRSAPEIVNFNNDLFLKLRENLDVYQARFYASITQNPK
                     SSKKGYVYVECVNKKSKDEEEDGEKYSNHLPFILNSIQEALDDGFNPGDICILGRKNQ
                     FCTYIATQLSERDYSVVSADSLNIDSELTVKFVLSYFKLRLNPVNQNTIKQFFHRFCE
                     LFEHPLSHYESFFELVGENEQKQFKIASLNLFFLKHPGFEAGFFAPYESIYDLIQKTN
                     QLFGLAELKNPYLHHLADLVFAYESKNGPDLNQFLSDYDNLAKEAKALQIAESDEAIK
                     IMTIHKSKGLEFPVVITVSEFDERLKINKAFAETNNYLIYAQPSVSSPIEELNELYAA
                     ESDSVLMDDLNLTYVAYTRPTTRLYVKSIFNKNRLSTYLKSALSSFENTQITDEKLIF
                     TAGERSQKVDTKKQATDDHFFVPVSSSDNLWFPDIALQDNDELVASDFLSDEQRYGNQ
                     FHAAISSINGKEEIELVVHQMIQEGNAEAQFKQQLLTDLEKLFSSETYNQLFSHAQEI
                     LSEQSFIIAANEQIRPDKIIVKKDKTIVVDYKTGAAQKKYAKQVQQYCSVLQEMGYPN
                     VEGHLFYTKDFILEKVV"
     gene            complement(7807..8817)
                     /gene="fbp"
                     /locus_tag="PKN22_00355"
     CDS             complement(7807..8817)
                     /gene="fbp"
                     /locus_tag="PKN22_00355"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNS41180.1"
                     /translation="MSKIVTLQEFITLKQADFPFATGELSRLLNDIAFASKVVSRDVR
                     KAGLVDHILGAQGNTNVQGEEQQKLDVVADKQFIHVFKYGGEVCGIASEENDDYIAFD
                     DEAGKKGKYVVLFDPLDGSSNIDVNVSIGTIFSIYRRKSEVGTLATKEDFLQAGTEQI
                     AAGYVLYGSSTMLVYTTGRGVNGFTLDPSIGEFCLSHPDMKMPEIGRIYAMNEGNIHI
                     CNPGIKKYVEYCQSNENQTGKPYSGRYIGSLVADFHRSLIKGGIYIYPATTNDPKGKL
                     RLMYECNPLAFIAEQAGGLASTGFERIMEIQPTELHQRVPYFVGSKLMMEKAMSLLEK
                     EN"
     gene            8901..10208
                     /locus_tag="PKN22_00360"
     CDS             8901..10208
                     /locus_tag="PKN22_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41181.1"
                     /translation="MKKLLTLFSLTAFAVGYHYISFAQILTSNGATLYVNGGTVYCNG
                     GVEVNNSSTFTNNGLVVTTNNSTLPKKGNFEILSNAIVSGNGGYNVEQDWVNDATFNG
                     DNGEVVLYGNLEQFITSNNGTATHFNNLSLTGSGIGINSRKTLVNVNASTSANGFLVL
                     NDRELHTATNTFTVQNETPTAISYSNVFNAEGFVSSLTNGSLIRNTNQSSLYVFPVGS
                     SDGVRRFRPVEITPISSTASSYSVRFNNYSADNDGFTLTQNDGSVEVANNLFYHSLKR
                     VGGNVDANVGFYYLPTDDGNWESIGHWYSTQWKDMTEATATTNVNFSVLSKANWSFPT
                     TNDAYVLIKPASPFNIPNVFTPNQDGVNDVYFITSVNLKEYEFFIVNRWGEVVFKSND
                     PSEGWDGTVGGKKCSDGVYFYTLKAKQGETELKKQGHITLTSN"
     gene            10228..11139
                     /locus_tag="PKN22_00365"
     CDS             10228..11139
                     /locus_tag="PKN22_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41182.1"
                     /translation="MKIKKLFIGVLLVGASNFYGFSQNNVGIGTTTPSPDAALDIQST
                     TQGVLVPRLTTAQRTAIASPSNGLMVYDTDVNCFFFFDGAVAAWQNLCNAGPQGPAGP
                     QGPAGPAGSVGATGPQGPQGPAGPAGSVGATGPQGPQGPAGPAGSAGATGPQGPQGID
                     GVVQKYHVQGTSGRLAVTSTTHVLQPGMTQTFTLSASKTVVIWATIGGRTTSSTTGAY
                     ATVDMVLYVNGAFLPTGGWNRFNIVNPTSINSFNTCALNTMITLPAGTHTIELRTART
                     NGTSSVDIGGDASLDTNPGEMTIMVLN"
     gene            11146..11400
                     /locus_tag="PKN22_00370"
     CDS             11146..11400
                     /locus_tag="PKN22_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41183.1"
                     /translation="MKGLSIFSFCFVFSSLAFGQTQSAARISNSTVEHKTPQTEVVSN
                     EQQLMSAKKQAIPPKEVVNTNTTNEVNSNNQIGTARIENK"
     gene            complement(11438..13540)
                     /locus_tag="PKN22_00375"
     CDS             complement(11438..13540)
                     /locus_tag="PKN22_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HNS41184.1"
                     /translation="MKLCIAEKPSVAKDIAEIIGAKTRRDGYYEGNGYQVTWTFGHLC
                     TLKEPHDYNEAWKYWRLEDLPIIPANFGIKVINDSGVQKQFSTIENLVANCQEVINCG
                     DAGQEGELIQRWVLLKAKCKVPIKRLWISSLTEEAIREGFEKLKDSQQYDNLYAAGSA
                     RAIGDWMLGINATRLFTVKFGKRQSTLSIGRVQTPTLALIVQRQKEIDAFKGEDYWEL
                     KTIYRETEFLCQIDRMKSAEKAEKGLEYIKDKDFEITSFEQKEGKEGNPRLYDLTSLQ
                     VEANKKYGYSADETLRHIQNLYEKKIVTYPRVDTTYLSEDLHPKVAGILRQMTYYSRF
                     TERLLAEPIPKLKTVFDDKKVTDHHAIIPTGVNPSGITPDEQRVYDLVARRFIAVFYP
                     ECKVSNTVVMGKVDQLAFKATGKQIIEQGWRVVYADMTSKKEHDEDKIMPAFVEGEKG
                     PHEPFVHQGKTTPPKHYTEATLLRAMETAGKQVEDEEMRELMKDNGIGRPSTRANIIE
                     TLFRRKYIEKKRKNIVATPTGIALIDTIQNELLKSAELTGLWEKKLRLIEKGEYQLDV
                     FKQELYQMVRELTDEVIFNTYAHNFVIPTATTEDKPEKKERKPAEKINLEELDCPKCK
                     AAKIRKGKTAVGCANFAQCGFKIPFELLGKKLTEAQLVQLITKGKTGQIKGFIIPGSN
                     EQKAGKLVLDNTWNVGLE"
     gene            complement(13563..14030)
                     /locus_tag="PKN22_00380"
     CDS             complement(13563..14030)
                     /locus_tag="PKN22_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNS41185.1"
                     /translation="MNHVKWNFDGQETIASEGAVVKWDNDTFFTITYNGNSFFGEVLE
                     ENTENNLLRLKINHREFTVSKKGPLDELISSLGMDVPKIKKLKELQAPMPGRIVSIAV
                     AVGQEVNVGDEILSLEAMKMENVLKAEGTGVVKAIFAKPEQVVEKGQVIIEFE"
     gene            14202..15344
                     /locus_tag="PKN22_00385"
     CDS             14202..15344
                     /locus_tag="PKN22_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS41186.1"
                     /translation="MKKPKVIVSVTNDLYTDQRVDKVCRFLVDQNYDVLLVGRIRKNS
                     LKLTEKPYKTHRMRLLFDKGALFYAAFNLRLFFFLLVRKTDVLVSNDLDTLLANYSAK
                     LFKPKTRLVYDSHEYFTEVPELQGRFAKKVWEKIEAFIFPKLTTIYTVNTSIADLYQK
                     KYGKKLYVVRNVSPLWQATNLLSKKELNLPEDKPLIILQGAGINIDRGGEEAVAAMQY
                     IEKAYLIIVGDGDVVPQLKEYVQKYQLSHKVFFYGKQPYLRMMNYTFHASIGLTLDKD
                     TNINYRYSLPNKLFDYIHTHTAVVCTPLVEIKRIVEHYQVGEVLEQLTPQILAQKINS
                     LLENPVLLKHFQDNCLAAKEQLNWQNETAVLKQIYVEEIDSKRNKN"
     gene            15359..16270
                     /locus_tag="PKN22_00390"
     CDS             15359..16270
                     /locus_tag="PKN22_00390"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS41187.1"
                     /translation="MIEKNKIGLSILIPVYNYPINVLWNSLVAVLQSFNNPYEIIFLD
                     DCSTDVAIYKANKALVKNESNVVFEQADYNVGRATIRNRLAEKAQYSYLLFIDVDSKI
                     NNSNFLTSYWSNRNTAKVIVGGTHYSTCPNPSVSLRFYYGKAREEVLASKRNIKPYDS
                     LALNNVFIERQTFLEHKLDESIKNYGHEDTKFGLELKEKNISILHIDNSVEHIGLEEN
                     KVFLGKTIKGVENFALLVQQGYAHDSKLYKTYFLLKRLYLLKLFNFLYSFTSKIISNN
                     LISSRPSMVCYDVFKLYHFIKKMHLEQ"
     gene            16513..>17511
                     /locus_tag="PKN22_00395"
     CDS             16513..>17511
                     /locus_tag="PKN22_00395"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41188.1"
                     /translation="MKTHYFIAICILCCLGGLQNVFSQIYEIDAYNGQTINLTTIPCG
                     SKFYDSGGSAGNYLANENHTVSFYSGAACKQVVVKFNSIYLENNYDKLFVYDGPTDLS
                     PLIQILTGNSFSGTIVSTSNYLTFKFTSDVSIQYAGWDADIISCPTLSYESGCAVAAP
                     LLANSNGVFSFSTPPGDGAVINSTTGVITNGTQGATYQVHDACWNLNATVTMSNTPPC
                     YNMNGDAQVLTVGGNTCVQLTPEANTKTGCAWSEQKVDFNSNFTLSLDYYFGDNPGGA
                     DGTTFTFQPNPIASCGQNGDQLGAGGIPNALIVEFDTYDNDGAANSDLACDNIAVET"
BASE COUNT         5277 a         3281 c         3135 g         5818 t
ORIGIN      
        1 agctattttt taaaacataa aaaatggccg gaatttcttc cgaccattcg tggtaacaag
       61 taccgccttt tcattatttc ttagcaattt aactagtgtg ccatcatctt ttcagatgga
      121 gcaaacttcg ttaacgtaat gccttcaact gctttggtgt attcttccat ggttggtgtt
      181 cttcctaaaa tacaagaaag taccacaacc ggcgtagaag ttaacaacga ctctcctttt
      241 ttctcgtcag aatcttctac cacgcgacct tggaacaaac gtgtagatgt tgccattact
      301 gtatctcctt tttccgcttt ttcttggtta cccatacaca agttacatcc tggacgctct
      361 aaatacaaca tgttttcgta tttcgtacgc gcttcgtttt taggtgcgtt gtcatcaaat
      421 tcaaatccag aatatttttg caacacttcc caatcacctt cagcttttaa ctcgtcaaca
      481 atgttgtagg ttggcggagc aacaaccaat ggagctttga attctacttt tccatagtgt
      541 gcctccacgt ttttcaacat ttgtgctaag attttcatgt cgcctttgtg aaccatacac
      601 gatccaacaa accctaaatc tacttttttc gttccaccgt aatacgataa cggacgaata
      661 gtatcgtggg tataacgctt agaaacatct ttgttgtaaa catctgggtc ggcaatcatt
      721 ggctcaataa tttgatccaa atcaacaacc acttcagcat aatattttgc attatcgtct
      781 ggagtcaacg ctggtttttc tcccgattga atttcagcaa tacgtctgtt tgcaattgca
      841 atcaagcctt ttaaaacttg tttttcgttg tccatacctt tatcaatcat gatttggata
      901 cggtttttag caatttccaa cgatttaatc aacgttgcat cttctgaaat acaaatagat
      961 gcttttgctt tcatttctgc cgtccaatcg gtaaatgtaa acgcttggtc tgatgtcaaa
     1021 gtaccaatgt gtacttcaat tacacgtcct tggaaaacat tctctccgtt gaattgtttc
     1081 aacatttgtg cttgtgttgc gtgtacaacg tcacggaaat ccatatacgg cttcatagcc
     1141 cctttaaacg ttactttaac agactctgga ataggcatag atgcttcacc tgttgccaac
     1201 gccaatgcca ccgtaccaga atcggcacca aaagcaacgc ctttcgacat acgagtgtgc
     1261 gagtcaccac caatgataat tgcccaatcg tccaccgtga tatcgttcaa tactttgtga
     1321 attacgtcgg tcattgagtg gtaaacacct tttgggtcgc gcgctgtaat caaaccaaaa
     1381 tcgttcatga atttcatcaa ttttggaatg tttgtttgcg attttttatc ccaaaccgat
     1441 gccgtatgac atcccgattg gtaggcacca tcaacaattg gagaaatcac ttttgcagcc
     1501 attgattcca actcttgagc tgtcatcaaa ccagttgtgt cttgcgaacc aacgatgtta
     1561 accgttacac gcacgtcaga acctgcatac aatgtttttc ctggtgttgt accaactgca
     1621 tttttgttga aaatcttttc tacagcggtc aaaccttgtc cctctttagt aatttctttt
     1681 gatggagcaa aaactactgg tgctgtaata cccaatgttt tagcagcaaa tgtttgtatt
     1741 tttttaccaa aaacgatagc gtatgaacct cccgctttga tgaactccat tttttgtggg
     1801 gtcaacgctt ttgaaatgtc aatcaattct tggtcaccgt tgtataattt ttttgttttt
     1861 gtgttaatgg tcaaaacagt acctgttgca acagaatata cttgctccaa caccggttca
     1921 ccgttagcat ctttcacaac attaccttct gcatcggttt tctttaccca gtttttcaag
     1981 tccaagccaa taccaccagt tacgtcaacc gtagttaaga aaattggaga aattccgttt
     2041 gttcccccaa ctattggcga aacgttaaca aatggaacat atttgctggt tggttttcct
     2101 gtccacaatg ccacgttgtt tacacccgac atacgtgaag aaccaactcc cattgtacct
     2161 ttttctgcta ctaacatcac gcttttgtca ggatgttgag cgcgcaattg cataatttct
     2221 tcctgtgctt tagtagaaat catacatttt ccatgcaatt cacggtcaga gcgagagtgt
     2281 gcttggtttc ctggtgataa caagtcggta gaaatatctc cttcacctgc aatgtaggta
     2341 acgattttaa tttcttcagg aacgtctggc aattttgtga aaaactctgc attggcatag
     2401 ctttccaaaa tttctttagc aacggcattt ccaccttcaa acgcaacttt taaacgatcc
     2461 atgtcggcat cgtacaagaa aacttgtgtt tttaacacat cagctgcatt ttttgcaata
     2521 gttgcatctg cgccaagtgc caaatccaat aaaacttcaa ttgaaggccc tcctttcatg
     2581 tgcgacaata gttcgaaagc aaacgcaggc gtaatttcag ccaccacgtc tttgcccaaa
     2641 ataatgtctt tcaaaaattg tgctttaaca cctgctgctg gcgtagttcc aggcaaggtg
     2701 ttgtaaataa aatactgcaa tgaatctgca cggtatgcgt tgttggcatc tttgatttgc
     2761 gcaatgattt cattggttaa ttcggcagtg tcaattggct ttggatgaag cccttgtgcc
     2821 tttctttctt caatttcttg aaggtatgct tgataattgc tcataaaagg aaaaaatttg
     2881 ttttctctat taacggatat tttaactaac acttttttcg aatgatttaa actataaaaa
     2941 attcgatgtg cgaatttaag tataaatttt tgattttact atcgaaatac cgtgaaaaag
     3001 gtagtggatt ttaaggttcc tattccacta aaaaggaagt agaatacttc tcacttgaat
     3061 tttctgatga aaaaacagcc ttgtaacttc ctttggaaaa caattgatgc tttagctcat
     3121 aagctgtatg ttgttcatct acagttgttt ggtcaattaa tcgacctgaa gcatcataaa
     3181 aacgaacggt gtattgcaat ccgttatttg ccagttgaaa atgcaacatg cttgcaacag
     3241 gattggggta aactactagt gaagatgcaa tcggattttt ttgttgttca attgtgttat
     3301 agaaaggatt caaataggaa cttaaatcca actggtgtat tttggcaggg ttattaatag
     3361 gaccttgcac caatttttcg ttggacacaa agaaatccaa cccattttct gttgttattc
     3421 ttttcatttg atggtaggga aggttgaggt tgatttttcg tttgttacca ctaaaaaaat
     3481 cattatttgt aaaatcgtat agcaaaacaa taaagggctg catgagggtt gtgtagccac
     3541 ataataccac ggtgtttttg ttggaataat acgtggcatc tgtaattaac ccttgacaat
     3601 tccatgtggt tttgtactgc gctaggtgcg tgccaggtgt tttttctatc tcaaaaattg
     3661 aggtttgttt acttccccat tctttggtaa acaaataaag tttatcatca gtggcaataa
     3721 atgcttcgca atcaaaattg gtggtgttgg tggcttgcgc agaaaaatcg gtttgtgtgg
     3781 cgtaggaaaa ggcaatggtg tcaacctcag gtgaggtata aagtgaattt tttgcaactc
     3841 tgtatatttt taaatcggtt ctgttgcccg ttgaattgtt gccaaaatca ccaacataaa
     3901 tgtagttttc atcttgcgcc aaagcctccc aatccacgtt gctaaaagga agttgaacgg
     3961 ttgcaaacgt ttgtggcgtt tgaaaactaa attgatagag tgatgaatgg gtgtcgtcat
     4021 tgagtgttac caactgattt tcattataca acaaagccga agtttcttcc atttcattgg
     4081 gtagttgcgt acttgtaaga ggtaaaatag ttgttgtggc gtaggtgcac gaaccatcgt
     4141 tttgtgttgc caatgcatca taattagtgg caagaggatc ggtgcaacct gttatttgtg
     4201 cgtggcaatg aaagttttta aatagtaata gcagtagtaa gtaagtaata attttcatgt
     4261 tctaagcatt tttataatca gctgctaata aatcgcttaa ttcttggtgg cgttcaaaag
     4321 atttaacaat aaaagaacat gtaggataaa ttttgtaatt ttttgttttg gaataggcaa
     4381 tcaactcatc aaaaagtaag cctgctaatc cttttcctct aaaccgttcg tgcaccaatg
     4441 tttcgtgtgc attgatgcta ttgtcggctc tgatgaggta ttcaataaaa ccaccttctt
     4501 ttccatctac aaaaatgtaa attctaccca aggttttttc ttctgcgtgt gtaaatgaag
     4561 tattcatatt gcgttgtttt ttatcaaatt taacgaaaat taaagcaaat caatgttcaa
     4621 acaaggaaaa atatgctgtt taaactactt tttctaaaat aaaatctttg gtgtaaaata
     4681 aatgcccttc aacatttgga tatcccattt cttgaagcac cgaacagtat tgttgcactt
     4741 gtttagcata ttttttttgt gcagcccctg ttttgtaatc aacaacaatg gttttgtctt
     4801 tttttacaat gattttatcg ggacgaattt gctcattagc agcaataata aagctttgct
     4861 cactcaaaat ttcttgtgcg tgtgaaaaca actggttgta ggtttcactg gagaatagtt
     4921 tttctaaatc ggtcaacaat tgctgtttaa attgagcctc ggcattaccc tcttgtatca
     4981 tttggtgcac caccaattct atttcttctt ttccattgat gctagaaatg gccgcatgaa
     5041 attgattgcc ataacgctgt tcgtcggata aaaaatcgga agccaccaat tcatcgttat
     5101 cttgcaaagc aatgtcggga aaccacaaat tatcactcga tgaaacagga acaaaaaaat
     5161 gatcgtctgt cgcttgtttt ttggtgtcta ctttttgact acgttcacct gctgtaaaaa
     5221 tgagtttttc atcagttatt tgcgtgtttt caaaagatga taaagccgat tttaagtaag
     5281 tgctcaacct gtttttgttg aaaatggatt ttacatacaa acgtgtagtt ggacgtgtgt
     5341 aggccacata agtcaaattc aaatcgtcca tcaaaacact gtcactttct gctgcataca
     5401 actcgtttaa ttcttcaatg ggcgaactaa cacttggttg cgcataaatc aaataattgt
     5461 tggtttctgc aaacgctttg ttgattttta acctctcgtc aaattccgaa acggtaatca
     5521 ccaccggaaa ttcaagtccc ttggatttat gaatggtcat gatttttatc gcctcatcac
     5581 tttctgctat ttgcaatgcc ttagcttctt ttgccaagtt gtcgtaatca cttaaaaact
     5641 gattcaaatc tggaccattt ttactttcat aagcaaacac caaatccgct aaatggtgca
     5701 ggtaaggatt ttttaattct gccaacccaa acaattgatt ggtcttctgg atgaggtcgt
     5761 aaatcgattc gtaaggagca aaaaaaccag cctcaaaacc agggtgtttt agaaaaaata
     5821 aatttaaact tgcgatttta aactgttttt gctcgttttc accaactaat tcaaaaaagg
     5881 attcgtagtg cgaaagtggg tgttcaaaca actcacaaaa acggtgaaaa aactgtttga
     5941 tggtgttttg gttgacagga ttgaggcgta atttaaagta actcaacaca aatttaacgg
     6001 tcaactcact atcaatgttc aacgaatcgg cagaaaccac cgaataatca cgttctgaca
     6061 attgtgtagc aatgtaggtg caaaactgat tttttctacc caaaatacaa atgtcgcctg
     6121 ggttaaaacc atcatccaat gcttcttgaa tggaatttaa gataaacggc aaatgattac
     6181 tgtatttttc accatcttct tcttcgtctt tgcttttttt attaacacat tcaacgtaaa
     6241 cgtagccttt tttgcttgat tttggattct gtgtaatgga agcgtaaaag cgtgcttgat
     6301 acacatccaa attctcgcgc agttttaaaa ataaatcatt gttaaaattg actatttctg
     6361 gagccgaacg ccaattgtct tccaaggttt ctttttcacc cattgaatca aaataggctg
     6421 atatttcttc aattcgagga tctttttctg gattgtaaat acggggcaac tcaacaaatt
     6481 gctcggctac accattttta aaacggtaaa tggcttgttt agaatcgcca acaataaagt
     6541 tttgattacc gccaccaagc gattcacgta ccaatggaat taaatttaac cactgcaaac
     6601 gcgaggtgtc ttgaaattca tccaacataa aatgacgaaa acgggtcccc aatttttcgt
     6661 aaatgtacaa ggtgtcttct tgcaataaat tggcaatttt agtgttgaat tcggaaatac
     6721 gtatcaaacg ctcttgtctg tttttggtgg taagtgctaa attaaagtgt tttagcaacg
     6781 ccatgttaaa gtaattggcg aggtatgttt taagcaacac cgagtaacct ccattttcta
     6841 taaaaaacgc acggcattta ttgatgaggt gaagaaattc tgcaacggtt gaattggtgg
     6901 tggctatttc ttctaaataa acaagtgttt tttcgttgaa aatgaatttt ccatcgtttt
     6961 taaaatcggt caattctgca tctttgcatt tgttgatgga cgaatcgaac cattttttgt
     7021 gattaatttt tagtgtatgc aactgatggt cgcagtgaaa caaaaacacg tcattacaca
     7081 atgttttgta tatttttcta atcgaattta gttcttggcg atgttggttg tagtctgcaa
     7141 tagaaaattc catgttcatc aaccgttcca cgtaaggcaa ttctttttct ttggtcagaa
     7201 tgttgccaaa atttaccaat gattgcttta gattccaccc tttatcgtct tcaaaatttg
     7261 cttcagaata atacagcaac agttttgtaa gtccttcatt tttaccaatt tcgccaatga
     7321 tatcgtccac tacttcctca atcacttgtt gttcgttgag caccacttca aaatcgggtg
     7381 gcagatctaa atcgcgacta aacgaacgaa tcaaccgaag gttaaactta tcaatggtca
     7441 tcacactaaa atcctcgtaa cggtgcaaaa tactgctcaa ataatgttgt gcttttgctt
     7501 gtattacttc ggattttaaa ccggtttcat taacaatggt ttggatataa tcggattgat
     7561 gaaaatcggg aaatgccaat tcgtccaacg cctttataat tcgcgttttc atttcaatgg
     7621 ctgccttgtt ggtaaaggtc atagccaata tttcggtgta ttttatcaac gcattttcac
     7681 cttcgtctaa aagcaataaa cggatgtacg tttttaccaa cgaaaacgtt ttcccacttc
     7741 ccgcagaagc gtttacgact ttcagataat ggttagttga cacaatacaa gggtgttatg
     7801 caataattaa ttttcttttt ccaacaagct cattgctttt tccatcatca atttggaacc
     7861 aacaaaataa ggtacacgtt ggtgcaattc ggttggctga atttccataa tgcgctcaaa
     7921 accagttgat gccaatccac ctgcttgttc agcaataaac gccaaaggat tgcattcata
     7981 cattaaacgc aattttcctt ttggatcatt ggttgttgct ggataaatat aaattccacc
     8041 cttgattaag ctacggtgaa aatcggcaac caacgaacct atgtagcgtc cagaataggg
     8101 tttacctgtt tgattttcat tgctttgaca atattcaaca tattttttaa tgccaggatt
     8161 acaaatgtga atatttcctt cgttcatggc gtaaatgcga ccaatttcgg gcattttcat
     8221 atcaggatgc gacaaacaaa attcaccaat tgatgggtct aaagtaaagc cattcacacc
     8281 tcttcctgtg gtgtacacca acattgttga cgaaccatag agcacatatc ctgctgcaat
     8341 ttgttccgtt ccagcttgca aaaagtcttc ttttgttgcc aaggttccca cttccgattt
     8401 tcttctataa attgaaaaaa tagtaccaat agaaacattt acatcgatgt tggaagagcc
     8461 atccaagggg tcgaacaaaa caacgtattt tcctttttta cctgcttcat cgtcaaaagc
     8521 tatgtaatca tcgttttctt cagaagctat accgcaaact tcgccaccgt atttaaaaac
     8581 gtggataaat tgtttgtcag caactacgtc taatttttgt tgttcctcac cttgtacatt
     8641 ggtattgccc tgcgcgccca aaatgtgatc aaccaaacct gcttttcgca catctctact
     8701 tacaacttta gaagcaaaag caatatcgtt taacaaacgc gataattcac cagtggcaaa
     8761 aggaaaatca gcttgtttta gcgtaataaa ttcttgaagg gtaacaattt ttgacatttt
     8821 taaatctttt ttacaaatat agttttttgc aactattttt taaatatatt cgtcatactt
     8881 tataacaaac atactttgct atgaaaaaac tacttacttt attttctctt acggcttttg
     8941 ctgtaggtta tcattacata agttttgcac aaatattaac ttcaaatgga gccactttat
     9001 acgtaaatgg aggcactgtt tattgcaatg gtggggttga agtaaataat tcatcaacgt
     9061 ttacaaacaa tggattggtg gttacaacaa acaactcaac attgcctaag aaaggaaatt
     9121 ttgaaatttt atccaatgcg attgtgtcag gaaatggcgg atacaacgta gaacaagatt
     9181 gggtaaacga tgcaactttt aatggcgaca atggtgaagt tgttttatat ggaaatttag
     9241 aacaattcat aacgtctaac aatggaacgg caacacattt caataattta tccttaactg
     9301 gaagtgggat tggaataaat agtagaaaaa cattggtaaa cgttaatgca tcaacaagcg
     9361 ctaatggttt tttagtttta aacgaccgag aattgcatac agccaccaat acttttacag
     9421 tgcaaaacga aacaccaaca gcaatttcct attcaaatgt atttaatgcc gaaggatttg
     9481 ttagtagcct tacaaatggt tctttgatta gaaatacaaa tcaatcttcg ttgtatgtat
     9541 ttcctgttgg ttcaagtgat ggtgttcgac gttttcgtcc agttgagatt acccctatta
     9601 gttcaacagc aagttcatat tctgttcgtt tcaataatta cagtgctgac aacgatggtt
     9661 ttactttaac acaaaatgat ggatcggtag aagttgcaaa taatttgttt taccattcct
     9721 taaaacgtgt tggtggaaat gtagatgcta atgtaggatt ttattattta ccaacggatg
     9781 acggaaattg ggagagcatt ggtcattggt acagcacgca atggaaagat atgacagaag
     9841 caacagctac tacaaatgtt aacttttctg tattatcaaa agctaattgg agttttccaa
     9901 caacaaacga tgcttatgtg ttgattaaac cagctagtcc ttttaatatt ccaaatgtat
     9961 ttacaccgaa tcaagatggt gtcaacgatg tgtattttat cacatctgtg aaccttaaag
    10021 agtatgagtt tttcatcgtt aaccgctggg gtgaggttgt ttttaaatca aacgatccta
    10081 gtgaaggatg ggatggaaca gtaggtggga aaaaatgttc tgatggcgtt tatttttaca
    10141 cattaaaagc aaaacaaggt gagacagaat taaaaaaaca aggtcacatc acattaacta
    10201 gtaattaact ttttatttat atatactatg aagattaaaa aactattcat tggcgttcta
    10261 ttggttggag cttctaactt ttatggattt tctcaaaaca atgtaggaat cgggacaaca
    10321 acaccaagcc ctgatgcagc attagacata caatctacta cacaaggagt tttggttcct
    10381 cgacttacaa ctgcacaacg cactgcaatt gcttctcctt caaatggttt aatggtttat
    10441 gatacagatg taaattgttt tttctttttt gatggagctg tagctgcttg gcaaaatctt
    10501 tgtaatgcgg gtccacaagg tccagcaggt ccacaaggcc ccgcgggtcc agcaggtagt
    10561 gtcggtgcaa caggcccaca aggtccacaa ggtccagccg gcccagcggg tagtgtcggc
    10621 gcaacaggac cacaaggccc tcaaggtccg gcaggtccag ccggtagtgc tggtgcgaca
    10681 ggtccacaag gtccacaagg aattgatggt gttgttcaaa aataccatgt tcaaggtact
    10741 tctgggcgtt tagctgttac ctctactacc cacgtacttc aaccgggtat gacacaaaca
    10801 tttactcttt ctgcatctaa aacagtggta atttgggcaa caattggtgg tcgcacaacc
    10861 tcctctacaa caggggctta tgcaacagtt gatatggtgc tttatgtaaa tggtgcgttt
    10921 ttaccaactg gtggatggaa ccgttttaat attgtgaatc caacatctat taactcgttt
    10981 aatacttgtg cattaaacac aatgattaca ttgccagcag gtactcacac aattgagtta
    11041 agaacagcta gaacaaacgg aacatcaagt gttgatattg gtggtgatgc ctcattagat
    11101 accaatccgg gtgaaatgac gattatggtt ttaaattaat tgcttatgaa aggattatca
    11161 atttttagtt tttgttttgt tttttcatca ttggcatttg gacaaacaca atcagcagcc
    11221 agaattagca attcaacagt agaacataaa actcctcaaa cggaagtggt aagcaatgag
    11281 caacaattaa tgtctgctaa aaaacaggca ataccaccca aagaggttgt caataccaat
    11341 acaaccaacg aagtgaattc taataatcaa attggaacgg cacgtattga aaataaataa
    11401 atgaattttt aagtcctgtt tactttgtag gcaggactta ttccaacccc acattccaag
    11461 tgttgtccaa caccaatttg cctgcttttt gctcgttgga acctggaata ataaaccctt
    11521 taatttgacc tgtttttcct ttggttataa gttgaaccaa ctgcgcttct gttagctttt
    11581 tacccaataa ttcaaacgga attttaaagc cacattgtgc aaaattggca catccaactg
    11641 ctgttttacc cttcctaatt ttagcagcct tgcattttgg acaatccagc tcttctaagt
    11701 tgattttttc agctggtttg cgctcttttt tttcaggttt gtcttctgtg gttgctgttg
    11761 gaatcacaaa attatgtgca taggtgttga aaatcacttc atcggttaat tcacgcacca
    11821 tttgatacaa ttcttgttta aaaacatcaa gttggtattc gcctttttca atcaagcgca
    11881 attttttctc ccataaaccg gtcaactcgg cactttttaa taattcattt tgaatagtgt
    11941 caatcaacgc aatacctgtg ggagttgcaa caatgttttt acgttttttt tcgatgtatt
    12001 ttcttctaaa aagtgtttca ataatgttgg cacgtgtgga ggggcgacca attccgttgt
    12061 ctttcatcaa ttcacgcatt tcctcatctt ccacttgctt gcctgctgtt tccatagcac
    12121 gcaataacgt tgcttcggtg taatgcttgg gtggagtggt ttttccttgg tgtacaaatg
    12181 gttcgtgtgg ccctttttct ccttcaacaa atgccggcat gattttatct tcgtcatgtt
    12241 cttttttcga tgtcatatcg gcataaacca cgcgccaacc ttgctctatg atttgttttc
    12301 cagttgcttt gaatgctaat tgatccactt ttcccattac aaccgtgttg gaaaccttac
    12361 attctggata aaaaactgca ataaatcgac gtgcaactaa atcatacaca cgctgctcgt
    12421 cgggtgttat gccacttggg ttaacacctg ttggaataat tgcatggtga tcggtaactt
    12481 ttttatcgtc gaaaacggtt ttaagttttg gaatcggttc ggcaagcaaa cgttccgtaa
    12541 aacgcgaata ataagtcatt tgtcgcaaga tacccgccac ttttgggtgt aaatcttcag
    12601 atagataagt ggtgtcaaca cgtggatagg taactatctt tttttcatac aaattttgaa
    12661 tgtgacgcag cgtttcatca gcagaataac catatttttt attggcttca acttgcaacg
    12721 acgttaaatc atacaaacga ggattccctt ccttcccctc tttttgttca aatgaagtaa
    12781 tttcaaaatc tttatccttg atgtattcca accctttttc agccttttca gccgatttca
    12841 tacggtcaat ttggcataaa aactcggttt cacggtaaat ggtttttagt tcccaatagt
    12901 cttcaccttt aaaagcatca atttcttttt gtcgctgaac aattaatgcc aatgtaggtg
    12961 tttgaacgcg accaatggaa agagtagatt gacgtttgcc aaactttacg gtaaacaaac
    13021 gtgttgcatt gatgcctaac atccaatcgc ctattgctct ggcacttcct gctgcataaa
    13081 ggttgtcgta ttgttgagaa tctttgagtt tttcgaatcc ttcacgaatg gcttcttctg
    13141 ttaacgaaga aatccataaa cgtttaattg gaaccttgca ttttgctttg agcaataccc
    13201 aacgttgaat taattctccc tcttgcccgg catccccaca gttgataacc tcttggcagt
    13261 tggcaactaa gttttcaata gtagaaaatt gcttttgaac ccctgagtcg ttgatgactt
    13321 taatgccaaa atttgctgga atgataggta aatcttccaa acgccaatat ttccacgcct
    13381 cattgtaatc gtggggctct ttgagcgtac ataaatgacc aaaggtccag gtaacttgat
    13441 aaccattccc ttcgtaatag ccgtctcttc ttgtcttagc gccaattatt tcagcgatgt
    13501 ctttagcaac agaaggtttt tcagcaatac aaagtttcat agttaattag gagatttagt
    13561 aattattcaa actcaataat tacttgacct ttttctacaa cttgttctgg ttttgcaaaa
    13621 attgctttaa caacacccgt tccttcagct ttcaaaacgt tttccatttt catggcttct
    13681 aacgataaaa tttcatcgcc aacgttaacc tcttgaccaa cggcaacggc aatgctaaca
    13741 atacgtccag gcattggagc ttgcaattct tttaattttt tgattttcgg aacatccatt
    13801 cctaacgatg aaatcaattc gtctaatggt ccttttttgc tgacagtaaa ctcacgatgg
    13861 ttgattttta aacgcaacaa gttgttttcg gtattttctt ccaacacttc tccaaaaaac
    13921 gaatttccat tgtaagtgat tgtaaaaaac gtatcgttat cccacttaac aactgcgcct
    13981 tcgcttgcaa ttgtttcttg accatcaaag ttccatttaa cgtgattcat ctgtttttct
    14041 aatttttccc aaagatatta tttttttaag tttttgagag cgtttttcta cggtggttga
    14101 taaatattca ccagtacaac cttttttgat agatttcaaa ggatggcgtt aaaattcgat
    14161 aatctttata cttttatgca atcaaaagcg tttcatacaa catgaaaaaa ccaaaagtca
    14221 tcgtttctgt caccaatgat ttatacaccg accaacgggt ggataaggtg tgtcgatttt
    14281 tggtagatca aaattacgat gtgcttttag tgggtagaat tcgcaaaaat agcttgaaat
    14341 taacggaaaa accttataaa acacaccgca tgcggttgtt gtttgacaaa ggtgctttgt
    14401 tttatgcggc gtttaatttg cggttgtttt tctttttatt ggtcagaaaa acagatgttt
    14461 tagtttccaa cgatttggac acactattag ctaattattc ggcaaaatta ttcaaaccca
    14521 aaacgcggtt agtgtatgat tcgcatgaat attttacaga agtccccgaa ttacaaggtc
    14581 gatttgccaa aaaagtatgg gaaaaaatag aggcgtttat tttcccgaag ttaactacga
    14641 tttatactgt taacacttcc attgctgatt tgtaccagaa aaaatatggt aaaaaattgt
    14701 atgttgttcg caatgtatcg cccctttggc aagcaactaa tttgttgagc aaaaaggaat
    14761 tgaacttgcc agaagataag ccattaatca tcttgcaagg agctggaatt aatattgata
    14821 gaggaggaga ggaagcagtt gctgccatgc aatacattga aaaagcgtat ttgattattg
    14881 ttggagacgg cgatgtagtg cctcaattaa aagaatatgt tcaaaaatac cagttgagcc
    14941 acaaggtttt cttttatgga aaacaaccct atttgcgcat gatgaattat acctttcacg
    15001 caagtattgg cttaacattg gataaagaca ccaatatcaa ttaccgttat tcgttgccca
    15061 ataaattgtt tgattacatt cacacccata cagctgtagt gtgcacgcct ttagtagaaa
    15121 taaaacgtat tgttgaacac tatcaagtgg gtgaagtgtt agagcaatta acaccacaaa
    15181 tccttgctca aaaaatcaat tcattattgg aaaaccccgt gctattaaaa catttccaag
    15241 ataattgttt ggcagccaaa gaacaactca attggcaaaa cgaaacagct gttttaaagc
    15301 aaatttatgt tgaagaaatt gacagcaaaa ggaataaaaa ctaaaacatt gagctattgt
    15361 gattgaaaaa aacaaaattg gtttatcaat attgatccca gtatataatt atcccataaa
    15421 cgtattgtgg aatagtttag ttgctgtttt gcagtctttt aataatccgt atgaaataat
    15481 atttttagat gattgttcaa ctgatgttgc aatttataaa gcgaataaag ctttagttaa
    15541 aaatgaaagt aacgttgttt ttgaacaagc agattataat gttggacgag caacaattcg
    15601 taatagattg gcagaaaaag ctcaatacag ttatttgctt tttattgatg tagactcaaa
    15661 aataaacaac tctaattttt taacctcata ttggagtaat agaaatacag ctaaagtaat
    15721 tgtaggaggc acacactatt cgacatgtcc aaacccttca gttagtctac gcttttatta
    15781 tggaaaagcc agagaggagg tgttggcatc aaaacgaaat ataaaaccgt atgattcgtt
    15841 ggcgttaaat aatgttttca ttgagcgaca aacattttta gaacataagc ttgatgaaag
    15901 tataaaaaac tatggtcatg aagatacaaa atttggcctt gaattaaaag aaaaaaacat
    15961 ctctatattg catatagata attctgtaga gcacattggt ttagaagaaa acaaagtgtt
    16021 tttaggtaaa acaattaaag gcgttgaaaa ttttgcacta ttagttcaac aaggatacgc
    16081 tcatgatagt aaactatata aaacctactt tctgctcaaa aggctttatt tacttaagct
    16141 attcaatttt ttatattctt ttacatcaaa aataatatct aataatttaa tttcttcaag
    16201 accatcaatg gtttgttatg atgtttttaa gttatatcat tttattaaaa agatgcattt
    16261 agagcaataa ttatataatt cgatttttcg tttatcggca tttaataatt taatgtgtaa
    16321 tatcaaaatt gatgttgtag aataagaaca atcaattaaa ttcggttttt ttcaaaaaaa
    16381 atttttttta ctaaacgtga ttagtaaacc atttaggaca tttttatcta aaatagtttt
    16441 aaaaattgca cttaatcgtt atactaaaac gatatagttt ttctatattt gttccatctt
    16501 aaaaaatgtt ttatgaaaac acactacttt atcgctatat gcatactatg ttgcttagga
    16561 ggattacaaa atgtattttc ccagatttat gagattgatg cttataatgg acaaactata
    16621 aatttaacga ccattccttg cggaagtaag ttttacgaca gtggtggttc ggcaggcaat
    16681 tatttggcca atgaaaatca tactgtatcg ttttatagcg gagctgcttg taagcaagtg
    16741 gttgtaaaat ttaattctat atatttagaa aataattatg ataaactatt tgtttatgat
    16801 ggaccaactg atttaagtcc attaattcaa atactaacag gtaacagttt tagtggaaca
    16861 attgtatcaa cctctaacta tttaacattt aagtttacct ctgatgtgag tattcaatac
    16921 gcaggctggg atgcagatat tatttcttgc cccacattga gctatgaatc tggttgtgca
    16981 gttgctgctc ctttattggc caactcaaat ggtgtttttt cattttcaac acctccaggt
    17041 gatggagcag taataaatag cacaacaggt gttatcacca acggaacaca aggagcaacc
    17101 tatcaagtac acgatgcgtg ttggaattta aacgcaacgg ttacaatgag taacactcct
    17161 ccatgctaca acatgaatgg agatgcacaa gttttaactg ttggaggaaa cacttgtgtt
    17221 cagcttacac ctgaagcaaa cactaagact ggttgtgcat ggagtgaaca aaaagtggat
    17281 tttaactcca attttacatt gtccttagat tactattttg gtgataatcc tggaggagca
    17341 gatggtacaa cttttacgtt tcaacctaat ccaattgcat cgtgtggaca aaatggagac
    17401 caacttggcg ctggaggaat tcccaatgca ttaattgttg agtttgatac ttatgataat
    17461 gatggtgcag caaatagtga tttggcttgt gacaacatag ctgtggaaac g
//
LOCUS       DAOQVQ010000005        17027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_3836_length_17027_cov_4.272331, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000005 DAOQVQ010000000
VERSION     DAOQVQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 17027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17027
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_17027_cov_4.272331"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKN22_00400"
     CDS             complement(<1..336)
                     /locus_tag="PKN22_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNS41189.1"
                     /translation="MELNNLTAISPIDGRYHDKTTELQPYFSEFGLIKYRVIVEVEYL
                     IALVESGIEPLKNFPKDKFAQLREIVTNFSENDALWIKNTEKTTNHDVKAVEYFRKIS
                     YNFSQLRKLI"
     gene            479..1126
                     /locus_tag="PKN22_00405"
     CDS             479..1126
                     /locus_tag="PKN22_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS41190.1"
                     /translation="MKLHVAHFTFNPFQENTYLVYADNGDAVVIDPGCYEKHEEQALD
                     AFISTNNLTVLAVLNTHAHIDHVLGNAHVIDKYKVDYSLHPLDNSTLNAVTNYAHVYG
                     FPNYKTSPQPTKELKHGETLTFGAISFDVFFTPGHAPGHVVFYNKENNFVINGDVLFQ
                     GSFGRVDLPGGDLETLKTSIFETMFQLPEETVVYCGHGPATTIGKEKQTNYILNF"
     gene            1167..2237
                     /locus_tag="PKN22_00410"
     CDS             1167..2237
                     /locus_tag="PKN22_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNS41191.1"
                     /translation="MEGFGWYTYEISKRLVENHPEHQFFFFFDRPCDPRYVFAKNVTP
                     VVLRPPARHPILFYIWFELAVKKALNKYKIDLFFSPDGYLSLSSNVPQIGVIHDLNFE
                     HNPQDLPKSALVYLKHFFPKFAQKATQIITVSEYSKNDIVQLYGIEPQKIHAIWNGAS
                     EKYHPLTTEQQQAVRLKYTQGAHYFLYVGSIHPRKNLKRLVEAFVRFKKQTQSPTKLV
                     IVGAALWSNESFSSLIPTELQQEIVFTGHLALEELTQIVGAAKLLTYIPYFEGFGIPL
                     VEAMKCGVPLLSGNLTSLPEVAGNAAEYCNPFDVDEITQKLVDLDQNTHRLTELSQLS
                     LERASQFSWDKAAVEVARVLGL"
     gene            complement(2289..3248)
                     /locus_tag="PKN22_00415"
     CDS             complement(2289..3248)
                     /locus_tag="PKN22_00415"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNS41192.1"
                     /translation="MKRMIKYFGIATLVLAFSTSCDKVKKIYEPSDYDTDLNVALYPG
                     DWQTYLNTQWPNFDTITASSNRNILIDDFTGHNCQYCPDAAVAAHALHTQYPDRVFVS
                     SIHASPTGITGFQATNSQYPVDFTNEMGLETGKFFGGLATSGFFGNPSVSTSRIVVSG
                     GTEMFYPPGILANQVNSALATTLKVKIKSKVNYYAQTKGAFLHTEVEVLDNALTNLGM
                     IAVLHQDSLVAPQNVNGTYTPNYVHRDIHRGNVGNTLWGTTLTDALKDDNGKYYLNYS
                     FVVPNEIMLDGSEHFNAANMHLLIYVYDKTTYEIYQVVKEKFE"
     gene            complement(3268..4971)
                     /locus_tag="PKN22_00420"
     CDS             complement(3268..4971)
                     /locus_tag="PKN22_00420"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNS41193.1"
                     /translation="MRKLSIICSLLLLSTTTYSQLERGNFTGNLETTFQYLNKDSLIG
                     ATQPPSKALLNSYMNVYYTHGNFRGGLRFESYLPRIQGYPTNFQGTGLGMRYVGYANH
                     FIDATVGNFYEQFGAGLTLRAYEDRALGYDNMLDGVRVKLMPFRGIVVKGVYGLQRLG
                     FNGGISHSSGVVRGGDAEFNLSQIFPKIAEKEVDFMIGGSFVSKFQEDNDDLLILPQN
                     VATYGGRMALNYKGFTLNGEYVVKGQDPSQDNGFIYNPGFATVINAGYSRKGLGILFS
                     AKSVDNMSFRSDRTKVLQVGLINYLPALNKTHTYNLVASLYPYATQPTGEVAFQGDIV
                     YTIPKGSKIGGKYGTTISLNASTAFRPKRTPNLNYPGDSTGVAYSTSPFRMSDSLYWL
                     DINASIYRKFTKNFSASLSYFHLALNNNVAAVTKQPGVIRSNTFVFEFLYKFKKQSSL
                     RAELQVMLVNRIDSLNNRVPFNTKGAKPTDHGDWATLMVEYAINSHWMINVMDQYNFG
                     NPKKSSRAHHPYITVAYVTGPTRVSVGYGRQRAGMFCVGGVCRYVPASNGLTLSVTHS
                     F"
     gene            complement(4983..5495)
                     /locus_tag="PKN22_00425"
     CDS             complement(4983..5495)
                     /locus_tag="PKN22_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS41194.1"
                     /translation="MKKIGLLFIACAVVFSSFSQEKHESNKDKKLPVVQLKTMTGELV
                     NTSELTANGPVIISFWATWCAPCKRELNTISDVYDDWKDEFGVTLVAVSIDDEKTKGQ
                     VPTYVNGRAWDYLVVMDPNSDFKRAMGVNNVPHTFLVDKDGVIVYSHNNYTPGDEDVL
                     YQELVKLKQK"
     gene            5744..6523
                     /locus_tag="PKN22_00430"
     CDS             5744..6523
                     /locus_tag="PKN22_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41195.1"
                     /translation="MKKILLFGFGLMTWAAFAQVEIRNVGETTNLDGQTLTVTIPTSE
                     VDPSDEYAYWAHTKFHVYNLSGGDLQMRLRRDVVTAATDWVDQICWPPTCFPTNQVAT
                     YLTPHSNPSNPAPIVYDGGTTTDKNSGSPAEIKPQIYPKHPGSTATYKYTVVDVNNAT
                     EYASVTVIYNYIDDASVKAVKNTEITFSPNPASDAVTIQAEAITNGTIQIVDVLGNVV
                     YTSNFSGTKKINTSDMKNGVYIVSIQGDNAKGITKKLVVRH"
     gene            6578..7420
                     /gene="cysQ"
                     /locus_tag="PKN22_00435"
     CDS             6578..7420
                     /gene="cysQ"
                     /locus_tag="PKN22_00435"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNS41196.1"
                     /translation="MNFILTFGLLIEMKELNNHYTIALKASVLAADEIMRVYHTSFQK
                     ELKSDGSPVTEADKRASAIITKELKQTEIPIISEEELKENFEIRQSWKTVWIVDPLDG
                     TKEFIKQNDEFAVNIALVENGNPVFGLIASPVEQTILFGGKEIGCFIAPFNAIDSPNN
                     WTKLTAKELNNPVVLTSSRTPNFELFSPLIEEIKSKKDIDFIKKGSALKFFDLAQGWA
                     DVYPRFAPTMEWDIAAGQAILEALGGKVLDVNTGKPLTYNKESLYNPHFIAKTKAFIN
                     CFGE"
     gene            7417..8982
                     /locus_tag="PKN22_00440"
     CDS             7417..8982
                     /locus_tag="PKN22_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41197.1"
                     /translation="MMKRLKNSIALGIAICWTFALLAQQNNIPLNPFYKDRLYYSTSK
                     MEGYTGNSFFPVSEGQYDLHTKIQDTSKQYYLVTEHLFKKHFLEFKGKNYYLTISPVF
                     DVSMGKDFADTVKRWLFQNTKGFHIEGDLFKNFSFSTSFYENQSRNPIYQKNYFNAIG
                     ESYVKTSDSTYFTQNAVVPGLGRTKPFKVDGFDYAFAMGEIIYRPFKVLTLSAGNTRK
                     FVGDGYRSLLLSDNSFSAPFFQANYRFHPRWEFVYMRSKLLNMLRRPASTTVESYYDP
                     KILAVNYLSFKATKTLTISLFEGTIYSKGDSVVSKHASPWMYNPIPGLGAFVVKEKEA
                     NSILGLNIGYTIVSELRMYAQVAWNPRHKGYGAQLGFRISEPGHVKNLFLQLEGNYAS
                     TNLYRSSNPRLNYSHFNLPLAHTKGEGFAEIILRANYEIKRVYFDVKAIYYNVDKYSP
                     TSHLALYDKGSLHYGSTFYTSVETGYRFNKKMNFSVFINYLFRKDNTASPQLTNVFGI
                     GMRTGLINSYTDF"
     gene            8979..10415
                     /locus_tag="PKN22_00445"
     CDS             8979..10415
                     /locus_tag="PKN22_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41198.1"
                     /translation="MKTLGIHIVFVLLTTIVWGQYGTGSIYEELPQDSVAKPSVEVHT
                     SILPAARQYTFASWKKNDSIRKNYYFIAPAVDINGGYAKQMAYRTGLGLNFHAQVGKW
                     AFKIGAFGGIGSVDSLFNTPAFYYERKGKNYYYADIRGRVSYTPNEIFSFHVGLDNNF
                     IGEGSRSLFLSDYGAPHPFAQVKAKFWRVEYDLFYQFMREHSSKGWRSKFATTHHISL
                     NATKWLNFGLFETVLFQPKDTLLNRGYEAEYLNPLVFFRPQEYAIGSADNILLGVSMN
                     ARYKGHVFYFQFLLDEFSLKEIKNNPSWWANKYGAQFGFKGRFNTKIGPFFYRAEYNF
                     MRPYTYAHLNEMQAYGNQGHVLAHPLGGNFHELVAELKWQKGKYMAKFFTTYRLKGED
                     KDGFSYGGDIYMPYTLRPSEYGHKIGQGAGKNVSKTILTFDYEVWKAGQIHVFFENHL
                     WIQANKMQVTYIPVIGIRSQLWNDYRNY"
     gene            complement(10423..11388)
                     /locus_tag="PKN22_00450"
     CDS             complement(10423..11388)
                     /locus_tag="PKN22_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNS41199.1"
                     /translation="MTSTNFEFVPYTSAFLSELTATRAGETKLGEKLQVGLNSTAKFV
                     LIGVQEDIGPQANLGKPGATNAFPAFLSRFVNMQSNRFLSGDIIAIGGFVKQTTFFST
                     VEEGKLLVDELDNMLIQLLTPLYQNNQIPILIGGGHNNAYALIAAYNKAKAKSLEVIN
                     LDPHADCRRLEGRHSGNSFSYAQENGFLTTYSVLGLHKAYNGENMLSYLDEQQFFYSF
                     FEEYLLAPEKLTVDLLDVLEKNDEVLGVELDLDCMKNMPSSAFTPSGLEVEQARKYLT
                     KIARSGRQVAYVHLPEGAPITSDDEKIVGKTLAYLVHDFIANYSI"
     gene            complement(11385..11801)
                     /locus_tag="PKN22_00455"
     CDS             complement(11385..11801)
                     /locus_tag="PKN22_00455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNS41200.1"
                     /translation="MEFSFKNTTKLRVRYAETDQMGYVYYGNYAQYFEVGRVEAMRSV
                     GMSYRELEEQGIMLPVSEFSTNYFAPAKYDDELTITTSIVAVKGARLQFDYEIHNDQG
                     TLIVKASTTLVFVAKNTMRPIKAPDLFLKLLEKYSV"
     gene            12148..13578
                     /gene="dnaA"
                     /locus_tag="PKN22_00460"
     CDS             12148..13578
                     /gene="dnaA"
                     /locus_tag="PKN22_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNS41201.1"
                     /translation="MNNTHAAVWESCLKVIKDNVSLQAYRTWFDPIIPVKLENSVLTI
                     QVPSHFFYEWLEENYITLLKKVIHKELGSEGRLEYSIIMENNSTNSTPYTVKLPALNK
                     KTLKNAPVTMPLSVGENQIRNPFVIPGLKKIHVESNLNPACSFDNFIEGDCNRLARAS
                     GYAVANKPGGTAFNPLLIYGGVGLGKTHLAHAIGIEIKNQFPNKTVLYVGSEKFAHQF
                     IDALGNNTTNDFIHFYQMIDVLIIDDVQFFSGKEKTQDVFFHIFNHLHQNGKQIILTS
                     DKPPVEMQNMEQRLLSRFKWGLSADLSTPDLETRIAILEKKMYGNGIELPREVVEYLA
                     YSINTNVREMEGALNTLLAQASLNRKAITLELAKQMVDKFVKNTAREVSIEYIQKIVC
                     DYFDMPIEMLKSKTRKREVVQARQIAMYFSKKMTKSSLANIGANCGGKDHATVLHACR
                     TVINLAETDKQFKAYLEDLEKKLTIQ"
     gene            13585..14520
                     /locus_tag="PKN22_00465"
     CDS             13585..14520
                     /locus_tag="PKN22_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNS41202.1"
                     /translation="MDQPVTIIGSMFGNIFWIFIGALLIFKSFIIVKEQTAVVIERLG
                     KFNRIANSGFGIIIPFIDRKAGLVNLRVQQLDVEIETKTKDDVFVHLQVSVQYQVANS
                     KIKEAYYSLDNPRNQIASYVFDDVRAEVPKLELDDVFARKEDIAIAVQQNIAESMQEY
                     GYLIIKALITDINPDAKVKESMNRINAAKREKEAALEEGEAKKIKIIKEAEAEAESKR
                     LSGEGIAQQRLEIVRGFKESVEDFKRSMDNVTHEEIMQFVLMTQYFDTIKDIGANSKN
                     SSILIPHSPGGLKEFRDQIIQGTYIGKEMGEQTND"
     gene            14637..16481
                     /locus_tag="PKN22_00470"
     CDS             14637..16481
                     /locus_tag="PKN22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS41203.1"
                     /translation="MKSIREIYQHTFKHKGLALLVIFYNLLFVIFNLVSMVLFIPFLQ
                     LIFPNDTQAVVVPQKPVFNGGFNGTIQYVSDYYNYFTQSMVAENPKNALLFVCFSVLI
                     AFLLKNLFRYMAIWHQSQLRMVVVRDVRQSLFDKAMSLPLSFYSNERKGDLMSRIQSD
                     VGEIEIAVIAVLELIFREPIAVIINIAALLYWSPQLTLFSFILLPITAFVISRIGKSL
                     KRTAGQERIELGVLFSKMEEALGGIRIIKAFNAQKFIKDSFAKTNLRHQQLTTKAFRK
                     RDLSPPLNEFLAVVVMLGIVWYGGSMILDNKGTNGFDGPQFIGFILVFSQLLRPIQGI
                     ATSIANLNKAHAAQDRINEILNADEKIHEVEHPVENKPLTTNVSYNDVCFSYNDEPVL
                     KNVSFTIPKGKTVALVGESGSGKSTISDLLPRFYDVVSGSISIDNTDIRELKITDLRD
                     KIGIVSQESILFNGTVAENIAFGKPNATRQEIIEAAKIANAHKFIEELENGYDTQIGE
                     RGNKLSGGQKQRVSIARAVLKNPEILILDEATSALDTESEKLVQEALNKLMAERTSLV
                     IAHRLSTIIHADEIIVLSKGEIIERGTHAELLAKGGTYAYLSSLQGIA"
     gene            complement(16478..>17027)
                     /locus_tag="PKN22_00475"
     CDS             complement(16478..>17027)
                     /locus_tag="PKN22_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS41204.1"
                     /translation="YFSVQQMEEILLAGKKYGLQPKVHVNQFSIMGGIQKAVELGALS
                     VDHLEEISQEDIDCLKNSTTIPTILPSCSHFISIPFGNARWMMENDLPVALASDFNPG
                     TTPSGNLGFVWSLACVKMKMTPEEALNALTINAAAAMGLSATHGRIALGRRSPIILTK
                     EIPSLAYIPYAFGDMHIERVII"
BASE COUNT         5527 a         3193 c         3072 g         5235 t
ORIGIN      
        1 gataagtttg cgcaattgcg agaaattgta actaattttt ctgaaatatt ccaccgcttt
       61 cacgtcatga ttggtggttt tctccgtatt ttttatccac aaagcatcgt tttcagaaaa
      121 attagttaca atttctcgca attgcgcaaa cttatctttt ggaaagtttt ttaacggctc
      181 aattcccgat tcaaccaagg caattaagta ttctacctcc acaattacgc ggtattttat
      241 caatccaaat tcagaaaaat acggttgtaa ttcggtagtt ttatcgtggt aacgtccatc
      301 aataggagaa attgccgtta aattatttaa ttccatactg ttataagttt taaaatctgt
      361 ttttctgttg tcaaaaaaag taagctacaa agatagtaaa ttatcgcaaa taaaagcaag
      421 cctaaattga aatctaaagc aagctggaaa tacaaaaaaa atgagttaac tttacactat
      481 gaaattacac gtagctcatt ttacatttaa tccttttcag gaaaatacat accttgttta
      541 tgccgataat ggcgatgccg tagttattga cccgggatgc tatgaaaaac acgaggaaca
      601 agcgttggat gccttcattt ctaccaacaa tctcactgtt ttggcagttt tgaacacaca
      661 cgcacatatt gatcacgtgt tgggaaatgc acatgttata gacaaataca aagtggatta
      721 ttccttgcat ccgcttgata attcaacatt aaatgcggtg accaattatg ctcacgttta
      781 tggttttccg aattacaaaa catctccaca accaaccaaa gaattgaaac atggagaaac
      841 cttaactttt ggagctattt catttgacgt gttttttacg cctggacatg cacctggaca
      901 cgttgttttt tacaacaagg aaaacaattt tgtaatcaat ggcgatgtgt tgtttcaagg
      961 tagttttggt cgtgtggatt tgcctggtgg tgatttggaa acactgaaaa cttctatttt
     1021 tgaaaccatg tttcagcttc ccgaagaaac agttgtttat tgtggacatg ggcctgcaac
     1081 tacgatagga aaagaaaaac agaccaatta tattttgaat ttttaaggtg cgcattgcca
     1141 taaatacacg gatgttgctt ccaaaaatgg aaggttttgg ttggtacacc tatgaaattt
     1201 ccaaacgatt ggtagaaaat catccagaac atcaattttt cttctttttt gaccgtccgt
     1261 gtgatcctcg atatgtgttt gcaaagaatg taacacctgt tgttttgcgc ccacctgcac
     1321 gtcatcccat cttattttat atttggtttg aattggctgt gaaaaaggca ttgaataaat
     1381 acaaaattga tttgtttttt tcacccgacg gctatttatc tttgagttcc aatgttccac
     1441 aaattggtgt gattcacgat ttgaattttg aacacaaccc gcaagattta cccaaaagtg
     1501 cgctggttta tttgaaacat ttttttccaa aatttgctca aaaggcaacg caaattatta
     1561 ctgtttctga atattccaaa aacgatattg tgcaacttta tggcattgaa ccacaaaaaa
     1621 ttcatgctat ttggaacggc gcatcggaaa aataccaccc actaacgaca gaacaacaac
     1681 aagctgttcg tttaaaatac acacaaggtg cacactattt tttatacgtt ggttccattc
     1741 atccacgaaa aaatctcaaa cgtttggttg aagcttttgt acgattcaaa aagcaaacgc
     1801 aatcgcctac aaaattggtg attgttggag cagcattgtg gagcaacgag tcgttttctt
     1861 cgttaattcc aaccgaattg caacaagaaa ttgtttttac aggacattta gcccttgagg
     1921 aattgaccca aattgtgggt gctgctaaat tattgaccta catcccctat ttcgaaggat
     1981 ttggaattcc gttggtagaa gccatgaaat gtggcgttcc gttgttatcg ggcaatttaa
     2041 cgtcgcttcc agaagttgca ggcaatgcgg cagaatattg caaccctttt gatgtggatg
     2101 aaatcacaca aaaactggta gatttagacc aaaacacgca tcgtttgaca gaattgagtc
     2161 aattaagttt ggaacgtgct tcgcaattta gttgggacaa agccgctgtg gaagttgcac
     2221 gtgttttagg gctataaaaa aagctgtcca ataaatagac agcttttaca gttccgtttt
     2281 caggtgtttt attcaaattt ttcttttacc acttgataaa tttcgtaggt agttttatca
     2341 tacacataaa tcaacaagtg catattggct gcattaaaat gttcgctacc atccaacata
     2401 atctcgttgg gaacaacaaa actataattc aaataatact tgccattatc atctttaaga
     2461 gcatctgtca acgtagttcc ccacaacgta tttccaacat ttcctctgtg aatatcgcga
     2521 tgtacatagt tgggtgtata agttccatta acattttgag gtgctaccaa actatcttgg
     2581 tgcaacactg caatcattcc taaattggtc aatgcattat ccaatacctc aacctctgta
     2641 tgcaaaaatg cacctttggt ttgagcataa taattcacct ttgatttaat ttttactttc
     2701 aacgttgttg ccaaagcaga atttacttga ttagccaaaa ttcccggagg ataaaacatt
     2761 tctgttccac cacttaccac tatgcggctg gtgctcacac ttggattacc aaagaaacca
     2821 cttgtagcta aacctccaaa aaacttacca gtttccaacc ccatttcgtt ggtaaaatca
     2881 actggatatt gtgaattggt tgcttgaaaa cctgtaattc ctgtaggaga agcatgaata
     2941 gaactcacaa aaacacgatc gggatattgc gtatgcaatg catgagcggc aactgctgca
     3001 tctggacaat attggcaatt gtgtcccgta aaatcatcta tcaaaatatt gcggtttgat
     3061 gacgctgtta tagtatcaaa attaggccat tgtgtgttta agtaggtttg ccaatcgcca
     3121 ggatacaacg caacattcaa atcggtatcg taatcactgg gctcatagat tttttttact
     3181 ttatcacagc ttgttgaaaa tgctaacacc aaggttgcaa ttccaaaata ttttatcatt
     3241 cgtttcataa ttgttgcttt atttcaatta aaaactatgt gttaccgaca aagtcaaccc
     3301 gttggatgca ggcacataac gacaaacacc accaacacag aacataccag cacgttgacg
     3361 accataacca acagaaacac gtgttggtcc cgttacatac gccactgtga tatacgggtg
     3421 gtgtgcgcgt gaactctttt taggattccc aaaattatat tgatccatca catttatcat
     3481 ccaatgagaa ttaatggcat actctaccat caaggttgcc caatcaccat gatcggttgg
     3541 ttttgcacct tttgtgttaa acggcacacg gttgttcaaa gaatcgatgc ggtttaccaa
     3601 cataacttgt aattctgctc gcaacgacga ttgtttttta aacttataca agaattcaaa
     3661 aacaaatgta ttggagcgaa ttacccccgg ttgcttggtt actgcagcaa cattattgtt
     3721 caacgctaaa tggaaatagc tcaaggaagc actgaaattc ttagtaaact tacggtaaat
     3781 acttgcattg atatccaacc aatacaaaga atcactcatt ctaaatggag aggttgaata
     3841 ggcaactcct gttgaatctc ccggatagtt aagatttgga gttcgttttg gtctgaacgc
     3901 tgtagaggca ttcaaactga tggttgttcc atattttcct ccaattttac ttccttttgg
     3961 aatggtataa acgatatcac cttgaaatgc tacctcacca gttggttgcg ttgcataggg
     4021 atacaaagaa gcaactaagt tataggtatg tgttttattc aatgctggca aataattgat
     4081 taaacctact tgcaatactt tagtgcgatc ggaacgaaaa ctcatattat ctaccgattt
     4141 ggcagaaaat aaaatcccta atcctttgcg ggaataccca gcattgataa ccgtagcaaa
     4201 accaggattg taaataaaac cattgtcttg tgaggggtct tgacctttta ctacatattc
     4261 tccattcaac gtaaaacctt tgtagttcaa cgccattcta ccaccatagg tagccacgtt
     4321 ttgaggtaaa atcaacaaat catcgttgtc ttcttggaat ttacttacaa acgaaccacc
     4381 aatcatgaaa tccacttctt tttcggcaat ttttggaaag atttgactca aattaaattc
     4441 ggcatctcca cctctaacaa caccactgct atgtgatatt ccaccattga atcccaatcg
     4501 ttgcaatcca taaacacctt ttacaacaat accgcgaaaa ggcattagtt ttacacgcac
     4561 accatccaac atgttgtcat aacctaacgc tctgtcttcg taggcacgca aagttaaacc
     4621 agcaccaaat tgttcgtaaa agttaccaac cgttgcatca ataaaatgat tagcatagcc
     4681 aacataacgc attcctaaac cagttccttg aaaattggtt ggataacctt gtatgcgtgg
     4741 caagtacgac tcaaaacgca atcctcctct aaaatttccg tgtgtgtaat acacgttcat
     4801 gtaggaattc aacaacgctt ttgaaggcgg ttgtgtagct ccaattaacg aatccttgtt
     4861 caaatattga aaagtcgttt ctaaattacc tgtaaaattc cccctttcta attgagaata
     4921 tgttgtagta cttaagagta ataaagaaca aataatagaa agttttctca tttaaaattg
     4981 atttattttt gtttcaattt caccaattct tggtacaaca catcttcgtc gcctggtgta
     5041 taattgttgt gtgagtaaac gattacgccg tctttatcaa ctaaaaatgt atgaggtacg
     5101 ttgtttacac ccatcgcgcg tttaaaatcg ctgtttgggt ccatcaccac taaataatcc
     5161 catgctctac catttacata agtaggcact tgaccttttg ttttttcatc atcaatagaa
     5221 acggcaacca aggtaacacc aaattcatct ttccaatcgt catacacatc actaatggtg
     5281 tttaactcgc gtttacatgg tgcgcaccaa gtagcccaaa agctaataat tactggtcca
     5341 ttagcagtta gttcacttgt gttaaccaac tcacctgtca ttgtttttaa ctgaacaaca
     5401 ggcaattttt tgtccttgtt agactcgtgc ttttcttgag aaaaagaaga aaaaacaaca
     5461 gcacaagcaa taaacaataa tccaattttt ttcatttttt tgagatttta tagtttcatt
     5521 tagctctggc aaacaccttg ccaataactc caaagataga atagaatttt tacactttat
     5581 cgttttattt tagaaaattc taacaccctc ttttggaatc ctaaaatgag acaacactaa
     5641 aattttgtta aatcagcaaa taatcatttg attttttttg tatttttacg aaatagtttt
     5701 ggatagattt ttgctattca tttataaaac aacacaaatt gatatgaaaa aaatattact
     5761 ttttggattt ggattaatga cttgggctgc ttttgcacaa gtggagataa gaaacgttgg
     5821 tgaaactacc aatttagacg gacaaacatt aacagttact attcctacct ctgaagtgga
     5881 tccaagtgat gaatatgcgt attgggcgca tactaaattt cacgtttaca atttaagtgg
     5941 tggtgatttg caaatgcgtt taagacgtga tgttgttaca gctgcaacag attgggtaga
     6001 tcagatttgc tggcctccta catgtttccc aactaatcaa gtagcaacgt atttaacgcc
     6061 tcatagtaac ccttcaaacc cagctcctat agtatatgat ggtggaacaa ctactgataa
     6121 aaacagtggt tctccagcag aaataaaacc tcaaatttat ccaaaacacc caggaagcac
     6181 tgcaacttac aaatatacgg ttgtagatgt taacaacgct acagaatatg cttctgttac
     6241 cgtaatctac aattatattg atgatgcaag tgtaaaagct gtaaaaaaca cagaaattac
     6301 gttttctccc aacccagctt ctgatgctgt aaccattcaa gcggaggcaa tcaccaatgg
     6361 aaccattcaa attgtggatg tgttaggaaa tgtggtgtac acaagcaatt tctctggaac
     6421 taaaaaaatc aatacttctg atatgaaaaa tggtgtttac attgtttcta ttcaaggaga
     6481 taacgcaaaa ggaattacaa aaaaattggt tgtaagacat taataagaca caacctgcaa
     6541 ttattttaga ccgctttttt gaaaggcggt cttttttttg aattttatac ttacatttgg
     6601 gctacttatt gaaatgaaag aattgaacaa tcactatacc attgcactta aagcgtccgt
     6661 tcttgcagca gacgaaataa tgcgtgtgta tcatacttcg tttcaaaaag aacttaaatc
     6721 agatggatcg cctgttactg aggccgacaa acgagcttct gcaataatca ccaaagaatt
     6781 aaagcaaaca gaaattccaa ttatcagcga agaagaactg aaggaaaatt ttgaaattcg
     6841 tcagagctgg aaaacggttt ggattgtaga tccgctagat ggaaccaagg aattcattaa
     6901 acaaaatgat gaatttgcag taaacattgc attggttgaa aacggtaatc ctgtttttgg
     6961 attaattgca agtcccgttg aacaaacaat tttatttgga ggaaaagaaa ttgggtgttt
     7021 tattgcacca tttaatgcta ttgattcgcc taacaattgg acaaaattaa ctgccaaaga
     7081 gttgaacaat cctgtagtat taaccagttc gcgcacacct aattttgagt tattttctcc
     7141 tctaattgag gaaatcaaaa gtaaaaaaga catcgatttt atcaaaaaag gatcagcctt
     7201 gaagtttttt gacctcgcac aaggatgggc agatgtttac ccacgctttg caccaactat
     7261 ggaatgggat atagctgctg gtcaagcaat tttagaagcg ttgggaggta aagtgcttga
     7321 cgtgaatact ggaaaaccgc taacctacaa caaagaatcg ttgtacaatc cgcattttat
     7381 tgcaaaaaca aaagcgttta ttaattgttt tggagaatga tgaagcggtt aaaaaatagc
     7441 atcgctcttg gtattgccat ttgttggact tttgctctat tggctcaaca aaacaacatt
     7501 ccgctaaatc ctttttacaa agatcgtttg tattattcca cctctaaaat ggaaggctat
     7561 acaggcaata gcttctttcc tgtaagcgaa ggacagtatg atttgcacac caaaattcaa
     7621 gatacttcta aacaatatta tttggtaacc gaacatttat tcaaaaagca tttcttggaa
     7681 tttaaaggta aaaattacta cctcaccatt tcaccagtat ttgatgtatc aatgggaaaa
     7741 gattttgccg ataccgtaaa acgttggtta tttcaaaaca ccaaaggttt tcatattgaa
     7801 ggcgatttat tcaaaaactt ttcgttttct accagttttt acgaaaatca atcgcgcaat
     7861 cccatctacc aaaaaaatta tttcaatgcc attggtgagt cgtatgtaaa aacatctgat
     7921 tccacttatt ttacccaaaa tgctgttgtg ccggggcttg gacgaacaaa acctttcaaa
     7981 gtggatggtt ttgattatgc gtttgctatg ggtgaaatca tatatcgacc atttaaagta
     8041 ttgacacttt cggcaggaaa tacccgaaaa tttgtgggcg atggctaccg ttcactgttg
     8101 ctttctgaca atagttttag cgcaccattt tttcaagcca attatcgttt tcatccacga
     8161 tgggagtttg tttacatgcg tagtaaattg ctcaacatgt tgcgtcgacc tgccagtaca
     8221 acggttgaaa gctattacga cccgaaaatt ttagcggtga attacctttc gtttaaagcc
     8281 accaaaacat taaccatctc gctttttgaa ggaacaattt attcaaaagg tgattcagtg
     8341 gtttccaaac atgcttcgcc gtggatgtat aaccctattc caggacttgg tgcctttgtt
     8401 gtgaaagaaa aagaagccaa ttcaatttta ggattaaaca ttggctacac cattgtttca
     8461 gaacttagaa tgtatgcaca agttgcatgg aatccacgtc acaagggata tggcgcacaa
     8521 ttaggtttta gaatttcaga acctgggcat gttaaaaacc tctttttaca gttggaagga
     8581 aactatgctt ctactaattt ataccgttct tccaatccgc ggttaaacta cagccatttc
     8641 aatcttccat tggcacatac caaaggtgaa ggttttgcag aaattatact gagagctaat
     8701 tacgaaatca aacgggttta ttttgatgta aaagctattt attacaacgt tgataaatat
     8761 tcacctactt cgcatttagc cttgtatgac aaaggctcgc ttcattatgg aagcactttt
     8821 tacaccagcg tagaaacagg ttatcgtttt aataaaaaaa tgaatttttc ggtatttatt
     8881 aattatttat tccgaaaaga caacacagca tctcctcaat taaccaatgt atttggaatt
     8941 gggatgcgaa caggtttaat caatagctac actgattttt gaaaacacta ggcatacata
     9001 tcgtttttgt tttgttgacc acaatcgttt ggggacaata tggcactggg tcaatatacg
     9061 aagaattgcc tcaagacagt gtggccaaac cttcggttga agtacataca agcatacttc
     9121 cggcagcacg tcaatacact tttgcttcgt ggaaaaaaaa cgatagcatc cgcaaaaatt
     9181 attacttcat tgcgccagct gttgatatca atggtggata tgcaaaacaa atggcttatc
     9241 gcactggttt gggattaaat tttcatgcgc aagttggaaa atgggcattt aaaattggag
     9301 catttggtgg tattggttct gtagattctc ttttcaacac acctgcattt tattacgagc
     9361 gtaaaggcaa aaactactat tatgccgaca ttcgcggtcg cgtgagctat acaccgaatg
     9421 aaatttttag ttttcatgtt gggttggaca ataattttat tggagaagga agtcgctctt
     9481 tgtttcttag cgattacggc gcacctcatc catttgccca agtgaaagca aaattttggc
     9541 gtgttgaata cgatcttttt tatcaattca tgcgtgagca ctcatcaaaa ggatggcgat
     9601 ctaagtttgc aactacgcac cacattagtt taaacgctac taaatggctc aacttcggat
     9661 tgtttgaaac ggtgcttttt caacccaaag acaccttgct caaccgtggt tatgaagccg
     9721 aatatttgaa cccgcttgtg tttttccgtc cacaagaata cgccattggt agtgccgaca
     9781 acatactttt aggagtttca atgaatgcac gatacaaagg acatgttttt tattttcaat
     9841 tccttttgga cgagttcagc ttgaaagaaa ttaagaacaa tccatcgtgg tgggcaaata
     9901 aatatggtgc acaatttggt ttcaaaggcc gatttaacac caaaattgga ccgtttttct
     9961 atcgtgcaga atacaatttc atgcgtccct acacttatgc acacctcaac gaaatgcagg
    10021 cttatggcaa tcagggacat gtgttggcgc atccattggg aggtaatttc cacgaattgg
    10081 tagccgaatt aaaatggcaa aaaggaaaat acatggctaa atttttcacg acctatcgtt
    10141 taaaaggcga agacaaagat ggatttagtt atggtggcga tatttacatg ccttatacgc
    10201 ttcgtccgag tgaatatgga cataaaattg ggcaaggtgc aggcaaaaac gtaagtaaaa
    10261 ccatactaac gtttgattac gaagtgtgga aagccggaca aatacacgtg ttttttgaaa
    10321 atcacttatg gattcaagcc aacaaaatgc aagtaactta tattccagtg attggtattc
    10381 gcagccagtt gtggaacgac tatcgcaatt attaaacaaa gtttatatgg aataattggc
    10441 aataaaatcg tgtaccaaat aagccaatgt ttttccaact attttttcat catcggaagt
    10501 tattggagct ccctcgggta aatgcacata agcaacttgg cgtccagaac gggcaatttt
    10561 tgtgaggtat ttgcgtgctt gttccacttc caatccactt ggtgtaaaag cagaagacgg
    10621 catatttttc atacaatcca aatccaattc aacacccaaa acttcgtcgt ttttttccaa
    10681 tacatcgagc aaatccacgg ttaatttttc aggagcaagc aagtattctt caaaaaaaga
    10741 ataaaagaat tgttgttcat ccaaataact taacatgttt tcaccattgt aggctttgtg
    10801 caagcccaac accgaatagg tggttaaaaa accattttct tgtgcatacg aaaacgagtt
    10861 tccactatga cgcccttcca aacggcgaca atcggcatga ggatccaaat ttatcacttc
    10921 caacgatttt gcttttgcct tgttataggc cgcaatgagc gcataagcat tgttgtgtcc
    10981 accaccaatc aaaatcggaa tttgattatt ttgatagagc ggtgttaata gttgaatgag
    11041 catgttatcc aattcatcca ccaatagttt tccttcttca accgttgaga aaaaagtagt
    11101 ttgcttcaca aatccaccaa tagcaattat atctccactt aaaaaacggt tggattgcat
    11161 gtttacaaag cgggataaaa atgctggaaa agcgttggtt gcgccaggtt tacctaaatt
    11221 agcttgcgga ccaatatctt cttgcacgcc aatgagcaca aacttagccg ttgaatttaa
    11281 tccaacttga agtttttcac ccaattttgt ttctcctgct ctagttgctg ttaattcaga
    11341 caaaaaagca gaggtataag gaacaaattc aaaattagtg gaagtcatac gctatatttt
    11401 tcaagtaatt taagaaataa atctggtgct tttatggggc gcatggtgtt ttttgctaca
    11461 aaaacaagtg ttgttgatgc tttgacaatt aacgttcctt ggtcgttgtg tatttcataa
    11521 tcaaattgga ggcgtgcgcc tttaactgca acaatagatg tagtaatggt taattcatcg
    11581 tcgtatttag caggggcaaa ataattggtt gaaaactcag aaacgggcaa cataatacct
    11641 tgttcttcca attcacggta actcatccca acacttcgca tagcctcgac cctacccact
    11701 tcaaaatact gagcataatt accataatag acatatccca tttgatccgt ttcggcgtat
    11761 cgcacccgta attttgttgt atttttaaag gaaaactcca taatgtgtaa taaatatcaa
    11821 ttttaaaaaa gaaatcaatt gcttgagtgg aaaaaaaaca tatatttgaa gaatggattg
    11881 ttaataattg cattcacaaa atatttttta cttttttatt ttttaacaaa acactgaaaa
    11941 acaccattaa cacaaaggaa acaaatcaat taaaaaatta caattgtatt acaagtaaaa
    12001 aaaacgataa agtcaatagc aaatccattt ttttttaaac ttttttatgt aaatatttgt
    12061 agcactaaaa aaagaattaa tcacacaatt gctgaataca attgataaaa atataaatgt
    12121 ttaaccataa ctaactaaag acgctcaatg aataatacac atgctgccgt ttgggaatct
    12181 tgtttgaaag tcatcaagga caatgtatca ctgcaagcat atagaacatg gtttgatcct
    12241 atcattcctg tgaagcttga aaatagtgta cttacgatcc aggtgccttc acattttttc
    12301 tacgaatggt tagaagaaaa ctacattact ttattgaaaa aagttattca caaagaattg
    12361 ggaagcgagg gacgtttgga gtacagtatt attatggaaa ataattctac taattcaact
    12421 ccatacaccg taaaattacc cgctttgaac aaaaaaacac ttaaaaatgc gcctgtaacc
    12481 atgccattaa gtgttggaga aaatcaaatc agaaatccat ttgttattcc aggattaaag
    12541 aaaattcatg ttgaatcgaa cttaaatcct gcatgttcat ttgataattt tattgaagga
    12601 gattgcaacc gcttggcaag agcgtcgggt tatgctgttg caaataaacc tggtggcaca
    12661 gcatttaatc cacttttaat ttatggtggt gttggattgg gaaaaacaca cttggcacat
    12721 gccattggta ttgaaattaa aaatcaattc cctaacaaaa ctgttcttta tgttggttca
    12781 gaaaaatttg ctcaccaatt tattgatgcg ttaggaaata acacaaccaa tgatttcatt
    12841 catttttatc aaatgattga tgtattaatc attgatgatg tgcaattttt ctcaggaaaa
    12901 gaaaaaacac aagatgtttt cttccatatt ttcaatcact tacaccaaaa cggcaagcaa
    12961 atcattctta catctgataa accgccggta gaaatgcaaa acatggagca acgtttgctt
    13021 tcgcgtttca aatggggatt gtcggctgat ttgagtacac cagacttgga aactcgcatt
    13081 gccattttgg aaaagaaaat gtatggaaac ggcatcgaat taccacgtga agtagtagaa
    13141 tacttagcct attctatcaa caccaacgtt agagaaatgg aaggtgcttt aaacacattg
    13201 ttggcccaag catcgttgaa tagaaaagct attacattgg agttggcaaa acaaatggtg
    13261 gacaagtttg tgaaaaacac tgcacgtgaa gtttctattg aatacatcca aaaaattgtt
    13321 tgcgactatt tcgacatgcc aattgaaatg ttgaaatcta aaacaagaaa acgcgaagtg
    13381 gttcaagctc gccaaattgc gatgtatttc tctaagaaaa tgacaaaatc atcgttggca
    13441 aacattggtg caaactgcgg tggaaaagac cacgcaactg tattgcacgc atgcagaacg
    13501 gtaattaact tggctgaaac cgataagcaa tttaaagctt acttagaaga tttagaaaag
    13561 aaattaacca ttcaataatt tattatggat caacctgtta ctatcattgg aagtatgttt
    13621 ggcaacatct tttggatttt tattggtgcg cttttaattt tcaagagttt cattattgtt
    13681 aaagaacaaa ctgcagttgt catcgaacga ttgggtaagt ttaaccgcat tgccaactct
    13741 ggttttggaa ttatcattcc tttcattgat agaaaagctg gattggttaa tttgcgtgtg
    13801 caacaattgg acgttgaaat tgaaacaaaa acaaaagacg atgtgtttgt gcacctgcaa
    13861 gtttctgttc aataccaagt tgctaattca aaaattaaag aagcgtatta ttcgttggat
    13921 aatccacgca atcaaattgc ttcgtatgta tttgatgatg tacgtgccga ggttccaaaa
    13981 ttggagttag acgatgtttt tgcacgtaaa gaagatattg caattgcggt gcaacaaaac
    14041 attgccgaaa gcatgcaaga atatggctat ttaatcatca aagcgttgat aactgatatc
    14101 aatcctgatg ctaaagtaaa agaatccatg aaccgcatca atgctgcaaa acgtgaaaaa
    14161 gaagcagctt tggaagaagg agaggctaaa aaaattaaaa tcatcaagga ggcggaagct
    14221 gaggctgaat caaaacgctt atcgggtgaa ggtattgcac aacaacgttt ggaaattgta
    14281 agaggtttca aagaatctgt tgaagatttt aaacgttcta tggacaacgt aacccacgaa
    14341 gaaattatgc aatttgtatt gatgacccaa tattttgaca ccatcaaaga tattggagcc
    14401 aactctaaaa attcttctat cttaattcct cattcaccag gtggattgaa agaatttaga
    14461 gatcaaatca ttcaaggaac ttatattgga aaagaaatgg gtgagcaaac aaatgattaa
    14521 tcattctatc aaaataatta aaaatcggtt ttactaagtg gagccgattt tttttgtttt
    14581 tatccaaaaa ttttaaaaaa cacgttcgtt gtttcatttt aattaacttt acggcaatga
    14641 aaagcatccg agaaatatac caacatacgt ttaaacacaa aggactagct cttttagtta
    14701 ttttctacaa cttgctgttt gtgattttca atttggtatc gatggtgttg tttattccat
    14761 ttttacaatt gattttccct aacgatacac aagctgttgt tgtgccacaa aaacctgttt
    14821 tcaatggagg atttaatggt acaatacaat acgtttctga ttactacaat tattttaccc
    14881 aaagtatggt ggccgaaaac cctaaaaatg cgttattgtt tgtttgtttt tcggtattga
    14941 ttgctttttt attgaaaaac ctatttcgct atatggcgat atggcatcaa tcgcaattgc
    15001 gcatggtagt tgtgcgtgat gtacgccaaa gtttgtttga taaagccatg tcgttgcccc
    15061 tatcgtttta tagcaatgaa cgaaaaggcg atttaatgtc gcgtattcaa agtgacgttg
    15121 gtgaaataga aattgccgta attgctgttt tggagttaat ttttagagaa cccattgcag
    15181 tgattattaa cattgctgcg ttgttgtatt ggagtccaca gttaaccttg ttttcgttca
    15241 ttttattgcc catcacagca tttgtaattt ctcgcatcgg caaaagcttg aaacgaactg
    15301 caggacaaga acgtattgaa ttgggagtgc tgttctccaa aatggaagaa gcattgggtg
    15361 gcattcgcat catcaaagca tttaatgctc aaaaattcat caaagattcg tttgctaaaa
    15421 ccaacttgcg ccaccaacaa ttaaccacca aagcatttcg taaacgcgat ttatcgccac
    15481 cgttgaatga atttttagcc gtagtggtga tgttaggcat cgtttggtat ggcggaagca
    15541 tgattttgga caacaaggga accaatggtt ttgatggacc gcaattcatc ggatttattt
    15601 tggttttttc acaactattg cgtcccattc aaggaattgc aacctctatt gccaatttaa
    15661 acaaagcaca cgcagcgcaa gatcgcatca atgaaatatt gaacgccgac gaaaaaattc
    15721 acgaagttga acatcctgtt gaaaacaaac ctttaacaac aaatgtaagc tacaacgatg
    15781 tttgtttttc gtataacgat gaacccgttc tcaaaaacgt atcgtttacc attccaaaag
    15841 ggaaaacggt ggcgttagtg ggcgaatcgg gcagtggaaa atctaccatt tctgatttat
    15901 tgcctcgttt ttacgatgta gtgagtggct caatttctat tgacaacact gatattcgtg
    15961 agttaaaaat taccgactta cgcgataaaa ttggaattgt aagccaagaa tccattcttt
    16021 tcaacggaac agttgccgaa aacattgctt ttggaaaacc caacgctacg cgccaagaaa
    16081 ttattgaagc agccaaaatt gccaatgccc ataaattcat tgaagaattg gaaaatggat
    16141 acgacacaca aattggtgaa cgcggaaata aactatcggg tggacaaaaa caacgtgtca
    16201 gcattgcccg tgccgtctta aaaaatccag aaatattaat tctcgacgaa gcaacatcag
    16261 cgctggatac cgaatctgaa aaattggtgc aagaagcgct caacaaacta atggcagaac
    16321 gaacatcgtt ggttattgct catagattga gcaccatcat ccatgccgat gaaatcattg
    16381 ttttatcgaa aggagaaatc attgaacgcg gaacacatgc cgaattattg gcaaaaggcg
    16441 gaacttatgc ctatttatcg tcattgcaag gaattgctta aataatcact cgctcaatgt
    16501 gcatatcgcc aaatgcatac ggaatataag ccaaacttgg aatttctttc gtcagaataa
    16561 ttggtgatct tcttcccaaa gcaattcgtc catgtgttgc actcaatccc attgccgccg
    16621 ctgcattgat agttagtgca tttaatgctt cttcgggagt cattttcatt ttcacacatg
    16681 ccaacgacca cacaaaaccg aggttaccac ttggagttgt tccggggtta aaatcgctcg
    16741 ccaaagcaac cggcaaatcg ttttccatca tccaacgcgc attgccaaaa ggaatggaaa
    16801 taaaatgcga acaacttggt aaaatagtag gaatggtcgt tgaattttta aggcaatcaa
    16861 tatcctcttg cgaaatttct tctaaatgat caaccgataa agcacctaat tcaactgctt
    16921 tttggatgcc tcccatgatt gaaaactgat tgacatgcac tttgggttgc aagccgtatt
    16981 ttttaccagc caaaagaatt tcttccattt gttgcaccga aaaataa
//
LOCUS       DAOQVQ010000006        14625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_4647_length_14625_cov_3.168771, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000006 DAOQVQ010000000
VERSION     DAOQVQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4647_length_14625_cov_3.168771"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2599
                     /locus_tag="PKN22_00480"
     CDS             <1..2599
                     /locus_tag="PKN22_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNS41205.1"
                     /translation="GGSTYTLTVIPRNTQFIIYDTVNISEQNYKLPIVYTRTPYDKNS
                     DDIGWQLFPFMGYAYAMQDMRGRYSSEGVYFPMYSDGWAKTNYLPLVEIPMDITVQSD
                     ANNSLKHHDGSQSIFYLADSLYRIDDVNSDGIVDTLLYCNGNIGMFGASALGNSQYQA
                     LSDIPFTENNPLKCILPVVAANEHYDSTLFHNGVYRHSLTNGWMKGQLLSGVDNSLNA
                     IDAADITNNIHSTSDYNYLDNMELANDLIDWFVATNLGFTPACQHPSSKYRTILDASK
                     APVNSQGYSDGNGTTTRYKNLNVPSYNLTGWWDIFINGQIETFNKIKHENPGVTNKIV
                     IGPWTHQTIGGTDVGDETYPDNVKDVLSIDLDIDPTTILSDSTAVNKIYRSELLKWYR
                     SNLGGEPFFIIPKSSNWQSVGTSQVRIPASNYIIPYYKFLNYLGGVSGLNNIPVEINN
                     GTGSSTINLNFPAINPPLFPLTTPLTAVDVNYFNQVSDVRMYITGPSNDAQNTNVGNY
                     WLSNDSIPFKRGIQNEVFYLHQNQTLNNQPPINNEGVLSYIADPNNPVATVGGNNMIP
                     TIPNGTKKSQGSMDMTNALYDYLTMNRSDVLQFISDPISDTLSFVGFPKAKIYAKGKT
                     STYATSKTDYDIMVRVLDVYPDGREMFITEGVVNAKAREYAKSIAQGDTNDNLILSNI
                     NNDEYYYFEFDLLPLGHTFGKNHQIKLLLSSSNFPKYQSNPHIPHEDGDFFRWEPGST
                     QTYNYYGQTLTAQNAEITYDFNPNFPSLISFPRLDTTFFTAHNNVVETLPNFDIYPNP
                     TTDKVTVVWNKSFKGDILIYDYWGKCVKTITTDNNKLMTKIDLNGLSNGFYIVRIPEL
                     NCSKKLIKN"
     gene            2746..3003
                     /gene="yidD"
                     /locus_tag="PKN22_00485"
     CDS             2746..3003
                     /gene="yidD"
                     /locus_tag="PKN22_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNS41206.1"
                     /translation="MKIISKIFSFPLIVLVRFYQMVISPLTPQSCRYTPTCSAYMLEA
                     INIWGPIKGTWLGIKRISSCHPWGGHGHDPVPTKNTSSDKK"
     gene            3016..3489
                     /gene="rlmH"
                     /locus_tag="PKN22_00490"
     CDS             3016..3489
                     /gene="rlmH"
                     /locus_tag="PKN22_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNS41207.1"
                     /translation="MKITFICIGKTSKKFLEEGEQEYLNRLKNYTKIERIELPDLKNA
                     RKLTLEQIKKEEGQLFLSKIPQGDELILLDERGKHYSSVEFSKFIDKKNINGVKGVTF
                     LVGGAYGFSDEIYARTNSKMSLSTMTFSHQMIRMIFFEQLYRAFTIIKGEPYHHE"
     gene            complement(3497..6274)
                     /gene="uvrA"
                     /locus_tag="PKN22_00495"
     CDS             complement(3497..6274)
                     /gene="uvrA"
                     /locus_tag="PKN22_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNS41208.1"
                     /translation="MAKQSFIEIKGARVNNLKNIDVQIPHGKFTVITGLSGSGKSSLA
                     FDTLYAEGQRRYIESLSSYARQFLGKLDKPETDYIKGIAPAIAIEQKVISNNPRSTVG
                     TTTEIYDYLKLLFARAGKTYSPISGKEVKRHTVTDVIQFIESLPNTERLLILAPIAAI
                     RQYGAQQAVKILIQQGYTRLLVDQEIVNLDAVEPSKITNDNALLVVDRATADTSSEEL
                     LGRLEDSVHLAFTEGNGECFVFTMSDKQMHAFSTRFELDGIEFQIPNDHLFAFNNPIG
                     ACKSCEGYGQIIGIDEDLVIPNKNLSVYEGALLPWKGEMMKEYLNELIINSSKFDFPI
                     HRPLKDLTAEQYQLLWKGNRYFTGLNEFFKFLETQTYKIHYRVLLSRYRGKTQCPECR
                     GTRLRSDANNVKIEGKSITDIVLSPIEEILPFFKNLKLEVHQQQISKRVLPEIVNRLE
                     FLTNVGLGYLTLNRQSNTLSGDESQRINLATSLGSSLVGSMYILDEPSIGLHPRDTER
                     LILVLNKLRDLGNTVIVVEHEEDIMRAADEILDIGPMAGVYGGEVVFQGDFKQLVKAK
                     NSITADYLTGKKAIAVPQKRRKQINFLHFSGVRKHNLKDVEVKIPLHNFVSITGVSGS
                     GKSTLIKDIVYPAVRNNLGEIALATKNFEVNGDWKHIGAVELVDQNPIGKSSRSNPVT
                     YVKAFDDIRELYAKQQISKIRNYRPGYFSFNVEGGRCEMCEGEGIITVGMQFMADIHL
                     TCETCHGTRYKAETLEVEYRGVNIAQLLNMDIEKALEFFNEGKDLLEKKIAEKLQPLV
                     DVGLGYLKMGQSSSTLSGGEAQRIKLASFLAKGSKATPTLFIFDEPTTGLHFHDIEKL
                     IIALNRLIDAGHSVIVIEHNKEVIKCADWVIDLGPEGGDKGGNIVFQGTPEDLVQCSE
                     SYTGKFLKDVL"
     gene            complement(6343..7215)
                     /locus_tag="PKN22_00500"
     CDS             complement(6343..7215)
                     /locus_tag="PKN22_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNS41209.1"
                     /translation="MNNTTNIEALIQLMDDPDEGIFLHVRDRLLQYGPDAIPYLENSW
                     GEKDYGLIFLNRVEQLIHDIQFEDVKKQLQQWQNSEKDLLKGAIIIAKYQYPGLHEEH
                     IFEFIEHVRKDAWLELSNRHTAYEKVRILNKIFFDLHHFKGDSKNFHSPLNSFINTVI
                     EQRKGNPLSLTILYSIIAQSLNMPIYGVNLPNHFIAAYIDEDGINQFVNKENNNGVLF
                     YINVFSRGSLFDEEEINEFLKKLNIAPTREYFEPCSNTTILRRMLTNLITSFQQAGNA
                     EKVNELIQLRDLLA"
     gene            complement(7369..8634)
                     /locus_tag="PKN22_00505"
     CDS             complement(7369..8634)
                     /locus_tag="PKN22_00505"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HNS41210.1"
                     /translation="MAEVVYMPKLSDTMTEGVVSEWTKKVGDKVASGEVLAEIETDKA
                     TMEFESFYDGVLLHIGVQKGEAAPVNALLAIIGKEGEDVSAILANAGTAPAPKTEAKV
                     EVTPSPEPVKAVAPEVPKVESKPAPVVAAPVAHDGRVVASPLAKKMAEEKGIDINSVL
                     GTGDGGRIVKRDIDHYVPYEAPVRAHATATAAVGVESYTDENISQMRKTIARRLAESK
                     FTAPHFYLTLEIDMDNAIAARKALNANEGVKISFNDLVLKAVAVALRQHPTVNSSWLG
                     DKIRRNHHVHIGVAVAVDEGLLVPVVRFADTKGLVQIGEEVKEYAKKAKDKKLQPSDW
                     EGNTFTISNLGMFGIDTFTAIVNPPDSCILAVGGIKQVPVVKNGQVVPGNIMKVTLSC
                     DHRVVDGASGAAFLQTVKGYLENPVTLLL"
     gene            complement(8650..9702)
                     /gene="pdhA"
                     /locus_tag="PKN22_00510"
     CDS             complement(8650..9702)
                     /gene="pdhA"
                     /locus_tag="PKN22_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNS41211.1"
                     /translation="MAAKTSTKPTSTTKKSKVENTSKFSKETYIKWWKDMLLIRKFEE
                     KAGQLYIQQKFGGFLHLYIGQEAIVAGTASACKPGDKHMTAYRDHGHPIALGTDPRVL
                     MAELYGKATGCSKGKGGSMHFFDKEKNFMGGHGIVGAQIGMGAGVAFAEKYNKTENVV
                     FVSMGDGAARQGILHETFNMAMNWKLPVIFIIENNNYAMGTSVQRTTNVHDLSKMALS
                     YEMPSIVVNGMKPESVHDAIEEAATRARKGGGPTLLDIRTYRYKGHSMSDPQKYRTKE
                     EVNEWIEKDPITHCLDVIKDNKWLSDKEIEEIENWVKNEVDESVKFAEESPLPTADEL
                     YKDIYAEPNYPYIVEY"
     gene            complement(9865..10356)
                     /locus_tag="PKN22_00515"
     CDS             complement(9865..10356)
                     /locus_tag="PKN22_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41212.1"
                     /translation="MKNTATKTFLFTLLVVAFTLFSGCKKACKSLGENVTSGEIKTDV
                     AVYPESGYMTSSMTQSQYLITGSSNYANRFKISFDNGETKQSVNYGQYSILCYPVTTS
                     CFAQFDRNVKIDDANGIVKYTIKVKECGKCTSERTTENYVLIRAVPDTYQVVYDVDIE
                     HID"
     gene            complement(10423..11151)
                     /gene="lptB"
                     /locus_tag="PKN22_00520"
     CDS             complement(10423..11151)
                     /gene="lptB"
                     /locus_tag="PKN22_00520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNS41213.1"
                     /translation="MKLYTENIKKVYKGRNVVKGVSVEVNQGEIVGLLGPNGAGKTTS
                     FYMIVGLVSPDEGKVYIDGTEITNMPMYKRSQLGIGYLPQEISVFRKLSVEDNIMAIL
                     EMTDMTKVQREERLEQLLSEFSLNHVRKNLGNRLSGGEKRRTEIARALATNPKFLLLD
                     EPFAGVDPIAVEDIQSIVSELRQRNIGILITDHNVQETLSITDRAYLLFEGAILKQGT
                     AEELAADEQVRKVYLGQKFELRKK"
     gene            complement(11162..13621)
                     /locus_tag="PKN22_00525"
     CDS             complement(11162..13621)
                     /locus_tag="PKN22_00525"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNS41214.1"
                     /translation="MTKLFLILTFLLPLALYSQETIVKGTVIDGTTGETLPFVKVQFV
                     DSKIGTITDSVGHYELKSYYATDSVRFSLPGYIPQTYKIKRDKEQLFEIVLTVAVAEI
                     QDVTILPPDELPSVTLHKKIVKNKPINDRAKLKAYEYELYNKMQVDLNNIGDKFKDRG
                     IVKKMDLILNYLDSADNGKKYLPVILTEGLSDYYYTTNPVRKREVLKATKVSGVKNLQ
                     FDQYLGEMYMDINVYDNNITIVNRSFISPISNIARNYYQFLITDSSFIDNQWCYKMSF
                     RPKRTGDLTFEGEMWVHDTTYAIKSIKGTIAADANINYIQDFYFEQNFDMVEKEVWML
                     TDETMFANINVTQKSALYGVFVRKYSSRKKFVINKPHPDKFYNSNSTVEMADSAKLRD
                     EAYWVAHRHVPLTSSQEGITEMLDSLNTLPFYKNLQGITRLLPTGYYPYGWFEFGHLY
                     KLISFNPVEKFRTGFAIRTSNNFSKVVELGGKLYYGFGDKKFKYGGSVRFNTSKKKRG
                     LLSLFYEYDIEQIGASPKASQVGSTFGTLFRTGPLDKLTFVQKTGINFEKDIHKDFIL
                     FSGVELKEYKALGKANYVRFNETNGLYDTINKIRTFEVTMRLRWSKDEEFIGGVYDRV
                     AIKSKYPVLSFQTILGIKGAIESDYKYQKYEFTYDHRVMAGVIGYIRYGFGLGYIHGT
                     TAYPFLKVHEGNQSFYLNKSTFNKLNFFEFISDKYVDGYIENHWGGLFLDYIPWINKL
                     KMRFVTTARATWGTISPRHTKEMLLPSFTKQFGKIPYLEVSLGLENILNLIRIDVFYR
                     VTHQIPGASPFGVRARIEMFL"
     gene            complement(13700..14485)
                     /gene="tatC"
                     /locus_tag="PKN22_00530"
     CDS             complement(13700..14485)
                     /gene="tatC"
                     /locus_tag="PKN22_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNS41215.1"
                     /translation="MSEEKNMPFLQHLEELRWRLVRAAIAVLICSILLFWQQTWIMDN
                     IFLVMLDNNFISFRLMCEWFGVCTESAPVIIQSMNVSGQFSYAMLTSILGGVVIASPF
                     IFYQLWMFIKPGLKEKERKASSGLIFYISLLFILGILFGYFIITPLAIQFFGSYQISS
                     DIQNNFTIDSYMGMILSTTFYTGLFFLLPVVSYLMTSLGVISSDFLKKYRKHAIIVVL
                     ILSAVITPPDVISQIIVAIPITILYEIGIFASKRVERRKNKTL"
BASE COUNT         4588 a         2779 c         2452 g         4806 t
ORIGIN      
        1 tggaggttca acatatacat taacggtaat tccaagaaac actcaattta ttatttacga
       61 tacagtaaat atttctgagc aaaactacaa actccctatt gtttacacgc gcacacctta
      121 tgacaaaaac tccgatgata taggttggca attatttccc tttatgggat atgcttatgc
      181 aatgcaagat atgagagggc gatatagcag tgaaggggtt tattttccga tgtattcaga
      241 tggatgggct aaaacaaatt atctaccact tgttgaaatt ccaatggata ttactgtaca
      301 atcagatgca aataattcac ttaaacacca tgatggaagc caatccattt tttatttagc
      361 tgatagtttg tatcgcattg atgatgtaaa ttctgacggg atagttgata cactattgta
      421 ttgcaatggg aacattggga tgtttggagc atcggcctta ggaaattctc aataccaagc
      481 gttgtcagac atacctttta cagaaaataa tccattaaaa tgcatattgc ccgtagttgc
      541 agcaaatgaa cattatgatt caacgttatt tcataatggg gtttatcgcc attcgctaac
      601 aaatggttgg atgaaaggtc aactattatc tggagttgac aactctttga acgccattga
      661 tgcagcagat attaccaata acattcacag cacttctgac tataattatt tagataacat
      721 ggaattggct aatgatttaa ttgattggtt tgttgcaaca aatttgggtt ttacaccagc
      781 atgccaacat ccatcttcta aatatagaac aatattagat gcgtcaaaag cccccgtaaa
      841 ttctcaagga tattctgacg gcaatggcac aactacacgc tacaaaaacc taaacgttcc
      901 ttcgtataat ttaactggat ggtgggatat tttcataaat ggtcaaatag agacttttaa
      961 taaaataaaa catgaaaacc ctggcgttac taataaaatt gtaattggcc cttggacgca
     1021 tcaaacaata ggtggaactg atgttgggga tgaaacctat cctgataatg ttaaagatgt
     1081 gctttcaata gatttagaca ttgatccaac aactattctt tcggattcaa ctgcggtgaa
     1141 taaaatatat cgttctgaat tgcttaaatg gtaccgttcc aatttaggtg gagaaccatt
     1201 ttttattatc ccaaaatctt ctaattggca aagtgtagga acatcgcagg tgagaattcc
     1261 ggcgtcaaat tacattattc cttactataa atttttaaat tatttgggtg gagtatcagg
     1321 tttaaacaat attcctgttg aaattaacaa tggtacaggg agctcaacca tcaatttaaa
     1381 ctttccagct ataaatccac cgttatttcc acttacaact cctttaacag ctgttgatgt
     1441 aaattatttt aatcaagtga gcgatgtgag aatgtacatt acaggccctt ctaacgatgc
     1501 tcaaaatacc aatgtgggca actattggct ttctaatgat tctattccat tcaaaagagg
     1561 tattcaaaat gaagtttttt acttgcacca aaatcaaacc ctaaataatc aaccaccaat
     1621 caataatgaa ggtgtgttgt cttatattgc cgaccccaat aatcctgtag caacagtggg
     1681 tggaaacaat atgattccaa ctattccaaa tggaacaaaa aaatcacaag gttctatgga
     1741 tatgaccaat gctttgtatg attatttaac aatgaatagg agtgatgtgc ttcaatttat
     1801 ttcagatcca attagtgata cgttgtcttt tgttggtttt ccaaaagcta aaatttatgc
     1861 caaaggaaaa acatcaactt atgcaacttc taagacagat tatgacataa tggtaagagt
     1921 gttagatgtt tatcctgatg gtagagaaat gtttatcaca gaaggggttg ttaacgctaa
     1981 agcacgtgaa tatgctaagt cgattgcaca aggagatacc aacgacaatt tgattttgtc
     2041 gaacatcaac aacgatgaat attattattt tgaatttgat ttattgcctt tgggacatac
     2101 atttggaaaa aaccaccaaa taaaattgtt acttagcagc tctaattttc caaaatacca
     2161 aagcaatccg cacattccac acgaagatgg tgactttttc agatgggaac ctggaagcac
     2221 acaaacgtat aattattacg gacaaacgtt aactgctcaa aatgctgaaa ttacgtatga
     2281 tttcaaccct aattttccaa gtttaatttc ttttcctcgt ttggatacaa cattttttac
     2341 tgcacataac aatgtagttg aaacactacc aaattttgat atttatccca acccaacaac
     2401 tgataaagtc acagttgtat ggaataaatc gttcaaaggc gatattttaa tttacgatta
     2461 ttgggggaaa tgtgtgaaaa ctataacgac cgacaacaat aaactaatga ctaaaattga
     2521 tttgaatgga ttgtccaatg gattttacat tgtaagaatt ccagaattaa actgctcaaa
     2581 gaaattaatt aaaaactaac attaattatt taagtgttta atttatattt tgttgaatta
     2641 cagataaata aatagcattg attaaagata ttgtataaca aaatagatta cttataatct
     2701 gttgttagta gttatttgtt tgtgagcttt tattaccttt gcaccatgaa aatcatttcc
     2761 aaaattttca gttttccact aattgtattg gtgcgctttt accaaatggt tattagtccg
     2821 ctgacacctc aaagttgccg ctatacaccc acgtgctctg catacatgct ggaagctatt
     2881 aatatttggg gacctatcaa aggaacgtgg ttgggaataa aacgcatatc ttcttgccat
     2941 ccctggggcg gacatggaca tgatcctgtt ccaacaaaaa atacatcttc tgacaaaaaa
     3001 taatctccat tttacatgaa aattacattt atctgcatcg gaaaaacaag caaaaaattt
     3061 ttagaagaag gcgaacaaga atacttgaac cgcttgaaaa attatactaa aattgagcgt
     3121 attgagttgc ccgacctaaa aaatgcacgt aaactaacat tggagcagat taaaaaagaa
     3181 gaaggacagt tgtttttatc caaaattccg caaggcgatg aattgatact attggacgaa
     3241 cgcggaaaac attattcttc ggtagaattt tcaaaattta ttgataaaaa gaacatcaac
     3301 ggagttaagg gagtaacatt tttagtaggt ggagcttatg gcttttccga cgaaatttat
     3361 gcacgaacta acagtaaaat gagtttgtca accatgacgt tttcgcacca aatgatacgc
     3421 atgatttttt ttgagcaatt gtaccgtgct tttacaatca tcaaaggcga accttatcac
     3481 cacgaatagc aacgatttat agcacgtctt tcaagaattt ccctgtatag ctttcagagc
     3541 attgcaccaa atcttcgggt gtaccttgaa atacgatatt tccaccttta tcgccccctt
     3601 ctggacctaa atcaatcacc caatcggcac atttaatcac ttctttgttg tgttcaatta
     3661 caattaccga atgtccagca tctatcaaac ggttgagtgc aattattaac ttttcaatat
     3721 cgtgaaaatg caagccagtt gtgggttcat caaaaataaa aagggtagga gtggccttac
     3781 ttcctttagc taaaaacgaa gctaatttga tgcgctgcgc ctcgccgcca ctcaatgtgc
     3841 tagacgattg tcccattttc aaatacccca aaccaacatc caccaaaggt tgtaattttt
     3901 cggcaatttt cttttccaat aaatccttgc cttcattgaa aaactccaat gccttttcaa
     3961 tgtccatgtt caatagctga gcaatgttca ctcctctgta ttccacctcc aaagtttcgg
     4021 ctttgtaacg cgttccgtgg catgtttcgc acgttaaatg aatgtcggcc ataaactgca
     4081 ttccaacagt aataattcct tcgccttcgc acatttcaca gcgaccgcct tccacgttaa
     4141 aactaaaata ccccggacgg tagtttctaa tttttgaaat ttgttgtttg gcatacaact
     4201 cacgaatgtc gtcaaaagct ttaacgtagg ttacaggatt acttcgcgaa gatttaccaa
     4261 taggattttg atccactaac tccacagcgc caatatgttt ccaatcgcca ttcacctcaa
     4321 aatttttggt tgccaaagca atttctccca aattattgcg cactgccgga taaacaatgt
     4381 ctttgattaa ggttgatttt ccagaaccac tcacaccggt aatactcaca aaattgtgca
     4441 acggaatttt cacttctaca tctttcaaat tgtgttttcg aacacctgaa aaatgcaaga
     4501 aattgatttg ttttctacgt ttttgaggta cagcaatggc ttttttacct gtcaaataat
     4561 cagcagtgat gctatttttt gcttttacca attgtttgaa atcgccttgg aataccactt
     4621 caccgccata tacacccgcc atcggaccaa tgtctaaaat ttcatcggct gcacgcatga
     4681 tgtcttcttc gtgctctacc acaattacgg tgttgcccaa atcacgcaat ttattcaaaa
     4741 ctagaatcaa tcgttccgta tcgcgtgggt gcaagccaat ggagggctcg tctaaaatat
     4801 acatcgaccc aactaagcta ctacccaatg aagttgccaa gttaatgcgt tgcgattcat
     4861 ctccagaaag ggtgtttgat tgtcggttga gtgttaaata gcccaagcct acattggtta
     4921 aaaactccaa acggtttaca atttcgggca atacacgttt tgaaatttgt tgctgatgaa
     4981 cttctaattt taaatttttg aagaacggca aaatttcttc aataggtgac aaaacaatat
     5041 cggtgatgga ttttccttca atttttacgt tgttggcatc gcttctcaat cgggttcctc
     5101 tacattctgg acattgtgtt ttgccacggt agcgcgacaa cagaacacgg taatgaattt
     5161 tataggtttg cgtttccaaa aacttgaaaa actcattgag tccggtaaaa taacggtttc
     5221 ccttccacaa caattggtat tgctcggctg ttaaatcttt taatggtcga tgaatcggaa
     5281 aatcaaattt agatgaattg ataatgagct cgttcaaata ttccttcatc atttcgcctt
     5341 tccacggaag taaagcccct tcgtaaacac tcaaattttt gttcggaatc accaaatctt
     5401 catcaatacc aatgatttga ccgtatcctt cgcacgattt acacgcacca attggattat
     5461 tgaaagcaaa taaatgatcg ttgggaattt gaaattcaat gccgtctaac tcaaatcggg
     5521 tagagaaagc gtgcatttgc ttgtcagaca tcgtaaaaac aaagcattca ccatttcctt
     5581 ctgtaaaagc taggtgaaca gaatcttcca aacgccctag caattcttcg gatgacgtat
     5641 cggcagttgc tcggtccacc accaaaagtg cattatcgtt ggttattttc gacggttcaa
     5701 ctgcatccaa gttaacaatt tcttgatcca ccaacaaacg ggtatatcct tgctgtatca
     5761 atatttttac tgcttgttga gcgccatatt gacgaattgc agcaatgggt gctagtatca
     5821 acaaacgttc ggtattgggc aatgattcaa taaattgaat aacatctgtc accgtatgcc
     5881 gttttacttc tttaccacta attggcgaat aggtttttcc agcacgtgca aataaaagtt
     5941 tgaggtaatc gtatatttcg gtagttgttc caacggttga tcgcggattg ttggaaatga
     6001 ccttttgctc tatggctatg gcaggagcaa ttcctttgat atagtcggtt tctggtttat
     6061 cgagcttgcc taaaaattgg cgggcatacg acgataaact ttcgatgtaa cgacgttgtc
     6121 cctcggcata taaggtatca aaagccaacg atgattttcc agagcccgaa agtcctgtaa
     6181 ttacagtaaa cttgccatgt ggaatttgta catcgatgtt tttaaggttg ttcacccttg
     6241 cacctttaat ctcaataaac gactgttttg ccatgttaca aaattaatgg ttgttttgct
     6301 gaaatactaa attaattaag aataatgcta aaaatagatg gtttatgcca ataaatcgcg
     6361 caactgaatg agttcgttaa ctttttcagc atttccagcc tgttgaaacg aggtaatgag
     6421 attggttagc atgcggcgta aaatggtggt gttggagcaa ggctcaaaat attctcttgt
     6481 tggagcaatg ttgagtttct ttaaaaattc gttgatttct tcctcgtcaa acaaactgcc
     6541 tctggaaaaa acattgatgt aaaacaaaac tccgttgtta ttttctttat tcacaaattg
     6601 attgatgccg tcttcatcaa tataagcggc tataaaatga ttaggcaaat taacaccata
     6661 aataggcata ttgagcgatt gtgcaataat ggaataaaga atagttaaac tcaatggatt
     6721 gccttttcgt tgttcaatca ctgtattgat aaacgaattg agtggtgaat gaaagttttt
     6781 agaatcgcct ttaaaatggt gtaaatcaaa gaagatctta tttaaaattc gcactttctc
     6841 ataagctgtg tggcgattac tcaattccaa ccaagcgtct tttctcacat gttcaatgaa
     6901 ctcaaaaatg tgctcttcgt gcaagcccgg atattggtat ttggcaataa taatggcccc
     6961 tttcaacaag tctttctcac tattttgcca ctgttgcaac tgttttttta catcttcaaa
     7021 ttgaatatcg tgaatgagtt gctctacacg attgagaaat atcaagccat aatctttttc
     7081 accccacgaa ttttcaaggt agggaatcgc atcaggacca tattgcaaaa gtcgatctct
     7141 tacatgtaag aaaatccctt catctggatc gtccatcagc tgaataagtg cctcaatatt
     7201 tgttgtatta ttcacactac aaatttagaa ggagaactta ttgttttttt aggacatttt
     7261 tttctttatt aaatgcaagt tgttaatttc acttaaaatc aagtgctaac aatgaaaaaa
     7321 ggactgtcac aaaacagtcc tttctctatt tttattaact tttttctatt acaacaacaa
     7381 agtaacaggg ttttccaaat aacctttaac tgtttgcaag aatgcagcac cagatgcgcc
     7441 atccacaaca cggtggtcgc aagaaagagt tactttcatg atgtttcctg gcaccacttg
     7501 tccatttttc acaacaggaa cttgtttaat tcctccaaca gctaaaatac agctatctgg
     7561 tgggtttaca attgctgtaa atgtatcaat tccaaacatt ccaagattag aaatggtaaa
     7621 tgtgttacct tcccaatcgg atggttgtaa ttttttatct tttgcttttt tagcgtattc
     7681 tttaacttct tcgccaattt gcactaatcc ttttgtatct gcaaaacgaa caacaggaac
     7741 caataatcct tcgtccacag caacagctac tccaatgtgc acgtggtgat ttctacgaat
     7801 cttatcaccc aaccaagaag agttaaccgt aggatgttga cgcaatgcaa cggcaactgc
     7861 tttcaaaact aaatcattga aagaaatttt aacaccttca ttagcattta atgcttttcg
     7921 agctgcaatg gcattgtcca tatcaatttc caatgttaaa tagaaatgcg gagcggtgaa
     7981 tttgctttca gccaatcggc gagcaattgt tttgcgcatt tgagaaatat tctcatctgt
     8041 ataagattcc acaccaacag cagctgtagc agttgcgtgt gctctaactg gtgcttcgta
     8101 aggaacataa tgatcgatgt ctctttttac aattcttcca ccatcgcccg ttccaagtac
     8161 gctgttgata tcaattcctt tttcttcggc cattttttta gccaatggag aagcaactac
     8221 gcgaccatcg tgtgcaacag gcgcagcaac tactggagct ggtttacttt caacttttgg
     8281 aacttctgga gcaaccgctt ttactggctc tggagaagga gttacttcta cttttgcttc
     8341 agtctttgga gcaggtgcag ttccagcgtt tgccaaaata gcactcacat cttcaccttc
     8401 tttaccaata attgccaaca aagcatttac tggtgcagct tcaccttttt gaacaccaat
     8461 gtgcaataaa acgccatcgt aaaaagattc aaactccata gtagctttat ctgtttcaat
     8521 ttctgctaaa acttcaccag atgcaacttt atcaccaact ttttttgtcc actcagaaac
     8581 aacaccttct gtcatggtgt cgcttagttt tggcatgtaa actacttcag ccattttatt
     8641 taattttttt taatattcaa caatataagg ataatttggt tcagcataga tgtctttgta
     8701 taattcatct gctgttggaa gtggcgattc ttctgcaaat ttcacacttt catccacttc
     8761 attttttacc caattttcaa tctcttcaat ttctttgtca gacaaccatt tattgtcttt
     8821 aattacatcc aaacaatggg tgatagggtc tttttctatc cattcattaa cctcttcttt
     8881 tgtacggtat ttttgaggat ctgacatgga gtgaccttta taacgataag tacggatatc
     8941 caatagagta ggaccaccac cttttcttgc tcttgttgct gcttcttcaa ttgcatcgtg
     9001 cactgattct ggcttcatac cattcaccac aatagaaggc atttcataag aaagtgccat
     9061 ttttgacaag tcatgtacgt ttgttgtacg ttgaaccgaa gttcccattg cataattgtt
     9121 gttttcgatg ataaaaatca ctggtagctt ccaattcatt gccatgttaa acgtttcatg
     9181 caaaatacct tgtcttgctg caccgtcacc cattgaaaca aaaacaacat tttctgtttt
     9241 gttatatttt tccgcaaaag caacacctgc acccatacca atttgagcac ccacaattcc
     9301 gtgaccaccc atgaagtttt tctctttatc aaaaaagtgc attgaaccac cttttccttt
     9361 agaacaacca gttgcttttc catacaattc ggccatcaac acacgtgggt ctgttcctag
     9421 cgcaattgga tgtccgtgat cacgataggc tgtcatgtgt ttatcgcccg gtttacaagc
     9481 gcttgctgtt cctgcaacta ttgcttcttg accaatgtat aagtgtaaga atccaccaaa
     9541 tttttgttga atgtacagtt gacctgcttt ctcttcaaat tttcggatta aaagcatgtc
     9601 tttccaccat ttgatatacg tttctttcga aaacttagat gtgttttcta ctttactttt
     9661 ttttgttgta gatgttggtt ttgtggaggt ttttgccgcc ataattttca aattttaata
     9721 caagaagcaa atatatgtaa aaaaatacga tagttgaaag agaaataaaa ttaattaaag
     9781 attaaaaaaa acgattttta aaaaacattt gaatataaaa aaagccccat tcaattggag
     9841 ctttcgtttt aaaattcaat attattagtc aatgtgttca atatccacat cataaaccac
     9901 ttgataagta tctggaacgg cgcgaattaa tacgtaattt tcggttgtgc gctcactcgt
     9961 acattttcca cattctttca ctttgatagt gtatttaaca atgccatttg catcgtcaat
    10021 ttttacattt ctatcaaatt gtgcaaagca acttgttgta acagggtaac acaaaatact
    10081 gtattgaccg tagttgacag attgttttgt ttctccatta tcaaaactga ttttaaatct
    10141 gtttgcataa ttgctacttc ccgtaattaa atactgtgat tgcgtcatag agcttgtcat
    10201 gtagccagat tcagggtaaa cagcaacatc agttttaatt tcaccagaag ttacgttttc
    10261 acctaagctt ttacatgctt ttttacatcc agaaaataga gtaaatgcta ccactaatag
    10321 tgtaaaaaga aatgtttttg tagctgtatt tttcataaat atgggttaaa ttcagttatt
    10381 atttgatttc aaagatataa aaaatcttta atgacgataa aattattttt ttcttaattc
    10441 aaatttttgt cctaagtaca cttttctaac ttgttcatct gctgctaatt cttcggctgt
    10501 gccttgtttt aaaatagcac cttcaaacaa taaataggct ctatcggtaa tggataaagt
    10561 ttcttgcaca ttgtggtcgg taattaaaat accgatgttg cgctgacgta gttcagaaac
    10621 gatactttga atatcttcaa cggcaatggg gtcaacacca gcaaatggtt cgtcgagcaa
    10681 taaaaatttt gggttggttg ccaaagcacg tgcaatttcg gttcttcgtt tttctcctcc
    10741 cgaaagtcga ttccctaaat ttttacgaac gtgatttaaa ctaaattcag aaagaagttg
    10801 ctccaagcgt tcttctcgct gcactttggt catatccgtc atttccaaaa tagccatgat
    10861 gttatcttcc acactcagtt ttctaaaaac agaaatttct tgtggcaaat atccaattcc
    10921 taattgtgaa cgtttataca tcggcatgtt ggttatctcc gttccgtcaa tgtaaacctt
    10981 tccttcatca gggctaacta agccaactat catatagaat gatgttgttt tacctgcacc
    11041 atttgggcct aacaatccaa cgatttcacc ttgatttact tcaactgata caccttttac
    11101 aacgtttcta cctttgtaaa cttttttaat attttcggta tataacttca ttctaatttc
    11161 attataagaa catttcaatt ctagctctca ctccaaaagg agaagcgcct ggaatttggt
    11221 gtgtgactct ataaaatacg tcaattcgaa tcaaatttag aatattttcc aaacctaacg
    11281 atacttctag gtaaggaatc tttccaaatt gtttggtaaa tgaaggtagg agcatttctt
    11341 tggtgtggcg cggacttatt gtgccccaag tagcacgtgc agtggttaca aatctcattt
    11401 ttaatttatt aatccatgga atgtagtcta aaaacaaacc tccccaatgg ttttcaatgt
    11461 aaccatccac gtatttgtcg gatataaatt caaagaaatt taatttgtta aaagtggatt
    11521 tattcaaata aaaagattga tttccttcgt gaacttttaa gaatggataa gctgttgtgc
    11581 cgtgaatata gcccaatcca aatccatagc gaatgtaacc tataacacct gccattactc
    11641 gatggtcata agtgaattcg tatttttggt atttataatc gctttcaata gctcctttta
    11701 ttcctaaaat tgtttgaaag gacaatacgg gatatttgga ttttattgcc actctgtcat
    11761 aaacacctcc aataaattct tcgtcttttg accaacgtaa acgcatcgta acttcaaatg
    11821 ttctgatttt attgatggta tcatacaagc catttgtttc gttaaaacgc acatagttgg
    11881 ccttgcccaa tgctttgtat tctttgagtt caacgccaga aaacaagata aaatctttgt
    11941 gaatgtcttt ttcaaagttg atgcctgttt tttgaacaaa agttaattta tccaacggtc
    12001 cagttctaaa caatgttcca aaggttgaac caacttgcga tgcttttgga gaagcaccaa
    12061 tttgttcgat gtcgtattca taaaataagc tcaataaacc tcgttttttc ttggaagtat
    12121 taaaacgcac actcccccca tatttgaatt ttttatcgcc aaaaccgtaa tacaatttac
    12181 cgcccaattc aacaactttt gaaaaattat tagaggtgcg aatggcaaaa ccagttctaa
    12241 atttttctac tggattaaaa ctaatcaatt tgtagagatg accaaattca aaccacccat
    12301 aaggataata tcctgttggc aataggcgag tgatgccttg taaattttta taaaacggca
    12361 acgtatttaa tgaatccaac atttctgtaa ttccttcttg tgatgacgtg agtggaacat
    12421 ggcgatgggc aacccaatag gcttcgtctc tcaattttgc gctatctgcc atttcaaccg
    12481 tactattact gttgtaaaac ttatcagggt gaggcttgtt gatgacgaat ttctttctgg
    12541 atgaatactt gcgtacaaaa acaccataca aagcagattt ttgcgttacg ttgatgtttg
    12601 caaacatggt ttcatcggtc aacatccaca cttctttctc caccatgtca aaattttgct
    12661 caaaataaaa atcttgaatg tagttgatgt tggcatctgc tgcaattgtt cctttgatag
    12721 attttatggc ataggtggta tcgtgcaccc acatttcacc ttcaaacgtt aaatcgccag
    12781 ttcgttttgg acgaaaactc attttataac accattgatt gtcgataaaa gatgaatcgg
    12841 tgattaaaaa ttggtaataa tttctagcaa tgttggaaat tggactgata aaacttcggt
    12901 taacgatagt gatattgttg tcataaacat tgatgtccat gtacatttcg cccaaatatt
    12961 gatcaaattg aaggtttttg actccagaaa ctttggtagc ctttaaaact tctctttttc
    13021 ggacaggatt ggtagtgtag taataatcgg ataatccttc ggttaaaatt acaggcaaat
    13081 atttttttcc attatcggca gaatcaaggt aattgagaat taaatccatc ttttttacga
    13141 tgcctcggtc tttaaattta tcgccaatat tgttcaaatc aacctgcatt ttgttgtaca
    13201 attcgtattc atacgctttc aacttagcac ggtcgttaat gggtttgttt tttactattt
    13261 ttttgtgtag tgtaaccgat ggtaattcat ctggaggtag aatcgtaaca tcttgaattt
    13321 ctgcaacagc aacggttaaa acaatctcaa agagctgttc tttatcacgt ttaattttat
    13381 aggtttgtgg aatgtatcct ggcagtgaaa accgaacaga atcggtggcg taataggatt
    13441 ttaattcata atgcccaaca gaatcggtaa tggttccaat tttagaatca acaaattgaa
    13501 cttttacaaa gggtaaggtt tcacctgtag ttccgtcaat tacagttcct ttcacaatgg
    13561 tctcttgcga atagagcgcc agtggtaaaa gaaaagttaa tatcaggaat aatttagtca
    13621 ttaatttgtt ttatataaag tagtttcaaa aaattgtttg cctgagttca aaaaaacgaa
    13681 tattcatacc tttaaacgat taaagagttt tgttttttct tcgctcaacg cgttttgaag
    13741 caaatattcc tatttcatac aaaatggtaa taggaatagc cacaatgatt tggctaatta
    13801 catctggagg agttatcaca gcagaaagaa taagcacgac aattatcgca tgttttctgt
    13861 atttttttaa aaaatcgctg gaaataacac ctaaactagt cattaaataa gaaacaacag
    13921 gtaatagaaa aaacaaacca gtataaaatg tggttgataa aatcatcccc atataggaat
    13981 cgattgtaaa attattttga atatcggaag atatttgata acttccaaaa aactgaatgg
    14041 ctaatggcgt aataataaag tagccgaata aaattccgag aatgaacaaa agggaaatat
    14101 aaaatatcaa gccacttgat gcttttcgtt ctttttcttt taagcctggc ttgataaaca
    14161 tccacaattg ataaaaaata aacggtgagg caataacaac tcctcccaag atactggtta
    14221 acattgcgta ggaaaattga cccgaaacat tcatgctttg gataatcaca ggagcagatt
    14281 cagtacatac accaaaccat tcacacataa ggcgaaagga aatgaagtta ttgtccaaca
    14341 ttacaaggaa aatattgtcc attatccaag tttgttgcca aaataatagt atagaacata
    14401 tcaatacagc aatagctgct ctcactaatc tccatcgtaa ttcctccaaa tgttgaagga
    14461 agggcatgtt tttttcttca ctcatttgga tacaaaatta aaaaactagt taacaagtgg
    14521 gtatttattt taaaataaag ttctttttac tgtacaaaaa aagccatcta atttagatgg
    14581 cttttttaca atcattaaaa gattatttaa aattttgaag cgagt
//
LOCUS       DAOQVQ010000007        14511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_4696_length_14511_cov_3.876591, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000007 DAOQVQ010000000
VERSION     DAOQVQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 14511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14511
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4696_length_14511_cov_3.876591"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKN22_00535"
     CDS             <1..736
                     /locus_tag="PKN22_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNS41216.1"
                     /translation="LNRPMKGTVICIPIFNIFGFLNLKRELPDGRDLNRSFPGTPNGS
                     LASQFAFQFMKEIAPVVDVIIDFHTGAAQRNNHPQIRCDFNDAESLELAKVFNSPIIL
                     NSGLISKTIREVIQKLGKQYLIFEGGKANSIEELVVDEGINGAKRILSHFGMRSFKID
                     ISLGREPMFLTESKWMRASNSGLFLPHVKNGTRVKKGEIMGIIMDPFGKIERNIKAQK
                     DAVVLCVNEAPTVYKGDAIFHIAIEA"
     gene            complement(787..1296)
                     /locus_tag="PKN22_00540"
     CDS             complement(787..1296)
                     /locus_tag="PKN22_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNS41217.1"
                     /translation="MKKILVGLLMMVSFGAMAQVKIGFVDSQKLLDTMPSRKAALEKY
                     KAHEKTLADEFNLLRADLEKSYAEYQAKQGDLTPVLRQAAEKKIMDKERSVQERQETI
                     QQELQAYGEELNIPILDKVQKAIAIIAERQKLTMVVDKSTTLYHDKTMDITSVVAVEL
                     LKLENEAKK"
     gene            complement(1315..1827)
                     /locus_tag="PKN22_00545"
     CDS             complement(1315..1827)
                     /locus_tag="PKN22_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNS41218.1"
                     /translation="MKTVLYILAFVFGTGFASAQKYAYIDSDFILEKIPEYTDAKEQL
                     NKLADRWTKEIEERYAAIKTKKDNFAREEVLLPSEEKANRLKEIETMESEAMQMQRQR
                     FGVEGDYFSKRQELIKPIQDKVFNAMQVVASRRSYIFVFDKANQSNLVYADSKYDISD
                     EVLRELKIKE"
     gene            complement(1885..4503)
                     /gene="bamA"
                     /locus_tag="PKN22_00550"
     CDS             complement(1885..4503)
                     /gene="bamA"
                     /locus_tag="PKN22_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNS41219.1"
                     /translation="MNLRLLLLLFFTGFIINVSISQIQIGGEEEVLVEDTTKITPTNN
                     EAISVGMDYNNPKEYIIGPINVEGAEGYDPNAIRLVAGLRQGQKIVIPGQQIASAINN
                     LWKEELFSDVQIYADKELAGVIYLTIKVAPRPKLSKYKFIGINKRQADKIREQINLFT
                     GKTISENLVYQTRSKIMGYFREEGFYAAKVNIKRVPDEIMMNSEMFLIECNRGHKVKI
                     KKINFYGNKTVKSGKLRRAMKDTKQRSIFRIFKASKFTESAYERDKIAMLNKFGEVGL
                     RDARIVKDTFYMVDDRNLMIEIKIDEGEKYYFGDINWIGNTKFRSSYLDTILGIKSGD
                     IYNKQLLDERLRMSQDGRDISSLYMDRGYLFFDVNAVETDFTDNKISYEIRIREGKEA
                     RVGTISIRGNTKTNDHVILREIRTRPGDLFSRNDIIRTQRELAQLGYFNEQAFQVNPM
                     PDPQKGTVDIEYVVEEKSSDQIELSGGYGGRNYITDRPMIIGTLGLTFNNFSVKNMFK
                     GEAWKPLPSGDGQRLSIRGQTNGRYYQSYNFSFTEPWLGGKKPNSLTFYLSHTALTNG
                     RLKKHAEYQGIGITGVGLALGRRKKWPDDYFTESFDISYQYYDLFRYAQLFPDLQEGY
                     SNDISFQYAIQRSSVSSPIYPTSGSTISLSAKSTLPYSLWEKNDYSTMTPQQRFKYLE
                     YFKIKLGLEWFFPLTHDQKLVLRTRVGMGYMGMYNKAKGLSPFERYYLGGSGISGMNQ
                     IGGRDVIAVRGYDDNALSSSAGDPIAIKYTMELRYPISLNPSATFFVLAFAEAGNSFP
                     SFKRFNPLDVKRSVGAGIRIFLPMFGMLGIDYGFGFDKLSPWATGYGQTSDQSIKQKG
                     YYGKLTFTIGMNLGEL"
     gene            complement(4500..5249)
                     /locus_tag="PKN22_00555"
     CDS             complement(4500..5249)
                     /locus_tag="PKN22_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNS41220.1"
                     /translation="MVNNTKIDLSKTPKHIAIIMDGNGRWAKQQGEQRLFGHNVGVES
                     VREVLKGAKELGVQYLTLYAFSTENWNRPKEEVDGLMDLLVRTIANEVKELHDSGVRM
                     LSIGDINGLPEGCRNELLAAQEHTKNNKEINLVLALNYSAKWEIVNATKKIAQAVAAG
                     TLKEEEITDEVFASYLTTAGIPDPELLIRTSGEHRLSNFLLWQSAYTEFYFTPILWPD
                     FKKENLFEAVYDYQNRERRFGKVSEQLNNEL"
     gene            complement(5387..5677)
                     /locus_tag="PKN22_00560"
     CDS             complement(5387..5677)
                     /locus_tag="PKN22_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41221.1"
                     /translation="MQLEIITPEANVFAGDANAVQLPGLDGLFQVLNGHAPIISALKE
                     GVVKVDLAADFSTNEKTSDLIVKDPKDSKIIRIAIKGGVAELMNNKLIVLAE"
     gene            complement(5700..7205)
                     /gene="atpD"
                     /locus_tag="PKN22_00565"
     CDS             complement(5700..7205)
                     /gene="atpD"
                     /locus_tag="PKN22_00565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNS41222.1"
                     /translation="MSAIKGKIAQVIGPVVDVSFDKGVELPKIYDALEVVKADGSKVV
                     LEVQAHVGENAVRAISMDSTDGFMRGLEVVSTGDAIKMPTGDAIKGRVFNVVGDSIDG
                     IGELDRSKGLPIHRPAPKFEDLSTSTEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGK
                     TVLIQELINNIAKGHGGLSVFAGVGERTREGNDLLREMLESGIIKYGDDFLHSMEKGG
                     WDLSKVDTNQLKESKATFVFGQMNEPPGARARVALSGLTLAEYYRDGEGDGQGKDILF
                     FVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGAMQERITSTKNGSITSVQAVY
                     VPADDLTDPAPANTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILTPEIVGEAHYN
                     CAQRVKVTLQRYKQLQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTGI
                     PGVLVDIQETIVAFNDILDGKYDKYPEAAFNLKGGIQDVIDAGEKMLAETH"
     gene            complement(7325..8152)
                     /locus_tag="PKN22_00570"
     CDS             complement(7325..8152)
                     /locus_tag="PKN22_00570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNS41223.1"
                     /translation="MRKIVAYLFQWIFKIPFCRNYFFGIHQRVFQPYNLFKNVKVSYH
                     YQGYKLQLHLEDWIQQCLFFTGEYEKAELKVLTHLLKDDSVFIDIGANIGLYSLRATD
                     KIKEKGKVIGFEPFIENFKQLKKHVVSNNLSTIITIENVAIGESEGKLQLYYSDKIQN
                     RGMVSAVYQENAKSYEVEMMRLDDYCAHKNINTINCVKIDVEGYEMNVLKGMTNVLLQ
                     FQPILLLEILEENTEQIESFLSNFGYVKYYISDNGFVVKENNNPNRKNYIFSVASLA"
     gene            complement(8156..8860)
                     /locus_tag="PKN22_00575"
     CDS             complement(8156..8860)
                     /locus_tag="PKN22_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS41224.1"
                     /translation="MAGKLYMIPTTLGGEQVNDVIPQTVQQLIAGLRYFIVEDIKSAR
                     RYLRRVDRNFPIDDSVFFELNKRTDEKSIISYLKPAKDGHNIGVISEAGCPGVADPGA
                     EVVELAHEIGIRVAPLVGPSSILLALMGSGFSGQEFTFHGYLPKERKERIKLLKDFEA
                     NTRRTGYTHIFMDTPFRNMNVLDDLLNELADTTKLCIASNITLPDESVWTMTVAQWRE
                     KAYDLSKKPTMFLIGK"
     gene            complement(8850..9314)
                     /locus_tag="PKN22_00580"
     CDS             complement(8850..9314)
                     /locus_tag="PKN22_00580"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNS41225.1"
                     /translation="MKVLMVCLGNICRSPMADGLLRQKVRQEGLEVEVDSAGTSNYHI
                     GDAPDSRMRATAKKLGCPIDDLRARQFTVADFDDFDLIYAMDASNLENILKLKRSTAD
                     EKKVKLILNELYPGENREVPDPYFGGEQGFIDVFNMLDKATDIILNNLKNGR"
     gene            9418..9612
                     /locus_tag="PKN22_00585"
     CDS             9418..9612
                     /locus_tag="PKN22_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNS41226.1"
                     /translation="MKIKKCQRCKVDFECNAEDIVNCFCNTITLSVNEQPKEATSYTD
                     CLCKKCLLEMDQNTSELTKK"
     gene            9638..10648
                     /gene="corA"
                     /locus_tag="PKN22_00590"
     CDS             9638..10648
                     /gene="corA"
                     /locus_tag="PKN22_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNS41227.1"
                     /translation="MNTSFGQIYTYNAAACTKEKFKTAQFVESFSLDALPKDKITWIN
                     FYMYDDLAPIEAFCKKAKLDSLVFQNIRDRDYRPQFEDYDHYLFFSVRSATPTTGKMT
                     RIFEEQLSFVLGEHYLISFQSKPSDYFGSVRDRLENGKGLLREKGAELLLYRLLDSIV
                     DNYFYALEGFTTEISELESKVSKSSTPEILSFIESHKRKHIEFRRIVAPLKEIAISLE
                     NSENKLFKRETKHYFSDLKQNCISITEEVESNKNALEGLANLYYAVQGQRMNEIMKML
                     TIVSTIFIPLTFLAGIYGMNFDNMPELRTKYGYFATWGVMVLIAVGLLYYFRRKGWLG
                     RK"
     gene            complement(10649..12166)
                     /gene="gpmI"
                     /locus_tag="PKN22_00595"
     CDS             complement(10649..12166)
                     /gene="gpmI"
                     /locus_tag="PKN22_00595"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNS41228.1"
                     /translation="MTNHFGLIILDGWGIGDRSKSDAVYNANTPFMDSLMANYPNATL
                     LTSGENVGLPDGQMGNSEVGHMNIGAGRIVYQELTRINKSIRDKEFFTLPTLVDAFQF
                     AKQNNKKVHFIGLVSKGGVHSTQEHLYALCQMAKSYGLSDVFIHAFTDGRDCDPKSGQ
                     KFLEELEQELQTSVGKVASVIGRYYAMDRDKRWERIKKAYDLLVNGQGAAFSSTQEVF
                     EHSYSNNITDEFIEPAVIIENGKPLATIEEGDVVINFNFRTDRPREISIALTQQDFHE
                     YNMHKLNLYYVTMTNYDATFENVRVVFEKDNLSNTLGEVIANSGKTQVRIAETEKYPH
                     VTFFFSGGREDAFANESRILVNSPKVATYDLQPEMSAVEVKDKICAAINTSQPNFICL
                     NFANPDMVGHTGVYEAIIKAVETVDSCLKEVVETGVKYGYEFLVIADHGNADFAINAD
                     GTPNTAHSLNPVPVVLVTNDKSVQLKSGILADVAPTILKRMGIEASKEMTGNVLV"
     gene            complement(12187..13074)
                     /locus_tag="PKN22_00600"
     CDS             complement(12187..13074)
                     /locus_tag="PKN22_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS41229.1"
                     /translation="MKRKLSIFGYFLSIYVLAQFFWWGYHIVDLTTQLSGDTAASNKN
                     ILMIVGEGVIFLSILVFGIIKIRNSVANELIVSRNQNNFLLSVTHELKTPIASIKLYM
                     QTLQKRNLSEEKRNEIIEKVLQQNEQLALLIDNILNATRLENKKLIANKEQLEVSKFL
                     DAIVERYKRQYPQIEFVNQVKKNISEKIDPFIFETIVNNLIENAIKYGASGGKITVEL
                     QKDTALKLRVLDNGPGIPAQFHKDIFKKFYRVGNEETRTQRGSGLGLFIVKELAHLHK
                     GKIKYYNNQPTGAVFELTL"
     gene            complement(13406..>14511)
                     /locus_tag="PKN22_00605"
     CDS             complement(13406..>14511)
                     /locus_tag="PKN22_00605"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41230.1"
                     /translation="ITNSTIANNSAFNGGGMYNYSSSSPTITNSTIANNSAYYGGGIR
                     NNSSSPTITNSTIANNSANSIGGGMYNYYSSPTINNSILFGNTAPNNAVMLSGNNSTP
                     IVKNSLIEGVSWNATIWGTDGGGNILSTTNPFTNSATGDFTLNSSSVAINAGNNALYN
                     GNINSDLDLGGNPRLNGVNIDMGAYESLIVVPLATYLLEFNAYCENKTRIEWQTASEL
                     NVSYFEIQYTREANNWKTIAQIPASGNVSGKKYEYYTNSQEELSYYRLCAVDKDGSIQ
                     IFSPIVVACNQDNNDWMVFPNPANNFITIQHPSETIQDILLLDNFGRTVLQTKIDARD
                     TFTFDVSNIKNGLYILQITTNQGATTKKIKINH"
BASE COUNT         4378 a         2805 c         2405 g         4923 t
ORIGIN      
        1 actcaaccgt ccaatgaaag gcacggttat ttgcattcct atcttcaata tttttggttt
       61 tctcaactta aaacgagaat tacccgatgg tagagatttg aatagaagtt ttccaggcac
      121 gcccaacggt tcattagcat cgcaatttgc ttttcaattt atgaaagaaa tagcacccgt
      181 tgtagatgtg attattgact ttcatacggg tgctgcacaa cgcaataatc acccccaaat
      241 tcgctgtgat tttaacgatg cagaaagttt ggaattggcc aaagttttta actcaccaat
      301 cattttaaac tctggtttaa tttcaaagac cattcgtgaa gtaattcaaa aattggggaa
      361 acaatacctc atttttgaag gcggaaaagc aaattctatt gaggaattgg tggtggatga
      421 aggcattaat ggtgccaaaa gaatactctc tcattttgga atgcgtagtt ttaaaatcga
      481 catatcattg ggaagagaac ctatgttttt aacagaatct aaatggatgc gagcttctaa
      541 ctccggttta tttctaccgc acgttaagaa tggaacgcgt gtaaaaaaag gtgagattat
      601 gggtattatt atggatcctt ttggaaaaat tgaacgcaac ataaaagcac aaaaagatgc
      661 tgtagtatta tgcgttaacg aagctccaac tgtttataaa ggcgatgcaa tttttcacat
      721 tgccattgaa gcataaaaaa aggctttccg atttgaaaag cctttaaatt taatatttga
      781 ttatcactat tttttagcct cgttttctaa cttcaataat tcaacagcta ctacacttgt
      841 aatatccatt gttttatcgt ggtaaagtgt tgtagactta tccactacca ttgttaattt
      901 ttgacgttct gcaatgatag caattgcttt ttgcactttg tctaaaattg gaatgttcaa
      961 ctcttcacca taagcttgca attcttgttg gatagtttct tgacgctctt gaacgcttct
     1021 ttctttatcc atgatttttt tctcagcagc ttgacgtaaa actggtgtta aatcaccttg
     1081 ttttgcttgg tattcagcat acgatttttc taaatcagca cgaagcaagt taaattcatc
     1141 agccaatgtt ttttcgtgtg ctttgtattt ttccaatgca gcttttcttg atggcattgt
     1201 atccaataat ttttgtgaat ctacaaaacc aattttaact tgggccatag ctccaaagct
     1261 taccatcatc aataatccaa ctaagatttt tttcatgttt gttaatttta atttttactc
     1321 tttaattttt aattctctaa gcacttcatc acttatatca tacttactat cggcataaac
     1381 caaattactt tgatttgctt tatcgaagac aaagatataa cttcttctgg aagccacaac
     1441 ctgcattgca ttaaatactt tatcttgaat aggtttaatt aattcttgac gttttgagaa
     1501 ataatcgccc tctactccaa atcgttgacg ttgcatttgc atggcttcag attccattgt
     1561 ttcaatttct ttcaatcggt ttgccttttc ctcgcttggt aaaagtactt cttctcgtgc
     1621 gaagttgtct tttttagttt ttatagcagc atatcgttct tctatttctt tggtccaacg
     1681 atctgccaac ttattcaact gttcttttgc atccgtgtat tctggaattt tctctagaat
     1741 aaaatccgaa tcaatgtatg cgtacttttg tgcagaggca aaacctgtgc caaatacaaa
     1801 tgctaaaata tatagtactg ttttcatttt aataattttg ctggtcataa cgtgcaaata
     1861 acggaattta ttgtgaatta acaattataa ctcacctaaa ttcataccaa tggtaaaggt
     1921 taacttacca taatatcctt tttgcttgat agattggtca cttgtttgtc cataacctgt
     1981 agcccacgga ctcaatttat caaatccaaa tccatagtcg atacccaaca taccaaacat
     2041 tggcaagaag atacgaatac ctgcaccaac agagcgtttc acatctaatg gattaaagcg
     2101 tttgaagctc gggaaactat ttccagcctc tgcaaatgcc aatacaaaga atgttgctga
     2161 tggattcaac gaaataggat aacgcaactc catggtatat ttaatggcaa ttggatctcc
     2221 ggcagatgaa gataaagcat tatcatcgta tccacgaaca gcaatcacat cgcgaccacc
     2281 aatttggttc atcccagaaa taccactacc tcctaaataa taacgttcaa atggcgacaa
     2341 cccttttgct ttgttataca tgcccatata gcccatccct actcgtgtgc gcaacaccaa
     2401 tttttggtcg tgtgttaacg ggaagaacca ttccaatcct aatttaattt taaaatattc
     2461 caaatactta aaacgctgtt gaggcgtcat tgtagaataa tcatttttct cccacaacga
     2521 gtagggcaat gtagattttg ctgacaatga aatagtactt cctgatgttg gataaattgg
     2581 tgaactaacc gaactacgtt gaatagcata ttggaaagaa atatcgttgg aataaccttc
     2641 ttgtaaatcg gggaacaatt gggcataacg aaataaatcg taatattggt aggatatatc
     2701 aaatgattct gtaaaataat catccggcca tttttttctt cttcctaaag ccaaaccaac
     2761 accagtgatt ccaatacctt gatattcggc atgttttttc aatcttccgt tggttaaagc
     2821 agtatgcgat aaatagaacg ttaatgaatt tggtttttta cctcccaacc aaggctctgt
     2881 aaatgagaag ttataggatt ggtaataacg tccgtttgtt tgtccacgaa tagataaacg
     2941 ttgtccatct ccagaaggta acggtttcca agcttctcct ttaaacatgt tttttacaga
     3001 gaagttatta aaggttaatc ccaaagtacc aataatcatt ggacgatctg tgatgtagtt
     3061 tctaccacca taacctccag ataattcaat ttggtcagat gatttttctt ctactacgta
     3121 ttcaatatca accgttcctt tttgtggatc tggcattgga tttacctgaa atgcttgctc
     3181 gttaaaatag cccaattgtg ctaattctcg ctgtgtacga atgatgtcgt ttcggctgaa
     3241 taaatcacct ggacgtgttc tgatttcacg caaaattaca tggtcgtttg ttttggtgtt
     3301 tccacgtatt gaaattgttc ctacacgtgc ttcttttcct tctcggatgc gaatttcata
     3361 gctaattttg ttatctgtaa aatctgtttc aactgcattt acatcaaaaa acaaataccc
     3421 tctatccatg tatagtgaag agatatcgcg accgtcttgg ctcattctta agcgttcatc
     3481 caacaattgt ttattgtaaa tatcgcccga tttaattcct aaaatagtat ctaaatacga
     3541 cgagcgaaat ttggtatttc caatccaatt gatgtctcca aaataatatt tttctccttc
     3601 gtcaattttg atttcaatca tcaagttgcg atcatccacc atgtagaatg tatctttaac
     3661 aatgcgtgca tctcttaaac caacctcgcc aaatttgtta agcatggcaa ttttatcacg
     3721 ttcataagcc gattctgtaa atttagatgc tttgaaaatt ctaaaaattg aacgttgttt
     3781 ggtatccttc attgctctac gcaatttacc agatttaact gttttattgc cgtaaaaatt
     3841 tatctttttg attttaactt tgtgtccacg attacattca attaaaaaca tttcagagtt
     3901 catcataatt tcatctggaa cacgtttaat gttcactttt gctgcataaa aaccttcttc
     3961 tctaaaataa cccatgattt tagaacgtgt ttgatacacc aagttttcag agatggtttt
     4021 accagtgaaa agattaattt gttccctaat tttatctgcc tgacgtttgt taattccaat
     4081 aaatttgtat ttagataatt ttggtcgagg cgctacttta attgttaaat agataacacc
     4141 tgcaagttct ttatcggcat aaatttgtac atcagaaaac aattcttctt tccacaagtt
     4201 attgatagct gaagcaattt gttgacctgg aataacaatt ttttgtcctt gtcgcaagcc
     4261 cgccaccaaa cgaatggcat tcggatcata gccttctgct ccctccacat tgattggacc
     4321 aataatgtat tctttgggat tattgtaatc cattccaaca gaaatagctt cattgttggt
     4381 aggagttatt tttgtagtat cttcaaccaa cacttcttct tcgcctccaa tttgaatttg
     4441 ggaaattgaa acattgatga tgaaacctgt aaaaaataat aagagcaaaa gtcttaagtt
     4501 catagttcgt tatttaattg ttctgatact ttaccaaatc ttctttcacg attttggtaa
     4561 tcgtaaacag cttcgaataa gttttctttt ttaaaatcgg gccataaaat tggcgtaaaa
     4621 taaaattctg tgtaggcact ttgccataaa aggaagttgc tcaagcgatg ttctcccgat
     4681 gtgcgaatta acaattcggg atctggaatt ccagccgtag ttaaatacga cgcaaacacc
     4741 tcatctgtaa tttcctcttc ttttaatgtt ccagcagcaa ccgcctgtgc tatttttttg
     4801 gtggcattta caatttccca tttagcagaa tagttgagtg ccaataccaa attaatttct
     4861 ttgttatttt ttgtatgttc ttgcgcagct aataattcgt ttctacaacc ttctggcaag
     4921 ccattgatat caccaatgct caacatgcgc acacccgaat cgtgcaattc tttcacttca
     4981 tttgcaatgg ttctcactaa taaatccatc aaaccatcca cttcttcttt tggacggttc
     5041 caattttcag tagaaaatgc ataaagtgtc aggtattgca cgcctaactc tttagctcct
     5101 ttcaaaactt cgcgcaccga ctcaacgccc acattgtgac caaacaagcg ttgctcgcct
     5161 tgttgtttag cccagcgacc gttgccatcc atgataattg cgatgtgctt tggtgttttt
     5221 gacaagtcta ttttcgtgtt atttaccatt ctttagtgaa accaaacgac gaatttaatg
     5281 aaagtattgt ttatataaaa cgtttttttg ataaagctta acatacctta acaaaaaaag
     5341 agggattagg tttccccatc cctcttctaa aagaactatt tttttattat tctgcaagaa
     5401 cgataagctt attgttcatc aattctgcaa caccaccttt gatagcgatg cgaataattt
     5461 ttgaatcttt tgggtctttt acaattagat cacttgtttt ttcgtttgtt gaaaaatcag
     5521 ctgctaaatc tactttcaca acaccttctt tcaaggcaga gataataggt gcgtgaccat
     5581 tcaatacttg aaacaagcca tctaatcccg gtaattgcac tgcgtttgca tctcctgcaa
     5641 aaacatttgc ttcgggtgtt atgatttcta attgcatttt tttgttttta acgaattaat
     5701 taatgcgttt cagccaacat tttttcacca gcatcgataa catcttggat accacctttc
     5761 aagttgaaag ctgcttctgg atatttatcg tatttaccgt ccaaaatatc gttgaatgct
     5821 acaattgttt cttgaatatc taccaataca ccagggatac ctgtaaattg ctctgcaacg
     5881 tggaatggtt gagataagaa acgttgtaca cgacgcgctc tatgcaccac caatttatct
     5941 tcttcagaca actcatccat acctaagata gcaatgatgt cttgcaattg tttgtaacgt
     6001 tgtaatgtaa ctttaacacg ttgagcacag ttgtagtgcg cttcaccaac gatttctgga
     6061 gtcaagatac gagaagttga atccaatgga tccactgcag gatagatacc taactcagca
     6121 attttacgag acaatacggt tgttgcatcc aagtgagcaa atgtatttgc aggagcaggg
     6181 tcagttaagt catccgcagg tacgtaaacc gcttgcacag atgtaattga accattttta
     6241 gttgacgtaa tacgctcttg cattgccccc atctcagttg ccaatgttgg ttggtaacct
     6301 accgcagatg gcatacgtcc aagaagtgct gatacttcag atcctgcttg cgtgaaacgg
     6361 aagatgttgt caacgaagaa aaggatatct ttcccttgtc catcaccttc accatcacgg
     6421 taatattctg ctaatgtcaa accagacaat gccacacgtg cacgtgcacc tggaggctcg
     6481 ttcatctgac caaacacgaa tgttgctttt gattctttca attggttagt atctactttt
     6541 gacaagtccc aaccaccttt ttccattgaa tgtaagaaat catcaccata tttgataatt
     6601 ccagactcca acatctcacg caacaagtca tttccttcac gagtacgctc accaacacca
     6661 gcaaatactg acaaaccacc gtgtcctttt gcgatattat taatcaactc ctgaatcaat
     6721 actgttttac ctacaccggc accaccaaac aaaccgattt tacctccttt tgcataaggc
     6781 tcaatcaagt cgattacttt aatacctgta aacaaaactt ctgttgaagt agaaaggtct
     6841 tcaaattttg gcgctggacg gtgaataggc aaccctttag atctatccaa ttctccaata
     6901 ccatcaattg aatcaccaac tacgttaaat actcttcctt taattgcgtc accagtaggc
     6961 attttaatag catcacctgt actcactact tctaagcctc tcatgaaacc atctgttgag
     7021 tccattgaga ttgcacgcac agcattttca ccaacgtgtg cttgaacttc taaaactact
     7081 ttagacccat ctgctttaac aacttctaat gcgtcataaa tctttggaag ctccactcct
     7141 ttgtcgaagc ttacatcaac tacagggcca attacctgtg ctattttacc ttttattgct
     7201 gacattgata cctatatttt aagtatatta tttccaattt tgggtgcaaa tatagtttat
     7261 ttattttgta tcttaataat aaacctatta aaatttacta aaaaatattc ttagttatgc
     7321 atatttaagc aagtgatgca acagaaaaaa tatagttttt ccgattagga ttattatttt
     7381 ccttaacaac aaatccatta tcggaaatat agtattttac ataaccaaaa ttggataaaa
     7441 atgattcaat ttgttctgtg ttctcttcca aaatttccaa cagtaaaatt ggttgaaatt
     7501 gcagcaacac atttgtcatt ccttttaata cgttcatttc atatccttca acatcaattt
     7561 taacacaatt tatagtgttg atgtttttgt gagcgcaata atcatccaaa cgcatcattt
     7621 ctacttcata gcttttagca ttctcctgat acacagccga aaccatgcct ctattttgga
     7681 ttttatctga ataataaagc tgtaattttc cttccgattc tccaattgct acattctcaa
     7741 tagttataat tgttgagaga ttgttgctaa ccacatgttt tttaagctgt ttaaaattct
     7801 caataaaagg ctcaaaacca ataacttttc ctttttcttt tattttatcg gttgctctta
     7861 atgaatacaa cccaatatta gccccaatat caataaaaac actgtcatcc tttaatagat
     7921 gtgtcaagac ttttaattct gctttttcgt attctccagt aaagaacaag cattgctgaa
     7981 tccaatcttc taaatgtaat tgcaacttat atccttggta atggtatgaa acctttacat
     8041 ttttgaataa attgtagggt tgaaatacac gctgatgaat accaaaaaaa tagtttctac
     8101 aaaacggtat tttaaaaatc cattgaaata ggtatgctac aatttttctc attttttatt
     8161 ttccaatcaa aaacatggtt ggttttttac tcaaatcgta tgctttttct ctccattgag
     8221 caacggtcat agtccacaca gattcatcgg gcaacgtaat attggaagcg atgcataact
     8281 tggtggtgtc agccaattcg ttcaataaat cgtctaaaac gttcatattt ctgaagggtg
     8341 tatccataaa aatatgagta taaccagtgc ggcgtgtatt ggcttcaaaa tcttttagca
     8401 acttgatgcg ctctttgcgt tctttgggta aataaccgtg aaaggtaaac tcttgtcccg
     8461 aaaaaccgct acccattaaa gctaataaaa tggaagaagg gcccactaac ggtgcaacac
     8521 gaataccaat ttcatgtgcc aattcaacaa cttctgctcc tgggtcggca acacctggac
     8581 aaccagcctc tgaaattaca ccaatattat gaccatcttt tgcaggcttt aagtaggaaa
     8641 tgatgctttt ttcatcggta cgcttgttca attcaaaaaa aactgagtca tcaattggga
     8701 aattacgatc tacgcgacgc aaataacgac gtgctgattt gatatcttca acaataaaat
     8761 agcgtaaacc cgcaattaat tgttgaactg tttgtggaat tacatcgttt acttgttcac
     8821 cgcccaacgt tgtaggaatc atgtaaagtt tacctgccat tttttaaatt atttaaaata
     8881 atatctgttg ctttgtccaa catgttaaat acatcaataa atccttgttc gccaccaaag
     8941 tatggatcgg gaacttctct attttctcca ggatacaatt cgttgagaat aagttttact
     9001 tttttctcat cggctgtgga gcgttttaat tttagaatat tctccaaatt gcttgcatcc
     9061 atggcataaa tcaaatcaaa atcatcaaaa tctgctactg taaattgacg agctcgcaag
     9121 tcatcaatgg ggcaacctaa ttttttggca gttgctcgca tacggctatc gggtgcatca
     9181 ccaatgtgat aattggatgt tccagcagaa tcaacctcaa cctccaatcc ttcttgtcgt
     9241 actttctgac gcaacaatcc atcggccatt ggggaacgac aaatattgcc caagcaaacc
     9301 attaaaactt tcattttctt taattttatt gcgtaaagta gaaatattct gcttcacaaa
     9361 acaaaagtag agataaattt gcattgaaag aaatttatag ttgtttagtc ctctaaaatg
     9421 aaaataaaaa aatgccaacg gtgcaaggtt gactttgaat gcaacgctga agatattgtc
     9481 aattgttttt gcaatacgat tactttgtcg gtgaatgaac aacccaaaga agcaacaagt
     9541 tataccgact gtttatgcaa aaaatgcttg ttagaaatgg accaaaacac gagtgagtta
     9601 accaaaaaat agtaatttta atcccaacag ttgaactatg aacacatcat tcggtcaaat
     9661 atatacgtat aacgcagcgg catgcaccaa agaaaaattt aaaacggcgc aatttgttga
     9721 atcattttca ttggatgcgc ttccaaaaga taaaattacg tggattaact tttacatgta
     9781 tgatgatttg gcgcctattg aagcattttg taaaaaagca aaattagact cgttggtttt
     9841 tcaaaacatt cgcgatagag attaccgccc acaatttgaa gattacgacc attatttgtt
     9901 tttcagtgtg cgttcggcaa cgccaacaac gggaaaaatg acacgtattt ttgaagagca
     9961 gttgagtttt gttttgggcg agcattatct aatttccttt caatccaaac catccgatta
    10021 ttttggcagt gtgcgcgata gacttgaaaa tggtaaaggg ttgctgcgcg aaaaaggtgc
    10081 ggaattgttg ttgtatcgcc ttttagactc cattgtagat aattactttt atgctttgga
    10141 gggatttacc accgaaataa gcgagttgga atcaaaggtg agtaaatctt caactcccga
    10201 aatattatcg ttcattgaat cacacaaacg caaacacatt gagtttagac gcattgttgc
    10261 acctttaaaa gaaattgcta tttccttaga aaactctgaa aataaattat ttaaacgcga
    10321 aaccaagcat tatttttctg atttaaagca aaattgcatc agcatcaccg aagaggttga
    10381 atccaacaaa aacgcattgg aaggtttggc caacttgtat tatgctgttc aagggcaacg
    10441 catgaatgaa atcatgaaaa tgttgaccat tgtgagcacc atattcatcc cgcttacctt
    10501 tttggcagga atttatggga tgaactttga caatatgccc gaattacgca ccaaatatgg
    10561 ctattttgcc acttggggag taatggtgtt aattgcagtt ggattgctct attatttcag
    10621 aagaaaagga tggttgggta gaaaataact acaccaacac gtttcctgtc atttctttgg
    10681 atgcttcaat gcccattctt tttaagatgg ttggagcaac atcggctaaa attccgcttt
    10741 ttaattgaac acttttatcg ttggttacca acacaactgg caccggattg agggagtgcg
    10801 cagtattagg cgttccatca gcgttgattg caaaatcggc atttccatga tcggcaataa
    10861 ccaaaaattc gtatccatat tttacacctg tttcaaccac ttcttttaag caactatcca
    10921 ccgtttcgac cgctttgatg attgcttcgt aaacaccagt gtgaccaacc atatcgggat
    10981 tagcaaaatt taaacaaatg aaattaggtt gagaagtatt gatggctgca caaattttat
    11041 ctttcacttc aacagcactc atttctggtt gcaaatcgta ggttgccact tttggtgagt
    11101 tgactaaaat acgactttcg tttgcaaatg catcttcacg accgccactg aagaaaaatg
    11161 taacgtgcgg atatttttct gtttctgcaa tgcgcacttg tgttttcccg ctgttggcaa
    11221 tcacctcgcc caaggtattg gaaagattgt ctttctcaaa aacaacacga acgttttcaa
    11281 aagttgcatc gtaattggtc attgtaacat aatacaaatt taatttatgc atgttgtatt
    11341 cgtgaaaatc ttgctgtgtt aaagcaatgg aaatttctct tggtcggtcg gttctaaaat
    11401 taaaattaat caccacatcc ccttcttcga ttgttgccaa cggttttcca ttttcaatga
    11461 ttaccgctgg ttcaatgaat tcatcagtaa tgttgttgct atacgagtgt tcaaaaactt
    11521 cctgtgttga agaaaacgca gctccttgcc cgttgaccaa taaatcatac gcttttttga
    11581 tgcgttccca acgtttatct ctgtccatgg cgtagtagcg accaataacc gaagccactt
    11641 taccaactga ggtttgcaat tcttgctcta attcttccaa aaacttttgt ccacttttag
    11701 ggtcacaatc gcgtccatcg gtaaaagcat gaataaacac atccgacaaa ccataacttt
    11761 ttgccatttg acataaagca tataaatgtt cttgcgtgct atgaacacca ccttttgaca
    11821 ccaagccaat aaagtgtact tttttgttgt tttgtttggc aaattgaaat gcatccacca
    11881 aggttggaag agtaaaaaat tctttgtctc gaattgattt attgatgcgg gttaattctt
    11941 ggtaaacaat tcttccggca ccaatgttca tgtgtcccac ttccgaattt cccatttgtc
    12001 cgtcaggtaa ccccacattt tcaccactag ttagcaaagt tgcgttggga taatttgcca
    12061 tcaaactatc cataaaaggt gtattggcat tgtacacggc atccgatttg gagcgatcgc
    12121 caattcccca tccgtccaaa attattaatc cgaagtgatt tgtcattttc ttttcgattt
    12181 tttcaattac aaagttaatt caaagaccgc acctgttggt tggttgttgt aatattttat
    12241 ttttcccttg tgcaaatgtg ccaattcttt tacgatgaac aagcctaatc cagagccacg
    12301 ttgtgtgcgt gtttcttcgt ttccaacacg gtaaaatttc ttaaaaatat ctttatggaa
    12361 ttgggcagga atgccaggac cattgtcaag aactcttagt tttaatgctg tatctttttg
    12421 caattcaacg gtaatttttc caccagaagc accatatttt atagcatttt caattaaatt
    12481 attgacgatg gtttcaaaaa taaaagggtc aattttttct gaaatatttt ttttaacttg
    12541 gttaacaaat tcaatttgtg ggtattgacg tttgtagcgt tccacaattg catctaaaaa
    12601 tttagacact tcgagttgct ctttgttggc gatgagcttt ttattttcca aacgagttgc
    12661 attaagaatg ttatcaatca acagggctaa ttgttcgttt tgctgcaata ctttttcgat
    12721 gatttcattg cgtttttctt cgcttaaatt tcgtttttga agcgtttgca tatagagttt
    12781 aatacttgca attggcgttt ttagttcgtg tgtaaccgag agtaaaaaat tattttggtt
    12841 gcgagaaaca atgagttcat tggccacgct gttgcgaatt ttaatgattc cgaaaacaag
    12901 aatggaaaga aaaatcaccc cttctcccac aatcataaga atatttttat tgctagctgc
    12961 ggtatctccc gaaagttgcg ttgttaggtc cacaatatga tagccccacc aaaaaaattg
    13021 agccaacaca taaatactga ggaagtagcc aaatatggat aattttcttt tcataaatga
    13081 agtgataagt gcaaaggtaa tggaaaagca attgacaaag aaaaattgct aggttttatt
    13141 aaaaaacgaa aaactcacca acatttttgc tgatgagttt tttcaacttg tttggtttag
    13201 ttttcgctac tttttaagtg aataacacaa gtggttggat ttaatattat tgtagtttgc
    13261 ttgttattaa aattgtgagt ttttaatttt cagcaattgc atttgccaat cttgaagcaa
    13321 aagtagagca acctctattt tttatcaaat tttagggtaa acaaactgta tatacctgat
    13381 acacaacatg ctgtatatta tttttttaat gattaatttt aatttttttg gttgttgcac
    13441 cttgattggt tgttatttga agtatataca aaccattttt tatgttggaa acatcgaaag
    13501 taaatgtgtc tcttgcatct atttttgttt gaagaactgt tcttccaaaa ttatctagta
    13561 ggagtatatc ttgaatggtt tcggatggat gttgaatggt tatgaaatta tttgctggat
    13621 tagggaaaac catccaatca ttgttatctt ggttacaagc tacaacaatc ggtgaaaaaa
    13681 tttgaatact tccatcttta tccacggcac acaatctata atagcttaat tcttcttgtg
    13741 aattggtgta atattcatac tttttacccg atacatttcc tgatgcaggt atctgtgcaa
    13801 tggttttcca attatttgct tcacgtgtat attgaatttc aaaataacta acgtttaatt
    13861 ctgaagcagt ttgccattct atacgggttt tattttcaca atatgcatta aattcaagaa
    13921 gataggttgc aagaggcaca acaattaaac tttcataggc tcccatatcg atattcacac
    13981 cattcaagcg tggatttcct cccaaatcta gatcggagtt gatgttacca ttataaagtg
    14041 cattattacc agcatttatc gcaacactac ttgaatttag tgtaaaatca ccggtagcac
    14101 tatttgtgaa tgggttggtt gttgatagaa tatttcctcc tccatctgtt ccccaaatgg
    14161 tagcattcca agaaacacct tctattaaac tgttttttac aataggagtg ctattgtttc
    14221 ctgaaagcat aactgcatta ttgggagctg tattcccaaa caaaatagaa ttattaattg
    14281 ttggtgatga ataataatta tacatcccgc cgccaatact attcgctgaa ttatttgcaa
    14341 tggttgaatt ggtaattgtt ggtgaagaag aattattacg tatcccgcca ccataatacg
    14401 ctgaattatt tgcgatggtt gagttggtaa tggttggtga tgaagaagaa taattataca
    14461 tcccgccacc attaaaagct gaattatttg cgatggttga gttggtaatg g
//
LOCUS       DAOQVQ010000008        14107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_4879_length_14107_cov_3.893538, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000008 DAOQVQ010000000
VERSION     DAOQVQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_14107_cov_3.893538"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKN22_00610"
     CDS             <1..830
                     /locus_tag="PKN22_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41231.1"
                     /translation="NITTVIANTAQGGHIRNLCLTAPSKINFFGIIHTIKMLNGSFTQ
                     TLISTKIKTYFVLNDTLKSRVKPQKGRTVHSFYPLSYAHFGKKLEKKTDEIWIGIIGG
                     VESRRKDLTGFLKMAQQTPSNVQFIFLGKSDAKKEEVAAFKQAIEQSNLTSRVQLFDD
                     FVSEVDFDAYLSHFDGLMPLVHPNTPSAEEYFSRQISGAINNAFAYKIPLMIHENYRD
                     WEDFNKGVEFYNLNNFGKQFSNFLQHHTQLKQQLIDNPKFSATTQNQQFAAIVMNGK"
     gene            complement(880..1239)
                     /locus_tag="PKN22_00615"
     CDS             complement(880..1239)
                     /locus_tag="PKN22_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41232.1"
                     /translation="MKTAQANQKLEAIIEKVEKKGIEAPQLVEDLKSLREIALQEQDP
                     LVTKVLRLTYEYLTANNAFSVEAQYDEDEDGNEFPVEVDDKENLLYLLNLLKNAEHKI
                     NREEIKDYRTALKEELY"
     assembly_gap    1406..1415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1472..3580
                     /locus_tag="PKN22_00620"
     CDS             1472..3580
                     /locus_tag="PKN22_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNS41233.1"
                     /translation="MEKVQFVIQHTAQSQRAVENTLKLLESGATVPFIARYRKELTGN
                     LDEVQITSIRDEAKKYDEIEARQQTILKSIEQQDKLTPELKEKITTTFDLVELEDLYL
                     PYKQRKETRGDKAKKAGLEPLAKMIMSQRGDLYAMANRFVKGSINDEDEAIQGAQDII
                     AEWVNESTAARARLRNLYTRKGKLSSKLVKGKEIEAEKYKDYFDFSEPLFKTASHRFL
                     ALYRADREGLLKITIQPEKEEVLETLHRMFVKGNDDCSAVVEKACDDAYKRLLAPSLQ
                     NEILQAQKEKADKEAIKVFSTNLRQLLLAPPLGEKRVLAIDPGFRTGCKVVCIDEHGS
                     LLTNATIYPHPPQKETSAAKAKIAQLVEAYKIEAIAIGDGTAGRETETFIKHIQFNRD
                     LLVFSVREDGASIYSASSIARKEFPDYDVTVRGAVSIGRRLMDPLAELVKIDPKSVGV
                     GQYQHEVNQTQLKEALDDVVVSAVNTVGVDLNTASPYLLSYVSGLGPQLAENIVAHRT
                     EVGKFTSREELKKVKRLGDKAFEQAAGFLRIREGKNPLDNSSVHPESYKHVEKIAKKL
                     GLKTEELIGNAEKLATIKQADFQEIDAYTFADIVNELKKPGRDPRKQAKVLEFDSSIR
                     TIEDLKIGMELEGIVTNVTAFGAFVNIGIKENGLIHKSQLADEYVEDPSKFIQLHEHV
                     RVKITEIDIPRKRVGLKRMK"
     gene            complement(3584..3946)
                     /locus_tag="PKN22_00625"
     CDS             complement(3584..3946)
                     /locus_tag="PKN22_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HNS41234.1"
                     /translation="MENRKCLECGTVLRGRKDQKFCSDQCRNTYNNRQNEDANKYVRR
                     INNILRKNRRILEKLNPKGKTTVDGIHLAEEGFNFHYFTNTYKTQKGTVYFFCYEQGY
                     QKIENDQYILVVKQDYVK"
     gene            4065..5075
                     /locus_tag="PKN22_00630"
     CDS             4065..5075
                     /locus_tag="PKN22_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS41235.1"
                     /translation="MNDSLSPAEALRQLSERISIEIAGINLLREEIAKVIVGQTYMVD
                     RLLVALLADGHVLLEGVPGLAKTLAIKTLADAIDVDFNRIQFTPDLLPADVTGTLVYS
                     PKNESFSVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGEETFALPQPFLV
                     LATQNPIEQEGTYPLPEAQVDRFMLKVFMDYPSMEEERMIIRSIVRPEGLPQAKQVIK
                     ASDIVRARSLVKEIYMDEKIEKYIVDLVFATRRPSQFGLEFLEPMIAYGCSPRASINL
                     ALAAKAYAFLQGRAFVIPEDIRAIAPDVMRHRIGLTYEAEAENLTAAQIVSKIISKVE
                     VP"
     gene            5087..5953
                     /locus_tag="PKN22_00635"
     CDS             5087..5953
                     /locus_tag="PKN22_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNS41236.1"
                     /translation="MEAQELFQKIRRLELKAKGLTKHIFSGEYHSAFKGRGMAFSEVR
                     NYQVGDEIRTIDWNVTARFNDPFVKVFEEERELTVMLVVDVSNSADFGSTATSKKEML
                     LETMAILAFSALSNNDKVGVIFVSDTVERYLPPKKGRQHILYLLREFIAFKAKNKGTN
                     LNNGLKFLKNTQKKRTICFVISDFIDESNYMEGLKIALKNHDVIALMLEDPSEYELPK
                     MGFLQLYNAETNATTWVDSNNPQVRATYKANFEANKQKITKEFTANGIDFAVLNTGSD
                     YFLELIKLFKNR"
     gene            5954..6910
                     /locus_tag="PKN22_00640"
     CDS             5954..6910
                     /locus_tag="PKN22_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNS41237.1"
                     /translation="MNGLKNKTIVRVLMFVLLTSTLSMCAVFGQELKATVNKDKVQLG
                     EPFELHLTARLAPNSTIKFVPAVSELAALQNDEKDIALEVTTAFSDSSNSTQEATIWT
                     GKYTLIAWETGKITIPIQKISINDSTYLFNATTVYVNPQTIGAKNELFDIQEGFSNVS
                     TDYTLLELLQKYWYFVAIALAAIAGLLWWWRKRKQKTVQPIVVLPLDVRALNAIDALD
                     NKKLWTKGQLKQHYTELSHILRSYMSGRYQLNLLETTTFETKALLKQVGVNEATISSY
                     LLLLKQADMVKFAQSSSDETTLINNSRLAKQLITSTTPTDIK"
     gene            6907..7938
                     /locus_tag="PKN22_00645"
     CDS             6907..7938
                     /locus_tag="PKN22_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS41238.1"
                     /translation="MTMPFNIRIWEYDFFAPHWLWLLLLLPLLYWYLHQKERKKQGFW
                     KFTGTVSNQQQLRSNYVEWVQKSILLIFCLVVGLFIIALAKPYNWNSADESNNNYQHG
                     IDIILALDISGSMLEQDFYPNRMEVAKKIAKEFVDGRKGDRIGLVAFAGAAYTSCPAT
                     TDYDVLKKQITQLNCYANIESGTAIGVGLGTAVTRLRNDSIPSKVIILLTDGSNNAGA
                     ISPQEAAELAKAKNIRVYTIGVGSEDGMIKIETPFGTIQQPVESDLDEATLQQIASIT
                     NAHYFRATDEESLRAIYKEIEKMEQRKIIDHYFQAEPPAMPKNFLIAALILALVGWSV
                     QQLLFKHHE"
     gene            7931..9076
                     /locus_tag="PKN22_00650"
     CDS             7931..9076
                     /locus_tag="PKN22_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS41239.1"
                     /translation="MNKPSTHTLKKFTLLILAWEVAFWLLFGGLFLVLNTPKGMGNSY
                     FSFENPEQLWWLWLLGPIIGLFLYTTYQSQHTLAVMGKKTRLTIVKTASGSKQLLRFF
                     LFRNAVVFLIIALAQPAFGTKKTAATIKSMELVVCLDISNSMNANDIDHSASRLDIAK
                     RAIMELINGLSGEKIGITIFANEAFTQLPLTLDYQAAKMFVQEIETNMISNQGTNIQK
                     ALENTYAFFSKNKKLSKAVLLITDGESQEGNPSTILERYNEDGIHLAVLGIGTQSGGL
                     VPIDPERPELGYKRSAQGVAVQSKLNPAMIQQIAAEANGIALISENAYPNLKGLLNDI
                     ANMERASTTGMDFEVKKQRYQIPLLIALACWLSYLLLPIVSLKKHVS"
     gene            9073..9789
                     /locus_tag="PKN22_00655"
     CDS             9073..9789
                     /locus_tag="PKN22_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41240.1"
                     /translation="MKLVMLSAFMVLTSVVFGQQWKDSLAAARKAYEQKNYSKALKLY
                     QSAQKNAPKDIDLSKEIGQATYKSSNYSDAETLFRQQASTEKSAIEKAKAYHNWGNSL
                     IQQKKYGEAIEAYKQSLRNDASNNKTRHNLSEAMRLRKEEEQKQDNQNNQNQNNQNQN
                     QNNQNQNQNNNKQQQNNKQNQTNNSENNHQKDNSNNSQLQDKAIEKKLDELMKQDAST
                     KRKLGQGKNPSGAPKSGNDW"
     gene            9789..11483
                     /locus_tag="PKN22_00660"
     CDS             9789..11483
                     /locus_tag="PKN22_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNS41241.1"
                     /translation="MKAVVVHICFLFCSLFVWGQKASVKLLVEPQEVSVNQQFSITLK
                     SNVDGNIVENWPSNFERGYGVQSMSRYVQDYSNGKMVQEHLVIFTGEFTKAGSYKIGP
                     FYVKAGNKTYTSNSVMVTVGGASNNSNGGEITKKQLNQPAFGVIEVSATKLYEGEPLV
                     VSGRVYAREETFGRPILKRPFNFNGVSDMHELQQTEMWEPKVVRNREYLSFSFGKQVL
                     FPVGSGRLDIQSFEIFLPFFDQNYNVVSSTPSVEIIPLPSNPPANFCGGVGEFSVEQK
                     IVNPQNVKQGDIVQIEVTVNGKGNLHAIESPVLQLPKGMNIYGDAETKDDFSFTSQGS
                     TGKKTYTYHVQVTQEGKQVIEPVAIAYFNPKTEKYATASAAKSITVDVLVNKAFSLQT
                     ENTATPQPVNEFDKKYNYSKLKSVLTSSNSWLWYGAGSLLLLAGLLFIFYPRKKKVVA
                     PTVEITPTIQIETTSATEVQEQIARLEFYAKEQAHDLFYAALEKTLLMVLNFHLQIDK
                     NNQLSRNELLSALRNKQPEKAIQIQRLFEKSDVSRYGMGFNAEEMESVVQEVKGIA"
     gene            complement(11536..>14107)
                     /locus_tag="PKN22_00665"
     CDS             complement(11536..>14107)
                     /locus_tag="PKN22_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNS41242.1"
                     /translation="GLPIATVELKNQFSGQNVSNAQKQYAFDRDQTEPIFQFKKRALV
                     HFAVDTDECYMTTKLAGTNTRYLPFNLGDQNGAGNPLNQNGYRTAYLWETIWAKDSFM
                     DIIGRFMHLSVEEIKIGGVKKIKETLIFPRLHQLQVVRELTQDARKHGAGKNYLIQHS
                     AGSGKSNSIAWLSYRLSSLHNDQNERIFDSVIVITDRKVLDSQLQNTIYQFEHKEGVV
                     QKIDKDSQQLANAISTGSNIIITTLQKFPFILDKIGALEARKYAVIIDEAHSSQGGEA
                     SKKMKEVLSAKSLEEAAAQELDSGLDEDAEDQIRKSMEARGQQQNLSFFAFTATPKNK
                     TVEVFGTPDKNGIPKPFHLYSMKQAIEEGFILDVLKHYTTYKTYYKLTKDIENDPNVN
                     KKAASRAIGRFMSLHPHNLSQKTEVMVEHFRNVVAKKIGGQAKAMVVTSSRLHAVRYK
                     EEFDKYIAEHHYSDIKTLVAFSGKVMYDTYPEGVTEAELNGFKEKELPKRFATDEYQL
                     LLVADKYQTGFDQPLLHTMYVDKKLSGVKCVQTLSRLNRTCAGKDDTFILDFVNEADA
                     ILESFQPYYELTTVQETTDPNRLYDLKTEIEKAQVIWKSEVDNFCNVYFKDVQILSKN
                     DQGKLNSYIDPAVDRYKKLPEETTQGDVVVDVSQENFKHALQSFVRIYSFLTQIMPFS
                     DVELEKLYAYSRYLHKKLPKKNQSERFQLNDEVSLEYYRLQKVAEQQILLEPKGEYGL
                     EGINNAGIRMTKDEKAALSEIITVINKRFGTEFTVADKLLFDQIEEDMVADKQLAAQA
                     KSNSIENFKYGFKEIAIDKFISRMDQNQDIFTRMMNDQDFNGLVMDYLLKKVYGRLSL
                     "
BASE COUNT         4573 a         2600 c         2717 g         4207 t
ORIGIN      
        1 aaaacatcac cactgtgatt gccaatacag cccaaggagg tcacatccgc aatttgtgtc
       61 taacggctcc atcaaaaatt aatttttttg gtattattca caccatcaaa atgctcaatg
      121 gcagctttac tcaaacactt atttctacta aaataaaaac gtattttgta ctcaatgaca
      181 ccttgaaaag tcgtgtaaaa cctcaaaaag ggagaactgt acattctttt tatccgttga
      241 gttatgctca ctttggtaaa aagttggaga aaaaaacaga tgaaatttgg ataggaataa
      301 ttggtggtgt tgaatcgcgc agaaaagatt taacgggctt tttaaaaatg gcgcaacaaa
      361 ctccgagcaa cgttcaattc atttttttgg gtaaaagcga tgcaaaaaaa gaagaagtgg
      421 cggcatttaa acaagctatt gaacaaagca accttacatc tcgggttcaa ttatttgatg
      481 attttgtatc ggaagtggat tttgatgctt acctatctca ttttgacgga ctaatgccgt
      541 tggttcatcc caatacacca agtgccgaag agtatttttc tcgtcaaatt tcgggggcta
      601 tcaacaatgc gtttgcctac aaaattccgt taatgattca tgaaaactac cgtgattggg
      661 aggattttaa caaaggagtt gagttttaca atttgaataa ttttggcaaa caattttcca
      721 atttccttca acatcacacc caattaaaac aacaattgat tgacaatcct aaattttcgg
      781 ctactacaca aaaccaacaa tttgcggcca ttgtaatgaa tggaaaataa aaaaatccgc
      841 tattgctaac ggatttcaaa attattgcat cgattacaat taatacaatt cttctttcaa
      901 agctgtacgg taatctttaa tttcttcgcg gtttattttg tgttctgcgt ttttaagcaa
      961 attcaacaaa tacaacaagt tttctttgtc atctacctca actggaaatt catttccatc
     1021 ctcgtcctca tcgtattgag cttcaacact aaatgcgttg ttggcagtca agtattcata
     1081 agtcaaacgc aacacttttg taaccaaagg atcttgctct tgcaaagcaa tctctcttag
     1141 cgattttaaa tcttctacta attgaggagc ttcaattcct ttcttttcaa ctttctcaat
     1201 aattgcttct aacttttgat ttgcttgcgc agtcttcatg tctttacttt taaaactctt
     1261 ttattcaaat aattaacctc actcaacaaa aattgaatga tttgatggta caaatgtaag
     1321 caacttaaat cataaaaaca taaatacttt cattttaatc taaaaaattg ttttaatatg
     1381 tgcggttaat ttttttattt attatnnnnn nnnnntgtta ataaattctt tcctcacata
     1441 attcaagcat tttttgtact ttcagccccg tatggaaaaa gttcaatttg ttattcagca
     1501 cacggctcaa agtcaacgag cagtagaaaa taccttaaaa ttattggaat ctggtgcaac
     1561 cgtacctttc attgctcgtt accgcaagga gttaactgga aatttagacg aagtgcaaat
     1621 cacctctatt cgtgatgaag ctaaaaaata cgatgaaatt gaagcacgcc agcaaaccat
     1681 tttgaaaagc attgaacaac aagataaatt aacacctgaa ttaaaggaaa aaattacaac
     1741 tacgtttgat ttagttgagt tggaagatct ttatttgccc tacaaacaac gcaaagaaac
     1801 acgtggcgat aaagcaaaaa aagcaggatt agaacccttg gcaaaaatga ttatgtcgca
     1861 acgaggcgat ttatacgcaa tggccaaccg ttttgtaaaa ggctcaatca atgatgaaga
     1921 cgaagccatt caaggagcgc aagacatcat tgccgaatgg gtaaacgaat caactgctgc
     1981 acgtgcacgc ttgcgcaacc tctacacacg caaaggaaaa ttaagttcta aactagtaaa
     2041 aggcaaggaa atagaagcgg agaaatacaa agattatttt gatttttctg aacctttgtt
     2101 taaaacagct tcgcaccgat ttttggcatt gtatcgtgct gatagagaag gtttgttgaa
     2161 aatcactatt caaccagaaa aagaggaagt tttggaaacc ttgcatcgaa tgtttgtgaa
     2221 aggcaacgac gattgctcag cagttgttga aaaggcttgt gatgacgctt acaaacgttt
     2281 gttagctcca tcgctacaaa atgaaatcct acaagcacaa aaagaaaaag ccgataagga
     2341 agctatcaaa gtgtttagca ccaatttgcg tcaattgtta cttgcgccac ctttgggcga
     2401 aaaacgagtg ttggcaatag acccagggtt tagaaccggc tgtaaagttg tgtgcattga
     2461 tgagcacgga agtttgttga ccaatgccac catttaccca catccacctc aaaaagaaac
     2521 aagtgcagcc aaagcaaaaa ttgcacaatt ggttgaagct tacaaaatag aagcaattgc
     2581 aataggtgac ggaacagcgg gacgtgaaac agaaacattt atcaaacaca ttcaattcaa
     2641 ccgtgatttg ttggtgtttt ctgtgcgcga agatggagct tctatttatt cggcaagttc
     2701 cattgctcgt aaagaatttc ctgattacga tgtaactgtg agaggagcgg tttctattgg
     2761 tcgccgattg atggacccat tggctgagtt agtaaaaatt gacccgaaat cggttggtgt
     2821 gggacaatac cagcacgagg tcaaccaaac acaattgaaa gaagcattgg acgatgtggt
     2881 tgtttcggct gtaaacacag ttggggttga cctcaacaca gcttctcctt atttgttaag
     2941 ttatgtttct ggattaggtc cacagttggc agaaaacatt gttgcacacc gtacagaggt
     3001 tgggaaattt acttccagag aagaattgaa aaaagtaaaa cgtttgggtg ataaagcgtt
     3061 tgaacaagca gcaggatttt tgcgcattcg cgaggggaaa aacccacttg acaattcatc
     3121 ggtgcaccca gaaagctaca agcacgttga aaaaattgct aaaaaattgg ggttgaaaac
     3181 cgaagaatta attggcaatg ctgaaaaact tgcaacaatt aagcaagcgg attttcaaga
     3241 aattgatgct tatacatttg ccgatattgt aaacgagttg aaaaaacctg gtcgcgaccc
     3301 tcgcaaacaa gccaaagtat tagaatttga ttcatccatt cgcaccattg aagatttaaa
     3361 aattggaatg gagttggaag gaattgttac caacgttacg gcatttggtg cttttgtcaa
     3421 tattgggatt aaagaaaatg ggttgattca caaatcgcaa ttggcagatg aatatgtgga
     3481 agatccttca aaattcatcc aattgcatga acacgtacgt gtaaaaatta ccgaaattga
     3541 tattccacgc aaacgagttg gactcaaacg catgaaatag cgattatttc acgtaatctt
     3601 gcttaacaac aagaatgtat tggtcgtttt caattttttg atagccttgc tcgtagcaaa
     3661 aaaagtaaac ggtacctttt tgtgttttgt aagtattggt aaagtagtga aaattgaatc
     3721 cttcttcggc taaatggata ccatcaacgg ttgttttgcc tttgggattg agtttttcta
     3781 aaatacggcg gtttttgcgc aaaatattat tgattcttcg cacgtatttg ttggcatctt
     3841 cattttgacg attgttgtag gtatttctac attgatcgct gcaaaatttt tggtcttttc
     3901 gccctcttaa aacggtgcca cattctaaac atttacgatt ttccatttgt aattgttatt
     3961 atttcacaat gttaacaata tttaacagca aaatcaagta tttcagcaag agatataagc
     4021 aactttgtag gttgtactga agacagtaga ttatgacaaa tgaaatgaat gattctttga
     4081 gtccggctga ggctcttaga cagttaagtg aacgtatttc catagaaatt gcaggcatca
     4141 acctgttgag agaagaaata gcaaaagtta ttgttggtca aacctacatg gttgatcgat
     4201 tattggtggc tttattggcg gatggtcacg ttttgttgga aggggtgccc ggattagcaa
     4261 aaacattggc cattaaaaca cttgccgatg ccatagatgt tgattttaac cgcattcaat
     4321 ttacacccga tttattgcca gcagatgtaa caggaacttt ggtttatagt ccaaaaaacg
     4381 aatcattttc ggtgaaaaaa ggacctattt ttgccaattt cattcttgca gatgaaatta
     4441 accgtgctcc agcaaaagta caatcggcct tattggaagc catgcaagag cgtcaagtta
     4501 ccataggcga agaaacattt gcacttccac aaccattttt ggtgttagca actcaaaacc
     4561 caatagaaca agaaggaacc tatccgttgc cagaggcgca agtggatcgt tttatgctca
     4621 aagtgtttat ggattatcca agcatggaag aagagcgcat gattatccgt tccattgttc
     4681 gccccgaagg tttgccacaa gcaaaacaag tcattaaagc aagtgatatt gtgcgtgcac
     4741 gctcgttggt gaaggaaatt tacatggacg aaaaaattga aaaatacatt gttgatttgg
     4801 tgtttgctac acgtcgccca agtcaatttg gattggagtt tttagaacca atgattgcat
     4861 atggttgttc gcctcgtgct tctatcaatt tggcattggc agctaaagcg tatgcctttt
     4921 tgcaaggtag agcatttgtt attccagaag atattcgtgc cattgcacca gatgtgatgc
     4981 gccatcgtat tgggttaact tacgaagcgg aggccgaaaa tttaactgct gcacaaattg
     5041 tttcaaaaat cattagtaaa gtagaagttc catagatatt ttagccatgg aagcgcaaga
     5101 gttatttcag aaaatacgtc gtttggagct caaagcaaaa gggctcacca aacatatttt
     5161 ttcgggagaa taccattcgg cattcaaagg aagaggtatg gcattttctg aagtacgcaa
     5221 ctaccaagtt ggagatgaga taagaaccat tgattggaat gttactgctc gattcaatga
     5281 tccgtttgtg aaagtttttg aagaagaacg cgaattgacg gtaatgttgg tggttgatgt
     5341 ttccaattcg gcagattttg gctctactgc cacatcgaaa aaagaaatgt tgctagaaac
     5401 catggcaatt ttagcatttt cggcgctgtc caacaacgat aaagtgggag ttatttttgt
     5461 gagcgataca gttgaacgct acctaccacc caaaaaagga cgtcaacaca ttttgtattt
     5521 gctgcgcgag ttcattgcat ttaaagcaaa aaacaaaggc acgaacctca acaatggctt
     5581 gaaattctta aaaaatacac agaaaaaacg caccatttgc tttgttataa gcgattttat
     5641 agatgaaagc aattacatgg aaggattgaa aattgcccta aaaaatcacg atgttattgc
     5701 cttgatgttg gaagatcctt ctgaatatga attgccaaaa atgggatttt tacagcttta
     5761 caatgccgaa acgaatgcca caacttgggt agattccaat aatccgcaag ttagagccac
     5821 ctacaaagcc aattttgagg ccaataagca gaaaattaca aaggaattta cagccaacgg
     5881 aattgatttt gccgttttaa atacaggaag tgattatttt ttagagttga taaaactatt
     5941 caaaaataga tagatgaatg ggttgaaaaa taagacgatt gttcgtgttt tgatgtttgt
     6001 tttacttaca tcaacgctat caatgtgtgc ggtttttggg caggagttaa aagcaactgt
     6061 aaacaaagac aaagtacagt tgggcgaacc gtttgaattg catttaaccg ctcgtttagc
     6121 accaaattca acgataaaat ttgtaccagc agttagcgaa ttagcagcct tacaaaacga
     6181 tgaaaaagat attgctttgg aagtaacaac tgctttttcg gatagttcta attccactca
     6241 agaggcaacc atttggactg gaaaatatac cctcattgct tgggaaacag gaaaaattac
     6301 cattcctatt caaaaaataa gcatcaacga ttcaacttat ttattcaatg ccacaacggt
     6361 ttacgtcaac ccacaaacca ttggtgcaaa aaacgaattg tttgacattc aagaaggttt
     6421 ttccaacgtt tctactgatt atacattatt ggaattgctt caaaaatatt ggtatttcgt
     6481 tgctattgca ttggcagcaa ttgcagggct tttatggtgg tggcgcaagc gaaaacaaaa
     6541 aaccgtacag cctattgtcg ttttaccact tgatgtgcgt gctttaaatg ctattgatgc
     6601 gttggacaac aaaaaattgt ggaccaaagg acaattgaaa caacattaca ccgaattatc
     6661 gcatattttg cgcagttata tgtcggggcg ataccaatta aacttgttgg aaaccaccac
     6721 atttgaaacc aaagcattgt taaaacaagt tggcgtaaat gaagcaacca tttcctctta
     6781 tttgctatta ttgaaacaag ccgatatggt gaaatttgca caatcctctt cggatgaaac
     6841 aacattgata aacaactcgc gcttagcaaa acaattgata acctccacta ccccaactga
     6901 tataaaatga caatgccctt caacatacgc atttgggaat atgatttctt tgctccacat
     6961 tggttgtggc tgttgttgtt attgcctttg ttgtattggt atttgcatca aaaggagcga
     7021 aaaaaacaag gattttggaa gtttacagga acggtttcca atcagcaaca attgcgctcc
     7081 aactacgttg aatgggtgca aaaatccatc ctactaattt tttgtttggt ggttggattg
     7141 ttcattattg cattggcaaa accctacaac tggaactctg ctgatgaaag caacaacaat
     7201 taccaacatg gaattgacat tattttagca ctcgacattt ctggatcgat gttggaacaa
     7261 gatttttatc ccaaccgcat ggaagttgct aaaaaaatag ccaaagaatt tgtggacggg
     7321 cgcaaaggcg acagaattgg gttggtagca tttgctggag cggcatacac gtcttgtcct
     7381 gcaacaaccg actacgatgt gttgaaaaaa caaattactc agttaaattg ttatgccaat
     7441 attgagagtg gaaccgccat tggtgttggg ttgggaacgg ccgttactcg attaagaaac
     7501 gatagtattc cttctaaagt aatcattttg ctaaccgatg gaagcaacaa cgcaggggca
     7561 attagtccgc aagaagcagc cgaattggct aaagcgaaaa acattcgtgt gtacaccata
     7621 ggcgtgggtt ctgaagatgg catgattaaa attgaaacgc catttggaac cattcaacaa
     7681 ccggtagaat cggatttgga cgaagcaaca ttgcaacaaa ttgcatccat taccaatgcg
     7741 cattatttta gagcaaccga tgaagaaagt ttgcgtgcaa tatacaagga aattgaaaaa
     7801 atggaacaac gaaaaattat cgaccattat tttcaagccg aaccgccagc tatgcccaaa
     7861 aatttcttaa ttgccgcatt gatattagcc cttgttggtt ggagcgtaca acaattgtta
     7921 tttaaacacc atgaataaac catcaacaca tacactaaaa aagtttacct tgctaatttt
     7981 agcttgggaa gttgcttttt ggttgctttt tggcggattg tttcttgttt taaatactcc
     8041 aaaagggatg ggaaacagct atttttcgtt tgagaatcct gagcaattgt ggtggctatg
     8101 gctgttggga cccattatcg gattgttttt atatacaact tatcaatctc agcatacatt
     8161 ggcggtgatg ggtaaaaaaa cacgtttaac catcgtaaaa acagcatcgg ggagcaaaca
     8221 attgttgcgt tttttccttt ttagaaatgc ggtggtattt ttaatcattg ccttggcgca
     8281 acctgctttt gggacaaaga aaacagctgc caccattaaa agtatggaat tggtggtgtg
     8341 tttagacatt tccaactcca tgaatgcgaa cgacattgac cattctgctt cgcgattaga
     8401 catagccaaa agagccatca tggagttaat caacggactt tcgggagaaa aaattggaat
     8461 taccattttt gccaatgaag catttaccca attaccgttg accttggatt atcaagcggc
     8521 taaaatgttt gtgcaagaaa tagaaaccaa catgataagc aaccagggaa ccaacattca
     8581 aaaagctttg gaaaatacgt atgcattctt ctccaaaaac aaaaaattga gcaaagccgt
     8641 gttgttgatt accgatggcg aaagtcaaga gggcaaccca tcaacaatat tggaacgcta
     8701 caacgaagat ggaattcatt tagcggtatt gggaataggc acacaaagtg gtggactcgt
     8761 tccaattgac cccgagcgac ctgaattggg atacaaacgt tcagcgcaag gagttgccgt
     8821 tcaatcaaaa ttaaacccag cgatgataca gcaaattgcc gctgaggcaa atggaattgc
     8881 actcattagt gaaaatgcat atccgaattt aaaaggttta ttaaacgata ttgccaatat
     8941 ggaacgagca agcacaacag gaatggattt tgaggttaag aaacaacgct atcaaattcc
     9001 gttgctcatt gcccttgctt gttggttgag ttatttgctt ttacctattg tttcactaaa
     9061 aaaacacgtg tcatgaagtt ggtaatgttg agtgcattta tggtgttaac atcagttgtt
     9121 tttggtcagc aatggaaaga ctcacttgct gctgcccgaa aagcgtatga gcaaaaaaac
     9181 tacagcaaag cattgaaatt gtatcaatcg gcacaaaaaa atgcaccaaa agacattgat
     9241 ttatccaaag aaattgggca agcaacctat aaatcatcca actattcgga tgctgaaacc
     9301 ttatttcgcc aacaagcatc aaccgaaaaa tcggcaatcg aaaaagcaaa agcctaccat
     9361 aattggggta atagtttgat acaacaaaaa aaatacggcg aagccattga agcctataaa
     9421 caatcgttgc gcaacgatgc tagcaataac aaaacacgcc ataacttatc cgaagcgatg
     9481 cgtttaagaa aagaagaaga acaaaaacaa gacaatcaaa ataatcagaa ccaaaataac
     9541 cagaatcaaa accagaacaa tcagaaccaa aaccaaaata acaataaaca acagcaaaac
     9601 aataaacaaa accaaacaaa caactctgaa aacaaccacc aaaaggacaa ttccaacaac
     9661 tcgcaattgc aggataaagc cattgaaaaa aaattagacg aattaatgaa acaagatgcg
     9721 tccactaaac gtaagttagg gcaaggaaaa aacccaagtg gcgcaccaaa atcaggaaat
     9781 gattggtaat gaaagcagta gtagtacata tttgtttttt gttttgttct ctttttgttt
     9841 ggggacaaaa agcaagtgtt aaattattgg ttgaaccaca agaagtgagt gttaatcagc
     9901 aattttccat tacattgaaa tccaatgttg atgggaacat tgtggagaat tggccctcca
     9961 attttgaacg tggttatggt gtgcaatcca tgtcgcgcta tgtgcaagat tacagcaacg
    10021 gaaaaatggt gcaagaacac cttgtcattt ttacaggcga atttacaaaa gcgggtagct
    10081 ataaaattgg tccgttttat gtcaaagcgg gcaacaaaac ctacacttcc aattcggtta
    10141 tggtaacggt tggtggtgca tccaacaatt ctaatggtgg agaaatcaca aaaaaacagc
    10201 tcaatcaacc ggcatttgga gtgatagaag tatcggcaac aaaattgtat gaaggcgaac
    10261 ctttggtggt ttcgggtcgt gtgtacgcgc gcgaagaaac ttttggacga cctattttaa
    10321 aacgcccttt caattttaac ggcgttagtg atatgcacga gttgcaacaa actgaaatgt
    10381 gggaacccaa agtggtgcgc aaccgcgaat acctttcttt ttcttttggc aaacaagtat
    10441 tgtttcctgt tggtagtgga cgtttagaca tccagtcatt tgaaatattt ttaccatttt
    10501 ttgatcaaaa ttacaatgtg gtgtcctcta ctccttcggt tgaaattatt ccattgccaa
    10561 gcaatccgcc tgctaatttc tgtggtggag tgggcgaatt ttcggtggag caaaaaattg
    10621 taaaccctca aaacgttaaa caaggcgata tagttcaaat agaagttact gttaacggaa
    10681 aaggaaacct tcacgccatt gaaagtcctg tgttacaatt gcctaaagga atgaatattt
    10741 atggcgatgc cgaaacgaag gatgattttt cgtttacttc gcaaggatca actggtaaaa
    10801 aaacatatac ctatcatgtg caagttaccc aagaaggaaa acaagttatt gagcctgtag
    10861 ctattgccta tttcaacccg aaaacagaaa aatacgcaac agcatctgct gcaaaatcaa
    10921 taacggtgga tgtgctagtt aacaaagcgt ttagtttgca gacagaaaac accgcaacac
    10981 cgcaacccgt caacgaattt gataaaaaat acaattattc caaattgaaa agtgttctca
    11041 cctcatccaa cagttggttg tggtatggtg caggtagttt actccttctt gctggattgt
    11101 tgtttatatt ttaccctcgc aagaaaaaag tagttgctcc aacggttgaa atcactccta
    11161 ccattcaaat tgaaacaacc agcgcaaccg aagtgcaaga gcaaattgct cgcttagaat
    11221 tttacgctaa agaacaagca cacgatttgt tttacgcagc tcttgaaaaa acgcttttaa
    11281 tggtcttaaa ctttcacttg caaatagata aaaacaatca actttccaga aacgaattgt
    11341 tgagtgcttt gcgcaacaaa caacccgaaa aagcaataca aatacaacgt ttattcgaaa
    11401 aatcagacgt ttctcgctat ggaatgggct ttaatgcgga ggaaatggag agtgtggtgc
    11461 aggaagtgaa ggggatagca taaaaaagga gtgttgctaa ttggttaaca cactcccttg
    11521 cttcgttttt aatttttata ggctcaatct gccataaacc ttcttcaaca aataatccat
    11581 caccaaacca ttgaagtctt ggtcgttcat catacgggta aaaatgtctt gattttggtc
    11641 cattctacta ataaacttat ctattgctat ttctttgaaa ccgtatttaa aattttcgat
    11701 ggaatttgat tttgcttgtg ctgccaactg tttgtctgca accatatcct cttcaatttg
    11761 gtcaaataat aatttatctg ctacagtaaa ttctgttcca aatcgcttgt tgatgactgt
    11821 aataatttcg gataaagctg ctttttcatc tttagtcatg cgtattccag cattattaat
    11881 accttctaaa ccgtattcac cttttggttc taacaagatt tgctgttctg caactttttg
    11941 gaggcgataa tattccaatg aaacttcatc attcagttga aagcgttcac tttggttttt
    12001 cttaggtagt tttttatgta aataacggct atatgcatac aatttttcca actcaacatc
    12061 agaaaaaggc attatttgag ttaaaaaaga ataaatcctt acaaatgatt gaagagcatg
    12121 tttgaaattt tcttgcgaaa catcaactac tacatctcct tgagtggttt cttctggtaa
    12181 ctttttataa cgatctacag ctgggtcaat ataactattg agtttgcctt ggtcgttttt
    12241 agaaagaatt tgaacatctt tgaaatacac attgcaaaaa ttatctactt ctgatttcca
    12301 aattacttgt gctttttcta tttctgtttt taagtcgtat aatcgattag ggtctgttgt
    12361 ttcttgaaca gtggttaatt cataatacgg ctgaaaagat tctagaattg catctgcttc
    12421 attaacaaaa tccaatataa aagtatcgtc ttttcctgca catgttctat tgagtcgcga
    12481 caatgtttgc acacatttta caccagacaa cttcttatcc acatacatag tatgcaacaa
    12541 aggttggtca aatcctgttt ggtatttatc ggcaaccaac aacaattggt attcatctgt
    12601 tgcaaaacgt ttgggaagtt ctttttcttt gaacccattt aattcggctt cggtaacacc
    12661 ttctggataa gtgtcataca ttacttttcc agaaaaagca accaatgttt taatatcaga
    12721 atagtgatgc tcggctatgt atttatcaaa ttcttctttg taacgtactg catgaagacg
    12781 agaagatgta acaaccattg ctttagcttg tccacctatc tttttggcaa ctacatttcg
    12841 aaaatgctcc accatcactt cggttttttg cgataaatta tgtggatgca gtgacataaa
    12901 gcgacctatg gcacgagatg ctgctttttt atttacattt ggatcgtttt caatatcttt
    12961 ggtgagtttg taatatgttt tataggtggt ataatgtttt aatacatcca aaataaaacc
    13021 ttcctcaatt gcttgtttca tagaatacaa gtgaaatggc tttggaattc cgtttttatc
    13081 tggcgttcca aacacttcaa ctgttttatt ttttggtgta gcagtaaacg caaagaaact
    13141 taaattctgt tgttgtccac gtgcttccat ggattttcgg atttggtctt ctgcatcttc
    13201 atccaaacca gaatcaagtt cttgtgctgc cgcctcttcc agagattttg ccgaaagcac
    13261 ttctttcatc tttttacttg cttcgcctcc ttgtgagctg tgagcttcgt caataattac
    13321 cgcatatttt cgtgcctcca acgcaccaat tttatccaaa ataaatggaa atttctgaag
    13381 cgtagtgatg atgatgtttg aaccagtaga aatggcattt gccaattgtt gagaatcttt
    13441 atctattttt tgcacaactc cctctttgtg ttcaaactga taaattgtgt tttgcaactg
    13501 gctatctagc acttttcggt ctgtgattac aattactgaa tcaaaaatgc gctcgttttg
    13561 gtcattgtgc aaactggaaa ggcgatagga aagccatgca atagaatttg attttccaga
    13621 gcctgcggaa tgctgaataa ggtagttttt tcctgcacca tgttttcgtg catcttgtgt
    13681 taattcacgc actacttgca attggtgcaa acgagggaaa attaatgttt cttttatttt
    13741 cttaactccg ccaattttta tttcttcaac acttaggtgc ataaatcggc cgatgatgtc
    13801 cataaaacta tcctttgccc aaatggtttc ccacagatag gcagtgcgat agccattttg
    13861 attgagagga ttgcctgcac cattttggtc gcctaaatta aaaggcaaat aacgtgtatt
    13921 tgtgcctgcc aattttgtgg tcatgtaaca ttcgtcggta tcaacagcaa agtgtaccaa
    13981 agctcgcttt ttaaactgaa aaattggctc tgtttgatcg cggtcaaacg catattgttt
    14041 ttgtgcattg cttacatttt gaccagaaaa ttgatttttc aactcaaccg tagcaatagg
    14101 aagtccg
//
LOCUS       DAOQVQ010000009        13621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5092_length_13621_cov_3.683400, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000009 DAOQVQ010000000
VERSION     DAOQVQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_13621_cov_3.683400"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PKN22_00670"
     CDS             <1..1341
                     /locus_tag="PKN22_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41243.1"
                     /translation="LIVLLLLNFFCSTTFSQCLVGSLSGTITAPTSGSWTGVSATYNQ
                     HTIVNGLVAGETYEIKNNVGNRICVYDASNNLVLYSNDSHVSFVAPTGVSSIKVVICN
                     NGVNNGNFQIKCSYCGTSDPTGTGNCIATNNSNTTLTIDNPGWLGQQYFSAPSFNTYI
                     RLKNLVKDESYTIFQGHCGTNERNYVLVRMGNSPSGAVVAQGYLPLSFVSPETSDNSG
                     NNSGGPGTGPYQFYLHIFKSHACDGTNFEQMCGQRFSFGPTIPLSVIVSSFTGEPTNN
                     GNLLEWSTTTEINNDFFEVEATEDGEHYRTVGKLIGSGIGTFRETKSYSLLDENATFG
                     TTIYRLKQVDVDGKVSYPSTIAVNRKGTNDITIAPNPFKNELKINLNVDEQGAYNFNF
                     VDALGKNYTIQKDLFKGYNQLNVDLSTTLQSGFYILKITNLEGEIINTTKIIKD"
     gene            1506..2654
                     /locus_tag="PKN22_00675"
     CDS             1506..2654
                     /locus_tag="PKN22_00675"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41244.1"
                     /translation="MKYTVILILNFLALGFISNLYAQVPAFGVDSLLDVGCWNIEWFG
                     DPDKGPSNEQVQYNNVKQVLNNTDIDIWGLTEMSNPTSYQQLLNDLPQYGGCLATYDQ
                     TQKTSVFYKKDMFEVIQSHHILSSSQYNYDFAGRPPFEVVLKTKSFDNPDTIYVIVVH
                     LKAFADQESYNRRKDAINSLKSVFLDVQRKDKKTIVLGDWNDDVDVSTYNGIQTPFQQ
                     LLDDPQNYFFPTKALSDAGKTTYASYSGSFIDHILITNPLKIDYKPASAKVLDMMPTY
                     ISSYTSKTSDHYPVIASFHLDNINTLDVDSIVEETLSFAVLMDNQSLLVQGNWMGNVE
                     IIGVDGTKLNTVEKQTEHLQIPINELPQGMYIATMRLQSGIKTFKFIR"
     gene            complement(2712..4256)
                     /locus_tag="PKN22_00680"
     CDS             complement(2712..4256)
                     /locus_tag="PKN22_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNS41245.1"
                     /translation="MQTKILWADDEIELLKPHILFLESKGYAVTAVNSGLDAVEKSAE
                     EDFDVIFLDENMPGITGLEALSQIKEQKSHVPVVMITKSEEEYIMEEAIGSKIADYLI
                     KPVNPNQILLSLKKILEQTKLVSQKTSSNYQQEFRQLGMQLGGRLDANEWAELYKKIV
                     YWELELQQTKDASMMEILAMQKREANTLFAKFIERNYIDWLHGDADAPQMIHTLFNKR
                     IAPIVDKEKTYVLVIDNLRYDQWKILQPVISSYFTVESDDIVYSILPTATHYARNAFF
                     AGLMPLEIERKFPNLWVSEEDEGGKNMHEDAFLEANLKRLGKSYKWSYNKITNLAAGK
                     KLVDNFNQLKDNQLNVIVYNFVDMLSHARTEMEVIKELADNEAAYRSLTLSWFEHSPL
                     QDMVKKIADQKARLVITTDHGTMKVTNPVKVIGERATNTNLRYKTGRNLSYNEKEVFR
                     ISKPEEAFLPKGSLSSEFIFTKEADFFIYQNNYNKFVNFYANTFQHGGISLEELLIPY
                     IVLKAK"
     gene            complement(4332..5021)
                     /locus_tag="PKN22_00685"
     CDS             complement(4332..5021)
                     /locus_tag="PKN22_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41246.1"
                     /translation="MVRFIKYTFLFSFMLILGQATAQFSVGGGATTMMEFGNNKPFYG
                     LHFNMEFPRNNQVTFYGRLTYLFNQNKKEYISDLTATAIDASTDPQWVTVPMNNVSSI
                     NYFMIDGGTRYYLINGFDEGFSVYGGTNLALIVNSVKHGYQFGEYDQSKYLLNAGDFS
                     DARQKGTIMRLAVGFTGGIKYTFPGKGTIYFDFNPQLTLFGLPSNQNIPSSVYKPVFF
                     GFNIGYRKEIY"
     gene            complement(5006..5548)
                     /locus_tag="PKN22_00690"
     CDS             complement(5006..5548)
                     /locus_tag="PKN22_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNS41247.1"
                     /translation="MSYCSYCENLPLDNLHRIYHDTKYGFPIESDNELFGRLILEINQ
                     AGLSWDTMLKKEQAFRKAFEEFDIQKIANYQDKERERLLNDAGIIRNRLKIDAVIYNA
                     GAILALQKQYGSFKNWLDMQEFTTKEEWLKEFKKHFKFVGGEIVNEFLMSSGYLKGAH
                     NEKCIIFDKIIKSKPRWLDL"
     gene            complement(5615..8152)
                     /locus_tag="PKN22_00695"
     CDS             complement(5615..8152)
                     /locus_tag="PKN22_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNS41248.1"
                     /translation="MEAKFSPRVKDVIGYSREEALRLGNDFIGVEHLLLGLIREGDGT
                     AIKIMTEFHVDLRQIRNEVEVTLLKSSSKAYTNSGQIPLTKQAEKVLKRTYLESKVFK
                     SSMIGTEHLLLSILKDEESVSCRVLNKYGILYENVKDELNMSSQNPDTPFSTSSMPRA
                     EFPGAGGEEEDQDFGAQRKPTDPKSKTPVLDNFGRDLTRMAESGRLDPIVGRENEIER
                     VSQILSRRKKNNPILIGEPGVGKSAIAEGLALRIVQRKVSRVLFNKRVISLDLTSLVA
                     GTKYRGQFEERMKAVMQEIERNPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARG
                     EMQVIGATTLDEYRQYIEKDGALERRFQKVIIEPTSVDETIQILNNIKERYEEHHLVS
                     YTPEAIVACAKLTERYITDRFLPDKAIDALDEVGSRVHITNINVPQEILDIEQQIENL
                     KDEKNAVIKSQQYEKAAELRDTERKLQDQLETAKQKWEAESKNNRVTITEDHVAEVVS
                     MMSGVPVTRISESETKKLANMALELKGSVIGQDDAVAKVVKAIQRNRAGLKDPNKPIG
                     SFFFLGPTGVGKTQLAKVLAKYLFDSTEALIRIDMSEYMEKFSISRLVGAPPGYVGYE
                     EGGQLTEKVRRKPYSIVLLDEIEKAHPDVFNLLLQALDDGHMTDGLGRKIDFKNTILI
                     MTSNIGARQLADFGTGVGFGTKAQAESRDENAQTVIQNALRKAFSPEFLNRIDDIIMF
                     NSLKREDIFKIIDLELKKLYQRLDNLGYHIELTEAAKDFIVEKGYDEKFGARPLKRAI
                     QKFVEDPLAEEIINSELKEGDTLKMDLNETKDGLITIKDKGKTKQKA"
     gene            8381..10939
                     /gene="gyrA"
                     /locus_tag="PKN22_00700"
     CDS             8381..10939
                     /gene="gyrA"
                     /locus_tag="PKN22_00700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNS41249.1"
                     /translation="MADGERIVKINIENMMKSAYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLYGMQELGVYSNRPHKKSARIVGEVMGKYHPHGDASVYDTMVRMAQHWSLRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLDKIAEDMLDDLEKETVDFAPNFDESLSEPTVLPT
                     KIPNLLVNGASGIAVGMATNMAPHNLSEVVDGTIAYIDNNDITGEELLQYIKAPDFPT
                     GGIIYGYEGVKDALLTGKGRIVMRAKAEIESTGSHEQIIVTEIPYQVNKAEMIKRTAD
                     LVNDKKIEGISDIRDESDRNGMRIVYELKRDAIPNVVLNKLFKYTALQTSFSVNNICL
                     VDGRPQLLNTKEMLMHFVDFRHDVIYRRTQYDLRKAEERAHILEGLIIASDNIDEVIK
                     IIRGAESPQAAIDQLMARFSLSEIQAKAIVDMRLRQLTGLEQDKLRAEYADLMKLIED
                     LKDILANKSRIMQIIKDELTYVKEKYGDDRRSEIEYAASEMRIEDLIPDEEVVVTISH
                     AGYIKRTSLSEYKVQNRGGMGSKGSATRDKDFLEELFVATNHNYLLVFTEKGKCFWMR
                     VFEIPEGNKTSKGRAVQNLINIEQDDKIKAYVKVQDLTDSEYVDNNFIIMCTKNGVIK
                     KTSLEAYSRPRANGINAITVRDGDELLEAKLTNGTNEIVLATREGRAIRFNEQTVRPM
                     GRNASGVRGVTLTSKTDAVIGMICVEDVYSTVMVVSEKGYGKRTFLNDPEDQEPIYRI
                     TNRGGKGVKTLNITDKTGALLSIKNVTDEEDLMIITKAGTTIRMHIDSIRTMGRAAQG
                     VRLINLKGNAEIAAVARVPRSDEEDEEGEFDETNTPLNDAPEENGTTIEDGNEE"
     gene            10954..12159
                     /locus_tag="PKN22_00705"
     CDS             10954..12159
                     /locus_tag="PKN22_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41250.1"
                     /translation="MKTNKITVLAGCLMVSALSFGQKTNVTSAAVEYKQKFQPSLMMG
                     KVDDAKQALLNAKKYIDLAAEHPDTKADPKTLYYKGEIYGGAVQLASMTADTNFMINN
                     FGKDAMEVSIKSLQESYKMSSKFRPDIDQSVNMQVGMLSPMASKFYEEGKYKEAGAAF
                     YFIYKVTTAKNEFDTTNLYNAAVCLEKADMIKEAAETYSELAEIGYKKGEGYAFAANM
                     YTKLKDYDKAKEILLKGKAKYGNDKAILLELVRVDMAKGDNAAAEKSLSDAIAADPKN
                     KQLYYIIGTIYTELGENEKAEKALLSALEIDPNYLDAQYNLGAHYVTWATQLRDKANT
                     LNENDFNYDMLLSQSRDLYKKAIAPLEKYIVSQPKDANVLLILFQINQNLGNSDKAKE
                     YKARYDAAK"
     gene            12265..12648
                     /locus_tag="PKN22_00710"
     CDS             12265..12648
                     /locus_tag="PKN22_00710"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNS41251.1"
                     /translation="MITEEKIKASETRIFKAVFPNTTNHYDTLFGGTAMQLMDEVAFI
                     TATRFSRQRMVTVSSDRIDFKKPIPAGTIIELVGKVTYLGSTSLKIRVEIYIEQMYEE
                     HREKAISGEFTFVAIDESKKPVKIQ"
     gene            12718..>13621
                     /gene="gcvT"
                     /locus_tag="PKN22_00715"
     CDS             12718..>13621
                     /gene="gcvT"
                     /locus_tag="PKN22_00715"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNS41252.1"
                     /translation="MKQITLNDIHVALGAKMVDFAGFYMPVQYEGVNVEHETVRNGVG
                     VFDVSHMGEFLISGKNALALIQKVVSNDASTLTDGRAQYAYLPNENGGIVDDLIIYRF
                     NEEKYLLVVNASNIDKDWNWISKFNQEIGAEMRNLSDDYSLLAIQGPKAAEAMQSLTS
                     VNLSAIPYYHFEVGYFAGFEDIIISATGYTGSGGFEIYCKNDQVKQIWDKVFEAGASF
                     GIKPIGLAARDTLRLEMGFCLYGNDIDDTTSPYEAGLGWVTKFTKDFVNADFLKAQKE
                     NGVSRKLVAFELIDRGIPRHDYEIT"
BASE COUNT         4439 a         2433 c         2459 g         4290 t
ORIGIN      
        1 ctaattgttc tgctattact aaatttcttt tgttcaacta cttttagcca atgtttagta
       61 ggttcattga gtggaacaat aacagctcca acatctggtt catggacagg tgtctcagca
      121 acatacaatc agcacactat tgttaatggc ttggttgcag gtgaaacgta tgaaattaaa
      181 aacaatgttg gtaatcgtat ctgtgtttat gatgctagta ataacttagt gttgtattct
      241 aatgactctc atgtttcgtt tgtagctcca acaggtgtaa gttctattaa agttgtaatt
      301 tgtaataatg gagttaacaa tggaaatttt caaataaaat gttcttattg tggaacatct
      361 gatccaactg gtacgggtaa ttgcattgcg acaaataatt ctaatacaac tttaacaatt
      421 gataaccctg gttggttagg tcaacaatat ttttcggcac cttcttttaa tacctatata
      481 cggttaaaaa atttagttaa ggatgaatcg tatacaattt ttcaaggtca ttgtggcaca
      541 aatgaaagga actatgtttt agtaagaatg ggaaacagcc cttctggggc ggttgtggct
      601 caaggttatt taccgcttag ttttgtttct cctgaaactt ctgataattc tggaaataat
      661 tcgggtggtc caggcacagg cccttatcaa ttttatttac atatattcaa aagccatgca
      721 tgtgatggaa ctaactttga acaaatgtgt ggtcaacgtt tttcctttgg tccaacaatt
      781 cctctctcag ttattgtctc cagttttact ggtgaaccaa ccaacaacgg aaatttatta
      841 gaatggtcaa caacaacaga aattaacaac gatttttttg aagtagaagc aaccgaagat
      901 ggagaacact atagaacagt gggtaaacta atcggttctg gaataggaac gtttagagaa
      961 acaaaaagct attcattatt ggatgaaaat gcaacttttg gaactaccat ctatcgcctt
     1021 aaacaagtgg atgtggatgg aaaagtgtct tatccttcta ccattgcagt aaatagaaaa
     1081 ggaacaaacg atataacaat tgcacccaat ccatttaaaa atgaattaaa aataaactta
     1141 aatgtggacg aacaaggagc ctacaacttt aattttgtag atgccttggg aaaaaattac
     1201 acaatccaaa aggatttatt caaaggatac aaccaattga atgttgattt gtcaaccaca
     1261 ctccaatcgg gcttctacat ccttaaaatt accaatctag agggtgagat tattaatacc
     1321 accaagataa taaaagatta accacttaaa ataaaaatga taaaaggcgt tcaaaaattg
     1381 gacgcctttt gtgttttgga gaatatgtca atatgcagat agttcgatat gtcgatattc
     1441 atagttcgat atgtcaatat tcaaatagtt cgatattact aacagtattt tgtatttttg
     1501 caattatgaa atacaccgtt atactcattt taaatttttt agcacttgga tttatttcca
     1561 atctgtacgc gcaagttcca gcttttggag ttgattctct tcttgatgtc ggttgttgga
     1621 acattgaatg gtttggtgat cccgataaag gaccttccaa cgaacaagtt cagtacaata
     1681 acgtaaaaca agtgttgaac aatacagata ttgatatttg gggactaaca gaaatgtcta
     1741 atccaacttc ataccagcaa ttgctcaacg atttaccaca atacggtgga tgccttgcaa
     1801 cgtatgatca aactcaaaaa acttcggtgt tctataaaaa agatatgttc gaagttattc
     1861 aaagtcatca tattctatca tcttctcagt ataattacga ttttgctggt cgaccgccat
     1921 ttgaggtggt actaaaaaca aaatcgtttg ataatcccga tacgatttac gtgattgtgg
     1981 ttcatctaaa agcatttgct gatcaagaat cgtataaccg aagaaaagat gcaatcaata
     2041 gccttaaatc tgtttttcta gatgtgcaac gaaaagataa aaaaacaatt gttttaggag
     2101 attggaacga tgatgtggat gtttcaacct acaacggaat tcaaacgcct tttcaacaat
     2161 tattagatga ccctcaaaac tatttctttc caacaaaagc cttgagcgat gcaggaaaaa
     2221 caacctacgc ttcctattcg gggtcattta tcgaccatat tcttattaca aatcctctta
     2281 aaatagatta taaacccgca tcggcaaaag tgctcgatat gatgccaact tacatcagta
     2341 gttataccag caagacatca gatcattacc cagtaattgc atcgtttcat ttggataata
     2401 tcaatacgtt ggatgttgat tccatagtag aagaaacgct atcatttgct gttttaatgg
     2461 ataaccaatc cttgttggtg caaggaaatt ggatgggaaa tgttgaaata attggagttg
     2521 atggcaccaa attgaatact gttgaaaaac aaaccgaaca tttacaaatt ccaattaacg
     2581 aattaccaca aggaatgtac attgccacta tgcgtcttca atcaggaatc aaaacattta
     2641 agtttatccg ataaaagaat tttaaataaa acaaaaggct tattgaaccg ttcaataagc
     2701 ctttttttat gctattttgc ttttaaaaca atgtatggaa tcaatagttc ttccagcgaa
     2761 atacccccat gttgaaacgt attggcatag aagttaacga atttattgta gttgttttga
     2821 taaatgaaaa aatcagcttc ttttgtaaaa ataaactctg aggaaagcga gccttttggt
     2881 aaaaaagctt cttctggctt tgaaattcta aaaacttctt tttcgttata cgataaattt
     2941 cgtcccgttt tgtaacgcaa gttggtattg gtggcgcgtt cacctatcac ttttaccggg
     3001 ttggtaactt tcatagtccc atggtcggtg gtgatgacca atcgtgcctt ttgatcggca
     3061 atttttttca ccatatcttg caaaggagaa tgctcaaacc acgacaatgt taatgaacga
     3121 tatgcagctt cattatctgc caattctttg ataacttcca tctctgttct ggcgtgcgac
     3181 aacatgtcca caaaattgta aacaattaca ttaagctgat tgtccttcaa ttggttaaaa
     3241 ttatctacca acttcttacc ggctgccaaa ttggtaattt tattgtaact ccatttataa
     3301 ctcttgccca atcgttttaa attggcttct aaaaaagcat cctcgtgcat gttttttcca
     3361 ccctcatctt cttcagatac ccataaattg ggaaacttac gttcaatttc caacggcatc
     3421 aaaccggcaa aaaatgcatt gcgagcatag tgcgtagctg ttggcaagat ggaatacaca
     3481 atatcatcac tttctaccgt aaaataagag gaaataacag gttgcaaaat cttccattgg
     3541 tcatatcgca aattgtcgat taccaaaaca taggtttttt ctttgtcaac tatgggggca
     3601 attcttttat taaataaagt atgaatcatt tgtggggcat ccgcatcacc gtgcaaccaa
     3661 tcaatgtaat tgcgttcaat aaatttggca aataatgtat tggcttctct tttctgcata
     3721 gccaaaattt ccatcatact ggcatctttt gtttgctgca attccaactc ccagtaaaca
     3781 atttttttat acaattcagc ccattcgttg gcatctaaac gtccaccaag ttgcattccc
     3841 aattgacgaa actcttgttg gtaattcgac gatgtttttt gagaaaccaa ttttgtttgc
     3901 tccaaaatct ttttcaaact caacaaaatt tgattgggat taacgggctt gatgaggtaa
     3961 tcggcaattt tactaccaat cgcttcttcc atgatgtatt cttcctcact tttggttatc
     4021 atcaccaccg gaacgtgtga tttttgctct ttaatttgac ttaaagcttc caaccccgtg
     4081 atgcccggca tgttttcatc taaaaaaata acgtcaaaat cttcctcggc acttttttca
     4141 accgcatcca atccactgtt taccgctgtt acagcatagc cttttgattc taaaaataaa
     4201 atgtgtggtt tcaataactc aatttcgtcg tctgcccaaa gaatttttgt ttgcataatc
     4261 ttgttttttt agaaaacgaa ggcgtaacag taaaattgct acgcctccag tttaaaatat
     4321 gttaatctat cttaataaat ttcttttctg tagccaatgt taaaaccaaa aaacaccggt
     4381 ttataaaccg aacttggaat gttttgatta cttggtaatc caaataatgt taactgcgga
     4441 ttgaaatcaa aataaatggt tcctttacct ggaaaagtgt atttaatccc accagtaaat
     4501 cccactgcta atcgcatgat tgttcctttt tggcgagcat cactaaaatc tccagcattc
     4561 aataaatatt tcgattggtc gtattcacca aattgatatc catgtttaac agaattaaca
     4621 atcaaagcca aattggttcc tccataaaca gaaaatcctt catcaaaccc gttgattaaa
     4681 taataacgtg ttccaccatc aatcataaaa tagttaatgg aagaaacatt gttcattgga
     4741 actgtaaccc attgcggatc ggtgcttgca tcaattgctg ttgcagtcaa atcactaatg
     4801 tattcttttt tgttttgatt gaacaaatag gtcaatcgtc cataaaacgt tacttgattg
     4861 ttgcgtggaa attccatgtt gaaatgaagt ccgtaaaaag gcttattgtt tccaaattcc
     4921 atcatagttg tagctccgcc accaacacta aattgagcag tagcttgacc taaaatcagc
     4981 ataaaactga ataaaaaggt atattttata aatctaacca tcttggtttg cttttaatta
     5041 tcttatcaaa aataatacat ttttcattat gagcaccttt taaataaccc gaactcatta
     5101 aaaattcgtt aacaatttcg ccgccaacaa acttaaaatg ctttttaaac tctttcaacc
     5161 attcttcttt ggttgtgaat tcttgcatat caagccagtt tttaaaagat ccatattgtt
     5221 tctgtaacgc aagaattgct cctgcattgt atatgacggc atctattttc aatcggttgc
     5281 gtataatgcc cgcatcattc aataaacgtt cacgttcttt atcctgataa ttagctattt
     5341 tttgaatatc aaactcttca aaagcctttc ggaacgcttg ttcttttttc aacatggtgt
     5401 cccacgacaa acccgcttga ttgatctcta aaatcaaccg accaaacaat tcattatcac
     5461 tctcaattgg aaaaccatac ttcgtatcgt ggtaaatacg atgcaaatta tccaagggta
     5521 aattttcaca ataactgcaa tacgacatcg ttcaatggat ataaaaatag gccacaattt
     5581 cttgcagcct attttggttt tttattttat ttaactaagc tttttgcttt gttttccctt
     5641 tgtctttaat ggtaattaaa ccatcttttg tttcgtttaa atccattttc aatgtatcac
     5701 cttcttttaa ttcagaattg atgatttctt ctgccaacgg atcttcaaca aatttttgga
     5761 tggcacgttt caacggacga gcaccaaatt tttcatcgta tcctttttct acaatgaaat
     5821 cttttgcggc ttcggtcaac tcaatgtggt aacctaaatt atccaaacgt tgatacaatt
     5881 tcttcaattc caaatcaatg attttgaaaa tatcttcacg ttttaatgag ttgaacatga
     5941 taatatcgtc aatacgattc aaaaactcag gagaaaacgc cttgcgcaat gcattttgga
     6001 ttaccgtttg tgcattttca tctctggatt cagcttgagc cttggttcca aaaccaacac
     6061 ccgtaccaaa atcagccaat tgacgtgctc caatatttga agtcataatc aaaatggtgt
     6121 ttttgaaatc aattttacga cccaatccat ctgtcatgtg tccatcatcc aatgcttgca
     6181 acaacaagtt gaatacatct ggatgtgctt tttcaatttc atctaacaac acaatggaat
     6241 atggttttct gcgcactttt tcggtcaatt gcccgccttc ttcataacca acatatcccg
     6301 gaggcgctcc aaccaaacgt gaaattgaga atttctccat gtattcactc atgtcaatgc
     6361 gaatcaaggc ctctgttgaa tcaaataaat attttgcaag cactttagcc agctgtgttt
     6421 ttcctacacc cgttggacct aagaagaaga atgaaccaat tggtttgtta ggatctttca
     6481 atcctgctct gttgcgttga attgctttca caaccttagc aactgcatcg tcttggccaa
     6541 tcacgcttcc ttttagttcc aaagccatgt tagccaattt cttggtttct gattctgaaa
     6601 tacgtgtaac aggaacacct gacatcattg aaacaacttc tgcaacatgg tcttctgtaa
     6661 tcgttacacg gttgtttttc gattcagctt cccatttttg tttagcagtt tccaattggt
     6721 cttgcaactt acgttctgta tcgcgcaact cagcagcttt ttcgtattgt tgcgatttaa
     6781 ttactgcatt tttctcgtct tttaaattct caatttgttg ttcaatgtcc aaaatctctt
     6841 gcggcacatt gatattggta atatgaacac gagacccaac ttcgtccaat gcatcaattg
     6901 ctttgtcggg taagaaacga tcggtaatgt aacgttctgt caacttggca catgcaacaa
     6961 ttgcttctgg cgtatatgaa accaaatggt gctcttcata acgctccttg atgttgttca
     7021 atatctgaat agtttcatca accgatgtag gttcaatgat tactttttga aaacgacgct
     7081 ccaaagctcc atctttctca atatactgac ggtattcatc taatgttgtt gcaccaatta
     7141 cttgcatttc tccacgagcc aatgctggtt tgaacatgtt agaagcatcc aaagatcccg
     7201 aagcacctcc agcaccaatg atggtgtgaa tctcgtcaat aaacaaaatc acatccggat
     7261 tgcgctctat ttcttgcatc accgccttca tgcgttcctc aaactgacca cggtatttgg
     7321 ttccagccac caacgatgtt aaatccaacg aaatcacacg tttgttaaac aatacacgcg
     7381 atacttttcg ttgcacaatg cgcaaagcca atccttcagc aattgcactt ttaccaacac
     7441 caggctcacc gatcaaaata ggattgtttt tctttctgcg cgaaagaatt tgactcacac
     7501 gctcaatttc attctcacgt ccaacaatcg ggtccaaacg tccagattca gccatacgtg
     7561 ttaaatcacg accaaaatta tccaaaacag gagtttttga tttaggatcc gttggtttgc
     7621 gttgagcgcc aaaatcttgg tcttcttctt cgccacctgc accaggaaat tcagcacgag
     7681 gcatagatga agtagaaaac ggggtgtctg gattttgtga tgacatattt aactcatctt
     7741 ttacattttc gtataaaatc ccatatttgt ttaaaacacg gcaacttacg ctttcttcat
     7801 cttttaaaat agaaagcaat aaatgctccg ttcctatcat tgaactttta aaaacttttg
     7861 attctaaata cgttctcttt aatacctttt ctgcttgttt ggttaaagga atttgtccgg
     7921 aattggtata tgcctttgaa cttgatttta atagtgtaac ttcaacttca ttgcgaatct
     7981 gacgtagatc cacatgaaat tcagtcatta tttttattgc tgtcccatct ccttcacgaa
     8041 ttaagccgag caacaaatgt tcaacgccaa taaaatcgtt tccaagacgc aaagcctcct
     8101 cgcgactata tccgataaca tctttaactc taggtgaaaa ttttgcctcc atagtttgtg
     8161 ttgttttgtg cgtataaatt tcagtattta tccgctatta caaagctaaa aaatataaat
     8221 tgttaataac ttggccaaaa agaacatttt ccacagattt ttgttagtaa ttatgttagt
     8281 aattaacctt ttttctactt ttttatatct gtaaaatgac taatttcgac acttattatt
     8341 ttgcatgcaa attgcgtgct gcaattaaaa aaatgacaag atggcagacg gagaaagaat
     8401 agtcaaaatt aacattgaaa acatgatgaa atccgcctac attgattatt caatgtcggt
     8461 gattgtgtcg cgtgcattac cagatgttcg agatggtttt aaacctgttc acagaagggt
     8521 tttatatgga atgcaagaat taggagttta ttcaaataga ccgcataaaa aatcagcaag
     8581 gattgttggg gaagtgatgg gtaagtatca cccgcatggg gatgcttcgg tttacgatac
     8641 aatggtgcgt atggcacaac actggtcgtt gcgttatcca cttgttgatg gtcaaggtaa
     8701 ctttggatct gttgatggtg atagtccagc agcaatgcgt tatacagagg caagattgga
     8761 taaaattgcc gaagatatgt tggatgattt ggaaaaagaa acggtagatt ttgctccaaa
     8821 cttcgacgaa agtttgtcgg agcctacagt tttgcctaca aaaattccta acttattggt
     8881 aaacggtgct tcaggtattg cagttggtat ggcaaccaat atggcgcctc acaatttaag
     8941 tgaagttgtt gatggaacaa ttgcctatat tgataacaac gatattacag gcgaagaatt
     9001 gttgcaatac atcaaagctc ccgatttccc aacaggtggt atcatctatg gttatgaagg
     9061 tgtaaaagat gctttgttga caggtaaagg gcgtattgta atgcgtgcaa aagctgaaat
     9121 tgaatcaaca ggaagccacg aacaaattat tgtaacagaa attccatacc aagtaaataa
     9181 agctgaaatg attaaacgta cagctgattt ggttaacgat aaaaaaattg aaggaatttc
     9241 agatattcgc gatgagtcgg atagaaacgg gatgcgtatt gtgtatgaat taaaacgcga
     9301 tgcaattcca aacgtagtgt tgaacaaatt gtttaaatac actgcgcttc aaacatcttt
     9361 ttcggtaaat aatatttgct tggtggatgg tcgcccacaa ttattaaata ccaaagaaat
     9421 gttgatgcat ttcgttgatt tccgtcacga tgttatttat cgtcgtacgc aatatgattt
     9481 gagaaaagcg gaggaacgcg ctcatatttt agaaggttta atcattgctt cggataacat
     9541 tgatgaagta attaaaatta ttcgtggtgc cgaaagtcca caagcagcta ttgaccaatt
     9601 gatggcacgt tttagcttgt ctgaaattca agcaaaagct attgttgaca tgcgtttgcg
     9661 tcagttgact ggtcttgagc aagataaatt gcgtgctgaa tatgctgact taatgaaatt
     9721 gattgaagat ttgaaagata tcttggcaaa caaaagccgt atcatgcaaa tcatcaaaga
     9781 tgaattgact tatgtaaaag agaaatacgg cgacgacaga cgctctgaaa ttgaatatgc
     9841 tgcaagcgaa atgcgtattg aagatttaat tccagatgaa gaagtagtgg ttaccatttc
     9901 tcacgcagga tatattaaac gtacaagtct ttcggaatat aaagttcaga acagaggtgg
     9961 tatggggtcg aaaggatctg caacacgcga taaagacttc ttagaagaat tatttgttgc
    10021 aaccaaccac aattacttat tggtatttac cgaaaaagga aaatgtttct ggatgcgtgt
    10081 attcgaaatt ccagaaggca acaaaacttc caaaggtaga gccgtacaaa acttaattaa
    10141 catcgaacaa gatgataaaa ttaaggcgta tgtaaaagtg caagatttaa ccgatagtga
    10201 atacgtggat aataacttca ttatcatgtg tactaaaaat ggtgtgatta agaaaacatc
    10261 gttggaagca tattcacgtc ctagagctaa cggtatcaat gccattacag taagagatgg
    10321 agatgaattg ttggaagcta aattgaccaa tggaactaac gagattgttt tggcaactcg
    10381 cgagggtaga gcaattcgct ttaacgaaca aacagtgcgt ccgatgggta gaaatgcttc
    10441 aggtgtgcgt ggtgtaacgt tgactagcaa aactgatgct gttattggta tgatttgcgt
    10501 tgaagatgtc tattcaacag taatggttgt ttctgaaaaa ggatacggaa aacgtacgtt
    10561 cttaaatgat ccagaagacc aagaaccaat ttaccgcatt accaaccgtg gtggtaaagg
    10621 ggtgaaaaca ttaaatatta ccgataaaac tggtgcatta ttgtctatca aaaatgtaac
    10681 ggatgaagaa gatttaatga ttatcacaaa agcgggaact actatccgta tgcacattga
    10741 ttctatccgt acgatgggta gagcggcaca aggagttcga ttaatcaact taaaaggaaa
    10801 tgctgaaatt gctgctgttg ctcgtgtgcc aagaagtgat gaagaagatg aagaaggaga
    10861 atttgatgaa acaaacacgc ctttgaatga tgctcctgaa gaaaatggca caacaattga
    10921 agatggaaat gaagaataat taattgcata aaaatgaaaa caaataaaat tacagtttta
    10981 gcaggatgtt tgatggtttc ggcattgagt tttggtcaaa aaaccaatgt tacaagtgct
    11041 gctgttgaat acaaacaaaa atttcaacca tcgttgatga tgggaaaagt ggatgatgca
    11101 aaacaagcct tgttgaacgc taaaaaatac attgatttag ctgccgaaca cccagataca
    11161 aaagcagacc cgaaaacgtt gtattataaa ggtgaaattt atggtggtgc tgttcagtta
    11221 gcatctatga ctgctgatac caactttatg attaacaatt ttggtaaaga tgcaatggag
    11281 gtttctatta aatcattgca agaaagttat aaaatgagta gcaaattccg cccagatatc
    11341 gaccaatcgg ttaatatgca agttggaatg ttatcgccaa tggctagtaa attctacgaa
    11401 gaaggaaaat acaaagaggc tggtgctgct ttttatttca tctataaagt aacaacagct
    11461 aaaaatgagt ttgatacaac taacttgtac aatgcagctg tatgtttaga aaaagcagat
    11521 atgattaaag aagcagctga aacttattct gaattagctg aaattggcta caaaaaagga
    11581 gaaggttatg catttgctgc taacatgtat accaaattaa aagattacga taaagcaaaa
    11641 gaaattcttt taaaaggaaa agctaaatat ggtaacgata aagcaatctt gttagaattg
    11701 gttcgtgtgg atatggcaaa aggcgataat gcagctgcag aaaaatcatt atcagatgca
    11761 attgctgccg atcctaaaaa caaacaattg tattacatca tcggaacaat ttatacagaa
    11821 ttgggtgaaa acgaaaaagc tgaaaaagca ttgttgagtg cgttggaaat tgaccctaac
    11881 tatttggatg cacaatacaa cttgggcgca cactacgtaa cttgggcaac tcaattgaga
    11941 gataaagcaa atacattgaa cgaaaacgat ttcaattatg atatgttgtt gtctcaaagt
    12001 agagatttgt acaaaaaagc aattgctcca cttgaaaaat acattgtgtc tcagccaaaa
    12061 gacgcaaacg tattattgat tttattccaa attaaccaaa acttaggaaa ttcggataaa
    12121 gcaaaagaat acaaagcgcg ttacgatgcg gcaaaataat ttgttgaaat tttaacaatt
    12181 agaaagggaa gttttttttt gaacttccct tttttattga atagaactct tacttttgaa
    12241 ctcgttaaac ttatttttta cacaatgata acagaagaaa aaatcaaagc atcggaaacg
    12301 cgaattttta aagcggtttt ccccaacact accaatcatt acgatacact ttttggaggt
    12361 acagctatgc aactaatgga tgaggtagca tttatcactg caacacgttt tagtagacaa
    12421 cgcatggtaa cggtaagcag tgatagaatc gattttaaaa aacctattcc agctggaacc
    12481 atcattgaat tggtaggaaa agtgacctat cttggaagca caagccttaa aattcgcgtg
    12541 gaaatttata tcgaacaaat gtatgaagaa caccgtgaaa aagccattag tggagagttt
    12601 acatttgttg ccattgatga aagtaaaaaa cctgtaaaaa ttcaataagt agaagctgca
    12661 tttgtaattg tttattagca atacaatttg tatcttcgca atcaaatttt tttaatgatg
    12721 aagcaaatta ctttaaacga tattcacgta gctcttggag ctaagatggt tgattttgca
    12781 gggttttaca tgcctgttca atacgaaggt gttaatgttg aacacgaaac tgtacgcaat
    12841 ggcgtagggg tgtttgatgt gtcacacatg ggagagtttt taatttcagg aaaaaatgca
    12901 ttggcattga ttcaaaaagt agtttctaac gatgcatcaa cattaacaga tggtagagct
    12961 caatatgcat atttacctaa cgaaaatgga gggattgtag atgatttgat tatttaccgt
    13021 ttcaacgaag aaaaatattt gttggttgta aatgcttcaa acattgataa ggattggaat
    13081 tggatttcta aattcaacca agaaattggt gcagaaatga gaaacctttc ggatgattat
    13141 tctttgttag ccatccaagg accaaaagct gcagaagcta tgcaatcatt aacttcagta
    13201 aatttgtcag ctattccata ttaccatttt gaagttggat attttgctgg ttttgaagat
    13261 attatcatct ctgcaacagg atatactgga agtggaggtt ttgaaatcta ttgtaaaaac
    13321 gatcaagtta aacaaatttg ggataaagtt tttgaagcgg gagcatcgtt tggcattaaa
    13381 ccaattggat tggcagcacg cgatacgttg cgtttggaaa tgggattctg cttgtatgga
    13441 aacgatattg acgataccac ttcgccttat gaagctggat tgggatgggt aacaaaattt
    13501 acaaaagatt ttgtgaatgc cgatttctta aaagctcaaa aagaaaatgg ggttagccgt
    13561 aaattggttg cttttgaatt gattgataga ggaattccac gccatgatta tgaaataact
    13621 g
//
LOCUS       DAOQVQ010000010        13544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5139_length_13544_cov_4.286678, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000010 DAOQVQ010000000
VERSION     DAOQVQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_13544_cov_4.286678"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKN22_00720"
     CDS             <1..909
                     /locus_tag="PKN22_00720"
                     /inference="COORDINATES: protein motif:HMM:NF027450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HNS41253.1"
                     /translation="INYWQKEDITTLEEQVEQKFPPIDEIKRVYRAVCNFLKIAIGSG
                     KDETYAFNISQFSKNFDISPALCFNSLKILELTGELSFSEGFFSPTKLKFSIGNEALY
                     NFQIQHETFRPLITIITRAYPGVFMNFMEINESEICKRLNIQPKELETKLQFLEKTGI
                     IDINWKTDSPTITFTRERMPYDYLEIDPEVYHFRKKIAVEKLVSVKKYLQDDVCRSMQ
                     LIHYFGLPTEKCGKCDVCINEKEQENNPNYISMVLNFLQENPKKDFEVKALFTNDALY
                     KTTIRKLMVEEKIKFDGENYTLFTAN"
     gene            complement(906..1451)
                     /gene="rsmD"
                     /locus_tag="PKN22_00725"
     CDS             complement(906..1451)
                     /gene="rsmD"
                     /locus_tag="PKN22_00725"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNS41254.1"
                     /translation="MRIIRGTLKTKKITVPKNFPSRPTTDFAKEGLFNMLENRLDIDG
                     CDLLDLCAGTGNISFEFLSRGAKNVVAVDSNFNVTRHIRHLAKEFELLPSIQIVHNDI
                     LKYLEKTEQKFDVVFADPPYEFKHYEQLVQLVLEKQLLKEDGLLIVEHGKRTAISHLK
                     GYESSRGYGNVVFSFFTFSHD"
     gene            complement(1442..2194)
                     /locus_tag="PKN22_00730"
     CDS             complement(1442..2194)
                     /locus_tag="PKN22_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNS41255.1"
                     /translation="MTHLAIDINTNAVRFVKLNGNFVVSERAFAFQEKQDYRYKAQLE
                     EFWKQTEWKETDFEKATLSWSEKQNVVVPANVFNESDKNAIFNLAYGTSVSLDEVDYN
                     RLPMQNMVNVFFIPAWVKSFFVLRFPRIVLQHEATHLIRGVFEGPTFKLKSTLVLHQE
                     HLVLMVLKENNLKFYSSFDYATLEDVVYYFSYSMQQLELNEQPGELEITNGVGSEVDL
                     AALQALIEKVQGNNCTVKQSHSLVEKYQSTCV"
     gene            2352..3506
                     /locus_tag="PKN22_00735"
     CDS             2352..3506
                     /locus_tag="PKN22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNS41256.1"
                     /translation="MEDISLFFAPVAQDRVIDFGLGKTIDTHTESAGFPEIPKQSIAL
                     FYVPEFRNSNFEKVKHEKFREQLHQFNYGKDWTRKIVDLGDVFPGESINDTYFAVSKI
                     VATLVKHDVIPVIIGGSQDLTMAVYKGYETLEQYVNLCTIDAKLDMGTPENELHADGY
                     ISHLLVQRPCYLFNHSNIGLQIPYADKDELDLFDKLYFDYCRLGEYNADFKRAEPHLR
                     NSDILSIDFSSIRYGELADSQYSNANGFYADQMCQIARYAGVSDKLSSMGIFNYLPEL
                     TNATNASNLVAQIIWYFIDGVNARVGDYPIGNRKNYLKFIVHIDDFKDEIVFFKSDKS
                     ARWWMQVPYPAEEQRKYERHYLVPCNHEDYDRAMQNEIPDLWWKTYQKLV"
     gene            complement(3515..4057)
                     /locus_tag="PKN22_00740"
     CDS             complement(3515..4057)
                     /locus_tag="PKN22_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNS41257.1"
                     /translation="MKIKAKFIVYFVVLLLASSWTYGQAPATQADTLVSTTKDSTSTK
                     KRDEHRIDKMLAFAKSLIGTPYRYSGMTPSGFDCSGFVNYVVGHFGFSIPRPSYLIAE
                     QGETIKLSDVKPGDLLFFKGSDVRSNRVGHVAMVVSRNETEINIIHSSNGKGVAIENI
                     IKSKYYIPRFIKAKRMSYSY"
     gene            4105..4467
                     /locus_tag="PKN22_00745"
     CDS             4105..4467
                     /locus_tag="PKN22_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNS41258.1"
                     /translation="MAANFDIKDLKKLYYTIGEVADLFHVNQSLIRFWEKEFPSIQPK
                     KNAKGNRLFTPKDIETFNKIYTLVKVQGFTLDGAKNELKKRKSTLIDNETPIEKTPNN
                     EVIAKLEAIKAKLIALKK"
     gene            4533..7184
                     /locus_tag="PKN22_00750"
     CDS             4533..7184
                     /locus_tag="PKN22_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS41259.1"
                     /translation="MHKLTLLFLVLLSNFILAQEVEITGQCINSKKEGISDILIRINQ
                     RNEAIYTDQNGNYSTKANVGDTLNIHYFVSGDTTQRQQVFFVKNNHSTKIPAFEINIK
                     QFEDVKIIKEIDKPFDLTPIKVKDWQTLPLQSVEHVLVFTTAARSNNELTSNYNVRGG
                     NYDENLVYVNGFQIYRPFLTRAGQQEGMSFINSALVESLDFSAGGFDAQYGDKLSSVL
                     DIKYRKAEKFKGSILASLLGVEGHLEQQLGKKKRFSYMLGARFRSNGYFLNALPTKGA
                     YNPIFWDAQLLTDYIISENWTWSILGHISSNNYTFSPQTQKTDFGTANEAYSFMVYFD
                     GKEQTRFRTMMGGTKFAYTSNDNKTKLNFFASAFHTDEREYFDVQGQYYINELETDPS
                     KEEFGDSIAVLGVGTFLNHARNRLRATIFNVYHTGSKELKNTFVDSERTLHRQYNILW
                     GVNFQQNDFYDVLSEWKMIDSAGYSLPQTNDHKVNLQEVIKGKLNLQSSVINGYTQFN
                     GIWSKTKVNYPVSITKKIKIKKQEPIKVTFRDTIDASSSKWALSIGTRVSYTSVNNEF
                     FVTPRASVMYYPRAYLVKDSTITRRNMSLRFSTGLYYQPPIYRELRTFDGHLNKNVLS
                     QKSYHAVVGYDMFFFMWGRHSPFKFTTELFYKYMWDVNPYEVENVRTRYYANNNATAY
                     AYGIDFNINGEFIEGISSYFKIGLMRTMENIKDDQYTEYYNAAGEKIIFGYSDDQQVV
                     DSAVIQPGYIPRPTDQWLNIGLLIQDKMPKLEALSLQLGLQFGTPLPYGPPGHDRYKD
                     TLRMKPYFRVDIGTSYDFLHNKHKQKPNFFNKAFTDAILSFEVYNLLGFKNEMSKQWI
                     QDVNGGYYSIPNYLTSRRFNLKLILRF"
     gene            7237..7557
                     /locus_tag="PKN22_00755"
     CDS             7237..7557
                     /locus_tag="PKN22_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNS41260.1"
                     /translation="MADINVIIIDREGVEHNLVAPTDMNMNIMELCKSYDLPVKGECG
                     GMAMCATCQCYLESDHQLDEQSDAELDMLDQAFFVKSNSRLGCQIPITEEIDGIRLRL
                     APEA"
     gene            complement(7554..8330)
                     /gene="nadE"
                     /locus_tag="PKN22_00760"
     CDS             complement(7554..8330)
                     /gene="nadE"
                     /locus_tag="PKN22_00760"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNS41261.1"
                     /translation="MKAEAVSQHIQQWLKNYLDSTGVKGFIVGISGGIDSALVSKLCA
                     LTGEKTLVISMPIRQTEAEYSRATKHIETLKAQHSNVIGLEVDLTPTFTQFEKDIPFD
                     CEQNHLAMANSRSRLRMMTLYAVGQANGCLVVGTGNKIEDFGIGFFTKYGDGGVDISP
                     IADLTKSQVFALARHLEVIPEILAAKPTDGLFADGRSDEDQIGATYPELEWAMNFSGD
                     ETKLSEREATVLAIYRRLNRINQHKMQPIPVCTIPTDLLD"
     gene            complement(8341..9162)
                     /locus_tag="PKN22_00765"
     CDS             complement(8341..9162)
                     /locus_tag="PKN22_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS41262.1"
                     /translation="MKKIVLGLFALTTALTTLAQSAAEKKFQGGITAGFGINFQNMGT
                     KFIEKDGVGTDLFIGGAMHYGFTDNVGLATGLEFTFSSAKFKTGPNTLLYYYNDTKIV
                     GVDDFDADHTSANDAFVLSKRKQNATYINIPTMLLFRTNFIGYVRYFGKLGMKHNFLL
                     TQKSQDTGYSLSTQGMDLTKLGEEVTQKNMKAKNEMVFYKGSVGIAGGAEWNFTGNTC
                     LVAELGFYYGVTPLYYNRKNSYLFTYETENNSIVRSKVNNKATQAQLELKVSILF"
     gene            9310..10248
                     /locus_tag="PKN22_00770"
     CDS             9310..10248
                     /locus_tag="PKN22_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HNS41263.1"
                     /translation="MDVDTSNVKLEIQYVNLDSLLFNTPTDKLIDLRQQMGQKIPELF
                     DYQIGYCMRIGKVVDTAFVNSIVQYRQDTMIQKLEKEIAHKFSNLSAQKSNLTTAFKR
                     LKAHIKTANVPTSIVFQNSLFTSSAFCTEQEIGIGLERYLGAQSPIIKSLPGEPFYDW
                     IKQGYDVQFLERDAVLSWILTHIIPEIKGNLAELMIYHGKALYLTEACLPESEKNIII
                     RYSKEDLKWSQENEYSLWKYLVDEKILFKTDDLIAKNMLQEGPFTSGLPEKGPDRLGQ
                     YLGWVIVKNYMENSETTFEELINTPYNEILQSYQPK"
     gene            10268..10621
                     /gene="gldC"
                     /locus_tag="PKN22_00775"
     CDS             10268..10621
                     /gene="gldC"
                     /locus_tag="PKN22_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNS41264.1"
                     /translation="MSQEDKIVKTSEIVLKVGMNENNLPLKIKWTAKDGDVEDKPARA
                     MLLSVWDHNENNTMKIDLWTKDMSVEEMKQFFHQTLLTMADTFERATGERNISEDLRD
                     YCYHFADKMQILPEN"
     gene            10621..11409
                     /locus_tag="PKN22_00780"
     CDS             10621..11409
                     /locus_tag="PKN22_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HNS41265.1"
                     /translation="MKFITRKMNAVLTDTVEYSFRIGSDKIVVNDYIGKPIRLAFSGA
                     IYCVNCSKKINKSFQGFCFNCFQTAANAAECIIHPELCRAHLGEGRDVEWEQANHNQP
                     HVVYLALSSEIKVGVTRTTQVPTRWIDQGASAAIRLMETPNRFEAGRIEVALKSIFTD
                     KTNWQKMLKNEINETIDLVEEKWRLQEQLPDDLTTYFSEDDELIEINYPVLQYPKKIN
                     SLSFDKQAVIEKTLIGIKGQYLLFDDQSVLNIRKHEGYEIEFLG"
     gene            complement(11406..12533)
                     /locus_tag="PKN22_00785"
     CDS             complement(11406..12533)
                     /locus_tag="PKN22_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS41266.1"
                     /translation="MLNINQLSFWERKTYFEGIDFLIIGGGIVGCSTAYHLKMKFPTA
                     KIVVLERGYLPSGASTKNAGFASFGGPVELVEDLKKHPASLVWETIQARYEGLIYLRE
                     LIGDAAMDFQANGGWDLIDHQQTAIATEVRDNLNELNTEFIRLTGKKGFEEDAQAIQR
                     FGFHQLSTAFKILQEGQIDTGKMMHRFYQLLAEKGVLLLNGIEVETLGANYNSVETTI
                     GQITASNVVITTNGFTQKLVKELDVNPARAQVLVTSPISNLQLKGTFHYDAGYYYFRN
                     FENRILLGGARNLDFEGETTYEMTTTPLIQNRLVELLKTMIIPNTQFTIDYQWAGTMG
                     MGTSKFPIIQKINDNCAVGARLGGIGVALGTNVGKKVAALF"
     gene            12704..>13544
                     /locus_tag="PKN22_00790"
     CDS             12704..>13544
                     /locus_tag="PKN22_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41267.1"
                     /translation="MNVKFDGAVSNFDKGSSESGVTVSIIQNGQAVSSATTSSNGRYS
                     VKGAIDIKKPFDVVFKKSGFVSKKVSFNFIGLNLEDTPAGDFKPVDKLDLDIFAERPG
                     LDFSFLNTQSVGEFTWNSQGYVEVNDAKRKAVADKIDNLLKKSEQDAQNNQAAYNAAI
                     QAADKAFNAKDYTAALAKYEEATKYKPKETYPIQRIDEIDALLQKQKEDALKFQQENA
                     AYLNLIKAADNFFKLGDYEKAKQKYYEASDISDEQYPLDQIKEINRLVKEKENEAKYK
                     QLIE"
BASE COUNT         4513 a         2536 c         2362 g         4133 t
ORIGIN      
        1 atcaattatt ggcaaaaaga agatataacg acgttagaag aacaagttga acaaaaattc
       61 cctcctattg atgaaatcaa acgggtgtat cgtgctgttt gcaattttct aaaaatagcc
      121 atcggttctg gtaaagatga aacctatgcg ttcaacatat ctcagttttc taaaaacttt
      181 gacatctcac ctgcactttg cttcaattca ttgaaaatct tagaattgac gggcgaactt
      241 tctttttccg aaggtttttt ttcgcctaca aaactaaaat tctcaatagg caatgaggcc
      301 ttgtataatt tccaaattca acacgaaacg tttcgtccgc ttattaccat cattacacgc
      361 gcctatccag gtgttttcat gaattttatg gaaattaatg aatcggaaat ttgcaaacga
      421 ttgaacatcc aaccaaaaga attggaaaca aaattgcaat ttttagaaaa aacaggaatc
      481 atcgatatca attggaaaac cgattcgcca accatcacct ttacacgcga acgtatgcct
      541 tatgattatt tggaaattga ccctgaagtg tatcattttc ggaaaaaaat tgccgtagaa
      601 aaattagtga gcgtcaaaaa atacttgcaa gatgacgtat gtcgttcgat gcaattgatt
      661 cactattttg gacttcctac cgaaaaatgt ggtaaatgcg atgtgtgtat aaacgaaaaa
      721 gaacaagaaa acaatcccaa ttacatttca atggtgttga attttttaca agaaaatcct
      781 aaaaaagatt ttgaagtaaa agcattgttt accaatgatg cactttacaa aacaaccatt
      841 cgaaaactaa tggtagagga aaaaataaaa tttgatggcg aaaattacac gttgttcact
      901 gccaattaat cgtgtgaaaa tgtaaagaaa ctaaaaacca cattgccata gcctctgctc
      961 gattcatagc cttttaaatg cgaaatagct gtgcgcttgc cgtgttctac tatcaacaat
     1021 ccgtcttctt ttaacaattg tttttccaaa accaattgca ccaattgctc ataatgctta
     1081 aattcatacg gcggatcggc aaaaacaaca tcaaattttt gttctgtttt ttctaaatat
     1141 ttaagaatat cgttgtgcac aatttgaata gacggcaaca actcaaactc ttttgctaaa
     1201 tgacgaatgt gacgggttac gttaaaatta gaatcaaccg ccactacatt ttttgcacca
     1261 cgcgataaaa actcaaacga tatatttcca gttcctgcgc acaaatccaa caaatcacac
     1321 ccatcaatat ccaaacggtt ttccaacata ttgaacaatc cttcttttgc aaaatcggta
     1381 gttggtcgcg aaggaaaatt tttcggaacc gtgattttct ttgtttttaa tgttcctctg
     1441 attatacgca cgttgattgg tatttttcta cgagtgaatg actttgtttg accgtgcaat
     1501 tgtttccttg cactttttct attaatgctt gaagagcggc taaatcaact tccgaaccta
     1561 cgccattggt tatttccagt tcacctggtt gttcgttcaa ttccaattgt tgcatgctgt
     1621 acgaaaaata atacaccaca tcttccaatg tagcataatc aaacgaagaa taaaatttta
     1681 aattattttc tttcaacacc atcaatacca aatgttcttg atgcaaaacc aaggttgatt
     1741 tcaacttaaa tgttggtcct tcaaaaaccc cgcgaatgag atgtgtggct tcgtgttgta
     1801 atacaatgcg tgggaagcgt aacacaaaaa acgatttcac ccaagcagga ataaaaaata
     1861 cattcaccat attttgcatc ggcaaacggt tgtaatctac ctcatccaaa gacaccgaag
     1921 taccataagc taaattgaaa attgcatttt tatccgattc gttaaacaca tttgctggaa
     1981 ccaccacatt ttgtttttca gaccacgaaa gagttgcttt ttcaaaatca gtttctttcc
     2041 actcggtttg tttccaaaat tcttccaatt gtgctttgta acgataatct tgtttttctt
     2101 gaaacgcaaa agcacgttca ctcaccacaa aattaccatt cagcttaaca aagcgcacgg
     2161 cattggtatt gatgtctatt gctaaatgtg tcattccact tttacttggt agcacaaatc
     2221 tacacttttt gttcaatata tgttgcattt cacaaaaacc taaacatctc aacaatttat
     2281 cgttcatagt tttattaggc aacgcctcca acagaaaaat tttaagttta gttttgaaac
     2341 aaataattat tatggaagat atttcgttgt tttttgctcc tgttgctcaa gatcgtgtaa
     2401 ttgattttgg acttggaaaa accattgata cacatacaga aagtgctgga tttcctgaaa
     2461 tacccaaaca aagcattgcg ctcttttatg ttcctgagtt tagaaattca aactttgaaa
     2521 aggtaaaaca cgaaaaattc cgtgagcaat tacaccaatt caactatgga aaagattgga
     2581 ccagaaaaat tgttgattta ggcgatgttt ttcctggcga atccatcaac gatacttatt
     2641 ttgcggtgag taaaatcgtt gcaactttgg ttaaacacga tgttatccct gtcattattg
     2701 gtggttcaca agatttaaca atggcggttt acaaaggcta tgaaaccttg gaacagtatg
     2761 taaacttgtg tactattgat gcaaaactcg acatggggac acccgaaaac gaattgcatg
     2821 ccgacggata catcagccat ttgttggtgc aacgtccatg ttatttgttc aaccattcca
     2881 acattggctt acaaattccg tatgccgata aagatgaatt ggatttgttt gataaattgt
     2941 attttgatta ctgccgtttg ggcgaataca atgccgactt taaacgcgcc gaaccacatt
     3001 tacgcaattc agatatttta agtattgatt tttcaagtat tcgttatgga gaattagccg
     3061 attcgcaata cagcaatgcc aatggttttt atgccgatca aatgtgtcaa attgcccgct
     3121 atgctggagt aagtgataaa ttatcatcaa tggggatttt taattattta cccgaactaa
     3181 ctaatgcaac caatgcttct aatttggtag ctcaaattat ttggtatttt attgatgggg
     3241 taaatgcgcg tgttggcgac tatcctattg gcaaccgtaa aaactaccta aaattcatcg
     3301 tccacattga tgattttaaa gatgaaattg tatttttcaa aagtgataaa tctgctcgtt
     3361 ggtggatgca agtaccttat cctgccgaag aacaacgtaa atacgaacgt cattacttag
     3421 tgccgtgcaa ccatgaagat tacgacagag caatgcaaaa tgaaatacct gatttgtggt
     3481 ggaaaaccta tcaaaaatta gtgtaacacc ttgattaata gctatacgac atacgttttg
     3541 cctttataaa acgagggatg tagtatttac ttttgatgat attttcaatg gcaacacctt
     3601 ttccgttgga tgaatgaatg atgttgattt ctgtttcatt tcttgaaaca accattgcca
     3661 cgtgtccaac tctgttacta cgcacatcac ttcccttgaa aaacaataaa tcacctggtt
     3721 ttacatccga taatttaatg gtttcacctt gttcggcaat taagtaactt ggacgtggaa
     3781 ttgaaaaccc aaaatgtcct acaacataat ttacaaaacc agaacaatca aaaccagatg
     3841 gcgtcatacc tgaataacga taaggcgtac caatcaatga ttttgcaaag gccaacattt
     3901 tatcaatgcg gtgttcatcg cgttttttgg tggaagtgga atcttttgta gttgaaacaa
     3961 gtgtatccgc ttgcgttgct ggtgcctgac cataagtcca gctacttgcc aacaatagca
     4021 caacaaaata aactataaat ttcgccttaa ttttcatttg gttgcaaaaa tataattaat
     4081 ttcgaaaaaa taaaaattga gtagttggca gcaaattttg atattaagga cttaaaaaaa
     4141 ctctattaca ccattggtga agtagccgat ttattccacg taaaccaatc ccttattcgg
     4201 ttttgggaaa aagagtttcc cagcattcaa ccaaaaaaaa atgctaaagg caaccgactt
     4261 ttcacaccaa aagacatcga aacttttaac aaaatttata cattggtaaa agtgcaaggc
     4321 tttacactcg atggagcaaa aaatgaatta aaaaaacgaa aatctactct aattgacaac
     4381 gaaacaccca tcgaaaaaac gcctaataac gaagtaattg ccaaattaga agctataaaa
     4441 gcaaaattaa ttgccctaaa aaagtagtgg attgtacaat tattcaaaat aaacctgctc
     4501 aaattccgtt tctagagtta tttttgtcaa ctatgcataa attaaccttg ctttttttag
     4561 tgttactctc taatttcatt cttgcacaag aagtggaaat tacggggcaa tgtattaatt
     4621 ctaaaaaaga aggtatttct gatatcttaa ttcgcatcaa ccaacgaaac gaagctattt
     4681 acaccgatca aaatggaaat tattctacaa aagcaaacgt tggagataca ttaaacattc
     4741 attattttgt ttcgggcgat acaacccaac gccaacaagt attttttgta aaaaacaacc
     4801 attccaccaa aattccagcg tttgaaatca acatcaagca atttgaggat gtgaaaatca
     4861 tcaaagaaat tgacaaacca tttgatttaa cccccatcaa agtaaaagat tggcaaacat
     4921 tgccgctaca aagtgttgaa cacgtattgg tttttacaac agctgctcgt tccaacaatg
     4981 agttaacgtc caactacaac gtgcgcggtg gtaattacga cgaaaacctg gtttatgtca
     5041 acggatttca aatttacaga ccgtttttaa cgcgagctgg acaacaagaa ggaatgagtt
     5101 tcatcaacag tgctttggtg gaatcgttgg acttctctgc aggtggtttt gacgcacaat
     5161 atggtgataa attgagttct gtattggaca ttaagtaccg taaagccgaa aaattcaaag
     5221 gttcaatttt agcttcgtta ttgggtgttg aggggcattt ggagcaacaa ttgggcaaaa
     5281 agaaacgctt tagctatatg ttgggagcgc gtttccgttc caacggttat tttttaaatg
     5341 cacttccaac aaaaggagca tacaacccta tattttggga tgcgcagctt ttaaccgatt
     5401 acatcatttc agaaaattgg acatggtcga ttttaggaca catatcgagc aacaattaca
     5461 cattttcgcc acaaacacaa aaaacagatt ttggtactgc caacgaggcc tactcgttca
     5521 tggtttactt tgacggtaaa gaacaaacac gttttcgcac tatgatggga ggaacaaaat
     5581 ttgcctacac atccaacgat aataaaacca agttaaactt ttttgcttcg gcctttcata
     5641 ccgatgaacg tgaatatttt gatgtgcaag ggcaatacta catcaacgaa ttggaaaccg
     5701 acccatccaa agaagaattt ggagactcca ttgctgtgct gggtgttgga acgtttttaa
     5761 atcacgcacg taaccgcttg agagcaacaa ttttcaacgt ttaccatact gggagcaaag
     5821 aattgaaaaa tacgtttgtt gattccgagc gcacattgca ccgtcaatac aacattttgt
     5881 ggggagtcaa ttttcaacaa aatgattttt acgatgtgtt gagcgaatgg aaaatgattg
     5941 actctgcggg ctatagttta ccacaaacca acgatcataa agtcaatttg caagaagtta
     6001 tcaaaggaaa attgaacctg caaagtagtg ttatcaacgg atatacgcag ttcaacggca
     6061 tttggtctaa aacaaaagtg aactatcctg ttagcatcac caaaaaaata aagataaaaa
     6121 aacaagagcc aataaaagtt acatttagag atacaattga tgcgtcttcg tcaaaatggg
     6181 ctttatccat tggaacaagg gtttcataca ccagtgtaaa caatgagttt tttgttactc
     6241 cacgtgcttc ggtgatgtat tatcctcgtg cttatttggt gaaagattct accattacac
     6301 gaagaaacat gagtttgcgt ttttcaactg gtttgtacta ccaaccacca atttatcgtg
     6361 agttgagaac ttttgatggt cacctcaata aaaacgtctt atcacaaaaa tcgtatcatg
     6421 cagtagttgg ttacgacatg tttttcttca tgtgggggcg tcacagtccg tttaaattca
     6481 caacagaatt gttttacaaa tacatgtggg atgtcaaccc atacgaagtt gaaaatgtgc
     6541 gcacacgtta ttacgccaac aacaatgcaa cggcttatgc ctatggtatt gatttcaaca
     6601 tcaatggtga atttattgaa ggcatttcct cttattttaa aattggattg atgcgtacta
     6661 tggaaaacat caaggacgat cagtacaccg aatattacaa tgctgcgggc gaaaaaatca
     6721 tttttggtta cagcgatgac caacaagtgg tggattctgc tgttattcaa ccaggttaca
     6781 ttcctcgtcc caccgatcaa tggctcaata ttggtttgtt gattcaagac aaaatgccca
     6841 aattagaagc attgagcttg caattaggtt tgcaatttgg aactccgctg ccttatggac
     6901 ctccagggca cgatcgttac aaagatactt tgcgcatgaa gccttatttt agagtagata
     6961 ttggtacatc gtatgatttc ttgcacaata aacacaaaca aaaaccaaac ttcttcaata
     7021 aagccttcac cgacgcaatt ttatcgtttg aagtttacaa cttacttggc tttaaaaatg
     7081 aaatgtctaa acaatggatt caagatgtaa acggagggta ttattccatt ccaaactact
     7141 tgaccagtcg ccgattcaat ttaaaattga tacttcggtt ttaaatcatt tgaaaaaaaa
     7201 gaagttatcc gtatctttgc gccaatttta gaaatcatgg cagatattaa cgtaattatt
     7261 attgacagag aaggtgtaga gcacaattta gtagctccta ccgacatgaa catgaacatc
     7321 atggaattgt gtaaatctta cgatttacct gtgaaaggag aatgtggtgg aatggcaatg
     7381 tgtgccactt gtcaatgtta tttagaatct gaccatcagt tagacgagca atcggatgct
     7441 gaattggata tgctcgacca agcatttttt gtgaaatcta acagtagatt gggttgtcaa
     7501 attccaatta cagaagaaat agacggaatt cgtttgcgtt tggctcctga agcctaatcc
     7561 aacaaatcgg ttggaattgt acacaccggt attggttgca ttttgtgttg attgatgcgg
     7621 tttaatcttc ggtaaatagc taaaaccgtt gcttcacgtt ctgacaattt agtttcatcc
     7681 cccgaaaaat tcattgccca ttccagttcg ggataggttg caccaatttg atcttcgtcg
     7741 cttcgtccat cggcaaataa gccatcagtt ggttttgctg caagaatttc tggaataact
     7801 tcaagatgac gtgccaaggc aaaaacttga gattttgtta aatcggcaat aggggaaata
     7861 tcaactcccc catcaccata ttttgtaaaa aatccaattc cgaaatcttc aattttgttt
     7921 cctgtgccaa ccaccaaaca accattggct tgccccactg catacaaagt catcatgcgc
     7981 aaacgcgaac gtgaatttgc cattgccaag tgattttgtt cacaatcaaa aggtatatct
     8041 ttttcaaatt gggtaaaagt tggcgttaaa tcaacctcta aaccaatcac attggaatgt
     8101 tgtgctttta gtgtttcaat gtgttttgta gcccgtgaat attcagcctc cgtttgacga
     8161 ataggcatgg aaatcaccaa ggttttctcg cccgtgagcg cacacaattt tgaaaccaat
     8221 gccgaatcaa ttccgccaga aattccaaca ataaatcctt tcactcctgt agagtccaag
     8281 tagtttttta accactgttg aatgtgctgt gaaactgctt cagctttcaa aatagaataa
     8341 ttagaataaa atagatactt tcaattccaa ttgcgcttga gtagctttat tatttacttt
     8401 acttctaact atcgaattat tttctgtttc gtaagtaaat aaataagaat ttttgcgatt
     8461 gtaatacaat ggtgttacac cataataaaa accaagttct gccactaaac aagtatttcc
     8521 agtaaaattc cactctgctc caccagcaat accaacagaa cctttgtaga ataccatttc
     8581 atttttagct ttcatgtttt tttgagtaac ttcttcaccc aatttggtca aatccattcc
     8641 ttgagtagaa agtgaataac ctgtatcttg tgatttttga gtcaacaaga agttgtgttt
     8701 cattcctaat ttaccaaaat aacgaacata tccaataaag ttggttctaa acaacaacat
     8761 ggttggaatg ttgatataag ttgcattttg ttttcttttg ctcaaaacaa aagcatcgtt
     8821 tgcacttgtg tgatctgcat caaaatcatc tactcccact atttttgtgt cattgtaata
     8881 atacaataat gtgttaggcc ctgtcttaaa tttagcactc gaaaaggtaa attctaaccc
     8941 agttgctaat ccaacattat ccgtaaaacc ataatgcatt gctccaccga taaataaatc
     9001 ggttcctaca ccatcttttt ctataaattt ggtgcccatg ttttgaaaat tgataccaaa
     9061 tccagcggtg ataccacctt gaaatttctt ttcagctgca gattgtgcta gagtagtcaa
     9121 tgcagtggtt aatgcaaaaa gtcctaaaac tatctttttc attttcttct gtgttattta
     9181 tattaatttt aaataaattt catcaaaatg actaaatttg aagcaaaatt atacaaatta
     9241 atgaaattca tctttttttt tatagttagt ctaataattt taacaacctt ctcttgcaag
     9301 agaaacccat tggatgttga cacaagtaat gtaaagttag aaattcaata tgtcaacttg
     9361 gattcattgt tattcaacac accaacagat aaattaattg acttgcgcca acagatggga
     9421 caaaaaatcc ctgaattatt tgactatcaa attggatatt gcatgcgaat tgggaaagtg
     9481 gttgacacgg cttttgtgaa cagtattgtg caataccgac aagataccat gattcaaaaa
     9541 ttggaaaaag aaattgctca taagtttagt aatctaagtg ctcaaaaaag caatttaaca
     9601 acggctttca aacggttaaa agcacatatt aaaactgcaa atgtacctac ctcaatagta
     9661 tttcaaaatt cgttatttac cagcagtgct ttttgtacag agcaagaaat tggaattggg
     9721 ttggaacgct atttaggtgc acaatcgccc attattaaaa gtttgccagg cgaacctttt
     9781 tacgactgga ttaaacaagg ttatgatgtt caatttctag aacgcgatgc tgttttatcg
     9841 tggattttaa ctcacattat tccagaaata aaaggaaatt tagcagaatt gatgatttat
     9901 cacggtaaag cattgtacct caccgaagcg tgcttgcctg agagtgaaaa aaacatcatt
     9961 attcgttatt caaaagaaga tttgaaatgg tcgcaagaaa atgaatacag tttgtggaaa
    10021 taccttgtgg atgaaaaaat actgtttaaa acagatgatt taattgccaa aaatatgctg
    10081 caagaaggtc catttacttc tggtctccca gaaaaaggtc cagatagatt aggacaatac
    10141 cttggctggg tgatcgtaaa aaactacatg gaaaattctg aaaccacatt tgaggaatta
    10201 atcaacactc catacaacga gatactacaa tcgtatcaac ccaaataatt atataaaaat
    10261 ttatagcatg agtcaagaag ataaaattgt aaaaacttcc gaaattgttt taaaagtagg
    10321 aatgaacgaa aataatttgc ctttaaaaat taaatggacc gcaaaagacg gtgatgtaga
    10381 agacaaacct gcacgtgcca tgcttttatc ggtttgggat cataatgaaa acaacacgat
    10441 gaaaattgat ttatggacca aagacatgtc ggtggaggaa atgaaacaat ttttccatca
    10501 aacattgctc acaatggcag atacctttga gcgtgcaaca ggtgaacgca acattagcga
    10561 ggatttacgc gattattgct atcactttgc tgataaaatg caaattttac ccgaaaatta
    10621 atgaaattta ttactcgtaa aatgaatgct gtgctcaccg atacggttga gtacagtttt
    10681 cgtattggct ctgataaaat tgtagtaaac gattatatag gaaaacccat tcgattagca
    10741 ttttctggag ctatttattg tgttaactgt tccaaaaaaa tcaacaaatc gtttcaagga
    10801 ttttgtttca attgttttca aacagcagca aatgcagcag aatgcatcat tcatccagaa
    10861 ctttgcaggg cacatttggg cgaaggtcgc gatgtggaat gggaacaagc caaccacaac
    10921 caaccacatg ttgtttattt ggcactttcg agcgagataa aggtaggcgt aacgcgcacg
    10981 actcaggtgc caactcgttg gattgaccaa ggagcaagcg ctgccattcg gttgatggaa
    11041 actcccaatc gctttgaagc tggaagaatt gaagttgccc taaaaagtat ttttaccgat
    11101 aaaaccaatt ggcaaaaaat gctcaaaaat gaaatcaacg aaaccatcga tttagtggaa
    11161 gaaaaatggc gtttgcaaga acaattgccc gatgatttaa ccacctattt ttcggaagat
    11221 gacgaattga tagaaatcaa ctatcccgtt ttgcaatacc ccaaaaaaat caattctctt
    11281 tcgtttgaca agcaagcagt aattgaaaaa acattgattg gcatcaaagg tcaatattta
    11341 cttttcgacg atcaaagtgt gctcaatatt cgcaagcacg aaggttacga aattgaattc
    11401 ttaggttaaa aaagtgctgc aaccttttta ccaacgttgg tacccagcgc cactcctatt
    11461 ccacccaaac gagctcccac tgcacagttg tcgttaattt tttgaatgat cggaaatttg
    11521 cttgtgccca ttcccatagt gcccgcccat tgataatcga tggtgaattg ggtgttggga
    11581 ataatcattg ttttaagcaa ctccaccaaa cggttttgaa tcaatggtgt ggtggtcatt
    11641 tcatacgttg tttcgccttc aaaatcaagg ttgcgtgccc cgcccaataa aattctgttt
    11701 tcaaaatttc taaaataata gtaacccgca tcgtaatgaa acgttccttt tagttgtaaa
    11761 tttgaaatgg gtgatgttac caaaacctgt gctctggcag gatttacatc caattctttc
    11821 accaattttt gtgtaaaacc gttggttgtt ataaccacat tggaagcagt aatttgtccg
    11881 atggtagttt caaccgaatt atagtttgca ccaagtgttt caacttcaat accattgagc
    11941 aacaacactc ctttttctgc aagaagttga taaaaacggt gcatcatttt tcctgtgtct
    12001 atctggcctt cttgtagtat tttaaaagct gttgaaagtt gatgaaatcc aaagcgttga
    12061 attgcttgcg catcttcttc aaatcctttt ttgcccgtta gacggatgaa ttctgtattc
    12121 aattcattca aattatctct cacctccgtt gcaattgccg tttgttgatg gtcaatcaaa
    12181 tcccaaccac cattggcttg aaaatccatt gctgcatcgc caattaattc gcgcagatag
    12241 attaatcctt catagcgagc ttgaatggtt tcccacacaa gtgatgcagg gtgttttttc
    12301 aaatcttcta ccaattcaac gggaccgcca aaacttgcaa aaccagcatt tttagtactt
    12361 gctccagatg gtaaatagcc tcgttccaaa acaacaattt ttgcagttgg aaatttcatt
    12421 tttaaatgat aagctgttga acaaccgaca attccaccac cgattattaa aaaatcgatg
    12481 ccttcaaaat acgtttttcg ttcccaaaaa ctaagttgat tgatgtttag cataatattt
    12541 gattcaaaaa ttcaagactt tttcgatatt tgtcattatt tttaacccaa atttaaacca
    12601 aatctgtaac aagttgtaca gtaaagacgt ttaagtagtt aatatgaaaa aacttttctt
    12661 ttttctcctc ctttttttcc cctttttggg gattagtcag tcgttgaatg taaaatttga
    12721 cggcgcagtt tctaattttg ataaaggaag tagcgaatct ggtgttaccg tgagcattat
    12781 tcaaaatggc caagcagtaa gctcagcaac aaccagttct aacggacgct attctgttaa
    12841 aggagccatt gacattaaaa aaccatttga cgtagttttc aaaaaaagtg gatttgttag
    12901 caaaaaagta agttttaatt ttattggatt aaatttagaa gatacgccag cgggagattt
    12961 taaacccgtt gacaaattgg atttagatat ttttgcggaa cgtccgggat tggatttttc
    13021 atttttaaat acacaaagtg taggagaatt tacctggaac tcacaaggtt atgtagaagt
    13081 aaacgatgct aagcgtaagg ctgttgctga taaaattgat aatttactca agaaatcgga
    13141 acaagacgcc caaaacaatc aagcagctta caatgcagcc attcaagcag ccgacaaagc
    13201 atttaacgcc aaagattata ccgcagcgtt agctaaatat gaagaggcaa caaaatacaa
    13261 acccaaagaa acttatccca ttcagcgcat agatgaaatt gatgcactac ttcaaaagca
    13321 aaaagaagat gccttgaaat ttcaacaaga aaatgcagct tatttgaatt taattaaagc
    13381 ggctgataac ttcttcaaat taggcgatta cgaaaaagca aaacaaaaat actacgaagc
    13441 atccgacatt agcgatgagc aatatccttt ggatcaaatc aaggaaatta accgtttggt
    13501 aaaggaaaaa gaaaacgaag cgaaatacaa acagctcata gaag
//
LOCUS       DAOQVQ010000011        13298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5246_length_13298_cov_4.290191, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000011 DAOQVQ010000000
VERSION     DAOQVQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5246_length_13298_cov_4.290191"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..2110
                     /gene="rnr"
                     /locus_tag="PKN22_00795"
     CDS             35..2110
                     /gene="rnr"
                     /locus_tag="PKN22_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNS41268.1"
                     /translation="MRKIFEKNEDSEFTHHQICTLLDLRDGALRQLAFSVLQDLKEQN
                     VIKAISHNTFKLNQLSTYVEGYLDLNARGAGFVSAEGFDKDIYIDPLSVGKSLGGDKV
                     KVRITRMGKNRVEGEIVDIIERERTHFVGTIQMHDNFAFLVPDNLRAGTDIFIPKEKL
                     NGAKNGEKVLVKITVWPKTAASPYGEVVERLGQRTANDTEMISILVNQCIDYIFPQEV
                     LAQAEQVSMELEEEEIQRRRDMRDVLTFTIDPFDAKDFDDALSFKRLENGHLEIGVHI
                     ADVSHYVQPGSPMDKEALHRSNSVYLVDRVVPMLPEQLSNFACSLRPNEDKYSFSVVF
                     QMTEEGTIKNVWFGKTVIHSDRRFTYEEVQEMFEGKDGDYKEELVLLDKIAKIYRKKR
                     LKSGALNIESEEMRFKLDEDKNPIEVVIKTSKDAHKLIEEFMLLANRHVAEFIGKPKP
                     NRDFIPFVYRIHDEPDAGKIELFKVFIDKFGYQLENADLSKISKSINALLEDIRYKNE
                     YSIIQSMAIRSMAKAEYATDNIGHYGLAFEYYTHFTSPIRRYADLLVHRILLEELTHQ
                     KHKYGSGLNEVCKQISRNERKAADAERESTKYFQTIFVLDKIGEEFDGTVSGITENGM
                     YVKMTENQCEGMIAMNQLPGDRYYFDEEKYRIVGAKTKHEFNLGDKVRVRIYEVSPRK
                     RQIDLELVV"
     gene            complement(2115..2789)
                     /locus_tag="PKN22_00800"
     CDS             complement(2115..2789)
                     /locus_tag="PKN22_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41269.1"
                     /translation="MSRSLKIAIVGDYNFTYYSHQATNLSLDHAAVFLDVDINYYWVR
                     LSEAVTFKPNQFEQYDGVWIAPGPFTNLFYVAGIIKTLVALKIPTLITGEGFKTFVEV
                     LLNNGSVNLDNTKLISENLVTGNKFEKITILPHSDAFIQLFEHHSTYELSSSRYSIYP
                     QAISQLVESNIDIEAYNQFEDPEIISLKNHSFFVASSYCPQITSTRELPHPLVYTFIK
                     ACMLDK"
     gene            complement(2814..3446)
                     /locus_tag="PKN22_00805"
     CDS             complement(2814..3446)
                     /locus_tag="PKN22_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41270.1"
                     /translation="MNYILKSVAVLSLGIAFFSCNKREIIPAPTPKVDLKSHFYGKIN
                     GTNIELTQNVNGYAGTSGVDLIVNANTLDSAIYHSKFSSSNSLQQVNIAHGSIVFDAN
                     ASERPSVSTFESFYLSGPNLVPSFSVNGLGGISFSFTDGSGKEWKSKTIGTAQYTKMG
                     VESDENGDYAKFRVEFDTRVYYSYLDVPTQTTITDSMEVVDAVYTGWYKR"
     gene            complement(3550..3870)
                     /locus_tag="PKN22_00810"
     CDS             complement(3550..3870)
                     /locus_tag="PKN22_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41271.1"
                     /translation="MKAHTTTFSIDTVLYDNSDTKVLIKGKTTKHHLSYSSSMYISFS
                     ELNLLINELQQKNPDVEVASMFVEEQFSSDYIQMLLHASALENNTVELSLFAMDGTKK
                     MIRA"
     gene            3951..4715
                     /locus_tag="PKN22_00815"
     CDS             3951..4715
                     /locus_tag="PKN22_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS41272.1"
                     /translation="MPEFPLFHKLLPFNGKIDNPLVLIHGFLESHTMWYQLPLEQFQR
                     PILLLDVPGFGKSELLDDELPSIRHYAQAIAQLLEHYNFNSFQLVGHSMGGYIGLELL
                     KMTEKAEKLILLNSNFWCDSDAKKKDRVRAASILLQHKDAFIAEAIPNLFLNPTTSSK
                     QIHQLVEEAKKGVAEWYAYASLAMKERNDFSEFLKNNPARFEIIHGKQDGLIPFQLLQ
                     EKCGAWKTPAIIENSGHMSVFEQPQEVMDLLKKLLN"
     gene            complement(4712..5953)
                     /locus_tag="PKN22_00820"
     CDS             complement(4712..5953)
                     /locus_tag="PKN22_00820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNS41273.1"
                     /translation="MYEKQINELETQLFQKDFKLNSLLEITTAINANASVEKLTEIYS
                     FILKEQLGFTRFLLFTKQEDESWKLLVKNGIRGKLLTLNVGEELARFKDITFIHSSTS
                     DVLNEFDIVIPVYHKEEALAYLLMKVNSPIDFYQEDVQNNFGFIETLTNIIVVAIENK
                     IMAKQRVIQERLKKELEVASEMQKLLFPTDLPSNKQMDISAKYIPRHEVGGDYYDFIP
                     LGNDEFILCIGDVSGKGIGAAMLMANFQATLRTLFIYQKYDLPFLVKELNAKVMKNAK
                     GEKFITFFIAHYNAKTRVLKYINAGHNHPVMTTSKKDVTMLTEGTIGLGMLEELPFIN
                     VGEVVLKPNTTIVSYTDGVVELENKEGEQFGIDRLIKTIHSFYALSMDDMNNLIFSKL
                     EEWKGELEYVDDTAIFACRFF"
     gene            complement(5953..6879)
                     /locus_tag="PKN22_00825"
     CDS             complement(5953..6879)
                     /locus_tag="PKN22_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNS41274.1"
                     /translation="MKLDRNYTVKEIAALIGCSFVGDENHVVSGINEIHKVVTGDLVF
                     VDHPKYYDKALNSAATTILIDKEVECPQGKALIVSAAPFDDYNKLTRHFTPIIEQQQL
                     VGINTHIDPTAVIYPNAFIGNNVTIGKNSRIYPGAVILDRTIIGDNVMIGPNTTIGFN
                     AFYYKKKPTGYDRMHTCGWVHIHNNVEIGANCTIDAGVSGNTEIGEGTKIDNMVHIGH
                     DTVVGKDCLFAANVGIAGCVVIKDKVSMWGQVGCAANVVIGEGVSVLAQSGIGKDLEA
                     GKTYFGSPCGEVKTKFREVAALKKLPDLLNRL"
     gene            7110..8858
                     /gene="aspS"
                     /locus_tag="PKN22_00830"
     CDS             7110..8858
                     /gene="aspS"
                     /locus_tag="PKN22_00830"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNS41275.1"
                     /translation="MYRTHTCGELRIENCGQTVTLSGWVQKSRDLGGMTFIDLRDRYG
                     ITQLAFNEVDNAELTAQARKLGREFVVQATGEVIERASKNKNIPTGEIEIKVTAITLL
                     NASKIPPFTIEDDTDGGDELRMQYRYLDLRRNPVLQKLQLRNKVALETRKYLDTQGFM
                     DVETPFLIKSTPEGARDFVVPSRMQEGQFYALPQSPQTFKQLLMVSGIDRYYQITKCF
                     RDEDLRADRQPEFTQIDCEMAFVEQEDVLQTFEGMIKHLFKAVRGVEFEGDFPRMTYA
                     EAMEKYGSDKPDIRFGMEFIDLKAVTSNKGFAIFDDAEAVIGINAQDCSEYTRKQLDA
                     LTDWVKRPQIGAKGLVYVKYNTDGTLKSSVDKFYSQEDLAEWAKLANAQPGDLLLVLS
                     GDLDKVRKQMNELRLHMGNELGLRNPNVFKPLWVLDFPLLEWDEEAQRFFAMHHPFTS
                     PKPEDMHLVATDPGKIRANAYDLAMNGVELGGGSIRIHDRTLQAQMFDLLGFTPEEAQ
                     NQFGFLMNAFEYGAPPHGGLAFGLDRLVATMGGSDSIRDYIAFPKNNAGRDVMIDAPS
                     PLNAEQLKELNIKVGV"
     gene            9009..9671
                     /locus_tag="PKN22_00835"
     CDS             9009..9671
                     /locus_tag="PKN22_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS41276.1"
                     /translation="MVAIDLITEDIPPLTHLDTGEKALRWMDEFRVSHLPVLKNGNFV
                     GLLSESDVLDKMDEKQTLDVLFEHLPRPFVWAGDHLFEVIDKMGEYNITVVPILDKEE
                     KYLGCTCIHQLIRAVSRIGGITEPGGIIVLDVAKANYSLAQIAQIVESNNAKILSSYI
                     YSPSDSSQMDVTIKVNETDLERILRTFYRYDYTIKASYQRTDSEDELKNRFDALMKYM
                     NL"
     gene            9675..10556
                     /locus_tag="PKN22_00840"
     CDS             9675..10556
                     /locus_tag="PKN22_00840"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNS41277.1"
                     /translation="MNVAVFSRKITKANLPYVVELLAHCQQLEWTVVLELELKKQLEK
                     LIKLPHATATFSSHKDFKKGIDLTISLGGDGTFIQTVGYVRNSNVPIIGINTGRLGFL
                     ANVAKESMREALNLLNNKHYELQQRSLLCVETEKQHFGDANFAINEVAVSRKDSSTML
                     TVHAQLDQQYMNSYWGDGLIISTPTGSTAYNLACGGPIIDPQCQLNVITPIAPHNLNV
                     RPIVVPNEKEIRLSVEGRGRNFLLSLDGHSVNIKKHEEVVVRKADFDINVVKLHENEF
                     LDTIRNKLLWGIDRRNK"
     gene            10611..11696
                     /locus_tag="PKN22_00845"
     CDS             10611..11696
                     /locus_tag="PKN22_00845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS41278.1"
                     /translation="MLGLPTFAQKNAKNENLEDGIYAEFNTSKGRILVKLEDQKTPMT
                     VANFVGLAEGNFTVQDTIKFSKPFYDGLKFHRVIADFMIQGGDPQGNGMGGPGYKFYD
                     ETRADLKHDGPGVLSMANSGPATNGSQFFITHKATPWLDGKHTVFGHVVEGQDVVNAI
                     KQDDVMNTVKIIRVGKTAKKFDATATFNSINKKIGEEYAKEAAELEKIAKMSQDEYKT
                     YMYQEVLKKFPTAKQSESGLVYIVEKAGEGLAIEKGAPVSLHYLGTFRRDGKKFDSSY
                     DRKMPMDFQFQVNRMIPGFEEGIAMLKKGGKAKLIIPYYQAYGPNGRPGAIPPYSDLV
                     FDIEIVDVQAPAAVEEPHDHSDPNHRH"
     gene            complement(11919..12004)
                     /locus_tag="PKN22_00850"
     tRNA            complement(11919..12004)
                     /locus_tag="PKN22_00850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11969..11971),aa:Ser,seq:gga)
     gene            complement(12029..13054)
                     /locus_tag="PKN22_00855"
     CDS             complement(12029..13054)
                     /locus_tag="PKN22_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNS41279.1"
                     /translation="MKRKVLIIYTGGTIGMEKDYETNSLRAFDFNHIFNKIPEMNLLE
                     CEVSVHQFSEPLDSSDVGPEEWKEIAQLIEENYHLFDGFLILHGTDTMAYTASALSFM
                     LKNLRKPVILTGSQLPIGDLRTDAKENLLTSLYYASLYQNDEAVVQEVALYFEYKLLR
                     GNRCLKYSAENFDAFISPNYPILGESGVHLKVEEAMLYRTPHQDDFSVDLHLNDSILL
                     WRIFPGINLESLPQIPKIKVLILQVFGSGTIFSNKKTEEVLQKIRNNGTEIVVISQCV
                     SGGISIGKYNNSNIFKKIGAISGHDITAESALAKAMHLIDNPNYSGDFAENFKKNLRG
                     EISEIEE"
     gene            13141..>13298
                     /locus_tag="PKN22_00860"
     CDS             13141..>13298
                     /locus_tag="PKN22_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNS41280.1"
                     /translation="MIPKNRLQETFDYLKQFLTDERLSKIEHFSHESSDFVLPVMEDV
                     YQFRNAAA"
BASE COUNT         4322 a         2455 c         2554 g         3967 t
ORIGIN      
        1 agggccaggt ttttttcttt agcggtgtaa gcgtattcgt aagatttttg aaaaaaatga
       61 agacagcgaa tttacacatc accaaatatg cacactttta gaccttagag atggagcttt
      121 gcgccaattg gctttttctg ttctgcaaga tttgaaagaa caaaacgtta taaaagcaat
      181 tagccacaac acttttaaac tcaatcaact ttctacttat gtggaaggtt atttggattt
      241 gaatgccaga ggagcaggct ttgttagtgc cgaaggtttt gataaagata tttacatcga
      301 tccgctttct gtggggaaat cgttgggagg cgacaaagtg aaagtgcgca tcacccgcat
      361 gggaaaaaac agggtagaag gtgaaattgt ggatatcatt gaacgcgaac gcacccattt
      421 tgtaggaacc attcaaatgc acgataattt tgcgttttta gttccagaca atttacgtgc
      481 gggtaccgat atttttatcc ccaaagaaaa actaaatggt gccaaaaatg gcgaaaaagt
      541 attggtaaaa attaccgttt ggcccaaaac agccgctagt ccgtatggtg aagtagttga
      601 acgattaggg caacgcacag ctaatgacac cgaaatgatt tctattttgg ttaaccagtg
      661 catcgactat atttttccgc aagaagtgtt agcacaagcc gaacaagtga gtatggagtt
      721 ggaagaggag gaaattcaac gtcgtagaga catgcgcgat gtattgactt ttaccatcga
      781 cccttttgat gccaaagatt ttgacgacgc actatcgttc aaacgccttg aaaatgggca
      841 tttggaaatt ggtgtccaca ttgcagatgt tagtcactac gtgcaacctg gttcaccaat
      901 ggataaagaa gcattgcacc gctccaattc ggtttattta gtggataggg tagtgcctat
      961 gttgcccgag cagttgtcta actttgcttg ttcgttgcgc cccaacgaag ataaatacag
     1021 cttttcggta gtgtttcaaa tgactgaaga aggaactatt aaaaatgtat ggtttggaaa
     1081 aacagtcatt cactccgaca gacgctttac ctatgaggaa gtacaagaaa tgtttgaagg
     1141 caaggatggc gactacaaag aggaattggt gttgctggat aaaatcgcaa aaatttatag
     1201 aaaaaaacga ttaaaatcgg gcgcgctcaa cattgaatct gaagaaatgc gctttaagtt
     1261 ggacgaagac aaaaatccta ttgaagtagt tatcaaaact tcaaaagacg cgcataaatt
     1321 aattgaagaa ttcatgttgt tggccaaccg acacgtggca gaatttatag gtaaaccaaa
     1381 acctaatcgc gattttattc cgtttgtgta ccgtattcac gatgaacctg acgccggaaa
     1441 aatagaattg tttaaggtgt ttattgataa gtttggctat caattggaaa atgcagattt
     1501 aagcaaaata tcaaaaagca tcaacgcctt gttggaagat atccgctata aaaacgaata
     1561 ttccattatt caatccatgg caattcgttc catggcaaaa gcagaatatg caaccgacaa
     1621 tataggccac tacgggttgg cttttgagta ctacacccac ttcacgtcgc caattcgccg
     1681 ttatgccgat ttattggtgc accgcatttt gttggaagaa ttaacgcacc aaaaacacaa
     1741 atacggaagt ggtttaaacg aagtgtgtaa acaaatttct agaaacgaac gcaaggcagc
     1801 agatgcagaa cgagaatcaa ccaagtattt tcaaaccatt tttgtgttag acaaaatagg
     1861 agaggagttt gatggcactg tttctggaat tactgagaat ggaatgtatg ttaaaatgac
     1921 cgaaaaccag tgtgagggga tgatagcaat gaatcaatta cctggcgata ggtattattt
     1981 tgacgaagaa aagtatcgaa ttgttggtgc aaaaaccaag catgaattca atttaggcga
     2041 caaagttcgt gtacgcatct acgaagtttc gccacgtaaa cgtcaaattg atttggaatt
     2101 ggtggtttaa ccaactattt atccaacata catgctttaa taaaagtata aaccaacgga
     2161 tgaggtaact cgcgtgtgga agtaatttgt ggacaataac tacttgccac aaaaaatgaa
     2221 tggtttttta aactgataat ttctggatct tcaaattggt tatacgcttc aatatcaatg
     2281 ttcgattcaa ccaattgtga gatggcttgc ggataaatac tataacgcga agacgacagc
     2341 tcataggttg aatggtgttc aaacaattga ataaacgcat cggaatgagg caaaatagta
     2401 attttttcaa atttattgcc tgtaactaag ttctccgaaa tcaatttggt gttatcgagg
     2461 ttaacagaac cattattgag caatacctcc acaaaagttt taaatccttc gccagtaatg
     2521 agagttggaa ttttcaaagc aaccaaagtt ttgataattc cagcaacata aaacaagttg
     2581 gtaaatggcc caggagcaat ccaaacacca tcatattgtt caaattgatt aggtttaaat
     2641 gttacagcct cacttaatcg cacccaataa taattaatat ccacgtccaa aaacaccgct
     2701 gcatgatcaa gcgataaatt ggtggcttgg tgagaataat aggtgaaatt ataatcgcca
     2761 acaattgcta ttttgaggga gcgactcatt cgaatcagcc gagaaatgca ctattatctt
     2821 ttgtaccaac ctgtataaac cgcatcaacc acttccatag agtcagtaat tgttgtttgt
     2881 gttggtacat ctaagtaaga ataataaacg cgagtgtcaa attctacacg aaatttagca
     2941 tagtcaccat tttcatccga ttcaacaccc attttagtgt attgtgctgt tccaattgtt
     3001 tttgatttcc attcttttcc tgaaccatct gtaaaagaaa aagaaattcc gcctaaaccg
     3061 ttaactgaaa aacttggaac taagttcggt cccgacaaat aaaaactttc aaacgtagaa
     3121 actgatggac gctctgacgc attggcatca aatactatgc ttccatgagc tatgttgact
     3181 tgttgtaaag aatttgatga agaaaattta gaatgataaa ttgcactatc caacgtgtta
     3241 gcatttacaa ttaaatccac acctgatgtg ccggcatatc catttacatt ttgagttaac
     3301 tcaatatttg taccgttgat tttaccataa aaatggcttt ttaaatcaac ttttggagtt
     3361 ggagcaggga taatttctcg tttgttacaa gaaaagaaag ctattccaag agataaaaca
     3421 gctaccgatt ttaaaatata attcatcgta ctaagtttta attttcacaa taatctagcc
     3481 tgcaaaaagg gtagagattc agtaaaaata cgaattattt taaaaaaagt tgcgtttagt
     3541 taaaaattct taagcgcgaa tcattttttt ggtgccgtcc attgcaaata aactcaactc
     3601 aacagtgttg ttttccaacg ccgatgcatg caataacatt tggatataat ccgatgaaaa
     3661 ttgctcttca acaaacatag aggcaacttc tacatcagga tttttttgct gtaattcatt
     3721 gattaacaaa ttcaactccg aaaacgaaat atacatggaa gaagaatacg acaaatgatg
     3781 cttggtggtt ttgcctttta ttaatacttt cgtatcgcta ttgtcataaa gaacagtatc
     3841 aatgctaaat gttgttgtat gtgctttcat ggtatttgat ttttcaatta acactgcaaa
     3901 gttccgatgc aactacgtaa gttttttacg ttctaaataa tttttaaaaa atgcccgaat
     3961 ttcccctgtt tcataaattg ttgccgttta atggaaaaat agacaatcct ttggtattga
     4021 ttcacggttt tttggaaagc catacaatgt ggtaccagtt acctttagag caatttcaac
     4081 gacccatcct tttgcttgat gtgcctggtt ttggaaaaag tgagttgttg gacgatgaat
     4141 tgccttccat ccgccattat gcgcaagcta tagcacaact gttggagcac tacaatttta
     4201 attcgttcca attggtggga cactcaatgg gtggctacat aggattggaa ttactaaaaa
     4261 tgaccgagaa agcagaaaaa ttaattttgt taaactctaa tttttggtgc gattcggatg
     4321 caaagaaaaa agaccgtgtg cgtgcggcgt ctattttact tcaacacaag gatgcgttta
     4381 ttgcagaggc aattcccaac ttgtttttaa acccaacaac atcctcaaaa caaattcacc
     4441 aattggtgga ggaagctaaa aaaggagtgg cagaatggta tgcctatgca tcgttggcaa
     4501 tgaaagaaag aaatgatttt tctgaatttt taaaaaataa tccagctcgc tttgaaatta
     4561 ttcatggcaa acaagatggg ttgattccct ttcaattatt gcaagaaaaa tgcggagcgt
     4621 ggaaaacacc agcaattatt gaaaattcag gccacatgtc ggtttttgaa caaccacaag
     4681 aagtaatgga tttattaaag aaattgttga attaaaaaaa gcgacaagca aaaatcgccg
     4741 tatcgtccac atattccaac tcgcctttcc attcttcgag ttttgaaaaa atgaggttgt
     4801 tcatatcgtc catgctcaac gcataaaacg aatgaatggt ttttatcaaa cggtcgattc
     4861 caaattgctc tccttcttta ttttccaatt ccaccacacc atcggtataa gaaacaatgg
     4921 ttgtattggg ttttagcacc acttcaccca cattgataaa gggcaattct tcaagcattc
     4981 ccaaacctat ggttccttcg gttagcatgg ttacgtcttt ttttgaagtc gtcatcaccg
     5041 ggtgattatg ccctgcattg atgtatttca acacacgcgt tttggcgttg tagtgggcaa
     5101 taaaaaaggt aatgaatttt tcgccttttg catttttcat cactttggcg ttcagttcct
     5161 tcactaaaaa cggcaaatcg tatttttgat aaatgaacag cgttcgaagc gtagcttgaa
     5221 aatttgccat gagcattgca gctcctattc cttttccaga aacatcgcca atgcacaaaa
     5281 taaattcatc gtttccgagt ggaataaaat cgtagtaatc gccacccacc tcgtgtcgtg
     5341 gaatgtattt ggccgaaatg tccatttgct tgttggaagg taaatccgta ggaaacaaca
     5401 atttttgcat ctcgctggct acctccaatt cttttttcaa ccgctcttga atcacccgtt
     5461 gtttggccat gattttattt tcaattgcta ccacaataat attggtgagc gtttcaataa
     5521 aaccaaagtt gttttgcaca tcttcttggt aaaaatcaat aggtgagttg actttcatca
     5581 gcaaataagc caatgcctct tctttgtgat ataccggaat tacaatgtca aattcattca
     5641 acacatcaga agtagaggaa tgaataaagg taatgtcctt aaatcgcgcc aattcttcac
     5701 ccacattcag ggtcaataat tttccgcgaa taccgttttt taccagcaat ttccaacttt
     5761 catcttcttg cttggtaaac aataaaaagc gagtaaaacc caattgttct ttcaaaataa
     5821 acgaatagat ttctgtgagc ttctccacac tcgcattggc attaattgcc gttgtgattt
     5881 ctaagagaga attcagctta aaatcttttt gaaacagttg tgtttcaagc tcatttattt
     5941 gtttttcata cattataaac ggttcaataa atcgggtaat tttttcaatg ccgctacctc
     6001 tctaaatttt gtttttactt ctccacacgg actaccaaaa taggtttttc cagcttccaa
     6061 atcttttcca atgcccgatt gtgccaacac cgacacacct tcgccgataa ccacattggc
     6121 agcacaacca acttgtcccc acatagaaac tttgtcttta atcaccacac aacctgctat
     6181 accaacattg gcagcaaaca aacaatcttt gccaacaacc gtatcgtgac caatgtgcac
     6241 catgttgtca atttttgtgc cttcaccaat ttctgtattg cccgatacac cagcgtctat
     6301 cgtacaattg gcaccaattt ccacattgtt gtgtatgtgc acccacccac aggtatgcat
     6361 gcgatcatat cccgttggtt ttttcttgta ataaaatgcg ttgaatccaa tagtggtatt
     6421 aggtccaatc attacattat caccaataat ggtacggtct aaaataacag cacctggata
     6481 aatgcgtgag ttttttccaa tcgttacgtt gttgccaata aatgcgttag gataaataac
     6541 agctgtagga tcgatgtgtg tattgattcc cactaattgt tgttgctcaa taataggcgt
     6601 aaaatggcgg gttaatttat tgtaatcgtc aaacggagcg gcagaaacta tcaatgcttt
     6661 accttgcgga cattctactt ctttgtcaat caagattgtt gtggcagcac tgttaagcgc
     6721 tttgtcgtaa tatttagggt gatccacaaa gactaaatca ccggttacaa ctttatgtat
     6781 ctcattaatt ccagatacaa cgtggttttc atctccaaca aacgaacaac caatcaacgc
     6841 agcgatttct ttcaccgtat aatttctgtc tagcttcata caaaatgtgt tataaaacaa
     6901 aattactgct aattctacac actaaaaaaa cgcaagcaga atgttttccg ctaacaattg
     6961 ttaaaagcga aatgaattaa attagaacat agaatttcag gcaaatagtt tggttttttg
     7021 agtcacttct attcacctct caacaatatg tttcctaaaa ttcaaaaaat acagttgata
     7081 tttataattt tgcaacaaaa taaaaaaaca tgtacagaac acatacttgc ggagaattaa
     7141 gaattgaaaa ttgtggtcaa acagttacac tttcaggatg ggtgcaaaaa tcgcgcgact
     7201 tgggaggaat gacatttatc gatttgcgcg atagatacgg cattacacag ttagctttta
     7261 acgaggtgga caatgcagaa ttgactgcac aagcacgtaa attgggacgt gagtttgtgg
     7321 ttcaagctac aggtgaagta attgaaagag caagtaaaaa caaaaatatt ccaaccggtg
     7381 aaattgaaat taaagtaaca gcaatcacct tattgaatgc ttcaaaaata ccaccgttta
     7441 ccattgaaga cgatacagat gggggcgatg agttgagaat gcaataccgt tatttggatt
     7501 tgagaagaaa tccggtgttg caaaaattgc aattgcgcaa taaagttgcc ttggaaacca
     7561 gaaaatactt ggatacacaa ggatttatgg atgttgaaac accattttta attaaatcaa
     7621 ctccagaagg agcacgcgat tttgtggtgc ctagccgcat gcaagaaggt cagttttatg
     7681 ccttgcctca atcacctcaa acgtttaaac aattgttgat ggtatctgga atagaccgtt
     7741 actatcaaat tacaaaatgt tttcgcgatg aagacttacg tgctgatcgt cagccagagt
     7801 ttacacaaat tgactgtgaa atggcgtttg tagaacaaga agatgtgttg caaacatttg
     7861 aaggaatgat aaagcatttg ttcaaagccg ttcgtggtgt tgaatttgaa ggcgattttc
     7921 cacgcatgac ttatgccgaa gcaatggaaa aatatggttc cgacaaacca gatattcgtt
     7981 ttggaatgga attcatcgac ctaaaagcgg taacaagcaa taaaggtttt gccatttttg
     8041 acgatgctga agcggtgatt ggaatcaatg cacaagattg ttcagaatat acacgcaaac
     8101 aattggatgc gttgactgat tgggttaaac gtccgcaaat tggtgcaaaa ggattggtat
     8161 atgtgaaata caacaccgac ggaacattga aatcttcggt tgataaattc tattcgcaag
     8221 aagatttggc agaatgggct aaattggcaa atgcacaacc aggcgacttg cttttggtat
     8281 taagcggcga cttggataaa gtgcgcaaac aaatgaacga attgcgccta cacatgggta
     8341 acgaattagg attacgcaat ccaaacgtgt ttaaaccatt gtgggtattg gatttcccat
     8401 tgttggaatg ggatgaagag gcacaacgtt tctttgcaat gcaccacccg ttcacttcgc
     8461 caaaaccaga agatatgcac ttagtggcaa ccgacccagg taaaattaga gcaaacgctt
     8521 atgacttggc aatgaatgga gtagagttgg gaggaggttc tattcgtatt cacgaccgca
     8581 ctctgcaagc acaaatgttt gatttgttag gatttacacc agaagaagcc caaaaccaat
     8641 ttggattctt gatgaatgca tttgaatacg gtgctccacc acacggaggt ttggcttttg
     8701 gtttggatcg tttggtagca acaatgggcg gatcggattc tattcgcgat tacattgctt
     8761 tccctaaaaa caacgcagga agagacgtta tgattgacgc tccatcgcca cttaatgcag
     8821 aacaattaaa agaattaaac atcaaagtag gcgtataatt ttttattatt ggtcggtttt
     8881 tatcatcacc ccacaaggta gatgaacaac ttttttgtgc tgtaatgaag gagtcatttt
     8941 caatgaattt gtaaattttt gttatttcat aagctgagtt tgaactattt atttattttc
     9001 gtagtattat ggtagcaatt gacttaatta cagaagatat tccgccactt acccacttgg
     9061 atacaggtga aaaagcgttg agatggatgg acgaatttcg ggtttcacat ttaccagtgt
     9121 tgaaaaacgg aaatttcgta ggcttgcttt ctgaaagtga tgtgttggat aaaatggacg
     9181 aaaaacaaac gttggatgtg ttgtttgaac atttgccacg tccgtttgtt tgggcaggcg
     9241 accatctttt tgaagtcata gataaaatgg gagaatacaa cattaccgtt gtacctattc
     9301 tcgataaaga agaaaaatat ttgggatgca cttgtattca tcaacttatt cgtgccgtta
     9361 gtagaattgg aggcatcacc gaaccagggg gcatcattgt gttggatgtg gcaaaagcca
     9421 attattcact agcgcaaatt gcacaaattg tggaaagtaa taacgcaaaa atcctaagtt
     9481 cttacattta ttcaccctca gattccagcc aaatggatgt aacaattaaa gtcaacgaaa
     9541 cagatttaga acgcatactt cgcacttttt atcgatacga ttacaccatc aaagcatcct
     9601 atcaacgcac cgattctgaa gatgaactta aaaataggtt tgatgcgctg atgaaataca
     9661 tgaatttata gcctatgaat gttgctgtct ttagccgaaa aataaccaaa gcaaatcttc
     9721 catacgtagt ggagttgctt gcgcattgcc agcaattgga atggaccgtg gtattggaac
     9781 ttgaattaaa aaaacaactc gaaaagttaa taaaacttcc gcacgcaaca gcaacgtttt
     9841 cgtcacacaa agattttaaa aaaggaatag atttaaccat tagtttgggt ggcgacggta
     9901 cgtttattca aaccgtaggt tatgtgcgca attccaacgt tccaattatt ggaatcaaca
     9961 caggaagatt agggtttttg gccaatgtgg caaaagaatc catgcgcgaa gcgttgaatt
    10021 tattgaacaa caagcattat gaacttcaac aacgctcttt gctttgcgtt gaaacagaaa
    10081 aacaacattt tggcgatgct aattttgcta ttaacgaggt tgctgtttct agaaaagaca
    10141 gttcaaccat gttgacggtg catgcacaac tcgaccaaca atacatgaat tcatactggg
    10201 gagatggttt aattatctcc acaccaacag gttctacagc ctataactta gcttgtggcg
    10261 gaccgattat tgaccctcaa tgccaactta atgttattac gcctattgcg ccgcataatt
    10321 taaacgtgcg accaattgtt gttcccaacg aaaaagaaat tcgtttgtcg gttgagggca
    10381 gaggtagaaa ttttttattg agtttggatg gccactcagt aaacattaaa aaacacgaag
    10441 aagtggtggt gcgcaaagcc gattttgata taaatgttgt aaaactccac gaaaatgaat
    10501 ttttagacac cattcgcaac aagttgttgt ggggaattga tagaagaaat aaataaacaa
    10561 aataaacaat gagaaaactt aaaacaatag gaatagttgc ttttgcattg atgttgggac
    10621 ttccaacttt tgcacaaaaa aacgcaaaaa atgaaaattt agaagacgga atctatgccg
    10681 aatttaacac cagtaaaggc cgaattttgg taaaattgga agaccaaaaa acaccaatga
    10741 cggttgcaaa ttttgttggt ttagccgaag gaaattttac tgttcaagac accattaaat
    10801 tttcaaaacc attttatgac ggcttgaaat ttcatcgtgt aattgccgat tttatgattc
    10861 aaggtggcga cccacaagga aacggaatgg gtggtccggg atataaattc tacgatgaaa
    10921 ctcgcgccga tttaaaacac gatggtccgg gcgtattgtc aatggcaaat tcaggaccag
    10981 caaccaacgg aagtcagttt tttattaccc ataaagcaac gccttggttg gacggaaaac
    11041 acactgtttt tggtcatgtt gttgaaggac aagatgttgt caacgctata aaacaagacg
    11101 atgtgatgaa caccgtaaaa atcattcgtg ttggtaaaac agctaaaaaa tttgatgcaa
    11161 cagcaacatt taattcaatc aacaaaaaaa taggggaaga atatgccaaa gaggccgctg
    11221 agttggagaa aattgctaaa atgtcgcaag atgaatataa aacctacatg tatcaagagg
    11281 tgttgaaaaa attcccgacg gcaaaacaaa gtgaatctgg tttggtttac atcgttgaaa
    11341 aagcaggaga ggggttggct atcgaaaaag gagcgccagt ttcattacat tatttaggaa
    11401 ctttccgtcg cgatggtaaa aaattcgatt cttcgtatga tcgtaaaatg ccaatggact
    11461 ttcagtttca agtaaaccga atgattcctg gatttgaaga aggaattgcc atgttgaaaa
    11521 aaggtggcaa ggcaaaatta atcattcctt actaccaagc ctatggacca aacggtcgcc
    11581 caggagctat cccaccttat tctgacttgg tttttgacat tgaaattgtt gatgttcaag
    11641 cgccagcagc agtggaagaa ccacatgatc acagcgatcc caaccacaga cattaataaa
    11701 ttttaaatgg aattaacctc agtaataatc tattgctgag gtttttttat attcagttgt
    11761 ttatagaaag tccttggcaa aaaagataga taatgtgtgc attttttgtt cattttctta
    11821 ggtcttcgct tcgctacggc acgaacccga tggttctcat ccctcacttt tataccaatt
    11881 tgaaataaaa aaggaaccta aacagattcc ttcactagcg gagagagagg gattcgaacc
    11941 ctcgatacag ttacccgtat actacctttc cagggtagct ccttcaacca ctcggacacc
    12001 tttctttttc agatggcaaa ggtagaattt attcttcaat ttccgaaatt tctccacgca
    12061 gatttttctt aaaattttca gcaaaatcac ctgagtaatt tggattatca attaaatgca
    12121 tggcttttgc caaggcactt tcggcagtga tgtcatgacc gcttattgcg ccaatctttt
    12181 tgaaaatgtt actgttattg tattttccga tgcttattcc accagaaaca cattgagaaa
    12241 tcaccacgat ttcagttccg ttatttctga ttttttgaag cacttcttcc gtttttttat
    12301 tgctaaaaat tgttccagaa ccgaaaactt gaagaattaa aaccttgatt ttaggaattt
    12361 gaggcaaact ttctaaattg attccaggaa aaattctcca caaaagaatg ctgtcattca
    12421 aatgtaaatc tacagaaaaa tcatcttgat gaggcgttct gtacagcatg gcttcttcca
    12481 ccttcaagtg aacaccagat tctcctaaaa tagggtagtt tggactgata aatgcatcaa
    12541 aattctcagc agaatatttt aaacaacggt ttcctctcaa taatttatat tcgaaataga
    12601 gcgccacttc ttgtaccacc gcttcatcat tttgatataa actcgcgtaa taaagactgg
    12661 taagcaaatt ttctttggca tcagttctta aatctccaat tggcaattgc gaacccgtca
    12721 aaatcacagg ttttcttaaa tttttgagca tgaaactcag tgcagaagca gtataagcca
    12781 tcgtatctgt tccgtgaaga atcaaaaatc catcaaaaag gtgataattt tcctcaatca
    12841 gttgagcgat ttccttccat tcttctggtc ctacatctga tgaatccaaa ggttctgaaa
    12901 actgatgaac gcttacttcg cattccagca gattcatctc tgggatttta ttgaaaatat
    12961 gattgaaatc aaaagcgcga agactattgg tttcataatc tttctccatt ccgatggttc
    13021 cgcctgtgta tatgatgagg acttttcttt tcatgtaaaa ttttcttttt tgtagaattt
    13081 ttcagacttt aaagttaaaa atttaaagtt aatttctaaa atattttgca attttgccaa
    13141 atgattccta aaaacagact tcaagaaact tttgattatc taaaacaatt tcttaccgat
    13201 gaaagattga gcaaaataga gcatttttct catgaaagtt cagattttgt gcttcccgtg
    13261 atggaagatg tgtatcagtt ccgtaatgca gcagcgat
//
LOCUS       DAOQVQ010000012        13105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5347_length_13105_cov_3.924138, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000012 DAOQVQ010000000
VERSION     DAOQVQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5347_length_13105_cov_3.924138"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKN22_00865"
     CDS             <1..699
                     /locus_tag="PKN22_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNS41281.1"
                     /translation="LIFKDNFSTAAIIFALAVALLFVGKYPFSRIFAMGGIIVGLGLL
                     AVGVQKALPELNILPRLETWENRIFNRIAAENDVVENAQVVNAQLAIYNGGVFGQGVG
                     DGKLKEYLPEAYADFYYSSFVEEFGFIAAIFLVFIYLVLLFRIFRIALKSDNLFETYL
                     CVGLGMLFLTQASVNMLVCTGVFPVTGQNMPLLAMGGSAMIVTCTSLGMIQSIAVKQS
                     KKSKEDNSDNNDEA"
     gene            700..1794
                     /gene="murG"
                     /locus_tag="PKN22_00870"
     CDS             700..1794
                     /gene="murG"
                     /locus_tag="PKN22_00870"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNS41282.1"
                     /translation="MKKVIISGGGTGGHIFPAVAIADELKRQFPSIELLFIGAEGKME
                     MEKVPQAGYKIIGLPISGLQRKITLQNLALPFKIIKSLLLARKVIKNFNPEIVIGVGG
                     YASGPTLKMAALLGIPTLVQEQNSFAGKTNMLLAKKAHKLCVAYEGMEKFFPKNKIVL
                     TGNPVRFEVTQIEGKREAAIAHYQLDASKPVLLVIGGSLGAKTLNESIIAGIEQFQQH
                     GIQLVWQCGKLYFDDLTNRLKGKDITGIHLTQFIAKMDLAYAAADVIISRAGAISVSE
                     LCLVHKPVILVPSPNVAEDHQTHNAMALVNKNAAILVKDSEARNQLVDVAIDLLNNKE
                     KQQTLAQNIAQLGKPNATKDIVAEIVKIVK"
     gene            1798..3183
                     /gene="murC"
                     /locus_tag="PKN22_00875"
     CDS             1798..3183
                     /gene="murC"
                     /locus_tag="PKN22_00875"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNS41283.1"
                     /translation="MEIRKLNIDLKQFENIYFLGIGGIGMSALARYFKALGKNVAGYD
                     KTPSQLTESLVQEGIEVHFEDRNSSIISPYCDKAKTLVIYTPAIPKNHGELMHFQHQE
                     FVLVKRSEVLGLITQNSLGLGVAGTHGKTTTSTMLAHIINQTEEGCTAFLGGISSNYN
                     SNLLLKPTSRYTVIESDEFDRSFLRLSPFSSIITSADPDHLDIYGDAAHFREGFEMYA
                     NLHPENGIVVQKFGLNLPTKAKVYTYGIEQKDADFNGVNLRYVDEQFWMDVVTPFGFF
                     EAIELGVPGSHNAENAVACIALCLELGVEMETIRKGLKSFLGVKRRFEYHIKSQKLIY
                     IDDYAHHPTELKALIDSIKLIYPNRKITGVFQPHLFTRTRDFFDGFAEQLSRFDEAIL
                     MPIYPAREEPIAGVTSDALLDKVHAPAKQLLASQAVIDYLSTIEEGVILTVGAGDIDR
                     IVEPLTKVLSE"
     gene            3176..3973
                     /locus_tag="PKN22_00880"
     CDS             3176..3973
                     /locus_tag="PKN22_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41284.1"
                     /translation="MNKWIKITLWSIVAIAVVVGVVFAQKAINNMTLLAPTIEISVDG
                     ENAILNKEEVMTRLQRQNLIYVNQRVKDLNTERIERFISNMEEVKNVKIYSSFGGKWT
                     ISIVLRKPLFHVYNPSGENYYVDTEGYKISSTIDHAARVLIITADKPLKFNQKTVFEI
                     INNDSLKTIQKIDNVYRISNYVCNDPLMRSLIGQIHLKNNGDFVLIPVVGDQTIVFGM
                     ANTEKEIENKFEKLKIFYKEAIPYEGWSTYSEISLKYDKQIVCKKKE"
     gene            3982..5328
                     /gene="ftsA"
                     /locus_tag="PKN22_00885"
     CDS             3982..5328
                     /gene="ftsA"
                     /locus_tag="PKN22_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNS41285.1"
                     /translation="MSKVVVGLDIGTTKIACFVGQRNEYGKIEILAIGQKESLGVNRG
                     VVENIEKTIQSIKGAIEDAKEYIEQQKDGVEITLRSVYVGVAGQHIKSMQHRGMLVRT
                     NVENEISQKDLDILEDDMYRMLMPPGEQIIKVIPKQYDIDGHQGIKDPIGHAGVKIEA
                     NFHMITGNVGACTNIVRCVTRAGYHVQDMVLEPMASAEAVLSEEEKEAGVVLVDIGGG
                     TTDIAIFHDGIIQHTAIIPFGGNVITQDIKEGCQIMQKQAERLKVKFGSAIVNENQSN
                     EIVCIPGLRGREPKEITVSSLAGIINARMTEIIELIDYEIINTGLKKKLIGGIVVTGG
                     GSQLKHLKQLFEFHTGMDTRIGYPTEHLANNNEIENIESPIYSTGIGLVLTGFKELDK
                     REGYAINANAVNAEIKNDKEEKKVEEPKRIKERKGFFESLISKSKDIGRDFFMDEE"
     gene            5371..7026
                     /gene="ftsZ"
                     /locus_tag="PKN22_00890"
     CDS             5371..7026
                     /gene="ftsZ"
                     /locus_tag="PKN22_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNS41286.1"
                     /translation="MEFELPKDNASIIKVIGVGGGGSNAVNHMYNQGIIGVDFIVCNT
                     DKQALDISPVPYKIQLGPSLTEGRGAGSIPEIGKNAAVENIEEIQNLLSNNTKMVFIT
                     AGMGGGTGTGAAPVIASVAKDMGILTVGIVTVPFGFEGKKRRQQAEEGLDELRKNVDA
                     LLIINNERLRELTGNLSLSNAFAQADDVLAVAAKGIAEVISVTGIINTDLNDVNTVMR
                     NSGVAIMGSAAAEGENRSIVAVQNALASPLLNDNDISGAKYVLLNITYGDAEITMDEM
                     AEVTDYIQEAAGSTAEIIFGHGYDASLGNKLSVTVIATGFASSPITGFEKAPEQIKKV
                     LDEEPKNEIKMPMTSPTESAVWSGAKEVTAEQEEPFIKQVVETKVEDKPQFVSMPTEP
                     VAEVKKVNLDDEVTEQQGAINFDWEVSSPAPIAETPSIPVAENTTSEFVRYTLDEEDE
                     TMNTTDNVESTEKRHVLSPEEQLRKQQERMSRIHEYTTRLKKADGVIELEKEPAFTRR
                     NITIDNSNNYSSSSESSRFGVNSDENGGYRLNGGNSFLHDNVD"
     gene            7032..7478
                     /locus_tag="PKN22_00895"
     CDS             7032..7478
                     /locus_tag="PKN22_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNS41287.1"
                     /translation="MSFTDKINADIKTAMLAKDKERLAALRDIKSKLMLEATSGAGEV
                     TEEAAAKIVLKLHKQRIDTYNLYIEQGRQDLADDELFQAKVIEEYLPKQLSEDEIRNE
                     VKAAIQAVGASGPADMGKVMGVLTKQLAGKADGKIISAMVKEELAK"
     gene            complement(7548..8459)
                     /locus_tag="PKN22_00900"
     CDS             complement(7548..8459)
                     /locus_tag="PKN22_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41288.1"
                     /translation="MLPLHEITNTRILYSFLNWGKGHLSRSIGICKQLVQQGNRLVIA
                     CEKEDFLVLAEYLDDLEFVELANYPFHFSGKGNFAVDLWKSRKALLQHLQNEHVLVEN
                     IVKEYQIDVVLSDHRYGFYSKHIPSVFITHQVNLALKWWQFPAQMIHNRWMQAFNWIW
                     ILDDTTQSLAGKLSANNRWANSSYIGHFSRFDTTTIDTPKKLVIGVCNGPFPYNFQLL
                     QQLEAIKHLDYIITSLPSKDKRAIQPRSWKESDVLFYQAKTIYSYCGYTTLMDLKALG
                     CEGNLYPTPGQSEQVYLHRLHKSKIER"
     gene            complement(8469..9338)
                     /locus_tag="PKN22_00905"
     CDS             complement(8469..9338)
                     /locus_tag="PKN22_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNS41289.1"
                     /translation="MKFILVSLLVGFSFLTFSQNKPAYVIYNAKGKKVSYKKMLQAAI
                     ASDITFFGEFHDNTIAHWLELELTMDMARLTNNQLTLGFEMFEQDQQQLLSDYQSGKI
                     TAKQFEDSTRLWNNYKTDYKPIVNFAKDNNLICVASNIPRWIASLVYKKGRVALDSLK
                     PIDYNHMCDKNYVVDTSLSQYKKMLGMMPDHNKGLNFIYSQASKDATMAHFIDKYWKN
                     YPTNKFIHFNGSFHTDFDQGIIWYLKRLRPNLHYTTITVVEQSDFKKMDKEHIDKAHF
                     IICVDEHMTKTIH"
     gene            complement(9637..10407)
                     /locus_tag="PKN22_00910"
     CDS             complement(9637..10407)
                     /locus_tag="PKN22_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNS41290.1"
                     /translation="MNNSLKKHILDNWKKVGHYKTLGDYEREISIQKKLMELMQVGDF
                     YYFIFAPPMNKIEFVSAEMETILGYKPSEFTVEKLLSIIHPDDLPYFIDFENTVIDFK
                     MKLPPDKVMKYKSRYNYRIRKSNGEYMHILQQSTTIQTDEEGAILRNFAIHTDISHLK
                     RKSDTKMELSFIGLDGEQSFINVSPQKEFIELAAVLTPREMEIVQLIATNLTSKEIAK
                     QLQISPETVNTHRKNIHIKTGTKSAIELVLFAQDKGWI"
     gene            10518..11702
                     /locus_tag="PKN22_00915"
     CDS             10518..11702
                     /locus_tag="PKN22_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNS41291.1"
                     /translation="MSEIKTHIVQRSHELFEKVKAYREHLHQYPELSYQEHQTMKFVA
                     DKLTELGVEHQTGVGGTGVVGIIKASHHTNDMEVVALRADMDALPIHEENNVPYKSKV
                     DGVMHACGHDAHTAILLGTAEILQEIKEQLPQPVKLIFQPGEEKNPGGASLMIKDGVL
                     QNPTVKTIYGLHVFPDLPAGMTGFKGGLYMASCDEIYITIHGKGGHGATPHQCIDPIT
                     IGAELILNLQTIISRRRDPKIPGVLTFGYFEALGATNIIPAKAVLKGTFRTMNEEWRA
                     EALELLQQQAKAIVEGNGATLELEISKGYPYLENDETLTAQLKQKSIDLLGSDNVVDL
                     PIRMGSEDFSYYAQEIPACFFRIGVRDEKNGIIFGAHHARFDIDPQALKIGMQMMSLA
                     VF"
     gene            11706..12266
                     /locus_tag="PKN22_00920"
     CDS             11706..12266
                     /locus_tag="PKN22_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNS41292.1"
                     /translation="MKIFSFCFVFLFVVSCANNDAQEVANTNIIQIQDIGSLATTEYT
                     FSKIVQLNDDKDWYKIGNRKILFSVKAKVKAGIDFNKVQPEDVTIDGKKSIHIKLPEA
                     EILAFEINPDDVQTEMEDISGLRFEFSQQEKLKILKLGEKSIKEDMGKSAILQVAKKN
                     THAFLVDFYTNLGYESVTIETKSAKL"
     gene            12263..12799
                     /locus_tag="PKN22_00925"
     CDS             12263..12799
                     /locus_tag="PKN22_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNS41293.1"
                     /translation="MKVKNKLYWLKLVAKLAIISALIGFIVVGFKKCTSSEEKEWTLE
                     NHPLKIESIKKIAQLSLINFKDEVVVDSIVYYKNVNEQVVGNVSKIIDPSSWGYALRG
                     SAIEKRLTLIVNGELNYGFDLKSDEFKVNVINDTAHVVVHAPKILSCVVIPSGTSVFL
                     EHGDWKDTARKNFKKRRF"
     gene            12915..>13105
                     /locus_tag="PKN22_00930"
     CDS             12915..>13105
                     /locus_tag="PKN22_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41294.1"
                     /translation="MKYLGIFILLISIWGCSKEIENNKAIDGKWIPVDFYLNDYDGIK
                     HTPTCTGTLIFNNDGKKSKT"
BASE COUNT         4443 a         2047 c         2616 g         3999 t
ORIGIN      
        1 ttaattttta aagataactt ttcaacagca gccatcattt ttgcgttggc tgttgctttg
       61 ttgtttgtag gaaaataccc tttctcacgc atttttgcaa tgggaggaat tatagttgga
      121 ctaggtttgt tggctgttgg ggtgcaaaag gcattgccag agttgaatat tcttccacgt
      181 ttagaaacat gggaaaatcg tatttttaac cgtattgctg cagaaaatga tgtggtagaa
      241 aatgcacaag ttgttaatgc gcaattagct atttacaatg gaggtgtttt tgggcaagga
      301 gtaggtgatg gtaagttaaa agaatattta cctgaagcgt atgcagattt ttattattca
      361 tcatttgtag aggaatttgg atttattgct gccatttttt tggtgtttat ctaccttgtt
      421 ttgctctttc gtatttttag aattgcttta aaatcagata atttatttga aacctatttg
      481 tgtgtgggat tgggaatgtt attcttaacg caagcctctg taaatatgtt ggtttgtaca
      541 ggagtttttc cagttactgg gcaaaatatg cctttactag caatgggagg ttctgccatg
      601 atagtcactt gcacatcatt gggaatgatt caaagtattg ccgttaagca atccaaaaaa
      661 agtaaagaag ataatagtga taataacgac gaagcgtaaa tgaaaaaggt aataatttcg
      721 ggtggtggta caggtggaca tatttttcct gccgttgcaa ttgcagatga attgaaacgt
      781 caatttcctt ccatagaact actttttatt ggagccgaag ggaaaatgga aatggaaaaa
      841 gtgccacaag ctggatataa aattatcggg ttgcctattt ctggtttgca acgaaaaatt
      901 acacttcaaa acttggcttt accgtttaaa atcatcaaaa gtttattgct ggcaagaaaa
      961 gttattaaaa atttcaatcc tgaaattgtt attggtgttg ggggctatgc ttcgggtcca
     1021 actctaaaaa tggccgcctt gttgggtatt ccaaccttgg tgcaagaaca aaattctttt
     1081 gctggtaaaa caaacatgtt gctggcaaaa aaagcacata aattgtgtgt tgcttatgag
     1141 ggaatggaaa agttttttcc aaaaaataaa atagtattaa ccggaaatcc tgttcgtttt
     1201 gaagtaaccc aaattgaagg aaaaagagaa gcagccattg cacattatca attggatgca
     1261 tcaaaacctg ttttgttggt aattggtggt tcattggggg caaaaacatt gaatgagtcg
     1321 attattgctg ggatagaaca atttcaacaa cacggtattc agttggtgtg gcaatgtggt
     1381 aaattgtatt ttgatgattt aaccaatcgt ttaaaaggca aagatataac ggggattcat
     1441 cttacgcagt ttatagcaaa aatggatttg gcctacgctg ctgccgatgt gattatttcg
     1501 cgtgctggtg ccatttctgt ttcagaattg tgcttggtcc ataaacctgt aattttagtg
     1561 ccatccccaa atgtagcaga agaccatcaa acacacaatg caatggcctt agtgaataaa
     1621 aacgcagcca ttttagtgaa agacagcgag gcaagaaatc aattagtaga tgttgcaata
     1681 gatttattaa ataataaaga aaaacaacaa accttagcac aaaatatagc gcaattgggc
     1741 aaaccgaatg ctacaaagga tatcgttgca gaaatagtca aaattgtaaa ataaccgatg
     1801 gaaattagaa aattaaatat cgacttaaag caatttgaaa acatctattt tctaggaata
     1861 ggcggtatcg gcatgtcggc cttggcacgt tattttaaag cgcttggaaa aaatgtagct
     1921 ggttacgata aaacaccttc tcaattaaca gaaagcttgg tgcaggaagg aattgaggtg
     1981 cattttgaag atcgaaattc aagcatcata tcgccatatt gcgataaggc gaaaacgttg
     2041 gttatttata cccctgctat cccaaaaaat catggagaat tgatgcattt ccaacaccaa
     2101 gaatttgtat tggttaaacg ctcagaagtg ttgggtttaa ttacacaaaa ttctttaggt
     2161 ttgggtgttg ctggtacgca tggaaaaaca accacaagta ccatgttggc gcacatcatc
     2221 aaccaaactg aggaaggatg cacagcattt ttaggaggca tttcatccaa ttataattcc
     2281 aatttattgc taaaaccaac gtcccgatat acggtaattg aatccgatga gtttgaccgt
     2341 tcgtttttgc gtttgtcgcc attttcaagc atcataacgt ctgcagatcc cgatcatttg
     2401 gatatttatg gcgatgcagc tcattttaga gaaggttttg aaatgtatgc caatttgcat
     2461 ccagaaaatg gaatagtagt gcaaaaattc gggttgaatt tacccaccaa agcaaaggta
     2521 tatacctatg gtattgaaca aaaagatgcc gattttaatg gtgttaattt gcgttatgtg
     2581 gatgaacaat tttggatgga tgttgttact ccttttggat tttttgaagc cattgaattg
     2641 ggagtacccg gtagtcacaa tgccgaaaat gcagttgctt gcatagcgtt atgtttagaa
     2701 ttgggtgttg aaatggaaac cattcgcaag ggattaaaaa gctttttagg agttaaacgt
     2761 cgctttgaat accatataaa atcgcaaaaa ttaatttata tcgacgatta tgcgcaccac
     2821 ccaacggaat taaaagcgtt gattgattcc attaagttga tctatcctaa tcgaaaaatt
     2881 acaggtgttt ttcagccaca tttgtttacc cgcacacgcg atttttttga tggatttgca
     2941 gagcaattat cgcgttttga cgaagctatt ttaatgccga tttaccctgc tcgcgaagaa
     3001 ccaattgctg gagtaaccag tgatgcattg ctcgataaag ttcatgcgcc tgcaaaacaa
     3061 ttgttagcct ctcaagcagt gatagattac ctatctacaa ttgaagaagg tgtgatttta
     3121 actgttggag ctggtgatat tgatagaatt gttgaaccat taacgaaagt tttaagtgaa
     3181 taaatggatt aaaataacgt tgtggtccat cgttgcaatt gcagtggttg tgggtgtggt
     3241 ttttgctcaa aaagcaatta ataacatgac tttgctagcg cctactatcg aaattagcgt
     3301 tgatggagaa aatgctattc tcaacaaaga agaggtaatg acaaggttgc aacggcaaaa
     3361 tttgatttat gtcaaccaac gtgtaaaaga cctaaatacg gagcgcattg agcggtttat
     3421 ttccaacatg gaagaagtga aaaatgtgaa aatttattca tcttttggtg gaaaatggac
     3481 tatttctatc gtgttaagaa agccattgtt tcatgtttat aatccatcgg gtgaaaatta
     3541 ttatgttgat actgagggat ataaaattag tagtacaatt gaccatgcag cacgtgtttt
     3601 aattattaca gcagataaac cattgaaatt caatcagaaa acagtgtttg aaattattaa
     3661 caacgatagt ttgaaaacta ttcaaaaaat tgataatgtg tatagaattt caaattatgt
     3721 ttgtaatgat ccactaatga ggtctttaat cgggcaaatt catttgaaaa ataacggtga
     3781 ttttgttttg ataccagtag ttggtgatca aactattgtt tttggtatgg caaatactga
     3841 aaaagaaata gagaataaat ttgaaaaatt gaagatattt tacaaagaag cgataccgta
     3901 tgaaggatgg agcacataca gtgagatttc actgaaatac gataagcaga ttgtttgtaa
     3961 gaaaaaagaa taacggaaag tatgtcaaaa gttgttgtag gattggatat tggaaccaca
     4021 aaaatagctt gttttgtggg gcaacgtaat gaatatggta agattgaaat tttagcaatc
     4081 ggccaaaagg aatctcttgg agtaaatcga ggggtggttg aaaacattga aaaaacgatc
     4141 caatctatta aaggagctat cgaagatgct aaagaatata ttgaacagca aaaagatggc
     4201 gttgaaatta cattaagaag tgtttatgtt ggtgttgctg gacagcacat caaaagtatg
     4261 cagcacagag gtatgttagt gcgtaccaat gttgaaaatg aaatttcgca aaaagattta
     4321 gacatattgg aagatgatat gtacagaatg ttaatgcctc ctggagaaca aatcatcaaa
     4381 gttattccaa aacaatacga tattgatggc caccagggaa ttaaagaccc aattggccat
     4441 gctggtgtta aaattgaggc taatttccac atgattactg gaaatgttgg ggcttgtacg
     4501 aatatagtgc gttgtgtaac gcgtgctggg tatcatgttc aagatatggt gttggagcca
     4561 atggcatctg ccgaagctgt gctttctgaa gaagaaaaag aagcaggtgt agtattggtt
     4621 gatataggtg gtggaacaac agatattgcc attttccatg acggtataat tcaacataca
     4681 gcaatcatcc catttggtgg aaatgttatt acacaagata ttaaagaggg atgtcaaatt
     4741 atgcaaaaac aagcagaacg cttgaaagtg aagtttggct ctgcaatcgt caatgaaaat
     4801 caatccaatg aaattgtttg cattccaggc ttaagaggac gtgagcctaa agaaataaca
     4861 gtaagcagct tagcaggaat catcaatgca cgtatgacag aaatcattga gttgattgat
     4921 tacgaaataa ttaatacagg attaaagaaa aaattaatcg gtggaattgt tgtaacaggt
     4981 ggaggatcac aattaaaaca cttgaaacaa ttgtttgaat tccatacagg aatggataca
     5041 cgtattggat atccaactga acatttagca aataataatg aaattgaaaa tatagaaagc
     5101 ccaatttatt caactggtat tggcttggtg ttgacaggat ttaaagaact agataaacgc
     5161 gaaggatatg caataaatgc caatgctgtt aatgctgaaa ttaaaaacga taaagaagag
     5221 aaaaaggtag aagaaccaaa acgcatcaaa gagcgcaaag gattctttga gtcattaatc
     5281 tctaaaagta aagatatagg cagagatttc tttatggacg aagaataagc tgattataat
     5341 aaattataca acaacgaaaa actaaataga atggaatttg aattaccgaa agacaacgct
     5401 tcaatcatca aagtaattgg tgtaggaggt ggtggtagca acgctgttaa tcacatgtat
     5461 aaccaaggaa ttataggtgt agatttcatc gtgtgtaata ccgataaaca agcattggac
     5521 atttcacctg tgccttacaa aattcagtta ggaccatctt taactgaagg aagaggagct
     5581 ggttctattc cagaaattgg taaaaatgct gccgtagaaa acattgaaga aattcaaaac
     5641 ttgctttcaa acaacactaa aatggtgttt attactgcag gaatgggtgg tggtactggt
     5701 actggtgctg ctcctgtaat tgctagtgtt gccaaagaca tgggtatttt aacagttggt
     5761 attgttactg ttccatttgg ttttgaaggt aaaaaacgcc gtcaacaagc ggaagaaggt
     5821 ttggatgaat tgcgcaaaaa cgtggatgct ttgttgatta tcaataacga acgtttgcgt
     5881 gaattaactg gtaatttatc attgagcaat gcgttcgctc aagcagatga cgtgttggca
     5941 gtggctgcta aaggtattgc agaagtaatt tcggttactg gtattatcaa tactgactta
     6001 aatgacgtaa atacagttat gcgtaactct ggtgttgcta tcatgggaag cgctgcggct
     6061 gaaggtgaaa atagatcaat tgttgctgta caaaatgcgt tggcttctcc attgttaaac
     6121 gataacgata tcagtggagc taaatatgtg ttgttgaaca ttacatacgg cgatgctgaa
     6181 attactatgg atgaaatggc agaagttacg gattacatcc aagaagctgc tggttcaact
     6241 gctgaaatta tcttcggaca tggctacgat gcgtctttag gaaataaatt gagcgttact
     6301 gttattgcaa caggtttcgc atcttctcca attacaggtt ttgaaaaagc accagagcaa
     6361 atcaaaaaag tgttggacga agaaccaaaa aatgaaatta aaatgcccat gacttctcca
     6421 actgaatctg ctgtgtggag tggggccaaa gaagtaactg ctgaacaaga agaaccgttc
     6481 atcaagcaag ttgttgaaac gaaagtggaa gataaaccac aatttgtttc aatgccaaca
     6541 gaaccagttg ctgaagtgaa aaaagttaat ttagatgatg aagttacaga acaacaaggt
     6601 gcaattaact ttgactggga agtaagttca ccagcgccaa ttgctgaaac tccatctatt
     6661 cctgttgcag aaaatacaac aagcgaattt gttcgttata cattagatga agaggatgaa
     6721 acgatgaata caacggataa tgttgaatct acagaaaaaa gacacgttct ttcacctgag
     6781 gagcaattga gaaaacaaca agaaagaatg agccgtattc acgaatacac tactcgcttg
     6841 aaaaaagcag atggtgtgat tgaattggaa aaagaaccgg ctttcacaag aagaaatatc
     6901 actattgata acagtaataa ctacagctct tcttctgaat cttcacgttt tggagttaat
     6961 tctgatgaaa atggagggta cagattgaat ggtggaaatt cttttttaca cgataacgta
     7021 gattgataca aatgagtttt acagataaaa taaacgcaga tattaaaacg gcaatgttgg
     7081 ctaaggacaa agaacgctta gccgcattgc gcgatattaa atcaaaatta atgttagaag
     7141 caacttctgg agctggtgaa gtaacagaag aagctgctgc aaaaattgtt ttaaaattac
     7201 acaaacaacg catagatacc tataacttgt acatcgaaca aggtcgccaa gatttagccg
     7261 atgatgaact atttcaagca aaagttatcg aagaatattt gccaaaacaa ctttctgaag
     7321 atgaaatcag aaacgaagta aaagcagcta tccaagcagt tggtgcatct ggaccagcag
     7381 atatgggtaa agtaatggga gttcttacaa aacaattggc cggaaaagca gatggtaaaa
     7441 tcatctcggc aatggtgaaa gaagaattgg ccaaataagc attctacaaa acaacataaa
     7501 tttttcgttg taaaagtcgc tctgttacag gtagagcgac ttttttatta tcgctcaatt
     7561 ttgcttttat gcaatcgatg taaatatact tgctcgcttt gtccgggtgt tggatataaa
     7621 ttcccctcac accccaacgc ttttagatcc atcaaggtgg tgtaaccaca ataactataa
     7681 atggtttttg cttgataaaa caatacatcg ctttctttcc acgaccttgg ttggatggct
     7741 cttttatctt tgcttggcaa cgaggttatg atgtaatcca aatgctttat tgcttccagt
     7801 tgttgtaaaa gctgaaaatt atagggaaaa ggaccgttac aaacccctat gactaatttt
     7861 ttaggagtat ctattgtcgt tgtgtcaaat cgactgaaat gaccaatgta agaactgtta
     7921 gcccaacgat tgtttgcact taatttacct gccaatgatt gtgtagtatc gtctaaaatc
     7981 caaatccaat taaaagcttg catccatcgg ttgtgaatca tttgcgctgg aaattgccac
     8041 cattttaacg ccaaatttac ttggtgggtg ataaaaacag aaggaatgtg tttggaataa
     8101 aacccatatc gatgatcaga aaggacaaca tcaatctggt attctttcac tatattttct
     8161 actaaaacat gttcgttttg taggtgttga agcaatgctt tacgactctt ccataaatca
     8221 acggcaaaat tcccctttcc cgaaaaatga aatgggtagt ttgccaattc cacaaattcc
     8281 aagtcatcca agtattcggc aagcaccaaa aaatcttctt tttcgcaggc tataactaaa
     8341 cgattacctt gttgcaccaa ctgtttgcat ataccaatgg aacgcgataa atgtcccttt
     8401 ccccaattaa gaaaagaata gagaatgcgc gtgttagtta tttcatggag tggtagcaaa
     8461 tttattcttt aatgaatggt tttggtcatg tgctcatcca cacaaataat gaagtgtgct
     8521 ttgtctatat gttctttgtc catttttttg aaatcacttt gttcaaccac tgtaatggtt
     8581 gtgtagtgca aatttggacg caaacgtttt agataccaaa taattccttg gtcaaaatcg
     8641 gtatgaaacg aaccattgaa atgaatgaat ttattggttg gatagttttt ccaatattta
     8701 tcaataaaat gcgccattgt agcgtctttg cttgcttgcg aataaatgaa atttaaacct
     8761 ttgttgtgat cgggcatcat tcccaacatt tttttgtatt gcgaaagtga agtatccacc
     8821 acataatttt tatcacacat gtggttgtaa tctattggtt ttagtgaatc caaagcaaca
     8881 cgcccttttt tgtaaactaa acttgcaatc caacgtggaa tgttagatgc aacacaaatt
     8941 aaattgttgt ccttagcaaa atttacaatc ggtttatagt cagttttata gttgttccac
     9001 aaacgcgtgc tatcttcaaa ttgtttggca gtaatttttc cactttgata atcggaaagc
     9061 aattgttgtt gatcttgttc aaacatttca aagcccaatg taagctggtt gttggttaaa
     9121 cgagccatat ccatcgttag ctccaattct aaccaatggg caattgtgtt gtcatgaaat
     9181 tcaccaaaaa aagtaatgtc agatgctatt gctgcttgaa gcatcttttt gtaacttact
     9241 ttttttcctt ttgcattgta aataacataa gcaggtttgt tttgagaaaa tgttaaaaag
     9301 ctaaagccta ctaaaaggga aacgaggata aatttcatgt ttttttcttt caaaaataca
     9361 catatttacg aaagtgttgg caattacagc accagaatat ttaaaaatga aagaggttgt
     9421 ataagcaaga atgcgatggt ttttgtgtta gatgacttaa aaatcgaaaa ttaattaacc
     9481 aaaaaaccgc atatacccaa tagtgggtat atccactcca tttgataatt gtacatttga
     9541 acaatactta taaaaagtac aaatcttagc aactagaaat tacacctatc cacaatttat
     9601 ataaataaag gctgttctat tgaagaatag cctttgttat atccatcctt tatcttgcgc
     9661 aaataaaacc agctctattg cactttttgt tcccgttttt atatgtatat ttttgcggtg
     9721 agtatttact gtttctgggc taatttgaag ttgcttggca atttctttgg aggttaaatt
     9781 ggtggcaatt aattgaacaa tttccatttc tcgaggagtg agaacagcgg ctaattctat
     9841 gaattctttt tggggactaa cattgataaa cgattgttct ccatctaacc caataaatga
     9901 gagttccatt ttggtatccg attttctttt taaatgtgaa atatcggtat gaatggcaaa
     9961 gtttctcaaa atagcaccct cttcatcggt ttgtatagta gtagattgtt gcaaaatatg
    10021 catgtattca ccattgcttt ttcgaatcct ataattgtaa cgcgatttgt atttcatcac
    10081 tttatctggt ggcaacttca ttttaaaatc aattaccgtg ttttcaaaat caatgaaata
    10141 gggtaaatca tcagggtgaa tgatggataa taatttttca actgtaaatt cagatggttt
    10201 gtatcctaaa atagtttcca tttctgctga aacaaattct attttattca tcggaggtgc
    10261 aaaaataaag taatagaaat caccaacttg cattaattcc attaacttct tttgaatgga
    10321 aatttcacgc tcataatcac ctaaagtttt ataatgccct acttttttcc aattatctaa
    10381 aatgtgtttt tttaaagaat tattcatttt aacatcttgc aagtcaactt ctacgaattt
    10441 aggaaaatat ttctactttt tatcagagaa ttctattttc attcattgaa tttcattttt
    10501 tattaatttt acgcatcatg agtgaaataa aaacacatat tgttcaacgt tctcacgaat
    10561 tgtttgaaaa agtgaaggct tatcgcgagc accttcatca atatccggaa ttgtcatacc
    10621 aagagcatca aaccatgaag tttgtcgctg ataaattaac agaattgggt gttgaacatc
    10681 aaacaggtgt aggtggaaca ggtgtcgtag ggattattaa agcctctcac cacaccaacg
    10741 atatggaagt tgttgccttg cgtgctgata tggacgcatt gcccattcat gaagaaaata
    10801 atgttcccta taaatcaaaa gtggatggcg tgatgcatgc ttgcggacac gatgcacaca
    10861 cagctatttt gttgggaaca gcagaaatac tgcaagaaat taaagagcaa ttgcctcaac
    10921 ctgtcaaact gattttccaa ccgggcgaag aaaaaaatcc aggtggcgca tcgttgatga
    10981 ttaaggatgg agtgttgcaa aatcctactg ttaaaactat ttatggattg catgtttttc
    11041 cagatttacc ggctggaatg acagggttta aaggcggact ctacatggct agttgcgatg
    11101 aaatttatat taccattcac ggtaaaggtg gtcacggagc aactccacac caatgcatcg
    11161 acccaataac gattggtgca gaattgattt taaatttgca aaccatcatc agtagaagac
    11221 gagatccaaa aattccgggt gtattaacct ttggatattt tgaagcattg ggggcaacca
    11281 atatcattcc agccaaagcg gttttgaaag gaacttttcg taccatgaat gaagaatggc
    11341 gtgcagaagc tcttgaatta ttgcaacaac aagccaaagc aatagtggaa ggaaatggcg
    11401 caacgctgga gttggaaatt agcaagggct atccctattt ggaaaacgac gaaacgctaa
    11461 ctgctcaact gaaacaaaaa tctattgatt tattgggtag tgacaatgtg gttgatttac
    11521 ctatccgcat gggaagtgaa gatttttcgt attatgcaca agaaattcca gcttgttttt
    11581 ttagaatagg tgtgagggat gaaaaaaatg gaattatctt tggtgcacac catgcacgtt
    11641 ttgatatcga cccacaagca ttgaaaattg gtatgcaaat gatgagttta gctgtttttt
    11701 aatctatgaa aatattcagt ttttgttttg tatttctttt tgttgtttcc tgtgcaaata
    11761 acgatgcgca agaagtagca aatacgaata ttattcaaat tcaagacatt ggttccttag
    11821 caactacaga atacaccttt agcaaaattg tgcaactcaa cgatgataaa gattggtaca
    11881 aaataggtaa tcgcaaaata ttgttcagtg ttaaagcaaa agtaaaagca ggtatagatt
    11941 ttaataaagt acaaccagaa gatgtaacca ttgatggtaa aaaatccatc cacattaaat
    12001 tgccagaagc cgaaatttta gcctttgaaa ttaatccgga tgatgtgcaa acagaaatgg
    12061 aagatataag cggtttgcgt tttgaattta gccaacagga aaaattgaag attttaaaac
    12121 tgggtgaaaa aagcatcaaa gaagacatgg gaaaaagtgc tattcttcaa gttgctaaaa
    12181 aaaatacaca cgccttttta gttgattttt ataccaacct tggttatgaa tcggttacta
    12241 ttgaaactaa atcagccaaa ttatgaaagt aaaaaacaaa ctttattggt tgaaattagt
    12301 ggctaaactt gcaattatta gcgctttaat tggatttatt gttgttgggt ttaaaaaatg
    12361 tacatccagc gaagaaaaag aatggaccct agaaaatcat cctcttaaaa ttgaaagtat
    12421 taaaaaaatt gcacaactaa gtttaatcaa ctttaaagat gaagtggttg tagattctat
    12481 tgtttattac aaaaacgtta atgaacaagt agttggaaat gtgagtaaaa taatcgatcc
    12541 aagttcttgg ggatatgccc taagaggttc tgcaatagaa aaacgcctca ccttaattgt
    12601 aaatggcgaa ttaaattatg gctttgattt aaaatccgat gaatttaaag tgaatgtaat
    12661 aaacgacacc gcgcatgttg ttgttcatgc ccccaaaatt ttaagttgtg tggttattcc
    12721 ctcaggaaca tctgtttttt tagaacatgg tgattggaag gacacggcac gtaaaaactt
    12781 caaaaaaagg cgattttaaa aattgaaaag catgctcaag caatgcaatt tgatgaaaga
    12841 gcaaaaaaac agtttgaaca attgcttcaa aagttaatca ccaacaaaca tcatttaatt
    12901 gtaacatata aggaatgaaa tatttaggaa ttttcatttt actgatatca atatggggtt
    12961 gctctaaaga aattgagaac aataaagcca ttgatggcaa atggattcct gtagattttt
    13021 acctcaatga ttacgatggc attaaacaca cgcctacctg tacgggaaca ttgattttta
    13081 acaacgatgg caaaaaatca aaaac
//
LOCUS       DAOQVQ010000013        13038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5376_length_13038_cov_3.872449, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000013 DAOQVQ010000000
VERSION     DAOQVQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5376_length_13038_cov_3.872449"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKN22_00935"
     CDS             <1..832
                     /locus_tag="PKN22_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS41295.1"
                     /translation="ISATIIGMMNALDVASTIVGVQEIKYVYNPLVNENFKQHKVIET
                     GYETQLAVEAIIAVQPAVILHNGLNSAFPHQQQFENLGIHCVPIFDWREETPLGKAEW
                     IKVYGFLYGKMEAAVQLFTTIESNYQKLVQQARTLKPSELLMSGNIIGSEWYSPAGGS
                     FYAQLFRDANITYAFSDSKGTGSIANTQENYLVKNRHAKIWINPGARSLKELLAINSK
                     ANLYDAFKEKNVYCYSKNDTYYWEMMSIMPDALLQDLIWITHPELRNGQQLHFYSLLE
                     "
     gene            873..1268
                     /locus_tag="PKN22_00940"
     CDS             873..1268
                     /locus_tag="PKN22_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41296.1"
                     /translation="MKKSEIPQDESALKGWTREVFYAKNEEGKYEAGLSTGWSVKKDA
                     LDGAWEEVHKNVEIAHQLVKDGKMSPIYYFMELNLMDVSVLAQYMGFWKWRVKRHFKP
                     EVFRKLKPTVLQRYATVFEIDVEQLKNFK"
     gene            1270..2277
                     /locus_tag="PKN22_00945"
     CDS             1270..2277
                     /locus_tag="PKN22_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNS41297.1"
                     /translation="MQEFKHYQAEHCENGVTVGLLKQVGLEQITEPLAFGIGSGLFYI
                     EIPFLKLQSGPAISFRTFPGNIFKRTCKAFDVQLYSKKYRDEIKGMNELDALLAQGKS
                     VGCQVGVFHLSYYPKEYRFHFNAHNLIAFGKEGNDYLVSDPVMEDTTRLSQQAMRKVR
                     FAKGVLAPKGHIYYPTSIGIVTQDKLIKAIKTCIKRNCRDMLRIPASFIGTSGINYTA
                     KNIRKWKDKLGDRKAALYLGQIVRMQEEIGTGGGGFRYLYAAFLEEAAAITRNDELLL
                     VSEKITKAGDMWRDSAVQMAGIYKGRLTTQADYNHSADILNAISALEKEAFLELDKIK
                     L"
     gene            2481..3062
                     /locus_tag="PKN22_00950"
     CDS             2481..3062
                     /locus_tag="PKN22_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41298.1"
                     /translation="MMESLVAYERPNDKISELLKSGFLIALRRGLYVPGPEADLSIPD
                     MFVIANHLRGPSYVSLESALSYWGMIPERVVETSSITLKTTKTYNTPIGRFSYRHLAF
                     PYYSFGLQRIELTPRQAAIIATKEKAICDKIILTPGVNLRSQVQTLDFLVDDLRIDED
                     TLATLDVQAIQSWVVDAPKSSSLQMLVKTLQAL"
     gene            3059..3913
                     /locus_tag="PKN22_00955"
     CDS             3059..3913
                     /locus_tag="PKN22_00955"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNS41299.1"
                     /translation="MIKDWIETYRPKNEEEVLSALREIMQEITLAALSRTDFFKKAAF
                     YGGTALRIFYQLDRYSEDLDFSLEEDNPDFSLEPYFEVIQEEFNSFGLTVSLREKSKT
                     IKTPIESAFLKAETIWKELVLEDIVQQVNIRSNRTIKIKIEVDRKPPKGFKTEEKLLL
                     RPYSFYVKCFDLPCLFAGKMHALLYRKWKNRVKGRDWYDMEWYIRKGVPLNLTHFLQR
                     AKDTGDWTDDQITEEQFLAILNDKIDQSSIPHIKDDVLRFVNDASKLEIWSQAYFKDL
                     IGQLKFTH"
     gene            complement(3910..4392)
                     /gene="rnhA"
                     /locus_tag="PKN22_00960"
     CDS             complement(3910..4392)
                     /gene="rnhA"
                     /locus_tag="PKN22_00960"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNS41300.1"
                     /translation="MTTTIEIFTDGAAKGNPGNGGYGALLRFNGKIKELSQGYRKTTN
                     NRMELLAVIVALESLKTNQYPVVVYSDSKYVVDAITQNWLYGWVKTNFKGKKNKDLWL
                     RYLAVAKNFKIEFKWVKGHNGHPENERCDQLAVAAAESNNLLVDEEYEKLESEGGLFD
                     "
     gene            complement(4403..>7267)
                     /gene="ileS"
                     /locus_tag="PKN22_00965"
     CDS             complement(4403..>7267)
                     /gene="ileS"
                     /locus_tag="PKN22_00965"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNS41301.1"
                     /translation="QPYSPKAGTGLSSHELNQPGCYRDVTDTTVVAQFKVKEGQQLPF
                     NYTDAQNTYILAWTTTPWTLPSNTALTVGPKIEYVLVKTFNQYTFREIYVILAKNLVG
                     YQFGGKYFAVENEADLTSFTKEDKKIPYYIIGECLGNDLIGINYEQLLPYALPYENAD
                     KAFKVIPGDFVTTEDGTGVVHTAPTFGADDARVANEAGVPGMLVLDEHEKAVPLVDLQ
                     GKFRPEMGEFAGMFVKNEYYNDGEAPEKSVDVQIAIKLKEENKAFKVEKHVHSYPHCW
                     RTDKPVLYYPLDSWFIRVTDVKERMIALNNTINWKPESTGSGRFGKWLENANDWNLSR
                     SRYWGIPIPIWRTEDGTEEICITSVEQLKQACEKAVEAGFMSANPLASFEVGNMSDAN
                     YSTVDLHKNYVDAIVLVSESGKPMKRESDLIDVWFDSGAMPYAQWHYPFENKELIDNH
                     LSYPADFIAEGVDQTRGWFYTLHAIATMVFDSVAYKNVVSNGLVLDKNGQKMSKRLGN
                     AVDPFDTLSKYGPDATRWYMITNAQPWDNLKFDVDGISEVQRKFFGTLYNTYSFFALY
                     ANIDGFDYTEEEIELAKRPEIDRWILSKLNTLIAQVDEAYSTYEPTKAGRLIQDFVCD
                     QLSNWYVRLCRRRFWKSDDAQDKISAYQTLYTCLETVALLGSPIAPFFMDKLFSDLNA
                     VTNKHAVVSVHLADFPKVDSTIINNRLEAQMDIAQRISSLVLGIRKKERLKVRQPLQK
                     IIVPVLDKTFASDLAHVSDLILSEVNVKELELLEEGNGMFVKQIKPNFKTIGPKYGKQ
                     MKAIAAMVAQFTQDDIASVEANKGWKGEIEGVAIELDNADFEIVAQDIPGWLVSSEGG
                     ITVALDSHLSDELRQEGIAREVVNRIQNLRKDSGLDVTDKIELTIETTEEIKSAIASN
                     QAYICNEVLATNILFAAVNGDAVSVDIEQEGDAKIVLLKQ"
     assembly_gap    7268..7277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7278..7859)
                     /locus_tag="PKN22_00970"
     CDS             complement(<7278..7859)
                     /locus_tag="PKN22_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNS41302.1"
                     /translation="MSKKYQEYKGLNMPELAKEVMQKWDAENVFQQSITSREGKEPYV
                     FFEGPPSANGLPGIHHVMARSIKDIFCRYQTLKGKQVKRKAGWDTHGLPVELGVEKEL
                     GITKEDIGKKISVDDYNKACKQAVMRYTDIWNNLTKEMGYWVDMENPYITYDSKYMES
                     VWWLLGELYSKNLLYNGYTIQPYSPAAGTGLSSH"
     gene            complement(7973..9286)
                     /gene="ahcY"
                     /locus_tag="PKN22_00975"
     CDS             complement(7973..9286)
                     /gene="ahcY"
                     /locus_tag="PKN22_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNS41303.1"
                     /translation="MSNTTEKLAYKVKDISLADWGRKEIKLAEAEMPGLMSLRAEYGA
                     SKPLAGARIAGCLHMTIQTAVLIETLVELGAEVTWSSCNIFSTQDHAAAAIAAAGIPV
                     FAWKGMNEQEFDWCIEQTLFAFEGGKPLNMILDDGGDLTNMVFDRYPELTKDIKGLSE
                     ETTTGVHRLYERVKNGTLVMPAINVNDSVTKSKFDNKYGCKESLVDSIRRATDVMMAG
                     KVAVVCGYGDVGKGSAASLRGAGARVIVTEIDPICALQAAMDGFEVKKLDSVCHNVDI
                     IVTTTGNKDIVVGRHFEKMKDKTIVCNIGHFDNEIDMAWLNKNYGATKYEVKPQVDIY
                     NVKGNEIIILAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWTNSDKYENLVYMLP
                     KHLDEKVAKLHLAKIGVELETLTEEQAKYIDVPVEGPYKAEHYRY"
     gene            complement(9473..>13038)
                     /locus_tag="PKN22_00980"
     CDS             complement(9473..>13038)
                     /locus_tag="PKN22_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41304.1"
                     /translation="ALALNVENIELIQLRTENSKTFYGVDGQYRVQQTGGVFHYKDQN
                     NQWITLQEHATTNGNEYGIFQSELPLKINTVDGLTSMQLQKNGGNYEFGKKVSLDYID
                     KNGSLVKSVFGTFNNAQTSNNKVIFSNAFSGIDRYQQINYWDLTTDYIIKQKLELPDN
                     VETIQFNDRIRMPEDWSIEFGVGQHTKYGWEGSLLIKDENGNVLSEISMPKFYDSSIK
                     KSKMESGHAINGTYKIKKLCTNTYNIQLVVPAKWLNSPNLVYPLIIDPTATNTYASNQ
                     ALQDKNTTFSASCQATMNVTMIATPYQVTGTNTSYKIWAKGYMGYVADIFGNTDVYAD
                     KAEQRSKVGSLNGWTAVQAGVGTNHTGPWTVANNSMDYNLTNQTIANGCYSSQPTLPF
                     YWQGYQTFLPYDTGGGSGNIYTAGCVTDYQELVTNTWIVTVTYTPTADITLSNFNVGA
                     CSNNLYDLSGTVNITNPPATGNLIATTCDGTQVTVASAPFSLGSYPLTMTGLNANGQA
                     CDVELYFSVGACSQVLTQTYTAPSCTTPTCSFSSMTASTTGCDPGNTYTVSGTLTFAN
                     APTTGQLIVTDCSGNTQTFNAPFTSPQNYSISSLTPNGQSCTITAAFTDDAACTINQS
                     YTTPTIPTVNAGNDVTICSGSSTTLTASGASNYVWDNNAGNTASVTVSPSSTTTYIVT
                     ATSTGCTATDQVTVTVAPSLNVTTTGDVTICPGSTATITASGATNYSWNNNAGSSATI
                     NVSPASTTTYIVTGTDANGCSGQAQATVTVANSLTLNATNVSMCEGGNSTITVSGAGT
                     NGTYTWQPATYLSSATGNTVTFTAGATTTYTITGTDANGCTGTTTVTATVNLNPTIDA
                     GVDKIVCTGSNVTLTASGAGSNGTYTWDNNVVNGSAFIPPVGTTTYTVTGTTSNGCSA
                     TDQVTVTVEDTPQISFTATQDQNCSPVTATFTNTSANANNCVWTFSNGQTITNCGPIT
                     QTYNNAGYYGATLQITSPTGCVATLHQDSLVYVEAKPVASFVPSPVIFELSNPQVTFT
                     NHSTGATSYLWDFDDNGATSTQEHPFHKYPETPAAYTVTLYAYSASGCMDSTTYFVKA
                     EEDLLFYIPNTFTPDGDEFNNVFQPVFTSGFDPFDFNMLIFNRWGERVFESKDATIGW
                     DGIFGGTGKMCQDGTYIWKIEFKTLKNDERKTYTGHVNVMR"
BASE COUNT         3909 a         2446 c         2446 g         4227 t
ORIGIN      
        1 catttctgct accattattg gaatgatgaa tgcgctcgat gttgcttcta ccattgttgg
       61 ggtgcaagaa ataaaatacg tgtacaatcc tttggtaaat gaaaatttta agcagcataa
      121 agtcattgaa acgggttatg aaactcaatt ggcagtagaa gccatcatag ctgttcaacc
      181 agcagtaatt ttgcacaacg gactcaattc tgcattcccg catcagcaac aatttgaaaa
      241 tttgggcatt cactgtgttc caatatttga ttggcgcgag gaaactcctt tgggcaaagc
      301 agaatggata aaagtttatg gttttcttta cggaaaaatg gaagctgccg tacaactgtt
      361 tacaaccatt gaaagcaact accaaaaatt ggtacaacaa gcacgcactt taaaaccatc
      421 tgaattgttg atgagtggta acattattgg cagtgagtgg tattctcctg ctggaggtag
      481 tttttatgca caattgttta gagatgccaa tattacctat gctttttcgg attcaaaagg
      541 aactgggagc attgccaata cgcaagaaaa ttacttggta aaaaaccgac atgcaaaaat
      601 ttggatcaac cctggcgctc gttcattaaa agaattattg gcaataaaca gcaaagcaaa
      661 tttatatgat gcatttaaag aaaaaaacgt ttattgttat tctaaaaacg atacgtatta
      721 ttgggaaatg atgtccatca tgcccgatgc cttgttgcaa gatttaattt ggataaccca
      781 tccagaactg cgcaacggtc aacaacttca tttttactcc ttattggaat aacaggataa
      841 ctaaaatcca gcaattttgt atatttgcac ctatgaaaaa gagtgaaatt ccacaagatg
      901 aaagcgcttt aaagggctgg acacgtgaag ttttttatgc taaaaacgag gaaggcaagt
      961 acgaagcagg actaagcact ggttggagcg tgaaaaaaga tgcgttggac ggtgcttggg
     1021 aggaagtgca taaaaatgtg gaaatagctc accaattagt aaaagacggt aaaatgagcc
     1081 caatttatta tttcatggag ttgaatttga tggatgtgag tgtacttgca caatacatgg
     1141 ggttttggaa gtggcgcgtt aaacgtcatt ttaaaccgga agtatttcga aaattaaaac
     1201 ctactgtttt acaacgctat gctactgttt ttgaaataga tgtagaacaa ttaaaaaact
     1261 tcaaataaat tgcaagaatt taagcattat caagcagaac attgtgagaa tggtgtaaca
     1321 gttggtttat taaagcaagt tggtttagaa caaattacag agccgttggc ctttggaatt
     1381 ggttcgggtt tgttttacat tgaaattccg ttcttaaaat tacaaagtgg acctgctatt
     1441 tcatttcgca cctttcctgg aaacattttc aagcgcacgt gcaaggcttt tgacgttcaa
     1501 ttgtattcta aaaaataccg cgacgaaatc aaggggatga atgagttgga tgctttgctt
     1561 gctcaaggca aatcggttgg gtgtcaagtg ggtgtttttc atttatcgta ttatcccaaa
     1621 gaataccgtt ttcatttcaa tgctcataat ttaattgcat ttggcaaaga aggcaatgat
     1681 tatttggttt ctgatcctgt tatggaagac acaacccgat tgtcacaaca agccatgcgt
     1741 aaagttcgat ttgcaaaagg agtgcttgca ccaaaaggac acatttatta tccaacttcg
     1801 attggtattg taacacaaga taaattgata aaagccatta aaacttgtat taaacgcaat
     1861 tgcagagaca tgttgcgcat ccccgcaagt tttattggaa caagtggcat taattatact
     1921 gctaaaaaca tccgaaaatg gaaagataaa ttgggcgatc gtaaagcggc tttgtatttg
     1981 ggtcagattg tgcgcatgca agaagaaatt ggtacaggcg gtggtggttt tcgctacttg
     2041 tatgctgcat ttttggaaga agcagctgct attacacgta acgatgaatt gttgcttgtt
     2101 tcagaaaaaa ttactaaagc gggcgatatg tggcgtgata gcgctgttca aatggcgggt
     2161 atttacaaag gaagattaac tacacaagca gattacaacc attctgccga tattttgaat
     2221 gccatcagtg cattggaaaa agaagcattt ttagaattgg ataaaatcaa actttaaaaa
     2281 agtagataaa tttcaaaata tatataaaaa acacagtgta taatcgggaa taatccgatt
     2341 atgtgtgttt taaataacat gtatatgtaa aaaatataat acaaaatcga aaataatccg
     2401 atttaatgtt ttatatttgt catatgagaa taagaaatta cataaaaaac tatacagatg
     2461 cacccttaac acatcagctt gtgatggagt cgcttgttgc atacgaaaga cccaacgaca
     2521 aaatcagtga gttgcttaaa agtggttttt taattgcgtt aagacgtggt ttgtatgtgc
     2581 cgggcccaga agcagattta tcgattcccg atatgtttgt tatcgccaat catttgcgtg
     2641 gtcctagtta tgtatcatta gaatcggccc tttcttattg ggggatgatt ccagaaagag
     2701 tagttgaaac aagttccatt acattaaaaa ccacaaaaac atataacaca cccattggtc
     2761 ggttttcata taggcatttg gcttttcctt attattcatt tggattgcaa cgcatagagt
     2821 taactcctcg gcaagcggca ataattgcaa caaaagaaaa agcaatttgt gataaaatca
     2881 tcttaacgcc aggagtcaat ttaagaagcc aagttcaaac actggatttt ttagtggatg
     2941 atttgcgcat tgatgaagat acactcgcaa cgttggatgt tcaagccatt caatcgtggg
     3001 tggtggatgc gccaaaaagt tcgagtttac aaatgttggt aaaaacactg caagctttat
     3061 gattaaagat tggatagaaa catatcgtcc taaaaacgaa gaggaagtac tttctgcctt
     3121 gcgtgaaata atgcaagaaa ttacattagc agctctttca cggaccgatt tttttaaaaa
     3181 agcggcattt tatggcggca cagctttgcg aattttttat caattggatc gttattcgga
     3241 agatctggat ttttctcttg aagaagataa tccagatttt tctttggaac cctattttga
     3301 ggtaattcaa gaagaattta attcttttgg gttaaccgtt agcttaagag aaaaatcaaa
     3361 aactataaaa acaccaatag aatccgcttt tttaaaagca gaaaccattt ggaaggaact
     3421 tgttttggaa gatattgtgc aacaagttaa tatacgctcc aatcgcacaa ttaaaatcaa
     3481 aatagaagtt gatagaaaac caccaaaagg atttaaaacc gaagaaaaat tgttgttgcg
     3541 cccttattct ttttatgtga aatgttttga tttaccttgc ttgtttgcag gaaaaatgca
     3601 tgctttattg tatcgcaaat ggaaaaatag ggtaaaagga agggattggt atgatatgga
     3661 atggtatatc agaaagggtg tgcctttgaa tttaacacat tttttgcaac gagcaaaaga
     3721 cacaggtgat tggacggatg accaaattac ggaagaacaa tttttggcaa ttctcaacga
     3781 taaaatagac caaagttcta taccacatat aaaggatgat gtattgcgat ttgtaaacga
     3841 tgcttctaaa ttagaaattt ggagtcaagc gtattttaaa gatttgatag gtcaattaaa
     3901 atttacccat taatcaaaca atccaccttc actttctaat ttttcgtatt cttcatcaac
     3961 caacaaattg ttgctttcgg ctgcagccac cgccaattgg tcgcaacgtt cattttctgg
     4021 gtgaccattg tgtcctttta cccatttgaa ttcaatttta aaatttttgg caacagctaa
     4081 gtagcgcaac cataaatctt tgtttttttt gcctttaaaa ttggttttta cccagccata
     4141 cagccaattt tgagtaatgg catccaccac atattttgaa tcagaataaa caacaacagg
     4201 atattggttg gtttttaaac tttctaaagc aacaatcaca gccaacaatt ccatacggtt
     4261 attggttgtt ttgcgataac cttgtgataa ttcctttatt ttgccattaa aacgcagcaa
     4321 tgcaccataa ccaccatttc ctggattacc ttttgcagca ccatcggtga agatttcaat
     4381 tgttgtggtc atcggcttaa aattactgtt tcaacagcac aattttcgca tccccctctt
     4441 gttcaatgtc aacgcttaca gcatcgccat taacagcagc aaatagaatg ttagttgcca
     4501 acacttcgtt gcagatatac gcttgattgg aagcaattgc cgatttaatt tcttccgttg
     4561 tttcaatggt taattcaatt ttatcagtaa cgtctaaacc actgtctttg cgtaggtttt
     4621 gaatgcggtt aaccacctct ctggcaattc cttcttgacg taattcatct gatagatgac
     4681 tgtctaaagc aacagtaatg ccaccttctg aactaaccaa ccaaccaggg atatcttgcg
     4741 caacgatttc aaaatcagca ttatccaatt caattgcaac accttcaatt tcacccttcc
     4801 aacctttgtt cgcctcaacc gatgcaatgt cgtcttgcgt aaattgagca accatagctg
     4861 caatggcttt catttgttta ccgtattttg gaccaattgt cttgaaatta ggcttaattt
     4921 gtttcacaaa cataccatta ccttcttcca acaattccaa ttctttaacg tttacttcag
     4981 aaagtatcaa atcgcttacg tgtgctaagt cggatgcaaa tgttttgtcc aatacaggaa
     5041 caatgatttt ttgcaacggc tgacgtactt ttaaacgttc tttcttacga attcctaata
     5101 ccaacgatga aatgcgttgt gctatgtcca tttgcgcttc taaacggttg ttgatgattg
     5161 tgctatcaac tttaggaaag tcggctaaat gaacagaaac aacagcgtgt ttattggtta
     5221 cagcgttcaa atcactaaac aatttatcca tgaagaacgg tgcaattgga gatcccaaca
     5281 atgctacagt ttccaaacaa gtgtacaatg tttggtaggc cgaaatttta tcttgagcat
     5341 cgtctgattt ccaaaaacga cgacggcaca aacgcacata ccagttggat aattgatcac
     5401 atacaaaatc ttggattaaa cgacctgctt ttgttggctc gtaagttgaa taagcttcat
     5461 ccacttgagc tatcaaggta tttaatttag ataaaatcca acggtcaatt tctgggcgtt
     5521 ttgccaattc aatttcttcc tctgtataat caaacccgtc aatgttggca tataatgcaa
     5581 agaaagaata ggtgttatac aacgtgccaa aaaacttgcg ttgcacttca gaaatgccgt
     5641 ccacatcaaa ttttaagtta tcccaaggtt gtgcattggt aatcatgtac cagcgtgtag
     5701 catccggacc atattttgaa agtgtatcga atggatcaac ggcattgcct aaacgtttag
     5761 acattttttg accgttttta tccaatacca atccgttaga aaccacgttt ttataggcaa
     5821 cagaatcaaa aaccattgtt gcaattgcgt gcaaggtata aaaccaacct cttgtttggt
     5881 caactccttc tgcaataaaa tccgcagggt agctcaagtg attgtcaatc aattctttat
     5941 tttcaaatgg gtaatgccat tgagcatacg gcattgcacc agagtcaaac caaacgtcaa
     6001 tcaagtcaga ttcacgtttc attggttttc cagactcgct caccaaaaca attgcatcaa
     6061 cgtagttttt gtgtaaatca acggtagaat aattggcatc cgacatgttt ccaacttcaa
     6121 acgaagcaag cggattagcg ctcataaaac cagcttcaac agctttttca caagcttgtt
     6181 ttaattgttc aaccgatgtt atgcaaattt cttcagttcc atcttcagtt ctccaaatag
     6241 gaattggaat tccccagtaa cgtgaacgcg ataagttcca atcgttggca ttttccaacc
     6301 attttccaaa acgtccagag cctgtagatt ctggtttcca attgatggtg ttgttcaatg
     6361 caatcatgcg ttctttcacg tcggtaacac ggataaacca agaatcaagt ggataataca
     6421 atactggttt gtcggttctc caacaatgcg gataactgtg cacgtgtttt tcaaccttaa
     6481 acgctttgtt ttcttctttt aatttgatgg caatttgcac atctactgat ttttcaggtg
     6541 cttcaccatc gttgtaatat tcgtttttca caaacatgcc tgcaaattca cccatttctg
     6601 gtctgaattt tccttgcaaa tcaaccaaag gaacagcttt ttcatgttcg tctaaaacca
     6661 acattcccgg aacacctgct tcgtttgcaa cgcgagcatc atcagcacca aaggtagggg
     6721 cagtgtgtac aactcccgta ccgtcttcgg tggtaacaaa atcaccagga attactttaa
     6781 acgctttgtc tgcattttca tacggcaagg catacggtaa taattgttcg taattgatgc
     6841 ctatcaaatc attacccaaa cattcaccta taatgtagta tggaattttc ttatcttctt
     6901 tggtaaatga agttaaatcg gcttcatttt ctactgcaaa atattttcca ccaaattggt
     6961 aaccaacaag atttttcgca agaattacat aaatttcacg aaaagtatat tgattaaagg
     7021 tttttaccaa cacgtattcg atttttggtc caactgtcaa cgccgtgttg gatggtaaag
     7081 tccatggagt agtggtccaa gctaaaatat aagtattttg agcatccgta taattgaacg
     7141 gtaattgttg accttcttta actttgaact gagcaacaac ggtagtgtca gtaacatcgc
     7201 gatagcaccc cggttgattc aattcgtgag atgacaatcc tgttccagct tttggagaat
     7261 agggttgnnn nnnnnnnatg cgaactgagt ccggtgcctg ctgccggtga atagggttga
     7321 atggtgtatc cgttgtacaa taaatttttg ctgtacaatt cgcccaacaa ccaccaaaca
     7381 gattccatgt attttgagtc gtaggtaatg tatggatttt ccatatctac ccaataaccc
     7441 atttctttgg tgaggttgtt ccaaatgtcg gtatagcgca ttactgcttg cttacaagct
     7501 ttgttgtaat cgtcgacaga tattttttta cctatgtctt ctttggtgat tccaagttct
     7561 ttttcaacac caagttcaac gggcaaaccg tgtgtatccc aacccgcttt gcgttttact
     7621 tgttttcctt taagtgtttg gtaacgacaa aaaatatctt taatggagcg cgccataacg
     7681 tggtgaattc ctggaagtcc gtttgcagaa ggcggacctt caaaaaacac atacggttct
     7741 ttcccttcgc gagaagtaat agattgttga aacacatttt cggcatccca tttttgcatt
     7801 acttcttttg ccaattcagg catgtttaat cctttgtatt cttgatattt tttgctcatt
     7861 gctactattt cttggaatct atcagattgc aaagataatg ataatctgat aattttttgg
     7921 gtataaaaaa aggtgattcc atcgaaccac ctttccactt attttttact gattaatatc
     7981 tgtaatgttc agctttgtat ggtccttcaa ctggaacgtc aatatatttt gcttgttctt
     8041 cagtcaatgt ttccaattca acgccaattt ttgcaaggtg taattttgct actttctcat
     8101 ccaagtgctt aggcaacatg tacactaagt tttcgtattt gtctgagttt gtccacaatt
     8161 caatctgtgc caaagtttgg ttggtaaatg agttagacat tacaaatgat gggtgaccag
     8221 ttgcacatcc taagtttacc aaacgacctt cagccaaaat gatgatttcg ttacctttta
     8281 cgttgtaaat atctacttgt ggttttactt cgtattttgt agcgccatag tttttgttca
     8341 accaagccat gtcgatttcg ttatcgaagt gaccgatatt acaaacgatg gttttatctt
     8401 tcattttttc aaaatggcga ccaacaacga tgtctttatt accagttgta gttacaataa
     8461 tgtcaacatt gtggcaaaca gaatctaatt tcttcacttc aaaaccgtcc attgcagctt
     8521 gtaaagcaca aattgggtcg atttctgtaa caattacacg cgctccagca cctcttaaag
     8581 aagcagcaga acctttacca acgtcaccat aaccacaaac aacagctact ttaccagcca
     8641 tcataacgtc agttgcacgg cggatagagt caaccaaaga ttctttacat ccgtatttgt
     8701 tatcaaattt tgattttgtt acagagtcgt ttacgttgat tgcaggcatt accaatgttc
     8761 catttttaac gcgttcgtat aaacggtgaa caccagttgt agtttcttca gaaagtcctt
     8821 tgatgtcttt tgttaattct gggtaacgat caaaaaccat gtttgttaaa tcgcctccgt
     8881 cgtccaaaat catatttaat ggtttgccac cttcaaaagc aaacaatgtt tgttcaatac
     8941 accagtcaaa ttcttgctca ttcattcctt tccaagcaaa aactggaatt ccagcagcag
     9001 caattgcagc agcagcatga tcttgtgtag agaaaatgtt acaagatgac caagtaactt
     9061 cagctcctag ttcaaccaat gtttcaatca acacagcggt ttgaatagtc atgtgcaaac
     9121 atcctgcaat acgtgctcca gccaatggtt tagaagctcc atattctgca cgaagcgaca
     9181 ttaatcctgg catttctgct tcggctaatt taatttcttt tcttccccag tcggccaaac
     9241 tgatgtcttt tactttgtaa gccaattttt cggttgtatt gctcatatta gtatttaaat
     9301 ttcaattttt tagagtgcaa atttacaaag ttcctttggg ataaaaaaat gttttatcca
     9361 ctttataaat gattattggg tttgtaaatt atcaggaaca aaaaaatcct acttcaattg
     9421 cctgtatttt tgtaccgctt gaagtaggat tgacgtgttt aacttaaagt ttttaacgca
     9481 ttacatttac gtgaccggtg tatgttttac gttcgtcgtt tttcaatgtt ttgaattcaa
     9541 ttttccaaat ataagttcca tcttgacaca ttttccctgt tccgccaaat ataccgtccc
     9601 atccaattgt tgcatcttta gattcaaata cacgctcgcc ccatcggttg aaaatcaaca
     9661 tattgaagtc gaaaggatca aagccagatg taaacaccgg ttggaaaaca ttgttaaact
     9721 catcaccatc tggtgtaaat gtgttgggaa tgtaaaacaa taaatcttcc tctgctttca
     9781 caaaataagt tgttgaatcc atacatccac ttgcagaata agcatacaag gtaacagtat
     9841 atgcagctgg tgtttctggg tatttgtgga atggatgttc ttgggttgaa gttgctccat
     9901 tgtcgtcaaa atcccataga tacgatgtag ctccagtaga atgattggta aatgtaactt
     9961 gcggattact caattcaaaa attacaggac ttggcacaaa agaggcaaca ggttttgctt
    10021 ctacataaac caatgaatct tgatgcaaag ttgctacaca tcctgttgga gaagtaattt
    10081 gcaaagttgc tccatagtag cctgcgttgt tatatgtttg tgtaattggc ccgcaattgg
    10141 ttattgtttg tccattgcta aacgtccata cacaattgtt tgcattggct gaagtattgg
    10201 taaatgttgc tgtaacagga ctacaatttt gatcttgcgt agccgtgaaa ctaatttgtg
    10261 gtgtatcttc tacagtaacc gtaacttggt ctgttgctga acatccattg cttgttgttc
    10321 ccgttacggt ataagtagtt gttccaactg gagggataaa tgcactacca tttactacgt
    10381 tattatccca tgtgtatgtg ccatttgaac ctgcacctga tgccgttaag gttacattgc
    10441 ttcctgtaca aacaatttta tcaacccctg catcaattgt tgggttaagg ttgacagttg
    10501 ctgtaactgt tgttgtacct gtacatccgt tggcatctgt tccagtaata gtgtatgttg
    10561 tagttgcacc agctgtaaag gttacagtat ttcctgttgc gctacttaaa taagttgcag
    10621 gctgccaagt gtaagttcca tttgttccag caccagaaac ggtgattgtg ctattaccgc
    10681 cttcgcacat tgaaacattg gtagcgttta acgttaaact gttggcaact gttactgttg
    10741 cttgtgcttg acctgaacaa ccattggcat ctgtacccgt tacaatatat gttgtagttg
    10801 atgctggaga aacatttatc gttgcagaac tacccgcatt gttattccat gaataattgg
    10861 ttgctccaga tgcagttatt gttgctgttg aacctggaca aatcgtaaca tcacctgttg
    10921 ttgtgacatt taaacttggt gcaacagtta ccgtaacttg atcggttgct gtacatccag
    10981 tgcttgtagc tgtaacaata taggttgtgg tagatgaagg agacactgtt acacttgctg
    11041 tattaccagc attgttatcc catacatagt tgcttgcgcc agaggcggta agtgttgttg
    11101 aacttccact acaaatagtc acatcatttc ccgcattgac agtcggtatt gttggtgtgg
    11161 tataactttg gttaatggta caagcagcat cgtcagtgaa agctgctgtg attgtacacg
    11221 attgtccgtt tggtgtaagt gaactaatag aataattttg tggactggta aacggtgcat
    11281 tgaatgtttg tgtattgccc gagcaatcgg ttacaattaa ttgccctgtt gtaggtgcat
    11341 ttgcaaacgt caaagtacct gaaacagtgt aggtatttcc tggatcacat ccggtggtgg
    11401 atgcagtcat gcttgagaaa ctacatgttg gtgttgtaca tgacggtgca gtgtatgttt
    11461 gtgttaatac ttgagaacaa gcgcccacag aaaaataaag ttcaacatca catgcttgac
    11521 cgttggcgtt taatcctgtc attgttaatg gataagaacc caaactaaat ggagcagagg
    11581 caacagtcac ttgcgtacca tcacatgtag tggcaattaa gttgcctgtt gcaggaggat
    11641 tggtaatgtt tactgttcca gacaaatcgt aaaggttatt ggaacaagct cctacattaa
    11701 agtttgacag tgtaatatca gcagttggtg tatatgtaac ggtaacaatc catgtatttg
    11761 taaccaattc ttggtaatca gttacacaac cagcagtata aatatttcca gaaccaccac
    11821 ctgtatcgta aggtaaaaaa gtttggtatc cttgccaata aaaaggcaaa gtaggttggc
    11881 tgctataaca accattggca attgtttgat tagtgaggtt gtaatccata ctgttgttag
    11941 caactgtcca aggacccgta tggtttgttc caacaccagc ttgaacagcg gtccaaccat
    12001 ttaatgagcc cacctttgaa cgttgttcgg ctttatctgc atatacatcg gtattaccaa
    12061 aaatatctgc aacatagccc atatatcctt ttgcccaaat tttataggaa gtattggtac
    12121 cagtaacttg atatggagtt gcaatcatcg taacattcat tgttgcttgg caacttgcag
    12181 aaaatgttgt gtttttatct tgcaacgctt gattggatgc atacgtattg gttgccgttg
    12241 gatcaatgat taacggataa actaaattgg ggctgttaag ccattttgct ggtactacca
    12301 actgaatgtt gtaggtattt gtgcacagtt ttttgatttt ataagttccg ttgatggcat
    12361 gtccactttc cattttactt ttcttaatgc ttgaatcgta aaatttaggc attgaaattt
    12421 ctgaaagcac attgccattt tcatctttaa tcaataaact tccttcccaa ccatattttg
    12481 tgtgttgacc tactccaaat tcaattgacc aatcttctgg catacgtatg cgatcattga
    12541 attgaatagt ttccacatta tcaggaagtt ctaatttttg tttaatgatg taatcggttg
    12601 ttaaatccca gtaattaatt tgttgatagc ggtcaattcc actaaaagca ttgctaaata
    12661 taactttgtt gttggatgtt tgtgcattat taaatgttcc aaacacactt tttacaagag
    12721 acccattttt atcgatgtaa tcaagagaaa ctttttttcc aaattcatag ttgccaccgt
    12781 ttttttgtaa ttgcatggat gttaaaccat caacagtgtt tatttttaac ggcaattcag
    12841 attgaaaaat tccatattca ttgccattgg ttgttgcatg ttcttgcagg gttatccatt
    12901 ggttgttttg atctttataa tgaaaaacac ctcctgtttg ctgcacgcgg tattgtccat
    12961 caaccccata aaaagtttta gaattttctg ttcttaattg aattaattca atattctcaa
    13021 cattaagtgc taaagcat
//
LOCUS       DAOQVQ010000014        12979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5416_length_12979_cov_3.571882, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000014 DAOQVQ010000000
VERSION     DAOQVQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5416_length_12979_cov_3.571882"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="PKN22_00985"
     CDS             <1..1830
                     /locus_tag="PKN22_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNS41305.1"
                     /translation="DQVAILNRDYRRLNADANNVNANFTGMPADVSIEFKLATIAPNG
                     TCFKGITRTVSSLTVDPGGYNGGAQQLNAIFAGNDVYQGTWPHNKYLNVVVAKDIGGA
                     AGYTMYPNNGGTSLNSIFILSTYVGSIGTGNASTSRALTHEVGHWLNLSHVWGDNNNP
                     GEACGDDQVSDTPITKGFTFCPSEANAKVCNASIVENYENYMDYSYCSKMFTPGQVTR
                     MRAAIVGSTGGRNNIWTTANLNAVGAVANPPLCKADFSADKRVVCVGQTVNFTDMSYN
                     LPVASWSWQFQGGTPATSTDQNPSVVYNTPGVYNVTLTATRGGSNPSETKTGYIVVMQ
                     PGSALPYFEGFEGYTNIAQATTQGKLFIENLGNNAGFDITTTAASSGSKSIKLNNFSQ
                     TGSNVDELISNAIDLSNGSINNVTFSFKYAYRKKAANNIEYLKVYFSGDCGETWSLRK
                     PMSSSSMSTATSSSAWTPTAADWKETTIEFNSIAYNQFLTPNFRYRFVFEGNGGNNIY
                     LDDINITLNNASVESQDALSNIALYPNPNDGEVNLTFGLNTAQKVTVKVLDISGKELK
                     STILNGNAGENQIKLDNSEFSAGMYFVNLSTETTNKTLQFIKK"
     gene            1896..3167
                     /gene="kynU"
                     /locus_tag="PKN22_00990"
     CDS             1896..3167
                     /gene="kynU"
                     /locus_tag="PKN22_00990"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNS41306.1"
                     /translation="MENTLAYAQQCDKNDALATFRNQFLFPTFHDKEVKYFTGNSLGL
                     QPKATREYIQTELDTWAKYGVEGHFMGKNPWFSYHELLTEKTAKIVGALPSEVVVTHS
                     LTTNLHLLMVSFFQPKGKRTKILCEAKSFPSDRYALESQLQFHGLSVDEHLIEVAPRV
                     GEHLIREEDILTAIYDNSDEIALIMIGGVNYYTGQLFDMEKITAAGHSIGAIVGWDLA
                     HAAGNINLKLHEWNVDFAAWCGYKYLNSSPGGVSGLFVHQRHEYKPELPRFAGWWGYD
                     KATRFQMEPGFNPIKGAEGWQLSNAPVLGMAAHLASLDIFDQAGMEAIGKKRDEMTAF
                     LEFVINDVSERNKEKVTFEIITPSSPKERGAQLSILVHGKGKALFDALSEQGVVADWR
                     EPNVIRFAPAPLYNSFEDCYWFGVFLEKAIN"
     gene            3248..5770
                     /locus_tag="PKN22_00995"
     CDS             3248..5770
                     /locus_tag="PKN22_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNS41307.1"
                     /translation="MAEENEIKVKVTKKEEQETKKGAATASKKEAKSSVKSKINVQKI
                     KTVFGVLLALLSVYLFLAFVSYLFNWQEDQDRVVNKGLFEFLFDQDEEPVANWLGKFG
                     AWASHLFMYRWFGISSFGFCLLFFISGFKIALNIRVLPILKTYGITLVFIVWSSIFLG
                     FFANGVNYLGGAFGYQINQWLALTLGSFGSFILILALLYIAMISVLNTDIFKLLALFK
                     RKESDEEADEEPIVASTPTDFLDINAINTIHENQIKEDKISQAVHFDEEEDDADLTDD
                     EIDEGFEVKFAQPEAVTPPPPPPVTPTKVEIEGDDDFSVDVAPEEESIAEDQLDKLRQ
                     EFGEYDPTLDLSSYVLPPIELLNDYGGKGISVNKTELEDNKNRIVETLANYKIDIDKI
                     KATVGPTVTLYEIVPAPGVRISKIKNLEDDIALSLSALGIRIIAPIPGKGTIGIEVPN
                     SNPDMVSMRALIASEKFQNMDAELPVVVGKTITNETFTFDLTKTPHLLVAGATGQGKS
                     VGLNAILVSLLYKKHPAQVKFVLVDPKKVELTLYNKIERHFLAKLPGEGDAIITDTSK
                     VVNTLNSLCIEMDARYELLKDAQCRTIKEYNAKFIQRRLNPENGHRYLPYIVVLIDEF
                     ADLIMTAGKEVEHPIARIAQLARAIGIHLIVATQRPSVNVITGMIKANFPARIAFRVL
                     SKIDSRTILDASGADQLIGRGDMLISMGSDLVRLQCGFVDTPEVEKICDFIGEQRAYP
                     EAMLLPEYTGEGGDGQGGDFDSDELDALFADAARIVVSTQQGSASMLQRKLKLGYNRA
                     GRLVDQLEGMGIIGPFQGSKAREVLYTDLVALEEYLASKGLV"
     gene            5771..6715
                     /locus_tag="PKN22_01000"
     CDS             5771..6715
                     /locus_tag="PKN22_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNS41308.1"
                     /translation="MNVLKLDSFVLAIIASVVLAYFFPFLAHALPLDTISSVGISLIF
                     FFYGLKLSPEKIKAGLANWKLHVVVQLSTFVLYPLLVVAFYPLFKGTSHEIMWFAFLF
                     LAALPSTVSSSVVMTSLAKGNVPAAIFNASISGLIGILVTPLWVGLFLKTTSTQFDMS
                     AIYLKLLLEIVVPVSLGLLLQRFWGAFAQQHLRAITLFDKSVILLIVYKSFVASFEEN
                     VFSTVSWTTFVLVTLVVILSFFLVYGLIGLISKLLHFNREDTIVAQFCGTKKSLVHGT
                     VFSKVLFPSTFPVGIILLPLMLFHAIQLLVVSIIATRK"
     gene            6829..7083
                     /locus_tag="PKN22_01005"
     CDS             6829..7083
                     /locus_tag="PKN22_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HNS41309.1"
                     /translation="MNTKLTLTIDQSIIEKAKKYARKKERSLSDLIENSLKALTNDDS
                     SQTEQELSPTLKSLKGSFKEPKNFDYKKELTDRLSKKYLE"
     gene            7080..7499
                     /locus_tag="PKN22_01010"
     CDS             7080..7499
                     /locus_tag="PKN22_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNS41310.1"
                     /translation="MTKVLIDTDVILDFLFDRKPYSDNATKILALCESGAINGFVTSI
                     MLSNIYYLLRKTASHVKVIESLKMLLQFLEVQTTDKKAVLAALKSDFKDFEDALQYFS
                     AQQDSEISVIITRNVKDYKTSKLAIMPPEIYLKSSAR"
     gene            complement(7529..9469)
                     /locus_tag="PKN22_01015"
     CDS             complement(7529..9469)
                     /locus_tag="PKN22_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS41311.1"
                     /translation="MINFENLGLHLPQGYLFQNIGLQINKNDKVGLVGKNGAGKSTML
                     RILSGMIQPSEGKIHKPKDCTIGFLTQDIVIDTTQSVFEYLNNSNEKLTKLRNELDDI
                     NHQLVSRTDYESDSYLGLLDRLNECNHEFNIIDGYAWEEKIANTLKGLGFEEEEFSRS
                     LNTFSGGWKMRAELAKILVNNPDVILLDEPTNHLDIISIAWLENYLKTFEGAVIVISH
                     DRLFLDNVTKRTLEINNGRVLDFPYPYTKYKQVRAEEIERMNAAKKQQDKEIKQTQEL
                     IDKYRAKANKAAFAQSLIKKLEKTERIEVDNDNVTKMHVTFPLSVQPGKWVLEMIDMG
                     KTFDQKLLFKDINLTVGRGEKIALLGPNGVGKSTLLKRIMGQLDGEGTVNYGHNVNIT
                     YFAQNQAEMLDKTKTVFQTVDDVAEGEIRKNLRTILGTFLFSGDDVEKQVGVLSGGER
                     TRLALCQLLLSPSNFLILDEPTNHLDIQSKEVLKQALKNYEGTFIVVSHDREFLDDLT
                     NRIWDIEDKTLKIHHFGVKEFLERKMNLANAKDSLKKPAKKEEPAVKVETPVNTELDR
                     EQQKELKKQKNALSNKINFAEKEIERLEAKIAEMDVVMADLDYTDEAKAQKILADYAQ
                     LKSQLDEQMQAWEAASEELMGL"
     gene            9664..12024
                     /locus_tag="PKN22_01020"
     CDS             9664..12024
                     /locus_tag="PKN22_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNS41312.1"
                     /translation="MKTLLFIVAVLATFQLSAQSYSRVKIYTDQNGIAQLLNAGVAVD
                     HGIRKEGVFIISELAQRDIQKLNELHFNYEVLIDDVSQFYVEQNKTANQIAKNTTCSA
                     STGVPGQTNIPTNFDVQSTYGGFYKYNQMLQELDDMAALYPNLISTKAQIDTFLTWEN
                     RPIYYVKISNNPTIDNGKPMVLYSAIHHAREPIGMSEVLYYMWYLLENYATNPEVKFL
                     VDNTQMIFVPCINPDGYIHNETTNPNGGGMHRKNKNPNVGSSNPGVDLNRNYGYQWNT
                     TGVSADEDNDTYPGSSAFSEPETHAMRWLVQNMPIRAALNAHSYSNLLLYPLGNATSA
                     FAPHHTYFQALSEYMVQYNGYTAQKSSALYPASGDSDDYMYIMDNGVLEKDTIFAMTP
                     EIGSSFWPASSEIVPLCQGMLFVNLSLAHMVHRLYVVTENDPATIATTTGYFHHTVKK
                     LGRENGSATVSIEPLLNITTVGAPVVYNLATLGSGNDSIAFTLNSSIQAGDTIQYVLT
                     TDDGEWIKRDTIVKLFGGAGDTLIFENGDALTNWTGGWTTSSTIYYSPSKSITDSPGN
                     YANNTTKIINYATAIDLTDATDASASFYARWDIEKEYDYCQFQVSTDNGATWVAQCGN
                     YTGFSTNQNGTVQPQNEPLWDGTKTSWVEEHINLSDYLGQTLQIRFILQSDGGVRKDG
                     FYFDDFKIIAALDTTTTGNAGLNNLQSAIITVSPNPSNDNLTVKNAQANDAILLYDLQ
                     GKLVIATQQQGENPTKVDVSSLENGVYLLKVEGTTSNFIEQVVVAH"
     gene            complement(12050..>12979)
                     /locus_tag="PKN22_01025"
     CDS             complement(12050..>12979)
                     /locus_tag="PKN22_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS41313.1"
                     /translation="ITTLLVITGLLIICFAPVFYSYSERFSGHAEEAEQIRIIGWLAG
                     LLVVIVGLLTAFFKGSKEKINWGAMQYPQLTLGMLAIFIYVGVEVAIGSNLGEYLKIN
                     QNMSASEISPYISMYWGSLMIGRWTGAVAAFDISSRMKNILRFIVPFLAFFVVLGAGY
                     LIGYDLEHLHWYIICVAIQIVAFYLTNDKPALTLGVFGILGALAITIGIFSSGTVAIY
                     AFLSGGLFCSIMWPCIFSLALGGLGKYQTQGSAFLVMMILGGAIIPPLQGKFADIFSI
                     QSSFIVGIVCFAYLAFYAFYVVKLLRKQGLSFE"
BASE COUNT         4076 a         2362 c         2549 g         3992 t
ORIGIN      
        1 gatcaagttg ctattttgaa tagagattac cgtagattga acgctgatgc aaataatgta
       61 aatgcaaatt ttacgggaat gccagcagat gtgagtattg agtttaagtt agctacaatt
      121 gctcctaatg gtacttgttt taaagggatt acaagaacag tttcttcttt aactgttgat
      181 ccaggaggtt acaatggagg tgctcaacaa ttgaatgcta tttttgctgg aaatgatgtt
      241 tatcaaggta catggccaca taataaatat ttgaacgtag ttgtagcaaa agatattgga
      301 ggtgctgcag gttacaccat gtatccaaat aatgggggaa catctttaaa ttctattttt
      361 attttaagta cttatgttgg tagcattgga acagggaatg cttcaacaag tagagcttta
      421 acacacgaag ttggtcactg gttaaattta tcgcatgttt ggggtgataa caacaatcct
      481 ggcgaagctt gtggtgacga ccaagtaagt gacacaccta ttacaaaagg ttttactttt
      541 tgcccgtcag aagcaaatgc taaagtttgt aatgctagta ttgtagagaa ttacgaaaat
      601 tatatggatt attcgtattg ttctaaaatg tttactcctg gacaagtaac aagaatgcgt
      661 gcagcaattg taggttcaac tggaggtaga aataacattt ggacaacagc caatttgaac
      721 gcagtggggg cagttgcaaa cccacctttg tgtaaagcag atttttcggc agataagaga
      781 gtggtttgtg ttggacaaac tgttaatttt acagatatga gctacaattt accagtagct
      841 agctggagtt ggcaatttca aggaggaaca cctgcaacat ctacagatca aaatccttct
      901 gtagtttaca atacacctgg tgtatataac gtaacgttaa ctgcaacaag aggtggaagt
      961 aacccatcag aaactaaaac aggatatatt gttgtaatgc aaccgggttc tgctttgcca
     1021 tattttgaag gttttgaagg atatacaaac attgcacaag caactacgca aggaaaattg
     1081 tttattgaaa atctaggtaa taacgctggc tttgatatca caacaacagc agcaagttca
     1141 ggtagtaagt ctatcaaatt aaacaacttc tcacaaacag gctcaaatgt agatgaattg
     1201 atttcaaatg caattgattt gtcaaatggt tcaatcaaca atgtaacatt ctctttcaaa
     1261 tatgcatacc gtaaaaaagc agcaaataac attgaatact taaaagtgta tttctcagga
     1321 gattgtggag aaacttggtc gttgagaaaa ccaatgagct ccagttcaat gtcaacggca
     1381 acttcttctt cagcttggac accaaccgca gcagattgga aggaaacaac gattgagttt
     1441 aatagtattg cttacaacca attcttaact ccaaatttcc gttaccgttt tgtttttgaa
     1501 ggtaatggtg gaaataatat ttatttggac gatattaaca tcacgcttaa caatgcaagt
     1561 gttgaaagtc aagatgcttt gagtaacatt gctttgtatc cgaatccaaa tgatggagaa
     1621 gttaacttga cgtttggatt aaatacagct caaaaagtta cagttaaagt cttggatatt
     1681 tctggaaaag aattgaaatc tactattttg aatggaaatg ctggagaaaa tcaaattaaa
     1741 ttggataact ctgaattttc tgcaggaatg tattttgtaa acttaagtac agaaacaaca
     1801 aataaaactt tgcagttcat taaaaaataa ataattcaag tatatttaga gtccgtttca
     1861 atagaggcgg acttttttat tttcaaaaaa aaacgatgga aaacacacta gcatacgcac
     1921 aacaatgcga taaaaacgat gcattggcaa cttttagaaa tcaatttttg tttccaactt
     1981 ttcacgataa agaggtgaaa tattttaccg ggaactcttt ggggttacaa ccaaaagcaa
     2041 cacgcgaata cattcaaacc gagttggata cttgggcaaa atatggtgtt gaaggacatt
     2101 ttatgggaaa aaacccatgg ttttcttacc acgaattgtt aaccgaaaaa acagctaaaa
     2161 ttgttggagc tttaccatcc gaagtggttg taacacactc gttgacaact aatttacatt
     2221 tgttaatggt ttcttttttt cagcctaaag gaaaaagaac taaaattttg tgcgaagcca
     2281 aatcgtttcc aagtgatcgt tatgcgttgg aatcgcaatt gcaatttcac ggactttcgg
     2341 tggatgaaca tttaattgaa gtagcccctc gagttggaga acatttaatt cgtgaagaag
     2401 atattttaac tgccatttac gacaacagcg atgaaattgc tctcatcatg ataggtggcg
     2461 ttaactatta cacggggcag ttatttgaca tggaaaaaat cacggctgcg gggcattcca
     2521 tcggagccat tgttggttgg gatttggcac atgctgcggg caacatcaac ttgaaattgc
     2581 acgaatggaa cgtggatttt gcagcatggt gtggttacaa atatttgaat tcatcgccgg
     2641 gcggtgtttc gggtttgttt gtgcaccaac gccatgaata caagccagaa ttgccacgtt
     2701 ttgcagggtg gtggggatat gacaaagcaa cacgttttca gatggaacca ggtttcaacc
     2761 caatcaaagg agctgaaggt tggcaattga gcaatgctcc agttttggga atggcagcac
     2821 acttagcgtc attggatatt tttgatcaag ctggaatgga agccattgga aagaaacgcg
     2881 atgaaatgac ggctttctta gaatttgtaa tcaacgatgt atcggagcgc aataaggaaa
     2941 aagttacgtt tgaaatcatt acgccaagtt caccaaaaga acgtggtgca cagttatcta
     3001 ttttagttca tggcaaagga aaagcgttgt ttgatgccct ttcagaacaa ggtgtagttg
     3061 ccgattggcg cgaacccaat gtgattcggt tcgcgccagc gccattgtat aactcgtttg
     3121 aagattgcta ttggtttggc gtgtttttag aaaaagccat caattaacat tctggtgtgt
     3181 aaattacact aaaatcaatt gtttgtgcgt ttaacttttg agtaatttcg tagaatagag
     3241 gaaataaatg gcagaagaaa acgaaataaa agttaaagtc actaaaaaag aagagcaaga
     3301 aacaaaaaaa ggtgctgcaa ctgcttccaa aaaagaggct aaatcgagcg tgaaaagtaa
     3361 aattaatgtc caaaaaatta aaacagtttt tggcgtttta ctggcgttgc tttctgttta
     3421 tttgtttttg gcgtttgttt cttatttgtt taactggcaa gaagatcaag acagagttgt
     3481 aaataaagga ctttttgagt ttttatttga ccaagatgaa gaacctgtgg ctaactggtt
     3541 agggaagttt ggagcatggg cgtcgcatct ttttatgtac cgttggtttg gaatttcttc
     3601 ttttggcttt tgcttgttgt ttttcatttc aggctttaaa atagcactca atattcgcgt
     3661 tttaccaatt ttaaaaacgt atggaattac ccttgtattc atcgtttggt cgtctatttt
     3721 tcttggtttt tttgccaatg gggttaatta tttaggtggt gcctttggat accaaataaa
     3781 ccaatggtta gcattaacac ttggaagttt tggtagtttt atccttattt tagccttgtt
     3841 atacatcgcc atgatttcgg ttttaaacac cgatattttt aaattattag cactttttaa
     3901 acgcaaggaa agtgatgaag aagcagatga agagccaata gttgcttcaa ctccaaccga
     3961 ttttttggat atcaatgcaa ttaacaccat ccacgaaaat caaatcaagg aagataaaat
     4021 ttcgcaagct gttcattttg atgaagaaga agatgatgct gatttaaccg atgatgaaat
     4081 tgacgaaggt tttgaagtga aatttgcaca accagaagcc gttactccac caccaccgcc
     4141 gccagttaca ccaacaaaag ttgaaataga gggcgacgat gatttttcag tagatgtggc
     4201 accagaagaa gaatccattg ccgaagatca gttggataaa ttgcgtcaag aatttggaga
     4261 atacgatcca acccttgatt tatcgagcta tgtgttgcca ccaattgaat tgttgaacga
     4321 ctatggtgga aaaggaattt cagttaacaa aacagaatta gaagataaca aaaaccgcat
     4381 tgttgaaact cttgccaact ataaaattga tattgataaa atcaaagcaa cagtaggtcc
     4441 aaccgttaca ttgtacgaaa ttgtacctgc tccgggtgtg cgtatttcta aaattaaaaa
     4501 cttagaagac gacattgcct tgagtttatc tgcacttggt attcgtatca ttgcgcctat
     4561 tcctggaaag ggaactattg gtatagaagt tcctaatagc aatcccgata tggtgagcat
     4621 gcgcgcattg attgcatctg aaaaattcca aaacatggat gccgaattgc cggtagttgt
     4681 tggaaaaacc attaccaatg aaacatttac gtttgacctt accaaaactc cacacttgtt
     4741 ggtggctggt gctacggggc aaggtaaatc ggttggattg aacgccattt tagtgtcgtt
     4801 gctttacaaa aaacatccag ctcaagtgaa atttgtgttg gtggatccta aaaaagtaga
     4861 attaacgctt tacaataaaa ttgaacgcca tttcttggca aaacttcctg gcgaaggcga
     4921 tgcaattatt accgatactt ccaaagttgt caatacgctc aattcgttgt gtattgaaat
     4981 ggatgcgcgt tatgaattgc tcaaagatgc acaatgtaga accatcaaag aatacaacgc
     5041 taagtttatc caacgccgtt tgaatccaga aaatggtcac cgttacttac cttacattgt
     5101 ggtgcttatt gacgagtttg ccgacttgat tatgacggca ggcaaggaag tagaacatcc
     5161 aattgctcgt attgctcaat tggctcgtgc gattggtatt cacttaattg tggcaacgca
     5221 acgtccatcg gtgaatgtca tcacgggtat gattaaagcc aacttcccag ctcgaattgc
     5281 atttagagta ttgtctaaaa ttgactcccg caccatttta gatgcttcgg gtgccgacca
     5341 gttgattggt cgtggagata tgttgatttc tatgggttct gacttagtgc gtttgcaatg
     5401 tggatttgtg gatacgccag aagttgaaaa aatatgtgat tttattggtg aacaacgtgc
     5461 ctatccagaa gccatgctct tgccagaata tacaggtgaa ggtggtgatg gacaaggtgg
     5521 cgattttgat tcggatgaat tggatgctct ttttgctgat gctgcacgca ttgttgtttc
     5581 cactcaacaa ggttcggcaa gtatgctgca acgtaagtta aaattaggct acaaccgtgc
     5641 aggacgattg gtggatcaat tggaaggaat gggcatcatt ggtcctttcc aagggagcaa
     5701 agctcgtgaa gtattgtaca ccgacttagt tgcgttagaa gaatatttag caagcaaagg
     5761 tttggtttaa atgaacgtgt taaaattgga tagttttgtg cttgccatca ttgcaagtgt
     5821 agttttagcc tattttttcc cgtttttagc acatgcatta cccttagaca ccattagtag
     5881 cgttggtatt tcgttgattt ttttctttta tggcctaaaa ttatcgcccg aaaaaataaa
     5941 ggcaggtttg gctaattgga aattacacgt ggtggtgcaa ctctccactt ttgtattgta
     6001 tccgcttttg gtggtggcgt tctatccttt gtttaaagga acttcgcacg aaattatgtg
     6061 gtttgcattt ttgtttttag ccgctttgcc ctccaccgta tcctcgtcgg tggtaatgac
     6121 ttcgttggca aaaggaaacg tgccagctgc catttttaac gctagtattt ctggtttaat
     6181 tggtattttg gttactccac tttgggtagg attgttctta aaaacaactt caacacagtt
     6241 tgacatgagt gccatttacc tcaaattgtt gttagaaatt gtggtgcctg tgagccttgg
     6301 attactattg caacgttttt ggggagcatt tgcacaacaa catttacgtg ccatcacttt
     6361 gtttgataaa tcggtaattt tgctgattgt ttacaaaagt tttgtagcat cgtttgaaga
     6421 aaatgttttt tcgacagtta gctggaccac atttgtactc gttacactag ttgttattct
     6481 ttccttcttt ttggtgtatg gattgattgg attgatttcc aaactcttgc attttaatcg
     6541 agaagatacg attgttgcgc aattttgtgg aacaaaaaaa tcattggtgc acggaacggt
     6601 tttttctaaa gtattgtttc caagcacctt tccagttggt attattttac tgccactcat
     6661 gctttttcac gccattcaat tgctggtggt gagtattatt gcaacaagga aataaacatc
     6721 aaagcaaatt ctatttttgg ttgcgcaaaa ttattacacg tatattattc aatgaaaatt
     6781 atacgtataa caataagatt ttgtattttt gatataaaat tagtcagcat gaacaccaaa
     6841 cttacattaa ccattgacca atccatcatt gagaaggcta aaaaatatgc acgaaaaaaa
     6901 gagcgtagtc tttctgattt gattgaaaat tccttgaaag ctttaacaaa tgatgatagc
     6961 tcgcaaacag agcaagaact atcgcctacc ttaaaatcgt taaagggttc gtttaaagag
     7021 ccaaagaatt ttgattacaa aaaagagctt acagaccgtt tatccaaaaa atatttagaa
     7081 tgaccaaagt actaattgac accgatgtta ttttagattt tttgtttgat agaaaaccct
     7141 attctgacaa tgccaccaaa atattggctt tgtgcgaaag tggtgcaatc aatggttttg
     7201 taacgtcaat aatgctgagc aacatttatt atttacttcg taaaactgca agtcacgtta
     7261 aggtaattga aagtttaaaa atgcttctac aatttttaga agtgcaaacc accgacaaaa
     7321 aagcggtatt agctgctttg aaatcggatt ttaaagattt tgaagatgca ctccaatatt
     7381 tctcagccca acaagactcc gagattagtg tgataatcac cagaaatgta aaagactaca
     7441 aaacgagtaa attagccatt atgccaccag aaatttactt gaaaagtagt gcgagataga
     7501 tttgtaattg ttaataattg aaacaaaact acaatcccat caattcttca gaagcggcct
     7561 cccatgcctg catttgctcg tctagttgcg attttaattg tgcataatcg gcaaggattt
     7621 tttgtgcttt tgcttcgtca gtgtagtcca aatcggccat tacaacatcc atctctgcaa
     7681 ttttagcttc caaacgttct atttcttttt ctgcaaaatt gattttattg ctcaacgcat
     7741 ttttttgttt tttgagctct ttttgttgtt ctctgtccaa ctcagtatta actggtgttt
     7801 ccaccttcac cgctggttct tctttctttg ctggtttttt caaactatct tttgcattgg
     7861 ccaaattcat ttttcgttcc aagaattctt taacgccgaa gtggtgaatt ttcaatgttt
     7921 tatcttcaat gtcccaaatt ctattggtca aatcgtccaa aaattcacgg tcgtgcgaca
     7981 ccacaataaa tgtaccttcg tagtttttaa gtgcttgttt taaaacttcc ttgctttgaa
     8041 tatccaaatg gttggtaggc tcatcgagaa taaggaaatt ggatggactt aaaagcaact
     8101 gacacaaggc caagcgcgtt ctttcgcctc ccgataaaac gcccacttgt ttctccacat
     8161 catcgccact aaaaagaaaa gttcctaaaa tagtgcgtaa gtttttgcga atttcacctt
     8221 cggcaacatc gtccaccgtt tgaaaaactg tttttgtttt atcgagcatt tctgcttgat
     8281 tttgagcaaa ataggtgata tttacattgt ggccatagtt aacggttcct tcaccatcta
     8341 attgtcccat gatgcgtttt aacaaggtgg atttacccac accgttaggt cccaataagg
     8401 caattttttc acctctaccc acagttaggt tgatgtcttt gaacaatagt ttttggtcaa
     8461 aagtcttccc catatcaatc atttccaaca cccattttcc aggttgaacg ctcaatggga
     8521 aagttacgtg cattttggta acattgtcat tgtccacctc tatgcgttcg gttttttcca
     8581 attttttgat aagcgattgc gcaaaagcgg ctttattggc ctttgcacgg tatttatcaa
     8641 tcaattcttg tgtttgctta atttctttat cttgctgttt tttagcagcg ttcatgcgtt
     8701 caatttcctc ggcacgcact tgtttgtatt tggtgtaggg atatggaaaa tcgagcactc
     8761 taccgttgtt gatttccaaa gtacgtttgg ttacattgtc caaaaataaa cggtcgtgcg
     8821 agattacaat tacagcgcct tcaaacgttt ttaagtaatt ttccaaccaa gcaatggaaa
     8881 taatatccaa gtggttggta ggctcatcca gcaaaataac atcggggttg ttgaccaata
     8941 ttttcgccaa ttcggcacgc attttccaac ctccagaaaa agtgttgagc gaacgagaaa
     9001 attcttcctc ctcaaaacca agtcctttta aagtattggc aattttttct tcccacgcat
     9061 aaccatcaat gatgttaaat tcatggttgc actcgttcaa acgatccagc aatcccaaat
     9121 aactgtctga ttcataatcg gttcttgata ccaattgatg attgatatcg tctaattcat
     9181 tgcgcagctt tgttaacttc tcgttggaat tattcaaata ttcaaaaaca gattgagtgg
     9241 tatcaatcac aatgtcttgc gtcaaaaatc cgatggtgca atcttttggt ttgtgaattt
     9301 taccctcaga tggttgtatc attcctgata aaatacgcaa cattgtcgat tttccggcac
     9361 catttttacc caccaaacca actttatcgt ttttattgat ttgtaaacca atattttgaa
     9421 aaagataacc ttgtggtaaa tgcaatccta gattttcgaa attaatcatt gtaaattgtt
     9481 ctgttttttg aaataaagcg caaaattaca gttttttagc tgattaatta actatcaact
     9541 gcaaatactt gttaaaccca actaaaattt gaaataagcg ggagtggata ttcaatttta
     9601 aatgcatttt atgaatgaaa tcgaaaataa tttgtaattt tcacacaatc aaacttcgta
     9661 gaaatgaaaa cgttactttt tattgttgct gttttggcaa cttttcaact ttcggcgcaa
     9721 agctattcaa gagttaaaat ttataccgat caaaatggaa ttgcacaatt gctcaatgca
     9781 ggtgttgccg tggatcatgg gattcgtaaa gaaggcgttt ttattatttc cgaattggcg
     9841 caacgtgata ttcaaaaatt gaacgaattg catttcaact acgaggtttt aattgacgat
     9901 gtatctcaat tttatgtgga gcaaaataaa acggcaaatc aaatagccaa aaacacaact
     9961 tgctcagcat caacaggagt tcctggtcaa accaacattc ccaccaattt tgatgtgcaa
    10021 tcaacctatg gcggttttta taaatacaac caaatgttgc aagaattgga cgatatggca
    10081 gcgttgtatc ccaatttaat ttcaacaaaa gcgcaaatag atacgttttt aacttgggaa
    10141 aacagaccga tttactacgt aaagatttcc aacaacccaa caatagataa cggtaaacca
    10201 atggtgttat attcagcaat acaccatgca cgcgagccaa ttggaatgtc agaagtgcta
    10261 tactacatgt ggtatttgtt agaaaattat gcaaccaatc cagaggtaaa atttttggta
    10321 gacaatacgc aaatgatttt tgttccgtgc atcaatccag atggttatat tcacaacgaa
    10381 acaaccaatc ccaatggtgg tggaatgcac cgaaaaaata aaaatccaaa cgtgggttct
    10441 tctaatcctg gtgtagactt gaatagaaat tacggttatc agtggaacac aacaggagta
    10501 agtgctgatg aagataacga cacttatcct ggaagcagtg ctttttcaga accagaaacc
    10561 catgccatgc gttggttggt acaaaacatg cccatccgtg cagcattgaa cgcccattca
    10621 tacagtaatt tgttgttgta tccacttgga aatgctactt cagcttttgc gcctcaccac
    10681 acttattttc aagcattgtc agaatacatg gtgcaataca acggctacac cgcacaaaaa
    10741 agcagtgcac tttatcctgc ttcgggcgat tcggatgatt acatgtacat catggataac
    10801 ggcgtgttag aaaaagacac catttttgcc atgacccccg aaattggtag tagtttctgg
    10861 cctgcaagtt ctgagatcgt tccattgtgc caaggaatgt tgtttgttaa ccttagcctt
    10921 gctcacatgg tgcaccgttt gtatgtggtt acagaaaatg atcctgcaac aatagccact
    10981 acaacaggat attttcacca caccgtgaaa aaattaggaa gagaaaacgg tagtgctacg
    11041 gtttccattg aaccattgct caacattaca actgttggag cgccagtggt ttacaatttg
    11101 gcaacattgg gcagtggaaa tgattctatt gcttttactt taaacagctc aattcaagca
    11161 ggagatacta ttcaatatgt gttaactact gacgacggag aatggataaa acgcgatacc
    11221 atcgtcaaac tatttggtgg agcaggcgat acgttgattt ttgaaaacgg tgatgcattg
    11281 accaattgga caggtgggtg gactacttcc tctaccattt attattcacc gtctaaatcc
    11341 attaccgatt cgccaggaaa ttatgccaat aacaccacaa aaatcatcaa ttatgcaaca
    11401 gccattgatt tgacagatgc aacagatgca agtgcttctt tttatgcgcg ttgggatatt
    11461 gaaaaagaat acgattattg ccagtttcaa gtttctaccg ataatggcgc aacttgggtg
    11521 gctcaatgtg gaaattatac ggggttttct accaatcaaa atggaacggt tcaacctcaa
    11581 aacgaacctt tgtgggacgg aacaaaaaca tcatgggtag aagaacacat caatttaagt
    11641 gattatttag ggcaaacact acaaattcgt tttattcttc aatcggatgg tggtgtgcgc
    11701 aaagatggtt tttactttga cgattttaaa attattgccg cattggatac cactacaaca
    11761 ggtaatgcag gattgaacaa ccttcaatcg gctataatta ccgtttcgcc caatccaagc
    11821 aatgacaacc taaccgttaa aaatgcacaa gcaaacgatg ctattttgct ctatgacttg
    11881 caaggaaaat tggttattgc aacccaacaa caaggtgaaa acccaacaaa agtggatgtt
    11941 tcatcgcttg aaaatggcgt gtatttgcta aaagtggaag gaacaaccag taattttatt
    12001 gaacaagtgg ttgttgcaca ttaagagggg aaatgagtag gcaaattgct tactcaaaac
    12061 tcaacccttg ttttcttaaa agttttacca cataaaacgc ataaaacgct aagtaggcaa
    12121 aacatacaat tccaacaata aacgatgatt ggatggagaa aatatcggca aatttacctt
    12181 gcaatggtgg aatgattgcg cctcctaaaa tcatcattac taaaaatgca gaaccctgtg
    12241 tttggtattt tcctaatcca cctagtgcca atgaaaaaat acatggccac atgatggaac
    12301 aaaacaaacc gccactcaaa aaggcataaa ttgcaaccgt tccacttgaa aaaataccaa
    12361 tggtaatggc caatgcgccc aaaattccaa aaacacccaa agtcaatgca ggtttatcgt
    12421 tggtgaggta aaacgccaca atttgaatgg caacacagat gatgtaccaa tgcaaatgct
    12481 ctaagtcgta gccaattaaa taaccagcac ccaaaacgac aaaaaaggca agaaacggaa
    12541 cgatgaagcg caaaatgttt ttcatgcgcg agctgatatc aaatgcagca acagcgccgg
    12601 tccagcgacc aatcatcaaa ctaccccaat acattgaaat gtagggagaa atttcggatg
    12661 ccgacatgtt ttggttgatt ttcaagtatt cgcctaaatt gctaccaatg gcaacttcaa
    12721 cgcccacgta gatgaaaatt gccaacattc ctaaggttaa ttgcggatat tgcattgctc
    12781 cccagttgat tttttctttt gaacctttga aaaatgctgt taacaaccca acaataacaa
    12841 ccaataaacc ggccaaccag ccaatgatac gaatttgttc tgcttcttct gcgtgaccag
    12901 aaaaacgctc gctgtagcta taaaaaacag gtgcaaaaca aatgatgagc aatcctgtga
    12961 ttaccaacaa ggtagtgat
//
LOCUS       DAOQVQ010000015        12937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5437_length_12937_cov_3.655100, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000015 DAOQVQ010000000
VERSION     DAOQVQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_12937_cov_3.655100"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKN22_01030"
     CDS             complement(<1..187)
                     /locus_tag="PKN22_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNS41314.1"
                     /translation="MKKTTESDSLYNDLEKQSTLELLTNINREDQKVAHAVALELPNI
                     EKFVDACFERMKNGGRLF"
     gene            complement(190..687)
                     /locus_tag="PKN22_01035"
     CDS             complement(190..687)
                     /locus_tag="PKN22_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNS41315.1"
                     /translation="MKQVILNHTQIEQKINRLAFQIIENTFELDTIYIAGITGNGIIL
                     AEKLKAIIENNSTQKIVLFEIILDKDKPLSHSIEMTIDSKELEGKTVILIDDVLNSGK
                     TMQYGLMKILEQPVHKIYTVVLVDRKHRRFPIKANFFGLRLTTTLHNHVEVSLEEGNY
                     SAQLS"
     gene            799..1800
                     /locus_tag="PKN22_01040"
     CDS             799..1800
                     /locus_tag="PKN22_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNS41316.1"
                     /translation="MKILIVRFSSIGDIVLTTPVVRAVKQQLNTTEIHFITKKSFASL
                     LANNPHISKLYTIEKSVTEVLDELKAEKYDVVIDLHKNIRTLRLKQALGVKSYAFDKL
                     NFQKWLLVNFKVDKLPNVHIVERYFEAVKEIGVVNDGKNCELFFDETAKVDTLKSFDL
                     QPKSFVAVAVGAQFATKVMPEKLLIEVLKKVEHPIVLLGGPTDVDRARAIEIGLQQHS
                     QKVINAVNQFSLAQSASIVAQSQVLLTHDTGLMHIATCYNTPIVSVWGNTVPAFGMYP
                     YYPSNTELFSIHEVLNLNCRPCSKIGYESCPKKHFNCMNLQDVAGIAQAVNQRFDLC"
     gene            1794..2471
                     /locus_tag="PKN22_01045"
     CDS             1794..2471
                     /locus_tag="PKN22_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNS41317.1"
                     /translation="MLEILFQDDYLVAINKPHGLLVHQSPIARNAEEFAVQLVRDQVG
                     KRVNPLHRLDRKTSGVLLFAFEKESTRLVREQFENNTVIKKYCALVRGFTPDAMTIDY
                     PLKNEKEVVQEAITHFKTLHRFEVDLPHGKHATSRYSLVEATPETGRMHQLRKHFAHI
                     LHPIIGDRPHGCNKQNKLWLEKYQMNTMLLHAELLEFLHPISHERIRIEAPKSGEFER
                     VLNILSK"
     gene            2579..3055
                     /gene="rimP"
                     /locus_tag="PKN22_01050"
     CDS             2579..3055
                     /gene="rimP"
                     /locus_tag="PKN22_01050"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNS41318.1"
                     /translation="MINKKRVEELINERIAELNNGLFVVELSISASNVITVELDKHQG
                     GVSVDDCIAVSRNIEHNLDREQQDFELNVSSAGLDKPLRVLPQFIKNIGRTVKVVKMD
                     GTKLEGELKSATETGIVVENSRTEKVEGKKKKEVIVEQHQLPFDNIKETKIVITFK"
     gene            3062..4297
                     /gene="nusA"
                     /locus_tag="PKN22_01055"
     CDS             3062..4297
                     /gene="nusA"
                     /locus_tag="PKN22_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNS41319.1"
                     /translation="MNSLELVESFSEFKELKSIDKQTMQHVLEDVFRSMLKKKFNTDE
                     HIDIVVNIDKGDVQIFLNKEIVDDGEVEDPNMEIAYSDAIKIEPDFEIGEEVTEEFKF
                     EDFGRRNILSLRQNLISRILELEKDNLYKVYKSRIGEIITGEVYQTWKKEILVLDDEG
                     NELILPKSEQIPSDFFKKGEAIRAVVYKVDMRNSQPTIILSRTAPEFLERLFELEVPE
                     VFDGLITIKRIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INFTTNPQLFIQRALSPAKISSIEIEEEEKRARVYLKPDQVSLAIGRGGHNIKLAGKL
                     TGYELDVYREGEVDIDDVDLDEFVDEIDGWILDELKAIGCDTAKSVLELSVQDLVQRT
                     DLEEETIEEVLKILRSEFE"
     gene            4362..7139
                     /gene="infB"
                     /locus_tag="PKN22_01060"
     CDS             4362..7139
                     /gene="infB"
                     /locus_tag="PKN22_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNS41320.1"
                     /translation="MAGKPIRLGKAAGELNVGISTLVEFLDSKGVKIENNPNFKLEEE
                     HFNLLRQEFAADHDLKEQTKQNPIKREKRESVSLKDTREVEAPSHDDDEDDEVELPIK
                     ETPKVVVPEPPVAKVEITEETPVVDDNSPVKVIGKIDLDALNTKTRPDKKKKEDEVAP
                     IKEEPKVETKVEPKIEEEKVVAPVEEEKIPEIVEKVEDQPAEEPEEIETIRVQSQTLS
                     GPKILGKIELPVEKPKSNDKGGDKNADKKKRKRIKKIDVNKQAQQQQQGQNQQQGQNK
                     GNNKFKKGGAPIERPEISQQDIEKEIKDTLARLSSKGAKSKGAKNRKAKRDVFAQRRQ
                     EEELAAELDSKILKLTEYVTVSELANMMNVSPTQVISACMSLGIFASINQRLDAETIQ
                     IVADEFGFDTEFVSAEVQDAIPVVEDKEEDLIPRPPIITVMGHVDHGKTSLLDKIRNA
                     HVTAGEAGGITQHIGAYSVVKDGKKLTFLDTPGHEAFTAMRARGAQVTDVAIIIVAAD
                     DDVMPQTKEAISHAQAAGVPMVFAINKIDKPGANPDKIREQLSQMNILVEEWGGPYQS
                     QEISAKQNLNIDALLEKVLLEADILNLRANPNKNALGVVIESSLDKGKGYVTNMLVES
                     GTLRVGDVVLVGRYYGKVRAMHDENGKSLKEAGPATPVSILGIGGAPNAGDKFNVMDD
                     EREAKTIASKREQLYREQGLRATKHITLDEIGRRLAIGDFKELNIIVKGDVDGSVEAL
                     SDSLLKLSTEEIQVNIIHKGVGAISEADVNLASASDAIIIGFQVRPVVSARKLAENEQ
                     IDIRLYSVIYKAIEEIKAAMEGMLSPDIEEQVIGSAEIRETFNISKVGTIAGCYVLDG
                     LIKKSAKIRLIRDGIVIHTGTLGSLKRFKDDVREVKNNYECGLNIDKFNDIQVGDIIE
                     AFEEVEVARKL"
     gene            complement(7197..10814)
                     /locus_tag="PKN22_01065"
     CDS             complement(7197..10814)
                     /locus_tag="PKN22_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS41321.1"
                     /translation="MKKIISKYSSWLTILIVGVAFLVLYFQLFSTNHYRELNKVRTNF
                     KQLENSLQQHKIKVDNAINSNQKFDEIALKKAINLHVYKQDTLIYWSTNKIPVSKFED
                     IQFPANGLIKAQNGWYYAQSYQKGAYSYTLSFLIKNEYSYVNEYLENDFSNELNPKVK
                     SSISLDESVGFPIYNHDKKYVFSILVNEYQPISLVDTIVLFCLMILWLSLIFLKITQL
                     VLRIESHLIWLYPVTLICIRYCSLHFEWFGFFRNSELYSPSLYGTNELFPNFLEYNIN
                     ILTLFIVSYFVYKRLGTSKLKINKFWFYIVCALPYIVWQILLFFFQGLIENSSIPLHI
                     EELFKINVYSIIAIISMAIIGFMYFLINKTIVRLIKNNFYSLNKFFTIQLIFGISYVI
                     AHQFYINNETLATSFFPLLFVLSIALLEYKEFNQKHLILGMIMLALYAFANAHIINDL
                     SQKRSKSEKELYANQLLIEKDIVTELEYSNLVKSIRNDRFLQQMISAGFQLNFREFER
                     AMERRHFNGFWERYECKFFLFNVNGGSLLNLQGNSDDLLNNLNDYVNNHGIQSEIVPS
                     IHYISDYIGQYSYIIREQINGRNGELATLFVTLKSKKIPEEIGYPRLLISSESTALHH
                     LDNYSIAKYHNNRLVSQFGSYNYPNSLRGLRKDNFKHLDNFNLNGYNHLIQFKSKNDV
                     VVLSNINITWLDTITSFSYLFCFFGLLLLPIYFRYYSTSFTFNSLTLSTKIQFTLIAI
                     VVVALVVSAISSGIFIKNQYSSFSEISLKEKVHSVEDELSPLINKEKKLTIQENGSFI
                     NYQLIGISKIFNTDINVYDRNGFLISTSRPNVFNSGLLSEQINPIAKKELILMDKSEF
                     IHTENIGSLNYNSAYAPLYNKLGKTIGFINLQHFGQQEEVEHQIQQFLMSIISVFVLL
                     IAGSVIIAIFAASWITNPLHMLQSYLSNIHLGKSNQYINYSKNDEIGALVKSYNKKID
                     ELEYAAQQLAQSERESAWRDMAKQVAHEIKNPLTPMKLSVQHLVRSFDPNDENAQNKI
                     NRVCQSLIEQIDALTKIANEFSNFAKMQKAEFNAIDIVSLIRNAAELFSEGSQFEIII
                     TGPEQCIVNGDKDQLLRVFNNLIKNSCQAISNKTDGKIVIEILKTATLIKISVNDNGV
                     GIPEIQQSKIFVPYFTTKSNGSGIGLAMVKQIIENHKGNIYFESEVNKGTTFIIELPL
                     L"
     gene            complement(10818..11366)
                     /locus_tag="PKN22_01070"
     CDS             complement(10818..11366)
                     /locus_tag="PKN22_01070"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNS41322.1"
                     /translation="MQFLEQKSIHGTMPLNFEENTPESLQTLINALTGKSYKIEIDSC
                     QKNQKGSANGELIGGNLSIIYSLIGTNCQPSYKNKILFIEEVGEQLYALDRMLFSLKK
                     AGILDEISGLIIGGMTNMKDSTEVSIGMTIEDIVLQHFTYKKIPICFNFPSGHLDDNR
                     ALILGENVYLKIDSKVCLEFKL"
     gene            complement(11370..11717)
                     /locus_tag="PKN22_01075"
     CDS             complement(11370..11717)
                     /locus_tag="PKN22_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNS41323.1"
                     /translation="MNKIPYLQKGDLIEIVAPAKAIESKYVEFAKNFLEQEGFLVKIS
                     GNCLGRHNYFSGTDAQRLHDFQAALDNPEVKAILCARGGYGAVRIVDAIKWANQLIHP
                     KWIVGFSDITVFH"
     gene            complement(11752..12111)
                     /locus_tag="PKN22_01080"
     CDS             complement(11752..12111)
                     /locus_tag="PKN22_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNS41324.1"
                     /translation="MTHIELGQHGEEMAVSYLRNNDYEIIERNFRFKKLEIDIVALKN
                     GQLIVCEVKTRVTAEIGEPFLAVTKSKQKQIIKATNHFLISRNLNINVQFDIISIVHN
                     SIRTKIEHIENAFFPLL"
     gene            complement(12206..>12937)
                     /gene="recA"
                     /locus_tag="PKN22_01085"
     CDS             complement(12206..>12937)
                     /gene="recA"
                     /locus_tag="PKN22_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNS41325.1"
                     /translation="HAFDRFYAEKLGVDIDNLLISQPDNGEQALEIADNLIRSGAIDL
                     LVVDSVAALTPKAEIEGEMGDSQMGLQARLMSKALRKLTGSINKAKCCCIFINQLRDK
                     IGVMFGNPETTTGGNALKFYASVRVDIRKTSQIKDGEDVIGNRVKVKVVKNKVAPPFK
                     KAEFDIMYGEGISKIGEIIDLGVELNIIKKSGSWFSYGETKLGQGRDAVKALLNDNIE
                     LMEELENKIKSALGTTESVEAVIQD"
BASE COUNT         4378 a         2037 c         2333 g         4189 t
ORIGIN      
        1 aaaacaaacg tcctccattt ttcatacgct caaaacatgc gtctacaaat ttttcaatgt
       61 tgggcaattc caatgcaacg gcatgtgcca ctttttgatc ctcacgattg atgttggtta
      121 ataattccag tgtggattgt ttttccaaat cattgtagag cgagtcggat tctgttgttt
      181 ttttcatggt tatgacaatt gagcagaata gtttccttct tccaatgata cttcaacgtg
      241 attgtgcaat gttgttgtta atcgcaaccc aaagaaattg gcttttattg gaaaacgacg
      301 gtgttttcta tccaccaaaa caacggtgta gattttatga acaggttgct ctaaaatttt
      361 cattaatccg tattgcattg ttttaccaga gttcaaaaca tcgtctatca atataaccgt
      421 tttgccttct aattctttag aatcaattgt catttcaatc gaatgcgata acggtttgtc
      481 tttatccaaa attatttcaa aaagcactat tttttgagta gagttattct caataatggc
      541 ttttagtttt tccgctagaa tgattccatt gcccgtaatt cctgcaatgt aaatagtatc
      601 aagttcaaat gtattttcaa ttatttggaa tgccaaacgg ttgatttttt gctcaatttg
      661 ggtgtgattt agaataactt gtttcattga taacggattt ttcacaaaga taataaaacc
      721 ttgtttggtt gattttggat ttttgataaa ttgcacaagt aattcaattt tttgctacta
      781 tttcattact tttgaaccgt gaaaattttg attgttcgtt tcagctctat tggtgatatc
      841 gtacttacaa cacccgttgt tcgtgctgtg aaacaacaat tgaatactac cgaaattcat
      901 tttatcacca aaaaatcatt tgcttctttg ttggcaaata acccacatat ttcaaagtta
      961 tatactattg agaagtcagt gacagaagtg ttggacgaac ttaaagctga aaaatacgac
     1021 gttgttatag acttgcacaa aaacattcga acattgcgtt tgaagcaagc tttgggtgtt
     1081 aaaagttatg cgtttgacaa attgaatttt caaaaatggt tgttggttaa ttttaaggtg
     1141 gataaacttc ccaatgtgca cattgttgaa cgctattttg aagctgtcaa agaaattgga
     1201 gttgtcaacg atggcaaaaa ctgcgaatta ttttttgatg aaacagcaaa ggttgatacc
     1261 ctaaaatcgt ttgatttaca accaaaatct tttgtagcag tggcggttgg agcacaattt
     1321 gcaaccaaag taatgcccga gaaattgttg attgaggtat tgaaaaaagt ggaacatccc
     1381 attgttttgt taggtgggcc tacagatgtt gaccgcgcac gagcaattga aatcggtttg
     1441 caacagcatt ctcaaaaagt aatcaatgca gttaatcaat tttctttagc ccaatcggca
     1501 tctattgttg ctcaatcaca agtgttattg acccacgata cagggttaat gcacatagct
     1561 acgtgctaca atacccccat tgttagtgtg tgggggaata ccgttccagc ttttggaatg
     1621 tatccctatt atccttccaa taccgaattg tttagcattc acgaagtgtt gaatcttaat
     1681 tgtcgccctt gctctaaaat tggatacgaa tcgtgcccta aaaaacattt caattgcatg
     1741 aatttgcaag atgtagctgg aattgcacaa gcagttaatc aacgatttga cctgtgttag
     1801 aaattttgtt tcaagacgat tatttggtgg ccatcaacaa gccacatgga ttattggttc
     1861 atcaatcgcc cattgcccga aatgccgaag aatttgctgt tcaattagta cgtgatcagg
     1921 ttggaaagcg tgtcaatccc ttgcaccgat tggataggaa aacaagtgga gttttgcttt
     1981 ttgcctttga aaaagaaagt actcgtttgg tgcgtgaaca gtttgaaaac aacaccgtta
     2041 tcaaaaaata ttgtgcattg gtgcgtggat ttacacccga tgcaatgacc attgattatc
     2101 cgttgaaaaa cgaaaaagag gtggtgcaag aagcgattac tcattttaaa accttacacc
     2161 gttttgaagt ggatttacca catggtaaac atgcaacttc gcgttattct ttggttgaag
     2221 caacaccaga aactggaaga atgcatcaat tgcgcaagca ttttgcacat attttacacc
     2281 ccattattgg tgatagacca catggatgca ataaacaaaa caaattgtgg ttggaaaaat
     2341 atcagatgaa taccatgttg ttgcatgctg aattgttgga gtttttacat ccaatatcgc
     2401 atgaaagaat tcgaattgag gcaccaaaaa gtggtgaatt tgaacgtgtt ttgaatattt
     2461 tatccaaata aaaatgaaaa aaaagtagtt ttattcaaat aattccgtat atttgcaaca
     2521 catttaagaa caaaatcaag aatgagggga cgaaagtccc ctctttttat aaccaacaat
     2581 gataaacaag aaaagagtag aagagttgat taacgagcgc attgcagaat tgaacaatgg
     2641 tttgtttgtg gtagagttat ctatttctgc tagtaatgta attacggtgg agttggataa
     2701 gcaccaagga ggcgtttctg ttgatgattg cattgctgtg tcgcgcaata ttgagcacaa
     2761 tttggataga gaacagcaag attttgaatt aaatgtttct tctgcaggtt tagacaaacc
     2821 gttgcgtgtt ttgcctcagt ttatcaaaaa cattggtaga accgtgaaag ttgtaaaaat
     2881 ggacggtaca aagttggagg gagagttgaa atcggcaaca gaaaccggaa ttgttgtaga
     2941 aaactctaga accgaaaaag tagaagggaa aaagaaaaaa gaagttattg tagagcaaca
     3001 tcaattgcca ttcgataata taaaagaaac gaaaatagtt attacattta aataggtcac
     3061 tatgaatagc cttgaattag tagaatcgtt ctctgagttt aaagagttaa aaagcatcga
     3121 caaacaaacg atgcaacacg ttttagaaga cgtgtttcgt agcatgttga agaaaaaatt
     3181 caacacagat gaacacatcg atatcgttgt aaatatcgat aaaggagacg ttcaaatatt
     3241 tttgaataaa gaaattgtag acgatggaga agtagaagat ccaaacatgg agattgccta
     3301 ttcggatgca attaaaattg aaccagattt tgaaattggt gaagaagtaa cagaagaatt
     3361 taagtttgag gactttggtc gcagaaacat tctttctttg cgtcagaact tgatttcaag
     3421 aatcttggaa ttggaaaaag acaatttata caaagtttac aaaagccgta ttggagaaat
     3481 tattacggga gaagtttatc aaacttggaa aaaagaaatt ttggtattgg acgacgaagg
     3541 taatgaattg attttgccta aatcagagca aattccttct gatttcttca aaaaaggaga
     3601 agctattcgt gcagttgttt acaaagtaga tatgcgcaat tctcaaccaa caattattct
     3661 ttctagaaca gcacctgaat tcttggagcg tttgtttgaa ttggaagttc cagaagtatt
     3721 tgatggcttg attaccatta agcgtattgt tcgtgaacca ggagaaagag caaaagttgc
     3781 ggttgaatca tacgatgacc gcattgatcc agttggagct tgtgttggta tgaaaggttc
     3841 tcgtattcat ggaattgttc gtgagttaag aaatgaaaat attgacgtaa ttaactttac
     3901 aaccaatcct caattgttta ttcaaagagc gctttcacca gcaaaaattt catctattga
     3961 aattgaagaa gaagagaaaa gagcgcgtgt ttatttaaaa ccagatcaag tttctttagc
     4021 tatcggtaga ggaggtcaca atatcaaatt ggctggaaaa ttaacaggat acgaattaga
     4081 tgtttaccgt gaaggcgaag tagatattga tgatgttgat ttagatgaat tcgttgatga
     4141 aattgatggc tggattttgg atgaattgaa ggcaattggt tgtgatacag cgaaatctgt
     4201 gttagaattg agtgttcaag atttagttca acgaacagac ttagaagaag aaacaatcga
     4261 agaagtatta aaaatcctac gttcagagtt cgaataatcg gattcatgta gtatatttag
     4321 gcacgattta taaaaaaaca aattaaaagg acgcacaatt tatggcggga aaaccaataa
     4381 gattaggaaa agcagcaggt gagctaaatg tgggaatttc cacattggtt gagtttttgg
     4441 actcaaaagg cgttaaaatt gaaaataatc ccaatttcaa attggaggag gagcatttta
     4501 acctattgag acaagaattc gctgctgacc atgacttaaa ggaacaaaca aaacagaatc
     4561 cgattaaacg cgaaaaaaga gagtccgttt cattgaagga tacacgtgaa gtagaagctc
     4621 caagccatga tgacgatgag gacgatgaag tagaacttcc aattaaggaa actccaaaag
     4681 ttgttgttcc agaacctcct gtagctaaag ttgaaataac cgaagaaaca ccggttgttg
     4741 atgacaatag tcctgtgaaa gttattggaa aaatcgacct tgacgcattg aacacaaaaa
     4801 cgcgtccgga taaaaagaaa aaagaagacg aggttgctcc tattaaagaa gaaccaaagg
     4861 ttgaaactaa agttgagcct aagattgaag aagaaaaagt tgttgcacct gtagaagaag
     4921 agaaaattcc agaaattgtt gagaaggttg aagaccaacc agcagaagag ccggaagaaa
     4981 tcgaaacaat tcgtgtgcaa agtcaaactt tgtctggacc taaaattttg ggtaaaattg
     5041 aattaccagt tgaaaagcca aaatccaatg ataaaggagg cgataaaaat gctgacaaaa
     5101 agaaaagaaa acgcatcaag aaaattgatg taaacaaaca agctcagcaa cagcaacaag
     5161 gtcaaaacca acaacaaggt caaaacaaag ggaacaataa gtttaaaaaa ggcggtgctc
     5221 cgattgaacg ccctgagatt tctcaacaag atattgaaaa agaaatcaaa gatacattag
     5281 ctcgtttgtc aagcaaaggt gctaaatcaa aaggtgccaa aaacagaaaa gcaaaacgtg
     5341 atgtttttgc acaacgccgt caagaggaag agttagcagc agaattagac agtaaaatat
     5401 tgaaattaac ggaatatgtt actgtttctg agttagcaaa catgatgaat gtttcaccga
     5461 cacaagttat ttctgcttgt atgtcattgg gtatttttgc ttctatcaac caacgattgg
     5521 atgctgaaac tattcaaatt gtagcagatg aatttggttt tgatactgaa tttgtaagtg
     5581 cagaagtaca ggatgcaatt ccagtggttg aagataaaga agaagattta attccacgtc
     5641 caccaattat tacggtaatg ggacacgttg accatggtaa aacatccttg ttggataaaa
     5701 ttcgtaatgc acatgttaca gcgggtgagg caggtggaat tacacagcac attggggctt
     5761 attcggttgt gaaagatggc aaaaagctta cgttcttaga tactccaggt cacgaagcct
     5821 ttacggcgat gcgtgcacgt ggtgctcaag ttacagacgt agcgattatc attgtagcag
     5881 cagatgatga tgtgatgcct caaacaaaag aagcgatttc acacgctcaa gcggctggtg
     5941 taccgatggt ttttgcaatt aataaaattg ataaacctgg tgcaaatcct gataaaatcc
     6001 gtgagcaatt atcgcaaatg aatattttgg tggaagagtg gggtggacct taccaatcac
     6061 aagaaatttc tgctaaacaa aacttgaata ttgatgcatt acttgaaaaa gtattgttag
     6121 aagctgatat attaaatcta cgtgctaacc cgaacaaaaa cgcattaggt gttgttattg
     6181 aatcatcttt agataaagga aaaggttatg taaccaatat gttggttgaa tctggtactt
     6241 tacgtgtagg cgatgtcgtt ttagtcggta gatattatgg taaagttcgt gcaatgcacg
     6301 atgaaaatgg taaatcttta aaagaagccg gcccagcaac gccagtatct attttaggta
     6361 ttggtggtgc cccaaatgcg ggtgataagt ttaacgtgat ggatgatgag cgtgaggcta
     6421 aaacaattgc atctaaacgc gaacagttgt atagagagca aggtttacgt gcaactaagc
     6481 atattacgtt ggatgaaatt ggacgtcgtt tggcaattgg tgactttaaa gaattgaaca
     6541 tcattgtgaa aggtgacgtg gatggttctg ttgaagcatt atctgactca ttattgaaat
     6601 tatcaactga agagattcaa gttaacatta ttcacaaagg tgtaggtgca atttctgaag
     6661 ccgatgtaaa cttagcttct gcatcggatg caattattat tggtttccaa gttagaccag
     6721 ttgtttcggc tcgtaaatta gcagaaaatg agcaaataga tattcgtttg tattctgtaa
     6781 tttacaaggc aattgaagag attaaagctg caatggaagg aatgttatct cctgatattg
     6841 aagaacaagt tattggttca gctgaaattc gtgaaacttt caatatttca aaagttggta
     6901 caattgcagg atgttacgta ttggatggac ttatcaaaaa gtcagctaaa atccgcttga
     6961 ttcgtgatgg tattgtaatt catacaggaa cattaggatc attgaaacgt ttcaaagacg
     7021 atgtgcgtga agtaaaaaac aactatgaat gtggtttgaa tattgataaa ttcaacgata
     7081 ttcaagtagg tgacatcatt gaggcatttg aagaagtaga agttgcaaga aaattgtaat
     7141 attctaaaga ttgaaataaa ccaaaaagct atctgaaaag gtagcttttt ttatacctat
     7201 aagaggggta gttcaataat gaatgtggtg cctttgttga cttccgattc aaaatatata
     7261 tttcctttgt ggttttctat aatttgtttc accattgcca atcctattcc agaaccgttt
     7321 gatttagtcg tgaaataggg cacaaatatt tttgattgct gaatttcagg aattccaact
     7381 ccattgtcgt ttacagaaat cttaattagt gtagctgtct ttagaatttc aataactatt
     7441 ttcccatctg ttttgttaga tatagcttgg caactgtttt ttatcaagtt gttaaaaaca
     7501 cgcaaaagct ggtctttgtc accattaaca atacattgct caggtccagt aattataatt
     7561 tcaaattggc ttccttcact gaataattcg gcagcattgc gtatcaatga aacgatgtct
     7621 attgcattga actctgcttt ttgcattttt gcaaagttgg aaaattcatt ggctattttt
     7681 gtcaatgcat caatttgttc aatcaaggat tggcaaacac ggttaatttt attctgggca
     7741 ttttcatcat ttggatcaaa agatcgcacc aaatgttgaa ctgataattt catgggtgtc
     7801 aatgggttct taatttcatg cgccacttgt ttggccatat cgcgccatgc cgattcacgt
     7861 tcgctttgag ctaattgctg ggctgcatat tctaattcat caattttttt gttataactt
     7921 tttacgagag caccaatctc atcatttttt gaataattaa tgtattgatt ggattttcca
     7981 aggtgaatat tggataaata gctttgaagc atgtgtaatg ggttcgtaat ccaacttgca
     8041 gcaaaaatgg ctataataac tgatcctgct attagtaaca caaaaacact tatgatagac
     8101 attaaaaatt gttggatttg atgttccact tcctcttgtt gaccaaagtg ctgtaaatta
     8161 atgaatccta tagttttacc tagtttgtta taaagcggag cataggcaga attgtagtta
     8221 agacttccaa tattttctgt atgaataaac tcacttttat ccattaatat taattctttt
     8281 tttgcgattg gattaatttg ttcactcaat aatccactat taaatacatt tggacgagag
     8341 gtactaatta aaaatccatt tctatcataa acatttatgt cggtattaaa tattttcgaa
     8401 attccaatta attgataatt gataaaagaa ccgttttctt gaatagttag ctttttttct
     8461 ttattaatca atggtgatag ttcatcttca acagaatgca ctttttcttt tagtgaaatt
     8521 tctgaaaagg aactgtattg atttttaata aaaattccac tacttatagc agaaacaacc
     8581 aaagcaacca ccacaattgc tattaatgta aattgtattt tagtggataa tgtcagtgaa
     8641 ttgaacgtaa aagaagttga gtaataacgg aaatatattg gcaataataa taaaccgaaa
     8701 aaacaaaaca gataggaaaa tgatgtaatg gtgtccagcc aagttatatt gatattagag
     8761 agtacaacaa catcattttt agatttgaat tgaataagat ggttgtatcc attcaaatta
     8821 aaattatcta aatgtttaaa attatctttt cttaatcctc ttaatgaatt gggatagttg
     8881 taacttccaa attgagatac caaacggttg ttatgatatt ttgcaataga ataattatct
     8941 aaatgatgta aagctgttga ttcagaagat attaaaagtc gtggataacc aatttcttct
     9001 ggaattttct tagattttaa cgtaacaaac aaagttgcta gttcgccatt tcttccgttt
     9061 atttgttcac gaatgatgta gctatactgg ccaatatagt ctgaaatata atggatagaa
     9121 ggtactattt ctgattgaat tccatgattg ttgacataat cattgaggtt gtttaataaa
     9181 tcatcagaat taccttgtaa gtttaagaga gagccaccat taacattaaa aaggaagaat
     9241 ttacattcat aacgttccca aaatccattg aaatggcgac gctccattgc tctttcaaat
     9301 tctctaaaat ttaattgaaa acctgcacta atcatttgtt gtaaaaaacg atcattgcgg
     9361 atagatttca ctaaattgga atattctagc tctgtaacaa tgtctttttc tatcaataat
     9421 tgattggcat acaattcttt ttcactctta cttctttttt gacttaaatc attgattata
     9481 tgtgcatttg caaaagcata aagggccagc attatcattc ccaatatcag atgcttttgg
     9541 ttaaattctt tgtattcaag caatgcgatt gaaagaacaa ataacaaagg gaaaaatgat
     9601 gttgctaatg tttcgttatt gatataaaat tgatgagcaa taacataaga aattccaaat
     9661 atcaactgta ttgtaaagaa tttattgagc gaataaaagt tgttttttat aagtctaaca
     9721 atggttttat taattaagaa atacataaaa ccaataattg ccatactaat tattgcaatg
     9781 attgaataca cgttgatttt aaacaattct tctatatgaa ggggaatact agagttttca
     9841 attaatcctt gaaagaaaaa aagtagtatt tgccacacaa tatatggcag agcacaaaca
     9901 atataaaacc aaaacttgtt gattttgagt ttagaagttc ccaatcgttt ataaacaaaa
     9961 tagctgacaa taaataaagt gaggatgttg atgttgtatt ctaaaaaatt ggggaataat
    10021 tcattagttc catacaaact tgggctatac aactcagaat ttctaaaaaa tccaaaccat
    10081 tcaaaatgca atgagcaata cctaatgcaa attaatgtta ctgggtacaa ccaaattaga
    10141 tgagattcaa ttcttagaac taactgtgtt attttaagaa aaataagtga taaccataga
    10201 atcattaagc aaaaaagtac aattgtatct actaatgaaa taggctgata ttcattgact
    10261 agaatagaaa atacatattt tttatcatgg ttatatattg gaaatccaac agattcatcc
    10321 aaagagatgg atgatttgac ttttgggttt agttcattcg aaaaatcatt ttctaaatat
    10381 tcgttcacat aagaatattc attttttata agaaatgaaa gtgtatatga ataagcaccc
    10441 ttttgatacg attgagcata ataccagcca ttttgtgctt ttattaagcc atttgctgga
    10501 aattgtatat cctcaaattt tgaaacagga attttattgg tgctccaata aattaacgtg
    10561 tcttgtttat atacatgtaa attaatagct ttttttaaag ctatttcatc aaatttttga
    10621 ttggaattta tcgcattatc aactttaatt ttatgttgct gtaacgaatt ttctagttgc
    10681 ttaaaattcg ttctaacttt atttaattca cgataatgat tggtagaaaa taattgaaag
    10741 taaagtacaa ggaaagccac tccaacgatt aggatggtaa gccaactact atattttgaa
    10801 attatttttt tcattttcta caatttaaac tctaaacata ctttagaatc aatttttagg
    10861 tacacatttt cacccaatat taaagctctg ttatcatcta aatgcccact tggaaaatta
    10921 aaacaaatgg gtattttttt gtaagtaaaa tgctgaagaa ctatatcttc aatcgtcatt
    10981 ccaatagata cttctgttga atctttcata ttggtcattc ccccaataat caaaccacta
    11041 atttcatcta aaataccagc ttttttcaac gaaaataaca ttctatccaa tgcatagagt
    11101 tgttcaccta cttcttcaat aaataagatt ttattcttat aagagggttg acaattggtt
    11161 ccaattaaac tataaataat gcttaaatta ccaccgatta attctccatt agcactccct
    11221 ttttgatttt tttggcaact atcaatttct attttataag actttccagt tagcgcattt
    11281 atcaatgttt gcaaagattc aggagtattc tcctcaaagt ttaaaggcat tgtaccatga
    11341 atgctttttt gttctaaaaa ttgcatttgc taatgaaaga ccgtaatatc actgaaacca
    11401 acgatccatt tagggtgaat caattgatta gcccatttta tagcatccac aattcgaaca
    11461 gcaccataac cgcctctagc acaaagtatt gctttaactt caggattgtc taatgcagct
    11521 tgaaaatcat gcagacgttg tgcatctgtt cccgagaaat aattgtgcct tcccaagcag
    11581 ttgccactaa ttttcactaa aaacccttct tgttccaaaa agtttttagc aaattcaaca
    11641 tatttacttt caattgcctt tgctggcgca acaatttcaa ttaaatcgcc tttttggagg
    11701 tatggaattt tattcatggt tgtattaata catcaaagaa acaaaatttc tttaaagtaa
    11761 cggaaaaaaa gcgttctcaa tatgttcaat ttttgttcga atactattgt gaacaattga
    11821 aattatatca aattgtacat taatattcaa atttctactt atcagaaaat gattagttgc
    11881 cttaataatt tgtttttgtt tgcttttggt tacagccaaa aaaggttctc caatttctgc
    11941 agttacgcgg gtttttactt cacaaacgat tagttgacca ttttttagtg caacaatatc
    12001 aatttcaagt tttttaaatc ggaagttgcg ttctatgatt tcataatcgt tgtttcttag
    12061 atagcttaca gccatttctt cgccatgctg gcccaattca atgtgtgtca taaaaaggta
    12121 gttttttaat tgtaaaagat ttagataatc gttaaaaaaa gcgagtatat ttcacctcgc
    12181 ttttacccaa cgatttacaa ctaaactaat cttgaattac agcttcaaca gattctgtag
    12241 ttccaagagc acttttaatt ttattttcta attcttccat tagttcaatg ttgtcattca
    12301 acaatgcttt cacggcatct cgaccttgtc ctaatttagt ttcaccataa gagaaccaag
    12361 atccactttt tttgataata tttaactcaa ctcccaagtc aatgatttct ccaatttttg
    12421 aaattccttc gccatacata atgtcgaatt ctgctttttt gaacggtgga gcaactttat
    12481 tttttacaac tttcacctta acgcggttac caataacatc ttcaccatct ttaatttggc
    12541 ttgttttacg tatatctaca cgtacagaag catagaattt caaagcatta cctcctgttg
    12601 tagtttccgg atttccaaac atcaccccaa ttttatcacg taattggtta atgaaaatac
    12661 aacaacattt tgctttgttg atagagcctg ttaattttct cagtgctttt gacatcaaac
    12721 gcgcttgtaa acccatttgc gaatcgccca tttctccttc aatctccgct tttggtgtta
    12781 atgcagcaac cgagtcaact accaataagt caattgctcc cgaacggatt aggttgtctg
    12841 caatttccaa cgcttgctca ccattatcag gctgtgaaat caataaattg tcaatatcaa
    12901 cacctaattt ctcagcataa aaacggtcaa atgcatg
//
LOCUS       DAOQVQ010000016        12576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5670_length_12576_cov_3.564651, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000016 DAOQVQ010000000
VERSION     DAOQVQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5670_length_12576_cov_3.564651"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..790)
                     /gene="mazG"
                     /locus_tag="PKN22_01090"
     CDS             complement(23..790)
                     /gene="mazG"
                     /locus_tag="PKN22_01090"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNS41326.1"
                     /translation="MDERLKAFNRLLDIMDDLREKCPWDKKQTFESLRHLTIEETYEL
                     ADAILDNDLKELEGEIGDIFLHMVFYSKLGSEVGAFDVSSVLNRICDKLVHRHPHIYG
                     DVQAETEEQVKANWEKLKLKEGKKSVLQGVPQSLPAMVKAARIQEKVKGVGFDWDNKE
                     QVWNKVQEELNEFKAEVDAQSPQIEDEFGDLLFSLINYSRFIGVNPENALEKTNKKFI
                     ARFMKMEELLHNNQQQMGELSLEELDVYWEQAKKILK"
     gene            852..1946
                     /locus_tag="PKN22_01095"
     CDS             852..1946
                     /locus_tag="PKN22_01095"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNS41327.1"
                     /translation="MRSVLICCLLALSSVSFAQEKINWGLEARMKYGFLIGHRVVMGH
                     IAQNHTYAAELAFVYRPNGEKAWHKYYKYPDLSINLYFGNVGNTKLLGNFGGIYGSLA
                     IPFVARKHFRLSGKLGCGLGYTNKVYDEVLNNKNSAISTRINALINLGIDTRTYFKNN
                     WILFGIDLTHFSNGGTKVPNLGINIPQITLGYGRFIHYNEKHSTIEKPTYIPQRRLLF
                     GATAIASAKQVFPTNGRTYPVFALNVHMRMFMKPRVGWEVAFDAMSNQSIFGYRPEVQ
                     KTQWKVMQFGVFAGYLLPLNNFHFVLGMGAYVRDFYKPEELFYHRVGMRYYLKSGVHF
                     NLVLKSHWGKADYVEWGVGYTFFNRKKDKK"
     gene            1943..2686
                     /locus_tag="PKN22_01100"
     CDS             1943..2686
                     /locus_tag="PKN22_01100"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNS41328.1"
                     /translation="MRFVALILIVSAFTACKKAEDRRCMKAVGDNVTLELALPEFDKL
                     RVGPKMEVVLVQDTVNKMIVNGGENLINFITAEMNNEGYLLLKNSNKCDFLRSYKKSL
                     IKVELHFKEIRNLFFEGTHDITTLGAITTNTFGLEIQDSGATAYLNLNCTKVTASQGH
                     GYGNFVLSGNCDEAIIKVISNGFGDTRSLHVANKLVVVSDTPVNTSVNAQGAASTFEI
                     NGTGDIIYYGTPSTLSVIQYGKGNVVKAE"
     gene            complement(2717..4309)
                     /locus_tag="PKN22_01105"
     CDS             complement(2717..4309)
                     /locus_tag="PKN22_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNS41329.1"
                     /translation="MEKRNNKFWVPILIGLALAGGIFVGRKMALPNDLIFGIQSNKSA
                     SNQKIQDIIDIIDNYYVDSVNSEELLEKTISDMLHQLDPHSNYISAKDMLLAKEQIKG
                     EFSGVGIRFLSLRDTICVINVVKGSPSDAVGVKAGDKILKVDGKTVAGVKIKTDDIMS
                     RLKGKANTKVNVVFLRDGKTLNKTITRGTIPIYSVSAAFMMNKETGFVKIDQFSITTA
                     SEFRNAAKQLLASGMKSMILDLRNNSGGVMQSATTIVDEFLPANKVIVSTKGTHLKEQ
                     FYKSSRDGILEDVKVVVLINENSASASEIVAGALQDNDRATIVGRRSFGKGLVQEDMV
                     LRDNSSLRLTVARYYTPTGRSIQKPYNGDIEEYYHEEMSRYDKGELFQPDSSLFVDSL
                     KYTTPKGKIVYGGGGIMPDVFVPLDTTGNSWYLTQLRYAMTFQSFAFDFAHGKYEKWK
                     NPQEYAATFNVSDNLMQQFLNYAQKEFKIAIDQKGLRMSKSLLKLFLKAEIARQIWVE
                     DGYYTVVSQDDKEVKAALQQLK"
     gene            complement(4309..4740)
                     /locus_tag="PKN22_01110"
     CDS             complement(4309..4740)
                     /locus_tag="PKN22_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNS41330.1"
                     /translation="MSKKQDKQLKYDLAYLKMAKSWSELSHCSRKKVGALIVKDDMII
                     SDGYNGTPSGFDNCCEDDNGDTLWYVLHAEANAILKVAKSTNNSKGATLYLTLSPCKD
                     CSKLVLQAGITRVVFSDNYKDTSGVDFLKNAGIEVVQLQLD"
     gene            complement(4859..7639)
                     /gene="acnA"
                     /locus_tag="PKN22_01115"
     CDS             complement(4859..7639)
                     /gene="acnA"
                     /locus_tag="PKN22_01115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNS41331.1"
                     /translation="MSYSKAKSVRELSVNGKNYKFSSLKDLKPGGIEHLPFSIRILLE
                     NVLRNYDGFSITDEHIETLLNWTPEENDKDIPFKPGRVLMQDFTGVPAVVDMASLRAE
                     FVRHGKDGQKINPAIRVDLVIDHSVQVDYFGTDYSIGKNVDLEYERNKERYELLKWAQ
                     KGLNNFTVVPPGMGICHQVNLEYLAKGVTERNGWLFPDTLVGTDSHTPMVNGIGVVAW
                     GVGGIEAEAAMLDQPTFFTCPQVVGLKLTGSIPNHCTATDMVLTITRLLRDTGVVGKF
                     VEVFGDGLDNLTVTDRATIANMSPEFGCTVTYFPIDNRTLEYMHSTNRSAEQIKIVET
                     YCKENLLWRTGNEKISYSKVVELDLSTLEPTVSGPKRPQDKIFVKDLGSSFKTLLKSE
                     FNRDYLVKDERQESAWLKEGGSGTEFTFGKVPLNGESHSEVALDENKLRSVRIKQANK
                     EFVLSDGNIVIAAITSCTNTSNPAVMVGAGLLARNAVEKGLRTKSWVKTSLAPGSKVV
                     TKYLERSGLNVDLEALRFHTVGYGCTSCIGNSGPLPPAIATAVDKGELVVASVLSGNR
                     NFEARVHPQVKMNFLMSPMLVVAYALVGRVDIDLINDPLDYDPNGQPVYLKDIWPTRD
                     EIRKTINECVKQEDFGEIYDVIFDGSTDWQTLEVKLSESYEWNQQSTYIKEAPFFENI
                     TAETKPITDINGARVLLYLGDSVTTDHISPAGSFKEGTAAGQYLLSNGVAKEDFNSYG
                     SRRGNHEVMMRGTFANVRIKNKVADKEGGFSRYFPTNEVNTVFDVAMQYKKDNTPLVV
                     LAGKEYGSGSSRDWAAKGTFLLGVKAVIAESYERIHRSNLVGMGVAPLVFTNGENAES
                     LGLDGSETFDITGLADNLVPRKILDVKATHPSGKVTNFKVEARLDSQIEIEYYKNEGI
                     LQYVLRDYLKKN"
     gene            7896..8906
                     /locus_tag="PKN22_01120"
     CDS             7896..8906
                     /locus_tag="PKN22_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS41332.1"
                     /translation="MAQICGWSIYSLLIYFSATIDRPTEISSNLHLQLVFMTVFNIFF
                     THIMRLYMLRNNWLDKKLAQLLPRLIIISIICSAFIECCLLVSEYFLSDNFYNDLKPA
                     QVIINILALTLLIISWNGIYFTYHFFQKSRVKELENVTLEASKNEIELKNLRSQLNPH
                     FLFNSLNSIRALVDIEPQNAKNAITTLSNLLRKSLVSGKENLIPVSEEVEIVKSYLDL
                     EKIRFEERLTIDWQLDERCNSFLLPPFIIQTLAENAIKHGISQQINGGFIRISIQNKN
                     GKLLITVENTGQLNNSVDSGIGIANTERRLAIQFKDKAAFSLQQLETTVVATLTFDYE
                     EN"
     gene            8893..9639
                     /locus_tag="PKN22_01125"
     CDS             8893..9639
                     /locus_tag="PKN22_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS41333.1"
                     /translation="MKKIKTLLIDDERLAREELKSLLKNYSQIEIIDEAKNGQEGIQL
                     INELHPDLIFLDVNIPGITGFDLLKQLDEIPLVVFVTAYDEYALKAFEVDALDYLLKP
                     VDPNRLDETIKKITQRFEEENATIAPSETGFEKKQLSINDRVFLKDGEKCWFVQLSDI
                     RFFESDGNYVKVHFNGFKPMILSSLGNLEERLDTTYFFRANRKYIINIQWITKIENWF
                     NGGLMVELESGDKVEISRRQAIRFKELWAL"
     gene            complement(9684..10868)
                     /locus_tag="PKN22_01130"
     CDS             complement(9684..10868)
                     /locus_tag="PKN22_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41334.1"
                     /translation="MKKIVIGLFLVVGFQLQAQMKSGFEPTEARDMIQLCNSFGYLDL
                     YGSDTEILPVGYEKVYTSPSLGMDNKFQVYKKGNKGAIVFRGTTSNMLSFMENFYAAM
                     LPVKGTIKIKGEKFKYQMGENANSSIHAGYTLAIGYMKSELLHQINELNRQGVYDIFI
                     TGHSQGGALATLVRAYFNYVPDKELSKKNTFKVYAFANPMVGNATFAKEYDANFCTPQ
                     MSYSIHNPKDFVTKLPVSKADAAFWENNTELLAGGNERMGALSAFLGGAGTMFKGRIV
                     EMAKTMAIQIEKMLQNSLGNFVMPQFKDEFDYVHESNIVEISTTEYPLELKDSSVLQN
                     DSILKTRDANGIFPNKDLYKPTNSFLQHKPYNYYTALLKDYFPEEYQALKQKYFVMPE
                     KK"
     gene            complement(10949..>12576)
                     /locus_tag="PKN22_01135"
     CDS             complement(10949..>12576)
                     /locus_tag="PKN22_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS41335.1"
                     /translation="ALFVVPFLSSVILKPHKNEGGNIILKGLQKIIHLTYAVWLDKAL
                     KKPVLTVIGAALIFAGSLFMLPRLGFELFPSSERPQFMVHIAPPPQSNIYYSDSITRI
                     IEADLSKMKKVKYYSSNVGKGNPRLFYNLEQASEKQDFAEIFVQLQNNVSSDDKLKII
                     DSLKKHWSSFLGAKIEVRNFEQGVPILSPVEVRLFGDNLDTLKQLASRVEVMLKKTKG
                     TEDVNNPNNFSKMDLKVNINREKAAALGVPSALIDRSIRMALSGLDVATFSDPENDNT
                     DYKIRVTVPRGDYPDMSIFDQIYVDNVQGMSIPIKQLIELNMESSPSSISHIDRTRVV
                     YVNSFVKKGYTNDKVIADVENQMKKMKLPYGYHFKMGGEVESKEEAFGGFGTIIIITL
                     FLFIAVLVMEFKTLKSTLIVLSVIPLGIVGAVIALWITGNPLSFVSTVGLVALAGIEV
                     KNTILLVDFTNQLRKEGMDIIEAAEKASEIRFLPIILTTLTAIGGLIPIALSSNPMIA
                     PLAIVIIGGLISSTLLSRIVTPVVYRLIPPKID"
BASE COUNT         3765 a         2581 c         2135 g         4095 t
ORIGIN      
        1 atgcgcaata aaaacaacta atttatttca atattttttt ggcttgttcc caataaacat
       61 ccaactcttc aagcgataat tcgcccattt gctgttggtt gttgtgcaac aattcttcca
      121 ttttcatgaa tcgagcaatg aattttttgt tggttttttc caatgcattt tcaggattta
      181 caccaataaa acgcgagtaa ttgattaaag agaataacaa atcaccaaat tcatcttcaa
      241 tttgcggcga ttgtgcgtcc acttctgcct taaattcgtt caattcttct tgaactttat
      301 tccaaacttg ttctttattg tcccaatcaa aaccaacgcc ttttactttt tcttgaatac
      361 gcgcggcttt taccattgct ggcaacgatt gcggtacgcc ttgcaaaaca gatttttttc
      421 cttctttaag tttcagtttt tcccaattgg cttttacttg ttcttcggtt tcggcttgta
      481 catcaccata aatatgcgga tgacggtgta ccaatttatc gcaaatgcgg tttaaaaccg
      541 aactcacatc aaaagcaccc acttcacttc ccaacttgct gtaaaaaacc atgtgcagaa
      601 aaatatcacc aatttcgcct tccaattctt tcaaatcgtt gtccaaaatc gcatccgcca
      661 actcataggt ttcttctatt gtcaagtgtc tcagactttc aaaggtttgt tttttatccc
      721 acggacattt ttcgcgcagg tcgtccatga tgtccaataa tctgttaaaa gcctttaatc
      781 gttcatccat tttatttact tttgccacaa atttagtaaa tttcgcaacc attactattt
      841 aaaaacaaag tatgcgtagt gttcttatct gttgtttact ggcattaagc agcgtttctt
      901 ttgcccaaga aaaaataaat tggggattag aagctcgaat gaaatatggg tttcttattg
      961 gtcaccgcgt ggtaatggga cacattgcgc aaaatcacac ttatgcagca gaattggcgt
     1021 ttgtgtaccg accaaatggt gaaaaagcat ggcataaata ctataaatat cccgatttat
     1081 ccatcaattt gtattttgga aacgttggaa acaccaagtt attggggaat tttggtggaa
     1141 tttatggatc attggccatt ccatttgttg cccgcaaaca ttttcgattg agtggaaaat
     1201 taggttgtgg attgggctat acgaataaag tatatgatga agttctgaac aacaaaaatt
     1261 cggctatttc tactcgcatc aacgcgttga ttaatttagg aatagatacc cgaacatatt
     1321 ttaaaaacaa ctggatattg tttggaatag atttgacaca tttctccaat ggcggaacaa
     1381 aagtgcccaa tttaggaatc aacattccgc aaattacact gggatacgga cgttttattc
     1441 actacaacga aaaacattct accatagaaa aaccaactta tattccacaa cgacgtttgt
     1501 tgtttggagc cacagccata gcctctgcca agcaagtttt tccaaccaac ggaagaactt
     1561 atcctgtttt tgcattgaac gtgcacatgc gtatgtttat gaaaccgcgt gttggttggg
     1621 aggttgcctt tgatgcgatg tccaatcaat ccatttttgg ataccgtcca gaggtgcaaa
     1681 aaacacaatg gaaagtgatg cagtttggtg tttttgcagg atatttattg ccgctcaata
     1741 atttccattt tgtattggga atgggtgcct atgtgcgtga tttttacaaa cccgaagaac
     1801 ttttttatca ccgtgtggga atgcgttatt acctaaaaag tggtgtgcat ttcaacttgg
     1861 ttttaaaatc gcattggggc aaagccgatt atgtggaatg gggcgttggc tatacatttt
     1921 ttaaccgcaa aaaagacaaa aaatgagatt cgttgcacta attttaatcg taagcgcttt
     1981 tactgcttgt aaaaaagcag aagatcgtcg ttgcatgaaa gctgttggcg acaatgtaac
     2041 attagaacta gcattgcccg agtttgacaa attgcgtgtt ggacctaaaa tggaagttgt
     2101 tttggtgcaa gatacagtca ataaaatgat tgtcaacggt ggagaaaatt taattaattt
     2161 catcactgct gagatgaaca acgaaggtta tttgttgctc aaaaactcca ataaatgtga
     2221 tttcttacgc tcgtataaaa agagtttgat taaagttgaa cttcatttta aagaaattcg
     2281 caacttattt tttgaaggta cgcacgacat tacaacactc ggagccatta ccaccaacac
     2341 atttgggttg gaaattcaag acagtggcgc aaccgcctat ttgaatttga attgtacaaa
     2401 agttaccgct tcgcaaggtc acggctatgg taattttgta ttgagtggaa attgcgacga
     2461 agccatcatc aaagtcatta gcaacggttt tggcgataca agaagtttgc atgttgccaa
     2521 caaattagta gttgtttccg acacacctgt caacacatcc gtaaatgcgc aaggagctgc
     2581 ctctacgttt gaaatcaacg gaacgggcga cattatttat tacggcaccc cttctactct
     2641 ttccgtaatt cagtatggaa aaggaaatgt ggtgaaagcg gagtgagttg cactatcccc
     2701 ccctacttct ccatcatcac ttcaattgtt gcaaggctgc ttttacttct ttgtcgtctt
     2761 ggctaacaac ggtgtaatat ccgtcttcta cccagatttg gcgagcaatt tctgctttta
     2821 aaaacaattt taacaacgac ttgctcatac gcaatccttt ttggtcaata gcaattttaa
     2881 attctttttg agcgtaattt aaaaactgtt gcatcaaatt gtctgaaaca ttgaaagtgg
     2941 ctgcatattc ttgcggattt ttccattttt cgtatttacc atgtgcaaaa tcaaatgcaa
     3001 acgattgaaa agtcatggca tatcgcaact gggttaaata ccaactattg cctgtggtat
     3061 ccagcggaac aaatacatcc ggcatgattc ctcctccacc gtaaacaatt ttgccttttg
     3121 gcgttgtgta tttcaatgaa tctacaaaca acgatgaatc gggttgaaac aattcacctt
     3181 tgtcatagcg actcatttct tcgtggtagt attcttcaat atcaccgttg tatggttttt
     3241 gaatgcttcg tcctgttggt gtgtaatagc gcgcaactgt taaacgcaaa ctgctgttgt
     3301 ctctcaacac catgtcttct tgcaccaaac ctttaccaaa actacggcgt ccaacaatgg
     3361 ttgcacggtc gttatcttgc aatgcacctg ccacaatttc acttgcagaa gctgagtttt
     3421 cgttgatcaa cacgaccact ttcacatctt ccaaaatgcc atcgcgcgaa gatttgtaaa
     3481 attgctcttt caaatgcgtt ccttttgtag aaacaatcac tttattggca ggcaaaaatt
     3541 catccacaat ggttgttgca ctttgcataa cacctcctga attattgcgt aaatccaaaa
     3601 tcatgctttt cataccgctt gccaacaatt gttttgcagc atttctaaat tcgctagccg
     3661 tggtgattga aaactgatcg attttcacaa atccagtttc tttgttcatc ataaatgccg
     3721 ccgaaacaga ataaattgga atggttcctc gtgtgatggt tttgttcaat gtttttccat
     3781 cgcgtaaaaa cacaacgttg actttggtat tggcttttcc tttgaggcgc gacatgatat
     3841 cgtcggtttt aattttaaca ccagccaccg ttttgccatc tactttcaaa attttatcgc
     3901 ctgcctttac acccactgca tcagagggag aacctttcac cacattgata acacaaatgg
     3961 tatcgcgaag ggataaaaaa cgaattccaa cacccgaaaa ctcgcctttt atttgctctt
     4021 ttgccaacaa catgtctttt gccgaaatgt agttggaatg aggatcgagc tggtgcaaca
     4081 tatcactgat ggttttctcc aacaattctt cgctgttgac actgtccaca taataattat
     4141 cgatgatgtc gatgatatct tgaatttttt ggttggaagc agatttattg gattgtattc
     4201 caaaaatcaa atcgttgggc aaagccattt ttcgtccaac aaaaatacct cctgctagcg
     4261 ccaaaccaat gaggatgggc acccaaaatt tattgtttct tttttccatc aatctaattg
     4321 taattgaacc acttcaatgc ccgcattttt taaaaaatct acgcccgaag tatctttgta
     4381 attatctgaa aacacaacgc gtgtaattcc agcttgcaaa accaacttac tacaatcttt
     4441 gcacggcgac aaggttaaat acaaagttgc acctttgcta ttgttggttg attttgccac
     4501 ttttaaaatt gcgttggctt cggcatgcaa gacataccac aatgtatctc cattatcgtc
     4561 ttcgcaacaa ttatcaaaac cagatggtgt tccgttgtat ccatccgaaa taatcatgtc
     4621 gtcttttacg attaatgctc caactttttt gcggctgcaa tgtgacaatt ccgaccaact
     4681 ttttgccatt ttcaaataag ccaaatcgta ttttaattgt ttgtcttgtt ttttgctcat
     4741 agttgacaaa aataaacaat tcctattaga aagagtaatg cattgccaac tttcgttcat
     4801 gaaataaaaa aagagccgtt cccagtagaa acagctctta tttttatgcg attattgctt
     4861 aatttttctt caaataatct cttaaaacgt attgcaaaat accttcgttt ttatagtatt
     4921 caatttcaat ttgagagtcc aaacgtgctt ctactttaaa attggttact tttccagaag
     4981 gatgtgttgc ttttacatct aaaattttgc gaggcacaag gttgtcagcc aatcctgtga
     5041 tatcaaacgt ttcagagcca tccaatccaa gcgattcggc attttctcca tttgtaaata
     5101 ccaacggtgc aacgcccatt cccactaaat tgctgcggtg aatacgttca taactttcgg
     5161 caattaccgc ttttacccct aataaaaacg ttccttttgc tgcccagtca cgtgatgagc
     5221 ccgaaccata ttccttacct gccaaaacta ccaacggtgt attgtctttt ttgtattgca
     5281 tggcaacatc aaacacggtg ttaacttcgt tggttgggaa ataacggcta aatcctcctt
     5341 ctttatctgc cactttattt ttgatgcgta cattggcaaa cgttccacgc atcatcactt
     5401 cgtggtttcc tctacgtgaa ccatacgagt tgaaatcttc ttttgccaca ccattgctca
     5461 acaaatactg accagcagct gttccttctt taaacgatcc tgcaggcgaa atgtggtcgg
     5521 ttgtaacaga atctcctaaa tacaacaaca ctctagctcc gtttatatcg gtaattggtt
     5581 ttgtttcggc agtgatgttt tcgaaaaatg gcgcctcttt gatgtaggtt gattgttgat
     5641 tccattcata gctttcgctc aacttcactt ccaatgtttg ccaatcggtt gaaccatcaa
     5701 aaatcacatc gtaaatttca ccaaaatctt cttgttttac acactcattg atggtctttc
     5761 taatctcatc gcgagttggc caaatatctt tcaaataaac aggttgtcca tttggatcgt
     5821 aatccaacgg atcattgatt aggtcgatat ctactctacc caccaatgca tacgccacca
     5881 ccaacatagg cgacatcaag aagttcattt ttacttgcgg atgcacacgt gcttcaaagt
     5941 ttctgtttcc agaaagaaca gatgcaacca ccaattcgcc tttatcaaca gctgttgcaa
     6001 ttgctggagg aagtggtcct gagttaccaa tacatgatgt acaaccataa cctaccgtgt
     6061 ggaaacgcaa tgcttctaaa tcaacgttta aacctgaacg ttctaagtat ttggtaacaa
     6121 ctttagaacc tggtgccaac gatgttttta cccacgattt ggtgcgcaac cctttttcaa
     6181 ctgcatttct agccaacaat ccagctccca ccattaccgc agggttggac gtattggtac
     6241 aactggtaat tgctgcaata acaatatttc catcactcaa aacaaactct ttgttggctt
     6301 gtttgatgcg aacagaacgc aatttatttt cgtccaaagc aacttcagag tgcgattcac
     6361 catttaatgg aacttttcca aaagtaaatt ctgtgcccga accaccttct tttaaccaag
     6421 cagattcttg acgttcgtct tttaccaaat aatcgcggtt aaactcgctt ttcaacaaag
     6481 ttttgaacga agaaccaagg tcttttacaa atattttatc ttgtggacgt tttggaccag
     6541 aaactgttgg ttcaagtgtc gataaatcca attcaactac ttttgaatac gagatttttt
     6601 cgttgcctgt gcgccacaat aagttttctt tacaataggt ttcaacaatt tttatttgct
     6661 ctgccgaacg gttagttgaa tgcatatact ccaacgtgcg attatcaatt gggaaataag
     6721 tcaccgtgca accaaattca ggcgacatgt tggcaatcgt tgcgcggtcg gttactgtta
     6781 aattgtccaa tccatctcca aaaacttcta caaatttacc cacaacacca gtgtcgcgca
     6841 acaaacgagt gatggtcaac accatatcgg ttgccgtaca atgattaggg atgcttcctg
     6901 ttaatttcaa tcctacaact tgcggacaag taaaaaatgt tggttggtct aacatcgcag
     6961 cttcggcttc aattccacca actccccatg caacaactcc aattccgttt accattggcg
     7021 tgtgtgaatc tgttccaacc aaggtatctg ggaacaacca accatttctt tcggtaacac
     7081 cttttgccaa atattctaag tttacttggt gacaaatccc cattcctgga ggcaccaccg
     7141 taaaattatt caatcctttt tgtgcccatt tcaacaattc atagcgttct ttgttgcgtt
     7201 cgtattccaa atccacgttt ttaccgatgg aataatctgt tccgaaataa tcgacttgca
     7261 ccgagtggtc aattaccaaa tcaacccgaa ttgcaggatt gattttttgt ccatcttttc
     7321 cgtgacgtac aaattctgca cgcaacgaag ccatatccac aacggctgga acacccgtaa
     7381 aatcttgcat caaaacacgc ccaggtttaa aaggaatgtc tttatcgttt tcttctggtg
     7441 tccaatttaa tagggtctca atatgttcat ccgttatgct aaatccatcg taattcctca
     7501 gtacgttttc caacaaaatg cggatactaa acggtaaatg ctcaattcct ccaggtttta
     7561 aatcttttag tgaactaaat ttgtaatttt taccattgac tgacagctct cttactgact
     7621 ttgcttttga ataactcata agtgatctat tttgtttgta tataaaagta atgttgcaag
     7681 gttgttttaa aaatattgag gtagaaacaa ccctactttc caaatttaac ctttttggat
     7741 gccaataaaa aattagaacg attaaaattt gttaattaaa atcattcttg ataatatttt
     7801 agtcgtttta ttctcaacaa gttaataaaa cactgcgata gattcaaaat cattgttatt
     7861 tttgtttcat ggagaacagc aaaaaaatgt attgggtggc gcaaatctgt ggatggagta
     7921 tttattcgct gctcatttat ttttctgcta cgatagatcg cccaacagaa atttcatcca
     7981 acctccattt acaattggtt ttcatgactg tgttcaacat tttctttacg cacattatgc
     8041 gcttgtatat gttgcgcaac aactggttgg ataaaaaatt agcgcaatta cttcctcgtt
     8101 taatcatcat ttcaattatt tgttctgcct ttattgaatg ttgcttattg gtttcagaat
     8161 attttttatc ggataatttt tacaacgatt taaaaccagc tcaagtaatc attaatattt
     8221 tggcgcttac tttattgatt atcagttgga atggaattta ttttacctat catttttttc
     8281 aaaaatcgcg tgtaaaagaa ttggaaaacg tgactttaga agcgtcaaaa aatgaaattg
     8341 aattaaagaa tttacgttcg caattgaacc ctcatttttt attcaactca ctcaattcta
     8401 tccgcgcttt ggtggatatt gaacctcaaa atgctaaaaa tgccatcaca acgctttcca
     8461 atttattgcg taaatcgttg gtttcgggca aagaaaattt gattcctgtt agcgaagaag
     8521 tagaaattgt aaaaagctac ctcgatcttg aaaaaataag gtttgaagaa cgattgacaa
     8581 tcgactggca attggatgaa cgttgcaatt cgtttttact tcctccgttt atcattcaaa
     8641 cattagctga aaacgccata aaacatggaa tttcacaaca aataaatggt ggatttatac
     8701 gcatttctat tcaaaataag aacggaaaac tgctaattac cgttgaaaat acaggacaac
     8761 tcaacaactc cgttgatagc ggtattggta ttgcaaacac cgagcgacgt ttggctatcc
     8821 aatttaaaga caaagcagca ttttcattgc aacaattaga aacaaccgtt gtggcaacat
     8881 taacttttga ttatgaagaa aattaaaaca ttactcattg atgatgagcg acttgcgcgt
     8941 gaagaattaa agtcattatt gaaaaactat tcgcaaatag aaatcattga cgaagcaaaa
     9001 aacggtcaag aaggtatcca attaatcaac gaacttcatc ctgatttaat tttcctcgat
     9061 gtcaacatac ctggaattac tgggtttgat ttattgaaac agctggatga aattccactc
     9121 gttgtatttg ttactgccta cgatgaatat gctttgaaag cttttgaagt ggatgccttg
     9181 gattatttgc taaaacctgt tgaccccaac cgattggatg aaacgattaa gaaaatcacc
     9241 caacgatttg aggaagaaaa tgccaccatc gctccttctg aaacagggtt tgaaaagaaa
     9301 caattgtcca tcaacgaccg tgtgttttta aaagacggcg aaaaatgttg gtttgttcaa
     9361 ctttccgata tccgcttttt tgaatcggat ggtaattatg taaaggttca tttcaacggt
     9421 tttaaaccaa tgattttaag ttctttaggt aacttagaag aacgtttaga cacaacttat
     9481 ttcttccgtg ccaatagaaa atacatcatt aacattcaat ggattaccaa aattgaaaac
     9541 tggtttaacg gcggcttaat ggtggaatta gaatcggggg ataaagttga aatttctcgt
     9601 cgacaagcca ttcgtttcaa agagctgtgg gcactataaa gcattcaatt tttagtaaaa
     9661 cagagattaa atcaaacttc tatctacttc ttctctggca tcacaaaata cttttgtttc
     9721 aaagcttggt attcttctgg aaaataatct tttagcaatg ctgtgtaata attatacggc
     9781 ttgtgttgca aaaaactatt ggttggttta tacaaatctt tgttaggaaa aatgccgttt
     9841 gcatctcttg tttttaggat ggaatcgttt tgtaaaacag acgaatcttt caattccaac
     9901 ggatattcgg ttgttgaaat ttctacaata ttggattcat gcacataatc aaactcatct
     9961 ttaaactgag gcattacaaa gtttcccaaa ctattttgaa gcatcttttc gatttgaata
    10021 gccatcgttt ttgccatttc aacaattctt cctttaaaca tggttcctgc tccacccaaa
    10081 aaagcggata gtgcccccat gcgttcattt ccgcctgcca ataattcggt gttattttcc
    10141 caaaatgcag catccgcctt gcttacaggc aattttgtaa caaaatcttt agggttatga
    10201 atggaataac tcatctgtgg tgtacaaaaa ttagcgtcgt attctttggc aaaagtggca
    10261 tttccaacca ttggattggc aaaagcatac actttaaacg tgttcttttt gctcaattct
    10321 ttatctggaa cataattgaa ataggcacgc accaacgtag ccaaagcacc tccttgcgaa
    10381 tgtccggtta taaaaatatc gtaaacacct tgtctgttca actcgtttat ttggtgcaat
    10441 aattcacttt tcatataccc aattgcgagc gtataaccag cgtgaatgga actattggcg
    10501 ttttcgccca tttggtattt gaatttttct ccttttattt taatggtgcc tttaactgga
    10561 agcattgctg cataaaagtt ttccataaaa cttagcatat ttgaagtagt accacgaaaa
    10621 actatcgctc ctttgttacc ctttttatac acctgaaatt tattgtccat tcccaacgat
    10681 ggcgatgtgt acactttttc atatccaaca ggcaaaattt ctgtatcact gccatataaa
    10741 tccaaatagc caaaactgtt gcacaactga atcatatcgc gtgcttcggt aggttcaaaa
    10801 ccacttttca tttgtgcttg aagttggaaa ccaacaacaa gaaatagtcc aattacaatc
    10861 tttttcatcc tatttatttt aaatgtataa ctctttttaa atcattttat tactcgttag
    10921 agtggtttac aattcaaaaa gttacaattt aatcaatttt tggtggaatt aatcggtaaa
    10981 caacaggtgt aacaatgcgc gacaacaacg ttgaacttat caaacctcca ataatcacaa
    11041 tggcaagtgg cgcaatcatt gggttactcg acaatgcaat tggaattaaa ccaccaatgg
    11101 ctgttaaagt tgttagaata atcggcaaga aacgtatttc ggatgctttt tcagcagctt
    11161 cgatgatgtc catgccttct ttgcgcaatt gattggtaaa atcgaccaat aaaatggtgt
    11221 ttttcacttc aattccagcc aaagctacca atccaacagt tgaaacaaac gacaacggat
    11281 ttccagttat ccaaagcgca attacagccc ccacaatacc gagcggaatt acagacaata
    11341 caatcagcgt actttttagc gttttaaatt ccattaccaa cacagcaatg aataaaaaca
    11401 acgttatgat gatgatggtt ccaaatccgc caaaagcttc ttccttactt tcaacttctc
    11461 cccccatttt aaaatgataa ccataaggca atttcatttt tttcatctga ttttcaacat
    11521 ctgcaattac tttgtcgttg gtatatcctt ttttcacaaa cgagttgaca tataccactc
    11581 gtgttctgtc gatgtgacta atagacgacg gcgacgattc catgtttaat tctatcaatt
    11641 gcttgatggg aattgacatt ccttgcacat tatccacata aatttgatca aaaattgaca
    11701 tatccggata atcgccacga ggaaccgtta cacgaatttt atagtctgta ttatcgtttt
    11761 ctggatcgga aaaagtagca acatccaaac cggataatgc catacgaatg gaacggtcaa
    11821 tcaacgccga aggaactccc aaagcggctg ctttttcacg attgatgttc acttttaaat
    11881 ccatttttga aaagttattt ggattattga catcttcggt tcctttcgtt ttcttcaaca
    11941 ttacttctac tctgcttgcc aattgtttga gtgtatccaa attatcgcca aacaaacgca
    12001 cctctactgg tgaaagtatt ggaacgcctt gttcaaagtt tctaacctca atttttgctc
    12061 ctaaaaatga actccaatgt tttttcaacg aatcaatgat ttttaattta tcatcagagc
    12121 ttacgttgtt ttgtaattgt acaaaaattt ctgcaaaatc ttgtttttca cttgcttgtt
    12181 ctaagttgta aaacaaacgc ggatttcctt ttccaacatt gctggaatag tattttacct
    12241 ttttcatttt gctcaaatca gcctcaatga tgcgtgtaat actgtctgaa taataaatat
    12301 tggattgagg tggtggcgca atgtgcacca taaattgtgg tcgttctgag gatggaaaca
    12361 actcaaaccc caagcgaggc aacatgaaca acgaacccgc aaaaataaga gcagcaccaa
    12421 tcaccgttaa aactggtttt ttcaatgctt tatccaacca aacagcatag gttaaatgaa
    12481 tgattttttg caatcctttt aaaatgatat ttcccccctc atttttatgc ggttttaaaa
    12541 taacactgga taaaaacggc acaacaaaca gggcaa
//
LOCUS       DAOQVQ010000017        12402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5774_length_12402_cov_3.890581, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000017 DAOQVQ010000000
VERSION     DAOQVQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5774_length_12402_cov_3.890581"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..475)
                     /locus_tag="PKN22_01140"
     CDS             complement(131..475)
                     /locus_tag="PKN22_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41336.1"
                     /translation="MNISNNYWTNDQTRKEVITKTLIPNVRNFDLYATYYNLMDKRGL
                     SFPLNFENDLVVEKLYRKNIFEYIILEHNGQKELYILPNETLVEFSDRVASKIDNCTS
                     EAEVLDLLFAEM"
     gene            complement(479..1255)
                     /locus_tag="PKN22_01145"
     CDS             complement(479..1255)
                     /locus_tag="PKN22_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNS41337.1"
                     /translation="MERKLWTKDELILVFNLYLKLPFGKMHTRTPEIIEMANLLGRTV
                     NSIAIRLTNFAACDPYHQSRGVKGMVGGIKQCQPIWDEFAKNKELLIFESEQILAKKE
                     NQTIETKFEDLLFDLKDLKGETKIREVKTRVNQNVFRQIVIANYSGKCAITGIDIPEL
                     LFASHIIPWSKNEDERLNPENGICLSALYDKAYDKGLIAINEKYKILISDKLKNKKDT
                     DYYGKYFAPIEYQSLIQPQRYFPKKDFIQYHLDVIFNKNF"
     gene            complement(1470..2729)
                     /gene="odhB"
                     /locus_tag="PKN22_01150"
     CDS             complement(1470..2729)
                     /gene="odhB"
                     /locus_tag="PKN22_01150"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNS41338.1"
                     /translation="MSVLEMKVPSPGESISEVEIAQWLVSDGDYVEKDQAIAEVDSDK
                     ATLELPAEASGTITLKAGEGDVVKVGQVVCLIDTSAARPEGAATPVANKETVATPAPE
                     AKKEEAKAVVPSPEPAKNYATGVSSPAAAKIANENGVNVAKISGTGKNGRITKQDVLA
                     AMSGGFATDAVQGWGGTREQRREKMSMLRRKIAERLVSVKNDTAMLTTFNEVDMKPIM
                     DLRAKYKDRFAKHHEVNLGFMSFFTKAVTEALNLYPAVNSMIDGNDMIFHDYADIGIA
                     VSSPKGLMVPVVRNAEQMSLAEIEAEIKRLAIKARDGKITPDDMMGGTFTITNGGVFG
                     SMMSTPIINPPQSAILGMHNIVERPVAIDGKVEIRPMMYLALSYDHRVIDGRESVGFL
                     VKVKEMLENPERMLFGGKDPYEVLLGL"
     gene            complement(2769..5483)
                     /locus_tag="PKN22_01155"
     CDS             complement(2769..5483)
                     /locus_tag="PKN22_01155"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNS41339.1"
                     /translation="MDNISYVGNADVNAIDHLYKMYRQDPESVDIGWKRFFEGFEFSK
                     ANYDEDVPENFQKELKVLNLINGYRTRGHLFTKTNPVRERRQYEPTLELSNFGLEDSD
                     LDTVFKAGEEVGLGATTLRAIVDHLQKTYCQSIGIEFMYMNNPQKVKWMLNAIELANR
                     PTFTNEQKVEFYSQLMQASEFEGFLAKKFVGQKRFSIEGVEALIPALKTIINKGAEKG
                     VESFIMGMAHRGRLNTLTNIFEKRPKDIFNEFKGKEFDAEDFDGDVKYHLGYTTKVNK
                     SGKEVSLTLCPNPSHLEAVGPVVQGLSRATLDAKYSNDLNKVCPVIIHGDAAVAGQGV
                     VYEVIQMAALKGYKTGGTIHIITNNQVGFTTNFTDSRSSIYCTDIAKTTLAPVFHVNA
                     DDVEAVIQAVEIAVAYRQEFKQDIFIDLLGYRKYGHNEGDEPKFTQPNLYKAIAKHKN
                     PSELYIETLIPQGVLSREDAKKKIDAYQNFLEIEFDASAKQEKATVFNFMEEEWKGMK
                     TATAADFEQSVDTSIPKKKLLELGKKIATLPEGKTYFRKIQKIFSDRLEAITTDQLDW
                     GSAEMLAYATLLEEGHPVRITGQDVERGTFSHRHAVVKTEDTEEEIVTLNLLGDKQAR
                     FSIYNSLLSEYAVLGFEYGYSLASPKGLTIWEAQFGDFYNGAQIMVDQFITAGEDKWS
                     VQSGLVMLLPHGYEGQGAEHSSGRMERFLQQAADLNIQVANTSTPANHFHLLRRQLSR
                     SFRKPLVVFSPKMLLRYPTAVSSLDDMAKGGFQEVIDDPQAKAANIETVVLCTGKFYY
                     EATEKAAELGVDNMAFVRVEQLYPFPEKQIEAVLAKYKNKKRIVWAQEEPENMGAWQY
                     MAMRMRGKDLIGVTRPAAAAAAEGSKELHLKRLNALWNNLFQYAAVKA"
     gene            complement(5671..6945)
                     /locus_tag="PKN22_01160"
     CDS             complement(5671..6945)
                     /locus_tag="PKN22_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41340.1"
                     /translation="MKTLLSILLLLPIFAQAQARLVFNNGAYINIENQAYLVVDNGAA
                     NAITLLGSGGNILSENENDVVKWNIGNNTDVHHVPFTNTNGIKVPAIVDVTTAGSATG
                     NLIFSTYRTANNNTPYPSDVNNMCSSVGPMDASLYAVDRFWRIDPAAYATRPAVSLSF
                     GYDFVNEAAPANTITESNLQAQRFNPGAGAGNYCVGAPSVGSWQDLLFGTVNTATKQV
                     NNAVISPANFFKSWVLVDVSHPLPITLAGFNANCEQKIQLNWSTASEQNSDKFVVEKR
                     NNDTDWEYVGEVNAAGNSHQLLNYYYEDNRSWASTTYYRLRLINFDGQQQIYGPISVS
                     CEASENSLQVYPSPNSGSFVVAIHSSSEIQNGAVQVYDLAGKVVYSVNVNALSGTTLV
                     PITHLEVVPGAYFVKLLGGDEALQTPVKVIID"
     gene            complement(6942..7322)
                     /locus_tag="PKN22_01165"
     CDS             complement(6942..7322)
                     /locus_tag="PKN22_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41341.1"
                     /translation="MKTSCFLFAFFMVVNLTNAQQNKEKSKPIQVNLALTSQSNNVSL
                     TLTKSNYTHKQINNKSATPIPSKLSFHSNSNANVFIVDTNSNNRLKLVNNHKEIVPKT
                     NISKLSLTPVKADVKFIINEKNKQ"
     gene            complement(7328..7543)
                     /locus_tag="PKN22_01170"
     CDS             complement(7328..7543)
                     /locus_tag="PKN22_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41342.1"
                     /translation="MTFTITDPDALCGTYSSAISINFDCNLNPLLYQGITIRNVVVDA
                     GQIIVNVLNDSPYDITGSIPITVLAIH"
     gene            complement(7637..8218)
                     /locus_tag="PKN22_01175"
     CDS             complement(7637..8218)
                     /locus_tag="PKN22_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41343.1"
                     /translation="MKKGYIYTASLALMLALGFSKNGLSQNIGINTSGATPDASAGLD
                     ISFTDKGLLIPRVNITNLNAASPITSPATSLLVYNTNATTGLGYYYWDGSRWVNLSTN
                     AWKITGNAGTNPTNNFLGTTDNQALVFRTNNTERMRVLANGNVGIGTSTPSQKLHVSG
                     GNLRLDGALMPNNNAGSANQFLLSSGAGTAPQW"
     gene            complement(8381..9130)
                     /locus_tag="PKN22_01180"
     CDS             complement(8381..9130)
                     /locus_tag="PKN22_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNS41344.1"
                     /translation="MATAAKKDTTVAEKLDALYELQKIDSEIDRIRTVRGELPLEVED
                     LEDEVEGLETRIVKIQEEIKEIEEEISDRKNATKDAEAAIAKFKEQQNNVRNNREFES
                     IAKEVEFQELEIKLHEKKSKEAKAKIAQKKITLDETKERLDFRKEDLKNKKSELDEIV
                     AETKKEEDELMAASDKAKAKIEDRLINAYVRLRQNAKNGLAVVPVDRESCGGCFNHIP
                     PQRQLDIEMKKKVIVCEHCGRILVPSIKEEN"
     gene            complement(9136..10233)
                     /locus_tag="PKN22_01185"
     CDS             complement(9136..10233)
                     /locus_tag="PKN22_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNS41345.1"
                     /translation="MLTVGEITDYLESLAPLSSQEDYDNSGLIVGSKNTPVTQALICL
                     DSTEEVIDEAIANGCNLVIAHHPIVFKGLKSLTGKNYVERVVIKCIQHNIALYAIHTN
                     LDNYRFGVNAEIGKRLKLTNLQVLAPKTNVLNKLVVFVPVEHHQTVANALFEAGAGEI
                     GNYSECSFYSQGTGTFTPSDAANPFIGKAKEASKVNEQKLEVLVSTHHLGQVVQSLKK
                     AHPYEEVAYDIFPLINSNAYEGGGMVGELETPMESTEFLKMVKTTFNCGVVRHTKLVQ
                     PTIQKVAFCGGSGAFLLKNAMAAKADIYITGDFKYHEFFDAEDRIIIADIGHFESEQF
                     TINLLSDIIIKKFPNFALRLTRINTNPIKYL"
     gene            complement(10233..10913)
                     /locus_tag="PKN22_01190"
     CDS             complement(10233..10913)
                     /locus_tag="PKN22_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNS41346.1"
                     /translation="MNRIKKDWNDIKINDSWAIFKIMSEFVEGYEKMAKIGPSISVFG
                     SARTPENDKYYQLAVEISQKLGEAGYGVITGGGPGIMEAGNKGAQQGGGKSIGLNIVL
                     PFEQFHNKYIDADSNLQFDYFFVRKVVFVKYSQGFVVLPGGFGTMDELFESLTLIQTK
                     KINKRPVVLIGTEYWSGLIDWIKKTMLQSGNISEKDLDFFKVVDTADDAVNYIDDFFK
                     THELKPNF"
     gene            11072..11680
                     /locus_tag="PKN22_01195"
     CDS             11072..11680
                     /locus_tag="PKN22_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41347.1"
                     /translation="MKTIKLTLVALFTGLLTVSLAQSVKVVEGSLATLKGSTSLNVEY
                     DWSDFRVGKFAKETEYLDKKEAEYNEKEAGKGTTWRASWNADKAGRYQPKFEELFNKA
                     TSKIGINLGNQADSKYLAIVKTTFLEPGWNIFVSKAPASANMDVTIVEKANPSKVVAK
                     VVITGSKGRTYGYGDLDTGVRIAECYALAGKKLGALFSKQLK"
     gene            complement(11737..12123)
                     /locus_tag="PKN22_01200"
     CDS             complement(11737..12123)
                     /locus_tag="PKN22_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41348.1"
                     /translation="MKISFTVFLVFFSVFCFGQTKVDGLKNKYLKTYKGTIPSYSILS
                     DTAIVRVDETPIEIQITKSEIKMTIGKLSKNGSYKVLFKGKSFYVLDAYFEGDITPER
                     IIVNERNKTIIREGTYPQPKTTLKAN"
     gene            complement(12152..>12402)
                     /locus_tag="PKN22_01205"
     CDS             complement(12152..>12402)
                     /locus_tag="PKN22_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41349.1"
                     /translation="FGNVYSNFPKTQDFDQDQLLKISSKSFSFPFDVISFNLRENVND
                     KISLSWHLSSQEKNKIKKAFYSTSNQEALQTLKYLLNE"
BASE COUNT         3666 a         2418 c         2003 g         4315 t
ORIGIN      
        1 aaagcaatgc taactggtct tgggtaagcc aaacatcttg ctcgtccaaa cgaacatcta
       61 ccgaaatttt ttcgtcttca gttttgaaaa ttacaaattg agaattcgtg ttttgcattg
      121 gtttaaattt ttacatttca gcaaacaata aatccaaaac ttctgcttca gaagtacagt
      181 tgtctatttt tgaagcaacc ctatccgaaa attctaccaa ggtttcatta ggtaaaatgt
      241 ataactcttt ttgcccatta tgctcaagaa taatgtattc aaaaatattt tttcgatata
      301 gtttttctac aaccaaatca ttctcaaaat ttaatggaaa acttaaacct cttttatcca
      361 ttaggttgta ataagttgca tacaaatcaa aatttctaac attaggaatt agagtttttg
      421 ttattacttc ctttcgggtc tggtcatttg tccagtagtt attacttatg ttcataactt
      481 aaaagttttt attaaaaatt acatcaagat gatactgaat aaagtccttt tttggaaaat
      541 atcgttgtgg ttgaattagt gattgatatt caatcggggc aaaatatttt ccataataat
      601 cagtatcttt cttatttttt aatttatcag aaatcaaaat tttgtatttt tcatttatcg
      661 caatcagacc tttatcatag gccttatcat acaatgccga taaacaaatt ccattttcag
      721 gatttaatct ttcatcttca ttcttcgacc aaggaatgat atgacttgca aaaagcaatt
      781 cgggaatatc aataccagta atcgcacatt tccccgaata attagcaata actatttgtc
      841 taaaaacatt ttgatttact cttgttttta cttcacgtat tttagtttca cctttcaaat
      901 ccttcaggtc aaaaagtaaa tcttcaaatt tggtttcaat agtttgattt tctttttttg
      961 ctaaaatttg ctcactttca aaaatcaaca actctttatt tttagcaaat tcgtcccata
     1021 ttggttggca ttgttttatt ccaccgacca ttccttttac acctctactt tgatgataag
     1081 ggtcacaggc tgcaaaatta gttagacgaa ttgcaattga atttacagtt cgacccaata
     1141 aatttgccat ttcaataatc tctggtgttc tggtgtgcat tttgccaaaa ggtaatttca
     1201 aatacagatt aaaaaccagt ataagctcat cttttgtcca gagttttctt tccatttttt
     1261 gaaaattaat tctattagtt tttattactt tttttctaaa taaaaccacc gttagtttga
     1321 aaagtcttaa tattttaatt ttttaaaaaa tattcaacaa ttcatccttt catcgttgta
     1381 aaactgttcc aatatagtta ttttttctcc aacatcaaca aaaaaagcgc tcagtttccc
     1441 gaacgctttt ccattataaa tagtcttgtt tacaatccta aaagtacttc gtatggatct
     1501 tttcctccaa acaacattct ttctggattt tctaacattt cttttacttt tactaagaaa
     1561 ccaaccgatt ctcttccgtc aatcacacgg tggtcataag aaagtgccaa atacatcatc
     1621 gggcgaattt ctactttacc gtcaatagcc acaggacgct caacgatgtt gtgcataccc
     1681 aaaattgccg attgcggtgg gttgatgatt ggtgttgaca tcatggaacc aaaaacccct
     1741 ccgttggtaa tggtaaacgt tcctcccatc atatcgtctg gtgtgatttt tccatcgcgt
     1801 gctttaattg ccaaacgctt gatttcggct tcaatttctg ccaaactcat ttgctctgcg
     1861 tttcttacta ctggcaccat caacccttta ggtgatgaaa cagcaatacc aatatctgca
     1921 tagtcgtgga aaatcatatc gttgccatca atcatagagt tcacagccgg ataaagattc
     1981 aacgcctctg taactgcttt tgtgaagaac gacataaatc ctaaattcac ctcgtggtgt
     2041 tttgcaaaac gatctttgta ttttgcacgc aagtccatga taggtttcat atccacctca
     2101 ttgaacgtag tcaacatcgc ggtgtcgttt ttcacagaaa ccaaacgctc tgcaatttta
     2161 cgacgcaaca tcgacatttt ttctctgcgt tgctcgcgtg taccgcccca accttgaaca
     2221 gcatctgttg caaaaccacc cgacattgct gcaagcacat cttgtttagt aatgcgtccg
     2281 tttttacctg ttccactaat tttagcaacg tttacaccgt tctcgttcgc aatttttgca
     2341 gcagctggcg atgaaactcc tgttgcgtaa ttttttgctg gttctggact tggcaccacc
     2401 gcttttgctt cttctttttt agcttctggc gctggcgttg caaccgtttc tttgttagcc
     2461 actggagtcg ctgccccttc tggtcgtgct gcagatgtat caatcaaaca aacaacttgt
     2521 ccaactttca ctacatcccc ttcacctgct tttaatgtta ttgttccact tgcttctgcg
     2581 ggcaattcca acgttgcttt gtcagaatcc acttctgcaa ttgcttgatc tttttctaca
     2641 taatcaccgt ctgatactaa ccattgagca atttctactt ccgaaatcga ctcacctgga
     2701 cttggtacct tcatttctaa tactgacatc gtaattggtt tttatatttc taaaattttt
     2761 attctctctt atgcttttac agctgcatat tgaaacaagt tgttccacaa agcattcaaa
     2821 cgttttaagt gcaattcttt agagccttct gccgctgctg ctgctgccgg acgcgtaacg
     2881 ccaattaaat ctttgcctct catgcgcatt gccatatatt gccaagcacc catgttttct
     2941 ggttcttctt gtgcccaaac aatgcgtttt ttgtttttat attttgccaa taccgcttca
     3001 atttgttttt ctgggaaagg atacaattgc tcaacacgca caaacgccat gttgtcaact
     3061 cctaactcag ctgctttttc ggttgcttcg taatagaatt tacccgtaca taaaactacc
     3121 gtttctatat tagcggcttt tgcttgagga tcatcaatta cttcttggaa accacctttt
     3181 gccatgtcat ccaacgacga aacagcagtt gggtaacgca acaacatttt tggagagaac
     3241 accaccaatg gttttctaaa agaacgactc aattgtcttc tcaacaagtg gaaatggttg
     3301 gctggcgttg atgtgtttgc aacttgaata ttcaaatcgg ctgcttgttg taagaaacgc
     3361 tccattcttc ccgaagagtg ttctgcacct tgcccttcgt atccgtgagg caacaacatc
     3421 accaaaccac tttgcaccga ccatttgtct tcacctgccg tgataaattg gtcaaccata
     3481 atttgagctc cgttgtagaa atcaccaaat tgagcttccc aaattgttaa tccttttgga
     3541 gatgccaatg aatatccgta ttcaaatcct aataccgcat attcactcaa caatgaattg
     3601 taaattgaaa aacgagcttg tttatcgccc aataaattca acgttacaat ttcttcttct
     3661 gtatcttctg ttttaacaac tgcatgacgg tgcgagaatg taccacgctc cacatcttga
     3721 ccagtaatgc gcaccgggtg accttcttct aacaacgttg cataagccaa catttctgca
     3781 ctaccccaat ccaattgatc ggttgtaatg gcttctaaac gatcgctaaa gattttttgg
     3841 atttttctga agtacgtctt cccttctggt aaggttgcta tctttttacc caattccaac
     3901 aattttttct ttggaattga tgtatcaaca gattgctcaa aatctgctgc agtagctgtt
     3961 ttcatacctt tccattcttc ttccatgaag ttgaaaacgg ttgctttttc ttgttttgca
     4021 gaagcatcaa attcaatttc taaaaagttt tgataggcat caattttctt tttagcgtct
     4081 tcgcgtgaca aaacaccttg cggaattaat gtttcaatgt ataattcact tgggttttta
     4141 tgttttgcaa ttgctttgta caagtttggc tgcgtaaact tcggttcatc tccttcgtta
     4201 tgaccatatt tacggtaacc caacaaatca ataaaaatat cttgtttaaa ctcttgacgg
     4261 taggcaacag caatttctac tgcttgaatt accgcctcaa catcatccgc atttacgtgg
     4321 aatacaggtg ctaatgttgt tttagcaatg tctgtacaat aaatagacga acgcgagtct
     4381 gtaaaattcg ttgtaaaccc aacttgattg ttggtgataa tgtgaatggt tccacctgtt
     4441 ttgtaacctt tcaatgccgc catttgaata acttcataaa caacaccttg tcctgccaca
     4501 gctgcatcac catgaatgat tactggacaa actttgttta ggtcgttgct atatttagca
     4561 tccaacgtag cacgcgataa accttgcact acaggaccca cagcttctaa gtgagaagga
     4621 tttgggcaaa gcgttaggct tacttctttt ccagacttgt ttacttttgt ggtatatccc
     4681 aagtggtatt tcacatcgcc gtcaaaatct tcagcatcaa attcttttcc tttgaattcg
     4741 ttgaaaatat ctttcggacg tttttcaaaa atattggtca atgtatttaa acgaccacgg
     4801 tgtgccattc ccataatgaa gctttccact cctttctcag cacctttgtt gatgatggtt
     4861 tttaatgcag gaattaaagc ttcaacccct tcaattgaaa aacgtttttg acccacgaat
     4921 tttttagcta aaaagccttc aaattcagag gcttgcatca attgcgagta aaactctact
     4981 ttttgttcgt tggtaaatgt tggacgattg gccaattcga tggcattcaa catccatttt
     5041 actttttgag gattgttcat gtacatgaat tcaatcccaa ttgattggca ataagttttt
     5101 tgtaaatgat ctacaattgc acgaagagtt gttgcaccca atcctacttc ttcacctgct
     5161 ttgaaaacag tgtctaaatc gctgtcttct aaaccaaagt ttgataattc caacgttggt
     5221 tcatattggc gacgttcacg aacaggattc gttttggtaa acaaatgtcc acgtgtacgg
     5281 taaccattaa ttaggtttaa aacttttaat tctttttgga aattttcggg cacatcttca
     5341 tcgtaatttg ccttagaaaa ttcaaatcct tcaaaaaatc gtttccaccc aatatcaaca
     5401 ctctcagggt cttgtcgata cattttatac agatgatcga ttgcgtttac atctgcattt
     5461 ccaacatacg aaatattatc catgtttaat gatcatttaa cagaaaggca aagttaggta
     5521 tattaattta ttgaacgaga aaaagtagca atattttgtg aatttttaaa aaatagtgga
     5581 taaaactatc agattaatga ttaaatgcaa tttataaaaa taaaaaaact gcccattgct
     5641 ggacagttct tcgaatttta aaatatatag ttagtcaata atgaccttta caggggtttg
     5701 caaggcttca tcaccaccca acaatttaac aaaataagca ccaggcacca cttctaaatg
     5761 agtgataggc accaaggttg ttcccgaaag tgcattaaca ttaactgaat aaacaacttt
     5821 tccagccaaa tcatatactt gcacggctcc gttttgtatt tcggaagaag aatgaatggc
     5881 tactacaaac gatccagagt tggggctagg ataaacttgt aggctgttct cagaagcttc
     5941 acacgaaaca gaaattggtc cgtaaatttg ttgttgacca tcaaaattga ttaaacgcaa
     6001 gcggtaatag gttgttgacg cccacgagcg gttatcttca taataatagt ttaacaattg
     6061 gtgggagttt cctgcagcgt ttacttcgcc aacatattcc caatctgtat cattattgcg
     6121 tttttcaact acaaacttat ccgagttttg ctcagaagct gttgaccaat tcaattggat
     6181 tttttgttca caattggcgt taaatcctgc cagtgtaatt ggcaatggat gcgatacgtc
     6241 caccaacacc caacttttaa agaaattggc tggagaaatc accgcattgt tgacttgttt
     6301 agtagccgta ttcacagttc caaacaacaa atcttgccaa cttccaacag atggtgcacc
     6361 aacgcaataa tttcctgcgc ctgcacctgg attgaagcgt tgtgcttgca aattactttc
     6421 tgtaattgtg ttggcagggg ctgcttcgtt aacaaaatca tagccaaatg acaaggaaac
     6481 ggctggacgt gtggcataag ccgctgggtc aattctccaa aaacgatcaa ccgcatacaa
     6541 cgaagcgtcc attgggccaa cagaagaaca catgttattc acatccgaag gatagggcgt
     6601 gttgttgttt gcagtgcgat acgttgaaaa aatcaaatta ccagttgcac tacctgctgt
     6661 tgtaacatca acaattgcag gcactttaat tccgttggta ttggtaaacg gtacatgatg
     6721 aacatccgta ttgttaccaa tattccattt cacaacatcg ttttcgtttt ccgataagat
     6781 gttgccacca ctccccaaaa gtgttatcgc attggcagca ccgttgtcaa caaccaaata
     6841 cgcttgattt tctatgttga tataagcgcc gttattgaac accaagcggg cttgtgcttg
     6901 tgcaaaaatg ggaagtagta gtagtattga aagtaacgtt ttcattgttt atttttttca
     6961 ttaataataa acttaacatc agctttaact ggtgttagtg acaatttact aatatttgtt
     7021 tttggcacaa tttctttgtg attattaacc aatttcaatc tattattact atttgtatca
     7081 actataaata catttgcatt actatttgaa tgaaaagata attttgaagg gattggagtg
     7141 gcacttttat tatttatttg tttgtgtgta taatttgatt tagttaatgt aagacttaca
     7201 ttattagatt gactagttaa cgccaaattt acttggatcg gtttactttt ttccttattt
     7261 tgctgtgcat tagttaggtt aacaaccata aaaaatgcaa ataaaaaaca actagttttc
     7321 atatttatta gtggattgct aaaactgtaa ttggaatact tcctgttatg tcataagggg
     7381 aatcatttaa aacattaact attatttgac cagcatctac aacaacattt ctaatcgtta
     7441 ttccttggta taacaaagga tttaaattac agtcaaaatt aattgaaata gctgatgaat
     7501 aggtaccaca taaagcatct ggatctgtaa ttgtaaaagt catatcagtt ccagaatacc
     7561 atgttcctga ccaagatagg ctggaataat actttccaaa tgatacaatt gaggccgcgt
     7621 tatcaatagt aaaattctac cattgaggtg cagtacctgc ccctgaagat aataaaaact
     7681 gattggcaga tcctgcattg ttattaggca tcaatgcacc gtctaaacgc aaatttccac
     7741 cagaaacatg tagtttttga gaaggtgtgc ttgtgccaat tccaacgttt ccattagcta
     7801 acaccctcat tctctccgta ttatttgttc taaacactaa tgcttgatta tcggttgtac
     7861 ctaaaaaatt atttgtaggg tttgtacctg catttcctgt aattttccaa gcatttgtgc
     7921 tcaaatttac ccatctagaa ccatcccaat aataatatcc caaacctgta gttgcgttgg
     7981 tgttataaac caataaactt gttgctggag acgtaattgg agatgctgca tttaagttgg
     8041 taatattcac acgaggaatt agcaaacctt tgtctgtaaa actaatgtct aaaccagcac
     8101 ttgcatcggg tgttgcacca gatgtattaa ttccaatatt ttgtgataaa ccatttttag
     8161 aaaaccctaa cgcaagcata agcgcaaggg aagcagtgta aatgtagcct tttttcatag
     8221 tatttaaatt atagtaccta acaacttact gttatatttt gattttaaaa agccttttta
     8281 taattatttt taacaaatcg gtttagtaag tccttttatc acaaaaaaag cacctatttt
     8341 tcaatagatg cttttcaaaa aaatgagggt atattttctc ttagttttct tctttgatag
     8401 aaggaactaa aatacgtccg cagtgttcgc aaacgattac ttttttcttc atttcgatgt
     8461 ctaattgacg ttgaggtgga atgtggttaa aacatcctcc gcaagattca cggtcaactg
     8521 gcacaacagc caaaccgttt ttagcatttt gacgcaaacg cacataagca ttaatcaaac
     8581 gatcttcaat ttttgctttt gctttatcag aagccgccat caattcgtct tcttcttttt
     8641 tcgtctcagc aacgatttca tccaactccg attttttgtt tttcaaatct tcttttctga
     8701 aatccaaacg ttctttggtt tcatccaaag taattttctt ttgagcaatt tttgcttttg
     8761 cttctttcga tttcttctcg tgcaatttaa tttctaattc ttgaaattca acctcttttg
     8821 caatcgattc aaactcacga ttgttgcgca cgttgttttg ttgctctttg aacttagcaa
     8881 ttgctgcctc tgcatctttg gttgcatttt tacgatcaga aatttcttct tcaatttctt
     8941 taatctcttc ttgaattttt acgatacgtg tttctaaacc ttccacttca tcctctaagt
     9001 cttccacttc taaaggtaat tcaccacgca ctgttctaat tctatcaatc tcagaatcaa
     9061 ttttttgcaa ttcgtaaaga gcatctaatt tttctgctac tgttgtatct ttttttgctg
     9121 cagttgccat atattttata aatatttaat cggattcgta ttaattcgcg ttaaacgaag
     9181 tgcaaagtta ggaaattttt ttattataat atcacttaat aaattaatcg taaattgttc
     9241 gctttcaaaa tgtccgatat ctgcaatgat tattcggtct tcggcgtcaa aaaattcgtg
     9301 gtatttaaaa tctccagtta tgtaaatatc ggcttttgca gccatagcgt ttttcaataa
     9361 aaaagcaccc gaaccgccac aaaatgctac tttttggata gttggttgca ctaattttgt
     9421 atgacgaaca acaccacagt tgaatgttgt tttcaccatt tttaaaaatt cggtgctctc
     9481 cataggtgtt tccaattcac caaccattcc tccaccttca taagcattgg aattgattaa
     9541 tggaaaaata tcataggcaa cttcttcata cggatgggct ttttttaagg attgcaccac
     9601 ttgccccaag tgatgagtgc tcaccaatac ttctagtttt tgttcattca ctttcgatgc
     9661 ttcttttgct tttccaataa atggatttgc tgcatcagag ggtgtaaaag tacctgtgcc
     9721 ttgactgtaa aaactacact cactgtaatt tccaatttcg ccagcacctg cttcaaacaa
     9781 tgcattggca actgtttgat ggtgttcaac gggaacaaat accaccaatt tatttagcac
     9841 attggttttt ggtgccaata cttgcaaatt ggttaatttt aatcgtttac caatttcagc
     9901 attaactcca aaacggtaat tatccaaatt agtatgaatg gcatacaagg caatgttgtg
     9961 ttgaatgcat ttgatgacaa cacgttctac ataatttttt cccgttagcg atttcaaccc
    10021 tttgaaaaca ataggatggt gcgctatgac caaattgcaa ccattggcaa tggcttcatc
    10081 tatcacttct tcggtactgt caaggcaaat taatgcttgt gtaacgggtg tgtttttaga
    10141 cccaacgatc aatccagaat tgtcataatc ttcttgactc gacaaggggg ccaaggattc
    10201 taggtaatcg gtaatttctc caactgttag cattagaaat taggttttaa ttcgtgtgtt
    10261 ttaaagaaat catcgatata attaacggca tcatcggcag tgtcaaccac tttgaaaaaa
    10321 tccaaatctt tttcagaaat attacccgat tgcaacatcg tttttttaat ccaatcaatt
    10381 aaacccgacc aatattcggt tccgatgagc actactggtc gcttattaat ttttttagtt
    10441 tgaattaagg ttagtgactc aaacaactca tccatagttc caaaaccacc aggcaaaacc
    10501 acaaatcctt gcgaatattt tacaaatacc actttacgta caaagaaata gtcaaattgc
    10561 aaattagaat cggcatcaat gtatttgttg tgaaactgtt caaaaggcaa aacaatgttt
    10621 aacccgatgg attttcctcc tccttgttgc gcacctttgt ttccagcttc cataattccg
    10681 ggtccgccac ctgttatgac gccatagcct gcttctccta atttttgaga tatttcaacg
    10741 gccaattggt agtatttatc gttttcaggg gtgcgtgccg agccaaaaac cgaaatactc
    10801 ggaccaattt ttgccatttt ttcgtagcct tctacaaatt cagacataat tttaaaaatt
    10861 gcccagctat cgtttatttt tatatcgttc caatcttttt ttattctgtt catattgttt
    10921 gttgttttat cagcaattaa aagtatttat tttttttgaa ttagaagaat atacgatgaa
    10981 tttcctattt tcttattttt tttcataatg aattaattct caatgttttt ttacagtata
    11041 tttgttttac cattaaatat ttaaaaaata tatgaaaaca atcaaattaa cgttagttgc
    11101 attattcact ggcttattaa cagtaagttt agcgcaaagt gtaaaagtag ttgaaggaag
    11161 tttagctaca ttaaaaggtt ctacttcatt gaacgtagaa tatgactggt ctgactttag
    11221 agtgggtaaa tttgctaaag aaacagagta tttagacaaa aaagaagcag aatacaacga
    11281 aaaagaggcg ggcaaaggta caacatggag agcttcttgg aatgctgaca aagcaggaag
    11341 ataccaacca aaatttgaag agttgttcaa caaagcaact tcaaaaattg gaatcaattt
    11401 aggaaatcaa gcagactcaa aatatttagc gattgttaaa acaacttttt tagagccagg
    11461 atggaatatt tttgtctcta aagctccagc aagtgccaac atggatgtaa ccattgttga
    11521 aaaagcaaat ccaagcaaag ttgttgctaa agttgttatt actggaagta agggaagaac
    11581 ttatggatat ggcgatttgg atacaggtgt gcgtattgca gaatgttatg cgttagctgg
    11641 taaaaaatta ggagctttgt tctctaaaca attgaaataa ttacacgtca ctatttttca
    11701 cttaaacaaa ggctgtttct ttagaaatgg ccttttttaa tttgctttaa gtgtagtttt
    11761 tggttgaggg taagtccctt ctctaatgat ggttttattt cgttcgttta ctataattcg
    11821 ttctggtgta atatctccct caaaataagc atctagcacg taaaaacttt tacctttaaa
    11881 caaaactttg tagcttccat ttttgcttaa tttaccaata gtcatcttaa tttcggattt
    11941 agtaatttga atttcaattg gcgtttcgtc aactcgaaca attgctgtat cacttaatat
    12001 ggagtaagag ggaattgtgc ctttataggt ttttaggtat ttgtttttta agccatccac
    12061 cttggtttgt ccaaagcaaa aaacagaaaa aaacaccaaa aatacggtga aactaatttt
    12121 catattgatg aagttgtgat tattgttcat gttattcatt caaaagatat tttaaagttt
    12181 gaagcgcctc ttgattggat gtgctataaa atgctttttt tattttgttt ttttcttgac
    12241 tacttaaatg ccaagataat gaaattttat catttacatt ttcgcgtaaa ttgaaactga
    12301 tgacatcaaa aggaaaagag aacgattttg aactaatttt taaaagctgg tcttgatcaa
    12361 aatcttgtgt tttggggaag tttgaataaa catttccaaa ag
//
LOCUS       DAOQVQ010000018        12274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5852_length_12274_cov_4.225714, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000018 DAOQVQ010000000
VERSION     DAOQVQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_12274_cov_4.225714"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4428)
                     /locus_tag="PKN22_01210"
     CDS             complement(<1..4428)
                     /locus_tag="PKN22_01210"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNS41350.1"
                     /translation="MLVFMFICTISLSQTPDVNGIYYVKPTATGTGTGDSWVNATADL
                     ESVIYYAGSGNKVFMAIGNYNVPTVSYKMKNNFEIYGGFDPGNGITNLTHSRILPSES
                     VEGTVFNGQGARRVFNNDFSFLSLNSSAVLDGVTIKNGYVNNDNGAGMRNINASPTLR
                     NIVVRNNNANNGAGGAIYNNNSSPSLTNVLILNNFSGANKAAYHSVNLSNSQITNSIF
                     KGNSQRLIHTESASLNMWNVLMADNPAATVALAQIGSGNTNIYNSTILNINGSNNAID
                     AFLSPGVINTINSIIYGNSNTATLNTCLYSLNLTTVPPSTVFVDYTNGNYQLRYDSPA
                     IDAGNNLLYSGLSASTKDLAGNPRVYQYSNGGIIDQGAYEFCIIPDATGIVYVKPTAT
                     GTGIGNSWANATSNFQGAIDATGTTKVFVAIGNYNAPLIGSFVMKNNVEIYGGFDPDN
                     GISTLSHSRIMPSATVNGSVMNGQNQVTVVRNVFASGNPLNNTAILDGFTITNGNRTT
                     TGYGGGIELQNASPILRNLVVKNNAVQDGGGAMGITQNSSPTISNIYLLDNTTPGISG
                     GLIIQSNSNPTFTNVYFKGNSASNSTHGSSIANFSGCSPTFINLMLTTTANSTLNSIS
                     TTNTSGGTGILTLINASIYTASSNAAGVVPAINSIAGATVNVRNSIVYGANSTATGGV
                     INLQSTQYYPTANTVLPTTIFVDPANYNFTLKEGSPAINVGDNSFYPGLSASTKDLAG
                     NARLHLYGMGGIIDLGPYESAYLQPDANGVVYVKPAATGTGSGDSWANATGNLQLAIN
                     TTGVQKVFVSVGWYVTPTNGFVMKNNVEIYGGFNPGSGITDLSHQRQFFLGSVLSGEN
                     TVRVINNDFLSTTALNTTAVLDGFTITRGYVSNDFGAGMLNRNASPTLRYITFLNNHA
                     VGTSGSGGAMANLNNSSPNLTNGSFTLNSADAQGAALYNASSSNGIFKTCQITNNECP
                     DGSALYAVNSSPTIMNSRLSENHGLNTVSINGSSSGITFINVTVANNTQVNAGVSPNV
                     LALGNGAVGAIKNSIVFGTISGAGSYTAQYSFIEGNSSTINGNVNTTGYTIADIYINP
                     TNGSYDLKGTAPVINKGSNALYTGLNSNSTDMAGNPRLVGAIIDLGATEFIVSPGGNG
                     IAYVKPTATGSGNGSSWANATADMQAAINATGTQKVFVAQGLYHVPSPSSYVMKNGVE
                     IYGGFDPDNGISDLTHNRIMPGLGNQGSILDGNNERPIIWNYNNGVTSSAKLDGFTLT
                     NAKGVNQGAIHNNAVSPTLTNLLIVNNDSYGIYNQSSSSTITNVAVLKNSGRGVHFSG
                     VGNATLVNATIAGNGGEAVYALNTDPMSNFSVVLNNSVVYGTVTNTLSQATANYSLVE
                     NNALSGNNAVDATGVSANQVFVDTLNNDFTLKGGAPAIGVGSNTLFTGLTASTRDLLG
                     NARLNGATIDLGAYEFKISVDANGIAYVKPTATGSGNGSS"
     gene            complement(4596..7133)
                     /locus_tag="PKN22_01215"
     CDS             complement(4596..7133)
                     /locus_tag="PKN22_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNS41351.1"
                     /translation="MKKSTILFLTSSLITLISNQVCAQTPNSNGVIFVKPVSTGTGDG
                     SSWENAMDNIQNAIDASGVSKVFVAIGNYPISTYTVMKNNVAIYGGFDPDNGIDDLTD
                     TRILPNVSINEGSVLDGGNTITPIRNYNNGLNNTAILDGFTIKNGRDIYGGAIYNSNA
                     AALYRNLVLKNNEATTSGGAIYNVNSPAVYIGVIIEGNTAQYGGGLQNVNSSPSFTNA
                     IIRNNTATMATAGAGGGAVFNQNSNASFVNCLIENNTTEFQGGAFVNLSGVPTLTNVT
                     IANNSATVDFSGLDVRGDSVIVNNSIIFGTNNGDISKKHSLVEGQNDTDNGNIDATSI
                     LASDIFVDEANGNYSLLLNSVALNAGEDTLFQGLNYSTTDIVSGKRKAGTAIDLGAYE
                     YQIFPSALGVAYVKPTATGTGLGNDWDNATDELQDAINATNVQKVFVSIGVYPLSSYS
                     LKMKNNVAIYGGFDPDNGIEELLDARILPNLSNDEGSVLDGLNSRRIIFNTYIDTSAI
                     LDGFTIKNGKSGHGAAIANQQSSPLLNNLVIKNNNATTSGGAIYNLNSNAIIQNTIIK
                     DNVALYGGGIRNNLSNSELTNVSIIGNSATMATTGAGGAGIFNELSSMKLTNVLIANN
                     TTEFQGGAFVNLSGVPTLTNVTIANNSATVDFSGLDVRGDSVIVNNSIIFGTNNGDIS
                     KKHSLVEGQNDTDNGNIDATGITATAIFADLVNNDFTVLETSPTIDAGNNELFINLSA
                     TTLDLAGNPRLNHAIIDLGAYEYDLTAGLNDLNLVQMSVYPNPTSSLLTINIPTSSRI
                     KIINMSGNVIYDNELNAGTHTIDVNNLVNGLYILQTDLGLNFKIIKE"
     gene            complement(7440..>12274)
                     /locus_tag="PKN22_01220"
     CDS             complement(7440..>12274)
                     /locus_tag="PKN22_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNS41352.1"
                     /translation="AFGIIYVKPTATGTGDGSSWTNATSDLHNAIHATGVSKVFVAIG
                     NFNVGTASFIMKNNVEIYGGFDPDNSIDDLTDSRILPTESITGSVLNGENNKPLIWND
                     NNGLTATAILDGFTLMNGKGGIAGAIYNNLVAPTYNNLVIRNNEATTAAGGIYNLNAP
                     ITLKNSIISNNTAPFAGGVRNNQSNAVFTNVIIKNNSATMSTGGSGGGGIFNESSDIE
                     LTNVLIANNSTNRWGGGFRNLSGNPVFTNVTLVNNTAVNQSATTAMEIVAGTPQFNNT
                     IVLGTISGTYTPQYSLIEGNTDFTNGNINPTGVTVTGVFTDPANGDYTLKFGSVAVNS
                     GNNALNATTTDLAGNTRVQNTTIDLGAYESAYAPISPDVNNILYVDINVTGGNGSGDS
                     WVNAIPQLAEALKYARLQYDANNTVYDTTPLKIYVAKGTYKPMYNAADNYYTTTTSVI
                     SGYDEIDNAFVMVKNVQIYGGFDPDNGIDDLTDTRIFGSNGSILSGDVAQTGATLDNA
                     RHVVISSDNVGNAVLDGFTIRDAFSLSGHSSAFITVNGYPVDRNKGAMVCVRSSPTIA
                     NCTFTKNYVSFLGGGITNYQQSSPTITNCVFKLNSAENGGAIANITGCSPIITNCSFI
                     DNGASVGGGAIYNTTSGSPTTTASSPIITNCSFTNNKSDDGGAIYNSSNSSPVIVNTL
                     FVNNIARGENYANDGNGGAIYNNASSPTITNVSIANNLGANAYYSTGTGSTTINNSIV
                     FGTISATYTPQYSLIEGNTDFTNGNINPTGVTVTGVFTDPANGDYTLKFGSVAVNSGN
                     NALNATTTDLAGNTRVQNTTIDLGAYESAYAPISPDVNNILYVDINVTGGNGSGDSWE
                     NAIPQLADALKYARLQYNADNTVYDAVPLKIYVAKGTYKPLYSARNNYFTQTGSADPA
                     YSDQDNAFVMVKNVQIYGGFDPENNIDDLTDTRIFGSNGSILSGDIGDIYDINYPYIN
                     ANHVMISSGDVGNALLNGFTITRAFSESTNAMLTVFIYPVQDAFGAMYCVRSSPTVEN
                     CIFTDNHTSGGNMFNYQQSSPTIINCTFINNGTMGSGGAIYNSTNSSPTITNCSFTGN
                     SAYEKGGAIYNYDNSSPIITNCSFKNNQSGTNSVGNGGGAIYNSASSPIIANSLFANN
                     KAMGNNYANPNGNGGAIYNVGTSSPVLTNVTIADNLGATTVYSTGTGSTTFNNSIVYG
                     GISATYVPQYSLIEGNTDFTNGNINPTGVTVSDIFTNPTDSIYTLQYGSVAVNSGNNA
                     LNATTTDLAGNARIIESTIDMGAYEYICPSTIDFSNVIFSDSTLTYDGTIHSLVAQNV
                     PSTATITYEITDAASQTIVGNSATNAGVYTVDVTLSDPLFSCNTVIITATLTINKAAG
                     VITANSTQTHVYDGTVKNVVATLNHSECPLVYTPQQGYTDVGVYSVTVTAIETDNYLS
                     TTQTINLEITKAVFTGITFNDGTFNYDGNSHSIDVVGAPVGATVTYTITDSQNNTQAG
                     NSAINVGVYTVEAVISMANYEDLVLEATLTIQTDLGTKETDEIIEISIYPNPFNDVLK
                     ISNVEHVQSIVIYDLSNRLIKKLPASTELNLSDLSNESYILELIMENNSRKHFKVIKQ
                     "
BASE COUNT         3733 a         2342 c         2128 g         4071 t
ORIGIN      
        1 agatgagccg tttccagaac ctgttgctgt tggtttcacg taagcaatac cgtttgcatc
       61 tacacttatt ttaaattcat aagcacccaa atcaattgtt gctccattta atcgagcatt
      121 gcccaacaaa tctctggtag aagctgttaa tccagtaaac agtgtattgc ttccaacacc
      181 tatggctggc gctcctcctt tcaatgtaaa gtcattgttt aacgtgtcca caaacacttg
      241 atttgcagaa actcctgtgg catcaacagc attgttacct gatagtgcat tgttttctac
      301 caatgaataa tttgcagttg cttgcgacaa cgtattggta actgttccgt acacaacact
      361 gttattcaat acaactgaaa aattggacat cggatcggta tttaaagcat aaacagcttc
      421 acctccattt cctgcaatag ttgcattcac caacgtagca tttcctactc ctgaaaaatg
      481 aacgcctctt ccactgtttt ttagaactgc aacatttgta attgtagagg aagaactttg
      541 gttataaata ccatacgaat cattgttcac aattaacaaa ttggttaatg ttggtgaaac
      601 agcattgttg tgaattgcac cttggttaac accttttgca ttggttagtg taaatccatc
      661 caattttgcg cttgatgtta caccgttatt atagttccaa ataattgggc gttcgttgtt
      721 accatccaat attgaacctt gatttcctaa tcctggcata atacggttgt gtgttaaatc
      781 tgaaatgccg ttgtctggat caaatccacc ataaatttca acaccatttt tcatgacata
      841 actacttggt gatggcacgt gataaagtcc ttgtgcaaca aatacttttt gtgttccagt
      901 tgcattgatt gctgcttgca tgtcagcagt tgcatttgcc caagatgagc cgtttccaga
      961 acctgttgct gttggtttca cgtaagcaat accgtttcct cctggactta caataaattc
     1021 agtagcacct aaatcaataa tagctccaac taatctagga tttcccgcca tatctgttga
     1081 atttgaattc aatccagtat acaaagcatt gcttccttta ttgataactg gggcagttcc
     1141 ttttaaatca tacgagccat ttgtaggatt gatataaata tcggcaattg tatatcctgt
     1201 tgtattaaca tttccgttta ttgtactgct gtttccttct ataaaagaat attgagcagt
     1261 ataagaacca gcacctgata ttgttccgaa aacaattgag tttttaatag caccaacagc
     1321 tccatttccc aatgcaagta catttggaga aacacctgca ttaacctgcg tattatttgc
     1381 aacagttaca ttgatgaatg taattccaga agatgagcca ttgattgaaa cagtattcag
     1441 tccatgattt tcgctcaatc ggctattcat aattgtaggc gaagaattta cagcatataa
     1501 agccgaacca tctggacatt cattatttgt tatctgacat gttttaaaaa taccattgct
     1561 tgatgatgca ttatataaag cagctccttg agcatcagca gaatttaaag tgaacgaacc
     1621 atttgttaaa tttggtgacg agttattcaa atttgccatt gctcctccag aaccagaagt
     1681 acctacagca tgattattca aaaatgttat gtatcggagt gttggagagg catttctgtt
     1741 taacattcca gctccaaaat cattgctaac atatcctctg gtaatggtaa atccatctaa
     1801 aacagcagta gtgttcaacg ctgtagttga aaggaagtcg ttattaatta cacgaacagt
     1861 attttctcca ctaagtacag aacctaaaaa gaattgtcgt tgatgtgata aatcagttat
     1921 cccagaacct ggattgaatc cgccgtaaat ttctacgttg tttttcatta caaaaccatt
     1981 ggttggcgta acataccagc ctacagatac aaaaactttt tgcacaccag ttgtgttgat
     2041 tgctaattgc aaatttccag tagcatttgc ccagctgtct ccactacctg tcccagttgc
     2101 tgctggtttt acatatacga caccatttgc atcaggttgt aaataggcac tttcatacgg
     2161 acctaagtca atgattccac ccataccata caaatgtaaa cgtgcatttc ctgctaaatc
     2221 ttttgttgag gcactaagtc ccggataaaa ggagttatct cccacgttaa ttgcaggcga
     2281 accctctttt aaagtaaaat tataatttgc tggatcaaca aaaatggttg ttggaagcac
     2341 cgtgtttgcg gttggataat attgtgttga ttgcaaatta atcactccac ctgtagcagt
     2401 agaattggct ccataaacaa ttgaatttct tacatttaca gttgcaccag caatagaatt
     2461 aatagcagga acaacaccag ccgcattgct acttgctgtg taaatacttg cattaattaa
     2521 tgtcagaatt ccagttcctc cactagtatt tgttgtagaa attgagttta atgttgaatt
     2581 tgcagtcgtt gtcagcatta aattgataaa cgttggtgaa catccgctaa agttagcgat
     2641 tgaggaacca tgtgttgaat tactagctga gtttccttta aaatacacat tagtaaaagt
     2701 tgggttggag ttggattgga taatgagtcc tccactgata cccggagttg tattatccaa
     2761 aagataaata ttgctaattg ttggagatga attttgtgta attcccattg ccccacctcc
     2821 atcttgtacg gcattgtttt ttacaacaag gtttcgcagg ataggagaag cgttttgaag
     2881 ctcgatacca ccaccatatc cagttgttgt tctatttcca ttggtaattg taaaaccatc
     2941 taaaattgct gtgttattca atgggttacc cgatgcaaac acatttcgaa caacagttac
     3001 ttggttttgt ccgttcataa cagaaccgtt tacagttgca gacggcatta ttcggctgtg
     3061 acttaatgtt gaaattccat tatctggatc gaaacctcca taaatctcca cattgttttt
     3121 catgacaaag cttccaatta atggtgcatt gtaatttcca atagcaacaa atacttttgt
     3181 agttccagtt gcatcaattg caccttgaaa attagaagta gcgtttgccc agctgtttcc
     3241 tatacctgta ccagttgcag ttggtttaac atatacaatt ccagtagcat caggaattat
     3301 acaaaattca taggcccctt gatcgatgat acctccatta ctgtactgat aaactcttgg
     3361 atttcctgct aaatcttttg tacttgcact taatcctgaa tacagtaaat tgtttcctgc
     3421 atcaatagca ggagaatcat acctaagctg gtaatttcca ttagtataat caacaaaaac
     3481 agttgatggt ggaactgttg taaggtttaa tgaatacaaa caggtattca atgtagcggt
     3541 atttgaatta ccatagataa ttgagttaat tgtattaata actcccggac ttaaaaaagc
     3601 gtctattgca ttgtttgaac cattaatatt taaaattgtt gagttataaa tatttgtatt
     3661 tcctgaacct atttgtgcta atgcaacagt tgctgctgga ttatcagcca ttaacacgtt
     3721 ccacatattt agtgacgcac tttctgtgtg aattaaacgc tgactgtttc ctttaaaaat
     3781 agaattagta atttggctat tactgagatt tacactatga taagccgctt tatttgctcc
     3841 tgaaaagtta tttaaaatca aaacattggt caatgaaggt gaggaattat tattgtaaat
     3901 tgctccgccg gctccattgt ttgcattgtt gtttcttacc acaatgtttc tcaaggtagg
     3961 cgaagcattt atatttcgca tacctgcacc attatcatta ttgacataac cattttttat
     4021 ggtaacacca tccaaaacag cagacgaatt taatgataga aatgaaaaat cattattaaa
     4081 aactcgtctt gctccctgtc cattaaacac agttccttct actgattctg aaggcaaaat
     4141 acggctgtgc gtcaagttcg taattccgtt tccaggatca aaaccaccat agatttcaaa
     4201 gttatttttc atcttataac tcactgtagg cacattgtaa tttccaattg ccataaacac
     4261 tttgtttccc gacccagcgt aatatattac actttctaaa tcggcagtag catttaccca
     4321 actatcccct gtaccggtac ccgttgcagt tggttttaca tagtaaatac cattaacatc
     4381 cggagtttgc gacaatgaaa tagtgcagat aaacataaaa actagcatta ttgttaatct
     4441 gcttaataaa ataaatttgt taatcattat tctcatttta cattttttac aatttgaaac
     4501 gaaggtattt aacttcattt atgcatctaa aaattaagcg ttacaaaagc gttacacctt
     4561 atttaaactc acaacaaata ctgataatca atgagttact ctttaattat tttaaaattc
     4621 aagcccaaat ctgtttgaag aatatacaga ccgttcacca agttatttac gtcgattgtg
     4681 tgtgtacctg cattcaattc attgtcataa atgacatttc ccgacatatt aataatttta
     4741 atacgagaag aagttggaat gtttatcgtt aataatgaac ttgttggatt cgggtaaacg
     4801 ctcatctgaa ctaaatttaa gtcgtttaaa ccagctgtta aatcatattc atacgcacct
     4861 aaatcaatta ttgcatggtt caatcttgga tttccagcta aatcaagtgt agtagctgaa
     4921 agattaataa acaattcatt attacctgca tcaatagttg gtgatgtttc aaggacagta
     4981 aaatcattgt ttacaaggtc tgcaaaaata gctgttgcag taattcctgt tgcatcaata
     5041 tttccattat cggtgtcatt ttgcccttca actaaggagt gttttttact aatatctcca
     5101 ttattggttc caaaaatgat tgaattattt acaattacag aatcgcctct tacatctaaa
     5161 ccagaaaaat ctaccgttgc cgaattgttg gcaatagtaa cattcgttaa agttggaaca
     5221 ccacttaagt tgacaaatgc tcctccttga aattctgttg tattatttgc gattaaaaca
     5281 ttcgttaact tcatagatga caactcgttg aaaatacctg ctccaccagc tccagttgtt
     5341 gccatagttg cactatttcc aataatgctc acatttgtta gctctgaatt tgaaagatta
     5401 tttctgattc caccaccata taaggcaaca ttatccttaa taatcgtatt ttgtattatt
     5461 gcattagaat ttagattata aattgcacca ccagaagttg ttgcattgtt gttttttatg
     5521 actaggttat ttaacaatgg tgaagattgt tgattagcta ttgcagctcc atgacctgat
     5581 tttccatttt taatcgtaaa accatctaat atggctgatg tatctatgta tgtattaaaa
     5641 ataattcttc ttgaattcaa accatccaaa acagatcctt catcgttact caaatttgga
     5701 agaatacgcg catctaatag ttcctcaata ccattatctg ggtcaaatcc accatatatt
     5761 gcaacgttat ttttcatttt taagctataa gaagataaag gatatactcc aattgacaca
     5821 aaaacttttt gaacatttgt tgcattgatt gcatcctgta attcatctgt tgcattgtcc
     5881 caatcgttgc ctaaacctgt acctgttgcc gttggcttca cgtatgctac tcctaatgct
     5941 gatgggaaaa tttgatattc ataagcgcct aaatctatgg ctgttcctgc ttttcttttt
     6001 ccagaaacaa tgtctgttgt gctataattc aatccttgaa ataaggtatc ttctccggca
     6061 ttcaacgcta ctgaattaag taataatgaa taatttccat ttgcttcatc cacaaaaata
     6121 tcagaagcaa gtatgcttgt tgcatcaata tttccattat cggtgtcatt ttgcccttca
     6181 actaaggagt gttttttact aatatctcca ttattggttc caaaaatgat tgaattattt
     6241 acaattacag aatcgcctct tacatctaaa ccagaaaaat ctaccgttgc cgaattgttg
     6301 gcaatagtaa cattcgttaa agttggaaca ccacttaagt tgacaaatgc tcctccttga
     6361 aattctgttg tattattctc gattaaacaa ttaacaaacg aagcattgga attttgatta
     6421 aaaacagcgc ctcctcctgc acctgcagtt gccattgttg cagtattgtt acgaattatt
     6481 gcatttgtaa aacttggact ggaattaaca ttttgtaaac cgcctccata ttgagccgta
     6541 tttccttcaa ttattactcc aatatataca gccggtgaat tcacattata gatagcacca
     6601 ccagaagttg tagcttcatt gttcttcaaa actaaattgc ggtataaagc tgctgcattt
     6661 gaattataaa tggcacctcc ataaatatct ctaccatttt taatcgtaaa gccatccaat
     6721 attgcggtat tatttaatcc gttattataa tttcgaattg gagtaattgt atttcctcca
     6781 tcaagcactg agccttcatt tatactaaca ttgggtaaaa tacgcgtatc cgttaagtcg
     6841 tctattccat tatctggatc aaaacctccg taaattgcca cattgttttt catgactgta
     6901 taggttgaaa ttggataatt tccaatagcg acaaaaacct tgctaacacc agaagcatcg
     6961 atggcatttt gaatattatc catggcattt tcccaactac ttccatcacc tgtaccagtt
     7021 gaaacgggtt taacaaaaat aacaccattc gaatttggtg tttgcgcaca aacttgatta
     7081 cttattaaag tgattaagct tgaagttaaa aatagaattg ttgatttttt cattgcattt
     7141 ttttttcaaa attattttga actgaaaaaa tcaacaaaaa tggagcgtta caaaagcgta
     7201 tcaattaatt ttagtatatt aactcgctag ataatagctc actacatttt tgagaatcgt
     7261 aataaattaa cgataaaatt gaagtgcaat ctaaaacaac tctttttaaa attcaattta
     7321 gatagaacta tcaggtgcgt aataagaatg cttgctactc ttaatataaa aaaataagtg
     7381 gaagcaatct actcccactt acttttgtta agttcttact aaaaataatt tctagcctac
     7441 tattgcttta ttactttaaa gtgctttcta gaattgtttt ccataatgag ttcaagaata
     7501 tatgattcat tactcaagtc tgatagattc aattctgtag aagcaggtaa ttttttaatc
     7561 aatctattcg acagatcata tatgacaatt gattgaacat gctcaacatt ggaaattttt
     7621 agaacatcgt taaatggatt tggataaatt gaaatctcaa ttatttcatc agtttccttt
     7681 gtgcctaaat ccgtttggat agttaaagtt gcttccaata ccaaatcttc ataatttgcc
     7741 atactaatta ctgcctcaac tgtataaacg cccacattta ttgctgaatt acctgcttgt
     7801 gtattgtttt gagaatctgt aattgtatat gttacagttg caccaactgg agcaccaact
     7861 acatcaatgg aatgtgaatt tccatcataa ttaaatgttc cgtcgttaaa cgtaataccc
     7921 gtgaaaacag ctttggtaat ttccaagtta attgtttgtg ttgttgatag gtagttatcc
     7981 gtttcgatag cagtaaccgt aacagaataa acaccaacat ctgtatagcc ttgttgtggt
     8041 gtgtaaacca atggacattc agaatgattt aaagtagcta caacattttt tacagttcca
     8101 tcgtaaacgt gggtttgtgt gctattggcg gtaataactc cagccgcttt attgatggtt
     8161 aaagttgcag ttataattac ggtattacag ctaaataaag gatcagacaa cgtaacatca
     8221 actgtataaa cacccgcatt tgttgcagaa ttacctacaa ttgtttgact tgctgcatcc
     8281 gttatttcat aggtaattgt tgctgtagaa ggaacatttt gtgcaactaa actgtggata
     8341 gtaccatcat aagtcaatgt gctatcacta aaaataacat tgctgaaatc aatcgttgat
     8401 ggacaaatgt attcataagc ccccatatca atggttgatt caataattct agcgtttcct
     8461 gccaaatctg tggtagtagc attgagtgcg ttattacctg aatttacagc aactgaacca
     8521 tattgcaagg tataaatgct atccgtagga ttggtaaaaa tatcacttac agtaactcca
     8581 gtaggattga tatttccgtt ggtgaaatca gtatttccct ctattaaaga atattgcgga
     8641 acataagtcg ctgaaattcc tccgtaaaca atggaattgt taaacgtggt gctacccgta
     8701 cctgtggaat aaacggtagt tgcacccaaa ttatcggcaa tggttacgtt ggtgagtaca
     8761 ggcgatgaag ttcctacgtt atagattgca ccaccgtttc cgtttggatt agcgtagttg
     8821 ttgcccattg ccttattatt ggcaaataat gaattggcaa taattggcga tgaagcggaa
     8881 ttgtagattg caccgcctcc gttgcctacg ctgtttgtac ctgactgatt gtttttaaaa
     8941 ctgcaattcg tgatgattgg cgaagaatta tcgtagttat aaattgcacc gcctttttcg
     9001 taagcactat tgcctgtaaa actgcaattg gtaatggttg gtgaagaatt ggtactgtta
     9061 tagattgcac caccgctacc catagttcca ttatttataa acgtacaatt gataatggta
     9121 ggcgaagatt gctgataatt aaacatatta ccgccagaag tatgattatc tgtaaaaatg
     9181 caattttcga cagttggcga agaacgaacg caatacattg ctccgaaagc atcttgaacg
     9241 ggataaataa agacggtaag catagcattg gtactttcag aaaaagctct tgtaatggta
     9301 aatccgttca acaaagcatt tcccacatcg cccgaagaaa tcataacgtg gttggcgttg
     9361 atgtaaggat aatttatatc ataaatatca cctatatcgc cacttaaaat agaaccgttt
     9421 gagccgaaaa ttcgggtatc ggttaaatcg tcaatgttgt tttcggggtc aaaacctccg
     9481 taaatctgca cattttttac cattacaaag gcgttgtcct ggtcgctata agctgggtca
     9541 gccgagcctg tttgtgtaaa ataattattt cttgcactat acaaaggttt gtaagtgcct
     9601 tttgctacgt aaatttttag aggtacggca tcataaaccg tattatcggc attgtattgc
     9661 agtcttgcgt atttcagtgc atctgcaagt tggggtatgg cgttttccca gctgtcgcca
     9721 ctgccatttc cacctgttac gttgatatcc acgtaaagaa tgttgtttac atcggggctt
     9781 attggagcat aagcactttc ataggcaccc aaatctatgg ttgtattttg aacacgtgta
     9841 tttcctgcca aatcggttgt ggtagcgttc aatgcgttat tacctgaatt tacagcaact
     9901 gaaccaaatt tcaaggtgta atctccgttt gcggggtcgg taaaaacacc cgttacagta
     9961 actccagttg gattaatgtt tccattagta aaatcggtat ttccctcgat tagagaatat
    10021 tgtggggtgt aagttgctga aattgtgccg aaaacaatag aattgttaat agtcgtgctt
    10081 cccgtgccgg tggaataata tgcattggca cccaaattat tggcaatgct tacgttggta
    10141 attgttggcg aagaagcatt attataaatg gctccgccgt taccatcatt tgcataattt
    10201 tcgcctcttg ctatgttatt gacaaataag gtgttgacaa ttacaggaga agaattagag
    10261 ctgttataaa ttgcacctcc gtcatcggat ttattattcg taaaactaca attggtgata
    10321 atgggcgaag aagctgttgt tgtaggcgaa ccactcgtag tattataaat tgcaccaccg
    10381 ccaacagacg ctccattgtc tataaaactg caattggtaa tgatgggtga acaacctgta
    10441 atattagcga ttgcccctcc attttcagcc gaatttagtt taaaaacaca attcgtaatt
    10501 gttggcgaag attgctgata attcgtaatc cctccgccca gaaaagagac atagttcttt
    10561 gtaaaagtgc agttggcaat agttggggaa gaacgaacac aaaccattgc acccttattt
    10621 ctatcaacag gataaccatt gacggtaata aatgcactgc tgtgacctga aagagaaaag
    10681 gcatctctaa tggtaaatcc atccaacaca gcattgccta cattatcgga agaaatgaca
    10741 acatgtcggg cgttgtctaa agttgctcct gtctgtgcta catcgccact taaaatagag
    10801 ccgtttgagc caaaaattcg ggtgtcagtt aaatcgtcaa tgccgttatc agggtcaaaa
    10861 cctccgtaaa tctgcacatt tttcaccatt acaaaggcat tatctatttc atcatagccg
    10921 gatattacag atgtagttgt ggtgtaataa ttgtcagccg cattgtacat tggtttgtaa
    10981 gtaccttttg ctacgtagat tttcagcggt gtggtatcat acacggtatt gttggcatcg
    11041 tattgcaatc tagcgtattt cagagcttct gccaattgcg gtatggcatt tacccagcta
    11101 tcaccactac catttccacc tgttacgttg atatccacgt aaagaatgtt gtttacatcg
    11161 gggcttattg gagcataagc actttcatag gcacccaaat ctatggttgt attttgaaca
    11221 cgtgtatttc ctgccaaatc ggttgtggta gcgttcaatg cgttattacc tgaatttaca
    11281 gcaactgaac caaatttcaa ggtgtaatct ccgtttgcgg ggtcggtaaa aacacccgtt
    11341 acagtaactc cagttggatt aatgtttcca ttagtaaaat cggtatttcc ctcgattaga
    11401 gaatattgtg gggtgtaagt gcccgaaatt gtgcctaaaa caatggtgtt attaaattgt
    11461 ggtgtacctg ctacaatttc cattgcagtt gtggcagatt ggttgacagc cgtgttattt
    11521 accaaagtta cattggtaaa aacaggattt cccgataggt tgcggaaacc tccaccccaa
    11581 cgattggtgc tgttattggc aatgagtacg tttgttaatt caatatcaga agattcattg
    11641 aaaattcctc caccaccaga gcctccagtt gacattgttg ctgaattatt cttaataatt
    11701 acattggtaa atactgcatt tgattggtta tttcgcactc ctccagcaaa aggagctgta
    11761 ttgttagaaa taatcgaatt ttttaaggta attggagcgt taaggttata aatacctccc
    11821 gcagcagttg ttgcctcatt gtttcttata accaaattgt tataggtagg cgcaaccaaa
    11881 ttgttgtaaa ttgctcctgc aatacctccc ttaccattca tcaatgtaaa gccatctaaa
    11941 atagctgttg ccgtcaaacc attgttatca ttccaaatga gcggtttatt gttttctcca
    12001 ttaagcaccg aacctgtgat gctttctgta ggtaaaatac gactatctgt taagtcgtca
    12061 atactattat caggatcaaa accaccgtaa atttccacat tgtttttcat gataaatgat
    12121 gcagtaccaa cgttgaaatt cccaatagct acaaaaactt tggaaacgcc tgttgcgtga
    12181 atagcattgt gtaaatcaga agtggcattc gtccaactac ttccatcacc tgtacctgtt
    12241 gcagttggtt ttacgtaaat aataccaaaa gcat
//
LOCUS       DAOQVQ010000019        12120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_5931_length_12120_cov_4.600580, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000019 DAOQVQ010000000
VERSION     DAOQVQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 12120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12120
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_12120_cov_4.600580"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="PKN22_01225"
     CDS             <1..1796
                     /locus_tag="PKN22_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNS41353.1"
                     /translation="GAKDDIAIVTFNNLFKDKGYSIEQVRNATLTEFDSCIKKEVSFL
                     SKRKLRSKYKNENDTYNLFQAVDDYCLQDVNATWLLYTKIQENFKKMIACFESSEMFS
                     ENIYNYISPSHASGVLLNNHLAKTGYRLSFTNENFFNFINDSFFGGRVDFGCIGEYLV
                     DDKIACLDVTSMYSLAMTANFPIIEVDEDVQISCLAEMSHVEFAHLIQQKVDHGNCLK
                     QLWIARCEITLPKDKTKLCSFGPLPERYNGKLEFPNKTFESKIITSVHAYTAMLCGYT
                     IRILPDPYNLYFKKSAPCLKDLVMEIIRLKAEAKEDNKALGKLLKLFANSFHGKLAQK
                     VIAKYHETRRYVNKTINNRFESMTISQSRIYLATFVTSFANLILMQAFLTIEENYIKD
                     KIPVEERAGTLLYCDTDSIFFRLKDEQVLTKFTISESLGSFNAETNTFDSTWSRKYNR
                     ANGIMIMGKKSYFVMETKNDKVTMLDLKLKGVHRGEMEQFTRQVVVDKIIKPFVAGFD
                     SQGISIERKTLKRKLLQPISMVEDNDGEQISFVGLERRANVFHSYEGNSSQSFVKSIY
                     ETILKKTLRVNVSDMKQVLKKVDDKLIFYLS"
     gene            complement(1784..2014)
                     /locus_tag="PKN22_01230"
     CDS             complement(1784..2014)
                     /locus_tag="PKN22_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41354.1"
                     /translation="MTKTSETITNKPRRKTNLEIIEQQRQAIRDKEERMQHLILHTLE
                     RYREKSKAKDKFDNKFYYEILNYVLKNKNQDK"
     gene            complement(1974..2471)
                     /locus_tag="PKN22_01235"
     CDS             complement(1974..2471)
                     /locus_tag="PKN22_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41355.1"
                     /translation="MSLYDQSKGLSNLELENYFTKHNIPFNEVDNMNKLNNPRTKQSI
                     VYTGTQPDAINNGVTKHWTYYSPFNKSKKELFDSYGDPTVYNQQFLNKNNINFVNRRQ
                     FQAFDTNVCGEYCAAWAKFLNEHPQSSVLPSSHLVEEFAQKHNLTKNRIQNDKNIRDY
                     YQQTA"
     gene            complement(2471..4225)
                     /locus_tag="PKN22_01240"
     CDS             complement(2471..4225)
                     /locus_tag="PKN22_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41356.1"
                     /translation="MATGGPLLTSQSLNPGTAAATGLAPSETLPNYKNDELLTVTSSS
                     GTFANEFNEDLTIVPDAIRTSYYKINPTAAPNGSNVLYNFRTQSWFNPSEMTITIPMQ
                     FTFWNMTSTTHTQITGANGAAALNALAYNSVNTTCFEYMWNKPIAEWLRSITVFQCRA
                     GNSAFLVQSYSNDQAPNIFSNVFIPSQREPLDSIESNIYLGNARNSRNGCVATFTAPS
                     TISTAGTFVADAINQLDQQCLLTDTIPSLQNALLNAIIKSATLNTPIELTFPIKMFLN
                     MFNTTKTTLFPPNFSFGFFINFNTQPFIVRTLGDFGIKAQVILNGANISLPFLTVNKA
                     EFSAEGTLALNNRLISKYINKNYFETKTIPPSNAIIPANSSSWTSQLTIASIRPVMYA
                     IYFIVPNVTDGAMYSIAAARINQDFQNSPVPNIYIQSLNLNIGGYQYVYLDRQNSQNN
                     YLWNIRDADDIQQSKINCRYMDYSVFHNQTTKSIECGVPFIFVIDPSTYVSEKQIGTI
                     QGPVTTNITVNFQELNTVTNTLTNTTRQLQAVCVEILHSQYTIYPTLDLNIVPISAKL
                     SGKAPNVNLPFVNRAVAA"
     gene            complement(4300..5745)
                     /locus_tag="PKN22_01245"
     CDS             complement(4300..5745)
                     /locus_tag="PKN22_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41357.1"
                     /translation="MARLVDTFSSELLDPFNSKKTCLNNFSSYKNNGYSPIIKPFSNY
                     DHLKFSFVLQHPVHVKEFKFLIPMTVNYNGNAIEETFFSNQFELDPITSKVSNFALSF
                     NNVRVNTVSNFCSNLNSILYRPTNHFERACFGDGVCRYNARNSTNGITSQPVLTGTYY
                     FHNKMNKQIIVPVPLGIFCDLFNQDVVLPDNTLIEIEFDSQLTPISRTIADNFAISAA
                     AKITIDNTWRWNQCQLIVPQRLVARENLNSLETKFFGKYREFMPLWSSFLNPSQQSAL
                     ELASKQKITIPLQHVPEFLVLWVNYGVYAPKVLDDGNAKITDIFGFTGNTVGRIFANE
                     ANPSSLAVDTVMDLKVENLFNEIISLSIDLNGTKILDYDYKRHRQSLSMYLVDDLIHG
                     QLPDKEMMDVQTRAPYIINLATSAVDQSTGTKITGGTLSIAVVFSRTFLRNTFTSSYF
                     AGTPSYSFNVWGAMNKKIQIDSWRRTFVESF"
     gene            complement(5733..7172)
                     /locus_tag="PKN22_01250"
     CDS             complement(5733..7172)
                     /locus_tag="PKN22_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41358.1"
                     /translation="MSKLVDTFSSDLLNPFNSIKTCLNNFSGVIFDNYLPTYQNLSDN
                     TRPYEFTIDLNHPTHVRDIFISYRLLSPLGFAFSPILCSVNEVDSIGRRFENFQIYLN
                     NIQLNTNHGYSQSLNSFFKKDDMTNCEEGVEGKLYQDNRSILYNYTTVLQGGPTLGTP
                     ASQLNWRRTYDGRISLGHFCNFFDQDTVLPENTRIRLSFNHSNSPGFNLQYGIIFPDN
                     YIFSTGSQSIEGSTFDYSWIPRILIFRRVIRRENIHKAELMFKCKWREFLPWQAILLD
                     GINNANSAEPRFYGKFTKFFQQIPEFLSFFISLGQWLPFSPVTSWTRVAQVITLKSGV
                     TFQYGNLALNSSTTNPISRFLYLDNARYFRQLRINVNGVQVFDYDYDASGLELDYYIK
                     SEQDDFNSNPNFTYDNLLKTKLFFINLTNKAGQQDTFSTLNGGEITVEYTIVDYLFPT
                     PINRNGQLHCWYSQRQSTQIDAYRRTWRD"
     gene            complement(7174..8781)
                     /locus_tag="PKN22_01255"
     CDS             complement(7174..8781)
                     /locus_tag="PKN22_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41359.1"
                     /translation="MSFLNQSSRLNAQFGNASVFRKETSTVHYQDNLRSKDLRLIAPV
                     QVTQLPVRTVHQSNNLAKKFQYTFEVTLEKFTRMSEIYLMLEGNLRAAPTTTTTFVGA
                     FLPQNRLLAPAFTLAQNFDNVEIMINNVAVTNPQPWCVMPGFLNTCEKNLAGLLHRPG
                     QPFQRLIQEGALGAAVTTRYMASDIDFDNTGTTLSTVQQTLQKMLVPISTFSNLFAQP
                     GILPPGTKLSFKATYQNCENWSAIVNTPPGITWVQATPPDLTAVQNWVFDTQTIHMQM
                     TYYNISSSLEKVFMEQWKTQPWVVRYFKTELMQFPTLQFRPTGGVPTVGQTITDRLEI
                     DSPANSCLLMCLALPKSAPDGYDTYPQEVYGGNQFCAYAYPTPSTTSTGNRLKMFPFK
                     FDYLKIFLEDNAEPIVDFTNLYANADLIHKFLKDETNKFFRINEHQCVTNTSDIYGYR
                     VAPFFLPLHSEKYFNPNFGPIPKRASYLISYQISSIEGTQINANFEVYFHIVRSSTLQ
                     IDSRLNTLVYDGPLEFNGDNIEDISKN"
     gene            complement(8875..9081)
                     /locus_tag="PKN22_01260"
     CDS             complement(8875..9081)
                     /locus_tag="PKN22_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41360.1"
                     /translation="MKRGRGCAVPFGGSRRRRGCGTAVPFGGSRKRRGGTAALAPFLL
                     ASALNNIKRKSQVASGRRRRHRRH"
     gene            complement(9318..11309)
                     /locus_tag="PKN22_01265"
     CDS             complement(9318..11309)
                     /locus_tag="PKN22_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41361.1"
                     /translation="MHKLKNYFSSEMPITIDEGSRYYLSGDVDKDFDTAVTIANELIQ
                     KIECLYSHDFSSKSADRNFPEEVKEQLKKCTADNLVDGKHHETLRLVQTGNSLDFTFE
                     RVNILTRTLPETIQMKVLLRVRKSIIKFCRMMANMSQHFDLDNTDPNLTGHIKLLEEL
                     TDGLDKFSFPKDYKVEKVTFVDGFVAQGKTSYIEEQGEVSYEFSQYFRIPLVESKQLM
                     TDKFYGLFSLWGFFVGFFNKKEEVILDRCPILQSAFSHENKNLVKISKLFLDNFFNLF
                     HVHVIKFKFFLYYNNMIPSSPADFSWKPTRQLEIQIYAQKDFRVEFQSHVFRKLFLFF
                     FLYPVPYFEIEKLSIPHYRAINLGNGFSIIPTLEIGTVKILMPMQFFFENNRVEYGEL
                     SPVPRRWIDCSDRTLTDFSMRGFDRSIPLKQTNIRRFLSTNNGARLLIDDSYNSECYF
                     YYNESSTQITLEFTTVPIILDLSGIKDSRIMNDLPVFDFKNDEQNAKLVGIRSNRSLD
                     DSIDIILSHLPYELAPLSQLSMLSLGIRFNPGHLRDSQWVLRSRYFHSLILRFIGDDM
                     VLENYSKEPVQLANRIFCYMPRFKVDLKVFSDRTDCGNALASVIFTPSSQPVGSSLVF
                     NSSTLFDKPLMKNLFNKYRIEFSSEEPDKKSKRTLRAGI"
     gene            complement(11302..>12120)
                     /locus_tag="PKN22_01270"
     CDS             complement(11302..>12120)
                     /locus_tag="PKN22_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41362.1"
                     /translation="TDFNYNNADITNVDFVAIPSTQPFVHVQASIGDFYLATKRIYAN
                     YLSDFPYTKSRNWAIAGYPDCAVGFEETATATSFLFTLSYFDGRTDRMNDYTVLTGSY
                     IPLLSYRNPADIKINLRMSGLDTFANGLRTNLINLNHYINMRIPSILTMNFYNIKVTD
                     LETTQIFSLGGLNPILIQSLNFLYCRVSINKQDYEVDPFNVLTGRVETNLIKFSNNNQ
                     NLYEPINYFNGVVSLSSWRQLTALTASSKYYYKIIIDSPIISDAASFNVQFCFA"
BASE COUNT         3945 a         1875 c         2225 g         4075 t
ORIGIN      
        1 gtggagcaaa agacgacatt gccatcgtta cctttaataa tttatttaaa gataaaggtt
       61 atagcattga gcaagttaga aatgcaacgc ttactgaatt tgattcgtgt atcaaaaagg
      121 aggttagctt tttatcaaaa agaaaattaa gatccaaata caagaatgaa aatgatactt
      181 acaacttgtt tcaagcagtt gatgactatt gtcttcaaga tgtaaacgct acatggttac
      241 tgtatacgaa aattcaagaa aatttcaaaa aaatgattgc ttgttttgag tcgtcagaaa
      301 tgttctctga aaatatttat aattacattt caccatcaca cgcttcggga gtgttgttga
      361 acaatcatct tgccaagact ggatatcggt tatcgtttac aaatgaaaat ttttttaatt
      421 ttattaacga tagctttttt ggaggacgtg tagattttgg ttgtattgga gaatatctcg
      481 tagatgacaa gattgcttgt ctcgatgtca cttcaatgta ttcactggca atgacagcaa
      541 attttcccat cattgaagtt gatgaagatg ttcagatttc ttgtcttgct gaaatgtcac
      601 atgtggaatt tgcacattta attcaacaaa aagttgatca tggaaattgt cttaagcaat
      661 tgtggatagc aagatgtgaa ataacattac ctaaagacaa aactaaactc tgttcatttg
      721 gaccgttacc tgagagatat aatggaaaat tagaatttcc caacaagaca tttgaatcaa
      781 aaataattac cagtgttcat gcgtatactg caatgttatg tggctatact attcgtatat
      841 tgccagatcc ttataattta tattttaaaa aaagtgcgcc atgtctcaaa gatcttgtca
      901 tggaaattat tcgattaaag gctgaagcga aagaagataa caaggcgttg ggaaaattat
      961 taaaattatt tgcaaatagt ttccatggaa aactagcaca aaaagttatc gccaagtatc
     1021 atgaaactcg taggtatgtt aacaagacca ttaacaatag atttgaatcc atgacaatat
     1081 ctcaatcgag aatttatttg gcgacatttg ttacgtcttt tgcaaactta attttgatgc
     1141 aagcattttt aactatagaa gagaattata taaaagacaa aattcctgtc gaggaaagag
     1201 ctggtacatt gttatattgt gatacagatt ctatattctt tagattgaaa gatgaacaag
     1261 tactaactaa atttacaata agtgaatcgt tgggctcgtt taatgctgaa acaaatactt
     1321 ttgatagcac ttggagtcgc aagtataatc gtgcaaatgg aattatgatt atgggaaaaa
     1381 aatcttattt tgtcatggaa acaaaaaatg ataaagtaac tatgctagat ttgaaattaa
     1441 aaggagtaca tcgcggtgaa atggaacaat tcactagaca agttgttgtt gacaaaatta
     1501 taaaaccatt tgtcgctggc tttgattcgc aaggtataag tattgaaaga aaaactctaa
     1561 aacgaaaact acttcaacca atcagtatgg ttgaagataa tgatggtgaa caaatttcat
     1621 ttgttggttt agaacgaaga gccaatgtgt ttcattcata tgaaggcaat tctagccaat
     1681 catttgtcaa gtctatttat gaaacaattt tgaaaaaaac attacgagtt aatgtttctg
     1741 atatgaaaca agtattaaaa aaagttgatg acaagttgat tttttattta tcttgatttt
     1801 tatttttcaa aacataattt aatatttcgt aataaaattt attgtcaaat ttatctttag
     1861 cttttgattt ttctctataa cgttccaaag tgtgtagtat caaatgctgc atacgctctt
     1921 ctttatctct aatggcttgt cgttgttgtt ctatgatttc taaattagtt tttctacgcg
     1981 gtttgttggt aatagtctct gatgtttttg tcattttgaa ttctattttt tgttagatta
     2041 tgtttttgag caaactcttc tactagatgt gatgaaggta gtactgagct ttgtggatgt
     2101 tcgtttaaaa attttgccca agcagcgcaa tattctccgc aaacgttggt gtcaaaagct
     2161 tgaaattgtc ttcgatttac aaagtttata ttgtttttgt ttaaaaattg ttgattgtat
     2221 actgtagggt ctccgtacga atcaaacaat tctttttttg atttgttaaa aggcgaataa
     2281 taagtccaat gtttagttac accattattt atagcgtcag gttgggtacc tgtgtacact
     2341 atagattgtt ttgttctagg attattaagt ttattcatgt tatctacttc attaaaaggt
     2401 atgttgtgtt ttgtaaaata attttcaagt tctagatttg aaagtccttt tgattggtcg
     2461 tacaaagaca ttatgcagct acagctcgat taacgaatgg aaggttgacg tttggagctt
     2521 ttcctgataa tttggcactg attggcacaa tgttaagatc aagagttgga taaatcgtgt
     2581 attgtgaatg taaaatttca acacaaacag cttgtaattg acgagttgtg tttgttaggg
     2641 tattagtaac tgtattcagt tcctgaaaat tgacagtaat atttgtcgta acaggacctt
     2701 gaatcgttcc aatttgtttt tcactaacat aggttgacgg gtcgattaca aaaatgaatg
     2761 gaactccaca ttcgatactt ttcgtggttt gattgtgaaa tacagaatag tccatgtatc
     2821 gacaattaat tttcgattgt tgaatatcgt cagcgtctcg aatattccac aaatagttgt
     2881 tttgagaatt ttgacgatca agatagacgt attggtatcc accaatattc aaattcaaag
     2941 attgaatata aatatttgga actggactat tttgaaagtc ttggttaatt cgagctgcag
     3001 cgatcgaata catcgctcca tctgtaacgt tgggaacgat aaaataaatc gcatacatta
     3061 caggtcggat tgacgcaatt gttaattggg aagtccaact cgacgaattt gcaggaataa
     3121 ttgcattcga tgggggaatt gtttttgttt caaagtaatt cttattaatg tattttgaaa
     3181 ttaatcgatt gttgagcgcc aaagttcctt ctgcggaaaa ttcggcttta tttactgtca
     3241 aaaatggcag cgaaatattt gctccattaa gaatgacttg agctttgatt ccaaaatctc
     3301 ctaatgttcg aacaatgaat ggttgcgtat taaaattgat aaagaatcca aatgaaaaat
     3361 ttggtggaaa taaggttgtt ttggttgtat taaacatgtt caaaaacatt ttaattggaa
     3421 acgtcaattc gatcggagtg tttaaagtgg ccgatttgat gattgcgttt aataatgcat
     3481 tttgaagtga aggaatcgta tcagtcaata aacattgttg atccagttga tttatagcat
     3541 cagcgacaaa tgttccagca gtactgatag tcgacggagc tgtaaaagtt gctacgcaac
     3601 cgtttctcga attacgagca ttgcccaagt atatgtttga ttcgattgaa tcgagtggtt
     3661 cacgttgact tggaatgaaa acgtttgaaa aaatatttgg tgcttgatca ttcgagtatg
     3721 attgaacgag aaaagctgaa tttccagcac ggcattgaaa caccgtgatt gatctaagcc
     3781 attctgcaat gggtttattc cacatatact cgaagcaagt tgtattaacc gagttgtagg
     3841 cgagtgcgtt taatgccgca gcaccgtttg ctccagtaat ttgcgtgtgt gtagttgatg
     3901 tcatgttcca aaatgtaaac tgcattggaa tggtgattgt catttcactt gggttgaacc
     3961 atgattgagt gcgaaagtta taaagaacgt tcgaaccgtt tggtgctgct gttggattaa
     4021 ttttataata actggtacga atagcgtcag gaacaattgt taaatcttca ttaaattcat
     4081 ttgcaaaagt tcctgacgaa gaagtgactg ttaaaagttc gtcatttttg taatttggaa
     4141 gtgtttctga aggagctaaa ccagttgctg cagcagttcc tggatttaag ctttgagaag
     4201 ttaaaagtgg tccaccagtg gccatgatta gattttaaaa aatgaaaatt tgattattgt
     4261 tttaagaata tttattgttc aagtattaat aaaaagttgc taaaatgatt caacaaaagt
     4321 tctacgccaa gaatcaattt gaattttttt attcatcgct ccccaaacat taaacgaata
     4381 tgaaggtgtt ccagcgaaat atgaagatgt aaatgtgtta cgcaaaaaag ttcttgaaaa
     4441 tacaactgct atcgataatg ttcctcctgt tatttttgtt cctgtcgatt gatcaacagc
     4501 cgatgttgcc aagttgatta tataaggagc gcgtgtttga acatccatca tttctttatc
     4561 gggcaattgg ccgtgaataa gatcgtcaac taaatacata gataacgatt gtcgatggcg
     4621 tttatagtca tagtcaagaa ttttagttcc attgaggtca atacttaatg aaataatttc
     4681 attaaacaag ttttcgactt taagatccat cactgtatcg acggcaagtg agcttgggtt
     4741 agcttcattc gcaaatattc tgccgacagt gttacctgta aatccaaaaa tgtctgtgat
     4801 tttagcgttt ccatcgtcta aaacttttgg agcatatact ccataattaa cccacaagac
     4861 taaaaattct ggaacatgct gtaaaggaat tgtgattttt tgtttagaag ccaattctaa
     4921 agctgattgt tgtgacgggt ttaaaaatga actccacagt ggcataaatt cgcgatattt
     4981 tccaaaaaat ttggtttcta gtgaattcaa attctcacga gctactaatc gttgtggaac
     5041 aattaattgg cattggttcc accgccaagt attatcaatg gtgatttttg ctgctgctga
     5101 aattgcaaaa ttatctgcaa ttgttcttga aattggtgtt aattgtgaat caaattcgat
     5161 ttcgataaga gtattgtcgg gtaataccac atcttgatta aataaatcgc aaaaaattcc
     5221 aagaggaact ggcactatta tttgtttatt cattttgttg tgaaaataat atgttcctgt
     5281 cagaacaggc tgagaagtaa ttccattggt actattgcgc gcgttatatc tacatactcc
     5341 atctccgaaa caagctcgtt caaaatgatt tgttgggcgg tataaaatac tatttaaatt
     5401 ggaacaaaaa tttgaaacag tgttgacacg aacattgttg aagctgagtg caaaattact
     5461 aacttttgaa gtgattgggt cgagttcaaa ttgatttgaa aaaaatgttt cttctatcgc
     5521 attcccgtta taattgactg tcataggaat taaaaattta aattctttta catgtacggg
     5581 atgttgaaga acaaacgaaa attttaaatg atcataattg gaaaaaggct taatgattgg
     5641 agaataacca ttatttttat aagatgaaaa attatttaaa catgtttttt tcgaattaaa
     5701 tggatcgagt aattccgatg aaaatgtgtc aactaatcgc gccatgtgcg tctataagcg
     5761 tcaatttgtg ttgattgtcg ttgtgaatac caacaatgca attgcccgtt tctattaata
     5821 ggggttggaa ataaataatc tacaattgtg tattctactg ttatttctcc accatttaaa
     5881 gttgaaaaag tatcttgttg tcctgctttg tttgttagat tgataaaaaa taattttgtt
     5941 ttcaaaagat tgtcatatgt gaaatttgga ttggaattga aatcatcttg ttcgctttta
     6001 atataataat caagttctaa tcccgaggcg tcgtaatcgt aatcgaatac ttgaacaccg
     6061 ttaacattaa ttcttaattg tcgaaaatat cgtgcattgt cgagatataa aaatcttgat
     6121 atcgggtttg ttgtcgatga atttaatgca agattgccat attgaaaggt aacgcctgat
     6181 tttagtgtaa ttacttgtgc aactcttgtc catgaagtca ccggtgaaaa aggtaaccat
     6241 tgacccagtg aaataaaaaa tgataaaaat tctggaattt gttgaaaaaa ttttgtaaat
     6301 tttccataaa atcttggttc tgccgaatta gcgttattga ttccgtctaa caaaattgct
     6361 tgccatggaa gaaattctcg ccatttacat ttaaacatca attcagcttt atgaatattt
     6421 tctcgacgta tgactctacg aaatattaaa atacgtggaa tccaggaata atcaaacgtt
     6481 gaaccttcta ttgattgaga accagttgaa aatatatagt tatctggaaa aataatacca
     6541 tattgcaagt taaatccagg tgaatttgaa tgattaaaac taaggcgtat tcgtgtgttt
     6601 tctggaagaa ctgtatcttg atcgaaaaaa ttgcaaaaat gtcctaacga aatgcgtcca
     6661 tcataagttc ttcgccaatt cagttgactt gcaggcgttc caagtgttgg acctccttgc
     6721 aatactgttg tgtaattgta taaaattgaa cgattgtctt gatataattt cccttccaca
     6781 ccttcttcac aattggtcat gtcatctttt ttaaaaaaag agtttaatga ttgactgtaa
     6841 ccatgattgg tgttgagttg aatattgttt aaataaattt gaaaattttc gaatcgtcgc
     6901 ccaatagaat caacctcgtt aacagaacat aatatgggtg aaaatgcgaa acccaaagga
     6961 cttaaaagtc tgtacgagat aaatatatca cgcacatgtg taggatgatt taaatcaatt
     7021 gtaaattcat aaggacgcgt attatcagat aaattttgat atgtaggtaa ataattatca
     7081 aaaataacac cagaaaaatt atttaaacat gtttttattg aattaaaagg atttagtaaa
     7141 tcagaagaaa aagtatctac gagcttagac attttaattt tttgaaatat cctctatgtt
     7201 atcgccatta aattccaaag gaccatcata tactagagtg ttgagtctag aatcaatttg
     7261 aagtgtcgac gatctgacga tgtggaaata tacctcgaaa ttcgcgttaa tttgtgttcc
     7321 ttcgatcgag ctaatctgat atgaaatgag atatgaagct cgtttaggaa taggtccaaa
     7381 gttcggattg aaatattttt cactatgaag tggtaagaaa aatggagcga ctcgataacc
     7441 gtaaatgtcg gaggtgtttg taacacattg atgttcgttt attctgaaaa atttatttgt
     7501 ttcatctttc aaaaatttat gaatcaagtc ggcattcgca tacagatttg tgaaatcgac
     7561 aataggttca gcgttgtctt ctaaaaaaat tttcaaataa tcaaatttaa atggaaacat
     7621 tttgagccga tttccagtcg atgttgtcga tggtgtggga tatgcgtaag cgcaaaattg
     7681 attgccgcca taaacttctt gaggataagt atcatatcca tcaggagcac tttttggaag
     7741 tgccaagcac attagtaaac atgaatttgc tggtgaatcg atttcgagtc gatctgtaat
     7801 agtttgccct acagttggaa ctcctcctgt tggacgaaat tgtaaagttg gaaactgcat
     7861 caactcagtt ttgaaatatc gaactaccca aggctgtgtt ttccactgct ccataaatac
     7921 tttttcaagc gatgaagaaa tgttgtaata agtcatttgc atatgaatcg tttgtgtatc
     7981 aaatacccag ttttgtacag ccgtcaaatc aggtggagtt gcttgaaccc acgtaatacc
     8041 aggaggcgta tttacgatgg cactccaatt ttcgcagttt tgatatgtgg ccttgaatga
     8101 aagttttgtt cctggcggta aaattcctgg ctgagcgaac aaattcgaaa aagtcgatat
     8161 tggaaccaac attttttgaa gagtttgctg aactgttgaa agagtagttc ctgtgttgtc
     8221 gaaatcaata tcactagcca tgtatctagt tgttactgca gctcccaaag ctccttcttg
     8281 aataaggcgt tgaaaaggtt ggccaggtcg atgtaacaag cctgctagat ttttctcgca
     8341 agtgtttaaa aatcccggca ttacacacca aggttgtggg tttgttaccg ccacattgtt
     8401 gatcataatt tcgacattat caaaattctg agccaacgtg aaagcaggtg cgagtaatct
     8461 attttgaggg agaaaggctc caacaaaagt agtagtagtt gtgggagctg ctcgcaagtt
     8521 tccttccaac attaaataaa tttcactcat gcgagtgaat ttttccaaag ttacttcaaa
     8581 tgtatattga aatttttttg caagattatt tgattgatga acggttctaa caggtaattg
     8641 agtaacctga actggagcga tcaatctcaa atctttcgat ctcaaattat cttgataatg
     8701 aacagttgaa gtctcttttc gaaataccga tgcatttcca aattgtgcgt ttaaacgtga
     8761 agattgattt aaaaaagaca tgtttatatt tttaaaaatt gtttattgaa ttattattat
     8821 cagcagtaag aacattaata aaaaacttaa tgtctaaaat tatattaaaa gcaattagtg
     8881 gcgtctatga cgtcttcgac gacccgaagc tacttgtgac tttcgtttga tattgtttaa
     8941 agcactggct aaaagaaaag gtgccaacgc ggcagtgcca cctcttcttt ttctcgatcc
     9001 tccaaatgga acagctgttc cacaacctcg tcttcttctt gatcctccga aaggaacagc
     9061 gcagcctcga cctcgtttca tgctattggg tatgcagaac ctgaaattac tcctctacct
     9121 cgacgtttaa ttttcttgcg accaagagct gataatacga ttggtgctac tgataataat
     9181 gtttgtccga tgtgtcctaa tactgatttg aagctaccgc ctcgtctgcc tttacttttt
     9241 ttatgcgttg attttcttga agatcttcgt tttttagcca taatttagcg gtcgattaat
     9301 ttctaattaa aatattttta aatacctgca cgtaaagttc gcttagactt tttatccggt
     9361 tcttcggaac taaattcgat acgatacttg ttaaacagat ttttcattaa cggtttatca
     9421 aaaagcgttg aactattgaa tactaaagaa cttcctactg gttgcgaaga aggtgtgaaa
     9481 attacacttg ccaaggcatt tccacaatcg gttcgatcag aaaaaacttt caaatcgact
     9541 ttgaatcgag gcatgtagca aaagattcga ttcgccaatt gtactggttc tttgctatag
     9601 ttttccaaaa ccatatcatc gccaataaat ctcaatatga gactatggaa atatctcgat
     9661 cttaataccc attgagaatc tctcaaatgt cctggattga atcgaattcc aagtgataac
     9721 attgacaatt gtgacaatgg cgcaagctca tagggaagat gtgaaagaat aatatcgatt
     9781 gaatcatcga gcgaacgatt acttcgaatt cccaccaact tggcattttg ttcatcgttc
     9841 ttgaaatcga acacgggcaa atcattcatg attcgactat ctttaatgcc actaagatcc
     9901 aatataattg gaacagtggt aaattcgagt gttatctgtg ttgacgattc gttataataa
     9961 aaataacatt ctgaattata agaatcgtca attaacagtc ttgcgccatt gtttgttgaa
    10021 agaaatcttc taatgtttgt ttgttttaat gggattgatc gatcaaatcc tcgcattgaa
    10081 aagtcagtca atgttctatc actacaatcg atccatcttc ttggtactgg cgataactct
    10141 ccatattcga ctcgattgtt ttcaaagaaa aattgcattg gcatcaaaat ttttacagtg
    10201 ccaatttcaa gagttggaat tatcgaaaag ccatttccta aattgatggc tcgataatga
    10261 ggaattgaaa gtttttcaat ttcaaaataa ggaacaggat aaagaaaaaa gaataaaaat
    10321 agttttcgaa aaacgtgtga ttgaaattca actcgaaaat ctttttgcgc ataaatttgt
    10381 atttcaagtt gacgagttgg tttccaagaa aagtctgcag gagaagaagg aatcatatta
    10441 ttataataca aaaagaattt gaacttgatg acatgcacat gaaataaatt aaaaaagtta
    10501 tcgagaaaaa gcttagaaat tttgacaagg tttttgtttt cgtgactaaa cgcactttga
    10561 agaattggac atctatctag aataacttct tcttttttgt taaagaatcc cacaaagaac
    10621 ccccagagag aaaataaccc atagaatttg tcagtcataa gttgtttcga ttctactaat
    10681 ggaattcgaa aatattgact aaattcgtac gaaacttcac cttgttcttc gatgtatgaa
    10741 gttttacctt gtgcaacaaa tccgtcaaca aatgtgactt tctcgacttt gtaatctttc
    10801 ggaaaagaaa acttatcaag tccgtcagtt aattcttcga gtaatttgat gtgtcctgta
    10861 aggtttggat cagtattgtc caaatcaaag tgctgggaca tgtttgccat cattcggcaa
    10921 aattttatga tcgatttgcg tactcgtaac aacaccttca tctgaatggt ttcaggaagt
    10981 gttcgagtta aaatgttaac tcgttcaaat gtaaaatcaa gagaattgcc cgtttgaacg
    11041 agacgcaacg tttcgtgatg tttaccatcg accaaattgt cagcggtaca ttttttcaac
    11101 tgctccttga cttcttcggg aaaatttcga tctgcggatt tgctcgaaaa atcgtgagag
    11161 tacaaacatt cgattttttg aatcaactca ttggcgattg tcacggcggt atcaaagtcc
    11221 ttgtcaacat ctccactcaa ataatatctc gaaccttcat caattgttat cggcatttca
    11281 gatgaaaaat aattttttaa tttatgcaaa acagaattga acattaaaac tagcagcgtc
    11341 tgaaataata ggagaatcta tgatgatttt atagtagtat tttgacgagg cggttaacgc
    11401 agttaattgt cgccaagatg ataatgaaac cacgccgttg aaataattaa ttggttcata
    11461 aagattttga ttgttgtttg aaaacttgat aagattagtt tctactcgtc cagttaaaac
    11521 gttaaatgga tcgacttcat aatcttgttt atttatactc acgcgacaat ataaaaaatt
    11581 taatgactga attaaaatag gattgagtcc tcctaacgaa aatatttgtg ttgtttctaa
    11641 atcagttact ttaatgttat aaaaattcat tgtcaatata gaaggtatgc gcatgttgat
    11701 ataatgattc aaattaatta aatttgttct aagtccattt gcaaaagtat ccaacccact
    11761 cattcttaaa ttaattttaa tatcggctgg attgcgataa gataacagag gaatataact
    11821 gcctgtcaaa actgtataat cattcattcg gtccgtgcga ccatcaaaat aagataatgt
    11881 aaataaaaaa cttgttgctg tggcagtttc ttcaaaacca actgcgcagt cagggtaacc
    11941 agctattgcc cagttacgac ttttagtata gggaaaatca ctaagatagt tggcatagat
    12001 acgtttcgtt gccaagtaaa aatctccaat tgaagcttga acatgtacaa atggttgtgt
    12061 tgatggtatg gcaacgaaat caacatttgt tatgtcagca ttattataat taaaatccgt
//
LOCUS       DAOQVQ010000020        11672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_6221_length_11672_cov_3.773522, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000020 DAOQVQ010000000
VERSION     DAOQVQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6221_length_11672_cov_3.773522"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKN22_01275"
     CDS             <1..807
                     /locus_tag="PKN22_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNS41363.1"
                     /translation="IWQDWKLTYEYNSMGKKIKKSKANVYFKTSFDPAGNFLSLFNKY
                     LDTNSLGQKTVFGLGYSQFVRLDNEVIYAQPIGKKHSFNMRFLAGAGMPYGNSRTSLP
                     YDYSFFGGGANDNRGWRSRALGPGVYKYYLDPHRTATQVGDIRIAASAEMRFTMSGIF
                     KGAFFVDAGNVWTWKNDVNRPGSQFTKDWYKQLAVSAGLGLRLDFDFFVVRLDLGLPL
                     SNPSLPKGSQWIFQSRQAYKDEVYATPGIDLSKVPKPFIPVLHFGIGYPF"
     gene            complement(811..2778)
                     /locus_tag="PKN22_01280"
     CDS             complement(811..2778)
                     /locus_tag="PKN22_01280"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNS41364.1"
                     /translation="MLKIKILAIALLALNVAIAQETSQNCAKKRARSNSLKISPYVDQ
                     TQIDLQKKYDVTFYRLQLNMTNTTTALSGVTEIHARATQAIDSALVEFYSGFTINQIQ
                     VNGTPVTTYARHGQVLYIPINLPQNSAFTISIDYAGTPPTAATNPLGGAGMTNDDSPT
                     WGNQVTWSLSEPFSAFEWFPCKQDLRDKADSVQVTVTVPDHLMAGSNGLLDSVTTTPG
                     YKTFHWMHRYPIAYYLISVAVSNYYDYTIYANPAGAAQPLMIQNFIYNNTTYLTQYQD
                     DIDETVDFIELFADLYGPYPFQNEKYGHCLAPLSGGMEHQTMTTQGFFNNTLTSHELA
                     HQWWGDYVTCASWADIWINEGFASYSENLMLQNLYSINDAKQEMSSIHTNVMSNPGGS
                     VWVADSLDEDAIFDSRLVYNKGGAIIHTLRYIINNDQLFFDLLKDFLATYAYSTATGL
                     DFKNHAENFTGMDFSDFFNQWYYGEGYPTYSVQYQPETTGVWVKVNQTVSKSSVTPLF
                     TNPIDVTVNRTGTLSDTIVRIAINSNATTVFLPIENVQSITAIDPNNYVINKIGGITQ
                     VNDLGVEQAQMENENTILYPNPTENQLTVVFPGEKTRELSIIDHRGQTVFSTTVTSNT
                     TVDVSHLAAGQYLVEIKGKNKNSVIRKVIKR"
     gene            complement(2920..3276)
                     /locus_tag="PKN22_01285"
     CDS             complement(2920..3276)
                     /locus_tag="PKN22_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNS41365.1"
                     /translation="MSKVKILHNKMCSKSCTAMNFLKDHGFSDADIDVNLYVENGIDT
                     ATAQLIVDTFEGNLEDLIRKPDAKKLEIEVPSPLTKEWIVENIVKTPKIMQRPIIIKD
                     GKAIIGRSEESLGMLI"
     gene            complement(3287..4369)
                     /gene="prfB"
                     /locus_tag="PKN22_01290"
     CDS             complement(3287..4369)
                     /gene="prfB"
                     /locus_tag="PKN22_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNS41366.1"
                     /translation="MVNQEQLKELAERVKAIENYLSIPAKRLELKEEELKTQDPEFWN
                     DAKKAEAKMKDIRKLKFWVEAFDKLTAKLGDLEVTLEFFKEEMATEEEVDAIAAELKE
                     NIEDLELKNMLSGEEDALNAVLQITAGAGGTEAQDWAEMLYRMYKMWADKNGYKTQVL
                     NYQEGDVAGIKTVTIELEGDHAFGYLKGENGIHRLVRVSPYNAQGKRMTSFASIYVYP
                     SVDDTIHIEINPADISWDTFRSGGAGGQNVNKVETGVRLKHAPSGIVIENTETRSQLD
                     NREKAMQLLRSQLYEIELRKRMESRNEIEAGKKKIEWGSQIRSYVLDDRRVKDHRTNY
                     TVNDPDKVLDGDIFPFLKSYLMEFGN"
     gene            4676..5200
                     /locus_tag="PKN22_01295"
     CDS             4676..5200
                     /locus_tag="PKN22_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41367.1"
                     /translation="MSNRFKLAKEEYKNEDENGRNRLVYAYYGLSIYYAQCLEETFLI
                     MLLVKRINNKEIHSTYTTHELIEMMETSKKTMGNFINEVTQNYKLPEKTIKQLEKTLE
                     LRNYLIHNYFKVHISKFSVPNGKLEMIKYFCEFIDNVVQLDDNLNKYYQVYIQRFGIT
                     QEQINLALNDLKKL"
     gene            complement(5195..5824)
                     /locus_tag="PKN22_01300"
     CDS             complement(5195..5824)
                     /locus_tag="PKN22_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41368.1"
                     /translation="MKKSRLFFVILVLFFSCNNSLISNDINYKIIETTENKNISKINI
                     KVLLPEKVNRKKLENIAYEIKETYSNFENIWISYFIPNMEINSVSWAFSNFTPNLEIE
                     IVGTDGRTDKINTNTSKIKGEILGKWQCNKSLMGAILVLYRKDTGDIIMRINWDAENF
                     TENVVSESLYKNLKRYDDNNDNGEFYLIENNNNLGMYGINGKFDEALKL"
     gene            complement(5821..6414)
                     /locus_tag="PKN22_01305"
     CDS             complement(5821..6414)
                     /locus_tag="PKN22_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41369.1"
                     /translation="MSYLFGLLFVIICVIIIVYFNNSKNKSKSLENETNFLDILNQRK
                     LQPDQILFTDYNNSSNFLSVNEKQKKLCYGFIKDEQINVNIFDFKDILDFDVQVNDES
                     KRKVSIGGAVVGAALAGGLGALIGSQSGNQVNKIKNINFLITVNNISQPTIKFLLLEP
                     TMDGKGWDPDGIVVKNAIHKAETWSGVFNIIFNENKR"
     gene            complement(6501..7595)
                     /locus_tag="PKN22_01310"
     CDS             complement(6501..7595)
                     /locus_tag="PKN22_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNS41370.1"
                     /translation="MPKYIYEQENWTAFCWDEKAINALFGEVRLMQGKIMGQLNSLGF
                     SQKEEVSLTALTLDVVKSSEIEGEILNYNQVRSSIARRLGINTAGLVSSNRHIEGVVE
                     MMLDATQRYDLPLTEERLFGWHAALFPTGYSGHYKIETAQYRTGEMQIVSGAMGKEKI
                     HYEAIKPQFVQQEMDHFIQWFNDDNTLDPVLKAAIAHFWFIIIHPFDDGNGRIARALT
                     DLLLAKAEKSGERFYSMSSQILNEHKQYYAILQKTQHSTNNITEWMAWFLNCLKNALE
                     TTEQSTQNILQKAAFWRKHESTPINERQRLMINKLFDGFDGKLQSSKWAKITKTSTDT
                     ALRDIKDLIAKGILQPTNDGGRSASYVMVE"
     gene            complement(7766..10393)
                     /gene="clpB"
                     /locus_tag="PKN22_01315"
     CDS             complement(7766..10393)
                     /gene="clpB"
                     /locus_tag="PKN22_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNS41371.1"
                     /translation="MDIQKFTLKSQEAIQQAELIATEKGHQAIENAHVLKGILNIDKD
                     VVPYLFSKLNVNTAIVEQTLDRILDSAAKVSGGGQLYLSSNAQKTLNNAFVEMKNLGD
                     EYVTIETIVYAMLDGSDNVAQLLKDNKITKKNFKEAIMDLRNGEKVTSNSQDSNYKSL
                     EKYAKNLNDLARQGKLDPVIGRDEEIRRVLQILTRRTKNNPILIGEPGVGKTAIAEGI
                     AHRIVNGDVPENLKSKIVYSLDMGALIAGAKYKGEFEERLKSVVKEVIASDGEIILFI
                     DEIHTLVGAGGGGEGAMDAANILKPALARGELRAVGATTLNEYQKYFEKDKALVRRFQ
                     TVLVDEPSAEDAISILRGIKEKYENHHKVLIKDAAIVAAVELSQRYITDRHLPDKAID
                     LIDEAASKLRMEINSKPEELDEIDRKIMQLEIEREAIKREKDVQKIEALEKELAELNA
                     QRDAFQAKWQSERNVIDAVQQAKEEIDTLEMQAQQAERAGDYGKVAEIRYGKIKEVEQ
                     KLDENKKKLAEIQAKSNLIKEEVDADEIAEVVAKWTGIPVSKMLESEVAKLLRLEEEL
                     EKRVVGQEQAIEALSDAVRRSRAGLQDKRKPIGSFIFLGTTGVGKTELAKALAEYLFN
                     DENAMTRIDMSEYQEKHAVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSIILLDEIEKA
                     HPDVFNVLLQVLDDGRLTDNKGRLVNFKNTIIIMTSNLGSNIIHEKFDGVSEKNYLKA
                     SEQAKKEVMELLKATIRPEFLNRIDETILFNPLTKGNVRQIVTIQLNNLKQLLTEQGI
                     TLVVTPEAFDWIADAGYDPFFGARPVKRVIQRSVLNELSKALLGGTIDKEHNVVMDIF
                     DGKIVFRKPINEKEESSVY"
     gene            complement(10528..10977)
                     /locus_tag="PKN22_01320"
     CDS             complement(10528..10977)
                     /locus_tag="PKN22_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNS41372.1"
                     /translation="MDKTQFLDFLTHFVQSKIDFLQQEISDIQRDNASDTKSTAGDKH
                     ETGRAMMHLEIEKLAHQVAEYQQQLQTIHHFKHLSTTTNCIANGTLIQLSTGWFFLGV
                     ALGKVTYENTPVFCLSQQAPLGQQLLGKKVGEDIKVNASTLRIVKIY"
     gene            complement(11101..>11672)
                     /locus_tag="PKN22_01325"
     CDS             complement(11101..>11672)
                     /locus_tag="PKN22_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNS41373.1"
                     /translation="TNKKQFTMSIHDLALKGKHNAQNSLASGIAARILEIRKETVRES
                     LSDFVNVEHRLEFVAKVHGIEFINDSKATNVNSTWFALESMDKPTVWIVGGVDKGNDY
                     TELMDLVKANVKAIVCLGVDNSKIIEAFTGVVDTIVEARSAMEAVAYGYRLAKKDETV
                     LLSPACASFDLFENYEQRGNLFKEAVRAL"
BASE COUNT         3365 a         2276 c         2019 g         4012 t
ORIGIN      
        1 atttggcaag attggaaatt gacctacgaa tacaacagca tgggcaagaa aatcaaaaaa
       61 tccaaagcca atgtttattt taaaacatcg tttgaccctg caggtaattt cttatcgctt
      121 ttcaataagt atttagacac caactctttg gggcaaaaaa ctgtttttgg attgggttat
      181 tcgcaatttg ttcgtttaga caatgaagtt atttacgcac aaccaattgg aaaaaaacac
      241 agtttcaaca tgcgcttttt ggctggcgca ggtatgccgt atggaaactc acgcacctcc
      301 ttgccttatg actattcatt ttttggaggt ggtgccaatg acaatagagg atggcgctca
      361 cgtgctttag gccctggagt ttacaagtat tatttagatc cgcaccgtac ggccacacaa
      421 gtgggtgata ttcgtattgc tgcatcggca gaaatgcgct ttaccatgag tggaattttt
      481 aaaggagcat tttttgtgga cgccggaaat gtgtggacat ggaaaaatga cgttaaccga
      541 ccagggtcgc aatttacaaa agattggtac aaacaattag ccgtttctgc tggattgggc
      601 ttgcgtttgg actttgattt ctttgtggtg cgcctcgacc tcggattgcc actttccaat
      661 ccttcattac ccaaaggatc gcaatggatt ttccaatccc gacaagcata caaagatgag
      721 gtgtatgcaa cacctggcat cgatttatca aaagtgccaa aaccgtttat tcctgtattg
      781 cactttggta ttggatatcc gttttaacgc ttatcgtttt attacttttc tgataacgct
      841 gtttttgttt ttccctttga tttcaaccaa atattgacca gcagctaagt gtgaaacatc
      901 caccgttgtg ttggaagtta ctgtggtgct aaaaactgtt tgtccgcggt gatcaatgat
      961 agataactca cgtgtttttt cgcctggaaa aacaactgtt aattgatttt ctgttggatt
     1021 gggatataaa atcgtgtttt cgttttccat ttgtgcttgc tccaccccta aatcgttgac
     1081 ttgtgtaatg cctccaattt tattgatgac ataattgtta gggtcaattg ctgtaatgga
     1141 ttgcacattt tcaatgggta agaaaacagt tgttgcattt gaattaatgg caatgcgcac
     1201 aatggtgtcg ctcaatgttc ctgttcggtt tacagtaaca tcaatagggt tggtaaacaa
     1261 aggcgtaacg cttgattttg ataccgtttg atttactttt acccaaacac cagttgtttc
     1321 gggttggtat tgcacactat aggttggata gccctcaccg taataccatt gattgaaaaa
     1381 gtcagaaaaa tccatgcctg taaaattctc cgcatgattt ttaaagtcca atccagttgc
     1441 agtgctatag gcataagttg ccaaaaagtc ttttaataag tcaaaaaaca gttgatcgtt
     1501 gttgatgatg tagcgcaacg tatgaatgat tgctccacct ttgttatata ccaaacgact
     1561 gtcaaaaata gcatcttcgt ccaagctatc tgccacccaa acgcttcctc cagggttaga
     1621 cattacattg gtatgaatag acgacatttc ttgttttgca tcgttaatag aatacaagtt
     1681 ttgcaacatc aaattttctg aataagaagc aaatccttcg ttgatccaaa tatctgccca
     1741 cgaagcacat gtaacatagt cgccccacca ttgatgtgcc aactcgtgtg atgtcaacgt
     1801 attgttaaaa aatccttgtg tggtcatggt ttggtgttcc attccacccg aaagcggcgc
     1861 taaacaatgg ccgtattttt cattttgaaa cggataaggt ccgtataaat cggcaaacaa
     1921 ctcaataaaa tcaactgttt cgtcaatatc atcttggtat tgtgttaaat aggtggtatt
     1981 gttgtagata aagttttgta tcatcaacgg ttgggcagcc cctgctggat tggcgtagat
     2041 ggtgtaatcg taataattag atacagccac cgaaatcaaa taataggcaa tagggtagcg
     2101 gtgcatccaa tgaaatgttt tgtatcctgg agtggtggtt acactatcca acaaaccgtt
     2161 tgaacctgcc attaaatgat cgggaacagt caccgtaact tgaaccgagt cggctttatc
     2221 tcttaaatct tgcttgcacg gaaaccattc aaatgctgaa aaaggttcag aaagcgacca
     2281 cgtaacttga ttgccccatg ttggtgaatc gtcattggtc atgcccgctc cacccaacgg
     2341 attagtggct gcagttggcg gtgttccagc ataatcaatg gaaatggtaa acgcgctgtt
     2401 ttgtggtaaa ttgatgggaa tgtaaagcac ttgtccgtga cgcgcatacg ttgttactgg
     2461 cgttccattc acctgaattt gattgatggt aaaaccacta taaaactcaa ccaatgcaga
     2521 atcgatggct tgagttgccc gtgcatgaat ttcggtaaca ccactcaatg ctgtagttgt
     2581 gttggtcatg ttcaactgca agcggtaaaa cgtaacgtcg tatttttttt gcaaatcaat
     2641 ttgtgtttga tcaacatacg gactgatttt caaagaattg gaacgtgctc ttttcttagc
     2701 acaattttga gaagtttctt gtgcaattgc aacattcaac gccaataaag caatagcaag
     2761 tattttgatt tttaacatag tttgtattta aaagttgttt tcaccaacaa acagtatttc
     2821 aggaaagaat ataattatcc taccacaaaa caaactgtca tttgtgcaac taaaagtagt
     2881 aaaaaaataa tgaaaacgat ttatagcgaa gaaaaaacat taaatcagca tacctaagct
     2941 ttcttccgaa cgaccaataa ttgcttttcc atccttgata ataataggac gctgcatgat
     3001 ttttggcgtt ttaacgatgt tttctacaat ccattctttg gtaagaggag aaggtacttc
     3061 gatttctaat tttttagcat cgggttttcg gattaaatct tccaaatttc cttcaaatgt
     3121 atccacaatt aattgagctg ttgcggtgtc aattccgttt tctacataca aattcacgtc
     3181 aatatcggca tcagaaaaac catggtcttt caaaaagttc attgctgtgc aacttttaga
     3241 acacatttta ttgtgtagta tttttacttt actcattgtt tgcttgttaa ttaccaaatt
     3301 ccattaaata cgatttcaag aaaggaaaaa tatcgccatc caacacttta tcagggtcgt
     3361 tgactgtgta gttggtgcgg tgatctttta cacgtctatc atcgagcaca taactgcgaa
     3421 tttgcgaacc ccattcaatt tttttcttac ctgcttcaat ttcgttgcgc gattccatac
     3481 gtttgcgcaa ttcaatttca tacaattgag aacgaagtaa ttgcatcgct ttctcacggt
     3541 tatccaattg cgagcgtgtt tctgtgtttt caattacaat tccagaagga gcgtgtttca
     3601 agcgcacacc cgtttccact ttgttgacat tttgtccacc agcaccaccc gaacgaaaag
     3661 tatcccacga aatatctgct gggttgattt caatatgaat ggtatcatcc accgaaggat
     3721 acacgtaaat cgatgcaaaa gaagtcatac gttttccttg cgcattgtag ggactcacac
     3781 gcaccaagcg atgaatacca ttttcgcctt ttaaatatcc aaaagcatgg tcgccttcca
     3841 gctcaatggt caccgtttta atacccgcca catcgccttc ttggtagttg agcacttgtg
     3901 ttttgtagcc atttttatcg gcccacattt tatacatgcg gtagagcatt tctgcccaat
     3961 cttgcgcttc agttccacct gctccggctg ttatttgcaa cacggcgttg agtgcatctt
     4021 cctcgcccga gagcatattt ttgagttcta aatcttcaat attttctttc aattcagcgg
     4081 caatggcgtc cacttcttct tcggtagcca tttcttcttt gaaaaattcg agggttactt
     4141 ccaaatcacc caatttagcc gttaatttat caaatgcttc tacccaaaat ttcaattttc
     4201 ggatatcttt cattttggct tcggcttttt tggcatcgtt ccaaaattcg gggtcttgcg
     4261 ttttaagttc ttcttctttg agttccaaac gtttagctgg aatagaaagg taattttcta
     4321 tcgcctttac acgttctgct aattctttta attgttcttg attcaccatt gcactacaaa
     4381 aataatgaaa gtgtgagttt tttagtagat gaggtgggaa aaaagatggg aaagttgttt
     4441 ggagtggagt aaaaaggtga tttaataatt attatattta ttgaaaattg taaattacca
     4501 ttattacatt ttgcctttta ctgatgtgag atggagagtg ttaaaattct gacaacttaa
     4561 gattctgaaa tagcaataca atcataaaat gatctattta tttttgtgtt cttaggcaat
     4621 gcaagctgta tttattatat ttacgtattg gggtaaatgt tatcaaatca tatatatgtc
     4681 aaaccggttt aaattagcaa aagaagaata taaaaatgaa gatgaaaatg gtcgtaatag
     4741 actagtttat gcatattatg ggctttcaat atattatgca caatgtttag aagaaacttt
     4801 tttaattatg cttttagtaa aaagaattaa taataaagag attcattcca cttatacaac
     4861 tcatgagtta attgaaatga tggaaacatc taaaaaaact atgggtaatt ttattaatga
     4921 agttacccaa aattacaaat tacctgagaa aacaattaag cagcttgaaa aaacacttga
     4981 attaaggaat tatttaatac acaattattt taaagtacat atttcaaaat ttagcgtacc
     5041 taatggaaaa cttgaaatga ttaaatattt ttgtgaattt atagacaatg tggtgcagtt
     5101 agatgataat ttaaataaat attatcaagt atatattcaa cgttttggta taacccaaga
     5161 acaaattaat ctagcattga atgatttaaa gaaattatag ttttaaggct tcatcaaatt
     5221 taccatttat accatacatg cctaaattat tattattctc aattaaataa aattcaccat
     5281 tgtcattgtt atcatcatat ctttttaagt ttttgtataa ggattcagat acaacatttt
     5341 cagtaaaatt ttcagcatcc caatttattc tcattataat atcacctgta tctttcctat
     5401 aaagcactaa tattgctccc attagacttt tattgcattg ccattttcct agaatctcac
     5461 cttttatttt acttgtgttt gtatttattt tatctgttcg accatcagta ccaacgattt
     5521 caatttccag atttggagtg aaatttgaaa atgcccatga aacagagttg atttccatat
     5581 tgggaataaa ataagagatc cagatatttt caaaattact atatgtttct tttatttcat
     5641 atgcaatgtt ttctaatttt tttctattaa ctttttcagg taaaagaact tttatattaa
     5701 tttttgaaat atttttattt tcagtagttt ctattatttt ataattaata tcatttgata
     5761 ttagggaatt gttacaagaa aaaaataaaa caagtattac aaaaaatagt cttgattttt
     5821 tcatctcttg ttttcattaa aaataatatt gaaaactcca gaccatgttt cagctttatg
     5881 aatagcattt ttaactacaa tcccatctgg atcccaacct ttaccatcca ttgtaggttc
     5941 taataacaaa aacttaattg ttggttggct tatattatta acagtaatta aaaaattaat
     6001 attttttatt ttatttactt gatttccaga ttggctccca attaaagccc caagaccacc
     6061 tgctaatgca gcgcctacta cagcgcctcc gattgaaacc tttcttttac tttcatcatt
     6121 tacttgcaca tcaaagtcaa gaatgtcttt gaagtcaaag atattgacat taatttgttc
     6181 gtctttaatg aaaccataac ataatttttt ttgcttctca ttaacactta agaaattgct
     6241 actattattg taatcggtaa ataaaatttg atcgggttgt aattttcttt ggtttaatat
     6301 atctaaaaaa ttagtttcat tttctaaaga ttttgattta tttttagaat tgttgaaata
     6361 tacaataata attacacaaa taattacgaa taaaagtcca aaaagatagc tcatattata
     6421 tttttgattt caaatatata atattttttt aataaatttt tatacagctt tttttaaaca
     6481 aaagttatat cctcaaacca tcactccacc atcacataac tagcgcttct accgccatcg
     6541 ttggtgggtt gcaaaatacc ttttgctatt aagtctttaa tatctcttaa agcggtatct
     6601 gttgatgttt tggtgatttt tgcccatttt gatgattgca atttaccgtc aaagccatca
     6661 aataatttat taatcatcaa acgttggcgt tcgttgatgg gtgtggattc atgtttgcgc
     6721 caaaatgctg ccttttggag tatgttttgc gtggattgtt ctgttgtttc tagagcattt
     6781 ttcaagcaat ttaaaaacca cgccatccat tctgttatat tgtttgtact gtgttgtgtt
     6841 ttttgcaaaa tagcgtagta ttgcttgtgt tcgttgagaa tttgactcga catgctgtaa
     6901 aaacgttcgc cacttttctc tgctttggcc aacaaaaggt cggtaagcgc tcttgcaata
     6961 cgtccatttc catcatcaaa agggtgaatt atgatgaacc aaaaatgagc aattgcagcc
     7021 tttaatactg gatccaacgt attatcatca ttaaaccatt gaataaaatg atccatttct
     7081 tgttgcacaa attgtggttt gatggcttcg taatggattt tttctttacc cattgcaccc
     7141 gaaacaatct gcatttcacc tgtgcggtat tgcgcagttt caattttgta atgcccacta
     7201 taacctgttg gaaaaagtgc tgcatgccaa ccaaataatc gttcttcagt aagcggtaaa
     7261 tcataacgtt gcgtggcatc tagcatcatt tcaacaactc cctcaatatg acgattactg
     7321 gaaacaagtc cagcagtgtt aatgcctaaa cgacgagcaa tggacgaacg aacttggttg
     7381 taattaagaa tttcaccttc aatttctgac gattttacaa catcgagtgt aagtgcagtt
     7441 aaagaaactt cttctttttg tgaaaacccc aatgaattca gctgccccat gatttttcct
     7501 tgcatcaaac gcacctctcc aaacaaagcg ttaattgctt tttcgtccca gcaaaatgct
     7561 gtccaattct cctgctcgta gatgtatttt ggcatattaa attcttatta tgcggtaaat
     7621 atacaactta ttcaccgcaa aaatgcggta aatagcaact tttttcaccg catatatttt
     7681 taacacacat aaaatcaatg tgataaaaaa caaagaaggt tgcccgaaat tacttcagac
     7741 aaccttcttg ttttatagaa ctaaattaat aaacgctact ttcttctttt tcgttgatgg
     7801 gttttctaaa cacaattttg ccatcaaaga tatccatcac tacgttgtgt tctttgtcga
     7861 tggtgccgcc caacaatgct ttcgacaatt cgttgagaac agaacgctga atgacacgtt
     7921 ttactggtcg cgcaccaaag aatgggtcgt agcctgcatc ggcaatccaa tcaaatgctt
     7981 ctggcgtaac caccaaggta atgccttgct ctgtcaacaa ttgttttaaa ttattgagct
     8041 gaatagtaac aatctgacgc acatttcctt tggtcaacgg gttgaatagt attgtttcat
     8101 caatacggtt caaaaactcg ggacgtatgg ttgctttcaa caattccatc acttcttttt
     8161 tggcttgctc cgaagctttt aagtaatttt tctccgaaac gccatcaaat ttttcatgaa
     8221 tgatgttgga acctaagttg gacgtcatga tgataatcgt gtttttgaaa ttcaccaaac
     8281 gacctttgtt gtcggttaaa cgaccatcgt caagcacttg caacaacacg ttgaacacgt
     8341 cggggtgtgc tttttcaatc tcatccaaca aaatgatgga atatggtttt ctgcgcaccg
     8401 cttcggtcaa ctgaccacct tcatcgtaac caacatatcc cggaggcgct cccactaatc
     8461 tggaaacagc gtgtttttct tggtattcac tcatgtcaat acgcgtcatg gcattttcat
     8521 cgttgaacaa gtattcggcc aatgcttttg ccaattcggt tttaccaacc ccagttgttc
     8581 ctaagaatat aaacgaacct attggtttgc gtttgtcttg caatcctgcg cgtgaacgac
     8641 gaacagcatc cgataaagct tcaatggctt gttcttgtcc caccacacgt ttttccaatt
     8701 cttcttccaa gcgcaacaat tttgccacct cgctttccaa cattttagaa accggaatcc
     8761 ccgtccattt ggctacaact tctgcaattt catcggcatc aacttcttct ttaatcaaat
     8821 tggattttgc ttgaatttca gccaattttt tcttgttttc atccaatttt tgctccactt
     8881 ctttgatttt accgtagcga atttcagcta cttttccgta gtcgcctgca cgttctgctt
     8941 gttgtgcctg catttcaagc gtgtcaattt cttcttttgc ttgttgcaca gcgtcaatca
     9001 cattgcgttc agattgccat tttgcttgaa aagcatcgcg ttgagcgttc aattcggcca
     9061 actctttttc aagtgcctca attttttgaa cgtctttttc acgtttgata gcttctcgtt
     9121 caatttctaa ttgcatgatt ttacgatcaa tctcgtccaa ttcttctggt ttggagttga
     9181 tttccatacg caatttagaa gcagcttcgt caatcaagtc aatggcctta tcgggcaaat
     9241 gacgatcggt aatgtaacgt tgtgacaact caacagctgc aacaatggct gcatctttaa
     9301 tcaaaacctt gtggtgattt tcgtattttt ctttgatacc acgcaaaatt gaaattgcat
     9361 cctcagcact tggttcatcc accaatacgg tttggaaacg acgcaccaag gctttgtctt
     9421 tttcaaagta tttttggtat tcgtttaagg ttgtggcacc cacagcgcgc aattctccac
     9481 gagccaatgc cggtttcaaa atatttgcgg cgtccatagc accttcgcct ccaccacctg
     9541 caccaacaag ggtatgaatt tcgtcgatga ataaaatgat ttcaccatca gaagcaatca
     9601 cttctttcac caccgatttt aaacgttctt caaattcacc tttgtatttt gcacccgcaa
     9661 tcaatgctcc catgtcgagt gaatacacaa ttttgctttt caaattttcg ggtacgtcac
     9721 catttacaat acggtgcgca attccctctg caattgcagt tttacctaca cccggttcac
     9781 caatgagaat agggttgttt tttgttcgac gtgttaatat ttgcaacaca cgtctgattt
     9841 cttcatcacg tccaattaca ggatccaact tgccttgtcg cgccaaatca ttgagatttt
     9901 tagcatattt ttccaacgat ttatagttgc tatcttgcga attggaagtt actttttctc
     9961 catttcttaa atccataatt gcctccttaa aatttttctt agtaatctta ttatccttga
    10021 gcagttgcgc cacattatcc gagccatcca acatggcata aacaatggtt tcaatggtaa
    10081 cgtattcgtc gcccaagttt ttcatttcca caaatgcatt attcaatgtt ttttgtgcat
    10141 tggatgataa atacaattgt ccgccaccgc ttacttttgc tgcactgtct aatattctgt
    10201 ctaaagtttg ttcaacaatg gctgtgttta cattcagttt tgaaaacagg tagggtacca
    10261 cgtccttatc aatattcaaa atacctttca acacatgcgc attttcaatt gcctgatgtc
    10321 ctttttcagt ggcaatcaat tcggcttgtt gtatagcctc ttgcgatttc agtgtaaatt
    10381 tttgaatgtc cattttcttt tcaattttct attggagcgt caatttttaa accaatgcga
    10441 gttttcaagt aaaaatagga aaaaatgaca gatttttatt gatttttgga tgaaaaagcg
    10501 gtgaaatgaa tgactttttg tcggtgttta gtagattttg acgattctca atgtacttgc
    10561 attcaccttt atgtcctctc caactttttt cccaagaagt tgttgtccca gaggtgcttg
    10621 ttgcgataaa cagaatacgg gtgtattttc ataggttact tttccgagcg caacaccaag
    10681 aaaaaaccat cctgttgaga gttgaattaa ggttccgttg gcaatgcaat tggtggttgt
    10741 tgaaaggtgt ttgaagtgat gaatggtttg aagttgttgt tggtattcgg caacttgatg
    10801 ggccaatttt tcaatctcaa gatgcatcat tgctcgaccc gtttcgtgtt tgtctccagc
    10861 cgtacttttg gtgtcggagg cattatcgcg ctgaatgtcg ctgatttctt gttgcaaaaa
    10921 atcaattttc gactgaacaa agtgggttaa gaaatctaaa aattgtgttt tgtccataca
    10981 gctgttggcg gaaaaatacc tgaattaaag tcgcctacaa accagtgctg tgtgcaccac
    11041 ttgtaggcga ccaatattta ttgactgttc actatcagtc ggtatcaatt cattgatcga
    11101 ttaaagtgct cttacagctt ctttaaataa gtttccgcgt tgttcgtagt tttcaaataa
    11161 gtcaaacgat gcacaagctg gagaaagcaa aacagtttcg tctttcttag ccaaacggta
    11221 accataagca actgcttcca ttgccgagcg tgcctctaca atcgtgtcaa caacacctgt
    11281 aaatgcttcg ataattttgg agttatccac acccaagcaa acaatggctt ttacgttggc
    11341 ttttaccaag tccattaatt ctgtgtaatc gtttcctttg tcaacaccac ccacaatcca
    11401 aacggttggt ttatccatac tttccaaggc aaaccaagta gaatttacat tagttgcttt
    11461 tgaatcgttg atgaactcaa ttccgtgaac tttggcaaca aattccaagc ggtgttcaac
    11521 gtttacgaaa tcagatagac tctctctaac tgtttctttt ctaatttcca aaatacgcgc
    11581 tgcaatacca gaagctaacg aattttgtgc attgtgtttg ccttttaggg ctaaatcatg
    11641 aattgacata gtgaattgtt ttttgtttgt tg
//
LOCUS       DAOQVQ010000021        11588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_6285_length_11588_cov_4.049857, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000021 DAOQVQ010000000
VERSION     DAOQVQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_11588_cov_4.049857"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKN22_01330"
     CDS             complement(<1..204)
                     /locus_tag="PKN22_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNS41374.1"
                     /translation="MRVFTPFNVAVMIVFENALSINNQLIELKKSGKKIGFVPTMGAL
                     HSGHISLVNNAKEVCDVVVVSVFV"
     gene            279..1097
                     /locus_tag="PKN22_01335"
     CDS             279..1097
                     /locus_tag="PKN22_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNS41375.1"
                     /translation="MEKKKILFISQEIAPFSPKNEISATARRLPQAIQDAGKEIRVFA
                     PKFGSINERRHQLHEVIRLSGMNIVIDDTDHPLIIKVASIAQARMQVYFIDNEEFFRR
                     KNNTTDENNEEYVDNDERSIFFCRGVLETVKKLGWQPDVIHCHGWMTVPLGLYLKKVY
                     NKDPHFADTKVVYSLYDNKFNKNWDERLAEKLKFDGIDKETIANFKDTDFYSVTASML
                     KYFDGVNIASESLCPKLEEIYTNLDCCKQDFVAEEHQNKVMSEFFDRIIEEALV"
     gene            1135..2412
                     /locus_tag="PKN22_01340"
     CDS             1135..2412
                     /locus_tag="PKN22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNS41376.1"
                     /translation="MVLSATFLLIAFVGINLTSCKKSDNLLGNNYIDPSKLLVSGGVD
                     TFQMETYTVVEDTIYTSGARYGLLGILNDNQFGTMDVGFYSQVRLSGLNPNFGDVPNI
                     TIDSFVLALEYVGGYGYTGNQTFEVFEVDEKMYKDTLYRSTQDLTLKPTNWVQGSGTL
                     SLNPEQLTVVNGDTVNSQLRIPLNPAIALQLIQDSQGNTDFSSNDLFTSNYLKGIYVK
                     TNGLAPGNNQGMVGYFNLLDQDSKLIIYYKENGTAKPPFDLIFSSDCASYNRFKVNNT
                     GKRIESVIANHALGNEEFYAQAGKHRAKINFPTLKNLPKNIVVHEAKLYLPIQALATS
                     KFTYPSTLSITIKGNSTFLPLTATYDSYLKGYVADVRIFVQNYSTGVIPQSELTVSPG
                     TSFISTVDRIVFNGPQTTNKNKPKLIISYTEFK"
     gene            2414..4267
                     /gene="glmS"
                     /locus_tag="PKN22_01345"
     CDS             2414..4267
                     /gene="glmS"
                     /locus_tag="PKN22_01345"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNS41377.1"
                     /translation="MCGIVAYIGKKQAYPIIVNGLKRLEYRGYDSAGIALLNDANDLN
                     IYKIKGKVANLEEFSNGKDISGTIGIGHTRWATHGEPNDVNAHPHYSENEHLALIHNG
                     IIENYDKIKSSLESKGYHFRSETDTEVLVHLIDDIQKNADVSFAESVRLALEQVVGAY
                     AIVVISKDNPNRLIAARKSSPLVVGIGEGGEFFMASDASPIVEHTKNVVYLDDEEIAI
                     LDRFEGLKVYNIRSSEQKSPYIQELELQLEALEKGGYEHFMLKEIHEQPRSIRDCFRG
                     RLSVSEGYVALGGIKDYENKLTNAPRIIMVACGTSWHACLVGEYLIEDLARINVEVEY
                     ASEFRYRNPIINENDIVIAVSQSGETADTLAALKIAKAKGATTLGVCNVVGSSISRET
                     DAGSYTHAGPEIGVASTKAFTAQVTVLTLMALRLAYKKGTISESYYRTLLAEMESIPA
                     KVQSILDSTDQLIKEIAHTYHNATNALYLGRGNSFPVALEGALKLKEISYIHAEGYPA
                     AEMKHGPIALIDEEMPVVVIATQGPSYEKVVSNIQEVKARKGKVIAIVTEGDTQVKAI
                     ADQVIEIPLVDDHFVPLLATIPLQLLSYYIAIMRGCNVDQPRNLAKSVTVE"
     gene            4277..4624
                     /locus_tag="PKN22_01350"
     CDS             4277..4624
                     /locus_tag="PKN22_01350"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNS41378.1"
                     /translation="MNLNERHADFFERVFDIVRLIPKGRVTSYGAIAKALGTPSSSRM
                     VGWAMNASHTHPGVPAHRVVNRNGLLTGKMHFNPPELMEQRLREEGIEVVNDKIIHFD
                     SLFWDPIKELTGE"
     gene            complement(4681..7110)
                     /gene="pheT"
                     /locus_tag="PKN22_01355"
     CDS             complement(4681..7110)
                     /gene="pheT"
                     /locus_tag="PKN22_01355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNS41379.1"
                     /translation="MKVSYNWLKQYTTINATPNDLDKILTDTGLEVEGVEKMEAIKGG
                     LAGVVVGEVMECEKHPDADKLKVTKVNVGTDELLQIVCGAPNVAKGQKVVVATVGTTL
                     YPTPDEPFKIKLSKIRGVESAGMLCAEDELGLGKGHDGILVLDNAIEVGTPAATVFEL
                     EDDYTIEIGLTPNRADAMGHIGVVRDYIAYQNVHHQANLSLTLPALADLTPKSQSEVV
                     KISVENNELCPKYVGITIAGLTVKPSPAWLQKRLRAVGLSPINNIVDVTNYVMRELGT
                     PLHAFDCRKLGGQIVVKTANAGDKFVTLDGVERTLSASNLMITNGKENLCIAGVYGGL
                     DSGVKDDTIAVFIESAYFNPVSVRKTAKEHGLNTDASFRFERGVDPSLTEYALRRCAS
                     LILEVAGGTVAMNETCVGAMETTREVAFNYEHCNRLIGTEIARDKVNEILKNLDITIQ
                     SVNNDDAVLVVPNYRVDVTREADVIEEVLRIYGFNNIPLPEKMNMSVGVFPKPDIEKV
                     QATISEFLVGKGFSETMNNSLTKSAYVEKLGGERLKSENNVPILNPLSNDLDVMRQSL
                     LFNALEVIEHNQNRQNPNLRLFEFGKVYHKYNEYTENKRLLIAVTGKKLAENWNDNAE
                     AHSFYSLKGIVIAVLKRMGLEQFLKEKALENSDLMDGVQLSLLKTKIGEIGMISQKLK
                     KHFDIKNDVFVADLDWDALLNALKMNKIVYKELPKTFAVRRDFSLLLDNAVTFASIEA
                     IAKNAEKNLLKQVGLFDVYEGKNLPEGKKSYAVSFVFQDAEKTLQDAQLDAVMNKIRK
                     QLEEQLGAELR"
     gene            7232..7999
                     /locus_tag="PKN22_01360"
     CDS             7232..7999
                     /locus_tag="PKN22_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS41380.1"
                     /translation="MKKLYKFLLRKLPRPLLIRLSYPFKLIAPLLYKGNNVECPVCER
                     SYSKFLAYGTMNRDNVLCPGDLTLERHRLMWLYLRDFSNFFTAPHLKVLHIAPEQCFH
                     KKFKQQKNLDYLTADLVSPIADMHFDLHKIPLEDNQFDVVFCNHVMEHVDDPIQCMSE
                     LYRVMKPGGWAIMQVPQDIARATTLEDKNITSEADREKYYWQKDHLRLFGMDYPNWLE
                     KVGFHTEIFDKDKNIGKDKMERYRLMEKEIFYIFHKD"
     gene            complement(7996..10215)
                     /locus_tag="PKN22_01365"
     CDS             complement(7996..10215)
                     /locus_tag="PKN22_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS41381.1"
                     /translation="MSHLPNLIIDLTIILTAGGVVTLLFKKLKQPLVLGYILAGFLVS
                     PNFHHFPTITDIKNIEIWSQIGVIFLLFSLGLEFSFKKLTQVGGTASVTALVEITGIG
                     FVGFMVGTLLGWNTMDSLFLGGMLSLSSTIIIIRTFDELGIKTEKFVGVVLGTLIVED
                     LLAILLMVLLSTIATGSGSAGDASILWSVLKLVFFLVLWFVSGIFFIPTILKKTKKLM
                     NDETLLVVSIALCFIMVIFAVNAGFSKELGAFIMGSILAETKQREKIEHLVSPLKDLF
                     GAIFFVSVGMLIDPNMIVEYIVPIVILVFVILFINSLITSTGAFLSGQPLKTSIQTGM
                     SMAQIGEFSFIIATLGLSLGVISDFLYPIAVTVSAITTLTTPYMIKASPKVISWVERK
                     LPERVMTRLNNYTINAQSANATKDWHQLVKVSITNSLIYSVILITIIFAFSRYLAPIF
                     FEQFGENLTTRIIASLITLVAMSPFLWAIIARNFKSELRSRLLAQEKYRALVYMVSLF
                     KVALAVFFISFMLRAYFSNTVTVSSVIIILFFLVVFRKKVQRFYNYFEDRFKTNLNVK
                     DNQAENVAKMAPWDSHIVVIKVPANSPIIGKKLSELQWREKVGVNVASIMRGEHCIRI
                     PKANEIIYPYDELGLIGTDEQLAEMELIIVPRSLHSSKIQEAPNVILHALTVRKDSPN
                     IGKTIRSSGLKEKTHGLVVGIERDGERYLNPESNWMLMPGDIVWIVGDPNLIQKYVD"
     gene            complement(10567..11436)
                     /locus_tag="PKN22_01370"
     CDS             complement(10567..11436)
                     /locus_tag="PKN22_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNS41382.1"
                     /translation="MITYNPKSWLKLVFSFRKSDTTRILLIPIISMAVFTTLIAYIEI
                     NYLKDVAYLKNLMTIYTLIGFVISMLLVFRTNTAYDRWWEGRRKWGELINESRNIAIK
                     LSHLIKDKDEHNFYNRMLPNFAFALKTHLQNGVNNWSELDLTAEELQELKSKEHVPAH
                     IVKMIYQKIYELKAIQQISEIDFLVLDKQLKTLMEICGACERIKKTPIPYSYSMFLKK
                     FILIYVVTIPIALVPLLGYVASAIATFIFYILMSIEIIAEEIEDPFGGDDNDLPTDVI
                     AMNIKKSVHELLN"
BASE COUNT         3802 a         2127 c         2054 g         3605 t
ORIGIN      
        1 cacaaaaaca ctcaccacca ccacatcaca tacctctttt gcgttattta caagagaaat
       61 atgccctgaa tgcaaggcgc ccattgtggg tacaaatccg atcttttttc ctgatttttt
      121 taattcaatt aactgattat taattgatag cgcattttca aatactatca ttactgcgac
      181 attgaaagga gtaaaaacac gcaaaactat ttttttttct tgaaaatcag agaattttta
      241 tatatttttg taaagttttt tcaaacctat aaacacaaat ggaaaaaaag aaaattctat
      301 ttatatcaca agaaattgca cctttttcgc caaagaatga aatttcagct actgccagaa
      361 gacttccaca agcaattcaa gatgcaggta aggaaatacg cgtttttgca cccaaatttg
      421 gaagcatcaa tgaaagaaga caccaattac acgaagtaat tcgcctttcg gggatgaata
      481 ttgtaattga tgataccgat cacccactta tcattaaagt tgcttccatt gcacaagcac
      541 gcatgcaagt ttattttatt gataacgagg agttttttag acgaaaaaac aacacaaccg
      601 acgaaaataa tgaagaatat gtggacaacg atgaacgttc cattttcttt tgtcgcggag
      661 tattggaaac tgttaaaaaa ttaggctggc aacccgatgt aatccattgc catggttgga
      721 tgacggtgcc tttgggcttg tatttgaaaa aagtatataa caaagacccg cattttgctg
      781 ataccaaagt tgtatatagt ttatacgaca ataagtttaa caaaaattgg gacgaaagat
      841 tggctgaaaa acttaaattt gacggcatcg ataaggaaac aattgcaaac tttaaagata
      901 ctgactttta ttcagttact gcaagtatgt tgaaatattt tgatggcgtg aacattgcat
      961 cagaatcatt gtgtccaaaa ctcgaagaaa tttacaccaa tttggattgt tgtaaacaag
     1021 attttgtagc agaagaacat caaaacaaag tgatgtctga attttttgac cgtataatag
     1081 aagaagcact tgtataaaaa aaataaaaat agttgtaaaa gttggcggag agaagtggtt
     1141 ctttccgcta cttttttatt gattgcattt gttggaatca atttaacgag ttgtaagaaa
     1201 agtgataatt tattgggaaa caactacatc gatcctagta aattgttggt ttctggcggt
     1261 gttgatacgt ttcaaatgga aacctacacg gttgtagaag acaccattta caccagtggc
     1321 gcacgctatg gattgcttgg tattttgaac gacaaccaat ttgggacaat ggatgttgga
     1381 ttttactctc aagtgcgttt gtctggtttg aatcctaatt ttggtgatgt accaaacatt
     1441 accatcgatt cttttgtttt ggcattggaa tatgtagggg gctatggcta cacaggcaat
     1501 caaacctttg aagtatttga agtggacgaa aaaatgtaca aagatacctt gtaccgttcc
     1561 acacaagatt taaccctaaa accaacaaac tgggtacaag gaagcggaac actgagttta
     1621 aatccagagc aactaactgt tgttaatggc gatactgtta attctcaatt gcgcattcct
     1681 ttgaatcctg ccattgcttt gcagttgatt caagattcac aaggcaatac tgattttagc
     1741 agcaacgatt tgtttacttc caattatttg aaaggaattt atgtaaaaac aaatggtttg
     1801 gctccaggaa ataatcaagg aatggttgga tattttaatt tgttggatca agattctaaa
     1861 ttaataattt attacaaaga aaacggaact gccaaaccac catttgattt aattttctca
     1921 agtgattgtg caagttataa ccgtttcaaa gtaaacaata caggcaaacg tattgaaagc
     1981 gtaattgcga atcatgccct tggtaacgaa gaattttatg cacaagctgg taagcaccga
     2041 gcaaaaatta attttccaac gttgaaaaat ttaccaaaaa atattgtggt gcacgaagca
     2101 aaattgtatt tgccaattca agcattagca acttctaaat ttacctatcc atcaacatta
     2161 agcatcacca taaaaggaaa cagcacattc ttgcctttaa ctgcaacgta tgatagttat
     2221 ttaaaaggct atgtagcaga tgttcgaatt tttgtgcaga attattcaac aggagttatt
     2281 ccacaaagtg aattgacagt ttcgccagga acttctttta tatccactgt ggatagaatt
     2341 gtgttcaacg gacctcaaac aaccaataaa aataaaccta aattaatcat ctcttatact
     2401 gaatttaagt aatatgtgtg gaattgtagc gtatatcgga aaaaaacaag cgtatccaat
     2461 tattgttaat ggattaaaac gcttagaata tcgtgggtat gacagtgctg gtattgcatt
     2521 attaaatgat gccaacgatt tgaatattta caaaattaaa ggaaaagttg cgaatttgga
     2581 agagttttca aatggcaaag atatatctgg tacaatagga attgggcaca cacgttgggc
     2641 tactcatgga gaaccaaacg acgtgaacgc acatcctcat tattctgaaa acgaacattt
     2701 agcgttgatt cacaatggaa ttattgagaa ttacgacaaa attaaaagca gcctagaaag
     2761 caaaggatac cattttagaa gtgaaaccga tacagaagtg ttggtgcatt taattgatga
     2821 tatccaaaaa aatgccgatg tttcatttgc agaatcagtg cgtttagcat tggaacaagt
     2881 ggtaggagct tatgcaatag ttgtgatttc aaaagacaat cccaaccgtt taattgctgc
     2941 aagaaaaagt agtccacttg ttgttggaat tggtgaaggc ggtgagtttt tcatggcttc
     3001 ggatgcatcg ccaattgtag agcacacaaa aaatgtggtt tacttagacg atgaagaaat
     3061 tgctattttg gaccgttttg aaggcttgaa agtgtacaac attcgtagta gtgagcaaaa
     3121 aagtccatac atccaagaat tggaattgca attagaagca ttggaaaaag gtggctatga
     3181 acactttatg ctcaaagaaa ttcacgagca acctcgttct atccgcgatt gcttccgtgg
     3241 acgtttaagc gtttctgaag gatatgttgc tttgggcgga attaaagatt acgaaaataa
     3301 attaaccaat gcaccacgca tcatcatggt ggcttgtggt acttcttggc acgcatgctt
     3361 ggttggtgaa tatttaatag aagatttggc acgtatcaac gtggaagtgg aatatgccag
     3421 tgaatttaga taccgtaacc ctatcatcaa tgaaaacgat attgtgattg ctgtatctca
     3481 atcgggtgaa actgccgata ccttggctgc attgaaaatt gctaaagcaa aaggcgcaac
     3541 aacattgggt gtttgcaacg tggtaggttc atctatctca cgcgaaacag atgcaggttc
     3601 atacacccac gctggaccag aaattggggt ggcttccaca aaagcattta cagcacaagt
     3661 tacagtgttg accttaatgg cattgcgttt agcctataaa aaaggaacta tcagtgaatc
     3721 gtattataga accttgttgg cagaaatgga gtctattcct gctaaagttc aatccattct
     3781 tgattcaacc gaccaattaa tcaaagaaat tgcacatact tatcacaatg caacaaatgc
     3841 gttgtatttg ggtagaggaa acagtttccc agtggcgttg gaaggtgctt tgaaattgaa
     3901 agaaatttct tacatccatg ctgaaggata tccggctgct gagatgaaac acggaccaat
     3961 tgccttgatt gatgaagaaa tgccagtggt ggtaattgct actcaaggtc catcttatga
     4021 aaaagtggtt tccaacattc aagaggtgaa agctcgtaaa ggaaaagtga ttgctattgt
     4081 tacagaaggt gatacgcaag tgaaagccat tgctgatcaa gtgattgaaa ttccgttggt
     4141 ggacgatcat tttgtgccgt tgttggcgac tattccactg cagttgttgt cgtattacat
     4201 tgccatcatg cgtggatgta atgtggatca accacgaaac ttggcaaaat cggttacggt
     4261 agaataatag cttggcatga acctcaacga acgtcatgcg gatttctttg agcgtgtttt
     4321 tgacatcgta cgtttaattc ccaaaggaag agttacgtca tacggagcaa ttgcaaaagc
     4381 attgggcaca ccttcatcat caagaatggt gggctgggct atgaatgcct cgcatacaca
     4441 cccgggagtt ccagcgcatc gtgtagtaaa cagaaacggc ttgttgacag gaaaaatgca
     4501 cttcaatccg ccagaattga tggaacaacg tttacgtgaa gaaggaattg aagtggtaaa
     4561 cgataaaatc attcattttg actccttatt ttgggatcct ataaaagaat tgacaggaga
     4621 ataaataaaa aatgcgttga ccaatcatca acgcattttt ttgtgttata gatttttgtt
     4681 ttatcttaat tctgctccta attgctcttc caattgcttg cgaattttat tcataactgc
     4741 atccaattgt gcatcttgca aggttttttc tgcatcttgg aatacaaacg aaacagcata
     4801 ggattttttg ccttctggta agtttttgcc ttcatacaca tcaaacaagc ccacttgctt
     4861 caataagttt ttctccgcat ttttagctat tgcttcaatt gatgcaaaag ttacagcgtt
     4921 atccaatagc aatgagaaat cacggcgaac agcaaacgtt tttggtaatt ctttgtatac
     4981 tattttattc attttcaacg cattcaaaag cgcatcccaa tccaaatcgg ctacaaaaac
     5041 atcgttttta atatcaaaat gttttttcaa tttttgggaa atcattccaa tttcaccaat
     5101 ttttgttttc aacaaactca attgaacacc atccattaaa tcagaatttt caagcgcttt
     5161 ttcttttaaa aactgttcta atcccatgcg tttcaataca gcaattacaa ttcctttcaa
     5221 actataaaag ctgtgcgctt cggcattatc gttccagttt tctgccagtt ttttacccgt
     5281 tacagcaatc aataaacgtt tgttttcagt gtattcgttg tatttgtgat agacttttcc
     5341 aaattcaaat aagcgcaaat taggattttg acggttttga ttgtgttcga taacttccaa
     5401 tgcattgaac aataacgatt gacgcatcac atccaaatca tttgacaagg ggttgagaat
     5461 tggaacgttg ttttctgatt ttaaacgctc accccccaat ttctccacat acgccgattt
     5521 ggtgagtgag ttattcatcg tttcactgaa cccttttcct accaaaaatt cgctaattgt
     5581 tgcttgcact ttttctatat ctggtttagg gaaaacgccc accgacatgt tcattttttc
     5641 gggtaatgga atgttgttga aaccataaat acgcaatact tcttcaatca catcggcctc
     5701 tcgggtaaca tccacacggt agttaggtac cactaaaaca gcatcatcgt tgttcaccga
     5761 ttgaatggta atatcgagat tttttaatat ttcattgact ttatcacggg ctatttccgt
     5821 cccaattaaa cggttgcaat gctcataatt aaacgccact tcgcgtgtag tttccatcgc
     5881 tccaacgcag gtttcattca ttgcaacggt tccaccagct acttccaaaa tcaacgaagc
     5941 acatctgcgc aacgcatatt cggtgaggga aggatctaca ccacgctcaa aacgaaacga
     6001 tgcatcggtg tttaagccat gttctttggc tgtttttctt acagaaacag gattaaaata
     6061 agccgattct ataaaaacag caattgtatc atctttaact ccagaatcca aaccaccata
     6121 cactccggca atgcacagat tttctttgcc gttggtaatc attaaattag aagcagaaag
     6181 agtgcgttct acaccgtcca aggttacaaa tttatctcct gcatttgctg tttttactac
     6241 aatttgaccg cctaatttac ggcaatcaaa tgcatgcaaa ggagttccca attcacgcat
     6301 cacatagttg gtaacatcca caatattgtt aattggcgat aaaccaacag ctctcaaacg
     6361 tttttgcaac catgcaggtg aaggttttac ggttaaacca gcaatggtaa ttcccacata
     6421 tttagggcac aattcgttat tttctaccga aatttttaca acttctgatt ggctttttgg
     6481 tgttaaatca gccaatgctg gcaaggtcaa cgataaattt gcttgatggt gcacattttg
     6541 gtaagcaatg taatcgcgca caacgcctat gtgtcccatt gcatctgcac ggttaggtgt
     6601 taacccaatt tctatggtat aatcgtcttc caactcaaaa acagttgctg ctggcgttcc
     6661 aacctctatg gcgttatcca acactaaaat tccatcgtgt cctttaccta atcccagttc
     6721 atcttcggca cacaacatac cggcagattc tacccctcta attttagata atttaatttt
     6781 gaacggttca tcaggagtgg gatacaaggt agttcccact gttgcaacaa ccactttctg
     6841 acctttagcc acattgggag ctccacaaac aatttgcaac aattcgtcgg taccaacatt
     6901 tactttggtt actttcaatt tatcggcatc aggatgtttt tcacattcca tcacttcacc
     6961 aactaccaca cctgccaaac cacctttgat ggcttccatt ttctcaactc cttcaacttc
     7021 taaacccgtg tcggttagaa ttttgtccaa atcgtttgga gtggcattaa ttgttgtgta
     7081 ttgtttcaac caattgtaag atactttcat tgttgttcaa atgttgtgtt tatatttgtt
     7141 caaagctaca aaattacgat aaattggcaa ttagaaaaca gataagacta taagtcgcat
     7201 tttaacttta tatttgtgca aaatcacgaa aatgaaaaaa ctatataaat ttttgcttcg
     7261 caagttacca cgtccgttat taattcgttt gagctatccg ttcaaattga ttgctccctt
     7321 attatacaaa ggaaataatg tagaatgtcc ggtttgcgaa agaagttatt ctaagttttt
     7381 agcttacggc actatgaatc gtgataacgt attgtgtccc ggtgatttaa cgttggagcg
     7441 ccaccgttta atgtggttgt atttacgcga tttttccaac ttttttacag cgccgcattt
     7501 aaaagtattg cacattgcgc cagaacaatg ttttcataaa aaattcaagc aacaaaaaaa
     7561 cttggattat ttaaccgccg atttagtgtc gcccattgct gatatgcatt ttgacttgca
     7621 taaaattccg ttagaagaca atcaatttga tgttgtgttt tgcaatcatg tgatggaaca
     7681 tgttgacgat ccgattcaat gcatgagtga attgtatcgc gttatgaaac caggaggatg
     7741 ggctatcatg caagtgcctc aagatattgc aagagcaaca actttagagg ataaaaacat
     7801 cacttcagaa gccgatagag aaaaatacta ttggcaaaaa gaccatttgc gcttgtttgg
     7861 tatggattat cccaactggt tagaaaaagt ggggttccac accgaaattt tcgacaagga
     7921 taaaaatatt ggcaaagaca aaatggaacg ttaccgatta atggaaaaag agattttcta
     7981 tattttccac aaagattaat cgacgtattt ctgaattaaa ttaggatcac ctacaatcca
     8041 aacaatatct cctggcatca acatccaatt ggattctgga ttaagataac gttctccatc
     8101 acgttcaata cccacaacca atccgtgtgt tttttctttt aacccagaac tgcgaatagt
     8161 ttttccaata ttgggtgaat cttttctgac tgttagtgca tgcaaaatca catttggtgc
     8221 ttcttgtatt tttgagctat gcaaagatcg tggaacaatg attaattcca tttctgccaa
     8281 ttgctcatcc gttccaatta accctaattc atcatacgga tagataattt cattagcttt
     8341 tggaatgcga atacaatgct cgccacgcat gatcgaagca acgttgacac ctactttttc
     8401 acgccattgc aactcgctca atttttttcc aattataggt gaattagcag gtactttaat
     8461 caccacaatg tgagaatccc acggagccat ttttgctaca ttttctgcct gattgtcttt
     8521 tacattcaaa ttggttttaa aacggtcttc gaaataatta tagaatcgtt gtactttttt
     8581 tctaaaaaca accaagaaaa ataagataat gataacgctg gaaacagtga cagtatttga
     8641 aaaataggcg cgcaacataa atgaaataaa gaacacagcc aacgcaactt taaacaagct
     8701 aaccatgtaa acgagcgcac ggtatttttc ttgagccaac aatctgcttc ttaattctga
     8761 tttaaaattt cttgcaatta ttgcccataa aaaaggtgac attgcaacca aagtaatcaa
     8821 agaagcaatg attcttgttg ttaaattttc tccaaattgt tcaaagaata taggtgccaa
     8881 ataacgcgaa aaggcaaaaa taatggtgat taaaataacc gaataaatca aagaattggt
     8941 aatagacact ttaaccaatt gatgccaatc ttttgttgca tttgccgatt gtgcattgat
     9001 ggtgtaattg ttcaatcgtg tcattacacg ctcaggcaac ttgcgttcta cccaacttat
     9061 aacttttggt gaggctttaa tcatgtaggg tgttgtcagc gttgtaatgg ctgaaacggt
     9121 tacagcaatt ggataaagaa aatcactgat gacaccaagt gacaaaccta aagtggcaat
     9181 aatgaacgaa aactcaccaa tttgcgccat actcatccct gtttgaatgg atgtttttag
     9241 tggttgtcca gataaaaatg ctcctgttga tgttattaaa ctattgataa ataaaataac
     9301 aaaaacgaga ataacaatag ggacgatgta ttcaacaatc atgttggggt caatcaacat
     9361 cccaaccgat acaaagaata ttgctccaaa taaatctttt agtggactca ctaagtgttc
     9421 aattttttca cgctgcttgg tctctgccaa aatagaaccc atgataaaag cacccaattc
     9481 ttttgaaaaa ccagcattta cagcaaaaat caccatgatg aaacaaagag cgattgacac
     9541 gaccaacaat gtttcatcat tcattaattt ttttgttttc ttaagtattg tgggaataaa
     9601 aaagataccc gaaacaaacc acaaaactaa aaagaatacc aattttagta ccgaccaaag
     9661 aatggatgca tctcccgctg agcctgatcc tgtggcgatg gtagaaagta ataccattaa
     9721 taaaattgca agtaagtctt ctacaatgag tgttccgagg actacaccaa caaatttttc
     9781 agtttttatt ccgagttcat caaaagtccg aataataata atggttgagg ataaagataa
     9841 cattccgccg aggaataagc tatccatggt attccatccc aaaagggttc ccaccataaa
     9901 accaacaaag ccgattccag taatctcaac caatgccgtg actgaagctg tgcctcccac
     9961 ttgcgttaat ttcttgaaac taaattctaa tcctaaacta aacaagagaa aaataacacc
    10021 aatttgcgac caaatttcaa tgtttttaat atcagtaatg gttgggaagt ggtgaaaatt
    10081 gggactcacc agaaaacctg caaggatata accgagtact aagggttgtt ttaatttttt
    10141 aaataaaagc gtaactacac ctccagcagt tagtatgatt gttaaatcaa tgattaagtt
    10201 gggtaaatga ctcacttata tgtaattttt acaaaaatag taaaaaaaaa taaaatgcga
    10261 aaggggaaga cttacgtcct ccccttcaca ttaacctaaa ctactactac ttatgaaaac
    10321 taactcttac tactactaat ttaacctatt caaacattgt gccaaacaca ttttgttttt
    10381 tcggtggatt atcatttctg atatcttatt acaaatttaa gctatttcat cgaatattcc
    10441 aaaaaaaact gtttaaaaat taataggcat tcttgaaaag ctaacagaaa taaatgaact
    10501 tttatacaaa aacgataaat tgtatggaat attgtgtaaa gagtaaatta aattattgtt
    10561 tttcaattaa tttaaaagtt catgtacact ttttttgatg ttcattgcaa tcacatcggt
    10621 tggtaagtcg ttatcatcac ctccaaatgg atcttcaatt tcttctgcaa tgatttcaat
    10681 actcataaga atgtaaaaga taaaagtggc tattgcagag gctacatacc ctaaaagagg
    10741 caccaatgca attggaatgg taacaacata aattaagatg aattttttca agaacatgct
    10801 ataagaatag gggatgggtg tttttttaat acgttcacac gccccacaaa tttccatcaa
    10861 cgtttttaat tgtttgtcta aaaccaaaaa gtcaatttca gaaatttgtt gtattgcttt
    10921 taattcgtat attttttggt aaatcatttt tacaatatgc gcgggcacgt gttcttttga
    10981 tttcaattcc tgcaattctt cggctgttag atctaattcc gaccaattat tgacaccatt
    11041 ttgtagatgc gtttttaatg caaaagcaaa attgggcaac atgcgattgt agaaattgtg
    11101 ttcatctttg tcttttatta agtgtgataa ttttattgca atattgcgtg actcgttgat
    11161 taattcgccc cattttcgtc gaccttccca ccatcggtca taagctgtat tggttctaaa
    11221 aactaaaagc atcgaaataa caaaacctat taaagtgtat attgtcatta ggttttttaa
    11281 ataggcaacg tctttcaaat agtttatttc tatataagca attagcgttg tgaaaactgc
    11341 catggaaata attggaataa gcaaaatgcg tgtagtatcg ctttttcgaa aggaaaaaac
    11401 tagtttcaac caactttttg gattgtatgt aatcataaat tgttttttgc aaaaatattt
    11461 ttcttcgctt gaaagaaaaa atacttaaca aaaattatgc ttcatccaca cgtacaaaaa
    11521 ttataaaagt tcttcatttg gttctaacaa caaaaactat tgtattttca aaaaagtaga
    11581 ctgaatac
//
LOCUS       DAOQVQ010000022        11587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_6286_length_11587_cov_3.843306, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000022 DAOQVQ010000000
VERSION     DAOQVQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6286_length_11587_cov_3.843306"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PKN22_01375"
     CDS             <1..1050
                     /locus_tag="PKN22_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNS41383.1"
                     /translation="VSIASDIVPIMGENRILAHHGMRLLNEHPRVAFKKLLLLGKRSF
                     PVVLEDVVFTIAPRINAAGRIYSGRKAVELMISNDEVEIGKLAQEIEVYNQERKSIEA
                     ATTQEALSIIEKNEWYHTAKSTVVHQEGWHKGIVGIVASKLIDKHFKPTIVLTQLDDK
                     LTGSARSVNNFDIYKAIAACEDLLEQYGGHTHAAGLTLKKENLAQFRTKFEEIVREKL
                     TLEDALPVEYIDLELNFDAIFTSSENRTLVPRMKRILKQMEPHGPHNMKPVFYSSNVY
                     SKSIKILKDEHLKLQVTQPDCDVVLDAIGFFMADKQLDVAAGMPFEMLYTLETNIWNG
                     KESLQLMIKDIRPLA"
     gene            complement(1085..1453)
                     /locus_tag="PKN22_01380"
     CDS             complement(1085..1453)
                     /locus_tag="PKN22_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41384.1"
                     /translation="MAKRRAIVSYEKLTVEQKKRLENDFPDGFYGKLTPIKTPTGETL
                     DALIWETEDIIYLVKINKAAYSASLDDDDDDDFVDDTLGGDIPVDGDDKDDDIDDDVD
                     DDDDDDDDDDDDDDDDDDEE"
     gene            complement(1459..1743)
                     /locus_tag="PKN22_01385"
     CDS             complement(1459..1743)
                     /locus_tag="PKN22_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41385.1"
                     /translation="MDSEFKLVEKEEVVNFHFPKEDVQLSDSEKKALKAQLERANTLG
                     NAEHHKVKIFFIDEEAKKVVYTTIWGVTENHILLKQNVFLPIQRIVKLEI"
     gene            complement(1885..3252)
                     /locus_tag="PKN22_01390"
     CDS             complement(1885..3252)
                     /locus_tag="PKN22_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNS41386.1"
                     /translation="MSSFATMISNQRLFFHSNATKSVDYRIKQLKTLQKTLQANEEVI
                     NKALFADFKKSDYETYTTELALVYGDIKEAIKKIRKWSKPKKVRTNLANFPAKSYILP
                     EPLGVSLIIGAWNYPIQLTLAPMVAAMAAGCTVVLKPSEVASNTSGVLAQIIAHAFDE
                     NYIAVVEGDASITTALLEERFDKIFFTGSTHVGRIVYQAAAKNLTPVTLELGGKSPAI
                     VTPSCSIEMTAKRLVWAKFLNAGQTCIAPDYVVVHKTVEKQLLEAVKKEIQKAHYSIE
                     NKNFVQIISKKHVERLLQLMAPSTVYFGGEYNKEERFIEPTLLVDVDFTDKVMQEEIF
                     GPILPFIAYTHLNEVIHTIKQLEKPLSCYVFTNDKQEKEKILNELSFGGGAVNDALMH
                     ISNSNLPFGGVGNSGMGAYHGKAGFDAFSHFKSIIDKPTWMELSIKYSPYSMKKLGLI
                     KRLLG"
     gene            3336..5042
                     /locus_tag="PKN22_01395"
     CDS             3336..5042
                     /locus_tag="PKN22_01395"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41387.1"
                     /translation="MNYQFKIEKPNQQYIQITAETVALTDNPVIQLPSWRPGRYELGN
                     FAKNVKGFKVFNGNGKPVEFLKISKDAWQLTLAKGETIKIQYAYYAHLLNAGSTFFDA
                     TQLYVNPVNCCVYQVGEEQAPCTAELAIPEHWQIAGSMQQEGRKLQAANFDELADSPF
                     ICSANLQHDTYEVGATTFHVWFNGITKPDWERVLRDFKAFSAKQIEKFSEFPVDEYHF
                     LIHILPTKAYHGVEHCKSTVICLGPSYDVFGSLYKELLGVSSHELYHTWNVKSIRPIE
                     MFPYDFTKENYSRLGYLCEGVTTYMGDLFLLKSKVFSVEQYLVELAARFRIHFDNHGR
                     FNYSVAASSYDTWLDGYEPGAPNRKVSIYTEGSLLAFVADITLLKNTNNKYGLDEVMR
                     RLFFNYALQGKGVSEQDYKNELEAVGNCSFDDYFQKFVWGNEPYEAILTDALEYIGLE
                     LVQKPAAAYSQARLGVKCIVDGDKTVAKNFYPGAPADMGGMMVDDQILAVNGVVINGN
                     FDQWLNYFDEDEKRITVIRAGKLVELVLPEVSRNFYLEHQLKCVENPNNFQKKGFAVW
                     SE"
     gene            5073..6755
                     /locus_tag="PKN22_01400"
     CDS             5073..6755
                     /locus_tag="PKN22_01400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNS41388.1"
                     /translation="MNDNCSKFIAFQKDVSAIALPQKFTFPFYYEPHPLAVIASEELQ
                     AYINKQEGWQHNFGLNPAMTGMVIGKMFGVLVVQNQQNELGYLAAFSGKLADKNDYEH
                     FVPPVFDMLTEGSFYRKGERELLEQSAVIEQLLQSDELKLLKNEVVLVEQTCQQEIID
                     FKEKAKISKENRKIQRENAELLSEEEKIRLFKELDRQSIHENFALKDLKKIHLEKKQA
                     AQANLDDFLNRIKTLKEKRKQQSQALQNQLFDQYHFLNARNETRSVLSIFEHIENGKP
                     PSGAGECCAPKLLHYAYQHQLKPIALAEFWWGASPASEIRKHQQFYPSCRGKCEPILG
                     HMLQGLDVDPNPMKINPAEGRELEIAYEDEDIVVVNKPTEFLSVPGKTIQDSVLKRME
                     TYLVGASGPLLIHRLDMSTSGILLVAKNKFAHDFLQRQFIKRSVKKRYVALLEGELKE
                     HEGFIDLPLRVDLDDRPRQLVCYEYGKQAQTKWELIEVKNGKSKVYFYPLTGRTHQLR
                     VHAAHPLGLNMPIVGDDLYGTKANRLHLHAEQLTFRHPRTKELMTVHVPAEF"
     gene            6795..7469
                     /gene="trmD"
                     /locus_tag="PKN22_01405"
     CDS             6795..7469
                     /gene="trmD"
                     /locus_tag="PKN22_01405"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNS41389.1"
                     /translation="MRIDILTILPELLAGPFSASIMKRAQEKGHAEIHLHNIRDYSTN
                     KHKNVDDYQYGGGAGMVMQIEPIAAVIEKLKSERHYDEIIYMTPDGEVLDQAICNQFS
                     AHENLMILCGHYKGVDERVREHYITKEISIGSYVLSGGELAAAVLVDSVVRLIPGVLN
                     DETSALTDSFQDNLLSPPVYTRPPEFNGWKVPDVLLSGDFKKIDKWREEQALERTKQR
                     RPDLLK"
     gene            complement(7472..8773)
                     /locus_tag="PKN22_01410"
     CDS             complement(7472..8773)
                     /locus_tag="PKN22_01410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS41390.1"
                     /translation="MNEKVEQLRKLGHKELTKIQQLVFQSNEPYNELVLYAPTGSGKT
                     LTFLIAILEHLKNEASNSPQLLVLSPTRELALQIETVFKSLKTNFSVTTCYGGHAIKT
                     EKNNLAARPQVIIGTPGRITDLMHRKLIDFSFTPNLIIDEFDKCLELGFLSDIEQIYK
                     QLRQLKKSIYCSATKLEQFPPIIRFTQPLTFDVTTTQNKQQTFYQVEASKGKLETLVQ
                     LIIDFQLQPTILFCNFREDVDALKNYFDDLEIATTAFHGGMEQDERERSLIKFKNGSA
                     PVLICTDLGARGIDIAGVQHIVHYQLPQDEASFTHRNGRTARMHETGAIYFFENDAPT
                     VNYSLPEAKKYALTNKKSYQAPNWLTIYFSGGKKDKINKIDLVGFICQKGNLPKDKVG
                     MISVMDFQSYVAIDRKSCGKLLPELRKHKIKGQKVRIAISK"
     gene            9076..10284
                     /locus_tag="PKN22_01415"
     CDS             9076..10284
                     /locus_tag="PKN22_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNS41391.1"
                     /translation="MVVLFLLFFSLVAYSQAPAIDYNNKLLWEISSKGSKQKSYLFGS
                     YHTNDKRVFDLSDSVYFALNQAKAIVLETDVYAMFKDWDTRRDDFRLNYDNQGNPYTN
                     SNNSSRTAYGDEDGMPQFLDLYFLQYGFNAQKEFYVLEPIEDQMNLTENTSNYLYPYI
                     NDYTSFGEKSIKLYTEGNIDAIEKLMQTGMRWNPEFYDKLILKRNVVMANGIDSLIKN
                     GPLFISIGAGHLSGNKGVINLLRSKGYQMRPVLWSKSATATQAKVQVQSFKSYQYIDT
                     ITGLNVTLPGKPFEIEKEGVVLDLIYREMGQGNTYSVEVIKRQEGQTLETVAHDYILS
                     PGDAKMKQVIVDNEFSYYEGISDSYPEGLAWTRVIETYDYFAFIKAYGGNKFMNSKRP
                     QKFFDNVWFE"
     gene            complement(10287..11360)
                     /locus_tag="PKN22_01420"
     CDS             complement(10287..11360)
                     /locus_tag="PKN22_01420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS41392.1"
                     /translation="MQKKKVFIFCPYPVDTAPSQRFRYEQYINLLEQQNFEFHFFPFI
                     DTKTWNVLYQKGRYFLKTYLMLMSFFKRWVVLFKIKEADVVFIHREMAHFGPPIFEWF
                     ISKVLKVNYIYDFDDAIWLPNYSKSNAKVQWIKCYWKVPYCIKWAHKVSVGNQFLADY
                     AQPYNSNVVIIPTTLDTENYHNQTIDYAHQRLSIGWTGTLTTMNYLYELEPVIAQLEK
                     EFSFDFIVISNQKPKMELQSLRYIQWNKDTEIADLMKFSIGVMPLKEDKWSKGKCGFK
                     ALQYMSLGIPCVISPVGVNTAIVDHQTNGFLATSNDEFYSYLKQLLENATLRKEIGLK
                     GQQTVINRYSTSANLKKYVELLQ"
BASE COUNT         3734 a         2074 c         2132 g         3647 t
ORIGIN      
        1 gtgagtattg caagcgatat cgtgccaatc atgggtgaaa atagaatttt agcccatcac
       61 ggaatgcgct tattgaacga acatccacgt gttgctttta aaaaattatt actgctgggc
      121 aagcgtagtt ttccagttgt actggaggat gttgttttta ccattgctcc ccgtatcaac
      181 gcagcaggga gaatttattc gggcagaaaa gctgttgaat tgatgatttc taatgatgaa
      241 gttgaaattg gtaagttggc acaagaaatt gaagtgtata atcaagaacg aaaatccata
      301 gaagcagcaa ccacgcaaga agctctttca ataattgaaa agaatgagtg gtatcataca
      361 gccaagtcaa ctgtcgtgca tcaagaagga tggcacaaag gaattgtggg aattgttgct
      421 tccaaattga tagacaaaca ttttaaaccc actatagttc tcacacaatt ggacgataaa
      481 ttaacaggtt cggcgcgatc ggtcaataat tttgacatct acaaagccat tgcagcatgt
      541 gaagatttat tggaacaata tggagggcac acccacgcag caggtttaac gctcaaaaaa
      601 gaaaatttgg ctcaatttag aactaaattt gaagaaattg ttcgagaaaa attgacgtta
      661 gaagacgctc tacctgttga atacattgac ctagagttga attttgatgc tattttcaca
      721 agttcagaaa atagaacttt agttccacgc atgaaacgca tcctaaaaca aatggaacca
      781 catggtccgc acaacatgaa acctgtcttt tactcatcca atgtttactc aaaatcaatt
      841 aaaattttga aagacgaaca tttaaaatta caagtaaccc aaccagattg tgatgttgtt
      901 ttagatgcca ttggtttttt tatggctgat aaacaattgg atgttgcagc aggaatgcct
      961 tttgaaatgc tttacacttt ggaaactaac atttggaatg gaaaagagag tttgcagtta
     1021 atgataaaag atattcgccc actagcttaa acaaaaaaaa gccgaattac ttcggcttta
     1081 attattattc ttcatcgtcg tcatcatcgt cgtcgtcgtc gtcatcgtcg tcgtcatcgt
     1141 cgtcgtcatc aacatcatcg tcgatatcat catctttgtc gtcaccgtca actggaatat
     1201 ctccacccaa agtatcatca acgaaatcat catcatcatc atcatccaaa gaagcagaat
     1261 aagcagcttt attgattttt actaaataaa tgatgtcttc tgtttcccag attaaagcat
     1321 ccaatgtttc accggtaggt gtttttatag gagttaactt tccataaaaa ccatctggga
     1381 aatcattctc caatcttttc ttttgttcca cagttaactt ttcatagcta acaatcgctc
     1441 ttctctttgc catggtgatt atatttctaa tttaacgatt ctttgaatgg gtaaaaaaac
     1501 attttgtttc aacaagatgt ggttttctgt aactccccaa attgttgtgt agactacttt
     1561 ttttgcttct tcatcaataa aaaatatttt cactttatgg tgttctgcat ttccaagtgt
     1621 attggcacgc tctaattgag cttttaatgc ttttttctca ctatcactaa gttgtacatc
     1681 ttcttttgga aaatgaaaat ttacaacttc ttctttttca actaacttaa attcagaatc
     1741 cattacacta atattttgag caaaaataaa tgattgataa atacgcaaca aatattttac
     1801 aaagttttaa ataaaaaaag catttttaaa aaaaatcgac gaaatggaag tatttttcta
     1861 caatttacac aaaaaaaaca ccatttaacc taataatcgt ttgataagtc ctaatttttt
     1921 catggaatag ggagaatatt tgatactcaa ctccatccat gttggtttat caataatgct
     1981 tttaaaatgg gaaaaggcat caaaacctgc cttgccatga taggctccca taccactatt
     2041 tccaactccc ccaaaaggta aattgctgtt ggaaatgtgc atcaaagcat cgtttactgc
     2101 tccaccacca aatgaaagtt cgtttaatat tttctctttt tcttgcttat cattcgtaaa
     2161 cacataacaa gaaagtggtt tttccaactg tttgatggtg tgaataactt cgtttaaatg
     2221 ggtgtatgca ataaagggta aaatgggacc aaaaatttct tcttgcataa ctttgtcagt
     2281 aaaatcaaca tcaactaaaa gtgtgggttc aataaaacgt tcttctttat tgtattctcc
     2341 tccaaaataa acagtagatg gcgccattaa ttgtaacaaa cgctcaacat gtttcttgct
     2401 tataatttga acaaaattct tgttttcaat ggaataatgc gccttttgga tttctttttt
     2461 cacagcttct aataactgct tttcaaccgt cttgtgcact accacataat cgggagcaat
     2521 acatgtttga cccgcgttta aaaatttagc ccataccaat cgtttggccg tcatctcaat
     2581 agagcaagat ggagtcacaa tagctggact tttaccgccc aattctaaag ttactggtgt
     2641 gaggtttttt gccgccgctt gataaacaat acgcccaacg tgtgtacttc cagtaaaaaa
     2701 tattttgtca aaacgttctt ctaacaaggc tgttgtaatg cttgcatcac cttcaactac
     2761 ggcaatatag ttttcgtcaa aagcatgagc gattatttga gccaacacac cactggtatt
     2821 gcttgcaact tcacttggct ttaaaaccac ggtgcaacca gccgccattg ctgcaaccat
     2881 aggggctaaa gttaactgta tgggataatt ccacgcacca atgatgagtg aaacacccaa
     2941 cggttcaggt agtatgtaac tcttagctgg aaaattagcc aaattggttc tcaccttttt
     3001 aggtttgctc cattttctta tttttttgat agcttcttta atatcaccat aaaccaaagc
     3061 caactctgtt gtataggttt cgtagtctga ttttttgaaa tctgcaaaaa gcgccttgtt
     3121 tataacttct tcatttgcct gaagtgtttt ttgcaacgtt ttcaattgct ttattctata
     3181 atcaaccgat ttggtagcgt tggaatgaaa aaacaaacgc tggttgctaa tcattgttgc
     3241 gaagctcgac atgctgtaaa atttaataaa ttcaagttta gtctttttat tctaaaaaac
     3301 tacctttgta gttcgaaatt tttagataca caacgatgaa ttatcaattt aaaatagaaa
     3361 aacccaacca acaatacatt caaattacag cagaaacagt tgctttaact gataatcctg
     3421 taatccaatt gccttcatgg cgaccagggc gttatgagtt ggggaatttt gctaaaaatg
     3481 ttaaaggttt taaagttttt aatggtaatg gcaagccagt tgagttttta aaaatttcta
     3541 aagatgcgtg gcaattaaca cttgcaaaag gcgaaacaat aaaaattcaa tatgcctatt
     3601 atgcacattt gctcaacgcg ggttctacgt tttttgatgc cacacaatta tatgttaatc
     3661 ctgtgaactg ttgcgtttat caagtgggcg aagaacaggc tccatgcaca gctgaattgg
     3721 caattccaga acattggcaa attgcaggct ctatgcaaca agaaggaaga aaattgcaag
     3781 cagctaattt tgatgagttg gccgattctc cttttatttg ttctgccaat ctgcaacacg
     3841 atacttatga agtgggtgca acaacttttc atgtttggtt caacggtatt acaaaacctg
     3901 attgggagcg tgttttgcgc gattttaaag cattttctgc caaacaaatt gaaaaattta
     3961 gtgaatttcc agttgatgaa taccactttt taattcacat tctaccaaca aaagcctatc
     4021 acggggtaga acattgcaaa tcaactgtta tttgtttagg accttcctac gatgtttttg
     4081 gaagtttgta taaagaatta ttaggcgtta gctcgcacga gttataccat acttggaacg
     4141 taaaatctat tcgcccgatt gaaatgtttc cctacgattt taccaaagaa aactattcgc
     4201 gtttaggcta cctgtgcgaa ggggttacaa cttatatggg cgatttgttt ttattgaaaa
     4261 gcaaggtttt ttcagtggaa cagtacttgg tggaacttgc cgctcgtttt cgaattcatt
     4321 ttgacaacca cggtagattt aactattcgg ttgccgcgtc ctcctacgac acttggttgg
     4381 atggatacga gccgggagca cccaatcgca aggtttcaat ttataccgaa ggaagtttat
     4441 tggcgtttgt tgccgatatc acgttattaa aaaacaccaa caataaatac ggtttagacg
     4501 aggttatgcg tcgtttgttt ttcaattatg cattacaagg caaaggtgtt tctgagcaag
     4561 attataaaaa tgaattggag gcagtaggca attgctcgtt tgatgactat tttcaaaaat
     4621 ttgtgtgggg caatgaacct tatgaagcaa ttttaaccga tgcgttggaa tacattggtt
     4681 tggagttggt tcaaaaacca gctgctgcat acagtcaagc acgattaggc gttaaatgca
     4741 ttgtggatgg cgacaaaaca gttgctaaga atttttatcc tggcgctcct gcggacatgg
     4801 gcggaatgat ggttgacgat caaattttgg cggttaacgg agtggtgatt aacggaaatt
     4861 ttgaccaatg gttgaactat tttgacgaag atgaaaaacg catcaccgtt atcagagcgg
     4921 ggaaattggt ggaattggta ttgcccgaag taagtcgcaa tttttatttg gaacaccaac
     4981 taaaatgtgt tgaaaatcct aataattttc agaaaaaagg ctttgctgtt tggagcgagt
     5041 aatggtagtg aacacaaata aattcagcac ccatgaacga taattgttca aaattcattg
     5101 cctttcaaaa ggatgtttcg gcaatagcct tgccccaaaa gtttactttt ccgttttatt
     5161 acgaacctca tccgttggct gttattgcca gcgaagaatt gcaagcctac atcaacaaac
     5221 aagaagggtg gcaacacaat tttggattga atcctgccat gacaggaatg gttattggca
     5281 aaatgtttgg cgttttagtt gtgcaaaacc aacaaaatga attggggtat ttggcagcat
     5341 tctctggaaa attagccgat aaaaacgatt atgaacactt tgttccacca gtttttgaca
     5401 tgctgaccga aggaagtttt tatagaaaag gtgaacgtga attgttggaa caatcagcgg
     5461 ttatagagca attattgcaa tcagatgaat taaaattgtt gaaaaacgaa gtagttttgg
     5521 tggaacaaac ctgtcagcaa gaaatcattg attttaaaga aaaagccaaa atatctaagg
     5581 aaaatcgcaa aattcaacga gaaaatgctg aattattgag cgaggaagaa aaaatccgtt
     5641 tgttcaaaga gttggaccga caaagcattc atgaaaattt tgcccttaaa gatttaaaga
     5701 aaatccacct tgaaaaaaaa caagctgcac aagcaaattt ggatgatttt ttgaatcgta
     5761 taaaaacatt gaaagaaaaa cgcaagcaac aatcgcaagc tttgcaaaac caattgtttg
     5821 atcaatacca ttttttaaat gcacgcaatg aaaccagaag tgtgttaagt atttttgaac
     5881 acattgaaaa cggtaaacca ccatcggggg ctggagaatg ttgcgcaccc aagttgttgc
     5941 attatgctta ccaacatcaa ttaaaaccca ttgcattggc tgagttttgg tggggagcat
     6001 cgcccgcatc tgaaattcgc aagcaccaac aattttatcc ttcatgcaga ggaaaatgcg
     6061 aaccaatttt gggacacatg ctgcaaggtt tagatgttga ccctaaccca atgaaaatca
     6121 accctgctga gggaagagaa ttagaaattg cctacgaaga cgaagatatt gtggtagtta
     6181 acaaaccaac cgagttttta tccgttcctg gaaaaaccat tcaagattcg gtgctcaaac
     6241 gcatggaaac ttatttggtg ggcgcatctg gacccttgtt gattcatcgt ctagacatgt
     6301 caacctctgg tattttgttg gtagcaaaaa ataaatttgc ccacgatttc ttgcaacgcc
     6361 aattcatcaa acgttcggta aaaaaacgtt atgtggcttt gcttgaaggt gaattgaaag
     6421 aacatgaagg ttttattgat ttgcctttgc gtgttgattt ggacgacaga cctcgtcagt
     6481 tggtgtgtta cgaatacggc aaacaggcac aaaccaaatg ggaattgatt gaagtgaaaa
     6541 acggaaaatc gaaagtatat ttttacccat tgacgggtcg cacccaccaa ttgcgcgtgc
     6601 atgcagccca tccgcttggt ttaaacatgc ctatcgtggg cgatgatttg tatggaacaa
     6661 aagcaaaccg tttgcacttg cacgccgagc agttaacttt tcgtcatccg cgcaccaaag
     6721 agttgatgac cgttcatgta ccagcagaat tttagaatgt ttaggctgtt ttcaagtttt
     6781 cttatctttg cgctatgaga attgatattt taaccatttt acccgaattg ttagcaggac
     6841 cattttcggc ttctatcatg aagcgcgcac aagaaaaagg ccatgctgag attcacttgc
     6901 acaacatccg cgattattct accaacaaac acaaaaacgt ggatgattat caatatggtg
     6961 gcggtgcagg aatggttatg caaattgaac ctattgcagc tgttattgaa aaactaaaaa
     7021 gtgagcgcca ctacgatgaa attatttaca tgacacccga tggcgaagtg ttggatcaag
     7081 cgatttgcaa tcaattctcg gcacatgaaa atttgatgat tttgtgtggt cactacaaag
     7141 gtgtggatga acgtgtgcgc gaacattaca tcaccaaaga aatttccatt ggttcgtatg
     7201 tgttgtcggg cggtgaattg gctgctgcgg tattggtgga tagcgttgtt cgactaattc
     7261 caggggtact caacgatgaa acatccgctt taaccgatag ttttcaagac aatttgcttt
     7321 cgcctccagt ttatacacgt ccaccagaat ttaatggttg gaaagttcca gatgtcttat
     7381 tatcgggtga ttttaagaaa attgataaat ggcgcgaaga acaagcgttg gaacgcacca
     7441 aacaacgtcg tccagattta ttgaaataat tttattttga aattgcaata cgcactttct
     7501 gtcctttaat cttatgtttt cttaactcag gaagcaattt tccacaactt tttctgtcaa
     7561 ttgccacata ggattgaaaa tccataacag aaatcattcc tactttatct ttgggtaaat
     7621 ttcctttttg gcaaataaac ccgactaagt caattttatt gattttatct tttttacctc
     7681 cactaaaata aatggtcagc caattaggcg cttgatagct ttttttattt gtcagcgcat
     7741 attttttagc ttcaggaagc gaataattaa ctgttggtgc atcgttttca aaaaagtaaa
     7801 tagcgccagt ttcgtgcatg cgtgctgtac gcccattacg atgtgtaaac gaagcttcat
     7861 cttgcggcag ttgatagtga acaatgtgtt gaacacctgc aatgtcaatt cctcttgctc
     7921 caaggtcagt gcaaattaaa acaggcgcac ttccattttt aaatttaatc aacgagcgtt
     7981 cacgttcatc ttgctccatt ccaccatgaa aggcagttgt ggcaatttcc aagtcgtcaa
     8041 aataattttt tagagcatcc acatcttctc taaaattgca aaacaaaatg gtaggttgca
     8101 attgaaagtc aataatcaac tgaaccaatg tttccaattt tcctttggaa gcctccactt
     8161 gataaaaagt ttgctgttta ttttgagtag ttgttacatc aaacgtgaga ggttgtgtaa
     8221 aacgaatgat gggtggaaat tgctctaatt tggtagccga acaataaata ctttttttca
     8281 gctgtcgcaa ttgcttataa atttgttcaa tatcggataa aaagcccaat tctaagcatt
     8341 tatcaaattc atcaataatt aaattaggag taaatgaaaa atcgatgagt tttctgtgca
     8401 ttaaatcagt aattctacct ggcgtaccaa taattacctg cggacgagct gccaaattgt
     8461 tcttttctgt tttaatggca tgtccgccat aacaggtggt taccgaaaaa tttgttttca
     8521 acgatttaaa caccgtttct atttgcaatg ctaattccct ggtaggcgaa agcaccaaca
     8581 attgaggcga attggaagct tcatttttca agtgctcaag gatggcaatt agaaaggtca
     8641 acgtttttcc agaacctgta ggtgcatata aaacaagctc attataaggc tcattggatt
     8701 gaaatacaag ttgttgtatc ttagtaagct ccttatgccc taattttctg agttgttcta
     8761 ccttttcatt catcgtacaa aattggtcat ttatcttaga attcatataa aaatctacaa
     8821 aaaatataca atcgtctaat ttttaatgca attggtcgaa aataatttta aggtgtcgaa
     8881 aatcgtctta catttgtatc ataaaattga tagttaatca aacatgatat aacaatcaaa
     8941 ctagaagtct ttttcagtga ataatagtgg ttaggttagg ttagaaaaaa cggttcagtc
     9001 tttggaccgt ttttttttga tgttaacttg aaaaaatagt tacctttata ttttaataat
     9061 tatctatttt caaaaatggt cgtattattt ttacttttct tttctttggt agcctattca
     9121 caagcacctg caattgatta caacaataaa ttattgtggg aaatttcatc caaaggaagc
     9181 aaacaaaaaa gctatttatt tggcagctat cacaccaacg ataaacgtgt ttttgatttg
     9241 agtgattctg tatattttgc cttaaatcaa gctaaagcga ttgttttgga aacagatgtg
     9301 tatgcaatgt ttaaagattg ggatactcgc cgcgatgatt ttcgtttgaa ttatgacaat
     9361 caaggaaatc cttataccaa ttccaataac tcgtcacgca ctgcttatgg tgatgaagac
     9421 ggaatgcctc aatttttaga tttgtatttt ttgcaatacg gtttcaatgc tcaaaaagaa
     9481 ttttatgtct tggaacccat tgaagatcaa atgaatttaa ccgaaaacac ctccaattat
     9541 ctttatccat acattaatga ttacaccagt tttggtgaaa aatccattaa attatacacc
     9601 gaaggaaata ttgatgctat tgaaaaatta atgcaaacgg gaatgcgttg gaatccagag
     9661 ttttatgaca agcttattct aaaaagaaac gtcgtaatgg ccaacggaat tgatagttta
     9721 ataaaaaatg gaccgttgtt tatctcaatt ggcgcaggac atttgtcagg taataaaggg
     9781 gttatcaatt tattacgtag caagggttac caaatgcgtc cagtactttg gtcaaaaagt
     9841 gcaaccgcaa ctcaagcaaa agtgcaagta caatccttta aaagttatca atatatcgac
     9901 accatcacag ggttaaatgt tacattgcct ggaaaaccat ttgaaattga aaaagaaggc
     9961 gttgtgttag atttgattta tcgtgaaatg ggacaaggaa acacttattc agttgaggtg
    10021 attaaacgcc aagagggaca aactctagaa accgtagcac acgactacat tctaagccct
    10081 ggcgatgcta aaatgaagca agtaattgtt gacaatgagt ttagctacta cgaaggcatt
    10141 tccgattcgt atcccgaagg attagcatgg acgcgcgtta ttgaaaccta tgattatttt
    10201 gcattcatta aagcatacgg gggcaataaa ttcatgaatt ctaaacgacc acaaaagttt
    10261 tttgacaacg tttggtttga gtagggttac tgcaacaatt ccacgtattt tttcaaatta
    10321 gccgatgtag aatagcgatt gataactgtt tgttgtcctt ttaatccaat ttcttttcgc
    10381 aaagtagcat tttccaataa ttgctttaaa tagctataaa attcatcgtt ggatgtagct
    10441 aaaaatccat tcgtttgatg gtcaacaatt gcagtattca caccaacagg cgaaataacg
    10501 caaggaattc cgagtgacat gtattgcaag gctttgaatc cacattttcc tttcgaccat
    10561 ttatcttctt ttagcggcat tacaccaatt gaaaatttca ttaaatcggc tatctctgtg
    10621 tctttattcc attgaatgta acgcaatgat tgcaactcca ttttaggttt ttgattagaa
    10681 atgacaataa aatcgaatga aaattctttt tccaactgag caattaccgg ttccaactca
    10741 tacaagtaat tcatggtagt taatgtgccc gtccacccaa ttgatagccg ttgatgggca
    10801 tagtctatcg tttggttgtg gtaattttcc gtgtccaacg ttgtgggaat aataacaaca
    10861 ttagagttat aaggttgtgc ataatccgcc aaaaattgat tgcccacaga taccttgtgc
    10921 gcccatttga tgcaataagg aactttccaa taacatttta tccattgaac ttttgcgttg
    10981 gatttagaat aattgggcaa ccaaatagca tcgtcaaaat cataaatata gttgactttt
    11041 aacactttag aaataaacca ttcaaaaatt ggtggaccaa aatgtgccat ctcacgatga
    11101 ataaaaacca catccgcttc tttgatttta aacaacacca cccatcgttt aaaaaaactc
    11161 atgagcatta gataagtttt caaaaaataa cgtccttttt gataaagtac attccatgtt
    11221 ttggtgtcaa taaatggaaa gaagtgaaat tcaaaatttt gttgttccaa taaattgatg
    11281 tattgctcgt aacgaaaacg ttgggatgga gcggtatcaa ctggataggg acaaaaaata
    11341 aaaactttct tcttttgcat ggtgttgcaa aaataaactc aaaatttatt taaaggctat
    11401 tttcattaaa ataatgtaaa caatgatgtt acgaccactc gtaattttta aagaaaaaaa
    11461 acaaataccc taaaaaaatg ttaaaaattc ttaaaaattt gagaccgcag acttgaaaaa
    11521 ctaccttcgc agtcaaattt aatatttatg aaacatttta ttaatattaa agtagtcgtc
    11581 ataagtt
//
LOCUS       DAOQVQ010000023        11478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_6344_length_11478_cov_3.521492, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000023 DAOQVQ010000000
VERSION     DAOQVQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6344_length_11478_cov_3.521492"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKN22_01425"
     CDS             <1..789
                     /locus_tag="PKN22_01425"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41393.1"
                     /translation="MLSKPAVQPQIKVNPHFNPFDTPTRGGSTASKNVALNKFGYGEN
                     TTTQQDWENFYKIDELEEQEEEVVQQSFELEETSTKFEQFIVRSPYLIFPSKNGLMVV
                     HGKRAVERITYDDMMREFIVRPIASQTLLFPYEKEISKGEESIWNSQMGLLQRLGFES
                     TIADSMLLLTAVPAVLEEEAINECIDAILDNLAHSSIDKGDIAHILVANISKSIGKSK
                     HLSDNKESVTNLVETLFQCADHSYTPSGKQIIKTILLTDIHQLF"
     gene            792..1550
                     /locus_tag="PKN22_01430"
     CDS             792..1550
                     /locus_tag="PKN22_01430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS41394.1"
                     /translation="MFQNIPVVTKNILFINILVYVAMMVGINTGFENLPFLLSAHYFN
                     APTFEPYQIVTHMFTHDTSNILHILFNMLLLVSFGSHLERIWGPKRYFIFYIASGIGA
                     LVLYNSLGMYELHQIKQALIADGYNIDVVNEFVKGTGTVTNLPVHSDMSQVLFNEYSG
                     MSLSSMAGASGAIFGLLAAFAMLFPNTELMLLFPPIPIKAKYLIGGYLAYEIYMSFFS
                     TTIDNIAHLAHVGGAVVGIIFVIYWRKKDRNHFY"
     gene            1562..2470
                     /locus_tag="PKN22_01435"
     CDS             1562..2470
                     /locus_tag="PKN22_01435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS41395.1"
                     /translation="MRTSTNFVSDLKSTFYSGGMTVKLIFINVLVFIVIGVLEVLARI
                     IGGNVGNGITELTISIFTLSASFKAFIFKPWGLFTSIFTHFGLMHLLFNMLFLYSVGR
                     MFEQFFSAKRLLYTYILGGLAGGIFELVSHLILPGVEGGLVVGASGAVMAILVATAFY
                     QPQLTVSIWGLFNMRLIYLALIFILINLYSAGMGIEDGTAYFAHLGGALLGYFSVKNP
                     FSSNNIINRGIRMGDQLMQIFKKKPKSSFNKSTSNARFKTDEDYNFEAKQRQEKIDKI
                     LDKISKSGYESLTKEEKDFLFRQSNK"
     gene            2470..3588
                     /locus_tag="PKN22_01440"
     CDS             2470..3588
                     /locus_tag="PKN22_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNS41396.1"
                     /translation="MAGKFHWLKHIIRFSNYVRIGSIVLIISLLIAYLTPLIHPENLW
                     LLPFFGLAYPILVAILFITIIYWLLMRKWKWVLALLVLFLIGISYFLRLFAFGEVIET
                     PINAKTSLKILSNNVQIFDLYDENRQQKYQTRDSIFNYAIAQEPDIVCFQEFYNKDNP
                     TDFQTIQLFKKEFKAVDYHQRFIYKPVGRQNFGVIMFSRLPVIAKGDVNFEYQDETNY
                     NFCIYMDVVKNTDTFRIYNAHLQSFRISKIEDDTLKTEQIKGIAEKLRTAYPKRADQA
                     MSIHEHIQNSPYPVIICGDFNDTPVSFVYNQFSSLLTDAFLNCGSGIGATYVGKLPAG
                     RIDYIFHSPALVSTGFNIQKSYFSDHRAVSCTISKINP"
     gene            3588..4319
                     /gene="rluF"
                     /locus_tag="PKN22_01445"
     CDS             3588..4319
                     /gene="rluF"
                     /locus_tag="PKN22_01445"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HNS41397.1"
                     /translation="MSSEELVRLNKYLSELGYCSRRHADKLIDEKRILVNGNLPEMGT
                     KVNRNDSIQVDGEYIQGSATKKVYLALNKPRGIVCTTDTRREKNNIVDFMNYPTRIFP
                     IGRLDKDSEGLIFLTNDGDIVNKILRARYNHEKEYIVRVNHPITRSFIEGMSNGVPIL
                     GTVTQPCVVTQIDRFHFRIILTQGLNRQIRRMCEYFDYRVRELKRIRIMNIHLDTPIG
                     EYREFTEDELTELLRLCEEKENEVD"
     gene            4388..5602
                     /locus_tag="PKN22_01450"
     CDS             4388..5602
                     /locus_tag="PKN22_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNS41398.1"
                     /translation="MQKKSVNRTFLILLLGILAAIGPFTIDMYLPGFQQIATAFQVEE
                     QQVAFTLTSYFFGIAVGQLIYGPIVDKYGRKKPLLVGLSIYIIASLGCAIAFNLESLI
                     AMRLLQALGACVGMVASNAIISDVYEVEKRAKAFSLIMLVMGVAPLIAPSLGSIFIIN
                     YGWATIFYFLALFSFLVFMLIYFFLPETSEHMHSHKLRGKKIALGYYFILRNKVFLNY
                     TLASSLSMSILFGYISSASFIFLTYFGLSKETFSLVFAINASGLISGSYVNGLLTSRF
                     HFIKIAKVAVIVLLAVSGCVLFIVSTTPTLPYQWVVASLFSILFLLGFINPNTTAASF
                     APFSGNAGAASALGGAIRMGVGAITASIVGVLQSDSLVTVFVIILALAVAVVVLTFKA
                     PSIESKLKDTHN"
     gene            5830..7278
                     /locus_tag="PKN22_01455"
     CDS             5830..7278
                     /locus_tag="PKN22_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41399.1"
                     /translation="MFPSLSAYLKFIITNASYFTPKNANNFVELNTKTASILIAYHRN
                     YEAYKIMDRVDSYIVENNPTNKNYLAEFYVLKSNFYIKFNQYSEAKEAAFASLKFKPS
                     VSKYTIDAYNTIGTVYRLDKIYDSSLHFFNKALQLAQTTNNEAWVGILKGNIGYLNYL
                     RGNYKAAKLLIEEDLTTSLRHNQNESAVYAVTFLLKIAGIEKNQQKIETYTAIIDSLQ
                     KLIKTPTVLYEIYKTKAELYTSQGKYKDAVTSFQLKDAYYDSIKVQTSNAKLQSLSFE
                     NRLHREKNANKLLSEQNKRKTQLTITLLVAFILFIIAATSIIILLYKRKQQQRELLEF
                     KISDIQSELDKKKEDLQNVLSSIIQKNEQIKKLKASTKEFEKEEAKEELLVKDQIQSL
                     TLLTESDLNQFNTLFDTAYPNFQNKLINKFPAITNAEMRLAMLLKLNIDKESIATILG
                     ISPESVRKTNLRLRTKLKLKTTNELRKFLNKY"
     gene            7444..10668
                     /locus_tag="PKN22_01460"
     CDS             7444..10668
                     /locus_tag="PKN22_01460"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNS41400.1"
                     /translation="MKLNHFLVLVIIVCSTIIYSWGQVTVTIPSSNYDNGANSDQPYP
                     FGTTNEYVYSVSLYTAAEIGYSGNITKLYWPNRNGYESQNQTIYLKTTSMTSLNSALT
                     FNDYINSATIVNSGTIGSSTASNSLSFTTTTSFAYNGTDNLLVIVKSKRSSTIRNPQV
                     QYTNSTNRHIEWNSSSDPANTTGTLTSKRPNIKMEITSQVQMVCADENCYCSPTYTNT
                     SDHHHSISMTGGSVNLNILNSSHTTQTNAFGNFYGSSDYLEAAAGATITVSNVFHGTN
                     VDYGIWVDWNKNGVFDSSEKVFSQNISGANPTFTGAFTIPAGQANGNYRMRLRGLGGS
                     GTLSPCGNLSWGEARDYKLVVYTPPCTGTPTGGTLTLSATYGAPSSSISGVVSGSTSG
                     VSGLAYQWQYSDDNGSTWIDIAGQTSETLSTTAKNSLGSRQYRRKITCTNSASVAYSS
                     VATYYTTNWPTFTAWFKAEENVTLSSGVSSWGNTSGSVGVYNVTQATGANQPAIIGGA
                     TDYKEFNYHSRIQFDGTNDYLGATAPATDLMSVNGTMFSIFDKASKDGTGFSYAGSAY
                     YQLKPNWRYQTGSTSTGTTSDWTAITIQDIPDPAARLITGIGNNNTQVTRINGEVVPN
                     THSASTTYAPSVGSSNLRFGANGGGGEHVNNSLAEILLMNTTPSAVQIDLVETYLSLK
                     YGLTRGNNIGTAATFNYVATDGTVIFDKTTNAGFTNDIAGIGRDDVSTLNQKQSISVN
                     KNEPATVAIYSGGSIPANNIANGATFDSDKSFFIWGNDGAVPYTDYNNPVCFTNLPAS
                     LGSDPARILRVWKAQSTNFNKTVAVGFETSIILNYTPVSNLRLLVGNSPTDWTAATVY
                     SGATLNGSRVEFLGVTIGNGQYFTLATVNYGATPLPITLTDFSATCSGNSTDLAWTTA
                     SEQNSDYFTVEYSRDGQQWNTISTIEAAGNTANQNNYTLTHNNTIGEGYYRLSLTNFD
                     GKQSILKVISNKCSTASNQLIAYPNPTSGAFTVNITATTNLEATKVIVQDITGRVLIE
                     KTVNLSVGSNQLYFDATTCSNGTYIIAVKNTGEEKFNPIKLVVH"
     gene            complement(10849..>11478)
                     /locus_tag="PKN22_01465"
     CDS             complement(10849..>11478)
                     /locus_tag="PKN22_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNS41401.1"
                     /translation="KKFVMKRYFRILPIFLGLSMLTACGGNTDKSTEEAPKVSYSYDG
                     ASSTMEWTAFKYERKAPVKGTFTTINVQSISKAEDPKTLVESLEFSIPVSSVSTQDVS
                     RDGKIVEFFFKTMATDELKGHIVKLTDDGVAELEVTMNGITDIVTGKYTLEGAHFAFN
                     ATMDIAKWSAQKAIDVLNENCKANHTENGVTKVWTEVDLSFTTHLAESK"
BASE COUNT         3719 a         2047 c         2044 g         3668 t
ORIGIN      
        1 atgttgtcaa aaccagcagt tcaaccacaa ataaaggtca acccacattt taaccctttc
       61 gatacaccaa caagaggcgg ctcaactgct tctaaaaatg tggctctcaa caaatttggt
      121 tatggcgaaa acacaacaac acagcaagat tgggagaatt tttataaaat agacgagcta
      181 gaagaacaag aagaagaagt tgtgcaacaa agctttgaat tggaagaaac ttctacaaaa
      241 tttgaacaat tcattgtgcg ctcgccctat ttaatttttc catccaaaaa cggcttgatg
      301 gtggttcacg gtaaacgtgc cgtggaacgt atcacctatg acgatatgat gcgcgaattt
      361 attgtccgtc cgattgcttc gcaaaccttg ttatttccct acgaaaaaga aatttctaag
      421 ggagaggaaa gcatttggaa cagtcaaatg ggcttgttgc aacgcctagg atttgaaagc
      481 acaattgctg atagcatgtt gctattaaca gctgtacccg ccgttttgga agaagaagcc
      541 atcaacgaat gcatcgatgc tattctagat aatttagccc attcttcgat tgacaaaggc
      601 gatattgcac acattttagt ggccaacatt tcaaaaagca taggaaaaag caaacacctt
      661 tcagacaaca aggaaagtgt taccaacttg gtggaaacgt tgtttcaatg cgccgaccat
      721 agttatacgc ctagcggtaa acaaattatt aaaacaattt tattaaccga tattcatcaa
      781 cttttttaaa tatgtttcaa aatattccag ttgttaccaa aaacatttta ttcatcaaca
      841 ttttggtgta tgttgcaatg atggttggca tcaataccgg ctttgaaaat ttgccttttt
      901 tattgagtgc ccattatttt aacgccccta cgtttgagcc ctatcaaatt gtaacccaca
      961 tgtttacaca tgatactagc aatattttgc atattttatt caacatgttg cttttggttt
     1021 catttggttc gcaccttgaa cgtatttggg gtccaaaacg ctatttcatt ttttacattg
     1081 caagtggaat tggcgcattg gtactttaca actcattggg aatgtatgaa ttacaccaaa
     1141 tcaaacaagc gttaattgca gatggctaca atattgatgt agtcaacgaa tttgtgaaag
     1201 gtacaggaac tgtaacaaac ttgcccgttc attccgacat gagtcaggtg ttgtttaacg
     1261 aatacagcgg catgagttta agttcaatgg caggtgcttc aggtgctatt tttgggttgt
     1321 tagcagcttt tgctatgtta tttccaaata ccgaattgat gttattgttt ccacccattc
     1381 caattaaagc aaagtatttg attggcggtt atttagcata tgaaatttac atgagtttct
     1441 tctctacaac tattgacaat attgcccatt tggcgcacgt tggtggtgct gttgtaggta
     1501 ttatttttgt aatttactgg cgcaaaaaag atagaaacca cttttattaa aatatttgga
     1561 aatgcgaacg tcaactaatt ttgttagcga tttaaaaagc acgttttatt cgggtggaat
     1621 gaccgtaaaa ttgatattca tcaacgtgtt ggtgtttatt gtaattggtg ttttagaagt
     1681 tcttgctcgc attattggtg gaaacgttgg caatggcata acagaattaa ccatttctat
     1741 ttttacacta tcggcttcat tcaaagcgtt tatttttaaa ccttggggac tttttaccag
     1801 cattttcacg cattttggat tgatgcacct tttgtttaac atgctatttt tgtatagtgt
     1861 tggtaggatg tttgaacaat tttttagcgc caaacgtttg ttgtacacct atattttggg
     1921 gggcttagct ggcggtattt ttgaattggt ttcgcattta attctaccag gagttgaagg
     1981 tggattggtt gttggcgcat cgggggcggt aatggctatt ttggtggcaa ctgcttttta
     2041 tcaaccacag ttaacggttt ctatttgggg attattcaac atgcgcttga tttatctggc
     2101 cttgattttc attcttatca atttgtatag tgctggaatg ggcattgaag atggaaccgc
     2161 ttattttgca catttgggtg gtgctttgtt gggttatttc tcggttaaaa acccatttag
     2221 ctccaacaac atcatcaacc gtggaataag aatgggcgat caattgatgc aaatttttaa
     2281 aaagaaaccg aaatcttcgt tcaataaatc aacaagcaat gctcgtttta aaaccgacga
     2341 agattacaac tttgaagcaa aacaacgcca agaaaaaatt gataagattt tagataaaat
     2401 ttccaaatcg ggatatgaat cactgacaaa agaagaaaaa gactttttgt ttagacaaag
     2461 taataagtaa tggctggcaa atttcattgg ttgaagcata tcattcgttt ttctaactac
     2521 gttaggatag gctccatcgt gttgataatt tccctactta ttgcttacct tactccgtta
     2581 attcacccag aaaacttgtg gcttttacca ttttttggac tagcctaccc tattttagtt
     2641 gcaattttat tcatcaccat catttattgg ctgttgatgc gcaaatggaa gtgggtgctt
     2701 gcgctgctgg tgcttttttt gattggcatt agctatttcc ttcgtttgtt tgcatttgga
     2761 gaagttattg aaacgccaat aaatgccaaa acaagtctaa aaatcttgtc aaacaacgtg
     2821 caaatatttg atttatacga cgaaaatagg caacagaaat accaaacacg cgatagtatt
     2881 ttcaattatg ccattgcaca agaacccgat attgtttgtt ttcaagagtt ttataacaaa
     2941 gacaatccaa ccgattttca aaccattcaa cttttcaaaa aagaatttaa agcagttgat
     3001 tatcaccagc gttttatata caaacctgtt ggtcgacaaa attttggggt catcatgttt
     3061 tcacgtttgc ccgttattgc caaaggagat gtgaattttg aataccaaga cgaaaccaat
     3121 tacaattttt gcatctatat ggatgtcgtt aaaaacaccg atacatttcg catttacaac
     3181 gcccatttgc aatcatttag aattagtaaa attgaagacg acactctaaa aacagagcaa
     3241 attaaaggaa ttgcagaaaa attaagaact gcctatccaa aaagagccga ccaagcaatg
     3301 agcatacacg aacacattca aaattctccc tatccggtta ttatttgtgg cgatttcaac
     3361 gacacacctg tttcatttgt ttacaaccaa tttagcagtc ttttaaccga tgcatttttg
     3421 aattgtggaa gcggaattgg cgccacttat gttggtaaat tgcctgccgg aagaattgac
     3481 tacatttttc attcaccagc attggtttca accggtttta atattcaaaa aagttatttc
     3541 agtgatcacc gtgcagtttc ttgtaccatt agtaaaatca acccataatg agcagcgaag
     3601 aattagttag attgaacaaa tatttgagtg aactaggtta ttgttcgcgc cgtcacgccg
     3661 acaaactcat tgatgaaaaa cgtattttgg taaacggtaa tttacccgaa atgggaacaa
     3721 aagttaaccg caacgacagc attcaggtgg atggtgaata cattcagggt agcgccacca
     3781 aaaaagttta tttggcactc aacaaaccac gtggaattgt gtgcacaacc gacacccgaa
     3841 gagagaaaaa caacattgtg gattttatga attatccaac acgtattttt cctattggtc
     3901 gactggataa agacagtgaa gggttgattt ttcttaccaa cgatggcgat attgtcaaca
     3961 aaatattgcg tgcacgctat aaccacgaaa aagaatacat tgttcgcgta aaccacccga
     4021 ttacacgttc atttattgaa ggaatgagca atggagtgcc aattttagga acagtaaccc
     4081 aaccgtgtgt ggtgacacag atcgatcgtt ttcatttccg cattatttta acgcaaggac
     4141 ttaatcgtca aattcgtcgc atgtgtgaat attttgatta tcgtgttcga gagttaaaac
     4201 gtattcgcat catgaatata catttggaca ctccaattgg cgaataccgt gaatttacgg
     4261 aagatgaatt gacagagttg ttgcgccttt gcgaagaaaa agaaaacgaa gtcgattaaa
     4321 tttgtacata tcgacatatc gtaatattgc gatatgataa aattagccgt atttttgcac
     4381 cctcgttatg caaaaaaaat cagttaatcg aacatttctt attcttcttt taggaatcct
     4441 tgcagccatt ggtccgttta ccattgacat gtatttaccc ggatttcaac aaattgcaac
     4501 agcatttcag gtagaagaac aacaagtagc ctttacgcta acatcctatt tttttggaat
     4561 tgctgtgggg caattaattt atggtccgat agtggacaaa tacggtagaa aaaaaccatt
     4621 gcttgttggg ttatccatct atatcattgc ttctttaggt tgtgcaattg ctttcaattt
     4681 agaatcactc attgccatgc ggttgttaca agctttaggt gcatgtgtgg gaatggtggc
     4741 ttccaatgct attattagcg atgtttacga agtagaaaaa cgggctaaag cattttcatt
     4801 aatcatgttg gtgatgggag ttgccccgtt aattgcaccc agtttaggaa gtatttttat
     4861 aatcaactat ggttgggcga ccatttttta ctttcttgcc ttgttttcat ttttggtttt
     4921 catgttgatt tattttttct tgcccgaaac aagtgaacac atgcacagcc ataaattacg
     4981 tggaaagaaa atagcattgg ggtattattt cattttgcgc aacaaagtgt ttttaaatta
     5041 caccttggca agtagtcttt ccatgtcgat tttgtttgga tacatttcat ctgcatcgtt
     5101 tatatttttg acctattttg gattgagtaa agaaaccttt tcacttgtat ttgccatcaa
     5161 tgcttcgggg ttaattagtg gaagttatgt gaatgggtta ctcacctccc gctttcattt
     5221 tataaaaatt gccaaagtgg cagttattgt tttactagct gttagcggtt gtgtgttgtt
     5281 cattgtttct actacgccaa ccctgcccta tcaatgggtg gtagcaagtt tgttttccat
     5341 tttattttta cttggcttta tcaatcccaa taccacagcg gcatcatttg ctccattttc
     5401 tggaaatgca ggagcggctt cagctttagg aggtgcaatt agaatgggag ttggagcaat
     5461 aaccgcttcc atcgttggag tattacaaag tgattcactt gtaactgttt ttgtaattat
     5521 acttgcgctt gctgttgcag ttgttgtgct tacgtttaaa gcaccatcaa ttgaatccaa
     5581 actgaaagac actcacaatt agtacgtttc ataagaaaat taaatgttct atatttgctg
     5641 aaaaacaaat tatctattat tttgaattgc aaactggtta tacaattact aatactcatt
     5701 ccattatttt tttggaacag gaattattgt aatgcacaaa acgattcatc actaccacat
     5761 aaagccccac taatcataga agatgcaaag actttttccg attttgtagc ttcagatagc
     5821 aacaaaaata tgtttccatc gctatcggcc tatttaaaat ttataataac caatgcttcc
     5881 tattttactc caaagaatgc caacaatttt gttgaattaa acacaaaaac ggcttcaata
     5941 ttaattgctt accatcgtaa ttacgaggcc tataaaatta tggatcgagt tgattcttat
     6001 atagttgaaa acaatccaac aaacaaaaat tatcttgcag aattttatgt tttaaaaagt
     6061 aatttttata tcaaattcaa ccaatattca gaagcaaaag aagcagcatt tgcatcgcta
     6121 aaattcaaac ctagtgtaag taaatatacc attgatgctt acaacaccat aggaaccgtt
     6181 tatagattgg ataaaatcta tgattcttct ttacactttt tcaacaaagc attgcaattg
     6241 gcacaaacaa ccaacaatga ggcttgggta gggattttaa aaggcaacat tggctatctc
     6301 aattatttaa ggggaaatta caaagctgca aaactattaa ttgaagaaga tttaactaca
     6361 agtttaagac acaatcaaaa tgaaagtgct gtttatgcag taacttttct tttaaaaatt
     6421 gcaggaatag aaaaaaatca gcaaaaaatt gaaacttata ctgctattat tgattctttg
     6481 caaaagttga ttaaaacacc tacggttttg tatgaaatat ataaaacaaa agcagaattg
     6541 tacaccagcc agggtaaata caaagatgcc gtaacatcgt ttcaactaaa agacgcatat
     6601 tacgatagta ttaaagttca aaccagcaat gcaaaattgc aaagtctaag ctttgaaaat
     6661 agattgcatc gtgaaaaaaa tgccaataaa ttgttgagcg agcaaaacaa gcgaaaaacc
     6721 caattaacaa taaccttact tgttgccttt atccttttca ttattgcagc tacttctatt
     6781 attattttat tgtataaacg caagcaacaa caacgcgaat tgttagagtt taaaataagt
     6841 gatattcaaa gtgaattaga caaaaaaaag gaggatttac aaaacgtatt gagtagcatt
     6901 attcaaaaaa atgagcagat taaaaaatta aaagcaagca ccaaagaatt tgaaaaagaa
     6961 gaagctaaag aagaattatt ggttaaagac caaatccaat cgcttacact tttgacagaa
     7021 agtgatttga atcaattcaa cactttgttt gacactgctt atccgaattt tcaaaacaaa
     7081 ttgattaata aatttcctgc tatcaccaat gccgagatgc gtttggctat gttactcaaa
     7141 ttaaacattg ataaagagag tattgctaca attttgggta tttctcccga atctgttcga
     7201 aaaacaaatc taagattgcg cacaaaatta aaattaaaaa caaccaacga attacgcaaa
     7261 ttcctcaata aatattgatg ttttgacgat tttataattg tcccgttttt gtctcttttt
     7321 tgtctttttt ttgtctcata gtatattttg tcacaaaatc gtaacccctt aattttgttt
     7381 cgtaaaaact catttaattg acttcttaaa ttttctcaaa gtttaaggta aaaacgaaat
     7441 actatgaaat taaatcattt tcttgtttta gtaataattg tttgctctac tattatttat
     7501 agttgggggc aagttactgt tactattcct tcaagcaatt atgacaatgg agctaattca
     7561 gaccagcctt atccatttgg tacaacaaac gaatatgtat attctgtatc tttatatact
     7621 gccgctgaga ttggttattc tggtaacatc actaagttat attggccgaa tagaaatggt
     7681 tatgaaagcc aaaatcaaac aatatatctt aaaacaactt caatgactag tttaaatagc
     7741 gcattaactt ttaatgacta tataaatagt gcaactattg ttaattctgg taccattggt
     7801 agttcaacag cttcaaattc tttatcattt acaactacaa ctagttttgc ttacaacggc
     7861 accgataatt tattagtgat agtgaaaagc aaaagaagct caactataag aaaccctcaa
     7921 gttcaatata ccaattcaac caaccgtcat attgaatgga attcatcttc tgacccggct
     7981 aacacaactg gtactttaac aagcaaaaga ccaaatatta aaatggaaat aacaagtcaa
     8041 gtgcaaatgg tttgtgctga tgaaaattgt tattgttcac ctacatacac taatacatca
     8101 gatcatcacc attcaatctc aatgactggt ggtagtgtca acttaaatat tttaaattca
     8161 tcacatacga cacaaaccaa tgcttttggt aatttttacg gttcttccga ttacctagag
     8221 gctgcagctg gtgccacaat tacagtatca aatgtttttc atggtacaaa tgtggattat
     8281 ggtatttggg tagattggaa taaaaatgga gtttttgata gcagtgaaaa agttttttct
     8341 caaaatatat caggcgctaa tcctacattt acaggagcat ttacaattcc tgcaggtcag
     8401 gcaaacggaa attatagaat gcgtttacgt ggattgggtg gttctggaac tctttcacca
     8461 tgcggaaatc ttagttgggg cgaagcaaga gattataaac tagtagttta cactccacct
     8521 tgcacaggca caccaacagg tggtacttta acgctttcgg ctacttatgg tgcaccaagt
     8581 tcttcaattt cgggcgttgt ttcaggtagc acttcgggtg tatctggttt agcataccaa
     8641 tggcaatatt cagatgacaa tggctcaaca tggattgata ttgctggaca gacctctgaa
     8701 actctttcta caactgcaaa aaatagtttg ggaagccgcc aatacagaag aaaaattaca
     8761 tgtaccaatt cggctagtgt agcctactca tctgttgcaa cctactacac aaccaactgg
     8821 ccaactttca cagcttggtt caaggctgaa gaaaatgtta cattaagctc aggtgtaagt
     8881 agctggggaa atacgagtgg atcagtaggg gtttacaatg ttacccaagc aactggagct
     8941 aaccaaccag cgataattgg tggcgcaaca gattacaaag aatttaatta ccattcacgc
     9001 attcaatttg atggtactaa tgattattta ggagctacag cacctgcaac agatttgatg
     9061 agtgtaaatg gcacaatgtt ctctattttt gataaagcat ctaaagatgg aacaggattt
     9121 tcgtatgctg gctctgccta ctatcaattg aagcccaatt ggcgttatca aacaggaagc
     9181 acttctactg gaacaaccag tgattggact gcaataacca ttcaagatat acctgatcct
     9241 gcagcacgtt taattactgg aataggtaat aacaatacgc aagtgactcg aattaatgga
     9301 gaagttgttc caaacacaca ttctgctagc acaacgtatg ctccatcagt tggttcatcc
     9361 aacctccgtt ttggtgctaa tggcggtggt ggtgaacatg ttaacaattc acttgcagaa
     9421 atattattaa tgaacacaac tccttctgct gtgcaaattg atttagttga aacttattta
     9481 tcgttaaaat atggcttaac acgtggcaat aacattggga cagctgcaac attcaattat
     9541 gtggctactg atggaactgt catttttgac aaaacaacaa atgctgggtt tacaaacgat
     9601 attgctggaa ttggtcgcga tgatgtaagc actctcaacc aaaaacaatc catttcggtg
     9661 aataaaaacg aacctgcaac ggtagccatt tattctggag gttctattcc tgccaataac
     9721 atagcaaatg gtgcaacttt tgacagtgat aaatcatttt ttatttgggg aaatgacgga
     9781 gcggtacctt acacggatta caataatcct gtgtgtttta ccaatttgcc agcttcttta
     9841 ggatcggatc cagcacgtat tttacgcgta tggaaagctc agtcaactaa tttcaataaa
     9901 acagttgcgg ttggttttga aacttctatt attttaaatt acactccagt atctaacttg
     9961 cgtttattgg ttggaaatag tccaactgac tggacggctg ccactgttta ttcaggcgca
    10021 acattaaacg gttcaagagt tgaattttta ggtgtgacaa taggaaatgg acagtatttt
    10081 acattggcaa cagttaatta cggagcaact ccacttccaa ttacacttac agattttagt
    10141 gcaacctgta gtggtaattc tacagattta gcgtggacca ctgcatcaga acaaaattca
    10201 gattacttta cagtagaata cagtcgcgat ggacaacaat ggaatactat ttcaacaatt
    10261 gaagcagctg gaaatacggc aaatcaaaat aattatacat taacacacaa caatacaatt
    10321 ggagagggtt attaccgatt gagcttaacc aattttgatg gaaaacaatc cattttaaaa
    10381 gttatttcta acaaatgttc aacggctagc aaccaactaa ttgcctatcc aaacccaaca
    10441 tcgggtgcat ttactgtaaa cattacagca acaacaaatt tagaagcaac aaaagtgata
    10501 gtgcaagata ttactggtcg tgttttgatt gaaaaaacag tgaatttaag cgttggtagc
    10561 aaccaattgt actttgacgc aacaacatgt agcaatggaa catacatcat tgcagtaaaa
    10621 aatacgggtg aagagaaatt taacccaatt aagcttgtcg tacactaatc aagtagagag
    10681 aacgagactc taacacaatg gagtctcgtt ttcaaaacaa cataaataaa cgactttaaa
    10741 catgaaaaaa agtatttaag attatatagt agtagtagta tgtttaggta agaaaaaagg
    10801 atttcaacta agttgaaatc cttttttgtt tgtgtaaatg tctaattctt atttactctc
    10861 tgccaaatgc gttgtaaacg ataaatctac ttctgtccaa acttttgtta caccattttc
    10921 ggtgtggtta gctttgcaat tttcgttcaa cacatcaatt gctttttgag ccgaccattt
    10981 tgcaatgtcc atcgtagcat taaatgcaaa gtgtgctcct tctaaagtat attttccggt
    11041 tacgatatca gtaattccgt tcatggttac ttccaattct gcaacaccat catcggttaa
    11101 tttcacaata tgacctttca actcatctgt tgccattgtt ttaaagaaaa actcaacaat
    11161 ttttccatct ctggaaacgt cttgcgtaga aaccgaacta acaggaattg aaaattctaa
    11221 agactcaacc aaagttttag gatcttctgc ttttgatatc gattgaacat taattgttgt
    11281 aaacgttcct ttaactggtg ctttacgttc gtatttaaaa gcagtccatt ccattgttga
    11341 acttgcaccg tcgtagctat acgaaacttt aggagcttct tctgttgatt tatctgtatt
    11401 accaccacat gcagtcaaca tgcttaatcc caaaaagatg ggtaaaattc taaaatatct
    11461 tttcataaca aacttttt
//
LOCUS       DAOQVQ010000024        11441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_6374_length_11441_cov_3.744072, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000024 DAOQVQ010000000
VERSION     DAOQVQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_11441_cov_3.744072"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PKN22_01470"
     CDS             <1..1295
                     /locus_tag="PKN22_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41402.1"
                     /translation="DERRIYLYEDSTGNGDIYFSDLKLNKFDEIQLLKDKNINTESWE
                     THITVSPDGQLMIFASDRPGGLGGLDLYYCKKTEDGKWTDPVNMGPNINGPKDDDAPF
                     ISVNNKQLYFGTNDARSMGGFDILISEKGEDGTWGVARNIGYPFNTTNDDLFYTTTVD
                     GLTGYITSYRADGKGEKDIYEIKNDFLGVKGHTVLKGEVVTADGSLLPENLVFMAEVA
                     CLDCEDGAMKHVLYTRKRDGRFMTGLQPCKTYTLTYYDATDKKEMGKETFKTECTTEY
                     REVHKVLRIDVEKREVIFPDTIPDVVVKDYTNIELKHYFDYNKNKLTVKKGELKDFVK
                     AIDTQLADGREKVTIVIESSASYVPTKTFDSNEELAKLRAENMKYDLLDYFDKNEATK
                     GKVTIVISKAVVQGPEYVKDRNNTEKYKPYQYVFLKTE"
     gene            1424..2245
                     /locus_tag="PKN22_01475"
     CDS             1424..2245
                     /locus_tag="PKN22_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS41403.1"
                     /translation="MSNNLLKGKRGIIFGALNNQSIAWKVAERAHEQGATFVLTNAPI
                     AMRMGEINELAEKTNSKIIPADATSVEDLEKLIAESMEILGGKLDFILHSIGMSVNVR
                     KGNHYTSENYDFTMKGFDVSALSFHKVLQTAMKLDAMNEWGSILALTYIAAQRIFPDY
                     NDMADNKAYLESIARNFGYHYGQAKKVRVNTISQSPTMTTAGSGVKGFNEFINYSELM
                     SPLGNAKADDCANYCIAMFSDLTRYVTMQNLFHDGGFSSTGVTEAVINRFTDTNL"
     gene            2263..2820
                     /locus_tag="PKN22_01480"
     CDS             2263..2820
                     /locus_tag="PKN22_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNS41404.1"
                     /translation="MIKLTSIISISGKPGLYKVVAQAKNSVIVESLEDNKKIPAYATD
                     RISALEDISIYTYNEEKPLKEIYADIYKKENGGATISHKEDAKKLQAYFLEILPEYDQ
                     ERVYVSDLKKLFQWYNLLHKSGNLVLEEEVSEEATAKKAEAPAEKKEKKAAPAAKKPV
                     AAKATPKPKTAAAKPKTTVQRKSGS"
     gene            2894..3934
                     /locus_tag="PKN22_01485"
     CDS             2894..3934
                     /locus_tag="PKN22_01485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNS41405.1"
                     /translation="MTYIKGFNAIRAFSVLLVVLTHLDLSEWIPNTDFVMIRVWPMMS
                     GEFGVLMFFVLSGFLITKILLHEKDKFGFISLKKFYIRRFLRLFPPLILFFILMGIAY
                     WNGLMQDAVLSVGYGIFYVYNFVPKSLYFPEMGHLWSLGVEEQFYILWPFVLTVFKKM
                     TSIKWIVYSMVVISGFLTFWFANSLLSQNYFTERWFIPASTPILIGCLAAIVHHTQPQ
                     LTLEICSSKWLPVIMALCFAFTLYVPSELVKIGFLFRAIAVALLLLYLVHNQQKKWMQ
                     ILEWKPLVYVGTISYGIYIYQGFFLSTGGGSEIWFQKFPQNISLAVLLAILSYELMEK
                     PILRYKKKFETK"
     gene            4010..4174
                     /locus_tag="PKN22_01490"
     CDS             4010..4174
                     /locus_tag="PKN22_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41406.1"
                     /translation="MKNPLLLAVLPALLIGLLNGYLLEKKGYNRWQYTFYGFLVSYIF
                     LVKSIAYFLE"
     gene            4174..4896
                     /locus_tag="PKN22_01495"
     CDS             4174..4896
                     /locus_tag="PKN22_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNS41407.1"
                     /translation="MTVFNSYIQAFQQLGFYLSNGAIWKFIIPSVVVTVFYGLLFLMF
                     SFILAPLNLLTFLPWVGDFIQNTYDWLWNSSLSVVFYAQSFTVLTLLSPFMSLLSSKC
                     DEMTTGFVDEYSTARLISDLWRAVKLNIRLFILYLITAAVILWLIQPLLGETVAKGLS
                     YLLSSYLFGFSLFDYNFERYQMNISTTFKKGNTNWIVLLIGGTLFQFIYVIPILGTLF
                     APFLVTIITTHVFITTKDSWIR"
     gene            complement(4958..5437)
                     /locus_tag="PKN22_01500"
     CDS             complement(4958..5437)
                     /locus_tag="PKN22_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41408.1"
                     /translation="MKNFKLILSVMFVFFVAQLNTMAQTEKKPTKEETMEWIAGKLKE
                     HLTGGREFVSYNDGLFSYKKVLSSDVCVTTIDLNKVKSMSNIYSKDFFIQGDGMLTVK
                     CDNSVGNGKNAVSISGQNFNKYTAPFYVVDDPLIERLKKAFKTLIEYNSPKDANEAF"
     gene            complement(5515..6522)
                     /locus_tag="PKN22_01505"
     CDS             complement(5515..6522)
                     /locus_tag="PKN22_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HNS41409.1"
                     /translation="MTDRRNFLKLSALASVAMGLKPLQSFATEHPETKPKKKTINKPI
                     VLSTWRFGIPANEAAWEVLKNNGRALDAVEKGVHVPEADPNERSVGYGGRPDRDGRVT
                     LDACIMDEFSNIGAVACLEHIKHPISVARAVMEKTPHVFLVGEGALDFALSQGFKKEN
                     LLVEASEKEWKEWLKNSEYKPVANIENHDTIGMIALDAHGNLSGACTTSGMAFKMHGR
                     VGDSPIIGAGLFVDNEVGAATATGHGEEVIRTVGSHLVVELMRQGLSPQKACEEAVNR
                     IIKLVKLRNKKLEDIQVGFIALNKQGEHGAFCIQNGFQFAKYDSSGNVLIDANYVIKV
                     S"
     gene            complement(6624..7571)
                     /locus_tag="PKN22_01510"
     CDS             complement(6624..7571)
                     /locus_tag="PKN22_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNS41410.1"
                     /translation="MTLWLIFIAFIVVALALDLGVFNRSPHEIKTKEATIWTIVWVTV
                     AVLFSGVIYLAFENKWIDNPTNLTPTNAVVKYITGYLIELSLSVDNIFLIALIFSSFQ
                     IAKKYQHEVLFYGVLGAIVFRALMIIFGVALITKFDWIIYVFGAFLIFTAIKMLISHD
                     KPKNPKDSKLYKWIKKVYPVSRSVYGGKFFVKRGKLKFATPLFVALIIIELTDLFFAI
                     DSIPAILAITADPFIVFSSNILAVMGLRSMYFLIVGMLDKFKYISYSLIVILAFVGVK
                     MLIVHHVDIPDWLSLGVIVLALAGGMLASKWAPKEETHH"
     gene            complement(7584..8225)
                     /locus_tag="PKN22_01515"
     CDS             complement(7584..8225)
                     /locus_tag="PKN22_01515"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNS41411.1"
                     /translation="MDVILSFFENLMNPDWIMHNGGLYLVLAILFIETGVFFGFVLPG
                     DPLLFISGIVLASVSEAHYPFENPLYNLPFWMALFVLSTVLGNFFGYWFGNKFKFLID
                     RKHDTWFLKQKHLQTARDFYDKKGGFTIAVARFLPIIRTFAPIVAGTINMNVQKFAIY
                     NVIGAVLWVGSLTTLGYLLGDIPWVKHNLEFIILGIVLVVTTPVIVKFFFKKQ"
     gene            complement(8274..8522)
                     /locus_tag="PKN22_01520"
     CDS             complement(8274..8522)
                     /locus_tag="PKN22_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNS41412.1"
                     /translation="MKCPNCNVNLVMSERSGIEIDYCPDCRGVWLDRGELDKIIERSN
                     GATSNIPTYQQHDKKQYSKDSDYHHKKKKSIFSELFDF"
     gene            complement(8607..9347)
                     /gene="bla"
                     /locus_tag="PKN22_01525"
     CDS             complement(8607..9347)
                     /gene="bla"
                     /locus_tag="PKN22_01525"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119059468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HNS41413.1"
                     /translation="MKSLVILLFLIIASHLSLARAPKTKVVYQSKSLVIQQLTKHTYV
                     HTSYLQTNDFGNVPCNGLIVTNKHTSVIFDTPADSASSEELIQWITNTLHSQIKAIIP
                     THFHADCLGGLTAFHHHQIPSYASSKTIAFATKNKSNIPQYSFNDSLVFWLGKKEVTV
                     NYFGAGHTLDNVVGYFPSEKVLFGGCLVKELNAGKGNLEDASVADWPTTIQKVKTVFP
                     KVKYVVPGHGKIGTVDLLDYTQQLFQAP"
     gene            complement(9358..10755)
                     /locus_tag="PKN22_01530"
     CDS             complement(9358..10755)
                     /locus_tag="PKN22_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNS41414.1"
                     /translation="MIKIGFTLLISIIVWNVSAQTTTIDHTIQSTVFGGERKISIFLP
                     EAYEKQKTVAPFHVAYLLDGQFEPYFTITKGIIDYYAQSQQGIPLIVVAIHTQNRWDE
                     FVPTCGDLQNDTIGAQRFTRFLAKEVIPTIDSMYSTSDFRLGIGHSLGGTYVLHELFK
                     DQSLFDATIAISPNLLVCDEQIIRQAANYLKNNPSNHKFIYTSIGTEGKVEKMFLPGV
                     QQLDAIFNSLTPKNWYWQSVQLDNSNHMTTFVPSINDAYLKISSKLMLLESDLIKMAE
                     DSTQSFGEQMVQFYTQMTQFSKSNNEFSPKDAISVLYKLAQLPYDETTIQYADFIAKR
                     FKNTSFSSDERMEFDKDIVDVKNRACFNQIIKQATSYKEQGNLKKASNLYEKAFELNV
                     VRGTHNERLAAVSIFAQTGKIEAAFEQLDLLANKFKLGGNDFFISDETLLPLHQDKRW
                     QQYMNQFVENSKLYR"
     gene            complement(10820..>11441)
                     /locus_tag="PKN22_01535"
     CDS             complement(10820..>11441)
                     /locus_tag="PKN22_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNS41415.1"
                     /translation="SNTSFIGFPLIMAYYTEQDLSIAIICDQVLFLLLSTVGIICAIK
                     GNRSETEGIKAKSILKRLVSFPPLIGCISALALSFFVDLTPAEPFFDKLVATVGPLAL
                     FSVGLQLKFKGWRKQINQILTATFYKLILAPLLVFIAALLIGVHGQIARISVFEAAMP
                     TLVTGSLIAEQFHLNEKLINLIIGVSIVVGFFTSWAWSFIMEWVAL"
BASE COUNT         3733 a         1922 c         2098 g         3688 t
ORIGIN      
        1 cagatgaacg aagaatttat ttatacgaag attcaactgg gaatggtgat atttattttt
       61 ctgacttgaa attgaataag tttgatgaaa tacaactatt aaaagataaa aatataaaca
      121 cagaaagttg ggaaactcat atcacagttt caccagacgg tcaattgatg atttttgctt
      181 cagacagacc aggcggttta ggcggattgg atttgtatta ttgtaagaaa actgaggatg
      241 gaaagtggac agaccctgtg aatatgggac ctaatattaa tggaccaaaa gacgatgatg
      301 caccgtttat ctcagtaaat aataagcaat tgtattttgg aacaaacgat gcgcgttcaa
      361 tggggggatt tgatattttg atttctgaaa aaggagaaga tggaacttgg ggagttgcaa
      421 gaaatatagg atatccattt aacactacca acgatgattt gttttacacc acaacagtgg
      481 atggtttaac aggttatatc acttcgtatc gtgcggatgg taaaggtgag aaagatattt
      541 atgaaattaa aaacgatttc ttaggcgtta aaggacacac agttcttaaa ggagaagtgg
      601 taactgcgga tggttcattg ttaccagaaa acttagtgtt tatggctgag gttgcatgtt
      661 tagattgtga agatggcgct atgaaacatg tgttgtacac tcgtaaaaga gacggacgtt
      721 ttatgacagg attgcaacca tgtaaaacat acacattgac ttattatgat gcaacggaca
      781 aaaaagaaat ggggaaagaa acctttaaaa ctgaatgtac aaccgaatac cgtgaagtac
      841 acaaagtttt aagaatcgat gttgaaaaac gtgaggttat cttcccagat actattccag
      901 atgtggttgt aaaagattac accaatatag agttaaaaca ctattttgat tataataaaa
      961 acaaattaac tgttaagaaa ggtgagctaa aagattttgt taaagcaatt gatactcagc
     1021 tagcagatgg tagagaaaaa gttaccattg ttattgagtc atcagcatct tatgtaccta
     1081 ctaaaacatt tgattcaaat gaagagttag caaaattacg tgctgaaaac atgaaatacg
     1141 atttgttgga ttattttgat aaaaacgaag caacaaaagg taaagttacc attgttattt
     1201 caaaagcagt ggttcaagga ccagaatatg taaaagacag aaacaataca gagaaatata
     1261 aaccttatca gtatgtattc ctaaaaacgg aatagtaaaa accaaagagg ctaacgaaaa
     1321 ttagcctctt tttttgttat taaaagtaga atgtaaaaaa ctctttgata ttttctactc
     1381 gtttattcaa tcattcttgt aactttgtca caaagcattt aatatgtcaa ataatttatt
     1441 aaaaggaaaa agaggaatca tctttggagc attgaacaat caatcaattg cttggaaagt
     1501 tgctgaacgt gcacacgaac aaggtgctac attcgtttta acaaacgcac ctattgcgat
     1561 gcgtatgggt gaaatcaatg aattggccga aaaaacaaat agcaaaatta taccagcgga
     1621 tgccacttct gttgaagatt tggaaaaatt aatcgcagag tcaatggaaa ttttaggtgg
     1681 aaaattagat ttcatcttgc attctattgg tatgtcggtt aacgtacgta aagggaatca
     1741 ttatacaagt gaaaattacg actttacaat gaaaggattt gatgtttcgg cattgtcttt
     1801 tcataaagta ttgcaaacag ccatgaagtt ggatgcaatg aacgaatggg gttcaatttt
     1861 agcgttgact tacattgcag cacaacgtat tttccctgat tacaacgaca tggcagacaa
     1921 taaagcatat ttggagtcaa ttgcacgtaa ttttggatac cattacggac aagctaaaaa
     1981 agtgcgtgtt aacaccattt ctcaatcgcc aacaatgaca actgcaggaa gtggagtaaa
     2041 aggcttcaat gagtttatta attactcaga attaatgtcg ccacttggaa atgcaaaagc
     2101 agacgattgt gctaattatt gtattgcaat gttctcggat ttaacacgtt atgtgaccat
     2161 gcagaattta ttccacgatg gaggcttctc aagcactggt gttacagagg ctgtaatcaa
     2221 ccgttttact gatacgaatt tataaaagtt taaaataaga atatgattaa gttaacaagc
     2281 attatttcta tctctggtaa gccaggattg tataaagtag ttgctcaagc aaaaaatagt
     2341 gtaattgttg aatcgttgga agacaataaa aaaattcctg cgtatgcaac agaccgaatt
     2401 tctgcattag aagatattag catttatacg tacaacgaag aaaaaccgtt gaaagaaatt
     2461 tatgctgaca tctataaaaa agaaaatggt ggcgcaacta tttcacacaa agaagatgct
     2521 aaaaaattgc aagcatattt cttggaaatt cttccagaat acgaccaaga gcgtgtatat
     2581 gtttctgatt tgaaaaaatt atttcaatgg tataatttgt tgcacaaatc agggaattta
     2641 gtgttggaag aagaagtatc agaagaagca acagctaaaa aagcagaagc tccagctgaa
     2701 aagaaagaga aaaaagcagc tccagcagct aaaaaaccag tagcagcaaa agctactcca
     2761 aaaccaaaaa ctgccgctgc aaaaccaaaa acaacggttc aaagaaaatc aggttcttga
     2821 ttttttgaaa tagaaaacaa tttaaagctc ctatcaaggg gcttttttta tacatttata
     2881 gcaaagattg acaatgacat atatcaaagg ttttaatgcc attcgtgctt tttcggtttt
     2941 gttggtagta ttaacgcacc tcgatttatc ggaatggatt cccaataccg attttgtgat
     3001 gatacgtgtt tggcctatga tgtcgggaga atttggggtg ttgatgtttt ttgtattaag
     3061 tggattttta attaccaaaa ttctcttgca cgaaaaagat aaatttggtt ttatcagtct
     3121 gaagaaattt tacatacgcc gttttttacg cttgtttcca cctcttattt tgttttttat
     3181 actcatgggt attgcttatt ggaatggttt gatgcaagac gctgtgttga gtgtaggata
     3241 tggaatattt tacgtttata actttgtacc caaatcactt tatttccctg aaatgggaca
     3301 tctttggtcg cttggagtgg aagaacaatt ttatatcctt tggccatttg ttttgacagt
     3361 tttcaaaaaa atgacttcta taaaatggat tgtctatagt atggttgtca tcagcggttt
     3421 tctaaccttt tggtttgcca acagtttact ttctcaaaat tattttactg aacggtggtt
     3481 cattccagca agtaccccca tattaattgg atgtttggca gctatcgttc atcacaccca
     3541 acctcaattg acgttggaaa tttgttcaag taaatggcta ccagttataa tggcattgtg
     3601 ctttgctttt acgttgtatg tgccatcaga attagtgaaa atagggtttt tatttcgtgc
     3661 cattgcagta gccttgttgt tattgtacct tgttcacaat caacaaaaaa aatggatgca
     3721 aattttggag tggaagccgt tggtttatgt tggtactatt tcttatggta tttatattta
     3781 tcaaggtttt ttcttgagta caggtggagg aagcgagatt tggttccaaa aatttcctca
     3841 aaatattagt ttagccgtat tgttggctat actatcgtat gaattgatgg aaaaaccaat
     3901 tttaaggtat aaaaagaagt ttgaaacaaa atagtcaaga atttatactt taaaacttga
     3961 atttaatggt tgattcgttt aattttggtt ataaaattag tttttttgga tgaaaaatcc
     4021 tttactttta gcggttttac cagctttgtt aatcggtttg ttgaatggtt atttacttga
     4081 gaaaaaaggg tataaccgtt ggcaatacac gttttatggc tttttagtga gttatatttt
     4141 tttagttaaa tcgattgctt attttttaga ataatgacag tttttaatag ttacattcaa
     4201 gcatttcagc aactcggatt ttatttatcc aatggtgcta tctggaaatt tatcattcct
     4261 tcagtggtgg ttactgtttt ctatggattg ttgtttttaa tgttttcttt tatactagca
     4321 ccacttaatt tgctcacttt tttaccttgg gtaggcgatt ttattcaaaa tacttatgat
     4381 tggttgtgga actcatcgtt atccgttgtt ttttatgccc aatcgtttac cgttcttacg
     4441 cttttatcac cgtttatgag cctattgtcg tctaaatgcg atgaaatgac aacgggtttt
     4501 gtggatgaat attcaactgc tcgtttgatt agcgatttat ggcgtgccgt gaagctaaac
     4561 attcgtttgt ttattttgta tttgattacc gcagcagtga ttttatggtt gattcagccg
     4621 ttgttgggcg agaccgttgc aaaaggtttg agctatttat tatcgtctta tttatttggt
     4681 ttttcgttgt ttgattacaa ttttgaacgc tatcaaatga atatttccac aacattcaaa
     4741 aaaggaaata ccaattggat tgttttatta atcggaggaa cgttgtttca gttcatttat
     4801 gtcattccaa ttttaggaac tctctttgca ccgtttttgg ttacgattat caccacacac
     4861 gtttttatca ccacaaagga tagttggata agataaaaaa aaagaggctg tttgaaatat
     4921 tcgaacaacc tcttaaaagt attttttttc tcaattttta aaatgcttcg ttagcatctt
     4981 ttggagaatt gtattcaatt aatgttttaa atgctttctt taagcgttca attaacggat
     5041 catctaccac ataaaatgga gcagtgtatt tgttgaagtt ttgaccacta atagaaacag
     5101 catttttacc attacctaca gaattatcac acttaaccgt taacattccg tctccttgaa
     5161 taaagaaatc tttagagtaa atattgctca tagattttac tttgttcaaa tcgatggttg
     5221 taacacaaac atccgaagac aaaacttttt tgtatgaaaa aagcccatcg ttatacgata
     5281 caaactctct tcctccagtt aaatgttctt ttaacttacc agcaatccat tccattgttt
     5341 cttctttggt aggttttttt tcagtttgtg ccatcgtgtt taactgtgca acaaaaaaca
     5401 caaacattac agataaaatt aatttaaaat tcttcatttt cttttttttc aaatttataa
     5461 aaaagtattg aatttaagcc ataaatactt gttttttaat gtaacaaaaa gatgttagct
     5521 aacttttatt acataatttg cgtctatcaa aacattgcca ctactatcgt atttagcaaa
     5581 ttgaaaacca ttttgaatac aaaaagcacc gtgttctcct tgtttattca acgcaataaa
     5641 acctacttga atatcttcta attttttgtt gcgcaatttt accaacttaa tgattcggtt
     5701 taccgcttct tcacatgctt tttgtggtga aagaccttgg cgcatgagtt ctacaaccaa
     5761 atggcttcca acggtgcgaa ttacttcctc tccgtgtcca gttgctgttg ctgcaccaac
     5821 ttcgttgtcc acaaacaatc ctgctccaat gatgggcgaa tcgccaacac gaccgtgcat
     5881 tttaaacgcc attccactgg tggtgcaagc tcccgataaa tttccatgcg catccagtgc
     5941 aatcattcca atggtatcgt ggttttcaat gttggctaca ggtttgtact cgctattttt
     6001 caaccattct ttccattctt tttctgacgc ttcaaccaat aaattttctt ttttaaatcc
     6061 ttgcgacaga gcaaaatcaa gcgcaccttc acccactaaa aatacatgag gcgttttttc
     6121 catcaccgca cgggcaacag aaattggatg tttgatgtgc tccaaacaag caactgctcc
     6181 aatgtttgaa aattcatcca taatacaagc atccaaggtt acacgaccat ctctatcggg
     6241 ccttccgcca taaccaacac tgcgttcatt ggggtcggct tctggtacgt gcacaccttt
     6301 ttcaacagca tcgagtgcgc gaccgttgtt tttaagtact tcccaagctg cttcgtttgc
     6361 tggaatacca aagcgccaag tggataaaac aattggtttg tttatcgttt tctttttggg
     6421 ttttgtttca ggatgttctg tagcaaaact ttgcaaaggt tttaatccca ttgcaacaga
     6481 tgcaagggca gaaagtttta aaaaattacg tctatcggtc atacttctat tgtaagcaat
     6541 gattaatatc aaagcctaaa gtacaaaata aaaccaagta acgttctgaa acgcgtctat
     6601 tttgttgctg taaaaaacag aatttagtgg tgtgtttctt cttttggagc ccattttgaa
     6661 gccaacattc caccggcaag cgccaacaca atcacgccca acgacaacca atcgggaata
     6721 tcaacatggt gaacaatcaa cattttaacc ccaacaaatg ccaagatgac aattaaactg
     6781 tagctgatgt atttgaattt atccaacatg cctacgatta agaaatacat ggaacgcaag
     6841 cccattacag ctaaaatatt ggagctaaac acaatgaatg gatcggcggt gatggctaaa
     6901 attgctggaa tactgtcgat ggcaaaaaat aagtcggtta actcaatgat gattaacgcc
     6961 acaaacaaag gtgttgcaaa ttttaatttt ccacgtttca caaaaaactt accaccataa
     7021 accgagcgag aaacaggata tactttctta atccacttgt ataacttgga atcttttggg
     7081 tttttaggtt tatcgtgact aatgagcatt ttgattgctg taaaaattaa aaacgctcca
     7141 aacacataga taatccaatc aaatttggtg ataagtgcca ctccaaaaat tatcatcaat
     7201 gcacggaaaa caatggcacc caacactcca taaaacaaaa cttcgtgttg gtattttttt
     7261 gcaatttgaa acgaagaaaa tatcaaggca attaaaaaga tgttgtccac actcaatgaa
     7321 agctcaatca aataaccggt gatgtatttc acaacagcat tggtaggcgt taaattagtg
     7381 gggttatcaa tccatttgtt ctcaaaagct aaataaataa cacccgaaaa taagaccgca
     7441 acagttaccc aaacaatggt ccaaatggtt gcttcttttg ttttgatttc gtgcggagaa
     7501 cggttaaata cgcccaagtc aagagccaaa gcaactacga taaatgcgat aaaaataagc
     7561 caaagtgtca tgtgaaaata gaattattgt tttttaaaaa agaatttaac gattacaggt
     7621 gttgttacca ctaaaacaat tccaagaatg ataaattcca aattgtgttt cacccaagga
     7681 atgtcaccca aaaggtaacc aagcgttgtt aagctaccaa cccataaaac agcaccaatt
     7741 acgttgtaaa ttgcaaattt ttgaacattc atgttaatgg ttcctgcaac tatgggtgca
     7801 aaagtgcgaa tgatgggtaa aaatcgagct acagcaatag taaaaccacc ttttttatcg
     7861 taaaaatcgc gggctgtttg caaatgtttt tgttttaaga accaagtgtc gtgtttacgg
     7921 tcaattaaaa atttgaattt attgccaaac caatacccaa aaaagttacc caaaacggtt
     7981 gaaagcacaa ataacgccat ccaaaacggc aaattgtaaa gcggattttc aaaagggtag
     8041 tgggcttcgc taacagatgc caaaacaatt ccagaaataa aaagaagcgg atcgcccggc
     8101 aaaacaaagc caaaaaacac ccctgtttcg ataaataaaa tggctaaaac taaatacaaa
     8161 ccaccgttgt gcatgatcca gtcggggttc attaaatttt caaaaaacga taagattaca
     8221 tccatttaag ttgttggagt tatgtgttga aattggagag atttatgtgc accttaaaag
     8281 tcaaacaatt cactaaaaat gctttttttc tttttgtgat ggtagtctga atctttcgaa
     8341 tactgctttt tatcgtgttg ttggtatgtt ggtatgttgg atgttgcacc atttgaacgt
     8401 tcaatgattt tatccaattc acctctgtct agccatacgc cacggcagtc gggacaataa
     8461 tcgatttcaa taccgcttct ttccgacatt actaaattga cattacagtt tggacatttc
     8521 atattcaact tttttgtttg ttaattcaca aaaataggct tgaatacgct agtttaacaa
     8581 aaaattaaga aaaattggga aaataattag ggcgcttgaa acaattgctg ggtgtaatct
     8641 agcaaatcaa ctgtgccaat ttttccatga ccgggaacta cgtattttac ttttggaaaa
     8701 acagttttta ctttttgaat ggttgtgggc caatcagcaa cagaagcatc ttctaaattt
     8761 cctttgccag cattgagctc ttttaccaaa catccaccaa aaagcacttt ttctgaaggg
     8821 aaatatccta ctacgttatc caacgtgtga ccagcaccaa aatagttgac tgttacttct
     8881 tttttgccca accaaaacac tagtgaatcg ttgaacgaat attggggaat gttggatttg
     8941 ttttttgttg caaaagcaat ggttttacta gaagcatagg atggaatttg atggtgatga
     9001 aaggcggtga gtccccccaa acaatcggcg tgaaaatgtg ttggaataat cgctttaatt
     9061 tggctgtgca aggtgttggt tatccactga atcaattctt cggaactggc actatcggca
     9121 ggagtatcaa aaataacaga tgtatgctta ttggtaacaa tcaaaccgtt gcaaggcacg
     9181 ttgccaaaat cgttggtttg caagtaggag gtgtgcacat aggtgtgttt tgtcagttgt
     9241 tgaatgacca aactctttga ttgatatacc acttttgttt tgggtgctcg tgctaacgaa
     9301 aggtgggagg ctataattaa aaataataga attactagag atttcatgtg ttctttttta
     9361 acgataaagt ttactattct caacaaactg gttcatatat tgttgccaac gtttatcttg
     9421 atgcaacggt aagagggttt catcagaaat aaaaaaatcg ttaccaccta gtttgaattt
     9481 atttgccaat aagtctagct gttcaaatgc tgcttcaatt tttcccgttt gagcaaagat
     9541 agaaacagct gccagtcgtt cgttgtgtgt tcctctaact acgtttaatt caaacgcttt
     9601 ttcgtataaa ttacttgctt tttttaaatt tccttgttct ttgtagctgg ttgcttgttt
     9661 aattatttgg ttgaaacaag ccctattttt aacatctaca atgtctttat caaattccat
     9721 tctttcatcg gaagaaaatg atgtgttttt aaaccttttt gcaataaaat cagcatactg
     9781 aatggttgtt tcatcgtagg gtaattgtgc taatttatac aaaactgaaa ttgcatcttt
     9841 gggagaaaat tcgttgtttg actttgaaaa ttgagtcatt tgagtgtaaa attgaaccat
     9901 ttgttcacca aaagattgtg ttgaatcttc agccattttt attaaatcac tttccaaaag
     9961 catcaatttt gaagatattt ttagataagc atcgtttatt gaaggcacaa acgttgtcat
    10021 gtggttgcta ttatctaatt gaacagattg ccagtaccaa tttttaggtg ttaagctgtt
    10081 gaaaatagca tctaattgtt gaactccagg aagaaacatt ttttcaacct ttccctctgt
    10141 gccaattgaa gtgtaaataa acttgtgatt ggaaggattg tttttcaaat aatttgccgc
    10201 ttgacggatg atttgctcgt cacatacaag taggttgggg cttatggcaa ttgttgcatc
    10261 aaaaagtgat tgatctttaa aaagttcgtg caaaacatag gtaccaccta atgaatgtcc
    10321 gatgcctaaa cgaaaatcag aagttgaata catggaatct atagtaggaa taacttcttt
    10381 ggctaaaaaa cgcgtaaaac gttgcgctcc gatggtatca ttttgtagat caccacaagt
    10441 tggtacaaat tcgtcccatc tattttgggt gtgaatagca actactatca atggaattcc
    10501 ttgttgagat tgtgcataat aatcaataat acctttggtg attgtaaaat aaggttcaaa
    10561 ttgaccatcc aacaaataag ccacatgaaa tggagccaca gttttttgct tttcgtatgc
    10621 ttcgggtaga aaaattgaaa ttttacgctc acctccaaat acagttgatt gaatggtatg
    10681 atcaattgtt gtagtttgtg ccgaaacatt ccaaacaata atagaaatga gtagcgtaaa
    10741 accaattttt atcataaaat tattttttat gaagatagca aaaaaatgaa aatgttgaaa
    10801 atgacttgat tttttttctt tacaacgcca cccattccat aataaaactc catgcccacg
    10861 aagtaaaaaa tcctacaacg atactaaccc caatgatgag gttgattaat ttttcgttga
    10921 gatgaaattg ttcggcaatt aaacttcctg tcaccaaggt tggcatggct gcttcaaaca
    10981 cactgatgcg ggcaatttgt ccgtgaacgc caatcaacaa agcggcaatg aataccaata
    11041 aaggagcaag aataagtttg taaaaagtgg ccgtaagaat ttgattgatt tgttttctcc
    11101 atcctttgaa tttgagctgt agcccaaccg aaaacaaagc cagcggacca accgttgcca
    11161 ctaatttatc gaaaaatggt tcggcaggcg ttaaatccac aaaaaatgaa agtgcaagtg
    11221 ccgaaatgca accaataaga ggagggaagc tcaccaatcg ttttagaatg cttttggctt
    11281 taatgccttc ggtttctgaa cgattgcctt tgatggcgca aataattccc acggtagaaa
    11341 gcagtaaaaa caacacttga tcgcaaatga tggcaatgct taaatcctgt tcggtgtagt
    11401 aagccataat gagtggaaaa ccaataaatg aggtattgct a
//
LOCUS       DAOQVQ010000025        10683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_6954_length_10683_cov_4.242755, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000025 DAOQVQ010000000
VERSION     DAOQVQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6954_length_10683_cov_4.242755"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKN22_01540"
     CDS             complement(<1..662)
                     /locus_tag="PKN22_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41416.1"
                     /translation="MKKTIAYFLSVGIVCSAMVACTTKNEENTKNTNPLEKKVDDDDP
                     SLPFDQRLKQYVERKLNTRPGENYSLKIYEEHLNDDDKKDMVITVNRLEDAYKSAEEK
                     NLVNKSEVLGYFGNHNYFFYWSSKTDKFTPPFIIASTPQRELKISFENISSESHKDIV
                     VDYAIRNSQFRKIYLMMDDLPFYSFQWKLYDGWGTDKVEAYCFEYGKGSYSNVKDIII
                     NFA"
     gene            complement(691..1797)
                     /locus_tag="PKN22_01545"
     CDS             complement(691..1797)
                     /locus_tag="PKN22_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNS41417.1"
                     /translation="MIFSVIDVETTGGNPKSSKITEIAIYKHDGVNVIGEFESLVNPE
                     MPIPEFVSNLTGITDEMVANSPKFYEIAKEIIEFTKGTVFVAHNVSFDYGMIRSEYKR
                     LGYDFRLPHLCTVQTSRKLLPDMESYSLGKLTRSLGIALKGRHRAGGDALATAKLLTH
                     LMETDDVTKFIQEEIDVNELHPLLNMEEIEEIPNKPGIYKFFDENNQLIYIGKGKYIR
                     TQIEQHLKNSTQKKSAAWGKNIARVEFELTGNELVAAIIEHQLLQRHSTPYNKKSSKT
                     TKQNLFDEDLAVMDFNGESFFILRKGRTKFERSILYVENGQFVGFGYCPFHMNHQLPI
                     YWKRYIQTTTTTDDFIQHIIYTAIRKEKIDKIKI"
     gene            1956..2420
                     /locus_tag="PKN22_01550"
     CDS             1956..2420
                     /locus_tag="PKN22_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41418.1"
                     /translation="MTASIMIDLAYKHMKTLETEFKPLTEEGKLECLIYNVLICNFSA
                     QSRYDKGKMEVLNSDLFGILISDFRLYVDETEEEKIGELIESRFKNHFRDIVMILEGG
                     AFDPNDSFWYMYRKPLTFEAVGEIDPEMMAKFKPVLVKMSKAILEEADNLPD"
     gene            2442..4454
                     /locus_tag="PKN22_01555"
     CDS             2442..4454
                     /locus_tag="PKN22_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNS41419.1"
                     /translation="MKKVNALMQESSPYLLQHADNPVDWVPWSEAAFERARQENKLVL
                     VSIGYSSCHWCHVMEHECFEDEEVAALMNQHFINIKVDREERPDVDQVYMTAVQLMTQ
                     SGGWPLNCFTLPDGRPIYGGTYFPKEQWMSILNSLHYTFTNEKERTEEYATMLHDGIV
                     ASELIDTPQEKTTFSNEKLHEMVVRWKRQFDTMEGGDARAPKFPMPSNYQFLLQYGVQ
                     FSDEKTMQHVELTLDKMAMGGIYDHVGGGFTRYSTDMLWKVPHFEKMLYDNAQLISLY
                     SQAYKVFKKPLYARVVHQTIEWLQREMQDFDGVYHAAIDADSQGEEGLYYCWSLQEYK
                     EALHENYDFASDLYAFNERGHWEDGKYIPLRVLTDAELQKKYNLTPEALEQKIAQVND
                     ALLQTRKRRIRPAIDDKCLTAWNALLITGLSDAYAAFQNEEYMYLALKMVRWVLKYQL
                     KDDNSLWRTRKHNISSIDGFLEDYATVIQAFVRFYEVTGLEEYALKARDITEYVLVHF
                     QNKESKMFYFTPDSTKLIARKMDINDNVIPSSNSIMANNLLVLGHLFGKTEWIETAHQ
                     QLANIYDGMEQYGSMYSNWATALFKCTQPYFEVVFSGDNKLENSALLQNQYVPNVLTA
                     YDSSVIALGMNKESTTDLIFVCSENSGCLPPFDSVEEVINVVKKQA"
     gene            complement(4509..5918)
                     /locus_tag="PKN22_01560"
     CDS             complement(4509..5918)
                     /locus_tag="PKN22_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41420.1"
                     /translation="MKKLLLSISALLCAQLTFGQETLFQDNFESGSSNWTLNTGSAAN
                     QWIVNNSYTGFSGFINNTPNQPSGINGAPTSNYLHIHNTTVVALGVSNANYQTGVYAN
                     QDATMTSSISTVGKTGVTFSYWYLAVGAAGDSEGKVYYSTDNGSSWNLLATYSGVSAW
                     TQATHTLPAFDNQATLKFKFNWTDGDLGQDPAFSIDDVLITANGQADAISNLVISNSD
                     AWCQGTTKNINVSFDATGTYTSGNVFTAQLSDASGSFASPTAIGTLNSSTSGAQVIAA
                     TIPNNVNAGNGYRIRVVSTSPALISDTDNGTNLVVHPQPNVVLANFDAMCVYHNPITL
                     NQGSPTGGIYSGPSVVSGQFNPSTAGVGSWAITYTYTDGNGCGGQATKNITVSACAGI
                     DEVASNSLSVYPNPASDKLFIEGNGVKRVLIYDLLGNEVASFDAPQTYYNIHQLNAGA
                     YLVKVVAENTTKMIKIQVK"
     gene            6074..6721
                     /locus_tag="PKN22_01565"
     CDS             6074..6721
                     /locus_tag="PKN22_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41421.1"
                     /translation="MDLLNFIFRLGVVFAIFGFIWGLISLGIRILTMGRQKQTAEVYI
                     LKAVQYLLLVDVTFLICYDQSIKGLNYNSQLVFGTIVLLLYFVGKFQQRKNKQLLFSV
                     YTNGMPNLEPTFNAKAEIAVILMAIAAFVLFWFQPEFAINPISVWFKDSILDIESTPI
                     FGFIFKVIGFFFLLNIIMKVVNSILMLVSGKAFDKTDDSSNNNEDDHFDSFEEIK"
     gene            6726..7358
                     /locus_tag="PKN22_01570"
     CDS             6726..7358
                     /locus_tag="PKN22_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNS41422.1"
                     /translation="MNFIDILLLLPLGWGVYKGFKNGFIIEICVLLAVLVGIYTGVNF
                     SDGTANFLKNNWNIQSQYMPVIAFTATFLAVGALIFFGGKMLEKAVSVVHLSPLNKFL
                     GILFGTIKVTYILSVFLVMLDAYNEKTNFLKKETTDASLLYHPIKNLSTTTIPQIKES
                     TIYLNNLYKAEQDSTGLTTQDIVRAKEIADSLGIDANDAKELKRIHEEYH"
     gene            7476..10103
                     /locus_tag="PKN22_01575"
     CDS             7476..10103
                     /locus_tag="PKN22_01575"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNS41423.1"
                     /translation="MEIPKTYEPQSAEERWYQHWMNKGYFHSTPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVQKLRAEGIKK
                     SDLTRDEFLKHAFEWKDKHGGIILEQLKKLGCSCDWERTDFTMNPDYSDSVIDVFIDL
                     YQKGKIYRGVRMVNWDPEAKTALSDEEVIHKEVNSKLFHIRYKVAESDDEWLTIATTR
                     PESIFGDTAICINPNDERYTHLHGKHAIVPIVNRKVPIILDEYVEMDFGTGCLKVTPA
                     HDVNDYDLGIKHQLETIDIFNDNGTLNQHGLHYDGQDRFEVRKAIEKELHDKGYLVKT
                     EDHINKVGYSERTNAVIEPKLSLQWFLSMKDLAQPALDNVMNGNINFHPDKFKNTYKH
                     WMENVKDWCVSRQLWWGHRIPAWYFGTGAEDFVIAKTAEQAAELASKKANKTIAPTDL
                     RQDDDVLDTWFSSWLWPISVFKGLTQPGNEEIKYYYPTNDLVTAPEIMFFWVARMIIA
                     GYEYMGDLPFRNVYYTGIVRDKLGRKMSKSLGNSPDPIELINQYGADGVRMGILISSP
                     AGNDLPFDTSQCEQGRNFTNKIWNAFRLVSSWEVKEIDQPESSVRAIEWFSSRFNEAL
                     TEINDLFDKFKISEALMAIYKLVWDDFCSWYLEMIKPGYQHPIDAKTLEATKDFFESI
                     LKLIQPFTPFIAEELWHLIRERQAGDDIIVAQWPTVGTIDASTLANFKRAEEVIIGIR
                     NIRNTNNIANKVKVDLLIKKNADYDRSFDSVVVKMGNLSSLEYTAEKVNNANSFIVAN
                     NEYYVPFGDSIDVEAELAKMQEELKYAKGFLASVQNKLKNEKFVSGAPANVVEMERKK
                     EADALSKIAILEEKMANLG"
     gene            10122..10469
                     /locus_tag="PKN22_01580"
     CDS             10122..10469
                     /locus_tag="PKN22_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNS41424.1"
                     /translation="MSKELQDRTKKFSLSIIDLVERMEYSMTKKVVMNQLLRSATSVG
                     ANYRAANRARSDREFISKMNIVLEEADECCFWLEIIKEKQWQNVDDLLKEADELTAIC
                     VSILKKMKIKIQR"
BASE COUNT         3439 a         1866 c         2068 g         3310 t
ORIGIN      
        1 gcaaaattga tgatgatgtc ttttacgttg gaataagatc ctttgccgta ttcaaaacaa
       61 taggcttcca ctttgtctgt tccccaaccg tcatatagct tccattggaa agaataaaac
      121 ggtaaatcgt ccatcatcaa ataaattttt ctgaattgcg aattgcgaat ggcataatct
      181 accacgatgt ctttgtgcga ttctgaagat atgttttcaa aagaaatttt caactcgcgt
      241 tgtggagttg acgcaataat aaacggaggt gtgaatttat cggttttact gctccagtaa
      301 aaaaagtaat tgtgattacc aaaataaccc aatacttccg atttgtttac taaatttttc
      361 tcttcagccg atttataagc atcttctaaa cgattgacag tgattaccat gtcttttttg
      421 tcgtcatcgt ttaaatgttc ttcgtaaatt tttaagctgt aattttctcc cggacgcgtg
      481 tttaatttgc gttctacgta ttgtttcaaa cgttgatcaa acggaagaga cggatcgtcg
      541 tcatctactt ttttttctaa tggattggta ttttttgtgt tttcttcatt ttttgtggtg
      601 caagcaacca tggcactaca tacaattcca accgatagaa agtaagctat tgttttcttc
      661 atgaaacaaa gataattgtt ctcaatgaaa tcaaattttt attttatcaa ttttttcttt
      721 tcggattgcc gtatagatga tgtgttgaat gaagtcatct gtggtggtgg ttgtttgaat
      781 gtatcgtttc caatatatcg gcaattggtg gttcatgtga aatgggcaat agccaaatcc
      841 aacaaactga ccgttttcca catacaaaat agaacgttca aatttggtgc gtccttttct
      901 taaaataaaa aacgattcgc cattaaaatc cattactgcc aaatcttcgt caaacaaatt
      961 ttgcttggtt gttttgcttg attttttatt gtagggtgtg gaatggcgtt gcaatagttg
     1021 gtgttcaatt attgctgcaa ccaattcatt ccctgtaagt tcaaactcaa ctcgtgcaat
     1081 attcttgccc catgcagccg attttttttg agtgctattt ttaaggtgtt gctcaatttg
     1141 tgtgcggatg tatttgcctt ttccgatgta aattaattga ttgttttcat caaaaaactt
     1201 gtaaattcct ggtttgtttg gaatttcctc aatttcttcc atgttgagca aaggatgcaa
     1261 ttcgttgacg tcaatttctt cttgaataaa tttcgttaca tcgtcggttt ccattaagtg
     1321 agtcaacagt ttagccgttg ccaatgcatc acctcccgct ctatggcgac ctttaagcgc
     1381 aatacctagt gaacgtgtca acttccctaa actatacgat tccatgtcgg gcaagagctt
     1441 gcgactggtt tgcaccgtac acaaatgtgg caatcggaaa tcataaccca atcgcttgta
     1501 ttcactgcgt atcatgccgt aatcaaacga tacattgtgg gcaacaaaca ccgttccttt
     1561 ggtaaattca atgatttctt tggctatttc gtaaaattta ggcgagtttg ctaccatttc
     1621 atcggtaatt ccagtcaaat tggatacaaa ttctggaata ggcatttctg gattcaccaa
     1681 cgattcaaat tcgccaataa cgtttacacc atcgtgttta tagatagcaa tctccgtaat
     1741 cttggaagat tttggattgc caccagtggt ttcaacatct attaccgaaa aaatcattgg
     1801 gcaaaattag tgaattattt tattttcaat gagcaattta caccaaagaa attgcgccgt
     1861 tttctttttt tgatgtgcat aaaattcaga cgttgaagtc ttgttaaaag ctattttatc
     1921 cgtaaattgc accacttttt aattcaaaca acaagatgac agcatcaata atgatagatt
     1981 tggcatacaa gcacatgaaa acgcttgaaa cagagtttaa gccattaaca gaagaaggaa
     2041 agttagaatg cctaatttac aacgtgttaa tttgcaattt tagtgcgcaa tcgcgttatg
     2101 acaaaggaaa aatggaagta ttgaactccg atttgtttgg aattttaatc agcgatttta
     2161 gattatacgt tgatgaaacc gaagaagaaa aaattggaga attgattgaa agccgtttca
     2221 aaaaccattt tagagatatt gtgatgattt tggagggagg tgcgtttgac cccaatgatt
     2281 cgttttggta catgtacaga aaaccactga cttttgaggc ggtgggagaa attgaccccg
     2341 aaatgatggc gaagtttaag cctgttttag taaaaatgtc taaagcaatt ttagaagaag
     2401 ccgataattt acccgattaa ttccctgcaa atttacccta catgaaaaaa gttaatgcat
     2461 taatgcaaga aagttcgcct tatttacttc aacatgccga taatcctgtt gattgggtgc
     2521 cgtggagtga agcagcattt gaacgtgccc gccaagaaaa taaattggtg ttggtgagca
     2581 ttggttactc ttcttgccac tggtgccacg tgatggagca cgaatgtttt gaagacgaag
     2641 aagtggctgc attaatgaac caacatttta tcaatattaa agtggataga gaagaacgcc
     2701 cggatgtgga ccaagtgtac atgacggcgg ttcaacttat gacacaaagc ggtggttggc
     2761 cattgaattg ttttacattg cccgatggtc gcccaattta tggtggaacg tattttccaa
     2821 aagagcaatg gatgtctatt ttaaattcgt tgcattacac gtttaccaac gaaaaagagc
     2881 gtaccgaaga atatgcaacc atgcttcacg atggaatagt tgccagtgaa ttgattgata
     2941 ctccgcaaga aaaaacaaca ttttccaatg aaaaattgca cgaaatggtg gtgcgttgga
     3001 aacgacaatt tgatacaatg gaaggaggcg atgctcgtgc tcctaagttt cctatgccga
     3061 gcaattatca atttttattg caatacggtg ttcagttttc ggatgaaaaa acaatgcaac
     3121 atgtggagct aaccttggat aaaatggcaa tgggtggaat ttacgatcat gtgggaggtg
     3181 gttttacacg ctattctacc gatatgttgt ggaaagtgcc tcattttgaa aaaatgttgt
     3241 acgataatgc ccaattaatt tccttgtact cgcaagccta caaagtgttt aaaaaaccat
     3301 tgtatgcgcg tgttgtgcac caaactattg aatggttgca acgcgaaatg caagattttg
     3361 acggtgttta tcacgctgca attgatgccg atagtcaagg cgaggaagga ttgtactatt
     3421 gttggtcgct gcaagaatac aaagaagctt tgcacgaaaa ctacgatttt gcgtcggatt
     3481 tgtatgcatt taatgaaaga ggacattggg aagatggcaa atacattcct ttgcgtgttt
     3541 taaccgatgc cgaactgcaa aaaaaataca accttacgcc cgaagcgttg gaacaaaaaa
     3601 ttgcacaagt aaacgatgct ttgttgcaaa ccagaaaacg ccgcattcgc ccagcaattg
     3661 acgataaatg tttaactgcg tggaatgcct tgctaattac aggtttatcg gatgcctatg
     3721 ccgcatttca aaacgaagaa tacatgtatt tggctttgaa aatggtgcgt tgggtgttga
     3781 aataccaact aaaagacgat aattcattgt ggcgaacaag aaaacacaac atcagttcaa
     3841 ttgatgggtt tttggaagac tatgccaccg taattcaagc gtttgttcgt ttttacgaag
     3901 taacaggctt ggaagaatat gcactaaaag ctcgagatat aacagaatat gtgcttgttc
     3961 atttccaaaa caaggaaagc aaaatgtttt attttacgcc cgattctacc aagttgattg
     4021 cacgcaaaat ggacatcaac gacaatgtga ttccatcgag taattcaatc atggctaaca
     4081 atttattggt tttagggcat ttatttggta aaaccgaatg gatagaaaca gcacatcagc
     4141 aattggctaa tatttatgac ggtatggaac aatatggctc catgtattcc aattgggcaa
     4201 cagcattatt taaatgtact caaccctatt ttgaagtagt attttcgggc gataacaaac
     4261 tagaaaacag tgcacttctg caaaatcagt atgttccaaa tgtgctaaca gcctatgatt
     4321 cttcggtcat tgcattaggg atgaacaaag aaagcacaac agatttaatt tttgtttgct
     4381 ccgaaaacag cggatgtttg cctccgtttg atagtgttga agaagttatc aatgtagtga
     4441 agaaacaagc ataaaaaaac ctgtgttgaa ccaaatcagc acaggttttt tcaatattta
     4501 ggtatttgtt attttacctg aattttaatc attttagttg tgttttctgc aactactttc
     4561 actaaataag ctccagcatt caattggtga atattgtaat aagtttgagg agcatcaaaa
     4621 cttgctactt catttccaag taaatcataa attaaaacac gtttaacacc attcccttca
     4681 atgaataatt tatcagaagc tggattaggg taaaccgaca atgaattgga agctacttca
     4741 tcaattcctg cacatgcact tacagtaatg ttttttgttg cttgtccacc acaaccattt
     4801 ccatctgtat aggtataagt tattgcccaa gatccaacac ctgcagttga agggttaaat
     4861 tgaccactca caacagacgg tccagaatat atacctcccg ttggactacc ttgattcaac
     4921 gtgatagggt tgtgatatac acacattgca tcaaagttgg caagcacaac attcggttga
     4981 ggatgaacca ctaaattggt tccgttatct gtatcagaaa ttaacgctgg gctagtagat
     5041 acaacacgga tacggtagcc atttcctgca ttcacattgt ttgggatggt tgctgcaata
     5101 acttgtgctc ctgaagttga tgaatttaat gttccaatag cagttggtga agcaaaactt
     5161 cctgatgcat cagataattg agcggtaaaa acatttccag aagtataagt gcctgttgca
     5221 tcaaaagaaa cattgatatt ttttgttgtg ccttgacacc atgcatcaga atttgaaata
     5281 accaagttgc taattgcatc tgcttgacca ttggctgtaa tcaacacatc atcaattgaa
     5341 aatgcagggt cttgacctaa atcaccatct gtccaattga acttaaattt tagagttgct
     5401 tggttatcaa atgctggtaa cgtgtgagtt gcttgtgtcc acgcactaac acccgagtag
     5461 gttgcaagta aattccacga agagccatta tctgtagaat aataaacttt tccttcgcta
     5521 tctcctgctg caccaacagc caaataccaa tagctaaaag taacacccgt tttccctact
     5581 gttgaaatgc ttgaggtcat tgttgcatct tggtttgcat atacccctgt ttgataatta
     5641 gcattggaaa cacctaaagc cacaaccgtt gtattgtgaa tatgcaaata gttggaggtt
     5701 ggcgccccgt ttattcccga aggttgattg ggagtattgt taataaaacc tgaaaatcct
     5761 gtgtatgaat tattcacaat ccattgattt gctgcagaac cagtgtttaa agtccaattg
     5821 gatgagccac tttcaaaatt atcctgaaaa agagtttctt gaccaaaagt tagctgcgca
     5881 cacaacagtg ctgaaattga aagtaatagt tttttcataa tgtaaaattt atagtattag
     5941 taattaaatg ctaatatagt ttttttccat aaaataagct gctcaattgc aaaaaaaaat
     6001 tttcacacaa aatatttagt aactttaaca attgagacac acaacattgt tttgattttc
     6061 tcatttttgt agtatggatt tattaaattt tattttccgc ctaggcgtag tgtttgccat
     6121 ttttggtttt atctggggat tgattagctt gggaatacgt attctcacca tggggcgcca
     6181 aaaacaaacg gcagaagttt acatattaaa agcggtgcaa tatttattat tggtagatgt
     6241 aacattttta atttgttacg atcaatccat caaaggactc aactacaaca gtcagttggt
     6301 atttggcacc atcgttttgt tgctctattt tgttggcaag tttcaacagc gaaaaaacaa
     6361 gcaattgctt tttagtgttt ataccaatgg aatgcccaat ttggagccaa cttttaatgc
     6421 caaggcagaa atagctgtga ttttaatggc tattgcagca tttgtgttgt tttggtttca
     6481 gccagaattt gccatcaatc ctatttctgt ttggttcaaa gacagtattt tagacatcga
     6541 atcaacgcca atttttgggt ttatttttaa agtaataggt ttcttctttt tgctcaatat
     6601 cataatgaaa gtggtcaatt ctattttaat gttggtttct ggaaaggcat ttgacaaaac
     6661 agacgattca tccaacaaca acgaagacga tcattttgat agttttgagg agataaaata
     6721 gaaacatgaa ctttattgat attcttcttt tattgcccct cggttggggt gtttacaagg
     6781 gttttaaaaa cggctttatt attgaaattt gcgtattatt ggcagttttg gtgggaattt
     6841 ataccggtgt taatttttcg gatggaacgg ctaattttct taaaaacaat tggaacattc
     6901 aatcacaata catgcctgtt attgcattta cagctacttt tttggctgtt ggagctttaa
     6961 ttttttttgg cggaaaaatg ctcgaaaaag cagtgagtgt tgtacaccta tcgccactca
     7021 ataaatttct aggtattctc tttggaacca ttaaagtaac atatattctg agtgtttttt
     7081 tagtaatgtt ggacgcctat aatgaaaaaa ccaatttttt gaaaaaagaa actacggatg
     7141 catcactgtt ataccatcca attaaaaatt tgtcaaccac caccattcca caaatcaaag
     7201 aaagcaccat ctatttaaac aatttataca aagccgaaca agattctaca ggattgacaa
     7261 cccaagacat agtcagagca aaagaaattg ccgatagttt ggggattgat gccaacgatg
     7321 ccaaagagtt aaaacgcatt catgaggaat accactaaaa tgcatcctgt tttgggctta
     7381 ctcaccagtt gttgaatcaa attgtaaata taaatttccc cttgcacaac taccgaatat
     7441 cactaacttt gtaaaaaaat aacgaaattt agaatatgga aattccaaaa acatacgaac
     7501 cgcaaagcgc agaagaaaga tggtaccaac attggatgaa taaaggctat tttcactcaa
     7561 cacctgatga acgtgagcct tacaccattg taattccccc accaaacgtg acgggagttt
     7621 tacacatggg acacatgctc aacaatacca tccaagatgt gttgattcgt cgagcgcgta
     7681 tgttaggaaa aaatgcatgt tgggtacctg gaaccgacca cgcatctatt gcaacagaag
     7741 caaaagtggt acaaaaacta cgtgcagaag gaattaaaaa atcggattta acacgcgatg
     7801 aattcttaaa acacgctttt gagtggaagg acaaacacgg aggaattatt ttagagcaac
     7861 ttaaaaaatt aggttgttca tgcgattggg aacgtactga ttttacgatg aatcccgatt
     7921 attcagattc tgtaatcgat gtttttattg atttgtacca aaaaggaaaa atttaccgtg
     7981 gagttcgcat ggtgaactgg gatccggagg caaaaacagc actttctgac gaggaagtaa
     8041 tccacaaaga agtgaattct aaattattcc atattcgcta taaagtggcc gaatcagatg
     8101 atgaatggtt aaccattgca accacacgtc cagagtctat ttttggcgat actgcaattt
     8161 gtatcaatcc aaacgacgaa cgctatacgc atttgcacgg aaaacatgcc attgtgccaa
     8221 ttgttaaccg caaagtgcca attattttgg atgaatatgt ggaaatggat tttggtacag
     8281 gttgcttaaa agttacacca gcacacgatg taaacgacta tgatttgggt attaaacacc
     8341 aattagaaac cattgatatt tttaatgata atggaacttt aaatcaacac ggattgcact
     8401 acgatgggca agatcgtttt gaggtgcgca aagccataga aaaagaattg cacgataaag
     8461 gttatttggt aaaaactgaa gaccacatca acaaagttgg atattctgaa cgcaccaatg
     8521 cggtaattga accaaaatta tcattgcaat ggttcttgtc gatgaaagat ttggcacaac
     8581 cagctttgga caatgtcatg aacggaaata tcaattttca tcccgataag ttcaaaaata
     8641 cttacaaaca ctggatggaa aatgtaaaag attggtgtgt atcgcgtcaa ttgtggtggg
     8701 gacaccgcat tccagcatgg tattttggaa cgggagcaga agattttgtg attgcaaaaa
     8761 ctgccgaaca agcagctgaa ttggcatcta aaaaagccaa taaaacaatc gctccaactg
     8821 atttgcgtca ggacgatgat gtgttagata cttggttttc atcgtggttg tggccaattt
     8881 ctgttttcaa aggattgaca cagccgggca acgaagaaat taaatattac tatccaacca
     8941 acgatttggt aactgcgcca gaaattatgt ttttctgggt ggctagaatg attattgctg
     9001 gttacgaata catgggcgat ttgccattta gaaacgttta ctataccgga attgttcgcg
     9061 ataaattggg tagaaaaatg tctaaatctt taggaaactc acccgaccca attgaattaa
     9121 tcaaccaata tggtgctgat ggtgtgcgca tggggatttt aatttcatcg cctgcaggaa
     9181 acgatttgcc gtttgataca tcgcaatgcg agcaaggtag aaactttacc aataaaattt
     9241 ggaatgcctt ccgtttggtg agcagttggg aagtgaaaga aatagaccaa cccgaatcat
     9301 ctgttcgagc aattgaatgg ttttcatccc gttttaatga ggcattaaca gaaatcaacg
     9361 atttatttga taagtttaaa atttctgagg cgttgatggc tatttataaa ttggtttggg
     9421 acgatttctg ttcgtggtat ttggaaatga tcaaaccggg ctaccaacat ccaattgatg
     9481 ctaaaacatt ggaagcaact aaagatttct ttgaaagcat cttgaaattg attcagccgt
     9541 ttacaccatt tattgccgaa gaattgtggc atttaattcg tgaacgccaa gccggagacg
     9601 atattattgt tgcgcaatgg ccaactgtag gcacaattga tgctagcact ttggctaatt
     9661 ttaaacgtgc cgaagaagta attattggta ttagaaatat tcgcaacacc aacaacattg
     9721 ctaataaagt aaaagttgat ttgttgatta aaaagaatgc tgattacgat agaagctttg
     9781 attcggtagt tgtaaaaatg gggaatttaa gctcgttgga atataccgca gaaaaagtta
     9841 acaatgccaa tagtttcatt gttgccaaca acgaatatta tgttcctttt ggcgattcta
     9901 ttgatgttga agccgaatta gcaaaaatgc aagaagagct aaaatatgca aaagggttct
     9961 tggcaagtgt tcaaaataaa ttgaaaaacg aaaaatttgt tagtggcgct cctgcaaatg
    10021 ttgttgaaat ggaacgtaaa aaagaagcgg atgcattgag caaaattgca attttggaag
    10081 aaaaaatggc taatttggga tgattaaata ggtttttgtt gatgagtaaa gagttgcaag
    10141 ataggacaaa gaaattctcg ctttcaatta ttgacttagt tgaacgaatg gaatattcaa
    10201 tgaccaaaaa agtagtaatg aatcagctat tgagaagcgc tacttcagta ggtgcaaact
    10261 atagggcagc aaacagagca agaagtgaca gagaatttat ttcaaaaatg aacattgttt
    10321 tagaagaggc agatgaatgt tgcttttggt tagaaataat aaaagaaaaa caatggcaga
    10381 atgttgacga tctactaaaa gaagcagatg aattaactgc aatttgtgta tcaattttga
    10441 aaaaaatgaa aattaaaatc cagcgctaac ttaaaatttc atccttcaaa tttcatcctt
    10501 catccttcaa atttcaaaac ttcatccttc aaatttcatc cttctaattt caaacttcaa
    10561 acttcaccct tctaatttca cccttcaaac ttcacccttc taatttcacc cttcaaattt
    10621 caaaacttac cccttgactt ctcaaacaac atataaaaca gtaaaagaac aatccgaagg
    10681 act
//
LOCUS       DAOQVQ010000026        10353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7231_length_10353_cov_3.829579, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000026 DAOQVQ010000000
VERSION     DAOQVQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7231_length_10353_cov_3.829579"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..726
                     /locus_tag="PKN22_01585"
     CDS             82..726
                     /locus_tag="PKN22_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS41425.1"
                     /translation="MKAQMFIDIHSHQATQKFHTVINADLNPTDNWCSYGVHPWYVEQ
                     TTVTSLQKLEQLIQQPNCVAVGECGLDKICNTAWDKQMHWFEQQIQLSEKHQKPLLIH
                     CVKAIQEIIVLKRQYQPQQPWIFHGFRKTTTLQQVLNEGFYISIGAAVMSDEKLQSIV
                     PEIPINRLFLETDDQPISIVSIYEKVAQIRGVSVEELEQQLLENYQRVFEKTKI"
     gene            750..1481
                     /locus_tag="PKN22_01590"
     CDS             750..1481
                     /locus_tag="PKN22_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNS41426.1"
                     /translation="MGDWLERSELLLGKEALEKIKKSHVLVVGLGGVGSFAAEFICRS
                     GVGKMTIIDGDVFDVTNKNRQLMAMDSTIGKNKAVVLGEKLKDINADLDLTIISEFVV
                     PERVWEILEETKPDFVMDCIDSVSPKIEWIAACLRMKIRIITHLGAGGKMDPFAVKIA
                     RLTATYNCKLAAHLKDRLRRRGIDTKRVRAVFSSELQRKDALKMTDGSNFKKSFYGTS
                     SYTPALFGLMGAAEVIKYLSGQEKF"
     gene            complement(1478..1810)
                     /locus_tag="PKN22_01595"
     CDS             complement(1478..1810)
                     /locus_tag="PKN22_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41427.1"
                     /translation="MKIVNRGFLIVSPKKPFFDWANNFEEEIYFNESDEVEPTVYLIE
                     EDFIESEHVIKSHYKKIFLNELSMITENPEDHPEISEQLFYEWFKVVDGTTVLDNLKS
                     DLKRFDID"
     gene            complement(1818..2450)
                     /locus_tag="PKN22_01600"
     CDS             complement(1818..2450)
                     /locus_tag="PKN22_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41428.1"
                     /translation="MKIWLYSFFFLALVACSSPNDQPVDLEEIMPKATADKQPKKVET
                     EQKQQFTTSLNLELLKENSIHWDSVSLNDELTIPERFAPKQVEKFDYWLSGKPITYLR
                     WSFKDSTKSMNAFLNWMNCFGKRCSMVEIGKKTNIQKTNLLILQNDTCIIQLHTLDAS
                     LNELKKWKKIYLLDKESKWNFILMQSKFGNVAWTNYVDEKEGIIAPTLTK"
     gene            complement(2492..4051)
                     /locus_tag="PKN22_01605"
     CDS             complement(2492..4051)
                     /locus_tag="PKN22_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNS41429.1"
                     /translation="MKYIILLASIVLFTSCKTKITEQEIIQKHLTLEETLQLMLSPEY
                     QQTLGIDEQGRNLLYAYYEQRNFKPLWTSKEGLNEIGTALHHLLQTPIQFGLSKKRFD
                     LNWSDSVALQNELAIVCGLTRSFKDLHYGMSDSTLEVLKPNRYVPLNSLDTLLDFSQK
                     KYAEKIIAWGPADTNYHTLANYLFSFVQQNDITSIPTTIKPENQDSTNARALTQQFLV
                     QHHYLDSLQQDDSLSFVRALRRFQEKNWVNPTGKIDNETVQALNETNLHKAQRIALEM
                     EKIRRKEKSPKRYFLINIPEYKLRLYNEDTLCSEHTIVVGKLENQTPELKSKLHTIVA
                     YPFWNVPYSIASKEILPAAQANPNYFDRNNMVLLRNKDTINPYKVKWGKIKKNSFPYK
                     VVQQPGPKNSLGVLKFEFHSKYDVYFHDTPSKNYFKKIAKTYSHGCIRTENPLELAKI
                     VLELDENPVTADSLDSLMLLPLKHHKIPIKNRIPIFIEYHTVVITPEKEVVLLRDIYF
                     RDDKYIYGLDL"
     gene            4171..5157
                     /locus_tag="PKN22_01610"
     CDS             4171..5157
                     /locus_tag="PKN22_01610"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS41430.1"
                     /translation="MKIAITGASGHIGGVLCRELIKEGHEVVALVRSSTKALKDLKLT
                     KVDGDVLQLDSLRQLMQGCDAVIHAAGAIKLGYKFNKKLNDINVVGTKNVLQIAQELN
                     IPKVVHFSSIHVFKQSPYEEELNETRVFVGDKSVFYDQTKRDGHLLALEAAKNGQHVV
                     VVCPTGVVGPIDFAPSKLGKAVIDIYNGKIPAVVKGGFDFVDVRDVVQGTLLALEKGR
                     SGETYILGGHYYSIKAFADMVLHVKNSKKKMAVLPFFMAILGVPFIKLWAMLTKQPPL
                     YDLVYMDILQDGNQKVSSNKAKNELGYQPRPLTETIADTINWFKETGKIKTK"
     gene            5154..5918
                     /locus_tag="PKN22_01615"
     CDS             5154..5918
                     /locus_tag="PKN22_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNS41431.1"
                     /translation="MSYEIFNYFTQAWIAIAVIVFFALLFITAPYGRHTKRSWGPLID
                     NRVGWVVMEIFVIAVLYFFIFTGRYASIQSTTNIVIIGLFSLHYFNRSLIFPFRLRTK
                     GKKMPLSIVLMGMTFNLVNGFLIGYFLGNFRVYGNDWLTSPEFIVGTVLFFIGLIINW
                     HSDTVLINLRKPGETGYKIPQGGLFRWVSCPNLLGEVLEWGGFAVLTWSLPGLAFFIW
                     TFANLVPRALSHHKWYRSHFGEDYPAKRKAVIPFLW"
     gene            complement(5966..>6481)
                     /locus_tag="PKN22_01620"
     CDS             complement(5966..>6481)
                     /locus_tag="PKN22_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HNS41432.1"
                     /translation="NPSRHGAGVTGVPFTDTKRLESECGITMHSAHTHEVSSVFLYKV
                     IAAFGGAEAFYSKFYINSPFPLAIVQKTKKGDWVNANYYDDVQLFATVKEYMIETLRQ
                     HFAMGMNRKEVLVLGKKNATFIKKINAEAHLFDKMSVLEHPRFIQQYKSKEFQLYVDK
                     YLNALFDVEMK"
     assembly_gap    6482..6491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6492..6669)
                     /locus_tag="PKN22_01625"
     CDS             complement(<6492..6669)
                     /locus_tag="PKN22_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HNS41433.1"
                     /translation="MKTIANRVIAFNQSLVYSNQLPEGFGVLNPYVDNPETLEVMKQF
                     YKKYYNDNNQRTFII"
     gene            complement(6757..7527)
                     /locus_tag="PKN22_01630"
     CDS             complement(6757..7527)
                     /locus_tag="PKN22_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41434.1"
                     /translation="MATDKKVTHVGGNEPQNTSTSGNRPDFTPTKEAKGRATQLRLIA
                     GILWALAIGAQIWAINLLFKQPVVMWLIITLIVVDLALVIVGSLLWKKSNRLDPASEK
                     NKLLFFMQSQLGLFAAIIAFLPLVIFILTNKNIDKKQKAILGSIAGAALVIAGITGYD
                     FNPPSVEEYTQQTHQVEDLNNGQNLVYWTKSGTKYHLSDSCSYINTDKTDEIFTGTVA
                     QARELKKITELCSLCQSRAEKANATIPESAGEALETKE"
     gene            complement(7543..8946)
                     /locus_tag="PKN22_01635"
     CDS             complement(7543..8946)
                     /locus_tag="PKN22_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNS41435.1"
                     /translation="MNSAEIIKEQQKKVFGEHSKVVLFAPCRINEGILQLSENERKNT
                     IQNFKASEASPLYFIPASGSGSRMFAFLYDFLTQPDKADLSAVERFMNHIQEFAFFQI
                     FPNDLKERIIENTADAEEIARYLLSEEFLNYGNFPKGLIPFHFNEPFVLNPFQEHVLQ
                     GTRLSNGRAKFHFTVQPEFEKNIKESISNLEGLTGKSYQVSYSVQSKESDSYAFDLKG
                     NTIDQNGTPFRRPAGHGALLDNLNQLDEELIFIKNIDNVQHFSYSNFSMDTWAILGGV
                     LQEFKNELRAIYNHPTKEALVAANKKYQFLSPTEINQLTDSEAIKKIVNRPVRVCGMV
                     RNEGQPGGGPFWIDDNGAISKQIVEKAQINIQGEQFRLMVKSTHFNPVMIALSTKDLE
                     GKKLNLLDYRDDSKYFIVEKNQKGQDIKFMELPGLWNGGMAFWNTLFIEISSETFSPV
                     KTILDLLDAQHQNKQCK"
     gene            9084..9749
                     /locus_tag="PKN22_01640"
     CDS             9084..9749
                     /locus_tag="PKN22_01640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS41436.1"
                     /translation="MAVNEQDKKEIKNLISQLKNNHSTIQIADFGAGSKKLNNERKIS
                     SILKISSSKGKYARFFYQLAAYYQPQQVLEFGTSLGIGTLHFAKGNPSAQITTVEACP
                     QTAQVAQQNFEQLQLKNINLVNTTFQDFLDNTTLLPYDVIFIDGHHDGVALSNYVDML
                     FPYMKDDTFVILDDIRWSNFMFESWQKLTQDNRFHVSIDLMRMGILLKRPQQRKEHFT
                     IRL"
     gene            9785..>10353
                     /locus_tag="PKN22_01645"
     CDS             9785..>10353
                     /locus_tag="PKN22_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNS41437.1"
                     /translation="MKKINIRDLSLEELKSQLVALGQQAFRAKQVYEWLWNKNAHTFE
                     EMSSLSKELRAKLEEHFFIDGIKVVDQQASSDKTIKCAFEIEQGKVVEGVLIPTSSRM
                     TACISCQVGCSLSCDFCATGKLKLLRNLSAGEIYDQVAYLKRLAEEKYNTPLTNIVYM
                     GMGEPMLNYKNVLRSIEMITAKEGLGMSPR"
BASE COUNT         3157 a         1793 c         1987 g         3406 t
ORIGIN      
        1 ggatggtcgc attattttct tgaaaaaaat ttattttaaa aattaaacaa gaaggacgtt
       61 tttcttaatt ttaaaggcaa gataaaagca caaatgttca tcgatatcca cagccatcaa
      121 gcaacgcaaa aatttcacac agtgattaat gcggatttaa atccaacaga taattggtgt
      181 tcgtatggtg tgcatccgtg gtatgtggaa caaactactg ttacttcatt gcaaaagtta
      241 gaacagttga tacaacaacc caactgtgtg gctgtaggtg aatgtggttt ggataaaatt
      301 tgcaatacag cgtgggataa gcaaatgcat tggtttgagc aacaaattca actttccgaa
      361 aaacaccaaa aaccgttgct tattcattgt gtgaaagcca ttcaagaaat aattgtccta
      421 aaacgccaat accaaccaca acaaccgtgg atttttcatg gttttagaaa aacaactact
      481 ttacaacaag tgttgaacga aggtttttat attagcattg gagctgcggt tatgtccgac
      541 gaaaaattgc aatcaatagt tccagaaata cccattaatc ggttgttttt agaaaccgac
      601 gatcaaccaa tttcaattgt ttcaatttat gaaaaggtgg cgcaaattag aggagtttcg
      661 gttgaagaat tggaacaaca actactagaa aactaccaac gagtttttga aaaaactaaa
      721 atctaactat ttttgaaaaa aaaaacaaag tgggagattg gttagaacgt tctgaattat
      781 tgttgggcaa ggaagccctt gaaaaaatta aaaaatcaca tgttttagtg gtcggattgg
      841 gcggtgtagg ctcgtttgca gccgaattta tttgccgttc gggagttgga aaaatgacca
      901 ttattgacgg agatgttttt gatgtcacca ataaaaatcg tcagttgatg gccatggatt
      961 ctaccattgg taaaaacaaa gcggttgttt tgggcgaaaa actcaaagac atcaatgctg
     1021 atttagattt aacaatcatt tcagaatttg ttgtacccga gcgtgtatgg gaaattttgg
     1081 aagaaaccaa acccgatttt gtgatggatt gcattgactc tgtaagtcca aaaattgaat
     1141 ggattgccgc ttgtttgcgc atgaaaatca gaattattac gcatttagga gcaggcggaa
     1201 aaatggatcc atttgctgtg aaaatagcaa gattaactgc tacttataat tgtaaattag
     1261 cagctcatct taaagataga ttgcgacgac gaggaattga taccaagcgc gtaagagccg
     1321 ttttttcaag tgaattgcaa cgaaaagatg ctttaaaaat gaccgatgga agtaacttta
     1381 aaaagtcatt ttacggaaca tcttcctaca cgccagcatt gtttggattg atgggagcag
     1441 cagaagtgat taaatacttg agcggtcaag aaaaatttta atcgatatca aaacgtttca
     1501 aatcggattt aagattatct aaaaccgtag ttccgtccac cactttaaac cattcgtaaa
     1561 atagttgttc gcttatttct ggatgatctt ctgggttttc ggtaatcatg cttaattcat
     1621 tcaaaaatat ttttttgtaa tgacttttaa tcacgtgctc cgactctata aaatcttctt
     1681 cgattagata aacggttggc tccacttcat ccgattcatt aaaataaatt tcttcttcaa
     1741 aattgttggc ccaatcaaaa aaaggttttt taggcgatac aattaaaaat ccacggttta
     1801 caattttcat ctttttacta tttagtgagg gtgggtgcaa ttattccttc tttctcatca
     1861 acgtaattgg tccatgcaac gttgccgaat ttactttgca ttaaaatgaa attccattta
     1921 ctttctttat ccaaaaggta gattttcttc cattttttta attcgttcaa gctagcatcc
     1981 aacgtgtgta attgaataat acaggtatcg ttttgcaaaa taagtagatt ggttttttga
     2041 atgtttgttt ttttgccaat ttccaccatc gaacaacgtt ttccaaagca attcatccaa
     2101 tttaaaaaag cattcatgga tttggtagaa tctttaaaac tccaacgcaa ataagtaatg
     2161 ggttttccgc tcaaccaata atcaaatttt tctacttgtt tgggggcaaa acgttcggga
     2221 atggttaatt catcgttcaa cgaaactgag tcccaatgaa tggaattttc ttttaataat
     2281 tcaaggttca acgaagttgt aaactgttgt ttttgttcgg tttcaacttt ttttggttgt
     2341 ttatcagcag ttgctttggg cattatttct tccaaatcca ccggttggtc gttgggtgaa
     2401 gagcaagcaa caagtgccaa aaagaagaag ctatacaacc aaattttcat tgctatatat
     2461 tttttacaaa gttgaataaa ttgcttggtt tttacaagtc caaaccataa atgtatttgt
     2521 cgtctctaaa ataaatatcg cggagcaaca ccacttcttt ttcaggagtg atgacaactg
     2581 tgtggtattc aataaagatt ggtattctat tcttaattgg aattttgtgg tgtttcaaag
     2641 gcaacaacat taaactatcc aaactgtcag cggttacagg gttttcgtcg agttctaaaa
     2701 caatttttgc taattccaac gggttttctg tacgtatgca tccgtgcgag taggtttttg
     2761 caattttttt gaaataattt ttagaagggg tatcgtgaaa atagacgtcg tatttactgt
     2821 gaaattcaaa tttcaatacc cccaacgagt tttttggtcc cggttgctgc actactttat
     2881 acggaaaact attttttttg atttttcccc actttacttt ataagggttt attgtgtctt
     2941 tgttgcgcaa cagcaccatg ttgtttcgat caaaataatt gggatttgct tgagcagcag
     3001 gtaaaatttc cttgctagca atggagtagg gcacgttcca aaacggataa gccacaatgg
     3061 tgtgtaattt ggatttcaat tcgggagttt gattttccaa tttcccaaca acaatggtgt
     3121 gttccgaaca caaggtatct tcgttataca aacgcaactt gtattctgga atattgatta
     3181 aaaaatagcg tttgggagac ttttcttttc gtctaatttt ttccatttca agggcaatgc
     3241 gttgtgcttt gtgaaggttg gtttcattca gtgcctgcac cgtttcgttg tcgatttttc
     3301 ccgtagggtt gacccaattt ttttcttgaa atcttcgcaa tgcccgcaca aaagaaagtg
     3361 aatcgtcttg ttggaggcta tccaaataat ggtgttgcac taaaaattgt tgagtaagtg
     3421 cacgggcgtt ggttgaatcc tgattttcgg gttttatggt ggttggaatg gaggtaatgt
     3481 cgttttgttg cacaaacgaa aacagatagt ttgccaatgt gtggtagttg gtatcggccg
     3541 gaccccaagc aatgattttc tcggcatatt ttttttgcga aaaatcgagc aatgtatcca
     3601 gcgaattcag tggtacgtag cgattaggtt ttaatacttc caacgtagaa tctgacatgc
     3661 cgtagtgcaa atctttaaaa cttcgggtca atccgcaaac aattgccaat tcattttgaa
     3721 gtgctacact atcgctccag tttaaatcaa atcgtttttt agatagccca aattgaatcg
     3781 gtgtttgcaa taaatgatgg agcgcagtgc caatttcatt caacccttct tttgaagtcc
     3841 acaggggttt aaaatttcgt tgttcgtaat aagcatacaa caaatttctt ccttgctcgt
     3901 ctatgcctaa tgtttgttgg tactcaggag aaagcattaa ttgcaatgtt tcttcgagtg
     3961 ttaagtgctt ttgaataatt tcttgctctg taatttttgt tttacagctt gtaaatagta
     4021 caatagaagc cagtaagatg atgtatttca ttatttaaaa atagtaatta aatttgatgt
     4081 gcattggtat ttcgggtaga aaataatcaa tcaccaacct tttgtcgctt ttgcattgat
     4141 aattaaatct ttattttact tttgcgttgt atgaaaatag caattacagg agcatctggc
     4201 catattggag gcgttttgtg ccgagaatta atcaaagaag ggcatgaagt ggtggcgttg
     4261 gtacgtagtt ctaccaaagc acttaaggat ttgaaactca ctaaagtaga tggagatgtg
     4321 ttgcagttgg attcacttcg tcaattgatg caagggtgcg atgctgtaat tcatgctgca
     4381 ggtgccataa aattgggtta taaattcaat aaaaaattaa acgatatcaa tgtggtgggc
     4441 actaaaaatg tgttgcaaat tgcccaagaa ttgaacattc caaaagtggt tcatttcagc
     4501 tccattcacg tttttaaaca aagtccctac gaagaagaat taaacgaaac acgtgttttt
     4561 gttggagata aatctgtttt ctatgatcaa acaaaaagag atgggcacct gttggctttg
     4621 gaagcagcaa aaaatggtca acatgttgtg gttgtttgtc ccacaggtgt tgtcggtcca
     4681 attgattttg ctccgtcaaa actcggtaag gctgtcatag atatttacaa cggaaaaatt
     4741 ccagctgttg ttaaaggtgg gtttgatttt gtagatgttc gcgatgtggt gcaaggaacg
     4801 ttattggcat tggagaaagg aagaagtgga gaaacctata ttttgggagg tcattattat
     4861 tccataaaag catttgccga catggtgttg catgttaaaa atagcaagaa aaaaatggct
     4921 gttttacctt tttttatggc aattttagga gttccattta taaaattatg ggcaatgtta
     4981 accaagcaac cacccttgta tgacctagtt tatatggata ttttgcaaga tggcaaccaa
     5041 aaagtaagct ccaataaagc aaaaaatgaa ttgggatatc aaccacgccc gttgacagaa
     5101 accattgctg atacaataaa ttggtttaaa gaaacgggga aaattaaaac aaaatgagtt
     5161 acgaaatttt caattatttc acgcaagcat ggattgcaat tgctgttatt gtgttttttg
     5221 cacttttatt tataacagca ccttatggtc gccacaccaa aagaagttgg ggacccttga
     5281 ttgacaaccg tgtgggttgg gtggttatgg aaatttttgt aattgcagtg ttgtactttt
     5341 tcatttttac agggcgttat gcttccattc aatccactac caatattgtt attattggtt
     5401 tgttttcctt gcattatttc aatcgcagtt tgattttccc atttcgtttg cgcaccaaag
     5461 gcaaaaaaat gccactttcc attgtattga tgggaatgac ttttaatttg gtaaatggtt
     5521 ttttaattgg ttatttctta gggaatttta gagtttatgg caatgattgg ttaaccagcc
     5581 ctgagtttat tgttggcacc gttttgtttt ttattgggtt aatcatcaat tggcattcgg
     5641 atacagtgtt aatcaattta cgaaaaccgg gcgaaacagg ctataaaatt ccgcaaggtg
     5701 gattatttcg ttgggtgagt tgcccgaatt tattgggcga agtattggaa tggggcggtt
     5761 ttgcagtttt aacgtggagt ttgccaggtt tggcattttt tatttggact tttgctaatt
     5821 tagtaccaag agctttgtcg caccacaaat ggtatcgttc tcattttgga gaagactatc
     5881 cagcaaaaag aaaagcagtt attccgtttt tgtggtaatt ccgttttttt ataagagatt
     5941 ctgattaatt tgaatgttga aatttttatt tcatttccac atcaaaaaga gcatttagat
     6001 atttgtcaac atacaattgg aattctttgg atttgtattg ttgaataaaa cgaggatgct
     6061 ccaaaacgga cattttgtca aataaatgtg cttcagcatt gatcttttta atgaaagtgg
     6121 catttttttt acccaaaaca agcacttctt tgcggttcat gcccatggca aaatgttggc
     6181 gcaatgtttc tatcatgtat tctttaacgg tagcaaaaag ctgcacgtca tcgtaataat
     6241 tggcatttac ccaatcgcct ttttttgttt tttgcactat tgccaacgga aaaggtgaat
     6301 tgatgtaaaa cttgctgtaa aacgcctccg ccccaccaaa agcggcaatt actttgtata
     6361 aaaataccga agaaacttcg tgcgtgtgtg ccgaatgcat ggtaatgcca cattcacttt
     6421 ctaagcgttt ggtatcggta aaaggtacgc ccgtaactcc agccccgtgc cgacttggat
     6481 tnnnnnnnnn ncaatgataa acgtgcgttg gttgttatcg ttgtaatatt ttttataaaa
     6541 ctgcttcatc acctccaagg tttctgggtt gtccacatac ggattcaata ctccaaatcc
     6601 ttcaggcaat tggtttgaat acaccaacga ttggttaaat gcaatgacgc ggttggcaat
     6661 tgttttcatg ggagggatta tttgatgaag tgacgacaag gaaagaggaa cattaataaa
     6721 atcatgttat caatgctcct cccccaaaat aaaaaatcac tccttcgttt ccaatgcttc
     6781 tcctgctgat tctggaatgg tagcatttgc tttttcagca cgcgattgac acaagctgca
     6841 caattctgtt atttttttca actcacgcgc ttgcgccact gttcctgtaa aaatttcatc
     6901 ggttttatcg gtgttgatgt aggaacacga atcggataaa tggtattttg tgcccgattt
     6961 tgtccaatac accaaattct gaccattatt caaatcttca acctgatgcg tttgttgggt
     7021 gtattcttca accgatggtg gattgaaatc gtatccagta attccagcaa ttaccaatgc
     7081 cgcacctgca atacttccca aaattgcttt ttgttttttg tcgatgtttt tattggttaa
     7141 aataaaaata acaaggggta aaaatgcaat gatggctgca aataaaccca attgactttg
     7201 catgaaaaat aacaatttat ttttttcaga tgcagggtct aaacggttgg attttttcca
     7261 taaaagtgaa ccaacaatta ccaaggctaa atccaccaca atcaacgtga tgattaacca
     7321 cattacaaca ggttgcttga acaataaatt gattgcccaa atttgagccc ctattgccaa
     7381 tgcccataaa ataccagcaa ttaggcgcaa ttgcgtagct cttccttttg cctctttggt
     7441 aggcgtgaaa tcaggtctat tacccgatgt tgaggtgttt tgtggttcat ttccacctac
     7501 gtgcgtaact tttttatcag tagccatata gttaaattta gattatttac attgtttgtt
     7561 ttggtgttga gcgtccaata aatccaaaat ggttttaact ggactaaaag tttccgaaga
     7621 tatttcaata aacagtgtgt tccaaaatgc cattccaccg ttccacaatc ccggtaactc
     7681 cataaattta atgtcttgcc ctttttgatt tttctcaaca ataaaatatt tagaatcgtc
     7741 gcggtaatcc aataaattca attttttacc ctccaaatct ttagttgaaa gcgcaatcat
     7801 cacagggtta aagtgcgtgc tttttaccat caatcgaaat tgctctcctt gaatgttaat
     7861 ttgggctttt tcaacaattt gtttggatat tgcaccgttg tcgtcaatcc aaaatggtcc
     7921 accacctggt tgcccttcgt ttcttaccat tccacacaca cgaaccggac gattcactat
     7981 ttttttaatt gcttcactat ctgtcaactg attaatttct gtaggagaca agaattggta
     8041 ttttttattt gctgcaacca acgcttcttt tgtcggatgg ttgtaaatgg cgcgcaattc
     8101 atttttaaat tcttgcaaaa caccacctaa aattgcccat gtatccattg aaaagttgga
     8161 atagctgaaa tgctgcacgt tatcaatgtt tttgatgaaa atcaattctt catctaactg
     8221 attgaggttg tccaacaaag caccatgacc agctggtctg cgaaaaggag tgccattttg
     8281 gtcgatcgtg ttgcccttca aatcaaaagc atacgaatcc gactctttgg attgaacaga
     8341 gtaagaaacc tgatacgatt ttcccgtcaa tccttctaaa ttggaaatac tttctttgat
     8401 gtttttctca aattcgggtt gcactgtaaa atgaaactta gctctaccgt tggatagacg
     8461 cgttccttgc aacacgtgct cttgaaaagg atttaacaca aagggctcat taaagtgaaa
     8521 agggattaat cctttgggaa aattaccgta atttaaaaat tcttcactca ataaataacg
     8581 ggcaatttct tcagcatcag ccgtgttttc aataattcgt tctttaagat cgtttggaaa
     8641 aatctgaaaa aacgcaaatt cttgaatgtg gttcataaat cgttccaccg cactcaaatc
     8701 tgctttgtcg ggttgtgtta agaaatcata caaaaacgca aacatgcgcg agcctgaacc
     8761 cgatgcagga atgaaatata gtggcgatgc ctcgctagct ttaaaatttt gaatggtatt
     8821 ttttcgttca ttttcggata attgtaaaat tccttcgttg atacgacaag gagcaaacaa
     8881 caccactttt gaatgttcgc caaatacttt tttttgttgt tcttttatta tttctgccga
     8941 attcatacgt aattttgaag tctattgatt taatttgtaa cgtgaatcta ttgttcgaat
     9001 atataaaata tcattggaaa gccaaaaaaa gacacggcac acattcacca tttatttatg
     9061 aattagtcga taaatgtcta caagtggctg taaatgaaca agataaaaaa gaaataaaaa
     9121 atttaatttc tcaattaaaa aacaatcatt ctaccattca aattgccgat tttggcgcag
     9181 gttctaaaaa attaaacaac gaacgaaaaa taagttccat tctcaaaatc agttcaagca
     9241 aaggaaaata tgcccgattt ttttaccaat tggcagctta ttaccagccc caacaagttt
     9301 tagaatttgg aacatcatta ggaattggca ccctacattt tgcaaaagga aatccatctg
     9361 ctcaaattac aactgtcgaa gcgtgtcctc aaaccgctca agtggcacaa caaaattttg
     9421 agcaattgca gttgaagaat atcaatttgg tgaatactac atttcaagat tttttggata
     9481 acactacgct gctgccttat gatgtaattt ttattgatgg acaccacgat ggagtggctt
     9541 tatcgaacta tgtcgatatg ttgtttccct acatgaaaga cgatacattt gttattctag
     9601 acgacattcg ctggagcaat tttatgtttg aatcgtggca aaaactaaca caagacaacc
     9661 gttttcatgt ttccattgat ttgatgcgca tgggaattct tttaaaacga ccgcaacaac
     9721 ggaaagaaca ttttaccatc cgtttgtagg tagaaaatac ctatctttgc acttcggtat
     9781 gagcatgaaa aaaatcaata ttcgcgattt atcgctggaa gaattaaaat ctcaattggt
     9841 agcattaggc caacaagcat tcagagcaaa gcaagtgtat gaatggttgt ggaataaaaa
     9901 tgcacatact tttgaagaaa tgtcttcttt gagcaaagaa ttacgcgcaa aattggaaga
     9961 acattttttc atcgatggca tcaaggtagt ggaccagcaa gcatcgtcgg ataaaaccat
    10021 caaatgtgct tttgaaattg aacaaggcaa ggtggtggaa ggtgttttaa ttcctacatc
    10081 ttctcgcatg acagcgtgta tttcttgcca agttggttgt agtttatcgt gcgatttttg
    10141 tgcaacaggt aagctaaaat tgttgcgtaa tttatcggct ggagaaattt atgaccaagt
    10201 tgcatacctt aaacgattgg cagaagaaaa atacaacaca cctttgacaa atattgtgta
    10261 catgggaatg ggcgagccaa tgttgaacta caaaaatgta ttgcgctcca tcgaaatgat
    10321 tactgcaaaa gaaggattgg ggatgtcgcc acg
//
LOCUS       DAOQVQ010000027        10310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7270_length_10310_cov_3.614627, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000027 DAOQVQ010000000
VERSION     DAOQVQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_10310_cov_3.614627"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(835..1089)
                     /locus_tag="PKN22_01650"
     CDS             complement(835..1089)
                     /locus_tag="PKN22_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41438.1"
                     /translation="MNVEKIEKEIRSPVKNVDIHIHKLRWDMRIEFSTIVNGRTFRVS
                     SQSFYVDENKDVKIENVDSIVYPMITHLSRQIFLTSDLTN"
     gene            complement(1118..1204)
                     /locus_tag="PKN22_01655"
     tRNA            complement(1118..1204)
                     /locus_tag="PKN22_01655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Ser,seq:gct)
     gene            complement(1209..1279)
                     /locus_tag="PKN22_01660"
     tRNA            complement(1209..1279)
                     /locus_tag="PKN22_01660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Pro,seq:tgg)
     gene            complement(1284..1356)
                     /locus_tag="PKN22_01665"
     tRNA            complement(1284..1356)
                     /locus_tag="PKN22_01665"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1321..1323),aa:Trp,seq:cca)
     gene            complement(1519..2010)
                     /locus_tag="PKN22_01670"
     CDS             complement(1519..2010)
                     /locus_tag="PKN22_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41439.1"
                     /translation="MLFLVSWNGVLAQSNEVNLVTGEENVNIGYVSNPNNLPECVVNV
                     ARSINITPVADLPQYSYISVVDVHVPKNEESCHFSYSIRYQYLEAYRTNNTPISNCST
                     YMKSVTCFNDITDKNVKLHLEFMTAGVFETIPVAQADDWTNGVHFWWIFIGYPIYMPV
                     VNT"
     gene            2492..2698
                     /locus_tag="PKN22_01675"
     CDS             2492..2698
                     /locus_tag="PKN22_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS41440.1"
                     /translation="MKRTNIKMIEGYISIDKACEMAGITRKTFYNWIEHGLPVTLDKQ
                     LNKQRLVNEVEWITYLKNLKESYK"
     gene            2698..3369
                     /locus_tag="PKN22_01680"
     CDS             2698..3369
                     /locus_tag="PKN22_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNS41441.1"
                     /translation="MIPFNENEEIIDNRSGIYAIINIKTKKFYIGSAMKFSKRKNLHL
                     HNLRKGKHHSKYLQNSYNKHGEDVFVFKVLEYVEDENNLLKVEQKYLDIYFHTGATYN
                     ICSTAGSQFGTKHTNETKIKLALIQKGKKHSECSKKKMSLAKQNIKNETKKKLSDAQL
                     KKPVIQLDINNNILTAFNSMTEAERITGIPSGNISRCCNEKCKSAGGYIWRIKDDINK
                     KNIFM"
     gene            3554..4957
                     /locus_tag="PKN22_01685"
     CDS             3554..4957
                     /locus_tag="PKN22_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41442.1"
                     /translation="MQATQTGFGRVFINTPGVNNIFKLHNFMKLGPLDRTESQVEKIY
                     SYNSKNEKINVAIVQTDESEITSSLSGDLPLSKRSVLETLRNKLFNIQIHYGNCSSPT
                     DFYTFESAMILKDVTLLSYNLSELNAIVPSQRAVVTESANLSIGNYYRITYPKFTNIF
                     TISGSSLLNGVVYIERSDCDESISNDVNLIGLLDKLGNTAFVYSLDNGKNWVESGTSG
                     VPHEGLPEEAKIIYANSRVYWSKINGGIARLYSFEASRILNGDTISESELYRTTIGGI
                     KGLTKTSRYIYGVGGTSDGLILKVDLTNEKVEIIEENTVFTTVGLTAIDVLDDNFFVV
                     GGLSGVYGIYHNGVLSSYVIDYPGATAETVQSIKVFDQNNWLIGTDFSVFRTSNRGNT
                     WQKLIQDGTYNNLTFYDNLYGYNVSSDAIYLTLDGGYSWKKVSSNISATVCSAFLSPE
                     DHNQLYICYSEEIKKTI"
     gene            complement(4995..5222)
                     /locus_tag="PKN22_01690"
     CDS             complement(4995..5222)
                     /locus_tag="PKN22_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41443.1"
                     /translation="MRKIWLHENDNVYPGIIVNETQDENIIVLKFDYVWRKVQTTVDK
                     VKDRTEDFSEFMFNFRRKFLTEVPIKWTLPK"
     gene            complement(5200..5646)
                     /locus_tag="PKN22_01695"
     CDS             complement(5200..5646)
                     /locus_tag="PKN22_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41444.1"
                     /translation="MKPIYLIIINFLLALLPVLTTTSVLNSTSQVAPIPTVEVMKVDE
                     MSLIYRRSYLITYKFSEYYNENIAKMSMQTMFAGAGISEFENGDYRGYIARYNTGNTF
                     AVLYKNNSSELITFKIIGRYGEVDIRVFESFLIKFTEGLNEKNLVT"
     gene            complement(5688..6584)
                     /locus_tag="PKN22_01700"
     CDS             complement(5688..6584)
                     /locus_tag="PKN22_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41445.1"
                     /translation="MNIVEIAEKYFKVNKQGNGTYRLSTPDGKFDTVVLWEATNSYHR
                     FSTGYSGGPFQFLKYIVGLNDDEIKEEYGESFVENALVKALTESHFSKSDTGYTLQDV
                     VFELGYNEYIKSRNISKETAEYFNLEVSDKDVIIPLYDRNKRRIGSLVRNHNPKVKGD
                     RYRTMLIGSNEKPCVWSFSELYKIQANSIIILVEGAWSVMRIHQVIKPLIPNILPIAT
                     LGTNIHDELKQYIYDYPIISILDDDEGGNHVHNILQQWKKEKINVKTYIPTFSIVSKV
                     SSSYVDDISDKMLIELFRKIIN"
     gene            complement(6601..7989)
                     /locus_tag="PKN22_01705"
     CDS             complement(6601..7989)
                     /locus_tag="PKN22_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNS41446.1"
                     /translation="MIIKFTQLLTKALTEKKPHYVTRIPICLLNETEKKYVEGLNKCL
                     QEGWMPEIETFREDYRFLPIQSEIPIDVMYENFTKMKRDEYIAEQTKEFVESNVQSGA
                     SPYQGMAEFQQKILDKTAIANPKIIDYASLPREAYVTNVYRSKYYLPHFEEISNGLQG
                     GDFVVVMAGTKGFKTTLLKALAHAAFMQGKENVVFCSQEQAVMSMAQQLDMHALSETH
                     STLRSGIDEQQMKRLKGFEKKLKNYKNKFYITPQVKSVKQLHEYITALGSDVKKVFID
                     GLNLMQGDFSDSYNSLQKVCAELKSYAIEHNLIIIAVTQSNREGYKSGTEVGAQHIAG
                     SFAIAMYADIMLALSTIEEINQKTKRKENHVYIRPILNRHGNLSRKIDMLVEFYDNGK
                     KCDQVFSLLPEDYDPQQTVISYATSDKQKGNFEAVTGLQWEKVVESIGAETAEKLMAM
                     NQENEKTELGAF"
     gene            complement(8088..>10310)
                     /locus_tag="PKN22_01710"
     CDS             complement(8088..>10310)
                     /locus_tag="PKN22_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41447.1"
                     /translation="LLGITQLHPIQHGLMFERFLAELRLEIGELPDVDVDISENDRHI
                     IQEYAKERWGFEPVGTVLTYAHSGAVRVIDRIYSNITGNELNDNLVADASDKGEESEE
                     FKKFISIQPWMLPMYNGLLGSISGYGAHACAVVPIDPEIPVPMEAWGKGIVVNYSESG
                     GNKTLQMLGLVKYDLLSSSNLATIKLLSEITGVRPPKVIEDNDPCFTVFEKEDLTGLF
                     QFDTRVGRNLINLMIKNGQKINSIRTLSDLTSLGRPGPLHENYHIAYTERTADITKHP
                     DFIKEVFKQTNGVLIYQEQVAELFARVAFKDYDKNAKEYGIVALKSLVPKNAKLAETE
                     KFKAGYEKMHKMFINGGEKYHGLDIFYLEELFASLVGFIRYGFNLSHSLSYANISAQE
                     AWYKYYYPQAFWSVILEGVSNSKEERGKLLRYIVDATLKSGLEFRPPHINTASLGYKL
                     LDDNKTIQCPITMMKGMGASNVNEILSNQPFNNLQEINEKTKLNKSIKLAMYNAGMLK
                     GLEGDLYSLGVCEIKEFKSVDEVQYEHSGLIEKIEVIGNVMFVTVNNVKYSILSEHSD
                     EIKAIAKKRKIKLSTSGKLLKIGTEILFNTVDSVIMNYKRVRYFEPEPEAMGYIEAIK
                     ASLGFAIPQSPSLYDYFKYAEGKEDKIVGYIVEIETRKTDKTTQHKLTLQNGRGYWFC
                     IEDRTAKGFIMASSKVKSLDEVKHLEVGDLIGLTLVMTKDKQGNIMTYNQIKEFKLLA
                     "
BASE COUNT         3443 a         1728 c         1430 g         3709 t
ORIGIN      
        1 acagctttct ttacttctac ttctgttgcc caagaatcaa catcaccaat aaaaatgtat
       61 tgcttagttc ttttactata aactttgtaa taataaagca tatttattcc ttaaataatt
      121 atctaactaa gtgccaacaa aacgtctcga acgtttattt tttgagcttc aatcaaacgt
      181 aattaccatt tatacgatgt tggcataagt aacggtgttt ggaatcgaac caaaaattca
      241 accttatgaa agttgacagg caccacgcct acaccgctaa gtttacaata ctcatttgtt
      301 ttcaccaatt gaaatttttt taattgctgt acgagtattt atattttaat cttttcttaa
      361 atgtttcttt tacaatatcc tattttttat ccacccacca agggacgcac gaatcgaacg
      421 tactatcgtt tcttaaaatg aaattgtttt taattgctgt aaggatattt tttttattta
      481 atatctacaa ttgtaaaaag tttcaacttt tcttctcaca atgtctaatc tatctgttaa
      541 aaaagagaat tcaatttgtc ctcgttcacc tctaatggac tttacttcaa tatacccaac
      601 ataaggggta ataaaagtaa ttgattcaac accttgcttt tgtaaaaaag taccaagaaa
      661 attttctatt gtcatattat atctcatttt cttatttata aattactatt aagattttaa
      721 cttaattctt aaatacaaca taacaagaaa tgcagagtta actgcttgta tttaatgttc
      781 aggcgaagat tcttgtttct tcagtaactt ataaagatat tatatcacaa tattttaatt
      841 tgtcaaatcg cttgttaaaa aaatttgtct acttaaatgt gtaatcattg ggtagacaat
      901 gctgtcaaca ttttctattt ttacatcttt attttcatct acataaaatg attgactaga
      961 aactctaaaa gttcgtccat ttacaatagt agaaaattcg atgcgcatat cccatctgag
     1021 tttatgaata tgaatatcaa cattttttac aggacttctt atttcttttt ctattttttc
     1081 tacattcata atttttatcc ttgcagtttt tacttagcag tagcagaggg atttgaaccc
     1141 ccgaacgcat ttctgcgcta acagtttagc aaactgccgc tttagaccac tcagccatgc
     1201 tactttagtc aggatagaac gaatcgaaca ttcgtctcag agatccaaac tccgtgtttt
     1261 accattaaac tatatcctga tagcggtgcg tcaagggatc gaaccttgct ctttcgattt
     1321 tggagatcga tgtgtcacct ttaacacttc cacaccatct ttaaaattac cagaagtata
     1381 aaaatactga atttcaccat caaccataaa agcataaata agtatcattt tgcctttatc
     1441 ttcacttaat ccatcaacag atttacatgg taaatattta ggcttttcta cttctggcaa
     1501 tataaacatt ctaatccttt acgtatttac tactggcatg taaattggat atccaataaa
     1561 tatccaccaa aagtgtacac cattagtcca atcgtccgct tgtgcaacag gaattgtttc
     1621 aaatacacct gctgtcataa attctaaatg caactttaca ttcttatcag taatgtcatt
     1681 gaagcatgta acgcttttca tgtatgttga acaatttgaa ataggcgtat tatttgttct
     1741 gtatgcttct aggtattggt atctaataga ataagagaaa tgacaacttt cttcattctt
     1801 tggcacatga acatcaacta ctgaaatata cgagtattgc ggtaaatcgg caacaggcgt
     1861 aatgttaatg cttcgtgcta catttactac acattcaggt aaattgttcg gattgcttac
     1921 gtatccaata ttaacatttt cttcgccagt taccaagtta acttcatttg attgagctaa
     1981 aacaccattc caactaacta aaaacaacac aaatactaat acataaattt taacaatatt
     2041 atttactttc ttttttattg cctttcatat tttataatat aaaaaaaatt attcagcaat
     2101 gttttcagca atgacagggt gactactgag acttgcactc agatattctt ccttcacagg
     2161 gaagcgcata aactgttctg ccatagtcac aaatgacggt gaaggatttg aaccttcgga
     2221 tggtaggttt aaatcatact aacttagcac aacgtattaa ttatatcata gattttttat
     2281 ttgtcaaatt tcttttcatt attgatttgc taaaaattaa taaaacttta gccatataat
     2341 attttttaaa aagcataagc atagttttaa atatttttac tatcttaaaa gcattattag
     2401 aaacttacta ttgacaaata cttatgaata ttatataata tatgtaacaa caaattataa
     2461 ttttgaatgc agaaagtagt tgtaggaaat aatgaaacga acaaatatta aaatgattga
     2521 aggttatatt tcaattgata aggcttgtga aatggcagga attacacgta aaacatttta
     2581 taattggatc gaacatggtt taccagttac gctggataaa caattaaata agcaaagatt
     2641 agtgaatgaa gttgaatgga ttacttactt gaaaaattta aaggaaagtt ataaataatg
     2701 ataccgttta atgagaatga agaaattatt gataatcgta gtggaattta tgcaataata
     2761 aatatcaaga ctaaaaagtt ctatattggt tctgctatga agtttagtaa acgaaaaaat
     2821 ttacatctac ataatttacg caaaggtaaa catcattcaa aatatttaca aaatagttat
     2881 aataaacatg gggaagacgt atttgttttt aaagttctag aatatgttga agatgaaaat
     2941 aatcttttaa aagttgaaca gaagtattta gatatatatt ttcatacagg cgcaacttat
     3001 aatatttgta gtactgctgg aagtcaattt ggaacaaaac atactaatga aacaaagata
     3061 aaattagcat taattcaaaa aggtaaaaaa cactctgaat gctcaaagaa aaaaatgtct
     3121 ttagcgaaac aaaatattaa aaatgaaact aagaaaaaat tatctgatgc tcagttaaag
     3181 aaacctgtaa ttcaacttga tattaataat aatatactta ctgcttttaa tagtatgaca
     3241 gaagctgaaa ggataacagg aattccaagt ggaaatataa gcagatgttg taatgaaaaa
     3301 tgtaaatctg ctggtggtta tatttggcga attaaagatg atataaataa aaagaatatt
     3361 tttatgtgaa catttttatt atttttttga ttatttaaat ttaccatcac aattttctca
     3421 tttatcaaat cacaaataaa aaagttgctt tcgccgcctt tgttaaaagg ataaggaaac
     3481 agtattgttt tgtcaaataa agtaaatgga caatcaatga aaaatatatt aagatcgtaa
     3541 gaaaggagac attatgcaag caacacagac agggtttgga agagttttta ttaatactcc
     3601 tggtgtaaat aacatattta aattacataa ttttatgaag ttaggaccgt tggatagaac
     3661 agaaagtcaa gttgagaaaa tttattcata taattctaaa aatgaaaaaa taaatgtagc
     3721 tattgttcaa acagatgaaa gtgaaattac ctcttcatta tctggcgact tgccgcttag
     3781 taagagaagt gtattagaaa cacttagaaa taaattattt aatattcaga ttcattatgg
     3841 taattgcagt tcaccaactg acttttatac atttgaatca gcgatgatat taaaagatgt
     3901 aactttatta tcttataatt tatcagaact aaatgcaatt gttccttcac aaagagcagt
     3961 agtaacagaa tcagcaaatt tatctattgg taattattat agaataactt atccaaaatt
     4021 tacaaacatt tttacaatat ctggtagctc attattaaat ggtgtagttt atattgaacg
     4081 ttctgattgc gatgaatcaa tttcaaatga tgttaattta ataggattat tagataaatt
     4141 aggcaataca gcttttgtat attcgttaga taatggtaaa aattgggttg agtcaggaac
     4201 aagcggtgta cctcatgaag gacttcctga agaagcaaaa attatatatg ctaatagtag
     4261 agtttattgg agcaaaatta atggtggaat cgctagatta tattcatttg aagcaagcag
     4321 gatattaaat ggcgatacaa tttcagaatc agagctatac agaacaacaa tcggtggcat
     4381 taagggatta acaaaaactt ctcgttatat ttatggcgtt ggtggaacaa gcgatggatt
     4441 aattttaaaa gttgatttaa ctaatgaaaa agttgaaata attgaagaaa atacagtgtt
     4501 tactacagtt ggattaacag caatcgacgt tcttgatgat aatttctttg ttgttggtgg
     4561 cttatcaggt gtgtatggca tttaccataa tggagtctta agctcttatg taattgatta
     4621 ccctggcgca acagcagaaa cagtacaaag tattaaagta tttgatcaaa ataactggct
     4681 tattgggaca gattttagtg tatttcgcac atcaaatcga ggtaatactt ggcaaaaact
     4741 aattcaagat ggcacatata ataatcttac attttacgat aatctctacg gttataatgt
     4801 atcttcagac gcaatttatt taactttaga tggtggttat agttggaaaa aagttagttc
     4861 taatatatct gctacagttt gtagtgcatt tctttctcct gaagaccata atcaattgta
     4921 tatttgctac tccgaagaaa ttaagaaaac aatttaaata aaaataacca tgcgcagcgc
     4981 atggttatta tatcttattt tggaagggtc cactttattg gaacttctgt aagaaatttt
     5041 cttctaaaat taaacatgaa ttcagaaaaa tcttctgttc tatcttttac cttgtccact
     5101 gtcgtttgga ctttacgcca aacatagtca aattttaaga ctataatatt ttcatcctgt
     5161 gtttcattca ctataatacc aggataaaca ttatcatttt catgtaacca aatttttctc
     5221 attaagtcct tctgtaaact ttattaaaaa tgattcaaat actcttatat caacttcacc
     5281 ataacgacca ataattttaa acgttatcaa ttcacttgaa ttatttttat ataatacagc
     5341 aaatgtattt cctgtattgt atctagcaat atatccacgg taatcgccgt tttcaaactc
     5401 gcttattccc gctcctgcaa acatcgtttg cattgacatt ttcgcaatat tttcattata
     5461 atattctgaa aatttataag taatgagata agatcttcta taaatcaaac tcatctcatc
     5521 aaccttcatc acttctactg ttgggatagg tgctacttgt gatgtactat ttaatactga
     5581 tgttgttgtt aaaacaggca gtaatgctaa taaaaaatta atgataatta aatatatcgg
     5641 tttcatgtta gtttttctcc tatgtgtcta caacctaaca ttgtagatta atttataatt
     5701 tttctgaata actctattaa cattttatca gaaatatcat ctacatatga agaactgact
     5761 tttgatacaa tactaaatgt aggaatataa gtttttacat taatcttttc tttcttccat
     5821 tgctgaagga tattatgaac atgatttcca ccttcatcat catcaagaat agaaattatt
     5881 ggataatcat aaatatattg ttttaattca tcatgaatat ttgttccaag cgttgctata
     5941 ggtaaaatat tgggtattaa tggttttatt acctgatgaa ttcgcataac tgaccatgcg
     6001 ccttcaacaa gaataataat actattcgct tgtattttat acaattctga aaaagaccaa
     6061 acacaaggtt tttcattaga accaataagc attgttctat atcgatcacc ctttactttt
     6121 gggttatgat ttctaactaa agaaccgatt cttctcttat ttctatcata aagtggtata
     6181 attacatctt tatcagaaac ttcaagatta aaatattctg ccgtttcttt actaatattc
     6241 ctactcttga tatattcatt atatcctaac tcaaaaacaa catcctgtaa agtgtaccct
     6301 gtgtcacttt tggaaaagtg actttcagtt aaggctttaa ctaacgcatt ctctacaaaa
     6361 gactcgccat actcttcttt aatttcgtca tcatttaacc ctacaatata ctttagaaac
     6421 tggaacggtc cacctgaata tccagttgaa aaacgatgat atgaatttgt agcttcccaa
     6481 agtaccactg tatcgaattt accatcaggt gttgacaaac gataagtgcc attcccttgt
     6541 ttattgactt tgaaatactt ttcagcaatt tctacaatat tcatatcatc atttccttac
     6601 ctagaaagcg ccaagttctg ttttttcatt ttcttgattc attgccatta atttttcagc
     6661 ggtttcagcc ccaatacttt caacaacttt ttcccattgt agccctgtca cagcttcaaa
     6721 attgcctttt tgtttatcac ttgttgcata actaattaca gtctgttgtg gatcgtaatc
     6781 ttctggtagt aatgaaaata cttggtcgca tttcttacca ttatcataaa attcaactaa
     6841 catatcaatt ttacgtgata aattaccatg acgatttaag attgggcgaa tgtatacgtg
     6901 attttctttt cttttagttt tttgattaat ttcttcaatt gtagaaagag ccaacataat
     6961 gtcagcgtac attgctattg caaaactacc tgcaatatgt tgcgctccaa cttccgttcc
     7021 actcttataa ccctcacgat tactttgtgt tacagcaata ataataagat tgtgttcaat
     7081 tgcgtatgat tttaattctg cacaaacctt ttgtaatgaa ttataactat ctgaaaagtc
     7141 accttgcata agatttaaac catcgataaa tactttttta acatcactac ctaaagctgt
     7201 aatatattcg tgtaactgct taacactctt tacttgtggg gtgatgtaaa atttattttt
     7261 gtaattcttt aatttctttt caaacccctt caaccttttc atctgttgtt cgtcaatacc
     7321 agatcgaagg gtagaatgtg tctcagaaag agcgtgcata tctagttgtt gtgccatact
     7381 cattacagct tgctcttgcg aacaaaaaac aacattttct ttaccttgca tgaatgctgc
     7441 atgagctaaa gctttaagta aagttgtttt gaaccccttt gttccagcca taacgactac
     7501 aaaatcacca ccttgcaacc cattactaat ttcttcaaaa tgaggtaaat aatattttga
     7561 acgataaaca ttagtaacat aagcttctct tggtaaactt gcgtaatcaa taatttttgg
     7621 gttggcaatt gctgtcttat ctagaatctt ctgttgaaat tctgccattc cttggtaagg
     7681 tgaagcacca ctttgtacat tgctttcaac aaattctttt gtctgttctg caatgtattc
     7741 atcacgtttc atcttagtga aattttcata catcacatcg attggaattt cgctttgaat
     7801 aggtaagaaa cggtaatctt cacgaaaggt ttcgatttca ggcatccaac cttcttgaag
     7861 acacttattc aatccttcta catatttttt ttcagtttcg tttaaaagac aaataggaat
     7921 tcttgtaaca taatgtggct ttttttccgt aagtgctttt gttaataact gtgtaaattt
     7981 aataatcatt ttcaatctcc tgtttgtttt gacggtagag acaattatag cataataact
     8041 taatctgtca actcaactaa taatttttaa gttattatgc tataatgtta agctaaaagt
     8101 ttaaattctt tgatttgatt ataggtcata atattacctt gcttgtcctt agtcatgacc
     8161 aacgttaacc caatcaaatc acctacttca agatgtttaa cttcatctag gcttttaacc
     8221 ttagaagaag ccatgataaa accttttgca gttctgtctt caatacagaa ccagtatcct
     8281 cgcccgtttt gcaatgttaa tttatgctgt gttgttttat cagtttttct agtctctatt
     8341 tcaacaatat aaccaacaat tttatcttct ttaccctctg cgtacttaaa gtagtcgtaa
     8401 aggcttggtg attgtggaat agcgaatcct aatgaagctt tgattgcttc aatatagccc
     8461 attgcttctg gttctggttc aaagtagcga actcttttgt agttcattat aacactatcg
     8521 acagtgttaa acaagatttc tgtaccaatt tttaacagtt taccagaggt agatagtttg
     8581 atttttctct ttttagctat tgctttaatt tcatcactat gttctgatag tattgaatat
     8641 ttaacattat taactgttac aaacattaca ttgccaatga cttcaatttt ttctatcaac
     8701 ccgctgtgtt catattgcac ttcatcaaca cttttaaatt cttttatttc acagacacca
     8761 agagaataca agtcaccttc caagccttta agcattccag cgttatacat tgctaacttg
     8821 attgacttat taagcttagt tttttcatta atttcttgta aattattgaa aggctgattt
     8881 gataaaattt catttacgtt actagcgccc attcctttca tcattgtaat aggacactga
     8941 attgttttat tatcatctaa taatttgtat cctaatgatg ctgtgttgat atgaggtggt
     9001 ctaaactcca agccactttt cagcgttgca tctacaatat aacgaagtag tttccctctt
     9061 tcttctttac tgttactcac accttctaga ataacactcc aaaaggcttg tggataatag
     9121 tatttatacc acgcctcttg tgcagaaata tttgcatagg ataaactatg actaagattg
     9181 aatccataac gaataaagcc aactagcgac gcaaaaagtt cttcaaggta aaagatatct
     9241 aatccatgat acttttcacc tccgttaata aacattttat gcatcttttc ataaccagct
     9301 ttaaactttt ctgtttccgc taattttgca tttttaggga ctagtgattt taaagcaaca
     9361 ataccatatt ctttagcatt cttatcataa tccttaaatg ctactcttgc gaataattct
     9421 gcaacttgtt cttgatagat tagaacacca ttagtttgtt taaagacttc tttaataaaa
     9481 tcaggatgct ttgtaatatc cgctgtacgt tcagtgtaag cgatatgata attttcatgt
     9541 aatggtcctg gtctacctaa acttgttaag tctgaaagag ttctaattga attaatcttt
     9601 tgaccatttt taatcatcaa attgattaaa ttacgcccaa ccctagtatc aaattgaaat
     9661 aatcctgtta aatcttcttt ttcaaaaaca gtgaagcaag gatcattatc ttcaattacc
     9721 tttggtggtc ttacacctgt aatttcactt aacaatttaa ttgttgctaa attagatgaa
     9781 cttaaaagat cgtattttac aagccctaac atttgtaaag tcttattacc acctgactct
     9841 gaataattaa ctacaattcc ctttccccat gcttccattg gtacaggtat ttcagggtca
     9901 atcggtacaa ctgcgcaagc atgtgcgccg tatccactaa ttgaaccaag taacccatta
     9961 tacattggta acatccaggg ttgaatagaa ataaactttt taaattcttc tgattcttcc
    10021 cctttatctg aagcatctgc aactaaatta tcatttaatt cattaccagt aatatttgaa
    10081 tagattcgat caattactct aactgctcct gaatgagcat aagttaatac tgtaccaaca
    10141 ggttcaaagc cccatctttc ttttgcatat tcttgaatga tatgccgatc attttcacta
    10201 atatcaacgt caacatcagg caattcacct atttctagcc gtaattcagc taaaaatcgt
    10261 tcaaacatta acccatgttg tatcggatgc aattgcgtaa ttcctaatag
//
LOCUS       DAOQVQ010000028        10204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7375_length_10204_cov_3.951424, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000028 DAOQVQ010000000
VERSION     DAOQVQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_10204_cov_3.951424"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKN22_01715"
     CDS             complement(<1..961)
                     /locus_tag="PKN22_01715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS41448.1"
                     /translation="MLYNILKFLVRIGIRIYYSEVKVRNGVNLQHNGPKIIISNHPNA
                     LMDAWLIGTISKEPIYYMTKATFFNNKLKMWFLRSLNMIPINRADEAKIEGVNNNSTL
                     EECYKILSEGKTLVVFPEGSSYMELLLRKLKSGTARIALEAEQRNGGKLNLKVIPIGL
                     MYTKGEKFRSSIMVNIGEGITVTDYLEEYKTNHSTAAKKLTQKFREILEQVLITTQNK
                     EQEALVTSLTEALQSRYLNNAKNVESELDLMKKIRDRIELYSIEKPQKIEKIQNLLAN
                     LNWNIKKMDIKNDFLDRGIRSRMFIRQLILSLLALILGFPIFLI"
     gene            1123..4374
                     /locus_tag="PKN22_01720"
     CDS             1123..4374
                     /locus_tag="PKN22_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41449.1"
                     /translation="MLLFLFKAEASHIMGGSITYECDGAGNYVFQLVFYRDCHGAHVN
                     TNNQTLKLWNHPTLSTISVAYVSTEDISPQGTQATGGPACFDCNDPNGNIGIGSIERI
                     TYRSAPISISGVPPAQGWVVTFDDFSRNGGITNLQTPLNYGITLTAKIFNVNYQNNTC
                     NDNSVQFKQMPHFVGCVGKPFKLNFNPVDPDLDSIDIQLDQPLNSLNNAAYNPGVNPL
                     PIPFVTGFDYQNPTPTTGGSTGAQIDPQTGEISFLSLVTGQFNVKFKVTSYRNGLKIS
                     EVVHEMQLIVTNCTGNNTAPTITPPFAGTFQTTVVAGNVVNFNLASTDVELLQDGTPQ
                     TNTILSSGLMFGPNPTVNAGCLVGPCPTVSAAPATGTQGASLTFNWQTDCAHLLDANG
                     NELDVVPYQFVFRAQDNYCPIPEVTYETVTVNVVNPGVIQAPKISCIQGNGAGDFTIN
                     WTPVADPNGTFVEYRIQTVQNGVIATIPAIGTSSYTHTGVTQDLDYFITVVSGCNGQA
                     YRYSDTISNIYLDISNLNPGTAVLDWNNPTSPALPTMGGYYYIYREYPTGTWTLIDSV
                     PYGVTHYEQVIDICGSFLNYQIGLKNTPCNFTSQVNGALFTDQTPPDIPSVVSVTIDT
                     LTNATTINWTMPPQPDVGGYIVYMQDPQTGFLIEVDTIYGKANVSYTYNEPYTQGPVT
                     YTVAAFDTCPSPTGDPFNLSARDPNFHTTIYLKNTLNVCSGKVQLNWTAYGGWPVATY
                     DVYQKTASSGVWQLVASTNNLSYSFTGQNLEKYYFAIKANAANGVFAFSNVDSFQVKR
                     TAQPSFSYIRYATVKPSNDAVEIEYAYDANAIVTKIALQRLKNGVFETIATVDSPSSP
                     HVFVDEDVYVNEESYTYRVLFYDSCGNEGYASNSAKTILLQTQIDNAQVKAYINWTPY
                     QNFNGSILWYYLYRQIDDVYATTPIATLPATTLSYEDDLYDVATEGKVCYRVEAVEGP
                     NVFNDPRSSVSNLSCVLVEPLIFIPNTFTPEGVNPVFIPVLRNYDVDNYKFTVLNRWG
                     QVAFHTTSPYEGWNGLIGNTGKKCEPGTYIYIVEMYNGFGEQVVTRGHVTLLR"
     gene            4487..4780
                     /locus_tag="PKN22_01725"
     CDS             4487..4780
                     /locus_tag="PKN22_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNS41450.1"
                     /translation="MVIISKTTLVEFGKRNTASIDSLNKWYDEAKNANWGNFSEMKKT
                     FNSVDAVGNDRYVFNIKGNNYRIVAMIFFDIRTIFIRFIGTHKEYDKIDCKTI"
     gene            4797..5189
                     /locus_tag="PKN22_01730"
     CDS             4797..5189
                     /locus_tag="PKN22_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS41451.1"
                     /translation="MNSLQIKSKKEYDEVMARIEKLLQKSTELGGFDKLSDLDRKSLA
                     DLSLLAENYEDNIPLMPIKTPETLADMLRYKMYEKDLKQKQMASLLGVSEAFISGLLS
                     GKRKLTIELAKNLHTKLNIDAHFLLMTA"
     gene            6038..8065
                     /locus_tag="PKN22_01735"
     CDS             6038..8065
                     /locus_tag="PKN22_01735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNS41452.1"
                     /translation="MNLLNQPFNTPFDAAPFSKIKNCDFIPAFEAAIATAKNEIQDII
                     TNAATPTFENTVEAMTYSGMQLERISSIFFNLHSAETNDEIEQIAQDITPKLTEYSND
                     IILNPQLFERIKAVYDQRSELTLTPEQQMLLETTYKSFARNGALLDETAKAKLRELDN
                     ELAVLKLQFGKNILDDQNAYRLHITDEKDLAGLPHGAIEAAHEMAKSEGKEGWVFSLD
                     YPSLIPFLTYADNRELRKEISLANGRKAFQNNAYNNEQTVLKIARLRYERAQLLGYES
                     HAHFVLEERMAKTPETVKAFLNDLLVKAKPAAQKEFKELEAFAKELDGIDRLEKWDAS
                     YYAEKLKQKLFSLDDELLKPYFQLENVLNGVFEVAQRLYGLHFQEIQNVDKYHDEVRT
                     YEVKDENDNFVALFYADFFPRKGKRNGAWMTSFKSQYLQNGINERPHVSIVCNFTKPT
                     ATKPSLLTFNEVTTLFHEFGHALHGMLANTIYPSLSGTSVYWDFVELPSQVLENWCYE
                     KETLELFAKHYQTGELIPMEYVQKIKESSNFQEGMATLRQLSFGLLDMGWHGQNPSNI
                     NDLKSFEVEQFAATQLYPDVKDNAMSPSFSHIFNGGYSSGYYSYKWAEVLDADAFEYF
                     KENGIFNRTIADKFKANVLSKGGTEEPMELYERFRGKKPTVDALLKRAGLL"
     gene            8194..9516
                     /locus_tag="PKN22_01740"
     CDS             8194..9516
                     /locus_tag="PKN22_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS41453.1"
                     /translation="MKFYNRENELAELSRIQQLAFTDCSRLTVITGRRRIGKTSLILK
                     ATASTPTVYLFVGRKNEAALCAEFSQIITQSLDVFIPSEIKTFRSLFQTLLEVAKTKS
                     FTLVIDEFQEFYTINSSIYSDIQHIWDLYRKETKMNFIVSGSIYSLMQQLFLNNKEPL
                     FGRADNILKLKPFDLVTLKQIISDYYPTYTNDDLLALYAFTGGVPKYVELLCDNQKLS
                     VNEMIAFMVRDNSLFTEEGRYLLIEEFGKNYGTYFSILAAISSGINTQSEIEAKLGDK
                     NLGGHLKRLIEDYNIIRRVRPILAKEGTLAVRYEIEDHFLKFWFNYFERNRSMVEIKN
                     FGALQNLILDNYPTYSGIMLERYFTQQFIESGNYRAIGSWWELKGNQHEIDIVALKLE
                     KNKAIAVEVKRQRKNFKPEIFAEKVAYLKNKHLNNYDIEQICLTLEDM"
     gene            9593..10072
                     /locus_tag="PKN22_01745"
     CDS             9593..10072
                     /locus_tag="PKN22_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNS41454.1"
                     /translation="MAEASIQLHGTKQEQYEQLLPIIKALVADETDVVANLANVAAAL
                     KQGFGFFWVGFYLVKNDELVLGPFQGPIACTRIKKYRGVCGAAWQQKQTVLVPNVDEF
                     PGHIACSSDSKSEIVVPILRNEEVVAVLDVDSNQLNDFDEVDFKYLNKLCAWLSDLL"
BASE COUNT         3266 a         1814 c         1882 g         3242 t
ORIGIN      
        1 caattaaaaa gatgggaaaa ccaagaataa gtgccaacaa ggataaaatc aattgacgaa
       61 taaacatgcg tgaacgaatc cctctgtcta aaaaatcgtt tttgatatcc atttttttga
      121 tgttccaatt caaattggct aataaatttt ggattttttc aattttttga ggtttttcta
      181 tactgtagag ctcaattcta tcgcggattt ttttcattaa atccaattcc gattcaacat
      241 ttttagcatt gttcaaataa cgcgattgca acgcttcggt tagtgaagtt accaacgcct
      301 cttgttcttt attttgcgtg gtaattagca cttgttccaa tatttctctg aatttttgcg
      361 tcaatttttt tgcagcggtt gaatggttgg ttttgtattc ttccaaataa tcagtcaccg
      421 ttattccctc accaatatta accatgatgg aacttctgaa tttttcacct ttggtataca
      481 tcaatccaat agggatgact tttaaattca attttccacc gttgcgttgt tcggcttcca
      541 aagcaattct tgctgtccca gattttaact tgcgcagcag caattccatg taacttgatc
      601 cttctggaaa cacaaccaaa gttttgcctt ccgaaagaat tttatagcat tcttccaacg
      661 tagaattgtt gttcacccct tcaatttttg cttcatccgc cctgttaatt ggaatcatgt
      721 tgagtgaacg caaaaaccac atttttagct tgttgttaaa aaacgtagcc ttggtcatgt
      781 agtaaattgg ttctttggat atggtgccaa tcaaccaagc atccatcagc gcattgggat
      841 ggttggaaat aatgattttg ggtccgttgt gttgcaagtt cacaccattg cgcactttta
      901 cttccgaata gtaaatgcga atacctatac gtactaaaaa ttttagaatg ttgtatagca
      961 tggaattaac ttacttttta caatgcatgc aaaaatacct aaaaaaatat tcaaaaacag
     1021 ctactttttt gctttttata cgtcaatgaa atactaattt tgtataaaat tcgttaacaa
     1081 ctcagtaata gaaacaaatt gcgcataggt ctacttctta ttatgctgct ttttttattt
     1141 aaagcagaag cttctcatat catgggagga agtattacct atgaatgcga tggtgcgggc
     1201 aattatgttt ttcaattggt tttttaccgc gattgccacg gtgctcatgt aaataccaac
     1261 aatcaaacat tgaaattgtg gaaccatcca acgctttcta ccatttctgt tgcctatgtt
     1321 tctaccgaag atatttcacc tcaaggaacg caagctacgg gtggtcctgc atgctttgat
     1381 tgcaacgacc caaacggcaa tataggcatt ggctccattg aacgcattac ctatcgcagt
     1441 gcacccattt ctatttcggg tgtgccacca gcgcaaggtt gggtagtaac atttgatgat
     1501 ttttcaagaa atggaggcat caccaatttg caaacaccat tgaattatgg cattacgtta
     1561 actgcaaaaa ttttcaatgt taattaccaa aacaatacgt gtaatgacaa ttcggttcaa
     1621 ttcaaacaaa tgcctcattt tgttggttgt gtgggcaaac cttttaaatt gaatttcaat
     1681 ccagttgacc ccgatttaga ttctatagac attcagttag atcaaccact caatagtcta
     1741 aataacgccg cctataaccc aggtgtaaat ccattgccta ttccatttgt taccggtttt
     1801 gattaccaaa atccaactcc aacaacgggc ggaagcacag gcgcacaaat tgacccacaa
     1861 actggagaaa tttctttttt aagcttggtt acaggacagt ttaatgtgaa attcaaagtt
     1921 acttcgtatc gaaatggttt gaaaatatca gaagttgtac atgaaatgca gttgatagtt
     1981 accaattgca cgggcaacaa tactgcacca accatcacac cgccatttgc aggaactttt
     2041 caaaccactg tggtggctgg aaatgttgtt aattttaacc ttgcatcaac cgatgtggaa
     2101 cttttgcaag atggcacacc tcaaaccaat accattcttt cttcgggatt gatgtttgga
     2161 cctaatccaa ctgtcaatgc aggatgtttg gtgggaccat gtcctacggt ttcagcagca
     2221 cccgctacag gtacgcaagg agcatcgttg acctttaact ggcaaactga ttgtgcccat
     2281 ttattggatg ccaatggaaa tgaattggat gtggtgccgt atcaatttgt gtttagagca
     2341 caagataatt attgccccat tccagaagtt acttatgaaa ctgtaacggt gaatgtggtg
     2401 aatccaggtg taattcaagc gcctaagatt tcgtgcattc aaggaaatgg agctggtgat
     2461 tttacaatca attggacgcc agttgccgat cccaacggaa catttgttga ataccgtatt
     2521 caaacagttc aaaatggggt gattgcaaca ataccagcca ttggaacatc gtcatacaca
     2581 catacaggag taactcaaga tttggattat ttcatcacgg ttgtttctgg gtgtaacgga
     2641 caagcctatc gttattctga tactatttcg aatatttatt tggatataag caatttgaat
     2701 cctggaacag cggttttaga ttggaataac cctacatcgc cagcattgcc aactatgggg
     2761 ggctattatt acatttacag agaatatcca actggaacgt ggacgctcat tgattcggtg
     2821 ccttatggtg taacacatta tgaacaagtg attgatattt gtggttcgtt cttgaattac
     2881 caaattgggt taaaaaatac accttgtaac tttacatcgc aagtaaatgg agcattattt
     2941 accgatcaaa caccacccga tattccatct gtggtaagtg ttactattga tactttaacc
     3001 aatgctacta ccattaattg gaccatgccg ccacaacccg atgtgggagg ttatattgtg
     3061 tatatgcaag acccacaaac tggattttta attgaggtag ataccattta tggaaaagca
     3121 aatgtaagtt acacgtataa cgagccgtac acacaaggtc ctgtaaccta tacggttgca
     3181 gcgtttgaca catgtccatc accaacagga gatccgttta acctttcggc acgtgacccc
     3241 aattttcata ctaccatata tttaaaaaat acgcttaatg tttgttctgg aaaagtgcaa
     3301 ttgaactgga cagcttatgg tggttggcct gttgcaacgt atgatgttta ccaaaaaaca
     3361 gcttcgagtg gggtgtggca attagttgct tctaccaaca atttaagtta ttcatttaca
     3421 ggtcagaatt tagaaaaata ttattttgcc attaaagcca atgcagcgaa tggggttttt
     3481 gcattttcca atgtggattc tttccaagta aaacgaacag cacaaccatc gtttagttac
     3541 attcgttatg caaccgttaa accttccaac gatgcagtag aaatagaata tgcctacgat
     3601 gcaaatgcga tagtaaccaa aattgcatta caacgattga aaaatggtgt gtttgaaacc
     3661 attgcaaccg ttgattcacc ttcatcacca catgtttttg ttgacgagga tgtgtatgta
     3721 aatgaagaaa gttatactta tcgtgtgttg ttttacgata gttgtggcaa cgagggatat
     3781 gcttctaaca gtgcaaaaac cattttattg caaacccaaa ttgataatgc gcaggttaaa
     3841 gcctatatca attggacacc ttatcaaaac tttaatggta gtattttatg gtattatttg
     3901 taccgccaaa ttgatgatgt ttacgccact acgccaattg ctactttgcc tgcaacaacg
     3961 ttgagctatg aagacgattt atacgatgtg gcaacagaag gtaaagtttg ctatcgcgtt
     4021 gaagcagttg agggaccaaa tgtattcaac gatccacgtt caagtgtttc caacttatcg
     4081 tgtgttttgg tggaaccgct gatttttatt cccaacacgt ttacaccaga gggcgttaac
     4141 cctgtgttta ttcctgtgtt gcgcaattat gatgtagaca actataaatt caccgtattg
     4201 aaccgttggg ggcaagttgc ttttcacacc accagtccgt atgaaggttg gaatggcttg
     4261 attggcaata ccggcaaaaa atgcgagcct ggaacctaca tttacatcgt tgaaatgtat
     4321 aacggttttg gagaacaagt tgtaactcgc ggtcacgtta cactgctgcg ttagtaataa
     4381 acaccattat tctacattcg caaatttgtt gaggaacaat ttaatctatg ctaattaaat
     4441 ttcatttatt gaaagaaaat tattattttt gtaacataat tttaaaatgg ttattatatc
     4501 gaaaactact ttagtagaat ttggaaaacg aaatactgct tcaattgact ctctgaataa
     4561 atggtatgat gaagcaaaaa atgcaaattg gggaaatttt tcagaaatga aaaaaacctt
     4621 taattcagta gatgcagttg gaaacgacag gtatgttttt aatataaaag gaaataatta
     4681 cagaatagtg gcaatgattt tctttgatat acgcaccatt tttattcgtt ttataggaac
     4741 acacaaggaa tacgataaaa tagattgtaa aacaatttaa gtaaaattat aaagttatga
     4801 actcactaca aataaaatcg aaaaaagagt atgacgaagt aatggcacgt attgaaaaat
     4861 tacttcaaaa atcaacagaa ttgggaggtt ttgacaaact ttcagactta gatagaaaat
     4921 ctttagcgga tttatcattg cttgcagaaa attatgaaga caatattcca ttaatgccga
     4981 ttaaaacacc tgaaacttta gccgacatgc ttagatacaa gatgtatgaa aaagatttaa
     5041 agcaaaaaca aatggcatca ttactcgggg taagtgaagc atttatttca ggattattat
     5101 caggaaaaag aaaattaact attgagttag ccaagaattt acataccaaa ttaaatattg
     5161 atgctcattt tcttctaatg acagcttaat ttatattttc ctctctgcgc cctctgtgcc
     5221 tcctttgcgc ccattgcggt taaaaacgaa taccttgtta attcccttgc gttctttgag
     5281 gttattattg aattatgaat tgtgagttgt ttccctttgc gcccattgcg cctccattgc
     5341 gcccattgcg gttaaaaaat aatacttctg agtaatttat tctcactccc gcatccctcg
     5401 atatgcttcg tttcactgca gaatttttta aataaataaa aagtgatacc aacctccctc
     5461 cttgaggagg gaaagaggga ggtgatagca catacaattc gctttttaca ttgtattatc
     5521 catgattaaa tttaatatct taatgatggt tactttcttt tattcatctc attaaattag
     5581 atagtttaat ctagaaaaac aattgggttt aatatctatg aatcgttttt ttgttgcctg
     5641 taacttactt atttctttac acatcaccac ccttaatccc tttccgcttc tcggaagaga
     5701 tatgttctca ctcaataatt agtttgaaca acaatttaaa atagtatatt aataatgagt
     5761 gtattgttag aatatctcaa taattacaaa agtcacctcc cttaatccct cctcaaggag
     5821 ggaaacatgt ttctactcaa aaaaataatt agaacaccaa tttttgattt ttatatccct
     5881 ttgcgttctt tgcggttaaa aactaatacc ttgttaattc ccttgcgttc tttgctgtta
     5941 aaaatgtatc ctaattcaaa attgatacac cacaaacctc aaactctttc ttttcttata
     6001 tcttttcatt aatttcgctc atctaaaaat caaacagatg aatcttttaa atcaaccgtt
     6061 taatacgcct tttgatgcgg cgcctttttc aaaaattaaa aactgtgatt ttattcccgc
     6121 ttttgaagct gcaattgcaa ctgcaaagaa tgaaatacaa gatattataa ccaacgcagc
     6181 cactccaaca tttgagaata cagtagaagc aatgacgtat tctggaatgc aattggagcg
     6241 catatcatcc attttcttta atctacattc tgctgaaacc aatgatgaaa tagaacaaat
     6301 tgctcaagat attacaccta aattgacaga atatagcaac gatattatcc tcaatccgca
     6361 actatttgag cgtataaaag cggtttatga tcaacgaagt gagttgacat taacgcccga
     6421 acaacaaatg ttgctcgaaa caacctataa atcttttgct cgaaatggtg ctttgttaga
     6481 cgaaacagca aaagcaaaat tgcgtgaatt ggacaatgaa ttggcggtct tgaaattgca
     6541 atttggtaaa aacattttag acgatcaaaa tgcgtatcgt ttgcacatta ccgacgaaaa
     6601 agatttggct ggattgcctc atggagctat tgaagcagca cacgaaatgg caaagtcaga
     6661 aggaaaagag gggtgggttt tttcgttgga ttaccccagt ttaattccct ttttgacgta
     6721 tgcagacaat cgtgaattac gtaaagaaat ttcattggca aacggacgta aagcatttca
     6781 aaacaatgcg tataacaatg agcaaacagt cttgaaaatt gcacgtttac gctatgaacg
     6841 cgctcaattg ttgggctatg aatcacacgc tcattttgtg ttggaagaac gaatggctaa
     6901 aactccagaa accgtaaaag catttttgaa tgatttgttg gtaaaggcaa aacctgctgc
     6961 ccaaaaagaa tttaaagagt tagaagcttt tgcaaaagaa ttggatggca tcgatcgttt
     7021 agaaaaatgg gacgcaagct attatgccga aaaactcaaa caaaaattgt tttcacttga
     7081 cgatgaattg ttaaaaccct attttcaatt agaaaacgtg ctcaatggtg tttttgaagt
     7141 tgcacaacgt ttgtatggat tgcattttca agaaatacaa aatgttgata aataccatga
     7201 tgaggttaga acttacgaag taaaagacga aaacgataat tttgtagcac tattttatgc
     7261 cgatttcttt ccacgtaaag gaaaacgcaa cggtgcttgg atgacttcgt tcaaatcgca
     7321 atatttgcaa aatggcatca atgaacgccc acatgtttcc attgtgtgta atttcaccaa
     7381 accaactgca accaaaccat ccttgttgac ttttaacgaa gttacaacct tgtttcatga
     7441 gtttggacat gccttgcatg ggatgttggc aaatacaatc tacccaagtt tatcaggaac
     7501 ttcggtgtat tgggattttg tggaattgcc atcacaagta ttggaaaatt ggtgttacga
     7561 gaaagaaacc ctggagttat ttgccaaaca ttaccaaaca ggtgagctaa ttccaatgga
     7621 atatgtacaa aaaatcaaag aaagctcaaa ttttcaggaa ggaatggcaa cattgcgcca
     7681 attatcgttt ggtttattgg acatgggatg gcacggacaa aatccatcca atattaacga
     7741 tttaaaatca tttgaagtgg aacaatttgc tgccactcaa ctttatcctg acgtgaaaga
     7801 caacgccatg agtccttcat tttctcacat ttttaacggt ggctattcgt cgggttatta
     7861 ttcctacaaa tgggccgaag tattggatgc cgatgcattt gaatatttta aagaaaatgg
     7921 aatttttaat cgcactattg ctgataaatt caaagcaaac gtattgtcga aaggcggaac
     7981 cgaagaacca atggaattgt atgaacgttt tagaggtaaa aaaccaaccg ttgacgcttt
     8041 gttgaaacgt gcaggattgc tataaacttt aaaaatcatt gataaacaga tagttgttct
     8101 ggtaacggcg gtaacagtta cggcggttac cagaatttgt tttattgaat atttccattt
     8161 atctttgtaa aactcaagca ataaaattgt agtatgaagt tttataaccg agaaaatgaa
     8221 ttagctgaat taagtagaat ccaacagttg gcttttacag attgctcgcg tttaactgta
     8281 attacaggta gacgaagaat tggcaaaact agcttgattt tgaaagcaac ggcttcaaca
     8341 cctactgttt atttgtttgt tggacgcaaa aatgaagcgg ctctctgcgc agaattttcg
     8401 caaataatta cacaatcatt ggatgttttt attccttctg aaattaaaac atttcgttct
     8461 ttatttcaaa cgttgcttga agttgctaaa acaaaatcgt ttactttagt gatagatgaa
     8521 tttcaagagt tttacacgat taattcttct atttatagcg atatacagca tatttgggac
     8581 ctgtatcgaa aggaaacaaa aatgaatttt attgtttctg gctctattta ttcgttgatg
     8641 cagcaactgt ttttaaacaa caaagaaccg ctttttggga gagcagataa tatactgaaa
     8701 ttaaaacctt ttgatttagt cacgttgaaa caaataatta gtgattatta tccaacatac
     8761 accaacgatg atttattggc attgtatgct tttactggtg gagttcctaa atatgtggag
     8821 ttattgtgcg ataatcaaaa attatctgtt aacgagatga ttgcttttat ggtacgcgac
     8881 aattccttgt ttactgaaga aggcagatat ttgttgattg aagagtttgg aaaaaactat
     8941 ggaacctatt tttccatcct agcggcaata tcaagcggta ttaatacgca gtcagaaata
     9001 gaagcaaaat tgggagataa aaatttaggc ggacatttaa agcgtttaat tgaagattac
     9061 aatattattc gaagagttcg tcctattctt gcaaaagaag gtacgttggc agtacgatat
     9121 gaaattgaag atcactttct taaattttgg tttaattatt ttgaacgcaa tcggtcaatg
     9181 gttgaaatta agaactttgg tgcattgcaa aatctgattt tagacaatta tcccacctat
     9241 tctggaataa tgttggagcg ttattttacc caacaattca ttgaaagtgg taactatagg
     9301 gctataggtt catggtggga attgaaaggc aaccaacatg aaatagatat tgttgcgctt
     9361 aaacttgaaa aaaataaagc aattgcagtt gaggtaaaac gacagcgaaa aaactttaaa
     9421 cccgaaattt ttgcagaaaa agtagcttac ttaaaaaaca aacatcttaa taattatgac
     9481 attgaacaaa tttgtttaac gttggaagac atgtaatttt ctctcaccat atttccactt
     9541 ttaagttttt gacaaatcaa attattttaa ctttgttgca ttaatcattc aaatggcaga
     9601 agcatctatt caattacacg gaacaaagca agagcaatac gagcaattgt tgcccattat
     9661 caaggcactt gttgcggacg aaacggatgt tgttgccaat ttggctaacg ttgctgcggc
     9721 actcaagcaa ggttttggtt ttttctgggt aggcttttat ttggttaaaa atgatgagtt
     9781 ggtgttaggg ccatttcaag gaccaattgc ttgcacacgc atcaaaaaat acagaggtgt
     9841 ttgtggcgcg gcttggcaac aaaagcaaac tgttttagtg cccaatgtag atgaatttcc
     9901 aggccatatt gcatgtagtt ctgactccaa aagtgagatt gtggtaccca ttcttcgcaa
     9961 tgaggaagta gttgctgtgc tggatgttga cagcaatcaa ttgaatgatt ttgatgaggt
    10021 ggattttaaa tatttaaaca aattatgcgc ttggctttca gatttattgt aattgcactg
    10081 ttattggctg cttgtgcgca agtaaaacct ttggaaggcg gtccgcgcga tgaagcagca
    10141 cctgttccat ttggagttta tccacctaat ggaagcacca atttttctgg tcaagcaatt
    10201 gcca
//
LOCUS       DAOQVQ010000029        10193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7381_length_10193_cov_3.731308, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000029 DAOQVQ010000000
VERSION     DAOQVQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7381_length_10193_cov_3.731308"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKN22_01750"
     CDS             complement(<1..466)
                     /locus_tag="PKN22_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNS41455.1"
                     /translation="MFKKILIANRGEIALRVIRTCKEMGIKTVAVYSTADKESLPVRF
                     ADEAVCIGPALSSKSYLSIPNIIAAAEITNADAIHPGYGFLSENEKFSRICQESGIKF
                     IGATPEQIAGMGDKASAKATMIAAGVPCIPGSDGLLKDIEDAIFIREVSNDFI"
     gene            complement(538..1038)
                     /gene="accB"
                     /locus_tag="PKN22_01755"
     CDS             complement(538..1038)
                     /gene="accB"
                     /locus_tag="PKN22_01755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNS41456.1"
                     /translation="MNIREIQDLIKFVAKSGVTEVEIEQKDFKITIKACGTEPVERQI
                     VVQAAAPVHQLAPAPVAVPAAAPAPVATPAAPATPAAAPAENSNLITIKSPMIGTFYR
                     APGVDKDPFVSVGQTINTGDTVCIIEAMKLFNEIESEISGKIVKVLVDDATPVEYDQP
                     LFLVEP"
     gene            complement(1075..2064)
                     /locus_tag="PKN22_01760"
     CDS             complement(1075..2064)
                     /locus_tag="PKN22_01760"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNS41457.1"
                     /translation="MEKISAAITGVFGYVPDEVVTNEDLAKIVDTSDEWITTRTGIKE
                     RRILREGGSSDIAAAAVKGLLEKKGIDPLEIDLLIVATVTPDYPFPSTSNVVCDKLGM
                     KNAWGYDLIAACSGFIYGLSTGAQFIETGRYKKVVVVGVDKMSAILDYQDRTTCVIFG
                     DGGGAVLLEPNNEGFGVKDFILHADGAGRQYLLQPAGGSLHPASVETVEKRMHYVKQE
                     GKTVFKFAVTNMAEVSAQIMEKNNLSSEDVDWLVPHQANLRIIDATAERMGLPKEKVM
                     INIQKYGNTTAGTLPLCLWDYEKQLKKGDNVILSAFGGGFTWGAVYVKWAYNS"
     gene            complement(2066..3007)
                     /gene="plsX"
                     /locus_tag="PKN22_01765"
     CDS             complement(2066..3007)
                     /gene="plsX"
                     /locus_tag="PKN22_01765"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNS41458.1"
                     /translation="MKIGIDILGGDFAPDANISGAVLARKELPTDARIVLIGDKDQIF
                     SGLSSLGENPNDYEVVHAPDVITMHDHPTKAIPSKPNSSIAVGFDLLAQKEIDVFAST
                     GNTGAMLVGSIFKLGTINNILRPCITTVMPTIDGGQSILLDVGANSDCKPEVLQQFAV
                     LGSLYSKHVFGNEQPKVALLSIGEEESKGNIATVAAHKLIAESNELNFIGNIEGRDLF
                     VGKADVVVCDGFTGNIVLKQAEGMYGLLRKRGIKDDAYFNRFNYENYGGTPVLGVNGN
                     VIIGHGISNDIAVKNMILLAHETAKSQLPSKINEAFN"
     gene            complement(3031..3222)
                     /gene="rpmF"
                     /locus_tag="PKN22_01770"
     CDS             complement(3031..3222)
                     /gene="rpmF"
                     /locus_tag="PKN22_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNS41459.1"
                     /translation="MAHPKRRVSTTRRDKRRTHFKAVANNVATCPTTGQPHLFHHAYW
                     HEGKLYYKGKVVAEKEVAI"
     gene            complement(3246..3854)
                     /locus_tag="PKN22_01775"
     CDS             complement(3246..3854)
                     /locus_tag="PKN22_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNS41460.1"
                     /translation="MAGSKREFVIPFVGLKIGSHTFEFDISDSFFEDIEYSIIHKANV
                     HVDLVLEKKETMMVGNFSCEGEVSTLCDRCNDPLSVPVKGSFQLIFKQSTQTSDDESL
                     IILAPEAYELNVSPYIYELITVALPSRMVHKEGECNEEMVAALSQYLVNNYEEDEEDW
                     DEYDEDEEDFDDDDFEDEDFDDDDDTPDDDIDPRWSILKNLN"
     gene            4050..4418
                     /locus_tag="PKN22_01780"
     CDS             4050..4418
                     /locus_tag="PKN22_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNS41461.1"
                     /translation="MKAFLTILLLVASNTFMTFAWYGHLKLKEYSWFSNFSLFKIILF
                     SWAIAFFEYSIMVPANRIGFEGTGGPFSLWQLKVIQEVITLVVFTVFTLVLFKEETFK
                     TNHLIGFVFLILAVYFIFKK"
     gene            4461..5588
                     /gene="hemW"
                     /locus_tag="PKN22_01785"
     CDS             4461..5588
                     /gene="hemW"
                     /locus_tag="PKN22_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNS41462.1"
                     /translation="MAGIYIHIPFCKQKCTYCDFHFSTTYHAYKKRMMDAICKEIERQ
                     KDYLNNQSIHTIYFGGGTPSLLTHNELHQILSTIHAHFDTSLVEEITLEANPDDVTIT
                     AVEQWKSLGINRLSIGLQSFKASDLQWMNRAHSVDEAKKCVEIAINSGIPNITVDLIY
                     GLPNLSNEEWKNHIEMAVNMGVQHVSAYCLTIEQGTGLSHQVAKGKITPATEQNQVEQ
                     FLILVETLEKYGFEQYEISNFCKPGFESKHNSSYWKGIEYLGIGPSAHSFNGTSRAWN
                     VRNNHTYMNAIENNEIWWEEENLSRNDQFNELLLIGLRTSAGVNLEQLNQLLPLTNEF
                     QQTIRSFEKEGLLTNNNNRIQLTKIGRLQADRIASDLFNVE"
     gene            5659..6663
                     /locus_tag="PKN22_01790"
     CDS             5659..6663
                     /locus_tag="PKN22_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNS41463.1"
                     /translation="MRTLLISTLLVLASFTSWGNQKIYSKLNKLYLTDRNKCMEVSKK
                     LIAKKPTTSIPYYFTSVIYYDKSKESQTLKGVYLQLYRSVTNAAKFEKLSSESERSLV
                     NWDEHLVSLKNRAVRLASSLKKNEMDDLSTNLLAGLNKIESVAAENEVVKDDVLNDAD
                     LAQFESTTKKTESVINEASVPTHLFFGMPSGNENVRSSNITEEQNLLALINAERAKNG
                     AQPLTWSEDLARACRYHAYDLGSQGYFSHDSYDVSNGKKTMVGTTFNRIEKFSKRAVD
                     GECIAAGGNTAEKTFKQWMESPGHRKIIMDAGSKTVGIGYQAVENSPFVDYWVLATGK
                     "
     gene            6768..7004
                     /locus_tag="PKN22_01795"
     CDS             6768..7004
                     /locus_tag="PKN22_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HNS41464.1"
                     /translation="MKTIVCILCMALSTLSVLAAHEDVVPSTIKKVTIFTEGAQVYRN
                     AYYSVGKGITKIIIENVSDFVDEKSIQVRQQVTL"
     gene            7001..8443
                     /locus_tag="PKN22_01800"
     CDS             7001..8443
                     /locus_tag="PKN22_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNS41465.1"
                     /translation="MILDTRFSIYQPQPEEFKLEGLPLKIVKEIEQLSDSIETVNFSI
                     QTIQNEIDVLNATKNILINNGAVNGKGKVNDSIGLLKATVDYYLLKMNELNASIGKLN
                     LKKEEKMRLKKKLNERLSSLKSYQNSNNLTPKKMGPIPRIVITVQANVDVKGKIDLSY
                     IVSNAGWTPSYDIMSDIENGKVLLNYKAQVYQNTGEKWENVLLSISTNNPYKNKTKPE
                     LHPWYISYYNNYYQQPATPQPVSIVKTESLQKTSSKAEVATNYDSDEAALSASDFTQV
                     INRTISAEFAINLPYTIESNNEPHIVLISKNELNASFLYYTVPKLDNHAYLVARLTKL
                     EELQLVPASASIFFEGSYMGETYIDPSSMSDTLNLSLGKDPNIIVKRTNLKNDVNKKI
                     VGNQIEQTFAYQIEVKNLKSKNVEIIVEDQIPVTQIGEIEIALLDNSKAKYDAVTGKL
                     EWNLKLRPNQMDVLKYKCQIKYNKEKQLAL"
     gene            complement(8481..9902)
                     /locus_tag="PKN22_01805"
     CDS             complement(8481..9902)
                     /locus_tag="PKN22_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNS41466.1"
                     /translation="MKDYSKNLSHPIFKVIRQIAKEENLEVYVIGGFVRDTFLERSRK
                     DIDIVVVGSGMELAQKAAEKLRVKKVAYFKNFGTASFKYKDIDVEFVGARKESYQRDS
                     RKPIVENGTLQDDQNRRDFTINAMGFSLQDHNFGELIDPFNGIGDLEKGIIRTPLDPD
                     ITFSDDPLRMMRAIRFASQLNFKIEASCLEAILRNSERLEIISNERIMDELNKIILSD
                     EPSRGFKLLFSTKLLHRFFPEMVALHGVDTIDGKSHKDNFYHTLEVLDNVAQNSDDLW
                     LRWAAIMHDIAKPPTKRFDPKVGWTFHGHEEIGARMTPKVFARLKLPLDAKMKYVQKL
                     VRLHLRPISLVKGDVTDSAVRRLLFEAGDDIDDLMTLCNADITSKNEFKVKRYRQNFE
                     LVREKLKAVEEKDKVRNFQPPVGGEEIIKTFNLEPCQAIGDIKSKIKEAILEGEIPNE
                     YEAAFALMLKLGKEMGLNPVKSR"
     gene            complement(9918..>10193)
                     /locus_tag="PKN22_01810"
     CDS             complement(9918..>10193)
                     /locus_tag="PKN22_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41467.1"
                     /translation="IFVNKEGKFFVEGSGQLGFLFKELAEGDWREESIRHIIQVSFAY
                     AISADLFVPPYEMMQELNVDQVQTIGHNDQSAIGKRLGFKYRNIQED"
BASE COUNT         3264 a         1964 c         1734 g         3231 t
ORIGIN      
        1 ctataaagtc gttacttact tctctgataa atattgcatc ttcaatgtct ttcaacaaac
       61 catcagatcc aggaatacaa ggtacacctg ctgcaatcat tgtagcctta gcacttgctt
      121 tatctcccat tcctgcaatt tgttcgggtg ttgcaccaat aaacttaatg ccactttctt
      181 gacaaatgcg cgaaaacttt tcattttcag aaagaaaacc atatcccggg tgaatagcat
      241 ctgcatttgt aatttcagct gctgcaatga tgtttggaat acttaaatag gatttactgc
      301 tcaaagccgg accaatgcat actgcttcgt ctgcaaaacg tacaggcaaa ctttctttat
      361 cagcagttga ataaacggct actgttttga ttcccatttc tttacacgtt ctaataacac
      421 gtaaagcaat ctctcctcta ttggcaatta atattttttt aaacataatt ctaactggtt
      481 agctaacaaa aaaaaaggag cgctagagtc tcctttataa ttctttattt tttttgatta
      541 aggttcaact aagaacaatg gttgatcgta ttcaactgga gtagcatcat ctaccaaaac
      601 tttaacgatt ttaccgctaa tttcagattc aatctcgttg aacaacttca ttgcttcaat
      661 aatacaaact gtatctcctg tattgattgt ttgtcccaca gatacaaatg gatctttatc
      721 tactccagga gcacgataga atgtcccaat cattggtgat ttaatagtaa ttaaatttga
      781 attttctgct ggagctgccg caggtgttgc tggagctgca ggtgttgcaa ctggtgctgg
      841 tgctgccgct ggaacagcaa ctggtgctgg agctaattga tgaactggag ctgctgcttg
      901 cacaacaatt tgacgttcaa ccggttcagt accacatgct ttaattgtta ttttgaaatc
      961 tttttgttca atttctactt ctgttacacc tgattttgca acaaatttta ttaaatcttg
     1021 tatctctcta atattcattt tgaataaatt ttatcttgta aatatactat ttttttatga
     1081 attatatgcc catttaacat aaactgcacc ccatgtaaat ccacctccaa aagcagataa
     1141 aatgacatta tctccttttt tcaattgctt ttcatagtcc cacaagcaca aaggcaaggt
     1201 tcccgcagtt gtatttccat acttttgaat gttgatcatc accttttctt ttggcaatcc
     1261 catacgttct gcagttgcat caataatacg caaatttgct tggtgaggca ctaaccagtc
     1321 tacgtcttca cttgacaagt tgtttttctc catgatttgt gcagaaactt ctgccatgtt
     1381 ggttaccgca aacttgaaaa ctgtcttccc ttcttgcttc acgtaatgca tacgtttttc
     1441 aaccgtttca acagacgctg gatgtaatga acctcctgct ggttgcaata aatattgacg
     1501 gcctgctcca tcagcatgta aaataaaatc ttttacacca aaaccttcgt tatttggttc
     1561 caataaaaca gctccaccac catcgccaaa aatcacacat gttgtgcggt cttgataatc
     1621 taaaatagcc gacattttat ctacaccaac tacaacaact tttttgtagc gtccagtttc
     1681 aataaattga gcacccgttg ataaaccata gataaaacca gaacaagcag ctataaggtc
     1741 gtagccccaa gcatttttca ttcctaattt atcacaaacc acgtttgatg tgcttggaaa
     1801 aggataatct ggtgttactg ttgcaacaat caataaatcg atttccaaag gatcaattcc
     1861 tttcttttct aaaagacctt ttacagctgc tgcggcaata tcagaactac ctccttcgcg
     1921 taagattctt ctttccttaa tccctgttct tgttgttatc cattcatccg aagtatcaac
     1981 gatttttgct aaatcttcgt tggttacaac ttcatctgga acatatccaa aaactcctgt
     2041 tattgctgct gatatctttt ccattttaat taaatgcttc gttaattttt gaaggcaatt
     2101 gtgacttcgc tgtttcatgt gccagcaaaa tcatattttt cactgcgata tcgttggata
     2161 ttccgtgacc aataataaca ttgccgttga cacctaaaac aggcgtacct ccataatttt
     2221 cataattgaa acggttgaaa taagcatcgt ctttaattcc tctttttctg aggaggccat
     2281 acattccttc tgcttgtttc aatacaatgt ttccagtaaa accatcgcac acaacaacat
     2341 ctgctttacc aacaaataaa tcgcgtcctt caatgttccc aataaagttc aattcgttgg
     2401 attctgctat taatttatgt gctgcaacag ttgcaatgtt tcctttcgat tcttcttctc
     2461 caatgctcaa caatgccact ttgggttgct cgtttccaaa aacgtgtttt gaatacaagg
     2521 atcctaaaac tgcaaattgt tgcaacactt ctggtttaca atccgaattg gcaccaacat
     2581 ctaataaaat ggattgacct ccatctatag ttggcatcac cgttgtaata catggacgca
     2641 aaatattgtt aatagttcct aatttaaaaa tagaaccaac caacattgca ccagtgttac
     2701 ccgtacttgc gaaaacatca atttcttttt gggctaacaa atcaaaaccg acggcaatgc
     2761 tgctattggg ctttgatgga atagcctttg ttgggtggtc gtgcatggta atcacgtctg
     2821 gcgcatgcac cacttcgtaa tcatttggat tttcacccaa cgaagaaagg ccactaaaga
     2881 tttgatcttt gtcgcctatt aacacaattc gagcatcagt tggcaattct tttcttgcca
     2941 aaactgcacc agaaatgttt gcatctggag caaaatcgcc tcccaagatg tctataccaa
     3001 tcttcataaa gaagctttac agcttacaaa ttaaatcgct acttctttct cagcaacaac
     3061 tttccctttg taatacaatt ttccttcatg ccagtatgca tggtggaaca aatgtggctg
     3121 acctgttgta ggacaagttg ctacattgtt tgctacagcc ttaaaatgtg ttcttctttt
     3181 gtctcttctt gtagtagaga ctctgcgttt tggatgtgcc attttacttt ataaatatta
     3241 agtttttaat tcaaattttt taaaattgac caacgtggat caatatcatc atcgggagta
     3301 tcatcgtcgt cgtcgaaatc ttcatcttca aaatcgtcgt catcaaaatc ctcctcatct
     3361 tcatcgtatt cgtcccaatc ttcctcgtct tcttcgtaat tatttacgag gtattgacta
     3421 agagccgcta ccatttcctc attacattca ccctctttgt gcaccattcg tgatggcaaa
     3481 gcaaccgtaa taagttcgta aatatacgga gaaacattca attcataagc ttcgggagct
     3541 aaaattatta acgattcatc gtctgatgtt tgtgtgctct gcttaaaaat caattgaaac
     3601 gaacctttaa caggaacact caacggatca ttacatcgat cacacaatgt agaaacttct
     3661 ccttcacaag aaaaatttcc taccatcatt gtttcttttt tttcgagtac caagtccaca
     3721 tgcacattgg ctttgtggat aattgaatac tcgatatctt caaagaacga atctgaaatg
     3781 tcaaactcaa aagtgtgact tcctatcttt aaacctacga aaggtatcac aaattcacgc
     3841 ttacttcccg ccactttatt caaaattgga cggcaaagtt aagcaaatat aaattagaac
     3901 aaagtttttt attggaattt ttatttgttt ttgttgcttt ttaacatcat ttcaactcaa
     3961 tttctattga atttggtctg ttttcactca aaattaattt actatcacct tgaattttgt
     4021 aatattgaac tcaaaaaaat aaaattctga tgaaagcatt tcttaccatt ctccttcttg
     4081 ttgcttccaa tacgtttatg acgtttgctt ggtatggaca tttaaaattg aaagaatata
     4141 gttggttttc taattttagt ttattcaaaa taattttgtt tagttgggcc attgcgtttt
     4201 ttgagtattc catcatggta ccagccaacc gaataggatt tgaaggaaca ggcggaccgt
     4261 tttctctttg gcaattgaaa gttattcaag aagtgattac gttggttgtg tttacagttt
     4321 ttacgcttgt tttattcaaa gaagaaacgt ttaaaaccaa ccatttgata ggctttgtgt
     4381 ttttgatatt ggctgtctat tttattttta agaagtaaat ggcaacttat cgctaatttt
     4441 gtagcaaatc ctcaataaaa atggctggaa tatacattca cattcctttt tgtaagcaaa
     4501 aatgcacgta ttgtgacttt cattttagca ccacctatca tgcgtataaa aaacgaatga
     4561 tggatgccat ttgcaaggaa attgaacgtc aaaaagatta tttaaacaac caatccatcc
     4621 acaccattta ttttggcgga ggaacaccta gtttattaac tcacaacgaa ttacatcaaa
     4681 ttctttcaac aatccacgct cattttgata cttcgttggt cgaagaaata acgttggaag
     4741 ccaatccgga tgatgtgact ataacggctg ttgaacagtg gaaatcatta ggaattaata
     4801 gattaagtat tggtttacaa tcgtttaaag caagtgattt gcaatggatg aaccgtgcgc
     4861 attcggtaga tgaagcaaaa aaatgtgttg aaatagcaat caatagtgga attccaaaca
     4921 ttacggtgga tttaatttat ggcttaccca atttatccaa cgaagaatgg aagaaccaca
     4981 tcgaaatggc ggtcaatatg ggcgtgcaac acgtttcggc ttattgttta accattgaac
     5041 aaggaactgg gttgagtcat caagttgcaa aaggaaaaat aacgccagca accgaacaaa
     5101 atcaagtgga acagttttta attttagttg aaacgcttga aaaatatggt tttgagcagt
     5161 atgaaatttc caatttttgt aaacccggat ttgaatccaa acacaattca agttattgga
     5221 aaggaattga atatttaggt attggtccgt cggcacactc gttcaatgga acctcacgtg
     5281 cgtggaatgt gcgcaacaat cacacgtata tgaacgccat agaaaacaac gaaatttggt
     5341 gggaggaaga aaatttaagt agaaacgacc aatttaatga attattattg attggtttga
     5401 gaacatcggc aggtgtgaat ctagaacaat tgaaccagct tttgccactc accaacgaat
     5461 ttcaacaaac tattcgttcg tttgaaaaag aaggattgct aacaaataat aacaatcgca
     5521 ttcaacttac taaaatcggg cgtttgcaag ccgatagaat tgcttcggat ttatttaatg
     5581 tggaataatt gcaatattta attttttgaa acgttgttaa ttgaagaacc agttagtaac
     5641 tttttaaaat ctaaaattat gcgtactctc ctcatttcta cgcttttggt tttagcttca
     5701 ttcaccagtt ggggcaacca aaaaatctat tcaaaattga ataaacttta tttaaccgac
     5761 agaaacaaat gtatggaagt ttctaaaaag ttgattgcaa aaaaaccaac aacttcaatt
     5821 ccctattatt ttacgtcggt gatttattac gacaaaagca aagaatcaca aacgctcaaa
     5881 ggggtgtatt tgcagttgta tcgctcggta accaatgctg ctaaatttga aaagttgagc
     5941 agtgaaagcg aacgttcttt ggtgaattgg gacgaacatc tagtttcgct taaaaacaga
     6001 gcggttcggt tggctagttc cttaaaaaag aacgaaatgg atgatttgag tactaattta
     6061 ttggctggac tcaacaaaat tgaatctgtt gctgcagaaa atgaagttgt aaaagacgat
     6121 gttttaaacg atgctgattt agcacaattt gaaagtacga ctaaaaaaac agaatcagtc
     6181 atcaacgaag ccagtgtacc tacgcattta ttttttggta tgccatcggg taatgagaat
     6241 gtgcgttcgt ctaacataac tgaggaacaa aatttgctgg cactcatcaa tgctgaacga
     6301 gcaaaaaatg gtgcgcaacc gttgacttgg agtgaagact tagcgcgcgc atgtaggtat
     6361 catgcgtatg acttaggaag tcaaggttat tttagccacg attcttatga tgtttccaac
     6421 ggtaaaaaaa cgatggtggg cacaactttt aaccgtattg aaaaattcag caaacgtgca
     6481 gttgatggtg aatgcattgc tgcgggagga aacacggctg aaaaaacatt taaacaatgg
     6541 atggaaagtc ctggacaccg caaaattatt atggacgccg gttcaaaaac agttggaatt
     6601 ggctatcagg cggtagaaaa tagtccattt gttgattatt gggtgttggc aacgggaaaa
     6661 taataaaata atcttttaat tgttagtgga aagctgtttc gagaaggaac agcttttttt
     6721 atgaacgttt ttggtggtga tttgtacgga taagaaaata aagcatcatg aaaacaattg
     6781 tatgtatcct atgtatggca ttgagcacct tatctgtgtt ggctgcccat gaagatgttg
     6841 ttccatcaac aattaaaaaa gtaaccattt ttacagaagg cgcgcaagtt tatcgaaacg
     6901 cctattattc ggttggaaaa gggattacaa aaatcattat tgagaatgtg agtgattttg
     6961 tggacgaaaa aagcattcag gtaaggcaac aggtgacatt gtgattttag acactcgttt
     7021 ttctatttac cagccacaac ccgaagaatt taagttggaa ggattgccac taaaaattgt
     7081 aaaagaaatt gaacaattat ccgactctat tgaaacggtc aatttttcca ttcaaaccat
     7141 tcaaaatgaa attgatgtat tgaatgccac taaaaacatt ttaatcaaca atggtgctgt
     7201 aaatggcaaa ggtaaagtca atgattctat tggactattg aaagcaacgg ttgattatta
     7261 ccttttaaaa atgaacgaac tcaatgctag tatcgggaaa ttgaacttaa agaaagaaga
     7321 aaaaatgcgc ttaaagaaga aattaaacga gcgcttatct tcattaaaat catatcaaaa
     7381 cagcaataat ttaacgccaa aaaaaatggg accaattcca cgaattgtta tcacagttca
     7441 agccaatgta gatgtaaaag ggaaaattga tttatcctac attgtttcaa atgctggatg
     7501 gacaccatcg tatgacatta tgagtgatat tgaaaatgga aaagtactac tcaactacaa
     7561 agcgcaagtg tatcaaaaca cgggtgaaaa atgggagaat gttttacttt ctatttcaac
     7621 caataatccc tataaaaata aaacaaaacc cgagttgcat ccgtggtata ttagttatta
     7681 caacaactat tatcagcaac ctgcaactcc acaacctgta tcaattgtta aaacagaaag
     7741 tcttcaaaaa acaagctcca aagcagaagt tgccaccaat tatgacagtg atgaagcagc
     7801 actatcagca agtgatttta cgcaagtaat caatcgaaca atttcggcag aatttgccat
     7861 taatttacct tacactattg aatccaacaa tgaacctcac attgttttga ttagtaaaaa
     7921 tgaattgaat gcttcatttt tgtattatac agttccaaaa ttggacaatc acgcctattt
     7981 agtggcgcga ttaacaaaat tggaagaact gcaattggtt ccagcaagtg cttctatctt
     8041 ttttgaaggc agttacatgg gcgagacata catcgatcct agctccatgt cggatacctt
     8101 gaatttaagt ttaggcaaag atccaaacat cattgtaaaa cgcaccaatt tgaaaaacga
     8161 tgtcaacaaa aaaattgtag gtaatcaaat agaacaaacg tttgcttacc aaatcgaggt
     8221 aaaaaactta aaatctaaaa acgttgaaat aattgttgaa gaccaaattc cggttactca
     8281 aataggcgaa attgaaattg cattgctaga caactcaaaa gcaaaatacg atgccgttac
     8341 tggtaaattg gaatggaatt taaaattgcg tccgaatcaa atggatgtgt taaaatacaa
     8401 gtgtcaaatc aaatacaaca aagaaaaaca gcttgcattg taagcaaata caactaacaa
     8461 agccgttagg aagcaaattc ttagcggctt ttcactggat ttaagcccat ttctttaccc
     8521 aatttgagca ttaacgcaaa agctgcttcg tattcattgg ggatttcgcc ttccaaaatt
     8581 gcttctttga ttttactttt gatatcgcca atggcttggc aaggttccaa attaaacgtt
     8641 ttgataattt cctcccctcc tactggcggt tgaaaatttc tgaccttgtc tttttcttca
     8701 acggctttca atttttcgcg taccaactca aaattctgac gataacgttt tactttgaat
     8761 tcattttttg aggtaatatc agcattgcat aaagtcatta aatcgtcaat atcatcacct
     8821 gcttcaaaca acaatcggcg aactgctgag tcggtaacat ccccttttac caatgaaatc
     8881 ggtcgcaaat gcaaacgcac caatttttga acgtatttca ttttggcatc caaaggcaat
     8941 tttaaacgag caaagacttt aggcgtcatg cgcgcaccaa tttcttcatg tccgtgaaag
     9001 gtccaaccaa ctttaggatc aaatcgtttt gtgggtggtt ttgcaatatc gtgcataatt
     9061 gctgcccagc gcaaccataa atcatcgctg ttttgtgcaa cattatccaa tacttccaag
     9121 gtatggtaaa aattgtcttt atgcgattta ccatcgatgg tatccacgcc atgaagcgcc
     9181 accatttctg gaaaaaaacg atgcaacaat ttggttgaga acaacaattt aaaaccgcgt
     9241 gaaggctcat cggataaaat aattttattc aattcatcca tgatacgctc gttggaaata
     9301 atctccaaac gttccgaatt tcttaaaatt gcttccaagc aactggcttc tattttgaaa
     9361 ttaagttgcg aagcaaatcg aatggcgcgc atcattctaa gcggatcatc gctaaaggta
     9421 atatcagggt ctaaaggtgt tcggatgatt cctttttcca aatcgccgat tccattaaaa
     9481 ggatctatca actcaccaaa attgtggtct tgcaacgaaa aacccatagc attaatggta
     9541 aaatcgcgtc ggttttgatc gtcttgcaac gtgccgtttt ctacaatagg tttgcgagaa
     9601 tcacgttgat atgattcttt tctagcacct acaaattcca catcaatgtc tttgtattta
     9661 aaagaagctg ttccaaaatt cttaaaatag gcaacttttt ttacacgcaa tttttcggct
     9721 gctttttgtg ccagttccat tccgctacct acaacaacta tatcgatgtc ttttctgctt
     9781 ctctccaaaa aagtatcgcg cacaaagcca ccaatcacat agacttccaa gttttcttct
     9841 ttggcaatct gacgaatgac cttaaaaata gggtgcgata aatttttaga ataatctttc
     9901 attatcaatt aagaagatta atcttcttga atatttctgt atttaaaacc aagacgttta
     9961 ccaattgccg attgatcgtt atggccaatg gtttgaactt ggtcaacatt taattcttgc
    10021 atcatttcat aaggcggaac aaataaatct gctgaaatgg cataagcaaa cgacacttga
    10081 ataatgtgac gaatggattc ttctctccaa tcgccttctg ccaattcttt gaacaaaaat
    10141 ccaagttgac cgcttccttc cacaaaaaat ttaccttctt tgttaacgaa aat
//
LOCUS       DAOQVQ010000030        10151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7418_length_10151_cov_4.593700, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000030 DAOQVQ010000000
VERSION     DAOQVQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_10151_cov_4.593700"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKN22_01815"
     CDS             complement(<1..569)
                     /locus_tag="PKN22_01815"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNS41468.1"
                     /translation="MKKILFTGIFLFFVTILSLAQNPANWTSAWGPSFRHLNAIEVLP
                     NNKIVVVGGWESNDAISSIFTTQDNGANWLTAMDNINAILQDVDFTDGNTGYAVGWAG
                     NILKTTNAGDNWSPITVTGNPGTRNFNGCYFFDNNTGVVVGGNKSNDAIQTILRTTDG
                     GSNWTVVSDNLAPWLSAVHFSNNSIGYAVG"
     gene            complement(742..2817)
                     /gene="paaZ"
                     /locus_tag="PKN22_01820"
     CDS             complement(742..2817)
                     /gene="paaZ"
                     /locus_tag="PKN22_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HNS41469.1"
                     /translation="MQRYKSYALGQWIDGEGVESNLYNAITGEHIGEVSSKGFNYTDI
                     LNYGRTTGRKVLSKMTFQERGRMLKALALYLLARKEKYYQISAWTGATRADSWVDIEG
                     GIGNLFANASLRKQFPDLPYYVDGDMAPLSKNGTFIGHHIMVPKEGVVVHINAFNFPI
                     WGMLEKIAVNLLAGVPTLVKPSSVTCFLTQVMVEDIIASKILPEGSLQLVLGSARELI
                     DSVTENDMVTFTGSHATGMKLKTSPTIVNNNVSFNLEADSLNACVLGEKAVPGTAEFD
                     LFVKEVVREMTTKCGQKCTAVRRMIVPEPLMDEVQKAVSARLSSVVIGDPAVEGVRMG
                     ALASHGQVKAVRESVEALNKSQEIVFGDLDNFAVVGADKNTGAFFSPILFRNDKPFEA
                     TDVHDIEAFGPVSTLMPYRSLDEAIELAKMGKGSLVSSIVTPNDKEAREYVVGAASMH
                     GRILVLNEQCAKESTGHGSPMPLLVHGGPGRAGGGEEMGGKRGVLHYLHRTAIQGHPT
                     TITEITQVYQQGAKGTISDTHPFKKYYEELSVGDQLITESRTITPEDIDQFAALSGDN
                     FYAHKRETNFEGTMFDEQVAHGYFIMSVAAGLFVDSYEINPVLLNYGIDELRFTKPVY
                     AGSNIHIRFTCKEKLPTETSPKADDPKSQIQRGIVKWLVEMLDDTNEPLVGIATILTM
                     VKTKDQTPR"
     gene            complement(2900..3805)
                     /gene="gldA"
                     /locus_tag="PKN22_01825"
     CDS             complement(2900..3805)
                     /gene="gldA"
                     /locus_tag="PKN22_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNS41470.1"
                     /translation="MSIEVKNLFKYYGEQAAVKDVSFTVNSGEIVGFLGPNGAGKSTT
                     MKIITGYIPASGGEVKVCGIPVDVDTLETRKKIGYLPENNPLYLDMYVKEYLEFVGNI
                     YKVKNVRARVEEMIKAVGLEVEQNKKIGMLSKGYRQRVGLAAAIIHDPEVLILDEPTS
                     GLDPNQLVEIRDLIKNIGKSKTVMLSTHIMQEVEAICDRVVIINKGQVVADNKASELQ
                     KQQTVQTVYVEFEDKLSKAQILKITSVQKVEQVKDGWLLESTAEVDLRRVVSKYAADQ
                     GWLTLTLKIEERSLEEVFKELTKGK"
     gene            complement(3857..6463)
                     /locus_tag="PKN22_01830"
     CDS             complement(3857..6463)
                     /locus_tag="PKN22_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNS41471.1"
                     /translation="MKKSYFLVALLLAVACGTKKTNSASVEATTSTDATVEQPATIVD
                     SASEEKVYHGSLTMLSDLIHTKLEVKPDWQKAYLNGVATITAKPHFYPSDSLILDAKG
                     MAINSVKLNGQTLTYTYTNDKLKIKLDKTYTRKDKYTVVIDYVAKPNERTTGGSAAIT
                     SDKGLYFINNDGQDKNKMPQIWTQGETEASSVWFPTIDATNQKTSQEIYITVDNKYVT
                     LSNGKLLSSKMNADGTRTDYWKQDLPHAPYLFMMGIGEFKVVKDYYTRKDGSKMDVFY
                     YVEPQWEKYARNIFGKTPEMISYFSKLLNYEYPWDKYHQIVVRDYVSGAMENTGAVVF
                     GDFAYKTDRELLDGTDESTIAHELFHHWFGDLVTCESWANLPLNESFANYSQYLWDEY
                     KYGIEEADLNAESEMKGYMQSAQAQGHHDLIWFDYDDKEEMFDGHSYNKGGRILHMLR
                     NYVGDDAFFTALNKYLTDNQYKSAEFHQLRIAFEQVTGEDLNWFFNQWFLASGHPTLE
                     YTQRTEGNEVVLTVKQKQDIKQFPVFKLPMQVAVVDNQGKHIHNITVTKVEEEFRFPV
                     AGNLVTLIPDYQEMLLAEVKETKPAAQFIQQYYHSTKWRTKNRALTLGVRKIDSLSAK
                     LVLDALKDPFWAIKSTAIGKASRLKDNDKATAIQLIKNLAQNDASSSVRAQAVKFLSD
                     EGVADMLAIYEKALADSSFTVNAAALNGMSESYPEQAMKKAEQLEKDESVSSSMLIAI
                     ATMYAKTGNPDKYEFFKTNLMNGKLNGFDALGFLSTFTGYLSGQSVEMADNALEIYTY
                     LEANGGMYTKMFLPQNLHYMSNILQSRISSETTKLEEYKKAKDTANAEKTQAAINQLK
                     AVQAKYAKMIEQ"
     gene            complement(6568..7161)
                     /locus_tag="PKN22_01835"
     CDS             complement(6568..7161)
                     /locus_tag="PKN22_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS41472.1"
                     /translation="MVRWKEKRKKIIGCVLFCMLSFASFGQKETYINPGLLSASATLS
                     PAIMLNRKEVNYYISGFFEGKITKHFSIRGDGYYLLGNAPTKFLKNKISAFIGFQYGY
                     PFGNLELYTGLAPGFTYMQSNVNTANKEFNPSVQLNAGVRYYVWKYFHFTANFLYTHA
                     QMHNLDRVNGMADELVFSVGLGFNFQALKKYRNPESN"
     gene            complement(7128..7766)
                     /locus_tag="PKN22_01840"
     CDS             complement(7128..7766)
                     /locus_tag="PKN22_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41473.1"
                     /translation="MRYFAVIAFCWLTAFGSHAQPLNIKKNQGGLFSLGVRSTSSFFG
                     DVNSFGFGGQMRLQFADRLNTEWFADFTRGNIQHRANRTDYHIGWSVMYYLTKKLAPA
                     VKPYILAGHCFDRTVIVDNSNKSHQIVKNSSAIQAGAGVHFNLTERLDITLIAQYMFH
                     LGKDVHAHIHDDGELDLSMHKGTGIEGHLLLNVGINYKIADLWYGGKKKERK"
     gene            complement(7946..8626)
                     /locus_tag="PKN22_01845"
     CDS             complement(7946..8626)
                     /locus_tag="PKN22_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS41474.1"
                     /translation="MKISYYGHSSLGLEINGKHILVDPFISQNPKAANIAIDSLKADY
                     VLLTHAHYDHIMDVEAIVERTKAKIISNHEIVTYYANNKQFEGHAMNQGGSWNFDFGR
                     VKVVNAIHSSSFPDGTYGGNPVGFVISAEGKNIYIAGDTALTMDMKLIPLFFKLELAI
                     LPIGGNFTMDIEEAIVAAQFVECDTIMGMHYDTAPYIEINHQEAHDKFKAINKNLLLL
                     NIGESYEL"
     gene            complement(8690..8920)
                     /locus_tag="PKN22_01850"
     CDS             complement(8690..8920)
                     /locus_tag="PKN22_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNS41475.1"
                     /translation="MIRVMQHRHKCIGCNACVEADKYRWRISKKDGKSTLIGATEKKG
                     WFSVVVDDDEESALEAAKVHCPVNIIRWERIG"
     gene            complement(8917..10143)
                     /locus_tag="PKN22_01855"
     CDS             complement(8917..10143)
                     /locus_tag="PKN22_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNS41476.1"
                     /translation="MELMAPAGSYEAIAAAVNAGANSIYFGVEQLNMRTKSTHNFTID
                     DLGKIAQICSENGLKSYLTLNTIIYDHDVQLMKRIIEAAKESNISAIIASDHAVMNYC
                     RKVGIPVHISTQTNVTNIDTVEFYSAYADVMVMSRELSLLQVHDINREIARREITGPS
                     GELVRIEVFGHGALCMAVSGKCYLSLHTNFSSANRGACIQNCRKSYIVMDKDDGQEFE
                     VDNEYIMSAKDLCTIDFLDKVIDAGVAVLKIEGRGRSADYVHVTTQCYREAIDAIYDG
                     TYTKEKIENWKERLSTVFNRGFWDGYYLGRKMGEWSDEAGSKATKRKVYIGKGMKYYE
                     NAGIGEFLIEAQGCEVGDELLIIGPTTGVVETVLKEMRVDNQETFAVKRGTTITFPVG
                     EKVRPSDKLYKLVPVQ"
BASE COUNT         2884 a         2121 c         1769 g         3377 t
ORIGIN      
        1 cctacagcat aacctattga gttattagaa aaatgaacag cactaagcca tggggctaag
       61 ttatccgaaa caaccgtcca atttgagccg ccatcagtag ttcttaatat tgtttgaatt
      121 gcatcattag atttgttacc accaaccaca actcctgtat tgttatcaaa gaaataacaa
      181 ccattaaaat ttctagttcc tggattacca gtaactgtaa ttggtgacca attatctcca
      241 gcatttgtag ttttcaaaat atttccagcc cagccaactg catatcctgt atttccatct
      301 gtaaaatcta catcttgtaa tattgcatta atattatcca tagctgttag ccaatttgca
      361 ccattatctt gtgtagtaaa aattgaggaa atggcatcat tcgactccca acctccaaca
      421 accacaatct tattattggg taatacttct atggcattaa gatgtctaaa cgaaggtccc
      481 caagcacttg tccaattggc agggttttga gctaacgata aaattgttac gaaaaacaaa
      541 aaaatcccag taaatagtat ttttttcata atgtgaataa taaataatag taaaactgtg
      601 agttaaaaag acaatacttt cattgtcaat gttataacaa atataaatat ataattttat
      661 taaaaaaaat tttctttcaa attttcataa cacaaaaaaa ccgcacttta acaatgcggt
      721 tttcgtttat tttatactca attaacgagg ggtttgatct tttgttttta ccatggtaag
      781 gatggtggca attccaacca atggttcgtt ggtatcgtcg agcatttcta ccaaccattt
      841 cacaatacca cgctgaattt gtgattttgg atcatcggct tttggtgatg tttcagttgg
      901 taatttttct ttgcacgtga aacgaatgtg aatgttggaa cctgcataaa ctggtttggt
      961 aaaacgcaat tcatctattc cgtagttaag caacaccggg ttgatttcat aactatcaac
     1021 aaacaaacct gctgccacac tcataataaa ataaccgtgt gccacttgct catcaaacat
     1081 tgtaccctca aaattggttt cgcgtttgtg tgcgtagaaa ttatcgccac tcaacgctgc
     1141 aaattgatct atgtcttctg gagtgatggt tctgctttct gtaatcaact gatcgccaac
     1201 gcttaattct tcgtaatatt ttttgaatgg atgggtatca gaaatagttc cttttgctcc
     1261 ttgttggtac acttgggtaa tttctgtaat ggttgttgga tgtccttgaa tggcagtgcg
     1321 gtgcaagtaa tgcaaaactc ctcgttttcc acccatttct tctccgccac ctgctctacc
     1381 cggaccacca tgaaccaaca atggcatagg cgaaccgtga ccagtgcttt cttttgcaca
     1441 ttgctcgttc aatactaaaa tacgtccgtg catgcttgct gctccaacaa cgtattcacg
     1501 cgcttctttg tcgtttggtg ttacaatgga tgaaaccaaa gatcctttac ccatttttgc
     1561 aagctctatg gcttcgtcta acgaacgata tggcatcaag gtagatactg gtccaaaagc
     1621 ttcaatatcg tgcacgtcgg ttgcttcaaa tggcttgtcg tttctaaata atattggaga
     1681 gaagaatgct cctgtgtttt tatcagcacc aaccactgca aagttatcca aatcaccaaa
     1741 aacaatttct tgcgatttat tcaatgcttc aacgctttcg cgcactgctt ttacttgtcc
     1801 gtgcgatgcc aaagccccca ttcttacacc ttctacagct gggtcgccaa ttacaaccga
     1861 gctcaaacgt gcagaaaccg ctttttgcac ctcatccatc aatggttctg gtactatcat
     1921 acgacgaacg gccgtacatt tttgaccaca tttagtcgtc atttcgcgca ccacttcttt
     1981 aacaaataaa tcaaactctg ctgtacccgg aacggctttt tcgcccaata cacatgcgtt
     2041 taacgaatcg gcttctaggt tgaatgacac gttattgtta acgatggttg gacttgtttt
     2101 taatttcatt cccgtagcat ggcttcccgt aaaagttacc atgtcgtttt cggtaacgct
     2161 atcaattagt tcgcgagcgg agcccaacac caattgcaac gatccttctg gtaaaatttt
     2221 agaagcaata atatcttcta ccatcacttg tgtcaagaaa cacgtaacgc ttgatggttt
     2281 taccaaggtt ggaactcctg ccaataagtt aacggcaatt ttttccaaca ttccccaaat
     2341 cgggaagtta aatgcattga tgtgcaccac tacaccttct tttggaacca tgatgtggtg
     2401 accgataaat gttccgtttt tagacaacgg tgccatgtcg ccatccacat aatacggcaa
     2461 atctgggaat tgtttacgca acgatgcatt agcaaataaa tttccaattc caccttcaat
     2521 atccacccaa gaatcggcac gtgtggcacc tgtccatgca ctaatttggt aatatttttc
     2581 tttgcgcgcc aaaaggtata aggccaatgc ttttagcata cgaccacgct cttggaacgt
     2641 cattttactc aacactttgc ggcctgttgt gcgaccgtag tttaaaatat cggtgtaatt
     2701 aaatcctttg ctggaaactt ctccaatgtg ttcacccgta atggcgttgt ataaattgct
     2761 ttctacacct tctccgtcta tccattgacc gagtgcataa cttttgtatc gttgcatggt
     2821 tttacttttt attttaacaa agcgttaaag ttaattataa aatgtataaa ttctttgagc
     2881 actgcaaaga attaacggtt tattttcctt tggttaattc tttaaagact tcttccaatg
     2941 agcgttcttc aatttttagc gttagtgtta accacccttg atcggcggcg tatttcgaca
     3001 ctactcgtct taaatctacc tcggcagttg attccaataa ccaaccatct tttacttgtt
     3061 caactttttg tacggaagta atttttaaga tttgtgcttt gcttaatttg tcttcaaact
     3121 ccacataaac cgtttgtact gtttgttgtt tttgcaattc ggatgcttta ttatcggcta
     3181 caacttgccc tttgttgatg ataactacac gatcgcaaat ggcttccacc tcttgcatga
     3241 tgtgtgtgga aagcataacc gttttacttt ttccgatgtt tttaatcaaa tcgcggattt
     3301 caaccaattg gttagggtcg agaccagatg taggttcatc caaaatcaac acttctgggt
     3361 cgtgaataat tgctgcggcc aacccaacac gctgacgata accttttgaa agcatgccta
     3421 tttttttgtt ttgctctact tccaaaccta cggctttaat catttcttcc acacgagcac
     3481 gtacgttttt tactttgtaa atgttgccaa caaattccaa gtattcttta acgtacatat
     3541 ccaagtagag cgggttattt tcgggtaaat agccgatttt tttacgagtt tcaagggtat
     3601 ccacatcaac aggaataccg caaactttta cctcaccacc acttgcggga atatagcctg
     3661 taatgatttt cattgttgtt gattttccgg caccatttgg acctaagaaa ccaacaattt
     3721 caccagaatt aacggtgaac gaaacgtctt ttactgccgc ttgttcaccg taatatttga
     3781 ataagttttt tacttcaata gacattttca attctttgat agcacgaaga tacgcaaaga
     3841 aaaaaaccta cgccgattat tgctctatca ttttagcata ttttgcttga actgctttca
     3901 attggttgat tgcagcttgc gttttttcgg catttgctgt gtctttagct tttttgtatt
     3961 cttccaactt tgttgtttcg ctactaatgc gtgattgtaa tatattggac atgtagtgca
     4021 agttttgagg caagaacatt ttggtgtaca taccgccatt tgcttccaaa taggtgtaaa
     4081 tttccaatgc gttatcggcc atttctacac tttgacccga taaataacca gtaaatgtgc
     4141 tcaaaaagcc caaagcatca aaaccattta attttccgtt cattaagtta gttttgaaaa
     4201 actcgtactt atcaggattt cctgttttag catacatggt ggcaattgca atcaacatag
     4261 aagaagaaac gctttcatct ttttccaatt gttcagcttt tttcattgct tgttcaggat
     4321 agctttcact cattccgttc aaagcagctg cattaactgt aaacgaagaa tctgcgagtg
     4381 ctttttcgta aatagccaac atatctgcca caccttcatc gctaaggaat ttaacggctt
     4441 gtgcacgaac agaggatgaa gcgtcatttt gagctaagtt tttaataagt tgaatagccg
     4501 ttgctttatc gttgtctttt aagcgacttg ctttaccgat ggctgttgat tttattgccc
     4561 aaaaaggatc tttcaacgca tctaacacca atttagcaga taaactgtca atttttctaa
     4621 cacccaaagt aagtgctctg tttttggttc tccatttggt agaatgataa tattgttgaa
     4681 taaattgcgc agcaggtttg gtttctttca cttctgccaa caacatttct tggtaatctg
     4741 gaatgagggt tactaaattt cctgcaactg ggaaacggaa ttcttcctcc acttttgtta
     4801 ccgtaatgtt gtgtatgtgt tttccttgat tgtcaaccac agcaacttgc attggcaact
     4861 taaatacagg gaactgtttt atatcttgtt tttgtttcac ggttaacacc acttcgttgc
     4921 cctctgtgcg ttgggtgtat tctaatgttg ggtgacctga agctaaaaac cattgattga
     4981 aaaaccagtt taagtcttca cctgtaactt gctcaaaagc aattcttaat tggtgaaatt
     5041 ctgccgattt gtattgattg tcggtcaaat atttgttcaa agctgtaaaa aacgcatcgt
     5101 ctcctacata attgcgcaac atgtgtaaaa tacgccctcc tttgttgtac gaatggccat
     5161 caaacatttc ttctttatca tcataatcaa accaaattaa atcgtggtga ccttgtgctt
     5221 gagcagattg catataacct ttcatttcgg attcggcatt taagtctgct tcttctatac
     5281 catatttgta ctcgtcccac aagtattgcg aataattagc aaacgattcg ttgagtggta
     5341 aattggccca cgattcacag gtaaccaagt cgccaaacca gtggtgaaac aactcgtgag
     5401 caatggttga ttcatctgta ccatccaata attcacggtc tgttttgtag gcaaaatcac
     5461 caaaaacaac tgctcccgtg ttttccattg ctccagaaac atagtcgcga acaacaattt
     5521 ggtggtattt atcccatgga tattcatagt tcaacaattt tgagaaatag ctaatcattt
     5581 ctggtgtttt tccaaaaata ttgcgtgcat atttttccca ttgaggttct acataataga
     5641 aaacatccat tttactaccg tctttgcgag tgtagtaatc tttaaccact ttaaactcac
     5701 caattcccat cataaacaag tagggagcat gtggtaaatc ttgcttccaa tagtcggtgc
     5761 gggttccatc ggcattcatt ttactgctta gcaacttacc atttgacagg gtaacatatt
     5821 tattatcaac agtaatgtaa atttcttgcg atgttttttg attggttgca tcgattgttg
     5881 gaaaccacac agaactagct tctgtttctc cttgcgtcca aatttgaggc attttgtttt
     5941 tatcttgccc atcgttgttg atgaaataca aacctttatc cgaagtgatg gctgcacttc
     6001 cgcctgttgt acgctcattt ggtttagcaa cataatcaat cacaacggtg tatttatctt
     6061 tgcgggtata ggttttatcc aacttgattt tcaacttatc gttggtatag gtgtatgtga
     6121 gtgtttgacc gttcaatttc accgaattga ttgccatacc tttagcatct aaaatcaaac
     6181 tatccgaagg ataaaagtgt ggttttgcgg taatggttgc tactccattc aaataggctt
     6241 tttgccaatc gggttttact tccaacttgg tgtgtatcaa atccgacagc attgtaagtg
     6301 agccgtgata aactttttct tctgaggcag aatcaacgat tgttgctggt tgttcaacag
     6361 tagcatcggt tgatgtagtt gcttctactg atgctgagtt tgtttttttt gttccacaag
     6421 caaccgcaag aagtagtgct actaaaaaat aagacttttt cattttacgt attttgtaca
     6481 aaaatgcaaa atatcttcca acaacgcttt aaaaagtgat aaatggttac ttggttgcta
     6541 ccaacgtcaa aaattcgtga taaattgtta attgctttct ggattgcggt attttttgag
     6601 ggcttgaaaa ttaaacccta atccaactga aaacaccaat tcatcggcca ttccgttaac
     6661 tctatccaag ttgtgcattt gtgcgtgggt gtacaagaaa tttgctgtga aatggaagta
     6721 tttccacaca taatagcgca cacccgcatt gagttgcacc gaaggattaa actctttatt
     6781 ggcggtgttt acattggact gcatgtaggt aaaaccaggt gccaatcccg tataaagttc
     6841 caaattacca aagggataac cgtattgaaa accaatgaat gcacttattt tgtttttcaa
     6901 aaacttggtt ggggcatttc ccaataggta ataaccatcg ccacgaatgc taaaatgttt
     6961 agtgatttta ccttcaaaaa atccgctaat gtagtaattc acctctttgc ggttaagcat
     7021 tattgcaggt gaaagcgtgg cagatgcact taacaaacca gggttgatat aggtttcttt
     7081 ttgtccaaaa gaggcaaaac tcagcatgca aaacagcaca caaccaatta ttttctttct
     7141 tttttctttc caccgtacca taagtcagcg attttatagt taatacctac attcaacagt
     7201 aaatgtcctt ctattccagt tcctttgtgc attgacaaat cgagttctcc atcgtcgtgt
     7261 atatgcgcat gtacatcttt tccgaggtga aacatgtatt gggcaatgag ggtgatatcc
     7321 aaccgttcag ttaagttaaa atgcacaccc gcacccgctt gaatagccga gctgtttttt
     7381 acaatttggt gcgatttgtt ggaattatcc acaataacag ttctatcaaa acaatgacct
     7441 gcaagaatgt agggttttac agctggtgct aatttttttg ttaaataata catcacactc
     7501 caacctatgt ggtaatctgt acgattagca cgatgttgta tgtttccacg tgtaaaatcg
     7561 gcaaaccatt cggtgttgag gcgatcggca aattgcaaac gcatttgacc accaaaacca
     7621 aaggaattga catcgccaaa aaaactagaa gttgaacgaa cacccaatga aaacaaacca
     7681 ccttgatttt ttttgatgtt caacggttgc gcatggctcc caaaagctgt aagccaacaa
     7741 aacgcaataa cagcaaagta cctcatagtc aatagatttt aaagttgatg gttttcataa
     7801 gtacgacaaa agaagtgaat atattgcaat aaatggttaa aaaattaaat ttttgattga
     7861 aaattaacct cttagccgtt cttttaaaca aaaaatgcac tagaaaattc taatgcattt
     7921 ttatgataat ctatttattc aaatcttaca attcgtagct ttctccaatg ttcaacagca
     7981 acaagttttt attgatggct ttaaatttat cgtgtgcttc ttggtgattg atttcaatgt
     8041 agggcgctgt gtcgtagtgc attcccataa tggtgtcgca ttcaacaaat tgagcagcaa
     8101 ctattgcttc ttcaatatcc atggtgaaat ttccgccaat aggcaaaata gctaattcca
     8161 atttaaaaaa tagtggaatt agtttcatat ccattgttag tgcagtatca cccgcaatgt
     8221 agatgttttt tccttcggca ctaataacaa atccaacagg attgccgcca taggttccat
     8281 ctggaaatga agaagaatgg atggcattaa ccacttttac acgaccaaaa tcaaaattcc
     8341 acgaacctcc ttggttcatt gcatggcctt caaattgctt gttattggca taatacgtta
     8401 caatttcgtg gttggaaatg attttagctt tggtacgttc aacaatggct tcaacgtcca
     8461 taatatggtc gtaatgtgca tgtgttagca atacgtaatc tgcttttaat gaatcaattg
     8521 caatattagc tgctttgggg ttttggctga taaaaggatc aaccaatatg tgttttccat
     8581 taatttctaa tcctaatgac gaatgtccgt aatacgatat tttcatagtg aattgaattt
     8641 ttttttaaaa ttagtaaaaa attttacgtt ccacatgaaa ccagtaaaat taccctattc
     8701 tttcccaacg aatgatattg acaggacaat gcactttggc tgcttctaat gcactttctt
     8761 cgtcgtcgtc cacaacaaca ctaaaccaac cctttttttc agttgctcca ataagcgtgc
     8821 tttttccatc ttttttggaa atgcgccatc ggtatttgtc ggcttctaca caagcattac
     8881 aaccaatgca tttatggcgg tgttgcatta cacgaatcat tgtacaggca ctaatttata
     8941 gagtttatca cttgggcgta ccttctctcc tactgggaaa gtaatggttg ttccacgttt
     9001 aaccgcaaaa gtttcttggt tatccacacg catttctttc aaaacggttt ctaccacacc
     9061 ggtagttgga ccgataataa gcaattcatc gcctacttcg cagccttgcg cttcaattaa
     9121 gaactcgcct atacctgcat tttcgtagta tttcattcct tttccgatat acaccttgcg
     9181 ttttgtggct tttgaaccag cttcatcgct ccattcgccc atttttctac ccaagtagta
     9241 accatcccag aatccacggt taaacactgt actgaggcgt tctttccagt tttcaatttt
     9301 ttctttggta taggttccat cgtaaatagc atcgatagct tcacggtaac attgtgttgt
     9361 aacatgcacg taatcagcac ttcttccacg accttctatt ttcaacaccg ctactccagc
     9421 atcaataact ttgtctaaaa agtcaatcgt acacaaatct tttgccgaca tgatgtattc
     9481 attatccact tcaaattctt gtccatcgtc tttgtccata acgatgtagc tttttcggca
     9541 attttggatg catgctccac gattagccga tgaaaaatta gtgtgtaagc tcaaataaca
     9601 tttgcccgaa acagccatgc acaaggctcc gtgaccaaaa acttcgatgc gcactaattc
     9661 accagaaggt ccagtaattt ctcttcgggc aatttcacga ttaatgtcgt gcacttgcaa
     9721 caagctcaat tcgcgcgaca tcaccataac gtctgcatat gccgaataaa actctaccgt
     9781 atcaatattc gtaacatttg tttgagtaga aatgtgcacc ggaataccca cttttctaca
     9841 ataattcatt actgcgtggt cggaagcaat aatggcagaa atattacttt ccttagctgc
     9901 ttcgatgatg cgtttcatca attgcacatc gtgatcgtag atgatggtat tgagcgttaa
     9961 gtaacttttc aatccatttt cactacaaat ttgtgcgatt tttcctaaat catcaattgt
    10021 aaagttgtga gttgatttgg tgcgcatgtt gagttgttcc acaccaaaat aaatagagtt
    10081 tgctcctgcg tttacggctg cagcaatagc ctcataggaa cccgcaggcg ccattagttc
    10141 aatcttttgt t
//
LOCUS       DAOQVQ010000031         9779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7779_length_9779_cov_4.124743, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000031 DAOQVQ010000000
VERSION     DAOQVQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7779_length_9779_cov_4.124743"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2005)
                     /locus_tag="PKN22_01860"
     CDS             complement(<1..2005)
                     /locus_tag="PKN22_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41477.1"
                     /translation="MMKLLSTFILFIAFFAVNTFSQNLVINPSFELNSGCPMGPSELS
                     KANNWSDPFVNLVGDTCSTSDYYHSCSPLGAMGVGVPANILGNEPARTGSAYAGIITY
                     SGMALTGCTPMMSDGWREYLQGQLTTPLTAGQQYCVSFWVSLADNVKWASDLWGVHLS
                     PTPVAVNCATVGSNSDLASYGIVPQLTYNGPAITSTNGWTKIQFTYTATGGEQYFVIG
                     NFYNSATTYTCVNASAMNPYAYYYVDDVSITDGVCCDATFTAPTTMCNTDAAVTLTPS
                     TAGGTWSGNGVNSSGVFDPAIAGVGAHTITYTLPCGSSENHTITVNSCVSLSVCKEAN
                     GSITVSGGTAPYSWQNQTTTQDCSACLVGCTFPPGCAVNVTTWTTFGTGTNQTPGTYP
                     VQIVDNSGGTILITSAASVTACSADPCASVTITTTITPTQPTCTAAGSAIANPTGGTA
                     PYSYSWNTSPVQTTQTATNLSAGNYTVTVTDNNGCTGTQNVTINAVPNAPTVTITPTQ
                     PTCTVAGSAIANPTGGTAPYSYSWNTTPVQTTQTATNLSAGNYTVTVTDNNGCTGTQN
                     VTINAAPSSPTVTTTTTDADCSGTLGTATANVTGGTAPYSYSWNTSPVQTTQTATNLS
                     AGNYTVTVTDNSGCIATTNAAINTSGGTITIQSITPTQPSCAGA"
     gene            complement(2125..3138)
                     /locus_tag="PKN22_01865"
     CDS             complement(2125..3138)
                     /locus_tag="PKN22_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNS41478.1"
                     /translation="MPFIKNTLFRHRIIDKCLRNPYKPFPSKETLRIACEQALFGSED
                     KQNICDSTIEKDLFAMRQEYDAPIKYSKLHRGYYYEDPNFTINDVPLNESDLDAVRFA
                     ANTLLQFKDVEMFKEFGNAIDKIVERITLSSNAKHQPELTEFVQFETAYSVGGGEYLP
                     SLLEAIQKSVAVYFEYENFNSKTVKSRKVTPLLLKEYRNRWYLISYDLVKDKITTYAL
                     ERIKKLELSSEKVVKPHYFQPKEFFNYAIGISTKDTAPEIVVFEADDVASKYLIAQPL
                     HHSQKIVAQTENTVTFQLEVYISEELIRSLLSFGGEINVKAPQILVNDMKTRVAQMMK
                     FYK"
     gene            3278..4507
                     /locus_tag="PKN22_01870"
     CDS             3278..4507
                     /locus_tag="PKN22_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS41479.1"
                     /translation="MQSLYNALTYAKENSPFYREQLAHFHFENNEQIVEQLHALPFTT
                     KDDLAQRNRDFLATSKHKIAEYVTTSSTAGSPVTIFLTKNDLQRLAFNEKVALSCTGA
                     NEEDIFQLLTTIDRQFMAGLAYYLGVQEMGAGMVRIGPGVPALQWKSIFDNEVTVLIA
                     VPSFIITLIAYAKMNNIDYKQSKVKKIVCIGEALREDDFSLNVLGKRIQQDWDVELYS
                     TYASTEMGAAFTECLAQNGGHLNPDLIHLEVIKEDGSHAQNNEIGEVVVTTLGVEGTP
                     LIRYKTGDLARLHTTTCACGRTTPRLGPIIGRKNQLIKYKGTTIFPNAIYEVFDQQPA
                     VTCYKVEISKDDLGNDMITVLLGKKIENTTEMQLIIADCQAKLKVVPHFVFLEEDYLR
                     SQVFKKNSRKPEKIVFK"
     gene            complement(4853..5758)
                     /locus_tag="PKN22_01875"
     CDS             complement(4853..5758)
                     /locus_tag="PKN22_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41480.1"
                     /translation="MKLNEKDNIKDLFQEQLGNLESPVNPELWQSIASQVGITATTST
                     VTATGISLAAKLAIGISAASVIAVGAYFITKDTSTSVATNKTIIVENQKPSTETSEVE
                     TSQQNDGVKKLDVQPTHQPAITPNSTKNNSNLVEEVKNTTTSSDDLLLDNKTYISTPN
                     PNIMDKKSATTTNENKVVAQEPAPTKTENKTNEVVNKGTTSNAVANDAQPTFELITLP
                     NIYSLSANGYFSIDYKGEYSDFQFTLMDENSNVIFKSAQPDFKWRGTDSFNNVIQPGK
                     YIYIITVKDVNGKAINKFSTLTVIN"
     gene            complement(5736..6278)
                     /locus_tag="PKN22_01880"
     CDS             complement(5736..6278)
                     /locus_tag="PKN22_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS41481.1"
                     /translation="MEEQTLIKDCLNGNPIAQRKLFEKFAPKMMTVCLRYMKDRQEAE
                     DVLQDGFVKVFQKFHLYENSGSLEGWIRRIIVNTALDQLRKNSKFGYMDDVDEVGYKI
                     ENNSYTFENLVAQDLMKMIDQMPNGYKVVFNMFAIEGYSHKEIGEMLGISENTSKSQY
                     LRARDYLKKQLEKIEIEREG"
     gene            6394..7620
                     /locus_tag="PKN22_01885"
     CDS             6394..7620
                     /locus_tag="PKN22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS41482.1"
                     /translation="MKFILFSIFFVSSYFSFAKPTIRIKSGKWIGVLELTQNEKLYFE
                     IRIDNKKSNCTIQIKNGEEIVPLNQPTINGDSVHVQFANFNTELIFKVVNKTSIQGRW
                     VNHIKTNYEIPFKAYLSKATTVFPITKLSPVKNFDGRWKSSFKDMHGEEIAIGLFQQN
                     GANATGTFLTETGDYRFLSGNVDSNTLYLSGFDGSHAYLFVGTQDDQGKIKGLFLSGT
                     HHRSVWTAERNDTFQLSDADSITTFVGESKDDIILNFKNLDGTNFEYPNEQFKDKVVI
                     IQIMGTWCANCMDETVYFKELYDKYNAKGLEIITVGYETGATFEECSKQLQAFQQRFN
                     LKNTIVVGGNVRKNKVMDDFTFLSNFSSYPTSIFIDKKGNVARIHTGFSGPSTGKIYT
                     DYKQETERLIEELINL"
     gene            7668..8180
                     /locus_tag="PKN22_01890"
     CDS             7668..8180
                     /locus_tag="PKN22_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNS41483.1"
                     /translation="MKKAIIWISILFFLPSCGGNDTEFKDLRGKEVESTIAATPLSQK
                     ELDESLAKIRAEEEAKEAKERSMQTTLSFDKTEHDFGNVKAQTPNEASFKVTNTGDKP
                     LIISNVETSCGCTNSKKPEKPILPGQSDEIAVTFKSNPGQTGEQHKTITVSANTKEKT
                     HQLKIRAFVK"
     gene            complement(8173..9072)
                     /locus_tag="PKN22_01895"
     CDS             complement(8173..9072)
                     /locus_tag="PKN22_01895"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HNS41484.1"
                     /translation="MEKQQFSAAGKLLFFGEYLVLRGSKCLSFPLKYQQTLTVSSQQL
                     PEIHWTCFDENAVCWLDIRFSSSLEIIQTSDEEKAKIAQRLLQLICEKNPTLKCAGLN
                     LEFSLNFNRMFGFGTSSTLLSLLSQWSGVDAYYLLEHSFGGSGYDLATALSRQPIVYT
                     MRDKTLQNVQLSPSISNQLIFVYLGAKQSSANEIANFKNKITTTAHINAMNAIVEQAT
                     VCTTIEEWEKLMNASEQLLSTILAIAPIQQRLFSDYPHCIKSLGAWGGDFIMATCSNK
                     SQATAYFREKGYNIIYCYDDLII"
     gene            complement(9086..>9779)
                     /locus_tag="PKN22_01900"
     CDS             complement(9086..>9779)
                     /locus_tag="PKN22_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNS41485.1"
                     /translation="DGLEIIMCILSNLTPNCRVRCTVETPINEFDNIVPGISGKEFAR
                     RFRLGVRVAEVDPHRATTHNKGIMNGVDSVVLATGNDFRAVEACAHTYAAKDGKYTSL
                     SHVELNGDLFRFEMEIPFALGVVGGLTSLHPLVKLSLELLDNPNAKELMAIAATIGMA
                     NNFSAVKNLVTVGIQQGHMKMHLLNIIKQLNIEEVHRNAIVEHFKDKVVSVSAVRNLA
                     EKWNLIPAEKVK"
BASE COUNT         3105 a         1731 c         1755 g         3188 t
ORIGIN      
        1 atgcacctgc acatgaaggt tgtgttgggg ttatgctttg tatagtaatt gtaccaccac
       61 ttgtgtttat agctgcattg gtagttgcta tacatccact attatctgtt actgttacag
      121 tatagttacc tgcacttaaa ttggttgcag tttgtgtagt ttgcactggc gatgtattcc
      181 aactataaga gtaaggtgct gttcctcctg taacatttgc agttgctgtt cctaaagttc
      241 cactacaatc tgcatctgtt gttgttgttg taactgtagg tgaagacgga gcagcattaa
      301 tagttacatt ttgagttcct gtacatccat tattatctgt tactgttacc gtatagttac
      361 cagcacttaa attggttgca gtttgtgttg tttgcactgg tgttgtattc caactgtatg
      421 aataaggagc tgttccacct gttggatttg caattgccga accagcaaca gtacaagttg
      481 gttgtgttgg tgtgatagtt acagttggtg catttggaac ggcattaata gttacgtttt
      541 gagttcctgt acacccgtta ttatctgtta ccgttaccgt atagttacct gcacttaaat
      601 tagttgcagt ttgtgttgtt tgcactggtg atgtattcca actgtatgaa taaggagctg
      661 taccacctgt tggatttgca attgccgaac cagcagcagt acaagttggt tgtgttggtg
      721 ttattgttgt agtgattgtt accgaagcac aaggatctgc actacaagca gtaactgaag
      781 ccgcactagt tattaaaata gttccacctg aattatctac aatttgtaca ggataagtac
      841 caggtgtttg gtttgttcca gtgccaaaag ttgtccatgt tgtaacattt accgcacaac
      901 caggaggaaa cgtacaacca accaagcaag cactacaatc ttgagtagtt gtttgatttt
      961 gccatgaata aggagctgtt ccaccagata cagttatact tccattagct tctttacaca
     1021 cactcaagct cacgcagctg ttaactgtaa tagtatggtt ttcagagcta ccacaaggca
     1081 gtgtgtaggt aatagtatgg gcgccaactc cagcgatagc ggggtcaaaa acaccagaag
     1141 aatttacgcc attacccgac caagttcctc cagctgttga aggggttaat gtaactgctg
     1201 catctgtatt acacattgtt gttggggctg tgaaagtagc atcacaacat acgccatcag
     1261 ttatacttac atcatccaca taataataag cataaggatt catggcgctt gcatttacgc
     1321 atgtgtaggt ggttgcactg ttataaaaat ttcctataac aaaatattgt tcaccaccag
     1381 ttgctgtata ggtaaattgg atttttgtcc acccattagt agatgtaatt gctggcccat
     1441 tatatgttag ttggggcaca attccataag atgctaaatc ggaattactt cctacagtag
     1501 cacagttgac tgcaacagga gttggagata aatgaactcc ccataaatca cttgcccatt
     1561 ttacattgtc ggccaagcta acccaaaaag aaacgcaata ttgttgtcca gctgtaagtg
     1621 gtgttgttag ctgtccttgt aaatattctc tccatccatc actcatcatt ggtgtacacc
     1681 ctgttaatgc catacccgaa tatgtgataa taccagcata agcacttcct gttcgtgcag
     1741 gctcatttcc caaaatattt gcgggaacac caacccccat tgcacctaat ggactacaag
     1801 agtgataata atcagatgtt gaacatgtgt cacctaccaa attaacaaat gggtctgacc
     1861 agttatttgc ttttgataat tcacttggtc ccatcggaca tccactattt aattcaaaac
     1921 tgggattgat tactaaattt tgagaaaatg tgtttacagc aaaaaaagca ataaacagaa
     1981 taaatgtaga tagtagtttc atcatagttc tttgtaatag caatacttaa ttcatacaat
     2041 attaataaat tataccaatt aaagattggt ttgttgattt attaacaaac aatgagaatt
     2101 gatttatgaa aaataacagt ttgattattt ataaaacttc atcatctgtg caaccctcgt
     2161 tttcatgtcg ttaactaaaa tctgtggtgc ttttacattt atttcaccgc caaatgatag
     2221 taaggagcgt attaattctt cagaaatata gacttccaat tgaaaagtaa ccgtgttttc
     2281 tgtttgagcc acaatttttt gactgtggtg caagggttga gcaattaaat atttggaagc
     2341 aacatcatcg gcttcaaaaa caacaatttc tggagcggta tctttggtag aaattccaat
     2401 cgcataatta aaaaattctt tgggctggaa ataatgaggt ttaaccactt tctcggaaga
     2461 taattccaat ttttttatac gttccaaggc ataggtggtg attttgtctt tcaccaaatc
     2521 ataggaaatc aaataccaac gattgcggta ttctttgagt agtaaaggag taactttgcg
     2581 cgatttaacg gtttttgaat taaaattttc gtattcaaaa taaacggcaa cacttttttg
     2641 aatggcttct aataatgagg gtaaatattc tccaccacca acagaataag cagtttcaaa
     2701 ttgaacgaat tcggttaatt cgggttggtg tttggcattt gaactcaagg taatacgttc
     2761 cacaattttg tcgatggcat taccaaattc cttaaacatt tccacatctt tgaattgcaa
     2821 caatgtattg gccgcaaacc ttaccgcatc caaatcggat tcgttgagtg gaacatcatt
     2881 tatagtaaaa ttggggtctt cgtaataata acctcggtgc aatttgctgt atttgatggg
     2941 tgcatcgtat tcttgacgca tagcaaataa atccttttcg atggttgaat cgcaaatatt
     3001 ttgtttgtct tcacttccaa acaacgcttg ttcgcacgca attctcaacg tttctttaga
     3061 gggaaacggc ttgtagggat tgcgcaaaca tttgtcaata atccgatggc gaaaaagcgt
     3121 gtttttaatg aaaggcatac tttattttta tacagctgat tatcaaaaat aataaacata
     3181 ttgtgtaaag tgtgattttg attgattttt gtcgtaaaaa tgggtttttt tataaaatct
     3241 gctaattttg agtttttaaa tagataaaac attctcaatg caatcactat acaatgcgct
     3301 tacatacgca aaagaaaatt ccccttttta tagagaacaa ttagcgcatt ttcattttga
     3361 aaataatgaa caaattgttg aacaattgca tgcattgcct tttactacca aagacgactt
     3421 ggcacaacgc aaccgtgatt ttttagcaac ctctaagcat aaaattgcag aatacgttac
     3481 aacatctagt accgctggtt caccagttac aattttcctt accaaaaacg atttgcagcg
     3541 attggcattc aacgaaaaag tagctttaag ttgcaccgga gcgaatgaag aggatatttt
     3601 tcaattgctc acaaccatcg atcgtcagtt tatggctgga ttggcctatt atcttggtgt
     3661 gcaagagatg ggtgctggta tggtcagaat tggccctggc gttcccgcat tgcaatggaa
     3721 atctattttt gataacgaag ttactgtttt aattgctgta ccaagtttta ttattacgtt
     3781 gattgcttat gcaaaaatga ataatattga ttacaagcaa tcgaaagtta aaaaaattgt
     3841 gtgcattggc gaagcattgc gcgaagacga ttttagtttg aatgtgcttg gaaaacgcat
     3901 ccaacaagat tgggatgtgg aactgtattc cacctacgcc tctactgaaa tgggagcagc
     3961 ttttacggag tgtttagctc aaaatggtgg acatttaaac cccgatttga ttcaccttga
     4021 agtgattaaa gaggatggga gtcacgctca aaataacgaa ataggagagg tggttgttac
     4081 aactttggga gtagaaggaa cgccattaat tcgctataaa acaggagatt tagcacgatt
     4141 gcatacaaca acatgtgctt gcggacgaac aacacctcgt ttaggtccta ttattggccg
     4201 taaaaatcaa ttaattaaat acaaaggcac aactattttt ccaaacgcca tttatgaagt
     4261 gtttgatcaa caaccggctg taacgtgtta caaagtagaa atttcaaaag atgatttagg
     4321 aaacgatatg attaccgtgt tgttgggaaa gaaaatagaa aatacaacag aaatgcaact
     4381 gataattgca gattgtcaag caaaactaaa agttgtacca cattttgttt tcttagaaga
     4441 agattatttg cgtagtcaag tatttaagaa aaacagtaga aaaccagaaa aaattgtgtt
     4501 taaataaaaa agaccgcatt aaaacggtct ttttaaactc acctaattac ctaaccttac
     4561 ctaaaataaa atctatcaac tattattatt tattactcga accagttttg ttagattgcc
     4621 atttcttttt caaatgctgt gccaaaacat aatatttttt caaaacattg aaaaacaaag
     4681 cattacgatt tttataaaat tattgagggt taaaaatatt ttcaaaatga aaaaatagca
     4741 ttcaaaatga atggaaaaat ggtgtttaca tcctatttct agagttcaat ataaaattat
     4801 tcaaacgaca taaaatcgtt caatttcgaa atcaataaac aaaaggataa gattaattga
     4861 taacagtcaa cgtgctgaat ttgttgattg ccttgccgtt tacatcttta acggtaatga
     4921 tgtagatgta ttttcctggt tgaataacgt tgttaaacga gtcggttcct ctccatttaa
     4981 aatcaggttg tgcacttttg aaaatcacat tgctgttttc atccattaat gtgaattgga
     5041 aatcgctgta ttcacccttg taatcaatag aaaaataacc gttggcactt aatgaataaa
     5101 tatttggtaa ggtaattaac tcaaatgtag gctgtgcatc atttgcaact gcatttgatg
     5161 ttgtcccttt attaacgact tcgtttgttt tgttttctgt ttttgttgga gcaggttctt
     5221 gagcaaccac tttattttcg ttggtggtag ttgccgattt tttatccatt atatttggat
     5281 taggcgtact gatatacgtt ttattgtcca acaataaatc atctgatgaa gtagtagtgt
     5341 tttttacttc ttcaactaaa ttagaattat ttttagttga atttggagtt attgccggtt
     5401 gatgtgttgg ttgaacatct aattttttaa ccccatcatt ttgttgactt gtttcaactt
     5461 ctgatgtttc agtagatggt ttttgatttt ctactattat agttttattt gttgctacag
     5521 aagttgaagt atcttttgta atgaaatagg cacccaccgc aatcacagat gcagcagaaa
     5581 taccaattgc taacttagct gcaagagata ttcctgttgc tgtgacagta gaagtagtag
     5641 cagtaatgcc tacttgagaa gcaatagatt gccataattc aggatttact ggcgattcta
     5701 agttccccag ttgttcttga aacaaatctt ttatgttatc cttctcgttc aatttcaatt
     5761 ttttctaatt gtttttttaa ataatcgcgt gctcttaaat attgcgattt ggaggtgttt
     5821 tcagaaattc caagcatttc accgatttct ttgtgcgaat aaccttcaat tgcaaacata
     5881 ttgaatacca ctttgtatcc attgggcatt tgatctatca ttttcatcaa atcttgcgcc
     5941 accagatttt caaatgtata actattgttt tcaattttat atcccacttc atccacatca
     6001 tccatgtacc caaattttga gttttttcgt aattgatcca gagcagtgtt aacaataatt
     6061 cttcttatcc aaccttccaa tgatcctgag ttttcgtata aatgaaattt ctgaaatact
     6121 ttcacaaatc catcttgcaa aacatcctct gcttcttgtc ggtctttcat ataccgcaag
     6181 caaacagtca tcatttttgg cgcaaatttt tcaaacaact ttcgttgagc aatggggttg
     6241 ccatttaaac aatcttttat taatgtttgc tcctccattt actcccaaca atcttataga
     6301 ctttatttgt ttaattaaag ttgcatcact tgcaacaaaa aaataatttt taccgaaata
     6361 tacttatatt tagcaactca aaacaacact ttaatgaaat ttatactttt ttcaatcttc
     6421 tttgtttctt cttacttttc ctttgcgaaa ccaactattc gtattaaaag tggtaagtgg
     6481 ataggtgtgt tagagctgac tcaaaacgaa aaattgtatt ttgaaataag gatagataat
     6541 aaaaaatcaa attgtacaat tcaaattaaa aacggagaag aaatagttcc gctaaaccaa
     6601 cctacaataa atggcgatag tgttcatgtg caatttgcaa attttaatac agaactaata
     6661 tttaaggtag tcaacaaaac ttcaattcaa gggcgatggg ttaatcatat taaaaccaat
     6721 tatgaaatac cttttaaagc atatttgagt aaagcaacaa ccgtttttcc aataactaaa
     6781 ctttcgcccg taaagaactt tgatgggaga tggaaatcct cttttaaaga catgcatggt
     6841 gaagaaattg ccataggttt gtttcagcaa aatggggcga atgcaacagg tactttttta
     6901 acagaaacag gagattatcg ttttttatcg gggaatgtag attccaacac attgtatttg
     6961 tctggatttg atggaagcca tgcttattta tttgtcggca ctcaagacga tcaaggtaaa
     7021 attaaaggat tgtttttaag tggtacacac catcgttcgg tttggactgc cgaaagaaat
     7081 gatacttttc aattaagtga tgcagattca attacaactt ttgttggcga gtcaaaagat
     7141 gacattattc ttaatttcaa gaatttagac ggcactaatt tcgaatatcc gaacgagcag
     7201 tttaaagata aagttgttat cattcaaatt atgggtactt ggtgcgccaa ttgcatggat
     7261 gaaacggtgt atttcaaaga attgtatgat aaatacaatg caaaaggtct tgaaatcatt
     7321 acggttggtt atgaaacagg tgcaactttt gaagaatgtt ccaaacaatt gcaagccttt
     7381 cagcaacgtt tcaatttgaa aaacaccatt gttgtaggtg gaaatgtgcg caaaaacaaa
     7441 gtgatggatg attttacatt cttatctaat ttttcatctt atccaacgtc tatttttatc
     7501 gataaaaaag gaaatgttgc gcgcattcat accggttttt cgggtccatc aacaggaaaa
     7561 atttatacag attacaaaca agaaaccgaa cgtttaattg aagaattaat aaatctttaa
     7621 cagttgattt ggtagattca atttattttt actcaaaatt aagcaacatg aaaaaggcaa
     7681 ttatttggat tagcatttta ttttttcttc cttcatgtgg tgggaatgat acagaattta
     7741 aagacttgcg tggaaaagaa gtggaaagca ccattgctgc aacgcctctt tctcaaaaag
     7801 aattggatga gagcttagca aaaattcgtg cagaagaaga agccaaagaa gctaaagaac
     7861 gttctatgca aaccaccctt tcttttgata aaacagaaca cgattttgga aatgtaaaag
     7921 cacaaacacc caacgaagct tcgtttaaag taactaatac cggtgataag cctttaataa
     7981 ttagcaatgt tgaaacaagt tgcggatgta cgaattctaa aaaaccagaa aaacccattt
     8041 tacctggtca atcggatgaa atagcagtta cgtttaaatc caacccagga caaacaggtg
     8101 agcaacataa aaccatcacc gtttctgcaa atactaaaga aaaaacgcat caattgaaaa
     8161 tcagagcgtt tgtcaaataa tcaaatcatc ataacaataa atgatgttgt accctttttc
     8221 ccgaaaataa gcggttgctt gcgatttatt actgcatgtt gccataataa aatcgccacc
     8281 ccaagccccc aacgacttga tacagtgtgg ataatctgaa aacaaacgct gttgaatagg
     8341 ggcaatagct aaaattgtac ttaaaagttg ttcgcttgca ttcattaact tttcccattc
     8401 ttcaatagtg gtgcaaacag ttgcttgctc aacaattgca ttcattgcgt tgatgtgcgc
     8461 tgtagttgta attttatttt taaaattagc tatttcgttg gcactacttt gttttgctcc
     8521 taagtaaaca aatataagtt gattggaaat ggaaggtgat aattgaacgt tttgcaaggt
     8581 tttatcgcgc atggtgtaca caattggctg acgagacaag gcagttgcca aatcatagcc
     8641 cgaacctcca aaagaatgtt ctaataaata ataagcatca actcccgacc attgactaag
     8701 caaagaaagc agcgttgaac tggtgccaaa accaaacata cggttaaaat tgagactgaa
     8761 ttctagatta agtccagcac attttaacgt tggatttttt tcgcaaataa gttgtaataa
     8821 acgttgtgca atttttgctt tctcctcatc cgaagtttga atgatttcca acgatgaaga
     8881 gaaacgtata tcgagccaac aaacagcgtt ttcgtcgaaa caagtccaat gaatttcagg
     8941 gagttgttgc gatgaaaccg taagtgtttg ctgatatttt aatggaaacg acaaacattt
     9001 tgagcctctt aaaaccaaat attcaccaaa gaacaataat tttccggcgg cagagaactg
     9061 ttgtttttcc attgcaatga agcctttatt taactttttc ggctggaatt aaattccatt
     9121 tttcggctaa attacgaaca gcagaaacag aaaccacttt gtctttaaaa tgttcaacaa
     9181 tagcattcct gtgcacctct tcaatgttca actgtttgat gatgttcaat aagtgcattt
     9241 tcatgtgacc ttgttgaatg cccaccgtta ccaagttttt cactgctgag aagttatttg
     9301 ccataccaat cgttgcagca attgccatta attctttcgc attgggatta tcaagtagct
     9361 ctaaactaag ttttactagc ggatgtaacg aagttaaacc acccacaact cccaaagcaa
     9421 atggaatttc catttcaaaa cggaatagat ctccgttcaa ttccacatgg ctaagcgatg
     9481 tgtattttcc gtcttttgca gcgtatgtat gtgcgcaagc ttcaacagct ctaaaatcgt
     9541 ttcctgtagc caatactacc gaatcaacgc cgttcatgat tcctttgttg tgtgtagttg
     9601 cacggtgagg atctacttcg gcaacgcgta cacccaaacg aaatcttcga gcaaattctt
     9661 tcccgctgat gcccggtaca atgttgtcaa actcattgat aggtgtttca accgtacaac
     9721 gaacacggca gttaggcgtt aaattggata aaatacacat gattatttcc aacccatcc
//
LOCUS       DAOQVQ010000032         9636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7907_length_9636_cov_4.073061, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000032 DAOQVQ010000000
VERSION     DAOQVQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7907_length_9636_cov_4.073061"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..604
                     /locus_tag="PKN22_01905"
     CDS             68..604
                     /locus_tag="PKN22_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41486.1"
                     /translation="MKYLGIIALAFLLASCGAKVAYTDQIKEDFNLYTEAHMKKVQFY
                     ISQAIVLQRVKQTGGQGTTSDGALVTNSNREENRITIQAQTPGIFESYGKNNEMLIRF
                     EVGQGRYLSFKVRQANNSNAKYYLEATWDMNKGGELMYGNEKYTVESYAGNAYLMVML
                     KKLQKTKRQDRVVKGLKV"
     gene            630..2687
                     /gene="ppk1"
                     /locus_tag="PKN22_01910"
     CDS             630..2687
                     /gene="ppk1"
                     /locus_tag="PKN22_01910"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNS41487.1"
                     /translation="MVIFNRELSWLSFNERVLQEALDPRNPVIERMRFLGIYSNNMDE
                     FFRVRVANIRKMIEVGDKNIDGFKGNPKDLFKEIRLIVLRQQKQFEIAYQKILKELEQ
                     HNIYHIQEKDLTKEQVSELKLYFNLKLKHAIVPIMLNAATPFPRLKDSGIYLAIKMIY
                     LNKTKVRYALIKIPSTISRFYIIKEAEKTKVILLDDIIRLNLDEIFSIFIYDKAEAYT
                     FKFTRDAELNLDDDISTSLKDKVEQSIKLRKKGTPVRFVYDNEMPIDLLEYLLRHLGL
                     EYGVNTIAGGRYHNFKDFMSFPDFDNPNFVYKPLPPLNHPELENRRSLLKYILRKDVM
                     LHYPYQRFDYLVDMLREAAIDPKVVSIKINVYRVTRESPILKALMNAISNGKQVTVFL
                     ELMARFDEENNLYWSNRLKENGATVLHGRQDLKVHSKLFQIVRQNKKEKQYISHIGTG
                     NFHEKTAKIYGDLSFITADEKIGQEVEKVFDLLASESDSRDEVFEELLVSPFNTKKRI
                     VELIENEIAAAQAGIKAEIFIKLNNLTDQDLIELLYKASNAGVHIKMVIRGICCLVPG
                     VEGLSSNIKVYSIVDRFLEHARILKFYNQGNSLYYISSADWMERNLYKRIEVMAPIKD
                     AALQKEIDLIFEYQFKGTAKTRIIGKNQKNKYRKSDVNTFHAQMELYNHYKQLLDEKN
                     NAV"
     gene            2746..3447
                     /locus_tag="PKN22_01915"
     CDS             2746..3447
                     /locus_tag="PKN22_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41488.1"
                     /translation="MFKSFLKRLKYYGIGFGMGVIIVMFLLPNRSCSWTPSNRVKNMI
                     LGRVLTVSEIDWKLMQSKGITKKDILSVLDDGEVDFGKSKKDQQLKVYLIEKELKGKG
                     NFKFYFTLPDEGYISEVKIGEIDAKKVENTKTGYGIFLSVPHDEFLIATDSTQKINCQ
                     LDAIGVENTKQLYWAIRKTGRINFEKSNLNVQPKPEQYIEFVYGNDTIAAQSIWYKSK
                     IFISNFIADKAKSCN"
     gene            3536..4579
                     /gene="lpxD"
                     /locus_tag="PKN22_01920"
     CDS             3536..4579
                     /gene="lpxD"
                     /locus_tag="PKN22_01920"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNS41489.1"
                     /translation="MEFSAEQIAGLLQGEIVGDANVIVNGLAKIEEGSKGKLSFLSNP
                     KYEEYIYKTESSICIVNKTFEPTKELPSTLTLVKVENAYACFAKLLDVYDQMKRKQPK
                     IEHNSYISDKATVGKDLYLGANAYISDGAVIGDNVVIYPNSFIGDNVKVGDNTTIYPN
                     VTIYADCQVGKNCVIHAGAVIGADGFGFSPDENGVFSKVPQIGIVIIEDNVDVGANAT
                     IDRATMGATIIRKGAKIDNLCQVAHNVELGENSALAAQVGIAGSAKIGKRVLIGGQAG
                     ISGHLHIADGTMIVAQSGVPNTIKKPGAYMGAPAIPIEDFKKSYFGFRKLPFILNKIQ
                     ELDTKLKQTTKED"
     gene            4581..5978
                     /locus_tag="PKN22_01925"
     CDS             4581..5978
                     /locus_tag="PKN22_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNS41490.1"
                     /translation="MSEKQRTLKQSVKLAGIGLHTGQLVTVEICPAPENHGYKFQRVD
                     LEGEPIIKADADLVVATDRGTTLEANGARVYTTEHLLAALYGCQVDNALIKLDAPEVP
                     IMDGSSHLFVTAIQEAGFEEQTAERDYFVLTENIKWEDAEKGIEFLAVPDSEYRLTVM
                     VDYNSPVLGTQHATIYNLSEFVPNISKCRTFVFLRELEFLAKNNLIKGGDLDNAIVLV
                     ERENISKAELDSLAKLLGKENLDIKVEGIGVLNSTKLQYENEPARHKLLDIVGDLALV
                     GRPIKAHILAARPGHSGNVRFAKVLKEQIKKQEKGGKQFDLTKTVFDINQIESMLPHR
                     FPFLMVDRIIEITDNSIAGIKNITMNEPIFTGHFPGNPVFPGVLQIEAMAQVGGIFAL
                     NQVEDPHLYSTYFMKIDNVKFKQKVIPGDTVVFELTLISPIRRGLVNMAGKGYVNGQL
                     VCEAEMLAQVIKDKV"
     gene            5982..6761
                     /gene="lpxA"
                     /locus_tag="PKN22_01930"
     CDS             5982..6761
                     /gene="lpxA"
                     /locus_tag="PKN22_01930"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNS41491.1"
                     /translation="MISNLAQVSEKAQLGQNVQIDAFSVIHENVVIGDNTIIHSNVVI
                     FPGARIGKNCEIFPGAVISAVPQDLKFEGEETTVEIGDNTKIRECVTVHRATKDKWVT
                     KIGSNCLIMAYVHIAHDCQIGNNVILASYVGLSGHCIVDDYAILEGSVGTQQFMKIGK
                     HAFLAGATLVRKDVPPYVKAAREPITFAGVNSIGLRRRGFSDEAVREIEDAYRIIFVL
                     NNNVSKGVQAVKEQMPDSVYAKEIIEFIETSDKGIIRGMIG"
     gene            6778..8217
                     /gene="rlmD"
                     /locus_tag="PKN22_01935"
                     /pseudo
     CDS             6778..8217
                     /gene="rlmD"
                     /locus_tag="PKN22_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
     gene            8272..9513
                     /locus_tag="PKN22_01940"
     CDS             8272..9513
                     /locus_tag="PKN22_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNS41492.1"
                     /translation="MLVEFISIGDELLIGQTVNTNASWLGEKFSTLGASIHRIVTIRD
                     TESAIHLAIDEALKTADVVIITGGLGPTKDDVTKKVITNYFNDELVIDTDTLERVKQI
                     FSRTNRPMLDVNIQQAAVPSKCTVLTNRQGTAPGMWLEKDGKIVISLPGVPFEMKGIM
                     EQEVFPRLQARFSDLKFFSITANIQGIGESYLADKMSDWENRLRAAGFELAYLPSPGI
                     IRLRITSRNGVQDEQQILAYFKEVEAAYPDNFFGYGDISLAEVVGNLLKEGNYTLSTA
                     ESCTGGAIGAAIVSVPGSSAYFQGGFLTYSNELKHQELGVSQQLFETVGAVSEEVVIA
                     MAKGCQQRLKTDFAIAVSGIAGPDGGTDEKPVGTVWVAIATPNEIVTKKLLLSDNRER
                     IINRTVLTALNLLRTTLMNLK"
BASE COUNT         3268 a         1593 c         1951 g         2824 t
ORIGIN      
        1 tgcaactgac aattaaggaa tggtttttga atcagacaaa atcaatataa ttgtaaaaaa
       61 atcaatcatg aaataccttg gaattattgc actcgctttt ttgcttgcat cgtgcggagc
      121 taaagtagcc tataccgatc aaattaaaga agattttaat ttgtataccg aagcacacat
      181 gaaaaaggtt cagttttata tttctcaagc cattgttttg caacgtgtta aacaaacagg
      241 tgggcaagga acaacttctg atggggcgtt ggtaacaaat agcaatagag aagaaaatag
      301 aattacaatt caagcacaaa cacctggaat ttttgaatcg tatggcaaaa acaatgaaat
      361 gctaatccgt tttgaagttg gacaaggtag gtatttatcg tttaaagtca gacaggcaaa
      421 caacagcaat gctaaatatt atttagaggc tacttgggac atgaataaag gtggagagct
      481 gatgtatgga aatgaaaaat acaccgtaga atcctatgca ggaaatgcct atttgatggt
      541 gatgctcaaa aaattacaaa aaaccaaacg ccaggatcgt gtggtgaaag ggttaaaagt
      601 ttaaaatagc attccttttt atcacatcta tggttatttt taacagggag ttaagctggt
      661 tgtcctttaa tgagcgggta ttgcaagaag cattagaccc ccgcaatcct gttattgaac
      721 gcatgcgatt tttaggaatt tattccaaca atatggatga atttttccgt gtgcgtgttg
      781 ccaatatccg taaaatgatt gaagttggtg ataaaaatat tgacggtttt aaaggaaatc
      841 ccaaagacct gtttaaagaa atccgattga ttgttctgcg ccaacaaaaa caatttgaaa
      901 ttgcttacca gaaaatttta aaagaattag aacaacacaa catttaccac atccaagaaa
      961 aggacttaac aaaagagcaa gttagtgaac taaaattgta tttcaacctc aaattgaaac
     1021 atgctattgt tccaataatg ctcaatgccg ccacaccttt tccccgttta aaagattcgg
     1081 gcatttattt ggctatcaaa atgatttacc tgaataaaac gaaggtgcgc tatgcgttga
     1141 ttaaaattcc ttctaccatt tctaggttct atatcatcaa agaagctgaa aaaacaaaag
     1201 tgattttgct cgatgacatc attcgcttga atttggatga aatattctct attttcatct
     1261 acgataaagc agaagcatat acatttaaat ttacacgaga tgctgaatta aacctagatg
     1321 acgatatttc aacttcactc aaagacaaag tggaacaaag catcaaattg cgcaaaaaag
     1381 gtacgcccgt aaggtttgtg tacgacaatg aaatgcccat cgatttgttg gaatatttgt
     1441 taagacacct agggttggaa tatggcgtaa atacaattgc aggtggacgt tatcacaatt
     1501 ttaaagattt catgtctttt ccagattttg ataaccctaa ttttgtttac aaaccattgc
     1561 cgccactgaa tcatccagaa ttagaaaata gaaggagctt actcaaatac atccttcgca
     1621 aagacgtaat gctgcattat ccctaccaac gatttgatta tctggtggat atgttgcgcg
     1681 aagcagccat tgaccccaaa gttgtttcaa taaaaatcaa cgtgtaccga gtaacgcgtg
     1741 agtcgcctat tttaaaagcg cttatgaacg ccatcagcaa cggcaaacaa gtaacggttt
     1801 tcttagaatt aatggcgcgc tttgatgaag aaaataattt gtattggtcg aaccgattaa
     1861 aggaaaacgg agcaacagtt ttgcacggtc gtcaagactt gaaagtgcat tctaaattgt
     1921 ttcaaattgt tcgtcaaaat aaaaaagaaa agcaatacat ttcgcacatc ggaacgggta
     1981 attttcatga aaaaaccgca aaaatttacg gcgatttaag tttcattaca gccgatgaaa
     2041 aaattggcca agaagttgaa aaagtatttg atttattagc ttctgagtcc gattctagag
     2101 atgaggtttt tgaagaatta ttggtctcgc catttaatac aaagaaaaga attgttgaac
     2161 tcatagaaaa cgaaattgct gctgcccaag caggtataaa agcagaaatt tttatcaagt
     2221 taaacaactt aaccgaccaa gatttgattg aactgctata caaagcaagc aatgcaggtg
     2281 tgcacattaa aatggttata cgaggaattt gttgtttggt gccaggtgta gaaggacttt
     2341 cgtccaacat taaagtttat tccattgttg atcgcttttt ggaacacgcg cgtatattaa
     2401 aattttacaa tcaaggtaat tcgctttact acattagtag tgctgattgg atggaaagaa
     2461 acctttacaa gcgtatcgaa gtaatggcgc caatcaaaga tgctgcacta caaaaagaaa
     2521 ttgatttgat ttttgaatac caattcaaag gcactgccaa aacccgaatt attggaaaaa
     2581 atcaaaaaaa taagtaccga aaatcagatg taaatacctt tcatgcacaa atggaattgt
     2641 ataaccacta caaacagttg ttggatgaaa aaaataatgc ggtgtaattg ttcgatttaa
     2701 cactgccttt caagttaaat atgtaaattt gcacaaattt tttacatgtt taaaagtttc
     2761 cttaagcgct taaagtatta cggtattggt tttggaatgg gagtcatcat tgtgatgttt
     2821 cttttgccca acagaagttg ttcgtggaca ccctccaacc gtgtgaaaaa catgatttta
     2881 ggtcgtgttt taaccgttag tgaaattgat tggaaattga tgcaatccaa aggaattact
     2941 aaaaaagata tcttgtctgt tttagacgat ggagaagtgg actttgggaa aagtaagaaa
     3001 gaccaacaac tcaaggtgta tttaatcgaa aaagaattga aaggaaaagg aaatttcaaa
     3061 ttctatttca ctttgccgga tgaagggtat atttctgaag tgaaaattgg tgagatagat
     3121 gccaaaaaag tagaaaatac caaaacaggt tacggtattt ttctttctgt tccacacgat
     3181 gaatttttaa ttgccaccga ttctacgcaa aaaattaatt gtcagttgga tgcaatcggt
     3241 gttgaaaaca ccaaacagtt gtattgggca ataagaaaaa cagggagaat aaattttgaa
     3301 aaatcaaatt taaacgttca gccaaaaccc gaacaataca ttgaatttgt gtatggaaat
     3361 gacactattg ctgcgcaatc tatttggtat aaaagcaaga tttttatctc taatttcata
     3421 gctgataaag ccaaatcatg caattaatca gacaataagt tggtttaaat ggagattttt
     3481 cgcaaattct taaccaaaac acagtaaaac agatagattt ttatattttt gaaacatgga
     3541 attttcagca gagcaaatag caggattgtt acaaggagaa attgttggcg atgcgaatgt
     3601 aattgtcaac ggtttagcaa aaattgagga aggaagcaaa ggaaaacttt catttctttc
     3661 caatccaaaa tacgaagagt atatatacaa aacagaatcg agtatttgca ttgttaacaa
     3721 aacctttgaa ccaacaaaag aattgccttc tacacttaca ttggtaaaag tagaaaatgc
     3781 ctatgcttgc tttgcaaaat tattggatgt ttatgaccaa atgaagcgta aacaaccaaa
     3841 aattgaacac aattcctaca tttctgataa agctacagtt ggtaaagacc tttatttggg
     3901 tgccaatgct tatatttctg atggagctgt aattggtgac aatgttgtaa tttatcccaa
     3961 cagttttatt ggcgacaatg taaaagtagg cgataacaca actatttatc ccaacgttac
     4021 catttatgca gattgccaag tgggtaaaaa ctgtgtcatt catgccggtg ctgttattgg
     4081 tgccgatgga tttggtttta gccctgatga aaacggtgtg tttagcaaag ttccacaaat
     4141 tggtattgtt atcattgaag acaatgtgga tgtaggtgcc aacgcaacaa tcgacagagc
     4201 aacaatggga gcaaccatca ttcgcaaagg agcaaaaatt gataacttgt gtcaagttgc
     4261 ccacaacgtg gaattgggag aaaactctgc tcttgctgca caagtgggga ttgcaggttc
     4321 tgccaaaatt ggtaaacgtg tattgattgg tggtcaagcc ggaatttctg gtcacttgca
     4381 cattgccgac ggaacaatga ttgttgcgca atcgggtgtt ccaaatacca ttaaaaaacc
     4441 tggtgcttac atgggagctc cggccattcc aattgaagat ttcaaaaaat cgtattttgg
     4501 atttagaaaa ttgccgttta tcttgaataa aattcaagaa ttggatacaa aattaaaaca
     4561 aacaacgaaa gaagattaac atgtctgaaa aacaaagaac gcttaaacaa agtgtaaaat
     4621 tagctggtat tggtttgcat acgggtcaat tggtaactgt agaaatttgt ccagcccccg
     4681 aaaatcatgg ctataaattc caacgagttg acttggaagg tgaacccatc atcaaagcag
     4741 atgctgattt agttgttgca accgatagag gaacaacttt ggaagcaaat ggtgcacgag
     4801 tttatacaac agaacacttg ttggctgctt tgtatggttg ccaagtggat aacgcattga
     4861 ttaagttgga tgcgcccgaa gtgccaatta tggatggatc ttcgcacttg tttgttacag
     4921 caattcaaga agcaggtttt gaagagcaaa cggcagagag agattatttt gtattgacgg
     4981 aaaacatcaa atgggaagat gcagaaaaag gaattgaatt tttagctgtt cctgattcgg
     5041 aataccgttt gactgtgatg gtggactata attcaccggt tttgggaact caacatgcaa
     5101 cgatttacaa cttgtcggag tttgtgccaa atatttcaaa gtgtagaacc tttgtattct
     5161 tgcgcgagtt agaatttttg gctaaaaaca acttgattaa aggtggagat ttggacaatg
     5221 ccatcgtttt ggtagaacgc gaaaatatct ctaaagcaga attggatagc ttggcaaaat
     5281 tgcttggcaa agaaaattta gacatcaaag tagaaggaat tggtgtactc aacagcacta
     5341 aattacaata cgaaaacgag cctgcacgac acaagttgtt ggatattgtg ggcgacttag
     5401 cattggttgg gcgcccaatt aaagcgcata ttttggcagc tcgaccagga cactctggaa
     5461 acgtacgttt tgcgaaagtg ttgaaagagc aaatcaaaaa acaagaaaaa ggtggaaaac
     5521 aatttgattt aacaaaaaca gtttttgata tcaaccaaat tgaatccatg ttgccgcacc
     5581 gtttcccatt cttaatggtg gatagaatta ttgaaattac cgataattca attgcgggaa
     5641 ttaaaaatat caccatgaac gaaccgattt ttacggggca tttcccagga aacccagtgt
     5701 ttccaggtgt attgcaaatt gaagcaatgg cacaagtagg tggtattttt gccttgaatc
     5761 aagtagaaga tcctcatttg tattctacct atttcatgaa aattgataat gtgaaattca
     5821 aacaaaaagt tatccctgga gatacggttg tttttgaatt aacgttgatt tcaccaatta
     5881 gaagaggatt ggtaaacatg gctggaaaag gttatgtaaa cggacaatta gtttgtgaag
     5941 cagaaatgtt ggcacaagtc atcaaagata aagtttaaga aatgataagt aatcttgctc
     6001 aagtttctga aaaagcacaa ttgggtcaaa atgttcaaat tgacgctttt tctgttatcc
     6061 atgaaaatgt tgttatcgga gataatacca tcattcattc aaatgttgta atctttcccg
     6121 gagcaagaat tggaaaaaat tgcgaaattt tccctggtgc cgttattagc gctgtgcctc
     6181 aagatttaaa atttgaaggc gaagaaacaa ccgttgaaat tggtgataac actaaaattc
     6241 gcgagtgcgt aaccgtgcat cgtgcaacaa aagataaatg ggttacaaaa attggtagta
     6301 actgccttat tatggcctat gtgcacattg cccacgattg tcaaattggt aacaatgtga
     6361 ttttggcatc ctatgttggt ttgtctggtc attgcattgt tgatgattat gcaattttgg
     6421 aaggtagtgt tggtacacag caatttatga aaattggtaa acacgctttc ttggcgggag
     6481 caacgttggt gcgtaaagat gtgcctcctt atgtgaaagc agctcgtgaa cctattactt
     6541 ttgccggcgt caattccatt ggcttacgaa gaagaggttt ttctgacgaa gcagtgcgtg
     6601 aaattgaaga tgcttatcgt attatttttg tgttaaacaa caacgtttca aaaggtgtgc
     6661 aagcggtaaa agaacaaatg cccgattctg tttacgccaa agaaatcata gaatttattg
     6721 aaacttcgga taagggaatt atcagaggaa tgattggtta atttaattga taacaaattg
     6781 aaagtttatc atagaggaga ggtgataacg gtaaccattt ctggttatgc gtttggtgga
     6841 aatggtattg caaaaattcc aaccgaaaat ggcgattttg tagtgtttgt tggaaatacg
     6901 tttccgggac aaaccgtgca agcaaaagtt gaaaaaatca aaaaacgcta ttgcgaagca
     6961 aaattaattc aagtaattga acgttcgccg cttgaaaaag tttcagcatt tcaagaaatt
     7021 tctggcgccc cttatatcta tgttccagtt gaagaacaag aaaaagaaaa attacaaagc
     7081 accctcgaag tatatcgtaa attgggtggt tttcaagata ttaaagaacg ttttgattcc
     7141 tttatttctt cccccgttca ttttttctac cgcaataaaa tggaatacag cttttcgtgc
     7201 attgaacacg atatgcaaac cggagaagat attgatgatg cttttgctgt aggttttaaa
     7261 cgtagaggaa cttggtggaa agtagaaaat ttagccaaac caagcggaat gtttgatgag
     7321 gaatgggaaa caaaactcat tcaaatacgc gattttctta aaaacacgaa tttacccgca
     7381 tagcatccac cgaagaaaac tggtttcttc cgccatatta ttgtcagaaa atcgtttgac
     7441 caagaccaac tattggtgaa cttggtaaca tcttctgaag ggttaaaaca ctttgatgta
     7501 aaagcatttg gcgcattttt aaaagaactt tttggaagta gaattgctgg attccaacat
     7561 acgataaacg atgatgtagc cgatcgctct aaaatagaaa atggtaattg tcaacttgtt
     7621 tttggtcaac ccattgtgat tgaaaaatta ctaggacttt cttttgaaat ttcgatggaa
     7681 agtttctttc aaaccaatcc aaaaagtgcc gaattgttat acaccaaagc attggactat
     7741 gtttttgaag aagaaattcc tcaaaacgat gtggtgatgg atttgttttg tggaacagga
     7801 accataggtc agattttggc aactcgcaga acggacgtgc gcatcattgg tgtggacatt
     7861 gttgaagaag ccattgccga tgctaaacgc aatgctcaac acaacggatt ggaaaatgca
     7921 gtagaatttt atgctgccga tgttggaaaa ttcttgcgtg agcatccaga atttacagga
     7981 cacatttcaa gcatcatcct cgatcctcca cgtgccggaa ttgcaccaaa aacgttgcaa
     8041 aaagtcattg atttaggagc caaaaacatt gtgtatgttt cgtgtaatcc gagcacccaa
     8101 gcacgtgatg ccgatacgtt gcgtaaagct ggatataact tggaaaaatt aacgttggtt
     8161 gatcaatttc cacataccgg acacattgaa gccattgcaa aatttaaaaa gggttaaccc
     8221 actttcccac tttcttttta ctacctttga atggtctaaa aatcattcaa tatgcttgta
     8281 gaatttattt ccattggtga tgaattgctc attggtcaaa ctgtaaacac caacgcttct
     8341 tggttgggag aaaaattttc aactcttgga gcatccattc accgtattgt tactattcga
     8401 gatacagaat cggcaattca tttggcaatt gatgaggctt tgaaaactgc cgatgtggtg
     8461 ataattacag gtggtttagg gcctaccaaa gacgatgtta ccaaaaaagt tattaccaac
     8521 tatttcaacg atgaattggt gattgataca gatacattgg aacgagtaaa acaaattttt
     8581 tcacgcacca atagaccaat gttagatgtg aacattcaac aagcagctgt tcccagtaaa
     8641 tgcaccgttt taaccaatcg tcaaggaact gcgccaggca tgtggttgga aaaagatggt
     8701 aaaattgtta tttcacttcc cggggttcct tttgaaatga aaggaatcat ggaacaagag
     8761 gtttttcctc gattacaagc acgcttttca gatttaaaat tcttttctat cacagctaat
     8821 attcaaggta ttggcgaatc gtatttagcc gataaaatga gcgattggga aaaccgattg
     8881 cgcgcagcag gatttgagtt ggcctattta ccttcaccgg gtataatacg cttgcgtatt
     8941 acctctagaa atggagtgca agacgaacaa caaattttag cttatttcaa agaggtagaa
     9001 gctgcctatc ctgataattt ctttggttat ggcgatattt ctttagcaga agttgttggt
     9061 aacctgctta aagaaggtaa ttatacacta tcaacagctg aaagttgcac cggtggagct
     9121 attggagcag ccattgtatc cgttcctggc tcatccgcct attttcaagg aggtttttta
     9181 acgtattcca acgaattaaa gcaccaagaa ttgggcgttt ctcaacaatt atttgaaaca
     9241 gtaggagctg tgagtgagga agtagtcatt gccatggcaa aaggttgtca acaacgttta
     9301 aaaacagatt ttgcaattgc agtttcgggt attgcgggtc cagacggtgg aacagatgaa
     9361 aaacctgtgg gaacagtttg ggtggctatt gcaacaccca atgaaattgt aacaaagaaa
     9421 ctattgctat ccgataacag agagcgtatc attaacagaa cagtgctaac tgctttaaat
     9481 ttacttcgta caactttaat gaacttaaaa taatttactt cgattgagca gagtgatcaa
     9541 ccaccacgct ataaaataca aaagagagaa ttgaatgaat cctctctttt gtataaccta
     9601 aactctctct aatatgtatc tgttttggag tatttg
//
LOCUS       DAOQVQ010000033         9601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7950_length_9601_cov_4.149801, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000033 DAOQVQ010000000
VERSION     DAOQVQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7950_length_9601_cov_4.149801"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKN22_01945"
     CDS             complement(<1..562)
                     /locus_tag="PKN22_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS41493.1"
                     /translation="MKSKILQCAVELLNQQGINQTSFRDIASALNISDGHVRYYFKTK
                     ELLLTSIFDQLDNEMLYIISSSTFNDFQNLDEIITAKFAEGFTILVNYRFIMVESPKT
                     IHQFPKLASAYQNLIDNRKQLFLDFFEMLIQMGYFKSDFDKKQQEMVFNTLYIVADSW
                     IRYFIIKNNHKPAPSDIDFHSKLVFNI"
     gene            628..921
                     /locus_tag="PKN22_01950"
     CDS             628..921
                     /locus_tag="PKN22_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41494.1"
                     /translation="MAIERKCSNCNQWNKDEDYCVACGTVLSPQIIEEEREKEREIRR
                     ANVKITKVEEFFAKWKNNRFLPLKLLYYVIYSIWAIIMGVAFFFAYITVGVNG"
     gene            914..1303
                     /locus_tag="PKN22_01955"
     CDS             914..1303
                     /locus_tag="PKN22_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41495.1"
                     /translation="MDKLLKKPTDWLLLVAIFYFVIKISKQFVFIPIVHHYFTDLLFI
                     PTQLLICLIAVRFLKQNQSITIPFWLIVINVVLMSLLFEWYEPMVKNNTQQTADVFDV
                     VMYVLGGILYYLIQKTWFQRNIENRNN"
     gene            complement(1300..1605)
                     /locus_tag="PKN22_01960"
     CDS             complement(1300..1605)
                     /locus_tag="PKN22_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41496.1"
                     /translation="MNEIALKSVASLVGLSEISITSGNNEQLFEALSNYLNHLIEHDF
                     NQLVAILYRVDVSEEKVRQTLANAPKGSNSGKLIAQLILEREQQKMYWREKYKKGEI"
     gene            complement(1608..2858)
                     /locus_tag="PKN22_01965"
     CDS             complement(1608..2858)
                     /locus_tag="PKN22_01965"
                     /inference="COORDINATES: protein motif:HMM:NF040639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LETM1-related biofilm-associated protein"
                     /protein_id="HNS41497.1"
                     /translation="MLLPGSKGWINKYFSLVENKEIRLNYELVSAISPQEHLHYLSCK
                     TGIVFGYPICFVFFNKQDFNKWTDAEKLKVLLFETLLLVYIQKHGVSDTLKEDFTNKL
                     IEYYSAYNTKNIQDLLLFWVKEDKYAKIENILSKRVDVSTSIFDSRRWFDTMNNSLIY
                     LDVILFDRYLSCDDKTVLNTYSEYAYNVLIAIILAAKSDGEIDEKEKVIFNAFLNAAN
                     LEGEFKEKATQCLKSNVSFDYFTNLIHSNKLFSRYLMDVSLLTIYGNQEVEPTEIDSI
                     QHLLDVTGFEEDEVEESLATVEHFILNSDIDINFLSNKNTYEKAYDKITSRWTKILLR
                     NKDKLAKELSESKELIGLVKKSATQELTTEEKEKVKTQFFDVARSVPALAIFMLPGGA
                     ILLPLILNIIPDLIPSSFRDNEVQ"
     gene            complement(2891..3547)
                     /locus_tag="PKN22_01970"
     CDS             complement(2891..3547)
                     /locus_tag="PKN22_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNS41498.1"
                     /translation="MYKVFVENRPIIICEKNQTYFDCKTLFASNIKSIRDDLFPLFAD
                     QPIKEPIVLLSDDATADFKRLFADHKLIEAAGGIVRRKKRYLFIKRFGKWDIPKGKLD
                     KNEDPKLGAIREIEEECGINNPKIECLICHTYHTYLDTYKGKNKLVLKKTYWYELNYK
                     GPKDLFPQREEGITKAKWVKKSKLDKIRKNTYESILEVIDTYFKDNLIDMPEKKEDID
                     "
     gene            3555..4196
                     /gene="pyrE"
                     /locus_tag="PKN22_01975"
     CDS             3555..4196
                     /gene="pyrE"
                     /locus_tag="PKN22_01975"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNS41499.1"
                     /translation="MILNNDRAVKVAEFLLQIKAVKLQPQKPFTWASGWKSPIYCDNR
                     VTLSYPNIRTFIRQNFSAAILEHFGKPDVIAGVATGGIAQGALVAEELGLPFIYVRSS
                     KKEHGMGNQIEGYFEEGQKVVVIEDLISTGGSSLKAVEALREANLQVKGLAAIFTYGF
                     DVADKNFQDAKCPFITLTNYDVLLKKALDDKYITKDDIDSLKEWKVNPSEWKQ"
     gene            4184..4612
                     /locus_tag="PKN22_01980"
     CDS             4184..4612
                     /locus_tag="PKN22_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41500.1"
                     /translation="METIINKNIAMIIESTKVDVNAQPEAIYNFLKDANNLIHLLPKD
                     NVSDFKATEAECSFKVQGGVTITLVQNGGEAPNKLFLKSGEKSPFPFNLTIHIDAVGD
                     KANGHIVFDGEVNMFLKMMVEKPLTNLFNYMSNKLQTHFA"
     gene            4813..5166
                     /locus_tag="PKN22_01985"
     CDS             4813..5166
                     /locus_tag="PKN22_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41501.1"
                     /translation="MNEKIQHIIQEIRSKKEVLTRSLQQQTTRVQQLEQEVEQLKAEK
                     TTSAQELARAIEEQGQFQLVKAQLENENAELKSLLEAAKKQVVEAAVVEDKTNNNAQI
                     DELVREIEYCIGQLK"
     gene            5172..5462
                     /locus_tag="PKN22_01990"
     CDS             5172..5462
                     /locus_tag="PKN22_01990"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNS41502.1"
                     /translation="MSDKLSIKVVVAGRSYPLSVRPDEQEKVIKAAEEINKSIKTLQD
                     NYAVKDMQDLLAMTALQLAIKTAPKVEKVETPADYSAIEKALEQLSSEFNEQ"
     gene            5549..7081
                     /gene="rny"
                     /locus_tag="PKN22_01995"
     CDS             5549..7081
                     /gene="rny"
                     /locus_tag="PKN22_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNS41503.1"
                     /translation="MYLIIGLLLGIIGGGAGAFVFLNTAQKTKRDQLLKEAESEGEAI
                     KKEKILQAKEKFLKLKEEHEDAIKQRERKMQSTEDRMRNQEKQLSQKIEEVNRKEKQA
                     EKELKEFEAKREALDARQHELDKIHQKQVAELSKISGLSPEDAKNGLIEALKDEARTA
                     AMAHIKEIAEEAKLNANKEAKKIVIQTIQRVATEQVVENAVTVFNIDSDEVKGRIIGR
                     EGRNIRAIEAATGVEIIVDDTPEAIILSCFDPVRREIARLALHQLVSDGRIHPARIEE
                     VVAKTKKQLEEEIAETGRRTCIDLGIHGLHPELTRMVGRMKYRSSYGQNLLQHSREVA
                     NMCAIMASELGLNVKLAKRAGLLHDIGKVPDEEPELPHAILGMKMAEKYGEKPEVLNA
                     IGAHHDEIEMTSLISPIVQVCDAISGARPGARREIIESYIKRIKDLEALALSYDGVSS
                     AYAIQAGRELRVMVESERVSDQMVDELSFNISHKIQTEMTYPGQVKVTVIREKRSVNY
                     AK"
     gene            7081..8049
                     /locus_tag="PKN22_02000"
     CDS             7081..8049
                     /locus_tag="PKN22_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS41504.1"
                     /translation="MDSLANKVILVTGGAGFIGSNLVASLLELGATVRVLDNLETGHF
                     KNIEPFLKHPNFTFIEGSIADFDTCLKATDGVDAISHQAALGSVPRSIAFPHKTHEAN
                     ATGFLNIIHAAKENNVKRFVFASSSSVYGDSKASPKAIGTEGKLLSPYAVTKALNEEY
                     ASVYHKLHGMQTVGLRYFNVFGPNQDPNGVYAAAIPKFIDKLIKGEELVINGDGEQTR
                     DFTFVKNAVMANILALTTTNEAAFGRPFNVACSRFQSLNQVVAVLKENLANRGLLSQN
                     ATIVNGPDRAGDIRDSLADITETTALLGYSNVIHFEEGIKALIDSI"
     gene            8062..9345
                     /locus_tag="PKN22_02005"
     CDS             8062..9345
                     /locus_tag="PKN22_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS41505.1"
                     /translation="MKAIKRLTSNNFVKSVAVLMTGTVLSQVISLLISPVLTRIYEPE
                     VMSDLNLYTRIVGFLAALATARYELSLPLPKKDEHSYLIYRLSLKIAIIILVALSVVS
                     VLYFAFTGFDFKLISFTAITLTSAFFLTFTNLGVNWAIRKKEFKRISLSKILVSGVSN
                     GFKWLFGVLGMGSNGLLLASLLGFVVASLTFIKEWFSIDKNHSSFRSTKKTRVLAGIY
                     REFPSVSLPHAMVDLGKDLLLAFFMIFYFSKDVFAWYSLSYSVLQLPISVIGLAIGQV
                     FFSRCAELASNGQSTLPLLKKTLLTLFLVSIGPFTILFFFGEPLFGFVFGKNWSNAGY
                     YSQIMTIWFVVQFLNSVVSTLPSVLHRQREFFYLGILNATIQITGFGLLPLIIGKGND
                     EFPTILWIVSIAQSVFFCYVLFSMLQFAKKGVKKK"
BASE COUNT         3152 a         1562 c         1785 g         3102 t
ORIGIN      
        1 gtatattgaa caccaattta ctgtggaaat caatatcgga tggtgcaggt ttgtggttgt
       61 ttttaataat aaaataacga atccacgaat cggcaacgat gtaaagcgta ttgaacacca
      121 tttcttgttg ttttttatca aaatcggatt tgaaataacc catttgaatc aacatttcaa
      181 agaaatctaa aaacaattgt tttctattgt caatcaagtt ttggtaggca cttgccaatt
      241 ttggaaattg gtgtattgtt ttgggcgatt ctaccatgat aaatcgataa ttgaccaaaa
      301 tggtaaaacc ttcggcaaat ttagcggtaa taatttcgtc taaattttga aaatcattaa
      361 acgtacttga agaaatgata tataacattt cattgtctaa ctgatcaaat atggaagtta
      421 gcaacaattc tttggtttta aaataatagc gaacgtgtcc atcgctaata tttaaagcac
      481 ttgcaatatc cctaaaactt gtttggttga ttccttgctg gttaagcaat tctacggcac
      541 attgaagaat tttacttttc attatacaaa tataaaaaaa gtttgtattt ttgtctagct
      601 catttgagct aatataaatt tttagttatg gctattgaaa gaaaatgtag caattgcaac
      661 caatggaaca aggatgaaga ctattgtgtt gcgtgtggaa cagtgctatc gccacaaatc
      721 attgaagaag agcgtgaaaa agaacgtgaa atacgcagag caaatgtcaa aataacaaag
      781 gttgaagaat tttttgcaaa gtggaaaaac aaccgttttt tacctttgaa attattgtac
      841 tacgtgattt attccatttg ggcaattata atgggtgttg ctttcttttt tgcctatatt
      901 acggtgggag tgaatggata aacttttgaa aaaacctact gattggttat tgttggtggc
      961 aattttttat tttgttatca aaatttcaaa gcaatttgta tttattccaa ttgttcacca
     1021 ttattttaca gacctattat tcattcctac gcaattatta atttgtttaa ttgctgttcg
     1081 ctttttaaaa caaaatcaat ctattacaat tccattctgg ttgattgtta tcaatgtggt
     1141 attaatgtcc ttattgtttg aatggtatga acctatggtt aaaaataata cccaacaaac
     1201 tgctgatgtt tttgatgttg tgatgtatgt tttaggaggg attttatact atttgataca
     1261 gaaaacttgg tttcaacgca acattgaaaa tcgtaacaac taaatttccc cttttttata
     1321 tttttcgcgc caatacattt tttgttgttc gcgttctaag attagttgag caattaattt
     1381 tccagaatta cttccctttg gtgcattggc caaagtttga cgcacttttt cttccgacac
     1441 atcaacacgg tagagaatag caaccaattg attgaaatcg tgttcaatta aatggttaag
     1501 ataattgctg agtgcttcaa acaattgttc attgttgcca gaagttattg atatttctga
     1561 taaaccaaca agactagcaa ctgattttaa agcaatttca ttcatactta ttgcacttcg
     1621 ttatctctga acgaagatgg aattaaatca gggatgatat taagtattaa aggcaaaagt
     1681 atggcaccac ctggcaacat aaaaatagcg agggctggaa cagaacgtgc tacatcaaaa
     1741 aattgggttt ttactttttc tttttcttcg gtagttaact cttgcgtagc cgattttttt
     1801 actaacccta tcaattcttt actttctgat aattcttttg ccaatttatc tttattgcgc
     1861 aataatattt tggtccaacg agaggtaatt ttatcatacg ccttttcata cgtattttta
     1921 ttactcagaa agttgatatc gatatcgctg ttgaggataa aatgttctac cgtagccaac
     1981 gattcttcaa cttcatcttc ttcaaaacca gtgacatcta ataaatgttg aatagaatct
     2041 atttctgttg gttccacctc ttgattgcca taaatggtta aaagtgaaac atccattaaa
     2101 tagcgactaa acaatttatt agaatgaatg agatttgtaa aataatcaaa cgaaacatta
     2161 ctttttaagc attgcgtagc cttttcttta aattcacctt ctaaattagc agcatttaaa
     2221 aaggcattga aaataacttt ttctttttcg tcgatttcac catccgattt agcagctaaa
     2281 attatcgcaa tcaacacatt gtaggcgtat tctgaatacg tgtttaaaac cgttttatcg
     2341 tcacaactta gatatctatc aaatagaata acgtcaagat aaatcaaact gttattcatt
     2401 gtatcaaacc acctacgact atcaaaaata ctagttgaaa catctaccct tttagataaa
     2461 atattttcaa tttttgcata tttatcttcc ttcacccaaa acaacaataa atcttgaatg
     2521 tttttggtat tataggcaga atagtattct attaatttat tggtgaaatc ttcttttaat
     2581 gtgtcgctaa caccgtgttt ttgaatataa accagcagca atgtttcaaa caaaagcact
     2641 tttagttttt ctgcatcggt ccatttatta aaatcttgct tattaaagaa aacaaaacaa
     2701 atagggtaac caaaaactat ccctgtttta caacttaaat aatgcaaatg ttcttgcgga
     2761 gaaatagctg ataccaattc ataattaagg cgtatttcct tgttttctac aagcgaaaaa
     2821 tatttattta tccaaccttt tgaacctggc aacagcataa gagcattaat ttaaagcaaa
     2881 attaactatt tcaatcaata tcttcctttt tttcaggcat atcgattaaa ttatctttaa
     2941 aataggtgtc aatcacttct aggatggatt cataagtatt ttttcgaatc ttgtccaatt
     3001 ttgatttttt aacccatttt gcttttgtaa tgccttcttc gcgttgagga aataaatctt
     3061 tgggtccttt atagttgagt tcataccaat atgttttttt caacaccagc ttattttttc
     3121 ctttgtaggt atccaagtaa gtgtgatagg tatgacagat taaacattca atctttgggt
     3181 tgttgatacc acattcttct tcaatttctc ggatggcacc taatttggga tcttcatttt
     3241 tatctaattt tcctttagga atatcccatt ttccaaaacg cttgataaac aaatagcgtt
     3301 tttttctacg cacaatgcca cctgctgcct caattagctt atgatcagca aataaacgtt
     3361 taaaatcagc cgttgcatca tcggaaagca aaacaatagg ctcttttata ggttggtcgg
     3421 caaacaatgg aaaaagatcg tcgcgaatgg attttatgtt tgaagcaaag agtgttttgc
     3481 aatcaaaata ggtttgattt ttttcgcaaa taattattgg tctattttca acaaaaactt
     3541 tgtacatttg caccatgatt ttaaataacg atagagctgt aaaagtagca gaatttttgt
     3601 tacaaatcaa agcggtaaaa ttacaaccac aaaaaccgtt cacttgggca tcgggttgga
     3661 agtcgccaat ttattgtgac aacagagtaa ctttgtctta tcccaacata cgcacattta
     3721 ttcgacagaa ttttagtgct gccattttgg agcattttgg taaaccggat gtgattgctg
     3781 gtgtggctac aggtggaatt gcacaaggag cgttagtagc agaggaattg ggcttaccgt
     3841 ttatttatgt acgtagttct aaaaaagaac acggcatggg caatcaaatc gaaggttatt
     3901 ttgaagaagg ccaaaaagtg gtggtaattg aagatttaat ttcaactgga ggaagtagtt
     3961 taaaagctgt tgaagcatta cgtgaagcta atttgcaagt aaaaggtttg gcggcaattt
     4021 ttacatacgg ttttgatgtg gcggataaaa atttccaaga tgctaaatgt ccgtttatca
     4081 ctttaaccaa ttacgatgtt ttattgaaaa aagcattgga cgacaaatac attacgaaag
     4141 acgacatcga ctctttgaaa gaatggaagg tgaacccatc agagtggaaa caataataaa
     4201 taagaacatt gctatgatta tagaaagtac aaaagttgac gtgaatgcac aaccagaggc
     4261 aatttacaat tttttaaaag acgccaacaa tttaattcat ttattaccaa aagataacgt
     4321 ttctgatttt aaagcaacag aagcagaatg ttcttttaaa gtgcaaggtg gagttaccat
     4381 tacattggta caaaacggag gtgaagcacc caataaatta tttttaaaat cgggcgaaaa
     4441 atcaccattt ccattcaatt taaccattca tattgatgct gttggcgata aagctaacgg
     4501 acacattgtc tttgatggtg aagtgaatat gtttttaaaa atgatggtag aaaaaccgtt
     4561 aactaatttg tttaattaca tgtcgaacaa attgcaaaca cattttgcat aaaccgtttg
     4621 ataataagaa tttgaaatga gagatttttt aaaagtctct catttttttt ataattcgaa
     4681 gaacgacatg attgcaaaaa aacgctactg tttactgtga tttttgtaac aaaatagatt
     4741 tttttttaag ctgagtacaa aatttacttg tttatacgtt ttacaatgtt aactttgtaa
     4801 tcgctggaat tcatgaacga gaaaattcaa catatcattc aagaaatacg ctctaagaaa
     4861 gaggtgttaa cacgttcgtt gcaacaacaa acaacgaggg ttcaacaatt agagcaagaa
     4921 gtggaacaat tgaaagcaga aaaaacaact tctgcacaag aacttgcgcg tgcaatagag
     4981 gagcaaggtc agtttcaatt ggtaaaagca caacttgaaa atgaaaatgc tgaattaaaa
     5041 tcgcttttgg aagcagcgaa aaagcaagta gtcgaggcgg cagttgttga agataaaacc
     5101 aacaacaatg ctcaaattga tgaactggtg agggaaattg aatactgcat tggacagtta
     5161 aaataaaatg catgagtgat aaattgtcga taaaagttgt agttgctggt cgttcgtacc
     5221 cgctttctgt tcgtccagac gagcaagaaa aggtaataaa agcggctgaa gagattaaca
     5281 aatcaatcaa aacattacaa gacaattacg cagttaaaga catgcaagat ttgctagcga
     5341 tgacggcttt gcaattagca ataaaaacag ctcccaaagt ggagaaggtg gaaacacctg
     5401 ctgattacag tgccattgaa aaggcattgg agcaactgtc gagcgagttt aacgagcaat
     5461 agcagctatt caataagtga ttccccactg gatttattgg tttttacagt aaatcggtgg
     5521 gtttttttat ttaaaaattt ttaaagaaat gtatttaatt attggattgt tattaggaat
     5581 tattggtggt ggtgctggag catttgtgtt tttaaacact gcccaaaaaa ccaaaagaga
     5641 tcaacttcta aaagaagcag aatcggaagg agaggcgatt aaaaaagaga aaattcttca
     5701 ggcaaaagaa aagttcttga aattaaaaga agaacacgaa gatgctatta agcaacgtga
     5761 gcgcaaaatg caatctacag aagacagaat gcgcaatcaa gaaaagcaat tgtctcaaaa
     5821 aattgaagaa gtaaatcgca aagaaaagca agcagaaaaa gagttaaaag agtttgaagc
     5881 aaaacgcgaa gccctcgatg ctcgtcagca cgaattggat aaaattcacc aaaaacaagt
     5941 ggctgaatta tcaaaaattt ctggattaag tccagaagat gctaaaaatg gattgattga
     6001 agcattgaaa gacgaggcac gcactgctgc aatggctcac atcaaagaaa ttgccgaaga
     6061 agcaaaattg aacgccaata aagaggcaaa gaaaattgtg attcaaacca ttcaacgtgt
     6121 tgcaacagag caagttgttg aaaatgcagt aacggtgttt aacattgatt ctgacgaggt
     6181 aaaaggacga attattggtc gtgaaggacg aaacatccgt gcaattgagg cggcaactgg
     6241 cgttgaaatc attgttgacg acaccccaga agcaattatt ctttcttgtt ttgaccctgt
     6301 tagacgtgaa attgcacgtt tagcattgca ccaattggtt tcggatgggc gtattcaccc
     6361 tgcacgtatt gaagaggtgg ttgccaaaac gaaaaaacag ttagaagaag aaattgctga
     6421 aacgggtcgc agaacttgta tcgacttagg tattcatggt ttacatcctg agttaacgcg
     6481 tatggtggga cgtatgaaat accgttcatc ttatggtcaa aacttattgc aacactcccg
     6541 agaggttgcc aacatgtgtg caattatggc atctgaacta ggattgaatg tcaaattagc
     6601 aaaacgtgct ggattgcttc acgatattgg taaagtgcca gatgaagagc cagaattacc
     6661 acatgccatc ttgggtatga aaatggcgga gaaatacggt gaaaaaccag aagtattgaa
     6721 tgcgattgga gctcaccacg atgaaattga aatgacatcg ttgatttctc caattgtaca
     6781 agtatgtgat gccatttctg gtgctcgccc aggtgctcgt agagaaatta ttgaatctta
     6841 catcaaacgt attaaagatt tagaagcatt ggcattgtct tatgatggtg tttctagtgc
     6901 ttatgctatc caagctggta gagaattgcg tgtgatggta gaaagtgagc gtgtttctga
     6961 ccaaatggtg gatgaattgt cgttcaacat ttcacacaaa attcaaacag aaatgacgta
     7021 tccaggtcaa gtgaaagtaa ctgtaattcg cgaaaaacgc tcggttaact acgcaaaata
     7081 atggatagtc tagcaaataa agtaatttta gtaacaggtg gagccggttt tatcggttcc
     7141 aaccttgttg cttcactttt agagctagga gcaaccgtta gagtattgga taatttagaa
     7201 actgggcatt tcaaaaacat tgaaccgttt ttaaagcatc ccaattttac atttattgaa
     7261 ggcagcattg ccgattttga cacgtgttta aaggcaactg atggagtaga tgctatttcg
     7321 catcaagcgg cattgggttc tgttccacgt tccattgcct ttccacacaa aacacacgaa
     7381 gccaacgcta caggattttt aaacattatt cacgcagcca aagaaaacaa cgttaagcga
     7441 tttgtatttg caagctcaag ttcggtttat ggcgattcca aagcatctcc aaaagccatt
     7501 ggtaccgaag gaaaactgtt gagtccttat gcagtaacca aagctttgaa cgaggaatat
     7561 gcaagtgttt atcacaaatt gcacggcatg caaacagttg gattgcgcta tttcaacgtt
     7621 tttggtccca atcaagaccc caacggagtt tacgctgcag ccattccaaa attcattgat
     7681 aaactaatca aaggagaaga attggtgatc aacggtgatg gtgaacaaac acgtgatttt
     7741 acgtttgtaa aaaatgcggt tatggcaaat attttggcat taacaactac caacgaagct
     7801 gcttttggtc gcccatttaa cgtggcttgt agccgtttcc aatcgttgaa tcaagtggta
     7861 gctgtattga aagaaaactt ggcaaataga ggtcttttat cgcagaatgc aactattgta
     7921 aacggaccag accgagctgg tgacattcgt gattcgcttg cagacataac tgaaacaact
     7981 gcgctgttgg gttactccaa cgttattcac tttgaggaag gaataaaagc gttgattgac
     8041 agcatataac ttatacttat tatgaaagcc attaagcgtt taactagtaa caactttgtt
     8101 aaatcggttg ctgttttaat gacaggaacg gtgctttcac aagttatttc gcttttaatt
     8161 tcgcctgttt taacccgtat ttatgaacct gaggtgatga gtgacctcaa cctctacacc
     8221 cgtattgttg gatttttagc cgctttagca acagcgcgtt atgaattgtc attgccactt
     8281 cctaaaaaag acgaacactc gtatttaatt taccgattat cacttaaaat tgctataatt
     8341 attctagtag cactaagtgt tgtatctgtt ctttactttg catttacagg ttttgacttc
     8401 aaactaataa gctttactgc tattactttg accagtgcgt ttttccttac attcaccaat
     8461 ctaggagtaa actgggcaat acgtaaaaaa gaattcaagc gcatttcgtt gtccaaaata
     8521 ttggtttctg gggtgagcaa tggttttaaa tggctatttg gagtgttggg catgggaagc
     8581 aatggattgt tgttagcttc gttgcttggt tttgttgttg cttcgttgac gtttatcaaa
     8641 gaatggtttt caatagacaa aaatcactca tcgtttcgtt ccactaaaaa aacacgtgta
     8701 ttagcaggta tttacaggga atttccttcg gtaagccttc cgcatgcaat ggtagattta
     8761 ggaaaagact tactactggc cttttttatg attttttact ttagcaaaga tgtttttgct
     8821 tggtatagtt tatcgtattc tgtattacaa ttacctattt cggtaatcgg attggcaatt
     8881 ggacaagttt tttttagtcg ctgtgccgaa ttagcaagta atggacaatc tacgcttccg
     8941 ctgttgaaaa aaacgttgtt aaccctattt ttagtttcca ttggtccgtt taccattttg
     9001 tttttctttg gcgagccttt gtttggattt gtatttggta aaaattggtc gaacgctggt
     9061 tattattctc aaattatgac catttggttt gtggttcaat ttttaaattc ggttgtttcc
     9121 accttgccaa gcgttttgca tcgtcaacgt gagtttttct atttaggaat acttaatgct
     9181 accattcaga ttacaggatt tggtttgttg cccttgatta taggtaaagg aaatgatgaa
     9241 ttcccaacca ttttatggat tgtttccatc gcacaaagtg tcttcttttg ttatgtattg
     9301 ttttccatgt tacaatttgc aaaaaaggga gtgaagaaga aatagaccca attgcccatc
     9361 ttaaaatcaa ctgctatttt ttggaataca cattttatag ttgcatataa attttacaat
     9421 attaactttg tgatgattga acattaaatt taaaaaactt catttttgag tgattccaac
     9481 catacaaaac caattcaaat ttcgaagttt ttttaaggaa tagacaaaaa agaatatgca
     9541 ttcagcagaa ttaacacttg atgagataaa atcaaaacca ttagcaatag tatttgtttt
     9601 a
//
LOCUS       DAOQVQ010000034         9596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_7953_length_9596_cov_3.856933, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000034 DAOQVQ010000000
VERSION     DAOQVQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7953_length_9596_cov_3.856933"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKN22_02010"
     CDS             <1..1299
                     /locus_tag="PKN22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNS41506.1"
                     /translation="EMAISLRVGPVVLYPSLFLGIVICLLGIRSFFKWNNHLLIISIF
                     IGIWLIQGFVQYLLQQKTDLALFDLKVLMYQVITTGALFTTYSQLGKEFFFKLLLNGL
                     RYFLLILVAFAVFEMTTGIHLQGATTDKLINLPVSYLHYAPLFKYDNPNNFIAFYLFV
                     LTWIFLLDKSWGKNVYLMLCFTLFGYFVSEYADSNLGKLSFVLLALFLVSTLLIKIKR
                     IELVFGIIGVVLIGIVLYKQPIFLGPKYENGQNYRINGLIVVKDSLQHLKVISKENLT
                     QAEQQSILNTLDSIEASYPYKSYNVRKSLIENGVFLIQKHPFLGVGPGQFYQLHLDKK
                     VPHDTGTVTSAHCFPIEVTAVYGFIGWIYLFFIVFYFIKIALSKALSYEYYLKFFIAG
                     ILLVLIWMMPANFIFLEIHRLTLPLLVITFISLKQKPAHD"
     gene            1292..2542
                     /locus_tag="PKN22_02015"
     CDS             1292..2542
                     /locus_tag="PKN22_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS41507.1"
                     /translation="MTSEKSYRFHFLTILSGNTLSQLIPFVIAPFLTRVFTTEDFGVN
                     ANWLSIVTLIGIISTGRLEMAIPLPKSNKEASRIFSSGLIFTIIFTLLSLLLIVFDDQ
                     IANWYKDPELKYYLQMAPIGVFSVGLLNIFNNWSLRHRKYRRISVGKVVQSIVNNAGA
                     LFLGYLSFGVWGLLIAWVVSQFLNAFVLIEKGDLSYLFRRKNFNTSNFKSIVSKYKDF
                     PLVNSLHAFTDIFATQFILFYLIAVYFSKEELGLFFLMHRFVRAPIVLISSSVSQLYY
                     VEASKAIQQNLSGLPMAFKTMKTASIFAIPFLIVLLFFAPSIFSWYLGSEWREAGVYA
                     QYLAPMFFLSFFVSPLSGTPLIFNRQKTSFIINLCGYVLTLGSIFLGYMLEYTFAETL
                     ILYAISFSLLYFVLLVWYLSLLRK"
     gene            2539..3450
                     /locus_tag="PKN22_02020"
     CDS             2539..3450
                     /locus_tag="PKN22_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41508.1"
                     /translation="MRVIITGLPLFSKRMADDLNEFTHSKQFIYLNTYYSKWDQIKFI
                     CLLPFVAGVVSMNGVTSKSGILDYVCLWRKKIWMQWMGTDLLLANERSKNKTINTKYI
                     EKACHWVDAPWMQQELADISIASKTIDFKFLSNIPTTLKKYKKLEVCTYIPQNRQEFY
                     GMHYLCELAKAHPEIDFHVYGTKNPDDFVPKNVICWDWQPADVLHKKMSESAVFLRLT
                     KHDGYSVSVIEAMSLGCEVVSFFPFDKAHVVTETSILPTFARVIQEINERGLTPNFHL
                     QEYYQNKYKKTNVLTNLYNELKDYFEK"
     gene            3512..4651
                     /locus_tag="PKN22_02025"
     CDS             3512..4651
                     /locus_tag="PKN22_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41509.1"
                     /translation="MLAFATILANDFCVTVFALDEKTHTVKEHDNLYVRYSKSKSIFD
                     YLENKTTDSRSIHFLKTGMKVILAKVVKNPLKKWQKRTTKDLIQTHQQSPFDLIISSF
                     APQETHLAVIGFKKRYSEVPWIADMRDEMSKNPDISSKTRNALIQVEQYVNSYANAIT
                     TVSSPILNDFKTICPKVTHFKEIRNGYNHELTPLTHQNKVFTFGYFGSFYGGRKPVIF
                     FKALQHILDKNKDFEFKFVIVGAHKNFEIPAQVEKFVEIRPIQPYLEAVKMMMQMDMN
                     VIIQPRSQRKGVFSGKLFDYISAARPVLAMVDKDDVAAKMILDFDAGYVAECDDLDEN
                     ICAIEKAFDDWKSGIVKKASEENRLSLHRKNQVNELKLLINTILS"
     gene            4648..5676
                     /locus_tag="PKN22_02030"
     CDS             4648..5676
                     /locus_tag="PKN22_02030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS41510.1"
                     /translation="MKVAHIGSNSVHVSRFIEALNTHAVEQVLIAETPVDINFNIQTL
                     LVDFHTFNPFTLLRNKRLATNFLKEFNPDIIHIHQVNRIAFWIALIAKKLNIPVVTTA
                     WGSDVLLMPQKNWLYRYLVTKTLQNSKLVTADADVMLHAMQNLVPAGVRYEKIQYGID
                     PVELTLNKENIVYSNRLHKSLYRVDKIIEYFAEASAHDSNYQLIIAATGDESEKLKNL
                     VVDLNIENKVEFVGWVNAEQNRYWYSKAQFYVSIPSSDGTSVSLLEAISAGCIPIVSD
                     LPANLEWVKDGNTGIIEQPNENPILKAFQSQFDNALIENAQHVNKLASRSFTVPQFIT
                     FYHECISC"
     gene            5657..6946
                     /locus_tag="PKN22_02035"
     CDS             5657..6946
                     /locus_tag="PKN22_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNS41511.1"
                     /translation="MNASAVESNKYAVWYPRIIGLIAFFALFDLKANAILKLNVLPIL
                     ILGFLALVITGIVKKQLKFKLKPLAVLLIAFYVLYAIYAIYTRNPHQAGVYLENKLSF
                     LILPLLFSFRPIFTIKKDAIIYGWIGGTLALFLASIVNGVACYLNSEANSSCFISSNF
                     SFQYHPTYASVYYTVSFFLAYYAWKNKVKWFSGWATIAYMTVFFIAVFLCVSLAGMLF
                     FMLALSVAVLLFIKNKWGKKWMIISMIVLPFMLYGIIKGVPRSEGEWSNAVWYAEQYM
                     KEPRTYVEKCKPPHSGTQTRIIMWTVAYHACMHYPLGVGTGNVDEVLATYLRKYNQEN
                     MVEYQYNPHNQFLQTWLEIGVLGLLCLIGIIVYLMYIGIQQKNYLIILVALSLAFNCL
                     FESMLQRQSGIFFYPFLFFILEWFSTKINPTDNRLSE"
     gene            6962..8017
                     /gene="wecB"
                     /locus_tag="PKN22_02040"
     CDS             6962..8017
                     /gene="wecB"
                     /locus_tag="PKN22_02040"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNS41512.1"
                     /translation="MKKIITILGARPQFIKAAAVSAVFKNQVNELLVHTGQHYDANMS
                     DVFFEELNIPKPQYHLNIGSGSHGEQTGEMLKAIEKVILAEQPDFVMVYGDTNSTLAG
                     ALAASKLLIPVIHIEAGLRSFNKAMPEEQNRILTDHVSSLLFVPTQTAVDNLTNEGIT
                     NGVHLVGDVMYDGILHFSKIAEEKSDILEKYQLENNQFVLCTIHRAENTNDPARLKAI
                     FEGLNQAKVKTVLPLHPRTQKYIADYGITLENHIQVIEPVGYLDMVRLESACSKIVTD
                     SGGVQKEAFFLGKPCITMRDETEWVETIENGWNILVGADSSKIVNALQTFNPTVERKN
                     YFGDGTASQQILAHILK"
     gene            8029..9051
                     /locus_tag="PKN22_02045"
     CDS             8029..9051
                     /locus_tag="PKN22_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS41513.1"
                     /translation="MNQLSVVIPCRNEAPFITECIEAIYAAQLPSDTELSVFVVDGMS
                     TDATREIVLELTNRYPSLALIDNTKQLTPFAFNLGIHAKPCDYVQIIGARHIISENYL
                     INNLQTLQKDSSIWCVGGKIINEYVNPTGKVISLAMATQLGMGIGNFRTLKNSGFTDT
                     VTSPMYPYWVFEKIGFFDEELIRNQDDDFNYRVTKAGGKIYFDASISLKYYVRGDYKN
                     LWKQFFQYGYWKVFVNKKHKAVTTFRQLIPPLFTFYLLLNALSWLIGGYFAFIGTLPL
                     FLYLGLVTYVSYDIQQSNKELKLWDIFKTFPILHVGYGLGYLNGIWDFIILKKSPSEK
                     QKMLSR"
     gene            9093..9509
                     /locus_tag="PKN22_02050"
     CDS             9093..9509
                     /locus_tag="PKN22_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41514.1"
                     /translation="MKKINLIVFAALLLVFSACQKYEEGPAFSLLTAKTRLVNHWTIE
                     TYDIDGVNQPINVNTNLEMDFFKDNTFKRTWTIFGFMTAEEGDWEFTNNKLNVLLKKK
                     DGNMELYKIVKLTNNELKAKYTDQNGKTHSYYFKGK"
BASE COUNT         3142 a         1540 c         1668 g         3246 t
ORIGIN      
        1 gaaatggcga tatcattaag agttggtccg gttgtacttt atccaagctt atttttagga
       61 atcgttattt gcttgttggg tattcggtcc ttttttaagt ggaacaacca tttgctaatt
      121 atttccattt tcattggaat atggttaata caaggattcg ttcaatattt attacaacag
      181 aaaacagatt tagctttgtt tgatctgaaa gttttaatgt atcaagttat tactactggg
      241 gcattattta ctacctattc acaattggga aaagagtttt tctttaaact attactaaat
      301 ggattgcgct actttctatt gattttagtt gcatttgcag tttttgaaat gacaacaggc
      361 atccatttac aaggcgcaac aactgataaa ttaattaatt taccagtttc atatcttcat
      421 tatgctcctt tatttaaata tgataaccct aataatttta tagcttttta tttatttgtc
      481 cttacatgga tatttttgct ggataaatca tggggtaaaa acgtttattt gatgttgtgc
      541 tttaccctat ttggctattt tgtttcagaa tatgccgact ccaatcttgg caaactttct
      601 tttgttttat tagccttgtt tttagttagc acactattaa taaaaattaa acgcattgaa
      661 ttagtgtttg gtattattgg tgttgtgcta atcggaatcg ttttatataa acaaccaatt
      721 tttcttgggc caaaatatga aaatggacaa aactacagaa ttaatgggtt aattgttgtt
      781 aaagacagcc ttcaacattt aaaagttatt tcaaaggaaa atctaaccca agcagaacaa
      841 caaagtattt taaacactct tgactcaatt gaagcgtctt atccatacaa atcatacaat
      901 gttagaaaat ctttaatcga gaatggagtg tttctaattc aaaaacatcc atttttgggg
      961 gttggcccag ggcaatttta tcaattacat ttggataaaa aagttcctca cgatacaggc
     1021 acagttactt cagcacattg ttttccaata gaagttacag ctgtttatgg cttcattggt
     1081 tggatatacc tatttttcat tgttttttat tttattaaaa tcgcattaag taaagcgttg
     1141 agttatgagt attacctcaa attttttatt gctggaattt tattagtatt gatttggatg
     1201 atgcccgcca actttatctt tttagaaatt caccgtttaa cactgccctt actagtaatt
     1261 actttcattt cattaaagca aaaaccagca catgactagt gaaaaatcat accgttttca
     1321 ttttttaact attttaagcg gaaatacact aagtcagtta attccctttg taatcgcacc
     1381 gtttttaact cgtgttttta caacggaaga ttttggtgtt aatgccaatt ggttgtctat
     1441 cgttacgctt ataggtatca tttctacagg tcgtttagaa atggcgatac ctttgcccaa
     1501 aagcaacaaa gaggctagtc ggatattttc tagtggcttg atattcacca ttatttttac
     1561 tttattaagt ctattattaa tcgtttttga cgaccaaatt gctaattggt ataaagatcc
     1621 tgaattgaaa tattatttac aaatggcgcc aataggtgtt tttagtgtgg gattgctgaa
     1681 tatatttaac aattggtcct tgcgtcatag aaaatacagg agaatttctg ttggaaaagt
     1741 agttcaatct attgtaaaca atgcaggtgc tttgtttttg ggttatcttt cttttggggt
     1801 gtggggttta ttaattgcat gggttgtttc tcaattttta aatgcatttg tactcataga
     1861 aaaaggtgat ttaagctatt tgtttagaag aaaaaatttt aatacctcca atttcaaatc
     1921 aattgtttca aaatacaaag attttccttt agtaaatagt ttacatgcat ttaccgatat
     1981 ttttgccact caattcatct tgttttattt gattgccgtt tatttttcaa aagaggaatt
     2041 agggctattt tttttaatgc accgatttgt tagagctccc attgttttga tatctagctc
     2101 cgtttcgcag ctgtattacg tggaagccag caaagccata caacaaaact tatctggttt
     2161 gccgatggcc tttaaaacca tgaaaacagc aagtattttt gcaattcctt ttttaattgt
     2221 tttgctgttc tttgctccat ccattttttc atggtatttg ggaagcgagt ggagagaggc
     2281 gggcgtttat gcacaatatt tggcacccat gtttttttta agtttttttg tatctccatt
     2341 atcgggaacg ccgttaattt ttaaccgtca aaaaacttca tttattatca atctttgtgg
     2401 atatgtgctg accttaggct ccatatttct aggatatatg ctagaataca catttgcaga
     2461 aactttgata ttatacgcaa tatctttttc tttactttat tttgtacttt tagtttggta
     2521 tttaagttta cttagaaaat gagagtgata ataacaggac ttccattgtt ttcaaagcgg
     2581 atggcagatg atttgaatga atttacgcat tcaaaacagt tcatttattt gaatacctat
     2641 tattctaaat gggatcaaat aaaattcatt tgtttgttgc catttgttgc tggtgtggtt
     2701 tctatgaatg gcgtaacaag caaaagcgga attctagatt atgtttgctt atggagaaaa
     2761 aaaatatgga tgcaatggat gggaaccgac ctattgcttg ccaatgaaag atccaaaaat
     2821 aaaacaatca ataccaaata cattgaaaaa gcttgtcatt gggtagatgc tccgtggatg
     2881 caacaagaat tagcagacat ttctattgct tcaaaaacaa tcgattttaa gtttctctct
     2941 aatatcccaa caacattaaa aaaatataaa aaactggagg tctgcactta tattcctcaa
     3001 aacagacaag agttttatgg tatgcactac ctgtgtgaat tggcaaaagc acatccagaa
     3061 attgattttc atgtatatgg gactaaaaat cctgatgatt ttgtaccaaa aaatgtgatt
     3121 tgttgggact ggcaacccgc agatgttttg cacaaaaaaa tgagtgaaag tgcagtgttt
     3181 ttgcgattaa caaaacacga tggatattct gtaagtgtaa ttgaagccat gagtttaggc
     3241 tgtgaagttg tatcattttt tccttttgat aaagcacatg ttgttaccga aacttctatt
     3301 ttacctacct ttgctcgagt aattcaagag attaatgaaa gaggtttaac tcccaacttt
     3361 catcttcaag aatattatca gaataaatac aagaaaacta atgttctaac aaatttatac
     3421 aacgaattga aagactattt tgaaaaataa acaacatata gcattagtta ctacatggta
     3481 tccgcctaaa caaagcgttg caaccaatag aatgcttgca tttgcaacaa ttttagcaaa
     3541 cgacttttgc gttaccgtgt ttgcgctgga cgaaaaaact catactgtta aagaacacga
     3601 caatttatat gtaagatatt ccaaatcaaa atctattttt gattatttag aaaacaaaac
     3661 aacagattct agaagcattc attttctaaa aactggaatg aaagttatct tggctaaagt
     3721 tgttaaaaat ccgctcaaaa agtggcaaaa acgaacaact aaagatttga ttcaaacaca
     3781 ccagcaatcc ccatttgatc ttattatcag tagctttgcg cctcaagaaa cacatttagc
     3841 agttattggt tttaagaagc gttactctga agtaccttgg attgcagata tgcgtgatga
     3901 aatgtcaaaa aatccagaca tctcttctaa aacaagaaat gccttaattc aagttgagca
     3961 atacgtcaat tcttatgcta atgcaataac aactgttagc agtccgattc taaatgactt
     4021 caaaactatt tgtccaaaag taactcattt taaagaaatt aggaatggtt acaatcatga
     4081 attaacaccc ttaacgcatc aaaacaaggt gtttactttt ggatattttg gaagctttta
     4141 tggaggtaga aaacctgtca ttttttttaa agctttacaa cacatcctgg ataaaaacaa
     4201 agattttgaa ttcaaatttg taattgttgg cgctcataaa aactttgaaa ttcctgctca
     4261 agttgaaaaa tttgtagaaa ttaggccaat acaaccttac cttgaagctg taaagatgat
     4321 gatgcaaatg gatatgaacg taatcatcca acccagatct caacgaaaag gagtgttttc
     4381 tggaaaatta tttgattata tttcagcagc acgacctgtt ttagcgatgg tggacaaaga
     4441 cgatgtggca gctaaaatga ttttagattt tgatgctggt tatgttgccg aatgcgatga
     4501 tttggatgaa aatatttgtg ctattgaaaa ggcttttgat gattggaaat caggaatagt
     4561 taaaaaagct tctgaagaaa acagattgag cttacaccgt aaaaaccaag taaatgaatt
     4621 gaaattgtta atcaacacca tcttgtcatg aaagttgcac acataggttc caattctgtt
     4681 catgtttctc gatttattga agcacttaac actcatgctg tagaacaagt actcattgca
     4741 gaaacacctg ttgacattaa tttcaacatc caaacattat tggttgattt tcatacattt
     4801 aatccattta cactgctaag aaataagcgt ttagcaacca attttttaaa agaattcaac
     4861 ccagatatca tccacattca tcaagtaaat agaattgctt tttggattgc tctgattgct
     4921 aaaaaattaa acatccctgt tgttacaact gcgtggggca gtgatgtgtt gttgatgcct
     4981 caaaaaaatt ggctttatcg ttatttggtt accaaaacat tgcaaaatag taaactagta
     5041 acagctgatg cggatgttat gcttcatgca atgcaaaact tagttcctgc gggtgttcgt
     5101 tatgaaaaaa ttcaatatgg aatagatccc gttgagttga ctttaaataa agaaaatata
     5161 gtttattcaa acagattaca caagtcatta tatcgagttg ataaaataat cgaatatttt
     5221 gcagaagcta gtgcgcacga ctctaattat caattaatta ttgctgcaac tggtgatgaa
     5281 tctgaaaaat taaaaaacct tgttgttgac ttaaatattg aaaataaggt tgaatttgta
     5341 ggatgggtta atgctgagca aaaccgttat tggtattcaa aagctcaatt ttatgtttcc
     5401 atcccatcaa gtgacggtac ttctgtgagt ttattagagg ctatttcggc tggttgcatt
     5461 cctattgttt ctgatttacc tgcaaactta gagtgggtaa aggatgggaa tacagggata
     5521 attgaacaac caaatgaaaa tccaatatta aaagcatttc aatctcaatt tgacaatgcg
     5581 ctaatagaaa atgcacaaca cgttaacaaa ctggcgagta gatcatttac tgtacctcaa
     5641 tttataactt tttatcatga atgcatcagc tgttgaatcg aataaatatg cagtttggta
     5701 tccacgaatt attggattaa tagctttttt tgctttgttt gatttaaaag caaatgctat
     5761 attaaaacta aatgtattgc caattttaat tctaggtttt ttagctttgg ttatcaccgg
     5821 tattgtaaaa aaacaattga aatttaagtt aaaaccctta gctgttttat tgattgcatt
     5881 ttatgtttta tatgccattt atgccattta cactagaaac ccacatcaag cgggtgtgta
     5941 tcttgagaat aaattgtcgt ttttaatttt accactatta tttagtttta ggcctatttt
     6001 tacaatcaaa aaagatgcaa taatctatgg atggataggt ggaacactgg ctctcttttt
     6061 agcttctatc gtaaatggag tagcgtgtta tctcaattct gaagctaatt caagttgctt
     6121 tattagctct aatttttctt ttcaatacca tccaacgtat gcttctgtat attatactgt
     6181 ttcatttttc ttagcttatt atgcgtggaa aaacaaagta aaatggtttt ctgggtgggc
     6241 aacaattgca tacatgactg tgttttttat tgctgttttc ctttgtgtat ctctagcagg
     6301 catgctcttt tttatgttgg cactatccgt agcagtatta ctatttataa aaaataaatg
     6361 gggtaaaaaa tggatgataa tttctatgat tgttttgcca tttatgcttt atggtataat
     6421 caaaggcgtt ccaagaagtg aaggagaatg gtcaaacgct gtttggtatg cagagcaata
     6481 tatgaaagaa ccaagaactt atgttgaaaa atgtaaacca ccacattctg gaacacaaac
     6541 acgaataatc atgtggactg tagcttacca tgcttgtatg cactatccac ttggagttgg
     6601 aacaggaaat gttgacgaag ttcttgcaac ctatttgaga aaatacaacc aagaaaacat
     6661 ggtggaatac caatacaatc cgcacaacca atttttacaa acctggttgg aaatcggtgt
     6721 attgggattg ttgtgcttaa tagggattat cgtttacctt atgtacattg gcattcaaca
     6781 aaaaaactac ttaataattt tagttgcttt aagccttgca tttaattgct tgtttgaatc
     6841 tatgttgcaa cgtcaaagtg gtattttctt ttatccattt ttatttttta ttttagagtg
     6901 gttttcaacc aaaattaatc caacagacaa tcgtttatct gaataaatct atttttaatt
     6961 catgaaaaaa atcatcacca tacttggagc acgcccgcag tttatcaaag ctgcagctgt
     7021 ttctgctgta tttaaaaacc aagtaaacga gttgttggtg cacactgggc aacattatga
     7081 tgccaacatg tcggatgtgt tttttgaaga actcaacatt cccaaaccgc agtatcacct
     7141 caacattgga tcgggctcac atggcgaaca aacgggcgaa atgctcaaag caattgaaaa
     7201 agtaatacta gccgaacaac ctgattttgt aatggtgtat ggcgatacca attccacact
     7261 ggccggagct ttggcggctt ccaaattgtt gattcctgtc attcacatcg aagcaggatt
     7321 gagaagtttt aacaaagcaa tgcccgaaga acaaaatcgc attttaaccg accatgtgtc
     7381 gtcgttgtta tttgtgccaa cgcaaactgc tgtggacaat ctaaccaacg aaggaattac
     7441 taatggcgtg catttggtag gcgatgtgat gtatgatggc attcttcatt tctcgaaaat
     7501 tgctgaagaa aaaagcgata tattagaaaa ataccaactt gaaaataatc aatttgtgct
     7561 ttgcaccatt caccgtgcag aaaacaccaa cgatcccgca cgtttaaaag ctatttttga
     7621 aggtttaaat caagcaaaag ttaaaacagt acttccattg catccacgca cacaaaaata
     7681 cattgcagat tatggcatta cgttagaaaa ccacattcag gtgattgaac ctgtgggcta
     7741 tttggacatg gtgcgtttgg aaagtgcttg ttctaaaatc gttaccgaca gtggcggcgt
     7801 acaaaaagaa gcatttttct taggaaaacc gtgcatcacc atgcgcgatg aaaccgaatg
     7861 ggtggaaacc atagaaaatg gctggaatat tttagtgggc gctgatagct caaaaattgt
     7921 gaatgcattg caaacgttta acccaactgt tgaacgaaaa aattactttg gagatggtac
     7981 tgcctctcaa caaattttag cgcatatatt gaaataaaat ttacgcaaat gaatcaactt
     8041 tctgtagtta taccttgtag aaacgaagca ccatttatca ccgaatgcat tgaagcaatt
     8101 tatgcggcac aattgcccag tgataccgaa ttatctgttt ttgtagtaga tggaatgagt
     8161 acggatgcca ctcgcgagat agtactagaa ctcaccaatc gttatccttc attggcgttg
     8221 attgataaca ccaaacagtt aacacctttt gcgtttaatt taggaataca tgcaaaaccg
     8281 tgcgattatg tacaaatcat tggtgcacgt catattattt cagaaaatta tttaatcaac
     8341 aacttgcaaa cgttgcaaaa agacagttct atttggtgtg ttggtggcaa aataatcaac
     8401 gaatatgtga acccaacagg aaaagtaatt tcgttggcaa tggcaacaca attgggtatg
     8461 ggaattggaa attttagaac tttaaaaaat tcaggtttta ccgataccgt taccagcccg
     8521 atgtatcctt attgggtatt tgaaaaaatt ggcttttttg acgaagaact gattcgtaat
     8581 caagatgatg actttaatta ccgggtaaca aaagctggtg gaaaaatcta ttttgatgct
     8641 tctatttcat taaaatacta cgttcgtggc gattacaaaa atttatggaa acaatttttt
     8701 caatacggct attggaaagt ctttgtaaat aaaaaacaca aagcggttac aacctttcgc
     8761 cagttgattc cgcctttgtt tacattttac ttgctactca acgccctgtc gtggctaatt
     8821 ggtgggtatt ttgcttttat tggaaccttg cctttgtttt tatacttggg gctggttacc
     8881 tatgtttcat acgacattca acaatccaat aaagagctaa aattatggga tattttcaaa
     8941 acatttccaa ttttacatgt tggctatggc ttgggatatt tgaatggaat ttgggatttt
     9001 atcatcctta aaaaatctcc ttccgaaaaa caaaaaatgc ttagtcgcta gtttttataa
     9061 tattttttgt aaattcacat aaatttaata ccatgaaaaa gattaattta atagtatttg
     9121 ctgcactact tttagttttt agtgcgtgtc aaaaatacga agaaggacct gctttttctt
     9181 tgttaacagc aaaaacaaga ttggtaaacc attggaccat tgaaacctat gatattgatg
     9241 gtgttaacca accaataaat gtaaacacca atttggaaat ggattttttt aaagacaata
     9301 cctttaaaag aacatggacc atttttggct ttatgacagc agaagaagga gattgggaat
     9361 ttaccaacaa caaactcaat gttttattaa agaaaaagga tggaaatatg gagttataca
     9421 aaattgtgaa attaaccaat aatgagctca aagcaaaata caccgatcaa aatggtaaaa
     9481 cacactccta ttatttcaaa gggaaataaa gaattgaaga aataaaaaaa ggacggataa
     9541 cccacgaatg gtcggaagaa atttcttccg acttttttgt atttattaac ctttaa
//
LOCUS       DAOQVQ010000035         9538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8009_length_9538_cov_4.382052, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000035 DAOQVQ010000000
VERSION     DAOQVQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8009_length_9538_cov_4.382052"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKN22_02055"
     CDS             complement(<1..993)
                     /locus_tag="PKN22_02055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNS41515.1"
                     /translation="MMTLLKKTLKFVFYLLLVLLIVANGFILLSGRTYLYRGIYLTYL
                     SGKTGPSIYDLDNFPKRKVAKGNKQLPWIKTANYTQNTQINGFEQYNEGLETKAFLIF
                     KNDSLLFEKYWGNHTVSTLSNSFSAAKTMVALLIGIAVEEGKIKNLDEPVGNYLPEFK
                     RDDLSIITIRDLLLMASGLNWSESGKNPLSNNAESYYGTDLYGLVTRQKRISTPGRTF
                     IYQSGNSQLLGYIIQKATGKNVSDYCSEKLWSKMGMDADAYWSLDKENGDEKAFCCLY
                     ATARDFGKIGQLIANRGKWGNEQLIPSWYFEEMVSAPEDMDTEEAIKNYQYGLHI"
     gene            1240..1599
                     /locus_tag="PKN22_02060"
     CDS             1240..1599
                     /locus_tag="PKN22_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41516.1"
                     /translation="MLAIILIALNNNASQYFLNYEDFPHVEAGFNIQNGTDNKNNVVY
                     TDNDENQLTLAQEFFKEKEGEAESDLFSHLFLNDFTLPSTAVLNDFTSKKLIIANYIQ
                     EAKGQVPLWIKNCQLLI"
     gene            1704..2780
                     /locus_tag="PKN22_02065"
     CDS             1704..2780
                     /locus_tag="PKN22_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS41517.1"
                     /translation="MKKMSMVTCFAALLLMVGCKSEEHTEEQSKFEVTNPIQKDTVVY
                     KNYVCVINSIQHIELRALEKGYLQNIYVDEGQFIKKGQLMFKILPTIYEAEVSVAKAQ
                     KEFEEIEYQTTQRLADKKIVSPNELAMSKAKLEKAKAELLLANTHLGFTDVRAPFDGY
                     MGRFNVRLGSLLDEGELLTTLSDNSKVWVYFNVPEAEYLHYKQHNEKMPKVKLLMANN
                     QFFDKEGEIETIAADFNSETGNIAFRATFDNSKGILRHGETGSILVPDDIKDAVIIPQ
                     AATFEVLDKKYVYVVNKNNVIHPREIQIEAELPQLYVVSKGLSTKDKVLLNGLRKVKN
                     NDKIQYTYRKTTDVLKEMNSLFSE"
     gene            2797..6027
                     /locus_tag="PKN22_02070"
     CDS             2797..6027
                     /locus_tag="PKN22_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS41518.1"
                     /translation="MFSKFIKRPVLALSLSLAILFLGFLAINTRPISQFPEIAPPQVN
                     VFIAFPGASANVLVKSTLVPLERAINGVQGMRYIVSDATSAGEATIQIIFEPGTNPSD
                     AVVNVKTRIDQVMNNLPPLVQREGVIITPIQPSMLMYVNLYSKDKGADEKFLYNYANG
                     TLLPEIQRIHGMGRAQILGSRKYAMRIWLKPDRMRAYKVSTDDVMEAINEQSVIGRPG
                     RLGQSTGKQAQSLEYTLSYKGRFNKPEEYENIIIRANADGEVLKLKDIASVEFGSEFF
                     DIYSNKDGYPSASIVLKQNIGSNASEVIEQTKEKLKELKQSFPAGMDYEINYDVSKFV
                     DASIDKVIHTLVEAFILVAIVVFIFLGDLRSTLIPIIAVPVSLVGAFIFMQLFGLTIN
                     LITLFALVLAIGIVVDDAIVVVEAVHAKMEEKHLSPFKATKEVLEEISGAIIAITLVM
                     TSVFVPISFMSGPVGVFYRQFSITMASSIVLSGLVALTLTPVLCAMILKNNHGKPKRK
                     TPITMFIDFFNRGFEKLTGRYTNVLHKIVTRKTVTWATFLLFGFGIFMVNKTLPTGFI
                     PNEDQGMIYAIVQTPPGTTLERTNEISRQLQDIAEKIEGVQSVTSLAGYEVLTEGRGS
                     NAGTCVINLKNWEDRKNNVHKVIEELEEKTKDIGTVVEFFEPPAVPGYGSSDGFSLRL
                     LDKAEETDYQEFSKINDEFMAALHKRKELAGLFTFYAANYPQYDLEIDNLRAMQKGVS
                     IGKAMDNLDILIGSTYEQGFIRFNNFYKVYTQASPEYRKMPSDVMNLFIKNEEGEMVP
                     YSSFMTMKKTQGPNEITRYNLYTSSAIRGVPAPGYTSGDAIEAIKEVAKETLPKGYDI
                     AWEGLTYTEAARGNEAVVIFIVVILFTYLVLSAQYESFLIPLVVILSLPMGIFGSFMM
                     LKLMGLANDIYAQIGLIMLVGLLGKNAILIVELAIMKHREGLTVFESAIEGAKARFRA
                     ILMTSSAFIAGLIPLVVAHGPGSVSNHTIGSSALGGMLTGTIFGVIIVPGLYYIFGTL
                     AEGKYYIKDQEDAPLSEDFVEYVEPKTKKKRAINSLIKKITKKNDD"
     gene            6017..7465
                     /locus_tag="PKN22_02075"
     CDS             6017..7465
                     /locus_tag="PKN22_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNS41519.1"
                     /translation="MMTKFKSKWMVIALCFVVLGCKSSKTALQDETYKQGKDYIPTAY
                     NAAQTQDTNTIAAKNFKAVFSDIHLTTLIDSALHNNQELNITMQEIEVAKSEVRAKKG
                     EYLPTGGLQLGAGLDKSAEFTRNGAVESQLHVMEDKKFPEPLGDFMVGAHFSWEVDIW
                     RKMRNSKNAALSRYLGTIEGRNFMVTNLVSEIAENYYKLLALDEQLTIVNHFIDIQED
                     VLEIVQQQKDAAKVTELAVNRFEAQLLKTKNMQYSIKQSVVETENTINFLVGRFPQPV
                     ERDITSFHSTILDTIVTGSPEQLLSYRPDIRKAERDLVAAKYDVKAAKAAFYPSLGIS
                     ANVGMQAFNPAVWFNPKSLLYNLLGDLFAPLINRNAIRAQYNASKAKQSQALVDYQQK
                     ILKAFIEINNQLAMVKNFSESLQTKTKEVDILNQSVDISNDLFMSARADYMEVLLTQK
                     EALDAKMELIETILKEKEAKIGIYRALGGGWK"
     gene            7599..9290
                     /locus_tag="PKN22_02080"
     CDS             7599..9290
                     /locus_tag="PKN22_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41520.1"
                     /translation="MCLPKKFIYSLLFFLLLYPSFPTNANEPLLNKSYAERQPLLIER
                     FYYHSDKKDSVRYFNEIKRLRQLAIQYNDRELELETDYMKLNYLSSRGYKNYISELNQ
                     LMQKADDEHILQLQIRTRQALGFHYFYEYRYDGKALFWFLESYNFLSKIKQEDFPEKK
                     NALLDIANMCLHTGYYQKALFYINEAQKIEFSYDKNLHLNLLNTKALIYQNSGNNEKA
                     TIELENLLKKAHNENNDIWFLIASTNLAKLYIKTKDYTNASRIVAACEKMNSDSFPNE
                     YFEFLLVKANLLYQLDPHNTFIHFIEANLLDKNIQQLTLNSKTDYFHLLYYYYLIKND
                     AQQIINFANLYIEHKDSLTQIITDNSIKQAAEKHNYNRYVANKRLIEQTRYKNKRDTV
                     IFIVAILFILATVAVVFRKRKKRIEIEKNKYIKQIEQQRVLVENAKKQIDQFIADIQQ
                     KNGVIQKLQNELSPNSIELLKQTTILTDEHWLNFKKEFSIVYPEFFININKQQQLTPS
                     LERLAALIVLDLDSMQIGQTLGISKESVARSKRRLKAHFNLTKDNDLLQTLKKHL"
BASE COUNT         3241 a         1594 c         1735 g         2968 t
ORIGIN      
        1 aatatgcaag ccgtattggt aattttttat tgcttcttca gtgtccatat cctctggagc
       61 acttaccatt tcttcaaaat accagcttgg aattaattgt tcatttcccc attttccacg
      121 gttggcaatt agctgaccaa tttttccaaa atcacgtgct gtggcataca aacagcagaa
      181 tgctttttca tccccatttt ctttgtctaa actccaataa gcatcggcat ccattcccat
      241 ttttgaccat aatttttcag aacaataatc cgaaacgttt ttgccagttg ccttttgaat
      301 aatgtagccc aataattgac tgttgccact ttgataaata aatgtccgac caggtgtgct
      361 gatacgtttt tgacgggtta cgagtccata taaatcggtg ccatagtacg attctgcatt
      421 gttggacaac ggatttttac cactttccga ccaatttaat ccagaagcca ttaacaataa
      481 atcacgaatg gtaatgatgg ataaatcatc ccgcttaaac tcgggtaggt aatttccaac
      541 gggttcatct aagtttttta tcttcccttc ttcaaccgca ataccaatca gcaatgctac
      601 catcgtttta gctgcagaaa atgaatttga aagcgttgaa acagtatgat tgccccagta
      661 tttttcaaac aacaatgaat cgtttttaaa aatcaaaaat gcttttgttt ctagcccttc
      721 attgtactgt tcaaagccat ttatttgcgt attttgggtg taattggccg tttttatcca
      781 aggtagttgc ttatttcctt tagccacttt tcgtttgggg aaattatcta aatcgtatat
      841 ggaaggtcct gttttgcctg ataaataggt taaatatatt ccacgataca aataagttct
      901 tccagatagt aaaatgaaac cattggcaac aatcaaaaga acgagaagta aatagaaaac
      961 aaatttcaac gttttcttca ataaagtcat catggttttc atagaggtat attttgacag
     1021 ttaatttaca ctttattttt tagaagccaa agagatttta agaattatta tttttgatta
     1081 ataatttatt acttcaatca taaatattca gaaaaataga tttcaaatgt taaatttcgt
     1141 gttaaatatt acgaatcact tgttttgcaa ctttaaaaat agtcaatttt gaggtcaaat
     1201 atgagatgtt ttagattaca tattggtcgc attttttgca tgttagcaat tatacttatt
     1261 gctttaaata acaacgcatc tcaatatttt ttaaattacg aagattttcc acatgttgaa
     1321 gcaggtttta acattcaaaa tggtacagac aataaaaata atgttgttta taccgacaac
     1381 gatgaaaatc aactcacatt agcacaagaa ttttttaaag aaaaagaagg tgaagcagaa
     1441 tcagatttgt tttctcacct ttttcttaat gattttacac taccatctac tgctgtttta
     1501 aatgacttta cttctaagaa attaataatt gcaaactata ttcaagaagc taaagggcaa
     1561 gttcctctct ggattaagaa ttgccaactc ttgatttgat ttgatttagg ttttaaagat
     1621 tgttttttaa gtcaatctca tttttggtgt tgctattaga gcaacaaatt catgcaattt
     1681 attaattaat ttctgtttat tttatgaaaa aaatgtcaat ggtgacatgc tttgcggcat
     1741 tgttgctaat ggtgggatgt aaatcggaag aacacaccga agaacagtca aaatttgaag
     1801 ttactaatcc cattcaaaaa gacacagtgg tgtacaaaaa ctatgtgtgt gtcatcaatt
     1861 ctattcagca cattgaattg cgcgctttgg aaaaagggta tttgcaaaat atctatgtag
     1921 atgaggggca attcatcaaa aagggacaat tgatgttcaa gattctacct actatttacg
     1981 aagcggaggt aagtgttgca aaggcgcaaa aagagtttga agaaatcgaa taccaaacaa
     2041 cgcaacgttt ggccgacaaa aaaatcgtgt cgccaaatga attagcaatg tcaaaagcta
     2101 agttagagaa agcaaaagca gaattattac tagccaatac tcaccttggt tttaccgatg
     2161 tgcgtgcacc ctttgatggt tacatggggc gttttaatgt gcgtttggga agtttgttag
     2221 acgaaggtga attgctgacc acgctttctg ataacagtaa agtttgggtt tatttcaatg
     2281 ttccagaagc cgaatatttg cattacaaac aacacaacga gaaaatgcca aaagtgaagt
     2341 tgttaatggc gaataatcaa ttttttgaca aggaagggga gatagaaacc atcgctgccg
     2401 atttcaatag tgaaacagga aatattgcat ttagagcaac ttttgataac tcgaaaggaa
     2461 ttttacgcca cggtgaaaca ggaagcattt tagtgccaga tgacattaaa gatgcagtca
     2521 tcattccgca agcagctacg tttgaagtac tcgataaaaa atatgtgtat gtagttaaca
     2581 aaaacaatgt tattcatcca cgagaaatcc aaatcgaggc agaattaccg cagttgtatg
     2641 ttgtttctaa aggcttgtca actaaagata aagtcttgtt aaacggattg cgaaaggtaa
     2701 aaaacaacga taaaattcaa tacacttatc gtaaaacaac ggatgtattg aaagaaatga
     2761 attctttgtt ttcagaataa tctaaattaa gttaagatgt ttagtaaatt tataaaacga
     2821 ccagtattag ccttgtcgtt atcgctagcc atcctttttt taggatttct agccatcaac
     2881 actcgaccaa tttctcagtt tccagaaatt gctcctcctc aagttaacgt atttattgcc
     2941 tttccgggtg caagtgccaa tgtgttggta aaatctacct tggttccgct tgaacgtgcc
     3001 atcaatggtg tgcaaggaat gcgttatatt gtttcggatg caaccagtgc tggtgaagca
     3061 accattcaaa ttatctttga accaggaacc aacccaagtg atgccgttgt aaacgttaaa
     3121 acaagaattg accaagtaat gaataattta cctccgttgg tgcaacgtga aggagttatc
     3181 attacaccca ttcagccaag tatgttgatg tatgtcaact tgtatagtaa agataaaggt
     3241 gctgatgaaa aattcttgta caactatgcc aacggaacgc ttttgccaga aatacaacgt
     3301 attcacggta tggggcgtgc tcaaattttg gggtcacgta aatatgcaat gcgtatttgg
     3361 ctaaaacccg acagaatgcg agcttataaa gtttctactg atgatgtgat ggaagccatt
     3421 aatgagcaaa gtgttattgg tcgccctggt cgtttaggac aaagtacagg taaacaagct
     3481 caatcgttag agtacacgct ctcctacaaa ggtcgtttta acaagcctga agaatatgaa
     3541 aacatcatca ttcgtgccaa tgccgatgga gaagtgttga aattaaaaga cattgcttcg
     3601 gttgaatttg gaagtgaatt ttttgacatt tattctaaca aagatggtta tccttcggcg
     3661 tccattgtct tgaaacaaaa tattggtagt aacgccagtg aggtaattga gcaaaccaaa
     3721 gagaaattaa aagaattaaa acaaagtttc cctgcaggaa tggattatga aatcaattac
     3781 gacgtatcta aattcgttga tgcatccatt gataaagtaa ttcatacctt ggtagaagcc
     3841 ttcattttgg tagccattgt ggtattcatt ttcttaggtg atttacgttc tacattgatt
     3901 ccaattattg ccgttccagt ttccttggtt ggtgcgttta tttttatgca gttgtttggg
     3961 ctaacaatca acttaattac gctctttgca ttggtgttgg ctattggtat tgtggtggat
     4021 gatgccattg tcgtcgtcga ggcggtgcat gccaaaatgg aagaaaagca tctatcacca
     4081 ttcaaagcca caaaagaagt attggaagaa atcagtgggg caattatcgc catcaccttg
     4141 gtaatgactt cggtgtttgt gcctatttcc tttatgtcgg gaccagttgg ggtgttctat
     4201 cgtcaattct ccatcacaat ggcaagttcc atcgtgcttt ctggtttggt tgccttgacg
     4261 ttgactcccg ttttatgtgc catgattttg aaaaataatc acggtaaacc aaaacgtaaa
     4321 acgccaataa caatgtttat cgactttttt aaccgagggt ttgaaaagct aacaggtcgc
     4381 tataccaatg ttttacataa aattgttact cgcaaaacgg taacatgggc aacatttctt
     4441 ctttttggat ttggaatttt tatggtcaat aaaacattac caacaggttt cattcctaat
     4501 gaagatcaag gaatgattta tgccattgtg caaacacctc cgggaacaac gttggaacgg
     4561 accaatgaaa tttcacgcca attgcaagat attgctgaaa agattgaagg tgtgcaatcg
     4621 gttacatcac tagcaggtta tgaggttttg acagaaggcc gtggctccaa tgcgggtact
     4681 tgtgtcatca acttaaaaaa ctgggaagac cgaaaaaata acgtacacaa agttatcgaa
     4741 gaattagaag aaaaaaccaa agatattggt acggtggttg agttttttga accacctgca
     4801 gttccaggtt atggttcgtc tgatggtttt tcattgcgtt tattggataa agcagaagaa
     4861 acagattatc aagaatttag caaaatcaac gatgaattca tggcagcatt gcataaacgt
     4921 aaagaacttg ctggtttgtt tacattttac gcagccaatt atccacaata cgacttagaa
     4981 atagacaacc ttcgcgccat gcaaaaagga gtttctatag gcaaagcaat ggataacttg
     5041 gatattttga ttggttcaac ttatgagcaa ggttttatcc gtttcaacaa cttctataaa
     5101 gtgtatacgc aagcatcgcc tgaatatcgt aaaatgcctt ccgatgtgat gaatttgttt
     5161 attaaaaacg aagaaggcga gatggttccg tattcttcat ttatgaccat gaaaaaaaca
     5221 caaggtccaa atgaaattac acgttacaac ctttatactt cttcagcaat tcgtggggtt
     5281 cctgcacctg gctacacttc gggtgatgca attgaggcca tcaaagaagt agcaaaagaa
     5341 acattgccaa aaggttacga tattgcttgg gaagggttaa cctataccga agcagctcgt
     5401 ggaaatgaag cagttgtcat atttatcgtc gtaattctat ttacttactt ggttttatcc
     5461 gctcaatacg aaagtttctt aattccgttg gtggtgattt tatccttacc tatggggatt
     5521 tttggttctt tcatgatgct taaattaatg ggattagcca acgacattta tgcacaaatc
     5581 ggactcatta tgctggtcgg attgctgggt aaaaatgcca tcctgattgt agagcttgcc
     5641 attatgaagc atcgagaagg attaacggtt tttgaatcgg caattgaagg tgcaaaagcc
     5701 cgatttagag ccattttgat gacttcatct gcatttattg cggggttaat tccgttagtt
     5761 gttgcccacg gacccggttc tgtaagtaac cacactattg gttcttcggc acttggtgga
     5821 atgttgacag gtaccatctt cggggtgatt attgtgccag gattgtatta catttttggg
     5881 acattggcag aaggaaaata ctacataaaa gatcaagaag acgctccatt gagtgaagat
     5941 tttgtggaat atgtagaacc taaaaccaaa aagaaacgag ctatcaacag tttaattaaa
     6001 aaaattacaa aaaaaaatga tgactaagtt caaatcaaaa tggatggtaa ttgccttgtg
     6061 ttttgttgtt ttggggtgta aaagctccaa aacagcattg caagacgaga cctataagca
     6121 agggaaagac tatattccaa cagcttataa tgctgctcaa acacaagata ccaatacaat
     6181 tgcagcaaaa aatttcaaag ctgtattttc ggatatacat ctaactacgt tgattgattc
     6241 agcattgcac aacaatcagg agttaaacat caccatgcaa gaaattgaag tggcaaaaag
     6301 tgaagttcga gctaaaaagg gagagtattt gccaactgga ggcttgcaat tgggtgctgg
     6361 attggataaa tcagccgaat ttacacgcaa tggggccgtt gaaagtcaat tgcacgtaat
     6421 ggaagacaaa aaattcccag aaccattagg cgattttatg gttggagcac atttttcgtg
     6481 ggaagtggac atttggcgca aaatgagaaa tagtaaaaac gctgcattgt cacgctattt
     6541 aggcactatt gaaggacgaa acttcatggt aaccaaccta gtttcagaaa ttgcagaaaa
     6601 ttattataag ttacttgcct tggatgaaca attgacaatt gttaatcatt ttatcgatat
     6661 tcaggaagat gtgttagaaa ttgttcaaca acaaaaagat gcagccaaag taaccgaact
     6721 tgctgtaaat cgttttgaag cacaattgct aaaaactaaa aacatgcaat acagcatcaa
     6781 acaatcggtg gtggaaacag aaaatacaat caattttttg gtggggcgtt ttccgcaacc
     6841 tgtagagcga gatataacat cgtttcatag cacaattttg gatacaatag ttaccggttc
     6901 gcccgagcaa ttattatcct accgccctga tattcgcaaa gcagaacgcg atttggttgc
     6961 agcaaaatat gatgtaaaag cagcaaaagc agcattttat ccttcattgg gtatttcagc
     7021 aaatgtagga atgcaagctt ttaatccagc ggtgtggttc aatcctaaat cgttgcttta
     7081 caatttactg ggcgatttgt ttgctccact tatcaataga aatgcaatac gtgcgcaata
     7141 caatgcctct aaagcaaaac aatctcaagc attggtggat tatcaacaaa aaattctaaa
     7201 agcctttatt gaaatcaaca atcaattggc aatggtgaaa aacttttcag agagtttgca
     7261 aacaaaaaca aaggaagtgg atatcctcaa tcaatcggtt gatatttcta acgatttgtt
     7321 tatgtcagcc agagccgatt acatggaagt gttgttgaca caaaaagaag ccttggatgc
     7381 caaaatggaa ttaatagaaa caatcttaaa ggagaaagaa gctaagatag gcatctatcg
     7441 tgctttaggt ggcggttgga aataaataat gtttggttta atgttaatag ccgtttgtct
     7501 gaaaaggcaa gcggtttttt tgtgtttatg tttcagtttt tagtttaaaa tctaaaaact
     7561 taactatatt tgaaatgaaa taaaaagtat ataattaaat gtgtctgcca aagaaattca
     7621 tttacagttt actatttttt ctactacttt atccaagctt tccaacaaat gctaatgagc
     7681 ctttattaaa caaaagttat gcagagagac aaccactgct aattgaacgt ttttattacc
     7741 attccgacaa aaaagattct gttcgttatt ttaacgaaat aaaacgtttg aggcaattgg
     7801 cgatacaata caacgacaga gaattggaat tggaaacaga ttacatgaaa ttaaactacc
     7861 tttcatctag aggctataaa aactacattt ccgagttgaa tcaactgatg caaaaagcag
     7921 atgatgagca tattttacaa ctccaaatta gaacacgaca agcactaggt ttccattatt
     7981 tttacgaata ccgttacgat ggaaaagcat tgttttggtt cttggaatcc tataattttt
     8041 tatccaaaat aaagcaagaa gattttccag agaagaaaaa tgccttattg gacattgcca
     8101 atatgtgttt gcatactgga tattatcaaa aagctttgtt ttatatcaat gaagctcaaa
     8161 aaattgaatt tagttacgat aaaaacctac atttaaacct acttaataca aaagcactta
     8221 tttatcaaaa tagtggaaat aatgaaaaag ccaccattga attagaaaac ctactcaaaa
     8281 aagcgcataa tgaaaacaac gatatttggt ttttaatagc atcaactaat ttagcaaaac
     8341 tctatataaa aacaaaagat tacaccaatg ctagccgaat tgttgctgct tgtgaaaaaa
     8401 tgaatagtga cagttttccc aatgaatatt ttgagttttt attggtaaaa gcaaatttac
     8461 tttatcaatt ggatccccac aataccttta tacatttcat tgaagctaat ttattggata
     8521 aaaacataca acagttaacg ttgaattcaa aaacggatta ttttcacctt ttgtattact
     8581 attatttaat caaaaatgat gcacaacaaa ttataaactt tgccaatctt tatatagaac
     8641 acaaagatag tttaacacaa attattaccg ataattcaat aaaacaagct gcagaaaaac
     8701 acaattacaa ccgctatgtt gccaataaac gattaattga acaaacacgt tataaaaaca
     8761 aaagagatac tgttattttt attgttgcga tactttttat acttgcaaca gttgcagttg
     8821 tgttcagaaa acgtaagaaa agaatagaaa tagaaaaaaa taaatacatt aaacaaattg
     8881 aacaacagcg tgttctagtt gaaaatgcta aaaaacaaat cgaccaattt attgcagata
     8941 ttcaacaaaa aaatggagta attcaaaaac tacaaaatga actttctccc aatagtattg
     9001 aacttttaaa acaaacaaca attcttaccg atgaacattg gttgaatttc aaaaaggaat
     9061 tttcaattgt ttatcctgaa ttttttatca atattaataa acaacagcaa ctcacaccat
     9121 ctcttgaacg attagcagct ttaattgttt tggatctaga cagtatgcaa attgggcaaa
     9181 ctttgggtat aagtaaagag agcgttgccc gctcaaaaag acgtttaaaa gctcatttta
     9241 acttaacaaa agacaatgat ttgctgcaaa ccttgaaaaa acatctttag atttcatttt
     9301 caaataattg atagttaaat cattataaat tgtccgcttt ttgtccgcat aatttttttt
     9361 taatagtata tcatgctaat acatttgcca acaattttca aataaccaat taaaagtttg
     9421 ttgaatattt tatctatttc atactgctta tgaaaaagct actactatca tgttaaatta
     9481 aaattcagca ttcaaaagcg actttaattt ggtcgctttt ggtgtttttg agagttgg
//
LOCUS       DAOQVQ010000036         9512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8039_length_9512_cov_3.346939, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000036 DAOQVQ010000000
VERSION     DAOQVQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8039_length_9512_cov_3.346939"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1448
                     /locus_tag="PKN22_02085"
     CDS             174..1448
                     /locus_tag="PKN22_02085"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNS41521.1"
                     /translation="MKVDVLLGLQWGDEGKGKIVDVLTPQYDVIARFQGGPNAGHTLE
                     FEGIKHVLHTIPSGIFRKEVVNVVGNGVVIDPVIFNGELEKLKPFNIDYKKTLLLSKR
                     AHLILPTHRLLDQASEQAKGKNKIGSTLKGIGPTYMDKTGRNGLRVGDIIAPNFKEKY
                     DALVEKHLGILKHYEDFTFDLATLEKPWFEGIEVLKQLEIIDSEHYINNALKAGKKVL
                     AEGAQGTMLDVDFGTYPFVTSSNTVTAGTCTGLGVAPTKIGSVIGIFKAYCTRVGSGP
                     FPTELNDEVGEKIRKDGHEFGATTGRPRRTGWIDLPALKYAIMINGATELSMMKADVL
                     DDFETIKVCTHYIINGEKVDFFPFDINDVEVTPVYEEVKGWNCSLIKLTNFADAPTEL
                     KDYVKYLEQKLEVPITVVSVGPDRTQTLNTKN"
     gene            1502..3088
                     /locus_tag="PKN22_02090"
     CDS             1502..3088
                     /locus_tag="PKN22_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNS41522.1"
                     /translation="MCFHVFSQAFGELLPGSSALIYDESKGIHKLLDGANFVYQGNTM
                     YCDSAYYIEKDKTVRAYGHVNIRKSDNVSLFCDSLWYHSSNGLAYLWGHVRANDAQYR
                     LTTDSMVYNTKTEQGIYRNGGKIENMLQYEVLTSKIGYFQPNSKNFFFRQNVVYKSDQ
                     LRMTTDSLQYKYEKKTCYFYGPTCIVTDDAFLEGEKGWYNVDTEEGSIAKNAVIEQPN
                     RIIKGDSLYYSPKTKIAEGFNNVSVLDTVDKIAFQSDYALMNDSLHYSLLTKNAILTK
                     FTQKDTLHIHADTLYNLNDSLNKRVQTLGYHNVRIFSSKVQGIGDSLSFTKKDGYMEL
                     YKKPMLWSKNGELKGDSIRVYMNDSIVDRARIWGKATAVMELDSGNYYNQLGGKEILA
                     HFEKNEVKTVKVMGNAQTVFFPEEEKGLENVPLEDSLVSLKDTSDFSNLALQSDSLAV
                     DSVPMKQVFVIKRMGMNRFYASEVKVYLDSGEVVGVTYYEKPDGVFYPMDQINKEEQF
                     IQNFSTNFALRPKSFADLLK"
     gene            3115..4281
                     /locus_tag="PKN22_02095"
     CDS             3115..4281
                     /locus_tag="PKN22_02095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNS41523.1"
                     /translation="MQNFEAYNPTRIVFGKNQYHRIAELAAENKATKILVVYGGGSIK
                     KNGVYEQIMQHLHAFQVVEFSGVGANPEYTTLMQAVELARKEKIDFILAVGGGSVIDG
                     VKFIAAAIHYSGNAWDVLMKKPGCKFGAAVPFGVVLTLPATGSEANSGAVINRSEMKE
                     KRTMGGPNLFPQFSICNPGVVASLPQRQIANGIVDSFMHTLEQYLTYPSGNLLQQRHA
                     EGILQTLIEIAPIVLQNPSNYEAAANLMWCSTHALNGNLRAGVPTDWCTHMIGHELTA
                     FYGIDHAQTLAIIAPRLFENQFENKLEMLVQYGKRVWNLEGTATEIAQQAIVKTENFF
                     QQVGIKTRISDYTTDTSFIAQEIKNRFIERGWTEMGERKAITPNDVYEIVEKSI"
     gene            4391..5104
                     /locus_tag="PKN22_02100"
     CDS             4391..5104
                     /locus_tag="PKN22_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNS41524.1"
                     /translation="MSDAVVIIPTFNEKENVERMTRKVMSLDKEFDILFVDDASPDGT
                     ASIIKQLMTEFPNRVFIEERTGKLGLGTAYIHGFKWAIAKKYDYIFEMDCDFSHNPDD
                     LIRLYNACAIDGADFSIGSRYVAGGKVENWPLKRLLMSYFASVYVRLILFINIKDTTA
                     GFKCYSRKVLEAIHLDGNTFKGYAFQIAMKYAALKHGFKVKEVPITFIDRVYGTSKMS
                     TGIFKEAFFGVWKMRRMKL"
     gene            5101..6438
                     /locus_tag="PKN22_02105"
     CDS             5101..6438
                     /locus_tag="PKN22_02105"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNS41525.1"
                     /translation="MKNYLIKNASIVNEGQTINGDVFIKNGIIEKVGSNLTVTENHEV
                     IDINGAYLLPGCIDDQVHFREPGLTHKGTIASESRAAVAGGITSFMEMPNTVPNALTQ
                     ELLEDKYAIASKTSIANYSFFMGASNENLDEALKTNPKNVCGLKVFMGSSTGNMLVDN
                     EKTLDNIFSKIPFLIATHCEDESTVRANKEHYQAIYGDEIPVELHPIIRSEEACYLSS
                     SLAVSLAKKHGSRLHILHISTAKELDLFDNSIPLEQKKITAEACVHHLWFSDEDYKTK
                     GNFIKWNPAVKKASDRAAIWQAVLDNRIDVIATDHAPHTIEEKMLPSYFQSPSGGPLV
                     QHALVAMLEKVQQGVLSIEKVVEKMAHAPAILFQINKRGFIREGYHADLVVVEKQPNV
                     VDKPSILYHCGWSPFEGVTFSHKVTKTFVNGNLVWNNNQLVEGSLGSRMLFDR"
     gene            6435..6899
                     /locus_tag="PKN22_02110"
     CDS             6435..6899
                     /locus_tag="PKN22_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNS41526.1"
                     /translation="MMRWSYIFLLTLGLFACKPTVEVGAEKPATLLTKEQMVAILTDL
                     TIMESVYQQKYIQVPRFSYLMIKEADSIFKKHGTSQLVFDANMDYYSADLEALESIYQ
                     QVKSNLIAQQNALPKVETLPQDDSSKVNKKALNGLPFHTEESMQHQEANKNK"
     gene            6925..7893
                     /locus_tag="PKN22_02115"
     CDS             6925..7893
                     /locus_tag="PKN22_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNS41527.1"
                     /translation="MTVWIIFFALITFFLALDLGVFHKKDAVVTIKESLIWTAIWVAV
                     ALLFGIAIYFIYENQLFGLNTHGINGIDAMTEYYTGYVLEESLSMDNIFVIAMIFKFF
                     KIQPKFQHRVLFWGIIGAVVFRLIMILLGQAFIEQFSWSMYVFGGILIWSAYKMLKDE
                     DEESDFKESLGVRILSKIYPINWKIQNGHFIEKIDGKTVATPLLATLIVIEFTDILFA
                     VDSIPAIFSITTDPFIIFSSNIFAILGLRSLYFFLSGMLDKFEHIKTALVFVLMFVGL
                     KMIFHDYLHFLSEHTWISLVVILTFLSGGVIASIISNKRKEKENLG"
     gene            7986..8534
                     /gene="pyrR"
                     /locus_tag="PKN22_02120"
     CDS             7986..8534
                     /gene="pyrR"
                     /locus_tag="PKN22_02120"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNS41528.1"
                     /translation="MEKQIILNKRHIELTLKRLCYQLIESHNDFSNAIIIGLQPRGIH
                     VVTRLKKELEAILNKEIVCGNLDITFYRDDFRRRERPLIPSVTNIDFDIEGKNIILVD
                     DVLYTGRTIRAGLDALLAFGRPSKVELLAFIDRRFNRDLPIQADYIGVNVDTLSQERV
                     SVEWNEIEGEDKVILFTPENNG"
     gene            8527..9456
                     /locus_tag="PKN22_02125"
     CDS             8527..9456
                     /locus_tag="PKN22_02125"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNS41529.1"
                     /translation="MDNLSVDHIIGIKDLTKADIELILKTAETFKQVINRPIKKVPSL
                     RDITIANLFFENSTRTRLSFELAEKRLSADVVNFSAANSSVKKGETLVDTTNNILSMK
                     VDMVVMRHPNPGAAKFLSERVNARIINAGDGTHEHPTQALLDAFSIREQLGTLEGKKV
                     VIVGDILHSRVALSNIYCLQKMGAEVMVCGPTTLIPKYISELGVKVEHNLRKALEWCD
                     AANMLRIQLERQDIKYFPSLREYSMLFGLDKKLLDSLDKKIVIMHPGPINRGVEITSD
                     VADSDQSIILQQVENGVAVRMACIYLLASKIER"
BASE COUNT         3154 a         1523 c         1973 g         2862 t
ORIGIN      
        1 ggaattaact tttttataaa agcattaaca aaatccagaa tacaccttgg cgacatggtg
       61 ttgaacggag caaaaggaga agtgttgtca attgtaacca ttgccaaaat ggatgaagtg
      121 tttacaatga ccaacagttt agaagaagca ttaacaattt ttaaaaatag aaaatgaaag
      181 tagatgtact tttaggatta cagtggggtg acgaaggaaa aggaaaaatc gttgacgtat
      241 taacacctca atacgatgtt atcgcacgtt ttcaaggtgg accaaatgct ggacatacct
      301 tggagtttga aggaataaaa cacgtattgc ataccattcc atctggaatt tttagaaaag
      361 aagtggtaaa cgtggtgggg aatggcgttg ttattgatcc agtgattttt aatggcgaat
      421 tggaaaagtt aaaaccattc aacattgatt acaaaaaaac gttgttgctt tcaaaaagag
      481 cacatttaat tttgccaact caccgtttgt tagaccaagc gtcggagcaa gcaaaaggaa
      541 aaaataaaat tggttcaact ttgaaaggta tcggaccaac gtatatggac aaaacaggta
      601 gaaatggttt gcgagtaggc gatatcattg ctcctaattt taaagaaaaa tacgatgcgt
      661 tggtggaaaa acacttgggc attttaaaac attacgaaga ttttactttt gatttagcaa
      721 ctttagaaaa accttggttt gaaggaattg aagtgttaaa acaattggaa attattgata
      781 gtgaacacta tatcaacaac gcgttgaaag ctggtaaaaa agtattggca gaaggtgctc
      841 aaggtactat gttggacgta gattttggaa cttatccgtt tgtaacatct tctaacacgg
      901 taactgcagg aacatgtaca ggtttaggtg ttgcacctac taaaattggt agtgtaatcg
      961 gaatttttaa agcatattgt acacgtgttg gttctggacc atttccaact gaattaaacg
     1021 acgaagttgg tgaaaaaatc cgtaaagatg gacacgagtt tggtgctaca acaggtcgtc
     1081 caagaagaac tggttggatt gacttaccag cattgaaata tgcaattatg atcaacggag
     1141 caaccgaatt gtcaatgatg aaagcagatg ttttggacga ttttgaaacc atcaaagtgt
     1201 gtacacatta catcatcaat ggagaaaaag tggatttctt cccgtttgat atcaatgatg
     1261 tggaagttac tccagtttat gaagaagtga aaggttggaa ttgcagctta ataaaattga
     1321 ctaattttgc agatgcgcca actgaattga aagattatgt aaaatatttg gaacaaaaat
     1381 tggaagtgcc aattactgtg gtttcagttg gaccagatag aacgcaaact ttgaatacaa
     1441 aaaattgatt tgtaaagtca acatagttag aaaatttgcg ctttttgtaa gcgcattttt
     1501 tttgtgtttt cacgtttttt cacaagcgtt tggcgaatta ttgcctggat ctagcgcttt
     1561 aatatacgac gaatccaaag gaattcataa attattggat ggtgccaact ttgtttatca
     1621 aggaaacact atgtattgcg attctgccta ttatattgaa aaagataaaa cagttcgcgc
     1681 ctacggtcat gtcaatattc gcaagtcgga taacgtgagt ttgttttgcg attcgttgtg
     1741 gtaccattcc tctaacgggt tggcctattt atggggacac gtgcgggcaa atgatgcgca
     1801 ataccgcctt acgaccgatt ccatggtcta taacacaaaa acagaacaag gaatttaccg
     1861 caatggtgga aaaattgaaa acatgttgca atacgaagta ttgaccagta aaatcggcta
     1921 ttttcaaccc aattctaaaa actttttctt tcgccagaat gtggtttaca aatccgatca
     1981 attgcgcatg acaacggatt cattgcaata caaatacgaa aagaaaacat gttattttta
     2041 tggtccaacg tgtattgtta ccgacgatgc ttttttagaa ggtgaaaaag gttggtacaa
     2101 tgtggacacc gaagaaggaa gtattgctaa aaatgcagtg atagagcaac ccaatcgcat
     2161 tattaaagga gattcgcttt attattcacc aaaaacaaaa attgccgaag gatttaacaa
     2221 cgtttcggtg ctggataccg ttgataaaat agcttttcag agcgattatg cattaatgaa
     2281 cgattcattg cattactcac ttcttaccaa aaatgcgatt ttaaccaaat ttacccaaaa
     2341 agatacgctg cacattcacg ctgatacact gtacaatttg aatgattcgc tcaataaacg
     2401 tgtgcaaacc ttaggctatc acaatgtgcg tatttttagt tcaaaagtgc aaggaattgg
     2461 cgatagttta tcgtttacca aaaaagatgg ctacatggaa ttgtataaaa aacccatgtt
     2521 gtggtccaaa aatggagagc taaaaggcga ttcaatacgc gtgtacatga acgattcaat
     2581 tgtggataga gcaagaattt ggggcaaggc aactgcggta atggagttgg attctggaaa
     2641 ttattacaac caattgggag gaaaagaaat tttggcgcat tttgaaaaaa atgaagtaaa
     2701 aaccgttaaa gtaatgggga atgcacaaac cgtatttttc cctgaagaag aaaaaggatt
     2761 agaaaatgtt ccgctagaag acagtcttgt atccttaaaa gatacgagtg atttttccaa
     2821 tcttgcatta caatcagact cattagcggt ggacagtgtg ccgatgaaac aagtttttgt
     2881 aatcaaacga atgggaatga accgttttta tgcttccgaa gttaaagtat atttagacag
     2941 tggagaagtg gtgggcgtaa cctattacga aaaacccgat ggtgtatttt atcccatgga
     3001 tcaaatcaat aaagaagagc aatttattca aaacttttct actaactttg ctttacgacc
     3061 aaagtcgttt gcagatttgt taaaataatt catcacccta caaaaataaa gttcatgcaa
     3121 aattttgaag catacaatcc aacacgcatt gtttttggta aaaaccaata ccaccgaatt
     3181 gcagaattag cggcagaaaa taaggcaaca aaaatcttag ttgtttatgg aggtggaagt
     3241 ataaagaaaa atggtgtgta tgagcaaatt atgcagcatt tgcacgcatt tcaggtagtt
     3301 gagtttagtg gagtaggagc caatccagaa tacaccacct tgatgcaagc cgtagaattg
     3361 gcacgcaaag aaaaaataga ttttatttta gctgtaggcg gaggttcggt aattgacggg
     3421 gtaaagttta ttgcagcagc catccattat tcgggcaatg cttgggatgt attgatgaaa
     3481 aagccaggat gtaaatttgg tgctgctgtt ccatttggag tggtgcttac gcttccagca
     3541 acaggttcag aagccaattc gggtgctgtt atcaatcgct cagaaatgaa agaaaaacgt
     3601 acaatgggcg gccccaattt attccctcaa ttttcaattt gcaaccccgg agtagttgct
     3661 tcgttgccac aacgtcaaat tgcgaatgga atagttgatt cgtttatgca tacattggaa
     3721 caatatttaa cctatccaag tggaaatttg ttgcaacaac gccatgccga aggaattttg
     3781 caaacattga ttgaaattgc gccaattgtt ctacaaaatc caagcaacta tgaagccgca
     3841 gctaatttga tgtggtgttc aacacatgcg ctcaacggaa atctacgtgc tggcgtgcct
     3901 acggattggt gcacccacat gataggacat gaattgaccg ctttttatgg cattgatcat
     3961 gcacaaacat tggcaattat tgcacctcgc ttgtttgaaa accaatttga aaataagttg
     4021 gaaatgttgg tgcaatatgg taaacgtgtg tggaatttgg aaggaacagc cacagaaatt
     4081 gcgcaacaag ccattgtgaa aacagaaaac ttttttcaac aggttggcat caaaacacgc
     4141 atttcagact atacaaccga tacttctttt attgctcaag aaattaaaaa tcgtttcatt
     4201 gaaagagggt ggacagaaat gggagagcgt aaagccatca caccaaatga tgtgtatgaa
     4261 atagttgaaa aatctatata aatagtgtgt gtttagaacg ttcaatggaa ttaaattaga
     4321 ttgttgattt cttttttgtg ctattttttt aaaaatgttt acttttgata gcttttaaaa
     4381 ataatatcaa ttgtcagacg cagtagtaat cataccaacc tttaacgaaa aggaaaatgt
     4441 cgaaagaatg acacgtaaag tcatgtcttt ggacaaagaa tttgatatat tatttgtgga
     4501 cgatgcatct ccagatggaa cagcatctat tataaagcaa ttaatgactg aatttcctaa
     4561 ccgtgttttt attgaagaaa gaacaggtaa acttggtttg ggaacagctt acattcatgg
     4621 ttttaaatgg gcaattgcaa aaaaatacga ttatattttt gaaatggatt gcgatttctc
     4681 gcacaatccc gatgatttaa ttcgtcttta taatgcttgt gcaattgatg gcgcagattt
     4741 tagcataggt tcacgttacg tagcgggcgg aaaagtggaa aactggcctt taaaacgctt
     4801 gttgatgtcc tattttgcta gtgtttatgt gcgtttaatc ttgttcatca atattaaaga
     4861 caccacagca ggatttaagt gttattccag aaaagtgttg gaagccattc atttagatgg
     4921 aaatacattt aaaggttatg catttcaaat tgctatgaaa tatgcggccc taaaacatgg
     4981 ttttaaagtt aaagaagtgc caattacatt catagatcgt gtttatggaa catctaaaat
     5041 gagtacaggt atttttaaag aagcattttt tggggtgtgg aaaatgagaa gaatgaaatt
     5101 atgaaaaatt atttgataaa aaatgcctct attgtcaacg agggtcaaac tataaatggt
     5161 gatgttttca ttaaaaatgg catcatcgaa aaagtgggga gcaatttaac ggtaaccgaa
     5221 aatcatgaag tcattgatat taatggcgct tatttgttgc ctggttgcat tgacgatcaa
     5281 gtgcatttta gagaaccggg attaacccat aaaggaacaa tagcttcaga atctagagct
     5341 gcagttgcag ggggaattac atcgtttatg gaaatgccta atacagttcc taatgcgttg
     5401 acacaagaac tattggaaga taaatacgcc attgcttcca aaacatctat tgccaattac
     5461 tcctttttta tgggagcatc caacgaaaat ttggatgaag cattaaaaac caatccaaaa
     5521 aatgtatgcg gattgaaagt gtttatgggt tcatccactg gaaatatgtt ggtggataat
     5581 gaaaaaacat tggataatat tttctccaaa ataccttttc ttattgcaac ccattgtgaa
     5641 gacgaatcaa ccgttcgtgc caataaagaa cactaccaag caatttatgg cgatgaaata
     5701 ccggtagaat tgcatcccat cattcgttcc gaagaggctt gttatttatc atcgtcactt
     5761 gcggtgagtt tagcaaaaaa acatgggtcg cgtttgcata ttttgcacat ttcaacagca
     5821 aaagaattgg atttgtttga taattccatt ccgttggagc agaaaaaaat taccgccgaa
     5881 gcatgtgttc atcacttgtg gtttagcgat gaggactata aaacaaaagg aaatttcatc
     5941 aaatggaatc ctgctgtaaa aaaagcaagc gatagagcag ctatttggca agcagtttta
     6001 gacaatcgca ttgatgtcat tgcaaccgat catgcgccac acacaatcga agaaaaaatg
     6061 ttgccttctt attttcaatc gccttctggt ggaccattgg tgcaacatgc attggtggca
     6121 atgttggaaa aagtgcaaca aggtgttttg tcgattgaaa aagtggttga aaaaatggca
     6181 catgcgcccg caattttatt tcaaatcaac aaaagaggtt ttattcgcga aggctatcac
     6241 gctgatttag ttgtggttga aaaacaacca aatgtagtgg ataaaccatc tattttgtac
     6301 cactgtggtt ggtcgccatt tgaaggagtt acattctctc acaaagtaac caaaacattt
     6361 gtaaacggta atttggtgtg gaataacaac cagcttgtag aaggaagcct tggaagcaga
     6421 atgctttttg acagatgatg cgttggagct acatattttt gttaacactg gggctatttg
     6481 cttgcaaacc aacagttgaa gtgggggcgg aaaaacctgc aactttgttg accaaagagc
     6541 aaatggtggc cattttaacc gatttaacca ttatggaatc ggtgtaccaa caaaaataca
     6601 tccaagtgcc gcgttttagt tatttgatga ttaaagaggc cgattcaatt tttaaaaagc
     6661 atggcaccag tcagttggta tttgatgcca atatggatta ttacagtgct gatttagaag
     6721 ccttagaatc catttatcaa caagtgaaaa gtaatttaat tgcccaacaa aacgcattgc
     6781 caaaagtgga gacacttcct caagatgata gctccaaagt aaataaaaaa gctttgaatg
     6841 gattgccctt tcatacagaa gaaagcatgc agcaccaaga agcaaataaa aacaagtaat
     6901 aacaacaatt tataacgata gaacatgact gtttggataa ttttctttgc attaattacc
     6961 ttttttttag cacttgattt aggagtgttt cataaaaaag atgctgtagt aaccattaaa
     7021 gaatcgttga tttggacagc tatttgggtg gcggtggcct tactttttgg tattgcaatt
     7081 tactttattt acgaaaatca attgtttgga ctcaacacgc acggtataaa tggaatagat
     7141 gctatgacgg agtactacac aggttatgtg cttgaagaat cgttgagtat ggacaatatt
     7201 tttgtgattg ccatgatttt taaatttttt aaaattcaac ctaaatttca gcaccgtgta
     7261 ttgttttggg gaattattgg tgcggttgtt ttccgcttga ttatgatttt gttaggacaa
     7321 gctttcatcg agcaattctc ttggtcgatg tatgtgtttg gtggaatttt aatctggtcg
     7381 gcgtataaaa tgttgaaaga cgaagacgaa gaaagcgatt ttaaagaatc gttgggagtg
     7441 cgtattttga gtaaaatcta tcctattaat tggaaaattc aaaacggaca ttttattgaa
     7501 aaaattgatg gaaaaacagt ggcaactcct ttgttggcaa cattaattgt aattgaattt
     7561 accgacattt tgtttgctgt agattccata ccagctattt ttagtatcac aaccgatccg
     7621 ttcattattt tcagttccaa catttttgcc attcttgggc ttagaagttt atactttttc
     7681 ctttctggaa tgttggataa atttgagcac atcaaaacag cgttggtttt tgtgttgatg
     7741 tttgtaggat tgaaaatgat tttccacgat tacctccatt tcttgagtga acatacgtgg
     7801 atttcattag ttgtaatttt aacattcttg agcggaggag ttattgcttc catcatttca
     7861 aacaagcgca aagagaaaga aaatttgggg taaatcacct gtgtagaaat tgttattaat
     7921 ttggtgataa attattgcat ttttctcttg caaattgatt ttgattttca ttatctttgt
     7981 tcctgatgga aaagcaaatc attttgaata aaaggcatat cgagttgacg ctaaaacgcc
     8041 tttgttatca attgattgaa tctcacaacg atttttcaaa tgctataatt attgggttgc
     8101 agccacgtgg aattcacgtt gtaactcgtc ttaaaaaaga attggaagcc attcttaaca
     8161 aagaaatagt gtgtggtaat ttggatatta cgttttatcg ggatgatttc agaagaagag
     8221 aacgaccgtt gataccaagt gtcaccaaca tcgattttga tattgaagga aaaaacatca
     8281 ttttagtgga tgatgtgctc tataccggtc gaacaattcg tgcaggcctc gatgctttgt
     8341 tggcatttgg acgcccgtca aaagtagaac ttttagcatt tatagataga agatttaacc
     8401 gtgatttgcc gatacaagca gattacatag gagttaatgt agatacactt agccaagaac
     8461 gtgtttcggt tgagtggaat gaaatagaag gagaagataa agtaatctta tttacacctg
     8521 aaaacaatgg ataatttaag cgttgatcat ataattggaa ttaaagactt gacaaaagca
     8581 gatattgaat tgattttaaa aactgccgaa acttttaaac aagtaattaa tcgaccaatc
     8641 aaaaaagttc cttcacttag agatattacc attgccaatc tgttttttga aaattctacc
     8701 cgcacacgct tgtcgtttga attagccgaa aaacgcttgt ctgccgatgt agttaacttt
     8761 agtgcagcca atagttcggt gaaaaaagga gaaacattgg tggatacaac caataacatc
     8821 ctttcaatga aggtggacat ggtggtaatg cgccatccta atcctggagc tgccaaattc
     8881 ttgtcagaac gtgtcaatgc acgcatcatc aacgcaggag atggaacaca cgaacaccca
     8941 acacaggcct tgttggatgc tttttcaatt cgtgaacaat tgggaacatt ggaaggtaaa
     9001 aaagtggtga ttgtggggga tattcttcac tcacgcgtgg cactttccaa tatttattgc
     9061 ttgcaaaaaa tgggcgctga agtaatggtg tgcggaccaa ctaccttgat tccaaaatac
     9121 atctcagaat tgggtgtgaa agtagagcac aacttacgca aagccttgga atggtgcgat
     9181 gctgccaata tgttgcgcat tcagttagaa cgtcaagata tcaaatattt cccttcattg
     9241 cgtgagtaca gcatgctctt tggattggat aaaaaactgt tggattcatt ggataaaaag
     9301 attgtgatta tgcacccagg accaatcaat cgtggggtgg aaatcaccag cgatgtagcc
     9361 gattctgacc aatccatcat tttgcaacaa gtagaaaatg gagtagccgt aagaatggct
     9421 tgcatctatc tgttggcttc taaaattgag cgttaatgca cgaatggtcg gaagaaattt
     9481 cttccgactt ttttgtattt attaaccttt aa
//
LOCUS       DAOQVQ010000037         9503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8050_length_9503_cov_4.760584, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000037 DAOQVQ010000000
VERSION     DAOQVQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8050_length_9503_cov_4.760584"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PKN22_02130"
     CDS             <1..1215
                     /locus_tag="PKN22_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNS41530.1"
                     /translation="IKDIVSASSPILVQVAKEPISSKGPRLSAEITLAGRYLVLVPFS
                     DKVSISQKIRDAKERDRLRDLVEAIRPKNFGVIIRTVAENKKVEQVEQDLKDLLNKWK
                     TMHSRLKNAVPPMRVLDEMGKTTSILRDMLNADFTNIHTNNSTTYDELKEYISGIAPE
                     REKILKLYDGKLDIFEKFGINKQIKTLFGKKVPLPSGGYLIIEHTEAMHVIDVNSGNR
                     KGADGQEANALLTNMEAAEEIARVLQLRDMGGIVCVDFIDMHDKANNKALYDHLKEVM
                     KSDRAKHNIIPPSKFGVVEITRQRVRPETEIKTAETCPTCNGTGEIQASILFAEEIEN
                     NLQFLVLDRKEKHVALLVHPYLEAYFKKGLISRQVKWFFKFKKWIPVRRATANQLLQY
                     SFVDNNNEEIAL"
     gene            1244..1690
                     /locus_tag="PKN22_02135"
     CDS             1244..1690
                     /locus_tag="PKN22_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNS41531.1"
                     /translation="MTAKQKIEAFCAYQDRCHFEVSNKLNQWGIVGEQKDQLMAHLIE
                     QRFLDEQRFADSFVSGKFRIKRWGKIKIRQQLKAKFVPLTCINKALQQIDGDDYFATL
                     VALATKKHKELSNEKDSYTKKAKIMRFLASKGYEQDLIYDAVESLT"
     gene            complement(1696..2727)
                     /gene="porQ"
                     /locus_tag="PKN22_02140"
     CDS             complement(1696..2727)
                     /gene="porQ"
                     /locus_tag="PKN22_02140"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNS41532.1"
                     /translation="MKKILLFVLLLIAGNAFSQMGGKNAYALLGLTFNARSAGLAGNF
                     ITAKDYDINLGVANPSLLNEQMHQVVSVSQALYAGRINYGMFSYGHQIKEKHTIAAHI
                     RYVAYGKMTRTDKNGVEMGTFSPFEYILGVGYAYKINPLFSVGANVNIIGSHLEVYNS
                     YGTSIDLAATFLNKSETFLATVLFKNVGFQFNKYGTKRAPLPADFQLGVSYRLKHAPF
                     RFSIVYHHLNKWDLTYSDPNAQPTVDLLTGDTIPVKKAKFFEKLGQHFTYQIEILATK
                     NIHFRAGFDYYTRQSMKLEKKPGIAGFSFGLGLHFRKISFDYGFSFYSKAGFNNMITL
                     SSNIGAFRK"
     gene            complement(2766..3263)
                     /locus_tag="PKN22_02145"
     CDS             complement(2766..3263)
                     /locus_tag="PKN22_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNS41533.1"
                     /translation="MVKIGLLSDTHGDLDERIFHYFKDVDEIWHAGDIGTLDVLDKLK
                     AFKPVRAVYGNIDDHTIRLEAPLHNRFTIEKVEVLMTHIAGKPGKYAKPAYDELQKGA
                     PKLFVCGHSHILLVKFDPHYNMLWMNPGACGNKGFHQIKTLLRFCISDDRIHDLEVIE
                     LEKRV"
     gene            3306..4358
                     /gene="meaB"
                     /locus_tag="PKN22_02150"
     CDS             3306..4358
                     /gene="meaB"
                     /locus_tag="PKN22_02150"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNS41534.1"
                     /translation="MDKQQMHEDWLRKRKQRKAELTVDALLDGILQHNTTLLSSAITL
                     IESTLESDRVKSEELIKRCLPHCGKSIRIGITGVPGVGKSTFIESFGQLLVQAGKKVA
                     ILAIDPSSSTSGGSILGDKTRMEQLSQLHNVFIRPSAAGDTLGGVARKTRESIFLCEA
                     AGYDVILIETVGVGQSETLVHSMVDFFLLLMLSGAGDELQGIKRGIMEMADALVITKA
                     DGDNVKKANLARREYQNAMHLFPPKKSNWQPKALTCSSYNNTNMDMIWETILAFENQT
                     KINGYFAHNRKEQDKFWLRETLNESILRGFYGNEHLHELLKEMDKKVINEEETSFTAA
                     EKLYQLYIQHLSNGNI"
     gene            4345..4551
                     /locus_tag="PKN22_02155"
     CDS             4345..4551
                     /locus_tag="PKN22_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNS41535.1"
                     /translation="METFKIEGDYIELISLLKVLGIAQTGGQAKFIVDEGVVIRNGEQ
                     EFRKRAKLIAGDVIEIGEIKIQLL"
     gene            complement(4543..5079)
                     /locus_tag="PKN22_02160"
     CDS             complement(4543..5079)
                     /locus_tag="PKN22_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41536.1"
                     /translation="MKQLVVFAVIVLLISACGGKKNELSYDQRIAQYEDSIEQWGGGL
                     GSQEEINGFAERYINLLLETYNEESSNPNNATYLDRVHMWYASIGKPNESVKWGETLL
                     KKFPNYSNRVMVIESMAAMYDGDITPRDSNKVRSYYTQLLTEFPKLNPEKRKDIEKRL
                     KYNNLSLEEYIVTVSVSE"
     gene            complement(5079..5672)
                     /gene="def"
                     /locus_tag="PKN22_02165"
     CDS             complement(5079..5672)
                     /gene="def"
                     /locus_tag="PKN22_02165"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNS41537.1"
                     /translation="MILPIVAYGDPVLKREADEIDENYPNLKELIDNMFETMYEASGV
                     GLAAPQVGLSIRLFVVDGSPFADEEDEEPDPRAVGMEGFKKVFINPIIEEEKGENWGF
                     HEGCLSIPKIREEVFRKEELTISYYDENWQFHEEKFSGYKARIIQHEYDHIEGILFTD
                     HLSALKKKMLTKKLQNISKGDISVDYKMKFPAQKKGR"
     gene            complement(5742..6728)
                     /locus_tag="PKN22_02170"
     CDS             complement(5742..6728)
                     /locus_tag="PKN22_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41538.1"
                     /translation="MNRMVFLVLLVFGLYLTSCNTPVLESSTPKRFYIASDFLQPKDA
                     ILFKDFSKKEKTRVVILNWTADSIEQHYAKFGYSSKFDAVLMKTTFAVNQFTTQKILH
                     PIEANYLWQNKDFEAPNKEWITLGIDPYVIAGLKEKKVMHYNDLTWGNKWENKLTPEE
                     MKAFNGAVVYQFGRKNLQNSIKWLKKLHQQIAPAQPMLVAQPDSLADSTALASFYLTR
                     LSKINPKKEVFVYPSQIVRLGAFYDGINFGIARHSSKYITALHFIAFYTQPLNNQKLS
                     KRLHVLPVENPHKQSPFDYQNNYPILFRGSPGEIAPIFRNLSKIEVRIYGGN"
     gene            6832..7191
                     /locus_tag="PKN22_02175"
     CDS             6832..7191
                     /locus_tag="PKN22_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41539.1"
                     /translation="MEDLTAIIAILSTIALPIGFGMYLGLRANNQKHVERMEMIKQGI
                     IPPDNVETDAKKYKSLRNGLLFVGMGIGFIVGLTIIKTMRLNEDAGFLVLGASFLLFF
                     GLAYLAYFKIVSNKEKE"
     gene            7191..7721
                     /locus_tag="PKN22_02180"
     CDS             7191..7721
                     /locus_tag="PKN22_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS41540.1"
                     /translation="MQVNEKQLIRQILEGETSSFSYFVDTYQDMALTLAFRICANRQD
                     AEDITQNAFVKAFHNLHTFRATSKFSSWFYRIVYHTALNFIKQNQLKTVEVDENTGVS
                     EENNATLIEKEENLNRLEVALNQLPKDEVTIVTLYYLDEKPIKEIAEVVGLTESNVKI
                     KLHRSRQKLKEYLTVE"
     gene            7723..8046
                     /locus_tag="PKN22_02185"
     CDS             7723..8046
                     /locus_tag="PKN22_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41541.1"
                     /translation="MDEQDLKKQLLATQLKANDGLKSRIMHQINAEQSLLPQQSKATS
                     LLKEKGVFIFSGLYVFIALIGGYFYISTKENPLLSPSFLTICCVVASIFSVYWLFTAL
                     KYAKK"
     gene            8103..8666
                     /locus_tag="PKN22_02190"
     CDS             8103..8666
                     /locus_tag="PKN22_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41542.1"
                     /translation="MKRNFLVILSLLFLVACSDLKKGEQLKKVQELQTSLDSIKKSWN
                     EADIKKIDSISAICHTTIDSISLLYDDRYVPMSLAIKLDQFKQCNQGMDELKRIHAFF
                     PTLLVDKMQALEKLKKDIQEGKGRPEKYDEYLDFEKNELITIDKQYKRYLFTKKKSFD
                     NYSKSNEAVCQFLDELKIALAQSHTSN"
     gene            8727..>9503
                     /locus_tag="PKN22_02195"
     CDS             8727..>9503
                     /locus_tag="PKN22_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41543.1"
                     /translation="MVVVFTLFGGTFSCYAQPATDVQLADYYYNNGELDKAKAYYEKL
                     YKKNPIKTYFDRYYACLLNENDQKSIEKLLKQQIENSRGEAEYSVMLGQFYEENQQND
                     KAEKIYKTLVAELSPNPTAVINLYNAFKAKGKNEWAFQTITKGRSMLKDAYPLNFQFA
                     EYYGANKETKKMVQEYLNLIDFNSGYTETVQTILSRVIDFNNKSSEEYAILKTELLTR
                     AQKNPNDVRAAEMVTWLFIQSRDFPAALIQEQALDKRLQLN"
BASE COUNT         3152 a         1691 c         1791 g         2869 t
ORIGIN      
        1 attaaggata tcgtatctgc ctcatcgcct attttggtac aagtggcaaa agagccaatt
       61 tcaagtaaag gacctcgttt aagtgctgaa ataacacttg cgggtcgtta tcttgttttg
      121 gttccttttt cggataaagt ttcaatttca caaaaaatta gagacgcaaa agaaagagac
      181 cgcctgcgcg atttagtaga agccattcga ccaaagaatt ttggagtcat catccgtaca
      241 gttgccgaga acaaaaaagt cgagcaagtt gaacaagatt taaaagattt gctgaacaag
      301 tggaaaacca tgcattcacg cttgaaaaat gctgttccgc ctatgcgtgt attagatgaa
      361 atgggtaaaa ccacttctat attgcgcgat atgctcaatg cagatttcac caatattcac
      421 accaacaact caacaaccta tgacgagttg aaagaataca tcagtggaat tgcaccagag
      481 cgtgaaaaaa tattgaaatt gtatgatggc aaactcgaca tttttgagaa gtttggcatc
      541 aacaaacaaa ttaagacatt atttggtaaa aaagtgccgc ttccaagtgg aggttacttg
      601 attatcgagc atacagaagc gatgcacgta attgatgtta actctggaaa cagaaaaggt
      661 gctgatggcc aagaagcaaa cgctttgctt accaatatgg aggctgcaga agaaattgcg
      721 cgtgtgcttc aattgcgaga tatgggcgga attgtgtgtg ttgattttat agatatgcac
      781 gataaagcca ataacaaagc actatacgac cacttgaaag aagtgatgaa gtcggataga
      841 gcaaaacaca atattattcc tccatctaaa tttggagtgg tggaaattac acgtcaacgt
      901 gtgcgaccag aaacggaaat taaaacagcc gaaacttgtc ctacatgtaa cggaacaggt
      961 gaaattcaag caagtattct ttttgccgaa gaaattgaaa acaatttaca gtttttggtg
     1021 ctcgatagaa aagaaaaaca tgttgctttg cttgttcacc cttatttaga ggcttatttc
     1081 aaaaaaggat tgatatcccg tcaagtgaaa tggttcttta aatttaaaaa atggattcct
     1141 gtcagaagag ctactgccaa ccaattgttg caatactctt ttgtggataa caataacgaa
     1201 gaaattgcac tttgacatga agtcggagtg taatttctct tttttgacag cgaagcaaaa
     1261 aattgaagct ttttgtgcct accaagatcg ttgtcatttt gaagtttcca ataaattaaa
     1321 tcaatgggga atagttggtg aacaaaaaga ccaattaatg gcccatctca ttgaacaacg
     1381 ttttttagat gaacaacgtt ttgctgatag ttttgtgtcg ggtaaattta gaattaaacg
     1441 ttggggaaaa attaaaattc ggcaacaact caaagctaaa tttgttccgt taacctgcat
     1501 taacaaagct ttgcaacaaa ttgatggcga tgattatttt gctacacttg tggcattagc
     1561 aacaaaaaaa cacaaggaac tttctaacga aaaagacagt tataccaaaa aggctaaaat
     1621 catgcgtttt ttagcatcaa aaggctatga gcaagattta atttatgatg ctgttgaatc
     1681 actcacctaa ttgccctatt tcctaaacgc accaatgtta gatgacagcg taatcatatt
     1741 gttgaaacca gcttttgaat agaaactaaa accataatca aacgatattt ttctgaaatg
     1801 caagcccaaa ccaaacgaga atccggcaat tcctggtttt ttctccagct tcatagattg
     1861 acgggtgtag taatcaaatc ctgcacgaaa atgaatgttt ttggtagcta aaatttctat
     1921 ttgataggta aaatgttgcc ctaatttttc aaaaaacttg gcttttttaa ctggaatggt
     1981 atcgcctgtt aatagatcta ctgttggttg tgcattcggg tcagaatagg ttaaatccca
     2041 tttgttaaga tgatgataaa caatagaaaa tctgaacggc gcatgtttta aacgatagct
     2101 aacccctaat tgaaaatctg caggcaaagg tgctcgttta gttccgtatt tattaaactg
     2161 aaaacctacg tttttgaaaa gcacagtagc caaaaatgtt tcagatttat ttaagaatgt
     2221 tgctgccaaa tcaatggatg ttccgtagga attatacacc tctaaatgcg atccaataat
     2281 gtttacattt gctccaacag aaaacagtgg gttaatttta taagcataac caacgccgag
     2341 gatgtattca aacggactaa aagttcccat ttccactcca tttttatcgg ttcgggtcat
     2401 ttttccataa gcaacatagc ggatgtgtgc tgcaatggtg tgtttttctt ttatttggtg
     2461 tccgtaacta aacattccat agttgatgcg cccagcatac aacgcttggc taacacttac
     2521 aacttggtgc atttgttcgt tgagcaatga aggattggca acccctaaat tgatgtcgta
     2581 atctttggcg gtgatgaaat ttcctgcaag tccagcacta cgcgcattga aagttagccc
     2641 caacaaagca taagcatttt ttccacccat ttgtgaaaat gcatttcctg caataagtag
     2701 taaaacaaat aaaagtattt ttttcatcct catttcaaac gcaaagaaac aataaaaatt
     2761 atatcctaca ctcttttttc aagttcaatt acttccaaat catgaattcg gtcatcggaa
     2821 atgcaaaaac gcaacagggt ttttatttga tgaaaccctt tgtttccaca agctccaggg
     2881 ttcatccaca acatgttgta gtgcggatca aatttaacca gtaaaatgtg cgaatgtccg
     2941 caaacaaaca actttggagc tcctttttgc aattcatcat aagctggttt ggcatatttt
     3001 ccaggtttac cggcaatgtg agtcatcagc acttcaactt tttcaattgt aaagcgattg
     3061 tgcaaaggtg cctccaagcg aatggtgtga tcatcaatgt tgccatatac ggctcgaaca
     3121 ggtttaaatg ccttgagttt atcgagtaca tccaatgttc caatatcgcc cgcatgccat
     3181 atttcatcta cgtctttgaa ataatgaaaa atacgctcgt ctaaatcgcc atgtgtgtct
     3241 gaaagcaaac caatttttac cataaaacaa atttaatgag atatttcgat tatttttgtt
     3301 ttgctatgga taagcaacaa atgcatgaag attggttgag aaaacgcaaa caacgcaaag
     3361 ctgaattaac ggttgatgcg ctgttggacg gtattcttca gcacaacaca acgctgctta
     3421 gtagcgctat cacccttatt gaaagcacct tggagagcga cagggtaaaa tcggaagaac
     3481 tcatcaaaag gtgtttgccc cattgtggaa aatccattcg aattggcata acgggcgtac
     3541 ctggtgttgg aaaaagtacc tttattgaaa gttttggtca gttgttggtg caagcgggta
     3601 aaaaagttgc cattttggcc atcgaccctt cttctagcac ttcgggaggt agtattttag
     3661 gcgataaaac aagaatggag cagttatcac aattgcacaa cgtttttatt cgtccatcgg
     3721 ctgcaggaga cactttggga ggggtagctc gcaaaacgcg agaaagcatt tttttgtgtg
     3781 aagctgctgg gtacgatgtc attttaattg aaacggtggg tgtgggacaa tccgaaactt
     3841 tggtgcattc aatggttgat tttttcttgt tgttgatgct atcgggggca ggagatgaat
     3901 tgcaaggcat caagcgtgga attatggaaa tggccgatgc gcttgtgatt accaaagcag
     3961 atggcgacaa tgtgaaaaaa gccaatttgg ctcgtagaga ataccagaat gccatgcact
     4021 tgtttccgcc aaaaaaaagc aattggcaac caaaagcact cacgtgttca agttataaca
     4081 ataccaatat ggacatgatt tgggaaacta ttttggcttt tgaaaatcaa acaaaaatca
     4141 acgggtattt tgcacacaac cgcaaggaac aagataaatt ttggttgcgc gaaacgctca
     4201 acgaaagcat attaagaggt ttttacggta atgaacattt gcatgaattg ctaaaagaaa
     4261 tggataaaaa agttattaac gaagaagaaa cttcttttac agcagcagaa aaattatacc
     4321 aattatacat tcaacattta tccaatggaa acatttaaaa tcgaaggcga ttacattgaa
     4381 ctcattagtt tattgaaggt tttaggcatt gcacaaacag gcggacaagc aaaattcatt
     4441 gtagatgaag gggttgtcat tcgaaacggc gaacaagaat ttcgcaaaag agccaaactt
     4501 attgctggag atgtaattga aattggagaa ataaaaatcc aattactctg atacagaaac
     4561 cgtaacaatg tattcttcga gcgataaatt attgtatttc aatcgctttt cgatgtcttt
     4621 tcgtttttct ggattcaatt ttgggaattc ggtcaacagt tgtgtgtaat aactgcgcac
     4681 tttgttacta tcgcgtggag tgatgtcgcc atcatacatt gccgccatgc tttcaatgac
     4741 cattacacgg ttggaatagt tgggaaattt tttcaataac gtttcgcccc acttcaccga
     4801 ttcgttgggt tttccaatgg aggcatacca catgtgcacg cggtccaaat aggttgcatt
     4861 attcggatta gatgattctt cgttgtatgt ttccaataat aaattgatgt agcgctcagc
     4921 aaaaccatta atttcttctt gtgagcctaa accgccaccc cattgttcaa tagaatcttc
     4981 gtattgtgca attcgttggt cgtatgaaag ttcatttttc ttaccaccac acgcagaaat
     5041 caacaaaact attactgcaa aaactaccaa ctgtttcatt atctaccttt tttttgtgct
     5101 ggaaatttca ttttataatc taccgaaata tcgccttttg aaatgttttg caattttttg
     5161 gtcaacattt ttttcttcaa tgcagaaagg tggtcagtaa acaaaattcc ttcaatatga
     5221 tcgtattcgt gttgaatgat tcgggctttg tatcctgaaa atttttcttc gtgaaattgc
     5281 caattctcat cgtagtagga aatggtcaac tcttcttttc taaaaacctc ttctctaatt
     5341 tttggaatcg acaaacaacc ttcgtgaaaa ccccaatttt cgcctttttc ttcttcaata
     5401 atcgggttga taaacacttt tttaaatcct tccatgccaa cagcacgtgg atctggttcc
     5461 tcgtcttcct catcggcaaa tggagagcca tccaccacaa ataatcggat ggataaaccc
     5521 acctgaggag cagccaaccc aacacccgat gcttcataca tcgtttcaaa catgttgtct
     5581 attagctcct ttaaattagg ataattttcg tctatttcgt cggcctctct ttttaaaaca
     5641 gggtcgccat aagccacaat tggtaaaatc atactttgta aaaactttgt gcaaaattac
     5701 aaaaaatagc ggttttacta gctattgaat gaaatgaaaa attaatttcc accataaata
     5761 cgcacttcta ttttgctaag attgcgaaaa ataggtgcaa tttctccggg tgagcctcta
     5821 aataagatgg ggtaattatt ttggtaatca aacggagatt gtttgtgtgg gttttcaact
     5881 ggtaatacat ggagacgttt actcaacttt tgattgttaa gcggttgcgt ataaaatgca
     5941 ataaaatgta gtgctgtaat gtattttgaa gaatggcgag ctattccaaa gttgataccg
     6001 tcataaaacg ctcccaaacg aacaatttga cttggataca caaacacctc ttttttaggg
     6061 tttattttag acaaacgagt gaggtaaaac gacgcaagtg ctgtagagtc agccaatgaa
     6121 tcgggttgag caacgagcat tggttgtgct ggtgcaattt gttggtgcag ttttttcaac
     6181 catttgatag aattttgcaa gttttttcgc ccaaattggt acaccacagc cccattaaat
     6241 gctttcattt cttcgggtgt aagtttattt tcccatttat tgccccacgt caaatcgttg
     6301 tagtgcatta cttttttttc tttgagtcct gcaatgacat agggatcaat acccaacgta
     6361 atccactctt tgttgggtgc ttcaaaatct ttgttttgcc agagataatt ggcttcaatt
     6421 ggatgtaaaa tcttttgggt agtaaactgg tttaccgcaa atgtagtttt catcaaaacc
     6481 gcatcaaatt tagaagagta tccaaatttt gcataatgtt gttcaataga atcggctgtc
     6541 caatttaaaa ttacaaccct tgttttttcc tttttgctaa aatctttgaa taaaatagca
     6601 tcttttggtt gtaaaaaatc ggaggcaatg taaaatcgtt tgggagttga agattccaaa
     6661 acaggcgtat tgcagctggt tagatatagc ccaaacacca ataaaacaag aaaaaccatc
     6721 cgattcatag cgtaaaagta attatttcta ttggtacaaa aaatgaaaaa agttgtaacc
     6781 aaatgaattt tttgttgtcc tattactgaa aaccaattaa ttttaaatgt tatggaagat
     6841 ttaacagcaa ttattgccat cctttctacc attgctttgc caataggatt tgggatgtat
     6901 ttggggcttc gtgccaacaa tcaaaaacat gttgagcgca tggaaatgat aaaacaaggc
     6961 atcattccgc ccgacaatgt agaaacggat gctaaaaaat acaaatcact tagaaatggg
     7021 ttgttgtttg taggaatggg cataggtttt atcgtgggat taacgataat aaaaacgatg
     7081 cgtctcaatg aagatgctgg ttttttggtg cttggggcta gcttcttatt gttttttgga
     7141 ttagcgtatt tagcatattt taaaatcgtt agcaataaag aaaaagaata atgcaggtca
     7201 acgaaaagca attgattcgc caaatactag agggagagac gtcttccttt agctattttg
     7261 tggacaccta tcaagatatg gcactcaccc ttgcttttcg aatttgtgcc aatagacaag
     7321 atgctgaaga tattactcaa aatgcatttg taaaagcatt tcacaacttg cacacgtttc
     7381 gggcaacaag caaattttca tcttggtttt accgcattgt ttaccatact gccttgaatt
     7441 tcatcaaaca aaatcaatta aaaacagtag aagttgatga aaatacagga gttagtgaag
     7501 agaacaatgc aacattgatt gaaaaagaag aaaatttaaa ccgattagaa gttgcattaa
     7561 atcaattgcc aaaagatgag gtaaccattg taacgttgta ttatttggat gaaaaaccca
     7621 tcaaagaaat tgccgaagtg gttggtttaa ccgaaagcaa tgtgaagata aaattgcacc
     7681 gatcgcgtca aaaattgaaa gaatatttaa ccgtagaata gtatggacga gcaagattta
     7741 aaaaaacagt tgttagccac ccaactcaaa gcaaatgacg ggttgaaatc gcgaattatg
     7801 caccaaatca atgcagaaca atccttgttg cctcaacaaa gcaaagccac atccttgtta
     7861 aaagaaaaag gagtattcat ttttagcggt ttgtatgttt ttattgcact aattggagga
     7921 tatttttaca tttcaaccaa agaaaatcca ttactctcac ctagtttttt aaccatttgt
     7981 tgtgtggtgg caagtatttt tagcgtttat tggttattta cagctcttaa atacgccaaa
     8041 aaataatttt taatctgttt aaaattccgt atttttgtac aagcacacta tttttgctgg
     8101 tcatgaaacg caatttttta gtcatcttat ccctattgtt tttagttgct tgttccgatt
     8161 taaaaaaagg agaacagcta aaaaaagtgc aagagttgca aacatcactg gatagcatta
     8221 agaaatcgtg gaatgaagcc gatattaaaa aaatagacag catttcagcc atttgtcaca
     8281 ccaccattga ttccattagt ctgctttacg acgatagata tgtgcctatg agtttagcca
     8341 tcaagttgga tcaatttaag cagtgtaatc aaggaatgga tgagttaaaa cgcatacatg
     8401 ctttttttcc tacgctgttg gttgacaaaa tgcaggcctt ggaaaaactc aaaaaagaca
     8461 tccaagaagg aaaaggacga ccagaaaaat acgacgaata tttggatttt gaaaaaaacg
     8521 aattgattac catcgacaag caatacaaac gctatttgtt taccaagaaa aaaagttttg
     8581 acaattattc caaatccaac gaagctgtat gtcaattttt agatgaatta aaaatagcat
     8641 tagcccaatc acatacttcc aattaaaatg ttttgtatct ttagcttatg attggtagta
     8701 gcatgcgtaa aatttttagt tgcataatgg tggtggtttt cacacttttt ggtgggacat
     8761 tttcatgcta tgcgcaacct gcaactgatg ttcaattagc cgattattac tacaacaatg
     8821 gtgaattaga caaggcaaaa gcatactatg aaaagctgta caaaaaaaat ccaataaaaa
     8881 cctattttga ccgctattat gcttgtttgc tcaatgaaaa cgatcaaaaa tcaattgaaa
     8941 aattacttaa acaacaaatt gaaaactcca gaggcgaagc tgaatacagt gtaatgttgg
     9001 ggcaatttta cgaagagaat caacaaaacg ataaagccga aaaaatttac aaaaccttgg
     9061 tagcagaact ttcacccaat ccaacggctg ttatcaattt atacaatgcc tttaaagcta
     9121 aagggaaaaa cgaatgggct tttcaaacca ttaccaaagg gcgaagcatg ctcaaagacg
     9181 catatccact gaattttcaa tttgcagaat actacggcgc caacaaggaa accaaaaaaa
     9241 tggtgcaaga atacctgaat ttaattgatt ttaacagtgg ttatacagaa accgttcaaa
     9301 cgatattgtc gcgtgttata gattttaaca acaaatcatc ggaagaatac gctattttaa
     9361 aaacagaatt gttgacacgt gctcaaaaaa atccaaacga tgtacgagct gcagaaatgg
     9421 taacttggtt gtttattcaa agtcgcgatt ttccagccgc attaatacaa gaacaagcac
     9481 tggataaacg tttacaacta aat
//
LOCUS       DAOQVQ010000038         9487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8067_length_9487_cov_3.612595, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000038 DAOQVQ010000000
VERSION     DAOQVQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_9487_cov_3.612595"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="PKN22_02200"
     CDS             <1..100
                     /locus_tag="PKN22_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HNS41544.1"
                     /translation="IAAHGPFVMNTYEELVEAFNDFNQGKFGSLEK"
     gene            complement(175..1917)
                     /locus_tag="PKN22_02205"
     CDS             complement(175..1917)
                     /locus_tag="PKN22_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41545.1"
                     /translation="MKQIYLTLVVGCFAFGTMAQSIQKQKEITVVKTVKTPVQNQNKV
                     DLWTNDFSTASDWAFQDLAGSGDNWVIGTTGPAGTFAIAPIASTSAANGFAKFDSDLL
                     CSGDQNALVKNVNPIDLTGEPNVTLEFESYYREFQGSAYVGFSTDGTTWTYVAVHTDL
                     GANDACANPTTVSVPAPVIGGSATAYICFRYQGGCDYAWMVDDVKIVRTPNYDLKVTF
                     QNWGSEGYQYSQIPLTQVQPTDFFVGVTNNGINNLTNIGLTVNVTGQETTSVSATPVA
                     TSVSGQIDTLVATYTPAAVGTYNFTQALSLTETDENPANNTGLPNRSFSVTNYTYAVD
                     KGTPFSTYPDLEDIFLDATIPYTEIGNSFDIVTAQDLYGIDVYIDTATANGSQFYGSL
                     YQYNTSATSGSDLWLNVDDTNPLTVTASTPKASIVSLLFSTPQSLTPGTYMISVRVLD
                     EGVVFGGSGATSGSTQSWGWIIGNSGTGAWTGLSLVPVIRMNFDPSLSVKTVDNLEGV
                     KVYPNPTKGLVNISNDNNLDNTITVTDINGKVIATKSSSAATTIDLNASNAGIYFVEV
                     VNQNGKKVEKIIVE"
     gene            complement(2028..2459)
                     /locus_tag="PKN22_02210"
     CDS             complement(2028..2459)
                     /locus_tag="PKN22_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41546.1"
                     /translation="MSSNSLYMDFYDLEDFTIGRRKDNIIVIYVKEGKEVNQEMQYAI
                     FKYLLQLKQPGVLHPVILNGGDFVSISSNVVEHTKIGFKEHVLCVAVVAKNMADRIVG
                     KYFSKKYKTSSEFVFFDSLDEAVSFCYENMNKVGMNFTPLL"
     gene            complement(2669..3952)
                     /gene="speE"
                     /locus_tag="PKN22_02215"
     CDS             complement(2669..3952)
                     /gene="speE"
                     /locus_tag="PKN22_02215"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNS41547.1"
                     /translation="MKNVALGNHILVEFMQCDPHIMNDVATIERNMVEAAEKAEATVI
                     NSTFHHFSPYGVSGVVVIQESHLAIHTWPEYGYAAVDLFTCGEMDAWIAFDYLKDCFK
                     SKSYSAIEMKRGSVNLLTRNDFDVRSMREQASGWRNPEFYTRNVWFTDKDEDQALSLR
                     FTGDVFYDVQSPFQRVRILESTKYGKMLTLDDMVMTTINDEFHYHEMISHPAMFTHGN
                     AKNVLVIGGGDGGTVREILRHEGVEKVTMVEIDGEVIKACKEHLPQIAASFDHPKLDL
                     IVNDGIAFVKNAAPKSYDIIIVDGSDPVGPAEGLFSVDFYTNCYNALKDGGILVTQGE
                     SPKFNEKAFAELNRTLQSIFGKENAPISLFFVPTYPTGMWSFQYGLKGCAAPKSISNE
                     TEIDAFVEQKGLRYYNADVHKGSFATPNFVKSLVK"
     gene            complement(4017..4658)
                     /locus_tag="PKN22_02220"
     CDS             complement(4017..4658)
                     /locus_tag="PKN22_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNS41548.1"
                     /translation="MHNFLKETQFLDFSHPLFDVFVEKIDATVSDKEKAIQLYYLVRD
                     GFLYDPHHLDLRHHALKGSTILTKKRAWCVEKGIVLAAAARKLGIPSKLGYAIVVNHI
                     GSDRLEKYLLKKEIVFHGYVSLYVDEKWVKCTPAFDKNVCRINKVTPLEWDGVQDSMF
                     QEFEGQNQFMEYIHFYGEFDDVPIELMNQEMEKHYPHLFKNRIETKEFSFYHL"
     gene            4754..5269
                     /locus_tag="PKN22_02225"
     CDS             4754..5269
                     /locus_tag="PKN22_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41549.1"
                     /translation="MKQLKVGIFGSEKPSIFTQILFYYGTLISFIFGLWSVISLAILK
                     FPNYIKQHKNVDVEAIIELRGRELGFINESFYANLENFHLYSCFIWLICFTCFFLLWR
                     KIYWASYVIVGSILVYYTLMIVILGSTYYVEDTTIFDKTTLLLLFVATIVHALIYRTP
                     EKSTNPAKTEN"
     gene            complement(5270..6241)
                     /locus_tag="PKN22_02230"
     CDS             complement(5270..6241)
                     /locus_tag="PKN22_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNS41550.1"
                     /translation="MKRILFIQTAFLGDVILATAAFSEAKRLFPEAEIDVVVKKGNES
                     LLENNPQLTHVFTFDKKAGKFKEIFRLRKVFKKRQYDIVFNFQRFGSSGILTLLSGAK
                     KMYGFKKNPFSFGFSKSFEHTIGDGTHEVERNMQLLLEFGAVKKVRPVLYPSEKQFDK
                     VAMYKQKTYYCLAPASVWFTKQLPEKKWVELIHTLGQEASIYLLGAPSDGELCERIIA
                     ASNQSNVVNLSGKLSLLESAALMKDAQRNYVNDSGPLHIASAMNAPVTAFFLSTIPAF
                     GFGPLSDDSQILDVGELYCRPCGLHGFKACPEGHFKCSEITLSAVKK"
     gene            6502..6963
                     /gene="mraZ"
                     /locus_tag="PKN22_02235"
     CDS             6502..6963
                     /gene="mraZ"
                     /locus_tag="PKN22_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNS41551.1"
                     /translation="MAGLVGEYEVKLDAKGRFLFPAGLRKQLPPVSQNDFMLNKGFED
                     CLTLYPLVEWEKISEKLSKMNLFKPKNRMFYRLFHQGAKQVALDNAGRILIPSTHVER
                     VGIKQDVMLIAYNDRIEIWDKAKYYALIEENMVDFADLADEVMGDLNDDTE"
     gene            6947..7873
                     /gene="rsmH"
                     /locus_tag="PKN22_02240"
     CDS             6947..7873
                     /gene="rsmH"
                     /locus_tag="PKN22_02240"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNS41552.1"
                     /translation="MTTQNNTYHVPVMLSECLEGLNINPDGIYIDVTFGGGGHSRAIF
                     EKLSPKGKLIVFDQDPDAKKNAWEAPNFFFVAANFSFLQNHLRLLGIKEVDGILGDLG
                     VSSHQFDEEQRGFSIRGNAPLDMRMNQNGELSAKNVINEYDEAELIRVFRNYGELKNA
                     RSVAQTIVSKRESKAIETTQDLMSAIEKHAPKFKDHKFYAQVFQAIRIEVNQEMEVLE
                     RFLKQTENVIKPGGRLVIMSYHSLEDRLVKNYIKTGNTAGKIEKDFFGNILKPFSEVV
                     RGIVTPTEEECERNSRARSAKLRIAEKVTTKK"
     gene            7887..8309
                     /locus_tag="PKN22_02245"
     CDS             7887..8309
                     /locus_tag="PKN22_02245"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNS41553.1"
                     /translation="MENEIIEKEVEQEEVKQPTQKAKKEKATSERKKPSLSVSQILNG
                     EFLTREFVLNNLNFIFFIILLLILIVAKGYYGKQLNDNVNKSQKELDEITAEYVEAKA
                     KLEEVTRRQILVEQLEARGLKETVNPTKVIRIREKKEK"
     gene            8309..>9487
                     /locus_tag="PKN22_02250"
     CDS             8309..>9487
                     /locus_tag="PKN22_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNS41554.1"
                     /translation="MDSKKGIYWRVYVVYLGFLLLMTLVLYKTAALQMEGRSEVFSAN
                     NGVQEKMPTRTVQRVPRRGEILDANYKQLVTSVSFYDIHMDPSVVDKELFDAEVNGLA
                     QGLFELFPDKTASEWEHYIRSAKASKKRYLLIKRKATIDERNQLLKLPIFNKGRLKGG
                     LIASEETIVRKRPHGELLKRTLGYFRPQEGSTGELKVGLEGAFNAYLAGENGEEVEQK
                     YGNGWKKTGQIIRDAVEGADVVSSIDLEIQEVAHTELENQLKHQNAKHGAVIVMEVKT
                     GFIKAIASLSRAEDGNYYETFNHALGTKEVPGSTFKLASLMALLEDKKVTINDEVNAT
                     GEYRFFNNVMKDHNGGFGKITIKQAFEHSSNVFTKIVYNAYKSNPMQYINRLRSFGLA
                     D"
BASE COUNT         3180 a         1719 c         1643 g         2945 t
ORIGIN      
        1 aattgcagca catggacctt ttgttatgaa cacatacgaa gaattagttg aagctttcaa
       61 tgattttaat caagggaagt ttggatcttt agaaaaataa ttacaatatt aaacctataa
      121 aaaaagggtg acttacacga gataagtcac ccttttgaat ataaattaga ataattattc
      181 tacaataatt ttttctactt ttttaccatt ttggttcact acttctacaa aatagattcc
      241 tgcatttgaa gcattcaaat caattgttgt agcagctgaa gatgattttg ttgcaatcac
      301 ttttccatta atatctgtta cagtaattgt attatccaag ttgttatcgt tagaaatatt
      361 taccaaacct tttgttgggt taggataaac tttaacacct tctaaattat caactgtttt
      421 aacagataac gaagggtcaa agttcatacg aattacagga actaaagaca aacctgtcca
      481 cgcaccagtt ccagagtttc caatgatcca tccccaagat tgagtagaac ctgaagtagc
      541 gccagaacct ccaaatacaa ctccttcatc taatacacgt acagaaatca tgtatgtacc
      601 aggagttaaa gattgtgggg tagagaacaa taaagatacg attgatgctt ttggtgtgct
      661 tgcagttaca gttaatggat ttgtatcatc gacatttagc cataaatcag aaccagatgt
      721 agcacttgta ttgtactgat acaaacttcc gtagaattgg ctaccgtttg ctgttgctgt
      781 atcaatatac acatcaatac cataaaggtc ttgtgctgta acaatgtcaa aagagttacc
      841 aatttctgtg taaggaattg tagcatctaa gaagatatct tctaaatcag ggtatgttga
      901 aaatggtgtt cccttatcaa ctgcataagt gtaattagtt acagaaaaag aacgatttgg
      961 taaaccagtg ttatttgctg ggttttcatc tgtttctgtt aaagataatg cttgagtaaa
     1021 attgtatgta cctacagctg caggtgtata agtagcaacc aacgtatcaa tttgaccaga
     1081 aacagaagta gcaactggtg ttgcagaaac agaagttgtt tcttgacctg taacatttac
     1141 tgttaaacca atgtttgtta agttattaat accattattt gttacaccaa caaaaaaatc
     1201 agttggttga acttgagtta aaggaatttg agaatattgg taaccctctg acccccagtt
     1261 ttggaaagtt acttttaagt cataatttgg tgtgcgtact attttcacat cgtcaaccat
     1321 ccatgcataa tcacatcctc cttgataacg gaaacaaatg tatgctgttg cagaaccacc
     1381 aataacaggt gctggaacag aaactgttgt agggttagca caagcatcat ttgctcctaa
     1441 gtctgtgtga actgctacgt atgtccaagt tgttccatca gttgaaaaac ctacataagc
     1501 gctcccttgg aattcacgat aataagattc aaattccaat gttacatttg gttcacctgt
     1561 taaatcaatt ggattaacat tttttaccaa tgcgttttga tctcctgaac acaataaatc
     1621 agagtcaaac tttgcaaaac catttgcagc tgatgttgat gcaattggag ctatcgcaaa
     1681 agttcccgca ggtccagttg ttccaattac ccagttatca ccacttcctg ctaaatcttg
     1741 gaaagcccaa tcagatgcag ttgagaaatc gtttgtccat aaatccactt tattttgatt
     1801 ctgaacaggc gtcttaactg ttttaactac agttatttct ttttgttttt gaatcgattg
     1861 cgccattgtt ccaaaagcga aacaaccgac aacaagcgtt aaataaattt gtttcataat
     1921 tgttaaatta aattgattga atgtaaaatt ataaaaaaat attaacgata caaaataatc
     1981 acaaactcaa aaagtaaaat aaactataaa ttaacaaaaa ttaaagacta taataggggt
     2041 gtgaaattca tacctacctt attcatgttt tcataacaga aagataccgc ttcatccaaa
     2101 gaatcaaaaa acacaaactc tgatgatgtt ttgtattttt tactgaaata tttgcctaca
     2161 attcgatcgg ccatgttttt agcaacaaca gcaacacaca acacatgctc tttaaatcca
     2221 atcttagtgt gttcaaccac attggaagag atagaaacaa aatcacctcc attcaaaatt
     2281 acagggtgaa gaactcctgg ttgctttaat tgaagtaaat acttaaaaat tgcatattgc
     2341 atttcttgat tcacttcttt tccctccttc acatagataa caataatgtt atccttcctc
     2401 cgcccaatgg tgaagtcttc caaatcataa aaatccatat acaaactgtt gctactcatt
     2461 tgttacaagt gcgaaataaa agtggctgaa attatataaa gtagtagtct tggtcggtac
     2521 aaatataatt aattttccga caaacaacca aataccatcg ataaataagt gttttttatc
     2581 gataaatacg attagtgagt gtttttaatg tttttaggtg agaaagggat tgcttaattg
     2641 tgattaagca atccccaatt ttattttttt atttcactaa actttttaca aagttaggtg
     2701 ttgcaaagct tcctttatga acgtcagcat tgtaataacg caatcctttt tgttcaacaa
     2761 aagcatcaat ttctgtttcg tttgaaatag attttggtgc tgcgcaacct tttaatccgt
     2821 attggaagct ccacattcct gttggatatg ttggaacaaa gaacaaggaa ataggtgcgt
     2881 tttctttacc aaaaattgat tgcaatgtgc ggttcaattc tgcaaatgct ttttcattga
     2941 attttggaga ctctccttgt gttaccaaaa tacctccatc tttcaaagca ttgtagcaat
     3001 tggtgtaaaa atcaacagag aataaacctt ctgccggacc aacagggtca gaaccatcaa
     3061 caataataat atcatacgat tttggtgcag cattttttac aaacgcaata ccatcgttta
     3121 caattaaatc caatttagga tgatcaaaag aagcggcaat ttgtggtaaa tgctctttac
     3181 atgctttaat tacttcacca tcaatttcaa ccatggttac tttttcaacg ccttcatgac
     3241 gaagaatttc acgcaccgtt ccaccgtcac caccaccaat tactaaaaca tttttagcat
     3301 ttccgtgggt aaacatcgct gggtgagaaa tcatctcgtg gtaatgaaac tcatcgttaa
     3361 tagttgtcat caccatatcg tctaatgtca acatttttcc gtatttagtt gattctaaaa
     3421 tacgcacacg ttgaaatggc gattgcacat cgtaaaacac atcacctgta aaacgtaacg
     3481 ataatgcttg gtcttcatct ttatctgtaa accaaacatt tcttgtataa aattcaggat
     3541 ttctccatcc cgaagcttgt tcacgcatac ttcttacatc aaaatcattg cgcgtcaata
     3601 aatttactga cccacgtttc atttcaatgg cagaatagga ttttgatttg aaacagtctt
     3661 tcaaatagtc aaacgcaatc caagcatcca tttctccaca agtaaataaa tctactgctg
     3721 cgtaaccgta ttcaggccac gtatgaatgg ccaggtgact ttcttggatg accactacac
     3781 cagatacacc ataaggcgaa aaatggtgaa atgttgagtt aataactgtt gcttcggctt
     3841 tttctgccgc ttcaaccatg ttacgctcaa ttgttgccac atcattcata atgtgtggat
     3901 cacattgcat aaactctacg agaatatgat ttccaagtgc tacgtttttc atttccttct
     3961 ttaaaaaatt taaaattagg ctacaaaagt agtgtatttt aacaacttat tacacattat
     4021 aaatggtaaa aagaaaattc ttttgtttct attctgtttt taaacaaatg gggatagtgt
     4081 ttttccattt cttgattcat taattcaatg ggcacatcat cgaattctcc ataaaaatga
     4141 atgtattcca taaattgatt ttgaccttca aattcttgaa acatagaatc ttgcactcca
     4201 tcccattcca acggggttac tttattaatt ctacacacat ttttatcaaa cgcaggagtg
     4261 catttcaccc atttctcgtc aacatacaac gatacataac catgaaaaac aatttctttt
     4321 ttaagtaaat atttctccaa tctatccgaa ccaatatggt taacaacaat ggcataacca
     4381 agtttagatg gaatccctaa tttgcgtgca gcagctgcca aaacaattcc tttttctaca
     4441 caccaagcgc gttttttagt taaaatagtg gaacctttca atgcatggtg gcgcaaatct
     4501 aaatgatgag gatcgtataa aaatccatct cttaccaaat aatacaactg aatggctttt
     4561 tccttatcac tcacggttgc atctattttt tctacaaaaa catcaaaaag tggatgcgaa
     4621 aaatctaaaa attgggtttc tttcaaaaaa ttgtgcataa tgcgtaactt tcaattattt
     4681 ttgggcaaac gtaaccatga cgcaagacga aacaaatata gaaaagaatg aattaaaact
     4741 gtttaattca aaaatgaaac aactcaaagt gggaattttt ggcagcgaaa aaccttccat
     4801 tttcactcaa atattgtttt attacggcac gttgatttca tttatttttg gtttgtggag
     4861 cgttatttct cttgcaatat taaaatttcc aaactatatc aaacaacaca aaaacgttga
     4921 tgttgaagcc atcattgaac tcagaggaag agaattaggt tttattaatg agagttttta
     4981 tgctaattta gaaaacttcc atttgtatag ctgtttcatt tggctcattt gttttacttg
     5041 ctttttcttg ctgtggcgta aaatttattg ggcaagctat gtgattgttg gcagcattct
     5101 cgtttactac acgttgatga tagtgatatt aggaagcact tattatgtgg aagacacaac
     5161 tatttttgac aaaactaccc ttctcctact gtttgtagca acaattgttc acgcactaat
     5221 ttatagaacc cctgaaaaga gtacgaatcc tgcaaaaact gaaaattaat tattttttta
     5281 ctgcggatag tgttatttca ctgcatttaa aatgaccttc tgggcaagct ttgaaaccat
     5341 gcaatccgca cggacggcaa tacaattctc ccacatccaa aatctgacta tcatcggaaa
     5401 gcggaccaaa accaaatgct ggaattgtag ataagaaaaa agctgtaacc ggtgcattca
     5461 tcgccgatgc aatgtgtaaa ggtcccgaat cgttcacata atttcgttgt gcatctttca
     5521 tcaatgctgc cgattctaat aaactcaatt tccctgataa attaaccacg tttgattgat
     5581 tacttgctgc aattattcgc tcacacaatt caccatccga tggcgctccc aataaataaa
     5641 tcgatgcctc ttgccctaat gtatgaatca actctaccca ttttttttcg ggtaattgct
     5701 tggtaaacca cactgatgca ggtgctaaac aataataggt tttttgctta tacattgcaa
     5761 ccttatcaaa ttgtttttca gaaggatata aaacaggacg cacttttttt actgcaccaa
     5821 actccaataa cagttgcata tttcgctcca cctcgtgcgt accatcgcct atggtgtgtt
     5881 caaacgattt tgaaaaccca aatgagaacg gatttttctt aaatccatac attttttttg
     5941 caccacttaa cagggttaaa attcccgagc taccaaaacg ttgaaaatta aaaacaatat
     6001 cgtattggcg ttttttaaaa acttttctca accgaaaaat ttccttgaat ttacctgctt
     6061 ttttatcaaa agtaaaaaca tgggtcagct gcggattgtt ttccaacaac gattcatttc
     6121 cttttttcac caccacatca atttcagctt ctggaaacaa gcgttttgct tccgaaaaag
     6181 cagctgtagc aagtattacg tcgcccaaaa atgcagtttg aataaataaa atacgcttca
     6241 cagtttttac ttagagataa caaagttaaa agaaaaaacg aacatcaaac cgataaaaat
     6301 agattgttaa ttacaaactt ttattcacaa ttaccacttt ttaccaccaa caatcaaaat
     6361 caataaaacg cttatttttc aatagttttt agctatttat tgaaactttt ttatctttgt
     6421 tacgttaata gttatcaaca atcactttaa aagtggtaaa aagtggtaaa atagttatta
     6481 tttttaccca ttatttgcat catggcagga ttagtaggag aatacgaagt aaaattggac
     6541 gctaaagggc gtttcttgtt tccggcaggt ttacggaagc aacttcctcc tgtatctcaa
     6601 aatgatttca tgttgaacaa aggatttgag gattgcttaa cactctatcc ccttgtagaa
     6661 tgggagaaaa tcagcgaaaa gttgtccaaa atgaatttgt ttaaaccaaa aaacagaatg
     6721 ttttaccgtt tgtttcatca aggagctaaa caagttgctt tggacaatgc gggacgaatt
     6781 ttaatacctt ctacgcatgt ggaacgtgtt ggaatcaaac aggatgtaat gctcattgct
     6841 tacaatgaca gaattgaaat ctgggataaa gctaaatact acgctcttat tgaagagaac
     6901 atggtggact tcgctgattt ggcggatgaa gtaatgggtg atttgaatga cgacacagaa
     6961 taacacatac cacgttcccg taatgttatc ggaatgcctt gaaggattga acatcaatcc
     7021 agatgggatt tatattgacg taacttttgg tggtggcgga cattcgcgtg ccatcttcga
     7081 aaaattaagt ccaaaaggaa aattaattgt ttttgaccaa gatccagatg caaagaaaaa
     7141 tgcatgggaa gctcccaatt ttttctttgt tgctgccaac ttttcgtttc tacaaaacca
     7201 tttgcgtctt ttaggaataa aggaagtgga tggtattttg ggtgatttgg gtgtttcatc
     7261 gcatcaattt gatgaagagc aacgcggttt ttcgattcgt ggcaatgctc cattggatat
     7321 gcgcatgaat caaaatggtg agctttctgc aaaaaatgta atcaacgagt acgatgaggc
     7381 ggagttaatt cgtgttttca gaaattatgg agaattgaaa aatgcccgtt ctgtggcaca
     7441 aaccattgtt tctaaacgtg aatcaaaagc cattgaaacc acacaagatt tgatgagtgc
     7501 tattgaaaaa cacgcaccaa aatttaaaga tcataagttt tatgcgcaag tttttcaagc
     7561 cattcgcatt gaggttaacc aagaaatgga ggtgttggag cgttttttaa aacaaacaga
     7621 aaatgttatc aaacctggag gtagattggt aatcatgtct taccattcat tggaagatag
     7681 attggtgaaa aactacatta aaacaggaaa tacagctgga aaaatagaga aagatttttt
     7741 tggcaacatt ctaaaacctt tttcagaggt ggtgcgagga attgtaacgc caactgaaga
     7801 agaatgtgaa cgtaactcgc gtgcacgaag cgctaaatta cgcatagcag aaaaagtaac
     7861 tactaaaaaa tagccgtttc tacgcaatgg aaaacgaaat tatagaaaaa gaagttgaac
     7921 aagaagaggt gaagcaaccc actcagaagg ctaaaaaaga aaaagccact tctgagcgca
     7981 aaaaaccttc actttcggtg tcgcagattc tcaatggtga gtttctgaca cgtgagtttg
     8041 tgctcaacaa cctcaacttt atctttttta taattctctt attgattttg attgtggcaa
     8101 aaggatatta cggtaaacaa ttgaacgata atgtcaacaa gtcgcaaaaa gaattggatg
     8161 agattaccgc agaatatgta gaagcaaagg caaaattgga agaagttact agaagacaaa
     8221 ttttggttga acaattagaa gcacgtggat taaaagaaac ggttaatcca acaaaagtga
     8281 tacgcatacg agagaaaaaa gaaaaataat ggactctaaa aaaggcatat attggcgtgt
     8341 ttacgtggtg tacctcggtt ttctattact gatgactttg gtgctctaca aaacggctgc
     8401 gcttcaaatg gagggtcgct ctgaagtgtt ttctgccaac aatggtgtgc aagaaaaaat
     8461 gcctacacgc actgttcaac gtgtaccacg tagaggagaa attttggacg ctaattataa
     8521 acaacttgta acctctgttt ctttttacga tattcacatg gatccatcgg tagtagataa
     8581 agaattgttt gatgctgaag ttaatggttt ggcgcaaggt ttatttgaat tgtttcctga
     8641 taaaactgct agtgagtggg aacattacat tcgttctgct aaagcttcaa aaaaacgtta
     8701 tttactcatc aaacgcaaag ccaccattga cgagcgcaat caattgttaa aacttccaat
     8761 tttcaataaa ggccgattaa aaggtggtct catagcttct gaagaaacaa ttgtaagaaa
     8821 acgcccgcac ggtgaattat taaaaagaac attgggttat tttagaccgc aagaaggtag
     8881 cactggcgaa ttaaaagttg gattagaagg tgcattcaac gcatacttag ctggtgaaaa
     8941 cggtgaagag gtagaacaaa aatatggcaa cggttggaaa aaaacgggac aaataatccg
     9001 cgatgccgtt gaaggtgctg atgtagtttc ctccatcgat ttggaaattc aagaggtggc
     9061 tcataccgaa ttggaaaacc aattgaaaca tcaaaacgca aaacacggag cggtgattgt
     9121 gatggaagtt aaaacaggtt ttatcaaagc aattgcttct ctttcaagag ctgaagatgg
     9181 aaactattac gaaacattta accatgcatt gggaactaaa gaagtgcctg gttctacctt
     9241 caaattggct tcattgatgg ctttgttgga agataaaaaa gtgacaataa acgatgaagt
     9301 aaatgccact ggagaatacc gcttttttaa caatgtgatg aaagaccaca acggtgggtt
     9361 tggcaaaata accatcaaac aagcatttga acactcttcc aacgtgttta ccaaaattgt
     9421 ttacaatgca tacaaaagca atcctatgca atacatcaac cgtttgcgca gttttggatt
     9481 ggcggat
//
LOCUS       DAOQVQ010000039         9348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8219_length_9348_cov_4.274400, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000039 DAOQVQ010000000
VERSION     DAOQVQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8219_length_9348_cov_4.274400"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKN22_02255"
     CDS             <1..288
                     /locus_tag="PKN22_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HNS41555.1"
                     /translation="RVQDALDQLWRYTDELFYEDNVDAELQAAGIIPKMADIKAEWSK
                     TVTAVLEEATLTIPDNGWSYGKGRTGLHSENLGYILADMQYLQRAYPGATW"
     gene            310..795
                     /gene="paaJ"
                     /locus_tag="PKN22_02260"
     CDS             310..795
                     /gene="paaJ"
                     /locus_tag="PKN22_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNS41556.1"
                     /translation="MYSEEEIWDFMEEVFDPEVPVLTLVDLGVVRKVNVDGEKVKITI
                     TPTYSGCPAMRVMEQDIIALLHSKGIENCEVETVLAPAWTTDWISENGRIKLKEYGIA
                     PPADELDKSVLFGKQPTVECPRCNSTNTHMISQFGSTSCKAQFRCKDCLEPFEYFKCL
                     K"
     gene            941..1147
                     /locus_tag="PKN22_02265"
     CDS             941..1147
                     /locus_tag="PKN22_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41557.1"
                     /translation="MLELTKKVLKKVSFDAKLFQKELYKALKWMKDTEEIKSFQKWCI
                     VEFGTIYPSILKEAFGKNALVKIK"
     gene            1252..2523
                     /gene="serS"
                     /locus_tag="PKN22_02270"
     CDS             1252..2523
                     /gene="serS"
                     /locus_tag="PKN22_02270"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNS41558.1"
                     /translation="MLEITRIRTEKDAIIEGLKKRHFDAKPIVDEILAKDEQWRSLKA
                     SMDQILSEMNAYAKNIGLLMKEGKKEEAENAKAEVSKLKEHEAELKVKVDALDAEIQQ
                     LMYLLPNVPNEQVKAGKNELDNETVYEWGQAPTYNFEAQAHWDLAKKYDLIDFELGVK
                     VTGAGFPFYKGKGAKLQRALITFFLTEAEKAGYTEYISPHVVNEASGIGTGQLPDKEG
                     QMYQIPIDGLYMIPTAEVPLTNIYRDVILPSEDFSIKMTGYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVRIEHPSNTAKALEEMLAHVKNLLEKLGLQYRILRLCGGDTGFASA
                     MTYDFEVYSAAQQKWLEVSSVSRFDTFQANRLKLRFKTADKKTQLAHTLNGSALALPR
                     ILASILENHQTADGIVIPEVLRPFTGFDKID"
     gene            complement(2579..3511)
                     /locus_tag="PKN22_02275"
     CDS             complement(2579..3511)
                     /locus_tag="PKN22_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41559.1"
                     /translation="MKSKGWIFILLIVAFSCSSENDTIKLFKKDKVNNNLLNLAIFQS
                     ELESMLAFPVLFNDSIVKKNKIATIKRKQLYKHNENDGEVSEVETDKIITYFFDENGH
                     NSKIKVERFYDIKSIEKNIIHFSEYDPNKGYAKYQINNSTPMDESTYANYQLANKQQN
                     YIKFHNDLKGNNLYLIPASKFWKPLVIDTMCNPQKQDVIVLGTLRKPHKIYSVKDLVN
                     ETNVRKFTYKHNLLKKIVWDDQPFTVNREFEYNDKGICIGFTDKVYSIKALVNKTHYS
                     ITLKGSLPIKVTKEIRVGNKLLTIYEEMFTYTYN"
     gene            complement(3501..4361)
                     /locus_tag="PKN22_02280"
     CDS             complement(3501..4361)
                     /locus_tag="PKN22_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNS41560.1"
                     /translation="MAFSKKYTSKDKSLFKQRLYIIMFEAKTTAGKKFDLYLMGLIVF
                     SLFILMIESLPSLSPTTRLIFYVAEWVLSSIFFIEYLLRVYVSPKPLKYILSAWGIID
                     LLSVLPIFFLAFNNTAHYFRIIRILRLIRVFKIFRLSQFTKEAYSLYHSLVASLYKIA
                     VFMFFVILIAIIMGGLMFIVEGGGENGFNSVPESIYWAIVTITTVGFGDIAPVTDFGK
                     FLASIMMLLGYAIIAVPTGIVSMEMYKNQEKANNEIVSCPNCSNVNPKQANYCNHCGA
                     SIDKKQEDEK"
     gene            complement(4530..4603)
                     /locus_tag="PKN22_02285"
     tRNA            complement(4530..4603)
                     /locus_tag="PKN22_02285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4567..4569),aa:Arg,seq:tcg)
     gene            complement(4618..5289)
                     /locus_tag="PKN22_02290"
     CDS             complement(4618..5289)
                     /locus_tag="PKN22_02290"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNS41561.1"
                     /translation="MTIHREGYTIILSGLVITALLQFGNYKLYQFLGWTWLYILLTLG
                     ILTLLYLIIQFFRVPNRKAIYYSNDILCPADGKVVVIEEVEETEYFNDKRIQISIFMS
                     PLNVHANYNPISGIVKYVKYHKGLFLVAWHPKSSTDNERSTFVVQHESGQEVLFRQIA
                     GAVARRICYYAKENQQVKTGEEFGFIKFGSRIDLFLPLGTKIDVNIGDVVKAKLTKIG
                     ELKVD"
     gene            complement(5296..6117)
                     /locus_tag="PKN22_02295"
     CDS             complement(5296..6117)
                     /locus_tag="PKN22_02295"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNS41562.1"
                     /translation="MNNILVRTISGSIFISLILVPLFLANYNYAILTLCAFLFLGLDE
                     YANLFKNSAYISINKTLNTFFTAIIVSLLLLVIVPNQLFEFNALIALFPLFFLWFLTE
                     LWRKKEHPLLNIGVSFFGFIYLCIPFLLAIDIHIQDTHAFPKLVGMFVLIWTNDTFAF
                     LSGKFFGKHKLFERISPKKTWEGTIGGVLLTLLMAFTLTELFDKENQLFWMVSAVLIS
                     TTAIFGDLFESLFKRSLNIKDTGNIIPGHGGILDRFDAFLFTVPFFYFWCQIYFN"
     gene            complement(6218..7090)
                     /locus_tag="PKN22_02300"
     CDS             complement(6218..7090)
                     /locus_tag="PKN22_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS41563.1"
                     /translation="MNNSEILHCIVCGHNELIPLDRYKDAHLCQCLNCSLVFSTQKPS
                     HEELENEQKKLKRKDRITKDAIKRYHHILDRFEGYRKNNRLLDLDCNHGEFLQMAKER
                     GWYVYGTSDTEESYNECVAKGLNMFKGSLKLDNFEDNYFDVVCARNILEHLTEPNEEI
                     KKIKRIIRRKGLLYATTPNFNSYLRYRLKEKYSLISYPLRLVYYSRKPFKRLFRQNGF
                     KVLETEAPWVSISNIFVSRAKTQVPLTEKSEFNEEDKDLIGKRYRRFIKRTLNPVLGF
                     LGFGDYLKGWFVKL"
     gene            7240..8817
                     /locus_tag="PKN22_02305"
     CDS             7240..8817
                     /locus_tag="PKN22_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNS41564.1"
                     /translation="MRTLIIFTLISSIGFSQTEKHYPPIPFDSTLYQNKQQLLQQSTQ
                     PNANAKNIILIVADDLGKYDLSCYGNPFIKTPHIDQLAKDGVLYDQSYATAAVCSPSR
                     AGFMTGRYQQRFGYHVQPHQRYPKNKFELWAFQHLINTNDLHPEDFAKYPSKENIAKA
                     GLPQSELTISELLKANGYKTAWIGKWHLGYTEPLLPKNFGFDYRYGCLEAYTLFANPK
                     DKTIVNAKIKEFTDKVIWKGGRKDACAIRENEVIIDEKEYITYAFFNRAKQFIGKNIS
                     NPFFVYLPITAPHTPYQAPKEIYDQLTHISDHNKRVYYSMIIALDNAIGELITYLKSN
                     NLYDNTLIIFTSDNGAALYTNTIDNKPLNGGKFTFFEGGINVPLIIKNDISTYFDITK
                     INAPVSLLDLFPTIADAANIPLPSDRTYDGISLLQYNTNEKILEKRNTLYWYSDYNAA
                     IRQGNEKLILNSLDNTVDFYNLATDPYELNNISTPNATSLKLNLLQWMKKMPPMYWPR
                     IMNYELIINGKKYRWAV"
     gene            8805..>9348
                     /locus_tag="PKN22_02310"
     CDS             8805..>9348
                     /locus_tag="PKN22_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNS41565.1"
                     /translation="MGCLIFLLLFTCSLSSAQNSNPLKFVKGGTIQIGSNEYADIQPE
                     FKARIQSFWMQEHEVTNQEFANFVEQTNYVTLAEKNGGSYVFDSKRKTDSLTLENAPW
                     WYFSKTANWKNPYGKDTVIGNFMELPVVHISHQDASCYCNWLGMRLPTEIEREYVGRK
                     NGTSQNRNTWQGDFPSSNLLE"
BASE COUNT         3266 a         1622 c         1493 g         2967 t
ORIGIN      
        1 cgtgtgcaag atgcgttgga tcaattatgg cgctataccg atgaattgtt ctacgaagac
       61 aatgttgatg cagaattaca agcagctggt attataccta aaatggctga cattaaagct
      121 gaatggtcaa aaactgtaac tgctgtattg gaagaagcaa ctttaacaat tccagataat
      181 ggctggtcgt atggtaaagg tagaactgga ttacattcag aaaacttagg atacatcttg
      241 gctgatatgc aatatttaca acgcgcatat cctggagcaa cttggtaata aaaaacaaaa
      301 ggttttaaca tgtactcaga agaggagatt tgggatttta tggaggaggt ttttgacccc
      361 gaagtacctg ttttaacatt ggttgacctt ggtgttgtaa gaaaagttaa tgttgatggt
      421 gagaaagtaa aaatcaccat cactccaact tattctggct gccctgctat gagagtaatg
      481 gagcaagaca tcattgcttt gttgcattca aaaggaattg aaaattgtga ggttgaaacg
      541 gtgctagcgc ctgcttggac aaccgattgg atatccgaaa atggtcgaat taaacttaaa
      601 gaatacggca ttgcaccccc agctgatgag ttagacaaga gtgtattatt tggaaaacaa
      661 ccaacagtag aatgtccgcg ttgcaattca acaaacaccc acatgatttc tcaattcgga
      721 agcacctctt gcaaagcaca atttcgatgc aaggattgtt tagagccttt tgaatacttc
      781 aagtgtttga aataaaattc attatccaat attttattaa aagagtggtt ggcttccttc
      841 cactcttttg tgtttttttg tgaattaatt aacgaaataa attagcaaaa atccatcttt
      901 atttctaatt ttaaaatgta ataattttaa tacagaaatc atgttagaac tcactaaaaa
      961 agttttgaaa aaagtgagct ttgatgcaaa actctttcaa aaggaactat acaaagcctt
     1021 aaagtggatg aaagatactg aagaaattaa gtcttttcag aaatggtgca tcgttgaatt
     1081 tggcaccatc tacccttcca tccttaaaga agcatttgga aaaaatgcat tagtaaaaat
     1141 aaagtagtat taatttcata aaaattaaga gcattttaat cagaaatctt tctcgattat
     1201 tatgctcttt ttctttttat ccttactttc gcagtagatt aaagaataga tatgttagaa
     1261 attacacgta ttcgcaccga gaaagatgca attattgaag gtctgaagaa aagacatttt
     1321 gatgccaaac caattgttga tgaaatttta gctaaagatg aacaatggcg ttcgttaaaa
     1381 gcttcgatgg atcaaattct ttccgaaatg aatgcttatg caaaaaacat aggtctatta
     1441 atgaaggaag gaaaaaaaga ggaagcagaa aatgcaaaag ctgaagttag caaactcaaa
     1501 gaacatgaag ctgaactaaa agtaaaagtt gatgctttgg atgctgaaat tcaacaattg
     1561 atgtacttac ttccaaatgt tcccaacgaa caagttaagg caggtaagaa cgaattggat
     1621 aatgaaaccg tttacgagtg gggacaggca ccaacttata attttgaggc acaagcacac
     1681 tgggatttag ctaaaaaata cgacctcatc gattttgaat tgggagtaaa agttacaggc
     1741 gctggttttc cattctacaa aggtaaaggt gcaaaattac aacgcgcatt gattactttt
     1801 ttcttaactg aagccgaaaa agcgggttat acagaatata tttctccaca cgttgtaaat
     1861 gaagcttctg gtattggaac tggtcagctt cctgacaaag aaggtcaaat gtatcaaatt
     1921 ccaattgatg gtctatacat gattccaaca gccgaagtac cattaaccaa tatttatcgc
     1981 gatgtgatcc ttccgtcaga agatttttct ataaaaatga ctggatatac accttgtttt
     2041 agacgtgaag ctggttctta cggtaaagat gtacgtggac ttaatcgttt gcatcaattt
     2101 gataaagtgg aaattgtgcg cattgaacac ccatccaaca cagcaaaagc attggaagaa
     2161 atgttggcgc atgtgaaaaa tttattagaa aaattagggt tacaataccg tattttaaga
     2221 ttgtgtggtg gtgacactgg ttttgcttcg gcaatgacgt atgattttga agtgtactca
     2281 gctgctcaac aaaaatggtt agaagtctcg tctgtttcca gatttgatac attccaagca
     2341 aaccgtttga aattacgttt taaaacggct gataagaaaa cacaattagc acatacactc
     2401 aatggatctg cattggcttt acctcgaatt ttagcatcta ttttggaaaa tcaccaaacg
     2461 gcagatggaa ttgtaatacc agaggtttta cgtccattta cagggttcga taaaatagat
     2521 taaaaaaaca taaaattgaa ataaattaag actgattggc aatgctggtc agtctttttt
     2581 aattatacgt ataggtaaac atttcttcgt atatcgttaa caacttattt cctacacgaa
     2641 tttcttttgt aacttttatt gggagagaac ctttcaaagt tatgctgtaa tgcgttttat
     2701 tcaccaatgc tttaatgcta taaactttat ccgtaaaacc tatacaaatt cctttatcgt
     2761 tgtattcaaa ctctctattt acagtaaagg gttgatcgtc ccaaacaatt tttttcaata
     2821 gattgtgctt ataagtgaat ttgcgaacat tggtttcgtt cactaaatct tttacactgt
     2881 atattttatg aggtttgcgc aaggttccca aaacaataac gtcttgtttt tgaggattac
     2941 acatggtatc aattaccaat ggtttccaga attttgaagc aggaataaga tataaattat
     3001 tgcctttcaa atcattgtgg aatttaatat aattctgttg cttattcgcc agctgataat
     3061 tggcataagt tgattcatcc attggtgtgg aattgttgat ttgatatttt gcataccctt
     3121 tattgggatc atattccgaa aaatggataa tatttttttc aatggattta atatcgtaaa
     3181 acctttcaac tttaattttt gagttgtgtc cgttttcatc aaaaaagtag gtaattattt
     3241 tatctgtttc aacttcagat acttcaccat cattttcatt gtgcttatac aattgttttc
     3301 tttttatggt ggctatttta ttctttttta caattgaatc attgaacaga acaggaaaag
     3361 ccagcatact ttctaattct gattgaaaaa tagccagatt taacaaattg ttatttacct
     3421 tatccttttt gaataattta attgtgtcat tttcagaaga acatgaaaaa gcaacaataa
     3481 gcaaaataaa tatccatcct ttacttttca tcttcttgtt ttttatctat gctagcacca
     3541 caatggttgc aatagttggc ttgttttgga ttaacattag aacaattggg gcaacttaca
     3601 atttcattat tagccttttc ttgattttta tacatttcca tcgatacaat acctgttgga
     3661 actgcaataa tagcataacc taataacatc attatagatg ctagaaattt accaaaatct
     3721 gttactggtg caatatcacc aaaaccaact gttgttattg ttacaatggc ccaataaata
     3781 ctttctggaa cagagttaaa tccgttttct ccaccacctt caacaataaa cattaatcca
     3841 cccataataa ttgcgatcaa aatcacaaaa aacataaaca cagcaatttt atacaagctc
     3901 gccaccaatg aatggtataa tgaatatgct tctttagtaa actggcttaa tctaaagatt
     3961 ttaaaaacac gaatcaatcg caatattcga ataatacgaa aatagtgagc cgtattatta
     4021 aacgccaaaa agaagattgg taaaaccgat agtaaatcta ttattcccca agcacttagt
     4081 atgtatttta aaggtttggg agaaacataa acgcgcaata aatattcaat aaaaaagatg
     4141 ctagacaaca cccattctgc aacgtagaaa atcaaacgag ttgttggtga caaggagggt
     4201 aagctttcaa tcattaaaat gaataatgaa aaaacaatta accccatgag gtataagtcg
     4261 aactttttac ctgcagttgt ttttgcttca aacatgatga tgtataaccg ttgtttgaaa
     4321 aggcttttat cttttgatgt gtattttttt gaaaatgcca tggtttgcta aaaacttact
     4381 aaaagtaata aataaatttt aaacgttgaa tttatttatg ttatcgagat atcgaactat
     4441 ttcgatatcg caatattgcg atataacgat attgtgtcaa aaatcataca aaaaacaaaa
     4501 gccaaccact ttaatgattg acttttatgt gaccccggag ggattctaac ccccaaccgc
     4561 tggagccgaa atccagtgct ctatacagtt gagctacgga gcctaattaa attgtaatta
     4621 atcgactttt aattcaccaa tttttgtcag ttttgctttt acaacgtcgc caatatttac
     4681 atctattttg gttcccaaag gcaagaataa atcaatgcgc gaaccaaatt ttataaatcc
     4741 aaactcttct cctgttttta cttgctgatt ttcttttgcg tagtaacaaa ttcttcgggc
     4801 aactgctcca gcaatttgtc taaacaatac ttcttgtcca ctttcgtgtt gaacaacaaa
     4861 tgtactgcgt tcattgtcgg tgctactttt tggatgccat gctactaaaa acaaaccttt
     4921 gtggtatttt acatatttaa caattccaga aattggattg taattggcat gtacatttaa
     4981 aggcgacatg aaaattgaaa tttgaatacg tttatcgtta aaatattctg tttcttcaac
     5041 ttcttcaata actacaactt tcccatcggc aggacataat atatcgttgc tataataaat
     5101 tgctttacgg tttggaactc taaaaaattg aattattaaa tacaacagtg ttaaaattcc
     5161 aagagttaat agtatgtata accacgtcca ccctaaaaat tgatacaatt tataatttcc
     5221 aaattgaagc aatgccgtga ttactaatcc tgacaaaatg attgtgtagc cttctctatg
     5281 tatggtcata atttattagt taaagtatat ttggcaccaa aaataaaaaa atggaacggt
     5341 aaataaaaat gcatcaaatc gatccaaaat accaccgtgt ccaggtatga tgttgcctgt
     5401 atcttttatg tttaaacttc ttttgaaaag cgattcaaat aaatcaccaa atattgcagt
     5461 tgttgaaatt aaaaccgcac ttaccatcca aaacaactga ttttctttat caaacaactc
     5521 tgtaagagta aacgccatta aaagggttag taaaactcct cctatagttc cttcccaagt
     5581 ttttttaggt gaaatgcgct caaataattt atgtttacca aaaaatttac ctgataaaaa
     5641 tgcaaaggta tcgttggtcc aaatcaatac aaacatccca accaattttg gaaatgcatg
     5701 tgtatcttga atatggatat caattgctaa taaaaatgga atgcacaaat aaataaatcc
     5761 aaaaaatgat acaccaatgt ttaacaatgg atgttctttt ttgcgccaca attctgttaa
     5821 aaaccaaaga aaaaataaag gaaacagagc tattaaagca ttaaattcaa ataactgatt
     5881 cggaacaatt actaacaata gtagtgaaac aataatggca gtaaaaaaag tgttcaacgt
     5941 tttgtttatt gagatataag cagaattttt aaacaaattt gcatattcat ccaatcccaa
     6001 aaataaaaat gcacacaatg taagaatagc atagttgtaa tttgctaaaa acaaaggcac
     6061 aagtattaag ctaatgaaaa tagatccaga aattgttctg actagaatat tattcataga
     6121 ttaaaatttg gggtaaagat agtgggtaaa tatgaatttg agatgattta tctaaatatt
     6181 tgcatatcgc aatattacga tatggtaaaa taaagaacta aagtttcaca aaccaaccct
     6241 ttaaataatc accaaatcct aaaaacccta aaacaggatt taacgttctc ttaataaatc
     6301 ggcgatatct ttttccaatt aaatctttat cttcttcgtt gaactccgat ttttcggtca
     6361 aaggcacttg agttttagct cgtgaaacaa atatattcga aatactcacc catggagctt
     6421 ccgtttctaa tactttaaat ccattttgtc taaaaagtcg cttaaaaggt tttctggaat
     6481 aataaaccaa acgaagtgga tacgatatta aactgtattt ttcttttaac cgataacgca
     6541 aatatgaatt gaaattggga gttgttgcat ataataaacc ttttcttcga ataatacgct
     6601 ttattttttt aatctcttca tttggttcgg ttagatgttc caaaatattt cttgcacaaa
     6661 caacatcaaa ataattgtct tcaaaattat ctaatttcaa agagccttta aacatattta
     6721 acccctttgc tacacattca ttgtaagact cttcagtgtc tgatgtacca taaacatacc
     6781 acccacgctc ttttgccatt tgcaaaaatt caccatggtt acaatctaaa tccaacaagc
     6841 gattgttttt tctataacct tcaaatcgat ctaaaatgtg atggtagcgt ttaatagcat
     6901 ccttggtaat tctatccttg cgtttcaact ttttttgttc gttttccaac tcttcgtgac
     6961 taggcttttg tgttgaaaac accaaactgc aatttaaaca ttgacataaa tgagcatcct
     7021 tatacctgtc caaaggtatg agctcattgt gtccacacac aatgcaatgt agaatctcac
     7081 tattattcat cgaatacaat tacgttaatc tacctgattt gtttcaaatc taatttgttt
     7141 ttttctgtaa aataatacca tatcgcaata tcgaactatt tcgatatcac gattcaattc
     7201 gatttatttt atctttgctt tcaacttaaa acaaacaaat tgagaacgtt aatcattttt
     7261 acacttattt catccattgg gttttctcaa acagaaaagc attaccctcc cattccattt
     7321 gattcaactt tgtatcaaaa taaacaacaa ttattgcagc aatcaacaca gccaaacgcg
     7381 aatgcaaaaa atataatttt aattgtagct gatgatttag ggaaatacga cttatcatgt
     7441 tacggcaatc cttttataaa aactccacat atcgaccaac tagcaaaaga tggagtttta
     7501 tacgatcaaa gttatgccac tgctgctgtt tgtagcccgt caagagctgg atttatgaca
     7561 ggacgctatc agcaacgttt tggttatcat gtgcaacctc atcaacgtta tcctaaaaat
     7621 aaatttgaac tatgggcatt tcaacaccta attaacacca acgatttaca ccctgaagat
     7681 tttgctaaat atccttcaaa agaaaacatt gcaaaggcag gtttaccaca atctgaacta
     7741 acaatttctg aattattaaa agcaaatggc tacaaaactg cttggatagg aaaatggcat
     7801 cttggttata cagaaccatt gttacccaaa aattttggtt tcgactaccg ctatggttgc
     7861 ttagaagctt acacactttt tgcaaatccg aaagataaaa ctattgtcaa tgcaaaaatc
     7921 aaagaattta cagataaagt aatttggaaa ggtgggagaa aagatgcgtg cgccattcgc
     7981 gaaaatgagg tgatcattga tgaaaaagaa tacatcacct atgctttttt taatcgagca
     8041 aagcaattta ttggcaaaaa catttcaaat ccgttttttg tttacttgcc tattactgcg
     8101 ccccacacac cttatcaagc gcctaaagaa atttatgacc agttaacaca tattagcgac
     8161 cacaacaaac gagtttatta ctcaatgatt atagcactcg acaacgccat tggagagtta
     8221 attacttact taaaatcaaa caatctgtat gacaatacac ttattatttt caccagtgat
     8281 aacggagctg ctttatacac aaatacaatt gataacaaac cacttaacgg agggaaattt
     8341 acgttttttg agggtggaat aaatgtgcct ttaatcatca aaaacgacat atcgacatat
     8401 ttcgatatca cgaaaataaa cgctcccgtc agcctcctgg atttattccc aaccattgcc
     8461 gacgctgcca acattcctct tccctccgac cgaacatacg acggaatttc attactccaa
     8521 tacaacacca acgagaaaat tcttgaaaaa agaaatacac tttattggta ttcggattac
     8581 aatgctgcta tccgtcaggg aaatgagaaa ctaatactca attctttaga caataccgtc
     8641 gatttttaca atttagcaac cgatccttat gaattaaaca acatcagcac tccaaatgca
     8701 acctcgctaa aactcaacct attgcaatgg atgaaaaaaa tgccccctat gtattggcca
     8761 cgcattatga attacgaatt aataatcaat gggaaaaaat acagatgggc tgtttaatct
     8821 ttttgcttct gttcacgtgc tcgctaagtt ctgcacaaaa cagcaatcca ttaaagtttg
     8881 taaaaggcgg aactatacaa attgggtcga atgaatatgc ggatattcaa ccagaattta
     8941 aagcacgtat tcaatcgttt tggatgcaag aacacgaagt caccaatcaa gagtttgcaa
     9001 actttgtgga gcaaaccaat tatgtaacat tagctgaaaa aaatggaggt tcttatgtat
     9061 ttgattcaaa aagaaaaaca gactcgctta cacttgaaaa tgctccgtgg tggtattttt
     9121 ccaaaactgc aaattggaaa aatccgtatg gaaaagatac tgttattgga aattttatgg
     9181 agcttcccgt ggtgcacatt agtcatcagg acgcaagttg ttactgcaat tggttaggaa
     9241 tgcgcctacc taccgaaata gaacgtgaat atgttggaag aaaaaatgga actagccaaa
     9301 atagaaatac atggcaaggc gattttccaa gtagcaattt gttagagg
//
LOCUS       DAOQVQ010000040         9289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8286_length_9289_cov_3.791964, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000040 DAOQVQ010000000
VERSION     DAOQVQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8286_length_9289_cov_3.791964"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PKN22_02315"
     CDS             complement(<1..1121)
                     /locus_tag="PKN22_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HNS41566.1"
                     /translation="MKRIIKSALVLGTFTLFSNMSLAYLYTGSSNVQPKGNDKVKPSN
                     SEKGAACAPAVKKYILDFNDVSALLQMGGRLFEDEQKSVAGYEVPKGGGAKAIFAAAL
                     WMGGRDQNGQLKLAAVRFRQVGNDFWGGPLTVTIPYAPNVNYNPLNPVGDNTIRPYGD
                     GNISGDQCAKYDKFFPINKASVIRFSIWWESCIDPNKAPGACDLIGEESLPTNAELAA
                     ITEWPAHGDPSLFQDYYLAPFYDRNADQIYNPMDDGDYPWYDDILGRDDVQCGVDRRV
                     TLFGDQTVWFVFNDKGNVHTESQGEPIGMEIRAQAFAFATSDEINRMTFYNYELINRS
                     TQTLTNTFFSQYVDPDLGYSGDDYVGCDVSRGLGYCYNGT"
     gene            complement(1142..4906)
                     /locus_tag="PKN22_02320"
     CDS             complement(1142..4906)
                     /locus_tag="PKN22_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNS41567.1"
                     /translation="MLKNLHLVILSVLLSVAYSFSQSGLGTIRGSVIDGETRGPIGFA
                     KVLLLQDGTVKGGANTDDDGKFQINSIAPGSYDVEVRNEGDGYQPTAIRGVIVNADKI
                     TFLDNTEISKPKDVQNIEELVVVAYKVPLINKDGGASGSTITAEDISRLPVRSAAGVA
                     QTVGGVNTDEGSGAISVRGSRSDATYYYIDGIKVRGSSNLPKSAIEEVSVITGGLPAN
                     YGDVTGGVISVTTRGPSSKFFGSIEAVSSGFYIKGKDKDGYDGKVFGLDKYAYNLVEG
                     MISGPLFMKKDSTGAKTKPIMGFLLSANYLNELDNRPLNGGSYRVKKEIRDSLLQYPL
                     VMNANGDGTFYRATLLKENDFEKTKWRMNARSQNVSAQAKIDVNTGPSVNLSFGGSMN
                     YYDRSLYDRTSSLLNFQNFGRGKNLDWRVFGRLSQRFSNKREGSASKVKSAFYSLMFD
                     YSQSRSKSYDPKHGFNMFNYGHVGTFTSRFDTSFVFVDSLNAYVNQEVPFQNGVTFTG
                     SETNPGLTALTSQYYDLFGSAANFNQLRQGNALLNGDAPISVYQIWNNIGTPYNGFGI
                     SENNQLRVTGSGSINVGDHSISLGFEYEQRTDRGWSSGDNGPIGLWSLARLLANSHIS
                     EIDKNDPNPTISQEAGFQKIYYNRLNTGYAHNHNGQFGGAQSGASGGQVDYQSFFDYN
                     VRKELGLDPGGNDYIQIDAMDPSRLNFGMFAPDELLNNGKSYVSYYGYDHAGNKVKGR
                     TDIDKYFNEFDENGNYKRFVGAFSPTYISGYIMDKFAFRDLVFNVGVRVDVFDANQPV
                     LKDKYLFHSALTAGEARAKMNSTDYSWVNVPTSIGDDYTVYVDDVAAPTQINGFRNGD
                     VWYNANGDQVEDPAVLRGANGIAPWLADAGKTTISSSAFKDYKAQVSVMPRIAFSFPV
                     SEESSFFAHYDILTKRPTTGVRFDPYEYQFIQTRSEIINNSNLKPEKTIDYELGFQQV
                     LTRTSSLKISAFYREQRDNVQLINVVEAYPATYKTFGNIDFGTVKGLTVAYDLRRTGN
                     IRMTAAYTLQFADGTGSDATSMAGLINAGLPNLRAIFPYSFDQRHAFAISFDYRYGTG
                     KDYNGPRIKNYEVFGNMGLNIMANIYSGNPYSKKLDIVNEGMFSPQASGLDGTTNGSR
                     LPWSYRLDIQLDRTFEFEVGKKEDKKRTTYLNVYLRVTNLLNQKNILSVYRATGNWND
                     DGYLEAASSQTSIQNQTDEASFRNYYLMKIQNPYNISAPRTIRLGIKFDF"
     gene            5215..5847
                     /locus_tag="PKN22_02325"
     CDS             5215..5847
                     /locus_tag="PKN22_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNS41568.1"
                     /translation="MFYLKNIKIGDSTLYFIRFDEYNINDYTYLLTNDEQFKLSTFKS
                     EKRKKEFVATRILKTTLFKNTPIYYLSTGAPYFKNSTNHISISHCDNWVVFAVCNCYK
                     IGVDIEPISSKAQRLHQKFLNESEIALLDTSNIELMSKAWSCKEALYKLSTKEGIIFK
                     KDLLITDFDKKNTFNCKVLEENKSFSVHLSASIIDGYIVTINTSPLYENH"
     gene            5834..6559
                     /locus_tag="PKN22_02330"
     CDS             5834..6559
                     /locus_tag="PKN22_02330"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNS41569.1"
                     /translation="MKIIDSIIDQQKIAVLIDPEKCQSIAYIEQLIEKIHFSGVDFIF
                     IGGSTVSEKEFNQTIAALKKLARIPIIIFPGSSSQVSPLADAILLLNLISGRNADFLI
                     THHVQAAQKIVDAGLEVISTSYLLIDGGSHSSVAYVSQTTPIPQHQLSIATQTALAGK
                     LIGHQLVFMDAGSGAKNCVSTEMVKAVKQLNQPLIIGGGIRSTSQIKQLFDAGANVVV
                     IGNKIEEDIDFLLDLREITQQVK"
     gene            complement(6574..7512)
                     /locus_tag="PKN22_02335"
     CDS             complement(6574..7512)
                     /locus_tag="PKN22_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS41570.1"
                     /translation="MKVLFIDTVHSILQQRLEAKGVFCKDASKLSKEAIQQELNDTDG
                     IVIRSRFRMDAALLDFAPQLQFIARSGAGMENIDCDYCEQRNIQLFNAPEGNCTAVAE
                     HALGMILALFNQLKKGDAEIRKGIWDREGNRGVELTGKTVGIIGYGHNGSAFAKLLSG
                     FNCRVLAYDKYKTGFANGLVEECTLEQLYEQAEIISFHIPQNEETLFWANADFFSKVK
                     HPFYLINLSRGKIVKIEALNKAINDGKVKGAVLDVLEFESASFENIFGNEIPPTFKEL
                     LESDKVLLSPHVGGWTVESYFKLSDVLADKIEAAYF"
     gene            complement(7512..8864)
                     /gene="mgtE"
                     /locus_tag="PKN22_02340"
     CDS             complement(7512..8864)
                     /gene="mgtE"
                     /locus_tag="PKN22_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNS41571.1"
                     /translation="MRFNLTKEFIDQTKAAIAANDSDWLKINVLELHFADIAEVLHPL
                     TNDEAKFVYFLMDEDQQAEVLMELEEDVRDRFLASLSSQEIATQLENLDSDDAADILG
                     ELSDKKVQEVISLMQDDEAADDIVDLLKYKDGTAGSLMQKEFIKARVNWPIDRALLEL
                     RRQAEDVKRVYSIYVVDDADRLLGVLSLKEMLYSKPKTKIADIFQDKNIISVKTNEVT
                     EVVSKTMDKYGLVAVPVVDLQNKLVGRITIDDIVEYIKEEADKDFQLATGISEKVEHT
                     SSVWKISRARLPWLIIGMLGGTMSAHVISGFEEQISKVTALAFFIPLITAMGGNVGVQ
                     SSAIVVQSLAKGSDIFSNIFKKVGKELLVAMLNGILLSTIIYGIGIFFEGSKLAIVVS
                     LSLFTVIIFASIFGVLIPLGLKKLKVDPALAIGPFVTTLNDIVGLFIYFTIGVMLYKF
                     "
     gene            8959..>9289
                     /locus_tag="PKN22_02345"
     CDS             8959..>9289
                     /locus_tag="PKN22_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNS41572.1"
                     /translation="MSRILTGIQSTGTPHLGNLLGAIVPAIEMSNKSENEAFLFIADL
                     HSFTQIKNPTELKENTLSTAATWLSFGVDVHKTVFYRQSQIPEVTELMWYLLCLFPYS
                     RLSLAHGF"
BASE COUNT         3000 a         1834 c         1515 g         2940 t
ORIGIN      
        1 gtaccattat aacagtaacc taaaccacga gaaacgtcac aaccaacata gtcgtcacca
       61 gaataaccta aatctgggtc aacatattga gagaagaatg tatttgtcaa tgtttgagtt
      121 gaacggttaa ttaactcgta gttatagaaa gtcatacggt taatttcgtc agaagtagca
      181 aaagcaaaag cttgtgcacg gatttccata ccaattggtt caccttgaga ttcagtgtga
      241 acatttcctt tatcgttgaa tacaaaccat acagtttggt caccaaataa agttacacga
      301 cggtcaacac cacattgtac atcatctctt cctaagatat cgtcatacca aggataatcc
      361 ccatcatcca ttgggttata aatttggtca gcatttctat cgtagaaagg agccaaataa
      421 taatcttgga ataaacttgg gtctccgtga gctggccatt ctgtgattgc agctaattca
      481 gcgttagttg gcaatgattc ttcaccaatt aaatcacatg ctcctggcgc tttatttggg
      541 tcaatacaag attcccacca aatgctaaag cgaataactg atgctttatt gattgggaag
      601 aatttatcgt attttgcaca ctggtcaccc gaaatattac catcaccata aggacgaatt
      661 gtattatctc ctacaggatt caacgggttg tagtttacat ttggagcata aggaatggtt
      721 acagttaaag gaccacccca aaagtcgtta ccaacttgac ggaaacgtac agctgctaat
      781 ttcaactgac cgttttggtc acgtccaccc atccaaagag cagctgcgaa aattgctttt
      841 gcaccaccac ctttaggaac ttcatatcct gctacacttt tttgttcgtc ttcaaacaaa
      901 cgtccaccca tttgtaacaa tgctgaaacg tcgttgaaat caaggatata cttttttaca
      961 gcaggagcac atgcagcgcc tttttcactg ttagaaggtt ttaccttgtc gttaccttta
     1021 ggttgaacat tactgcttcc tgtgtacaaa tacgccaggg acatattaga gaacaaggta
     1081 aaagttccca atactaaagc agattttatt attcttttca tatcttcttt gttagtatga
     1141 attaaaagtc gaatttaata cccaaacgaa tagttcttgg tgcactaatg ttatatgggt
     1201 tttgaatctt catcaagtag taatttctaa atgaagcttc gtcagtttga ttttgaatag
     1261 aagtttggct gcttgctgct tctaagtaac catcatcatt ccagttacct gttgcgcggt
     1321 aaacagacaa gatgttcttt tggttcaaca agtttgtaac acgtaaatat acatttaaat
     1381 atgtcgtacg ttttttgtct tcttttttac ctacttcaaa ctcaaatgtt ctatccaatt
     1441 ggatatccaa tctgtaagac caaggtaaac gtgaaccgtt agttgttcca tccaaaccag
     1501 aagcttgagg cgagaacata ccttcattta cgatatctaa ttttttagaa tatgggttac
     1561 cagaatagat gttagccata atgttcaatc ccatatttcc aaatacttcg tagtttttaa
     1621 tacgtggacc gttataatct ttacctgtac cgtatctata gtcaaaagag atagcaaacg
     1681 catgacgttg gtcaaatgag taagggaaga ttgcacgtaa gttaggtaaa cctgcgttaa
     1741 tcaaacctgc cattgaagtt gcatcagaac cagtaccatc agcaaactgt aaggtatatg
     1801 ctgcagtcat acggatattt cctgttcttc ttaagtcata agcaacagtc aaacctttta
     1861 ctgtaccaaa gtcgatgtta ccaaaagttt tgtaagttgc tggataagct tcaacaacgt
     1921 taatcaactg tacgttatct ctttgttcac gatagaaagc agagattttc aaagaagaag
     1981 ttcttgtcaa cacttgttgg aaacctaatt cgtaatcgat tgttttttca ggcttcaagt
     2041 tagagttgtt aataatctca cttcttgttt gaatgaattg gtattcataa ggatcaaaac
     2101 gtacacctgt tgtagggcgt tttgttaaga tgtcatagtg agcaaagaat gaagattctt
     2161 cagacactgg gaaagagaat gcgatacgag gcattacgct cacttgtgct ttgtaatctt
     2221 taaatgcgct acttgaaatt gtagtttttc cagcatctgc taaccaagga gcgataccgt
     2281 ttgcaccacg caatacagca gggtcttcaa cttgatcacc atttgcattg taccaaacat
     2341 caccatttct aaatccattg atttgagttg gtgcagcaac atcatcaaca tatactgtat
     2401 agtcatctcc gatcgaagta ggtacattta cccagctata atcagtactg ttcattttag
     2461 ctcttgcttc acctgcagtt aatgctgaat ggaataaata tttatccttc aaaactggtt
     2521 ggttagcatc aaatacgtca acacgaaccc caacgttgaa tactaagtca cggaatgcaa
     2581 atttatccat gatgtatcca gaaatataag ttggagagaa tgctcctaca aaacgtttgt
     2641 agttaccatt ttcatcaaac tcattgaaat atttatcaat atctgttctt ccttttactt
     2701 tatttcctgc atggtcataa ccataataag aaacataaga ctttccgtta ttcaacaatt
     2761 cgtctggtgc aaacattcca aaattcaaac gacttggatc cattgcgtca atttgaatgt
     2821 agtcattacc acctggatct aaacctaatt ctttacgaac gttgtagtca aagaatgatt
     2881 ggtaatctac ttgaccacca gaagcaccac tttgtgcacc tccaaattga ccattgtgat
     2941 tatgtgcata acccgtattc aaacggttgt aatagatttt ttggaaacct gcttcttgag
     3001 agatagtagg atttgggtcg tttttatcga tctcagaaat atggctatta gctaacaaac
     3061 gagccaacga ccacaatccg ataggtccgt tatcaccaga gctccatcct ctatccgttc
     3121 tttgttcgta ctcaaatcct aaagagatag agtggtcacc aacgttgata gatcctgaac
     3181 cagtaacacg taactgattg ttttctgaaa ttccgaaacc attataaggt gttccgatgt
     3241 tgttccaaat ttggtaaact gaaattggcg catctccatt caacaaggca tttccttgtc
     3301 ttaattgatt gaagttagcc gcagaaccaa ataaatcata gtattggctt gttaacgccg
     3361 ttaaaccagg gttggtttca gaacctgtaa atgtaacacc attttggaat ggcacctctt
     3421 ggtttacata agcattcaag ctatcaacaa aaacgaaaga tgtatcaaaa cgagatgtaa
     3481 atgttccaac atgtccatag ttaaacatat tgaaaccatg ttttgggtca tacgattttg
     3541 aacgtgattg agagtagtca aacatcaacg agtaaaaagc agatttcact tttgatgcag
     3601 aaccttctct tttatttgag aaacgttgag acaaacgtcc aaacaccctc cagtctaagt
     3661 ttttacctct accaaagttt tggaagttca acaatgaact agttctatcg tataaacttc
     3721 tatcatagta gttcatagac ccaccgaaag acaagtttac agatggccct gtatttacgt
     3781 caatttttgc ttgtgcagat acgttttggc ttcttgcatt cattctccac tttgtttttt
     3841 cgaaatcgtt ttcttttaac aaagtagcgc gatagaatgt accatcacca tttgcattca
     3901 tcactaaagg atattgcaac aatgaatctc taatttcttt tttaacacgg tagctacctc
     3961 catttaaagg acggttatcc aattcattca agtagtttgc agataacaag aatcccataa
     4021 taggttttgt ttttgcacct gttgaatctt ttttcatgaa taaaggacca gagatcatcc
     4081 cttcaactaa gttgtaggca tatttatcca aaccaaaaac ttttccgtca taaccgtctt
     4141 tgtctttacc tttaatgtaa aaaccagaag atacagcctc gatacttcca aagaattttg
     4201 aagaaggtcc tcttgttgtt acagagataa ccccaccggt aacgtcacca tagttagcag
     4261 gtaaaccacc tgtgattacc gatacctctt cgattgctga ttttggcaag ttggaagatc
     4321 ctcgtacttt aataccgtca atatagtaat acgtagcatc tgaacgagaa ccacgaacac
     4381 taattgcacc acttccttca tctgtattta caccaccaac tgtttgtgca acaccagcag
     4441 cagaacgaac tggtaaacga gaaatatcct cagcagtaat tgttgaacct gatgcaccac
     4501 catctttatt aataagtggt actttgtagg caactactac taattcttcg atgttttgaa
     4561 catctttagg cttagatatt tccgtattat ccaagaaagt aattttatca gcatttacaa
     4621 ttacaccacg aattgcagta ggctgatatc catctccctc gtttcttact tccacatcgt
     4681 aagaacctgg tgcaatcgag ttgatttgga attttccatc atcatccgta ttggcacccc
     4741 cttttaccgt tccatcttgc aagagtaaca cttttgcaaa accaattggc cctcttgttt
     4801 caccatcaat aacagaccct ctaatagttc ccaaacctga ttgagaaaaa gagtatgcta
     4861 ctgataataa aacacttaaa attactaaat gtaaattttt caacataagc acagattatt
     4921 atttaactta ttatctacaa aacaaagcgg taaatatata aatattaatt cgacatatac
     4981 ctcttcattc caaaaaaatt aattttttgt taaattaact ttccttaaca aatgtagaat
     5041 aaaagttttg aaataaaaaa atacacttct ttaaacactt gttttttagc gcaatttagc
     5101 ctactttaat ttaaaaatac cttgctttaa aaattaattt aaaacagctc actaaaaaaa
     5161 atatacattt aaagtatttt ttaattttta aagaaaaaaa ttagttttac aaacgtgttt
     5221 tatttaaaaa atataaaaat tggtgacagc accctgtatt tcattcgttt cgatgagtac
     5281 aacatcaatg attacaccta tttattaaca aatgatgagc aatttaaact ttccactttt
     5341 aaaagtgaga aaagaaaaaa ggaatttgtt gcgacaagga ttttaaaaac tacactattt
     5401 aaaaatactc caatttatta tttatccaca ggagctcctt attttaaaaa ttcgaccaat
     5461 catatttcaa ttagccactg cgataattgg gttgtatttg ctgtttgcaa ctgctataaa
     5521 attggagtag atattgaacc tataagcagt aaagcacaac gactacacca aaaatttttg
     5581 aacgaaagtg aaattgcact tttagacaca tccaacattg aacttatgtc aaaagcatgg
     5641 tcgtgcaaag aagcactcta taaattaagc actaaagaag gaattatttt taaaaaagac
     5701 ctcttaataa cagattttga taaaaaaaac acattcaact gtaaggtttt agaagaaaac
     5761 aaaagttttt ctgtacattt gagtgcaagt atcattgatg gatacattgt tacaataaat
     5821 acatcgccac tttatgaaaa tcattgattc tattattgac caacaaaaaa ttgctgtttt
     5881 gattgaccct gaaaaatgtc aatccatagc atatatcgag caattaattg aaaaaattca
     5941 ttttagtggt gttgatttta tatttattgg aggcagcacg gtgagcgaaa aagaatttaa
     6001 ccaaacgata gctgcattaa aaaaattagc acgaatccca ataattattt ttccaggttc
     6061 ttctagtcag gtatcaccat tggcagatgc tatcttgttg ttaaacttaa tttctggtag
     6121 aaatgctgat tttttgatta cccatcacgt gcaagcagca caaaaaattg ttgacgctgg
     6181 attggaagtt atttcaacct cttatttatt gattgatggc ggcagtcatt catcagttgc
     6241 ttatgttagc cagacgacac caattcctca acaccaacta tctattgcta cgcaaacagc
     6301 tttggcagga aaattaattg gccaccaact ggtatttatg gatgctggaa gtggtgcaaa
     6361 aaattgcgta tcaacagaaa tggtaaaagc agtaaaacaa ttgaatcaac cgttaattat
     6421 tgggggtggt atccgttcaa ccagtcaaat caaacaactt tttgatgcag gcgccaacgt
     6481 ggtggtaatt ggtaataaaa tagaagaaga cattgacttt ttattggatc ttcgagaaat
     6541 tacacaacaa gtcaagtgat agaaattgga tgattaaaaa taagccgctt ctattttatc
     6601 ggccaacaca tcgcttaatt tgaaataact ttcaactgtc catccaccca catgtggcga
     6661 caatagcact ttatcacttt caagcaattc tttaaaagta ggtggaattt cgtttccaaa
     6721 aatattttca aaacttgcgc tttcaaattc caatacgtcc aaaacagccc cttttacttt
     6781 tccatcgttg atcgctttgt taagcgcctc aatttttaca atttttcctc ttgataagtt
     6841 aattaagtaa aacggatgtt tcacttttga aaaaaagtct gcatttgccc aaaaaagtgt
     6901 ttcttcgttt tgaggtatgt gaaaactaat gatttctgct tgctcgtata attgttcgag
     6961 agtacattct tctaccaatc cattagcaaa tcctgttttg tatttatcat aagcaagcac
     7021 acggcaattg aatccagata ggagttttgc aaaagcactt ccattatgtc cgtagccaat
     7081 aattccgacc gttttaccgg ttaattcaac gcctctattg ccttccctat cccaaattcc
     7141 ttttctaatt tcagcatccc ctttttttaa ttgattgaag agtgctaaaa tcatccccaa
     7201 ggcatgttcg gcaacggcgg tgcaatttcc ttcaggcgcg ttgaacaatt ggatgtttct
     7261 ttgttcacaa tagtcgcaat caatattttc cattcctgcg cctgaacgag caataaattg
     7321 tagttgtgga gcaaaatcca acaaagcggc atccatgcga aaacgggaac gaatcacaat
     7381 accatccgta tcgtttaatt cttgttgaat tgcttcttta cttagtttac tagcatcttt
     7441 gcaaaaaaca ccttttgcct ctaatcgctg ttggagaatc gaatgaaccg tgtcgataaa
     7501 taaaactttc attagaattt gtataacatc acaccgatgg taaagtagat aaacaaccct
     7561 actatgtcgt taagtgtagt aacaaacggg ccgatagcca atgcaggatc gacttttaat
     7621 ttttttaacc caagtggaat gagcactcca aaaatagaag caaaaattat cacagtaaac
     7681 aatgaaagac tgacaacaat ggctagtttg gatccttcga aaaaaattcc gataccataa
     7741 ataatagtgg aaagcaaaat accgtttagc atcgccacca acaattcttt tcctactttt
     7801 ttaaagatat tggaaaatat atcgcttcct tttgccagag attgcaccac aattgccgat
     7861 gattgcacac caacgtttcc acccattgca gtaattaaag gaataaaaaa agcaagtgct
     7921 gtaactttac taatttgctc ttcgaaacca gaaattacgt gtgcactcat ggttccgccc
     7981 aacataccaa taatcaacca tggcaaacga gcgcgcgaga ttttccaaac ggacgaggta
     8041 tgctctactt tttcagagat accagttgcc aattgaaaat ctttatcggc ttcttctttg
     8101 atatattcaa cgatatcgtc aatggtaatg cgccccacca atttattttg caaatcaaca
     8161 actggaacag caaccaatcc atatttatcc atagttttag acaccacttc cgtaacttca
     8221 ttggttttta ccgaaatgat gtttttatct tgaaaaatat ctgcgatttt agtttttggt
     8281 ttggaataca gcatttcttt gagcgacaaa acaccgagta atcgatcggc atcgtccacc
     8341 acataaatgg aatatacgcg tttaacatct tcggcttgac gacgcaattc caaaagtgct
     8401 ctatcaattg gccaattgac acgtgctttg ataaactctt tttgcatcaa actaccagca
     8461 gttccgtctt tgtatttcaa caaatcaacg atgtcgtcgg cagcttcatc gtcttgcatt
     8521 aaagagatga cttcttgcac tttcttgtcc gacaactcac ctaaaatgtc agcagcatca
     8581 tcggaatcaa ggttttctaa ttgtgtggca atttcttgag atgacaacga tgctaagaaa
     8641 cgatcgcgca catcttcttc gagttccatc aatacctcgg cttgctgatc ttcgtccatt
     8701 aaaaaataaa cgaattttgc ctcatcgttg gtaagtggat gcaacacctc tgcaatatct
     8761 gcaaagtgca attccaatac attgattttt aaccaatcag aatcattggc cgcaattgca
     8821 gcttttgttt gatcaataaa ttctttggtt aaattaaatc tcatagaaat tgaatttgca
     8881 ccacaaattt agggttcttt atcaaaagaa tcaaagtatt taaaaaaatt aataattatc
     8941 ttcgcaaaaa atctaaatat gtcgcgaatt cttacaggta tacaaagtac tggaacacca
     9001 catttgggaa atttgttggg agcaattgtt cctgccattg aaatgtcgaa caaatcagaa
     9061 aacgaagcgt ttttgtttat tgctgatttg cattcattta cacaaattaa aaacccaaca
     9121 gaattaaaag agaacacact ttcaacagct gcgacatggc ttagttttgg tgtagacgtg
     9181 cataaaacag tattttacag acaaagtcag atacctgaag ttaccgagtt gatgtggtat
     9241 ctattgtgtt tattcccata ttctcgtttg agtttggcac atggattta
//
LOCUS       DAOQVQ010000041         9176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8422_length_9176_cov_3.608047, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000041 DAOQVQ010000000
VERSION     DAOQVQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8422_length_9176_cov_3.608047"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKN22_02350"
     CDS             complement(<1..602)
                     /locus_tag="PKN22_02350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS41573.1"
                     /translation="MKTLAVAIITLNEERNIRRCLTSIRDLADEIIILDAFSSDKTVA
                     IAQEFNARVEQRTWQGYAASKNYLNSLVQSDYILSLDADEALSEELYQTLKREKEKGF
                     SETYSVNRMTNYMGKWIKHSGWFPDIKLRIFPKEGSYWTGEFVHEELITNDKTPQLLK
                     GILEHYSYYSYEDHQQRADKYSRLTAQKMHVAGKKANLLKP"
     gene            complement(609..2129)
                     /locus_tag="PKN22_02355"
     CDS             complement(609..2129)
                     /locus_tag="PKN22_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNS41574.1"
                     /translation="MMRLFVATFFTLFCAIVVAQPQNNAVKTTDDPFQEVVDQSLAMF
                     YAEYAKKQNQSYEAVIDSLNYADGQVVSFSDEVYCQRLNEINKTTPFQLDCNSVSLST
                     IRFFVANRRGFARVALGRSKLYFDLFEKKLQEYGLPLELKYLSVIESGLRPQVKSPAG
                     ALGLWQFMYGTGKIFGLKENYYFDERMDPEKSTDAACRYLKQLYNIYGDWNLALAAYN
                     AGPGNVNKAIRRSGNKTTYWAVRPFLPRETQGYVPNFIAAVYLLTYHAEHNIIPAEAA
                     VHYAELDTMCLKSGIHMSTIEKLVGWDEVDIKAVNPVYKYNYIPETTPKQCISGPIKY
                     IGKLVSMEDSLYSLEKSIFSPQKVTSPVATNSTTDSTDVDAKDSTNTAILPGTTEAGN
                     TFYHKVEQGENLNTIATKYGTTINKIMAINGLSTTKIYVGQRLKIESKTGGDTPTVAP
                     SPTPSPVAKPQYYSVRSGDTFGKIAQRYGKSISQLKALNPRVNIERLEVGQRIRVK"
     gene            complement(2136..3539)
                     /gene="gatA"
                     /locus_tag="PKN22_02360"
     CDS             complement(2136..3539)
                     /gene="gatA"
                     /locus_tag="PKN22_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNS41575.1"
                     /translation="MYRTIDEVKAQLATGKTVVSIVNDYLLEIEKKKELNAFVEVFTN
                     SALEQAKLVDEKLANGTAGKLAGVVVGIKDNLCYTGHKVSASSKILDNFESLFTGTAL
                     QRLIDEDAIIIGRLNCDEFAMGSSNETSYHGVVKNPLDTTKVPGGSSGGSAAAVAAGL
                     CTVSLGSDTGGSIRQPASFTGTYGLKPTYGRISRYGLIAYASSFDQIGPFANSTKDIA
                     LLLQIMAGKDGYDSTASSKEVPNYLDGQLASKLKIAVLKESLETDGMDAEIVAKMKAL
                     IAQLEAKGHEVELVSFPYLKYVVPTYYVLTTAEASSNLSRYDGVHFGYRSATASGVEE
                     TYKKSRSEGFGKEVKRRIMAGTFVLSHGYYDAYYTKAQKVRRVLKDKTNEIFNDFDFI
                     VLPTTPSTAFELNGVKDPIQMYLQDIFTVHANLTGHPAISLPLGNHSNGLPFGVQIMG
                     KAFDENGLLNFAQYLEN"
     gene            complement(3555..3746)
                     /gene="tatA"
                     /locus_tag="PKN22_02365"
     CDS             complement(3555..3746)
                     /gene="tatA"
                     /locus_tag="PKN22_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNS41576.1"
                     /translation="MAGAVLGVVGPWQIVLIVVVLLLMFGGKKIPELMKGLGQGVKEF
                     KDATKGNDEKEVKKEEESK"
     gene            complement(3865..4239)
                     /locus_tag="PKN22_02370"
     CDS             complement(3865..4239)
                     /locus_tag="PKN22_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNS41577.1"
                     /translation="MSARVDKFVWCVRLSKTRSIATDLVSKGKVKINGEPCKPSKEVK
                     IGDVVSLTKNNATFSYKVIQLLNNRVGAKLVTDYILDITDPLEIEKYKAYQAAQSTYR
                     QLGTGKPTKKDRRDISDFLNDW"
     gene            complement(4302..4652)
                     /locus_tag="PKN22_02375"
     CDS             complement(4302..4652)
                     /locus_tag="PKN22_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNS41578.1"
                     /translation="MDLETLRNYCLAKKGTTECFPFDATTLVFKVGGKMFALIDVEEA
                     SSINLKCEPEKAIELREEWEEITPGFHMNKIHWNTVSLIGRLSDRLVFELIDHSYELV
                     FLSLTKKIQNEING"
     gene            complement(4648..5775)
                     /locus_tag="PKN22_02380"
     CDS             complement(4648..5775)
                     /locus_tag="PKN22_02380"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNS41579.1"
                     /translation="MNINSVKYTQHHNSEFYKELKKRVNAYFKTNNISKYGNTGMVVK
                     SIFMILLYFVPLILILTVVEQSWLSLLLWVVAGFGMAGIGLSIMHDANHNAYSKHLFV
                     NKFFGFLVNFVGGNDLNWRIQHNVLHHTYPNVAGVDEDIDVDGLMRFSPNQERLKFHK
                     YQHLYAWFLYGLLTINWFLRKDYQQIVRYNKMGLLTTQSITFKKAISLVIFTKIIYMF
                     LTIALPIWLAPTPWYISVIGFVIMQFVAGFLLSIIFQSAHVLPTSEFPVPDESGNIES
                     DWAINQLYNTANFAPKAKLLSWFVGGLNYQVEHHLFPNICHVHYKNISKIVKETAIEY
                     NLPYYSYATFFGAIKDHAKLLKRLGKHDVLAPVAVKSNNEH"
     gene            5925..6497
                     /locus_tag="PKN22_02385"
     CDS             5925..6497
                     /locus_tag="PKN22_02385"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNS41580.1"
                     /translation="MKKVKLALFASGTGSNAINIIRYFKNDATVEVGFVLCNKPEAKI
                     VTSAQDENIPVVLVSNQEVEEGQKIVELCQKEAIDYIILAGFLRKIPVALIEKYPHHI
                     INIHPSLLPKYGGKGMYGANVHKEVLKNNESESGITIHVVNAEFDKGEILAQFKCTLS
                     ENETLESLQQKIHQLEHTNFPQVIENTIKK"
     gene            6494..7090
                     /locus_tag="PKN22_02390"
     CDS             6494..7090
                     /locus_tag="PKN22_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNS41581.1"
                     /translation="MTELIDSFSWSQVFRATMVLFAVIDIIGSIPLIIKVQEKSGYVS
                     PMRTSIVSFGIMLGFLILGESILNIFGVDIRSFAVAGSIIIFALALEMIMGWDLFRDE
                     ASSKVASVIPLAFPIIAGAGTLTTLVALKAEYHEINILVAIVINVVIIFTVLKLTKKI
                     EHILGEGGIGIVKKVFGIILLAIALKLFSSNITFLFAK"
     gene            7213..7875
                     /locus_tag="PKN22_02395"
     CDS             7213..7875
                     /locus_tag="PKN22_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNS41582.1"
                     /translation="MDSIGKITEDIINRSPFLRESMTEGLINISALARKIQPEIETVV
                     GKKVKEGAIVMAIKRMSPGLYHKLNIKIKNVLGELGDFLVRSNLSDFTYHNSESLGMK
                     QSELMRQLNNEPDAFFAFVRGVTETTVITSDKYNDLIEKLFANEKLITSTQDLASITV
                     KLPRINIEIYGIYYYILKQLAWEGINIIDVISTANEFTVLVSQDDVDKAFKTLMQLNR
                     GF"
     gene            complement(7872..>9176)
                     /locus_tag="PKN22_02400"
     CDS             complement(7872..>9176)
                     /locus_tag="PKN22_02400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS41583.1"
                     /translation="SFSSFSGEELKQRVEQLLTEQANEHQLVAQTILQQVKQQLDEYS
                     KLLNTPAQRLTAEEKMLKQQLKKGKALLVKEFAKSRQHPTMRELLQSEANLWIKALKP
                     VWLTNPTSLAKAFPLQTNLFDMCIVDEASQMPVQNGLGALHRCQYAIIAGDEQQMSPT
                     NYFQAGAQEVVSLLHHASYYFANHTLTHHYRSKHAQLIAFSNEHFYNNQLLAYPSYPV
                     DNNCIQRYFCQQGRFVNRKNEHEANKVVELLDKHLPTNKTIGVVAFSAEQIDAIFKAI
                     PQNKLALVQQKIDENTLFFKPLEKVQGDECEVLIISLGYAPNEEGQFSWHFGPLNTQS
                     GRNRLNVLLTRASEKIEFVCSIEAQSVQWSDNESIQLLYKWLYQLENLPNASTLEFPQ
                     NLEPQISGNQLNITHSFAKLKHALELTTTYAVLSKRGWNVNF"
BASE COUNT         2830 a         1738 c         1538 g         3070 t
ORIGIN      
        1 ggttttaata agttagcctt tttaccagca acatgcattt tctgagcagt taaacgcgag
       61 tatttatctg cacgctgttg gtgatcttca taagagtaat aagaataatg ttctaatatt
      121 cctttcagta attgtggggt tttatcgtta gttataagtt cttcgtgtac aaattctcct
      181 gtccaataag aaccttcttt tgggaagata cgtagtttaa tgtcgggaaa ccatccgctg
      241 tgtttaatcc acttgcccat gtaattggtc attcggttta cagagtacgt ttcactaaaa
      301 cctttctctt tttcacgttt aagcgtttgg tataattcct cactgagagc ttcatctgca
      361 tccaatgaaa ggatgtaatc cgattgcact aaactattga ggtaattctt acttgctgca
      421 tatccctgcc aagtgcgttg ttctactcga gcgttgaatt cttgggcaat cgcaaccgtt
      481 ttatccgaag aaaaagcatc caaaatgatt atttcatcag ctaagtcgcg aatggaagtc
      541 aaacaacgcc gaatgtttct ttcttcattg agtgttatta tcgcaactgc taaagttttc
      601 atggcaattt attttacacg tattcgctgc cctacttcta atcgttcaat attaacacga
      661 ggatttaaag cttttaactg actaatggat ttcccatagc gttgtgcaat ttttccaaaa
      721 gtatcacccg aacgcaccga ataatattga ggttttgcaa ccggagatgg tgttggagat
      781 ggtgctactg ttggagtatc tcctcctgtt tttgattcaa tttttaagcg ttgccctaca
      841 taaatctttg tagtgcttaa tccattaatt gccattattt tgttgattgt tgtgccgtat
      901 ttggttgcaa tcgtatttaa attttctcct tgttcaactt tgtgatagaa cgtgttgcca
      961 gcttctgtag ttcctggtaa aatagccgtg tttgtactat cctttgcatc cacatctgtt
     1021 gaatctgtag ttgaattggt tgcaacaggt gacgtcactt tttgtgggct aaaaatactt
     1081 ttttctaagc tatacaaaga atcttccatg ctaaccaatt tgccaatata tttgataggg
     1141 ccagaaatac attgttttgg agttgtttct ggaatataat tgtatttata cactggattt
     1201 actgctttga tatcaacttc gtcccagcct actaattttt caatcgttga catatgaatg
     1261 ccgcttttta aacacattgt atcaagctca gcataatgta cagccgcttc agcaggaatg
     1321 atattgtgtt ctgcatgata agtcaacaaa taaacagctg caataaaatt gggaacataa
     1381 ccttgtgttt ctctaggtaa gaaaggacga actgcccaat aagttgtttt gttacccgaa
     1441 cgacgaatgg ctttgtttac gttacccggt ccagcattgt aagctgctaa tgccaaattc
     1501 caatcgccat aaatgttata caattgttta agatagcgac aagcagcatc cgttgatttt
     1561 tctggatcca tgcgctcatc aaagtaataa ttttctttta acccaaagat tttacccgtt
     1621 ccatacatga attgccataa acctaatgca ccagctggag atttcacttg cgggcgcaat
     1681 ccactttcaa taacactcaa atattttaat tctaatggaa gtccgtattc ttgtaatttt
     1741 ttctcaaata agtcaaaata caatttagaa cgacccaatg ctacacgcgc aaaaccacgg
     1801 cgattggcta caaaaaatcg aatggttgaa agcgacaccg aattacaatc caattgaaac
     1861 ggcgttgttt tattgatttc atttaaacgc tgacagtaaa cttcgtctga aaatgaaact
     1921 acttgaccat ccgcatagtt caacgaatca attactgctt cataactttg attttgtttt
     1981 tttgcatatt ctgcataaaa catggctaac gattgatcaa ccacctcttg aaatggatca
     2041 tctgttgttt taacagcatt gttttgcggt tgcgcgacta caatcgcaca aaataatgta
     2101 aaaaatgttg ctacaaacaa cctcatcatg ttctattaat tttctaaata ttgtgcaaaa
     2161 ttcaacaagc cattttcatc aaatgctttt cccataattt gaacaccaaa aggcaacccg
     2221 ttagagtggt ttccaagcgg taatgaaatt gctgggtgac cagttaaatt tgcatgaact
     2281 gtaaaaatat cttgtaaata catttgaatg gggtctttaa caccgtttaa ttcaaatgct
     2341 gtggaaggag ttgtgggtaa cacaatgaaa tcaaaatcat tgaaaatttc attggtttta
     2401 tctttcaaca cccttcttac tttttgtgct ttggtataat aagcatcgta ataaccatgt
     2461 gacaaaacaa atgttcccgc catgattcgt cgttttactt ctttaccaaa accttcactg
     2521 cgtgattttt tataggtttc ttccacccca cttgctgttg cacttctgta tccaaagtga
     2581 acaccatcat aacgcgacaa gttagacgat gcttctgcag ttgttaacac ataatacgta
     2641 ggaactacat actttaaata aggaaaagac accaactcaa cctcatggcc ttttgcttct
     2701 aattgtgcaa tcaatgcttt catttttgca acaatttctg catccattcc gtctgtttcc
     2761 aaactttctt tcaataccgc aatttttaac ttacttgcca attgaccatc caaatagttt
     2821 ggaacttctt ttgatgaagc ggttgaatca tagccatctt ttccagccat tatttgcaaa
     2881 agtaaggcaa tgtctttggt agaattggca aatggaccaa tttgatcaaa cgaagaagcg
     2941 taggctatta aaccatagcg actaatgcga ccataggttg gttttaaacc gtatgttcca
     3001 gtaaaagatg ccggctgacg aatagatcct cctgtgtcac ttcctaatga aactgtacac
     3061 aaacctgcgg caactgctgc ggccgaacca cctgaagaac cacccggaac tttggtagta
     3121 tctaatggat ttttaacaac accatgatac gaagtttcat tggagcttcc cattgcaaat
     3181 tcatcgcaat ttaagcgacc aatgataatt gcatcttcat caattaagcg ttgcaaagca
     3241 gtacctgtaa acaatgattc aaagttgtct aaaatttttg atgatgcact aactttatga
     3301 cctgtgtagc acaaattatc tttgattcct acaacaacac ctgccaattt cccagcagtt
     3361 ccatttgcta atttttcatc cactaatttt gcctgttcca atgcagaatt agtaaaaact
     3421 tcgacaaaag cattcaactc cttttttttc tctatttcca ataaataatc attgacaata
     3481 gaaacaacag ttttccctgt cgcaagttgc gcttttacct catcaattgt acgatacatg
     3541 ctaatggtat agaattattt gctttcttct tcttttttaa cttctttttc gtcgtttcct
     3601 tttgtagcgt ctttgaattc tttaactcct tgacctaacc ctttcattaa ttctggaatt
     3661 ttcttaccac caaacatcaa taaaagtact acaacgatta atacaatttg ccatgggcct
     3721 actaccccta atactgctcc tgccataaaa cttagtttta attttaatac aagctacaaa
     3781 tgtaatgaat tatcgaataa catattcact tttcggaaag aagtcttgaa gtaagttatc
     3841 attttttaac agaatgatga caaattacca atcatttaaa aaatcagaaa tatctcttct
     3901 atcttttttg gttggttttc ctgttcctaa ttgacggtaa gtgctttgcg ctgcttggta
     3961 ggctttgtat ttttcaattt ctagtggatc ggtaatatcc aaaatgtaat ctgtaaccaa
     4021 ttttgctcct actcggttgt tgagtaactg tatcaccttg tatgaaaatg ttgcattgtt
     4081 tttagtgagc gaaaccacat ctccaatttt tacttctttg gacggtttac aaggttctcc
     4141 attaattttt accttgcctt ttgaaactaa atcagtagca atacttcttg ttttggacaa
     4201 acgcacacac cacacaaatt tatcaactct ggcactcata ttattgcatt tttgaaaggc
     4261 ctaaaattaa ataatgagga tgactattca aagaaaaaaa atcaaccgtt gatttcgttt
     4321 tgaatttttt tagtcaacga taaaaacacc aattcatacg aatgatctat taattcaaac
     4381 accaacctgt cggataatcg accaatgagt gaaacggtgt tccaatgtat tttattcatg
     4441 tgaaaaccag gcgttatttc ctcccattct tcgcgcaatt caattgcttt ttctggttca
     4501 cattttaaat taatactcga tgcttcttcc acatcaatca atgcaaacat ttttccacct
     4561 actttaaaaa ccaaggttgt tgcatcaaaa ggaaaacatt cagttgttcc ttttttggct
     4621 aaacaataat ttcgtagtgt ttctaaatca atgctcattg ttggatttta cagcaactgg
     4681 agccaataca tcatgtttac ccaagcgttt caataatttt gcatggtctt ttattgcacc
     4741 aaaaaatgta gcataactgt aatacggcaa gttgtattcg atggctgttt ctttcacaat
     4801 ttttgaaatg tttttgtagt gcacatgaca aatattgggg aataaatggt gctcaacttg
     4861 gtagtttaat cctcccacaa accacgacaa taatttggct tttggtgcaa aattagcggt
     4921 gttgtacaat tgattaattg cccaatccga ttcaatattt cctgattcat cgggtactgg
     4981 aaattcagac gttggcaaca cgtgtgctga ttggaaaata atagacaata aaaaacctgc
     5041 aacaaattgc ataattacaa aaccaattac agaaatgtac catggcgttg gcgccaacca
     5101 aatcggcaaa gcaatggtta aaaacatgta tataatcttt gtgaagatta ccaaagaaat
     5161 ggctttttta aaggtaatgc tttgtgtagt taacaagccc attttgttgt agcgtacaat
     5221 ttgttggtaa tctttacgaa gaaaccaatt gatggtcaac aaaccatata aaaaccacgc
     5281 atataaatgt tggtatttat gaaatttcaa acgttcttga ttgggtgaaa aacgcatcaa
     5341 tccatccaca tcaatgtctt cgtcaacacc tgctacattg ggataggtgt gatgtaaaac
     5401 attgtgctga atgcgccaat tcaaatcatt tcctccaaca aaattgacta aaaatccaaa
     5461 aaatttattg acaaacaagt gctttgaata ggcattatga ttggcatcgt gcatgataga
     5521 caacccaatt ccagccatac cgaaaccagc aaccacccac aataacaagc tcaaccacga
     5581 ttgttccaca acagttagaa ttaatattag aggaacaaaa tacagcaaaa tcataaaaat
     5641 ggattttact accattccag tattaccata tttggagatg ttgtttgttt tgaaataagc
     5701 attcacacgt tttttcaatt ctttataaaa ttcggagttg tgatgctgcg tatatttaac
     5761 ggagtttatg ttcattttct gattaaaaag tggcgcaaga tacttatttt tgatggaacc
     5821 cattatgatt ttaatcacct tgaataaaat taacatttat tagtaatcaa aaaatcttat
     5881 tcatcattaa caatttcacc gatgtatttt tataattttg caccatgaaa aaagtcaaat
     5941 tagcactatt tgcctctgga acaggtagta acgccatcaa tattattcgc tattttaaaa
     6001 acgacgctac tgtagaagtg ggatttgttt tatgcaataa acccgaagcc aaaattgtca
     6061 cttcagctca agatgaaaac attccagtag ttttagtttc caatcaagaa gttgaggaag
     6121 gacaaaaaat tgttgagttg tgtcaaaaag aagctattga ctacatcatt ttagctggtt
     6181 ttttacgtaa aattcctgtt gcactgattg aaaaatatcc gcatcacatc attaacattc
     6241 acccatcgtt gttgcctaaa tacggtggca agggcatgta tggtgcaaat gtgcacaaag
     6301 aagttttaaa aaataacgaa tcagaatcgg gtattaccat tcacgttgtt aatgcagaat
     6361 ttgataaagg tgagatttta gcacaattta agtgcacact ttctgaaaac gaaacattag
     6421 aaagcttgca acaaaaaatc caccaattag aacatactaa ttttccacaa gtaattgaaa
     6481 atacaataaa aaaatgacag agttaattga ttcttttagt tggtcgcaag tgtttagagc
     6541 aaccatggtt ttatttgctg taattgacat cattgggtca attccactca tcattaaagt
     6601 gcaagaaaaa tcgggctatg ttagtccaat gcgcacaagt attgtatcct ttggcatcat
     6661 gttaggcttt ttaattttag gagaatccat tttaaatatt tttggtgtcg acattcgttc
     6721 ttttgccgtt gcgggctcca tcatcatctt tgctttggca cttgaaatga ttatgggatg
     6781 ggatttattt agagacgaag cgagtagtaa agttgcaagc gttattcccc tagcatttcc
     6841 aatcattgca ggcgctggaa cactcacaac cttagttgca ctgaaagcag aataccacga
     6901 aatcaatatt ttagtggcaa tcgttattaa tgtggtcatt attttcactg tcctcaaatt
     6961 aacaaagaaa attgaacaca ttttaggtga aggtggtatt ggcattgtga aaaaagtatt
     7021 tggaatcatc ttgcttgcca ttgctttaaa attgttttcg tccaacatta ccttcttgtt
     7081 tgcgaagtaa ctttttgtta caattagcgt gaaataattc aatattaaaa ctttttaata
     7141 cattttttta tccttttgtt attattttaa agatattgtt atatttgtgt aacgaaaatg
     7201 aaattttaac ttatggattc aattggaaaa attaccgaag acattatcaa cagaagtccg
     7261 ttcttaaggg aatcgatgac tgaaggccta atcaacatct ctgctttagc tagaaaaatt
     7321 caaccagaaa tagaaactgt tgttggaaaa aaagtaaaag aaggtgcaat tgtgatggca
     7381 atcaaacgca tgtcacccgg tttataccac aagttaaata ttaaaattaa aaacgtattg
     7441 ggtgaattgg gcgacttctt agtacgcagt aatttatctg atttcactta tcacaattct
     7501 gaatcgttgg gaatgaagca atctgaattg atgcgccaat tgaacaacga accagatgct
     7561 ttctttgctt ttgtacgtgg cgtaacggaa actactgtaa ttaccagcga caaatacaac
     7621 gatttgattg agaagttgtt tgccaacgaa aaactcatta cctcaaccca agatttggca
     7681 tctattactg taaaattgcc gcgtatcaat attgaaattt acggtattta ttattacatc
     7741 ttgaaacaat tggcatggga aggaatcaat atcattgacg taatttctac tgccaacgaa
     7801 tttactgttt tggtttcgca agacgatgtg gacaaagcat ttaaaacttt gatgcagtta
     7861 aatagaggtt tctaaaaatt aacgttccat cctcgttttg ataaaaccgc ataagttgtg
     7921 gttaactcta acgcatgctt tagcttcgca aatgaatgag tgatatttaa ttgatttcca
     7981 cttatttgag gctctaaatt ttgtgggaat tccaatgtgc tcgcgttcgg taaattttct
     8041 aattgataca accatttgta gagcaattga atggattcgt tgtcgctcca ctgcactgat
     8101 tgtgcttcaa tagaacaaac aaattctatc ttttcagagg cacgggtgag taacacattc
     8161 aacctatttc ttcccgattg ggtgttgagt ggaccaaaat gccagctaaa ttgtccttcc
     8221 tcattgggcg catatcccaa actaatgatt aacacttcac attcgtcgcc ttgcaccttt
     8281 tccaagggtt taaaaaacaa ggtgttttca tctatttttt gttgaaccaa tgctaattta
     8341 ttttgcggaa ttgctttaaa aatagcatct atttgttcag cagaaaaagc caccactcca
     8401 atagttttat ttgttggaag atgcttatct aacaattcca caactttgtt tgcttcgtgt
     8461 tcgtttttgc ggttaacaaa acgtccttgt tgacaaaaat aacgttgaat acagttgtta
     8521 tctaccggat aggacggata tgccagcaat tgattgttgt aaaaatgttc gtttgaaaaa
     8581 gcaattaact gcgcatgttt actacgatag tgatgcgtaa gcgtatgatt ggcaaaataa
     8641 taactagcat gatgcaacaa actcaccact tcttgtgcgc ccgcctgaaa ataattggta
     8701 ggactcatct gctgttcatc tccagcaatt atggcatatt gacaacggtg caaggctccc
     8761 aatccatttt gaactggcat ctgacttgcc tcgtcaacaa tgcacatatc aaacaaattg
     8821 gtttgcaacg gaaaagcttt ggcaagactt gttggattgg ttaaccaaac aggtttcaac
     8881 gcctttatcc ataaattagc ttctgattgc aacaattctc gcatggttgg gtgctgccgc
     8941 gatttggcaa actctttcac caataaagct tttcctttct ttaattgttg cttgagcatt
     9001 ttttcttccg cagttaaacg ttgagctggt gtgttgagca acttagaata ttcatccaat
     9061 tgctgtttta cctgctgcaa aatagtttgt gctaccaatt ggtgttcgtt tgcttgctct
     9121 gtcaataatt gctctactcg ttgtttcaat tcttctccag aaaaactaga aaatga
//
LOCUS       DAOQVQ010000042         9118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8475_length_9118_cov_3.743131, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000042 DAOQVQ010000000
VERSION     DAOQVQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_9118_cov_3.743131"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..792)
                     /locus_tag="PKN22_02405"
     CDS             complement(58..792)
                     /locus_tag="PKN22_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNS41584.1"
                     /translation="MKKFNFFGLLLTLVLAVGCGTKKNSATTGDNSMNALDWEGVYIM
                     EQQNDLPEIQLTLKSNNTYQIIVSNWNKQPDNTYFEGKFTWDKTGGFIQMDQLISNIN
                     TSKLKVGENVLWAKDGNIENIKFIKMDVNPALTEKYWKLVAVNDIKITNKDFIETEPH
                     IMFKSEFNHVRGNDGCNQFQGKFEIKGNKISFERMLSTLKACPDVFASTEFRKLLSEE
                     ITFEVKNDKLTLKTKNDTFQFEAVYF"
     gene            909..1619
                     /locus_tag="PKN22_02410"
     CDS             909..1619
                     /locus_tag="PKN22_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41585.1"
                     /translation="MRYFLTFFSCFLFSIAFSQCESVLFKGKVFDTLRPQSFYNMMIV
                     NRNTGKGVFGQPNGHFSVYVNNGDSVTISIKGYDLIHVVIKADENCRFTKDFPIIGKP
                     YEFEEVVVRPVKTLVQIKEEREALVMVETRTVKGLEVIQSPITALYQAFSRTERNKRK
                     VEEWKFQDNKRDIVKDLLRTYVAYDVVKLTEDEFDDFIQFLNMDPDFLRTATEWELIE
                     FIKGKYEHFMYLRYHQEE"
     gene            1638..4535
                     /locus_tag="PKN22_02415"
     CDS             1638..4535
                     /locus_tag="PKN22_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNS41586.1"
                     /translation="MKKHKHFVVIVALLFSVPLLFSFLKNSKNINNETTSWAAQKLAT
                     LSLEQKIAQFFMVAAYPAKGDAHMTEIEQLIVKNEIGGVIWFPENKENFLKYAGKLQK
                     ASSNIPLFYGLDAEWGVSMRMKGEARFPYAYTIGAANDLALSEKIAKMMAYECAELGF
                     QFNFSPVADVNSNPKNPVIGFRAFGDNPAEVGKQVAAFVRGFEASGVYSSIKHFPGHG
                     DTDKDSHLELPTVKKTVEQLTNEDWLPFREGIKVGATSVMVGHLNVPSIDPSGTPSSL
                     SKIVIKDYLKGKLGFKGLVISDALNMKAVSEKYGEVEVAVKAFEAGCDILLYSNNVGA
                     SIQAIKAKVEKGDISLKEINERCLKILQLKEKSFIKPVKGHSFTQGEQDWARNQLFEK
                     STVLLKNEHNNLPFGDLSKKILHIAIGDKPHEFLSVTDEYAPITRKEFSFEAIEKGVD
                     LSTSDFDKVIVTVHASTVRPKDNYGIPTNINAFLKQLTSSTKNTIVFFGNPLILSQQL
                     ELDAFQSIVVAYENNKFVQNRVAQQLFGAIPFVGKLHTNVSELFKKGMGIATQTNGRL
                     KFSQPEEVGIDPNYLTKIDALVENAIQVKSFPGCQIVAAVDGKIFFRKSYGSQMYDNQ
                     LVENDDIYDIASITKIAASTTALMKLQSEGKFSLDKTLKEYLPTITGNYPMGAIKMRE
                     YLAHQAGLQAWIPFYKKTIIDGKLDPKCYATQPQKGYVQLADNIYIKEAYGDSIRRYI
                     LKGELGAKKYLYSDLSYYFVKLIVEQESNKEFSQFLNDEIYAKMGLHHTLFNPYQSVD
                     LKKIAPTEDDKLYRKQVVRGFVHDPGAAMLGGVGGHAGLFSNATELAAIMQLFLNKGY
                     YGGVQLINPQVIAEYTRAQFKGNRRGAGFDKPVLGSGGGTCDESASFESFGHSGFTGT
                     LAWDDPKNGLNYVFLSNRVYPSAENKKLISMGTRTEIQKILNEALKNRKLN"
     gene            4574..6643
                     /locus_tag="PKN22_02420"
     CDS             4574..6643
                     /locus_tag="PKN22_02420"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HNS41587.1"
                     /translation="MKNCVFLGFLFIHWIAWGQDIVPHKMTETNDRKSLQLAWFASSW
                     DIPYEVKKYHKVEIGAQLPSSVVTQLVSFLQKRGKGLNPFNPSDVSLECVFTAPSGKK
                     ETVYGFYYQPYKRDMRSNTWANDTTSYPWRIRFSPDEIGNWQLAVQLKVRGQEVAKST
                     HFSFNTIKSNHKGYLERSYTGNNQDRYLFYHETGETFFGIGHNITHSDYVRLTPQSSE
                     RHLKWLNELASYGGNFFRLELSSQNALPDWNEYDNYSSKMAEMWEYDQLMDSAFSLGL
                     YFILFRHHVELNYGSWPDNWKDNSYRKGFDLPNFSSYFTHATADQWQKYCLRYILARW
                     GYNPSFAFYGYSEVDNYIKPMRDTEAKTFEEALTIFTDWNKKQFSYIKDSLNNRRIMT
                     LNTYAAIDNLEFKTPEKGMFNYLDVIGFHSYKMKKDANWDRTNMLNRLWDLHKKPVFM
                     EEIGVTDNYLQTYCCIDIDFHNNTWSSAFSGSPAIGMHWWWDRGLHDFGFYMQYKELA
                     TFLQNEDFRKLNYVPQRWKDKNAVTKATLEAFYLTSENQERAIGWLHNATAYWRNSFT
                     INSCMQQLIARDSLQFACQMEDGYVIPPLKANYQEEKLKDKYTDVGGIQFYTKGLQNN
                     PTFVVTGLKKKTNYTIEFYSTRTNSKINSNYTQTLKSNGRGQIKPVVPNLDSLQLDYG
                     FKVKIAN"
     gene            6731..7870
                     /locus_tag="PKN22_02425"
     CDS             6731..7870
                     /locus_tag="PKN22_02425"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNS41588.1"
                     /translation="MKFGTKAIHAGVKPDESTGAIMTPIFQTSTYVQDGVGNHKGYEY
                     SRTLNPTRHALEKSLAAIENGKHGACFGSGLAAIDCVIKMLNPGDEVISTNDLYGGTY
                     RLFNTIFAKYGIVFHFVDMTNVAAIEEKVNSNTKLIWVETPTNPMLNIIDIEAVAQIS
                     KKHGVMLGVDNTFASPYLQNPLDLGADIVMHSVTKYFGGHSDVVMGALICNDDKLAAE
                     MYRIQNSSGAVTAPMDSFLVLRGIKTLHLRLQRHCENGEKVARFLASHPKVDKVYWPG
                     FETHPNHAIAKKQMRGFGGMVSFTLKGNVLEDALKLVKNVKIFALAESLGGVESLIGH
                     PATMTHASIPKEEREKTGVVDTLIRLSVGVEDAEDLIADLEQALN"
     gene            7940..8653
                     /locus_tag="PKN22_02430"
     CDS             7940..8653
                     /locus_tag="PKN22_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS41589.1"
                     /translation="MNKIIIITGVSSGIGRAFVEHYLAAGEKVIGLGRKNDIQHDNYT
                     FFPCDFLQPLDFQFLKPLIENSQEILLINNAGVIGNVERISEQNISDIQEVMQVNAIA
                     PMLLCQFVLQHFPIHFPLTIINISSGAGKNPLPSCASYCASKAALDLFSRTIYLEETE
                     RKRAIKVYSVAPGVVDTPMQLKMRSSNEQSFSSVSKFKTYHAQNELYSPAIVVQKMMV
                     LLAKNYTGEIIYSLRDISI"
     gene            8808..>9118
                     /locus_tag="PKN22_02435"
     CDS             8808..>9118
                     /locus_tag="PKN22_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41590.1"
                     /translation="MKKIYIGLTTLLFSSLAMGQSFNPQNARQGESVEYCHQHKLLNE
                     YKTSNPELYNTIMQGQAELNAALKNYQPEKDDQVYTIPVVFHIIHNGGTENISDEQIY
                     D"
BASE COUNT         2991 a         1528 c         1772 g         2827 t
ORIGIN      
        1 catgaaaaaa gctatcccga agaatagctt ttagaatcat tttattgtaa ttattattta
       61 aaaataaacg gcttcaaatt ggaaggtgtc gttttttgtt tttaaagtca acttatcgtt
      121 tttaacttca aatgtaattt cttcactcaa cagtttacgg aattcggtag aagcaaacac
      181 atccggacaa gcttttaagg tagagagcat tctttcaaag gagattttat tacctttaat
      241 ttcaaatttc ccttggaatt gattgcaacc atcgtttcct ctaacatgat taaattctga
      301 tttaaacata atatgcggtt cggtttcaat gaaatcttta ttggtaattt tgatgtcgtt
      361 tacagcaact aatttccagt atttttctgt taacgctggg ttaacatcca tttttataaa
      421 ctttatattt tcaatgttgc catcttttgc ccataaaaca ttttcaccta cttttaattt
      481 tgaagtgttg atatttgaaa ttaattggtc catttgtatg aaaccacccg ttttatccca
      541 tgtaaatttc ccttcaaaat aagtattatc tggttgctta ttccaatttg aaacaattat
      601 ttgataggta ttgttcgatt ttaacgttag ctggatttct ggcaaatcat tttgctgctc
      661 catgatgtaa acaccttccc aatctaatgc gttcattgaa ttatctcctg ttgttgcact
      721 gtttttctta gttccacatc ctactgctaa aacaagtgta agcaataagc caaaaaaatt
      781 aaactttttc attttacatt attttttgac aaagttgtca aaagttgtgc cacaaaaaaa
      841 ataaaataac attttttaaa taaatagtct ggtatctttt ttgttatttt gttgttacat
      901 ccaaaactat gcggtatttt ttaacttttt tcagttgttt tttattctcc attgcgtttt
      961 cacaatgtga atcggttttg tttaaaggaa aagtgtttga caccttgcga ccccaatcgt
     1021 tttacaacat gatgattgtt aaccgcaaca caggaaaagg agtttttggt caacccaatg
     1081 gtcatttttc tgtttatgta aataatggcg attctgttac aatttctatt aaaggctatg
     1141 acttaattca tgtggttatt aaagcagatg aaaattgccg ttttacaaag gattttccta
     1201 tcattggaaa accgtatgaa tttgaagaag ttgtggtgcg tccagtaaaa acattggtgc
     1261 agataaaaga agaacgtgaa gcattggtaa tggtagaaac ccgcaccgta aaaggattgg
     1321 aagttattca gagtccgatt actgctttgt atcaagcatt ttctagaaca gaacgcaaca
     1381 aacggaaggt ggaagaatgg aaattccaag acaataaacg cgatatcgtt aaagatttat
     1441 tgcgtaccta tgtggcatac gatgttgtga aacttaccga agatgaattt gacgatttta
     1501 ttcagttcct aaatatggat cctgatttct tacgtacggc aactgagtgg gaattaattg
     1561 agttcataaa agggaaatac gaacatttta tgtatttacg ataccaccaa gaagaataac
     1621 tccacacctt attttcgatg aaaaaacata aacattttgt agtaatagta gcactgcttt
     1681 ttagtgttcc gctgctattt tcatttctta agaatagcaa aaatataaac aatgaaacaa
     1741 cctcgtgggc tgctcaaaag ttggcaaccc tttcgttgga acaaaaaata gcacaatttt
     1801 tcatggtagc ggcctatcca gcaaaagggg atgcgcacat gactgaaatt gaacaactca
     1861 tagtcaaaaa tgaaattggg ggtgtgattt ggtttccaga aaacaaagaa aatttcttaa
     1921 aatatgctgg aaaattgcaa aaggcttcat ccaacattcc tttattctat ggtttagacg
     1981 cagaatgggg cgtgagtatg cgcatgaaag gcgaagcacg ttttccgtat gcctatacaa
     2041 ttggtgcagc taatgatttg gctttgtctg aaaaaattgc caagatgatg gcttatgaat
     2101 gtgccgaatt gggctttcaa tttaatttta gtcccgttgc cgatgtaaat tccaatccta
     2161 aaaatcctgt tattggtttt agagcttttg gcgataaccc agcagaagtt ggcaagcaag
     2221 tagctgcttt tgttagaggt tttgaagcaa gtggggttta tagttctatc aagcattttc
     2281 cgggacacgg agatacagac aaagattcac atttggagtt gccaacggtg aaaaaaacgg
     2341 tggaacaatt aaccaacgag gattggttgc cgtttagaga aggcatcaaa gttggtgcaa
     2401 cctcagtaat ggttggacat ttaaacgttc cgtccataga cccaagtggc acgcctagta
     2461 gtttgtctaa aattgtaatc aaagattatt tgaaaggaaa attgggcttt aaaggcttgg
     2521 ttatttcgga cgctttgaac atgaaagcag tttcggaaaa atacggtgaa gtggaggttg
     2581 ctgttaaagc atttgaagca gggtgcgata ttttgttgta ttccaacaat gttggtgctt
     2641 ccattcaggc catcaaagca aaagtggaaa aaggcgatat ttcattaaaa gaaatcaatg
     2701 aacgctgttt gaaaattctt caattgaaag aaaaaagttt catcaaacca gttaaaggtc
     2761 actcatttac ccaaggagaa caagattggg caagaaatca actttttgag aaaagtacag
     2821 ttttactaaa aaacgaacac aataatttgc catttggtga tttaagtaaa aaaatccttc
     2881 acattgcaat tggcgacaaa ccgcatgagt ttttaagtgt aacggatgaa tacgcaccca
     2941 tcactcgtaa agaattttct tttgaagcaa ttgaaaaagg ggttgattta tctacttctg
     3001 attttgacaa agtcatagta acggtgcacg cttcaacggt tcgtccaaaa gacaactacg
     3061 gcattcctac taatatcaat gcatttttaa aacaactaac aagttctact aaaaatacaa
     3121 ttgtcttttt tggcaatccc ttgattttaa gtcagcagtt ggaactagat gcatttcaat
     3181 ccattgttgt agcttatgaa aacaacaaat ttgtgcaaaa cagagtggca caacaacttt
     3241 ttggagccat tccatttgtt gggaaattgc acaccaatgt tagcgaactg tttaaaaaag
     3301 gaatgggcat tgcaacccaa accaatggtc gccttaaatt ttcacaaccc gaagaagtgg
     3361 gcatcgaccc aaactatctc actaaaattg atgcgttggt tgaaaatgcc attcaagtaa
     3421 aatcgtttcc aggttgtcaa attgttgctg ccgtggatgg aaaaatattt ttcagaaaaa
     3481 gctatggttc acaaatgtac gacaatcaac tggtagaaaa tgatgatatt tatgacattg
     3541 cttccattac caaaattgct gcatcaacaa cggctttgat gaaattgcaa tcggaaggga
     3601 aattttcatt ggataaaaca ctcaaagaat atttgccaac catcacagga aattacccaa
     3661 tgggagctat aaaaatgcgc gaatacttag cacatcaggc aggattacaa gcgtggattc
     3721 cattttataa gaaaaccatt attgatggca aactagaccc gaaatgttat gctacgcaac
     3781 cgcaaaaagg atatgtgcaa ttggcagata atatttacat caaagaagcg tatggcgatt
     3841 ctattcgcag gtatatttta aaaggtgaat taggagcgaa aaaatatttg tacagtgatt
     3901 tgagctatta ttttgtaaaa ttaattgttg aacaagaatc caacaaagag ttttcgcaat
     3961 ttctaaacga tgagatttat gcaaaaatgg gcttgcatca cacgttgttt aatccgtatc
     4021 aatcggttga tttgaaaaaa attgctccaa ccgaagatga taaactgtac cgcaagcaag
     4081 ttgttcgagg atttgtgcac gacccaggag cagccatgtt gggaggtgtt ggtggtcacg
     4141 ccggattgtt ttccaacgca acagaattag cagccatcat gcaattattt ttaaataaag
     4201 gttattacgg tggtgttcaa ttaattaacc ctcaagttat tgcagaatat acacgtgctc
     4261 aatttaaagg caaccgacga ggagctgggt ttgacaaacc agtgttgggt agcggtggtg
     4321 gaacctgcga tgaatcggct tcgtttgaaa gctttggtca ctctggattt actggaacct
     4381 tggcgtggga tgatcccaaa aatggtttga attacgtgtt tttatccaac agagtatatc
     4441 caagtgccga aaacaaaaag ttgatttcaa tgggaacacg caccgaaatt caaaaaatat
     4501 taaacgaagc attgaagaac agaaaactca attaattagt taactttgtt agactaacaa
     4561 caacgtttgt actatgaaaa attgtgtttt tttaggcttt ttattcattc attggatagc
     4621 ttgggggcaa gacattgtgc ctcacaaaat gacagaaacc aacgatagaa aatcgcttca
     4681 acttgcatgg tttgcctcct cttgggatat tccttacgaa gtaaaaaaat atcacaaagt
     4741 tgaaataggt gcacaacttc catcgtcggt tgtaacgcaa ttggttagtt tcttgcaaaa
     4801 acgaggaaaa ggcttaaatc cgttcaatcc ttccgatgtt tctttagaat gtgtgtttac
     4861 agctccttct ggaaaaaaag aaaccgttta tggattttat taccaaccct acaaaagaga
     4921 catgcgctcc aatacgtggg caaacgacac aacatcttat ccgtggcgta ttcgtttttc
     4981 gcccgatgaa attggcaatt ggcagctggc tgttcaatta aaagtcagag ggcaagaggt
     5041 tgcaaaatcc acccattttt cattcaatac cattaaaagt aatcacaaag gttatcttga
     5101 aagaagttat accggcaata accaagaccg ctacttattt taccacgaaa caggggaaac
     5161 attttttgga attgggcaca acattaccca ttccgactat gtgcgtttga cccctcaaag
     5221 cagtgagcga catttaaaat ggctcaatga attagcatct tatggaggca atttctttcg
     5281 tttagagttg agctcacaaa atgcacttcc tgattggaat gaatacgaca attattcctc
     5341 aaaaatggca gaaatgtggg agtatgacca attgatggat tctgcttttt cgttgggttt
     5401 atatttcatt ctatttcgtc accatgtgga attaaattat ggcagttggc ccgataactg
     5461 gaaagataat tcttacagaa aaggatttga tttgcctaat tttagttcct attttacaca
     5521 tgcaacggca gaccaatggc aaaaatactg tttgcgctat attttggcac gttggggcta
     5581 caatcctagt tttgcatttt acggctattc ggaagtggat aattacatca aacctatgcg
     5641 cgacaccgaa gcaaaaacgt ttgaagaggc gttaactatt tttaccgatt ggaacaaaaa
     5701 acagttttca tacatcaaag attcgttgaa caacagacgc atcatgacgt tgaataccta
     5761 tgcggctatt gataatttgg aatttaaaac acccgaaaaa ggaatgttta actatttgga
     5821 tgtcataggt tttcatagct ataaaatgaa aaaagatgcc aattgggaca gaaccaatat
     5881 gctcaatcgt ttgtgggatt tgcataaaaa acccgtgttt atggaagaaa ttggggtaac
     5941 tgataattat ttacaaacct attgttgcat cgacattgat tttcataaca atacgtggtc
     6001 atcggctttt tctggaagtc cggcaattgg aatgcactgg tggtgggata gaggcttgca
     6061 cgattttgga ttttacatgc aatacaaaga actagcaacc tttcttcaaa acgaagattt
     6121 tcgtaagttg aactacgttc cacaacgttg gaaagataaa aatgctgtta ccaaagcaac
     6181 cttagaagct ttttatctga caagtgaaaa tcaagaacga gcgattggtt ggctgcacaa
     6241 tgcaacagca tattggcgca atagttttac catcaattcg tgtatgcaac aattgattgc
     6301 acgtgatagt ttgcagtttg cgtgtcaaat ggaagatggc tatgtcattc cacctttaaa
     6361 agccaattat caagaagaaa aattaaaaga caaatacacc gatgtgggtg gcattcagtt
     6421 ttataccaaa ggattgcaaa ataaccccac ttttgttgtt acagggttga aaaagaaaac
     6481 caattatacg atagaatttt acagcactcg caccaattct aagatcaata gcaattacac
     6541 gcaaacatta aaatccaatg gacgtggaca aataaaacca gttgttccca acctagattc
     6601 gttacagttg gattatggtt ttaaagtaaa aatagccaat taatttaacg tgtaattcaa
     6661 tttgtaaact gtaaaataag tttatctttg ggcttcaatt tagatttaaa attctgcaaa
     6721 atagaaaaat atgaaattcg gaacaaaagc aattcatgcg ggtgtaaagc cagatgaatc
     6781 aacaggtgcc attatgacgc caatttttca aacatctacc tatgtgcaag atggtgttgg
     6841 aaaccacaaa ggatatgagt attcacgtac gttgaaccct acgcgtcatg ctttagaaaa
     6901 aagtttggca gccattgaaa atggaaaaca cggagcatgt tttggttctg gattagcagc
     6961 tattgattgt gtaatcaaaa tgttgaaccc aggtgatgag gtaatttcta ccaacgattt
     7021 atatggtgga acgtatcgtt tgttcaacac aatttttgca aaatacggca ttgtttttca
     7081 ttttgtggac atgaccaatg ttgctgcaat tgaagaaaaa gtgaacagca ataccaaatt
     7141 aatttgggta gaaacaccaa ccaatcccat gttgaatatt attgacattg aggcggttgc
     7201 acaaatctct aaaaaacacg gcgtgatgtt gggtgttgac aatacatttg cttcacctta
     7261 tttgcaaaat ccgttggatt taggagccga catcgtaatg cactctgtta ccaaatattt
     7321 tggtggtcac tcggatgtgg taatgggagc attgatttgc aacgacgata aattggcagc
     7381 agaaatgtat cgtattcaaa attcatcggg tgctgttacc gctcctatgg attcattttt
     7441 agtattacgt ggaattaaaa ccttgcattt acgtttgcaa cgccactgtg aaaatggtga
     7501 aaaagttgcg cgtttcttgg caagtcatcc taaagtggat aaagtatatt ggccaggatt
     7561 tgaaacacat cctaaccatg caattgctaa aaaacaaatg cgtggatttg gcggtatggt
     7621 gtcgtttaca ctcaaaggaa atgtgttgga agatgcattg aaattggtga aaaacgtcaa
     7681 aatctttgca ttggcagaat cgttgggtgg tgtagaatct ttaattggac atccagcaac
     7741 aatgacccat gcttctattc ctaaagaaga gcgtgaaaaa acaggtgttg ttgacacgtt
     7801 gattcgttta agtgttggtg tggaagacgc agaagattta attgctgatt tagaacaggc
     7861 attgaattaa tccataaata attagtgaga ggttatctac attgtgttgg gtaacctctt
     7921 tttttatatc ttgcaaggta tgaataaaat catcatcata acaggtgttt cttcggggat
     7981 agggcgtgct tttgtggaac attatttagc agcaggtgaa aaagtaattg gcttggggcg
     8041 caaaaatgac attcagcatg ataactatac tttttttccg tgcgattttt tgcaaccgtt
     8101 ggattttcaa ttcttaaagc cactaattga aaacagtcaa gaaattttgt taatcaacaa
     8161 tgctggtgtg attggaaatg tggagcggat cagtgagcaa aatatttcag acattcaaga
     8221 agtgatgcaa gtaaatgcca ttgcaccgat gttgttgtgt cagtttgttt tgcaacattt
     8281 tccaattcat ttcccgctta ctattatcaa tattagttct ggagctggta aaaatccatt
     8341 gccctcgtgc gctagttatt gcgcttccaa agcggcgttg gatttgtttt caagaacaat
     8401 ttatctagaa gaaacggaac gtaaaagagc aattaaagtg tatagtgtgg ctccaggagt
     8461 tgtggataca cccatgcaat taaaaatgcg ctcatccaat gaacaaagct tttcatctgt
     8521 ttctaaattt aaaacctatc atgctcaaaa cgagttgtat tcaccagcaa ttgtagttca
     8581 aaaaatgatg gtgcttttag caaaaaatta tactggtgaa attatttatt cattgcgcga
     8641 tatttccatt taaaatgtgt tgaaattcat ttgtttgggg aattaatgtg tttaaaatgt
     8701 aataaaatcg ccattcattt gtattaatta tttttccaac tcaatgttta ttcgtaaatt
     8761 gtgagcactt tttgattaaa actattaaaa acaaaactat aaacccaatg aaaaaaattt
     8821 acatcggatt aactacgttg ttgttctcat cacttgcgat ggggcaatcg tttaatccac
     8881 aaaatgcacg tcaaggtgaa agtgtggagt actgtcacca acataaattg ttgaatgagt
     8941 acaagacatc caatcctgaa ttgtacaata caatcatgca aggtcaagct gaattaaatg
     9001 cagcgttgaa aaattatcag cctgaaaaag atgatcaggt ctatactatt ccagttgttt
     9061 ttcatattat ccataatgga ggaactgaaa atatttcgga tgaacaaatt tatgatca
//
LOCUS       DAOQVQ010000043         9062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8541_length_9062_cov_4.337293, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000043 DAOQVQ010000000
VERSION     DAOQVQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_9062_cov_4.337293"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKN22_02440"
     CDS             complement(<1..522)
                     /locus_tag="PKN22_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNS41591.1"
                     /translation="MKKAILSFGCFAALSISFGQDCSKIFISEYVEGHGNNKAIEIYN
                     PTNSPVDMSQYFLQRYSNGATTASAQGTADAKTIQLSGTIPAKGTLVYVIDKRDPNGT
                     GQNAPIWDELEAKGDVFLCPDYSANNVMYFNGNDALLLAKGDATNPNSAQTVLIDLFG
                     KIGEDPDVVSGGNY"
     gene            complement(799..1686)
                     /locus_tag="PKN22_02445"
     CDS             complement(799..1686)
                     /locus_tag="PKN22_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HNS41592.1"
                     /translation="MATIDLLITVVLILIMFGIGISLTFRDLQEVFLKPKALLVALSS
                     QMLLLPLIAFGISTIFDLPLYIKIGLILLAASPGGTTAGYITYLFSGNVGLSIVLTTI
                     NSVLTIFTIPLIVNLSLHLFYGAGNTFHLPFFETMREIFMVTLIPVGMGIAFRKWKVN
                     TAQKIAKITKPISIVLLGCVFLLKFIGTNQEAGVTMHEIGEILPYALLLNVLCLLTGF
                     GIASVFKLGQKNQITISTESAVHNTTIAFLVAGSIMHQPDFGKVSLVYALFSFWTALA
                     FCLFIIKWKKPSVKNVNKL"
     gene            complement(1704..4121)
                     /locus_tag="PKN22_02450"
     CDS             complement(1704..4121)
                     /locus_tag="PKN22_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS41593.1"
                     /translation="MKQLFLLAFIVISTNLLAQSAKVVGKVTDQDNKPIFGATVIYKQ
                     DVTLGAYTDEEGNYLIEVPVGKAILIYRYSGMVTDTVSVDVVAGEAKVVDFQLFPIVT
                     QIQGVEIKVGKFDRPIEEQTVSLEIIKPELIESKNTRSIETALDQTPGLNILDGEPQI
                     RGGSGFTFGVGSKVAVIVDDMPMLSGDAGRPEWGFIPVENIHQIEVIKGASSVLSGSS
                     ALSGAIHIRTAYPKLKPLTKVNVYSGFYSKPNIEGGTWWKLPPLIAGVNFLHSRMAGN
                     WDIVIGGNFNYDHGYMGPPVQDSLVDVFSPDTISNFSERKMTSQRARINFNIRHRSKR
                     LRGLSYGVNGNFMYAESPLVFAWLNDSSGLYQGYPGAVFLQNQFLFNVDPFLSYISQT
                     GGKHFLRTRVLYADNVMTANQSNRSTTYYGDYMFQKTIPWLKGIDIIAGLTGKYTDSY
                     AQMYVGGGTPNNDFLNISAYTQVEKKFFKLLNLSIGGRLEYFQVNDSITALKPIFRAG
                     ASVRVFQETYFRASYGQGYRFPTITERFIRTGVGNFGVFANPHLKPESSWNAEIGVKQ
                     GLKLGSIYGYVDVAGYWQEYKNTIEYLFGYWGSSFVTLSDAFGFKFLNTGRSRVVGID
                     ASFTGKAKLGKKADMVFMIGYNYILPKTLSPNYVYGVDSVITNKVYSYDSTSLDPSKR
                     ILKYRFLHNVKIDMEWTLFNKLAFGGSVKYFSKMENMDRVIKELEDATSGPFMQSLRY
                     MDYFKANQKGVWIFDARISYAITPQHKIAVICANLLNKSYSLRPLKIESPRTLMLQYT
                     FKLDGNS"
     gene            complement(4190..6361)
                     /locus_tag="PKN22_02455"
     CDS             complement(4190..6361)
                     /locus_tag="PKN22_02455"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS41594.1"
                     /translation="MKKIVLSILSIATGLTLNAQTQIGNAGFENWENVSGGSEPVNWN
                     SFLTASGGLNGNAANQLQNSTDVRPGSTGTHSVRLNSRSVLGVKANGVLTLGRVNMGS
                     INASDLANHNYTVTGDANFSEAMTAFPDSIVFWVKFVPNGHNQNARMKATLHDNYNYQ
                     DPETTTSSQHVVATAVLDYPKTNNTWVRKAVPFNYSGPASSIQYLLVTFSTNATPGGG
                     AADDYVWVDDVELIYNEPSFNVSNTTVCAGSSVNFTNTSVINSGVTSYSWSFPGGSPA
                     TSTSATPSVTYSTPGAYNATLTITNQYGSRTKTYNNYITVTALDNATFNYPNASYCSN
                     SANPVPTAVIGGSYSSTTGLVFANSTTGEIDLAASTPGTYTVTHTTNGACPNTSTKQI
                     TIVAAENSTFNYPSNTICMTGGNQTPTVATNGGTFSSSPAGLTFVSTSTGEINVAGSS
                     EGTYAIVYAVAGTCPSTTTVNVTLTSTPSADFNYAQASYCVNAVDPAPSYATGANAGV
                     FSSTPAGLVINPNNGIIDLSLSDAGTYTIENNIAASGSCPSATHTSTITIDDLPTVGL
                     VLTPTTVCTYTTAYMLTGGTPTGGSYSGTGVGAGIFNPAAYPNGGTATVTYSYTDAAT
                     TCTNTATATITIDACAGVEEVATFDAVTVYPNPTSGLIKVDNLVATTTYVVIATTGQV
                     VSTGELNSTEATIDLSNVESGIYLLQLKQENDAKTIRIIKQ"
     gene            6554..7999
                     /locus_tag="PKN22_02460"
     CDS             6554..7999
                     /locus_tag="PKN22_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS41595.1"
                     /translation="MGNSWKAKKSIASLQQEASSGNALKRSLSSASLVALGIGAIIGA
                     GLFSLTGIAAAEHAGPAVTLSFVLAAVGCGFAGLCYAEFASMIPVAGSAYTYSYATMG
                     EFIAWFIGWDLVLEYALGAATVGVSWSKYLNQLLESYGMGLPENLINGPFEGGIINLP
                     AIFIVSLLSLVLIRGTKESAVVNNILVILKVAVVLLFIILGWEYIDPSNHQPFIPAND
                     PSKPDFGGMPGIAAGAAMVFFAFIGFDAVSTAAQEAKNPQKGMPIGIIGSLVVCTILY
                     VLFSYVMTGLVPYQTFVGDASPAATAFAATPYTFLKQGLIIAILAGYTSVIMVLLLGQ
                     SRVFYSMSKDGLLPSFFSDVHPKFATPWKTNLIFMVFVSLFAGFVPVKDLGHMVSIGT
                     LLAFCLVCVGILVMRKKMPDAPRAFKTPLVPFVPIAGVITCGYLMYSLPLESWLRLLV
                     WMLIGCAVYFLYGYKHSKLRAENENNSSTND"
     gene            8092..8763
                     /locus_tag="PKN22_02465"
     CDS             8092..8763
                     /locus_tag="PKN22_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS41596.1"
                     /translation="MKVVIADGNHLMRIGLRNTLNTNASIKIVGEVGSSKELLDFIKK
                     TEVDIVLIDYTSEFFDIDVIPKIVSKNPSTHIVAITPEQSAQTLIDALRSGVQSYIKK
                     NCDLDEILEAVVETAKGKKFFCRQILETIRHSAINVEDIDFDWCTCESTSLSEREVQI
                     IKRIADGMTNAEIAEELCLSNHTVNTHRKNILSKLGVKNTAGIVMYAVKMNIVSPNKF
                     LFASE"
BASE COUNT         2856 a         1587 c         1600 g         3019 t
ORIGIN      
        1 atagttacca ccagaaacaa catctggatc ttcaccaatt ttaccaaaca aatcaataag
       61 aacagtttga gcagagttag gattggttgc atccccttta gctaaaagca aagcatcgtt
      121 accattgaaa tacattacgt tgttagcgct ataatcagga cataaaaata cgtcaccttt
      181 tgcttctaat tcatcccaaa ttggtgcatt ttgacctgta ccattaggat ctcttttatc
      241 aataacatat accaaagttc cttttgcagg aatggttcca ctcaattgaa tggtttttgc
      301 atctgcagtt ccttgagcgc ttgcggttgt agctccattg gaataacgtt gcaaaaagta
      361 ttggctcatg tccactgggc tatttgttgg attataaatc tcaatagctt tgttgtttcc
      421 gtgtccttct acgtactcag aaataaaaat cttagagcaa tcttgcccaa aagaaattga
      481 taatgctgca aaacagccga aagaaagtat tgcttttttc atataaagga atttaataaa
      541 attgcgaata gataactacg atgaaagtaa taataggatg tagcatttgt tttgttaaaa
      601 cacaataaac taaggcttaa tttggatcta acagcctttg tattttgtag tgtaaacatt
      661 actttttcat catacaaata attttttctg aaatggtttc aacattattg gaatgtatca
      721 aggacaacac aacagaaatt tcaatgcaaa attaagcaat attattcaac cagcaaaaaa
      781 agaattatta acagatggtt acaatttatt tacattctta acagatggct ttttccattt
      841 aatgataaat aagcagaatg caagagctgt ccagaatgaa aaaagagcat aaaccaatga
      901 tacttttcca aaatcgggtt ggtgcataat ggaaccagca actaaaaaag caatagttgt
      961 attatgtact gcagattcgg ttgaaatggt aatttgattt ttttgtccta gcttaaaaac
     1021 ggatgcaata ccaaaacctg ttaacaagca caaaacattt aaaagcaaag catagggtaa
     1081 aatttctcca atttcatgca tagtaacacc cgcttcttgg ttggtaccta taaattttag
     1141 taaaaacaca catcctaata aaacaattga tatgggtttg gtgattttag caatcttttg
     1201 agctgtattt actttccatt ttctaaatgc gatgcccatt ccaactggaa tcaacgtgac
     1261 cataaaaatc tcgcgcatgg tctcaaaaaa tggtagatga aacgtattgc cagcaccata
     1321 aaacaaatgc aatgataagt tgacaattaa cgggatggtg aaaattgtta acacagaatt
     1381 gatggtggtt aaaacgatgg ataaccctac gttaccacta aataaatagg tgatgtaacc
     1441 agcagttgtt ccacctggac ttgcagccaa taatattaaa cctattttta tataaagtgg
     1501 aaggtcaaaa atggtgctaa ttccgaatgc aattagtggt aaaaggagca tttgtgagct
     1561 tagagccaca agaagtgctt ttggttttaa aaacacttct tgtaagtctc taaaagtaag
     1621 tgatatacct attccaaaca tgattagaat aagtactacc gttattaata aatcaattgt
     1681 tgccattgat tttagtgcat caattagcta ttgccatcga gtttaaatgt gtattgcaac
     1741 atcaatgtgc gaggcgattc tattttcaac ggtcgtaaag agtatgattt attcaataaa
     1801 ttggcgcaaa tcactgcaat tttatgctgc ggagtaatgg cataggaaat acgtgcatca
     1861 aaaatccata cgcctttttg gtttgctttg aaataatcca tataacgtaa actttgcata
     1921 aaaggaccag atgttgcatc ttccaattct tttattactc tgtccatgtt ttccatttta
     1981 ctgaaatatt taacacttcc tccaaaagct aatttattaa acaaggtcca ttccatatca
     2041 attttaacat tgtgtaagaa acggtatttc aaaatgcgtt tagatgggtc taaagatgtt
     2101 gaatcgtagc tatacacttt gttggtaatt acagagtcaa caccataaac ataatttggg
     2161 cttaacgttt ttggtaaaat gtaattgtat ccaatcataa acaccatgtc tgctttttta
     2221 cccaattttg ctttacctgt aaaagaagca tcaataccca caactctaga tcgacctgtg
     2281 tttaagaact taaatccaaa agcatcgctt agcgtaacga aggaagaacc ccaatatcca
     2341 aacaaatatt ctatggtgtt tttatattct tgccaataac cagcaacatc cacataacca
     2401 taaatgctac caagttttaa tccttgtttt acaccaattt cagcattcca gctactttct
     2461 ggttttagat gagggtttgc aaatactcca aagtttccaa ctcctgtgcg gataaagcgc
     2521 tctgtgatag ttgggaaacg gtatccttgt ccataagatg ctctaaaata ggtttcttga
     2581 aacacacgta cgcttgcacc agctctaaaa attggtttta aagctgtgat agaatcattg
     2641 acttgaaaat attccaatct accaccaatt gaaaggttta ataacttaaa gaatttcttt
     2701 tcaacttgtg tataggcaga aatatttaag aaatcattat ttggcgttcc acctcccaca
     2761 tacatctgtg cataagaatc ggtgtattta cctgttaatc cagcaataat atcaattcct
     2821 tttagccatg gaatggtttt ttgaaacatg tagtcaccat aatacgtcgt agatctgttc
     2881 gattggttgg cagtcattac gttatccgca tataaaacac gtgtacgtaa gaaatgttta
     2941 cctcctgttt gagatatata gcttaaaaat gggtcaacat taaacaaaaa ttggttttgt
     3001 aaaaatacag ctcctggata tccttggtat aaacctgatg aatcattcaa ccaagcaaaa
     3061 acgagtggag attcagcata cataaagttt ccattaacac catagctcaa ccctcttaaa
     3121 cgtttacttc tgtgacgaat gttaaagttg atacgtgcac gttgtgaagt catttttctt
     3181 tccgaaaaat ttgagatagt atcaggtgaa aaaacatcta ctaaagaatc ttgtacagga
     3241 ggtcccatgt atccatgatc gtaattaaag ttaccaccaa ttacaatgtc ccagttacct
     3301 gccatgcgtg aatgtaagaa gtttacacca gcaattaatg ggggcaattt ccaccatgta
     3361 ccaccttcaa tgttgggttt agaataaaat ccactgtaaa catttacctt cgttaatggt
     3421 tttaatttag gatatgccgt gcgaatatgg atggcacctg acaacgcact acttcctgaa
     3481 agcaccgaag atgcaccttt aattacctca atttgatgga tgttttcaac tggaataaag
     3541 ccccattcag gtctacctgc atctccagac aacattggca tatcgtcaac aattactgca
     3601 actttagaac ccacaccaaa agtaaaacca ctacctccac gaatttgagg ctcaccatcc
     3661 aaaatattca atcctggtgt ttggtccaaa gctgtttcaa tgcttcgggt gtttttgctt
     3721 tctattaatt ctggcttgat gatttctaaa gaaacagttt gctcttcaat tgggcgatca
     3781 aatttaccaa ctttaatttc tactccctga atttgagtga ctattgggaa aagttgaaaa
     3841 tcaaccactt ttgcttcgcc ggcaacaaca tcaacactca cagtatctgt aaccattcca
     3901 gaataacggt aaattaaaat tgcttttcca acaggaactt caatcaaata attaccttct
     3961 tcgtcggtat aagcaccaag tgttacatct tgtttataga ttaccgttgc gccgaagata
     4021 ggcttgttat cttgatcggt tactttacct accacttttg cactttgtgc caataagtta
     4081 gtagaaataa caataaacgc taataagaat aactgtttca tagaatgatg tattaaaaaa
     4141 gtccctcact tcaaaaggaa gataagggac ttcaaaaata taaaaatttt tactgtttga
     4201 taattctaat tgtttttgcg tcgttttctt gtttcaattg caacaagtaa attccacttt
     4261 caacattcga taaatcaatt gtagcttcag tagagtttaa ttctccagtg cttacaactt
     4321 gtcctgttgt tgcaataaca acatacgttg tagttgctac taaattatca actttaatca
     4381 atccactagt tggatttgga taaactgtta ctgcatcaaa agttgcaact tcttcaacac
     4441 ccgcacaagc atctatagtg atggttgctg ttgctgtgtt agtacaagtt gttgcagcat
     4501 ctgtgtatga ataagtaacg gttgcagttc ctccatttgg ataagctgca gggttaaaaa
     4561 taccagctcc aacacctgtt ccagaataag aaccacctgt tggagttccg ccagttaaca
     4621 tataagctgt tgtgtaagta caaaccgttg ttggagttaa tactaaacca actgttggta
     4681 aatcatcgat tgtaattgta gatgtatgtg ttgcagatgg acaagaacca gaagctgcaa
     4741 tgttgttttc tatagtataa gtaccagcgt cactaagtga taaatctata ataccattgt
     4801 ttggattaat taccaaacct gctggagttg atgaaaacac accagcatta gctcctgttg
     4861 cataacttgg cgcagggtct actgcattca cacagtaact tgcttgtgcg tagttgaaat
     4921 ctgcacttgg agttgatgtt aaagttacat tcacagttgt tgttgaagga catgttccag
     4981 caactgcata aacaattgca taagtacctt cagaactacc agccacgttg atttcacctg
     5041 ttgaagtgct tacaaaagtt aaaccagctg gtgaagaaga aaatgttcca ccatttgttg
     5101 ctacagttgg agtttggtta ccacctgtca tacaaattgt attagaagga tagttgaacg
     5161 ttgaattttc tgcagcaaca attgtaattt gttttgtact agtgtttgga cacgcaccat
     5221 ttgttgtgtg tgttacagtg taagttccag gcgtactagc tgctaaatca atttctccag
     5281 ttgttgaatt agcaaaaacc aaacctgttg ttgagctata agaacctcca attacagcag
     5341 taggaacagg atttgcacta ttggaacaat aactagcatt tggataatta aatgtagcat
     5401 tgtctaaagc ggttacagta atgtagttat tgtatgtttt tgttctacta ccatattgat
     5461 tggtaatcgt taaagttgca ttgtatgcac ctggtgtgct gtaagttacc gatggagttg
     5521 cagaagtact agttgcagga gaaccacctg ggaaagacca agaataagaa gtaactcctg
     5581 aattaattac agatgtattg gtgaaattaa cagatgaacc tgcacaaaca gttgtgtttg
     5641 aaacattaaa tgaaggttcg ttgtaaatta attctacatc atccacccaa acataatcgt
     5701 ctgcagctcc tccacctggt gttgcatttg ttgaaaatgt taccaacaag tattgaatgg
     5761 aagaagcagg tccactgtaa ttaaatggga cagcttttct aacccaagta ttattagttt
     5821 tagggtaatc caaaacagcc gttgcaacca catgttgtga agatgtagtt gtttcagggt
     5881 cttgataatt gtaattatcg tgtaatgttg ctttcatacg tgcattttgg ttgtgaccat
     5941 ttggaacgaa ttttacccaa aatacaatag aatcaggaaa agcagtcatt gcctcagaaa
     6001 aatttgcatc accagttacg gtatagttat ggtttgctaa gtctgaagca ttgatactcc
     6061 ccatattaac tcttcctaag gttaaaactc cgtttgcttt aacccctaaa acagaacgtg
     6121 aattcaaacg tacactgtgt gttcctgttg agccaggtct aacatctgta gaattttgca
     6181 attgattagc agcgtttcca tttaaacctc cacttgcagt taagaaactg ttccaattta
     6241 caggttctga cccaccagaa acattttccc aattttcaaa tcctgcatta ccaatttgag
     6301 tttgagcatt taatgtaagc ccagtagcaa ttgataaaat tgataaaact atttttttca
     6361 taaaataaaa tttatttgtg ttggcaaatg tatgtaactt ttactcaaat caacaaaatt
     6421 ttaacatttt tacttgttaa cacattgatt gtcaataaaa ataatataaa aatagcaaaa
     6481 caaaattcga tatttctttg attttcttct tttttagtta gttttgacaa tctctaaatt
     6541 aacttttttg tatatgggaa attcgtggaa agccaaaaaa tcaatagcta gcttacagca
     6601 agaggcatca agtggtaatg cattaaaacg ttcgttaagc tctgcttcat tagtagcgct
     6661 tggtattgga gccattattg gtgcaggttt gttttcattg acaggtattg cagctgcaga
     6721 acatgcggga ccagcagtta cgttgtcgtt tgtattagca gcagttggtt gtggttttgc
     6781 aggtttgtgt tatgctgaat ttgcatcaat gattcctgtt gccggaagtg cctatactta
     6841 ttcttatgca actatgggtg agtttattgc ttggtttatt ggatgggatt tagttttaga
     6901 atatgctctt ggcgctgcca cggttggtgt gagctggtct aaatacctca atcaattgtt
     6961 ggaatcgtat ggaatgggat tacccgaaaa tttaattaat ggaccttttg aaggaggaat
     7021 tattaatctt ccagctattt ttattgtttc attgctttcg ctagtattga ttcgtggtac
     7081 aaaagaatct gcagtagtca ataatatttt ggtgatttta aaagttgctg tggtattgtt
     7141 gttcattatt ttaggatggg aatacattga tccgtctaat catcaacctt ttattcctgc
     7201 aaatgatcca tcaaaaccag attttggagg aatgcctgga attgctgctg gtgctgctat
     7261 ggtgtttttt gcttttatcg gttttgatgc ggtatccacg gctgctcaag aagctaaaaa
     7321 tccacaaaaa ggaatgccta ttggtattat cggttcatta gttgtttgta ctattttata
     7381 tgttttgttt tcttacgtaa tgacaggttt ggtaccctat caaacatttg taggtgacgc
     7441 atctccagct gctacggctt ttgcagcaac accttataca tttttaaaac aaggtttaat
     7501 tattgccata ttggctggtt atacttcggt tatcatggtg ttgttgttag ggcaaagccg
     7561 cgtgttttat tctatgagta aagacggatt gttgccttct ttctttagcg atgtacatcc
     7621 aaagtttgca actccttgga aaacaaattt aatcttcatg gtttttgtat ccctatttgc
     7681 aggttttgtg cccgtaaaag atttaggaca catggtaagt attggtactt tattggcttt
     7741 ttgtttagtg tgcgttggta ttttagtaat gcgtaaaaaa atgcccgatg ctccacgtgc
     7801 tttcaaaaca ccattggttc catttgtgcc aattgcaggt gttattactt gtggttattt
     7861 gatgtattct cttcctctag aaagttggtt gcgtttattg gtttggatgc taattggttg
     7921 tgctgtttat ttcctatatg gctataaaca cagtaaatta agagcagaaa acgaaaataa
     7981 ttcttcaacc aacgattaaa caatttgctg taattgtttt aaaattaaat ttagaatctt
     8041 tctaaattaa ccgatttttt ataattttgc gattcgatat aattcttaat tatgaaagtc
     8101 gtaatagctg atggcaatca tttaatgcgt ataggtctta gaaacacatt gaacaccaat
     8161 gcgtcaatta aaattgttgg agaagttggc tcgtcaaaag aattattgga ttttattaaa
     8221 aaaacagaag ttgacattgt tttgatagac tacacctctg agttttttga tattgatgtt
     8281 attcctaaaa tagtaagcaa aaatccatct acgcatattg ttgcaattac tcccgaacaa
     8341 agtgcacaaa cactcattga tgcattacgt tctggtgttc aaagctacat caagaaaaat
     8401 tgcgacctcg atgaaatact tgaagcagtt gtggaaaccg caaagggtaa aaagtttttc
     8461 tgccgtcaaa ttttagaaac cattcgtcac tcagcaatca atgtagaaga tattgatttt
     8521 gattggtgta cgtgtgagtc aacctcttta tccgagcgag aagtgcaaat cattaaacgc
     8581 atagcagatg gtatgaccaa tgcagaaatt gccgaagaat tgtgcttgag taatcacact
     8641 gtaaacacac atcgcaaaaa catactttcc aaattaggag ttaaaaatac agcgggtatt
     8701 gtcatgtatg ccgtaaaaat gaacattgtt tcgcctaata aattcttatt tgcttctgaa
     8761 taatttttca gaaactatat cttcttttga tagaaactta ccttctaatt gcattcataa
     8821 attctacttt aatttctttt tttaccattt ttttgcaact ttatttataa ttcattggtg
     8881 aatatttcta ttttcgcaag ttattaattg cagttttctt ttgattacgc attcaaaact
     8941 taaatatgaa ctacaaaaaa attaatgtct taacgggttg gttggttttt ctaatcgcaa
     9001 cgtttgttta tctatcaacc attgaatcaa cagtaagttt gtgggactgt ggtgagtaca
     9061 tc
//
LOCUS       DAOQVQ010000044         8986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8626_length_8986_cov_4.186205, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000044 DAOQVQ010000000
VERSION     DAOQVQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8626_length_8986_cov_4.186205"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..943
                     /locus_tag="PKN22_02470"
     CDS             209..943
                     /locus_tag="PKN22_02470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS41597.1"
                     /translation="MEDVQWFESWFDTAYYHILYKNRDDQEAKRFVENLISFLQLPQN
                     SSVLDLACGKGRHSITLAAHGFNVLGVDLSEQSITAAKQFEKENLHFATHDMREVIAN
                     KKFDAVFNLFTSFGYFDTEDDNLKMLQSIHQMLHEKGILVIDFMNATKELAHLVPFET
                     KEIDTIAFQITKKADNQHIYKYIEFEDKGKNHRYMERVQALQLEDFKKLFDLAGFDLL
                     HTFGNFDLEKFDAPTSNRLILIVQKR"
     gene            943..1698
                     /locus_tag="PKN22_02475"
     CDS             943..1698
                     /locus_tag="PKN22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNS41598.1"
                     /translation="MELSLTFIALFAAAISGAVLVTILEHANKQTIIKMLLAFSGGFL
                     LSMAFIHFLPDLYKEEAVSNIGLYILLGFLVQLFLEYFSGGIEHGHVHSHHHGKIPWA
                     IFIALGIHSFIEGIPLAAPFIHDELAHAHLHAHEHHHHVGNSLLMGILFHQVPVSIAL
                     MTLLVSSHIKKSIVWLLMIGFALTTPLGLVAGLFSQSLVEALNFDIILAIVVGMFLHI
                     STTIIFETSENHKVNTLKLVSIIAGFAIAILAF"
     gene            complement(1695..2282)
                     /locus_tag="PKN22_02480"
     CDS             complement(1695..2282)
                     /locus_tag="PKN22_02480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNS41599.1"
                     /translation="MKRIFDIFCSLIILSLFLPFGLILSLLIVLESKGGVFYRQERIG
                     RFGQPFLLFKFRSMRKDADKQGKLTVGMRDARITRTGYFIRKYKLDEFPQFINVIKGD
                     MSIVGPRPEVKEYVDLYTPEQREILNVRPGITDIASLHYFEENELLGKSDNPHKTYVE
                     EIMPAKIALNKQYLANPTLLNDLKIMWKTFVRILS"
     gene            2336..2944
                     /gene="yihA"
                     /locus_tag="PKN22_02485"
     CDS             2336..2944
                     /gene="yihA"
                     /locus_tag="PKN22_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNS41600.1"
                     /translation="MKIKSAVFAVSNTDYKKCPTGDLPEYAFIGRSNVGKSSLINMLT
                     SQKNLAKTSGKPGKTQLINHFLINENWYIVDLPGYGYAKVAKSQREKFEKFIAEYLLN
                     RQQLVNVFVLVDSRLDPQQIDLEFMNWCGEKEIPFSIVFTKIDKLSSSALQKNMAKYK
                     KELQKYWAEIPPVFTSSSETGFGSEKILNYIEQINQAIAGNQ"
     gene            2981..4366
                     /locus_tag="PKN22_02490"
     CDS             2981..4366
                     /locus_tag="PKN22_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS41601.1"
                     /translation="MIIEQLYTKCLAQGAYFISHKGEAAVIDPIREVDQYIELAKANN
                     ATIKYIFETHFHADFVSGHVDLARKTGATIVYGPTAKTNFDCLVATDNQEFKIGDLTI
                     RLLHTPGHTLESSCYLLLDENGKEIALFSGDTLFLGDVGRPDLAIKSDITMEDLAGMS
                     FDSLRNRIMPLPDDVMVYPGHGAGSACGKNMSKETQDLLGNQKRTNYALRADMTRDEF
                     IKEVTEGILPPPQYFPKNALLNKTGYESVDEVIARGSQPLSLAEFKQKIAEGALILDV
                     RNQNDFVQGFIPNSLFIGIDGQFAVWVGTLIQDIKQPIVVVCPEGKETEVATRLARVG
                     YDNALGYLKGGVAAWDGELNTIQNVEATALESVDAQSIVDVRKPGEYNAEHVENCVFY
                     PLDFMSDDLNKLDKEKPLYVHCAGGYRSVIAISLLMQKSNYRNLINVVGGYAAIAKTA
                     VPKTAFVCSSK"
     gene            4373..5266
                     /locus_tag="PKN22_02495"
     CDS             4373..5266
                     /locus_tag="PKN22_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNS41602.1"
                     /translation="MKNNEIAVKTAYFSIFSNFCLALIKGVAGFFGNSYALIADAIES
                     TTDVFSSILVLLGIRYSSRPADENHPYGHGKIEPLLTFVIVGFLITSAVVIAYESCKN
                     IFTPHALPKSWTLYVLGAIIVWKEASYHIVLHKSKQINSTVLKADAWHHRSDAITSIA
                     AFIGISIALFFGKGYEAADDWAALLASVFILYNSYRIFRPALSEIMDENTYEDIIKQV
                     RAVATTVDGVIDTEKCYVRKSGVYYYIDLHATVDGNATVKKGHDISHRLKDALQSNLP
                     FVKDVLIHIEPHDIGEEVNLD"
     gene            complement(5263..6249)
                     /locus_tag="PKN22_02500"
     CDS             complement(5263..6249)
                     /locus_tag="PKN22_02500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNS41603.1"
                     /translation="MKIAVVGATGMVGQVMLQVLREENFPVSELIPVASEKSVGKTVE
                     YAGKEYAVVDLQTAVDMKPAIALFSAGGETSLEWAPKFAAVGTTVIDNSSAWRMDATK
                     KLIVPEINGDQLTKDDKIIANPNCSTVQLVMALAPLHRVYTVKRVIVSTYQSITGTGV
                     KAVEQMENERNGVAASMVYPYPIDKNCLPHCDSFLDNGYTKEEMKLTNETKKILDPAI
                     NVTATAVRVPVVGGHSEAVNVEFSRDFDLGDVRTILHKTAGVVVKDSPITNIYPMPRY
                     AEGKNDVFVGRIRRDESQPNTLNMWIVADNLRKGAATNAVQIAKLLVKNGLV"
     gene            6359..8950
                     /locus_tag="PKN22_02505"
     CDS             6359..8950
                     /locus_tag="PKN22_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNS41604.1"
                     /translation="MQTVMFLLFQVPTVALSQITDDFNDGDFSNNPTWLGTVADYQVN
                     TSQQLQLNAAVAGTSYLYLPHNLTDFNGKEWRFWVRQNFAGSANNYGRIYLIASATDL
                     STDPDGVYLQMGEAGSTDAVRLMQRNGGVTTTICASADGSIANAFAIGVKIIRDNTGN
                     WSLNIDFAGGVNYSAIATGVETTVPTGAYFGYLNVYTSGNITKFYLDDVYAGDQVFDT
                     TPPVMTSVQVISNNQLDVLFSEAVTATSAQTINNYDLIPINSVSSATLDGTNPALVHL
                     QLTTPLVNGNTYTLSSNAIEDLSGNIASNQSLNFTYLVAETPLAGDVLINEFMADPSP
                     VVGLPEVEFVEIYNKSNKVFNVSGWKLGDNATFGTITSGWLLPNQYLVLCPNANVAEF
                     TNAVGVTSFPSLNNAADDIVIQDNTGVQLDKISYTDAWYQNTAKKDGGWTIERINPNE
                     PCSQASNWKASTAVAGGTPGAQNSVHDVTPDSQAAQIAGINILSTNQIEVVFNKMVDS
                     TSLKNAAITVNPTLTENNRTIASIHVASFSMDFVENFAEGQFYTLSIPSVVDCWGNTG
                     DVSATFVLPSQPDSGDVVINEILVNPITGGSDFVELYNKSDKLLDIKNWKIARIYNGV
                     VTDYKTITNSRLLHPKDYLVVTADSNFVMSNYPATIPNKFYALTLPTLSNDTGSVVLT
                     YDKFVFMDTIAVVLDQLVYSSKWHFKLIDDNKGKTLERLDPNRPTQDASNWHTAAESI
                     GFGTPGGKNSQYYPAIHDGEVSLSSETISPDNDGFEDVLLINYTMTSPGMVASFYVYD
                     GAGRKVRTIAQNELLGMEGTIVWDGVRDDGQKALIGTYVLVVNAFATEGGKKFVAKKP
                     FVVAGKL"
BASE COUNT         2786 a         1686 c         1764 g         2750 t
ORIGIN      
        1 cccatacatt aaaagtgtat tgctctacca gctgagctac ggaatctaaa aacagatctt
       61 tttctgttcg tgggtgcaaa aatagtgctt ttattttgtt tcgcaaattt tcaattaata
      121 ttttcaagtt ttattgattt ttattccacc atttgcgata aaaagttggt ttttatttaa
      181 gaatcggaga aatgcctatc tttgcaccat ggaagatgtt caatggtttg aatcgtggtt
      241 tgatacagcg tattatcaca ttttatacaa aaatcgcgac gatcaagaag caaaacgttt
      301 tgtagaaaac ctcatttctt ttttacaatt acctcaaaat agttcggtgt tggatttggc
      361 ttgtggcaaa ggccgtcact ccattacgct tgctgctcat ggtttcaatg tattaggggt
      421 agatctttca gaacaaagca taactgctgc aaaacagttt gaaaaagaaa accttcattt
      481 tgctacacac gatatgcgcg aagtgattgc gaataaaaaa ttcgacgcag tgtttaatct
      541 tttcaccagt tttggctatt tcgacacgga agatgacaac ctaaaaatgc ttcaatccat
      601 tcatcaaatg ctccacgaaa aaggaatttt ggtgattgac tttatgaatg caacgaagga
      661 attggcgcat ctagtaccct ttgaaacaaa agaaattgac accatcgcat ttcaaattac
      721 taaaaaagca gataatcaac acatctacaa atacatagaa tttgaagata agggcaaaaa
      781 ccatcgttac atggaacgtg tgcaagcgtt gcaacttgaa gatttcaaaa aattatttga
      841 cctagcaggt tttgatttgt tacacacttt tggtaatttt gatttagaaa aatttgatgc
      901 acctacatca aaccgtttga ttttaattgt acaaaaacgc taatggaatt aagcttaaca
      961 tttattgctc tttttgctgc tgcaatttct ggtgctgtat tggtcactat tttagaacac
     1021 gccaataagc aaaccattat aaaaatgttg ttggccttta gcggtggctt tttgttgtcc
     1081 atggcattta tccatttttt acccgattta tacaaagaag aagcggtgtc aaacattgga
     1141 ttgtacattc tacttggatt tttagtgcaa ttgtttttag aatatttttc aggtggaatt
     1201 gaacacgggc acgtacacag tcaccaccat ggtaaaattc cttgggcaat ttttatcgca
     1261 ttgggaattc actcttttat tgaagggatt ccgttggccg caccttttat tcacgacgaa
     1321 cttgcacatg cacacctaca tgcacacgaa caccatcatc atgttggaaa ttcgttactg
     1381 atgggaattt tgtttcacca agttcctgtt tctattgcct tgatgacttt gttggtgtcc
     1441 tcacacatta aaaaatcaat tgtttggttg ttgatgatag gttttgcact aacaacgcca
     1501 cttgggttag tagctgggtt gttctctcaa tcattggtag aagcattgaa ctttgatatt
     1561 attcttgcca ttgtagtggg aatgtttttg cacatttcta ccaccattat ttttgaaaca
     1621 agtgagaatc acaaagtgaa caccctcaaa ttggtgagca tcatcgcagg ttttgcgatc
     1681 gcaattttgg ctttttagga aagaatacgc acaaaggttt tccacataat ttttaaatcg
     1741 ttgagcaagg ttgggttggc caagtattgc ttgttcaatg caatttttgc aggcataatt
     1801 tcttcaacgt atgttttatg aggattgtcg gattttccca ataattcatt ttcttcaaaa
     1861 taatgcaaag aggcaatgtc tgtaattcct ggtcgcacat tcaaaatttc tcgttgctcg
     1921 ggtgtgtata aatcaacgta ttcttttact tctggacgcg gacccacaat actcatatcg
     1981 cctttaatta cattgatgaa ctgtggaaat tcatccaact tatatttacg aatgaaatag
     2041 cccgttctgg tgattcgagc atcgcgcatt cctacggtta attttccttg tttatctgca
     2101 tctttgcgca tagagcgaaa tttaaacaac aaaaacggtt gaccaaatct tccaatacgt
     2161 tcctgacggt aaaaaacgcc tcctttgctt tctaaaacaa ttaaaagcga caaaatcaat
     2221 ccgaagggta aaaacaacga aagaataatc agcgagcaaa aaatgtcaaa aatgcgtttc
     2281 atgaaagttt aaatatcgcc caaaagtaag atttattttt ttgtactttt gtagtatgaa
     2341 aataaaatca gctgtttttg cagtttcaaa caccgattat aagaaatgtc caacaggaga
     2401 tttgcccgaa tatgcattca ttggccgctc caatgttgga aaatcgtcgt taatcaacat
     2461 gttaaccagc caaaaaaact tggcaaaaac atcaggaaaa ccagggaaaa cgcagttaat
     2521 caatcatttt ttaatcaacg aaaattggta cattgtggat ttgcccggtt atggttatgc
     2581 caaagtagca aaatcgcaac gcgaaaaatt tgagaaattc attgccgaat acttgctcaa
     2641 ccgccagcaa ttggtcaatg tgtttgtatt agtggattca cgcttagacc ctcaacaaat
     2701 tgatttggaa ttcatgaatt ggtgtggtga aaaagaaata ccgttttcca tcgtttttac
     2761 aaaaatcgac aaactttcgt cttctgcttt gcaaaagaac atggcaaaat ataaaaagga
     2821 attgcaaaaa tactgggcag aaatcccacc tgtgtttact tcttcaagtg agacaggttt
     2881 tggaagtgaa aaaattctta attacattga acaaatcaat caagcaattg ctggaaatca
     2941 ataaaaaatt agtaatttca acgataaaaa aaattcaaac atgatcatcg aacaattata
     3001 taccaaatgt ttggctcaag gagcttattt catttctcat aaaggcgaag cagccgttat
     3061 tgaccctatt cgtgaggtgg accaatacat tgaattggca aaagcaaaca atgctaccat
     3121 caaatatatt tttgaaacac attttcatgc cgattttgtt tcgggacacg tggatttggc
     3181 tcgcaaaaca ggagcaacaa ttgtttacgg gccaacagcc aaaaccaatt ttgattgctt
     3241 ggtagctacc gataatcaag aatttaaaat tggcgattta accattcgtt tgttgcacac
     3301 acctggtcat acattagaat catcctgcta tttgctattg gatgaaaacg gtaaagaaat
     3361 tgccttgttt tcgggcgaca ccttgttttt aggtgatgtg ggtcgaccag atttagccat
     3421 caaaagcgat ataacaatgg aggatttggc tggaatgtcg tttgactctt tgcgcaatcg
     3481 aatcatgcct ttgccagatg acgtgatggt ttatccagga cacggagctg gttctgcctg
     3541 tggaaaaaac atgagcaaag aaacacaaga tttattgggc aatcaaaaac gcaccaatta
     3601 tgccttacgt gccgacatga cgcgtgatga atttatcaaa gaagtaacag aaggtatctt
     3661 acctccacct caatattttc ctaaaaatgc attgctcaac aaaacaggtt atgaatcggt
     3721 tgatgaggta attgcacgtg gttcacaacc tttatcgttg gcagaattta aacaaaaaat
     3781 tgccgaaggt gctttgattt tggatgtgcg caatcaaaat gattttgtgc aaggatttat
     3841 tccaaattca ttgtttattg gcattgacgg tcaatttgct gtgtgggttg gaaccttgat
     3901 tcaggatatc aaacaaccaa ttgtagtggt ttgtccagaa ggtaaagaaa cagaagtggc
     3961 tacacgtttg gcacgtgttg gatacgacaa tgctttgggt tatttaaaag gcggtgttgc
     4021 tgcttgggac ggtgaattga ataccatcca aaatgtagag gcaactgcat tagaatcggt
     4081 tgatgcacag tcgattgttg atgtacgcaa gccgggtgaa tacaacgcag aacatgttga
     4141 aaactgtgtg ttctatccgt tggattttat gagcgatgat ttgaacaaac tcgacaaaga
     4201 aaaaccgttg tatgtgcact gtgctggtgg ttaccgtagt gttattgcca tttcattgtt
     4261 gatgcaaaaa agcaattatc gcaacttaat caatgtggtt ggcggatatg ctgcaattgc
     4321 aaaaacagct gtacctaaaa cagcatttgt ttgttcttct aaataataat caatgaaaaa
     4381 caacgaaata gctgtaaaaa cggcctattt cagcatcttt agtaattttt gtttggcatt
     4441 aataaagggt gttgcgggtt ttttcggaaa ttcctacgcc cttattgccg atgccattga
     4501 atcaacaact gatgtgtttt cgtctatttt agtgttgttg ggcattcgtt attccagtcg
     4561 cccagccgat gaaaaccatc cctatggtca cggcaaaatt gaaccgctcc ttacctttgt
     4621 tatcgttgga tttttaatta cttctgctgt ggtaattgcc tacgaaagtt gtaaaaatat
     4681 ttttacacca catgctttgc caaaatcatg gaccttatac gtgttgggag caataattgt
     4741 atggaaagaa gcatcgtatc acattgtact ccataaaagc aaacaaatca acagcaccgt
     4801 attaaaagca gatgcttggc accatagaag tgatgctatt acatcaattg cagcgtttat
     4861 tggaatttcc atcgccttgt tttttggaaa aggctacgaa gccgccgatg attgggctgc
     4921 cctgctggct tctgttttta tactttacaa cagctaccgc atttttcgtc ctgctttgag
     4981 tgaaattatg gacgaaaaca cctatgaaga catcattaaa caagtgcgtg cagttgccac
     5041 aaccgtagat ggcgtaattg ataccgaaaa atgctatgtt cgcaagtcgg gagtttacta
     5101 ttacattgat ttacatgcaa ccgtggatgg aaatgcaacc gtaaaaaaag gacacgatat
     5161 ttcccatcga cttaaagatg ccttgcaaag caacttaccg ttcgtcaaag atgtgttgat
     5221 tcacattgaa ccacacgata ttggggagga agtaaattta gattaaacca atccgttttt
     5281 taccaacaat tttgcaattt gcaccgcatt ggttgcagca cctttgcgca agttatcggc
     5341 aacaatccac atgttgagcg tgtttggttg cgattcgtct ctgcggatac gcccaacaaa
     5401 tacatcgttt ttaccttctg cataacgagg cattggataa atattggtaa tagggctatc
     5461 tttaaccact acgccggcag ttttgtgtaa aatagtgcgc acatcgccta aatcaaaatc
     5521 gcgactaaat tctacgttaa cagcttctga gtgaccacca accacaggaa cacgcacagc
     5581 agtagctgta acattaattg caggatctaa aatctttttt gtttcgttgg ttagcttcat
     5641 ttcttctttg gtatacccat tgtccaaaaa gctatcgcaa tgtggcaaac agtttttgtc
     5701 gataggataa ggataaacca tagaagcggc tacaccattg cgctcatttt ccatttgttc
     5761 aaccgctttc acacctgttc cagtaatgga ttgataggta gaaacaataa cacgttttac
     5821 ggtataaaca cggtgcaatg gagccaaagc catcactaat tgaacagtgc tacagttagg
     5881 attggcaata attttatcgt cttttgtaag ttgatctccg ttgatttctg gaacaatcaa
     5941 tttttttgtt gcatccattc tccatgccga tgaattgtcg ataaccgttg tgccaacagc
     6001 tgcaaattta ggcgcccatt ccaatgaggt ttcgccacca gccgaaaata aagcaattgc
     6061 cggtttcatg tcaacagcag tttgcaaatc aactaccgca tactcttttc cagcatattc
     6121 tactgttttt ccaaccgatt tttcagaagc aacaggaatt aattcgctaa caggaaaatt
     6181 ttcttctctc aacacttgta gcataacttg accaaccatt ccggttgctc caacaactgc
     6241 aattttcata acctacgatt ttgtttttat tattcaagcg aaattactac tttagcaaga
     6301 ataaatacta tttctaccaa tgaaatttgt taaatatcta aaaccgaaaa caactttttt
     6361 gcaaacagtt atgttcttgc tattccaagt tccaactgtt gcattgtctc aaataactga
     6421 tgattttaat gacggcgatt tttccaataa cccaacatgg ttaggaacag ttgcagatta
     6481 tcaagtgaat actagtcaac aattgcaatt aaatgcagca gttgcaggca catcgtattt
     6541 atatttaccg cataatttaa ccgatttcaa cggaaaagaa tggcgttttt gggtgcgtca
     6601 aaattttgct ggttcggcaa acaattatgg aagaatttat ttaatcgcct cagccacaga
     6661 tttatccacc gatccagatg gagtttacct tcaaatgggc gaggcaggtt caacggatgc
     6721 tgtgcgttta atgcaacgaa acggaggagt aacaactacc atttgtgcat ctgctgatgg
     6781 aagtattgca aatgcctttg ccataggtgt aaaaatcatt cgcgataata ccggaaattg
     6841 gtcactaaac attgattttg caggtggagt taattattca gctattgcaa caggagttga
     6901 aacaacggta ccaacaggcg catattttgg ttacttgaat gtttatactt ctggaaatat
     6961 caccaaattt tatttagacg atgtatatgc gggagatcaa gtgtttgata caacaccacc
     7021 agttatgaca tcggtacaag ttattagcaa caaccaattg gatgtattgt tttccgaagc
     7081 tgtaacagca acatccgctc aaacgattaa taattacgat ctcataccaa tcaacagtgt
     7141 gagttcggca actttagacg gaactaaccc agcattggtg catttgcaac taactacacc
     7201 tttggtgaat ggaaatactt acaccttgtc gagcaatgca attgaagact tgagtggaaa
     7261 cattgcctct aatcaatcgt tgaattttac ttatttggtg gcagaaactc ctttggctgg
     7321 tgatgtgttg attaatgagt tcatggccga cccttcgcca gtagttggtt tgcctgaagt
     7381 agaatttgta gaaatttaca acaaaagcaa taaagtgttc aatgtcagtg gatggaaatt
     7441 aggcgataat gcaacctttg gaaccattac ttctggatgg ttgttgccca atcaatacct
     7501 tgttttatgt ccaaatgcca atgttgccga atttaccaat gcggtaggtg taaccagttt
     7561 tccaagttta aataacgcag cggatgacat tgtaattcaa gacaatacag gtgtgcaatt
     7621 ggataaaatc agctatactg atgcgtggta ccaaaataca gcaaaaaaag atggcggttg
     7681 gaccattgag cgcattaacc ctaatgaacc ttgttcacaa gcaagtaatt ggaaggcttc
     7741 aactgctgta gcaggaggaa caccaggcgc acaaaattcg gtgcacgatg taacacccga
     7801 ctcgcaagca gcacaaattg ctggaatcaa cattctgtca accaaccaaa tagaggtggt
     7861 gtttaacaaa atggtagatt caacttcact taaaaatgca gcaattacgg tcaatccaac
     7921 gctaactgaa aacaaccgaa ccattgcaag tatccacgtt gcttcatttt cgatggattt
     7981 tgtagaaaat tttgcagaag gccagtttta caccttgagc attccatcag ttgttgattg
     8041 ttggggaaat acgggggatg tgagcgcaac ttttgtttta ccttcgcaac ccgatagtgg
     8101 cgatgtggtc atcaatgaaa ttttggtaaa ccccattact ggtggtagcg attttgtgga
     8161 gttgtataat aaatccgata aattgctgga tataaaaaat tggaagatag cacgaattta
     8221 caatggcgtt gtaacagatt ataaaacaat tactaacagc cgtttacttc atccaaaaga
     8281 ttatctggtt gtaactgctg atagcaattt tgtgatgagc aattatcctg caacaatacc
     8341 caataaattt tatgcattga ctttacctac actttccaac gatacaggaa gtgtggtatt
     8401 gacctacgat aaatttgtgt ttatggatac tatagccgtt gtgctcgatc aattggtgta
     8461 ttccagtaaa tggcatttta aactcataga tgataacaaa ggaaaaacct tggaacgatt
     8521 agaccccaac cggccaacgc aagatgcaag caactggcat actgctgccg aatcgattgg
     8581 gtttggaacg ccaggcggaa aaaattcaca atactatcct gccattcacg atggggaagt
     8641 gagtttatcc agtgaaacca tttcacccga taacgatggg tttgaagatg tgttgttgat
     8701 taactatacc atgacttcac ctggtatggt ggcttcgttt tatgtgtacg atggtgcagg
     8761 aagaaaagta agaaccattg ctcaaaacga attgttggga atggaaggaa cgattgtttg
     8821 ggatggtgtt cgcgatgatg gtcaaaaagc attgataggc acctatgtgt tggttgttaa
     8881 tgcgtttgca accgaaggag gtaagaaatt tgtggctaaa aaaccgtttg ttgtagcagg
     8941 gaagctgtaa aacacacctt ccccctcaac aattttttat tttata
//
LOCUS       DAOQVQ010000045         8974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8649_length_8974_cov_4.276713, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000045 DAOQVQ010000000
VERSION     DAOQVQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8649_length_8974_cov_4.276713"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..784)
                     /locus_tag="PKN22_02510"
     CDS             complement(92..784)
                     /locus_tag="PKN22_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS41605.1"
                     /translation="MEKKLAILLAEDDENLGLLLTTYLTSKGFKVTLKRDGQAALEEF
                     NSTKFDFCVFDVMMPKMDGFSLAKEIRELDKKVPILFLTAKDMKEDKLKGFDLGADDY
                     LTKPFSMEELIARISAIARRAIGDSTEQSGDIYIGTIKYEPELRLLYTKEEVKKLTTK
                     ENQLLNLLVKNKNEILDRQATLRAVWGDDNYFNGRSMDVYIAKLRKLLKDDENIEIMN
                     VHGKGFKLLIKE"
     gene            complement(787..2391)
                     /locus_tag="PKN22_02515"
     CDS             complement(787..2391)
                     /locus_tag="PKN22_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS41606.1"
                     /translation="MGKIRVNVIIVIVVAAILALLLIQAYQTKQMFDKKSHQFADQVQ
                     TTLERIAVRHEKAEDVRHFFTIANKNFNGHYKEILKEEFQNLIAPKETISIKDTMIWE
                     NGKQENYLLVQGQSFDSLIGLKAEQRVLAKDVRQLRDFYQGKTPKDSVGVAIHLNRDV
                     VKKMFEKARFVNDIMMQSFKESLYETPGNVFDIAFLDSVIRTELKDDKLPAKFEFVVV
                     NESKKPIVCHSKIEQYNAKLDTTNAFATHLFPSNLFDEKLTLYVDFTLKNGFLLKELW
                     FPLLISILLVSLIFWVLIYLFQTILTQKKLSEIKNDFISNMTHEFRTPISTISLACQA
                     MNDDDVVGLVDTNVQPFIRMIDNENQRLSVLVDSILQSSTMDKGEIHLNKEKVLVNEV
                     IYDVVHRAQLRLQNTNGKININIGVELIYIEADKLHFTNTISNLVDNAIKYSEDEPNV
                     TITMVSTGNSIQISVADKGIGIKKEHLNKIFDKLYRIPTGNVHNVKGFGLGLSYVKGI
                     CQMHGWKISVASKYGEGSTFTIEIKK"
     gene            complement(2518..2874)
                     /locus_tag="PKN22_02520"
     CDS             complement(2518..2874)
                     /locus_tag="PKN22_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41607.1"
                     /translation="MKNLFSALIFILLVGFSFGQASVKTASDLTSIKEKGVGYITLPS
                     NLTKEDVASRAKYYPLYFTVDFNESTKVATIKMVENDERSRAIIVRFLAGCEVQTINV
                     AGETISRDQLFEKYLK"
     gene            2968..3570
                     /locus_tag="PKN22_02525"
     CDS             2968..3570
                     /locus_tag="PKN22_02525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNS41608.1"
                     /translation="MSLEVCHTLGIFEAGCDEAGRGCLAGPVVAAAVILPDDFSNALL
                     NDSKKLTDKQRYSLRPIIEKEAIEWAVGIVDNVEIDEINILNASFLAMHRAVDQLKTR
                     PQQLLIDGNRFKVYKDIPHLCMVKGDGRFMNIAAASILAKTYRDDLMDQLHEEFPHYG
                     WKKNKGYPTAEHRKGIEEHGATPYHRMSFTLLASNQLSLF"
     gene            3653..5800
                     /locus_tag="PKN22_02530"
     CDS             3653..5800
                     /locus_tag="PKN22_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41609.1"
                     /translation="MFGRIFLGIVAIISLLWLGYATYDRVNNDIQYTVEYLFNDEDGA
                     VLIVHQPDQLESLIADFKLNASNKVGAVLQTLATDKIASVAISQNRNQLLIQTKKEIT
                     PTSVSTLFNNASSLKINQKKLEILGLKGSFQNNFIYLATEDYEHNASDWSAVQYDKNA
                     AGSFINLSGNQYAVTDIYLKNGGIVEYKFQAKKTINATKVNDQVVFANVVPTSSTIYE
                     FYETDYLRHKFPEIMKNPINNWLRYGLVKVKVNGNEAVITDYIDGQDPIQVLYDFYKK
                     ESKVADNDYFANAQLPNVLNAKGLYVYRFDDYVVLANNRAICETIIGDYKLGKTISQN
                     PQKLSDVYNQLPQKVNYRKVDTTTKFATSVYKNVLLTTVVRGEVTLNATEKETTDSSN
                     SFVVGGVASDIQFIHENAFFVTTKENKVIFFDNGKKIWEQTVDGELVGNGTLIDLYAN
                     QKNQLLVATNKKIYVFDINGAQPNGFPINLDDQTLIQQPVLYRWKGNGVFIAPLTGGR
                     IGQFDNQGRELAIIRTKLNEITAPPIVWVSANKPFIGVTDGVQFEMIQADIRKSYRVF
                     ATNEAKLLLKLPNEIKLFGILKNQLVAYNQKGGVSYFEQFSNGTLLPTLQPELGIVVK
                     DGQTIKLFNSNGVQWGAIPLSFSDVADVQIFNTTNGATFIAVVDGLENKVHLYKSNGA
                     LFKEQPLEGSKMVRYYNGHLFTVIDNLIVKYPL"
     gene            5797..6744
                     /locus_tag="PKN22_02535"
     CDS             5797..6744
                     /locus_tag="PKN22_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS41610.1"
                     /translation="MIEEHSDYFLSNNCDSNEQHRLFYSQFVPSDKNSIKATLLIVHG
                     MQEHCGRYTDFARFLAHNGIAVLAYDHLGHGKTAQGSADFGYFGKNGRKQVVADAQNI
                     ANFLGKQFPTVPHFIFGHSMGSFITRCLLQIAHDQFEGAIICGTGGKIPGIQLAKALT
                     ATLNFVAPKSKSTFIEKVFGNMNNARFKAEGNQNNTNWLSVDTENRKAFLADELCGIP
                     FTNNGFDTLIALNRDATKRSWASAIRKNLPLLFISGEDDPIGNFGKGVRQTVANLKTD
                     GFDNVTMQLYPKKRHEILNEANKQEVYTTVLQWLEKTKG"
     gene            6801..7931
                     /locus_tag="PKN22_02540"
     CDS             6801..7931
                     /locus_tag="PKN22_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNS41611.1"
                     /translation="MSKLPHVGTSIFSVMSKMATEHQAINLSQGFPNFPIDSQLTEIH
                     SRIVKEAQSNQYCPMAGHPQLLEEIQRLIFKSYGRMVDAAAQLLVTAGATQAIYTIFQ
                     ALVEKGEEVIVLDPCYDSYEPAILMAGGKPIHVALDNDYLPDWNKIEQAITPATKFLV
                     INNPHNPSGRVWSEKDYEILEALIEKHPRLLVLSDEVYEYITFEKKHLSINERPQLHQ
                     RALSVSSFGKTFHITGWKIGYIVAPQPLMDEIKKVHQFLVFCVNSTAQATLAAYLKEV
                     DVTQLGAFYQQKRDFFQSLIKNSRFELLPSEGTYFQLASYANISQEDDVAFTKRILME
                     HKIATIPVSVFNSDGRDLKHIRLCYAKTEETLIQAAEMLNKI"
     gene            7938..8711
                     /locus_tag="PKN22_02545"
     CDS             7938..8711
                     /locus_tag="PKN22_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS41612.1"
                     /translation="MEKLIISLVQANQIWEDKAANYQNYNRLLGSIPSADLVVLPEMF
                     STGFSMNAANLAEEMESSTAIEWLKQVSLAKNTAIYTSLIIKENGHFYNRGVFVAQGE
                     VVAHYDKRKTFGLAGEDKIYTAGNTPVIVNYKGWNIRLQICYDLRFPEIALNKITDGQ
                     PEYDLLLYVANWPKRRNVHWNTLLPARAIENQCFVAGVNRVGTDANGLTYSGDSVVYD
                     LLGQQLCYSAHQEQVLTCELSKKELLDNREKLPFLKDRE"
BASE COUNT         2898 a         1647 c         1604 g         2825 t
ORIGIN      
        1 aagctatttt ttaaaacata aaaaatggcc ggaatttctt ccgaccattc gtgacggata
       61 acccgtcctt ttttgtttta taacaatgct attattcttt aatcaacaac ttaaaacctt
      121 taccgtgcac gttcatgatt tcaatgtttt catcgtcttt taacagcttg cgcaattttg
      181 caatgtacac atccatgctt cggccattaa aataattatc atcgccccat acggcacgca
      241 aagtagcctg acggtctaaa atttcgtttt tgttttttac caataagttc aacaattgat
      301 tttcctttgt ggtcaacttc tttacttctt ctttggtata cagtaagcgc aattctggtt
      361 cgtattttat tgtgccaata taaatatcac cgctttgttc ggttgaatcg ccaatagcac
      421 gtcttgcaat tgcactaatg cgcgcaatca actcttccat tgaaaaaggt ttggttaagt
      481 aatcatcggc accaagatca aaccctttta atttatcttc tttcatgtct ttggctgtca
      541 agaataaaat tggaactttt ttgtccaact cacgaatttc tttcgcaagt gaaaatccat
      601 ccatttttgg catcatgaca tcaaatacac aaaaatcaaa tttagtactg ttaaattctt
      661 ccaaagcagc ctgaccatca cgtttaagcg ttactttaaa tccttttgat gttaaataag
      721 tagtaagtaa caaacctaaa ttttcatcgt cttcggctaa taaaatagct aatttttttt
      781 ccataattat ttttttattt caattgtaaa agttgagccc tccccgtatt tactagcaac
      841 actgattttc catccgtgca tctggcaaat tcctttcaca tagctcaacc ccaatccaaa
      901 acctttcaca ttgtgaacgt ttcctgtagg aatacgatat aatttatcaa atattttatt
      961 taagtgttct ttttttattc caattccttt atcagccacc gaaatttgaa tgctatttcc
     1021 tgttgaaacc attgttatcg ttacgtttgg ctcatcttct gaatacttaa tggcattgtc
     1081 cactaaatta gaaattgtat tggtaaaatg caacttatct gcttcaatat aaatcaattc
     1141 cacgccaata ttgatattta ttttaccatt ggtgttttgt aaacgcaatt gcgcacggtg
     1201 aaccacatcg taaataacct cgttgaccaa tactttttct ttgttcaaat gaatttctcc
     1261 tttatccatc gttgaacttt gcaaaatact atccaccaac acacttaatc gttggttttc
     1321 attatcaatc attcggataa aaggttgtac attggtatca actaaaccta ccacatcatc
     1381 atcgttcatt gcttgacatg ccaatgaaat tgtagaaatt ggtgttctaa actcatgggt
     1441 catgttggaa ataaaatcgt ttttaatttc agataatttc ttttgggtta aaatggtttg
     1501 aaacaaataa atcaataccc aaaaaataag cgaaaccaac aatatactga tgagcaatgg
     1561 aaaccacaat tctttcaata aaaatccatt ttttagtgtg aaatcaacat acaaagtcaa
     1621 tttttcatca aacaaattgc tgggaaataa atgagttgca aatgcgttgg tggtgtctaa
     1681 tttagcattg tattgttcaa ttttagagtg gcaaacaatg ggttttttcg actcgttgac
     1741 caccacaaat tcaaatttgg caggcaattt atcgtcttta agctctgttc gaattactga
     1801 atctaaaaaa gcgatatcaa acacatttcc aggagtttca tacaagcttt ctttgaacga
     1861 ttgcatcatg atgtcgttta caaatcgtgc tttttcaaac atttttttta ctacatctcg
     1921 attgaggtga atggcaactc caacagaatc ttttggggtt tttccttgat aaaaatcacg
     1981 cagttgacgc acgtcctttg ccaacacacg ttgttcggct ttcaatccta tcaatgaatc
     2041 aaaactttgt ccttgtacca aaaggtagtt ttcctgtttt ccattttccc aaatcatggt
     2101 gtctttgatg gaaattgttt cttttggggc aattaaattt tgaaattctt cttttaaaat
     2161 ttctttataa tgaccgttaa agtttttgtt ggcaatggta aaaaaatgac gtacatcttc
     2221 cgctttttca tggcgaacag caatgcgctc taatgtggtt tgcacctggt cggcaaattg
     2281 atgcgatttt ttatcaaaca tttgttttgt ttgataagcc tgtatcagca acaacgccaa
     2341 aatagctgca actacaataa caatgataac atttacccta atttttccca ccttctattt
     2401 tttaacaaaa ttactgcttc ctattgctga aacagccgag cttaacgttt attaacactc
     2461 acaaaaaaag tcggaagttt gggctcccga cttagaactt agtatttttt aatctgctta
     2521 tttcaaatat ttctcaaaca attgatctct ggaaatagtt tcgccagcaa cattgattgt
     2581 ttgaacttca catcctgcta agaaacgaac aatgatagca cgtgaacgct catcgttttc
     2641 taccatttta atcgttgcaa cttttgtaga ttcattaaaa tctacagtga aatacaaggg
     2701 ataatatttt gctctagagg ctacatcttc ctttgttaaa ttggatggca atgtaatgta
     2761 accaactcct ttctccttaa ttgacgtcaa atcactagca gttttaacac ttgcttgacc
     2821 aaatgaaaat cctactaaaa ggataaaaat aagtgctgaa aataaatttt tcatcttttc
     2881 taattttaaa agttagacgt taaatagtac tcaaaagttg taccaatata cgataatatt
     2941 ttaacttagt tccgttttaa agaaaaaatg tcattagaag tttgtcatac ccttggaatt
     3001 tttgaagctg gttgcgatga agctggtcgt ggttgtttgg ctggacccgt tgttgcagcc
     3061 gcagtcattt tacccgatga tttttcaaat gcattgctca acgattctaa aaaactaacc
     3121 gataaacaac gttattcact tcgtccaatt attgaaaaag aagccattga atgggctgta
     3181 ggaattgtgg ataatgttga aattgatgaa attaacattc tcaacgctag ttttttagcc
     3241 atgcatcgtg ctgtcgacca attaaaaaca cgacctcaac aattgctcat tgatggcaat
     3301 cgatttaagg tctataaaga catcccacat ctttgtatgg tgaaaggcga tgggcggttt
     3361 atgaacattg cagcagcatc aattttggct aaaacatatc gcgatgattt aatggatcaa
     3421 ttgcacgaag aatttccgca ctatggctgg aaaaaaaata aaggttatcc aactgcagaa
     3481 catcgcaagg gaattgaaga acatggcgcc actccctatc accgaatgag ttttaccttg
     3541 cttgcctcca atcaattgag cttgttttaa ggggctcaac ctaacaatta actgttgatt
     3601 tctttaaatc actcttgttt ttaaggctct aattccttgt aaatttgaaa ctatgtttgg
     3661 tagaattttt cttggaattg ttgcaattat cagcttgttg tggttgggtt atgcaacgta
     3721 tgaccgtgtt aacaacgata tacaatacac cgttgaatac ctttttaacg atgaagatgg
     3781 tgctgttttg attgtgcatc aacccgatca acttgaaagt ttaattgctg attttaaact
     3841 aaatgcctct aacaaagttg gtgctgttct tcaaactttg gcaactgaca aaattgcttc
     3901 cgttgctatt tctcaaaata gaaatcaact gttgattcaa acaaaaaaag aaataactcc
     3961 aacgtctgtt tctaccctat tcaataacgc atcatcgtta aaaatcaatc aaaaaaaatt
     4021 agaaattctc ggattgaaag gaagttttca aaataatttt atttacctcg ccactgaaga
     4081 ttatgaacac aatgcaagcg attggagtgc ggtgcaatac gataaaaatg ctgctggttc
     4141 gtttattaat ttgtcaggaa atcaatatgc agttaccgat atttatttga aaaatggtgg
     4201 aattgttgaa tacaaatttc aggcaaagaa aacgatcaat gcaacaaagg taaacgacca
     4261 agtggtgttt gcaaatgtgg ttcctacatc cagtacaatt tatgaatttt acgaaaccga
     4321 ttatttgcgc cataaatttc cagaaataat gaaaaaccct atcaacaatt ggttgagata
     4381 tgggttggta aaagttaaag tcaatgggaa tgaagctgtt attaccgact atattgacgg
     4441 tcaagatcct attcaagtgc tgtatgattt ctacaaaaaa gaatctaaag tggccgataa
     4501 cgattatttt gcaaatgcac aattgcccaa cgtgttgaat gcaaaaggct tgtatgtata
     4561 tcgatttgat gattacgttg ttttagcaaa caatagagct atttgcgaaa caattattgg
     4621 cgattacaaa ttgggaaaaa caatttcgca aaacccacaa aagttgagcg atgtgtataa
     4681 tcaattgcca caaaaagtca attaccgaaa agtagataca accacaaaat ttgcaacttc
     4741 tgtctataaa aatgtattgc taacaactgt tgttaggggc gaagttacac tcaacgcaac
     4801 cgaaaaagaa accaccgatt cttcaaactc ttttgtagta ggcggtgttg cttcggatat
     4861 ccaattcatt catgaaaatg cgttttttgt aacaacaaaa gaaaacaagg tgattttctt
     4921 tgacaatggg aagaaaattt gggaacaaac ggttgatgga gaattggtgg gcaatggaac
     4981 cctcatcgac ttgtatgcca accagaaaaa tcaattgttg gtggctacta ataaaaagat
     5041 ttacgtgttt gatatcaatg gtgcgcaacc caatggattt cctatcaatt tagacgatca
     5101 aacgttgatt cagcaacctg ttttgtaccg ctggaaagga aacggcgtat tcattgcacc
     5161 attaacaggt ggaagaattg gtcagtttga caatcaaggg cgcgagttgg caattattcg
     5221 cacaaaacta aatgaaatca ccgcccctcc tattgtttgg gtaagtgcaa acaaaccatt
     5281 tattggtgta acagatggtg tgcagtttga aatgattcaa gccgatattc gcaaatctta
     5341 tcgtgttttt gcgacaaatg aagcaaaatt gttgttgaaa ttgccgaatg aaattaaatt
     5401 gtttggtatt ctcaaaaacc aattggtggc ctataaccaa aaaggaggcg tttcttattt
     5461 tgaacaattt agcaatggaa ccttgttgcc aactttgcaa cctgagttgg ggattgtcgt
     5521 aaaagacggg caaaccatta aactgtttaa ctcaaacgga gtgcaatggg gtgctattcc
     5581 attatccttt tcggatgtag ccgatgtgca aatttttaac acaacaaatg gcgcaacgtt
     5641 tattgcagtt gtggacggat tggaaaacaa agtgcattta tacaaatcaa acggagcatt
     5701 gtttaaagaa caaccgttgg agggaagtaa aatggtgcgt tattacaacg ggcatttatt
     5761 tactgtaatt gataacttaa ttgtcaagta tccattatga ttgaagaaca ttctgattat
     5821 tttttgagta acaactgcga cagtaatgag caacaccgtt tgttttattc gcaatttgtt
     5881 ccttcggata aaaacagcat taaagcaacg ttgttaattg ttcacggcat gcaagaacat
     5941 tgtggacgtt ataccgattt tgcacgtttt ttagcacaca atggaattgc agtcctggcc
     6001 tacgaccatt tgggacatgg aaaaacagca caaggttcag ctgattttgg ctactttggt
     6061 aaaaatggtc gcaagcaagt ggtggcagat gctcaaaaca tagctaattt tttaggaaag
     6121 cagtttccta ctgttccgca ctttattttt ggtcattcca tgggttcctt catcacgcgc
     6181 tgtttattgc aaatcgcaca tgatcaattt gagggagcca ttatttgtgg tacaggcgga
     6241 aaaattccgg gcattcaact agcaaaagct ctaactgcta cgcttaattt tgttgcgcca
     6301 aaaagcaaaa gcacctttat tgaaaaagta tttggcaaca tgaacaacgc tcgttttaaa
     6361 gcagaaggta atcaaaacaa caccaattgg ctcagcgttg acacagaaaa tcgcaaagca
     6421 tttttagcag atgaattgtg cggcattcct tttaccaaca atggatttga cacgttgatt
     6481 gccttaaaca gagatgccac aaaaagaagt tgggcaagcg ctattcgcaa aaatcttccc
     6541 ttgttgttta ttagtggaga agacgatcca attggtaatt ttggcaaagg tgtacgtcaa
     6601 acggtagcca atttaaaaac cgatggattt gataatgtta caatgcaatt gtatcctaaa
     6661 aaacgccatg aaattctcaa cgaagctaat aaacaagaag tttacaccac tgttttgcaa
     6721 tggttggaaa aaaccaaagg ttaatcgcct acattgaatt gcgttaattt tattatattc
     6781 gttcaacata attagaacgc atgtcaaaat taccccacgt tggaaccagc atttttagtg
     6841 ttatgtctaa aatggcaacc gagcaccaag ccatcaattt atcgcaagga tttcctaatt
     6901 ttccaataga ttcacaactg accgaaattc atagtcgcat tgttaaagaa gcacaaagca
     6961 atcaatattg ccccatggct ggtcacccac aattgttaga agaaattcaa cggttgatat
     7021 tcaaatccta tggcagaatg gttgatgctg ctgcgcaatt attggttaca gctggtgcca
     7081 cgcaagctat ttataccatt ttccaggcct tagtagaaaa aggcgaagaa gtaattgttt
     7141 tagatccttg ctacgattcg tatgaaccag caattttaat ggcaggcgga aaaccaattc
     7201 atgtggcgtt ggacaacgac tatttgccag attggaataa aattgaacag gcaataactc
     7261 cagcaaccaa atttttagtc atcaacaatc cgcacaatcc atctgggcgt gtttggagcg
     7321 aaaaagatta tgaaatttta gaagcattga ttgaaaaaca cccgcgctta ctcgtgcttt
     7381 ctgacgaagt gtatgaatac ataacttttg aaaaaaaaca cctatcaatc aatgaacgcc
     7441 ctcaattgca ccaacgagct ttgtcggtta gttcgtttgg aaaaaccttt cacattactg
     7501 gttggaaaat tggctacatc gtggcaccac aaccgttgat ggacgaaatc aaaaaagtac
     7561 atcaatttct agtattttgt gtcaacagta ccgcacaagc aacattagcg gcctatttaa
     7621 aagaagttga cgtgacgcaa ttgggtgcat tttaccaaca aaaacgcgat ttctttcaat
     7681 ctttgataaa aaatagccga tttgaattat tgccttccga aggaacgtat tttcaattgg
     7741 caagctacgc caacattagt caagaagacg atgtggcatt taccaaacgt attttgatgg
     7801 agcacaaaat tgccactatt cccgtttcgg tattcaacag tgatggtcgc gatttaaaac
     7861 acatccgttt gtgttatgca aaaacagaag aaacgttgat tcaagcggca gaaatgttga
     7921 ataaaatata atctaccatg gaaaaattga tcattagttt ggtgcaagcc aatcaaattt
     7981 gggaagataa agcggctaat tatcagaatt acaatcgttt attgggaagc attccctccg
     8041 ccgatttagt ggtgttgccc gaaatgtttt cgactggttt ttcaatgaat gcagccaatt
     8101 tggccgaaga aatggaaagt tcaacggcta tagaatggtt aaaacaagtc tccttagcta
     8161 aaaacaccgc catttacact tcattgatta tcaaagaaaa cggacatttt tacaaccgtg
     8221 gtgtttttgt tgcccaaggc gaagtggtgg cacattacga taaacgcaaa acctttggat
     8281 tggcaggaga agataaaatt tatacagcag gaaatacacc cgtaattgtc aactataaag
     8341 gatggaacat tcgtttgcaa atttgctacg acttgcgttt tccagaaata gcattgaaca
     8401 aaatcacaga tgggcagcca gaatacgacc ttttgcttta tgttgccaat tggcccaagc
     8461 gaagaaatgt gcattggaac accttgttgc cagcaagagc tattgaaaat caatgttttg
     8521 ttgcaggtgt taaccgtgtt ggtacagatg ccaacggttt aacctattcg ggcgatagtg
     8581 ttgtgtatga tttactgggt caacaattgt gttacagtgc acaccaagaa caagtgttga
     8641 catgtgaatt atctaaaaaa gaacttttag ataatcgaga aaaattacct tttttaaaag
     8701 atagagaata gacagttgcg agtagaaaac aggtagaata ttttaaaaaa tacctctctt
     8761 ttttcgcaaa aattgtactt tcgttcaata aattgattaa aaacaaagaa aaaacacact
     8821 atgagtgaca accgcaaaca tgtagaagtt tgttttacac caggagaata cgattatttt
     8881 caaccaggtt atgaaattgt tgttgtaatt gacgttttgc gtgctacatc ggcaatttgc
     8941 gctgcttttg acaacggtgt aaagtccatc attc
//
LOCUS       DAOQVQ010000046         8974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8650_length_8974_cov_3.692118, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000046 DAOQVQ010000000
VERSION     DAOQVQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8650_length_8974_cov_3.692118"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKN22_02550"
     CDS             complement(<1..214)
                     /locus_tag="PKN22_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41613.1"
                     /translation="MKVKQIVELLASTDEKQVKKALNGLKADGDSTVIEPLVQLLQKD
                     AETKLFQKEILEILSSLKDSSSVEAMI"
     gene            292..3672
                     /locus_tag="PKN22_02555"
     CDS             292..3672
                     /locus_tag="PKN22_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNS41614.1"
                     /translation="MEKVTPYIPKNKVRIVTAASLFDGHDAAINIMRRIIQATGCEVI
                     HLGHDRSVEEVVDCAIQEDAQAIAMTSYQGGHLEYFKYMYDLLKQKGAPHIKIVGGGG
                     GTILPSEIKELEEYGITRIYHPDDGRALGLQGMINEVVELCDFPTGQNLTDEVSKLKD
                     KHVPAIAKLISAAENFPEESKAVFAQIHELAAQHQVPVLGITGTGGAGKSSLVDELVR
                     RFLIDFPTKQIAVVSIDPSKRKTGGALLGDRIRMNSIKNERVYMRSLATRQSNLSVSK
                     YVPEALAVLKAANYDLIILETSGIGQSDTEIMDFSDASLYVMTPEYGAATQLEKIDML
                     DFADVIALNKFDKRGALDALRDVRKQYQRNHNLFDLPVDTMPVYGTIASQFNDPGMNS
                     LYKVVIEKVKEKTGADLNSTFQVTKEMSEKIYVIPPDRTRYLSEISENNRSYDKWVNQ
                     QVEVADKLYGLNTSIEALKQSKLDDKDRLIKGLQEAFELEKLNFDPKNWAIIENWATK
                     KDKYKQPKFEFKVRDKVLTLDTHTESLSHTQVPKVALPKFKSWGDILRWNLQENVPGE
                     FPYTSGLFPFKREGEDPTRMFAGEGGPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGN
                     DPATRPDIYGKIGNSGVSICCLDDAKKLYSGFDLTHPLTSVSMTINGPAPMLLGFFMN
                     AAIDQNCEKYIKENGLEAEVEAKIEAIYKKKGVARPRYQGELPEGNDGLGLMLLGVTG
                     DQVLPKDVYEQIKATTLKSVRGTVQADILKEDQAQNTCIFSTEYALRLMGDVQEYFIQ
                     KGVRNFYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYLSRGMDINEFGPNLSFF
                     FSNGIDPEYAVIGRVARRIWAKALDRKYGADARSQMLKYHIQTSGRSLHAQEIDFNDI
                     RTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGLAKNENPLQ
                     GSFIIEELTDLVEEAVLTEFDRITERGGVLGAMETMYQRGKIQEESLYYEMLKHTGEF
                     PIIGVNTFLSSKGSPTVVPKEVIRATEEEKQYQIEMLNNLHQGNKAQTEEGLAKVQEA
                     AIQNKNIFEVLMETCKTASLGQITNAMFEVGGQYRRNM"
     gene            3707..5101
                     /locus_tag="PKN22_02560"
     CDS             3707..5101
                     /locus_tag="PKN22_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNS41615.1"
                     /translation="MKVNTTLGFLFLVILATSCASIKPTAPDFLKEQQQMPSTSVSNQ
                     IVVPLEVNLSGLVKEAENSVPAVMKGGDYPCSGVRYSYEFYKDKFQITADKQVITSKL
                     NGSYWIKMNYCAGCTGLFGDQNCISPVIPFSCGVNETKPSVEIVLSTKIGITKDYALT
                     ATTKIDKVEPIHPCKVTVFRFDATGEVMKRVTAVVESEAKEMDKKLGEIQFKNESEAF
                     WKQLNQSFKIPNLGFLHVQPKAISIVKPRFENNKLYSALLLNCTTFINQEEKNSATSK
                     LPELQVVSSTPKDTFEVFTDITLDYDSLSQLMTTQLGGKTLELRNKQFVFTKFDVNPL
                     PDNRIVIGVHFDGSKNGILYLKGQPVFDNNTKVFSLTAIEYDIETKSSLIKFADWLFS
                     KKITDELQKASTVDFKSDFEKLKAEINQNLKRKIGDYTLSGKVHDAKVHNIYVDLNHL
                     FLRTGVTANLKVTD"
     gene            5155..5667
                     /locus_tag="PKN22_02565"
     CDS             5155..5667
                     /locus_tag="PKN22_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNS41616.1"
                     /translation="MKNYLTIIATAFLFVACNGKQQEDTTAPEVVYSKFGTEITPDKA
                     ISKAEMLEMYKSMKEGDTVKVKFQSEILDVCQKKGCWMTMDLDEENEVFVQFADYGFF
                     VPKNAANHNGIVEGKAFVSITSVDDLKHYAKDAGKSQEEIDAITEPEFSYSFMADGVL
                     LDENLASKSK"
     gene            5669..6103
                     /locus_tag="PKN22_02570"
     CDS             5669..6103
                     /locus_tag="PKN22_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41617.1"
                     /translation="MKAFYWLFFGLLLASCNEKTQNVEENSSKQSPFEMYKISEMAAL
                     MEKMYTNNQAIKQKIEQGNTDLGVFPEEYKTIHVATLTDPSDRDDFFNEHAKKFIAAQ
                     ELVFSESTRQKENFNNMVDACIACHQKKCGGPIVRIKKLYIK"
     gene            6110..6760
                     /locus_tag="PKN22_02575"
     CDS             6110..6760
                     /locus_tag="PKN22_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNS41618.1"
                     /translation="MFFDFLSRLQANNNREWFEANKKQYEQAKSEAAQIFERIYAELI
                     QLDAFEKLKMYSIYRDVRFSHDKTPYKNHFSAIFMRRQPHNRGSFYVHLEPNNSFIGA
                     GFWGPEKEDLLRIRQAIEIEDDLENILKQKELTTSFGAMMGEAVKSAPKGFDKEHPRI
                     HLIRHKQFIFKKSFADNWALQPDFAQQVAADYRILQPFFQYMTDVLTTDANGEPLF"
     gene            complement(6757..7113)
                     /locus_tag="PKN22_02580"
     CDS             complement(6757..7113)
                     /locus_tag="PKN22_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41619.1"
                     /translation="MKTTFLVALSLVFSFVSFSQVVGGDLAKDGRKLTTPSDFTMYES
                     SVGEVYYTISVDINGKVTSAKINGNKSIKVSIPLDVRIYNYLMGPQFEPGTHFPKFHQ
                     GEVKLTLKKKEVKPQP"
     gene            7206..8069
                     /locus_tag="PKN22_02585"
     CDS             7206..8069
                     /locus_tag="PKN22_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNS41620.1"
                     /translation="MDYPASELVLDSQNRVYHLGISPENCAHKIILVGDQDRVALVSS
                     FFDTIEFTHQHREFVTHTGTYKGKRITVLSTGIGTDNIDITVNELDALANINLKNRTK
                     KSNHTALELVRIGTCGILQPTIPVHSYILSEAALGLDNVAHFYNVPFSENEIQECTKI
                     EHHLGLPEKVKPYYIEAAKELVEKFSSPATKNGITITSSGFYGPQGRQLNLSTKTTQL
                     NERLTNYASSDLKVVNFEMESSALFALSKALGHKSTTICLGIANRPKLEFSKDYSEPM
                     NALIVYVLERI"
     gene            8252..>8974
                     /locus_tag="PKN22_02590"
     CDS             8252..>8974
                     /locus_tag="PKN22_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS41621.1"
                     /translation="MSGKKSAGGFSALMGAIAIVIAVIAGILIYKFIFGNPANFVDNN
                     PENEPLQGNFLGIIYKGGFIVPMLVAINLIVVIFTIERFVTLGKAKGKNSLNVFVEKV
                     KAALAGGNIAEARELCEAQKGSLANVVVAGLDKYELMANETSMNKEQKIESVKKELEE
                     ATSLEMPMLTKNLSVISTTASISVLVGLIGTVLGMIKAFAAMSHGAPDTAQLATGISE
                     ALINTALGITGSTVSIIFFNMFS"
BASE COUNT         3035 a         1573 c         1725 g         2641 t
ORIGIN      
        1 ctatcatcgc ttctacagat gaagaatctt ttaaactcga taagatctct aaaatttctt
       61 tctgaaacaa cttagtttct gcatcttttt gtagcaattg caccaacggc tcaatcacgg
      121 ttgaatcgcc atcagctttt aaaccattca acgctttttt cacttgcttt tcatctgttg
      181 atgctaacaa ttctacgatt tgttttactt tcacggtgtt ctcaatttgt taccgcaaag
      241 ttacttatac tctttcacct aacataaata aaaatcacta aatttgtcgc tatggaaaaa
      301 gtaacaccgt atataccaaa aaataaagtt agaattgtta cagctgcgtc tctatttgat
      361 ggacacgatg ctgcaattaa tatcatgcgc cgtattattc aagctacagg ttgcgaagtt
      421 attcacctag gacacgaccg ctcggttgaa gaagtggtgg attgtgccat ccaagaagat
      481 gcacaagcta ttgcaatgac gtcctatcaa ggtgggcact tagagtattt caaatacatg
      541 tatgacttat tgaaacaaaa aggtgcacct catattaaaa ttgttggtgg aggtggtgga
      601 accatccttc cgtctgaaat taaagaattg gaagaatacg gtatcacccg catttatcac
      661 ccagacgatg gtcgtgcttt gggattgcaa ggtatgatta atgaagtggt tgaattgtgt
      721 gatttcccaa caggtcaaaa cctgacagac gaagtttcaa agttgaaaga taaacacgta
      781 cctgctattg caaaattgat ttctgctgct gaaaatttcc cagaagaatc aaaagcagtt
      841 tttgctcaaa ttcatgaact agcagcgcaa catcaagtgc ctgtacttgg aattacagga
      901 actggtggtg ctggtaagtc ttctttagtg gatgaattgg tgcgccgttt cttaattgat
      961 tttccaacca aacaaattgc agtagtttcc atcgacccgt ctaaaagaaa aacgggtgga
     1021 gctttgttgg gcgatcgtat ccgtatgaat tcgattaaaa acgaacgagt ttacatgcgc
     1081 tcattggcta cacgtcaatc caatttgtcg gtttctaaat atgttcccga agcgttggcg
     1141 gtgttaaaag cagccaatta tgatttaatc atcctagaaa catctgggat tggtcagtcg
     1201 gatacagaaa ttatggattt ctcagatgct tcactctacg taatgacgcc agaatatggg
     1261 gctgctacac agttggaaaa aattgacatg ttggattttg ccgatgtgat tgccttgaat
     1321 aagtttgata aacgtggtgc tttggatgca ttgcgcgatg tgcgtaaaca ataccaacgc
     1381 aaccacaatc tttttgattt gccagttgat accatgcctg tttatggtac aatcgcttct
     1441 caattcaacg acccaggtat gaactctttg tacaaagtgg taattgaaaa agtaaaagaa
     1501 aaaacgggtg cagatttgaa ttcaacgttt caagtaacca aagaaatgtc ggagaaaatt
     1561 tatgtgattc cacccgaccg cacacgttac ttatcagaga tttctgaaaa caaccgttcc
     1621 tacgataaat gggtcaacca acaagttgaa gttgccgata aattgtatgg tttaaacaca
     1681 tccattgaag ctttaaaaca atcaaaacta gatgataaag atcgtttaat caaaggattg
     1741 caagaagctt ttgaattaga aaaattaaat ttcgacccta aaaactgggc aataattgaa
     1801 aattgggcaa ccaaaaaaga taaatacaaa caaccaaaat ttgaatttaa agttcgtgat
     1861 aaggtgttaa cgttggatac acacaccgaa tcattgtcgc acacacaagt gccaaaagta
     1921 gctttaccta aattcaaatc gtggggagat attttacgtt ggaacttgca agaaaatgtt
     1981 ccgggcgagt ttccttatac ttctggattg tttccattta aacgtgaggg agaagaccca
     2041 acacgtatgt ttgctggaga aggaggaccg gagcgtacaa acaaacgttt tcactacgta
     2101 tcattgggaa tgccagcaaa acgcctttca acagcatttg actcggtaac attgtatgga
     2161 aatgaccctg caactcgtcc cgatatttac ggtaaaattg gtaactctgg tgtttctatt
     2221 tgttgtttgg acgatgctaa aaaattgtat tcaggatttg atttaacgca tccactaaca
     2281 tctgtttcta tgaccatcaa cggtccggca cctatgctgt taggattttt catgaatgcg
     2341 gcaatcgacc aaaattgtga aaaatacatt aaagaaaatg gcttagaagc agaagttgaa
     2401 gctaaaattg aagctattta taagaaaaaa ggagtagctc gccctcgtta ccaaggtgaa
     2461 ttgcctgaag gaaacgatgg tttaggtttg atgttactcg gcgttacagg tgatcaagtg
     2521 ttaccaaaag acgtttatga gcaaattaaa gctactactt taaaatctgt tcgtggaacg
     2581 gttcaagcag atattttaaa agaagatcaa gcgcaaaata cgtgtatttt ctcaaccgaa
     2641 tatgcgttgc gtttaatggg tgatgtacaa gaatatttta ttcaaaaagg agtgcgtaac
     2701 ttttattctg tttccatttc gggttaccac attgcagagg caggtgccaa tccaattact
     2761 caattagcat ttacgttggc caatggattt acgtatgtgg aatattattt gtcacgtgga
     2821 atggatatca atgaatttgg tccaaattta tcgtttttct tttccaatgg tatcgaccca
     2881 gaatatgcag ttattgggcg tgtagcgaga agaatttggg caaaagcttt ggatagaaaa
     2941 tacggtgcag atgcccgttc acaaatgttg aaataccaca ttcaaacttc tggtcgttca
     3001 ttacatgcgc aagaaattga tttcaacgat attcgtacca cattgcaagc tttgtatgcg
     3061 atttacgaca actgtaactc gttgcacacc aatgcttatg atgaagctat tacaacacca
     3121 actgaagaat cggttcgtag agcaatggct attcagttga ttattaaccg tgagttggga
     3181 ttggctaaaa acgaaaatcc attgcaaggt tcattcatca ttgaagaatt aacggattta
     3241 gtggaagaag ccgtattgac tgaatttgac cgcattacgg aacgtggagg ggtgcttgga
     3301 gcaatggaaa ccatgtatca acgtggaaaa attcaagaag aatcgttgta ttacgaaatg
     3361 ttgaagcata cgggagagtt tcctatcatt ggggtaaaca cgtttttgag ttccaaaggt
     3421 tcgccaaccg ttgttcctaa agaagttatt cgtgcaacag aggaggaaaa acaataccaa
     3481 attgagatgt tgaacaactt gcaccaagga aataaagccc aaacagaaga aggtttagca
     3541 aaagtgcaag aagcagctat tcaaaataaa aatatctttg aagtattgat ggaaacttgt
     3601 aaaacagcat cgttgggtca gattaccaac gccatgtttg aggtgggtgg tcagtaccgt
     3661 agaaatatgt aatttttaag caacaacaag gcaagaattg caacaaatga aagtaaatac
     3721 aacacttggt tttctttttt tggtaattct tgctacaagt tgtgcttcca tcaaacccac
     3781 tgcgcccgat tttttgaaag aacaacaaca gatgccttca acttcggtga gcaatcaaat
     3841 tgttgtgcct ttagaagtaa acctatccgg tttggtgaaa gaagcagaaa acagtgtgcc
     3901 tgcagtaatg aaaggtggcg attatccgtg cagtggtgtg cgctattctt atgagtttta
     3961 caaagataaa ttccaaatta ctgccgataa acaagtcatt acctctaaac taaacggctc
     4021 ttattggatt aagatgaatt attgtgccgg ttgcacgggc ttgtttggcg atcaaaattg
     4081 catttctcca gttattccgt tttcgtgtgg tgttaatgaa accaaaccat ccgtagaaat
     4141 agttctttca actaaaattg gcatcacaaa agactatgca ctcactgcca ccacaaaaat
     4201 tgacaaagta gagcccatcc atccatgtaa agtaaccgtt tttagattcg atgcaactgg
     4261 cgaagtgatg aaacgcgtaa cagccgttgt agaatccgaa gccaaagaaa tggataaaaa
     4321 attgggagaa attcaattta aaaatgaatc cgaagcattt tggaaacaac tcaaccaaag
     4381 ttttaaaatc cccaatttag gttttttgca tgtgcaacca aaagcaattt ctattgtaaa
     4441 accacgcttt gaaaacaata aattgtattc ggcattgctt ttaaattgca ctacgtttat
     4501 taatcaagag gaaaaaaata gcgcaacaag taaattgccc gaattgcaag ttgtttccag
     4561 cacgcccaaa gatacttttg aagtgtttac cgatattacc ttggattacg actctttatc
     4621 ccagcttatg accactcaat tgggtggtaa aacgctggaa ttgcgcaata aacaatttgt
     4681 ttttaccaag tttgatgtca atcccttgcc cgataatcgc attgtgattg gcgtgcattt
     4741 tgatggttca aaaaatggca ttttatatct aaaaggacaa cctgtttttg acaacaacac
     4801 aaaagttttt agtttaacgg caattgaata cgatattgaa acaaaaagtt cactcattaa
     4861 atttgccgat tggttgttta gcaaaaaaat aaccgatgaa ttgcaaaaag cgagtacggt
     4921 tgattttaaa tccgattttg aaaaattaaa agccgaaatc aatcaaaatt taaaacgaaa
     4981 aattggcgat tatacgctca gcggtaaagt gcacgatgca aaagtgcaca acatctatgt
     5041 ggatttaaac catttgtttt tacgaacagg cgtaactgcc aatttaaaag tgactgatta
     5101 aaatcattgt ttttccacta taaatacctt atttttacag caaattttta gaagatgaaa
     5161 aattatttaa caattattgc tactgcattc ctattcgttg cttgtaacgg aaaacaacaa
     5221 gaagatacta ctgcaccaga agttgtttat tctaaatttg gaacagaaat tacccccgat
     5281 aaagccattt cgaaagcaga aatgctagaa atgtataaaa gcatgaaaga gggtgatacc
     5341 gtaaaggtga aatttcaatc tgaaatttta gatgtttgtc agaaaaaagg atgttggatg
     5401 accatggatt tggatgaaga aaatgaagtg tttgttcagt ttgccgatta tggttttttt
     5461 gttcctaaaa atgccgcaaa tcacaatgga attgtagaag gaaaggcatt tgtgagcatt
     5521 accagtgttg acgatttgaa acattatgcc aaagatgcag gtaagtcgca agaagaaata
     5581 gacgctatca ccgaacctga attttcttac tcttttatgg cggatggtgt tttattggat
     5641 gaaaatctag cttcaaaatc gaaataaaat gaaagcattt tattggctct tttttggttt
     5701 gttgcttgca agttgcaacg aaaaaacgca aaacgttgag gaaaattcgt ccaaacaatc
     5761 accctttgaa atgtacaaaa tttctgaaat ggcagcattg atggaaaaaa tgtacaccaa
     5821 caaccaagca atcaaacaaa aaattgagca aggaaacacc gatttgggcg tttttccgga
     5881 agaatacaaa accatccacg ttgccacctt gaccgatcct tcggatagag atgatttttt
     5941 taatgaacac gccaaaaagt tcatagcagc gcaagagctt gtttttagtg aatcaacccg
     6001 tcaaaaagaa aatttcaaca atatggtaga tgcctgcatt gcgtgtcacc aaaaaaaatg
     6061 tggcggaccc atcgttagaa ttaagaaatt gtacattaaa taagtcccta tgttctttga
     6121 ttttttaagc cgtctgcaag ccaataacaa tcgggagtgg tttgaagcca ataaaaaaca
     6181 atatgaacaa gcaaaatcgg aagcagcaca aatttttgaa cgcatttatg cagaattgat
     6241 tcaattggat gcgtttgaaa aattaaaaat gtatagcatt taccgcgatg tacgcttttc
     6301 acacgataaa acgccctata aaaaccattt cagtgccatt tttatgcgtc gtcaaccgca
     6361 caacagaggg agtttttatg tacatttaga acctaataac tcctttattg gtgcaggatt
     6421 ttgggggccc gaaaaagaag atttattgcg cattcgccaa gcaatagaaa tagaagacga
     6481 tcttgaaaac attctcaaac aaaaagaatt aacaacatct tttggtgcaa tgatgggtga
     6541 ggcagtaaaa tcggctccca aaggttttga taaagagcat cctcgtattc acttaattcg
     6601 tcacaaacaa tttattttca aaaaatcatt tgcagataat tgggcgcttc aaccagattt
     6661 tgcacaacaa gtggctgctg attacaggat tttacaacca ttttttcagt acatgaccga
     6721 tgtgttaacc actgatgcta atggtgaacc cctattttaa ggttgcggtt ttacttcttt
     6781 tttcttcaac gttaatttca cttcgccttg atgaaattta ggaaaatgag ttcctggctc
     6841 aaattgtggt cccatcaagt agttgtatat gcgcacatca agtggaatgg aaacttttat
     6901 tgatttatta ccattgattt tagcactggt tacctttcca tttatatcaa cagaaatggt
     6961 gtaatatacc tcgccaaccg atgattcata catcgtaaaa tcagatggag tagttaattt
     7021 ccgcccatct tttgccaaat cgccaccaac aacttgcgaa aacgatacaa aagaaaatac
     7081 caagctgagt gccactaaaa aagttgtttt cattttttaa attttctcga agatacgaaa
     7141 tccaaataca agcattcctt cgtttcaaaa aaataattaa cattgtaaca aacaatatta
     7201 atgctatgga ttatcctgct tcagaattag tactagacag ccaaaaccga gtgtatcacc
     7261 ttggaatttc tcctgaaaat tgtgcacaca aaataatttt agttggcgat caagaccgag
     7321 ttgccttggt tagttccttt tttgacacca ttgaatttac ccatcaacac cgcgagtttg
     7381 taacccacac aggcacgtat aaaggaaaaa gaattacggt gctttcaaca ggcataggca
     7441 ccgataatat tgacattact gttaatgaat tagatgcatt ggccaatatt aatttaaaaa
     7501 atcgaacaaa aaaatcaaac cacacagcgt tggaattggt gcgtattggt acatgtggaa
     7561 ttttacaacc aaccatacct gtacattcct acattttaag tgaagctgct ttggggttgg
     7621 ataatgtggc tcatttttat aatgttcctt tttcagaaaa tgaaattcaa gaatgcacca
     7681 aaatagaaca tcatttaggt ctccctgaaa aagtaaaacc ctattatatt gaagcagcaa
     7741 aagaattggt cgaaaaattt agttcaccag caaccaagaa cggtattacc ataactagca
     7801 gtggttttta tggaccacaa ggccgacaat taaacctatc aacaaaaaca acccaattaa
     7861 acgagcgttt aaccaattat gcctcatccg atttaaaagt tgtcaatttt gaaatggaaa
     7921 gttcggcact atttgctttg tccaaagcct tgggacataa aagcactacc atttgtttag
     7981 gtattgctaa ccgtccgaaa ttagaatttt ctaaagatta ttctgaacca atgaacgcct
     8041 taattgtata tgttttagag agaatataag taggaaaaac cgcaatttga atagaataaa
     8101 agaaagataa aacagaaaaa catacgtttt agttacgaaa ttgtaccgaa aaataaaaaa
     8161 aagttactgt tcaggtgtag atgtgttatt tttcaaatca ttttattaag tttgcggtta
     8221 aaactaaatt taaaaactta aaaattttat tatgagtggt aaaaaatcag caggaggatt
     8281 ctctgcacta atgggagcaa ttgctattgt aattgcagta atcgcgggta ttttaattta
     8341 caaatttatt tttggtaacc cagcaaactt cgtggataac aatccagaaa acgaaccttt
     8401 acaaggaaat ttcttaggaa ttatctacaa aggaggtttc atcgtaccaa tgttggttgc
     8461 aatcaactta atcgttgtaa tctttacaat tgagcgtttt gttactttag gaaaagcaaa
     8521 aggtaaaaac agcttaaatg tatttgtaga aaaagtgaaa gcagcattag ctggagggaa
     8581 cattgcagaa gcaagagaat tgtgtgaagc acaaaaaggt tctttggcaa atgtagttgt
     8641 tgctggtttg gataaatatg aattgatggc taacgaaaca tcaatgaaca aagaacaaaa
     8701 aattgaaagc gttaaaaaag aattggaaga agcaacttct ttggaaatgc caatgttgac
     8761 taaaaactta tctgttattt caacaacagc ttctatttcg gtattagttg gtttgattgg
     8821 tacggtattg gggatgatca aagcgtttgc ggcgatgtca cacggtgcac cagatacagc
     8881 acagttagca acaggtatct ctgaagcctt gattaacacg gcgttgggta ttacaggttc
     8941 tacagtttct atcatcttct tcaacatgtt ctca
//
LOCUS       DAOQVQ010000047         8722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_8957_length_8722_cov_3.726087, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000047 DAOQVQ010000000
VERSION     DAOQVQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8957_length_8722_cov_3.726087"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKN22_02595"
     CDS             complement(<1..795)
                     /locus_tag="PKN22_02595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNS41622.1"
                     /translation="MDQFFLKLSPSKSALTLTHGDAIGLIGSCFSDEIAKKLKNAGHL
                     VQSNPWGTLFHPTAMAYQLLDCFNNDLDQRIFERNGLYYSWNTSTLIHGTTKESVQQQ
                     LLALKQAFHSFIKNSKILIVTFGSAWGYELKEDQQLVANCHKMPSQLFDKFLSSPYEL
                     VQDWVLVLEKLQELNPTINVVFTVSPVRHIKDGIVENNLSKARLIEAVHQLSYEPNCA
                     YFPSYELVIDVLRDYRFFKEDRVHPSAEAINFLWNHFEKTFFDDTTI"
     gene            complement(1022..2920)
                     /gene="argS"
                     /locus_tag="PKN22_02600"
     CDS             complement(1022..2920)
                     /gene="argS"
                     /locus_tag="PKN22_02600"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNS41623.1"
                     /translation="MEHLIKSKIIENAQTLFNAPINEQLVQLQKTRKEVEGDLTLVIF
                     PFVKMMRCSPQEAGQIIGDFLVKEIPAISKYEVVNGYLNLVISTDFWVNELKNIHADD
                     NFGLQTPNSKPTIMVEYSSPNTNKPLHLGHLRNIFLGFSVAEILKANGHKVVKTQIIN
                     DRGIHICKSMLAWQKFSPINAQGERETPQNTGIKGDKLVGKYYVEFDKHFNAEAKAII
                     ALWEQGNFENYSTAIKDEFIRLTEAKNGKDEKAIKAIDDKIKDLAKNQTSILKEAKEM
                     LVKWEAKDAEVYDLWSTMNQWVYDGFEKTYTTMNVDFDELYYESNTYLLGKDIVEEGL
                     KKGVFFKKDDGSVWIDLTADGLDEKLVLRSDSTAVYMTQDMGTAVERYNSHPDLSGIV
                     YTVGNEQDYHFKVLFLILDKLGYSWAKNCFHLSYGMVDLPEGKMKSREGTVVDADDLM
                     NEVVESATEMTKERGHIEGLSENDLSNLCQMIGLGGLKYFLLKVDPKKRMKFNPAESV
                     ELNGNTAPFIQYAHARIQSLLAKSTGELNTINPSEVNDVEKEIIKQLTLFPSIIEEAG
                     RDYAPSVVANYTYELVKLYNQFYQSVTILNESDEQLKQFRLILSKNVAKTIKTAMKLM
                     GIDVPNRM"
     gene            complement(2921..3874)
                     /locus_tag="PKN22_02605"
     CDS             complement(2921..3874)
                     /locus_tag="PKN22_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNS41624.1"
                     /translation="MKNIKFIINRSEITAGTRGASLGPDALKTVAWKQEKELFGNYPS
                     CEIINENHFLNHPPKHQFAKRIEGLISIFNDVCKQLQDTYKQQQFPIILSGDHGSAGG
                     TIAGIKAAFPNKRLGVVWIDAHGDLHSPYTTPSGNMHGMPLNTALDDNNTQCQRNALP
                     AETESLWNELKSIGFKGQKVAPEDLIFIGVRDVEEEEIALMQRLNIRNFTVQEVQQKG
                     AATVIDEIEQQLNNCDIIYVSFDVDSMDPEETSHGTGTPVPNGLSVKDTQTILTKLAA
                     NPKLIGFEVVEINPCLDEKTNKMAETAFGLLENVITTIENK"
     gene            complement(4039..7815)
                     /locus_tag="PKN22_02610"
     CDS             complement(4039..7815)
                     /locus_tag="PKN22_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNS41625.1"
                     /translation="MCFVQDDNSGLWIGTQDGLNRFDGKKFDVYTTDNTPGLKGNYIH
                     DLINDAKGNTWIATGNGLTFRDKKTEVFTTYNNGGDLFSIESIAFDKDKTTIWIATSR
                     KGVWTFDTKTKTFSPKTSIFVTKLCRKIFISQEGYVIVASDDNELEILNPQTSQLKKI
                     SFEKRFKSLTINTIYQKNKNEVILGSNQGLFSCNLSTFTTSVLFQNINKEFGYLNISG
                     VINTEDNHWYISTTNNGLITVLPNGDIINSTEDIYQKNTLIFNNINTIFEDKNKTIWL
                     GTSRGISGFDPISQGFLGISVSGDIQRGLTSPNVWCIKEDTKSNMLYIGTDEGISRFD
                     TKKRTFTHFNRNNKNNIHISNTEKAVLDIQIINEHSVLAACVDGIYELHIKNDGSHTY
                     TPISFVGKNIKSQFTTAYKIVEYKPNSYFFATRNGALLVDYNTKTTQEFVNDAKNPTA
                     TILPGICRVVYKNSKNQLLFATSSGGLSVLDDSNPKDLKIKPFNYNNTIFKNIASYIS
                     YIVETSKNEYYFGTAGDGIIYWNTKTQKVKTYNKKNGLPNNVVYAIIPDNQQNLWIST
                     NKGLSRFELATKTAFNFTELHGLLSNEFNMGAGLKSASGSIYFGGIAGLVFFNPNSLS
                     NYNMVLNVSLTKFKIDNEWITPSSPKSPLLASISYTNSIELSYKQRSFAISFQTDNIS
                     ATELINYKYHLESRGDEGKDIEIGNSNEIQFNSLSPGHYTLKIYARIGYGEWVSKPKT
                     ITIIIQPPFWNTWWFWVICSIALILIIRFSITKRIQAERRQQVRLEMKIAERTAEIRE
                     QHKKIEQQNNELEKQRQQLEIEKEKSDRLLRNVIPDSMADELLTKGFASARAFKVVSV
                     MFTDFVGFTKITDSMSPTELVKKLDVFFRKFDEIIFDNNLERIKTIGDAYMAAGGVPV
                     RNTTNPIDICLAGLQIQHYIKELEKETIDAGEMPWKLRIGINTGEVTAGVIGTKRLAY
                     DVWGATVNYAQRMEMFGEAGKVSITRNTYLHIKPYFECTYKGLISAKGEEKVQMYTLD
                     RIKPELSVDGKGIYPNERFHKIVNLHHYSSINYYKAERFIMKKLENELSSKLHYHSIE
                     HTLDVVKAVERIALSENVTDEGLFLLKTAASYHDAGFVEQYDKNEPVGVRMANEILPK
                     YGYTPEHIEIIKKLIFVTSVPHQPTNLLEEIICDADLDYLGRDDFHQIADKLRLELRE
                     HGKINSDRAWDEIQVRFLEQHRYFTQTSLKTRMAKKLQNLEEIKERLKRNEYKD"
     gene            complement(8071..8547)
                     /locus_tag="PKN22_02615"
     CDS             complement(8071..8547)
                     /locus_tag="PKN22_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS41626.1"
                     /translation="MVNNVYIRRVEISDLSTLLLWENDPNNWEVSEVNQPFSEEEIAD
                     FIVNQQASINALDQIRWMICLKSTHEAIGTIDLFDIQTNQAGVGILIQSTHHRNKGYA
                     TKALELLKKLAKEDLKLDHLFCNIQHFNLTSIHLFEKCQFKQVEKFEKHAKYSVNL"
BASE COUNT         2558 a         1530 c         1529 g         3105 t
ORIGIN      
        1 aattgttgtg tcatcaaaaa acgttttttc aaaatggttc cacaaaaaat tgatggcttc
       61 ggcagatgga tgaactctat cttctttgaa aaaacggtaa tcgcgcaaaa cgtctataac
      121 caattcgtag gatgggaaat aggcacaatt gggttcgtat gaaagttgat gaactgcctc
      181 aattaatcgc gcttttgata aattattttc aactatccca tctttgatat gacgcacagg
      241 actaaccgta aaaacaacgt tgatagttgg atttaattct tgcaattttt ccaaaacaag
      301 cacccaatct tgcaccaatt cataaggact tgaaagaaat ttatcaaaca attgagatgg
      361 cattttgtgg caattggcta caagttgttg gtcttctttt aactcatatc cccaagcaga
      421 accaaaggtt acgataagta tttttgaatt ttttataaac gaatgaaacg cttgtttgag
      481 tgcaagcaat tgttgctgaa cactttcttt tgttgttcca tgaataagtg tgctagtgtt
      541 ccacgaataa tacaaaccgt ttctttcaaa aattcgttga tcaaggtcgt tgttaaaaca
      601 atccaacaat tgataagcca ttgctgtagg atgaaataaa gttccccacg gattgctttg
      661 caccaaatga ccagcatttt tgagtttttt tgctatttca tccgaaaagc aactgccaat
      721 taaaccaatt gcatcgccgt gcgtaagcgt taatgctgat tttgaaggag agagtttaag
      781 aaaaaattga tccatagaaa ttgctacaac ttataaaaaa caaaaagagg aagtaaaacc
      841 cttgtaatta cttcctcttt tcgctaaacc atgctaaaac tataaaaccc aaaaactaca
      901 actatgaatt gcgttattta gataggacaa atgtacgcca aaagttttag ttagaaaaat
      961 tttttcaata aaaaaatgtt aaaaaatata aattaacagc aaaatgctgt atgtgaatta
     1021 tttacattct gtttggaacg tcaattccca ttaacttcat tgctgttttt atcgttttag
     1081 ctacgttttt actcaaaatc aaacggaatt gtttcaattg ttcatcggat tcattgagaa
     1141 tagttaccga ctgataaaat tgattgtaca atttcactaa ttcgtaagta taattggcca
     1201 caactgaagg tgcataatct cttccagcct cttcaatgat acttgggaac aaggttaatt
     1261 gtttgatgat ttctttttcc acatcgttca cttccgaagg attgatggta ttgagttcac
     1321 cagttgattt tgccaacaac gattgaatac gagcgtgtgc atattgaata aatggagctg
     1381 tgtttccgtt caattcaacc gattcagcag gattaaattt catgcgtttt ttaggatcta
     1441 ctttcagcaa gaaatatttt aatccgccta aaccaatcat ttggcacaag tttgataaat
     1501 cgttttcgga taaaccttca atgtgtccac gttctttggt catttctgtt gcactttcca
     1561 ccacttcgtt cattaaatcg tcggcatcaa ctacagttcc ctcgcgtgat ttcattttcc
     1621 cttcaggtaa atcaaccatt ccataagata ggtggaagca attttttgcc cacgaataac
     1681 ctaatttatc aagaattaag aataaaactt tgaaatggta atcttgttcg tttccaacgg
     1741 tgtaaacaat tcccgacaaa tcagggtgac tgttgtaacg ctctaccgct gttcccatgt
     1801 cttgcgtcat ataaactgcc gtactgtctg aacgcaatac caatttttca tccaaaccat
     1861 cggctgtaag gtcaatccaa acagaaccgt cgtctttttt gaaaaacacg ccttttttta
     1921 atccttcttc aacaatgtct ttaccaagca aataagtgtt tgattcataa tacaattcat
     1981 caaaatctac attcatggta gtataggttt tttcaaatcc atcatacacc cattggttca
     2041 ttgttgacca taaatcgtac acttcagcat cttttgcttc ccattttacc aacatttctt
     2101 tggcttcttt gagaatagac gtttggtttt ttgctaaatc tttaatttta tcgtcaattg
     2161 ctttaattgc cttttcgtct ttaccgtttt tagcttcggt taaacgtata aattcatctt
     2221 taatcgccgt tgaataattc tcaaaatttc cttgttccca caaagcaata atggctttcg
     2281 cttcggcgtt aaagtgtttg tcaaattcaa cgtagtattt acctaccaat ttatcgcctt
     2341 tgatgcctgt gttttgagga gtttcacgtt cgccttgcgc gttgataggt gaaaattttt
     2401 gccatgccaa catgctttta caaatgtgaa ttcctctatc gttgatgatt tgagttttca
     2461 caactttgtg accatttgct tttaaaattt cggcaacaga aaatcctaaa aagatatttc
     2521 taagatgacc taaatgcaat ggtttgttgg tgttaggcga tgaatattct accataatgg
     2581 taggttttga attgggagtt tgcaatccaa aattatcatc ggcatgaatg ttttttaatt
     2641 cattcaccca aaaatcggtg ctaataacca agtttaaata gccgttaacc acttcgtatt
     2701 tgctgatagc aggaatttct ttaactagaa aatcaccaat tatttgtcca gcctcttgcg
     2761 gagaacagcg catcatctta acaaaaggga agataaccaa tgtcaagtcg ccttcaactt
     2821 ctttgcgtgt tttttgaagt tgaaccaact gttcattgat tggagcatta aacaatgttt
     2881 gcgcattttc aataatcttc gatttgataa gatgttccat ttatttattt tctattgttg
     2941 ttattacatt ttctaaaagc ccaaacgcag tttcggccat tttattagtt ttttcatcca
     3001 aacatggatt aatttcaacc acctcaaaac caattagttt aggattagca gcgagtttgg
     3061 taagaatagt ttgtgtgtct tttaccgaca aaccatttgg cacaggtgtt ccagttccgt
     3121 ggctggtttc ttctggatcc attgagtcaa cgtcaaaaga aacgtaaatg atgtcgcaat
     3181 tattcaattg ttgttcaatt tcgtcgatta ctgttgcagc gcctttttgt tgcacttctt
     3241 gaacggtaaa attacgaata tttaagcgtt gcatcaaagc aatttcttct tcttccacat
     3301 cgcgcacacc gatgaaaatg aggtcttctg gagcaacttt ttgtccttta aacccaattg
     3361 atttcaattc gttccataaa ctttctgttt cagcagggag tgcattgcgt tgacattgtg
     3421 tattgttgtc atccaacgct gtattgagtg gcattccgtg catatttcca gatggagtag
     3481 tgtagggcga gtgcaaatca ccatgtgcat ctatccaaac tacacccaat cgtttatttg
     3541 ggaaagcagc tttaattcca gcaattgtgc caccagccga tccatggtcg ccagataaaa
     3601 taataggaaa ttgctgttgt ttgtatgtat cttgcaattg tttgcaaaca tcgttaaaaa
     3661 ttgaaatcag cccttcaatt cgtttcgcaa attgatgttt tggaggatga tttaagaagt
     3721 gattttcatt aataatttcg caagatggat agttgccaaa taattctttt tcttgtttcc
     3781 aagcaactgt tttaagggca tctggtccca atgaagctcc tcttgtgcca gcagtaattt
     3841 cagagcggtt gattataaat ttaatatttt tcataacgag agaataacca atcattcagc
     3901 ttaattaatt tgtttttttt gaaagcctag aaagcaatga attactttcg agattagtta
     3961 ttttgtgtgc caaagataag ttgaaaatat gaaataatgg tggtgttgaa atgctaattt
     4021 gacctttttt tccacaaatt agtctttgta ttcgtttctt tttaatcgtt ctttgatttc
     4081 ttctaagttt tgcagttttt ttgccattct agttttaagc gatgtttgtg taaaataacg
     4141 gtgttgctcc aaaaatctaa cttgaatttc atcccaagct ctatcggagt taattttgcc
     4201 atgttctctt aattccaaac gtaatttatc ggcaatttgg tgaaaatcat ctcttcccaa
     4261 ataatctaaa tcggcatcgc agataatttc ttctaataaa ttagtaggtt ggtggggcac
     4321 actggtaaca aaaattagtt ttttgataat ttcaatgtgt tctggcgtgt agccatattt
     4381 aggtaggatt tcattcgcca ttctaacacc aacaggctcg tttttatcgt attgttccac
     4441 aaaaccagcg tcgtgatagg aagcagctgt ttttaataaa aacaatcctt cgtcggtaac
     4501 gttttcgctc aaggcaattc gttcaactgc tttgacaaca tcgagcgtat gttctatgct
     4561 atggtagtgc aatttgctgg agagttcatt ttccaacttt ttcatgataa aacgctccgc
     4621 cttgtagtaa ttgatagaac tgtaatggtg aagatttaca attttatgaa aacgttcgtt
     4681 tggataaatg cctttaccat caaccgataa ttctggtttg atacgatcga gggtgtacat
     4741 ttgcactttt tcctcacctt tagcagaaat taatcctttg taggtgcatt caaaataagg
     4801 tttaatgtgc aagtaggtat tgcgtgtaat ggatactttt ccggcttcgc caaacatttc
     4861 catgcgttgt gcgtagttaa cggttgcacc ccatacatcg taggctaaac gttttgtacc
     4921 gataacccca gcggtaactt ctcccgtgtt gattccaatt cttaatttcc acggcatttc
     4981 tccagcgtca atggtttctt tttctaattc tttgatgtag tgttgaattt gcagacctgc
     5041 taaacaaatg tctattgggt tggttgtatt tcttacagga acgccacctg ccgccatgta
     5101 ggcatcacca atagttttga tacgttccaa attgttgtca aaaataattt cgtcgaattt
     5161 tctaaaaaag acatccaatt tttttaccaa ttcggttggc gacatagagt cggtgatttt
     5221 ggtaaatcca acaaaatcgg tgaacataac agaaaccact ttaaatgcac gtgcacttgc
     5281 aaaacctttg gtgagtaact catctgccat gctgtcgggg atgacattgc gcaataaacg
     5341 gtctgatttt tctttttcaa tttctagttg ctgacgttgt ttttcaagtt cgttgttttg
     5401 ctgttcaatt tttttgtgtt gttctctaat ttcggctgtt ctttcggcaa ttttcatttc
     5461 caaacgcact tgttggcggc gttctgcttg aattcttttg gtaattgaaa aacgaataat
     5521 aagaataagt gcaatactgc aaatcaccca gaaccaccaa gtattccaaa atggaggttg
     5581 tatgatgatg gtaattgttt ttggttttga tacccattca ccatagccaa ttcttgcata
     5641 aattttaagg gtgtaatgtc ccggagaaag tgagttgaat tgaatttcat tgctgttgcc
     5701 aatttctatg tcttttcctt cgtcgcctct actttcaaga tggtatttgt agttgattaa
     5761 ttctgttgca gaaatattat ctgtttgaaa actaatggcg aaacttcgtt gtttgtagga
     5821 taattcaatt gagttagtat aggaaataga agccaacaaa ggcgattttg gggatgatgg
     5881 tgttatccac tcattgtcaa ttttgaattt tgttaatgag acatttaaaa ccatgttgta
     5941 gttgctcaac gaattggggt tgaaaaacac caagcctgca attccgccaa aatagataga
     6001 acccgatgcc gattttaaac cagctcccat gttaaattca ttgctcaaca atccgtgcaa
     6061 ttctgtaaag ttaaaagctg tttttgttgc taactcaaaa cgactaagcc ctttattggt
     6121 gctgatccat aaattttgtt gattatcagg aatgatggcg tacacaacat tgttgggaag
     6181 tccgtttttt ttattataag ttttaacttt ttgagttttt gtattccaat aaattattcc
     6241 gtcgccggca gtaccaaaat aatattcgtt tttactggtt tcaacaatat aggaaatgta
     6301 ggaagctatg tttttaaaaa tggtgttatt ataattaaac ggcttgattt taaggtcttt
     6361 tggattgctg tcatctaaaa cagataaacc tcctgatgaa gtagcaaata acaattgatt
     6421 tttagaattt ttataaacaa ctctacaaat tcctggaaga atagttgcag ttgggttttt
     6481 ggcatcgttt acaaattctt gggttgtttt tgtgttgtaa tctactaata gtgcgccgtt
     6541 tcttgttgca aaaaaatacg aattgggctt gtattctaca attttataag cggtagtgaa
     6601 ttgactttta atgtttttac caacaaatga aataggtgta taagtatgag aaccatcatt
     6661 tttaatgtgc agttcataaa ttccgtccac acaagccgca agcacactat gttcgttaat
     6721 gatttgaata tctaaaaccg ctttttctgt attggagatg tgaatgttgt ttttattgtt
     6781 tcggttgaaa tgagtaaagg ttcttttttt agtgtcaaaa cgtgaaattc cttcatcggt
     6841 gccaatgtaa agcatgttgg atttagtgtc ttctttaatg caccaaacat taggtgacgt
     6901 taaaccacgt tgtatatccc cactaaccga aatgcctaaa aatccttgac ttatggggtc
     6961 gaacccagaa atccctcttg aagtgccaag ccaaatagtt ttgtttttat cttcaaaaat
     7021 ggtgttgatg ttattgaata tcagggtgtt tttttggtaa atatcttctg ttgaattaat
     7081 aatatcgcca ttgggtaaaa cggtaatcaa tccattgttg gttgttgaaa tgtaccaatg
     7141 gttatcctct gtgttgatta caccacttat gttgagatag ccaaattctt tgttgatgtt
     7201 ttgaaataaa acagaagtgg taaaagttga taaattgcaa gaaaacaatc cttgattgct
     7261 acctaaaata acttcatttt tgtttttttg atagatggtg tttatcgtta aacttttaaa
     7321 tcgtttttca aagcttattt tttttagttg actggtttgt gggtttaaaa tctctaattc
     7381 attatcatcg gatgctacaa ttacgtatcc ttcttgtgag atgaaaattt ttcggcagag
     7441 tttagtaaca aaaattgatg ttttaggtga aaaagttttg gtttttgtgt caaaggtcca
     7501 cacgcctttg cgcgaagttg ctatccatat tgttgtcttg tctttgtcaa aagcaatgga
     7561 ttcaatacta aacaaatcac cgccattgtt gtaggttgta aaaacttctg ttttcttatc
     7621 tctaaaagtt aaaccatttc cagtagcaat ccatgtattt ccttttgcat cattaattag
     7681 gtcatgaatg taattccctt taagacctgg tgtattgtcg gtggtgtaaa catcaaattt
     7741 tttaccatca aaacgattga gtccatcttg ggttcctatc cacaaaccag agttatcatc
     7801 ttgtacaaag cacaaggccg aactttgtga taaaccttca ctaatggtga aatttttgaa
     7861 attgtaaata ttttgagaaa ataaaaattg acaactccaa caaaagatga taaggagtag
     7921 taattgatat tttattcgtg tgttagccat ttattttttt aaacgggttg tatcttctgt
     7981 tgcgatttat taatacaaac tatcaaatat aacaagaatc tgcttgtaac attcttcaat
     8041 cttaaaaatt gttaagatat tttaaaatta tcacaaattt acgctgtatt tggcgtgttt
     8101 ttcaaacttt tctacttgct tgaattggca tttttcaaag agatgaatgc tagttaaatt
     8161 gaaatgttgg atgttgcaaa acaaatggtc taatttcaaa tcttctttgg ctagtttttt
     8221 tagcaattct agagcttttg ttgcatatcc tttattacga tggtgtgttg attgaattaa
     8281 aattccaaca cccgcttgat tggtttgaat gtcaaacaaa tcaatggtgc caattgcttc
     8341 gtgcgtgctt tttaagcaaa tcatccaacg aatttgatct aaagcattga tggaagcttg
     8401 ttggttaaca ataaaatcgg caatttcttc ttctgaaaaa ggttggttaa cttcgcttac
     8461 ttcccaatta ttggggtcgt tttcccaaag taggagggta gataaatccg aaatttctac
     8521 tcttcgtata taaacgttgt tgaccatttt gaattatccg ttaaatcgaa atttcaccgt
     8581 tgaacacaaa ggtgccagga ccaatcaacc aaatatctgt aaaacgagca ccatcgtagt
     8641 gaaactgaac agccaaattg cctcccattg cttgaatgtg aatacgattt tgccctagaa
     8701 gttgattttt atgtagatcg ga
//
LOCUS       DAOQVQ010000048         8582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9139_length_8582_cov_3.777178, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000048 DAOQVQ010000000
VERSION     DAOQVQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9139_length_8582_cov_3.777178"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKN22_02620"
     CDS             complement(<1..571)
                     /locus_tag="PKN22_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41627.1"
                     /translation="MIAYTIFIFLLLIVLLRYVPQFRLLGANPWVMPIGFTIKFAVGL
                     FFIYVYSYHYGVGELTADASAFLHDSKELNKVFYTSPLDYFRFMLGIENADMVCNYLA
                     DTEQWDYYGIKWFNDSRNILRLHSIIHFFSFGYQSIHLLIISLISILGLRLMVVATSP
                     YFKIPTILAFFALLLMPSALFWSSGILKEP"
     gene            complement(613..1203)
                     /locus_tag="PKN22_02625"
     CDS             complement(613..1203)
                     /locus_tag="PKN22_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNS41628.1"
                     /translation="MLVEIDDKLISTQLFDRKFVCDLNACKGACCVEGDAGAPLTYEE
                     VDKIEEDLDVILPYMRPEGIEAVKKTGVYYLDEDGEPVTTLVNGAECAFVYFDEHGIT
                     KCAIEKAFLEGKTTFKKPISCHLYPIRVRKFEQYTALNYDEWDICDPACKLGESLNVP
                     VYKFLKEPIVREWGEAFYNELDDVAEQWKNEQEENR"
     gene            complement(1271..2809)
                     /locus_tag="PKN22_02630"
     CDS             complement(1271..2809)
                     /locus_tag="PKN22_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNS41629.1"
                     /translation="MLVKTYSSSVIGIDATTITIEVNLGQGINFFLVGLPDSSVKESH
                     LRIKAAFANNNYKFPQREVTINMAPADIRKEGSVFDLPIAIGVLAVSEQIQSEQLETF
                     QMVGELSLDGNLQPVKGILPMVLQAKKEGFKSVILPYENAQEAAVVDGIDVYGMQSLK
                     EVVDFLNGESTVQPVKIDIEQTFTTLLDDFEVDFKDVKGQQKIKRAFEIAASGGHNVV
                     LIGPPGAGKSMLAKRLSTILPPMTLDEALETTKIHSVAGKIKSSHGLITQRPFRKPHH
                     SISDTALVGGGTYPQPGEFSLAHNGVLFLDELPEFKRSALEVMRQPLEDRVVTISRAK
                     YTIDFPAGVMLIGAMNPCPCGYFNHPEKECSCSQGAVRNYLNKISGPLLDRIDLHIEV
                     TPVSFEELAAEQEGECSKSIRERVINARLIQQNRYTAAKGIYCNAQMGSKEIKAYCQL
                     APESKAILKNAMDRLGLSARAFDRILKVARTIADLDKSEQIATQHIAEAIQFRTLDRM
                     NWGG"
     gene            complement(2888..3232)
                     /locus_tag="PKN22_02635"
     CDS             complement(2888..3232)
                     /locus_tag="PKN22_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNS41630.1"
                     /translation="MAKNKKERINVVYSTNPNFGYEFEEEEEVETLPKNQQKLYVSID
                     KKSRGGKEVTLIEGFVGTEDDLKELGKTLKTKCGVGGSVKDGEILIQGNFRDKVISLL
                     EAEGYQTKRKGG"
     gene            complement(3237..4028)
                     /locus_tag="PKN22_02640"
     CDS             complement(3237..4028)
                     /locus_tag="PKN22_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNS41631.1"
                     /translation="MRFSWLLLFCLFFGCDKQQFSSVEVVGHAGNGLNIQNSIYQENT
                     LQAINLALGLNGISGVEIDVQLSLDQQLWLYHDEKLEEATTASGCIASKNSDELATIR
                     YKSANKEKLTRLDELPFENYTGFTFYLDIRHFNACENQILNQAHFIAALQPIIDGNSN
                     CNFVLITNYPNWLAGFKQKGWKTALNVFNLDELKTINWQNNPFDAVVLRNSNSTTDDV
                     NWIKSQNKNVFLFDIRAPKTIRQAMKKQPDVVIVDDVKAALIEKT"
     gene            complement(4018..4518)
                     /locus_tag="PKN22_02645"
     CDS             complement(4018..4518)
                     /locus_tag="PKN22_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41632.1"
                     /translation="MHKAICCCLFIFSLSFASWGQQRVSTSLSLTKVDFFHGFEYGQQ
                     HQAFEWNVGFEYGINRSIFQKRFFPKLKTNGFYNLIHKPKINLGLGMQYTYSLYRFGT
                     TTKDIVQFHELMGGLRWNVGNQWRFGQTLMVGGLWENDFHTLVNKRQTYGTFGYIIQI
                     DVSYAF"
     gene            4779..6323
                     /locus_tag="PKN22_02650"
     CDS             4779..6323
                     /locus_tag="PKN22_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNS41633.1"
                     /translation="MKHILTLVIAVAATFSFGQKLRFKIADQKDTTIFLVKYNGAKLY
                     YADTAEMKGGVVEFNGAKQKPGMLGLLFPGQQLLEFIYNNEEVNIEAKGPKYAETMVV
                     KKSEENKVFNEYVKFITGKRMDAQKIADEMNKLDKKSAEYKEKQTKIDAISKEVFNFQ
                     NNLATVHSNKLIGKMVKMTIDIDIPEPPVDSKGNIIDSNFRYHYFRDHFFDNVDFSVD
                     GLVNTPIFGNKIEYYFSNKMLIQHWDTILKYSYNLIDKLNPKSKAFEYIVSYVTSTYG
                     KSNQMGMDKVYVMMADKYYCSKNAEGKSPAYWMTEDKLKELCEKIDVQKHLVMGVKAP
                     NIILPDTLDKPWDQLNWVNLHKVDAEYTVLYFWDPECGHCKKVTPKLATLYDKKLKDR
                     NIEVVAIGKATGEEYKKWKDYIKKNNLNFINIAVTEAILKVGKEDARKLIPRFTTLES
                     MNVSQTYDIFSTPRVFVLDKDKNFIAKQISISQLEEMLDRLQGKADLPKLFPPDKEED
                     EHMQSK"
     gene            6433..>8582
                     /locus_tag="PKN22_02655"
     CDS             6433..>8582
                     /locus_tag="PKN22_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41634.1"
                     /translation="MRKLLLLFIAMPAFIFGQGFNDFESVTLPTAYGDGSFTNDGVTY
                     TYGRSRDQDTYPIEDNGLLMQRASDSYLEWTVTNGIGQLDFEYRKAYTGNNVRQLELL
                     VNGTQVATSPTFGGASGEDATIYTFSHAVNQAGIVTIRIKNVGNTATSRQTVIDNIAW
                     TDYTAPAGCDITASGLANVLCNDNNTGADATDDFVTFTLNPTGNDLGTGGYNVSVSAG
                     TITPTTAAYGQATTFTLQNGSAGAGDVTLTITDVNDGACSFTESITDPGTCSSTTPVL
                     NAAPASLTGFNHIVGTPSSSQTITVDGIALQGDVTVAAPTNFEVSLDNTIYTASVLLT
                     PTAGTLASTTVYVRANAAVYGTLSGNLAVSSLNATTVNIALSGFADDYIYRTIDEING
                     YDATTGIADSLNKLVKITGVVQCMDYRAGSGYNMVIIDGTEKGMYIFSTSDKNGYTDP
                     VAGDSIQVKGKVDQFNGLTQVRVDEIQLLKSGSATTSPTVVTMLDESTENQVVRLENL
                     TFVTPTATFPSGSANMDVTDGTNTFTIRVLASMNLAGTATPQGPFNVSGIGSQFDSSN
                     PHLAGYQLLPCGADAFEEICTGANLPDVSVNQAANTLTANASTGVTYQWINCATNAAI
                     AGATNQSFTATTPGSYAVVVSNATCSDTSTCFTVSDASVQSIALENAVKAYPNPFENE
                     LTLTASQTVQFTITDMNGKVIVATSEMKDKVTIST"
BASE COUNT         2797 a         1585 c         1525 g         2675 t
ORIGIN      
        1 ggggttcttt taaaattcca gacgaccaaa acaatgcgct tggcatcaat aaaagcgcaa
       61 aaaacgcgag aattgtgggt attttaaaat aaggcgaagt ggctaccacc attaagcgca
      121 agcctaaaat gctaatcaaa gaaatgatta ataaatgtat ggattgatag ccgaaggaga
      181 aaaaatgaat aatggaatgc aaccgcaaga tgtttcttga atcgttaaac catttgatgc
      241 cataataatc ccactgttcg gtgtctgcta aataattaca caccatatcc gcgttttcaa
      301 ttcccagcat aaaacggaaa taatccaacg gagaggtgta aaaaactttg tttagttcct
      361 tactgtcatg caaaaaagca ctggcatcag cagttagttc tccaacacca tagtggtaag
      421 aatatacgta aataaaaaac aaacctacag caaacttaat ggtaaaacct attggcatca
      481 cccatggatt ggcgccaaga agcctaaatt gaggcacata cctcaaaagc acaatcagta
      541 aaaggaaaat gaaaatagtg taggctatca tctacattta agttttagca aatatacaat
      601 ttgttcaaaa acttaacgat tttcttcttg ttcatttttc cactgttctg caacatcgtc
      661 caattcattg taaaaagcct cgccccattc acgaactatt ggctctttta agaatttata
      721 aacgggaaca tttaaacttt ctcctagttt acaagcaggg tcgcaaatat cccattcatc
      781 gtaattcaaa gcggtgtatt gctcaaactt acgcacacga atcgggtaga gatggcatga
      841 aataggtttt ttaaacgttg tttttccttc aagaaacgct ttttcaatag cacattttgt
      901 tattccatgt tcgtcaaaat aaacaaaagc acattcggct ccgtttacaa gcgtagttac
      961 aggttctccg tcttcatcca aatagtaaac gcccgttttt ttcacggctt caatgccttc
     1021 tggacgcatg tagggcaaaa tcacgtctaa atcttcttca attttgtcca cttcttcata
     1081 cgtcagtggc gcaccagcat ccccctcaac acaacacgca cctttgcagg cattcaagtc
     1141 acacacaaac ttgcgatcaa acaattgtgt agaaatcaac ttatcatcta tttcgactaa
     1201 catcgctttt tttttgcaaa tttcattaaa atatatggaa atgtaaaaga aactcctaat
     1261 tacttgcaaa ttatccgccc caattcattc tatccaatgt tctaaattgg attgcttctg
     1321 caatgtgttg agtagcaatc tgctccgatt tatccaaatc tgcaatggtt ctggcaactt
     1381 ttaaaattcg atcaaatgct cgcgcagaaa gtcccaatct gtccatggca tttttcaaaa
     1441 ttgctttgct ttcgggtgct aattgacaat acgcctttat ttctttgctc cccatttgtg
     1501 cattgcaata aattcctttt gctgctgtat atctgttttg ttgaattaaa cgtgcgttga
     1561 taacacgttc acggatgctt ttactacatt caccctcttg ctctgctgcc aattcttcaa
     1621 atgaaaccgg tgtaacttca atgtgcaagt caattctatc cagcaaggga ccagaaattt
     1681 tgttcaaata attgcgcact gccccttgac tgcacgaaca ttctttttct ggatggttga
     1741 aataaccaca agggcacgga ttcatagcgc caatgagcat tactccagct ggaaaatcaa
     1801 tggtgtattt ggctcgtgaa atggttacca cacggtcttc taatggttgg cgcataactt
     1861 ccaatgcaga acgtttaaat tcgggcaatt cgtccaaaaa taaaacgccg ttgtgtgcca
     1921 acgaaaattc tcccggttgc ggataagtgc ctccaccaac caatgctgtg tcggaaatgg
     1981 aatgatgagg tttacgaaac ggacgctgag tgattaatcc gtgcgaactt tttatttttc
     2041 ctgctacact gtgaattttt gtggtttcca aagcctcgtc taaagtcatt ggcggtaaaa
     2101 tggttgataa acgtttggct aacatggatt ttccagctcc tggtggacca atcaacacaa
     2161 cgttgtgccc gcccgatgca gcaatttcaa atgcccgttt aattttttgt tgccctttaa
     2221 cgtctttaaa atcaacttca aaatcatcta aaagtgttgt aaaagtttgt tcgatgtcaa
     2281 tttttacagg ttgaacagtc gattcgccgt tcaaaaaatc aaccacttct ttgagcgatt
     2341 gcataccata aacatcaatt ccatccacaa cagcagcttc ttgcgcattt tcataaggaa
     2401 gaattactga cttaaagcct tcttttttgg cttgcaacac catgggcaaa attcctttaa
     2461 cgggttgcaa attaccgtcc aacgataatt cacccaccat ctgaaacgtt tccaattgct
     2521 cggattgaat ttgctctgat acagccaaca ccccaatagc aatgggcaag tcaaacacag
     2581 aaccttcttt tcgaatgtcg gctggtgcca tgttgatggt aacttcgcgc tgtggaaatt
     2641 tgtaattgtt gttggcaaat gctgctttga tgcgcaaatg actttctttg accgaagaat
     2701 cgggcaatcc gacaagaaaa aaattgattc cttgtcctaa gtttacttca attgtaatgg
     2761 tagttgcgtc aattccaata acggaactac tataggtttt tacaagcata aaaggtatgt
     2821 tttaaggtta aactttacct aagttaacga aaaaaatgat ttaaccccaa acaattttaa
     2881 aaaaaaatta ccctcctttg cgttttgttt gatatccttc ggcttctaaa agtgaaatga
     2941 ctttatctct gaaatttcct tgaataagta tttctccatc tttaacactg cctccaacgc
     3001 cgcattttgt tttcaatgtt ttacccaatt cttttaaatc atcttctgtt ccaacaaatc
     3061 cttcgattag cgtaacttct ttgcctccac gcgatttttt atcaatggaa acatacaatt
     3121 tttgctggtt tttgggcaat gtttccactt cttcttcctc ttcaaactca tatccaaaat
     3181 taggattggt agaatacacc acattgatac gttctttttt attctttgcc atcgtcttag
     3241 gttttttcta ttaatgccgc cttcacatcg tcaacaataa ccacatcggg ttgttttttc
     3301 attgcctgac gaatggtttt gggtgcacgt atgtcaaaca aaaatacgtt tttattttga
     3361 gattttatcc aattcacatc atctgttgtg ctatttgaat tgcgtaatac aacggcatca
     3421 aacggattgt tttgccaatt gatagttttt aattcgtcta gattgaatac gtttaaagct
     3481 gttttccaac ctttttgctt aaatcccgca agccaattgg gataattggt gatgagaaca
     3541 aaattgcaat tggaatttcc atcaatgatt ggttgcaaag ccgcaataaa atgtgcttga
     3601 ttcaaaattt gattttcgca cgcgttaaaa tggcgaatgt ctaaataaaa tgtaaacccc
     3661 gtatagtttt caaacggcaa ttcgtccaaa cgggttaatt tttctttgtt ggccgattta
     3721 tagcggattg ttgctaattc gtcagagttt ttactggcaa tgcatcccga agctgttgtt
     3781 gcttcttcta atttttcatc gtgatacaac cacaattgtt ggtcgagtga aagctgaacg
     3841 tctatttcca cccccgatat gccattcaaa cccaaagcca aattgatagc ttgcaaggtg
     3901 ttttcttgat aaatggaatt ttgaatattg agtccattgc ccgcatgtcc caccacttca
     3961 accgatgaaa attgttgttt atcgcaacca aaaaacaagc aaaataacag caaccaacta
     4021 aaacgcatag ctcacatcta tttgaattat ataaccaaag gttccataag tttgcctttt
     4081 attaaccaat gtatgaaaat cattttccca caaaccgccc accatcagcg tttgaccaaa
     4141 gcgccattga ttgcccacat tccaacgcaa gcctcccatc aattcatgaa actgcacaat
     4201 gtcttttgtt gtcgttccaa aacggtagag agaataggta tattgcattc ctaaacccag
     4261 attaatttta ggtttgtgaa ttaaattata aaatccgttg gtttttagtt taggaaaaaa
     4321 tcgtttttga aaaatagagc gattaatgcc gtattcaaat cccacattcc actcaaatgc
     4381 ttggtgttgt tgaccgtatt caaaaccatg aaaaaaatca actttggtta aacttaaaga
     4441 tgtagaaacc cgttgttgcc cccatgaagc aaatgatagg gagaaaataa ataaacaaca
     4501 acaaatagct ttgtgcatga gcagcattta tcggttaaaa ttttggttaa aaatgctaaa
     4561 cctgctgatt tcatcaactg attttttggt tttttacaaa tataaaaaat gacttttgga
     4621 tagactcttt ttaatattaa aaaataaacg gatgaatttg aattgatgtt tgtgaattgg
     4681 caaccccaaa aaaaatctgg ttaaataaat gttaatatta ttacttttgg aacgaaactt
     4741 gagtcaatca attaaattca accaacaaaa tggacattat gaaacacatt ttaactttag
     4801 taattgctgt tgcagcaaca ttttcatttg gacaaaaatt aagattcaaa attgctgacc
     4861 aaaaagacac taccatcttt ttagtgaaat acaatggtgc aaaattatac tatgccgata
     4921 ctgccgaaat gaaaggtgga gtagtggaat ttaatggtgc aaagcaaaaa ccaggtatgt
     4981 tgggcttgtt gtttccgggg caacagttgt tagaatttat ttacaacaat gaggaggtaa
     5041 acattgaggc aaaagggcca aaatatgctg aaacaatggt tgtaaaaaaa tccgaagaaa
     5101 ataaagtttt taacgagtat gtgaaattca tcaccggtaa gcgtatggat gcgcaaaaaa
     5161 ttgccgatga aatgaataaa ttggataaaa aaagtgctga atacaaagaa aaacaaacaa
     5221 aaattgatgc cattagcaaa gaagtattca attttcaaaa caatttggct actgtccact
     5281 ccaataaatt aattggtaaa atggtaaaaa tgaccattga tattgatatt ccagaaccgc
     5341 cagtagacag caaaggcaac atcattgatt ctaattttag ataccattat tttagagatc
     5401 acttttttga taacgttgat tttagtgtag atgggttggt gaatacacct atttttggaa
     5461 ataaaattga atactatttc agcaataaaa tgttgattca acattgggat accattttaa
     5521 aatattccta caatcttatc gataaattga acccaaaatc aaaagcattt gaatacatcg
     5581 tttcttatgt taccagcact tatggtaaaa gcaatcaaat ggggatggat aaagtgtatg
     5641 tgatgatggc tgataaatat tattgctcta aaaatgccga aggaaaatca ccagcttatt
     5701 ggatgaccga agataaattg aaagaattgt gcgaaaaaat tgatgttcaa aaacatttag
     5761 ttatgggggt gaaagctcca aacatcattt tgccagatac gttggataag ccttgggatc
     5821 aattaaattg ggtgaatttg cataaagttg atgccgaata cacggtgttg tatttctggg
     5881 atccagaatg tggacattgt aaaaaagtga caccaaaatt agcaacactt tacgacaaaa
     5941 aattgaaaga cagaaacatt gaagtagttg caattggtaa agctacgggc gaagaataca
     6001 aaaaatggaa agattacatt aagaaaaaca atttgaattt cattaacatc gctgttaccg
     6061 aagctatatt aaaagtagga aaagaagatg ctcgcaagtt gattccacgt tttacaactc
     6121 ttgaatcgat gaatgtatct caaacctatg atattttctc aactccacgt gtttttgtgt
     6181 tggataagga taaaaacttt attgctaaac aaatttctat ttctcaatta gaagaaatgt
     6241 tagaccgttt gcaaggaaaa gcagatttac ctaaattatt cccaccagat aaggaagaag
     6301 acgagcacat gcaaagcaag tagtaggcga taattaccaa tgtaaccatt ttgtagttga
     6361 ttattagtta aaaatatttg taattatttt cacctatttc catatatttg taaaaaaatt
     6421 aatacaaaat atatgagaaa attattactt ctttttattg ctatgcctgc ctttatcttt
     6481 ggtcagggat tcaatgattt tgaatcagtt acattgccaa ctgcttatgg ggatggtagt
     6541 tttactaacg atggcgtaac atatacttat ggtcgcagcc gtgatcaaga tacatatccc
     6601 attgaagata atggtttact gatgcaaaga gcatcggata gttatttaga gtggacagtt
     6661 accaatggaa ttggtcaatt ggattttgaa tacagaaagg cctatacagg taataatgta
     6721 agacagttag aattattggt aaacgggacg caagtggcta cttcacctac ttttgggggt
     6781 gcaagtggag aagatgcaac tatttatact ttttcacatg cagtaaatca agctggaata
     6841 gttactattc gtattaagaa tgtaggtaat acagcaacaa gtagacaaac ggtaatagac
     6901 aacattgctt ggacagatta cactgcacct gcaggatgtg atattacagc ttctggtttg
     6961 gcaaatgtat tgtgcaatga taataatact ggagcagatg caacagatga ttttgtaaca
     7021 tttactttga acccaactgg aaatgattta ggaacaggtg gttataatgt ttctgtaagt
     7081 gctggaacaa ttactccaac aactgctgct tatggtcaag caacaacgtt tacattgcaa
     7141 aatggttcgg ctggagctgg agatgtaact ttaacaatta cagacgttaa tgatggtgct
     7201 tgctcattta cagaaagcat tacagatcca gggacttgtt cttcaacaac acctgtttta
     7261 aatgctgcac ctgcatcgct tacaggtttt aaccatatcg ttggaactcc ttcctcttca
     7321 caaacaatta ctgttgatgg tattgctttg caaggcgatg ttacagttgc tgcacctaca
     7381 aattttgaag tatctttgga taacacgata tatactgcaa gtgttttatt aacgcctaca
     7441 gctggtactt tagcttcaac aaccgtttat gttagagcta atgcagctgt ttatggaact
     7501 ctgtctggaa acttggctgt atcgtcatta aacgcaacta ctgtaaatat tgcattgagt
     7561 ggttttgcag atgattacat ctatcgtaca attgatgaaa ttaacggata cgatgcaaca
     7621 acaggtattg cagattcatt gaataaattg gtgaaaatta caggcgttgt tcaatgtatg
     7681 gactaccgcg caggttctgg ttataacatg gttatcattg atggaactga aaaaggaatg
     7741 tatattttct caactagtga taaaaatggt tatactgacc cagttgcagg tgactctatt
     7801 caagtaaaag gaaaagtaga tcaatttaat ggattaactc aagtgcgtgt tgatgaaatt
     7861 caattattga aaagtggttc tgcaactaca tcgccaacag ttgtaacaat gctagatgag
     7921 tcaacagaaa atcaagttgt tagattggaa aacttaacat ttgttactcc aactgcaacg
     7981 ttcccttctg gaagtgctaa tatggatgtt actgacggta caaatacatt tacaattcgc
     8041 gtgttggctt caatgaactt ggctggaaca gctacaccac aaggtccttt taatgtaagt
     8101 ggtattggtt ctcaattcga tagctctaac cctcatttag caggttatca attgttacct
     8161 tgtggcgctg atgcttttga agaaatttgt acaggtgcta atcttccaga tgtatcagta
     8221 aaccaagcag ccaatacttt aactgcaaac gcatcaactg gtgtaacata ccaatggatt
     8281 aattgtgcaa caaatgcagc aattgctgga gcaacaaatc aatcattcac tgcaacaaca
     8341 ccaggttctt atgctgtagt tgttagcaat gcaacatgtt ctgacacttc aacttgtttt
     8401 acagtttcag atgcttcagt tcaatcaatt gcattggaaa atgccgttaa agcgtatcca
     8461 aatccatttg aaaatgaatt aacattaaca gcttctcaaa cagttcaatt cacaattacg
     8521 gatatgaatg gtaaagtaat tgttgcaact tctgaaatga aagataaagt aacaatttct
     8581 ac
//
LOCUS       DAOQVQ010000049         8536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9202_length_8536_cov_4.224620, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000049 DAOQVQ010000000
VERSION     DAOQVQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_8536_cov_4.224620"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKN22_02660"
     CDS             complement(<1..699)
                     /locus_tag="PKN22_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS41635.1"
                     /translation="MSVFSGYSQHTYPVTKKVQQVDDYFGTKVEDPYRWLEDDRSEAT
                     KAWVIEENAFTANYLSTIPFRETIKNQIKEKWNYEKIGAPFEEGDYTYFYKNNGLQAQ
                     SVLYRKDKNGNEEVFLDPNQFSKNGTTSLSGLSFNKKGNLVAYSISEGGSDWNKIIVL
                     DVATKQQIGDTLVDVKFSGMAWRGDEGFYYSSYDKPDGSELSAKTDTHKVYFHKLNTK
                     QSTAQLVIGEGDFKR"
     gene            850..1257
                     /locus_tag="PKN22_02665"
     CDS             850..1257
                     /locus_tag="PKN22_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNS41636.1"
                     /translation="MTQIKVPFDSIVSSEFQKELQQFTAVKEVAQDEKNQLLITLGSD
                     KKEDISQILNKISEYQADFNFVEAEYPVEKMTCGGCSASAERLLNNQAGVLNARVDFP
                     TKSARVFYKNDVTSPDKLKLALDMLGYDLILDK"
     gene            1276..3477
                     /locus_tag="PKN22_02670"
     CDS             1276..3477
                     /locus_tag="PKN22_02670"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS41637.1"
                     /translation="MAKKTATVTNMTCAACAASVERFLKHQEGVVDASVNFANEQAHI
                     EFSEVTSFDTLKTAVQAIGYDLIATETDEDKKAVASQKENAYKQLKKRVIGALIFAVP
                     LFVIGMFFMHMPYGNYIMWALSTPLVFYFGRQFYVGAWNQLKHRSANMDTLVAMSTGI
                     AYLFSVFNTLFPNYWLSKGIVPHVYFEAAGIVVAFVLLGKLLEERAKGNASSAIKKLM
                     GLQPKTVTIVNQNGAQQEIPIHLIEVNTTVVVKPGERIAVDGEVVSGSSYVDESTING
                     EPVPVSKEKGAKVFAGTINQKGSLHYRAEKVGGDTLLAQIIKMVEEAQGSKAPVQQLV
                     DKIAAVFVPTVIVIALVSFATWMALGGNHGFERGLIAAVTVLVIACPCALGLATPTAI
                     MVGVGKAAEKGILIKDAESIELAKKINVVVLDKTGTITEGKPVVNDVHWFENQTPELL
                     NILFSIEKLSEHPLADAIVAKFKNDAQFLEGMLIENKSGQGISGTFNGTTYFVGNEKL
                     LQEKGQAISSTQRVWVDQQLAKAQTVVFFFDATTLYAAIAISDKMKASSQQAVSQLQA
                     KGIEVVMLTGDNEHTAQVVANQVGISTYKANVLPQDKANFVKEQQANGKIVAMVGDGI
                     NDSNALAQADVSIAMGKGSDIAMDVAKMTIISSDLTKISDAIHVSKQTTRAIKQNLFW
                     AFIYNVIGIPIAAGVLYAYNGFLLDPMWAGAAMAFSSVSVVSNSLLLKFLK"
     gene            3571..5037
                     /locus_tag="PKN22_02675"
     CDS             3571..5037
                     /locus_tag="PKN22_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41638.1"
                     /translation="MKNFKLYQVVLSLLVLIALLITISAFIPEKGINFLGMNLKFPTV
                     DRLLHPKKQQNANIDSLIMDIDTTIVDPLLQHQNGSNGDMGAPNAGELQLKNTTVIYL
                     NDAGRANLDKFFEKLETAARDKKKIHILHYGDSQIEGDRMTGFIRERLQNQFGGNGPG
                     LVPANNVYNTLAFIQKYSENFIRYTCFGGEKLKNRKYGVMGSAARFTKEFSDSIEIAN
                     EKTQKTAWIEIAASPSAYGRSRNFNTVKMYYNSCIKPCAVNVYENGTLIHQDSLLTDG
                     KAHILKLTFPKTPGTLKYEFKSAISPTISNFSLEGDYGIQMSNIGMRGSSGTIFGSMD
                     KGITAEAMSDLGTELIIMQFGGNSVPSFKDSSGVRNYARYFKGQLNTLKSMRPSAAIL
                     VIGPSDMSMMTEGVYETYPLLPYCVEQMKKLTLEAGAGYWDLFEAMGGVNSMPAWVAK
                     GLAGSDYVHFSNGGARIAAQKFYDALIAEYIRWSGKDN"
     gene            5040..6365
                     /locus_tag="PKN22_02680"
     CDS             5040..6365
                     /locus_tag="PKN22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNS41639.1"
                     /translation="MYSIFLKSLIGLAIVLTGFVGFAQKQQPLNNYAYPDFILQDTAL
                     CSLYDFVDFSKNNFHFYTENSPNFEYLYHQLDSMKRFQDRKLNFYHIGGSHIQADVYS
                     NDIRLFLQTSNIDLPGERGWVFPLNLAGTNNPASYRITSPNYFKGYRVVGNKDKTIDF
                     GTMGALVTCADSVVHLKFEHKNTTSKPGFCKIKIFHNKGDFPYELNFGNDEILVVQTI
                     HHPEIGYTEIEFTDKIDSVNVYFSRTVSQRIELELYGFQLLNEDPGISYNTIGINGAG
                     LYSYLDCNRFEEQLKTYPPDFFAFSVGTNDANMPYDAFKPEVYRNNLEKMMQIALRAN
                     PHCALLLTVPNDAYYKRKYLNRNVAREREMIIELAKKYKMAVWDFYGIMGELGSSKIW
                     MHKSLMRTDLVHFTSPGYHFKGELFIDAYLKFLLQMKEYFAVDTAAVKE"
     gene            complement(6382..7143)
                     /locus_tag="PKN22_02685"
     CDS             complement(6382..7143)
                     /locus_tag="PKN22_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41640.1"
                     /translation="MKQILIFLIFTNILTTFSYSQIQIGGDEKAAKKEKKKKEKVKPV
                     EKDFYDFRVYVSGTLGSSYRLLKPNTKNPLFMDSLGQRANETRSMGWGTTLGFTSDLS
                     KYLMWEAGFSYFQNSEKYSYQATDTSHTYTSKYTWIGIPLKLHFKYDLKKVRFNVGAG
                     VLPQMQFKYKQDDEFVNHNGKTTTSSVKTIKDVNTFGISVVANAGIHYSFTKRFGAFL
                     QFEYRQQLTPSHLKTYPYIHKGNNIGATFGFTLGF"
     gene            7200..>8536
                     /locus_tag="PKN22_02690"
     CDS             7200..>8536
                     /locus_tag="PKN22_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNS41641.1"
                     /translation="MDTSEKKKKKSIVKRILKWSGISFLLLLLAIILIPIFFKDKIKD
                     LVLEEVNKTLTAKVELDEFDLTFLSTFPNLSVQLDGVRVKGTGDFDGVTLADVKTLRA
                     DVGFWDVVKGETISIDAIYLNEPKVDVRVLYDGRANFDIVKPDSLKTEEEKSEPSNFK
                     LELKKYAIENADVRYDDKQGGLYANIVKLNHIGKGDLTADVVDFETKTTADAITFEMG
                     GLSYLSKVKTDIVANLLLEFNENASKFTLKENTFQLNALKFGIDGFYEQLADKANMDL
                     ALKADKATFKEFISLIPAFYRTGYEGMLASGSLALNAKVKGVLDDTNMPGWDAGIKVA
                     NASIKYPDLPGKINNIAIVAKSQFAGGSNLDKMTVDVDKFHADFVGNVVDATLKLRNV
                     MTDPAIASTLLAKVDLATLGKVIPLAEGETYNGKLDADVSLDGRMSYIEKENY"
BASE COUNT         2710 a         1490 c         1706 g         2630 t
ORIGIN      
        1 gcgtttaaaa tctccctctc caatcactaa ttgggcagtt gattgtttgg tattcaattt
       61 atgaaaatac actttgtgcg tatccgtttt tgccgagagt tcacttccat cgggtttgtc
      121 gtagctggaa taataaaatc cttcatcgcc tctccaagcc attccagaaa attttacatc
      181 caccaacgtg tcgccaattt gctgcttggt tgcaacatcc aaaacaatga ttttattcca
      241 atcactgcca ccttcagaaa tagaataggc cactaaattg ccttttttat tgaacgacaa
      301 tccactcaac gaagtagttc cattcttaga aaattgattg gggtccaaaa acacctcttc
      361 atttccgttt ttatcttttc gatacaaaac agattgtgct tgcaaaccgt tgtttttata
      421 gaaatatgta taatcacctt cttcaaatgg agcacctatt ttttcgtaat tccatttttc
      481 ttttatctga tttttaatcg tttcacgaaa aggaatagtt gacaaataat tagctgtaaa
      541 agcattttct tcaattaccc acgctttagt agcttcagat cggtcgtctt ccaaccaacg
      601 ataaggatct tctacttttg ttccaaaata atcatctacc tgttgcactt ttttggtgac
      661 aggataggta tgttgcgaat aacctgaaaa tacactcatt gtaaatgttg ctataattaa
      721 ttgtttgttc attttccttt tttttcaaag ataggcattt gccagtaaaa ttgtaaacta
      781 ttctcaaaaa gttataagaa ctttgcggtg caatgtgttt acctttgcaa aaaattaatt
      841 caacacatta tgacacaaat taaagttcca ttcgatagca tcgtttcttc tgagtttcaa
      901 aaagaattgc aacaatttac agctgtaaaa gaagttgctc aagacgaaaa aaatcaatta
      961 ttgattacct taggaagtga taaaaaagaa gatatttcgc aaatattaaa taaaataagc
     1021 gaatatcaag cagattttaa ttttgtagaa gcagaatatc cggtagaaaa aatgacctgt
     1081 ggaggatgct ctgcaagtgc cgaacgttta ctcaataatc aagctggagt tttaaatgca
     1141 cgtgtagatt ttccaacaaa atcggctcgt gttttctata aaaacgatgt tacttcgcct
     1201 gataaattga aattggcgtt ggacatgctg gggtatgatt tgatactcga taaataataa
     1261 aattcttctt cattcatggc aaaaaaaaca gcaacagtca ccaatatgac atgtgcggcg
     1321 tgtgctgcaa gtgttgaacg atttttgaaa catcaagaag gggtagtgga tgcatcggtc
     1381 aactttgcaa acgaacaagc ccacattgaa ttttcagaag ttacttcgtt tgacacttta
     1441 aaaacagcgg tgcaagccat tggatatgat ttaattgcca cagaaaccga tgaggataaa
     1501 aaagcagtag cttctcaaaa ggaaaatgcc tacaagcaat taaaaaaacg cgtgattggt
     1561 gcgctcattt ttgctgtccc tttgtttgta ataggtatgt tttttatgca catgccttat
     1621 ggaaattata tcatgtgggc actttcaacg ccactagttt tttactttgg aaggcaattt
     1681 tatgtgggtg catggaacca attgaaacac cgttcggcca atatggatac tttggtggca
     1741 atgagcaccg gaattgctta tttgtttagt gtttttaata ccttgtttcc taattattgg
     1801 ttgagcaaag gcattgttcc acacgtgtat tttgaagcag ctgggattgt ggttgctttt
     1861 gttttacttg gaaaattatt ggaagaacgt gccaaaggaa atgccagttc tgcaatcaaa
     1921 aaattgatgg gtttgcaacc aaaaacggta accattgtca accaaaatgg tgcacaacaa
     1981 gaaattccta tccatttgat tgaggtgaac acaacggtgg ttgttaaacc tggagaacgt
     2041 attgccgttg acggagaagt tgtcagtggg agctcgtatg ttgatgaaag taccattaac
     2101 ggcgaacctg ttccagtttc taaagaaaaa ggggcaaaag tttttgcagg caccatcaat
     2161 caaaaaggaa gtttgcacta tcgagcagaa aaagtgggcg gcgatacgtt gttggcacaa
     2221 atcatcaaaa tggtggaaga agcacaagga agtaaagcgc ctgtgcaaca attggtggat
     2281 aaaatcgcag ccgtttttgt tccaaccgtt attgtgattg cattggtgag ttttgcaaca
     2341 tggatggcac ttggaggcaa tcatggtttt gaacgtggat tgattgcagc ggttactgtt
     2401 ttggtgattg cttgtccttg tgcgttaggt ttggccactc caacggctat catggtggga
     2461 gtaggcaagg cggccgaaaa aggaatttta atcaaagatg ccgaaagtat tgaattggca
     2521 aaaaaaatca atgtggtggt gttggataaa acaggaacta tcaccgaagg aaagccagta
     2581 gtgaatgacg tgcattggtt tgaaaatcaa actcctgagt tgttgaatat tctgttttcc
     2641 attgaaaaac tatcagaaca tccattggca gatgctattg ttgcaaaatt caaaaatgat
     2701 gctcaatttt tagagggaat gcttattgaa aacaaaagtg ggcaaggcat cagtgggact
     2761 tttaatggga cgacgtattt tgttggaaac gaaaaacttt tgcaagaaaa aggtcaagca
     2821 atttcatcaa cacaacgagt gtgggtagac caacaattgg caaaagcaca aactgttgtt
     2881 ttcttttttg atgcaacaac tttgtatgct gcaattgcca tttcggataa aatgaaagca
     2941 agttcacaac aagcggtgag ccaattgcaa gcaaaaggaa tagaagtggt aatgttgacc
     3001 ggtgataatg aacacacagc acaagtggtc gccaatcaag tgggaatttc tacttacaaa
     3061 gccaacgtat tgccgcaaga caaagctaat tttgtgaaag aacaacaagc caatggtaaa
     3121 attgttgcca tggtgggtga tggtattaac gatagcaacg ctttggcaca agcggatgtg
     3181 agtattgcca tgggtaaggg aagcgatatt gcaatggatg tagccaaaat gaccattatt
     3241 tcttccgatt taaccaaaat ttcagatgct attcacgttt ctaagcaaac aacacgtgcc
     3301 attaaacaaa atttattttg ggcatttatt tacaacgtta ttggaattcc aattgctgcc
     3361 ggtgtgttgt atgcctacaa cggattttta ttagacccta tgtgggctgg agcagccatg
     3421 gcgtttagtt cggtaagtgt tgtttccaat agtttattgt tgaaattctt aaaataaacc
     3481 gataattttt ggtttatctt gcttgatttt gagttatcgt taacaattta tccgttgact
     3541 tcgctatttt tgtagctttc aaattgcaag gtgaaaaatt ttaaattata tcaagttgtt
     3601 ttatcgcttt tggtgttgat tgccttattg ataaccatta gcgcatttat tccagaaaaa
     3661 ggaataaatt ttttggggat gaacctaaaa tttccaactg ttgatcgttt gttgcaccct
     3721 aaaaaacaac aaaatgccaa cattgatagc ttgattatgg acattgacac aaccattgtt
     3781 gatccacttc ttcaacacca aaatggttct aatggcgata tgggcgcacc caatgctggt
     3841 gaattacaac taaaaaacac taccgtcatt tacctcaatg atgcgggaag agccaatttg
     3901 gataaattct ttgaaaaatt agaaacagca gcccgagata aaaagaaaat tcacattttg
     3961 cattatggcg attcacaaat tgaaggcgac cgtatgacag ggtttattcg tgagcgttta
     4021 caaaatcaat ttggtggaaa cggaccaggc ttagtgcctg ctaataatgt ttacaataca
     4081 cttgcattta ttcaaaaata ttctgaaaat tttattcgct acacctgttt tggaggtgaa
     4141 aagttgaaaa atagaaaata cggtgtaatg ggttctgcgg ctcgttttac caaagaattt
     4201 tcggatagca tagaaattgc aaacgagaaa acacaaaaaa cagcttggat agaaattgcc
     4261 gcttctccaa gtgcttatgg tcgctcacgc aattttaaca ccgtaaaaat gtactacaac
     4321 agttgcatca aaccgtgtgc tgtcaatgtg tatgaaaacg gaacgttgat ccaccaagat
     4381 agcctactca cagatggcaa agcgcatata ttaaaactga cttttccaaa aactcctgga
     4441 actttgaaat acgaatttaa aagtgcaatt agtccaacca tcagtaattt ttcattggaa
     4501 ggcgattacg gtattcaaat gtcaaatatt gggatgcgtg gttcaagtgg gacgattttt
     4561 gggtcaatgg acaaaggtat aacagccgaa gccatgagtg atttaggcac cgaactcatc
     4621 attatgcaat ttggtgggaa ttctgttccg tcgtttaaag acagctcagg agtgcgtaac
     4681 tatgcgcgtt actttaaagg acaattaaac accctgaaat ccatgcgacc atcagcggct
     4741 attttagtaa ttggtccgag tgatatgtct atgatgaccg aaggtgttta tgaaacctat
     4801 ccattgttgc cctactgtgt ggaacaaatg aaaaaactta cactcgaagc aggtgctggc
     4861 tactgggact tgtttgaagc tatgggcggc gtaaattcaa tgcctgcatg ggtggcaaaa
     4921 ggtcttgccg gaagcgatta tgtgcatttt agcaatggtg gcgcgagaat tgcagctcag
     4981 aaattttacg atgcgcttat tgccgagtac atccgttgga gtggaaaaga taattaagaa
     5041 tgtatagcat ttttcttaaa agtttaattg ggttagccat tgttctaact ggttttgttg
     5101 gctttgcaca aaagcagcaa ccactcaata attatgcata tcccgatttt attttgcaag
     5161 atactgcact ttgctccttg tatgattttg ttgatttttc taagaataat tttcatttct
     5221 acaccgaaaa cagccccaat tttgagtacc tctaccacca attggattcc atgaagcgct
     5281 ttcaagaccg caagttgaat ttttaccaca ttgggggttc acacattcag gcagacgttt
     5341 attctaacga cattcgcttg tttctacaaa cttctaatat cgatttacca ggagaaagag
     5401 ggtgggtgtt tccgctcaat ttggcgggaa ccaacaatcc ggcaagttac cgcattacct
     5461 ctcccaatta tttcaaaggc tatcgtgtgg tgggaaacaa agataaaacc attgattttg
     5521 gaacaatggg agctttggta acgtgtgccg attctgtagt tcatctaaaa tttgaacaca
     5581 aaaataccac ttcaaaaccc ggtttttgca aaatcaaaat ctttcacaat aaaggcgatt
     5641 ttccttatga gctcaatttc ggcaacgatg aaatattagt tgttcaaacc atacatcatc
     5701 cagaaattgg ctatacagaa attgaattta ccgacaaaat tgattctgtt aacgtttatt
     5761 tttcgcgcac tgtttcgcaa agaatagagt tggagttgta tggatttcaa ttgctcaacg
     5821 aagatccggg gatttcatac aataccatcg gcatcaacgg tgcaggtttg tattcctatt
     5881 tagattgcaa ccgttttgaa gaacaattaa aaacctatcc acccgatttt tttgcctttt
     5941 cagttggaac caatgatgcc aatatgccct acgatgcgtt taaacccgaa gtatatcgta
     6001 ataatttaga aaaaatgatg caaattgcct tgcgtgccaa tccacattgt gcgttgttgc
     6061 taaccgttcc aaacgatgct tattacaaaa gaaaatacct caatcgcaac gtggcacgtg
     6121 aacgtgaaat gattattgaa ttggctaaaa aatacaaaat ggccgtgtgg gatttttatg
     6181 gcattatggg cgagttagga tcctcaaaaa tttggatgca caaaagctta atgcgtaccg
     6241 acttagtgca ttttacttcg cccggctacc atttcaaagg cgagttgttt attgatgcct
     6301 atctgaaatt tttactgcaa atgaaagaat attttgcagt ggacacagcc gccgtaaaag
     6361 aataaacgtt tcaatttttt cttaaaatcc taaagtaaat ccaaaagtgg cgcctatgtt
     6421 gttgcctttg tgaatgtagg gataggtttt cagatgactt ggagttaact gctgacggta
     6481 ttcaaattgt aaaaaagccc caaaacgctt ggtaaaacta tagtgaattc cagcattggc
     6541 aaccactgaa ataccaaaag tgtttacatc tttaatcgtt tttacagagc ttgttgtggt
     6601 tttcccattg tgatttacaa attcatcgtc ttgcttgtat ttaaactgca tttgaggcag
     6661 aactccagca ccaacgttga aacgcacctt ttttaaatcg tatttaaaat gcaatttaag
     6721 tggaatgcct atccaagtgt attttgaggt gtaggtgtga ctagtatctg tagcctgata
     6781 actgtatttt tcactgtttt gaaaataaga aaaccctgcc tcccacatca aatatttcga
     6841 taaatcactg gtaaatccta aagttgttcc ccaacccatg ctgcgtgttt cgttggcacg
     6901 ttgacccaag ctatccataa acaaaggatt tttggtgtta ggttttaaaa gtcggtaact
     6961 gcttcccaaa gttccactta cgtaaacacg gaaatcataa aaatcttttt ctaccggctt
     7021 cactttttct tttttctttt tttccttctt agcagccttt tcgtcacccc caatttgaat
     7081 ttgactataa ctaaaagttg ttaaaatatt tgtaaaaatt agaaatatca aaatctgttt
     7141 cataaaaatc tgttagattt acctattatt tttcaaaaat atcaaaaaaa cacagcttaa
     7201 tggatacttc agaaaagaaa aaaaagaaaa gtattgttaa acgcatcctc aaatggtcgg
     7261 ggattagttt tctactatta ctccttgcaa ttatcttaat tcctatcttt tttaaagata
     7321 aaattaaaga tttggtgcta gaggaagtca acaaaaccct tactgccaaa gtagagttag
     7381 atgaatttga cttgacattt ttaagcactt tcccgaattt atcggtacag ttagacggtg
     7441 ttcgtgtaaa aggaacagga gattttgatg gggtaacctt ggcagatgta aaaacgcttc
     7501 gtgccgatgt tggtttttgg gatgttgtaa aaggcgaaac catttctatt gatgccattt
     7561 atttaaacga accaaaagtg gatgtgcgtg tattatatga cgggcgtgcc aattttgata
     7621 ttgtaaaacc cgattctctt aaaacagaag aagaaaaaag tgaaccttcc aacttcaaat
     7681 tggaattgaa aaaatatgcc atagaaaatg ccgatgtgcg ttacgatgat aaacaaggtg
     7741 gtttgtatgc caatatcgta aaattaaatc acattggaaa aggtgattta actgccgatg
     7801 tggttgattt tgaaactaaa acaacagccg atgccattac ttttgaaatg ggaggtttgt
     7861 cgtatttaag caaagttaaa accgatattg ttgccaattt gttgttagaa ttcaacgaaa
     7921 atgcatctaa atttaccttg aaagaaaata catttcagtt aaatgcgtta aaatttggaa
     7981 ttgatggttt ttacgagcaa ttagccgata aagccaacat ggatttggcg ttaaaagccg
     8041 ataaagctac atttaaagag tttatttctt taattccggc gttctacaga acaggatacg
     8101 aaggtatgct ggcatcaggg tctttagcat tgaacgcaaa agtaaaaggg gtgttggatg
     8161 ataccaacat gccaggttgg gatgccggaa ttaaagtagc taatgcttct attaaatatc
     8221 ccgatttacc aggtaagata aataacattg ccattgttgc taagtcacaa tttgctggtg
     8281 gaagcaattt ggataaaatg acagtggatg tggataaatt tcatgctgat tttgttggaa
     8341 acgtggtaga tgcaacgtta aaattgcgca atgtgatgac cgatccagct attgcttcaa
     8401 cattattggc aaaagtagat ttggctaccc ttggaaaagt tattccactt gcagaaggag
     8461 aaacctataa cggtaaattg gatgccgatg ttagcttgga cggacgaatg agctacatcg
     8521 aaaaagaaaa ctacga
//
LOCUS       DAOQVQ010000050         8479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9286_length_8479_cov_3.900166, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000050 DAOQVQ010000000
VERSION     DAOQVQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9286_length_8479_cov_3.900166"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PKN22_02695"
     CDS             <1..584
                     /locus_tag="PKN22_02695"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNS41642.1"
                     /translation="RFTFLGITSPIRKELQKPFLAKQNLPDKKEGFEVVRLLWQQPER
                     EFHYFALELLLAYKKHYEIADIDFFEQLILSNSWWDSVDYLSPKIVAAYCQKFPAMQE
                     KIAHKWMKSENIWLKRSALLFQLKYKNQLNAELLKTIILSMHTTNEFFINKAIGWILR
                     EYARTNPQWVLDFVATHPLSNLSNREALKHLHS"
     gene            910..1725
                     /locus_tag="PKN22_02700"
     CDS             910..1725
                     /locus_tag="PKN22_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNS41643.1"
                     /translation="MAKSKKIEIGGKEISIISQKQDDYISLTDMAKSQMQEAIIIKWL
                     TLKSTIEYIGEWEALYNPDFNYTEFGTIKNSAGSNNFVLSVKNWIEATNAIGITAKAG
                     RYGGTYAHKDIAFHFGMWISPKFQLLLVKEYQRLKEDENNRLQLEWNIQRTLAKVNYQ
                     IHTDAIKENLIPQEITKQQANFVYANEADLLNVALFGITAKEWRDKNQDKTGNIRDYA
                     TLEQLVVLSNMESINALLIRQGLTQPERLIQLNKVAITQMKSLLESNAFKKLK"
     gene            1916..2296
                     /gene="msrB"
                     /locus_tag="PKN22_02705"
     CDS             1916..2296
                     /gene="msrB"
                     /locus_tag="PKN22_02705"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNS41644.1"
                     /translation="MEEMDWKAKLSPEQYEILRNKGTERPFTGRFNDFFEAGEYVCAG
                     CGEPLFLSNQKFHSGCGWPSFDAAIEGKIEETPDYSHNMIRTEITCKKCGGHLGHVFN
                     DGPTKTGLRYCVNSISLDFIQGEE"
     gene            2427..4133
                     /locus_tag="PKN22_02710"
     CDS             2427..4133
                     /locus_tag="PKN22_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS41645.1"
                     /translation="MTLDSHYDIVIIGAGISGLTSAALYSRLGYRVCVLEMDARPGGY
                     IAGFNRLEYRFDSAIHWLNQCGADGLVTQAFSLIGSDYPKTKTQHKIKTILSDQYQIA
                     LTTDVQQLEKQLIHDFPEDTNGIKRFFRQAKKIGASFQKFRNINRSFATMQPLEKARR
                     GLKMLQFALPFIPHIRYSGEKIDKGLQNYFTNPQLLGLFKSEPDLLSCLIPIGWASIH
                     DYQLQPEGGSQTFAEWLEHVCAEYQTPIHYKSKVVAINSQGNKATGVEYVCKNEVKKV
                     TCNYVIAACDAQYLYKNLLPQSQYTQAFLDKINQSELYASAFTISLALDCTGESLGFD
                     ESMLFICDDGLGREAYSDGNPATCGIHVMSGSVRDKSLAPEGKGTLTIFIPSYIDQYN
                     YWETEKVGDHYKRGEAYKKTKNEVALTIIKRIEKRLKIDLQSHIVYLDTATPVTHFRY
                     TGNKNGTMMGTRPGKKNMQAKVAQYKTPLDNVFLSGHWAELGGGIPIAVKAAMNSTLL
                     VLKKDNKHAFRMLANYMDGKIDRQEFEDSKLLLPYSNSWKRALTPAEKAQLKSEKFKN
                     SI"
     gene            complement(4297..4863)
                     /locus_tag="PKN22_02715"
     CDS             complement(4297..4863)
                     /locus_tag="PKN22_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNS41646.1"
                     /translation="MSKELSIKNMVCDRCIMAVKNTLEQLQIDYSSVTLGKTELTQPI
                     SDEKLNQLEHQLQQIGFELLKDKKIQLVEKVKNLIINHVHHQHTTLKVNLSDYLSDEI
                     SMDYGSISKLFSEMEGTTIEKFVIAQKIEKVKELISYNELTLSEIADELNYSSVAHLS
                     TQFKKVTGLTTTEYKLNNQNDRKPISGI"
     gene            complement(4873..6270)
                     /locus_tag="PKN22_02720"
     CDS             complement(4873..6270)
                     /locus_tag="PKN22_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS41647.1"
                     /translation="MRAQADRKVIRGWIFYDWANSVYNLVISSAIFPIFYDNVTTKAF
                     LKNYNAANNLNLTEVPANVDVVVPFFGTQLSPSVLMSFTLSISFLVVSILSPMLSGIA
                     DVTGSKKRFLQFFCYLGALSCVSLYWFGKAPFEIGMLSVFLASIGFWNSLVFYNAFLP
                     EIAVPRLHDKISASGFTMGYLGSMLLLIICLAIIMGIGPEYTGVSFILVGVWWVSFSQ
                     ITFRRLPNNVYNKAAVPNKLTRGFKELKNVFFEFNQSKLLKRYLLSFFFFNTGVQTVM
                     LMAVLYAKIEIFSGEYADQATTGLILAILLIQLLGAFGAFILSRVSNVLGNIRSLIIA
                     VAIWVLLCLWAYFIQTPTEFYYLAAGVGLVMGGTQALARSTYSKMLPRTENHASYFSF
                     YDVAEKIGIVFGTLFFGLMEYLTNDIRSSILSVIFFFSIGLIALFFVPISNKRNDNDY
                     DKQLTQDNPTFEFLK"
     gene            complement(6267..7499)
                     /locus_tag="PKN22_02725"
     CDS             complement(6267..7499)
                     /locus_tag="PKN22_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS41648.1"
                     /translation="MKQVRIGIVREGKIPRDLRVPLTPKQCKTIEAQYPEVEVIVQPS
                     PFRSYKDEEYRAEGIALNEDLSACDVLLGVKEVNISDLIPNKKYMFFSHTMKKQAYNQ
                     KLLRAILDKKIQLIDYEALKDKNNKRIIGFGRYAGIVGTYNGFLTLGLKTKTFNLKPA
                     YLCEHREELEKELVNVSLPANTKIVVTGWGRVGNGAKEILDLLPIKEVSPREYLEETF
                     DEPVYTHLDTEDYYAPKNGGEFVKKDFYSAPETYKSTLDTYILKDTTMYIPCHFWSNR
                     APKLITTELLTNPKCKLKVIADVSCDIADPIVSTIRPSKIGDAIYGYNPFTAKEVDFM
                     DEDAVAVMAVDNLPCELPKDASEDFGNELIRSVFPRLFREDPDQIIFRGSETDLNGNL
                     TSHFEYLSDYANTPNTVE"
     gene            complement(7527..8198)
                     /locus_tag="PKN22_02730"
     CDS             complement(7527..8198)
                     /locus_tag="PKN22_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNS41649.1"
                     /translation="MMLDENIKGLCSKYGLDHVAFLADFGIENVQELTLFDLQAIAEE
                     YEVDLYALLFKPLFPNQHLKDKLKKIKLLVLDVDGVMTDGGMYFTESGDQFKKFNTKD
                     GMAIIQLTKNNFQIAIISSGFVSQSVEARAKMLGIQHCVVSRDKKIDVLQQIIEKLNL
                     SLAEVAIIGDDINDLDIMKSVSIAACPKDAVQEVKNNCHIVLNTNGGRGCVREFIDLH
                     LIDKI"
     gene            complement(8202..>8479)
                     /locus_tag="PKN22_02735"
     CDS             complement(8202..>8479)
                     /locus_tag="PKN22_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNS41650.1"
                     /translation="FTLDLCGVAWLQEYTNLPIILDPSHAIGYAYGVLPLAKACVAMG
                     VDGLLIEAHPNPKVAKSDAAQQLNHQEFTALKNELQTVAQAVGYKIV"
BASE COUNT         2806 a         1590 c         1506 g         2577 t
ORIGIN      
        1 atcgttttac ttttttaggc atcacctcgc ccattcgcaa agaactacaa aaaccatttt
       61 tagctaaaca aaatttaccc gataaaaaag aaggttttga agtggtacgt ttattatggc
      121 aacaacccga aagagagttt cattattttg cattggaatt attgttggcc tacaaaaaac
      181 actatgaaat tgccgatatt gatttttttg agcaattgat tctatctaac tcttggtggg
      241 acagtgtgga ttatctttca cccaaaattg tagctgccta ttgccagaaa tttcctgcaa
      301 tgcaagagaa aattgctcac aaatggatga aatccgagaa tatttggttg aaacgttctg
      361 ctttgttgtt tcaattaaaa tacaaaaacc aactcaatgc cgagctattg aaaacaatta
      421 ttctttccat gcacactacc aatgagtttt tcataaacaa agcaataggt tggattttga
      481 gagaatatgc tcgaaccaat ccgcaatggg tgcttgattt tgtggcaacg catccgttgt
      541 cgaatcttag taatcgagaa gctttaaagc acctccattc ctaacacatc ttcttaaaat
      601 tttgtaattt taagctatgg aagagatgga ttggaaagca aaatgattta tgaaatcatt
      661 gatgacaaca taagacaata gagttttata acttaatcgg atattgttca aacttacaat
      721 tccaaatccc taaaaccttt taatttttaa tagttttagg aatttcacac atctttgtga
      781 tatgaaaacg atactacgaa aagttattta gacatacttt ctgtattgca atagattgtc
      841 tattgaacgc taaaaaataa gggctaccgc taacattttt gtaaatttat catttattaa
      901 aaagaaaata tggcaaagag caagaaaata gaaattgggg gtaaagaaat aagtattatc
      961 tctcaaaagc aagatgatta tatttcgctt acagatatgg caaaaagcca aatgcaagaa
     1021 gctatcatca taaaatggct gactttgaaa agtaccattg aatacattgg cgaatgggaa
     1081 gcattgtaca atccggattt taattatacc gaattcggta caattaaaaa ttcggcaggt
     1141 agcaataatt tcgttctttc cgtgaaaaat tggattgaag caactaatgc aattgggatt
     1201 actgccaaag caggaagata tggaggaacg tatgcccaca aagacatcgc atttcatttt
     1261 ggaatgtgga ttagtcccaa gtttcagttg cttttagtaa aggaatacca acgacttaaa
     1321 gaagatgaaa acaacagact gcaattagaa tggaacatac aacggacttt ggcaaaagtc
     1381 aattatcaaa tccatacaga cgcaatcaaa gaaaatctga taccgcagga aattacaaag
     1441 caacaagcca attttgttta tgccaacgaa gctgacctac tgaacgtggc actttttggt
     1501 attacagcca aagaatggcg agataaaaat caagacaaaa ctggaaacat cagagattat
     1561 gcaacattag aacagttggt tgttttaagc aatatggaaa gtatcaatgc tttgcttatt
     1621 cgacaaggtt tgacacaacc cgaacggctg atacaactca acaaagttgc tataacgcaa
     1681 atgaaatcgt tgcttgaaag taacgcattt aagaaactaa aatagacaga cgaacaacaa
     1741 cgaaccgcta ttatataaca ctcaaccact tcgaagtgca attgcggttt caatttttgt
     1801 taccaaagtg gagctgtata gtccaacttt ggagcttcta taaaattaag tggtttctaa
     1861 cctgaaaaca cctccattcc taacacatct tcttaaaatt ttgtaatttt aagctatgga
     1921 agagatggat tggaaagcaa aattaagccc cgaacaatat gaaatattgc gcaacaaagg
     1981 tacggagcga ccttttacgg gtaggttcaa tgattttttt gaagctggag aatacgtgtg
     2041 tgccggttgt ggcgaaccgc tttttttaag taatcaaaaa tttcattcgg gttgtggatg
     2101 gccaagtttt gatgcagcca ttgaaggaaa aattgaagaa acacctgatt actcgcacaa
     2161 tatgatacgc acagaaataa cgtgtaaaaa atgtggcgga catttgggac atgtgtttaa
     2221 cgatggtcca accaaaacag ggttgcgtta ttgtgtgaat tctatttcat tggatttcat
     2281 ccaaggagaa gaataaatgc gtggtggttt tgcacaaaaa atcataaata tttaattatc
     2341 aaaccaattt ctaaattatg tttttatctt taaagtcgtg caattgattt gtacacttaa
     2401 aacaacacaa aaacattcaa aagaaattga ctctagattc ccattatgac attgtaatca
     2461 tcggtgctgg tattagcgga ttaacgtcgg ctgcgctgta tagtcgcttg ggttatcgcg
     2521 tttgtgtgtt ggaaatggat gcgcgccctg gtggttacat tgcaggattt aaccggttgg
     2581 aatatcgttt tgattctgcc attcattggc tcaatcaatg tggagcggat ggattggtaa
     2641 cgcaagcctt ttcgttaatt ggcagcgact atccgaaaac caaaacccag cacaaaataa
     2701 aaacaattct atcggatcaa tatcaaattg cattaacaac cgatgttcag cagttggaaa
     2761 agcaattgat tcatgatttt ccggaagata caaatggcat taaacgattt tttcgacaag
     2821 caaaaaaaat aggtgcatcg tttcaaaaat ttaggaacat caatcgcagt tttgcaacta
     2881 tgcaacctct tgaaaaagca aggagaggtt taaaaatgtt gcaatttgcc ttgcctttta
     2941 ttcctcacat ccgatattcg ggagaaaaaa ttgataaagg gctacaaaac tattttacca
     3001 atccacaatt gttaggtttg ttcaaatcag aacctgattt attgtcgtgt ttaattccca
     3061 ttggttgggc ttccattcac gattatcaat tgcaaccaga aggtggaagt caaacgtttg
     3121 cagaatggtt ggagcatgtg tgtgctgaat accaaacgcc catacattac aaatcaaaag
     3181 tggtggcaat taattcacag ggcaacaaag ctactggcgt tgaatacgtt tgcaaaaacg
     3241 aagtaaaaaa agttacctgc aattacgtca tagcggcttg tgatgcgcag tatttgtaca
     3301 aaaacttatt gcctcaaagt caatatacgc aagcattttt agataaaatt aatcaatcgg
     3361 agttgtatgc ttcggcgttt accatttcat tggcattgga ttgcacggga gaatcgttgg
     3421 gttttgacga atcgatgttg tttatttgcg acgacggttt gggtagagaa gcctatagtg
     3481 atggcaatcc tgcaacgtgt ggcattcatg tgatgtcggg ttctgtgcgc gataaaagcc
     3541 tagcaccaga aggaaaagga acgcttacga tttttattcc ttcctatatt gatcaataca
     3601 attattggga aaccgaaaaa gttggcgacc actacaagcg gggcgaagct tataaaaaaa
     3661 caaaaaatga ggttgcttta accatcatta agcgcattga aaaacgatta aaaattgacc
     3721 tgcaatcgca cattgtctat ttagacacgg caacacctgt tacacatttc cgctatacgg
     3781 gcaataaaaa cggtacgatg atgggcactc gtccgggcaa aaaaaacatg caagcaaagg
     3841 tggcgcagta taaaacacca ctggacaatg tgtttttgag cggtcattgg gcagaattag
     3901 gcggtggtat tccaattgct gtaaaagcgg cgatgaattc aactttattg gttttgaaaa
     3961 aagacaataa acacgcattt cggatgttgg ccaactacat ggatggaaaa attgaccgtc
     4021 aagaatttga agattctaag ctccttcttc cctattccaa ttcgtggaaa agagcgttaa
     4081 caccggcaga aaaagctcaa ctaaaaagtg agaaattcaa gaatagcatc tagatgttat
     4141 cattttagtc acctccctta atccctcctc aaggagggaa atgcaaccga actcaagacg
     4201 acgaataatc tacaagaatt aaaagaagta aagttatttt tggacgatac aaattacggg
     4261 tgtcacctcc cttaatccct cctcaaggag ggagagttaa atcccgctaa ttggttttcg
     4321 atcgttttgg ttgttgagtt tgtattcggt tgtagtaagt cctgtaactt ttttgaattg
     4381 tgtgcttagg tgtgcaacgc tggagtagtt taattcatcg gcaatttcgc tcaatgtcag
     4441 ttcgttgtag ctaatcaatt cttttacttt ttctattttt tgggcaatga caaacttctc
     4501 gatggtggtt ccttccattt cagaaaacaa tttgctgatg gaaccataat ccatagaaat
     4561 ttcatcgctt aaataatccg acagattaac tttcaacgtt gtatgttgat gatgcacatg
     4621 gttaataatc aagtttttga ctttttctac cagttgtatt tttttatctt tcaataattc
     4681 aaaaccaatt tgttgcaatt gatgttccaa ttgattcaac ttttcgtccg aaatgggttg
     4741 tgtaagttcg gttttaccta aagtcaccga cgaataatca atctgcaatt gctccaaggt
     4801 atttttaacc gccattatgc agcgatcgca caccatgttt ttgattgaaa gttctttaga
     4861 catgtcgttc aattacttta aaaattcaaa cgttggatta tcttgcgtta attgtttatc
     4921 ataatcatta tcgtttcttt tattgctaat tggcacaaag aaaagtgcaa ttaaaccaat
     4981 ggaaaagaaa aatataaccg ataaaataga cgagcgaatg tcgttggtta aatattccat
     5041 caaaccaaaa aacagtgttc cgaagacgat accaattttt tctgccacat cgtaaaaaga
     5101 gaaatacgaa gcgtggtttt ctgttcgtgg caacatttta gaataggttg aacgtgccaa
     5161 cgcttgtgtt ccgcccatta ccaaaccaac tccagctgct aggtaataaa attcggttgg
     5221 cgtttgaatg aaatatgccc acaaacacaa aagcacccat atcgcaaccg caataattaa
     5281 cgagcgaatg ttgcccaaca cattggaaac tctcgacaag ataaaagcgc caaaagcccc
     5341 caataattga atcaacaaaa tggctaaaat caaacctgtg gttgcttggt cggcatattc
     5401 tccagaaaaa atttcaattt tagcatacaa aacagccatt agcataacgg tttgcacacc
     5461 ggtattgaaa aagaaaaacg acaataaata gcgtttcaat aatttggatt gattgaactc
     5521 aaaaaacaca ttttttaatt ctttaaatcc tctggtcaat ttattaggaa cagcagcttt
     5581 gttgtacaca ttattgggca atcttctaaa agttatttgg ctgaatgaaa cccaccaaac
     5641 tcccactaaa ataaagctaa cgcctgtata ttctggcccg attcccatta ttattgccaa
     5701 gcaaataatg agcaacaaca tactacctaa ataacccatt gtaaagccac ttgctgagat
     5761 tttatcgtgc aaacgcggca ctgcaatttc gggcaaaaat gcattgtaaa acaccaaact
     5821 gttccaaaac ccaatgctgg ctaaaaacac cgatagcatt ccaatttcaa acggcgcttt
     5881 tccaaaccag tacaaagaaa cacacgacaa tgctcctaag taacagaaaa actgtaggaa
     5941 acgtttttta gagcccgtca catcggcaat ccccgacaac atgggtgata aaatagaaac
     6001 caccaaaaaa gaaattgaca aagtaaacga catcaatacc gaaggtgaaa gttgtgtacc
     6061 aaaaaacggc actacaacat ctacattagc aggtacttct gtcaaattca agttgttggc
     6121 agcattgtaa ttttttaaaa atgctttggt ggtaacgtta tcataaaaaa taggaaatat
     6181 agcagaagat ataacaagat tgtaaactga gtttgcccag tcataaaaaa tccatcctct
     6241 tattactttt ctatctgctt gtgctctcat tcaaccgtat ttggtgtgtt tgcataatca
     6301 ctcaaatatt caaaatgaga tgtaaggttt ccattcaaat ctgtttcaga accacggaaa
     6361 ataatctgat cggggtcttc tctaaataat cgaggaaaaa cacttctaat caactcattt
     6421 ccaaaatctt cggatgcatc tttaggcaat tcacacggta aattatcaac tgccattact
     6481 gcaactgcat cttcgtccat aaaatcaact tccttagccg taaatggatt gtaaccataa
     6541 atagcatcgc caattttact tgggcgaatg gtgctcacaa taggatctgc aatatcacaa
     6601 gaaacatctg caataacttt caacttgcat tttggattgg ttaacaattc cgttgtaatt
     6661 aacttaggtg ctctgttgct ccaaaaatga caaggaatat acatggttgt atcctttaaa
     6721 atataggtat ccaacgttga tttataggtt tctggtgctg aataaaaatc tttttttaca
     6781 aactcacctc cgtttttagg tgcatagtaa tcttctgtat ccaaatgcgt ataaactggt
     6841 tcgtcgaatg tttcttccaa atattcgcgc ggactcactt ctttgattgg caataagtct
     6901 aaaatttctt ttgcaccgtt gcctacacgt ccccatcctg taacaacaat ttttgtgttt
     6961 gcgggtaaag aaacattcac caattctttt tctaattcct cgcgatgctc acacaaataa
     7021 gcgggtttta gattgaacgt ttttgttttt aatcctaatg ttaaaaaacc attataggtt
     7081 ccaacaattc cggcatagcg accaaagcca atgatgcgtt tattgttttt atcttttaga
     7141 gcttcgtaat ctattaattg aattttttta tccaaaatgg cacgcaacaa tttttggttg
     7201 tatgcctgtt ttttcatagt atgcgaaaag aacatgtatt ttttattcgg aattaagtcg
     7261 ctaatattca cttcttttac tcccaacaac acatcgcaag ctgataaatc ttcgtttaaa
     7321 gctatacctt ctgcacggta ttcttcgtct ttgtaactac gaaatggact tggttgaaca
     7381 atcacttcca cctctggata ttgcgcttct atagttttgc actgttttgg agttaacggt
     7441 acacgcaaat cgcgaggaat tttaccttct cttacaattc caattctaac ctgtttcatt
     7501 tcttaatttc gagtcaataa atataactaa attttatcaa ttaagtgtaa atcaataaat
     7561 tctcttacac atcctctccc tccattcgta ttcaaaacta tgtggcaatt gtttttcact
     7621 tcttgcacgg catctttagg acaagcggca atggatacgc ttttcatgat atccaaatcg
     7681 ttgatatcat ctccaatgat ggctacttct gctaatgata aattcaattt ttcgataatt
     7741 tgttgaagca catcgatttt tttatcacgc gaaacaacac aatgttgaat acctaacatt
     7801 tttgctcttg cctcaaccga ttgggaaaca aaaccggaag agataatggc aatttgaaaa
     7861 ttatttttgg tgagttgaat aattgccatt ccatcttttg tattgaactt tttgaattgg
     7921 tcgccacttt cagtgaaata cattcctccg tcggtcatca cgccatcaac atccaatacc
     7981 aagagcttaa ttttttttaa tttatctttt aaatgttggt tgggaaaaag gggcttgaat
     8041 agcaaggcat acaaatcaac ttcatattct tcggcaattg cttgcaaatc aaacaaggtt
     8101 aattcttgca cattttctat tccaaaatca gctaaaaaag ctacgtgatc caatccatat
     8161 ttggagcaca accctttgat attttcatcc aacatcatac actatacaat tttatatcca
     8221 acagcttgtg caactgtttg taattcattt tttaatgctg taaattcttg atgattcaat
     8281 tgctgagcag catctgattt ggcaacttta gggttgggat gtgcctcaat caacaatccg
     8341 tcaacgccca ttgctacaca cgctttggca agtggcaaca caccataagc ataaccaatg
     8401 gcatgcgatg gatccaaaat gataggtaaa ttggtgtatt cttgcaacca agcaacacca
     8461 cacaaatcta gcgtaaaac
//
LOCUS       DAOQVQ010000051         8383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9420_length_8383_cov_3.334054, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000051 DAOQVQ010000000
VERSION     DAOQVQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9420_length_8383_cov_3.334054"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKN22_02740"
     CDS             <1..648
                     /locus_tag="PKN22_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS41651.1"
                     /translation="PSIAEIIQIERNIRLLEYTESTIHLTGISSAEGVELVRQAKKRG
                     LAITADVHVANLQYNEEAVLGFDANFKFMPVLRRESDRKALWQGVLDGTIDAIVSDHR
                     PHDKEEKDVEFDHAHFGSIQLQTTFGALGNCGEFELSAVVNALSNNPRSILSLPMASI
                     EIGNKADLTLFAPQQKWTFTKEMVCSNTFNSDLIGKELTGKTIAVIHNNTYYLKN"
     gene            652..1212
                     /locus_tag="PKN22_02745"
     CDS             652..1212
                     /locus_tag="PKN22_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNS41652.1"
                     /translation="MATNQKEFRKQFWNQKKTVGAIAPSSRFLAKKMLQNIDFKNNKV
                     IIELGPGTGVFTEEIISKMAPDAQLLVFEINDAFYHGLAGKIKDSRVHIIHDSAEKIE
                     HYLQQYNLDKADVVVSSLPLAVFTPELRTGIISASKSALHPHGKYIQFQYSLQAKSLL
                     KKFYKRVNISFTPFNFPPAFVYTCIK"
     gene            1362..1913
                     /locus_tag="PKN22_02750"
     CDS             1362..1913
                     /locus_tag="PKN22_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41653.1"
                     /translation="MMVLLVFACKNEKSNDVSVSELEDYYEFQGFNLTPFDINATIML
                     PDETANIGASTQPDVEHIQSDFYWQINIGQNFHLYIEDYGDNTNLVKAHKEKLKNTSF
                     YDVEYVLEEPELIIYKVKLKVRGNQNASKRVGINHEAYHVYAEKVVNGIHYELRSSDE
                     GASKEIIELMAKSIRSFKGKNDK"
     gene            complement(1915..2322)
                     /locus_tag="PKN22_02755"
     CDS             complement(1915..2322)
                     /locus_tag="PKN22_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNS41654.1"
                     /translation="MKSPQEIVQLMMNNDAFSQWMGVEVEEISLGSCQLKMTLNATML
                     NGFYIAHGGISYSLSDSALAFAANSYGYKCVSVETSISHTRPAKEGSVLRAKAIEKHR
                     GKTIAIYEVVVTDENEKTVALFKGTVHISQEIW"
     gene            complement(2328..2807)
                     /locus_tag="PKN22_02760"
     CDS             complement(2328..2807)
                     /locus_tag="PKN22_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41655.1"
                     /translation="MKQVTYESIEKAINIIDNLPDDQLETIASDFSKAQPTLISYALS
                     ASEEFNNERLQGLLIYYFCLIIEAFRQEGIELNEVKEEEVDEFEEPFFQALDTYFQTE
                     NIETLEEFVGQSVLVQFMTIEISTPDEDGTALDDETATQLFIVTLAVIYLLTNSIKG"
     gene            complement(2814..3410)
                     /gene="coaE"
                     /locus_tag="PKN22_02765"
     CDS             complement(2814..3410)
                     /gene="coaE"
                     /locus_tag="PKN22_02765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNS41656.1"
                     /translation="MAFKVGITGGIGSGKSMICQLLEEKGFPIFYSDDESKKLVQTHP
                     EIKKELIALLGQNVYVNNQLNKTFLAAQLFNDDHIREKVNQIIHPVVRKTFAAWAENQ
                     TSPIVFNEAAILIETGTYKTLDFTVLVVAPKEVKIQRVMERDGVSEAEVEARMNKQWS
                     DEQKIPLADFIIHNDGSALNHQLENLLEKINSLALQTK"
     gene            complement(3410..3823)
                     /locus_tag="PKN22_02770"
     CDS             complement(3410..3823)
                     /locus_tag="PKN22_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNS41657.1"
                     /translation="MNFKTNTMESIDKYCVIGTPKTPEIDFNRESGVMLFKGRSIPEN
                     SLDFFQPIYDWIDKYGDHPQPNTVVQIKLEYFNTSSSKCILDMLRKFETINNGKSKVR
                     VEWYFETDDEDMEEAGEDYQAIVELPFKMIEVEEI"
     gene            complement(4373..>4774)
                     /locus_tag="PKN22_02775"
     CDS             complement(4373..>4774)
                     /locus_tag="PKN22_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNS41658.1"
                     /translation="LYSSMRTSDDLTVESSYFHAELTRLRFIMDAIEQKGNVFVILDE
                     ILKGTNSKDKEMGSAEFLKKLNRVGAKGIIATHDLSLTDLAKTTPAFRNVYFDSTITN
                     DDLYFDYKMRNGVCQNMNASFLLKKMQLIDG"
     assembly_gap    4775..4874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4875..6345)
                     /locus_tag="PKN22_02780"
     CDS             complement(<4875..6345)
                     /locus_tag="PKN22_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41659.1"
                     /translation="MTLFLLETVNLLSIIQFMQEQLSFYTQQIEVHSALLEQAKKKLR
                     YFSTLRLLAFVILVLSCYFIGFQAVLLPVLIVEITVFIYLINKWLDAKIIKEKEELYI
                     QLNQQEIKLLGGNWSSFDNGEEFKSAQHPFANDMDLFGKKSVYQYVNRTVLPLGAKML
                     AQTLGEGAKNKVLNQQMIAELSKNIEWCQRFIVESKVYLKNEERQQSLDALHELTVGQ
                     ARLLFLRWILPVVSITSLVIYNLDVLSFEQLLLVIVGVLGVIGINLKHTNHLMHTLSN
                     RSEKINALINQLEQLKTIQLQTKEGNELKAQLFDKTGVYEGLKALERIKKRSEYRMNT
                     VVGVVLNFLFAWDFHLLAQTQKWKQQFNQSMAHVETDLAQVEVWISGAIYQFNSQQTC
                     FATFSTNNDFKISALRHPFIPFDKQIANDFDLSNDESFFIITGPNMAGKSTYLRSVGW
                     AIISANAGFPILANSCILPEMQLYSSMRTSDDLTVESSYF"
     gene            6416..7309
                     /locus_tag="PKN22_02785"
     CDS             6416..7309
                     /locus_tag="PKN22_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41660.1"
                     /translation="MKTTYKFTLVCGILALTVACTSNTANTDKEHITVDQENVDKTLN
                     TRFNGRIFSVPSPILTMTLLRKIEPTFKASLLNNVSKVDSYQTEYKRALNLGIYGADL
                     GYSSIYMEKKQIMDYIVTVEKITKSLGLESAFDKSFFERYQKHTDNTDSMMHIVSEAF
                     KNADLFLKNDNRKTVSSLILAGGWVESMHFATSLMKNNKNDKELIDRVGEQKQTLQTL
                     IAVLEENNKNNESDQLIQSFRDLKTSFDQILMNYKYAEPTTDAKKHITTLNHDISFDV
                     QSATLNEITEKITKLRTHIIN"
     gene            7314..7745
                     /locus_tag="PKN22_02790"
     CDS             7314..7745
                     /locus_tag="PKN22_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41661.1"
                     /translation="MKSTIFFFAILCSMFGFYSFGQSCDELIKECEKLFLNEKGKAEF
                     VSDGQVYNAFLDRQQAEFEVTFYGGTTYRIAVSAGSEDDYVLFKLRDPNGNVLFSNQD
                     YKNARYWDFKIPKTINLKIETSLDSERKISGCSVMLIGFKQ"
     gene            7758..>8383
                     /locus_tag="PKN22_02795"
     CDS             7758..>8383
                     /locus_tag="PKN22_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41662.1"
                     /translation="MSERILKALMQLFAIIARVDANNENSEELTISAHQIIRSFLKVE
                     LSTNKIDQYLELFDKHFDELRLKKKKQEGEINKRTSVNSVKVLRICSDINKELTNNQR
                     FIVLVRMMELVLQNGIPTKLEEEFLITISEVFKIDEKEYKLIYSLLQNQINTNDLPDR
                     CFVITGKKSDKPQHIFLKNLDKKIICIHIKSIDYIFFKYLGNQNLYLNG"
BASE COUNT         2760 a         1500 c         1375 g         2648 t
ORIGIN      
        1 ccatctattg cagaaataat ccaaattgag cgtaacattc gtttgttgga atataccgag
       61 agtactattc acttaaccgg aatttcaagt gccgaaggag tggaattagt tcgtcaagca
      121 aaaaaacgtg gattagcaat tacggccgat gtgcatgttg ctaatttgca atacaacgaa
      181 gaagcagtgc ttggatttga tgctaatttt aaattcatgc cggtattgcg ccgtgaatcg
      241 gatagaaaag ctttatggca aggtgttttg gacggaacca ttgatgcaat tgtatccgat
      301 caccgcccac acgataaaga agaaaaagat gtagaatttg atcatgccca ttttggtagc
      361 atccaattgc aaacaacctt tggagccttg gggaattgtg gagaatttga actatcggca
      421 gttgtaaacg ctttgagcaa taatccacgc tctattttat cccttccaat ggcttccatt
      481 gaaattggca acaaagcaga tttaacgttg tttgcgccgc aacaaaagtg gacatttacc
      541 aaagaaatgg tttgttccaa cacctttaat tccgacttaa ttggcaaaga attaactgga
      601 aaaacaattg ccgttatcca caacaataca tactatttaa aaaactaatc aatggctaca
      661 aaccaaaaag aatttagaaa acagttctgg aatcaaaaga aaacagtggg ggcaattgct
      721 cctagttctc gctttttagc aaaaaaaatg ttgcagaata ttgattttaa aaacaataag
      781 gtcattattg aattagggcc tggaacagga gttttcaccg aagaaattat ttctaaaatg
      841 gcgcccgacg ctcaattgtt ggtatttgaa ataaacgacg ctttttacca cggtttagct
      901 ggaaaaatta aagatagtcg cgtgcatata attcacgatt ctgccgaaaa aattgaacat
      961 tatcttcaac aatacaattt agacaaagct gatgtagttg tttcatcgtt gccacttgcg
     1021 gtttttactc ccgaattgag aacgggcatt atttctgcct ccaaatcagc cctacatcca
     1081 catggaaaat acatccaatt ccaatacagt ttacaagcaa aatcattgtt gaagaaattt
     1141 tataaacgcg ttaatatttc atttacaccg tttaattttc cacccgcttt tgtatacaca
     1201 tgcattaaat aattgggtta aaactaaaaa atagtcttaa aaattaggtt aattcatcat
     1261 tttttgtaaa tttgcttgga ctataaaact tgaaaatgaa tttttttacc tattttactg
     1321 agagacctaa cacacattta caaaagctag ctgtcatttt tttgatggta cttttagttt
     1381 ttgcttgtaa aaatgaaaaa agtaatgacg ttagtgtaag tgagttggaa gattactatg
     1441 aatttcaagg ttttaattta actccatttg atattaatgc aacgattatg ttgcccgatg
     1501 aaacggcaaa cattggtgca tctactcaac ctgatgtaga acacattcaa agcgattttt
     1561 attggcaaat caacataggg caaaattttc atttgtacat cgaagattat ggcgataaca
     1621 ccaatttggt gaaagcgcat aaagaaaaat taaaaaacac cagcttttat gatgtagaat
     1681 acgttttaga agaaccagaa ttaattattt acaaagtaaa actgaaagtt agaggtaatc
     1741 aaaatgcttc taaacgtgtg ggtatcaacc acgaagctta tcatgtgtat gctgaaaaag
     1801 ttgtgaacgg cattcattat gaacttagaa gtagcgacga aggagcatct aaagaaatca
     1861 ttgaattgat ggcaaaatca atccgatctt ttaaaggtaa aaacgataaa taaattacca
     1921 aatttcttga gaaatatgta cagtaccttt aaataaggct actgtttttt cattttcatc
     1981 ggtcaccacc acctcgtata tagcaattgt ttttccacgg tgtttctcaa tagctttggc
     2041 gcgtaaaaca cttccttctt tagccggacg tgtatgcgaa atagatgttt caacactcac
     2101 acatttgtaa ccataagaat tggctgcaaa cgccaacgca gaatcggaca acgaataact
     2161 aataccacca tgtgcaatgt aaaatccatt gagcatagtg gcgtttaaag tcatttttaa
     2221 ttggcaagaa cccaacgaaa tttcttccac ctccactccc atccattgac tgaaagcatc
     2281 gttgttcatc attaactgaa caatttcttg aggtgatttc atgaatttta tccttttatt
     2341 gaattggtta acaagtaaat aaccgctaaa gttacaataa acaattgagt ggctgtttca
     2401 tcgtccaatg ccgtgccgtc ttcgtcgggt gtagaaatct caatggtcat aaactgcacc
     2461 aacaccgatt gtccaacaaa ttcttctaac gtttcaatgt tttctgtttg gaaataagta
     2521 tctaacgctt ggaaaaaagg ctcttcaaac tcatccacct cttcttcctt cacttcgttt
     2581 aattcaattc cttcttgacg aaaagcctca ataatcaaac aaaaataata gattaacaag
     2641 ccttgcaaac gttcattatt aaattcttca gaagccgaca atgcatagct gattaacgtt
     2701 ggttgcgctt ttgaaaaatc cgatgcaatt gtttctaatt ggtcgtctgg taaattatca
     2761 ataatgttga tagctttttc tatactttcg tatgtaactt gtttcatgtt ctattatttt
     2821 gtttgcaaag ctaatgaatt tattttctcc aataaatttt caagctgatg atttaatgca
     2881 cttccatcgt tgtgaatgat aaaatcggct aaaggtattt tttgttcatc cgaccattgt
     2941 ttgttcatgc gtgcctccac ctctgcttca gacacaccat cgcgctccat aacacgctga
     3001 atcttcactt cttttggcgc caccaccaac accgtaaaat ccaaggtttt ataagtgcct
     3061 gtttctatca atatagccgc ttcgttaaaa actatgggac ttgtttgatt ttctgcccaa
     3121 gcggcaaagg ttttgcgcac cactggatgg atgatttgat tgactttttc acgaatgtga
     3181 tcgtcgttaa acaattgagc cgccaaaaaa gttttattaa gttggttgtt aacatacacg
     3241 ttctgaccaa gtaacgctat gagttctttt ttaatttcgg ggtgtgtttg cactaatttt
     3301 tttgattcat catccgaata aaaaatggga aaacctttct cctctaacag ctgacaaatc
     3361 attgatttac cacttccaat cccacctgta attccaactt taaatgccat tatatttcct
     3421 ctacttctat cattttgaaa gggagttcaa caattgcttg atagtcctca cctgcttctt
     3481 ccatatcttc atcatccgtt tcaaaatacc actcaaccct cacttttgat ttaccattat
     3541 tgattgtttc aaactttctc aacatgtcta aaatgcattt tgaagaagag gtgttaaaat
     3601 actccagctt aatttgcact accgtatttg gttggggatg atcgccatat ttatcaatcc
     3661 agtcgtaaat aggttgaaaa aaatctagtg aattttctgg tattgatcgc cctttaaaaa
     3721 gcataactcc agattccctg ttaaaatcaa tctcgggtgt ttttggagtg cctataacgc
     3781 aatatttatc aatactttcc atggtgttag ttttaaaatt cattatttcc agtgtaaaag
     3841 tgtttgaacg aatatacatt tattttttta ttaaaaaaat ttttttttct ggataaatcg
     3901 tataagtagt gtagttgtct gtgtttttga ggtggaaaaa attggtattt tgttgttgag
     3961 gaatgggtaa aaatactcct ttataattga ttagtaaagg tgtcagtgaa acattcaacc
     4021 aacgaatgaa ataaaaaagg cgagttgccc cgccttgaat gttttcacaa cggacttctg
     4081 ttgtttatga ccgtgaattt cataaaatga ataagaacta aaaggtaggg tacttttttt
     4141 tcaaccaata aggttactaa aacttcacaa agaccacaat gtttttttct ctgcgttctc
     4201 tatggtaaaa aaataaccgc aatggccgct atggtttacg caatgtgcgc aatgtttttc
     4261 tctgcgatcc ctgcggattc ttggcgtaac tccgcggtta agtttcaacc gcaatggtcg
     4321 caaagtattt tataattcat taaaaacacg attaaacgat tgaatcaaca aattacccat
     4381 caatcaattg catttttttc aataaaaacg atgcgttcat gttttggcat acgccattac
     4441 gcattttgta gtcgaaatac aaatcatcgt tggtaatggt tgaatcaaaa taaacgtttc
     4501 taaacgctgg agttgttttt gctaaatcgg tgagcgataa atcgtgggtg gcaatgatac
     4561 ctttggctcc tactcgattc agttttttca aaaattcggc cgaacccatt tctttgtcct
     4621 tgctgttggt accttttaaa atctcgtcca aaatcacaaa cacatttcct ttttgttcaa
     4681 tggcgtccat gataaaacgc aaacgggtta attcggcatg aaaatacgaa ctttcaacgg
     4741 ttaaatcatc actggtgcgc atgctagaat acaannnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnngaaaat acgaactttc aacggttaaa tcatcactgg tgcgcatgct
     4921 agaatacaac tgcatttcgg gtaatataca cgaattagct aaaattggaa aacctgcatt
     4981 ggccgagatt attgcccatc caacactgcg taaataggta cttttacccg ccatgttggg
     5041 acctgtgatg atgaaaaacg actcatcgtt ggataaatca aaatcgttgg ctatctgctt
     5101 atcaaaaggg atgaatgggt gtcggagtgc cgaaatttta aaatcgttgt tggtgctaaa
     5161 ggttgcaaaa caggtttgtt gactgttgaa ttgataaata gctcccgaaa tccacacttc
     5221 aacttgcgcc aaatctgttt ctacgtgcgc catagattgg ttgaattgtt gcttccattt
     5281 ttgggtttgt gccaataaat gaaaatccca ggcaaataaa aagttcaaca caacacctac
     5341 aaccgtgttc atgcggtatt cggagcgttt tttgatgcgt tctaacgctt ttaaaccttc
     5401 ataaactccc gttttgtcaa acaattgcgc tttcaattca tttccttctt tggtttgcaa
     5461 ttgaatggtt tttaactgtt ccaactgatt aatcaacgca tttatttttt cagaacgatt
     5521 gctcaacgta tgcatcaaat ggttagtgtg ctttaagttg atgccgataa cacctaaaac
     5581 acctacaatt actaacaata actgttcaaa cgataataca tccaaattgt atataaccaa
     5641 cgaagtgatg gaaacaacgg gcaatatcca tcttaaaaac aacaaacgcg cttgaccaac
     5701 cgtaagttcg tgcaaagcat ccaatgattg ttggcgttct tcatttttta aatacacttt
     5761 agattcaaca ataaatcgtt ggcaccattc aatatttttt gataattcgg caatcatttg
     5821 ttggttcaat actttatttt ttgctccctc acccaaggtt tgcgccaaca tttttgcacc
     5881 caagggcaaa accgtacgat tgacgtattg ataaacactt tttttaccaa ataaatccat
     5941 gtcgttggca aagggatgtt gagcagattt gaattcttcg ccgttatcaa aactcgacca
     6001 attgcctccc aataatttaa tttcttgttg attaagctga atgtataact cttctttttc
     6061 tttaattatt tttgcatcca accatttgtt tatcaaataa ataaaaacag ttatttcaac
     6121 tatcaataca ggtaataaaa ctgcttgaaa gccgataaaa tagcacgaca aaacaaggat
     6181 aacaaacgcc aaaagacgca aggtagaaaa atagcgtaat ttcttttttg cttgttccaa
     6241 caaagcagaa tgtacttcga tttgttgagt ataaaatgac aattgttctt gcataaactg
     6301 aatgattgaa agcaaattta cggtttccaa cagaaacaag gtcattgttc ataaaaatta
     6361 ttgtgtggca taaaaaataa ttgtatatta gccatagaat tgaaaaaaat tcaacatgaa
     6421 aacaacttac aaattcacgc tagtatgtgg gattttggcg ttaacagtcg cttgcacatc
     6481 caacactgct aatacagata aagaacacat aactgttgac caagagaatg ttgataaaac
     6541 ccttaacaca cgttttaatg gaagaatttt cagcgttcca tcacctattt taaccatgac
     6601 cttgttacgt aaaatagaac caacctttaa agcttcactt ttaaacaatg tttccaaagt
     6661 tgattcgtat caaaccgaat acaaacgtgc tttaaactta ggaatttatg gtgctgattt
     6721 aggttattca tccatctata tggagaaaaa acaaatcatg gattacattg taacagttga
     6781 gaaaatcacc aagtcattag gtttagaatc tgcttttgac aaatcatttt ttgaacgtta
     6841 tcaaaaacac accgacaata ccgattcgat gatgcacatt gtgtcagaag cctttaaaaa
     6901 tgcggattta tttttaaaga atgataatcg caaaactgtt tcatcgttga tactagctgg
     6961 tggttgggtt gaatccatgc attttgctac atcgctgatg aaaaacaata aaaatgataa
     7021 agaattgatt gaccgtgttg gtgaacaaaa acaaaccttg caaacgttaa ttgctgtttt
     7081 ggaagaaaac aataaaaaca atgaaagtga tcaactcatc caatcattta gagatttaaa
     7141 aacatctttt gatcaaatat tgatgaatta caaatatgca gagcctacaa cggatgcaaa
     7201 aaaacacatc actacattaa atcatgacat ttcatttgat gttcaatcag caacattgaa
     7261 tgaaattacc gaaaaaatta caaaactacg aacccatata atcaattaag cgtatgaaat
     7321 caaccatatt tttctttgct attctctgct cgatgttcgg gttttattct tttggccaaa
     7381 gttgcgatga acttataaaa gaatgtgaaa agcttttttt aaatgaaaaa ggcaaggctg
     7441 aatttgtttc tgacggacaa gtttataacg cttttttaga tcgtcagcaa gctgaatttg
     7501 aagtaacttt ctacggaggc accacctacc gaattgctgt tagtgctggt agtgaagacg
     7561 attatgttct ttttaaattg cgcgatccaa atggcaatgt tttatttagc aatcaagact
     7621 acaaaaatgc acggtattgg gattttaaaa tacctaaaac tattaattta aaaattgaaa
     7681 cttctttaga ctcagaacga aaaatttctg gttgctcggt aatgcttatt gggtttaaac
     7741 aataatttag caattcaatg agcgaacgaa ttcttaaagc cttaatgcag ctatttgcaa
     7801 taattgcgcg agtagatgct aacaacgaaa actcagaaga actgactatt tctgctcacc
     7861 aaattattcg ttccttttta aaagttgaac tttcaacgaa caaaattgat caatacttag
     7921 agttatttga caaacatttc gatgaactcc gattaaaaaa gaaaaaacaa gaaggtgaaa
     7981 taaacaaaag aacgtcggta aattctgtta aagtgttacg tatttgcagt gacatcaata
     8041 aggaattaac aaacaaccaa cgcttcattg tacttgtacg aatgatggag ttggttttac
     8101 aaaacggaat cccaacaaaa ttagaagaag aatttcttat tactatttcg gaagttttta
     8161 aaattgacga aaaagaatac aagctcattt attctttact gcaaaatcaa ataaacacca
     8221 acgatcttcc tgatagatgt tttgtgatta ctggtaagaa atctgataag ccacagcata
     8281 tcttccttaa aaatttagat aaaaaaataa tttgtatcca tattaaaagc attgattaca
     8341 tattttttaa ataccttgga aatcaaaacc tttacctcaa tgg
//
LOCUS       DAOQVQ010000052         8117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9800_length_8117_cov_3.577648, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000052 DAOQVQ010000000
VERSION     DAOQVQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9800_length_8117_cov_3.577648"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1454
                     /gene="putP"
                     /locus_tag="PKN22_02800"
     CDS             21..1454
                     /gene="putP"
                     /locus_tag="PKN22_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HNS41663.1"
                     /translation="MAIALYMLLMLAIGVYAYRKSTSNSEEFLIGGRKLGPAVTALSA
                     GAADMSGWLLMGLPGAMYIAGMSSSWIIIGLVIGAYLNYLIVAPRLRIYTEKAQNAIT
                     LPVFFENRYADKSQLLKITSSLFILIFFTLYTSSGMVSGGKLFQSAFGFDYSVGLWVT
                     GLVVVCYTFLGGFLAVSLTDFVQGTIMVLALIVVPIVAISTIGGWNETWDLVEQKGEN
                     YLNLFSGTTTISIVSLLAWGLGYFGQPHILVRFMAIDKVENIKKARRIGMVWMIFTVT
                     GAALVGLIGIAFLQKFDTSTMAQMDASKAAAETIFIYFSRVLFHPLIAGFLLSAILAA
                     VMSTISSQLLVTSSSLTEDIYKTFLHQQATPKQLLIASRISVLLVALIAMLLALTPKD
                     TILNLVGNAWAGFGAAFGPLVLIALLHKKSTWQGALAGMLVGGITVLAWVYLPHDYKA
                     VYEIIPGFILALTTNLLVSKITYKKGQ"
     gene            complement(1586..2254)
                     /gene="trmB"
                     /locus_tag="PKN22_02805"
     CDS             complement(1586..2254)
                     /gene="trmB"
                     /locus_tag="PKN22_02805"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNS41664.1"
                     /translation="MGKDKLRRFAAVKEFTNFFEPEIPNEFYMKGKWKTDYFKNDHPI
                     VLELGCGKGEYSVGLAKHYPNKNFIGVDIKGARMYIGAVEAQEANLQNVAFCRTKIDF
                     INNCFAENEVDEIWLTFSDPQPKKPNKRLTSLPFINRYRKLLKPGGVVHLKTDSDLLF
                     EYTEEQIKENNYECLELTWDLYGELPEHLDATTKDILHIKTHYEKLFTAKGAVIKYCR
                     FKIS"
     gene            2561..2986
                     /locus_tag="PKN22_02810"
     CDS             2561..2986
                     /locus_tag="PKN22_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41665.1"
                     /translation="MQLQRIRVTIIILLFSGTITFGQDIYRLKVLDSTSFSKYNYFEF
                     KKGKDIYKVISKKKDTSSLANLCLLNLEIKNYYKLNLEQILFLEDKEDGIGVFYYDVE
                     RMIFRGKNRAFGGPEKPVYLCDSIFKNQISCECLVDQFW"
     gene            2958..3158
                     /locus_tag="PKN22_02815"
     CDS             2958..3158
                     /locus_tag="PKN22_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41666.1"
                     /translation="MSAWLINFGRVLAQKDISKGYMNRINPEFKPDAPLLKVTDANKI
                     ITIYIKGKEATLMELYEFNKKL"
     gene            3161..3934
                     /locus_tag="PKN22_02820"
     CDS             3161..3934
                     /locus_tag="PKN22_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41667.1"
                     /translation="MKYLILYFTFFISTLISCSQTYKIYDKKRSAFDLLQIKGEVNSV
                     VEYTCSATDSLGKIKLKDTLQIPTPKEWVFDSTGSIKFQNLLNYKNLNNQISHKFIIY
                     DHNKMKELWYEVRENDTLLVEKMQLNDSLKNIHSISYNYAGTVTSKSSTKYTSKKVET
                     TITNKYSKDQTKTIGFLNENGKVDSLKVFINGKLHTEIRNEYQNNELSKIVSKNADGL
                     EKELLFKYNYDDLGNWVEKIMYENGNLISVTIRKINYNR"
     gene            4048..5175
                     /locus_tag="PKN22_02825"
     CDS             4048..5175
                     /locus_tag="PKN22_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41668.1"
                     /translation="MRVATKKYKQINSWKEICFTITDATTIATDNQTVAGNYSLSFIP
                     NTSTATVHFGANSTVALHTIHISYPHIDTLITQPRKGGYRYGFQNQEHDDEVKGVGNS
                     VNYKYRMHDPRLGRFFAVDPLAGKYPFYSPYAFSGNRVLDAIELEGLEPVFIHGTWSN
                     KETFNHSFVKGMVNATGWDESKVAYLSWSGSNSVEARQEAATILIEYLTSDENMAYLE
                     SKGQAMHATLIAHSHGGNVGKLVKEALEEQGWTVDLINISTPQRDDFQTSNKGGGKYL
                     NFYSNVDLIQYIGSINSFESFITESRIDSNADKNIEVDNWHRNPYLELGKNIPGIQGA
                     SSFIDGVVNAFKWVFSDGAGHSVHNSDAQDHIIKETENEFK"
     gene            5694..6152
                     /locus_tag="PKN22_02830"
     CDS             5694..6152
                     /locus_tag="PKN22_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41669.1"
                     /translation="MHLIDTKTNKRYEVVVDIVDEQDFKQITKSKFFFNWKEEKKNII
                     YKLMLDDEILGLMSCFDNEGDKRIEIKLLSVSKENRGQNKRYERIAGTLIGYACREAT
                     KRYGIEGCVSLVPKTQLKHHYIQQYGMMDAGKQVFLEGEQLLRMLKEYEL"
     gene            6149..6670
                     /locus_tag="PKN22_02835"
     CDS             6149..6670
                     /locus_tag="PKN22_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41670.1"
                     /translation="MKNRKLNEKYTDKELAESFVFRDDLSAEEKKQSDADLREIRNNL
                     RSKVTPKQMLLSRLLQLKYQIEDYLKNPNYDAERSFGFFLRSYLDALEIKHKDFASDI
                     SINETELSQILNKHRNPSEKIIIRLEIHSNKIIPAITWFRLLEKQREYEIMTDNSLRI
                     RERDRVSNILKVS"
     gene            7163..7549
                     /locus_tag="PKN22_02840"
     CDS             7163..7549
                     /locus_tag="PKN22_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNS41671.1"
                     /translation="MAKNKKIEVEGKEITIILDKEQEYISLTDMLKAKDGDFFISDWL
                     RNRNTVEYLGIWESVYNPTFNYGEFATIKSQAGLNKNRPNIQSGEIAERLAIPAPTVK
                     RILSELLNKGLIEKQGNGRNVSYTIK"
     gene            7717..>8117
                     /locus_tag="PKN22_02845"
     CDS             7717..>8117
                     /locus_tag="PKN22_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS41672.1"
                     /translation="MNATASKSEQSTNYSALSTLTTVFFFWGFIASGNSVFIPFCKEY
                     FGLDQFQSQLIDFAFYGAYYLGALALFLISSKRNKDLVGTWGYKKTIVIGLLFSAIGA
                     GAMIVSVFSNVFSGMLLGLFIVALGFSLQQTS"
BASE COUNT         2848 a         1265 c         1460 g         2544 t
ORIGIN      
        1 tgcaaacaac atacgaaata attgccattg cgctttatat gttactcatg cttgccatag
       61 gtgtttatgc ctaccgcaaa tcaacgagta attcggaaga atttttaatt ggcggaagaa
      121 aattaggacc tgccgttaca gcactttctg caggcgcagc ggatatgagt ggttggttgc
      181 tcatgggatt gccgggcgcc atgtacatcg caggcatgtc gagcagttgg atcatcattg
      241 gtttggtgat aggtgcctac ttgaactacc ttattgttgc cccacgcttg cgcatttata
      301 ccgaaaaagc tcaaaatgcc attacactcc ccgtgttttt tgaaaacagg tatgctgata
      361 aaagtcagct gttaaaaata acatcctcgt tgtttatttt aatctttttt acgttgtaca
      421 cctcatcggg catggtgtcg ggtggaaaat tgtttcaatc ggcatttggg tttgattatt
      481 ctgttggctt gtgggttacc ggactagtgg tggtttgcta cacgtttttg ggtggttttt
      541 tagcagttag tttgaccgat tttgtacaag gaaccatcat ggtgctcgct ttgattgttg
      601 ttccaattgt tgccatttca accataggcg gatggaatga aacttgggat ttggtggaac
      661 aaaaaggtga aaactacctc aatttattca gtggcacaac aaccatcagc attgtttctt
      721 tgcttgcttg ggggttggga tattttggac aacctcatat tttggtgcgt tttatggcaa
      781 ttgataaggt tgaaaacatc aaaaaagcaa gaagaattgg aatggtttgg atgattttta
      841 ccgttacagg tgccgcccta gtaggattaa ttggcattgc ttttctgcaa aaatttgaca
      901 cctccaccat ggcacaaatg gatgcttcga aagctgctgc agaaactatt tttatctatt
      961 tctcacgtgt gttgtttcat ccattgattg caggattttt gctttcggcc atattggctg
     1021 cggtaatgag caccatttca tcgcaattat tggtcacttc aagttcatta accgaagata
     1081 tttacaaaac ctttttgcac cagcaagcca ctccaaaaca attgttgatt gccagcagaa
     1141 tttcggtgtt actagttgcc cttattgcaa tgttgttggc actaactccc aaagacacca
     1201 ttttgaattt agtgggcaat gcgtgggctg gttttggcgc agcgtttggt ccactggttt
     1261 taattgcatt gttgcacaaa aaatctacgt ggcaaggagc attggccggc atgttggtgg
     1321 gtggcatcac tgttttggcg tgggtgtacc ttcctcatga ctacaaagct gtttatgaaa
     1381 ttattcctgg gtttatttta gcgttgacaa ccaatcttct tgtttcaaaa attacttata
     1441 aaaagggaca atgagcaaaa gcatcttttc cattttagca gttaaaaatc gaaaggaaaa
     1501 aatagataaa accatcaaaa ttgcttatct ttcggattca actcaaacaa taaaagaata
     1561 ttaacaagaa tatttcctaa aaaaattaag aaattttaaa acgacagtat ttgattaccg
     1621 caccttttgc tgtaaaaagc ttttcgtaat gggttttgat gtgcaaaata tctttggtag
     1681 ttgcgtccaa atgttcgggc aattcaccat acaaatccca cgtaagttcc aaacattcgt
     1741 agttgttttc tttaatttgc tcttcggtat attcaaatag caaatcacta tcggttttaa
     1801 ggtgcaccac gcctcctggc ttcaataatt tgcggtaacg attgataaat ggaagtgaag
     1861 ttaagcgttt gttgggcttt ttaggttgtg gatcagaaaa agttaaccaa atttcatcca
     1921 cttcattttc tgcaaagcaa ttgttgataa aatcaatttt tgtacgacaa aaagcaacgt
     1981 tttgcaggtt ggcttcttga gcttctactg ccccaatgta catgcgtgcg cccttgatat
     2041 ccacccctat aaaatttttg ttgggataat gttttgccaa tcctaccgaa tattcgcctt
     2101 taccgcatcc taattcaagc acaattggat gatcgttttt aaaataatcc gttttccact
     2161 ttcctttcat gtaaaattcg tttggaattt ctggttcaaa aaagttggta aactctttta
     2221 cggcagcaaa acggcgcaat ttatctttcc ccataatttt atcgctcaac taaaaataaa
     2281 caccttttca atttggtggc gcaaaaatag ctaaaattcc aacaaggaag gcgaagttta
     2341 caaaaatccg atgttgctgt tttttggata caattgcaca aaaggtggtg taattcactt
     2401 tacttcaaac caagagctgc tttaaaaatt gaaagaggaa agtaaaaaat aataaaagaa
     2461 tcttgccatc taaaaagaaa aatgtaactt tcgtgttcaa ttgttttatg acgactggaa
     2521 ctaaagaaaa acctatttct ataccaaaag ataacattat atgcaattac aaagaatcag
     2581 agtaaccata ataatattat tattttctgg tactataact tttggacaag atatttatcg
     2641 tttaaaggtt ttagatagta ctagtttttc taagtataat tactttgagt ttaaaaaagg
     2701 aaaagatatt tataaagtta tatctaaaaa aaaggatact agctctttag ctaatttatg
     2761 tttgctcaat ttagaaatta aaaattatta taaattaaat ttagaacaaa ttctatttct
     2821 tgaagataaa gaagatggca taggtgtgtt ttattatgac gttgaaagaa tgatatttag
     2881 aggtaaaaat agagcgtttg gaggacctga aaaacctgtt tatttatgcg attctatttt
     2941 taaaaatcaa atcagttgtg agtgcttggt tgatcaattt tggtagagtt ttagctcaaa
     3001 aagatatttc taaaggttat atgaatcgaa ttaatccaga atttaaaccc gatgcgcctt
     3061 tattaaaggt tactgatgcc aataaaatta ttaccatata cataaaagga aaagaagcaa
     3121 ccttaatgga actgtatgaa ttcaataaaa aattatgatt atgaaatatc taattcttta
     3181 tttcactttt tttatttcaa ctttaattag ttgttctcaa acatataaaa tttatgataa
     3241 aaagcggagt gcatttgatt tattacaaat aaaaggtgaa gtaaattcag tggttgagta
     3301 tacgtgttca gcaaccgata gtttgggtaa aattaaatta aaagatactc ttcaaatccc
     3361 cactcccaaa gaatgggtat ttgactctac tggaagtata aaattccaaa atctactcaa
     3421 ttataaaaat ctaaataatc aaatttctca taagtttatc atttatgacc ataataaaat
     3481 gaaggaactc tggtatgaag taagggaaaa cgatacgctt ttggtggaga aaatgcaact
     3541 aaacgattct ttaaaaaaca ttcattctat tagttataat tatgctggaa ctgtaacaag
     3601 taaatcaagt acaaaatata cttcaaaaaa agtagaaaca accataacca ataaatactc
     3661 aaaagaccaa actaaaacaa ttggcttttt aaatgaaaat ggtaaagtag attcactcaa
     3721 agttttcatt aatggaaaac tacacacaga aataaggaat gaatatcaaa ataacgaatt
     3781 atctaaaatt gtaagtaaaa atgctgatgg gttagaaaaa gaacttttat ttaagtacaa
     3841 ttatgatgat ttaggcaatt gggttgaaaa aataatgtat gaaaacggta atttgatttc
     3901 agtaacaata agaaaaataa actacaatcg gtaatatttc actatcttta aaatttctaa
     3961 tggcagtgat taaacttaaa acagaatccc caaaacactc cttatctcaa aaaaacacta
     4021 actttaggct aagaacagca attgcttatg agagtggcaa ctaaaaaata caaacaaata
     4081 aacagttgga aggaaatttg tttcaccatc accgatgcca ccaccattgc aacggataac
     4141 caaactgttg cgggcaatta cagcctttcg tttattccca acacatccac agcaaccgtt
     4201 cattttggcg ccaactcaac ggtggcactc cacaccatcc atatcagcta cccccatata
     4261 gacaccctca tcacgcaacc tcgcaaaggc ggatataggt atggcttcca aaatcaagaa
     4321 cacgatgatg aagtgaaagg tgtaggaaat agtgtaaatt acaaataccg catgcacgac
     4381 ccaagactag gaagattctt tgcggtggat ccgttggcag gaaaatatcc tttttattct
     4441 ccttatgcat tttctgggaa tagagttctc gatgcaattg aattagaagg cttagaacct
     4501 gtatttatac atgggacatg gagtaataaa gaaacattta accacagttt cgttaaagga
     4561 atggtaaatg caactggatg ggatgaatcg aaagttgcat atttgagttg gtcaggaagt
     4621 aatagtgttg aagctagaca agaagcagca accatactaa ttgaatattt aacttctgat
     4681 gaaaacatgg cataccttga atctaaaggt caagctatgc acgcgacatt aattgctcat
     4741 agtcatggtg ggaatgttgg aaaattagta aaagaagcat tggaagaaca aggatggaca
     4801 gttgatttaa taaatatatc cactccacaa agagacgatt ttcaaacaag taataaaggt
     4861 ggtggaaaat atttgaattt ttatagtaat gttgacttaa ttcaatacat aggttcaatt
     4921 aatagttttg agtcattcat tacagaatca aggatagact ctaatgccga taagaacata
     4981 gaggttgaca attggcatcg aaatccctac cttgaacttg gtaagaatat tcctggaata
     5041 caaggggctt catctttcat agatggagtc gttaatgctt ttaagtgggt tttttcagat
     5101 ggagcaggac atagtgtaca taactcagat gcgcaagacc atataattaa agaaacagaa
     5161 aatgagttta agtaatcaat cttttaattt tttcctacgc agaggaagca ttgtattaat
     5221 aataaattta gttctatttc tattgttttt agtgtctaaa aataatgggg ggatgataac
     5281 gtttatgttt ttgatgttta tagttctttt agcagatgta atatatatat gtttcaagaa
     5341 gaaaaatata ttattgtttc tgttgttttt aggatattat tttctactat ttgtggggtt
     5401 ctattggata attaataata ttttattttc agttaaagtt tcaccagttg taatagttga
     5461 atgaaattgg tagtatatca gatcaaaact aaaacaaaat aaacatgaac aaaacagaac
     5521 ggcaaagccc tcaacgaagt taattaacaa aagcgccaac aagtaccata taggaaaccg
     5581 aatcaaaaac ttatcaacaa ccccaaacct ctcacaatct caacaagctt tctatattgc
     5641 tataaaaagt ttacattttt tgtaaagatt tattttattt cgtatatttg aatatgcatt
     5701 tgatagatac aaaaacaaac aaaaggtatg aagttgttgt tgatattgta gatgaacagg
     5761 attttaaaca aattacaaaa tcgaagtttt ttttcaactg gaaagaggaa aagaaaaaca
     5821 tcatttataa actgatgttg gatgatgaaa ttttgggttt gatgtcttgt tttgataatg
     5881 aaggagataa aaggattgaa attaaattgc tttctgtttc taaagaaaat agggggcaaa
     5941 acaaacggta tgaacgaata gcaggaacat taattggtta tgcttgtagg gaagcaacga
     6001 agcgatacgg aatagaagga tgcgtttccc ttgttccgaa aacacagtta aaacatcatt
     6061 acattcaaca atatggtatg atggatgcag gcaaacaagt gtttttggaa ggtgaacaat
     6121 tattaagaat gctaaaagaa tatgagttat gaaaaataga aaattgaacg agaaatacac
     6181 agacaaagaa ttggcagaat cctttgtttt tagagatgat ttaagtgctg aagaaaagaa
     6241 acaatctgat gcagatttga gagaaatacg taataattta cgaagtaaag ttactcctaa
     6301 acaaatgctt ttgtcacgtt tgttgcaatt gaaataccaa attgaagatt acctaaaaaa
     6361 tccgaattat gatgcagaac gctcatttgg attttttctg agaagttacc tggatgcact
     6421 agaaataaaa cacaaggatt ttgcaagtga tataagcatc aatgaaacgg aattgagcca
     6481 aatacttaat aaacatagga atccaagtga aaaaataatt atccgtttag aaattcattc
     6541 caataaaatc ataccagcca tcacatggtt tagactattg gaaaagcaaa gagaatacga
     6601 gataatgaca gataacagtt tgaggattcg cgaaagagat cgtgtaagca atatcctaaa
     6661 agtttcctga ttttcagata acccaaaata gttgattttg tgcatatccc tccccaactc
     6721 caaaaattat ccaaaaaaac actaacttta ggctaagagc ggtatggaga aagacgatga
     6781 gatgaaaagc aagttaacag taattgcttt aaaagccgta aaacacaata aaattataaa
     6841 aagaaaattg ttaaacattt tgtctatatc ttcaatcatc actatacttg gtgttatagc
     6901 cgatagagat tcaactaaac caaatatgtt aatgagattt gtagaatttt tcgtaataat
     6961 tggaattata ttttctataa tttcaattat ttactttaca tctactttta tccataaaaa
     7021 tatccaaaga gcgtaaatgt aaatcttatt taaaatttaa aaacaaccta agaattagta
     7081 tttaaggttg ttttatgttt ctaaaagcaa aaaaacaagc tgaaattatt tgtaaatttg
     7141 ttaagctatt gtaaactgaa atatggcaaa aaataaaaaa atagaagttg aaggcaaaga
     7201 aatcacaata attcttgaca aagaacaaga atatatttcg ctcactgata tgctaaaagc
     7261 caaagacgga gattttttta tttcagattg gcttcgtaac cgaaatactg ttgaatatct
     7321 tggaatttgg gaaagtgttt ataatccaac ttttaattat ggcgaattcg ccacaattaa
     7381 aagtcaggca ggtttgaata aaaaccgtcc aaacatccag tcgggagaaa ttgccgaaag
     7441 gttggcgata ccagcaccaa ccgtaaaacg cattttgtct gagttgctca acaaaggatt
     7501 aattgaaaaa caaggaaacg gacgcaacgt gagttacacg ataaaataac aatgaaaatt
     7561 aatagaacca ataaccaaac taatattaat atcgcccagc tactagcatc ggtttggaaa
     7621 aacaatgtta attgcccaaa tattgccaca aatttcattc aatcggtagg ctaattcatg
     7681 aaacatttgt actttcatcg ccaataaata aaaactatga acgcaacagc tagcaaatca
     7741 gagcaaagca ccaattacag tgccttatcc accttgacaa cggttttctt tttttgggga
     7801 tttatcgcct cgggcaattc ggtgtttatt ccgttttgta aagaatactt cggattggat
     7861 cagtttcaaa gtcagctcat tgattttgca ttttatggcg cctattattt aggagctttg
     7921 gcgttgtttt taatcagctc caagagaaat aaagacctag ttggaacatg gggttataaa
     7981 aaaaccattg tcatcggatt gttgttttca gccattggtg ccggcgcaat gattgtttct
     8041 gtttttagca atgtgttttc ggggatgttg ttgggcttgt ttattgtggc tcttggtttt
     8101 tcgttgcaac aaacatc
//
LOCUS       DAOQVQ010000053         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9840_length_8092_cov_4.807142, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000053 DAOQVQ010000000
VERSION     DAOQVQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9840_length_8092_cov_4.807142"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN22_02850"
     CDS             complement(<1..874)
                     /locus_tag="PKN22_02850"
                     /inference="COORDINATES: protein motif:HMM:NF033831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HNS41673.1"
                     /translation="MYQPYIRGKQFELIGIRELTSSVLSPNKEKVSPIIEPVKDSSTL
                     KTTLKELVSFNVNFTIIANPQVGSFRDIEAIFSAIKSSVGESKNYQVGIIFHNKIVHA
                     DTLEILRKYSDVIPQLTIIHNATFDNVFDILKLYQEYFTIKYNVINLSATSKRYHRNF
                     EESTRVELDDYFNAQSKNSDYLHVNESNFSEEHIYYKEEGFIGFSDYLSIGEEYSETG
                     FLPYAVAIHLSYSDDNNKIKIKHFVSDNNDDTSDIAGKFAEALDKLITWCDQTNYNSV
                     AIPQFREYHTNGHFP"
     gene            complement(878..1717)
                     /locus_tag="PKN22_02855"
     CDS             complement(878..1717)
                     /locus_tag="PKN22_02855"
                     /inference="COORDINATES: protein motif:HMM:NF033832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HNS41674.1"
                     /translation="MQNLRVLSQIFSPTMFQKIVREDDFALFKKQTMKHFNTISYNTN
                     LDLISSLYKTLQQKYRCEYIYKNHLFLEIFKKEKLESTLLLNELKIGTSKADLVLLNG
                     SIRIYEIKTELDGLGKLSKQLNDYQKFADKVYVVTDEKFAEKIKVEYANTNIGIIVLD
                     NKNKLETIKEANENFSLFEFDTIFKILRKQEYLELVFDNYKFIPDVPNTKIFKASYEL
                     LAKMDIVDFQKQVLSKLKERKLLNPDLLKSRNTPKELKHICNTLDFNEQEYQKLYNFL
                     ATK"
     gene            complement(1816..2547)
                     /gene="truA"
                     /locus_tag="PKN22_02860"
     CDS             complement(1816..2547)
                     /gene="truA"
                     /locus_tag="PKN22_02860"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNS41675.1"
                     /translation="MKRYFFEVAYDGGAFFGWQVQIGQSSVQQTIENALTKLNSNIHV
                     PIVGCGRTDAGVHAKCSTFHADLELKIDLDKLQYRLNKILPSSISILRIKEVDGELHA
                     RFSATKRTYRYFIHTQKNPFKHNYSTYLPGDLNLEKMNEACQLLLGTHDFTTFSKANT
                     DVKTHICTVFKAEWSTTEDGYVFEYAANRFLRNMVRATVGTLLLVGQEKITIEAFKNI
                     FESLDRSQCASSAPAQGLYLWQVEY"
     gene            complement(2556..3929)
                     /locus_tag="PKN22_02865"
     CDS             complement(2556..3929)
                     /locus_tag="PKN22_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41676.1"
                     /translation="MTRILILSFVCFFVASSFAAMTPPDSTATIAQKTAAGYMIEEGK
                     ALFNSGRYRDALNKFRDAAKKDVTSAKATLWISRCHYELSNYGYALRYAEDARLLSGI
                     SQEKELYEMLAEANHRLGNLDSAIANYEKGIQVMPKKRAADLELPLHLEQAKYAKEQL
                     AKGIKNTRIHLNGDVNSGYNDYSPVIYNGGNSMYFTSRRNNTTGGRQNPSDQQFFEDI
                     YQAKWNSATNQWDSVTNRIGKINGEGFESISYVSEDGLVAYVTMNYSEAMETKFTTKS
                     SDIAEVKWTKQDRWSAPRLLGKNINSSFYDGNATLTADGQTMYFVSERNANKKKSDIY
                     VAYKTGNDWGSAVALPAEINSKGRETTPYITPDGRYLFFSSNGHKNSLGGYDVYVVEC
                     TGGKSWGPVKNLGPAINTVNDDFGFKIYDATGKAYINGMEVIGDKASLDIYEFNLRLA
                     DLIKSLD"
     gene            complement(4008..4835)
                     /locus_tag="PKN22_02870"
     CDS             complement(4008..4835)
                     /locus_tag="PKN22_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNS41677.1"
                     /translation="MIQFFKEFWLGITSYWTAFKFINQHKLYWYFLVPAGFMLGIYFL
                     GNLILNFKITPNLTNMNGIVWYFIHVAFNISIALLLMKFTKYIVVITLSPLLSHLSQK
                     CERIITGKTYPIDVKQIIKDVKRGIRLAFRNIIWEYSFFLLIIIISWIGWGDPLGSPL
                     LYLTFIVGFYYYGFSFIDYDNERRKLDEQKSIEFVRSHRGLAMGIGMIYSFLILVPVD
                     LEVLFSITELSQHGFWHIFAILFQLLLWICASIAPILAIVSASIAMIKVNKTETVVG"
     gene            complement(4839..5255)
                     /locus_tag="PKN22_02875"
     CDS             complement(4839..5255)
                     /locus_tag="PKN22_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNS41678.1"
                     /translation="MITRKEKFTSESSGNFNKIIEGTKIVGDVIADQNMQIDGEIKGN
                     ITTSAKIFIGENGVVNGNLTCVEAEIEGTINGTLSVEGLLTLRNTSKINGDIFTTRIQ
                     IDEGAAFNGVCNMGAKPSTSRMQPTPENEPISLDFN"
     gene            complement(5257..7965)
                     /locus_tag="PKN22_02880"
     CDS             complement(5257..7965)
                     /locus_tag="PKN22_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41679.1"
                     /translation="MNKYNRLFLIFFSLVALLYACSTEKNTAVNRAYHYVTARYNGNF
                     NANELINVSLTTYRDNLSEDFHQLIPVEAVPNETEVEAFYPALDTAISKVKTVIADHS
                     MPTLDKPSRKNAEHNNYIDENWITIGRAYYYRRDYDIALKNFKFINRYFSNDPSNYVG
                     EVWMAKTNIRTGNYTDAKLSLDKVDQVLKAQEEASKNKGEASKKSKKSKAAKLNKSGG
                     SSKGKGKNEDKVAKFPKRLKFELERTKADLAIAKQEYPKAIEYLESALKEAKTSADKG
                     RLNFILGQLYEQDSKTDLAIAKYKKSTKYKIPYPMSFSAQLKSSILDGGPKIKKSLNK
                     MLKDAKNAEFKDQIYYAQAMISIKEGNEDKAMEQLTNSAFFSTNNVRQKGMAYEKMGD
                     MRYAKRDYVKAQKYYDSCVVTVTEKYPNYFAIKTKAEKLADLVVAVETAQYEDSIQRI
                     AKLSPDEQEKFLKNVIKKQEEEAAAKAKREAEKLRELAKNNNAFAQDMNAKGGGYWGN
                     SASITQGATDFQKQWGNRPNEDNWRRSNKTIAAPPSNTDNGSDTSAVASTDSAGAQQN
                     AGLTVESLAKGLPQTEDDFKKSNERLVEALYNAGIIYKEQLKEPGLAKTQFHRILDKE
                     FESDYNLMAAYQLYKMYENEDPTKAYAQKEYILNNYPNSDYANYMRDPDYFVKKKELD
                     ALREQEYVTILNRYNRKIYYPVISKGEQVMDNEPDNEYRAKYMLLLAMSKGQVTEDKQ
                     ELVPILERIVKEYPNSDEASRATDMLNIIKNGYSKNEPVVFGNKTIYSYEEKVPHIVL
                     IFLDPKENLDLSKSKVFDFTREFFPKSKVKVSTNLYNSTQNILMVAEFAKEADAKEYV
                     NTFKKTKKYLLDLRESKTVVISPKNMKLLFETKELEQYELFYTEFY"
BASE COUNT         2422 a         1457 c         1309 g         2904 t
ORIGIN      
        1 ccggaaagtg tccgtttgta tggtattctc gaaattgcgg aatagcaaca gaattgtaat
       61 ttgtttggtc gcaccaagta attaatttat ctaatgcttc cgcaaactta cctgcaatat
      121 cagaagtatc atcattgtta tcagaaacaa aatgtttaat ttttatttta ttgttatcat
      181 ctgaataaga caaatgaata gctaccgcat aaggtaaaaa acctgtttct gaatattctt
      241 caccaatgga aagatagtct gaaaacccaa taaatccctc ttctttatag taaatatgtt
      301 cctctgaaaa attactttca ttaacgtgta aataatccga attttttgat tgagcattga
      361 aataatcatc aagttcaaca cgtgtgcttt cctcaaaatt cctatggtat cttttgcttg
      421 ttgctgacaa gtttattaca ttatatttga tagtaaaata ctcttgatat agttttagaa
      481 tatcaaaaac attatcaaaa gtagcattat gaataatggt taattgagga attacatcag
      541 aatattttcg taaaatctct aaagtatcgg catgaacaat tttattatga aaaataatac
      601 cgacttgata atttttactt tctccaacag aacttttaat agcagaaaaa atagcctcaa
      661 tatctcgaaa tgacccaact tgtggattag caataattgt aaaattcaca ttaaaactga
      721 ctaactcctt taaagtagtt ttcaatgttg aagaatcctt aacgggttca ataattggcg
      781 aaactttttc tttgtttggc gacaaaacag aagatgttaa ttccctaata cctatcaatt
      841 caaactgttt tcctcttata tacggttgat acatactcta ttttgttgct aaaaagttat
      901 ataatttctg atattcctgc tcgttaaaat ccaaagtatt acaaatgtgc tttaactctt
      961 ttggtgtgtt tcttgatttc aacaagtcgg gatttagcaa ttttcgttct ttaagtttac
     1021 ttaaaacttg cttttgaaaa tcgactatat ccattttagc aagcaattca taactagctt
     1081 tgaatatttt tgtattgggt acatcaggaa taaatttgta attatcaaaa accaattcaa
     1141 gatattcttg ttttcttaaa attttaaaaa ttgtatcaaa ctcaaacaaa gaaaaatttt
     1201 cgtttgcctc cttgatagtt tctaatttgt ttttattatc aagaacaata attccaatgt
     1261 ttgtattcgc atattcaact ttaatttttt ctgcaaactt ttcatcagta acaacataaa
     1321 ctttatccgc aaacttttga taatcgttta attgtttgga caacttccct aaaccatcaa
     1381 gctctgtttt gatttcataa attcttatag aaccgtttaa caaaaccaaa tcagccttag
     1441 aggtacctat ttttaactca ttgagtaata aagttgactc taacttttct tttttaaaaa
     1501 tttcaagaaa taaatggttc ttatagatat attcgcatct atatttctgt tgtaacgttt
     1561 tatataaaga gctgattaga tctaagttag tgttgtaaga gatagtgtta aagtgtttca
     1621 tagtttgttt tttaaacaaa gcaaaatcat cttctcgcac tatcttttga aacatagttg
     1681 gagaaaaaat ttgagataaa actcttagat tttgcataaa cactactatt tcttttacag
     1741 aacaaatata acaatttaat ataagatttt cacatttcaa caatagaaac taacggtata
     1801 ctgttaattt gcaaatcaat attcaacctg ccataaatac aaaccttgag ctggagcaga
     1861 ggaggcgcac tgacttctgt ccaacgattc aaagatgttt ttaaatgctt caatagtaat
     1921 tttttcttgc cctactaaaa gcaaagttcc caccgttgca cgcaccatgt ttctcaaaaa
     1981 ccgattggcg gcatattcaa acacgtatcc atcttctgtg gttgaccatt cggctttaaa
     2041 cacggtacag atgtgtgttt taacatctgt attggcttta gaaaaagtag taaaatcgtg
     2101 tgtgcctaat aatagttggc aagcttcgtt cattttttct aggtttaaat cgcctggcaa
     2161 ataggttgaa taattatgtt taaacggatt cttttgtgtg tgtatgaaat agcgataggt
     2221 gcgttttgtt gcagaaaaac gagcgtgcaa ttcaccatcc acttctttaa tacgtagtat
     2281 ggaaatggag gagggcaaaa ttttattgag tcggtattgc agtttatcca aatcaatttt
     2341 taactctaaa tcggcatgaa aagtagaaca tttggcatgc acacctgcat cagttcttcc
     2401 acagccaaca atggggacgt gaatattgga gtttaatttg gtgagtgcat tttctattgt
     2461 ttgttgaaca gaggactgac caatttgcac ttgccagcca aaaaacgctc cgccatcata
     2521 ggcaacctca aaaaagtaac gtttcaccga gttttttaat ctaacgattt gataagatct
     2581 gccaaacgaa ggttgaattc ataaatatct aaagacgctt tatcgccaat tacttccata
     2641 ccattgatgt aagctttacc tgtagcatcg taaattttaa agccaaaatc atcgttaaca
     2701 gtattgatag ctggccccaa attttttaca ggtccccacg atttcccacc agtgcattca
     2761 acaacatata cgtcgtatcc accaagtgaa tttttgtgac cattggagct aaaaaataaa
     2821 taacgaccat ctggtgtgat ataaggagtt gtttcgcgac ctttagaatt gatttcagca
     2881 ggtaatgcaa cggcacttcc ccaatcgtta ccagttttat aagctacata aatatcactt
     2941 ttctttttat tagcattgcg ctcagaaaca aaatacattg tttgcccatc agcagtcaat
     3001 gtagcatttc catcgtagaa cgaagaattg atgttttttc caagtaagcg cggtgccgac
     3061 catctgtctt gtttggtcca tttcacttca gcaatatccg aacttttggt ggtaaattta
     3121 gtttccattg cttcagaata attcattgtt acataagcaa ctaaaccatc ttctgacaca
     3181 taggaaatag actcaaatcc ttctccgtta atttttccaa tacggttagt aacgctgtcc
     3241 cactgattag ttgcgctgtt ccattttgct tgataaatat cttcaaaaaa ttgttgatcg
     3301 gatgggtttt gacgcccacc agttgtgttg tttcttctcg aagtaaaata catggagttt
     3361 ccaccattat aaattacggg agaataatca ttgtatcccg aattaacatc gccattaaga
     3421 tgaatgcgcg tgtttttaat tccttttgcc aattgttctt ttgcatattt tgcttgttcc
     3481 aaatgcaaag gcaattccaa atccgcagct cttttctttg gcatcacttg aattcctttt
     3541 tcatagttag caatggcaga atcaagattg cctaaacggt ggttggcttc tgctaacatt
     3601 tcatacaatt ctttttcttg agaaatacca ctcaacaaac gtgcatcttc agcatatcgt
     3661 agggcatagc cgtagttgga aagttcataa tgacatctac ttatccataa tgttgccttt
     3721 gcagaagtta catctttttt agcagcatct ctaaatttat tcaacgcatc gcggtatcta
     3781 ccactgttaa acaaagcttt tccttcttca atcatataac cagcagctgt tttttgagca
     3841 atggttgctg tagaatctgg cggagtcatt gcagcaaatg aagaagcaac aaagaagcaa
     3901 acaaaagata aaattagaat tcgagtcata gttaactttt tattatactg caaagtaaaa
     3961 caattttttt gaattttctg gtaaaagtat aacgaactct gtttttacta tccaacaaca
     4021 gtttctgttt tgttaacttt tatcatagca atagaagccg aaacgatggc taaaattggt
     4081 gcaatggaag cacaaatcca taaaagcaat tgaaaaagaa tggcaaatat atgccaaaag
     4141 ccatgttgcg atagttcggt tatggaaaac aatacttcta gatctactgg aaccaaaatc
     4201 aaaaaagaat aaatcattcc aatacccatt gctaaaccac ggtgagagcg cacaaactca
     4261 atgctttttt gttcatctag tttcctgcgt tcattgtcgt aatcaataaa actaaagcca
     4321 tagtaataaa agccaacaat gaaggtgaga tacaacaacg gtgaaccaag tgggtcgccc
     4381 caacctatcc atgaaatgat gataatgagt agaaaaaaac tgtattccca aatgatgttt
     4441 ctaaatgcta ggcgaattcc tcgtttaaca tctttgataa tttgttttac atcaatagga
     4501 taggtttttc cggtgatgat gcgttcgcat ttttgactca agtgcgataa caaaggggaa
     4561 agtgtgatga caacaatgta tttggtaaat ttcatcaaca acaaggcaat ggagatatta
     4621 aacgccacgt gaatgaaata ccacacaatg ccgttcatgt tggttaaatt gggtgttatt
     4681 ttgaaattta aaattaggtt tcccaaaaaa taaataccca acataaatcc agctggcacc
     4741 aaaaaatacc aatataattt gtgttgatta ataaatttaa aagctgtcca atacgaagtt
     4801 attcctaacc aaaattcctt gaaaaattgt atcatttttt agttgaaatc aagcgaaatg
     4861 ggttcgtttt ctggtgttgg ttgcattctt gaagttgacg gttttgcgcc catgttgcac
     4921 acgccgttga aagcagcgcc ttcgtcaatt tgaattctgg ttgtaaaaat atctccgttg
     4981 attttagagg tgttgcgcaa tgtcaacagt ccttcaacgg aaagtgttcc gttaattgtt
     5041 ccttcaattt cggcttcaac acatgttaaa ttaccattca caacgccgtt ttcaccaata
     5101 aaaattttag cactggtggt aatgtttcct ttaatttcgc catcaatttg catgttttga
     5161 tcggcaataa catctccaac aattttagtt ccttcaatga ttttattgaa atttcccgaa
     5221 ctttctgaag tgaatttttc tttacgtgta atcatattag tagaattctg tgtaaaacaa
     5281 ttcgtattgc tccaattctt ttgtttcaaa caacaatttc atgtttttgg ggcttataac
     5341 aacagttttt gattcacgta aatctaataa atatttttta gttttcttaa acgtattgac
     5401 atattctttg gcatcggctt ctttagcaaa ttcggcaacc attaaaatat tttgtgtgct
     5461 gttgtataag ttggtggaaa cttttacttt gctttttggg aaaaactcac gcgtaaaatc
     5521 aaaaactttt gacttcgata aatctaaatt ttctttcgga tcaaggaata ttaaaacaat
     5581 gtgtggcact ttttcttcat aagaatagat ggttttatta ccaaacacaa caggttcgtt
     5641 tttagaataa ccatttttaa taatattcaa catatctgtt gctcttgagg cttcatcaga
     5701 atttggatat tctttaacaa tgcgttccaa aattggcact aactcttgtt tatcttctgt
     5761 aacttgccct ttactcatag ccaacaacaa catgtattta gctcggtact cgttatcagg
     5821 ttcgttgtcc attacttgct caccttttga gataacaggg taataaattt tacggttgta
     5881 acggttcaag atggttacat attcttgttc acgcaaggca tccaattctt ttttcttcac
     5941 aaaataatcg ggatcgcgca tgtagttggc ataatccgaa tttggataat tgtttagtat
     6001 gtattctttt tgtgcataag cctttgttgg gtcttcattt tcatacattt tatacaattg
     6061 ataggcggcc attaaattgt aatccgattc aaattcttta tccaaaatac ggtggaattg
     6121 cgttttagcc aaaccaggtt cttttaattg ctctttgtag atgatcccag cattgtaaag
     6181 tgcttccacc aatcgttcgt ttgatttttt gaaatcgtct tcggtttgcg gtaatccttt
     6241 cgccaatgat tcaaccgtaa ggcccgcatt ttgttgcgct ccagcactat cagttgatgc
     6301 aacagcgctt gtatcgctac cattatccgt attggatgga ggtgcagcaa tggttttgtt
     6361 gcttcttctc caattatctt cattaggacg attaccccat tgtttttgga agtcggttgc
     6421 accttgagtt atagatgcac tattgcccca atatccaccg ccttttgcgt tcatatcttg
     6481 tgcaaatgcg ttgttgtttt tagccaattc tcgtagtttc tctgcttcgc gttttgcttt
     6541 tgctgccgct tcttcttctt gtttcttgat aacgtttttc aagaattttt cttgttcatc
     6601 ggggcttaat ttggcaatgc gttggatgct gtcttcatat tgagctgtct ccactgccac
     6661 cactaaatcg gctaactttt cagcctttgt tttaatagca aaataatttg gatatttttc
     6721 ggtgacagta actacacatg aatcgtaata tttttgtgct tttacgtagt cgcgtttagc
     6781 atagcgcata tctcccattt tttcatacgc cattcctttt tgacgtacat tattggttga
     6841 aaagaaggca gaattagtca actgttccat tgctttatct tcgtttcctt ctttgatgct
     6901 aatcattgct tgcgcataat agatttgatc cttaaattca gcatttttag catcttttag
     6961 catcttatta aggctctttt tgatttttgg gccaccatct aaaatagaag attttaattg
     7021 agcactaaaa ctcattggat aaggaatctt gtatttagtt gactttttat attttgcaat
     7081 ggctaaatct gttttgctgt cttgctcgta tagctgacct aatataaagt taagtcgtcc
     7141 tttatctgcc gaagttttcg cttctttcag tgcactttct aaatattcaa tagcttttgg
     7201 atattcttgc tttgcaatgg ctaaatctgc ttttgtgcgc tctaattcaa attttaatcg
     7261 ttttggaaat tttgctactt tatcttcgtt ttttcctttt cctttagaac taccacctga
     7321 tttattgagt tttgcagctt ttgatttttt cgattttttt gacgcttctc ctttgttttt
     7381 tgacgcttct tcttgtgctt ttaacacttg gtcaactttg tctaagctta atttagcatc
     7441 ggtatagtta cctgtgcgta tattggtttt agccatccat acttcgccca cataattaga
     7501 agggtcgttt gaaaaatagc ggttaataaa cttaaagttt ttcaacgcta tatcgtaatc
     7561 tcttctgtaa taataagctc taccgatggt aatccagttt tcgtcaatgt aattgttgtg
     7621 ttcagcattt tttcgtgatg gcttatccaa agtgggcata ctatggtcgg caattactgt
     7681 tttcaccttt gaaatggctg tatccaatgc tggataaaac gcttcaacct ctgtttcatt
     7741 cggtacagct tcaacgggta ttaattgatg aaaatcttca cttaaattat cgcgataagt
     7801 agttagcgat acatttatta actcattggc attaaaatta ccgttgtaac gagcagtcac
     7861 atagtgatat gcacggttta cagccgtgtt tttttcagta gagcatgcgt agagtagtgc
     7921 tactaacgaa aaaaagatta aaaataagcg gttgtattta ttcattcacg atgttgctaa
     7981 agctcattaa ttaacagcaa aaattcattt ttattgtgtg ctttacgcaa tattggtaaa
     8041 aacttgttga atatcgtcgt cgtcttcaat tttatccaac attttttcaa ta
//
LOCUS       DAOQVQ010000054         8026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_9940_length_8026_cov_4.755614, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000054 DAOQVQ010000000
VERSION     DAOQVQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9940_length_8026_cov_4.755614"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..2253
                     /locus_tag="PKN22_02885"
     CDS             19..2253
                     /locus_tag="PKN22_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS41680.1"
                     /translation="MHAAGYVADSLKISDSTITTTILLRPINAILEGVVVTGSTRASS
                     IKENPIAILAVSTKQLDRAVENNIVDALVKNVPGLNVVKTGPNISKPFIHGLGYNRVL
                     TLYDGIRQEGQQYGDEHGLEVDDYNMDKAEVIKGPASLLYGSDAIAGVVSLFPAIPRL
                     NDGLLHGKFTSEYQTNNGLIGNGLRLNYAKNGWLFALRGSYKMAKNYQNPVDGRVYLT
                     NFNVGNFSALAGHQSKYGYTHLNFTLYDNHQGIPDGSRDSLTRQFTKQVFEGDQDDIT
                     NRPILTQKELNSYKTPDLAQHIQHYRVYAHSFYRVGKGSIDVLLGVQQNRRREYTHPS
                     LPKQAGMYMQLNTMNYGFRYNAPDFSNISISVGINGMVQQNKNKDATDFPIPDYHLLD
                     GGAYLYAKWKKNKWSISGGLRYDWRYIRWNDFYVGTNPTTGFDQQVAANSSAYLQFQA
                     YEKLFQGMSGSVGATYQPTENISLKANVGRAYRSPNVTEIGSNGLDPGAHIVYLGNRN
                     FKPEFSLQEDIGIQLDFPHFSASAGLFNNNIQNYIYMSSVADEHGTIVVDAQGNKTYQ
                     YQQSKAHLLGADVGLNIHPVPLKGMQWNSSLTLVYGFNKNSQFKGKGVDGEYLPLIPP
                     LTIRSNLAYEWTIKAKKPIKITPSIEVEFSAKQHRYLSLNGTETYTPDYTLLHTGVNV
                     EFFYNPEHSIQLIVQANNVLNRAYQSHLSRLKYFEYYSQTPNNRSGIYNMGRNLSIKL
                     IVTF"
     gene            2413..3123
                     /locus_tag="PKN22_02890"
     CDS             2413..3123
                     /locus_tag="PKN22_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNS41681.1"
                     /translation="MKIEVWSDIMCPFCYIGKRNYEEALAKFKHADRIELVWKSFQLD
                     PTTPTTKVEQNTYEYLAARKGWSMKECIDIHQSVTKTAKKAGLTYNFDNVKVANSFNA
                     HRIIQLAKTKGLGDAAEEQFFKAHFTDGRDLNDKNELITIASFIGLSEQEVDEALNNQ
                     AFADAVHADIQEAAQLGIQGVPFFVMNRKYAVSGAQAPTVFLQTIEKAFEEWQKENPA
                     TPFEVIEGKTCKPTGECN"
     gene            3126..5078
                     /locus_tag="PKN22_02895"
     CDS             3126..5078
                     /locus_tag="PKN22_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS41682.1"
                     /translation="MSHQHSHPEQEKCCSATAHQHHEHEHNHEHGGSVFSLFQQPLIS
                     FCLLLLGIGMDYFQPAWFTDWVRVAWYFSAYVPVGMPVLKEAIESIRNNDFFSEFFLM
                     TIATIGAFAIGQYPEAVAVMLFYSIGEAFQTMAVSKAKGNIKALLDQRPDEVTIIRNG
                     QTQTVHAQEVEIGAVVQLKPGEKVALDGILLSETAAFNTAALTGESVPDTKQQGEEVL
                     AGMLNLQSACQIQVTKAYNDSKLSKILELVQNASAQKAPTELFIRKFAKIYTPLVVLS
                     AVLICLLPFFFVDHYRFSEWLYRSLIFLVISCPCALVISIPLGYFGGIGAASRSGILV
                     KGSNFLDLLASVDTVVMDKTGTLTEGVFSVQEVVFKDSFNQDEILKLVNAVEQLSSHP
                     IATAIHEYVGAVDNSIVLENTEEIAGHGIKTTVNGKELLVGNFKLLHKMQVDVDFDAS
                     NLVFTTIAVAYDRKYVGYITIADQIKKDALATVQALQSMKVSAVMLSGDKTAVVQKVA
                     SSIQIAHAFGDLLPADKVEKVKNLIQQGKKVAFVGDGLNDAPVIALSDVGIAMGGLGS
                     DVSIEAADVIIQDDQPSKIPVAIQIGKATKRIVWQNIAFAFGVKLIVLLLGAGGIATM
                     WEAVFADVGVALLAILNAVRIQRMTF"
     gene            complement(5171..6331)
                     /locus_tag="PKN22_02900"
     CDS             complement(5171..6331)
                     /locus_tag="PKN22_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41683.1"
                     /translation="MKKYYYIAFCANFSFAFSKQLFSQVVNCPYNTPEFPFETQDRWS
                     GSSYSWSAGLYMPSQIGGAQTINSISYRLDNTFGAGGTYNNIYVYLRHTAVTNYASSP
                     GYPGTGGFTQVYNGSVVYPGTNGTTITITFSTPFVYNGTSNLEVLFENQGGHWDSAKP
                     WFDRTNDAGSGVFPGKVGAGGTWAIATGVSSNRQFNLAIFTGVSNTFCGAYPLAVTLK
                     DFSATCEGNQFVLDWETASEKNNDYFLVDYSEDGTTWTEVGRIDGNGSTTIEQKYSFN
                     HRQQSNSTTYYRLTQVDFDGTQEILRTISANCVNKDEVVVSPNPTNGQISVFNVQKGA
                     IVTAYSALGTQLSSTVAETNFVNFDLSNHPARVYFINVQSENNQKTLKVTLQ"
     gene            complement(6458..7471)
                     /locus_tag="PKN22_02905"
     CDS             complement(6458..7471)
                     /locus_tag="PKN22_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNS41684.1"
                     /translation="MVNWFNYKKFLVVVLPLLLLSLSLVEEPTLRAKRWGFYGHKRIN
                     RMAVFTLPPPMFGFYKDHIEYVTENAPNPDRRRYAVEGEAQRHFIDLDHYYQPGDDPS
                     KAMPRRWDDAVAKYTEDTLQAYGIVPWVIVRYKYSLQKAFEEGNVDNILRISADLGHY
                     MSDAHVPLHTTENYNGQMTNQKGIHGLWESRLVEINADDYDYFVGKVKYIKNPLDFAW
                     DVVLASHRSVDSVFRIEKELTQEFGEDKKYSYEQRGNITTKVYSYEFSQAYHQRMNGM
                     VERRMKAAIIATGSIWYTAWVDAGQPNLEQLRNKPVSVELQQESEALEHLEHGKSKGR
                     ICD"
     gene            complement(7449..>8026)
                     /locus_tag="PKN22_02910"
     CDS             complement(7449..>8026)
                     /locus_tag="PKN22_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNS41685.1"
                     /translation="IIPVPTIEEALAYKEKSYLVGAERNGQIVEGFDFGNSPYSYMRE
                     DFKGQEVVLSTTNGTKALNIAKDAEMVLVGSFLNLDALCQFLATQDKNILCLCSGWQD
                     KFNLEDTICAGAISEYLLATGNFTSDEDSSVAAKYLYISAKNNYFGYLKSSSHRRRLK
                     NLNLNEDVKYCLTPNQTNVIPILKDGKLVQL"
BASE COUNT         2477 a         1520 c         1620 g         2409 t
ORIGIN      
        1 tttccaacgc atcttcaaat tcatgctgca ggatatgttg cagattcact aaaaatttct
       61 gattcaacta tcactacaac cattttattg cgacccatca acgcaatttt ggaaggagtg
      121 gttgtaacag gctccacgag ggcatcctcc atcaaagaaa accccattgc aattttggca
      181 gtttcaacta agcaattgga tagagcagta gaaaacaaca ttgtagatgc attggtgaaa
      241 aatgtaccgg gactcaatgt tgtgaaaaca ggacccaaca tttccaaacc atttattcac
      301 ggtttgggct ataatcgggt gttgaccttg tatgatggta ttcgccaaga agggcaacaa
      361 tacggcgatg agcatgggtt ggaagtggac gactacaaca tggataaggc cgaagtaatt
      421 aaaggtccgg caagtttgct ttatggctcc gacgcaattg ctggagtggt gagtttgttt
      481 cctgcaattc cgcgactaaa cgatggatta ttgcatggaa aattcacgtc agagtaccaa
      541 accaacaacg gattaatagg caatggcttg cgcttaaatt atgcaaaaaa tggttggctg
      601 tttgccttgc ggggttctta taaaatggca aaaaactatc aaaacccagt ggatggcagg
      661 gtgtatctca ccaattttaa cgttggaaat ttttctgcat tagcaggtca tcagtctaaa
      721 tacggttaca cccatttgaa tttcacgttg tacgataatc atcaaggaat tccagatggt
      781 agccgcgatt cgcttacacg tcaatttacc aaacaagttt ttgaaggcga tcaagatgac
      841 attaccaacc gtccaatttt gacacagaag gaattaaatt catataaaac tcccgatttg
      901 gcacaacata tccaacatta tcgcgtgtat gcgcatagtt tctatcgagt aggtaagggg
      961 agcatagacg tattgttggg agtgcaacaa aaccgacgaa gagaatacac gcatccaagt
     1021 ttaccgaaac aagctggaat gtacatgcaa ctcaacacca tgaattatgg ttttcgttac
     1081 aatgcacccg atttttccaa tatttccatt tctgttggta ttaatggaat ggtgcaacaa
     1141 aataaaaata aagacgctac agattttccc attcccgact accacttatt ggatggaggt
     1201 gcttatttgt atgccaaatg gaaaaaaaat aaatggagca taagcggagg tttgcgttac
     1261 gattggcgtt acatccgttg gaatgatttt tatgtcggaa ccaacccaac aaccggtttt
     1321 gaccagcaag tagcagccaa ttcttctgcc tatttgcaat ttcaagccta tgaaaaattg
     1381 tttcaaggaa tgagcggtag tgtaggagca acttatcaac caacagaaaa cattagtttg
     1441 aaagcaaatg taggaagagc ttatcgatcg cccaatgtta cagaaattgg gagcaatggc
     1501 ttagacccgg gtgctcacat tgtttatttg ggaaatagaa actttaaacc cgaattttcg
     1561 ttgcaagaag acataggcat tcagttggat ttccctcatt tttcggctag tgcaggtttg
     1621 tttaacaaca acattcaaaa ctacatttat atgtcgtcgg ttgccgatga acatggaact
     1681 attgttgtgg acgcgcaagg caataaaacc tatcaatacc aacaatccaa agcacatttg
     1741 ttgggtgccg atgttgggtt gaacattcat cctgttccgc ttaaagggat gcaatggaat
     1801 tctagtctaa cattggttta tggattcaac aaaaattcac aattcaaagg taaaggtgtt
     1861 gatggagaat atttaccctt gattccaccg ttaaccattc gctccaactt ggcgtatgaa
     1921 tggacgatta aggcaaaaaa accaataaaa attacaccaa gtatagaagt agaatttagt
     1981 gccaagcaac accgttatct aagtttaaat ggaacagaaa cctacacacc cgattacacg
     2041 ttgttgcata cgggcgtaaa tgttgagttt ttttacaacc cagaacattc cattcaatta
     2101 atcgtgcaag ccaataatgt attgaacaga gcctatcaat cgcatttaag tcgcttaaaa
     2161 tattttgaat attacagtca aacgcccaac aatcgttctg gaatttacaa catgggacgc
     2221 aatttaagca tcaaactcat cgttacattt tagcaacaca agcttttttt gatagaaaag
     2281 ttcgtaaatt tgaaagagat taaagttaca acaatgttaa aacaaacaac tttactgttg
     2341 atagttagta tttttatagg atggaatggg ttttcacaac aagaaaacgc aaccactaaa
     2401 aaagagaata aaatgaaaat tgaagtttgg agcgacatca tgtgtccgtt ttgctacatc
     2461 ggcaaacgaa attacgaaga agcattggca aaatttaaac acgccgatcg cattgagttg
     2521 gtttggaaaa gttttcagtt agacccaaca acacccacca caaaagttga acaaaatacc
     2581 tatgaatact tggcagcacg caaaggttgg tcgatgaaag aatgtataga catccatcaa
     2641 tcggtaacta aaaccgccaa aaaagcagga ttaacctata attttgacaa tgtaaaagtt
     2701 gccaattcgt tcaatgcaca ccgcatcatt cagttggcaa aaactaaagg tttgggcgat
     2761 gcggcggaag aacaattttt taaagctcac tttaccgatg gtcgcgattt aaacgataaa
     2821 aacgagttga taaccattgc ttctttcatc ggactttctg agcaagaagt ggatgaagcg
     2881 ttgaacaatc aagcatttgc tgatgctgtt catgcagata tacaagaagc tgcacaattg
     2941 ggcatacaag gtgttccgtt ttttgtgatg aatagaaaat atgctgtttc tggtgcacaa
     3001 gcaccaaccg tttttttgca aacaatagaa aaagcatttg aagaatggca aaaagaaaac
     3061 cctgctaccc catttgaagt gattgaggga aaaacttgca agccaactgg cgaatgtaac
     3121 tagttatgag ccaccaacat tcccatcccg agcaagaaaa gtgttgttct gcaacagcgc
     3181 atcaacacca cgaacacgag cataaccatg aacacggagg aagtgttttt tccttatttc
     3241 aacaacctct tatttcgttt tgtttgttgc ttttgggtat tggaatggat tattttcaac
     3301 ccgcttggtt tacagattgg gtgcgtgtgg cgtggtattt ttcggcctac gttccagtgg
     3361 gaatgcccgt tttgaaagaa gcgatagaaa gcatacgaaa taatgatttt ttctcagaat
     3421 ttttcttgat gaccattgcc actattggtg cttttgccat tggtcagtat ccagaagcag
     3481 ttgctgtgat gttgttttat tccattggcg aagcatttca aacaatggct gtttcaaaag
     3541 caaaaggaaa tattaaggct ttgttggacc aacgaccaga tgaggtgaca attatccgca
     3601 acggacaaac gcaaacggtg catgctcaag aagttgaaat tggtgcagtt gttcaattaa
     3661 aacctggtga aaaggtagca ttggacggta ttttattgtc agaaacggca gcgtttaaca
     3721 ccgcagcatt gacaggagaa agtgttccag ataccaaaca gcaaggcgag gaagttttgg
     3781 cgggtatgtt gaaccttcaa tcggcttgtc aaattcaagt taccaaagca tacaacgata
     3841 gtaaactgag taaaatattg gaattggtgc aaaacgcaag tgcacaaaaa gcaccaactg
     3901 aattgtttat ccgaaaattt gcaaaaatat acacgccatt ggtggtgttg tcggctgttt
     3961 taatttgctt gttgccattt ttctttgttg accattatcg tttttctgaa tggttgtatc
     4021 gttcacttat ttttctagtt atttcttgcc cgtgtgcctt ggtaatttcc attccattgg
     4081 gttattttgg tggaattggg gcggcaagta gatctggaat tttggtaaaa ggtagtaatt
     4141 tcttggattt gttagcatcg gtggatacgg ttgtgatgga caaaacagga acgcttaccg
     4201 aaggtgtttt ttcggtgcaa gaggtggttt ttaaagacag ctttaaccaa gacgagattt
     4261 taaaattggt gaatgcggtg gaacagttga gcagtcaccc cattgcaaca gccattcacg
     4321 aatatgtggg ggctgttgat aattccattg tgttggaaaa caccgaagaa attgctggtc
     4381 acggcattaa aacaacggtg aatgggaaag aattgttggt tggtaatttt aaattgctcc
     4441 acaaaatgca agttgacgtt gattttgatg ctagcaactt ggtatttacc accattgctg
     4501 ttgcttacga tagaaaatac gtgggttaca tcaccattgc cgatcaaatt aaaaaagatg
     4561 cattggcaac ggtgcaagct ttacaatcaa tgaaagttag cgctgttatg ttgagtggag
     4621 ataaaactgc cgttgtacaa aaagtagcgt catctattca aattgctcat gcctttggcg
     4681 atttacttcc agccgataaa gtggaaaaag ttaaaaacct tattcagcaa ggaaaaaaag
     4741 tagcatttgt tggtgacgga ctcaacgatg cgccagttat tgcattgagc gatgttggca
     4801 ttgccatggg tggattggga agcgatgttt ctattgaagc agccgatgta attattcaag
     4861 acgatcagcc ttccaaaata cccgttgcca ttcaaatagg taaagcaaca aaacgcatcg
     4921 tttggcaaaa cattgccttt gcatttggtg taaaactcat cgttttgctg ttgggtgctg
     4981 gcggaattgc tactatgtgg gaagcggttt ttgcagatgt tggtgttgcc ctgcttgcca
     5041 ttttaaatgc tgttcgtatt cagcgaatga cgttttagaa aacaagatta tcaacattat
     5101 tacataaaaa agcaaccgct caagatgaaa agtggttgct tttgttttat tgttatttgt
     5161 tgatagtgtg ctattgaaga gtaactttta gcgttttttg gttgttttct gattgaacat
     5221 taataaaata cacccttgca ggatggttgg ataaatcaaa attcacaaaa tttgtttctg
     5281 ctacagttga tgaaagttgc gttccaagtg ctgaataggc tgtaacaatt gctccttttt
     5341 gaacattgaa aacagatatt tgtccgttgg ttggattggg agaaaccact acttcatctt
     5401 tgttgacgca attggcagaa atggtgcgta aaatttcttg tgttccatca aaatctacct
     5461 gtgttaaacg gtaataggtt gttgagttag attgttgacg gtggttaaag ctgtattttt
     5521 gttcgatggt tgttgaacca tttccatcta tcctacccac ttctgtccaa gttgtcccat
     5581 cttctgaata atcaactaaa aaataatcgt tgtttttttc tgatgctgtt tcccaatcaa
     5641 gtacaaattg gtttccctcg catgtggcag aaaagtcttt taaagttaca gccaacggat
     5701 atgctccaca aaatgtattg gaaactcctg taaaaattgc aaggttaaat tgtctattgg
     5761 aagagacacc tgtggcaatt gcccaagtac ctccggcacc tacttttcct ggaaatacac
     5821 cgcttcccgc atcatttgtc ctatcaaacc aaggtttagc gctatcccaa tgacctcctt
     5881 ggttttcaaa caatacttct aagttagagg taccattgta aacaaacggt gttgaaaatg
     5941 taatggtaat tgtagtacca tttgtacctg ggtaaacaac acttccgtta tatacttgtg
     6001 taaatccccc agtaccagga tagcctggac ttgatgcata attggttaca gcagtatgtc
     6061 taagataaac ataaatatta ttataagttc cacctgcacc aaaagtgttg tccaacctat
     6121 aagaaattga attaattgtt tgtgcaccac ctatttggct gggcatatac aatcctgcag
     6181 accagctata actagaaccc gaccatctat cttgtgtctc aaaaggaaac tcaggggtgt
     6241 tgtatgggca atttacaact tggctaaaca actgtttaga aaaagcgaaa gagaaattag
     6301 cacaaaaagc tatgtagtag tattttttca tcgtgtgttg attaaagtga aaagtattaa
     6361 cacagcgaat ttattaaaat attttctttg agctattgtg ttttaaaaat ctaacacaat
     6421 aatttataac aatatgaaaa tcaaacaact aaaacggtta atcgcaaatt cttcccttgc
     6481 tttttccgtg ctctaagtgt tctaacgctt cggattcttg ttgcaattca acactaacag
     6541 gtttgtttct gagttgctca agattgggtt gtccagcatc tacccatgcg gtgtaccaaa
     6601 tagaacccgt tgcaatgatg gcagccttca ttcttcgctc caccattcca ttcattcgct
     6661 ggtgataggc ttgtgaaaat tcatacgaat aaacctttgt tgtaatattg ccccgttgct
     6721 cgtagctata ttttttgtcc tcaccaaatt cttgtgttaa ctctttttca attctaaaaa
     6781 cagaatcaac tgaacggtgc gaagcaagca caacatccca tgcaaaatca agagggtttt
     6841 taatgtattt tacttttcca acaaagtaat cgtaatcgtc tgcattgatt tccaccaaac
     6901 gactttccca caaaccatga attccttttt gattggtcat ttgcccgttg taattctcgg
     6961 ttgtatgcaa aggcacgtgt gcatcactca tgtaatgacc taaatctgca cttattctca
     7021 aaatattatc cacatttcct tcctcaaacg ctttttgcaa gctgtatttg tatcgaacaa
     7081 taacccaagg cacaatacca taagcctgca aggtatcttc ggtatatttt gctacagcat
     7141 catcccatcg tcggggcatt gctttggaag gatcgtcgcc cggttggtaa taatggtcta
     7201 aatcaatgaa atgacgttgt gcctcccctt ctacggcata cctacggcgg tctgggttgg
     7261 gcgcgttttc ggttacgtat tcaatatgat ctttgtaaaa cccaaacatg ggtggtggca
     7321 aagtgaaaac cgccatacga ttgatgcgtt tatgcccata aaacccccat ctttttgcac
     7381 gcaaagttgg ttcctccact aaggaaagag agagtagaag tagcggcaat acaactacta
     7441 aaaacttttt atagttgaac caatttacca tctttgagta tcggaatcac gtttgtttga
     7501 ttgggtgtta aacaatattt tacatcttca ttcaagttga ggtttttaag ccttcttctg
     7561 tgagaactag attttaaata accaaaataa ttgttttttg ccgaaatgta taaatattta
     7621 gctgccaccg aagagtcttc atccgaagtg aagtttcctg ttgccaacaa atattctgaa
     7681 attgccccgg cacaaatggt atcttctaaa ttgaatttat cttgccaacc cgagcacaaa
     7741 caaagaatgt ttttatcctg tgttgctaaa aactgacaca aagcatctaa atttaaaaaa
     7801 gaaccaacca ataccatctc ggcatctttg gcaatgttta aggctttagt tccattggtg
     7861 gttgaaagca caacttcttg tcctttaaaa tcttcacgca tgtaggaata aggcgagtta
     7921 ccaaagtcaa aaccttccac aatttgcccg ttgcgttctg caccaaccaa ataacttttt
     7981 tctttgtatg ctaaggcttc ttctatggtt ggaacgggaa tgatgg
//
LOCUS       DAOQVQ010000055         7964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10031_length_7964_cov_3.846378, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000055 DAOQVQ010000000
VERSION     DAOQVQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10031_length_7964_cov_3.846378"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKN22_02915"
     CDS             complement(<1..408)
                     /locus_tag="PKN22_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNS41686.1"
                     /translation="MSYFAHETAVIDEGCTIGEGTKIWHFSHIMPNCTIGQKCNIGQN
                     VVISPEVILGNNVKIQNNVSIYTGVICEDDVFLGPSMVFTNVTNPRSAVNRRDQYAKT
                     TVKKGASIGANATIVCGHDIGEYAFIGAGAVVTK"
     gene            complement(413..1408)
                     /locus_tag="PKN22_02920"
     CDS             complement(413..1408)
                     /locus_tag="PKN22_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS41687.1"
                     /translation="MSERKRILITGAAGFLGSHLCDRFVKEDYHVIAMDNLITGRLKN
                     IEHLFKLENFEFYNHDVSKFIHVPGKLDYILHFASPASPIDYLKIPIQTLKVGSLGIH
                     NCLGLAKAKNARVLIASTSEVYGDPTVHPQPEEYWGNVNPVGPRGVYDEAKRFQEAMT
                     MAYHNFHGVETRIVRIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTVFGDGSQTRSFC
                     FVDDLVEGIFRLLHSDYALPVNIGNPDEISIADFAEEIIKLTGTNQKVIYKDLPVDDP
                     KQRQPDITKAKEILGWEPKVSRSEGLKITYDYFKSLSAQELNEVDHYNFDKYIIR"
     gene            1595..4639
                     /locus_tag="PKN22_02925"
     CDS             1595..4639
                     /locus_tag="PKN22_02925"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNS41688.1"
                     /translation="MKKFLLTLLSVISFTTNGFNQENLIPVFSQVYNTVPSIPKGVLE
                     AVAWSKTRYNNINETYAPSCVGMPLPYGVMGVFENGMGAFRNNGVLIEQLSEISVEAQ
                     KENVQLQVLAYAKAFNIIYSQSNINSEAKKVYHTLRTLSSIPDSDSLELYLQDVEIYE
                     IMRFMTDDNFAEQNNFQVKNYSLPTVFGNTNYQVLSASKVIITANNILSNTGISYQTL
                     NSINNFSTKSVDYGPALTDLVPSCNYTSGRSATISAVTIHTMQGSYSGSINWFKNCSS
                     NVSAHYCLRSSDGQITQCVLEANTAWHVGINNSFTIGLEHEGYVSDASWYTIAMYDAS
                     ALLVKDICTSHSIEPKRTAFFPWASTTNYNASSIPGSCVKIKGHQHFPSQTHTDPGAN
                     WNWDYYYKKINEGTPTINYLTNATGNFYDSGGASANYSDDERSIWCIKPANATSVTLN
                     FSSFDIENTWDYLYIYDGEGVYAPLIGIYTGTTSPGTVTASSGIMTIEFRSECATTAT
                     GWNANWTAVINTPQPSDVTAPTTAIVPINDWKTTDFTASFTDNDEVGGSGLEKSFYSV
                     AYKDNNQWTANATRGFYRDDFNSTNINPQFTTVSGNWAISSSSTLEQTDENLFNTNIY
                     TACAQNLSNRYLYAWKGKIGGTGTNRRGGIHLFCSDSIATNRGDSYLIYFRTDGNADV
                     NQNNKVQIYKATNDVLSQKINTDYTINPNQWYDFKVIYDRITGKITVYIDNVLVASWT
                     DPTPIASGNYISFRNGNSTYEVDDFEVFRSRYPTTTVLVGSNSSNEIRTQNNGALNPA
                     GCIRSIVVDSAGNLSSIATQLVNVDWTVPSSLIINDGTGADIDTFSTPTIDANWASND
                     FHSDIASFEIAIGTTSGATDVLNWTNAGLTSSYAYLLSNPTENQLYFVSVKAINNAGL
                     DTIATSNGQRYVTQPSNASLFANEAQQLIVYPNPVDNQLRIENLTETATLLLFDITGK
                     LVIQTVETEKNAVINTENLTPGMYTLIIRSSRGNTIKKITKK"
     gene            4636..5253
                     /locus_tag="PKN22_02930"
     CDS             4636..5253
                     /locus_tag="PKN22_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41689.1"
                     /translation="MMKVLALISVLTLLVSCQPQNSKDITSTVIEKTEQTEETPTFAS
                     EIEFAHYCIDCLRYEKFSALESFIADSVLFSIYGLLDKNSSIKLSFKDIENPSNQPIY
                     WGIHPAKGDSIFHTKDNFFTTYLSAFTSDAPPTKKTVFKGENPPNRDSKQVDYVKYFP
                     TSAYVDFYIAPSKEGYMDWDELIIVVKKVNEKYQLQGVVHNQWMP"
     gene            5382..7964
                     /gene="alaS"
                     /locus_tag="PKN22_02935"
                     /pseudo
     CDS             5382..7964
                     /gene="alaS"
                     /locus_tag="PKN22_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987609.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
BASE COUNT         2644 a         1488 c         1461 g         2371 t
ORIGIN      
        1 tttggtaaca acagcacctg cgccaataaa tgcatattct cctatatcat gaccgcatac
       61 aatggtagca ttagcgccaa tactagcccc ttttttaacc gttgttttag catattggtc
      121 tctacgattt accgcacttc ttgggttagt tacgttggta aaaaccatag aaggtcctaa
      181 aaacacatcg tcttcgcaga taacacctgt gtagatagaa acattgtttt gaatttttac
      241 gttatttcct aaaatcactt caggagaaat cacaacgttt tggccaatgt tgcatttttg
      301 tccgatggtg caattgggca taatgtgcga aaaatgccaa atttttgttc cctctcctat
      361 tgtgcagcct tcatcaatta ctgctgtttc gtgtgcaaaa taactcatag tgctatcgta
      421 taatgtattt atcaaaatta taatggtcta cctcgttgag ctcttgtgca ctcaaacttt
      481 taaaataatc gtaagtgatt ttcaatcctt cactacgaga tacttttggt tcccaaccta
      541 gtatttcttt agcttttgta atatctggtt gacgttgttt tggatcatca actggtaaat
      601 ctttgtaaat cactttttga ttggttcctg tcaatttgat gatttcttct gcaaaatctg
      661 caatggaaat ttcgtcagga tttccaatat taacaggtaa tgcgtaatcc gaatgcaaca
      721 aacggaaaat accttccacc aaatcatcta caaaacaaaa tgaacgtgtt tgtgaaccat
      781 ctccaaaaac agttaaatct tcgccacgca acgcttgacc aataaatgca ggtaacacac
      841 gtccatcatt gagacgcata cgcggtccgt aggtattaaa aatacgaaca atgcgcgttt
      901 ctactccatg aaaattgtga taagccatcg tcattgcttc ttgaaaacgt ttggcttcat
      961 cgtacactcc acgcggccca actgggttta catttcccca atattcttcg ggttgtggat
     1021 gcacagtagg atcaccataa acttcggaag ttgaagcaat taaaaccctt gcattttttg
     1081 cttttgctaa tcctaaacaa ttgtgaatac ccaacgaacc aacttttagt gtctgaatag
     1141 gaattttcaa atagtcaata ggacttgctg gcgatgcaaa atgaagaatg tagtctaatt
     1201 ttcctggtac atggataaac ttggaaacat cgtggttgta aaattcaaaa ttctccaact
     1261 taaaaagatg ctcaatgttt tttagtcgcc ctgtaattag attatccatc gcaattacgt
     1321 gataatcttc tttcacaaaa cgatcacaca aatgcgagcc caaaaatcct gctgctccag
     1381 tgattaaaat tcgttttctt tctgacatgg taaaatgctc tttaatcaag caattattta
     1441 ggtttataac aagctacaaa tttaattagt tttatcaatt aggaaagaaa aagcctgttt
     1501 tttacattgc attttgcaaa taaaaaatca actaaacctt gatttcatcc aaaataatcg
     1561 ttattattgc ctattattta ctactggtat ttttatgaaa aaatttctac ttacactact
     1621 ttcggttatt tcctttacca caaatggatt taatcaagaa aatttaatcc ctgttttctc
     1681 gcaagtttac aacactgttc cttcaattcc aaaaggtgtg cttgaagctg ttgcatggag
     1741 taaaacacgc tacaacaaca tcaacgaaac gtatgcacca tcttgtgtag gcatgccttt
     1801 gccttatggt gttatgggtg tttttgagaa tggaatgggt gcttttcgca acaatggagt
     1861 gttgattgaa caactttctg aaatttctgt agaggcacaa aaagagaatg tgcaattgca
     1921 agttttagct tacgccaaag catttaacat tatctattcg caaagtaaca ttaattctga
     1981 agcaaaaaaa gtataccata cactgcgtac actttcatct attcctgata gcgattcgtt
     2041 ggaattgtat ttacaagatg ttgaaatcta tgaaattatg cgctttatga ccgatgataa
     2101 ttttgctgaa caaaataatt ttcaagtaaa aaactattca ctcccaactg tttttggaaa
     2161 caccaactac caagtattgt ctgcgtcaaa ggtgataatt actgccaata atattctatc
     2221 caacactgga attagctacc aaacactcaa ttcgattaat aatttctcca ccaaatctgt
     2281 tgattacggt cctgccctta ccgacttagt tccatcttgt aattacacct ctggacgaag
     2341 tgctactatt tcagcggtta ccattcacac catgcaagga agttattcag gctctatcaa
     2401 ttggtttaaa aactgtagct ctaatgtttc cgcacattat tgtttgcgct catcggatgg
     2461 acaaattaca caatgtgttt tggaagcaaa tacagcttgg catgtaggaa ttaataattc
     2521 ttttactatt ggattggaac acgaaggata tgttagcgat gcaagttggt acaccattgc
     2581 tatgtatgat gcctctgctt tgttggtaaa agatatttgt accagccatt ctattgaacc
     2641 caaaagaaca gcttttttcc cttgggcaag caccactaat tataacgcct cgagtatccc
     2701 tggttcatgc gtaaaaatta agggacacca acattttcct agtcaaacac acaccgaccc
     2761 aggagccaat tggaattggg attattacta caaaaaaatc aatgaaggca cacccaccat
     2821 caactattta accaatgcaa ctggtaattt ttatgacagt ggaggggcaa gtgccaacta
     2881 ttctgacgat gaacgaagta tttggtgcat caaaccagcc aatgcaacta gtgttaccct
     2941 taatttttca tcttttgata ttgaaaacac ctgggattat ttgtacatct atgatggaga
     3001 aggtgtttat gctccattaa ttggtattta cacggggaca actagtccgg gaaccgtaac
     3061 tgcctcaagt gggattatga ccattgaatt tcgctctgaa tgtgcaacaa cagcaacagg
     3121 ttggaacgcc aattggactg ctgtcatcaa tacacctcag ccttcagatg ttactgctcc
     3181 aacaactgca attgttccaa taaacgattg gaaaacaacc gattttacgg catcttttac
     3241 agataatgat gaagttggtg gaagtggttt ggaaaaaagt ttttattctg ttgcgtataa
     3301 agacaacaat cagtggacgg ccaatgcaac acgtggattt tatcgggatg attttaacag
     3361 cacaaatatc aacccacaat ttactactgt gagcggcaat tgggccatta gctcctcttc
     3421 caccttagaa caaacggatg aaaacctctt caataccaac atttacactg cttgtgctca
     3481 aaatctttct aatcgatact tgtatgcttg gaaaggaaaa attggcggaa caggcaccaa
     3541 tcgccgtggt ggaatacacc tattttgtag cgattctatt gccacaaaca gaggagactc
     3601 atatttgata tactttagaa cagatggaaa tgctgacgta aaccagaaca acaaagttca
     3661 aatttacaag gctacaaacg atgttctctc ccaaaaaatt aatactgatt ataccatcaa
     3721 ccctaatcaa tggtatgatt tcaaagtaat ttacgatcga atcacaggaa aaataacggt
     3781 ttatattgac aatgtgttgg tggcaagttg gaccgatcct acgcctattg caagtggtaa
     3841 ctacatttca tttagaaacg gtaattcaac ctacgaagta gatgattttg aagtttttag
     3901 aagtcgctac cctactacaa ctgttttagt aggaagcaat tcatccaacg aaattcgtac
     3961 acaaaacaat ggagctttga atcctgctgg ctgtatccgt tcgatagttg tggatagtgc
     4021 gggcaatctt tcttccatcg ctacccaact ggtgaatgta gattggacag tcccttcaag
     4081 tttaataatc aacgatggaa caggtgctga tattgacact ttttctacac caaccattga
     4141 tgctaattgg gcatcgaatg attttcattc agacattgcg tcgtttgaaa tagctattgg
     4201 tactacaagt ggcgccaccg atgtattaaa ctggacaaac gcagggttaa catcttccta
     4261 cgcttattta ttgagcaatc caactgaaaa tcaactttat tttgtttctg taaaagcaat
     4321 caacaatgct ggacttgaca ccatagccac tagtaatgga caacgttatg ttactcaacc
     4381 tagcaatgct tcgttatttg caaatgaagc gcaacaatta atcgtttacc ccaatcctgt
     4441 tgacaatcaa ttgcgcattg agaatttgac ggaaacagcc actcttctac tttttgacat
     4501 aactggaaaa ttagtcattc aaacagttga aacagaaaaa aatgcagtca tcaacactga
     4561 aaatttaacc cctggaatgt acacactaat tattagatca agtcgtggaa acactattaa
     4621 aaaaatcact aaaaaatgat gaaagtatta gcattaattt cagttcttac gttgttggtt
     4681 agctgccaac cccaaaatag taaagatatt acatcaacag taattgaaaa aacagaacaa
     4741 acggaagaaa caccaacatt tgccagcgaa attgaatttg cacactattg cattgattgc
     4801 ttgcgctatg aaaaatttag tgcattagaa tcgtttattg cagattcggt gcttttttct
     4861 atctatggtt tacttgataa aaacagcagt atcaaactca gttttaaaga tatagaaaac
     4921 ccttccaatc aaccaattta ttggggcata cacccggcaa aaggggattc catttttcat
     4981 acaaaagata attttttcac aacctatctt tctgcattta caagtgatgc tccaccaact
     5041 aaaaaaaccg tttttaaggg cgaaaatcca cccaatagag actccaagca ggtagattac
     5101 gtaaaatatt ttccaacttc agcgtatgtt gatttttaca ttgcgccctc aaaagaagga
     5161 tatatggatt gggatgaatt gattattgtg gtaaaaaaag taaacgaaaa ataccaactt
     5221 caaggtgtag tacacaacca atggatgccc taaaaagtga tttctatcta cttttggcaa
     5281 cattaaaccc aaaaaaacag aaatattcct ttatttttta tagttaaatg tgcaaataat
     5341 caactaaatt tgcgcttcta attaaataaa cgcattgatt tatgaatgtt tctgaaatac
     5401 gttcgcaatt tttaagtttt tttgaatcca aagggcataa aatcgttcct tcggcgccaa
     5461 tggttgtaaa aaacgaccct actttgatgt ttaccaatgc ggggatgaat cagtttaaag
     5521 atttcatttt aggcaatgcg acaccagagt ttagaagagt cgccaattct caaaaatgct
     5581 tgcgcgtatc aggaaaacac aacgatttgg aagaagtagg agttgacacc tatcaccaca
     5641 ccatgtttga aatgttgggt aactggtcgt ttggcgatta tttcaaagaa gaagccattg
     5701 gttgggcatg ggaactttta acagaagtat acaaattgcc aaaagaccgc ttgtatgtta
     5761 ccgtattcca aggtgatgaa caagacaaaa caccaatgga tgccgatagt tttgctattt
     5821 ggaaaaaata cattgcagaa gaccgaattt tgttggcttc taaaaaagat aatttctggg
     5881 aaatgggtga cacaggacct tgtggaccgt gtacggaaat ccatgtggat attcgcccag
     5941 aatctgaaag agcaaaaata gatggtaaat cattggtaaa caacgaccat ccacaagtta
     6001 ttgaaatttg gaacaacgta tttatggaat ttaatcgcat ggcaaatgga tcattggtgc
     6061 cacttcctga aaaacacgtt gatacaggta tgggattgga acgtttatcc atggcattgc
     6121 aaggcaaata ttctaactac gacaccgatt tatttcaatc gttgattcaa aaaatggttg
     6181 cactttcggg taaaaattat ggtacggacg aggcaacaga tattgcattg cgtgtcattg
     6241 cagaccacgt gcgtgcgatt tcgttttcaa ttaccgacgg acaattgcca tccaacacag
     6301 gtgctggtta tgtaattcgt agaattttac gccgtgctgt acgttacgga taccaaacat
     6361 taaatttgaa agaaccgttt ttgtgcgaac tttctcaaac gttaaccgaa gtgatgggaa
     6421 cagcctatcc agaattaaac gcgcaaaaaa gtttggttta caatgtaatt aaagaagaag
     6481 aacaaagttt ttttcgcaca ttggagcaag gaatcaaacg tatagaagta ttgattcaat
     6541 cggtaaaagc taaaaaccaa acagttattg caggcgaaga tgtgtttgag ttgtacgata
     6601 cttttggttt tccgttagac ttaacttcat tgattgctcg tgaaaatcag ttagaaattg
     6661 acgaaaaagg gtttgagatt gagttgaaca agcaaaaaga tcgttctcgt gcggcaacag
     6721 ccattgaaac agatgactgg gtgatactta aaaacaatac ggttgaagaa tttattggat
     6781 acgataacac cactgcaacg gttgaattgg taaaataccg caaagttgtt caaaaacaaa
     6841 aagcatttta tcagttggta tttgaccaaa cgccatttta tccagaagga ggtggacaag
     6901 ttggagacta aggatacatt gagcaaaacg gtgaaaaaat ctacattttc gatacgaaaa
     6961 aagaaaacaa tttgattgtt catttttcag agaaaattcc aacaacagtt tcgggttctt
     7021 tccaagcagt tgtgaacatg gaaaaacgtg ctgcttcaat gaaaaaccac tctgctacgc
     7081 atttattgca ccatgcattg cgtaaagtat tgggaacaca cgttgaacaa aaaggatcgt
     7141 tagttagtcc atctaatttg cgtttcgact tttcgcattt ttcaaaagta accgacgagc
     7201 aattagcaga gattgaagct attgtatcac aagaaatcaa aaaaggaatt gcgttagaag
     7261 agtttagaaa tacacctatc gaaaaagcaa aagcaatggg tgctatggcc ttatttggag
     7321 aaaaatatgg cgatgctgtg cgtgtgatta aatttggaga atcaattgag ttgtgcggag
     7381 gaacgcacgt tgcttctaca agtgcaattg gtttatttaa aatcacaact gaaacagctg
     7441 ttgctgctgg tattcgccgt attgaagcta ttacaggaga tgttgccgag cagtattaca
     7501 aagaaagtgc tgaaaagttg gaagccattg caaccatctt caaaaatcct aaagaccttg
     7561 taaaagcagt gaatgattta caagctaaaa atttggattt acaaaaagaa attgaagcat
     7621 tgcaaaaaga acgtgcaatg gcagtaaaac acgaattaaa aaataaaatt gaaaccatta
     7681 atggtgttcc attcttagga gaggttgtat cactggatgc aggttcaatt aaagacatct
     7741 tatttcaatt aaaaggtgaa tacccaagtt ttgtaggtgt aattggtgga aaaacagatg
     7801 ataaatgctc gttaaacatc attttaaccg acgacataat ccaaaacaaa gggttaaatg
     7861 ccggcagttt aatcaaagaa acagccactt taatcaatgg aggcggtggt ggacaaccat
     7921 tttttgctac tgcaggaggt aaaaatccca acggacttaa aaac
//
LOCUS       DAOQVQ010000056         7884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10164_length_7884_cov_4.150977, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000056 DAOQVQ010000000
VERSION     DAOQVQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10164_length_7884_cov_4.150977"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKN22_02940"
     CDS             <1..362
                     /locus_tag="PKN22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41690.1"
                     /translation="RARNLHATAIAVATQYEGKFPTTYAEIIQLKGVGAYTAAAIASF
                     AFNEPKAVVDGNVIGCWRAILILLLPSIQRKEKKSFSSWPMNYCPTQKMPSITKQLWN
                     LVPCNVYRLTHRAIHVC"
     gene            302..787
                     /locus_tag="PKN22_02945"
     CDS             302..787
                     /locus_tag="PKN22_02945"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNS41691.1"
                     /translation="MEFGALQCVPVNPSCDTCVLNNNCQAYLNKTVNQRPVKTKKVKV
                     RNRYFYYAIFESENNTCIQKREQNDIWQHLYEFPLFETTTSLTDEELLEYYYRNYQLK
                     PAYISTAIKHVLSHQRIITRFLHFSEIPQKLQAFVVSKDELVHYPLPRLIEKYLENQS
                     K"
     gene            914..1390
                     /gene="ssb"
                     /locus_tag="PKN22_02950"
     CDS             914..1390
                     /gene="ssb"
                     /locus_tag="PKN22_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNS41692.1"
                     /translation="MASGVNKVILVGNLGKDPEVRRLENGGIVANFSMATTETYFDRN
                     TNERKDITDWHNIVAWRGLAEVIEKYVRKGSKLYVEGKLKTRSWTDKDGNNRYTTEVY
                     ADNIIMLNRAEQAPVINEPIFTNEGTPSPLTPLEGFSAPTGYMPPQQEEETDDLPF"
     gene            1401..2726
                     /gene="gldE"
                     /locus_tag="PKN22_02955"
     CDS             1401..2726
                     /gene="gldE"
                     /locus_tag="PKN22_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNS41693.1"
                     /translation="MNQLDPDPLASVALTAFNAADVLLVGIILLLLIASALFAGAEVA
                     FFSLSELEKDQIKKSEKARAKIAEKLLDKPKRLLATILVGKNVLNVAFVVVSFIVLTK
                     IGVYFHTNWVVLTAINVFLISLFLVFVGESYPKTIAYKNKATYVCLMAYPILVLQYLP
                     PFSWLIKPLSSGTKTLEKRAKRKGIEISPVALETVLSLSQDPEENKNELNILQGVLKF
                     GDTDVRQIMCPRMDVIGIDEKLSLEEVLNVVVDAGYSRLPVYKENFDEIVGVIFVKDL
                     FHSSVKKNDFDWRTLVREPMYVPENKKIDDLLREFQLKKIHIAIVIDEYGGPTGIVTL
                     EDVLEEIVGDITDEYDGDEETFTQINENTYIFEGQTSLADFYKAINIDGNAFEERKED
                     AESIGGFLIEVFGKIPKVNETITIENIKLIVEASDKKRVRKVRVVIEAI"
     gene            complement(2723..3280)
                     /locus_tag="PKN22_02960"
     CDS             complement(2723..3280)
                     /locus_tag="PKN22_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41694.1"
                     /translation="MEQPDFSSILNKRPHLSIRSIENYEKRLRNDEKKRDIEKSEALL
                     SVQQLGFDSAIAYKESGQPFLVNYPHLHLSISHSKGWIAVYVAEKPVGIDIEVENDRI
                     LYGATYYINQHEAHFMDDVKLLHLVWGAKEAFYKLKEGKIANSKEDITIKSINFSTQQ
                     IEVVFEATSYFFDFLQEGKITVVLN"
     gene            3387..4544
                     /locus_tag="PKN22_02965"
     CDS             3387..4544
                     /locus_tag="PKN22_02965"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNS41695.1"
                     /translation="MNETYLHFIWKLKRLPFHQLTTTDGKLLKILKNGIHNVSESGPD
                     FYNARIFYEEMEWAGQIEMHVKSSDWYKHGHQNDVAYSNVILHVVYEHDRAVFVNNSL
                     LPTVELKNYIDWEHFENWERFASSVKEIPCDDALSNIDSIYLQSMMQKAIVERQTRKI
                     NYLLYTQSQEMNHQAVLYHLIGKAFGTKVNALPFELLTNQLPLSLLKRMDKKQQKQLV
                     LHTSGLFDLQPTDHFSLPPSIWKRKGLRPPAFPEKRIEQFADFIAAIDFELLVSYLSP
                     QEIYTYVVQQAKNQNLSENLINQLFVNAFVPYFWYKSLREENEKMQEQILAFLEELQP
                     DDNHIIKKWKKTGVIVSNAYQSQALIELYNEYCTYKKCLNCQIGVKILKGE"
     gene            4575..4814
                     /locus_tag="PKN22_02970"
     CDS             4575..4814
                     /locus_tag="PKN22_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HNS41696.1"
                     /translation="MRLIKKIVFFFEMRSFGVSQWWARKLGIQTDRVRLGFIYASFFT
                     FGSPLIIYLIMAWILEHKHYFKLQREKPKTSIWDL"
     gene            4818..5714
                     /locus_tag="PKN22_02975"
     CDS             4818..5714
                     /locus_tag="PKN22_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS41697.1"
                     /translation="METTTMKIVITGANGLLGQKIVIQLLKKNVPFLATSTGENRNPD
                     CPADHYVEMDICNKAQVTQVIEQFCPTHVIHTAAMTNVDACELNPEQCHQINVVGTQN
                     VFEATQKVNAHFQLLSTDFVFDGQKGNYTEEDAVNPLSVYAQSKVDAENLLLKGNYSN
                     YSIVRTIIVYGNAHHLSRSNLFLWAKSALPKGEQMNVVDDQFRAPTWANDLAWGCIRI
                     CELNERGIYHLSGPTTFSIFEIVERIARYYNYDTKLLNKISSATLSQPAARPPKTGFD
                     LTKARKNLGYQPKTIEETLDLF"
     gene            5799..6599
                     /locus_tag="PKN22_02980"
     CDS             5799..6599
                     /locus_tag="PKN22_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS41698.1"
                     /translation="MVVLMPRLLQDLSSNEPLLVTFKEIDSGISEIEKKKYLSVPYYK
                     KKSIKYKSPPTKFDPNKYQEHEWIALGLSPKQAKVVLSFAKRGIKNNDELKQIFVIDD
                     RLFQLIKDSTIYPVYTLKQGVDNKNIYSQKKQPIELNKATEEQLVALPMIGDFLAKKI
                     IERRQQLGGYHSLEQLLEIKFLDVSKLDVIRPFLTIDFNEVKLININTCTVEDLQKHP
                     YISWNVANSIIKMRVYLKKYTNFDQLLESRLISIELLEKITPYLTLED"
     gene            6611..7141
                     /locus_tag="PKN22_02985"
     CDS             6611..7141
                     /locus_tag="PKN22_02985"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNS41699.1"
                     /translation="MTIENVLKAAIRDVPDFPQPGVIFKDITPIMLNPELSEQVLNHL
                     VETYKNKSIDVVAGIESRGFLFGFPLAMKLGVPFVLIRKKGKLPYEKISHDYQLEYGT
                     STIEMHTDAIKKGQKVLIHDDLLATGGSAEAAAHLIQKCGGEVVGFNFLVGLDFLEGK
                     SKLNNISHNIYSLVTY"
     gene            7191..>7884
                     /locus_tag="PKN22_02990"
     CDS             7191..>7884
                     /locus_tag="PKN22_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS41700.1"
                     /translation="MDFGLNENQKMIAQMVRDFTEKEVKPYLNKWDDEEYFPVETMKK
                     LGELGLLGIYIPEEYGGSGFSYNEYVTALIELGKVCGGLGLSVAAHNSLCTGHIYYHG
                     SEEQKRKYLPKLASGEFIGAWGLTEPNTGSDAMRMKTTAVKDGNDWIINGAKNWITHG
                     LSGDVAVVLVRTGELLDSRGITAFIVEKGTPGFSAVKIKGKLGVRASETAELIFDNVR
                     VPQENVIGEVGMG"
BASE COUNT         2711 a         1304 c         1467 g         2402 t
ORIGIN      
        1 gtagagcacg taacttgcac gcaaccgcta ttgcagtggc aactcaatat gaaggtaaat
       61 ttccaacaac gtatgctgaa atcatacaac ttaaaggtgt tggagcatac actgctgcgg
      121 caattgcaag ttttgcattc aacgaaccca aagcagttgt ggatggtaat gttatcgggt
      181 gttggcgcgc tattttgata cttctactcc catcgattca acgcaaggaa aaaaagagtt
      241 tcagcagttg gccgatgaat tattgcccca cccaaaaaat gccgtctata accaagcaat
      301 tatggaattt ggtgccttgc aatgtgtacc ggttaaccca tcgtgcgata catgtgtgct
      361 gaacaacaat tgccaagctt atttgaataa aacggtcaat cagcgaccag taaaaaccaa
      421 aaaagtaaaa gttagaaacc gttattttta ctacgccatt tttgagtcag aaaacaatac
      481 ctgcattcaa aagcgggaac aaaacgatat ttggcaacat ttgtatgagt ttcctctctt
      541 tgaaaccact acttcgttga ccgatgaaga gctgttagaa tattattatc gcaattatca
      601 attaaaacca gcgtatatat ccacagcaat taaacatgtt ttatcacatc aacgcatcat
      661 cacccgtttt ttacattttt cagaaatccc tcaaaaatta caagcatttg ttgtttcaaa
      721 agacgaattg gtgcattatc ccttgccacg attaattgaa aaataccttg aaaatcaatc
      781 gaaataatta gttgttttgt gctttcaaaa tttcacgcac gtttaaaatt atcttcaatt
      841 caacaaaaaa ttcaaaaatt ttatatattt gtgtataacc ttgttgtaaa agacattaaa
      901 acaatacatt aatttggcat ctggagtaaa taaagtcatc cttgttggga atttaggaaa
      961 agaccccgaa gtaagaagat tggaaaacgg tggaatagtt gcaaattttt caatggcaac
     1021 cacagaaacc tattttgata gaaataccaa cgaacgtaaa gacataaccg attggcacaa
     1081 cattgtggca tggagaggtt tggcagaagt gattgagaaa tacgtacgaa aaggctctaa
     1141 attgtatgtg gaaggtaagt taaaaacacg ttcgtggacc gataaagatg gcaacaaccg
     1201 ttatacaaca gaagtatatg ccgacaacat tatcatgctc aaccgtgcag aacaagcacc
     1261 agtgattaat gagccaattt tcaccaacga aggtacgcca tcaccgctaa cacctttgga
     1321 aggtttttca gcgccaacag gctatatgcc accacaacaa gaagaagaaa cagacgattt
     1381 accattttaa ataaagaata atgaatcaat tagatcctga ccccctggcc agtgttgcac
     1441 tcacagcgtt taatgctgct gatgtgcttt tagtaggaat cattctatta ctactcattg
     1501 cctctgcgct ttttgctggt gcagaagttg ctttcttttc actcagtgaa ttggaaaaag
     1561 atcaaatcaa aaaaagtgaa aaagcaaggg caaaaattgc tgaaaaatta ttggataagc
     1621 ctaaacgttt attggccacc atcttagttg gaaaaaatgt gttgaacgtt gcatttgtgg
     1681 tggtttcttt tattgttctc acaaaaattg gtgtttattt tcatactaat tgggtggtgt
     1741 tgactgctat caatgttttt ctaatatcac tgtttttagt gtttgtgggt gaatcgtatc
     1801 caaaaacaat tgcctataaa aacaaagcaa cctatgtgtg tttaatggcc tatcccattc
     1861 ttgttttgca ataccttccg cctttttcat ggttgattaa accattgtcg agtggaacca
     1921 aaacattgga aaaacgagcg aaacgcaaag gaatagaaat ttctcccgtt gcattggaaa
     1981 ccgtgctttc gttgtcacaa gaccctgaag aaaataaaaa cgaactcaat attttgcaag
     2041 gagtactcaa atttggtgat accgatgtgc gccaaatcat gtgtccgcgc atggatgtta
     2101 ttggaattga tgaaaaacta tcgttggaag aagtcttaaa cgttgtggtg gatgctggct
     2161 attcgcgttt accggtttac aaagaaaatt ttgacgaaat cgtaggcgtc atttttgtta
     2221 aagatttatt tcattcatcg gttaagaaaa atgattttga ttggcgaaca ttggtgcgag
     2281 aaccgatgta tgttccagag aataaaaaaa ttgacgattt gctacgtgag tttcaattga
     2341 agaaaattca cattgccatt gttattgatg aatatggagg tccaacagga attgtaaccc
     2401 tagaagacgt gttggaagaa attgtgggcg atattaccga tgaatacgat ggtgacgagg
     2461 aaacatttac tcaaattaat gaaaacacct atatttttga gggacaaaca tcgcttgccg
     2521 atttctacaa agcaatcaac attgatggaa atgcttttga agaacgaaaa gaagatgccg
     2581 aatccattgg tggattttta atcgaagttt ttggtaaaat tcctaaagta aacgaaacca
     2641 ttaccataga aaacatcaaa ttgattgtgg aagcatcgga taaaaaacgt gtgcgaaaag
     2701 tgcgtgttgt aattgaagct atttaattca gcaccactgt aatttttcct tcttgcaaaa
     2761 agtcaaaaaa gtatgaggtt gcttcaaaaa ccacttctat ttgttgggta gaaaaattga
     2821 tggatttgat agtaatatct tcttttgaat ttgcaatctt gccttctttt aatttgtaaa
     2881 acgcttcttt agcaccccaa actaagtgca acaattttac atcgtccata aaatgcgctt
     2941 cgtgttggtt gatgtaatag gtggcaccat acaaaatgcg atcgttttct acttcaatgt
     3001 caataccaac tggtttttca gctacataaa cagctatcca acctttggaa tgtgaaatag
     3061 aaaggtgcaa atgcggataa ttgactaaaa aaggctgacc actttctttg taggcaatag
     3121 ccgaatcaaa tcctaattgt tgcaccgata aaagggcttc gcttttttca atgtcgcgtt
     3181 ttttttcatc gttacgcaag cgtttttcgt aattttcaat tgaacgtatc gataaatgcg
     3241 gtcttttgtt aagaattgat gaaaagtctg gctgctccat tttgtttgtt ttttgaacga
     3301 ataggctaat ttaaggataa aaaactcaaa tacatttttt attctcccaa cttttttgta
     3361 atttcattca acctatcttt taaattatga atgagaccta tttacacttt atttggaagt
     3421 tgaagcgttt accgtttcac caactaacaa caaccgacgg aaaattattg aaaattctga
     3481 aaaatggaat tcacaatgtg tccgaatcgg gccctgattt ttataatgca cgtatttttt
     3541 acgaagaaat ggaatgggcc ggacaaattg aaatgcatgt aaaaagttct gattggtaca
     3601 aacacggtca ccaaaacgat gttgcctact ccaatgtgat tttacacgtg gtgtatgaac
     3661 acgatagagc cgtttttgta aacaacagtt tattgccaac cgtagaatta aaaaactaca
     3721 tcgattggga gcattttgaa aattgggaac gctttgcgag ttcggttaaa gaaattccgt
     3781 gcgatgatgc gctttccaac attgattcaa tttaccttca atctatgatg caaaaagcca
     3841 ttgttgaacg tcaaacccga aaaatcaact atctgttgta tactcaatct caagaaatga
     3901 accatcaagc ggtgttgtat catttaattg gaaaagcttt tggaacaaaa gtcaatgcac
     3961 tgccttttga gttactcacc aatcaattgc ctttatcgtt gcttaaacgt atggacaaaa
     4021 aacagcaaaa acaacttgtt ctgcacacaa gcggattgtt tgatttgcaa ccaaccgacc
     4081 atttttcact accaccttcg atatggaaac gaaaaggatt gcgcccgccc gcttttccag
     4141 aaaaaagaat agaacaattt gccgatttta tcgctgctat tgattttgaa ttgctcgtta
     4201 gttatctttc tcctcaagaa atttacacgt atgttgtgca acaagcaaaa aatcaaaatt
     4261 tatccgaaaa tttaattaat caattgtttg tcaacgcatt tgttccctat ttttggtata
     4321 aatcacttcg agaagaaaat gaaaaaatgc aagaacaaat acttgccttt ttagaagaat
     4381 tgcaacctga tgataatcac atcattaaaa aatggaaaaa aacaggtgtg attgttagta
     4441 acgcctatca aagtcaggca cttatagagt tgtataatga atattgcacc tacaaaaaat
     4501 gtttaaattg ccagattggt gtaaaaatct taaaaggaga gtaaaaatga ttaattttgc
     4561 ttcaaactcg ttggatgagg ttaattaaaa aaatagtgtt cttttttgaa atgcgctcct
     4621 ttggtgttag tcaatggtgg gcaagaaaat tgggcattca aaccgatagg gttcgattgg
     4681 gttttatcta cgcttctttt tttacgtttg gctctccact tatcatctac ctaattatgg
     4741 cttggatact ggaacacaaa cactatttta aattgcaacg tgaaaaacca aaaacatcta
     4801 tttgggattt gtaaacaatg gaaactacaa caatgaaaat agtgattaca ggtgccaatg
     4861 gtttattggg acaaaaaatt gtaatacagt tgctgaaaaa aaatgtgccc tttcttgcaa
     4921 catcaactgg tgagaacaga aatccagatt gcccagccga ccattatgtt gaaatggata
     4981 tttgcaataa agcacaagta acacaagtta tagaacaatt ttgtcctaca catgtaattc
     5041 acactgccgc aatgactaat gtggacgcct gtgagttgaa tcctgagcaa tgtcatcaaa
     5101 tcaatgtagt tggaacacaa aacgtttttg aagcaacaca aaaggtaaac gctcattttc
     5161 aattgctttc aaccgatttt gtgtttgacg gtcaaaaagg taattacacc gaagaagatg
     5221 ctgtgaatcc gctaagtgtg tatgcgcaat caaaagtgga tgcagaaaat ttactgttaa
     5281 aaggaaatta ttcgaattat tccattgtca gaaccatcat tgtttatggc aatgcccatc
     5341 atttatcgcg ctctaattta tttttgtggg ctaaatcagc tttgccaaaa ggagagcaaa
     5401 tgaatgtggt tgacgaccaa tttcgtgcac ctacttgggc caatgacttg gcttggggat
     5461 gcatccgtat ttgtgagcta aatgaacgtg gaatttatca tttaagtgga ccaaccacct
     5521 tttccatttt tgaaattgtg gaacgcattg ctcgctatta caattacgac acaaagttat
     5581 taaacaaaat ttcaagtgct accttgagcc agcccgctgc acgaccacca aaaaccggat
     5641 ttgatttaac aaaagcaaga aaaaatttag gatatcaacc aaaaactata gaagaaacat
     5701 tagatttgtt ttaaactaat tttttttcat tgaattggcc ccaaaaaata tccaatcgca
     5761 aagcacgagg tgtttttgtg ttgttaatca tcattatcat ggtggtgtta atgccacgat
     5821 tgttacaaga cttatcaagc aatgagcctt tattagtaac gtttaaagaa attgatagtg
     5881 gtatctcaga aattgagaaa aaaaagtatt tatcagttcc atattataag aaaaaatcaa
     5941 taaagtataa atcgccacca acaaagtttg acccgaataa ataccaggag cacgagtgga
     6001 tagctcttgg tctatcacct aaacaagcaa aagtagttct tagttttgca aaaagaggaa
     6061 ttaaaaacaa cgatgaatta aaacaaattt ttgttattga tgatcgtctt tttcaattga
     6121 taaaagattc tactatttat cctgtttata cattaaaaca aggagttgac aataagaata
     6181 tatattctca aaaaaaacaa cctatcgagt taaacaaagc aacagaagag caattagttg
     6241 ccttgccaat gataggagat tttttagcca agaaaattat tgaaagacga cagcagttag
     6301 gaggatatca ttcattagaa caattactag aaattaagtt tttagatgtt agcaagttgg
     6361 atgtcataag acctttttta acgatagatt ttaatgaagt aaaacttata aatatcaata
     6421 cctgcacagt agaagattta caaaaacacc cttacattag ttggaatgtt gcgaattcaa
     6481 ttattaaaat gcgtgtttat ttaaaaaaat atactaattt cgaccaactt ttagaatctc
     6541 gactgatttc tatagagtta cttgaaaaaa tcacaccgta tctaacactc gaagactaga
     6601 ataataattt atgacaatag aaaacgtttt aaaagcggct atccgcgatg ttccagattt
     6661 tccgcaacca ggtgtcatct ttaaagatat aacacctatt atgctgaatc cagaactttc
     6721 tgaacaagtt ttaaaccatt tggttgaaac atataaaaat aaatctattg atgtagttgc
     6781 tggtatagag agtagaggtt ttttattcgg ctttcctttg gctatgaaat taggagttcc
     6841 ttttgtatta attcgcaaaa aaggaaaatt accttatgaa aagattagtc atgattatca
     6901 gttagaatat ggaacttcaa ctatagaaat gcatacggat gccattaaaa aagggcaaaa
     6961 agtattaata cacgacgact tacttgctac tgggggctct gctgaagcag ccgcacattt
     7021 aattcaaaaa tgtggaggtg aagtagttgg atttaatttt ttggttggac tcgacttttt
     7081 agaaggaaaa tcaaaattaa ataacatatc acacaatatt tatagtttag ttacgtatta
     7141 gcgtgtgaga tttataaaaa ttagttaaaa aaattaaaat ttagacagaa atggactttg
     7201 gtttaaatga aaatcaaaaa atgattgcgc agatggtgcg tgattttaca gaaaaagaag
     7261 ttaaacctta tttgaacaaa tgggatgatg aagaatattt tcctgttgaa acaatgaaaa
     7321 aattaggtga acttggtttg ttaggtatct acattcctga agaatatgga ggaagtggat
     7381 tttcatacaa cgaatatgtt actgcactta ttgaattagg aaaagtttgt ggtggattag
     7441 gtttgagcgt agctgcacat aactctttgt gtacaggtca catctactac cacggaagtg
     7501 aagaacaaaa acgtaaatac ttaccaaaat tagcttctgg tgaatttatc ggtgcatggg
     7561 gattaacaga accaaataca ggatctgacg ctatgcgtat gaaaacaact gctgttaaag
     7621 acggtaatga ttggatcatc aatggtgcaa aaaactggat tacacatggt ttatctggtg
     7681 atgtggctgt tgttttggtt agaacaggag aattattgga tagccgtgga attactgcct
     7741 ttatcgtaga aaaaggtaca ccaggttttt ctgcagtaaa aattaaagga aaattggggg
     7801 taagagcatc tgaaactgct gaattgattt ttgataatgt gcgtgttcca caagaaaatg
     7861 taattggtga agttggtatg ggct
//
LOCUS       DAOQVQ010000057         7839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10236_length_7839_cov_4.063078, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000057 DAOQVQ010000000
VERSION     DAOQVQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10236_length_7839_cov_4.063078"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKN22_02995"
     CDS             complement(<1..628)
                     /locus_tag="PKN22_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNS41701.1"
                     /translation="MSKYSRPSNPNENLLVTIKTLFGLENVLKEELIELGYSNIVVLN
                     RAVQLEASWKDVYNMNLNLRCALSILVEIAFFRIRDEQDLYKQAKKIDWTAYFDENKT
                     FAVKGVVMSHLFRHTKFPFLLLKDAIVDVFRDKTGKRPDVNAKNPQVHIDVHINEDKV
                     IISLNSSGLPLYQRGYRDDTGDAPLNEVLAAGMIRLAKWDKKTPLIDPF"
     gene            674..2962
                     /locus_tag="PKN22_03000"
     CDS             674..2962
                     /locus_tag="PKN22_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNS41702.1"
                     /translation="MKAIFRHLFAIVVFTQSFFMLGQEKIGLVLSGGGATGLAHIGVI
                     KALEENGIPIDYISGTSMGALVGGMYAAGYSPEEIEAFAKSDAFLKMATGQIETHQRF
                     AYRESDENAAMFKFGIAIDSSILTSIPTHYRSSAYVDYKLLELYGPVGEMVHNNFDSL
                     FVPYRCVASDIANKKSVVFRSGKLNQAIRASMTYPFYFEAIEVNKTLLFDGGLYNNFP
                     ADVMFNDFNVDYIIGSNVSYNNPKPKRNNIISQISNMLMFYSNYTIPCESGILIQPDI
                     KSTTFEFKDAAEIIAEGYRQAQPYIDSILQHTTRRVSKEELQQRRAAFRNKIIPVQIS
                     SVTTNMRKQKPFSFAKNSMIRRKEEKVNAKVFERRYFRLRATPQIGFLFPTLSLKQDS
                     TYNLDLTVNKAKDFNFEIGGHFSTRAVNTGFIGVSYSHIGKVAAKVYANSYFGKFYTS
                     VKTEVGMDIPSTYPVNISGNFVLNKLDYFRSFATFFAPERPSFLVQNEMYTGLEFKQP
                     IWNSIKSTFGGRYFLLEDRYYQDKNFTNADTTDFTQFQGFTASWELVQNTLNRKQFAT
                     SGHFAALKIRYINGREHSKSGTTAPIPFDNYKYHSWISISGEFQSYPVSLKFFHLGLH
                     GNAVFNSQSLFSNYTASLLSLNAYTPLPDMATYFMSEFRSPQYVGAGLNLVFTIKKVL
                     DIRADGYYYQPFRALVKNDDGTFGYAKPFKGESFVASASIIYHSPVGPLRATFNYFPK
                     QHIPYAFQISFGYVLFNARAIR"
     gene            3041..3490
                     /locus_tag="PKN22_03005"
     CDS             3041..3490
                     /locus_tag="PKN22_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNS41703.1"
                     /translation="MSDINQLIIDSSIRSAWFKLSRMYNQKALEHGLTMSIGFILMNI
                     DKEGTPSTQLGPRMGMQATSLSRTLKTMEEKGLIERKEEGNDKRKVLIFLTKSGVDTR
                     REVRNFLVDFNNKVVENISSSKLKVFFEVIQKIDDLIDSEIQENKNK"
     gene            3499..5904
                     /locus_tag="PKN22_03010"
     CDS             3499..5904
                     /locus_tag="PKN22_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNS41704.1"
                     /translation="MSRIIKKVAVLGSGVMGSRIACHFANIGCEVVLLDIVPRELNDA
                     EKAKGLTLEDKVVRNRIVNDELQATLKSNPSPIYLQSFAQRIKTGNFDDDLKEIASCD
                     WVIEVVVERLDIKKQVFENVEKFRKPGTIISSNTSGIPIHLMLDGRSDDFKAHFLGTH
                     FFNPPRYLQLLEIIPTPETKTEIVDFLMDFGSRYLGKKTVLCKDTPAFIANRVGVYSI
                     MALFHVIKEAGLTVEQVDKLTGPVMGRPKSATFRTCDVVGLDTLVHVANGLKANCPND
                     EEKALFELPDYITKMVENGWIGAKAGQGFYKKVKKEDGSSEIMVLDLNTLEYRSQEKV
                     KFATLEMVKPIEDLKQRTKMLFMGQDAAGKLFNQLFGGLFAYVSNRIPEIADDLYKID
                     DAIKAGFGWELGPFETWDLIGIEQGLKLIAKNNKKAALWVEDMKAAGFNSFYKSEGGK
                     RYFYDIQTKTYQIIPGTENLVDLEALRSEKKVWGNSDVTVVDLGDGILNLEFHTKMNT
                     IGGGVIEGINKAIDLAESNYKGLVISNNGQNFSAGANVGLIFMMAAEQDFDELNFSIK
                     AFQDTMMRVRYSNIPVIVAPHNMALGGGCELSMHSDKVVAHAELYMGLVEFGVGLIPG
                     GGGSKEFAKRFSDDMKKGDIKINRFRDRFLTIGQAKVSTSAYEAFELGYLRPGTDEVV
                     VSRSHQLTRAKQAALEMANKGYIAPKREKNITVVGQEGLGIVYVGANSMTSGKYISEH
                     DQLISEKLGFVLCGGDLSENTVVSEQYLLDLERKAFLELCAEPKTLERLQSLVKFGKI
                     LRN"
     gene            6047..6856
                     /locus_tag="PKN22_03015"
     CDS             6047..6856
                     /locus_tag="PKN22_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41705.1"
                     /translation="MKSIIVSSLLIVALVACKSTTSKEVEQSAATTSNDGPTIAANSQ
                     DDKMAAIDQQLDKLSRIESLNYSKEDGTSISAIAYMASQDTIAKVEVKQYNAKTVTNP
                     KIHFYYDKGLLFASKKLEEKIIDQTAYFSEEVSFYNEKGDVIQSKERISDYEEGLINA
                     AYKKINNVTHDPTEAFLVLKQQGPFAVTFQGFVDNGPYHFLIVGENVAKDGYTAALSI
                     QKEDATIKYLRNEGKKALGKELMVQFVNHMDNQGYEMQILTDLALVERKKK"
     gene            complement(6860..7462)
                     /locus_tag="PKN22_03020"
     CDS             complement(6860..7462)
                     /locus_tag="PKN22_03020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNS41706.1"
                     /translation="MFRSPKILRRLFPLRIWGIAVSEPIVFLTFDDGPNPDITPWVLE
                     FLKKEQIKATFFCVGENVQKHPDLYKQLIADGHQVGNHTMNHLNGMKCANEVYLNSIE
                     QATAYINSKLFRPPYGRLPKKLDKIIAQRYKIIMWSWLSKDYDNTVATQKIIAAAQQI
                     KPGDVLVFHDNSKTKERLKEVLPPIVQLLKTKDFQFQIIA"
     gene            complement(7475..>7839)
                     /locus_tag="PKN22_03025"
     CDS             complement(7475..>7839)
                     /locus_tag="PKN22_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41707.1"
                     /translation="NQPHLFSALDAYLQIFAASNAVSGNPTGELAKQTKKYVDDLLQS
                     KFKTLYSKLEASIADASESVKASKAVDLMSLKDYIEALMDSYSLGGGTEAATVNPSNV
                     DFNQLIEEQNKKDSVVQP"
BASE COUNT         2558 a         1345 c         1502 g         2434 t
ORIGIN      
        1 aaaacggatc aattaatggt gtttttttat cccattttgc caaacgaatc ataccggcag
       61 ccaacacctc gttgagtgga gcatcacccg tatcgtcgcg ataaccacgt tgatacaaag
      121 gcaagccact agagttgagt gaaataataa ctttatcttc gttgatatgc acatcaatat
      181 gcacttgcgg attttttgca tttacatccg ggcgtttgcc tgttttatca cgaaaaacat
      241 caacaatagc atcttttagc aacaaaaacg gaaatttagt atgacgaaac aagtgcgaca
      301 tcaccacacc ttttacggca aacgttttat tttcatcaaa atatgctgtc caatctattt
      361 tttttgcttg tttgtacaaa tcttgctcat cgcgaattct aaaaaacgca atttccacca
      421 aaatagaaag ggcgcaacgc aagttaaggt tcatgttgta cacatctttc cacgatgctt
      481 ccaattgaac cgcacgattc agtacaacaa tattgctgta acctaactca ataagttcct
      541 cttttaaaac gttttctaaa ccaaacaagg ttttgatggt tactaaaaga ttttcattag
      601 gatttgaagg gcgactatac ttgctcacgt ttttttcaat taaatttata taaatttgta
      661 caaaagaaag aaattgaaag caatattccg ccatcttttt gcaattgttg tgtttaccca
      721 gtcatttttt atgttggggc aagaaaaaat tggattggtt ttaagtggtg gtggtgctac
      781 tggtttggcg catattggtg taatcaaagc attggaagaa aatggaattc ctattgatta
      841 tatttcagga acatccatgg gggcattggt gggtggaatg tatgctgcag gatatagtcc
      901 cgaagaaatt gaagcttttg ccaaatcgga tgcttttttg aaaatggcta cggggcaaat
      961 tgaaacacac caacgatttg cctatcgtga gtcggatgag aatgccgcta tgtttaagtt
     1021 tggaattgcc attgatagtt ccattcttac gtctattccc actcattacc gttcatcggc
     1081 gtatgtggat tataagttgt tggaattata cggtcccgtt ggagaaatgg tccacaataa
     1141 ttttgacagt ttgtttgttc cctatcgttg tgttgcttcg gatattgcca acaaaaaatc
     1201 ggtggtgttt cgttctggaa aactcaacca agccatacgc gcgtccatga cctatccatt
     1261 ttattttgaa gcaattgaag tcaataaaac cttattattt gatgggggac tttacaacaa
     1321 ttttccagca gatgtaatgt ttaatgattt taacgttgac tatattattg gaagcaatgt
     1381 ttcttacaac aatccaaaac ccaaacgcaa taatatcatt agtcagattt ccaatatgtt
     1441 gatgttttat tccaactata ccattccgtg tgaaagtgga attttgattc aacctgatat
     1501 taaatcaact acatttgaat ttaaagatgc tgcagaaatt atagcagaag gttatcgaca
     1561 agcccaaccc tatattgatt ccattttgca acatacaacc agaagagtaa gtaaagaaga
     1621 attgcaacaa cgtagagctg cctttcgaaa taaaattatt cctgttcaaa tttccagtgt
     1681 caccaccaat atgcgcaagc aaaagccatt tagttttgca aaaaactcga tgatacgccg
     1741 aaaggaagag aaggtaaatg caaaagtatt tgagcgccgt tattttagat tgcgggcaac
     1801 acctcaaatt ggtttcttgt ttccaacatt aagtttaaaa caagacagca cttataattt
     1861 agatttaacc gtaaataaag ccaaagactt taattttgaa ataggaggac atttttctac
     1921 acgtgctgta aacacaggat ttattggtgt ttcctacagt catattggta aggtagctgc
     1981 caaggtatat gccaattcct attttggaaa attctacacc tcagtaaaaa cagaagttgg
     2041 tatggacatt ccttctactt atcctgtcaa tatttcgggg aattttgtgt taaataaatt
     2101 ggattacttt agaagctttg ccaccttttt tgcacctgaa cgtccttcgt ttcttgtaca
     2161 aaatgaaatg tatacaggat tagaatttaa acaacctatt tggaattcta ttaaaagtac
     2221 gtttggtgga cgttattttt tattagaaga ccgctattac caggataaaa acttcacaaa
     2281 tgctgacact accgatttta ctcaatttca aggttttact gctagttggg agttggttca
     2341 aaacacattg aatagaaaac aatttgcaac ttctggtcat tttgctgctt taaaaattcg
     2401 ctacatcaat ggtagagagc attcaaaatc gggtacaact gcgcccattc catttgacaa
     2461 ttataaatac cactcatgga ttagtatttc aggagaattt caatcctatc ctgttagctt
     2521 aaagtttttt catcttgggt tgcacggaaa tgctgttttc aactcacaat ccttgttttc
     2581 caactataca gcctctttac tatcactgaa tgcttataca ccattgcccg atatggctac
     2641 gtattttatg tcagagttta gaagcccaca gtatgtaggt gcaggtttaa atttagtgtt
     2701 tacaattaaa aaagtattag atattcgtgc tgatggctat tattatcaac ccttccgagc
     2761 tttggtaaaa aacgacgatg gaacttttgg atatgccaaa ccatttaaag gagaatcctt
     2821 tgttgcctct gcatctatta tctatcattc gccggttgga cccttacgcg ccacttttaa
     2881 ttatttccca aaacaacata ttccatacgc ttttcaaata agctttggtt atgttttatt
     2941 caatgcgcgt gcaattcgct aaatcaaacc ataatttttc taaaaatatt atgctcgcat
     3001 aaaatatttt gttactttta gcattgttaa atgtaactta atgtcagaca tcaatcaatt
     3061 aatcatagat tcttccattc gttcggcttg gttcaagctg tcaagaatgt ataaccaaaa
     3121 agcgttggaa cacggtctca caatgtccat tggatttatt ttaatgaaca tcgataaaga
     3181 aggtacgccc agcacgcaat tggggccgcg tatgggaatg caagccacat cactttctcg
     3241 caccttaaaa acgatggaag aaaaagggtt aattgaacgc aaagaagagg gtaatgataa
     3301 acgtaaagtt ttaattttcc taactaaaag tggtgttgac actcgccgag aagtgcgcaa
     3361 tttcttagtt gattttaata acaaagtagt agaaaatatt tcctcatcga aattaaaagt
     3421 gttttttgaa gtgattcaaa aaatagacga tttaattgac agtgaaattc aagaaaataa
     3481 aaataaataa cattaaaaat gagcagaata attaaaaaag tagccgtttt aggttctgga
     3541 gtaatgggct ctagaattgc ttgccatttt gcaaatattg gttgtgaagt tgttttattg
     3601 gacatcgttc caagagaatt aaacgatgca gaaaaagcaa aaggtttaac tttagaagat
     3661 aaagttgtac gcaacagaat tgtaaacgat gaattgcaag caacgttaaa gtcaaatcca
     3721 tcgccaattt atttgcaatc gtttgcacaa cgcattaaaa caggaaattt tgatgacgat
     3781 ttaaaagaaa ttgcatcgtg tgattgggtg attgaagttg tggtagaacg tttagacatc
     3841 aaaaaacaag tgtttgaaaa tgttgagaaa ttccgtaaac ctggaactat tatttcatcc
     3901 aacacctctg gtattccaat tcatttaatg ttggacggaa gaagtgatga ttttaaagca
     3961 catttcttag gaacgcattt cttcaaccct ccacgctatt tacaattatt ggaaatcata
     4021 ccgactcctg aaacaaaaac agaaattgtt gacttcttaa tggattttgg aagtcgctat
     4081 ttaggtaaaa agacggtttt atgtaaagac acaccagcat ttattgctaa ccgcgttggg
     4141 gtttactcca ttatggcatt gtttcatgta attaaagaag caggcttaac ggttgaacaa
     4201 gtagataaat taacaggtcc agtgatgggg cgaccaaaat cggcaacatt tagaacttgc
     4261 gacgtggttg gtttggatac cttggtgcat gttgccaatg ggttaaaagc caattgcccg
     4321 aacgatgaag agaaagcatt atttgaattg cccgactata tcactaaaat ggtagaaaac
     4381 ggttggattg gtgcaaaagc tggtcaaggg ttctacaaaa aagtgaaaaa agaagacggc
     4441 agcagtgaaa ttatggtgct agacctgaat acgttggaat accgctcgca agaaaaagta
     4501 aaatttgcta cgcttgaaat ggtgaaacct atcgaagatt tgaaacaacg taccaaaatg
     4561 ttgtttatgg ggcaagatgc ggctggtaaa ttgttcaatc aactatttgg aggtttgttt
     4621 gcttatgtaa gcaatcgcat accagaaatt gcagatgact tgtataaaat tgacgatgca
     4681 attaaagctg gttttggttg ggaacttggg ccgtttgaaa catgggactt gattggtatt
     4741 gagcaaggtt tgaaattaat tgctaaaaac aacaaaaaag cggcactttg ggtagaagac
     4801 atgaaagctg ctggctttaa ttcattctac aaatcagaag gtggtaaacg ttatttttac
     4861 gacatccaaa caaaaactta tcaaatcatt cctggtacag aaaacttggt tgatttagaa
     4921 gcattgcgca gtgagaaaaa agtttggggc aattcagacg taacggttgt tgatttaggc
     4981 gatggtattt tgaacctcga attccacaca aaaatgaaca ccattggagg tggagtgatt
     5041 gagggaatca acaaagcaat tgatttagcc gagagcaact acaaagggtt ggtgatttca
     5101 aacaacggtc aaaatttctc tgctggagct aatgtgggat tgattttcat gatggctgcc
     5161 gaacaagatt ttgatgaatt gaacttctcc attaaagcat tccaagacac catgatgcgc
     5221 gtgcgttatt ctaacattcc agtaattgtt gcacctcaca acatggcttt aggtggcgga
     5281 tgcgagttgt caatgcattc tgacaaggtg gtggcgcatg ctgaattgta catgggctta
     5341 gtagagtttg gtgttggact aattcctggt ggaggtggtt ccaaagaatt cgcaaaacgc
     5401 ttttcagacg atatgaaaaa aggcgatatt aaaatcaatc gtttcagaga tcgtttcttg
     5461 actattggac aagccaaagt ttcaacgtct gcatacgaag catttgaatt agggtattta
     5521 agacctggaa cagatgaagt tgtggttagc cgtagtcacc aattaactcg tgcaaaacaa
     5581 gccgcattgg aaatggcaaa caaaggttac attgcaccaa aacgtgagaa aaacatcacc
     5641 gttgtgggtc aagaaggatt gggaattgtg tatgtaggtg ccaactcaat gacatcagga
     5701 aaatacattt cagaacacga tcaattgatt tctgagaaat taggttttgt attgtgtgga
     5761 ggtgatttat ctgaaaacac cgtagtttca gaacaatatc tattggattt agaacgtaaa
     5821 gcctttttag agttgtgtgc tgaacctaaa acattggaac gacttcagag tttagtgaaa
     5881 tttggaaaaa tccttcgtaa ctaaaccaaa gttttcaatt ataaatctca agaaaaggta
     5941 gcgggcaata aagttcgtta ccttttcgtt ttataggata ctatcaaaat tacaacttac
     6001 aggttgtgat ttttagttat atttgaactc aaatcagcta agaattatga aatcaattat
     6061 tgtttcttca cttttaatcg ttgcattagt tgcttgcaaa agcacaacct ctaaagaagt
     6121 agagcaatct gccgctacta ccagtaatga cggtccaaca attgcagcca attcacaaga
     6181 tgataaaatg gcagctattg accagcaatt ggataaattg tctcgcattg agagtttaaa
     6241 ttattcaaaa gaagatggaa cttctatcag cgcaattgca tatatggcaa gccaagacac
     6301 cattgcaaaa gttgaagtaa aacaatacaa cgccaaaaca gttaccaatc ccaaaattca
     6361 tttttattat gacaaaggat tgttgtttgc tagcaaaaaa ttggaagaaa aaataattga
     6421 tcaaacggct tatttttctg aagaagtatc gttttataac gaaaaaggag atgtaattca
     6481 atccaaagaa cgcatatcgg actatgaaga agggttgatt aacgcggcgt ataaaaaaat
     6541 caacaatgtt actcacgacc caacagaagc atttttggta ttgaagcaac agggtccttt
     6601 tgccgttact tttcaaggtt ttgttgacaa tggaccttat catttcttaa ttgttggtga
     6661 aaacgttgca aaagatggtt atacagctgc tctttccatt caaaaagaag atgccaccat
     6721 taaataccta agaaacgaag gaaaaaaagc gttgggcaaa gaattaatgg tgcaatttgt
     6781 aaaccacatg gacaatcaag gttatgaaat gcaaattcta actgatttgg cattagtgga
     6841 gcgtaaaaag aaataaatct tacgctataa tctgaaattg aaagtctttt gtttttaata
     6901 gttgaacaat tggaggcaac acctctttca atcgttcttt agttttacta ttatcgtgaa
     6961 acaccaacac gtccccaggc ttaatttgtt gggctgctgc aatgattttt tgagttgcaa
     7021 ccgtattatc gtaatcttta gataaccacg accacattat aattttatag cgttgggcaa
     7081 tgattttatc caacttttta ggtaaacgtc cataaggcgg acgaaacaac tttgaattaa
     7141 tgtaagctgt tgcttgctca atggagttta aatacacttc attagcgcat ttcataccgt
     7201 ttaaatgatt catcgtgtga tttccaactt gatgtccatc tgcaattaat tgtttgtata
     7261 aatcaggatg tttttggaca ttctcaccca cgcaaaaaaa cgtggcttta atttgttcct
     7321 tttttaagaa ttcaagcacc caaggagtaa tgtcaggatt ggggccgtcg tcaaaagtta
     7381 aaaaaacgat gggttcagaa acagcaatcc cccaaatcct taaaggaaat agccttctaa
     7441 gaattttggg ggatcgaaac atgtttatgt atttttaagg ttgaacaaca gaatcttttt
     7501 tattctgctc ttcaatcaat tgattaaaat caacgttgga tgggtttaca gtggctgctt
     7561 ctgtgcctcc acctaaacta tagctatcca ttaatgcttc aatgtagtct tttaatgaca
     7621 tcaaatcaac tgctttactt gctttaacac tttcactagc atcggcaata gaagcttcta
     7681 atttgctata caacgttttg aatttagatt gcaacaaatc atccacgtat tttttagttt
     7741 gttttgccaa ttcgcctgtt gggtttcctg aaacagcgtt tgaagcagca aatatttgca
     7801 aatacgcatc caatgcacta aacaaatgtg gttggttgt
//
LOCUS       DAOQVQ010000058         7766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10356_length_7766_cov_4.071456, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000058 DAOQVQ010000000
VERSION     DAOQVQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10356_length_7766_cov_4.071456"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /gene="thrS"
                     /locus_tag="PKN22_03030"
     CDS             <1..1219
                     /gene="thrS"
                     /locus_tag="PKN22_03030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNS41708.1"
                     /translation="HRKLGKELELFTFSQTVGQGLPLWLPKGAALRERLENFLKRAQK
                     QSGYLQVITPHIGNKDLYVTSGHYAKYGADSFQPIRTPDVNEEFLLKPMNCPHHCEIF
                     KSRPRSYKELPTRFAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTQDQVKDEFKK
                     VIDLVLYIFKTLSFTNYKAQISLRDPENPSKYIGSDENWEKAERAIIEAASEKGLPTV
                     VEYGEAAFYGPKLDFMVQDALGREWQLGTIQVDYNLPERFELEYIGADNQKHRPVMLH
                     RAPFGSMERFVAVLLEHCAGDFPLWLTGSQFIVLPISEKYNEYAEKVSQYLNNSDIRG
                     SVDDRNEKIGKKIREAELNKIPFMLIVGEQEEQNNQVSVRQRFEGDLGAMSADEFKNL
                     IEKLVEKELVFNV"
     gene            1229..1780
                     /gene="infC"
                     /locus_tag="PKN22_03035"
     CDS             1229..1780
                     /gene="infC"
                     /locus_tag="PKN22_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNS41709.1"
                     /translation="MSKLKGGKPVRKDQQQDEHRINERITAPQIRLVMEGEEPKVMTV
                     REALVLAEEAELDLVEISPNAVPPVCKLMDYRKFLYNQKKKQKELKAKQTKITIKEIR
                     FGPNTDDHDFNFKLGHAKKFLEEGSKVKAYVFFRGRTIVFKDRGEILLLKFAQELAEV
                     GALEQMPKLEGKRMTIMLNPKKK"
     gene            1865..2062
                     /gene="rpmI"
                     /locus_tag="PKN22_03040"
     CDS             1865..2062
                     /gene="rpmI"
                     /locus_tag="PKN22_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNS41710.1"
                     /translation="MPKMKTKSSAKKRFSITGSGKIKRKHAFKSHILTKKSTKRKRNL
                     THAGLVHTADESNIKLQLGLK"
     gene            2154..2501
                     /gene="rplT"
                     /locus_tag="PKN22_03045"
     CDS             2154..2501
                     /gene="rplT"
                     /locus_tag="PKN22_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNS41711.1"
                     /translation="MPRSVNHVASRARRKNVLKLAKGYFGRRKNVWTVAKNAVEKGLS
                     YAYEGRKQKKRNFRSLWIARINAGARMNGMSYSQFMGAVHKSGIELNRKVLADLAMNN
                     PEAFKAVVNAVKK"
     gene            complement(2555..3733)
                     /gene="mnmA"
                     /locus_tag="PKN22_03050"
     CDS             complement(2555..3733)
                     /gene="mnmA"
                     /locus_tag="PKN22_03050"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNS41712.1"
                     /translation="MKRVVVGLSGGVDSSVTAHLLIQQGYEVIGMFMRNWHDESVTLE
                     DECPWIDDSNDALLVAQHLGIPFQVIDLSKEYKERIVDYMFSEYEAGRTPNPDVLCNR
                     EIKFDVFLKAALDLGADYVATGHYCRKIQHDDGSFGLLAGKDNNKDQSYFLCQLTQEQ
                     LSKALFPIGELQKSEVRAIATEIGLVTADKKDSQGLCFVGKISLPVFLQQQLAQKQGN
                     VIEIPADFPPFSAYHQLAHTEENLVALTTPFTFQESDGLKVCEHIGAHYYTIGQRKGL
                     HIGGRPHPSFVIGIDTKTNTVYTGQEETHPGLNRAALFINNDEISYINKKYALQDGES
                     LNCKVRIRYRQPLQAATLYKKPEGMYILFEELQKGITPGQFAAFYLGEELIGSGTIQQ
                     "
     gene            3878..5356
                     /gene="ric"
                     /locus_tag="PKN22_03055"
     CDS             3878..5356
                     /gene="ric"
                     /locus_tag="PKN22_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNS41713.1"
                     /translation="MQELNEAIIDVQSIEPRLRHQTIFQEFHNLHNGESFIIHNNHDP
                     KPVYYQLIDIFGEVFTWDYLQAGPEWWDIRVTKTEDTNEGEEKDLVLNVPAIEPQFKH
                     ETIFNTFKSLRPGNSFIIHNDHDPKPVYYQLINLFGEAFTWEYLQQGPQWYDIRVTKN
                     GEAPAKVEEKPVVATNVTVGNDNEKVVDVPSLEPRLKHPTIFQTFDTLQAGESMVIHN
                     DHDPKPVYYQLLSERGDVFTWEYLQQGPQWWDIRVTRKGTETTETIGEIVAKDLRKAE
                     VFKKFGIDFCCGGKKTVRQVCAEKGIDPTIVEQELQKPVTGVTSTDMNYTEWDLDFLA
                     DYIVNKHHKYVRKYLPEIVKYATKVAQVHGGGHPELHEIKRLFDQVDAEMSQHMIDEE
                     QKLFPLIKEIVRAKEGYSTYQKNVSFADMVAETEKEHDSVGRALEKIRELSANYTIPE
                     DACTSYKLLFAMLQEFENDLFTHIHLENNILFVKAAEVEKSL"
     gene            5461..6276
                     /locus_tag="PKN22_03060"
     CDS             5461..6276
                     /locus_tag="PKN22_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNS41714.1"
                     /translation="MIINERTKIAQLLKHHPDALETIVSIAPDFKKLRNPILRKLMAG
                     RTTIAMASKIGGCKPEDFFKALAPLGFEADSKKTVSEAAPENKPLPDYLKTLPSQQLV
                     NFDVRAMLASGEDPLKSIQQKVNALKEGQALLIINNFEPVPLIKLLERQGFQAYVRFV
                     DAETIETYFYKENATSAPLQAEEVKDSDDWDSVLKYYEGKLEEVDVRHLEMPMPMMTI
                     LGALDTLPEDKALFVNHKKIPVFLLTELKDRGFEYRIKDVQEGEVYLLIFKEK"
     gene            6279..7559
                     /locus_tag="PKN22_03065"
     CDS             6279..7559
                     /locus_tag="PKN22_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41715.1"
                     /translation="MSGPTFSGNLQQTTNYKVVLPFYVYGAISFVIACTLLLFNTDLV
                     QLHYFTPHTLAIVHAMALGWATMIILGASHQLLPVLIEGKLDSDTIAFTTFYITAVGI
                     PILIYGFYIFHSGWVMQLGAILINIGILFYVINVLRSSIKSSTHGVHAWYMITASLWL
                     FTTTFFGLLLVFNFNYQLLPSSSVSYLSIHAHLGIIGWFLLLVIGVGSRLIPMFFISK
                     YTNEKVLWIIYALINCSLIAFVIIKLIALPTYFYYLPLILAFTGIFLFVRHIIKAHAV
                     RIRRKVDEQMKTSILSVFQLMVPVVVLLLTLIFLPMEQHTNMALIYGFSIFFGWLTAI
                     ILGMTFKTLPFIVWNREYHKRARQGKTPAPKELFNEKVYNTMLVCYLLGFVSFIVALS
                     IKNDFVAFGATLALLLAAILYVFNVGLTVFHKAN"
     gene            7561..>7766
                     /locus_tag="PKN22_03070"
     CDS             7561..>7766
                     /locus_tag="PKN22_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNS41716.1"
                     /translation="MSSVCVMTNDSEKCDFALVGLYDVYDPEVGLNVVDLGLIYQIDF
                     IEDQMRVHCLMTLTTQFCPMGDQIT"
BASE COUNT         2572 a         1331 c         1543 g         2320 t
ORIGIN      
        1 ccaccgtaaa ttggggaaag aattagaatt atttactttc tctcaaactg ttggtcaagg
       61 tttgcctttg tggttaccta aaggtgctgc attgcgtgag cgtttggaaa acttcttgaa
      121 acgtgcacaa aaacaatcgg gttatttaca agttattacg ccgcacattg gaaataaaga
      181 tttgtatgta acttctggcc actatgcaaa atatggtgcc gattcgtttc aaccgattag
      241 aacaccagat gttaacgagg agtttttgct aaaaccaatg aactgtccgc accactgcga
      301 gatttttaaa tcaagaccac gttcatacaa agaattgcct actcgttttg ctgaatttgg
      361 aacggtatat cgctacgagc aatcgggtga gttgcacggg ttgacacgtg tacgtgggtt
      421 tacacaagac gatgctcata ttttctgtac gcaagaccaa gtaaaagacg agttcaaaaa
      481 agtaattgac ttggtattgt acattttcaa gacattatcg tttactaatt acaaggcaca
      541 aatttcattg cgcgacccag aaaatccttc taaatacatt ggaagtgatg agaattggga
      601 gaaagcagaa cgtgccatca ttgaggcagc atctgaaaaa ggattgccaa ccgtggttga
      661 atatggtgaa gcagctttct atggtccaaa attggatttc atggtgcaag atgcattggg
      721 tagagaatgg caacttggaa cgattcaagt ggactacaat ttaccggagc gttttgagtt
      781 ggaatacatt ggggcagaca atcaaaaaca ccgtccagta atgttgcacc gcgcaccgtt
      841 tggttcgatg gaacgttttg tggctgtgtt acttgagcac tgtgctggag atttcccatt
      901 gtggttgaca ggaagccaat ttatcgtact tccaatatca gaaaaataca acgagtatgc
      961 agaaaaagtt tcacaatatc taaataattc cgatattcgc ggttccgtag acgaccgaaa
     1021 tgagaaaatc gggaagaaaa tacgtgaggc agagctcaat aaaatcccat ttatgttaat
     1081 agttggagag caagaggaac aaaacaatca agtttctgtt cgtcagcgat ttgaaggaga
     1141 tttgggagca atgagtgcag atgaatttaa aaatttaata gaaaaactgg tagaaaaaga
     1201 acttgtattt aacgtatagt ttaattgaat gagtaaatta aaaggaggaa aacctgttcg
     1261 taaagatcaa caacaagacg aacacagaat taacgaaaga ataacggcgc cacaaatccg
     1321 tttggtaatg gagggcgaag aaccgaaggt aatgaccgtt agagaagctt tggtattagc
     1381 agaagaagca gaattagatt tagtagaaat ttcgcctaat gctgttccgc ctgtttgtaa
     1441 actcatggac tatcgtaagt tcttatacaa ccaaaagaaa aaacaaaaag agttaaaagc
     1501 aaaacaaacg aaaattacaa ttaaggaaat tcgttttggt ccgaacacag acgatcatga
     1561 ttttaacttt aaattaggac acgctaaaaa attcttggaa gaaggaagta aggtaaaagc
     1621 ttatgtgttc tttagaggac gtacgattgt gttcaaagat agaggggaaa tattattgtt
     1681 gaagtttgca caagaattgg cagaagtagg tgctttagag caaatgccaa aattggaagg
     1741 taaacgtatg acaattatgt tgaacccaaa gaaaaagtaa atagacattc acagattagt
     1801 tgtcgtaaaa cacaaataac tgtaaaagaa gctatataga ggtaacttta aaaattgaag
     1861 agagatgcca aaaatgaaga ctaaatctag tgcaaagaaa agattttcta tcactggaag
     1921 cggtaaaatt aaaagaaagc acgcattcaa aagtcacatc ttgactaaga agtcgacaaa
     1981 aagaaagcgt aatttgacac acgcagggtt agtacacact gctgatgagt caaacattaa
     2041 attacaattg ggattgaaat aagcatccca ggtttattaa ttgtttaacc cggtaatgag
     2101 taactaagtc gtttgcaaca atagcgcact ccttatcaaa aaaattaaag attatgccaa
     2161 gatcagtaaa tcatgttgct tcaagagcac gtcgtaagaa tgtgttgaag cttgccaaag
     2221 gttactttgg acgtagaaaa aacgtttgga cagttgcaaa aaacgccgtt gaaaaagggt
     2281 tgagttatgc atacgaagga cgtaaacaaa agaaaagaaa cttccgttca ttgtggattg
     2341 ctcgtatcaa cgcgggtgct cgtatgaatg gaatgagcta ttctcaattc atgggtgcag
     2401 ttcacaaaag tggaatcgaa ttgaaccgta aagttcttgc agatttagcg atgaacaacc
     2461 cagaagcttt caaagctgtt gttaacgcag ttaagaaata agtaataatt ttctctatat
     2521 agtaaaaatc cttcccattt tgggagggat ttttttattg ttggatagtt cccgaaccaa
     2581 tcaattcttc acccaaataa aatgcggcaa attgccccgg tgtgatgcct ttttgcaatt
     2641 cctcaaacaa aatatacatt ccctctggtt ttttatacaa cgtggctgct tgcaacggct
     2701 gacgataacg aatgcgtacc ttgcaattca acgattcacc gtcttgtaat gcatattttt
     2761 tgttgatata actaatttca tcgttgttga tgaacaaagc tgcacggttc aaccctggat
     2821 gggtttcctc ttgccccgta taaacggtgt tggttttggt atcaatgcca atcacaaaag
     2881 atggatgcgg gcgcccacca atgtgcaatc ctttgcgctg cccaatggtg tagtaatgtg
     2941 ctccaatgtg ttcacacact tttaaaccat cactttcttg gaaagtaaaa ggcgttgtga
     3001 gtgccaccaa gttttcttct gtatgtgcta attgatggta ggctgaaaat ggtgggaaat
     3061 cggctggaat ttctataaca ttaccttgtt tttgtgccaa ttgttgctgt aaaaacaccg
     3121 gtaaactgat tttacccaca aaacataagc cttgcgagtc ttttttatcg gctgttacca
     3181 atccaatttc tgttgcaatg gcacgcactt ccgatttttg caattcacca attggaaaca
     3241 atgctttgga taattgttct tgggtgagct gacacaaaaa ataagattga tctttattgt
     3301 tatcctttcc tgccaacaag ccaaaacttc catcatcgtg ctgaatttta cggcaataat
     3361 gtccagtagc aacataatct gcacccaaat ctaaggctgc ttttagaaaa acatcaaatt
     3421 ttatttcacg gttgcacaac acatccggat ttggtgttct acctgcttcg tattcgctaa
     3481 acatgtagtc aacaatgcgt tctttgtatt ctttacttag gtcgataacc tgaaacggaa
     3541 tacctaaatg ctgtgccact aacaaagcat cgttggaatc atcaatccac ggacattcat
     3601 cttctaaggt cacggattcg tcgtgccaat tgcgcataaa cattccaatc acttcatatc
     3661 cttgctgaat caataaatgt gcagtaacac ttgaatcaac accaccagaa agtccaacaa
     3721 ctactctttt catgttgcaa aattactgat tttttaaatc ttactgctaa aaccaattga
     3781 ttttgatgga caaaaaaata ttttatgtat aaaagtactt taatactttt attattaaat
     3841 tttacttata tttgcacaag attttaaatt tagatttatg caagagttaa atgaagcaat
     3901 tattgatgtg caatcaatag agccaagatt aagacatcag acgatatttc aagaatttca
     3961 caatttacac aatggtgaaa gctttattat ccacaacaac cacgatccaa agccggttta
     4021 ttatcaatta attgacattt ttggtgaagt ttttacgtgg gattatctac aagctggacc
     4081 agaatggtgg gacattcgtg ttactaaaac agaagacacc aatgagggtg aagagaaaga
     4141 tttggtgttg aacgttcctg ctattgaacc tcaatttaaa cacgaaacaa ttttcaatac
     4201 gtttaaaagt ttacgtccag gaaattcatt tatcattcat aatgatcacg atccaaaacc
     4261 tgtatattac caattaatca atttatttgg tgaagcattt acgtgggaat atttacaaca
     4321 aggtccacaa tggtatgaca ttcgtgttac aaaaaatggt gaagctccag caaaagttga
     4381 agaaaaacct gtagttgcaa ctaacgtaac agtgggtaac gataacgaaa aagtggtgga
     4441 cgttccatct ttagaacctc gtttaaaaca cccaactatt ttccaaacat ttgatacatt
     4501 gcaagcaggc gaaagcatgg taattcacaa cgaccatgat ccaaaacctg tttactatca
     4561 attgttgagc gaaagaggag atgtatttac gtgggaatat ttgcaacaag gtccacaatg
     4621 gtgggatatt cgcgtgacac gtaaaggaac tgaaacaaca gaaactattg gtgaaattgt
     4681 agctaaagat ttgcgcaaag cagaagtttt caaaaaattt ggaatcgatt tttgttgcgg
     4741 tggaaagaaa acagtgcgcc aagtttgtgc tgaaaaaggt atagacccaa caattgtgga
     4801 gcaagaattg caaaaacctg taacaggtgt aacatcaaca gatatgaact acactgagtg
     4861 ggatttggat ttcttggctg attatattgt caacaaacac cacaaatacg ttagaaaata
     4921 cttgccagaa atagtaaaat atgcaacaaa agtggcacaa gtgcatggtg gtggacatcc
     4981 agaattgcac gaaattaaac gcttgtttga ccaagtagat gcagaaatga gtcagcacat
     5041 gatagatgaa gagcaaaaat tgtttccact tatcaaagaa atcgttcgtg caaaagaagg
     5101 atattctact taccaaaaaa atgtaagctt tgctgatatg gttgctgaaa ccgaaaaaga
     5161 gcacgacagt gtgggtagag cactggaaaa aatccgtgaa ttgagcgcaa attacactat
     5221 tccagaagat gcttgtacaa gttacaaatt gctttttgca atgttacaag aatttgaaaa
     5281 tgatttgttt acacatatcc atttagaaaa caacatcttg tttgtaaaag cagcagaagt
     5341 agaaaaaagt ttataaaacc ataaaaaaac aacttattcg gtcggagtgc tgaataagtt
     5401 gttttttgct ttttatttag aattattctt aataaatttg cttatcaaac ttttgagcaa
     5461 atgataatca acgaaagaac caaaatagcg caattattga aacaccatcc cgatgcgttg
     5521 gagaccattg tttcgattgc acccgatttt aaaaaattac gtaatccgat attgcgtaaa
     5581 ttaatggctg gaagaacaac cattgccatg gcctcaaaaa ttggtggatg caaaccagaa
     5641 gattttttta aagctttagc accacttggt tttgaagcag attcaaaaaa aacagtttca
     5701 gaagctgctc ctgaaaacaa acccctaccc gattatttaa aaacacttcc atcgcagcaa
     5761 ttggttaatt ttgatgtgcg tgccatgttg gctagtggcg aagatccatt aaaaagcatt
     5821 cagcaaaagg taaatgcatt aaaagaaggg caagccttgc ttatcatcaa taattttgag
     5881 cctgttccgc tgattaaact attagaacgc caaggttttc aagcttatgt acgttttgtg
     5941 gatgctgaaa caatagaaac ttatttttac aaagaaaatg ctacctctgc tccattgcaa
     6001 gcagaagaag taaaagattc tgacgattgg gacagtgttt tgaaatacta cgaaggtaag
     6061 ttagaagaag tggatgttcg tcatctagaa atgccgatgc caatgatgac tattttggga
     6121 gctttggaca ccttgcctga ggataaagca ttgtttgtga accataaaaa aattcctgta
     6181 ttcttgctca ccgaactaaa agacagaggt tttgaatacc gcatcaagga tgtgcaagaa
     6241 ggcgaagtat atttattgat ttttaaagag aaataagcat gtcgggacca acatttagtg
     6301 gaaatctaca acagacaacc aactacaaag tcgtgttgcc tttttatgtc tatggcgcaa
     6361 tttcttttgt gattgcttgt acgttgctac ttttcaacac ggatttggtt caacttcact
     6421 atttcacgcc tcacacgttg gccattgttc atgcgatggc attgggatgg gcaaccatga
     6481 taattttagg agcatcacac caattacttc ccgtattgat tgaaggaaaa ttggacagtg
     6541 acaccatcgc attcactact ttttacataa ctgccgttgg aataccgata ttaatttacg
     6601 ggttttatat ctttcacagt gggtgggtca tgcaattggg cgccattctc attaatatcg
     6661 gtattttatt ttatgttatc aatgttttaa gaagttcaat taaaagttca actcacggag
     6721 tgcatgcttg gtacatgatt acggcttctt tgtggttgtt taccactact ttctttggat
     6781 tattattggt atttaatttc aattaccaac tattgcctag tagttcggtt agttaccttt
     6841 ccatacatgc gcatttggga attataggtt ggtttttgtt gttagttatt ggggttggct
     6901 cccgtttgat tcctatgttc tttatttcca aatacaccaa cgaaaaagta ttgtggatta
     6961 tttacgcatt gataaactgt agtttaatag cctttgtaat catcaagcta atagcattgc
     7021 ccacttattt ttattacctt ccgttgatat tggcatttac aggcatcttt ttgtttgttc
     7081 gtcacatcat caaagcgcat gcagtgagaa taagaagaaa agtggacgag caaatgaaga
     7141 cttccatcct atcggttttt caattgatgg ttcctgttgt agtgttattg cttactttga
     7201 tatttttacc aatggagcaa cataccaaca tggcattaat ctatggattt agcattttct
     7261 ttggatggct aacggcaatt attttaggaa tgacattcaa aacgttgcct tttattgtgt
     7321 ggaacagaga ataccacaaa cgtgcgcgac aaggaaaaac acctgctccc aaagaattat
     7381 ttaatgaaaa ggtgtacaat accatgcttg tttgttactt gcttggattt gtatcgttca
     7441 ttgtggcatt aagtattaaa aatgatttcg ttgcctttgg tgcaacacta gctttgctct
     7501 tagcagcaat tttatatgta tttaacgtag gtttaaccgt atttcacaaa gcaaattaat
     7561 atgagttcag tatgtgtaat gaccaacgac agtgaaaaat gcgattttgc attggttggt
     7621 ttgtatgatg tgtatgatcc ggaagtagga ttgaatgtag ttgatttagg attgatttat
     7681 caaattgatt ttattgaaga ccaaatgcgg gtgcattgtt taatgacttt gaccactcaa
     7741 ttttgcccaa tgggcgatca gattac
//
LOCUS       DAOQVQ010000059         7765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10357_length_7765_cov_4.768482, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000059 DAOQVQ010000000
VERSION     DAOQVQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10357_length_7765_cov_4.768482"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PKN22_03075"
     CDS             <1..1723
                     /locus_tag="PKN22_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNS41717.1"
                     /translation="VNKVVMVDATGVLKWIDRDSLGNAGGFVECSDMSGDANLSANSK
                     VNLNNNNLYFENNDALGLNHIGIGYDCSNTLSAKLSVQQVHPSIVNTSTTALSAINND
                     IGNTQGSVFTGIEGKALATQTALQIINRGSYFTAENAGHVRGVVSEIPAFSTYCFTAY
                     GGEFNVSSDARDNYGVIANSTGDGPQTENKAIVGYAKNSKIHNIGGYFNANSLGTISG
                     RSIGVDAVAEFSDQLNIGGSFKSICNITATGVSIGVSGIAFEGGYSSNTYPGSTFIGV
                     YGAALPTSLGNPSLYAGYFDGDVYINGPTSGTGYALTSDILFKTDTATISSSVADSLL
                     LLLKPKSYYMDTLNNYGMNFSSKKQFGLVAQDVENVIPNIVNENHKPAMIDTMGNVII
                     QGVDYKTLNYTELIPLLIAGHQEQSSKIDSLQTANDSLQTQITDLNNRLSQLENCLSG
                     ILPFLCQLSQNAIEENGKVAQEELRHIIEVELHNGASIVLNQNVPNPFAEKTVITYTI
                     PQSVQEAQIVFYDATGSLIKTIDIRERGNGQINVYAQDLSSGTYTYSLVADGKVVATK
                     RMVKAGF"
     gene            complement(1804..3588)
                     /locus_tag="PKN22_03080"
     CDS             complement(1804..3588)
                     /locus_tag="PKN22_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS41718.1"
                     /translation="MSEKNTIKGGEFLIKDTLPHEIFTPEEWTEEQRMIAQMCDDFIT
                     QEVTPNLDRIDAMEDGLMVSLLEKAGELGLLGLTVPEDLGGMGQDFKTSLLATEYLGK
                     GYSFSVAYGAHTGIGTLPLLYYGNEAQKAKYIPKLASGEWKASYCLTEPSAGSDANSG
                     KTKAVLSEDGKHYILNGQKMWITNGGFAQLYTVFAKIDNDEKLTAFLVEADSEGISLN
                     AEEKKMGIKGSSTRQIFFNNVKVPVENMLSERENGFKIALNILNIGRIKLGAGVLGGA
                     KEAITESIKYANEREQFGRPISKYGAIRHKIAEQTIRTFVAESALYRAGQNIDDAIQA
                     YMESGMEKGAATLKGIELFAPECAIIKVAGSETLDYVVDEAVQIFGGMGYSAESSVER
                     AYRDSRINRIFEGTNEINRMLTVEMVLRRAMKGELDLMGPAMAVANELMSIPSFEDAP
                     EGVLGKEQAALKGFKKAILMAAGSAVQKLMTTLSKEQEVLMGIADMAITTYLAESVLL
                     RVQKMVAAKGEEACAVQIAIAKTYFYDAADKINKLCKDTLNSFAEGDELRMMLMGLKR
                     FTKLESINPKETRQLIAQAQINNGGYAL"
     gene            3732..5162
                     /locus_tag="PKN22_03085"
     CDS             3732..5162
                     /locus_tag="PKN22_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS41719.1"
                     /translation="MRYFVFIVLVFQLIGASAQMTFSTLKHQFGDLQSYDNRFVDITI
                     TNKGPKDGYILSVRKPSEVVYIQNRAFVQKDSSLTLRFQVNPQQKGKFSYTIQIFTSD
                     KAEAHQLVLSGNLVELEQNSNSYLTACPDFNTHPAGKNPNQFDLIVVTIDKNTRQELE
                     QSKVSMLQDGLALWTEKTDKSGRIKKSGSIGLAYFYATHTNYLPAEKGAFVSNERNKV
                     IIELEPIAQPEPVVEPVPEIAQQTPEPPKQEPPTGQNPPEQVVIEIEETQPPIASSES
                     IPALDSISDDNFDSNLFAPVNVVFVMDVSSSMNQANKMELMKYSINQLSEMIRQEDKI
                     ALVTYASQAKVLLPPTSGVDKEAVQEQVKLLRAGGMTAGGEGIKLGLEQARKAYIKGG
                     VNHVFVITDGAFNQKQYNYKKLLKKFTKQGIQLSVVGVLNDALARKSMKEIAQLGHGD
                     YISVAKLADAQKNIRQAVRKMAYKQK"
     gene            5217..5759
                     /locus_tag="PKN22_03090"
     CDS             5217..5759
                     /locus_tag="PKN22_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNS41720.1"
                     /translation="MSFNAWHDVSFGDEAPQVVKGIIEIPKNSRAKYELDKDSGLICL
                     DRVIASPMSYPANYGFIPQTYCDDKDPLDILVLTDVEMVPLCLVEAKIIGVMQMIDGG
                     EMDDKIIAVAKGDKNWNHINDISELNPHILDEIRVFFEDYKKLEKKSTEVKGFLNREE
                     AIRIFEKSVVDYKEKFAVKA"
     gene            5847..7136
                     /gene="tyrS"
                     /locus_tag="PKN22_03095"
     CDS             5847..7136
                     /gene="tyrS"
                     /locus_tag="PKN22_03095"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNS41721.1"
                     /translation="MKNFIQEVSWRGMLHDVMPETEEHLLEQMRTAYVGIDPTADSLH
                     VGHLVGVMLLKHFQNAGHKPLALIGGATGMIGDPSGKSNERNLLDEATLKHNQACIQK
                     QLAKFLDFESNAENAAEMVNNYDWMKNFSFLDFIRDVGKHITVNYMMAKDSVKKRLNG
                     EANDGMSFTEFTYQLVQGYDFLHLYKTKNCTLQMGGSDQWGNITTGTELIRRIGGGKA
                     FALTCPLITKADGTKFGKSEKGNIWLDEEKTTPYEFYQFWLNTSDEDAAKYIKIFTFR
                     SKEEIEALIQEHQEAPHLRVLQKALAEDLTITVHSEQALKTAIAASNILFGKSTSDDL
                     KQLSEKDFFSVFAGVPQATISKEEFKSLTIVDALAGKTGFLASNGEAKRELKANAVAV
                     NKEKVDENFTFSDEHLINGKYILIGKGKKNNYIIIVE"
     gene            7228..7449
                     /locus_tag="PKN22_03100"
     CDS             7228..7449
                     /locus_tag="PKN22_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41722.1"
                     /translation="MKKLALALTLVFATGYISNAVYAATSTNYTVFQDGNKKKSNSKK
                     SCHSTSSDTSKKSCSGEASKKSCCSAEKK"
     gene            7520..>7765
                     /locus_tag="PKN22_03105"
     CDS             7520..>7765
                     /locus_tag="PKN22_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS41723.1"
                     /translation="MEKVLEIVNLTKKYKNLTAVDNLNLSVEKGNIYGLLGPNGSGKS
                     TTLGVILNVVNATSGSWTWFNETPTTQSLQRIGAIIER"
BASE COUNT         2557 a         1442 c         1453 g         2313 t
ORIGIN      
        1 agtgaataaa gttgtgatgg tagatgcaac aggagtgttg aaatggattg atagagattc
       61 gttgggaaat gcaggtggat ttgtagaatg ttctgatatg agtggagatg ctaatctttc
      121 tgccaattca aaagtaaatt tgaacaataa caacttgtat tttgaaaaca atgatgcttt
      181 aggactaaac catattggaa taggttacga ttgttctaac actttgagcg caaaattaag
      241 tgtacagcaa gttcatccat ctattgttaa tacatcaaca acagccttat cagcaattaa
      301 taacgatatt ggcaacacac aaggatcagt ctttacagga attgaaggta aagcattagc
      361 tacacaaaca gcattacaaa taataaacag aggtagttat tttaccgctg aaaacgcagg
      421 gcatgttaga ggtgttgtgt cagaaattcc agcattctca acctattgtt ttacagcata
      481 tggaggagaa tttaatgttt catctgatgc cagagacaat tatggtgtaa tagcaaactc
      541 tactggagat ggtccacaga cagaaaacaa ggcaattgta ggatatgcta aaaattcaaa
      601 aattcataat attggaggat attttaatgc taattccctg ggtacaattt caggaagaag
      661 cataggagtt gacgctgttg ccgaattttc tgatcaatta aatattggag gaagttttaa
      721 atcaatttgt aatattactg caacaggagt gtctattggg gttagtggta ttgcatttga
      781 aggaggctat agttcaaata cgtatcctgg ttcaactttt attggagttt atggggctgc
      841 tttaccaact agcttaggga atcctagtct atacgcaggt tattttgatg gtgatgttta
      901 tattaatggg ccaacatctg gcacaggtta tgcattaaca tctgacattt tatttaaaac
      961 agatactgcc acaataagtt cctccgttgc tgatagttta ttattattat taaaacctaa
     1021 atcatattac atggatacgt taaataacta tggtatgaat tttagttcca aaaaacaatt
     1081 tggcttggtt gcacaagatg ttgaaaatgt aatacctaac attgtaaatg aaaatcataa
     1141 accagcaatg attgatacca tgggaaatgt aataatccaa ggcgttgatt acaaaacctt
     1201 aaattatact gagttaattc cacttctaat cgcaggtcac caagaacaaa gttctaaaat
     1261 tgactctttg caaactgcaa acgattctct tcaaactcaa attacagatt taaacaatcg
     1321 cctttctcag cttgaaaatt gtttgagtgg tatattgcca tttttatgtc aattgagcca
     1381 aaatgccatt gaagaaaatg gtaaagttgc acaagaggaa ttgcgccaca tcattgaagt
     1441 tgaattgcat aacggagcat caatcgtttt aaatcaaaac gtgccgaatc catttgctga
     1501 aaaaacagtg atcacttaca ccattccaca aagtgttcaa gaagcacaaa ttgtgttcta
     1561 tgacgcaaca ggttcgctta tcaaaacaat tgatattcgt gagcgtggaa acggacaaat
     1621 caatgtgtat gcacaagatt taagttctgg aacttacact tactcattag ttgccgacgg
     1681 taaagttgtt gcaacaaaac gtatggtaaa agcaggtttt tagttctact ttctcaaccc
     1741 ccactaaaaa acagggcgtt cgatacattc aaacgccctg taaatttatt tgattaaccg
     1801 tttttacaac gcatagccac cattgttgat ttgtgcttgt gcaatcaatt ggcgagtttc
     1861 tttcgggttg atgctttcta attttgtaaa gcgttttaag cccatcaaca tcatgcgcaa
     1921 ttcatcgccc tctgcaaatg agttcaaggt gtctttacac aatttattga ttttatctgc
     1981 cgcatcgtag aaatatgttt ttgcaatagc aatttgaaca gcacatgctt cttcaccttt
     2041 tgctgcaacc attttctgaa cacgcaacaa taccgactct gccaaatagg tagtgatggc
     2101 catatcagca atacccatca atacttcttg ctctttcgac aatgttgtca ttaatttttg
     2161 aacagccgaa cctgccgcca tcaaaatagc ttttttgaaa ccttttaacg ccgcttgttc
     2221 tttgcccaac acgccttctg gcgcatcttc aaagcttgga atgctcatca attcgttggc
     2281 aacagccatt gctggtccca ttaaatccaa ttcacctttc attgctctgc gtagtaccat
     2341 ttctactgtc aacatgcggt tgatttcgtt ggttccttca aaaatacgat tgatgcgtga
     2401 gtcgcggtag gcacgttcca cagatgattc tgccgaatag cccatacctc caaaaatttg
     2461 caccgcttcg tccacaacat aatccaatgt ttcagaacct gccactttga taattgcaca
     2521 ttctggagca aacaactcaa ttccttttaa ggttgcagca cctttttcca ttccagattc
     2581 catataagct tgaatggcat cgtcaatgtt ttgtcctgca cgatacaacg ccgattctgc
     2641 cacaaaagtg cgaatggttt gctctgcaat tttatgacga atggctccat atttagaaat
     2701 tggtcttcca aattgctcgc gttcgttggc atatttgata gattctgtaa tagcttcttt
     2761 agctccaccc aaaactccag cgcctaattt aatacgcccg atatttaaaa tgttcaatgc
     2821 gattttaaat ccgttttcac gctcagaaag catgttttct accggaactt ttacattgtt
     2881 aaagaaaatt tgacgtgtag aagaaccttt aatacccatt ttcttttctt cggcattcaa
     2941 tgaaattcct tcagaatcag cctctactaa aaacgctgtc aatttttcat cgttgtcaat
     3001 ttttgcaaaa acggtgtaca attgtgcaaa acctccgttg gtaatccaca ttttttgtcc
     3061 gtttaagatg tagtgttttc catcttcgct caaaacagct tttgtttttc cagagttggc
     3121 atccgatcca gcacttggtt ctgtcaaaca atacgatgct ttccattctc cagaagccaa
     3181 tttaggaatg tattttgctt tttgcgcttc gtttccgtaa tacaacaatg gcaaggttcc
     3241 aattccagtg tgcgctccat aagcaaccga aaaagaataa ccttttccta aatattctgt
     3301 tgctaataat gaagttttga aatcttgacc cattccaccc aaatcttctg gaacagttaa
     3361 gcccaataaa cctaattcgc ccgctttttc taacaaggaa accatcaaac catcttccat
     3421 ggcatcaatt ctatccaaat taggggtaac ttcttgagta ataaaatcgt cgcacatttg
     3481 agcaatcatt ctttgctctt ctgtccattc ctctggtgta aagatttcgt gtggcaaggt
     3541 gtctttaatt aaaaattcgc ctcctttaat tgtgtttttt tctgacatat atcttagttt
     3601 tattcgtttt caattcgcta aagttaataa aagtttttta atattatgca tgcatactat
     3661 attttatttg gtataatttt agctttttta agaattcgat aatagattta cacattcaac
     3721 gttctagaat tatgcggtat tttgttttta ttgttcttgt ttttcaactg attggtgctt
     3781 cggcacaaat gactttttcc accctcaagc atcaatttgg tgatttgcag agttatgaca
     3841 atcggtttgt ggacatcacc ataaccaaca aaggacccaa agacggttac attctcagcg
     3901 tgcgtaagcc tagtgaagta gtgtacattc aaaaccgtgc gtttgtgcaa aaagacagct
     3961 ctctcacctt gcgatttcag gtcaacccgc aacaaaaagg aaaatttagc tataccatac
     4021 aaatttttac cagcgataaa gccgaagcac atcaattggt gttgagcgga aacttggtag
     4081 aactagaaca aaactctaat tcttatttaa cggcttgtcc cgacttcaat acgcatcccg
     4141 caggaaaaaa ccccaatcaa tttgatttaa ttgttgtaac aattgataaa aacacgcgtc
     4201 aggaattgga acaatccaaa gtgagcatgt tgcaagatgg attggcgttg tggacagaaa
     4261 aaacagataa atcggggcgc ataaaaaaat cgggcagcat tggattggct tatttttatg
     4321 ccacacacac caattattta ccagcagaaa aaggagcttt tgtgagcaac gaacgcaaca
     4381 aagtaattat tgaactggag ccaattgcgc agccagaacc tgttgttgaa cccgtgccag
     4441 aaattgcaca acaaacacca gaacctccca aacaggaacc gccaacaggg caaaatcctc
     4501 ccgaacaagt ggtgatagaa attgaagaaa cgcagcctcc aattgcatca agcgagagca
     4561 ttcctgcgtt ggattccatt agcgacgata attttgacag caatttattt gctccagtaa
     4621 acgtggtttt tgtgatggat gtttcttcgt caatgaacca agccaacaaa atggaattga
     4681 tgaaatattc catcaatcaa ttgtctgaaa tgatacgcca agaagataaa attgcgttgg
     4741 ttacttatgc ttcgcaagca aaagtgttgt tgccgccaac ctctggtgtt gacaaagagg
     4801 cggtgcaaga gcaagtaaaa ttgttgcgtg ccggcggaat gactgctggt ggcgaaggta
     4861 tcaaattggg gttggaacaa gctagaaaag cctacatcaa aggcggtgtc aaccatgtat
     4921 ttgtaattac cgatggcgca tttaaccaaa aacaatacaa ctacaaaaaa ttgttgaaga
     4981 aattcaccaa acaaggcatt cagctttcgg tagtaggtgt tttaaacgat gcgttggccc
     5041 gcaaatccat gaaagaaatt gcacaattgg ggcatggaga ttacatttcg gttgccaaat
     5101 tagctgatgc acaaaaaaat atccgtcaag ctgttcgtaa aatggcttat aaacaaaaat
     5161 gatttttgta catttgcaat ctaaattttt gtgataacat ttaaaattaa gataaaatga
     5221 gttttaacgc atggcatgat gtgtcttttg gagacgaagc accacaagtt gtaaaaggaa
     5281 ttattgaaat accaaaaaac agtagagcaa aatacgaatt ggacaaagat tctggtttga
     5341 tttgcttgga tagagttatt gcatcgccaa tgtcatatcc tgcaaactac gggtttattc
     5401 cacaaacata ttgcgacgac aaagatccat tggatatttt ggtgttgaca gacgtagaaa
     5461 tggttccgtt atgtttggtt gaagcaaaaa taataggtgt aatgcaaatg attgatggtg
     5521 gcgaaatgga cgacaaaatt attgctgttg caaaaggcga taaaaactgg aaccacatca
     5581 acgatatttc tgaattgaat ccgcacattt tggatgaaat ccgcgttttc tttgaagatt
     5641 ataaaaaatt ggaaaagaaa tcaacagaag taaaaggatt tttgaaccgt gaagaagcaa
     5701 ttcgcatttt tgaaaaaagc gttgttgact acaaagaaaa atttgccgtt aaagcataat
     5761 aatttttgaa aataacaagt gtccgttttt cgatttatta tgcgaaaaac ggattctttt
     5821 ttaatagcat ttaaaacaaa attaacgtga aaaatttcat tcaagaagta agttggagag
     5881 gaatgctcca cgatgtaatg cccgaaacag aagaacattt gttggaacag atgcgcacgg
     5941 cttatgtcgg aatagaccca acagctgatt cattgcacgt tggtcacttg gtaggtgtga
     6001 tgttgctcaa acattttcaa aacgcagggc ataaaccatt agcgttgatt ggtggtgcaa
     6061 caggtatgat tggtgaccca tccgggaaat caaacgagcg caatttgttg gacgaagcaa
     6121 ctttgaaaca caaccaagcg tgcattcaaa aacaattggc taaattttta gattttgaat
     6181 caaacgccga aaatgctgcc gaaatggtaa acaactacga ttggatgaag aatttttcat
     6241 ttttagactt cattcgcgat gttggaaaac acattacggt gaactacatg atggcaaaag
     6301 attctgttaa aaaacgattg aacggcgaag ccaacgatgg catgagtttc acagaattca
     6361 cctaccaatt ggtgcaaggc tacgactttt tacacttgta taaaactaaa aattgcacgt
     6421 tgcaaatggg tggttctgac caatggggaa acatcacaac aggaacagaa ttgattcgta
     6481 gaattggtgg cggcaaagct tttgcgttaa cttgcccctt gattaccaag gcagatggaa
     6541 ctaaatttgg gaaatccgaa aaaggaaaca tttggttgga cgaagaaaaa acaacaccgt
     6601 atgaatttta ccaattttgg ttgaacactt cggatgaaga tgctgccaaa tacatcaaaa
     6661 tttttacttt cagaagcaaa gaagaaattg aagcattgat tcaagaacat caagaagcgc
     6721 cacatttacg tgtgttgcaa aaagccttgg cagaagattt aaccatcacc gttcacagtg
     6781 agcaagcgtt aaaaacagcc attgctgctt ctaatatttt gtttggaaaa tcaacctcag
     6841 acgatttaaa acaattgtct gaaaaagatt tcttctcggt gtttgctggt gtaccgcaag
     6901 ctactatttc aaaagaagag tttaaaagct taaccattgt agatgcattg gccggaaaaa
     6961 cgggctttct tgcatccaac ggagaggcaa aacgtgaatt gaaagcaaat gcagtagcag
     7021 ttaacaaaga aaaagtggac gaaaacttta ctttttcaga cgaacacctc atcaatggaa
     7081 aatacatttt gattggaaaa ggaaaaaaga acaactacat aataattgtg gagtaaatct
     7141 tttgtgactt caattacaaa aatttgttaa atcttgttat tgtgcttaaa aagtattaaa
     7201 ttagtaccac atttaaaacg aataatcatg aaaaaattag cattagcttt aacattagta
     7261 tttgcaacag gttatatttc taacgcagtt tacgctgcaa catctacaaa ctacacagtt
     7321 ttccaagatg gtaacaagaa aaaaagcaac agcaaaaaat catgtcactc aacttcatca
     7381 gacacttcta aaaagtcttg ttcaggtgaa gcatctaaaa aatcttgctg ttcagctgaa
     7441 aagaaataat aacttttcat aagaattgaa gaaaaccacc ttattttagg tggttttttt
     7501 gtttgaaaca caactacaca tggaaaaagt acttgaaatt gtaaatctta ccaaaaaata
     7561 caaaaactta acagcggttg acaacctcaa cctatctgtt gaaaaaggaa atatttatgg
     7621 tttattagga cccaatggct ctggaaaatc gactactttg ggggttattc tcaacgttgt
     7681 taatgctact tcgggcagtt ggacttggtt caatgaaaca cccaccacac aatcgttgca
     7741 acgcataggc gccattattg agcgc
//
LOCUS       DAOQVQ010000060         7670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10530_length_7670_cov_4.352200, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000060 DAOQVQ010000000
VERSION     DAOQVQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10530_length_7670_cov_4.352200"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="PKN22_03110"
     CDS             <1..1803
                     /locus_tag="PKN22_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNS41724.1"
                     /translation="GLPATWTQTTLATDGGWKNGTAATLSSDYVTFPDLNGTKFMGTN
                     DDACNCNKSADRLISPSMDLTAAGGNPFLQIDYMFVKGSYQGATEAGTIEISTNGGTT
                     WTVLKTLTGLQAFEWRTDLIDLSAYASSNDVKISFLYKDNGGWLFGYGVDNFKVYVPE
                     PNEAVLVSASVNRYSLTNVNNNITLSVKNMGSNPITSLDIEWNDGTGHAATISTNIAV
                     GATANVTHTTPVNYATAQTKNIAVNITQVNGGTDPDMSNNSAATIFTTISQSPTKFPV
                     IEEGTGTWCGWCPRGAVAMDYMYQNYPDFIGVAVHNGDPMTVAAYDAASDFSGFPSSH
                     IDRALRDQDASTTDFENGYNARKNVKTPGKVSAVVTSNGNAVQIAATATIYTNSATED
                     YRMGVIITEDNVTGTTTGYNQANYYAGGGSGAMGGYESLPNPVLAANMVYNHVGRALI
                     GGYDGQAGSIPAPVVDGQAVNYTFNYTVPSTSVKNNMTAIVVLIDNTTHEILNAHKVS
                     LGTVSVAEIETIGLEVYPNPATDKINVTFEAKGGDYAIQLTDLQGRVVLTETHSNLSG
                     SQNFEINTSKLTAGNYLISVSQNGGSFTKMIAIQ"
     gene            1912..5250
                     /gene="mfd"
                     /locus_tag="PKN22_03115"
     CDS             1912..5250
                     /gene="mfd"
                     /locus_tag="PKN22_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNS41725.1"
                     /translation="MTNKEFLSRFEQLPLVEKTAQQLQNHESRIHWKGLAGSASSICS
                     AAVCSQVPGNHLFILSDKEEAAYFLNNIEGLFPDNNNILFYPASFRTPYHFEEVDNAN
                     VVARAEVLDKINSGKNTWIITYPEALFEKVPTQKNLTKNSYKVEVGKTYSIDFLKEVL
                     LENHFEYVDFVYEPGHFSIRGGIVDVFSYSNDFPYRIEFFGDDVESIRTFDASTQLSI
                     NTHQFFTIVPNVQGRMVSEGNGSFLEFIGKNTTIWIASCDKIKETLKSEYKKAVAIHS
                     ELPNTVKHTIPSELYVDDTSFLGELNAFSTIELGIDTHFESHFTATFDFLPQPSFNKN
                     FELLRDDLIARKKNGNEVLVFSNQPKQLERLEQILEDISAEEKPTFTPMNFALSEGFI
                     SPSNQLVCYTDHQLFERYHRFKLKEGFKQTQQALTLKEIYNLQKGDYITHIDHGIGQF
                     SGLETIDVNGKPQEAIRLVYKDGDVLYVGIHSLHRISKFTGKEGTIPKVNKLGTIAWT
                     TLKNKTKKKIKELAYDLIQLYAERKSKPGFQFSPDSYLQNELEVSFMYEDTPDQLKAT
                     LAVKEDMEKPIPMDRLVCGDVGFGKTEVAIRAAFKAVGDNKQVAVLVPTTILSLQHYR
                     SFKERLKDFPCRVDYINRFKSAKQISETLKDLKEGKIDILIGTHAIASDRVKFKDLGL
                     IIIDEEQKFGVSVKDKLKTMRATVDTLTLTATPIPRTLQFSLMGARDLSIINTPPPNR
                     QPIITEIIHFNEEAIRDAVAYEISRGGQVYFVHNRLANIKEVAGMLQRLCPGIRIAIG
                     HGQMDGKELEKIMMGFINYEYDVLLATTIIESGIDIPNANTIIINNAHNFGLSDLHQL
                     RGRVGRSNKKAFCYLISQKSSLLTTEARKRLEALVQFSDLGAGFNIAMKDLDIRGAGN
                     LLGGEQSGFIAEIGFEMYQKILNEAIEELKESEFKELFESRNTDSPLQFVQDTVLETD
                     LEIRIPDTYVNNVAERLSLYQEMSTLKNTAELKEFEKHLIDRFGPTPQEVIDLFQSFE
                     LRWLAQTIGLEKLVIKGGKMIGSFISNPQSPYYESPLFTTIIKAIQANSGTFRMNEKN
                     DKLRIISENIHSLNEAYETLSLLQ"
     gene            complement(5276..7018)
                     /locus_tag="PKN22_03120"
     CDS             complement(5276..7018)
                     /locus_tag="PKN22_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNS41726.1"
                     /translation="MKLFLIGIFLIAFSSFAQLNDVKIEVKDGEKVYVHTVQKGNTLW
                     GLHKMYEVPVEKIVEKNPGIEKGLQEGQQVYIPVPVITETKTHTVVAKETLYSIAKQY
                     DVDVKDLEKWNPECVNGLKVGQQLKIITSTYATGEKANVVEKKENEPIKTAAPIKITF
                     NDSIVEHTVANGETLYSISKRYMVAVDEIVAFNNKKSNVVKPGEVVKIPLKKENISKV
                     EVRPVPVKVIDLPTDSKTSHTKKAEYRVAILLPFMLDKGPGYSERISIMSAEFLMGAQ
                     MALDSLEQLGLRAKVYVYDIENNESKVKQILAKPEMSTMDLVIGPLNKNLAPIIANWC
                     LQRKVKMVCPVNVDTKILQNNPYVYASMGSDITLMKGLAQFIALKFNGDNVLLVRPTL
                     AKDSSLYQAFRSEYKRIAKNGKLIETTPDNLKQFVSSNMKLAIVYPTNDPKSAANFMN
                     EIGKLGTKFGPSSYIFGTDAWSDIESINALQKNNYRISMPSSMDLNYNYDRTKYYHRK
                     YRAKYGSDFTKVAIQGYDVTFNYCYELLMGKEAGQLIMNNFDSKQVGGGHGYENKNVE
                     ILSNQNFDLINISK"
     gene            complement(7064..>7670)
                     /locus_tag="PKN22_03125"
     CDS             complement(7064..>7670)
                     /locus_tag="PKN22_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNS41727.1"
                     /translation="LIEGATWLGQGTIYPDVIESVSVNGGPSKTIKSHHNVGGLPDYM
                     KLQVVEPLRLLFKDEVRRVGKSMNMDDTILQRHPFPGPGLGIRILGEVTADKVRILQE
                     VDYIFISSLKEDRLYGDVWQAGAIFLPVQSVGVMGDERTYENAVALRAVSSTDGMTAD
                     WVHLPYEFLAKVSNKIINQVKGVNRVTYDISSKPPATIEWE"
BASE COUNT         2443 a         1468 c         1411 g         2348 t
ORIGIN      
        1 ggtttgcctg caacatggac tcaaactact ttggcaacag atggtggttg gaaaaacgga
       61 actgctgcaa cattgtcaag tgattatgta acttttccag atttgaatgg aactaagttc
      121 atggggacaa atgacgacgc ttgtaactgt aataaaagtg ccgatcgttt aatttctcca
      181 tccatggatt taactgctgc aggaggtaat ccatttttac aaattgatta tatgttcgtt
      241 aaaggttctt accaaggtgc aactgaagca ggaacaattg aaatatcaac aaatggaggt
      301 acaacttgga cagtgttaaa aactttaaca ggcttgcaag catttgaatg gagaacagat
      361 ttaattgatt taagtgctta tgcttcaagt aacgatgtca aaatttcatt cttatacaaa
      421 gacaacggcg gttggttgtt tggatatggt gtagataact ttaaggtata cgtaccagaa
      481 ccaaatgaag ctgtattggt gagtgcatca gttaatcgtt attctttaac aaacgttaat
      541 aacaatatta cattaagtgt gaaaaacatg gggtctaacc ctatcacatc tttggatatt
      601 gagtggaacg acggaacagg gcacgcagca acaattagta caaacattgc tgttggtgca
      661 actgccaatg taactcacac aacacctgta aactatgcaa cagcacaaac aaaaaatatt
      721 gctgtaaaca ttacacaagt aaatggtgga acggatccag acatgtctaa caacagtgcg
      781 gcaacaattt tcactactat ttctcaatca cctacaaaat tcccagtaat tgaagaagga
      841 acaggtacat ggtgtggatg gtgtcctcgt ggtgcagtag caatggatta tatgtatcaa
      901 aattacccag attttattgg tgttgctgtt cacaatggtg acccaatgac tgtagctgct
      961 tatgacgctg cttctgattt ttctggtttc ccatcttctc acattgatag agcattgaga
     1021 gatcaagatg cttcaacaac agattttgaa aatggataca atgcaagaaa aaatgtaaaa
     1081 actccaggta aagtttctgc agttgttaca tctaatggaa atgcggtaca aattgctgca
     1141 acagcaacta tctatacaaa ctctgcaaca gaagattaca gaatgggtgt aattattaca
     1201 gaagacaatg ttactggaac aacaactggt tataatcaag caaattacta cgctggtggt
     1261 ggtagcggtg caatgggtgg atatgaaagt ttgccaaacc ctgttcttgc tgctaacatg
     1321 gtttacaacc acgtgggaag agctttaatt ggtggatatg atggtcaagc gggttctatc
     1381 cctgctccag tagttgacgg acaagcagta aattacacgt ttaactatac tgttccttca
     1441 acttcagtta aaaataacat gactgcaatt gttgttttaa ttgacaacac aacacatgaa
     1501 attttaaatg cacacaaagt ttctttagga actgttagtg tagcagaaat tgaaactatt
     1561 ggattagaag tttatccaaa tcctgcaact gataaaatca acgttacttt tgaagcaaaa
     1621 ggtggtgatt atgcaattca gttgacagat ttacaaggaa gagtagtgtt aactgaaaca
     1681 cattctaact tgtctggaag ccaaaacttt gaaatcaaca caagcaaatt aactgctgga
     1741 aactatttaa tcagtgtttc tcaaaatggt ggttcattta caaaaatgat tgctatccaa
     1801 taatagaaat taattttctt gacagaaagg ggttcagttt tgaatccctt tctttttttg
     1861 ccttatcttc actatctttg taccctattt taagcaatca tcttttcgtt aatgacaaat
     1921 aaggaatttc tctctcgttt tgaacaatta ccactggtag aaaaaacggc tcaacaactg
     1981 caaaaccatg aatcgcgcat tcattggaag ggattggcag gaagtgcgtc ttctatttgt
     2041 tctgctgcgg tttgctcaca agttcctggc aatcatttat ttattctaag cgacaaagaa
     2101 gaagctgctt attttttaaa caacattgaa gggttgtttc ctgataataa caacattctc
     2161 ttctatcctg catcgtttag aacaccctat cattttgaag aggtggacaa tgcaaatgtt
     2221 gtggcacgtg ccgaagtttt ggataaaatc aattcgggaa aaaatacgtg gattatcacc
     2281 tatcccgagg cattgtttga aaaagtgccc actcaaaaaa acttaactaa aaattcgtat
     2341 aaagtagaag ttgggaaaac ctattccatt gattttttga aggaagtatt gctcgaaaat
     2401 cattttgaat acgttgactt tgtatacgaa cctggtcatt tttccattcg aggaggaata
     2461 gttgatgttt tttcatattc caatgatttt ccatatcgta tagaattttt tggcgatgat
     2521 gtggaatcca tccgcacctt tgatgcatca acacaacttt caataaatac acaccagttt
     2581 tttaccattg ttcccaacgt gcaagggcgt atggtgagtg aaggtaatgg tagtttctta
     2641 gaattcatcg gaaaaaacac caccatttgg atagcatcgt gcgataaaat taaagaaaca
     2701 cttaaatcgg aatacaaaaa agcagttgcc attcattccg aattgcccaa tacggtgaaa
     2761 cacaccattc cttctgaatt gtatgtggat gatacatcgt ttttaggtga attaaacgca
     2821 ttttcaacca ttgaattggg aattgacacg cattttgaga gccactttac tgcaacgttt
     2881 gattttctgc cacaaccctc gttcaacaaa aactttgaat tgttgcgcga cgacctcatc
     2941 gcacgtaaaa aaaatggcaa tgaagtgctt gttttttcaa atcaacccaa gcaattggaa
     3001 cggttggaac aaattctgga agatatttct gcagaagaaa aaccaacctt tacaccaatg
     3061 aatttcgcgt tgagtgaagg atttatctcg ccctctaatc aattggtttg ctataccgac
     3121 catcaacttt ttgaacgcta tcaccgcttt aaactcaaag aaggatttaa gcaaacgcaa
     3181 caagcattaa cactcaaaga aatttacaac ctccaaaaag gcgattacat aacacacatt
     3241 gatcatggaa ttgggcagtt ttctggctta gaaacgattg atgtcaacgg aaaaccacaa
     3301 gaggctattc gtttggttta taaagatggc gatgttttat atgttggcat tcactccttg
     3361 caccgcattt ctaaatttac aggcaaagaa ggcactattc caaaagttaa taaacttgga
     3421 accattgctt ggaccactct gaaaaacaaa accaaaaaga aaattaaaga gttggcctac
     3481 gaccttattc aactttatgc cgagcgtaaa agcaagcctg gttttcaatt ttctcccgat
     3541 tcttatttgc aaaacgaatt ggaagtttcg tttatgtatg aggacactcc cgaccaattg
     3601 aaggcaactt tggctgtaaa agaagacatg gaaaaaccca taccgatgga tcgtttggta
     3661 tgtggcgatg ttggctttgg aaaaacagaa gtggccatac gagcagcatt taaagccgtg
     3721 ggcgataaca aacaagtggc tgttttggtg ccaacaacta ttctttcgct tcaacattat
     3781 agaagtttta aagaacgttt aaaagacttt ccatgccgtg ttgactatat caatcgattt
     3841 aagtcggcaa aacaaatttc agaaacgcta aaagacctca aagaaggaaa aattgatatt
     3901 ttaattggaa cacacgccat agcttccgac agagtaaagt tcaaagattt ggggttgatt
     3961 atcattgacg aggaacaaaa atttggcgtt tcggtaaaag ataagctcaa aaccatgcgt
     4021 gccacagttg atacgctaac attaacggca acacctattc cacgaacgct tcaattttct
     4081 ttgatgggtg cccgcgattt gagcattatc aacacacctc cacccaatcg tcaaccaatt
     4141 attacagaaa tcattcattt caacgaagaa gccattcgcg atgcggttgc ctatgaaatt
     4201 agtcgtggtg gacaagtgta ttttgtgcac aaccgtttgg ccaacataaa ggaagttgcg
     4261 ggcatgttgc aacgtctttg tccgggcata cgcattgcaa tagggcacgg acaaatggac
     4321 ggtaaggaat tggaaaaaat catgatgggc ttcatcaatt acgaatacga cgtgttgttg
     4381 gctactacca tcattgagtc agggattgac attccgaatg cgaataccat tatcataaac
     4441 aatgcgcaca attttgggtt gagcgatttg caccaattaa gaggtcgcgt tggtcgttcc
     4501 aacaaaaaag cattctgtta tttaatttcc caaaaatcat cgttgctaac tacagaagca
     4561 cgcaaacgct tggaagcatt ggttcaattc tcagatttag gagctggatt taacattgca
     4621 atgaaagatt tagacattcg cggagctgga aatttattag gtggtgagca aagtggtttc
     4681 attgccgaaa ttggctttga aatgtaccag aaaatattga acgaagccat tgaagaattg
     4741 aaagaaagcg agtttaaaga actttttgaa agtagaaata ccgattcgcc actacaattt
     4801 gtgcaagata ctgttttgga aaccgattta gaaattcgca ttccagacac ctatgtaaac
     4861 aacgttgccg agcgtttgag cttgtatcaa gaaatgagca cgctcaaaaa tacggctgag
     4921 ttaaaagaat ttgaaaaaca tttaattgac cgttttggtc caacaccaca agaagtaatt
     4981 gatttatttc aatcgtttga attgcgttgg ttggcacaaa ccattggctt agaaaaattg
     5041 gttatcaaag gtggaaaaat gattggtagt tttatttcca accctcaaag tccctattat
     5101 gaatcgccgt tgtttaccac tattatcaag gctattcaag caaattctgg aacttttagg
     5161 atgaatgaga aaaacgataa attgcgcata atttccgaaa acattcacag cctaaatgaa
     5221 gcctacgaaa cattgtcgtt gcttcaatag aaaaacaatt ttaagacgat tttgtttatt
     5281 tgctgatgtt aatcaaatca aagttttgat tggataggat ttccacgttt ttgttttcat
     5341 atccgtgacc tccacctact tgctttgaat caaaattgtt catgataagt tgaccagctt
     5401 ctttgcccat caacaattca taacaatagt taaacgtaac gtcgtatcct tgtatcgcca
     5461 cttttgtgaa atctgaaccg tattttgcac ggtattttcg atggtaatac ttcgttctat
     5521 cgtagttgta attcaaatcc atagatgatg gcatagaaat acgataattg tttttttgca
     5581 acgcattgat actttcaata tccgaccaag catctgttcc aaaaatataa gaacttggac
     5641 caaatttagt tcccaatttc ccaatttcat tcataaaatt agccgctgat tttggatcgt
     5701 tggttggata aacaattgcc aacttcatgt tgctcgatac aaactgtttc aaattatcgg
     5761 gtgtagtttc aatcaatttg ccgtttttag caatgcgttt gtactctgag cggaatgctt
     5821 ggtacaacga tgagtctttt gcaagtgttg gacgcaccaa caatacatta tcgccattaa
     5881 attttaaagc aatgaattgt gccaaccctt tcattagtgt aatgtcactt cccatagacg
     5941 catacacata cgggttgttt tgcaaaattt tagtatccac attaactgga cacaccattt
     6001 ttactttacg ctgcaaacac caattggcaa taataggagc aagatttttg ttcaaaggtc
     6061 caatcaccaa atccattgta ctcatttctg gttttgccaa aatttgtttc acttttgact
     6121 cgttgttctc aatgtcatac acataaactt tggcacgcaa tcctaattgt tccaaactat
     6181 caagggccat ttgtgcaccc attaaaaact cagccgacat gatggaaatg cgctcggaat
     6241 aacccggacc tttatccaac ataaaaggca ataaaatagc tacacgatat tctgctttct
     6301 tggtgtgcga agttttgcta tcagttggta aatcaataac ttttactgga actgggcgaa
     6361 cctcaacttt tgaaatattt tcttttttca acggtatttt tacaacctca ccaggtttta
     6421 caacattgga ttttttgttg ttaaatgcca caatttcatc aaccgcaacc atgtagcgtt
     6481 ttgaaatgct atacaacgtt tcaccattag caacggtatg ctccacgatg gaatcattga
     6541 acgtaatttt tattggtgca gccgttttta ttggctcgtt ttcctttttt tctacaacat
     6601 tagctttttc acccgttgcg taggtagaag tgataatttt taattgctgt ccaaccttca
     6661 acccattgac acattctgga ttccattttt ccaaatcttt aacgtccaca tcgtattgtt
     6721 ttgcaataga atacaacgtt tcttttgcta caacagtgtg tgttttggtt tcagtaatta
     6781 caggaactgg aatataaact tgctgacctt cttgcaaacc tttttcaata ccgggatttt
     6841 tttctacaat tttttctaca ggtacttcat acatcttgtg taaaccccac aacgtgttgc
     6901 ctttttgaac agtatgaaca tacacttttt caccatcttt tacttcaatt tttacatcgt
     6961 ttaattgagc aaaagaagaa aatgcaatta gaaaaatacc aataagaaat agtttcatgt
     7021 gttgaatttt tttattgaac ctaacaatca atgtgccaaa tggttattcc cactcaattg
     7081 ttgcaggtgg tttagagcta atgtcataag taactctgtt cacacctttc acttggttga
     7141 tgattttatt agaaactttt gctaaaaatt catacggtaa atgtacccaa tcggcagtca
     7201 taccatccgt tgatgaaaca gcacgcaatg caaccgcatt ttcatacgtt ctttcgtcgc
     7261 ccattacgcc caccgattga actggtaaga aaattgcacc cgcttgccaa acatcgccat
     7321 acaaacgatc ttctttcaag cttgagatga aaatgtaatc cacttcttgc aaaatgcgca
     7381 ctttatcggc tgtaacttcg cccaaaatac ggattcctaa acctggtcca gggaaaggat
     7441 gacgttgaag aatggtatcg tccatgttca tagatttacc aacacgtctc acctcgtctt
     7501 taaacaacaa acgcaacggt tctaccactt gcaacttcat ataatcgggt aaaccaccaa
     7561 cgttgtggtg cgattttata gtttttgaag gaccaccatt tacagaaacc gattcaatga
     7621 catcagggta aatagttcct tgacccaacc aagttgcgcc ttcaattaat
//
LOCUS       DAOQVQ010000061         7567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10731_length_7567_cov_3.951012, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000061 DAOQVQ010000000
VERSION     DAOQVQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10731_length_7567_cov_3.951012"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKN22_03130"
     CDS             <1..946
                     /locus_tag="PKN22_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41728.1"
                     /translation="RIAPIDSKDCDNVCGRFKDGSKIWIALVDATENSFAITKDGGSN
                     WEQVATGGIVHADPTWDSLLLVSSGRVYWSSSAVGPSTKIYSADLNEILADDYAPTIT
                     EVLTAGLFDPKGIVATDSYVYVVGQDETGFDGEVYRINKTSQTTELIYTNSMGITAVS
                     NLNDDVSLIGTREGELYLSDISGLYYFIGAIDGDDSEIYSLHMFDERRWVASNQNGIH
                     VTDDRGTSWSRTLSMSSINSTSDIFKMSWYDNLVGYVQIGNLIFRSIDAGSSWKKIYT
                     GVSVDEAQSIVVNPNNPNLFFSVANIDSNTSYVVKGYV"
     gene            993..1235
                     /locus_tag="PKN22_03135"
     CDS             993..1235
                     /locus_tag="PKN22_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41729.1"
                     /translation="MNKFYQVENNNAEKVVFIPDGNIDHISAGIEIKFIKNIPMFIEL
                     LNKNYGILLLHYQSESKVDIQRLITRTVREYLESIK"
     gene            1254..2708
                     /locus_tag="PKN22_03140"
     CDS             1254..2708
                     /locus_tag="PKN22_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41730.1"
                     /translation="MASTLARNDLARMFLFESGVLNAVPVWRYYPCAGVDGLSQDFGE
                     VTRIECPDPYRYGQFIEVDSVPGEIGRLTTTLTTRLSRNERSIFRQLATKGCAFDLHL
                     HFGLCARPTDFNSFDKVMIFENVSVTSYGTDPLVALTSGDRAEINETADISVGNYYEV
                     LPLAYAETDTVNVALASAIIGAVYADVQNCGIGCNDASSGCEVMFAIDADGDLIQSEN
                     GGTTWVLNNDAWGTATIGLGYLNNYVWTYNTATSAIFSATKEDALAQVAPASTAASAD
                     VEVPADLANGFAYGLVVGSNGFVGVLRSPASGFDVTYTGAFTTEDYGVVAFNPYTDIA
                     LIGGTNNAILYTPNGEDFETVTGPTAQAAVTVTAVLPVSEKKWLIGYADGDVYTTDNA
                     GGSWTLVAVPGGANPVVDLAMSTTHVLWMAKNNVFYQSIDGGASWLKQPNSKKAFPTS
                     SALNVILPCPYDVNKITVFGADGAAGFHAIGTPA"
     gene            2757..3260
                     /locus_tag="PKN22_03145"
     CDS             2757..3260
                     /locus_tag="PKN22_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41731.1"
                     /translation="MNLRQVVGTFTRPNDTDAYAIGDVVANSTTAPVAISFANASRSN
                     DGSNTIIDAMLIDSAAQATKGQFELWLFKTAPTMDNDNAAFTPTDAELLNLVGIIDFT
                     AAPRVGDATAGAGGNAVYYGKASNGSFEPKFRTKVASRTLYGVLVARNAYTPVAQEVF
                     TIVLTLD"
     gene            complement(3410..3847)
                     /locus_tag="PKN22_03150"
     CDS             complement(3410..3847)
                     /locus_tag="PKN22_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41732.1"
                     /translation="MYKIILPLVFVVIFSLREQAELLWVNDPANQRELMNKHAALMNA
                     AQWKADYIAATGDFAHCTKNGECSNKTIKRFGCITGYDENKNFVESLVWGTNVPDIAY
                     ENLLMSSDHVQHLHATNEFFRSQTDYGVGHADLIFVFISAKCE"
     gene            complement(3897..4151)
                     /locus_tag="PKN22_03155"
     CDS             complement(3897..4151)
                     /locus_tag="PKN22_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41733.1"
                     /translation="MYAIYKKDNKFYIIKIMSVVGEFVYLNIPGYDTFLDTRSDIILG
                     KYGVFQTIILEEEVEIFETYIREFEYEYVYNNFEEIFKIT"
     gene            4237..5259
                     /locus_tag="PKN22_03160"
     CDS             4237..5259
                     /locus_tag="PKN22_03160"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS41734.1"
                     /translation="MANCCSNSFYSATLNTPDCSIECLSLATPGAIFVGANGDIFILT
                     GTDPCNLTHWFESGSCCINFATLTESLSVCCNEEISLTSSDGSIGIDIITGGIDFVSD
                     FVFSFSDDYTDEFDVTHTSDVRLSDANGVTVEITSPGVFQISGNSYSGSIAPTFTPDH
                     STIINFYYNTATNQLYAWDSVESAWILLTHQPLPVAIYTFTKSAMAASFDGNASTSTQ
                     VGTTLTYLWSGSGPAAVVFATPTAITTTATFTVDGSYVITLTVTDSNGAVKAYSETIL
                     IEPKDRCDIKFEIPGIAFVDPDNPTESEVNTWITANGPFNNGTLLYYVGGGDSLSPDF
                     IWYYTC"
     gene            5261..6775
                     /locus_tag="PKN22_03165"
     CDS             5261..6775
                     /locus_tag="PKN22_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41735.1"
                     /translation="MRKLRNNKIVINDKTRLFIQSLNSNNCNKEYSYHSCISLGDVSI
                     PKVALQKHYISSSDRYNEFDILTTTEGEKQEGSSSLTTKFTSEASILKKYYNSNCSFD
                     MQVHIGECDNASNFSIFDKAIIFKNVVITDYSINSLMASAQADRDVVTESVSFTFDEM
                     IEISLPNLQPISHTAISAGPIIDGFTFCNDATCSLCEGQIGRYFVQLVECGVECRLVR
                     IIYTLDEGVTWNRKDISICEDLNCDQLINTNLVQNTTHYYSLNLSTFNETSLFQILEN
                     STSIILKSLLYGSKLYQSHTSFGTIFYVGSNGKLFYSINGLIYRLTNTLLLISDNLFS
                     ISSLDGYNFVIGSENGKVYLGNIDGEFKVAYLTNGGNVNAIAMTSDCSFVASNGENGG
                     FVYTNGKAAKMKNIFGAITNFTFFNNDIGYATSHTSSGVYFWQTVDGGRSWQRLSKTL
                     TSNYVVTTLTICGNDHNKISVAGRKMESNVTVEDVLNKELLWNCIGEGFVLFAT"
     gene            6955..>7567
                     /locus_tag="PKN22_03170"
     CDS             6955..>7567
                     /locus_tag="PKN22_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41736.1"
                     /translation="MIELMLESGIPVIVKQIPNILIFTELNKSNLRVYLPPIYTTDDG
                     FEQENTFSPVYNDAIAIYELTKNNLAFEILLKHAVTFDKRLMSNNKKWQREKNILQSN
                     PLLTIPKEEEIAFLKFYLYGDSYIDKINTTQNALLTEHRVFDTFNAIAITRNGLDIHT
                     VNMKNSVDVDITIHPISIDGLQIVNPLDELECCIETGLEWSKWL"
BASE COUNT         2473 a         1117 c         1347 g         2630 t
ORIGIN      
        1 aagaattgcc ccgattgatt caaaggactg tgataatgtt tgtggtaggt ttaaagatgg
       61 aagcaaaata tggattgcgc tagttgatgc aacagaaaat agcttcgcca taacgaaaga
      121 tggaggtagt aattgggaac aagttgctac tggagggatt gttcacgctg atccaacatg
      181 ggattctctt ttattagttt cttctggcag agtctattgg agttcaagcg cagttggacc
      241 aagtacaaaa atttatagtg cagatttaaa tgaaattctt gcagatgatt atgcgccaac
      301 tataactgaa gtattaacag caggcttgtt tgatcctaaa ggaattgtcg caactgatag
      361 ttatgtttat gtagttgggc aagacgagac aggattcgat ggagaagttt atcgtattaa
      421 taaaacatca caaacaactg aattaattta cactaattct atgggtatta cagcagtttc
      481 taatttgaat gatgatgtat ctttaattgg cactagagaa ggtgaattgt atttatctga
      541 tatttcagga ttatattatt ttattggggc aattgatggt gatgatagtg aaatctattc
      601 gttgcatatg ttcgatgaaa gacgatgggt agcatcaaat caaaatggca ttcatgttac
      661 tgatgatcga ggaacttcgt ggagtagaac gctgtcaatg agtagcatta actctacatc
      721 agatatattt aaaatgtcat ggtatgacaa cttagttggg tatgtacaaa ttggaaatct
      781 tatattccga tcaattgatg ctggatcaag ttggaaaaaa atctatacag gtgtttctgt
      841 agatgaagca caaagtattg tagttaatcc aaacaaccct aatttatttt tctctgttgc
      901 gaatattgat agtaatacta gttacgtagt taaaggatat gtctaaaaat ttaaacttga
      961 taattaatca agtgaagaat aagatatgaa ttatgaataa attctatcaa gtcgaaaata
     1021 ataatgcaga aaaagttgtc tttattcctg acggtaatat tgatcatatt tctgctggta
     1081 tagaaataaa atttattaaa aatataccaa tgtttattga gttattgaat aaaaactatg
     1141 gaattctatt actacattac caaagtgaat ccaaagttga tattcaacgg ttaattactc
     1201 gaacagttcg agaatattta gaaagtatta aataatttta aggagattaa aaaatggcta
     1261 gtactttagc aagaaatgat ttagcacgta tgtttttatt tgaaagcggt gtgctaaacg
     1321 ctgtacctgt atggagatat tatccatgcg caggtgttga tggtctttca caagacttcg
     1381 gtgaagtgac tagaattgaa tgtcctgatc cttacagata cggacaattt attgaagttg
     1441 atagtgttcc aggtgaaatt ggtcgtttga cgacaacttt gacaacacgt ttaagtcgaa
     1501 acgaacgttc aattttcaga caacttgcaa caaaaggttg tgcttttgac cttcacttgc
     1561 attttggttt atgtgccaga ccaacagatt ttaacagctt tgataaggtt atgatctttg
     1621 aaaatgtttc tgttacttct tatggtactg atcctttggt tgcattaact tcaggagata
     1681 gagcggaaat taatgaaact gctgatattt cagtaggtaa ttattacgaa gttcttcctc
     1741 tagcgtatgc agaaactgat accgttaatg ttgcacttgc atctgcaatt atcggtgctg
     1801 tttatgctga tgttcaaaac tgcggtatcg gttgtaatga cgcttcaagt ggttgtgaag
     1861 ttatgtttgc tattgatgct gatggtgatt taatccaatc ggaaaatggc gggacaactt
     1921 gggtattaaa caatgatgct tggggtactg caacaattgg tcttgggtat ttgaataatt
     1981 atgtttggac ctataataca gcaacaagcg caattttcag tgcaacaaaa gaggatgctt
     2041 tagctcaagt cgctcctgca tcaactgctg cttctgctga tgttgaagtc cctgccgacc
     2101 ttgcaaatgg atttgcttat ggtcttgttg ttggctcaaa tggcttcgtt ggtgtgctac
     2161 gttctcctgc aagtggattt gatgtcactt acactggcgc ttttacaact gaagattatg
     2221 gtgttgttgc ttttaatcct tacacagaca tcgctttaat tggtggaaca aacaacgcaa
     2281 ttctttatac accaaatggt gaagattttg aaaccgttac tggaccaact gcacaagctg
     2341 ctgtaactgt gactgctgtt cttcctgttt ctgaaaagaa atggcttatc ggttatgctg
     2401 atggtgatgt ctacacaaca gataacgctg gtggaagttg gacactagtt gctgttcctg
     2461 gtggcgcaaa tcctgttgtt gatcttgcaa tgtcaacaac tcatgttctc tggatggcaa
     2521 agaataatgt attctatcag tcaatcgatg gtggcgcaag ttggctaaaa caaccaaata
     2581 gcaagaaagc tttcccaact tcatctgcgt tgaatgtcat tcttccatgt ccttacgatg
     2641 tgaataagat tactgttttt ggtgctgatg gtgctgctgg tttccatgca attgggacac
     2701 ctgcgtaata aaaagtaagg ggagaaatcc cctttattag tataggagat taaaaaatga
     2761 atttaagaca agttgtaggg acttttacac gtccaaatga tacagatgct tatgctattg
     2821 gtgacgttgt tgctaatagt actacagcac ctgttgcaat tagcttcgct aatgcctcta
     2881 gaagtaacga tggtagtaat acaattattg atgctatgct aatcgatagt gcagcgcagg
     2941 caacaaaagg tcaatttgaa ctttggctgt ttaaaactgc tccaacaatg gataatgata
     3001 acgcagcttt tacaccaact gacgcagaac ttttgaacct cgttggtatt attgatttta
     3061 ctgctgctcc aagagtaggt gacgcaactg ctggtgctgg tggcaatgcg gtttattatg
     3121 gtaaagcttc aaatggtagt tttgaaccta agttcagaac aaaagttgct tcacgtacat
     3181 tgtatggtgt tttagttgca agaaatgctt atactcctgt tgctcaagaa gtttttacaa
     3241 ttgtactaac acttgattga tgacttgaca aagtaaaaag ttatgatata atagctttac
     3301 attctaaata agttttaata caaaactaga tttgattggt ttctaagtga tttagaatga
     3361 agccaaaaac caatcataaa aaacaccctc gtaagagggt gtttttattt tattcacatt
     3421 ttgctgaaat aaaaacaaat attaaatcgg catgaccaac accataatct gtttgcgacc
     3481 tgaaaaattc atttgttgca tgtaaatgtt gaacatggtc tgaagacatt agaagattct
     3541 cgtaagcaat atcaggaaca tttgttcccc aaactaaact ttccacaaaa ttcttatttt
     3601 catcatatcc agttatacaa ccaaatctct ttattgtttt atttgaacat tcaccatttt
     3661 tagtacaatg agcaaaatca cctgtcgctg caatataatc tgctttccat tgtgcagcat
     3721 tcattaaagc tgcatgttta ttcatcaact ctctttggtt agcaggatcg ttcacccata
     3781 gcaattctgc ttgttctctt aatgagaata ttactacaaa aactaatggt aatattattt
     3841 tatacatagt actcctttct agtaaagtat actatgtata aagttttatc tcaatattaa
     3901 gttatcttga atatttcttc aaaattattg taaacgtatt catattcaaa ttctctaatg
     3961 tatgtttcaa atatttcaac ttcttcttct aatataattg tttgaaatac accatacttt
     4021 cctaaaatta tatctgatct agtatctaag aatgtatcat aaccaggaat attaagataa
     4081 acaaattcgc caacaacact cattatttta atgatataaa acttattatc ttttttataa
     4141 attgcataca tattttcctc actgaattgt attaattgat tattaacttc ttttatttta
     4201 ctatagatat aattgtattg atagaaaggt gaaattatgg ctaattgctg ctcaaatagt
     4261 ttttattcag ccacattaaa tacaccagat tgtagcatag aatgcttatc tcttgcaact
     4321 cctggtgcta tatttgttgg tgcgaatggt gatattttta tattaacagg cacagaccca
     4381 tgtaatttaa cacattggtt tgaatctggc tcatgttgta ttaattttgc aacattaaca
     4441 gaatcgttaa gtgtttgttg caatgaagaa atttctttaa caagctcaga cggttcaatt
     4501 ggaattgata ttattactgg tggtattgac tttgtgtctg actttgtatt ctcattctca
     4561 gatgattaca cagatgaatt tgatgttact catacttcag atgttagact atctgatgca
     4621 aatggtgtaa cagtagaaat tacaagtcca ggcgtatttc agattagtgg taatagctac
     4681 tctggttcaa ttgctcctac ttttactcct gatcattcaa caataattaa tttctactat
     4741 aacacagcaa caaatcaact gtatgcatgg gatagtgttg aatcagcatg gattcttcta
     4801 actcatcaac cattaccagt agctatttat actttcacaa aaagtgcaat ggctgcatca
     4861 tttgatggta atgcaagtac aagtacacaa gttggaacaa ctttaactta tttatggtct
     4921 ggtagtggtc ctgctgctgt tgtatttgca acgccaactg ctattacaac tacagctaca
     4981 tttactgttg atggctctta tgttattaca ttaactgtga ctgattctaa tggtgctgtg
     5041 aaagcatata gtgaaactat tctaattgaa ccaaaagata gatgtgatat aaaatttgaa
     5101 atccctggta ttgcttttgt tgatcctgat aacccaactg aatcagaagt taatacgtgg
     5161 attacagcaa atggtccatt taataacggt acattgcttt attatgttgg tggtggtgat
     5221 agtctttctc ctgattttat ttggtattat acttgctaac atgcgtaaat taagaaataa
     5281 taaaatagta atcaatgata aaacaagatt gtttattcaa tctttaaatt caaacaattg
     5341 caataaggaa tattcttatc attcttgtat ttctcttggc gatgtttcta ttccaaaagt
     5401 tgctttacag aagcattata tctcaagttc tgatagatat aatgaatttg atattttaac
     5461 tactacagaa ggagaaaaac aagaaggttc ttcttcatta acaactaaat ttacttcaga
     5521 agcaagcata ctaaaaaaat attataattc taattgttca tttgatatgc aagttcatat
     5581 tggtgaatgt gataacgcaa gcaatttttc tatttttgat aaagcaatta tatttaaaaa
     5641 tgttgttatt acagattatt caattaattc tctaatggca tcagcacaag ctgatagaga
     5701 tgttgttaca gaaagcgtaa gttttacttt tgatgagatg atagaaatat cactgccaaa
     5761 tttacaacca atttctcaca cagcgatttc tgctggtcca atcattgatg gttttacttt
     5821 ctgtaatgat gcaacatgct ctttatgtga agggcaaata ggaagatatt ttgttcaatt
     5881 agttgaatgt ggagtagagt gtaggttagt tagaataatc tatacacttg atgaaggtgt
     5941 tacttggaat agaaaagata ttagtatttg tgaagatttg aattgtgatc aattaattaa
     6001 tactaattta gtacaaaaca caacacatta ttattcttta aatttatcta catttaatga
     6061 aacttcttta tttcagattt tagaaaatag tacatctatt atattaaaat cattattata
     6121 tggctctaag ttatatcaaa gtcatacttc atttggaact atcttttatg ttggttctaa
     6181 tggaaaactt ttttattcta tcaatggatt aatttataga cttacaaata cattgctttt
     6241 aatttccgat aatctctttt caatttcttc tttagatgga tataattttg ttattggtag
     6301 tgaaaatgga aaagtatatt taggaaatat cgatggtgaa tttaaagtag cttatttaac
     6361 aaatggtggc aatgttaatg caattgcaat gacaagtgat tgttcgtttg ttgcgtcaaa
     6421 tggtgagaac ggtggtttcg tttataccaa tggtaaagcg gcaaagatga agaatatatt
     6481 tggggcaatt actaatttta cctttttcaa taacgatatc ggatacgcaa cttcccatac
     6541 ttcatctggt gtttatttct ggcaaacagt tgacggtgga agaagttggc aaagattaag
     6601 taaaacatta actagtaatt atgttgttac aactttaact atttgtggca acgatcataa
     6661 taaaatttct gttgctggca gaaaaatgga aagcaatgta actgttgaag atgtcttaaa
     6721 taaagaatta ttatggaatt gtattggtga aggattcgtt ttatttgcaa cttgacaaat
     6781 aaaaatattg tgatataata gttttatcat taaccactaa aaggtatgct ttggtaatgt
     6841 acgtagccga gagttgatga ctaaacataa attaccacta aagagattat taatttagtc
     6901 tcttttttta ttttccttat tgacaataaa ataatagtat gatattataa agtaatgatt
     6961 gaattaatgt tagaatctgg tattcctgta attgtcaagc aaattccaaa cattcttatt
     7021 tttactgaat taaataaaag taacttaaga gtctatctac ctcctatcta caccacagat
     7081 gatggatttg aacaagaaaa tactttttct cctgtttata atgatgctat tgccatttat
     7141 gaattaacca agaataattt agcatttgaa atattactaa aacatgctgt tacttttgat
     7201 aaaagattaa tgagtaataa taaaaaatgg caaagagaaa agaacatctt acaatcaaat
     7261 ccgcttctta caattccaaa ggaagaggaa attgcatttt taaaatttta tctttatggt
     7321 gacagttata ttgataaaat taatacgacc caaaacgctc tattgaccga acatagagtt
     7381 tttgatacat ttaatgcaat cgcaataaca cgtaatgggc ttgacattca tacagtgaat
     7441 atgaaaaatt cagtcgatgt tgatattaca attcatccaa tttcaattga tgggttacaa
     7501 attgtcaacc cattagatga attagaatgt tgcatcgaaa caggattaga atggtcaaaa
     7561 tggttaa
//
LOCUS       DAOQVQ010000062         7567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10732_length_7567_cov_3.897897, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000062 DAOQVQ010000000
VERSION     DAOQVQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_7567_cov_3.897897"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..791
                     /locus_tag="PKN22_03175"
     CDS             45..791
                     /locus_tag="PKN22_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNS41737.1"
                     /translation="MFNFLGIIPLFSIVGGKDVEVPTHLDAIAGANQWILGFVLFLSF
                     LLLALAKRLDPYIIRVALFSVFTLDTPDNLQKIDARYNSTSFVLLGFLSFLSLWVCLV
                     LFNEQTHFLNQVSIGLLGEKFGSIDDLLLTLIGTVIVLFYFFAGLLISTFVTGERELF
                     STFITQSWVNFISFGIVFFVLALLWLLNSPINPYISQLFLYILLALFLVRMLKILIVA
                     LLNGVSWYYLILYLCTLEVLPILAVFALMA"
     gene            829..1578
                     /locus_tag="PKN22_03180"
     CDS             829..1578
                     /locus_tag="PKN22_03180"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNS41738.1"
                     /translation="MAVKTILVSQPRPETEKSPYFDLAEKYKLKIDFRPFIQVEGIEA
                     QEFRTQKVNIAEHTAVIFTSKIAVDHFFRIAKEVRFDVPDTMKYFCISEAIAYYLQKY
                     VTYRKRKIFFGRQTIDDLVVLMKKHKTEKFLLPCTDVLQDKIPETIKEHKINFSKAVL
                     YRTVAADLSDLEDVYYDMLVFFSPAGVESLLKNFPDFKQNDTIIAAFGPTTAQSLVKN
                     NLRLDVHAPQPDAPSMPAAIELFIKNDLKKK"
     gene            1656..2318
                     /locus_tag="PKN22_03185"
     CDS             1656..2318
                     /locus_tag="PKN22_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNS41739.1"
                     /translation="MKSIVLIVIVAIIGITLFYVNNQPKEAQLPIYNPVDINPEVVDS
                     SLQRVGIGHTIGDFTFTNQNGQTITRKDVDGKVYVVEYFFTTCGSICPIMNKQMQRVQ
                     KKYAQHDDFKILSFTVDPKNDTVAQLKRYAVQHHANDKQWWFLTGDKEQLYALARKSF
                     FVLKPAEAENVGDAGSDFIHTNNFVLIDKKQRIRGYYDGTNPQSVDSLITDIEKLFDS
                     NN"
     gene            complement(2315..2842)
                     /locus_tag="PKN22_03190"
     CDS             complement(2315..2842)
                     /locus_tag="PKN22_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNS41740.1"
                     /translation="MALIKACRGTSPKFGKDCWFAENATIVGDVVMGDECSVWFNAVV
                     RGDVNSIKMGNRVNVQDGAVIHCTYEKTKTVLGNNVSIGHNALVHGCTVEDNVLIGMG
                     SIVMDNCYIKANCIIAAGAVLLENTRVESWSIYAGVPAKKVKTLSPELFEGEIKRIAN
                     SYVKYSSWFKEEQEG"
     gene            complement(2847..4037)
                     /locus_tag="PKN22_03195"
     CDS             complement(2847..4037)
                     /locus_tag="PKN22_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNS41741.1"
                     /translation="MMSRTDDFLNYTGQTNQTPYLIDVERAEGIYITDKNGKKYMDMI
                     SGVSVNNIGHSHPHVIKAVKEQAEKYMHVMVYGEFIQDAHLDFCKKLVGLLPEQLNCA
                     YTVNSGTEANEAALKLAKRVTRRTELVAFRGSYHGSTHGSMSVSGNETKKEPFRPLLP
                     DVRFLQHNNIEQLEQITEKTAGVIIETVQGDAGVRVPTKEFLMALRKRCTEVGALLIF
                     DEIQCGMGRTGKLFAFEHFGVVPDVLTLGKALGGGLPIGVLVSSRKNLWEFSYDPILG
                     HITTFGGNPVIAAAGLACLEIFEKEIDFNEVERLGALLQSIVGQSDEIKAMRRIGMMF
                     AFDMESFERVEKVVKKCLEDGLISFWFLSHPYSFRLSPPLTITEEEVRKAGAIILNAI
                     QSTK"
     gene            4132..4560
                     /locus_tag="PKN22_03200"
     CDS             4132..4560
                     /locus_tag="PKN22_03200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNS41742.1"
                     /translation="MSDLNIVELYKMSNEFGRTIDFEYDFLPNGEVTNKIIIDKKHLA
                     TPVTAHGGVIAALADSTLGIAALAESQKDGNLVSTVEFKINFLKPACLGDVISGFSRV
                     RKVGKKIIFVEADFLNQNEELVATASGTFNQYPFSKVFNL"
     gene            4564..5904
                     /locus_tag="PKN22_03205"
     CDS             4564..5904
                     /locus_tag="PKN22_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41743.1"
                     /translation="MLDFPKDFVNYVENDAHLGADLLTALNSEVPISIRKNPRKTKCS
                     FKAEALVLWYEDVYFLKERPVFTLNPLFHAGTFYPQEAGSMLLDFIIRSLDLPEQPKF
                     LDLCAAPGGKSTLIASFLDDKGLLVANEVINQRAKILKENVSKWGYPNVVVTNNDPSD
                     FERTPNFFDVVVVDAPCSGEGMFRKDVQARNEWSLDNVNLCQSRQKRIVADVWDALNE
                     NGYLIYSTCTFNPHENEENIRWITENFDAELVTIPAPFDFVQGRGGVGIYGIPGRSQT
                     EGFFIAVLQKKSSEGRTTKLGKSQNKGLTKVKDIGELSKWVKTTASTFYEWNATILAI
                     PSAFEKEYLLLQSNLHLIKWGVEVGEIARKGLIPSHDLLLCHSLRLNYPSHEVSEQEA
                     LFFLHGDTFSINGLTHLGMVQLTYQNEPIGWIKNIGNRFNNLYPKEYRIRMNVQ"
     gene            complement(5913..>7567)
                     /locus_tag="PKN22_03210"
     CDS             complement(5913..>7567)
                     /locus_tag="PKN22_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41744.1"
                     /translation="SHNTQAITPTPTVTYIPNTTTLNAKVVVNQPGVYPFILTESNQG
                     CGAMNDTVLITVSQATQTVQTTDLTCFGNQTGIIQIINNDAVSYSFDNGATWETSSTK
                     TGLAAGTYTVWSENQYGCRTSNTVILLEPDALQLTVANDTLICENGTATLTASIGQTN
                     AIYHWGHTTDVAATQLVQPTALTNYTVYAESAAGCLSDTQTIVVSLRNPISAVITTNA
                     AICPGETTTLQVTNIAGGLDPYTVTWNTNQIGTSLQVSPAQTLQYTAIVTDACESTPL
                     VLNTTVTVHPLPTPIISSPSPSICEPAQFELVCNTDPSLIQTVNWFISDGQSFLNQTN
                     VITEPMPAGNYTVEVLIVSPQGCIDSKTFTNFLTVHPLPTADFVWNPNPVKVFSTNVT
                     MNNSSSGATAYNWFFEGASPSVSTLENPSVKYPEGVEGTYPIMLVATTEFGCQDTVVK
                     ELVVLPDVLLYVPNTFTPDGDEFNQNWRVYTEGIDLYNFSLTVYNRWGETVWQSNDAS
                     IAWDGTYKGQVVQEGTYTWVIIAKDRINDNHYKWTGTVNVLK"
BASE COUNT         2257 a         1363 c         1468 g         2479 t
ORIGIN      
        1 ttcaaagtta tttttgttaa agaaacatca taatattcat aaatatgttc aattttttag
       61 gaatcatacc gcttttttca atagtaggag ggaaagatgt tgaagttcct acacatttgg
      121 acgctattgc gggtgcaaat caatggatat taggttttgt tttgttttta agttttttat
      181 tgttggcctt agctaagcga ttagatccat atattattag agttgcctta ttttctgttt
      241 ttaccttaga tactccagat aatttacaaa aaattgacgc acgatataat tcaaccagtt
      301 tcgtattgct tggtttttta tcgtttttga gtttatgggt ttgtttggtt ttgtttaatg
      361 agcaaactca ttttttaaat caagttagca taggtttgtt aggcgaaaaa tttggatcaa
      421 ttgatgattt gttgctgaca ttaattggaa ctgtaatagt gctgttctat ttttttgctg
      481 gactattaat ttcaacattt gtaacaggtg aaagagaatt gtttagtaca tttataacac
      541 agagttgggt taattttatc agttttggaa ttgtgttttt tgtgttggct ctattgtggt
      601 tgctaaactc accgataaac ccttatattt cgcagctatt tttgtacatt ttactagctt
      661 tatttttagt tagaatgttg aaaattttaa tcgttgcact cttaaatggt gtgtcgtggt
      721 attatttaat tttgtatctt tgcacgctcg aagttctacc tattttagca gtttttgctt
      781 taatggcgtg acatgcgaga agattgttaa cttacaaatt aatttatttt ggctgtaaag
      841 actattttag tatcccaacc aagaccagag actgaaaagt ctccgtattt tgatttggca
      901 gaaaagtaca aattgaaaat tgattttcgt cctttcatcc aagttgaagg gattgaagct
      961 caagaattca gaactcaaaa agttaatatt gctgaacata cagcagtgat ttttacatca
     1021 aaaattgcag ttgaccattt ttttagaatt gctaaagaag tgcgttttga tgttccagat
     1081 accatgaaat atttctgtat ttctgaagca attgcttatt atttgcaaaa atatgtgact
     1141 tatagaaaac gtaaaatttt ctttggtcgc caaacaatag acgatttagt ggtgttaatg
     1201 aaaaagcaca aaacagaaaa gtttttgttg ccttgtacgg atgtgttgca agataaaatt
     1261 cctgaaacca ttaaagagca taaaatcaat ttctcaaaag ctgttttata cagaacagtt
     1321 gctgctgatt tatcagattt ggaagatgtg tactacgaca tgttggtgtt ttttagcccg
     1381 gctggagttg aatcactttt gaaaaatttc ccagatttta aacaaaacga taccataatt
     1441 gctgcttttg gtccaaccac tgcacaatca ttagtgaaaa ataatttgcg tttagacgtg
     1501 catgcgcctc aacctgatgc accttctatg cctgctgcta tcgaattgtt cattaagaac
     1561 gatttgaaaa agaaataata attattttaa cttaaaaagc gttaaatgga ggtcaacttt
     1621 catttaacgc ttttttattt tttacttttg ttggtatgaa aagtattgta ctcattgtta
     1681 tcgttgccat aattggaatt accttgtttt atgtgaataa tcaacctaaa gaagctcaat
     1741 tgcctattta caatccggta gatatcaatc cagaggtggt tgactcttcg ctacaacgtg
     1801 ttggaattgg gcataccatc ggtgatttta cttttaccaa tcaaaatggg caaaccatca
     1861 cgcgcaaaga tgtggatgga aaggtgtatg tggttgaata cttttttaca acgtgtgggt
     1921 ctatttgtcc aatcatgaat aagcaaatgc aacgtgtaca aaaaaaatat gcacaacatg
     1981 atgattttaa aattttaagt tttaccgttg acccgaaaaa tgataccgta gcacaactga
     2041 aacgctatgc agtgcaacat catgccaacg ataaacaatg gtggtttttg acaggagata
     2101 aagaacaatt gtatgcccta gcacgcaaat cgttttttgt gctcaaacct gccgaagctg
     2161 aaaacgtggg agatgccggc agtgatttta ttcacaccaa caattttgtc ttaattgaca
     2221 aaaaacaacg cattcgagga tattacgatg gcaccaatcc tcaaagcgtt gattcactca
     2281 ttaccgatat cgaaaaattg tttgatagta acaattaacc ttcttgttct tctttaaacc
     2341 agctagaata ttttacatag ctgttggcaa tgcgttttat ttcgccttca aacaattctg
     2401 gagaaagtgt ttttactttt ttggcaggaa cgcccgcata aatactccaa ctttcaacac
     2461 gggtgttttc caataataca gctccagccg caatgatgca attggcctta atgtaacaat
     2521 tgtccataac aatagaaccc ataccaatca atacgttgtc ttccactgtg caaccgtgca
     2581 ccaaggcgtt gtgaccaata gaaacgttgt tgcccaatac ggtttttgtt ttttcataag
     2641 tgcaatgaat tacagcacca tcttgcacgt taacacgatt tcccattttt atagaattta
     2701 catcgccacg cacaactgca ttgaaccaaa ccgagcattc atcgcccatt actacatctc
     2761 caactatggt tgcattttct gcaaaccaac aatccttgcc aaattttggc gaagttcctc
     2821 tacatgcttt aattaatgcc atatttttat ttcgttgatt gaattgcgtt taaaataatt
     2881 gctcccgctt ttcttacctc ttcttcggta atggttaagg gtggtgataa gcggaaactg
     2941 taggggtgag ataaaaacca aaaactaatc aacccgtctt ccaagcattt tttgacaact
     3001 ttctccacgc gctcaaacga ttccatgtca aaggcaaaca tcatcccaat tctgcgcatg
     3061 gctttaattt catccgattg tcccacaatg gattgcaaca aagcacccaa gcgttcaacc
     3121 tcgttgaaat caatttcttt ttcaaaaatt tctaaacaag ccaatccagc tgccgctatc
     3181 acagggtttc ctccaaaggt ggtgatgtgt cccaaaattg gatcgtagga aaattcccaa
     3241 agatttttgc gagatgaaac caaaacgcca attggcaaac ctccacccaa tgcttttcca
     3301 agagttaata cgtcgggaac aacgccaaaa tgctcaaaag caaatagttt tcctgtgcgc
     3361 cccattccgc attgaatttc atcaaaaatt agcaatgcac caacttctgt acaacgcttg
     3421 cgcaatgcca ttaaaaattc tttggttgga acacgtacac cagcatcacc ttgcactgtt
     3481 tcgatgataa cacctgctgt tttttcggta atttgctcca attgttcaat gttgttgtgt
     3541 tgcaagaaac gaacatcggg caaaagtgga cgaaacggct cttttttagt ttcatttccc
     3601 gaaacactca tagaaccgtg ggtacttccg tgataagaac ctctaaaagc aaccaattct
     3661 gttcttcggg taacgcgttt tgctaatttc aaagcagctt cgttggcttc tgtgccagaa
     3721 tttacagtat aggcacagtt caattgttct ggaagtaaac caaccagttt tttacaaaaa
     3781 tccaagtggg catcttgaat gaattcgcca tacaccatca cgtgcatgta tttttcggct
     3841 tgttctttta cggctttaat cacatgtggg tggctgtgtc caatgttgtt gactgagaca
     3901 cccgaaatca tgtccatgta ttttttaccg tttttatccg taatgtaaat gccctcagca
     3961 cgttcaacgt caattaaata cggcgtttga ttggtttgcc ctgtatagtt taaaaaatcg
     4021 tctgttctac tcataattgt attcactgca ctaaactttt gtgcaattgt ccaaaattag
     4081 ctttttttga ggtaattttg tgtgcaagaa gataaaaaat caaactaaaa aatgagcgat
     4141 ttgaacatag tggaattgta taaaatgagc aatgagtttg gacgaaccat tgactttgaa
     4201 tacgattttt tacccaatgg cgaagtaacc aacaaaataa taattgataa aaaacacttg
     4261 gcaactcctg taacggcgca tggtggtgta attgccgctt tggcagattc aacacttgga
     4321 attgctgctt tggcagaatc gcaaaaagat gggaatttag tttctactgt tgaatttaaa
     4381 attaactttt tgaaacctgc ttgtttgggc gatgtaattt ctggtttctc gcgtgtgcgt
     4441 aaggtaggga agaaaataat ttttgttgag gcagattttt tgaaccaaaa cgaagagtta
     4501 gttgctactg ctagcggaac gtttaaccaa tatccttttt ctaaagtttt taacctctaa
     4561 acgatgcttg attttcctaa ggattttgta aattatgttg agaatgacgc acatttgggt
     4621 gcagatttgc ttactgcatt gaattctgaa gtacctattt ctattcgaaa aaatccaagg
     4681 aaaacaaaat gtagttttaa agcggaagca ctagttttgt ggtacgagga tgtctatttt
     4741 ttaaaagaac gcccggtttt tacattaaat ccgctgtttc atgctggaac tttttatcca
     4801 caagaagcag gttcgatgtt attggatttt attatccgtt cacttgattt acctgaacaa
     4861 ccaaaatttt tggatttgtg tgcagcaccg ggaggtaaaa gcacgttaat tgcatctttt
     4921 ttagatgata aaggcttgtt ggttgccaat gaggttatca atcaacgggc aaaaatattg
     4981 aaggaaaacg tttctaaatg gggttatccc aatgttgttg ttactaacaa tgatccttcg
     5041 gattttgagc gcactcccaa cttttttgat gtggtagtgg tggatgctcc atgttcgggg
     5101 gagggaatgt ttcgcaagga tgtgcaagca cgaaatgaat ggtcgttgga taacgtaaat
     5161 ttgtgtcaaa gtcgccaaaa acgcattgtt gccgatgtgt gggatgcatt gaacgaaaac
     5221 ggttatttga tttattctac ctgcactttt aatccgcacg aaaacgaaga aaacattcgt
     5281 tggattaccg aaaattttga cgctgaattg gtgactattc cagctccgtt tgattttgtt
     5341 cagggtagag gaggagtagg aatttacggt ataccaggtc gctctcaaac agaagggttt
     5401 tttattgctg ttttgcagaa aaaatcgagt gagggcagaa caaccaaatt gggaaaatca
     5461 caaaacaaag ggctaaccaa ggtcaaagat attggagaac tatccaagtg ggtaaaaact
     5521 acggcttcta ctttttacga gtggaatgct accattttag ccatcccatc tgcatttgaa
     5581 aaagaatatt tgttgttaca aagcaattta cacctcatta aatggggcgt tgaagtgggc
     5641 gaaatagcac gtaaaggatt gattcctagc cacgatttat tgctttgtca cagcttgcga
     5701 ttgaactatc catcgcacga agtgagtgaa caagaagcat tatttttctt gcacggcgat
     5761 acattttcca ttaacggatt gacgcactta ggaatggtgc aattaaccta tcaaaatgag
     5821 cccattggtt ggataaaaaa catcggcaac cgcttcaata atttatatcc gaaagaatac
     5881 cgaatacgga tgaatgtgca gtaaaaatca gtttatttca gcacgtttac cgttccagtc
     5941 cacttataat gattgtcgtt gattctatct ttggcaataa ttacccaagt ataagtgcct
     6001 tcttgcacca cttgaccttt gtaggttcca tcccaagcaa tacttgcatc attggattgc
     6061 caaacagtct ctccccaacg gttataaaca gttagagaaa aattatagag gtcgatacct
     6121 tcggtataaa cacgccaatt ttggttaaac tcatcaccat caggtgtaaa cgtgttaggg
     6181 acgtacaata aaacatcagg caataccacc aattctttta caaccgtatc ttggcaacca
     6241 aattcggtag ttgcaaccaa cattattgga tacgttcctt caactccttc tgggtatttt
     6301 accgatggat tttctaacgt agaaacactt ggagatgctc cttcaaaaaa ccagttgtag
     6361 gcagttgctc ccgaagaaga attgttcatg gtaacgtttg tagaaaaaac tttcaccgga
     6421 ttagggttcc aaacaaaatc ggctgttgga agcggatgaa cggttaagaa atttgtaaac
     6481 gttttcgaat caatacagcc ttgaggcgat acgataagta cttctaccgt ataattgcca
     6541 gcaggcattg gttctgtaat tacattggtt tggtttaaaa acgattgacc atccgaaata
     6601 aaccagttaa cagtttgaat taatgaagga tcggtattgc ataccaattc aaattgagca
     6661 ggctcacaaa ttgatggtga tggcgagctg ataattggtg ttggcaatgg atgtacggta
     6721 accgttgtgt ttaagaccaa tggtgttgat tcgcaagcat ctgttacaat tgctgtgtat
     6781 tgtaatgttt gagctggact cacttgcaaa gaagttccaa tttgattggt attccatgta
     6841 acggtgtatg gatctagtcc gcctgcaatg tttgttactt gcaaagttgt tgtttcgccc
     6901 ggacaaatag ctgcattggt agtgataaca gcactaattg gatttcgcaa ggaaacaacg
     6961 atggtttgag tatcagaaag acatcctgca gcactttcgg cataaaccgt gtaatttgtc
     7021 aatgcagttg gttgaactaa ttgtgtagct gccacatcgg ttgtgtgtcc ccaatggtag
     7081 attgcattcg tttgaccaat agaagcagtt aatgttgctg ttccattttc acaaatcaac
     7141 gtgtcgttgg caactgttaa ttgcaaagca tctggctcta ataatataac tgtattggat
     7201 gttctacaac cgtattgatt ttctgaccat accgtgtaag ttccagcagc taatccagtt
     7261 tttgttgatg atgtttccca agttgctcca ttgtcaaacg aataagaaac agcatcgtta
     7321 ttgatgattt gaatgatacc tgtttgattt ccaaagcatg ttaaatcagt tgtttgtacc
     7381 gtttgtgtgg cttgcgaaac agtaattaaa actgtatcgt tcattgcacc acatccttgg
     7441 ttgctttctg ttaaaataaa tggatatact cctggttggt ttacaacaac ttttgcattg
     7501 agtgttgtgg tatttggaat gtatgtaacc gttggagtag gcgtgattgc ttgtgtatta
     7561 tgcgacc
//
LOCUS       DAOQVQ010000063         7547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10760_length_7547_cov_3.729044, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000063 DAOQVQ010000000
VERSION     DAOQVQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10760_length_7547_cov_3.729044"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKN22_03215"
     CDS             <1..581
                     /locus_tag="PKN22_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNS41745.1"
                     /translation="KNESNNYLNEKATLYFNDSTSVFLIQPFDDVKDENKTFLTTTKM
                     DFSNQTISTSINILGSQFYVRDTIPQRVWKFTGKNRDIAGVKCKQATTKLADGTKIYA
                     WFDTSVLPAVGPESYTNLPGAILGLAYEDGSITYFASTISTSFPEEIATAFPNLNPRK
                     VYSREDFIKEFSSKYDKNDRLYQLIQDLIHFF"
     gene            complement(689..2230)
                     /locus_tag="PKN22_03220"
     CDS             complement(689..2230)
                     /locus_tag="PKN22_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS41746.1"
                     /translation="MKKQILTSIMLACPLFFFAQKNEQMAQKIMDEVYKNSQIEQLSH
                     ELMDDIGPRLVGTPQMKKAHDWAVAKYTSWGIPAENQKYGEWKAWERGITHIDMLSPR
                     VQTLMGTQLAWSPGTSSKGVTAEVIVIPTVSDSIAFQKWLPSVKGKIVMIGMLQPTGR
                     PDHDWKEWATTASFDKMKTEREAQKQAWEANLKKTGYTKRTLPEALEKNGAVAIVDSY
                     WSNGFGAQKVFGAKTKKVPTVDMHLEDYSMLYRMVEHGDKPIIKIVAESKDLGIAPTF
                     NTIAQIKGSEFPNEYVLLSAHFDSWDGGTGATDNGTGTVLMMEVMRVLKKLYPNPKRT
                     IIVGHWGSEEQGLNGSRAFVVDHPEIVQNIQVAFNQDNGTGRIQSINGSGFINAYDYL
                     SRWLKAVPDTIQKVDTYFPGEPGRGGTDYASFIAAGVPSFNLNSLSWSYGNYTWHTNL
                     DTYDKIVFDDMKNNVVLTAILAYMASEDNEKASRERMLLPVDPKTGERQTWPEIKQPE
                     RKGGF"
     gene            complement(2320..3075)
                     /locus_tag="PKN22_03225"
     CDS             complement(2320..3075)
                     /locus_tag="PKN22_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41747.1"
                     /translation="MKNVKTLLLAALTVAVFNANAQIVYRDITPDGLPTNGDFDFNND
                     GTAEFSTDGSMFDYSATNAAGTNMWMDGTAAAGWDEVKPLTVNTVIGASGNFIGYGDA
                     SMDAWGAGTIFPLNVDSYIGVKIAYGTSIHYGWIRVMWNGTDFIYKDYAYNATANATI
                     KAGQISEASVATVEDNQFSIFPNPAKDVISIQAKNTTATTAIITDLAGKVIKQFEATA
                     LNQLNVSDVKSGVYLLNIYAAEKLVATQKIVIE"
     gene            complement(3219..5399)
                     /locus_tag="PKN22_03230"
     CDS             complement(3219..5399)
                     /locus_tag="PKN22_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNS41748.1"
                     /translation="MVRSFWRWSHLILAIVASVVLLAASISGAFLAVEPVQNNNRTTF
                     DNSWNNTTIQHTIEKLKSHYSEITSIEKDKNGFFVADVFTADGEQKIVQFNPTTGKTS
                     GKISEQSAFYSWMTTFHRSLFLDTTGRFLEGITAVLLFFITISGTILVFQRTKSFKAF
                     YDKVEKNLNVQFYHIVFGRLAFIPIIVIALTGGYLFLKRFEVVKTIEPQRLEVDFEHP
                     KEKLGTYSFAHHKIGDIQRIEFPLFEDPMEYYTIFTKEKTLAVDQFTYKILDQENIPT
                     ATQLATLSLDWHTGRKNSVWSIILGLCSISILYFIYSGFKITLLKMKGNFKNKYALGE
                     SEILLLYGSETGNTRNFAKLIYEQLLHEGKKVHLAAMNNYQPATQIQHLIILTSTYGT
                     GDAPAGATKFLQKWKEQPLQQAFSYSIVGFGSLAYPDFCNYALQVEKELAQYPQAKAI
                     LPLVKIHNQSYHSLKTWALDWQKASSIALTLPVNYAVKQLPTHAFTVVKKEHVTVDGN
                     TTFNLLLKTQKKIKFHSGDLLAVYPPNDPFKRNYSIGKLNDSELIISVRLDSKGVCSN
                     YLYSLQENDVLNAAVESNPSFHFNTSKPAIFIGNGTGMGPFLGMLHENTKHQKVALYW
                     GGRTQQSYALYEKYVQAANVENVTTNIALSREQNPKVYVGDLIKRDGKILVEQLKNGS
                     IIYICGTLAMQQSVLEELDGAARQFAQQPLNKFQKNGQLKMDCY"
     gene            complement(5406..5903)
                     /locus_tag="PKN22_03235"
     CDS             complement(5406..5903)
                     /locus_tag="PKN22_03235"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HNS41749.1"
                     /translation="MKRFLNYSKISVFILFVSIGLSFISSEGDIKYKCLIQMVSYKGE
                     KAMVVVSLIKPDGNYDRTLSVLGKDKKWYDEMTKWWDFHSAAKEKIDGITGASIAGSQ
                     RRVVLIKVPSYALNKKYKIRVESGVEDQKYVSNDVEFELNTGNINKNINGKEYVSVVR
                     LIPSN"
     gene            complement(6002..6571)
                     /locus_tag="PKN22_03240"
     CDS             complement(6002..6571)
                     /locus_tag="PKN22_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41750.1"
                     /translation="MATTIKTIQVVVLTYLLFGVFNLFQHHYFLVPVTYSELFVFVMI
                     ASAFVENRKSIDRFHWLLFGYSLFSIAAHPFFWEIFLNPTQQQQLYNTLLFDVVRIIQ
                     WLLLSTFFVLLSYDRDKNVLKLEWLVPAAMTLGCLFNPPLWYVSLTFIISGLSAVYSI
                     KRRLLVTDYIMPVLIGIGALQLINFFYCI"
     gene            complement(6571..7347)
                     /gene="map"
                     /locus_tag="PKN22_03245"
     CDS             complement(6571..7347)
                     /gene="map"
                     /locus_tag="PKN22_03245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNS41751.1"
                     /translation="MIIYKTSDEIAIMREAAQIVSRTLGKVAEMIRPGITGLELDQMA
                     EEYIKSQNAIPGFKGLYGCPSTLLVSINEQVVHGLPTDIKIQEGDIVSVDCGALYKGY
                     YGDHAYTFAIGEVSDEKKQLLKVTEECLYLGIAQAKVGNRIDDIGWAIQTHAEKHGYG
                     VVRDLVGHGLGKKMHEDPQVPNYGRRGHGKKIQNGLTIAIEPMINVGTEKVVVLEDEW
                     TIVTADGKPSAHFEHDIAVVDGEPVILSTFDYIHEALSKK"
BASE COUNT         2270 a         1454 c         1360 g         2463 t
ORIGIN      
        1 aaaaaaatga aagcaacaac tatttgaacg aaaaagcaac gctttatttc aacgattcga
       61 caagtgtttt tttaattcaa ccttttgatg atgttaaaga tgaaaataaa acttttttaa
      121 caacaacaaa aatggatttt agcaatcaaa ccatttcaac gtccattaat attttgggaa
      181 gtcaatttta tgttcgtgat acgattcctc aacgcgtatg gaaatttact ggtaaaaata
      241 gagatatagc aggtgttaaa tgtaaacaag caacaaccaa actagctgat ggcactaaaa
      301 tatatgcctg gtttgataca tcggtgcttc ctgctgttgg tccagaaagt tatacaaatc
      361 ttcctggagc tatacttgga ttggcttatg aagatggttc aattacctat tttgcatcaa
      421 caatttcaac ctcttttcca gaagaaattg ccacagcatt tcccaatctg aatcctagaa
      481 aagtctattc aagagaggat tttataaaag aattttcttc gaaatacgat aaaaatgatc
      541 gcttgtatca gttaatccag gatttaattc attttttctg ataacatttt atctcctaga
      601 tgtaaaccaa actttgttgg attttttgat tacaaaaaaa gcggtattgt ctatcacagt
      661 accgcttttt ttatgctttt aaaagaatct aaaaccctcc ttttctctca ggttgtttga
      721 tttctggcca cgtttgacgt tctcctgttt tggggtcaac aggcaataac atgcgctcac
      781 gtgaagcttt ttcgttgtct tctgatgcca tgtaggctaa aattgcagtg agaacaacat
      841 tgtttttcat atcatcaaac acaattttgt catacgtatc caaattggtg tgccatgtat
      901 agttgccata actccaactc aatgaattga ggttaaatga aggaactcca gctgcaataa
      961 acgaagcata atctgttcca cctcttccag gctcgccagg gaaataagta tctacttttt
     1021 gaatggtgtc gggcaccgct tttaaccaac gcgacaaata gtcgtaggca ttgataaatc
     1081 cagacccgtt aatactttga atgcgccctg ttccattgtc ttgattaaat gccacttgga
     1141 tgttttgcac aatttctgga tggtcaacca caaaagcacg tgagccgttc aatccttgct
     1201 cttcgcttcc ccagtgtcca acaataatgg tgcgtttagg gttgggatac aattttttca
     1261 atacacgcat cacttccatc atcaataccg tacctgttcc gttgtctgtt gcacctgttc
     1321 caccatccca cgagtcaaaa tgagcagaca gcaacacata ttcgtttgga aattcactgc
     1381 cttttatttg tgcaatggtg ttaaatgttg gcgcaattcc caaatctttg gattctgcaa
     1441 ctattttgat gataggttta tcaccgtgtt caaccatacg ataaagcatt gaataatcct
     1501 ccaaatgcat gtcaacagtt ggaacttttt tggttttagc tccaaaaact ttttgcgcac
     1561 caaatccgtt cgaccaatag gaatcaacaa tggcaacagc accatttttt tcaagcgctt
     1621 ctggcaacgt gcgtttggta tagcctgttt tcttcaaatt ggcttcccac gcttgctttt
     1681 gagcttcgcg ttcggttttc attttatcaa acgatgctgt agttgcccat tctttccaat
     1741 cgtggtcggg gcgaccagta ggttgcaaca ttccaatcat cacaatttta cctttaacac
     1801 ttggcaacca tttttgaaaa gcaatagaat ctgaaaccgt tggaatgaca ataacttcgg
     1861 ctgttacacc tttggatgat gtgcccggac tccacgccaa ttgggtgccc atcaatgttt
     1921 gaacgcgcgg cgataacatg tcaatgtgcg taattccacg ttcccacgct ttccattcac
     1981 cgtatttttg attttcggct ggaattcccc aactggtata ttttgccaca gcccaatcgt
     2041 gtgctttttt catttgtggg gttcctacca aacgcggacc aatgtcgtcc attaattcgt
     2101 gtgaaagttg ctcaatttgt gagtttttat aaacttcatc cataattttc tgcgccattt
     2161 gttcgttctt ctgagcaaag aaaaacaacg gacaagcaag cataatggag gtgagtatct
     2221 gttttttcat gtttgtaaat tttatacttc caacaaatat aaaaaaaatg ggatactttt
     2281 ttaacaaagc tcccatttcg aaataaggtt atagagttat tattcgataa cgattttttg
     2341 agtagctact aatttctcag cagcgtaaat gttcaacaaa taaacacccg atttcacgtc
     2401 gcttacgttt aattggttca atgctgttgc ttcaaattgt ttgatgactt tgcctgccaa
     2461 gtcggtaata attgcagttg ttgcagttgt gttttttgct tgaatgctga ttacatcttt
     2521 tgcaggattt gggaaaatag aaaactgatt gtcttctact gttgcaacac tagcttctga
     2581 aatttgtccg gctttaatcg ttgcatttgc agttgcgtta taagcataat ctttgtaaat
     2641 gaaatcagtt ccattccaca ttacacgaat ccatccgtag tgaatagatg tgccataagc
     2701 aattttcacc ccaatgtagc tatcaacatt taatgggaaa attgtaccag caccccaagc
     2761 atccatagaa gcatcaccgt agccaataaa attaccactt gcaccaataa ctgtgttaac
     2821 ggttaagggt ttcacttcat cccatccagc agcagccgtt ccgtccatcc acatgttggt
     2881 tccagctgca tttgttgcag aatagtcaaa catgcttcca tctgtcgaaa attcagcggt
     2941 tccgtcattg ttaaagtcga aatcaccatt tgttggcaaa ccatctggag tgatgtctct
     3001 gtaaacaatt tgtgcgtttg cattaaatac agccactgtt aaagcagcca aaaggagagt
     3061 ttttacattt ttcatattgt tgaatatttt taaattaagt atccaaaaga cgaggcaaaa
     3121 acgatttagt tgctttaaat tttaaaaaat aataagaaat tgttaaaaat acaacatttt
     3181 taaggccata agtaaatgaa aatcaaaatg aaaaatggtt aataacaatc catttttagt
     3241 tgcccatttt tttggaattt gttcaaaggt tgttgtgcaa attgacgtgc tgcaccatcg
     3301 agttcttcca aaacactttg ttgcatggca agcgttccac aaatgtaaat gatgctgcca
     3361 ttttttagtt gttcaaccaa aattttgcca tcccgtttga ttaaatcgcc aacatacact
     3421 ttaggattct gttcacgcga taaagcaata ttggtagtta cattttcaac atttgcagct
     3481 tgcacatatt tttcatacaa agcatacgat tgctgtgtgc gtccgcccca ataaagcgcc
     3541 actttttggt gttttgtgtt ttcgtgtaac atgcctaaaa atggtcccat tccagttccg
     3601 tttccaataa aaatagccgg ttttgaagtg ttgaaatgaa acgatggatt gctttctaca
     3661 gctgcgttga gcacatcgtt ttcttggagt gaataaaggt aattagaaca tacacctttg
     3721 ctatccaaac gcacactgat gattaattct gaatcattta attttcctat ggagtagttg
     3781 cgtttgaaag gatcgtttgg tggatagacc gccaataaat ctcctgaatg aaatttgatt
     3841 tttttctgtg tttttaatag gaggttaaat gtggtattgc catcaactgt tacgtgttct
     3901 tttttcacaa ccgtaaaggc gtgagtggga agttgtttga ccgcataatt gacaggaagt
     3961 gtcaatgcaa ttgaacttgc tttttgccaa tccagtgccc aagtttttag tgaatgatac
     4021 gattggttgt gaatttttac caatggtagg atggctttgg cttgaggata ttgcgccaat
     4081 tctttttcaa cctgtagtgc ataattgcaa aaatcagggt aggccaacga accaaaacca
     4141 acaatggagt aggaaaatgc ttgttggagc ggttgttctt tccatttttg aagaaatttt
     4201 gttgcacctg ctggagcatc tcctgttcca taagtagaag tgaggatgat gaggtgctga
     4261 atttgcgtag caggttgata gttattcatc gctgcaaggt gcaccttttt accttcgtgc
     4321 aaaagctgtt cgtatattaa ttttgcaaaa ttgcgagtat ttcctgtttc actgccatac
     4381 aacaataaaa tttcgctttc acctaaggca tatttgtttt tgaaattccc cttcattttt
     4441 agcaaggtaa ttttgaatcc agaatagatg aaatacaaaa tgctgatgga gcaaagtcct
     4501 aaaatgattg accaaacgct attttttctt cctgtgtgcc aatccaaact taaggttgct
     4561 aattgtgttg ctgtggggat gttttcttgg tctaaaattt tataggtaaa ctggtcaacc
     4621 gccaaggttt tttctttggt aaaaatggtg taatattcca ttgggtcttc aaacaatgga
     4681 aactcgatgc gttgaatgtc gccaattttg tggtgtgcaa aagaataagt gcccaatttt
     4741 tctttgggat gttcaaaatc tacctcaagg cgttgtggtt caatagtttt cacaacctca
     4801 aaacgtttta aaaacaaata accgcccgta agcgcaatga caatgatggg aataaatgcc
     4861 aatcgaccaa aaacaatgtg gtaaaactgt acgttgaggt ttttttctac tttatcgtaa
     4921 aatgctttga acgatttggt gcgttgaaat acaagtatag tcccagaaat ggtaataaaa
     4981 aacaacaaaa cggctgttat tccttctaaa aaacgacctg ttgtgtctaa aaacaacgaa
     5041 cggtgaaaag ttgtcatcca actataaaaa gctgattgtt cgctaatttt ccctgaagtt
     5101 tttcccgttg ttgggttgaa ttgcacaatt ttttgttcac catcggctgt aaaaacatcg
     5161 gcaacaaaaa agccattttt atctttttcg atgctggtaa tttccgaata gtgggatttt
     5221 aatttttcaa tggtgtgttg aattgttgtg ttgttccacg aattatcaaa agttgtacgg
     5281 ttgttgtttt gaacaggttc tacggctaaa aaagcaccac taatggatgc tgcaagtaaa
     5341 acaacagatg caacaatggc taaaattagg tgactccatc tccaaaacga acgtaccata
     5401 aacaattaat tacttggaat taaacgaaca acactcacgt attctttccc attgatgttt
     5461 ttgttgatat tgcctgtatt gagttcaaat tcaacgtcat tggatacgta tttttggtct
     5521 tctacccctg attctacacg gattttgtat tttttattga gggcatagga aggaactttt
     5581 atcaaaacaa ctctgcgctg acttcctgca atggatgcac cggtaatacc atcaattttt
     5641 tctttagctg ccgaatgaaa atcccaccat ttggtcattt catcgtacca ttttttatct
     5701 ttcccaagta cgctaagggt tctgtcgtaa tttccatccg gttttataag tgaaacaacc
     5761 accattgctt tttcgccttt gtaactcacc atttgaatta agcatttgta tttgatatcg
     5821 ccttcgctag agataaacga caatccaata cttacaaata aaatgaaaac actgattttg
     5881 ctataattta aaaaacgttt catgcactta tgttttgatt atttgagtaa acttttcttg
     5941 taaaagttac aaaaataatg aatttatgga tgtttttatc caattattgc gccaaaaaat
     6001 actaaataca ataaaagaaa ttaatcaatt gcaaagctcc aattccaatt aaaacaggca
     6061 tgatgtaatc ggttactaac aagcgtcgtt ttatggaata tacagcactc aaccccgaaa
     6121 tgatgaaagt aagggaaaca taccaaaggg gaggattgaa caaacaacca agtgtcatgg
     6181 cagcgggtac caaccattct aattttagca cgtttttatc acgatcatag ctcagcaaaa
     6241 caaaaaaggt agaaagcaat aaccattgga taatgcgcac aacatcaaac agcaaggtgt
     6301 tgtacaattg ttgttgttgg gttggattga ggaaaatttc ccaaaaaaat ggatgtgcag
     6361 cgatagaaaa taaagaataa ccaaagagca accaatgaaa acggtctatt gattttctgt
     6421 tttctacaaa agcactagca atcattacaa aaacaaacaa ttctgaatag gttacaggca
     6481 ccaaaaaata atggtgttga aacaaattaa aaacaccaaa taataagtag gttaaaacta
     6541 caacttggat ggttttaatg gtggtagcca ttatttttta gaaagtgctt cgtgaatgta
     6601 atcaaatgta gaaaggatta ctggttcgcc atccactact gctatgtcat gttcaaaatg
     6661 ggcacttggt tttccatcag ccgttacaat ggtccattca tcttctagaa ccaccacttt
     6721 ttctgtccca acattaatca ttggttcaat ggcaatggtg agtccgtttt gaattttctt
     6781 accatgtccg cgacgtccgt agtttggaac ttgaggatct tcgtgcattt ttttacctaa
     6841 tccgtgtcct accaaatcac gtacaacgcc atagccgtgt ttttcagcat gtgtttgaat
     6901 ggcccagcca atatcgtcga tgcggttacc tacttttgct tgtgcaatac cgaggtacaa
     6961 acattcttcg gttactttta acaattgttt tttctcatcc gatacttcgc caattgcaaa
     7021 tgtataggca tgatcgccat aatatccttt gtaaagcgca ccacaatcta ccgaaacgat
     7081 gtcgccttct tgaattttaa tatcggttgg aagtccgtgc accacttgtt cattgataga
     7141 caccaacaaa gtactagggc aaccatacaa tcctttaaaa ccagggattg cattttgaga
     7201 tttgatgtat tcttcggcca tttgatccaa ttccaaacct gtaattccag ggcgtatcat
     7261 ttcggctact tttcctaatg ttctactcac aatttgagca gcttcgcgca tgattgcgat
     7321 ttcatcggat gttttgtaaa taatcatatc tctacgtaaa aatttcattt gtgggtgcaa
     7381 aaatacaaaa aagacggcac aattgattca attctattat ttcacttctt gcattaattt
     7441 gcgcaccaat ggcgaaatag caatcaacac aatgcctgca atcaaggcat aaatggccaa
     7501 ttgcaaatat ccatcggtgt aggtcattaa tttttgagtt ggtgaat
//
LOCUS       DAOQVQ010000064         7466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10899_length_7466_cov_3.268115, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000064 DAOQVQ010000000
VERSION     DAOQVQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10899_length_7466_cov_3.268115"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKN22_03250"
     CDS             <1..316
                     /locus_tag="PKN22_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNS41752.1"
                     /translation="KVRKKIYFVTFLLSAITALEVIVGIFASRANTFGAAWETIKWMY
                     VALTIVKAYYIVMVFMHLGQEKRDLKLIVLVSYIILIIYLVFVLMDEGAAVGHDWSFY
                     GA"
     gene            327..998
                     /locus_tag="PKN22_03255"
     CDS             327..998
                     /locus_tag="PKN22_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41753.1"
                     /translation="MTNKKIAGRVRGSYIVLLLFAPALLLFITSRGCKMKFKTLEDFG
                     KINNYSFVDANGKQYTQNDFKDNSVIFINLQESCLDTCSINLFQFDKIIYQDIRGKKT
                     LKKVKIVSFINDMNGNPVKDLSEAKKLIEDNVYKYDPERWIIASGDAKNVFDMENNGQ
                     SLLQTGDEYFGGNAFQELMLLADNNNHLRMVLRGNVEGMYRRMKECIALLQKEYSLKN
                     EVANK"
     gene            979..1641
                     /locus_tag="PKN22_03260"
     CDS             979..1641
                     /locus_tag="PKN22_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNS41754.1"
                     /translation="MKLPISKIIPLLFLTLACTEKKEEYRLPIIGEKDVQYTTVNGKE
                     VADTIYHTVPDFEYLNQDSVMVSSKELKTKIWVADFFFTSCPSICPSMTTNMKRLSEN
                     TKDLKSQVQFLSFTIDPYTDKPSRLRQYIKERGLDASNWQFFVGDEEATHLLAKEFFN
                     AAEKNDSIPGGFGHTPYFSIIDTQGHIRGIYDGTNARAVDSLENDLRTLIKTEYNGEK
                     RK"
     gene            1622..2185
                     /locus_tag="PKN22_03265"
     CDS             1622..2185
                     /locus_tag="PKN22_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNS41755.1"
                     /translation="MEKKENKSVRRIIIVVSILIPFIVAALFSVKVKGVDLGFLPPIY
                     VSINAVTSVLLVAALMAVKKKKFELHERLMKLSMLCTLLFLIGYIAYHITSDTSVFGD
                     INHDGIRDDVEKATIGSSLYVYLFFLLTHITLTPFITPLVLFAYLFAHEKNNKMHKKI
                     GKIAWPIWFYIAVSGVIVYLMNMPYYL"
     gene            complement(2182..2865)
                     /locus_tag="PKN22_03270"
     CDS             complement(2182..2865)
                     /locus_tag="PKN22_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS41756.1"
                     /translation="MNNSIVLVEDEQSIAEVVLMNLELENYKVTVFSNGRQALENLPL
                     LLNANLVILDVMLPEVNGIDICKEIRKHSSVPILFISAKGTSSDKIEGLKSGGTDYLG
                     KPFDLEELLLRVNILIGRTNAQQHLKIGKNTVDFRTYVVTDEQNQKIAELSKREIELL
                     QLFNAKQGEVISRDEILDKVWGIDQYPTSRTIDNFILSFRKLFEENSKTPKYFHSIRG
                     VGYKFTLVD"
     gene            2962..5397
                     /locus_tag="PKN22_03275"
     CDS             2962..5397
                     /locus_tag="PKN22_03275"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNS41757.1"
                     /translation="MKKITLGIVVALMSFMPFMNHAQSNYPAIEKFSKNIPTLYYQTP
                     DLSAIAQQDIQRDKNGQFYRIGVGIDVNITPQNSGVWSTNENGDKVWQLKIQYPGAEA
                     LNFIFSKFYIYGNTTLNVYNTQGKKIHTAFTSKDVLEHGQQNLDLCQGDNMVLELVEP
                     NGVQPSLIEIEQIGYIYRGAGFPSKANNEKDFGDSESCEVNVNCSPEGNNWQDEKKGV
                     ARILLKVGSQYGWCSGSLVNNTNQDCTPYFLTAQHCAEGVSTSDLNLWKFYFNYEAAG
                     CTNPGSQGTLANSLITGCLKIASSEDVSGNTISKSDFFLCKLGNASNQSTIITTLKGF
                     GAYWNGWDANNTASASGVSIHHPAGDIKKISTYTSTVSSTSYSGVTANTHWQVTWAAT
                     TNGHGVTEGGSSGSPIFTYNGGNSRIVGTLSGGSSFCTATSSPDLYGKVSYHWTSCGT
                     TNAMQLKPWLDPANSGVLVLNGSSDPCASGGTAPTANFSADQTTVPTGTTVNFTDLSS
                     NSPTSWTWAVTPATGWVYTGSTSATSQNPKITFNTAGTYSVQLTAANSYGSDVELKSS
                     YIVVTSSGNTPCTATSTTCDEFIAQVQLGTINNSTACTNYTDYSSLSTNLTKGQQYTI
                     SIIGQIGTNFGSLYSSDQISAWIDYNNDNDFSDAGEQIVLEAVTSSGYSGTYTFTVPT
                     TVTVGTKRLRVRIDYVQSGSISPCGSTQYGEVEDYRVNIVNSNAGIDNIALNNVIMYP
                     NPTANKVTLDLGTTYQTIDVTVTDISGKVVLTSTERNKTALQLTVADLTPGVYQVTVN
                     TEAGKIVKRLIKE"
     gene            5518..>7466
                     /locus_tag="PKN22_03280"
     CDS             5518..>7466
                     /locus_tag="PKN22_03280"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNS41758.1"
                     /translation="MKRKILLFFAVGLSVLISSKAFSQSYLGIQNSNYVGVMGLDNQP
                     ASFVDGRFVVDINLFSVNMGVWNNVKKFDTRDMPGWWVKSFRDETQWMEPDSTFADRY
                     LLDNYKLGSKKKHGAIFNTQIDLLNFAFHITPKIALGFGVKARSVVNIEDFSPELIKL
                     AENGFDYSSLFYKQLNDQNLNASMMAWKEYKINYGQVILDKGEHFLKGGIGIKFLQGI
                     GSGYLHSNNVNYGFNNKDTSFLLQGDFSYGYSQNLEEGLDAKDILTGSASRLGVGFDL
                     GFVYEWRPKHQDYKYDMDGKTNIWRQDLNKYKLRAGVSFLDIGGMRYVKGGKSRDFSV
                     NETNPFDMTVFKDADDIETFDHILDSLLTYDPDWKEKENTGQTYFHNLPTAMSLQFDY
                     QIWKDFKVDVTALFNLIPKKSDSRVRVANQITITPSYDWSWVSIHFPLSYNTFSGFKA
                     GVASRLGPITVGITDFRALFAKGKVRGTDIYVGLRVPILYGKPKDRDNDKVSDKKDEC
                     KDVPGVWKFMGCPDTDGDGIKDAEDECPAEAGIAAFKGCPDTDGDGIPDKNDNCPEEA
                     GLPQFNGCPDKDSDGIMDKNDECPDVPGIPAFNGCPDSDGDGIPDHKDACPNAAGPEI
                     NDGCPDTDGDGVLDYLDNCPTVAGPA"
BASE COUNT         2458 a         1201 c         1406 g         2401 t
ORIGIN      
        1 aaaagtacgt aagaaaattt attttgtaac gtttttactt tctgcaatta ccgctttaga
       61 agttattgta ggtatttttg ctagccgtgc aaacacattt ggtgcagcat gggaaacaat
      121 taaatggatg tatgttgcct taacaatcgt aaaagcttat tacattgtta tggtgttcat
      181 gcacttgggt caagaaaaaa gagatttgaa acttattgtt ttagtttctt atatcatttt
      241 gattatttac ctagtttttg tattaatgga tgaaggagca gctgtaggac acgattggag
      301 tttctacggt gcataaataa ttaattatga ctaataaaaa aattgccgga cgagtaagag
      361 gttcgtatat cgtacttctc ttatttgctc cggctctttt attgtttatc acttcaagag
      421 ggtgtaaaat gaaatttaaa actcttgaag attttggtaa aatcaacaat tattcgtttg
      481 ttgatgccaa tggtaaacaa tatacacaaa acgattttaa agacaattct gtaattttca
      541 ttaatttaca agaatcgtgt ttagatacat gcagtatcaa cttatttcaa tttgacaaaa
      601 ttatttacca agatattcgt ggtaaaaaaa cattgaaaaa agtaaaaatt gtttcattca
      661 ttaacgatat gaatggaaat cctgtcaaag atttatctga agcaaaaaaa ctcattgaag
      721 acaatgttta taaatacgac cctgaaagat ggatcattgc ctctggtgat gctaaaaatg
      781 tgtttgacat ggaaaacaat gggcaatcgt tattgcaaac aggtgatgaa tattttggcg
      841 gcaatgcttt tcaagaatta atgttacttg ccgacaataa caatcacttg cgcatggttc
      901 ttcgcggaaa tgtggaagga atgtatcgta gaatgaaaga atgtattgcc ttacttcaaa
      961 aagaatacag tctaaaaaat gaagttgcca ataagtaaaa taattccact tctttttctt
     1021 acacttgctt gtacagaaaa aaaagaagaa tatcgtttgc ctatcattgg tgaaaaagat
     1081 gttcaatata ctacagtcaa tggtaaagaa gtggcggata ctatttatca cactgtaccc
     1141 gattttgaat acctcaacca agattctgta atggtttcat ccaaagaatt gaaaaccaaa
     1201 atttgggtgg ccgatttctt ttttacctcg tgtccttcca tttgcccatc tatgactacc
     1261 aacatgaagc ggttgagtga aaacacgaaa gatttaaaga gtcaagtgca gtttttaagc
     1321 tttacgattg atccctatac cgataaacca agtcgcttac gacaatacat caaagaacgt
     1381 gggttggatg cttccaattg gcagtttttt gttggagatg aagaagctac gcatttattg
     1441 gctaaagaat tttttaatgc tgctgaaaaa aacgattcca tacctggagg ctttggtcac
     1501 accccctatt tttcaattat tgatactcaa ggacatatca gaggaattta cgatggaaca
     1561 aatgctcgtg cagttgactc cctcgaaaat gatttaagaa cactaattaa aaccgaatat
     1621 aatggagaaa aaagaaaata aaagtgtacg tagaataatc atcgttgttt cgattcttat
     1681 tccatttatt gttgctgcct tatttagtgt aaaagttaaa ggtgtagatt taggtttctt
     1741 gcctccaata tatgtcagta ttaacgccgt aacatccgta ttactggttg ctgcattgat
     1801 ggctgtgaaa aagaaaaagt ttgaattgca tgagcgctta atgaagttaa gcatgttgtg
     1861 caccttgctc tttttaattg gctatattgc ctatcacatc acatccgata cttctgtttt
     1921 tggagatatt aatcacgatg gaattcgtga tgatgttgaa aaagcaacaa ttggaagttc
     1981 tctttacgtt tatttatttt tcttgcttac gcacatcaca ttaacgccat ttattacgcc
     2041 gttggtattg tttgcctatt tgtttgcgca cgagaaaaac aataaaatgc acaaaaaaat
     2101 aggtaaaatt gcttggccaa tttggtttta tattgcagtg tcaggtgtta tagtttactt
     2161 gatgaatatg ccttattatc tttaatcaac aagtgtaaat ttgtaaccaa cacctcgaat
     2221 ggaatggaaa tatttgggtg tttttgagtt ttcttcaaac aattttctga acgataaaat
     2281 aaaattatca atagttctag aggttggata ttgatctatt ccccaaactt tatcaagaat
     2341 ttcatctctt gagattactt ctccttgttt ggcattgaat aattgtaata attctatttc
     2401 gcgttttgac aattcagcaa ttttttgatt ttgttcatct gttacaacat aagttctaaa
     2461 atcaactgtg tttttaccaa ttttcaaatg ttgttgagcg tttgtacggc cgattaatat
     2521 gtttacacgc aatagcaatt cttccaaatc gaatggtttg ccaaggtaat ctgttccacc
     2581 actttttaat ccttcaattt tatccgaact ggtacctttg gctgaaataa acaaaatagg
     2641 aacagatgaa tgttttctaa tctctttgca aatgtcaata ccattaactt cgggcaacat
     2701 aacatctaaa atcaccaaat ttgcatttaa taataatggt agattttcca atgcttgtct
     2761 gccattacta aaaacagtta ccttatagtt ttcaagttcc aaattcatca atacaacttc
     2821 ggcaattgat tgttcgtctt ctacaagtac aatggaattg ttcataaatc aaatctttgt
     2881 tgacaaaaat agaactaaga ttttgattga gaacaaaaaa aaattaaatt tgaaacacta
     2941 taaatcataa aaaactagtc tatgaaaaaa attacgttag gtatagtcgt agctttgatg
     3001 tcgttcatgc cctttatgaa ccacgcacaa agcaattatc cggctattga aaaattctct
     3061 aaaaacatcc ccacgttgta ttatcaaaca cccgatttga gtgctattgc tcaacaagat
     3121 attcaacgtg ataaaaatgg tcaattctat agaattggtg tcggaattga tgtaaacata
     3181 acgcctcaaa acagtggtgt ttggtcaacc aatgaaaatg gagacaaagt ttggcaattg
     3241 aaaatacaat atccaggtgc agaagcatta aactttatct tttctaagtt ctacatttat
     3301 ggcaatacaa cattaaatgt ttacaacaca caaggtaaaa aaattcacac tgcattcact
     3361 tccaaagatg tgctggaaca tgggcaacaa aatttagacc tttgtcaagg cgacaatatg
     3421 gtgttagaac ttgttgagcc taatggagtg caaccttcat tgattgaaat tgagcaaatt
     3481 gggtatatct acagaggtgc tggatttcca tcaaaagcaa ataatgaaaa agattttggt
     3541 gattcagaaa gctgtgaagt caatgtaaat tgctcaccag aaggtaataa ctggcaagac
     3601 gagaaaaaag gagttgcgcg tattttatta aaagttggtt cccaatatgg ttggtgttct
     3661 ggttctttgg taaacaatac caatcaagat tgtacaccct atttcttaac ggcgcagcac
     3721 tgtgctgaag gtgtttcaac ttctgatttg aatttgtgga aattttattt caattatgag
     3781 gctgctggat gtactaatcc tggttcccaa ggtactttgg caaatagctt aattacaggt
     3841 tgtttaaaaa ttgcttcttc agaagacgta agtggaaata ccataagtaa atctgacttt
     3901 ttcttatgca aattaggaaa tgcctctaat caatcaacca ttatcacaac cttgaaaggt
     3961 tttggtgctt attggaatgg ttgggacgca aacaatacag catctgcaag tggagtaagt
     4021 attcaccacc cagcaggtga tattaaaaag atttcaactt atacaagtac tgtttcttct
     4081 acatcgtata gtggtgtaac tgctaataca cactggcaag taacttgggc tgctacaaca
     4141 aacggacatg gtgttacaga aggaggctca tcaggttctc ctatatttac atacaacgga
     4201 ggaaattcta gaatagttgg aacattatct ggtggttctt ctttttgtac agcaacctca
     4261 tctccagatt tatatggtaa agtttcatac cattggacaa gctgtggaac taccaatgca
     4321 atgcaactaa aaccttggtt agaccctgcc aactcaggtg ttttagtatt gaatggttca
     4381 tcagatcctt gtgcttcagg aggaacagct ccaactgcca acttttctgc tgatcaaact
     4441 acagttccaa caggcacaac tgttaatttt actgatttaa gttctaattc tccaacatct
     4501 tggacgtggg cagttacacc agcaactggt tgggtatata caggtagcac aagtgcaaca
     4561 tcacaaaacc ctaaaataac ttttaatact gctggtacct atagtgttca gctaacagct
     4621 gctaatagtt atggttctga tgtggaatta aaatcaagct atattgttgt tacatcttct
     4681 ggaaatacac catgtacagc aacatctaca acatgtgatg aatttattgc tcaagttcaa
     4741 ttaggaacaa tcaataactc aacagcttgt acaaattata cggattattc ctctttatca
     4801 accaatttaa cgaaaggaca acaatatact attagtatta ttgggcaaat tggaactaat
     4861 tttggaagtt tatactccag cgatcaaata tcagcatgga ttgattacaa taacgacaat
     4921 gatttctcgg atgctggtga acaaattgta ttagaagcag ttacatctag tggttattct
     4981 ggaacgtaca cctttactgt tcctacaaca gttacagttg gaacaaaaag acttcgtgta
     5041 agaatagatt atgtacaatc tggttcaatt tctccgtgtg gcagcacaca atatggtgaa
     5101 gtagaagatt accgtgttaa tattgttaac tccaatgctg gaattgataa cattgctttg
     5161 aataatgtaa ttatgtatcc aaatcctact gcaaataaag ttacattgga tttaggtact
     5221 acctaccaaa caattgatgt aacagttaca gatatttcag gtaaagttgt tcttacatca
     5281 actgaaagaa ataaaactgc attgcaatta actgttgcag atttaacacc aggtgtatat
     5341 caagttactg taaatacaga ggctggtaaa atcgtaaaac gtttgattaa agaataattt
     5401 ttaaatttag atttaaggga aagtcgctca atttgggcga ctttttttgt tgaaaaaaca
     5461 cttaaacaat ttggatattt gagttttatt tatattttta ctttaaaata cttatttatg
     5521 aaaaggaaaa ttctactgtt ttttgctgtt ggacttagtg tactcataag ttcaaaagca
     5581 ttctcacaaa gttatcttgg aattcaaaat agtaattatg tgggggttat gggcttggat
     5641 aaccaacctg caagttttgt tgatggtcgc tttgtagttg atattaatct ttttagtgtc
     5701 aacatgggag tttggaacaa cgtcaaaaag tttgatactc gagatatgcc tggttggtgg
     5761 gtaaaatcgt ttagagatga aacccaatgg atggaacccg attcaacttt tgcagatcgt
     5821 tatttgctag acaattacaa attgggtagt aagaaaaaac atggtgctat tttcaatacc
     5881 caaattgatt tgttgaactt tgcatttcat attacaccta aaattgcttt gggttttggt
     5941 gtaaaagcac gttcggttgt taatattgaa gattttagcc ctgagttgat taaacttgct
     6001 gaaaatgggt ttgattactc gtctttgttt tacaagcagc tcaacgacca aaatctaaac
     6061 gcatctatga tggcttggaa agaatacaag atcaattatg ggcaagttat tttagataaa
     6121 ggtgaacact tcttaaaagg tggtattggc atcaagttct tgcaaggaat tggttctgga
     6181 tacttgcatt ctaacaacgt aaattatgga tttaacaaca aagatacatc gtttttattg
     6241 caaggtgatt tttcttacgg atattcccaa aatttagaag aagggttaga tgcaaaagat
     6301 attttgactg gttctgcatc ccgtttgggt gttgggtttg atttaggatt tgtctatgaa
     6361 tggcgaccta aacatcaaga ttataaatat gatatggatg ggaaaaccaa catttggcgt
     6421 caagatttga ataaatataa attacgtgct ggtgtttcat ttttagacat tgggggaatg
     6481 cgttatgtaa aaggcggtaa aagtagagac tttagtgtca atgaaaccaa tccttttgat
     6541 atgactgtat ttaaagatgc agacgacatc gaaacatttg accatatttt ggatagttta
     6601 ctaacatacg accctgattg gaaagaaaaa gaaaatacgg gtcaaaccta tttccacaac
     6661 cttccaacag cgatgagttt gcaatttgat taccaaatat ggaaagactt taaagtagat
     6721 gtaacggcat tgtttaattt aataccaaag aaaagtgata gccgtgtgcg tgtggcaaat
     6781 caaattacaa tcacgccaag ctatgactgg tcgtgggtga gtatccactt ccctctttcc
     6841 tacaatactt tctctggatt taaagcaggt gtggcttctc gtttaggacc aatcacagtt
     6901 ggtattactg actttagagc attgtttgca aaaggaaaag tgcgtggtac agatatttat
     6961 gttgggctga gagtgccaat tctttacggt aaaccaaaag atagagacaa cgataaagta
     7021 tctgacaaaa aagatgagtg taaagacgta cctggagtat ggaaattcat gggttgtcca
     7081 gacacagatg gagatggcat caaagatgca gaggacgaat gtccggcaga agctggtatt
     7141 gcagcattta aaggatgtcc agatactgat ggtgatggta ttcctgataa aaacgacaat
     7201 tgtccagaag aagcaggttt acctcaattc aatggatgtc cggataaaga tagcgacggt
     7261 atcatggata aaaacgatga atgtccagat gtaccaggta tcccagcgtt taatggttgc
     7321 ccagattctg atggagatgg aattccagac cacaaagatg cttgtccgaa tgctgctgga
     7381 ccagaaatca atgatggctg cccagataca gatggtgacg gtgtgttgga ttacttagac
     7441 aattgtccaa cagttgctgg acctgc
//
LOCUS       DAOQVQ010000065         7430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_10977_length_7430_cov_3.486644, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000065 DAOQVQ010000000
VERSION     DAOQVQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10977_length_7430_cov_3.486644"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKN22_03285"
     CDS             <1..656
                     /locus_tag="PKN22_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNS41759.1"
                     /translation="LAHGFKDKADRLGDVNGGLFTYPMLMAADILIYDAEFVPVGKDQ
                     EQHIEFTRDVASRFHAQFKETFVIPEGLVNENTKIIPGTDGQKMSKSRNNFINIFLPD
                     KQLRKQIMSIQTDSKELEEPKDPATCNVFALYKLLATDEQIQNMTELYVNGGYGYGHA
                     KQALFDLIVEKYKNQRERYWYLMEHPEEIIAALKIGEDKARKYAKTVLERVRATVGY"
     gene            798..2504
                     /locus_tag="PKN22_03290"
     CDS             798..2504
                     /locus_tag="PKN22_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS41760.1"
                     /translation="MKSIIYVTLSTFLLLTSCGNKETKTKDITLINGGKTYGGELVFS
                     SSEVVSETFPIASANVNVINISSQIYETLLNIDPTTLEVVPGIASEFSSNEEGNVYTF
                     KIRKGIFFHDDECFGGKGRELTPEDVKFSLDFVCSGNPLNQDDDILVNRIKGAKEFKS
                     KSATSFPKGGVSGIKVKGDAVEITLNKPFIGFEELLTRKNTIIFPKEAFEKYGNDIKT
                     HPVGTGAFKLESIDKSQIRLVRNDNYWQKDEFGNQLPYLSAVVLKFIPNKKDEMLAFR
                     NKNIDLLLDIPAAEIENVLGTLQDAMAGKNVKHKVESSQSLNIEYVGFNTADGVFASK
                     EVREAFLYAVNPSELIEKYIGGDGYPATNGFVPEIEGANNQTPAAPANPEKARKLLAQ
                     AGYANGANFPATTIYVNGVEGSRSEALIKGYCEVIKRELNIDLSIKLCSLTEREAAIK
                     NGEAKIWRAGWVADYPSPITFLELFYSSGNATNDFKYNNAEFNKTLELSQLEKDSRKK
                     GFLFIKAQSLIAADAVVSPLVVNNMLFMVNARVKGLKANTLEQLNFKDAYIKPSVKEE
                     NE"
     gene            2587..3024
                     /locus_tag="PKN22_03295"
     CDS             2587..3024
                     /locus_tag="PKN22_03295"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNS41761.1"
                     /translation="MAKQKKYTLEIDDEKTAFELIGISSHHNDYRLAWNINNTLNYKF
                     VHSENPFIITFTKKNEIIHQSHTMYEYYDEENRVNFSLIKNKEKGKFLIPERSSIDFF
                     LFIEENEVIDTHQLSEKLRKVESILATFVLDCDTLPSTKHIIF"
     gene            3029..4447
                     /gene="pyk"
                     /locus_tag="PKN22_03300"
     CDS             3029..4447
                     /gene="pyk"
                     /locus_tag="PKN22_03300"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNS41762.1"
                     /translation="MKRTKIVATLGPSTAKKETLKKMFLAGLNVCRLNFSHGGYEAHI
                     ESIKNIRELNEELALNVAILADLQGPKIRTHDMQNNGVLLEKDAIVKITTKKVLGTAK
                     HFSINYEQLPQDVNPGERILLDDGKLALEVTETNKKDAFKAKVIQGGVLSSKKGVNFP
                     NTAISMPSLTEKDREDLNFALEHGVDWIGLSFVRNPEDVRQLKNIIAHREAKAKVIAK
                     IEKPEAVEAIEEIIKEADALMVARGDLGVEVPFQNVPLIQKMIINRARMNAKPVIVAT
                     QMMESMISNLMPTRAEVNDVANAVLDGADAVMLSGETSVGAYPVEVIQTMSDIIGKME
                     SFEGMYNKEEAPEKNQARFITDSICFNACRLAQRTDADAILTMTFSGYTAYKLASQRP
                     KCPIYVFTSNRSILTQINLIWGVKGFYYDKRISTNQTISDIKNILKDKGLLKDGELVI
                     NITSIPLEDLGKSNMIKLSYVD"
     gene            5105..5236
                     /locus_tag="PKN22_03305"
     CDS             5105..5236
                     /locus_tag="PKN22_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41763.1"
                     /translation="MEQHYKQQEEMVAGVLEPKILIAIVIKVIMELVELVVLVLEEL"
     gene            5371..>7430
                     /locus_tag="PKN22_03310"
     CDS             5371..>7430
                     /locus_tag="PKN22_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNS41764.1"
                     /translation="MGYNMQGGNGNGPNSGKGGNGITGDICSDCSRRYNGNNGAVYGA
                     GGGGSLIHLNNWASSWQYATGGNGANGVVIITYTSSSPTASASSNSPICAGQTLTLTG
                     SATGGSPGYTYSWTGPNGFTSTLQNPTIPNATTAATGTYTLVVTDAAGCTDTKTVNVT
                     VNTTPTIDPGSNSPICAGQTLNLTAGGTASTYSWTGPNGFTSSVQNPTISNATTAATG
                     TYTLVASNGTCSATDNVSVTVNPSPTATAGSNSPVCTGKPINLTSSGGGTYSWTGPNG
                     FTSSSQNPSIPSAVSANGGTYTVTVTGANGCTSQATTSVTVNNSPNPSAGSNSPVCQG
                     STINLTSSGAGLFGSYSWTGPNGFTSNSQNPSISNATASHAGTYTVTATNFSGCSAQA
                     TVNVIVNPSPTATAGSNAPICVGSTLNLTANGGSSYSWTGPNGFTSSSQNPSIPNVTA
                     ANAGMYTVTVTQSGCSSTATVDVSINPLPTVTVNSNNPCVGDNINLTSSGGVSYSWTG
                     PNGFTSTLQNPTIPNATASNAGTYTVIVTGANGCTNTGNVVVSVNTAPTASGNSNSPV
                     CVGQAINLTSSGGSSYSWTGPNGFTSNQQNPSISSATASNAGTYTVTVSNGSCSSQAT
                     VNVVVNSLPDPGATAAPVTICVGNTITLNGTGGVSYSWTGPNGFTSSSQNPTIPNATT
                     AASGT"
BASE COUNT         2581 a         1234 c         1436 g         2179 t
ORIGIN      
        1 gtttggcaca tggatttaaa gacaaagcag atcgtttggg ggatgtcaat ggtggattgt
       61 ttacctaccc aatgttgatg gcagcagata ttttaattta cgacgctgaa tttgttcctg
      121 ttggaaaaga tcaagagcaa cacattgagt ttacgcgcga tgtggcttca cgttttcatg
      181 cccaattcaa agaaactttt gtaataccag aagggcttgt taatgaaaac acgaaaatca
      241 ttccgggtac cgatggtcag aagatgagta aatctcgcaa caacttcatt aacatctttt
      301 tacccgacaa acagttgcgc aagcaaatca tgtcgattca aacagatagt aaggaattag
      361 aagagcctaa agatccagca acatgtaatg tttttgcgtt atataaactt ttagcaacgg
      421 acgaacaaat tcaaaacatg accgaattat atgtgaatgg aggatatggc tatgggcatg
      481 caaaacaagc attatttgat ttaattgttg aaaagtacaa aaatcagcgt gagcgttatt
      541 ggtatttaat ggagcaccct gaagaaatta ttgcagcact aaaaataggc gaagacaaag
      601 caagaaagta tgcaaaaaca gttttagagc gtgttcgtgc aactgttggt tattagaaat
      661 ttaactttta aaaagcacac tatctttcag aatattaaca aattatacat caattgattt
      721 aaaaaaaatt aaacaaaacg tcgtttaatt taaatatttt tatatttgtg gtttaaatct
      781 taaatctttc attgaaaatg aagtcaatta tttatgttac actttctact tttttactat
      841 taacttcttg tggtaataaa gaaacaaaaa ctaaagatat tacactaatt aatggcggta
      901 agacgtatgg tggtgagctc gtattttcaa gttctgaagt tgtatccgaa acattcccta
      961 tagcttctgc aaatgtaaac gtcattaata tttcttctca gatttacgaa actttattaa
     1021 atattgatcc tacaactttg gaggttgtac caggtattgc aagtgaattt tcatctaatg
     1081 aagaaggaaa tgtttacaca tttaaaattc gtaaaggaat attttttcac gatgacgagt
     1141 gttttggtgg aaaaggtaga gaattaacac ctgaagatgt caaattttct ttagattttg
     1201 tatgttctgg taatcctttg aatcaagatg acgatatttt ggttaataga attaaaggag
     1261 caaaagaatt caagtcaaaa tctgcaacat cttttccaaa aggtggtgta tctggaatca
     1321 aagtaaaagg cgatgctgtt gaaattactt taaataaacc tttcattgga tttgaagagc
     1381 ttttaacaag aaaaaacact attattttcc caaaagaagc atttgaaaaa tatggtaatg
     1441 atattaaaac acatcctgta ggaacagggg ccttcaaact tgaatcaatt gataaatcac
     1501 aaattcgact agtaagaaac gataattatt ggcaaaaaga tgaatttgga aatcaattac
     1561 cttatttatc agctgttgta ctaaaattca ttcctaacaa aaaggatgaa atgctggcat
     1621 ttagaaacaa aaacattgat ttattgttag atattcctgc tgcagaaatt gaaaatgtat
     1681 tgggtacttt acaagatgca atggctggta aaaatgtgaa acataaagtt gagtcttcac
     1741 aaagtttaaa cattgaatat gttggattta atactgctga tggtgtattt gcttcaaaag
     1801 aagttagaga ggcattcttg tatgctgtta acccttcaga attgattgaa aaatatattg
     1861 gtggtgatgg atatccagca acaaatggct ttgttccaga aattgaagga gccaacaacc
     1921 aaactccagc agcacctgca aatcctgaaa aagcgagaaa attgttggct caagcaggtt
     1981 atgcaaacgg agctaatttc ccagctacca ccatttatgt aaatggagtt gagggttcta
     2041 gaagcgaagc attgataaaa ggttattgtg aagttattaa acgtgagtta aatattgatt
     2101 tatcaattaa actttgttca ctaacagaac gtgaagcagc aatcaaaaat ggcgaagcga
     2161 agatttggag agctggatgg gttgcagact acccaagtcc tattaccttt ttagaacttt
     2221 tttatagcag tggtaatgca acaaatgatt tcaagtataa caatgctgaa ttcaataaaa
     2281 cattggaatt atcacagtta gaaaaagaca gtagaaaaaa aggattttta ttcataaaag
     2341 cgcaatcatt aattgctgcg gatgctgttg tttcgccatt agtggttaac aacatgttat
     2401 ttatggtaaa cgcacgtgtt aaaggattaa aagcaaatac attagaacaa cttaatttta
     2461 aagatgcata cattaaacct tcagtaaaag aagagaatga ataaaaaatt atgttacctt
     2521 aaaaaaggtg cgtaattttg atgcatattt ttaatattaa gttttatcgt ttttttcatc
     2581 aaagctatgg ctaagcaaaa aaaatatacc cttgaaatag acgatgaaaa aacagcattt
     2641 gaattaatag gaattagtag tcaccacaac gactaccgtt tagcgtggaa tataaataat
     2701 acgttaaact ataaatttgt tcattcagaa aaccccttta tcattacttt cactaaaaag
     2761 aatgagatta tacatcaatc tcacacaatg tatgaatact atgatgagga aaatcgtgtc
     2821 aatttttctt tgattaaaaa caaagaaaaa ggtaagtttt taatacctga aagatcctcg
     2881 attgattttt tccttttcat agaagaaaat gaagttatag acacacacca attaagtgaa
     2941 aagttaagaa aagtagaaag tattttagct acctttgtgt tagactgtga cacactacct
     3001 tctaccaaac acattatatt ttaaaaaaat gaaacgaaca aaaattgttg caacattagg
     3061 tccatcaaca gcaaaaaaag aaacattgaa gaagatgttt ttagctggac taaacgtttg
     3121 cagattaaat ttctcacacg gtggctatga agcccacatt gaatctatta aaaatattcg
     3181 cgaattaaac gaagaactag cattaaacgt ggcgatttta gccgatttac aaggtccaaa
     3241 aatccgcaca cacgacatgc aaaacaatgg tgtgttattg gaaaaggatg ctattgtaaa
     3301 aattacaacc aaaaaagttc ttggtacagc caaacatttc tcaattaatt atgagcaatt
     3361 accacaagat gtaaatccag gtgaacgcat tttgctcgac gatggaaaat tggctttaga
     3421 agtgaccgaa accaacaaaa aagatgcttt taaggcaaaa gttatccaag gaggcgtttt
     3481 atcttctaaa aaaggtgtta actttccaaa tacagctatt tcaatgcctt ctttaacaga
     3541 aaaagataga gaagatttga attttgcatt ggaacatggt gtggattgga ttggtttatc
     3601 gtttgttcga aatccagagg atgtacgcca acttaaaaac atcattgcac atcgtgaagc
     3661 aaaagcaaaa gtaattgcaa aaattgaaaa accagaggct gttgaagcca ttgaagaaat
     3721 tatcaaggaa gcagacgcat tgatggtggc gcgtggtgat ttgggtgttg aagtaccttt
     3781 tcaaaacgta ccgttgattc aaaaaatgat tatcaatcgt gcgcgtatga atgccaagcc
     3841 tgtaattgtt gctacgcaga tgatggaatc gatgatttct aacctgatgc caacaagagc
     3901 agaagtaaac gacgttgcca acgctgtttt ggatggtgca gatgcagtta tgttgtctgg
     3961 agaaacatcg gtgggtgctt atccggtaga agttatccaa acaatgagcg atattattgg
     4021 aaaaatggaa tcttttgaag gcatgtacaa caaagaagaa gctccagaaa aaaaccaagc
     4081 gcgtttcatc accgattcta tttgctttaa tgcctgtaga ttagcacaac gcaccgatgc
     4141 tgacgccatt ttaacaatga cattttctgg atacacagcc tataaattag cttcacaacg
     4201 tccaaaatgt ccaatttacg tgtttacaag taacagaagc attttaacgc aaatcaactt
     4261 aatttggggc gtgaaagggt tttattacga caaacgcatc tctaccaacc aaacaatttc
     4321 agatattaag aatattttga aagacaaagg tttattgaaa gatggcgaat tggtgattaa
     4381 cattacttcc attccactcg aagatttggg taaatccaac atgataaaat tgagttatgt
     4441 agattaatct cttcataacc atcatttcct aaatcataag aaaaatacgg ctatccaaga
     4501 atgttaatat ttttggttgg aacaattatt ttgttttttt attaacacat tattttcaca
     4561 aacaactaat attcagtatt ttacgttttt ttaaaatcca aatcatttcg atttttgaac
     4621 aagcataaag aaaagcacta aatttgcgtt tttaaaacta ttatttttta atttactatt
     4681 tctactatta tggaaaagtt aaaactttta tttggattaa tatccatttt cttaagcctt
     4741 tttgttaatg ggcaaacaac agtaacatat acaacaaatg gttcatagac agttcctccg
     4801 tgtgttactt cagttacagt acaagtaata ggtgctggtg gtggtggtgg tggtactgca
     4861 tcacgaaacc cttcaaactc ttcaggttac cctgaaaatg aagcatgtag tgcaggcgct
     4921 ggtggtggtg ctggtggtta tgctagtcgt acatacactg taacacctgg tgatgtttat
     4981 acaattgttg taggaacagg tggagcaggt ggtgtaggcg ctggttctgg gaccacaggc
     5041 accaacaaaa acaatggtgc aaatggaggt ttaagttcat ttgctggacc tgcaactgtt
     5101 ttagttggag caacattaca agcaacagga ggaaatggtg gcgggggtgc tagaacccaa
     5161 aatactaatt gcgattgtca tcaaggtgat aatggaactg gtggaactgg tggtgttggt
     5221 tctggaggaa ctttaaatta tactggagga aatgggtcaa atggtgctca tagtggaagc
     5281 tgtactgaca gaagtggtgc aggcggtggt gctggtggtg ctagtggtaa tggttttaat
     5341 ggtgagtatg ctccttgtcc aaatccatca atgggataca atatgcaagg aggtaatggt
     5401 aacggaccaa actctggaaa aggtggaaac ggtataacag gagatatctg ttctgattgt
     5461 tcaagaagat acaatggaaa taatggagct gtatatggag ctggtggtgg aggttcttta
     5521 attcacttaa ataactgggc tagtagttgg caatatgcaa cgggaggaaa tggtgctaat
     5581 ggtgttgtta tcattactta tacatcatca agtcctacag cttctgcatc ttcaaatagc
     5641 ccaatatgtg caggacaaac attgacatta acaggaagtg caacaggtgg ctcacccggc
     5701 tacacctata gttggacagg accaaatgga tttacatcta cattgcaaaa tccaactatt
     5761 ccaaatgcaa ctacagctgc aactggaaca tacacattag ttgtaaccga cgctgcaggt
     5821 tgtacagata caaaaacagt gaatgtaact gtaaacacga caccaacaat agacccaggg
     5881 tctaatagtc caatttgtgc aggtcaaaca ctcaatctaa cagctggtgg aacggcttct
     5941 acatatagtt ggacgggtcc aaatggattt acaagttcgg ttcaaaaccc aacaatatca
     6001 aatgcaacta cagctgcaac tggaacatat actttagttg cttcaaatgg aacgtgtagt
     6061 gcaactgata atgtatcggt tactgtaaac ccaagtccaa cagcaacagc aggatcaaac
     6121 agccctgtat gtactggaaa accaattaac ttaacatctt ctggaggtgg aacatatagt
     6181 tggacgggac caaatggatt tacaagctcg tctcaaaatc catctattcc ttcagctgta
     6241 tcagcaaatg gaggtacata tacagtaaca gttactggtg caaatggatg tacaagtcaa
     6301 gctactactt ctgtaacagt aaacaattca ccaaatcctt ctgcagggtc caatagccct
     6361 gtatgtcaag gttctactat taatttaact tcaagtggcg caggtttatt tggctcttat
     6421 agttggacgg gtccaaatgg atttacaagc aattctcaaa atccatcaat ttcaaatgca
     6481 acagcatccc atgctggtac atatacggtt acagcaacaa atttttcagg atgttctgct
     6541 caagctacag tgaatgtaat tgttaaccct tcaccaacgg caactgctgg ttcaaatgcc
     6601 ccaatttgtg ttggatctac attaaattta actgcaaatg gaggttcaag ttacagttgg
     6661 acaggaccga atggatttac aagttcgtct caaaacccat caattcctaa tgttactgct
     6721 gctaatgcag gaatgtacac agtaacagta acacaaagtg gatgtagctc aactgctact
     6781 gttgatgttt caatcaaccc attacctact gttaccgtta actcaaacaa tccatgcgta
     6841 ggcgataata tcaatttaac ttcttctggt ggagtttcat atagttggac aggtccaaat
     6901 ggatttacat ctacattgca aaaccctaca attccaaatg caacagcatc caatgctggt
     6961 acatatacag taatagttac aggtgctaat ggttgtacaa acacaggaaa tgtagttgta
     7021 agtgtaaata ccgctcctac agcatctgga aattctaata gtccagtttg tgtaggtcaa
     7081 gctattaatt taacgtcaag tggtggaagt tcatatagct ggactggacc aaacggattt
     7141 acttctaacc aacaaaatcc ttctatttca agtgcaactg catccaatgc tggtacatat
     7201 acagttactg taagcaatgg ttcttgtagt agtcaagcta ctgtaaatgt tgttgtaaat
     7261 tcacttccag acccaggagc gacagctgca ccagtaacaa tttgtgtagg aaatacaatt
     7321 acattaaacg gaacaggagg agtttcatat agctggacag gaccgaatgg atttacaagt
     7381 tcatctcaaa atccaacaat tccaaacgct acaactgcag caagcggtac
//
LOCUS       DAOQVQ010000066         7338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11153_length_7338_cov_3.879445, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000066 DAOQVQ010000000
VERSION     DAOQVQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11153_length_7338_cov_3.879445"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="paaC"
                     /locus_tag="PKN22_03315"
     CDS             complement(<1..425)
                     /gene="paaC"
                     /locus_tag="PKN22_03315"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNS41765.1"
                     /translation="MNQNLYNYLVRLGDDSLILGHRLSEWCGHGPILEEDIALTNFAL
                     DLIGQAQSILEYAGKVEGNGKSADDLAYLRYEHEFRNALLVEQPNGDFGVTMMRQFLF
                     DAFRKPLFEALTKSKDEQIAAIAEKSLKETRYHLKHSSE"
     gene            complement(438..728)
                     /gene="paaB"
                     /locus_tag="PKN22_03320"
     CDS             complement(438..728)
                     /gene="paaB"
                     /locus_tag="PKN22_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNS41766.1"
                     /translation="MSENANWPLWEVFISSKAGMSHKHAGSLRAADAKMAIENARDVY
                     TRRSEGTSIWVAKSNDVFASDPNDADAFFDPANTKPYRHPTFYVIPDGVKNM"
     gene            complement(776..1717)
                     /gene="paaA"
                     /locus_tag="PKN22_03325"
     CDS             complement(776..1717)
                     /gene="paaA"
                     /locus_tag="PKN22_03325"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNS41767.1"
                     /translation="MNIEELERQFQEKIDNEIKIEPKDWMPDAYRNLLIRQISQHAHS
                     ETVGMLPEGNWITRAPSLRRKQILLAKVQDEAGHGLYLYSACETLGVDREDTIDDMHS
                     GKAKYSSIFNYPALTWADMGAIGWLVDGAAIMNQIPLSRCSYGPYARAMVRVCKEESF
                     HQRQGYEIMTHLANGTPAQKAMAQDALNRFWWPAMMMFGPSDNDSKHSQQSMKWKIKR
                     FSNDELRQQFVDATAPQAALIGLTVPDPDLKFNEATGHYEFGQMNWDEFWQVVNGNGP
                     CNKERLDARRKAKENGMWVREAAAAYAKKQEEKAVVA"
     gene            1893..2972
                     /locus_tag="PKN22_03330"
     CDS             1893..2972
                     /locus_tag="PKN22_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNS41768.1"
                     /translation="MGFFGNLMKKDTVDKTPKGFHHLTVKKINRLTPDSVEVVFSIPD
                     DLKNEYSFVPGQYVTLDITIDGKEERRSYSICSKKGEDLAIAVKEVEGGKVSPLINKE
                     LSEGQTLLVSKPEGRFMMKEVKNIVGIAAGSGITPIISMAYEIEDSEGCSMKLLFANK
                     TENDIIFRDRLDRFQKTQKRYFLTQEKKEGFDYGRITKDAFVNFIKEDLDVLKSDGFY
                     ICGPEEMINNVKEALVLFGVPESKIHFELYVVSTESDKKATKEVVGYQGIAQVKAIID
                     DDVYEFEMDGSKMSVLDAALDADADAPYSCKGGVCCTCRAKVLEGNAIMKLNYTLTEE
                     EVNQGYVLTCQAIPTTPTLTVSYDD"
     gene            2965..3654
                     /locus_tag="PKN22_03335"
     CDS             2965..3654
                     /locus_tag="PKN22_03335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS41769.1"
                     /translation="MTKTIVNIGSSRGIGKSLIDYLAKNETYKIYALSRNEELMRKQF
                     ASYSNVECKFFDLNANDLTSQLDYIFSQVDKIDVLINNAGYLVNRPFEQLSRRDFELS
                     YQVNVIGVMETIQHLLPKLEHGAHVVNISSMGGFQGTVKFAGLAAYSTSKAALVCFTE
                     LFAEEFKNSAIKMNCLCLGAVQTEMLQEAFPGYEALVSPDKMAEYVGNFALTAHQWMN
                     GRIIPVSLSTP"
     gene            3696..4778
                     /gene="dinB"
                     /locus_tag="PKN22_03340"
     CDS             3696..4778
                     /gene="dinB"
                     /locus_tag="PKN22_03340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNS41770.1"
                     /translation="MRKIIHIDMDAFFASVEQRDDKSLQGKPIAVGSSGKRGVISTAS
                     YEARKYGIRSAMPSQIAIKKCPELIFVPPRYDVYKSVSNQIRQIFLEYTDLVEPLSLD
                     EAYLDVTTNFKNIPFAIDVAKEIKDRIFQTTQLTASAGVSINKFLAKIASDYNKPNGL
                     FIIGPKKAEKFVESLPIEKFFGIGEKTAIKMHQMNIFTGKDLKERSEEFLITNFGKSG
                     KTYFLNARAIDNRAVVSNRQRKSLGVEWTFTEDVSSLLQVENELIAICEELANRIAKS
                     KFKGRTISLKLKLEDFSILTRSKTLPNDIEEFSVLVESSKEMLNQIELKQKIRLIGVS
                     VKASESVGEVYEVDRQMMIPFEEDRG"
     gene            4911..4992
                     /locus_tag="PKN22_03345"
     tRNA            4911..4992
                     /locus_tag="PKN22_03345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4945..4947,aa:Leu,seq:caa)
     gene            5063..5779
                     /gene="bshB1"
                     /locus_tag="PKN22_03350"
     CDS             5063..5779
                     /gene="bshB1"
                     /locus_tag="PKN22_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNS41771.1"
                     /translation="MKVDILAFAAHPDDIEISMSGTILKHIAAGKTVGIVDLTRGELG
                     TRGTVKTREEEARQATKLMGLHYRNNLDLGDGVFENNLEARLSIIREIRKCQPEVVFA
                     NSITDRHPDHGRASKLVSEACFYSGLVKIETQYDGQIQKAHRPNAVYHYIQDRYIKPD
                     FVVDVTPFVDQKIDILKTYSTQFYNPNSEEPSTPISGKEFFDFIKGRMSEFGRPIGVP
                     FAEGFTAERLIGVEDIFQLK"
     gene            complement(5780..6178)
                     /locus_tag="PKN22_03355"
     CDS             complement(5780..6178)
                     /locus_tag="PKN22_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS41772.1"
                     /translation="MGATKHEVHSTEINELASLFKALSNPARVKALLMLAESEQKTVT
                     TEDFQNEIELSQSSISRHLNILSDSGLIATKIITRNKKSCLAYQLNYEGIQILQQVIS
                     KITLMSGNRNTNNFYSKYKNILNTSLLFKT"
     gene            complement(6306..>7338)
                     /locus_tag="PKN22_03360"
     CDS             complement(6306..>7338)
                     /locus_tag="PKN22_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNS41773.1"
                     /translation="LTPGHALIIKKSGKVSEVLINEPKVAKKCSFERIYFARNNDADI
                     YKERQLLGNLIIPQVLKTIDYDLDNTVFSFIPNTAETSFYGMIKGLEDFLNIKKLERI
                     TALGNDISKEKLEEILFQRARIEKIINKDAKVRTFISESESRDDLVSSSYDITYGSVK
                     RGQDNLVVIDDSIVRGTTLKQSVLRILDRLDPKKIVVVSSAPQVRYPDCYGIDMTKMS
                     EFVAFVAAIELLKESGRQHVIDDVYRKCKEQEHLPKEQIKNYVKEIYAPFTDDEITQK
                     IVKILTPNDLKADFDIVYQSVENLNKACPNDLGDWYFTGNYPTPGGNKVVNKAFINWV
                     EGINERPYL"
BASE COUNT         2422 a         1158 c         1278 g         2480 t
ORIGIN      
        1 cattcagaag aatgttttaa atggtaacgt gtttctttta atgatttttc agcaatggct
       61 gcaatttgtt cgtctttact ttttgttaaa gcttcaaaca aaggttttct gaatgcatca
      121 aataaaaatt gacgcatcat tgttactcca aaatcaccat tgggttgttc aaccaataaa
      181 gcatttctaa actcatgttc gtaacgcaaa taagccaaat catcagcact ttttccgttt
      241 ccttcaactt ttcctgcgta ttctaaaata gattgcgcct gaccaattaa atccaatgca
      301 aaattagtca atgcaatatc ttcttccaag attggaccgt gtccacacca ttcagaaaga
      361 cgatgtccaa gaataaggct atcatcgccc aaacggacta aataattgta taaattttga
      421 ttcattttag ccttatttta catgttttta actccgtcgg ggataacata aaacgtaggg
      481 tgacggtatg gttttgtatt tgcaggatca aagaatgcat ctgcatcatt tgggtcagaa
      541 gcaaaaacat cgtttgattt agcaacccaa atacttgttc cttccgaacg acgtgtatat
      601 acatcacgtg cattttcaat agccattttt gcatcggcag ctctaagact tcctgcatgt
      661 ttatgactca ttcctgcttt actactaata aaaacctccc ataaaggcca atttgcattt
      721 tcagacatat aaatattttt ttatcgatta aataaattca attttctact taggattagg
      781 caacaacagc tttttcctct tgttttttag cataagcagc agcggcttca cgcacccaca
      841 taccgttttc ttttgctttt cttcttgcat ccaaacgctc tttgttgcac ggaccatttc
      901 cgtttacaac ttgccagaat tcgtcccagt tcatttgtcc aaattcataa tgacctgtag
      961 cctcgttgaa ttttaaatct gggtctggaa ccgttaaacc aattaacgct gcttgaggag
     1021 ctgttgcatc tacaaattgt tgacgtaatt catcgttaga aaaacgttta attttccatt
     1081 tcatagattg ttgtgagtgt ttagaatcat tatcacttgg tccaaacatc atcattgcag
     1141 gccaccaaaa acggttcaaa gcatcttgag ccattgcttt ttgagcaggt gttccatttg
     1201 ccaaatgcgt cataatttca tatccttgac gttggtgaaa agattcttct ttacaaactc
     1261 tcaccattgc acgagcataa ggtccgtatg aacatctaga aagtggaatt tggttcataa
     1321 tagcagcgcc atcaaccaac caacctattg ctcccatatc agcccatgtt aatgcagggt
     1381 agttgaaaat agaagaatat tttgcttttc cagagtgcat gtcatcaatt gtatcctctc
     1441 tgtcaacacc taaagtttcg caagcactgt ataaatacaa accatgacca gcctcatctt
     1501 gcactttagc caataaaatt tgttttcttc ttaagcttgg agctcgagta atccagttac
     1561 cttctggcaa catccctact gtttcagaat gtgcatgctg agaaatttga cgaatcaata
     1621 aatttctgta ggcatctggc atccagtctt taggctcaat tttaatttca ttatcaattt
     1681 tttcttgaaa ttgacgttct agttcttcaa tgttcataaa ttttaccttt ttatcctaat
     1741 tacaacaaaa ctataaaaat aattgttttt aatgtttaaa tatacaaact tcattaggtt
     1801 tttaacacaa atagtcaata tagttagttt ttatattatt gaataatttg cataaaatga
     1861 ctatatttgc cccctaaaac aatagctata aaatgggatt ttttggaaat ttaatgaaga
     1921 aagatacggt tgataaaacg cctaaagggt ttcatcattt aaccgtaaaa aaaataaata
     1981 gattaacacc cgattctgtt gaggtggttt tttcaattcc agacgattta aaaaatgaat
     2041 attcgtttgt tcctggtcaa tatgttacac ttgacataac aattgatggt aaggaagaaa
     2101 gacgttctta ttcaatttgc agtaaaaaag gagaagattt agctattgca gttaaagaag
     2161 tggaaggtgg gaaagtttct ccattaatta ataaagaact ttcagaagga caaactttat
     2221 tggtttcaaa accagaaggg cgtttcatga tgaaggaagt taaaaatatt gttggtattg
     2281 cagctggttc tggaattacg ccaattatca gtatggctta tgaaattgaa gattctgaag
     2341 gatgttcaat gaaattatta tttgccaaca aaacggaaaa tgacattatt tttagagatc
     2401 gattggatcg ttttcaaaaa actcaaaaac gctatttctt aacacaagag aaaaaagagg
     2461 gatttgatta tggtcgtatc acaaaagatg catttgtcaa tttcattaaa gaagatttag
     2521 atgtattgaa atctgatgga ttttacattt gtggtcccga agaaatgatt aacaatgtta
     2581 aagaagcatt ggttttgttt ggggttccag aatctaaaat tcattttgaa ttgtatgttg
     2641 ttagcacaga gtcagataaa aaagcaacta aagaagttgt cggttatcaa ggcattgccc
     2701 aagtaaaagc aataattgac gatgatgtat atgagtttga aatggacggt agtaaaatgt
     2761 ctgttttaga tgcagcacta gatgcagatg cagatgctcc ttattcttgc aaaggtgggg
     2821 tttgttgtac gtgtagagca aaagtattag aaggaaatgc aataatgaaa ctcaattata
     2881 cattaactga agaggaggtt aaccaaggtt atgtgcttac atgtcaggca attccaacta
     2941 caccaacgtt gacagtttcc tacgatgact aaaacaattg taaatattgg ttcttcgcga
     3001 ggcataggta agtcacttat agattatcta gcaaaaaacg aaacgtataa aatttatgcg
     3061 ttatcacgaa acgaagagct gatgcgaaaa caatttgcta gttattcgaa tgtagaatgt
     3121 aagtttttcg acttaaacgc caacgattta acgtcacaat tggattatat tttctcacag
     3181 gttgataaaa ttgatgtgct catcaataat gctggatatt tggttaatcg tccgtttgaa
     3241 caactttcta gaagagattt tgaattgtcc tatcaagtaa atgttattgg tgtgatggag
     3301 actattcagc atttgcttcc aaagttagaa catggagcac atgtggtgaa tattagctca
     3361 atgggaggtt ttcaaggaac tgtaaaattt gcaggattgg ccgcatattc aacctctaaa
     3421 gctgcattgg tttgttttac tgaattgttt gcagaggaat ttaaaaattc tgcaataaaa
     3481 atgaattgtt tgtgtttggg agctgtgcaa actgaaatgt tgcaagaagc ttttccaggt
     3541 tatgaagctc ttgtttcacc agataaaatg gcggagtatg ttggtaattt tgctttgact
     3601 gctcaccaat ggatgaatgg gagaattata ccggtatctt tatctacacc ataaatataa
     3661 actactgatt tatcacactt ttgaggagtg ttgttatgcg taaaattata catattgaca
     3721 tggatgcgtt ttttgcatcg gtagagcaac gtgacgataa atcattacaa ggaaaaccca
     3781 ttgcagttgg ttcaagtgga aaacgaggag ttatttcaac cgctagttac gaagcacgaa
     3841 agtatggaat tcgctcggca atgccctctc aaattgccat caaaaaatgt ccagaactga
     3901 tatttgttcc accacgttac gatgtttata aatcggtatc caaccaaatt cgtcaaattt
     3961 ttttggaata cacagattta gtcgagcctt tatcactaga tgaggcatat ttggatgtta
     4021 ccactaattt taaaaacatt ccttttgcaa ttgatgttgc aaaggaaatt aaagatagaa
     4081 tatttcaaac tactcaattg acagcatctg caggtgtttc tatcaataaa tttttagcaa
     4141 aaattgcttc ggattacaat aagcccaacg gactttttat tattggccct aaaaaagcag
     4201 aaaaatttgt tgaatcgcta cctatcgaga aattttttgg aattggagaa aaaacggcaa
     4261 tcaagatgca tcaaatgaac atctttacag gtaaagattt aaaagaacga tctgaagaat
     4321 tcttgattac taattttggg aaatcgggta aaacttattt cttaaacgca agagcaatag
     4381 ataatcgcgc agtagtttca aatagacaaa gaaaatcttt gggtgtcgag tggacattta
     4441 ccgaggatgt gtctagtttg ttgcaggttg aaaatgaatt gatagcaata tgtgaagaat
     4501 tagctaatcg aatagcaaaa agtaaattta aaggacgaac gattagcttg aaattgaagt
     4561 tggaagattt ttctattctt acaagaagta aaactttacc aaacgatatt gaagaatttt
     4621 cggtgttagt tgagagttca aaggagatgt taaaccaaat tgaattaaag caaaaaatta
     4681 ggttgattgg tgtgagtgtg aaggctagtg aaagtgtagg agaggtgtat gaggttgata
     4741 gacagatgat gatacctttt gaagaagata ggggttgatt gaaatatgtt gtttttatgt
     4801 caatatcgaa atatgtcgat atcagtaaat ttctaaaaaa atcaaatttt ttatcaaata
     4861 gtataaatat atatgatttt ttacttacat ttgcacccag attttgaaaa gcccgagtgg
     4921 cggaactggt agacgcgctg gattcaaaat cctgttcttt cgggagtgcc ggttcgattc
     4981 cggcctcggg tacaaaaaag agattgtcat tttgataatc tcttttttta ttttatattg
     5041 tgtcaacttt ttttgatttt gtatgaaagt agatattttg gcatttgcag ctcatcctga
     5101 tgatattgaa atttcaatgt ctggtactat tttaaaacat attgcagctg gtaaaacagt
     5161 tggtattgta gatttaacaa ggggtgaact tggtacacga ggaacagtca aaacaagaga
     5221 ggaggaggct cgacaagcaa ctaaattaat ggggttgcat tatcgtaata atttagactt
     5281 aggtgatggg gtttttgaaa acaatttaga agctagactt tcaataatta gagagattag
     5341 aaagtgccag ccagaagttg tctttgctaa ctcaataact gatagacatc ctgatcacgg
     5401 tagagcttca aaattagtat cagaagcatg tttttattct ggattggtaa aaatagaaac
     5461 tcaatatgat ggtcaaattc aaaaagctca tagaccaaat gctgtatacc actatattca
     5521 agatcggtat attaaacctg attttgttgt agatgttacc ccttttgttg atcaaaaaat
     5581 tgatattctt aaaacttata gcacacaatt ttataaccct aattcagaag aaccctcaac
     5641 acctatttca ggaaaagagt tttttgactt tataaaaggt agaatgtctg agtttggacg
     5701 tccaattgga gtgccttttg ctgaaggatt tacagctgaa agactaattg gagtggaaga
     5761 tatctttcaa ttaaaataat taggttttaa ataataaaga agtattgagg atatttttat
     5821 attttgaata aaagttattt gtattcctgt ttccactcat tagagtaatt ttagaaataa
     5881 cttgctgtag aatttgaata ccttcatagt ttaattgata tgctaaacaa ctttttttat
     5941 ttcttgtaat aattttagta gcaattaaac cagaatcgga taagatattt aaatgacgag
     6001 aaattgaaga ttgtgataat tctatttcat tttgaaaatc ttctgttgtt actgtttttt
     6061 gctcgctttc agcaagcatt agtaatgctt taactctagc aggattggat aatgctttga
     6121 aaagactagc tagttcatta atttccgtag agtgaacttc atgtttagtt gctcccatat
     6181 gttggattgt aaaaggttaa taaaatatta ctataaatct aataataaaa tttacaattt
     6241 caaagaaaaa atgagagttt tcgcaatatc gaaatatttc gatattgcga aaacatcaga
     6301 aaattctata aataaggtct ctcattaata ccctctaccc aattaataaa agctttattc
     6361 actactttat ttccacctgg agtaggataa tttccagtaa aataccaatc acctaaatca
     6421 tttggacacg ctttattcaa attttctaca gattgataaa caatatcaaa atctgctttc
     6481 aaatcatttg gagttagaat ttttacaatt ttttgtgtga tttcatcatc tgtaaaagga
     6541 gcataaattt cttttacata attttttatt tgctcttttg gtagatgttc ttgttcttta
     6601 cacttacgat atacgtcatc tataacatgt tgacgaccag attcttttaa taattctatt
     6661 gcagcaacaa aagcaacaaa ctcactcatt ttggtcatgt ctattccata acagtcagga
     6721 tatctaactt gtggggcaga tgaaacaacc acaatttttt taggatcaag cctatctaat
     6781 attcttaaaa cagattgttt taaagttgtc cctctaacaa tactatcgtc aatcaccacc
     6841 aaattatctt gtcctctttt aacacttcca taagtaatgt catatgatga agaaacaagg
     6901 tcgtctctgg attcgctttc agaaataaaa gttcttactt tagcatcttt gttgataatt
     6961 ttctcaattc tagcacgttg aaaaagaatt tcttctagtt tttcttttga tatatcattt
     7021 ccaagagcag tgattctttc taactttttg atgtttaaga aatcctcaag ccctttaatc
     7081 ataccataaa acgaagtttc tgcagtgttt ggaataaatg aaaagacggt gttatctaaa
     7141 tcataatcaa ttgtttttag gacttgtgga ataattaaat tacccaacaa ttgacgttct
     7201 ttataaatat cagcatcatt atttctagca aagtaaattc gttcgaacga acatttttta
     7261 gctacttttg gttcattaat caatacttca cttactttcc cagatttttt aataattaag
     7321 gcatgacctg gagttaac
//
LOCUS       DAOQVQ010000067         7334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11158_length_7334_cov_4.056601, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000067 DAOQVQ010000000
VERSION     DAOQVQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11158_length_7334_cov_4.056601"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1030)
                     /locus_tag="PKN22_03365"
     CDS             complement(107..1030)
                     /locus_tag="PKN22_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS41774.1"
                     /translation="MGARLAYYCLVYPISKLPFWCLYALSFKMYVLFRIFPYRKKVIE
                     GNIRRSFPQKSDAEIKKLVRQFYRHFTDILAEGVKNLSISEHELRQRYRVKNPELMDD
                     LYRQGKSVLLVSGHYNNWEWLISAQNFLFPHQAMGIGMPLSSKFWDKKVNERRERFGM
                     QVVHSKNYREKIAAQKEQPIAILTLTDQSPGDARKSYWMDFLNQPTAVLFGAEMMAHE
                     HNFAVVFYRTRKIKRGYYEMELTTITENPKTMSWGAITEAHTHLLEQAIMEVPEQWLW
                     SHKRWKREVPSDLEQLRNEQRQKFESRFVEK"
     gene            complement(1030..2301)
                     /locus_tag="PKN22_03370"
     CDS             complement(1030..2301)
                     /locus_tag="PKN22_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNS41775.1"
                     /translation="MNFNAPLAERMRPKSLDDYIGQHHLLKKDAALRLALDAGVIPSM
                     IFWGPPGVGKTTLAYLIAHHLKRPFHTLSAISSGVKDVREIIQNVERQGMFGGQGTIL
                     FIDEIHRFSKAQQDSLLGAVEKGIVTLIGATTENPSFEVISALLSRCQVYVLESHTKD
                     DLQQLLEKAIAEDELLKTKNIVLEETNSLMAISGGDARKLLNVFEIIVGTFQQQEKIV
                     INNAVVEAVAQQSLVRYDKDGEQHYDIISAFIKSIRGSDPDAAVYWLARMVEGGEDPK
                     FIARRLIISAAEDIGLANPNALLIANNCFQAIQVIGWPEARIILAQCTIYLATSPKGN
                     AAYMAINRAQEEVKRSGNLAVPIPLRNAPTKLMKELGYGEEYKYSHDYPGNFVQQNFF
                     PEELKNTQFFKAGSSSKEQELAAIIKKLWNR"
     gene            2428..3099
                     /locus_tag="PKN22_03375"
     CDS             2428..3099
                     /locus_tag="PKN22_03375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS41776.1"
                     /translation="MSFTILTLIIAITVIVSISAFQNQDKLYRSLFIPARIQETKEYY
                     RFFSHLFVHADYGHLIFNMLSLYFLGSFMLDTPGGTYMDANGGLVEVQDGWMQMYGYL
                     SGQVMFGVLYFAGGLFATIIPYMRNKNNPNYRSLGASGAVSAVIFAAILWNPTMKLNI
                     LFIPIGIPAYIFGPLYIAYEIYMDKRGGTGVAHDAHLGGALFGILFVLFTNIEVGKRF
                     LELIF"
     gene            3101..3964
                     /locus_tag="PKN22_03380"
     CDS             3101..3964
                     /locus_tag="PKN22_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNS41777.1"
                     /translation="MLYVNNNDHILPVEEYSIHAGSRAYLYGDGVFESIRIINGTPIN
                     LENHIKRLLEGAKAIRLRVPSYFNLDFFAVRIKELCEKSEIRQGGYCRLSLDRMSGGR
                     FKPETNECVFFIEVKPIDTNFFNLNAKGLEVDIFTDLKKQKNMLANFKTKNCLLYIMA
                     SIKAKENQLDDLLITNDNGGILESSSSNMFVVSNNILYTPGLEEGCLAGTMRMQIINL
                     ALRNGFRVYESNITPQNLLIADEIFLTNAISGITWVSGYRTKRYFNNISKRLNQLLNE
                     HWEETLKNKPE"
     gene            4001..5365
                     /gene="radA"
                     /locus_tag="PKN22_03385"
     CDS             4001..5365
                     /gene="radA"
                     /locus_tag="PKN22_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNS41778.1"
                     /translation="MAKQKSAYFCQNCGYEAPKWLGKCPSCNEWNTFVEELIEKNLPG
                     NIVAFSKSGKTSKPTAILDIQGVEQERYQLADKELNRVLGNGLVLGSLVLFGGEPGIG
                     KSTLMLQLAIANTSIPVLYVSGEESEQQIKMRAERIGVKNQSCFILSETNIQNIFKQA
                     EEIEPKLLVIDSIQTLYSSDIESSPGSISQVRECTAQLLRYAKTTNIPVFLIGHITKE
                     GSLAGPKVLEHMVDTVLQFEGDRNHVYRLLRSVKNRFGSTNELGIYEMIGSGLRQVEN
                     PSEILITNADSSLSGISIGATLEGMRPMLIEVQALVSTAAYGTPQRSSTGFDSKRLNM
                     LLAVMEKRCGFKLGAKDVFLNIAGGIKVDDPALDLAVVASVLSSNADIAINKGICLSA
                     EVGLSGEVRPVNRIDQRIYEAEKLGFEKIIISKYNKGIKQSDFKIELVLCSKLDEVVR
                     ALFA"
     gene            complement(5354..6487)
                     /locus_tag="PKN22_03390"
     CDS             complement(5354..6487)
                     /locus_tag="PKN22_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS41779.1"
                     /translation="MINRTLVKNIQNKLFKGKTIVIVGARQVGKTTLIKTVLSGKDYL
                     FLNGDDPYTRSILRNPSTKELENIIGNSKIVFIDEAQRLENIGITAKIIHDQMQNVQL
                     ILSGSSAFELKNKTNEALTGRKFEYFLMPISYEELEQSTSFIDAASNLKNRLVFGMYP
                     EIINHPGEEVVLLNELTQSYLYKDILALGSVQKPDVLEKILRALAFQVGSEVSYNEVA
                     QLVGVDKNTVANYIHLLELSYVIFTLPSLSRNLRNEIKTNKKIYFYDNGVRNALIQNF
                     NPLELRNDVGALWENFLITERMKRNSYHQSFVNRYFWRTVQQQEIDYVEEQNGVIKGY
                     EFKWNPKAKAKQPKAFIEAYQSSIETIHQENYRDFIVYPPSSK"
     gene            complement(6667..7167)
                     /locus_tag="PKN22_03395"
     CDS             complement(6667..7167)
                     /locus_tag="PKN22_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41780.1"
                     /translation="MKKTLLSAVVMGLLVVTACKKEGCMDNTATNFNAEATKDDGSCQ
                     YAPVAQDGFRWKEDGGSEIVADSAFWTTGSWGTGIRAYKGGNSNFFEINWDNQDNTTI
                     GTKTVSSSYGFTFMKNNTTYTNSGSASVAITAFSDNKMSGNFTISVSGGSIQSIQANF
                     GNLPKK"
BASE COUNT         2197 a         1416 c         1355 g         2366 t
ORIGIN      
        1 aaaatgccag ttccgttgca attgattgcc gtttcttgca tcatcttttt gatgtatcaa
       61 gcaatgacgg gcgaatcaaa agcatttgtg tacttccaat tctaatttat ttttccacaa
      121 aacgggattc aaatttttga cgttgctcgt tgcgcaattg ttctaaatca gacggaactt
      181 ctcttttcca acgtttgtgc gaccacaacc attgttcggg cacttccatt atggcttgtt
      241 ccaacaaatg cgtgtgagcc tcggttattg ctccccaact cattgttttt ggattttcgg
      301 taatggtggt aagctccatt tcgtagtagc ctcgttttat cttacgggtg cgataaaaaa
      361 caactgcaaa attatgctcg tgcgccatca tttcggcacc aaacaataca gctgtaggtt
      421 ggtttaaaaa atccatccaa tagcttttgc gtgcatcacc cggtgattga tcggtgagcg
      481 ttaaaatggc aataggttgt tctttttgcg cagcaatttt ttctctataa tttttagaat
      541 gaactacttg cataccaaaa cgttctctgc gttcgtttac ttttttgtcc caaaatttag
      601 aactcaacgg cattccgatt cccattgctt ggtgtggaaa caaaaagttt tgtgcagata
      661 tgagccattc ccaattgttg taatgcccag aaaccaataa aacacttttt ccttgacggt
      721 ataaatcatc catcaattcg ggatttttta cacggtaacg ttggcgcaat tcgtgttctg
      781 aaatgctcaa atttttaacg ccctctgcaa gaatatctgt aaaatgacgg taaaattgac
      841 gcaccaactt cttgatttct gcatccgatt tttgtggaaa cgaacggcga atattgccct
      901 caattacctt ttttcgataa gggaaaatgc gaaacaacac atacatttta aacgaaagcg
      961 catacaaaca ccaaaacggc agttttgaaa tcggataaac caaacagtag tatgccaaac
     1021 gtgctcccat tatctattcc acagtttttt aatgattgct gccaattctt gctctttgct
     1081 actgctccct gctttgaaaa attgagtgtt tttaagttct tcaggaaaga agttttgctg
     1141 tacaaaattg cccgggtaat cgtgtgaata tttgtattct tcgccataac ccaattcttt
     1201 catcaatttg gttggggcgt tgcgcaaagg aataggaacc gccaagttgc cacttctttt
     1261 cacctcttct tgcgcccgat tgatggccat gtaagcagca tttcctttgg gcgatgtagc
     1321 aagataaatg gtgcattgtg ccaaaatgat gcgggcttcg ggccaaccaa taacttgaat
     1381 ggcttgaaaa caattgttgg caatcaacaa agcattcgga ttagccaaac cgatgtcttc
     1441 agcagcactg atgatgagtc ttcgcgcaat aaatttagga tcttcgccgc cttctaccat
     1501 acgtgccaac caataaaccg ccgcatcggg gtcgcttcca cgaatggatt tgatgaatgc
     1561 agaaatgata tcgtaatgtt gctcaccatc tttatcatag cgcaccaaac tttgttgggc
     1621 aacagcttcc accacagcgt tgttgatgac aattttttct tgttgctgaa aagttcccac
     1681 aatgatttca aaaacattga gcaatttgcg tgcatcgccg cccgaaatgg ccatgagtga
     1741 gttggtttct tccaacacaa tgtttttggt tttcaataat tcgtcttcgg caatggcttt
     1801 ttcaagcaac tgttgtaaat catcttttgt gtgactttct aacacataca cttggcaacg
     1861 cgataaaagt gccgaaataa cctcaaacga aggattttct gtggtggcgc caatcaacgt
     1921 aacaattcct ttttctacgg cacccaacaa tgaatcttgt tgtgcttttg aaaaacggtg
     1981 aatttcatca ataaacaaaa ttgttccttg tccgccaaac attccttgtc gctccacatt
     2041 ttgaatgatt tcgcgcacat ctttcactcc cgaagaaata gcagaaagtg tatgaaaagg
     2101 gcgttttaag tgatgggcaa ttaaataagc caaagtggtt tttccaacgc ccggaggtcc
     2161 ccaaaaaatc attgaaggaa tcacacctgc atcaagagcc agacgcaatg ctgcatcttt
     2221 ttttagcaaa tggtgctgac caatgtaatc gtccaacgat tttggtcgca tacgttcagc
     2281 aagcggagca ttgaaattca taatcacttt attctctaca aagatagctt ttgaatttaa
     2341 aaaaacgaga agaacagacg gaattaaaca agataaaaag acactccttt caataaaatc
     2401 actaattttg atgttcatat taaagaaatg agttttacaa tcctaacatt aattattgcc
     2461 attaccgtaa ttgtctcgat ttcagcattt caaaatcaag acaagcttta ccgttctctt
     2521 tttattcctg cacgcataca agaaaccaaa gaatattacc gttttttctc gcacctattt
     2581 gtacatgcag attatggaca tttgatattt aacatgcttt cgttgtattt tttgggtagt
     2641 tttatgttgg acacacctgg aggcacctac atggatgcaa acggcggttt ggttgaagtg
     2701 caagatggat ggatgcaaat gtatggctat ttatctgggc aagtgatgtt tggagtgttg
     2761 tattttgcgg gcggactttt tgccactatc attccctaca tgcgcaataa aaacaatccc
     2821 aattaccgtt cattaggagc atcgggtgct gtttctgccg taatttttgc agcaatattg
     2881 tggaatccga caatgaaatt gaacatactt tttattccaa ttggtattcc agcctacatt
     2941 tttggtccgc tctacattgc ctatgaaatc tacatggaca agcgcggagg cacaggagtt
     3001 gcacacgatg cgcatttagg tggggcactt tttggtattt tattcgtttt atttactaat
     3061 attgaagtcg gaaagcgatt tttggagtta attttttaat atgttatacg tcaacaacaa
     3121 cgatcatatt ttacctgtag aggaatacag tattcacgct ggaagtcgtg cttacctcta
     3181 cggtgatggc gtttttgaaa gtattcgcat catcaatgga acccccatta atttagaaaa
     3241 tcacatcaaa cgcttgttag aaggtgcaaa agctatccgt ttacgagttc cttcttattt
     3301 taacctcgac ttttttgccg ttcgcattaa agaattgtgc gaaaaatcgg aaataagaca
     3361 aggtggttat tgccgtttat ccttggatag aatgtcgggt ggtagattta aacctgaaac
     3421 caacgaatgt gtgtttttta ttgaggtaaa accaatagac actaattttt tcaacctcaa
     3481 cgcaaaaggg ttggaagtgg atatttttac cgatttgaag aagcaaaaaa acatgcttgc
     3541 caacttcaaa accaaaaatt gtttgctcta catcatggcg agcatcaaag ccaaagaaaa
     3601 ccaactggat gatttgctca tcaccaacga taatggtggt attttagaga gttcaagcag
     3661 caatatgttt gtggtttcca acaatatttt atatacacca ggacttgaag aaggttgttt
     3721 ggctggaaca atgcgcatgc aaatcatcaa cttagcgctt cgcaatggtt ttagagttta
     3781 tgaatccaat attacgcctc aaaacttgtt gattgctgac gaaatatttt taaccaatgc
     3841 catttcggga ataacctggg tgagtggtta ccgcaccaaa cgttatttca ataatatttc
     3901 aaaacgattg aaccaacttt taaacgaaca ttgggaagaa acattgaaaa ataagcccga
     3961 ataatcggta tttgattttt caatttatta aattcgttgc atggcaaaac aaaaatcagc
     4021 ttatttctgt caaaattgtg gctacgaagc tcctaaatgg ttgggaaaat gcccgtcgtg
     4081 caatgaatgg aacacatttg ttgaggaatt gattgaaaaa aatcttccgg gcaacattgt
     4141 agcgttttct aaatctggaa aaacttccaa accaactgcc atacttgata ttcaaggtgt
     4201 ggagcaagaa cggtatcaac ttgccgataa agaattgaac cgcgtattgg gcaacggctt
     4261 ggtacttggc tctttggttt tgtttggagg cgaaccgggc ataggaaaat ccactttaat
     4321 gcttcaatta gccattgcca acacttccat tccggtgttg tatgtttcgg gcgaggaaag
     4381 tgaacaacaa ataaaaatgc gtgccgaacg tattggtgtt aaaaatcaat cgtgtttcat
     4441 tctttctgaa accaacattc aaaatatttt taagcaagca gaagaaattg aaccaaaatt
     4501 attggtaatc gactccatac aaacacttta cagttcagat attgagagtt ctcctggaag
     4561 tatttcgcaa gtgcgcgaat gtactgctca attgttgcgt tatgccaaaa ccaccaatat
     4621 tccggttttt ttaattgggc acatcaccaa agaaggttca ttggccggac caaaagtatt
     4681 ggagcacatg gtggatacag ttttgcaatt tgaaggcgat agaaatcacg tttaccgttt
     4741 gttgcgctcg gtgaaaaatc gttttgggag caccaacgaa ttgggaatat acgaaatgat
     4801 aggttcgggc ttacgtcaag tggaaaaccc ttccgaaata ttaattacca acgccgatag
     4861 ttcgctcagc gggatatcta ttggtgctac gttggaaggc atgcgcccta tgttgattga
     4921 agtgcaagcg ttggtgagca cagcagctta tggtacacct cagcgttcct ccactggttt
     4981 tgatagcaag cgattgaaca tgttgttggc agtaatggaa aaacgttgtg ggtttaaatt
     5041 aggagccaaa gacgtgtttt taaacattgc aggaggtatc aaggtggatg acccagcgtt
     5101 ggatttggca gttgttgctt cggttttatc atccaatgcc gacattgcta tcaacaaggg
     5161 aatttgttta tcggccgaag tgggattgtc tggtgaagtg cgaccagtaa accgcattga
     5221 tcaacgcatc tacgaagccg aaaaattagg ttttgaaaaa attatcattt ccaaatacaa
     5281 taaaggcatc aaacaatccg attttaaaat tgaattggtg ttgtgcagta aactcgacga
     5341 agtagtgcga gcattatttg cttgatgggg ggtaaacaat aaaatctcta taattttctt
     5401 gatgtattgt ttcaattgag ctttgatacg cttcaataaa tgcctttggt tgttttgctt
     5461 ttgctttggg attccactta aactcatacc ctttaattac tccattttgc tcttcaacat
     5521 agtcaatttc ttgttgttga acagttctcc agaaataacg attcacaaag gattgatggt
     5581 atgaattgcg tttcattcgt tctgttatca aaaaattctc ccacaaagct cctacatcat
     5641 ttctaagctc taaaggattg aagttttgaa tcaaagcatt ccttactcca ttatcgtaaa
     5701 aatagatttt tttgtttgtc tttatttcat ttcttaagtt tctacttaat gaaggaagtg
     5761 taaaaattac atacgataat tccaatagat gtatataatt tgcaactgtg tttttatcta
     5821 cgccaacaag ttgtgcaact tcattataac ttacttcact tcccacttga aaggctaaag
     5881 ctcttagaat tttctccaat acatctggtt tttgcacgct tcctaacgct aaaatatctt
     5941 tgtataaata actttgtgtt agctcattca aaagcaccac ttcctcacct ggatgattta
     6001 taatttctgg atacattcca aaaaccaatc tattttttaa attactggca gcatcaataa
     6061 aactcgttga ttgctctaat tcctcataag aaattggcat taaaaaatat tcaaattttc
     6121 ttccagttaa agcttcattt gttttgtttt ttaattcaaa ggctgacgaa ccgcttaata
     6181 taagttgcac gttttgcatt tgatcatgaa taattttagc tgtgatgcct atgttttcaa
     6241 gacgttgagc ttcatctata aatacaattt ttgagttgcc gatgatgttt tctaattctt
     6301 tagtactggg atttctaagt atagaccgtg tataagggtc atctccattc aaaaaaagat
     6361 agtctttccc gcttaacact gttttaatta atgttgtttt tccaacttga cgcgctccaa
     6421 caataacaat tgtcttgcct ttaaacaact tgttttgaat attttttact aaagttctat
     6481 taatcataac cctctatttt caccaaatat acataaaatc ggtgaattga ttcaccaaaa
     6541 atacatataa tcggtgaatt gattcaccaa aaacacctat aattggtgaa tgaaggaatt
     6601 ttttgacata aaaaaagtcc atttgctggt aatttgcaaa cggactttta gctaaaatca
     6661 atctatttat ttcttcggta aatttccaaa attagcttga atggattgga tgcttccgcc
     6721 agatacactg atggtaaaat ttccactcat tttattatcg ctaaatgctg tgatagcaac
     6781 agatgcactt ccagaattgg tataggttgt gttgtttttc atgaatgtaa aaccataaga
     6841 agagctaact gttttggtgc caatagtagt attgtcttga ttatcccaat tgatttcaaa
     6901 aaagttgctg tttccacctt tataggcacg aattcctgtt ccccaactcc ctgtggtcca
     6961 aaaagcactg tcggcaacaa tttcacttcc gccatcttct ttccaacgaa aaccatcttg
     7021 agcaactggc gcatattggc aactgccatc atctttggtt gcttctgcat taaaattggt
     7081 tgctgtgttg tccatacaac cttctttttt acacgctgta actaccaata atcccattac
     7141 aactgccgat aaaagtgtct ttttcataca attagttttt ataattgaaa acaaagatag
     7201 agcatttatc aataaaaaaa catacgtatt ttgacgtaaa aaaaggttgc caaattctga
     7261 caacctttta ctatagtgat tgtgactagt cctaaataac cgatacagat tattaagaca
     7321 aaaataatta atta
//
LOCUS       DAOQVQ010000068         7313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11200_length_7313_cov_3.212455, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000068 DAOQVQ010000000
VERSION     DAOQVQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11200_length_7313_cov_3.212455"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKN22_03400"
     CDS             complement(<1..1101)
                     /locus_tag="PKN22_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNS41781.1"
                     /translation="MKDLLEKFENKQPEIVFEWCDSETDAKGWVVINSLRGGAAGGGT
                     RMRVGLDKREVESLAKTMEVKFTVAGPAIGGAKSGINFDPKDPRKKDVLRRWYKAVSP
                     LLKTYYGTGGDMNVDEVHEVIPMCKEVGVLHPQEGVFTGHFSPSDAQKTKRIQQLSEG
                     VTMTVTDANYSPDLSKKYNVSDMITGYGVAESVKHYYEIWGGSVKDKKIIVQGWGNVA
                     SAAGFFLAQQGAKIVGIIDRVGGLIKEEGFTFEEVKHLYLTKNGNELAHPKMLSYEEV
                     NAKIWDVAADAFLPCAASRIVQQDQVERMVANGLGLIAAGANVPFADKEIFFGPIADY
                     TDNKIAVVPDFISNCGMARVFAYLMSNDIDAMT"
     gene            1283..1909
                     /locus_tag="PKN22_03405"
     CDS             1283..1909
                     /locus_tag="PKN22_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41782.1"
                     /translation="MKNILLASAIVLASFQLKAQQTSLSNPVIPERKIHTFYVEAFGQ
                     SLYYSFNYDRLLFTKKRFPGTFSVGLSVLPTPDYTQISVPFSFNGLIGKGSHHLELGI
                     GVTPMYFRERMKSIIGNSEAKQMFANDFFFYLTPKIGYRYQNPKGGFFAKATLTPPIG
                     IFSHWGGSFERNTSPTYPGHNSFFRTPVFDSNIIYPWAGISIGWTFKK"
     gene            1979..2968
                     /locus_tag="PKN22_03410"
     CDS             1979..2968
                     /locus_tag="PKN22_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNS41783.1"
                     /translation="MNTFGRLFRIHIFGESHGESVGVIIDGCPAGLAINHQALSTDLD
                     RRKGGKKGTTPRQEADTPLFKSGIFNGTTTGAPITILFENNNTRSEDYEKQRAIPRPG
                     HADFVAHQKYGGFEDYRGGGHFSARLTTGIVAAGAIAKQLLKDVSIEATITEIAGEKD
                     VEKGLEKAIAQKDTVGGIIECCVKNLPIGLGEPFFDSVESQLAHALFAIPAVKGVEFG
                     NGFEAARLFGSQNNDTIINEQGKTATNNAGGINGGITNGNDLVFRIAIKPASSTPKEQ
                     ESWNWETQQMEQFSVKGRHDLCVALRAPVIVEAMTAIVLCDFLLLEQKIGKVL"
     gene            3052..3585
                     /gene="msrA"
                     /locus_tag="PKN22_03415"
     CDS             3052..3585
                     /gene="msrA"
                     /locus_tag="PKN22_03415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNS41784.1"
                     /translation="MTTTNVDTATLGAGCFWCIEACFKDVKGILSVTPGYAGGHLKNP
                     TYKQVCEGNTGHAEVAQIVFDTTLLSYDNLLEMFWFVHDPTQVNRQGNDIGTQYRSVI
                     FYHSEAQKEIAASYIQKLTDEKVWDLPIATQLLPIDVFYPAEDYHKNYFELNPQNQYC
                     QSVVRPKVEKFKKVFQK"
     gene            complement(3726..4937)
                     /gene="sucC"
                     /locus_tag="PKN22_03420"
     CDS             complement(3726..4937)
                     /gene="sucC"
                     /locus_tag="PKN22_03420"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNS41785.1"
                     /translation="MNLHEYQGKSILKSYGVAIQEGIVIDKVEDALEAAKKLTEQTGT
                     GWYVVKAQIHAGGRGKGKIQETDSNGVVLAKGLGEVEEKVKGILGGHLMTAQTNGKAK
                     KVNKVMIAQDVYYPGEAQTEEFYMSVLLDREKGRNTIVYSTEGGMDIEAVAEHTPEKI
                     FKEVIDPRVGLQGFQARKIAFNLGVSGQAFKEMVKFVTALYNAYVGIDASMFEINPVL
                     KTSDNKVIAVDAKVTLDGNALYRHPEYAAMRDLTEEDPTEVEAGEAGLNFVKLDGNVG
                     CMVNGAGLAMATMDIIKLSGGNPANFLDVGGTANAERVEKAFHIILKDPNVKAILINI
                     FGGIVRCDRVAQGVVDAYKNMGNIPVPIIVRLQGTNAEEAKKLIDESGLNVHSAVLLQ
                     EAADKVKAVLA"
     gene            complement(5043..6527)
                     /locus_tag="PKN22_03425"
     CDS             complement(5043..6527)
                     /locus_tag="PKN22_03425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNS41786.1"
                     /translation="MKKVLIANRGEIARRVIRTLKSMDIKSVAVYSDADKNAPHVIEA
                     DEAVYLGGSPAAESYLKMDLIIDYCKQLGVDGIHPGYGFLSENAEFARKLKQNGITLI
                     GPSPESMEVMGDKLSAKQAVKGYGVPLVPGVDEAITDVEKAIQIADEIGYPILIKASA
                     GGGGKGMRLVEKSEDFREQMRLAQSEARNSFGNDAVFIEKFVTKPRHIEIQVFADRHG
                     NAVYLFERECSVQRRYQKVIEEAPSAVLTPELRKKMGEAAVAVCKACNYEGAGTVEFM
                     LDGNMDFFFLEMNTRLQVEHPVTEEITGLDLVEWQVRVARGEELPLKQEELKINGHAI
                     EVRVYAEDTLNGFTPDIGKLKRYRVPGGRAVRVDDAFVEGMDIPIFYDPMIAKLCVWG
                     KDRKSAISRAIKAIDEYQISGVKTSLDFGKFVLKHEAFRSGDFDTNFVKKYFSDPQVV
                     NTAMENEKEALKHGIDQIWNDVLKQHQKEFQSRNVESSWVKSQA"
     gene            6660..>7313
                     /locus_tag="PKN22_03430"
     CDS             6660..>7313
                     /locus_tag="PKN22_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNS41787.1"
                     /translation="MTRKKAPIDKVVAPIQKFIQQEKSGGLVLGLSVIIALFLANSPW
                     SQHYFELLEYKFGFYFNGKTYLEHDVLHWINDGLMSIFFFVVGLELKREFVAGELANP
                     RKALLPIIAAIGGMIVPAIIYLAFNPVGEVHSGWGIPMATDIAFALGVLYLLGDKIPM
                     PLKVFLTALAIVDDLGAVLVIALFYTSDISLFNLSVGLAILLVMYIGKKAGIRSVWFY
                     "
BASE COUNT         2157 a         1563 c         1223 g         2370 t
ORIGIN      
        1 ggtcattgca tcaatatcat tcgacattaa ataggcaaac acgcgcgcca ttccacagtt
       61 ggaaataaaa tctggaacta ccgcaatttt attatcggta taatcggcaa ttggtccaaa
      121 gaaaatttct ttatctgcaa aaggaacgtt ggctccagct gcaattaatc ccaaaccatt
      181 tgcaaccatg cgctccactt gatcttgttg cacaatgcgc gatgctgcac acggtaaaaa
      241 ggcatctgct gccacatccc aaatttttgc gttcacttct tcgtaagaga gcatttttgg
      301 atgcgccaat tcattgccat ttttggttaa atacaaatgt ttcacttctt caaaagtaaa
      361 tccttcttct ttaatcaaac cacctactct atcaataatt ccgacaattt tagctccctg
      421 ttgtgccaag aaaaaacctg ctgcagaggc aacatttccc caaccttgaa caatgatttt
      481 tttgtctttt acacttcctc cccaaatttc ataataatgt ttcaccgatt ctgctacgcc
      541 ataccccgta atcatatccg aaacgttgta ttttttactc aaatctggcg aataattagc
      601 atccgtcacg gtcatcgtta caccttctga caattgttga atacgttttg ttttttgggc
      661 atccgatggt gaaaaatggc ctgtaaaaac accttcttgc ggatgcaaca cacccacttc
      721 tttacacatc gggatgactt cgtgtacctc gtccacgttc atatcaccac ctgtaccgta
      781 ataagttttc aataacggtg aaacggcttt gtaccaacgg cgcaacacat cctttttgcg
      841 tggatcttta gggtcaaaat taattcccga tttagcgcca ccaatggcag ggccagcaac
      901 tgtgaatttc acctccattg tttttgccaa cgattccact tcgcgtttat ccaaacctac
      961 gcgcatacgt gttccgccac ctgctgctcc ccctctcaac gagttaatca ctacccatcc
     1021 ttttgcatcg gtttcagaat cacaccattc aaaaactatt tctggctgtt tattttcaaa
     1081 tttttctaat agatctttca ttctgtaaat tttcaaaaca gctaaactaa caattttaga
     1141 ccgaacaagc aatcttgttg tgaaatattc acagaatttg gcagtatttt cacaaataaa
     1201 gacgttctgt tcattttaag aaattttggg aagtagctat ttagttaatc gttaattttg
     1261 agtagaatat aaacctataa ttatgaaaaa catccttctt gctagcgcca ttgtattggc
     1321 aagttttcaa ctaaaagcac agcaaacaag cctaagtaat cctgttattc ctgaaagaaa
     1381 aatacataca ttttatgtag aagcttttgg acaatcgctt tattattctt ttaactacga
     1441 tcgtttgttg tttaccaaaa aacgttttcc gggcaccttt tctgtcgggt tgagtgtttt
     1501 gcccactccc gattacaccc aaatttctgt gccgttttcg tttaatggat tgattggaaa
     1561 aggcagtcac caccttgaac ttggaattgg tgttactcca atgtatttta gagaacgcat
     1621 gaaaagtatt attggcaata gtgaggcaaa acaaatgttt gcaaatgatt tcttttttta
     1681 cttaactcct aaaattggct accgttatca aaatccaaaa ggaggtttct ttgccaaagc
     1741 aacgttaact ccgccaattg gaatttttag tcattgggga ggaagttttg aacgcaatac
     1801 cagcccaact tatcctggac ataattcttt ttttagaact cctgtttttg attccaatat
     1861 catttatcca tgggctggta ttagtattgg atggactttt aaaaaataat ctacttttta
     1921 aggtctaaaa aacatgtttt gccatccaat ttagtaattt tgaatacaat ttcaaagtat
     1981 gaatacgttt ggtcgtttat ttagaattca catttttggc gagtcgcacg gtgaatcggt
     2041 gggtgtaata attgatggtt gtccggccgg acttgcaatc aaccaccaag cgctttcaac
     2101 cgatttggac cgtaggaaag gtggaaaaaa aggcactact cctcgtcaag aagcagacac
     2161 acctctattt aaaagtggaa tttttaacgg cacaacaact ggtgctccca tcactatttt
     2221 atttgaaaac aacaatacgc gttcagaaga ctatgaaaaa caacgggcca ttccacgacc
     2281 tgggcatgcg gattttgtag cccatcaaaa atatggaggt tttgaagatt atagaggagg
     2341 cggacatttt agtgcgcggt taacaactgg gattgttgct gctggagcaa ttgccaaaca
     2401 attactcaaa gacgtttcca ttgaagctac aattacagag attgcgggtg aaaaagatgt
     2461 ggaaaaagga ttggaaaaag ccatagccca aaaagatacc gttgggggta ttattgaatg
     2521 ttgcgtaaaa aacttaccca taggattggg cgagcctttt tttgattcgg tagaatcgca
     2581 attggcacac gcactttttg ccattccagc agtaaaaggt gttgaatttg gaaatggttt
     2641 tgaagcagca cgtttatttg gttctcaaaa caacgatacc atcatcaacg aacaaggaaa
     2701 aacagctacc aataatgcag gtggaatcaa cggtggcatc accaacggca atgatttggt
     2761 gtttagaatt gctataaaac cagcttcgtc aacgccaaaa gaacaagaaa gttggaactg
     2821 ggaaactcaa caaatggaac aattttcggt taaaggtcgc catgatttat gcgttgcctt
     2881 gcgtgcccct gttattgtag aggcaatgac ggcaattgtg ttgtgcgatt ttttactttt
     2941 agaacaaaaa attggcaaag tgctatgaaa aacagtgtgt ttgcgctact tttgctagcg
     3001 ttattttcaa cgtgtacagc acaaactaat tcaacaaaaa aaacacaacc catgacaaca
     3061 acaaatgtag atacagcaac gcttggtgca ggatgttttt ggtgcattga agcttgtttt
     3121 aaagatgtaa aaggtattct ttcagtaacg cctggctatg ctggtggtca tttaaaaaac
     3181 ccaacctaca aacaagtatg cgagggcaac accggccatg cagaagttgc tcaaattgtg
     3241 tttgacacta ccttattgag ttacgacaac ttactagaaa tgttttggtt tgtgcacgat
     3301 ccaacacaag ttaaccgtca aggaaacgac attggcacac aataccgttc ggtgattttc
     3361 taccattcag aagcacaaaa agaaatagca gcctcctaca tccaaaaact tactgatgaa
     3421 aaagtttggg atttgcctat tgctacgcag ttattgccta tagacgtgtt ttatccagca
     3481 gaagattacc ataaaaacta ctttgaatta aatccgcaaa atcaatattg ccaaagcgta
     3541 gttcgaccaa aagttgagaa atttaaaaaa gtttttcaaa aataaagcaa tatgaatcaa
     3601 tagtgaaaat ctgaatgaca atcagttaaa tatttgattc aaaaattaaa gcaacatttt
     3661 aatttataca acaaaaaaat ggcgttacat tgctgcaacg ccatttctaa catttaaaaa
     3721 tatatttaag ctaaaactgc tttcacttta tctgctgctt cttgcaacaa tactgctgaa
     3781 tgaacattca atcctgattc atcaatcaat tttttagctt cttcagcatt tgttccttgc
     3841 aaacgaacaa tgattggaac tggaatattc cccatgtttt tgtaagcatc aacaacacct
     3901 tgcgctactc tatcgcaacg aacaatacca ccaaagatgt taatcaaaat tgcttttacg
     3961 tttggatctt tcaaaatgat gtggaatgct ttttcaacac gctcagcgtt ggcagtacct
     4021 cctacgtcca agaagtttgc agggttacca ccagacaatt taataatatc catagttgcc
     4081 attgccaaac cagctccgtt taccatacac cctacgtttc cgtccaattt cacgaagttc
     4141 aaaccagctt cacctgcttc aacttctgtt ggatcttctt ctgtaaggtc tctcattgct
     4201 gcatattctg ggtgacggta caatgcgttt ccatccaaag taacttttgc gtcaacagca
     4261 atcactttgt tgtctgaagt ttttaacaca gggttaattt caaacataga tgcatcaata
     4321 cctacataag cattgtaaag agcagtaaca aatttcacca tttctttaaa tgcttgtccg
     4381 ctaactccta agttaaatgc aatttttctt gcttgaaaac cttgaagtcc aacacgtgga
     4441 tcaattactt ctttaaagat tttttctggt gtatgttcag caactgcctc aatgtccatc
     4501 cctccttctg tagagtaaac aatggtattt ctaccttttt ctctgtccaa aagaactgac
     4561 atgtaaaact cttctgtttg tgcttctcct ggatagtaaa cgtcttgtgc aatcatcact
     4621 ttgtttactt tcttcgcttt accgttggtt tgagccgtca tcaagtgacc tcccaaaata
     4681 ccttttactt tttcttcaac ttctccaagt ccttttgcta aaacaacccc gtttgagtct
     4741 gtttcttgga tttttccttt accacgacca cctgcatgga tttgagcttt cactacgtac
     4801 caacctgtac ctgtttgctc tgttaatttt tttgctgctt ctaaagcatc ttcaactttg
     4861 tcgataacaa taccttcttg gatagctact ccatagctct taagtattga tttcccttga
     4921 tattcgtgta aattcatgcg gaattatgtt ttttattctt ttttattgcg ttgtaaaagt
     4981 agtattttat tatgaaaagt caaataagaa actacttttt gttatttatc gttcaattaa
     5041 acttatgctt gtgattttac ccagctactt tcaacgttgc gcgattggaa ttctttttgg
     5101 tgttgtttca aaacatcgtt ccaaatttga tctattccat gtttcaacgc ttctttttcg
     5161 ttttccattg ctgtattcac cacttgaggg tctgaaaaat attttttcac aaaattggta
     5221 tcaaagtcac cacttctaaa tgcttcgtgc ttcaatacaa actttccaaa atccaacgac
     5281 gtttttacac ccgaaatttg gtattcgtca attgctttaa ttgctcttga aatagccgat
     5341 tttctatcct tgccccaaac acacaatttg gcaatcattg gatcgtaaaa aattggaata
     5401 tccatgcctt ccacaaaagc atcgtcaaca cgcaccgcac gtccacctgg aacacgataa
     5461 cgtttcaact taccaatatc tggagtaaat ccgtttaacg tatcttcagc atacacacgc
     5521 acttcaatag catggccgtt tattttcaat tcctcttgtt taagtggcaa ctcctctcct
     5581 cttgcaacac gcacttgcca ttccaccaaa tccaaaccag taatttcttc ggttacagga
     5641 tgctcaactt gcaaacgagt gttcatctcc aagaaaaaga aatccatgtt tccatccaac
     5701 ataaattcta ccgttcctgc tccttcataa ttacaagcct tgcacactgc aactgctgct
     5761 tcacccattt ttttacgcaa ttctggagtc aacacagcac ttggcgcttc ttcaattact
     5821 ttttgataac gacgttgaac cgaacactca cgctcaaaca aatatacggc attaccatga
     5881 cgatcggcaa acacttgaat ttcaatgtga cgaggcttgg ttacaaactt ttcaataaac
     5941 acggcatcgt ttccaaatga attgcgtgcc tcactttggg ccaaacgcat ttgctcacga
     6001 aaatcttcgc ttttttcaac caaacgcatc ccttttccac ctcctccggc agatgcttta
     6061 attaatattg ggtaaccaat ttcatcggca atttgaatgg ctttttctac atccgtaatg
     6121 gcttcatcaa caccaggaac aagtggaacg ccataacctt ttacagcttg tttggcactc
     6181 aatttatcgc ccatcacctc catcgattcg ggtgaaggac caattaacgt aattccattt
     6241 tgcttcaatt tacgagcaaa ttcagcgttt tctgataaaa atccataacc cggatgaatt
     6301 ccatccactc ccaattgttt gcaataatca atgatcaaat ccattttcaa atagctttct
     6361 gcagctggac ttcctcccaa ataaactgct tcgtcagctt ctattacgtg tggcgcattt
     6421 ttatccgcat ccgaataaac cgcaacactc ttgatgtcca tagattttaa tgtacgaata
     6481 actcttcttg caatctctcc tctattggca attaatactt ttttcatgga tttttctagt
     6541 ttgatacgct tgcgaaatta atattttcaa tttaatttta acgttattta atgactattt
     6601 cacatcaaaa actagttttt caaatatatt tgtaaattac acaaaatttt aactgcaaaa
     6661 tgacacgaaa aaaagcgcca attgataaag tagttgcacc cattcaaaaa ttcattcaac
     6721 aagaaaaatc aggaggttta gttctcggac taagtgtaat catcgctctt ttcttagcta
     6781 attcaccgtg gtcgcagcat tattttgaat tgttagaata caaatttggc ttttatttta
     6841 acggaaaaac ttatttagaa cacgatgtat tgcattggat caacgacggt ttaatgtcta
     6901 tcttcttttt tgtagttggt ttagaactga aacgcgaatt tgttgctggc gagttggcta
     6961 atccacgcaa agctttgctt cccatcattg ctgcaattgg cggaatgata gtgcctgcca
     7021 ttatttatct tgcattcaat cctgttggcg aagtgcacag cggttgggga attccgatgg
     7081 ctacagatat tgcttttgcc ttgggagtac tttatttatt aggcgataaa attcccatgc
     7141 ctctaaaagt gtttttaacc gctctggcta ttgttgacga tttaggagct gtgttggtaa
     7201 ttgctttgtt ttacacttcc gatatttcgt tgttcaatct ctcggttggt ttagctatct
     7261 tattggtgat gtatatcggt aaaaaagcag gaatcagaag cgtttggttt tat
//
LOCUS       DAOQVQ010000069         7292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11244_length_7292_cov_3.400028, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000069 DAOQVQ010000000
VERSION     DAOQVQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11244_length_7292_cov_3.400028"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKN22_03435"
     CDS             <1..661
                     /locus_tag="PKN22_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNS41788.1"
                     /translation="ESYKILPEAKDYKRIGEGDTGLNTGGMGAISPVPFADAAFMDKV
                     ENQIIIPTIKGLKQDNIPYKGFIFFGLINVKNEPYVIEYNCRMGDPETEVVMPRLKSD
                     ILDLFEGVATNTLSERDVQFDDRSCCTVMMVSGGYPEAYEKGKQIFGLNSVSNSIVFH
                     AGTASDGPAVMTAGGRVLAVSSYGRTIENALDKSYDSIKNIEFEKAYFRKDIGFDVVE
                     R"
     gene            complement(816..1481)
                     /locus_tag="PKN22_03440"
     CDS             complement(816..1481)
                     /locus_tag="PKN22_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41789.1"
                     /translation="MNKFFNALLFVVFIPTLLLSIFVGFDIPIPLLRTTGAYLTYNNE
                     ALFVLGLFILLINVRRSVKRWTALSLVNKVHKFKWTTLVTKERVKRVYVYNFSEALVM
                     LMAGLCLYYICADAWMPMIGFLFGALDAVVFAIIGASSNKFRVGMTSKALVAGDRDVI
                     VIYFTGLRKVSTHLQTIFFDFKDDLQLRFPMELIPEEKRTEFFQELKNTVNPDKVYFQ
                     NDL"
     gene            complement(1474..3183)
                     /locus_tag="PKN22_03445"
     CDS             complement(1474..3183)
                     /locus_tag="PKN22_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNS41790.1"
                     /translation="MESTAFLEELKALTAQEDVISTGREVNELSARFEDYVLEEERKL
                     QIAQMEAHENDAEIPDNTELRQLKHDFYEELKAYREKRKTLIDAKNAVEHTNLSKKKA
                     LINKLRDIIQNEEKIGAAFSALKEIQETWKEIGDIPRDVRHDIQSEYSRLVEDFFYNI
                     NIYKELKEHDLKRNLQLKQEVIEKLKQLQNLTSIKEIEADLKALQNEWEDVGPVNNND
                     WELLKDEYWKNVHACYSKINAHYEDRRAALMKNLEQKQAILKQITERIDQIPASTDVK
                     FWNHETKEILNFQEEWKKVGFGPRKENDEIWTAFRAQCDRFFNLKKEFYSNIQDKFDV
                     IAEKKKEIIAKANELKDSTDWKKTSMQLINLQNRWKELGNAGMRHEQKLWKQFRHACD
                     VFFDNKQKFFSEADAEFESNLAAKNAVIEKIKAYTMPEDKKQVIADLKQFTNEFNTIG
                     KVPMKVKDAVYNAYKAALDVHYNALKLEGEEKNKIMFQARIDTLAGSPDAARLFAREK
                     ADIRKKIDLLNHDILQLENNLGFFANSKGADTLKKEVEKKIERAKNEILALRQRIKLI
                     PNE"
     gene            complement(3199..4125)
                     /locus_tag="PKN22_03450"
     CDS             complement(3199..4125)
                     /locus_tag="PKN22_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS41791.1"
                     /translation="MSLLTVGSVAFDAIETPFGKTDKIIGGAATYIALTASFFEEDQK
                     IVSVVGEDFPQEMIDLIASKGVDVTGLQIKKGEKSFFWSGKYHNDMNSRDTLVTELNV
                     LGDFDPVIPDSYQGVEYLLLGNLSPQVQRQVIERLHQRPKLIAMDTMNFWMDIAMDDL
                     RQTLKLVDVLIINDEEARQLSKEYSLVKASKVIREMGPKYLIIKKGEHGALLFHGDNV
                     FFAPALPLEEVFDPTGAGDTFAGGFMGYIASTDDSSFENMKRGIIAGSALASFCVEKF
                     GTERLQEISKEEINQRIEKFVQLANFDMLQTI"
     gene            4426..>7292
                     /locus_tag="PKN22_03455"
     CDS             4426..>7292
                     /locus_tag="PKN22_03455"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNS41792.1"
                     /translation="MTRILQTAFYVLALSFSLFSQPINYTTPGTYTWTVPPCVYEITV
                     KVWGGGGGGGGALAIMKNSSSGGETCSGGAGGGGGGFTSKTFSVTPGQTYTVVVGAGG
                     TAGIAGAGSWNGGITTPAGNGGTGGTSSFTGNGQNLNATGGIGGGGASGYNTTTSAVD
                     LNTVGTGGAGGVGSGGSSNFTGGAGGSGYIPGNFSNDFSGPGGGAAGPGGNGGSNTPP
                     YAGVADPAAGVGQAPGGNGAKGRYNNQPGKQEKAGYNGLTYGGGGGGAVAHIDPYNVA
                     KAAGGAGANGAVIIEYTSSGSPTPTPVITLTAATCSADGTVKISNYSASNSYSFTPSG
                     PTVGAGGVISGMVAGTTYTVTATASGSCESSASSSFSLSPQLTPPTVTVSPNATICSG
                     NSATLTAGGADTYVWSPTTNLSSSTGSSVIASPTTTTTYTVTGTASNGCTNSAQVTIT
                     VTPPPTANAGNDVTTPCGIGVQIGETTSTTVSPMAPAGCSGASQMIVNPAGGAYINSV
                     STTGGLTTNISNLNTLVDGNNPQTDYIAFNRWYSNFTNHLVDANQGGSFQLNLQGNKG
                     ATASNPPTFSFRVWIDWNNDGNFDNIPGSELVHTTATTASNPFNFNNITINVPSNAAI
                     ATVRMRIRVKYGAEFVASDASCTKSNPNGWPGQGNYDHTSEVEDYSVQIYPAGGGGGN
                     SNYTYSWTPTTGLDDPTISNPTTTPSSTTTYYVTVTDTQTGCTATDSVVVTVNQPSPI
                     FNPIAPFCAGTTAPTLPTTSTNGIAGTWNPAVVSNTNSGSYTFTPNVGTCASDTTIQI
                     VVNPATETPIISTTPATCTDQGSSSITNYSAGNTYTFSPTGPTIAANGAISGMTIGTA
                     YTLIASNGSCPSNPSNPFTNDDVLSAATSPTVATTAASCLSAGTASINNYSASNNYVF
                     TPPGPTVGLDGVINNMTYDQNYTVAIDNGGCSSGESDPFSI"
BASE COUNT         2147 a         1459 c         1332 g         2354 t
ORIGIN      
        1 tgaatcctat aaaattttac cagaagcaaa agattacaaa cgcattggtg aaggcgatac
       61 aggtttgaat accggaggaa tgggagctat ctcacccgtt ccatttgcag atgctgcttt
      121 tatggataaa gttgagaatc aaattattat tccaaccatt aaaggtttaa aacaagacaa
      181 catcccctac aaaggcttca tattttttgg gttgattaat gtgaaaaacg aaccttacgt
      241 tattgaatac aactgccgaa tgggcgaccc agaaacagaa gtggtaatgc cacgtttaaa
      301 atccgatatt ttggatttgt ttgaaggtgt agcaaccaac acattgtctg aacgcgacgt
      361 tcaatttgac gatagaagtt gttgcacagt aatgatggtt tctggcggat acccagaagc
      421 atatgaaaaa ggcaaacaaa tctttggttt gaacagcgta tcaaactcca tcgttttcca
      481 tgctggaact gcctctgatg gtcctgcggt aatgacagca ggtggtcgtg tattagctgt
      541 ttcttcgtat ggacgcacca ttgagaatgc attggataaa tcctacgata gcatcaaaaa
      601 catcgaattc gaaaaagcat atttcagaaa agacatcggt tttgatgttg ttgaaagata
      661 aagaaacaag tctttaagaa aatcgtttca accgatctta ttgagtcgta ttaatttcta
      721 cattaaatgt aggaatactt ctgtcctatt tgcctattca aagcagtgaa aagaagaatt
      781 aaattataca aaacggtttt ctttcgaatg attttttaca aatcgttttg aaaatatact
      841 ttgtctggat taaccgtatt ttttagctcc tgaaagaatt cggtgcgctt ttcttcagga
      901 attaattcca ttgggaatct caattgcaaa tcatctttga aatcaaaaaa tatggtttgt
      961 aaatgcgtag aaacttttct taacccagta aaataaatta caatcacatc tctgtcgcct
     1021 gcaaccagtg ctttggaagt cattcctact ctaaatttgt tacttgaagc tccaataata
     1081 gcaaatacaa cagcatccaa cgctccaaag agaaaaccaa tcataggcat ccacgcatca
     1141 gcacaaatgt aatacaaaca caaacctgcc attagcatca ccaatgcttc agagaaattg
     1201 tacacatata cacgttttac acgctctttg gtaacaagcg ttgtccattt gaatttgtgc
     1261 accttgttaa caagactgag tgctgtccaa cgttttacac ttctgcgcac attgatgagc
     1321 aaaatgaaca atcccaacac aaacaaagcc tcgttgttgt atgttaaata tgcacctgtt
     1381 gttcttaaaa gcggaattgg aatatcaaaa ccaacaaaga tggacaacaa taaggttgga
     1441 ataaacacta caaaaagcag tgcattaaaa aatttattca tttggaatca attttatgcg
     1501 ttgacgcagt gccaagattt cgtttttagc acgttcaatt ttcttctcca cttctttttt
     1561 caaagtatcg gcacctttag aatttgcaaa aaatccaagg ttattttcca attgcaatat
     1621 atcgtggttc aataaatcaa tttttttacg aatatctgct ttttcacggg caaatagacg
     1681 tgctgcatct ggacttccag ccaaggtatc aatgcgtgct tggaacatga ttttgttctt
     1741 ttcctctcct tccaatttta atgcattgta atgcacatcc aacgctgctt tgtaggcatt
     1801 gtaaacggca tcttttactt tcattggcac cttcccgatg gtgttaaatt cattggtaaa
     1861 ttgctttaag tcggcaataa cctgtttttt atcctcgggc atggtataag ccttgatttt
     1921 ttcaataaca gcatttttag cagctaaatt gctttcaaat tcagcatctg cttcgctaaa
     1981 gaatttttgc ttgttgtcaa aaaacacatc gcatgcatgg cggaattgtt tccataattt
     2041 ttgctcgtga cgcatacctg cgttgcccaa ttccttccaa cggttttgta agttaatcaa
     2101 ctgcatcgaa gtttttttcc aatcggttga atcttttaat tcgttggctt tagcaataat
     2161 ttctttcttc ttctcagcaa tcacatcaaa tttatcttga atgttgctgt aaaattcttt
     2221 tttcaagttg aaaaaacgat cgcattgagc tctaaaagca gtccaaattt catcgttttc
     2281 tttgcgcgga ccaaaaccaa cttttttcca ttcttcttgg aagtttaaaa tttctttggt
     2341 ttcatggttc caaaatttaa cgtcggttga tgctggaatt tggtctattc tttcagttat
     2401 ttgttttaaa attgcttgtt tttgctccaa atttttcatc aatgccgctc ttctatcttc
     2461 atagtgtgcg ttaatcttgc tgtaacaagc atgtacgttt ttccaatatt catctttcaa
     2521 caattcccaa tcgttattgt ttaccggacc cacatcttcc cattcatttt gaagcgcttt
     2581 caaatctgct tcaatttctt tgatagaagt taaattttgc aactgtttca atttttcaat
     2641 cacctcttgc ttcaattgca agttgcgttt caaatcgtgc tcttttaatt ctttgtaaat
     2701 attgatattg taaaagaaat cttctactaa gcgagagtat tcggactgaa tatcgtgacg
     2761 tacatcgcgt ggaatatcgc caatttcttt ccaagtttct tgaatttctt ttaatgctga
     2821 gaatgcagca cctatttttt cttcgttttg aataatatcg cgtaacttgt tgatgagtgc
     2881 ttttttcttt gaaagattgg tatgctcaac cgcgttttta gcatcaatta acgttttgcg
     2941 tttttcacga taggctttca attcttcata aaaatcgtgc ttcaactggc gcaattccgt
     3001 attatctgga atctcagcgt cattctcatg cgcctccatt tgagcaattt gcaacttacg
     3061 ttcttcttct aaaacataat cctcaaaacg agcactcaac tcgttcactt ctcgtccagt
     3121 tgaaattaca tcttcttgcg ctgtcaacgc tttcaactct tctaaaaatg cagtactttc
     3181 catattattc atttactatt aaatggtttg caacatgtca aaattggcaa gttgcacaaa
     3241 tttttctatt ctttgattga tttcttcttt tgaaatttct tgcaaacgct ccgttccaaa
     3301 tttttcaaca cagaaggaag ccaatgccga tccagcaatg ataccacgtt tcatgttttc
     3361 aaacgaactg tcatctgttg aagcaatgta tcccataaat ccacctgcaa aggtatcacc
     3421 tgcacctgtt gggtcaaaca cttcttctaa tggcaatgcg ggagcaaaaa atacattgtc
     3481 tccgtgaaat aacaaggcgc catgttctcc tttcttgata atcaagtatt ttggtcccat
     3541 ttcacggata actttggatg cttttaccaa tgaatattct ttggataatt gacgtgcttc
     3601 ctcatcgttg ataatcaaca catctaccaa tttcaacgtt tgtcttaaat cgtccatggc
     3661 aatgtccatc caaaaattca tggtatccat agcaatcaat tttggacgtt gatgcaaacg
     3721 ctcaattacc tggcgttgaa cttgtggact taaatttcca agcaataaat actcaacacc
     3781 ttgataagag tctggaatca cggggtcaaa atcacccaat acgtttaatt ctgtaaccaa
     3841 cgtatcgcga gagttcatgt cgttgtggta ttttcctgac caaaaaaatg atttttcacc
     3901 ttttttgatt tgcaacccag taacatctac tcctttagaa gcaattaagt caatcatttc
     3961 ttgtggaaaa tcttcgccaa ctactgaaac aattttttga tcttcttcaa agaatgaagc
     4021 tgttaaagct atgtaagtag ccgctccacc aataatttta tctgtttttc caaaaggtgt
     4081 ttcgattgca tcaaaggcaa cacttccaac cgtcaataaa ctcatcttta taaaatttag
     4141 gttacaaaga taaagtattc aatcaaagtt taaccatttt tcatgagcaa tctgctttaa
     4201 ttgaagctta ttttgtgatt tttctgtttt tcattggttg tttaattatt tttttaacaa
     4261 tcttacactt atatgtctat tattcataat tttaacggaa aatagaactg tttatattta
     4321 ttacttcaat ttttaacatt ttagattaac gaatattctg tttagttata cttataaact
     4381 actctaactt ttatatttag aattattaaa ctaagtttaa ataacatgac aaggatacta
     4441 caaactgctt tttatgtttt agcattaagt ttttctttat tttctcaacc cattaattac
     4501 acaaccccgg gcacatatac atggactgtt ccaccatgtg tgtatgaaat tactgttaag
     4561 gtttggggcg gtggtggcgg cggcggcggc gctttggcca taatgaaaaa ttctagttct
     4621 ggtggcgaaa catgttctgg tggagctggt ggtggtggtg gtggtttcac ttccaaaacc
     4681 ttttctgtaa cacctggtca aacatatact gttgttgtgg gagctggcgg aacagcggga
     4741 attgctggtg cgggtagttg gaatgggggt attactactc ctgctggtaa tggtggtact
     4801 ggtggcacaa gttcatttac tggaaatggg caaaacttaa atgctactgg tggtattggt
     4861 ggtggcggag caagtggcta caatacaacg acttcagcag ttgacctaaa tacggttgga
     4921 acaggcggtg ctggtggagt tggttcaggc ggaagttcaa atttcacagg cggtgctggt
     4981 ggaagtggtt atattccagg gaactttagt aatgacttta gtgggccagg tggcggggct
     5041 gcggggccag gtggtaatgg tggctctaac acacctccct atgcgggtgt agcagaccca
     5101 gcagcaggtg ttggacaagc ccctggtggc aatggtgcta aaggaaggta taacaatcaa
     5161 ccaggaaaac aagaaaaagc aggatacaat ggtttaactt atggcggtgg cggtggcggg
     5221 gctgttgcgc atatagatcc atacaatgtt gcaaaagcag ctggaggcgc aggagcaaac
     5281 ggtgcagtaa taattgagta tacatcttca ggctctccta ctcctactcc tgttattacc
     5341 cttactgcgg caacttgctc agcagatgga acagtaaaaa tatccaacta ttcggcaagc
     5401 aattcgtata gctttacacc atctggacca actgttggtg ctggaggtgt tatatccggt
     5461 atggttgcag gaacaacata taccgtaaca gcaacagcaa gcggttcttg tgaatcttct
     5521 gcaagttcgt catttagctt aagtccacag cttacacctc ctacggttac agtttctccg
     5581 aatgctacaa tttgttcggg aaattcagca acattaactg ctggaggcgc agatacttat
     5641 gtgtggtcac ctaccacaaa cttaagttct tctactggta gttcggtaat tgctagtcca
     5701 acaactacaa ctacatacac agtaacagga actgcctcaa atggatgtac aaactctgct
     5761 caggttacca taactgttac ccctccacct acggcaaatg caggaaatga cgtaacaacg
     5821 ccttgtggaa ttggagttca aattggagaa actacttcaa caaccgtttc accgatggct
     5881 cctgctggtt gctcaggcgc ttctcaaatg atagtaaatc ctgctggtgg ggcttatatt
     5941 aactctgttt ctaccacagg tggtttaaca acaaatatta gcaatctaaa tacattagta
     6001 gatggtaata atcctcaaac cgattacatt gcttttaata gatggtatag taattttaca
     6061 aaccacctag ttgatgctaa tcaaggtgga tcatttcaat taaacttaca gggaaataaa
     6121 ggagcaacag cctctaatcc tccaactttt tcatttagag tttggataga ttggaacaac
     6181 gatggtaatt ttgataatat tcctggtagt gaattggtgc atacaacagc aaccactgct
     6241 tcaaatcctt ttaattttaa caacatcaca atcaatgttc cttctaatgc tgcaattgca
     6301 acagtaagaa tgaggatacg tgtaaaatac ggtgctgagt ttgttgcatc agatgcgtct
     6361 tgcacaaaaa gcaatcctaa tggctggcct ggacaaggca actatgatca cacttcagag
     6421 gtagaagatt attccgttca aatttatcct gctggtggag gaggtggtaa tagtaactac
     6481 acttattcat ggactccaac tacagggctt gacgatccaa caatttccaa cccaacgaca
     6541 accccaagtt caacaactac ttattacgta actgtaactg atacacaaac tggttgtaca
     6601 gcaactgact cagtggttgt tacggtaaac caaccttcgc caatatttaa tcctattgct
     6661 ccgttttgtg ctggaacaac tgctccaact ttaccgacaa catctacaaa tggtattgca
     6721 ggaacttgga atccagctgt tgttagcaac actaattctg gtagctatac atttacccct
     6781 aatgttggta catgtgcatc agacacaact atacaaattg ttgtcaaccc tgcaacagaa
     6841 acaccgataa ttagcacaac tcctgcaact tgtaccgatc aaggaagtag ttctattact
     6901 aattattctg ctggtaatac ttatacattc tcacctacag gtccaacaat tgctgctaat
     6961 ggtgcaatca gcggaatgac tattggaact gcttacacgt taattgcttc aaatggtagt
     7021 tgcccgtcta acccgagtaa tccatttact aatgatgatg tattgagtgc tgcaacttcg
     7081 ccaactgtag caacaacagc tgcatcatgt ttaagtgcgg gaacagcttc aattaataat
     7141 tatagtgcgt ctaacaatta tgttttcact cctcctggtc caacagttgg tttagatggt
     7201 gtaattaaca atatgactta tgaccaaaat tatacagtag caattgataa tggaggatgt
     7261 tcatctggtg aaagcgatcc ttttagcatt ag
//
LOCUS       DAOQVQ010000070         7279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11266_length_7279_cov_4.439922, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000070 DAOQVQ010000000
VERSION     DAOQVQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11266_length_7279_cov_4.439922"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKN22_03460"
     CDS             <1..546
                     /locus_tag="PKN22_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41793.1"
                     /translation="FNSQLDYWRLFIGMDEDISGLRWGKLHLHLEASQTFGDVPLLLK
                     QYAVGTRRNIGLVTANAMETVYPGEFYHDRQASFIVRYGTPAIKTKKSWFAPEFIFHH
                     GIAVGDFANKVQHNTTFWTMDKGVFEGGLILTGLVNYNILKLGVGVFYRYGAYSHTKV
                     VDNLVPKISITINSFQQGNNN"
     gene            complement(543..1697)
                     /locus_tag="PKN22_03465"
     CDS             complement(543..1697)
                     /locus_tag="PKN22_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNS41794.1"
                     /translation="MKVGVLGAGTMGSGITQVAAQAGHEVVLVDLNQAVLDKAKSNLE
                     KVLSKLVEKEKISAAEATQIQNRLVYSSATADFKECGIIIEAIVENIAVKHTVFKELE
                     AIVSDSCILASNTSSLPIASIGSALQNPSRIIGIHFFNPAPLMPLVEIIPAVQTAPAV
                     VEQAKQLIDSWKKVTVLCKDTPGFIVNRVARPFYGEALRIYEEGIADFATIDWALTEI
                     GGFRMGPFTLMDYIGNDINYTVTESVFAAFYYDPRFKPSFTQKRHFEAGFLGRKSGRG
                     YYNYAEGAVQPEPTKDVQLGQQIVDRVLVMLINEAIDALFLNIASKQDIDLAMTKGVN
                     YPKGLLAWAEEKTLPWVLAQLENLFEEYGEDRYRPNPLLKRLVKQGATQF"
     gene            complement(1795..2523)
                     /locus_tag="PKN22_03470"
     CDS             complement(1795..2523)
                     /locus_tag="PKN22_03470"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNS41795.1"
                     /translation="MRTIITFVFLVAFIVKSEASELYLRIASSGDYVVTVFDQVHQNK
                     SNIYKFSGLPNGINSILVVNKYTNMVLYNGTITLLPNERIVAEINTFGQFSIRQKVQI
                     QEVNWYTVLENNNYPSNPYPNNPYPNHPPHPHNPYPGAPNNSFPNNYVDDNTFNLFLQ
                     TLKDESFDSNKLKMAKNYASKSALSAQQILKVCKEFTFDSNRLDFAKSAYVTCYDKNN
                     YFLLKNAFDYSSSYNSLLEYTEKQ"
     gene            2745..5312
                     /locus_tag="PKN22_03475"
     CDS             2745..5312
                     /locus_tag="PKN22_03475"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNS41796.1"
                     /translation="MKKVAISFVATLLLSLTSNAQLTVSPYNNGSGNNGAGGNLQQFV
                     QNNLIGSGITISNVTFVGANRQMGSFNATNTVLGTVQNMNTGIILSTGMAETAIGPND
                     VPYACHAPYTAPYPSEPGGYIGNTTNDPDLSDLVGSNIKDKAILEFDFVPTGPTIEFK
                     YIFGSEEYPEFVNDFNDAFGFFLSGPGITGPYSNNSINLAIVPGTSDPVTINTVNSTV
                     NSSYYVYNGDNGSGNTVAPYKNQNQYIQYDGYTTTLTATANVQCGQTYHIKIAIADAR
                     DQAYDSGLFIKGGSLSSSTVTANDATISCSQSSVQLSVSTTVSNPQYSWSGPGIVSGA
                     NTATPTVNQPGTYTVTITSNGCVIEEDVEVTSDGSQTTPTFTNPGPICSGTSFALPTT
                     SDNGITGTWDVAPNTSQTTTYTFTPSSGQCANTVTMEVVVNSSVTPTFTNPGPVCAGT
                     SFTLPTSSNEGISGTWSPAIDNTQTTTYTFTPASGQCATTTTMQVVVSNEVTPTFTNP
                     GPVCEGTSFTLPTTSNNGITGTWDMVPNTAQTTTYTFTPTSSGCATTTTMEVVVTTPT
                     TPTFTNPGPVCAGTAFTLPTSSNEGISGTWSPAIDNSQTTTYTFTPASGQCAAATTIE
                     VVVSNEVIPTFTNPGPVCEGTSFTLPTTSNNGIIGTWDLAPNTTQTTTYTFTPTTSGC
                     ATTTTLTVAINPTTTPDFSPVGPICVGEIFVLPDTSTNGIVGTWSPAIDNTQTTNYTF
                     VPQSSGCVQNAAITVVVDPLLCAQDPIIHIPNVFTPNGDNTNETLDLLPQNIQELEYW
                     IINRWGEVMYHSTSLTDFWNGKVKGKEAPEAVYFIKYKAKGSNDKLLEGHSYFHLQR"
     gene            5339..>7279
                     /locus_tag="PKN22_03480"
     CDS             5339..>7279
                     /locus_tag="PKN22_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41797.1"
                     /translation="MNNPVHHKTHKPLLLLTFIVFIVQLSIAQAPPGMPPCGTNDAPG
                     ETVCTATPICNLNGYCGQTSTSYAADSWPELATTFQNCTSNGWDYGTIENNSFLSFVA
                     SSSNISFDTYVYNCAGLGTGGIQLMLFSADNCSSGPVTAYTCALEFQEQSTPHNLAAT
                     GLTPGNTYYIMIDGYAGKQCDYTFVATEGVAMPSVEITPGPSVNLCQGESVEVTASGG
                     SGTYTWNADPALSATTGATVTITPPTTVGAYTYVVQSSGGTTFCPENSSDTLTVNVTD
                     CGTCNVGLSLTSTDCNAANNTYDATVSIAFANQPTTGDLILTACDGSTQTIPVASLGT
                     SSYTATIPNLSLATANCQVVASFSATPDCADTLVITPSAAQTPTFTNPGPLCVGQAYT
                     LPTTSDNGITGTWAPAYNANQTTTYTFTPTGNDCATTATMTITITNEITPTFTNPGPM
                     CVGQTYTLPTTSDNGITGTWTPAYNANQTTTYTFTPTSSTCATTATMTIDVVNEIVPT
                     FDPITPICLGTDYALPTTSTNGVVGTWSPAINNTQTTTYTFTPTSSSCAVSTTLVVET
                     DETLCPEQPFIELPNVFTPNGDNVNETYILTLKGIESIEYWIVNRWGEMVYHSTSLTD
                     FWNGKTKGGSSAEGVYFIKYKAK"
BASE COUNT         2268 a         1518 c         1342 g         2151 t
ORIGIN      
        1 tttaattcac agttggatta ttggcgtttg tttatcggaa tggacgaaga tatatctgga
       61 ctccgttggg ggaaattgca cttgcattta gaagcttcgc aaacttttgg cgatgtgcct
      121 ttattgctca aacaatatgc agtaggtaca cgcagaaata ttggattggt tactgccaac
      181 gccatggaaa cagtttatcc gggtgagttt tatcatgata gacaagcctc ttttattgta
      241 cgctacggaa caccagccat aaaaacaaaa aagagttggt ttgctcctga gtttatattt
      301 caccacggta ttgcagtggg tgattttgcc aacaaagtgc agcacaacac aacgttttgg
      361 accatggaca aaggagtgtt tgaaggtggt cttatcctaa ctggattggt taactataac
      421 atcctgaaac tgggtgttgg tgtgttttac cgctatggtg cttattcgca cacaaaagtg
      481 gtggacaatt tagtgcccaa aataagcatt accatcaaca gttttcagca gggaaataac
      541 aactaaaact gagttgctcc ttgtttaacc aaacgtttta aaagtgggtt tggtcggtaa
      601 cgatcttcac cgtattcttc gaataagttt tccaattgtg ccaataccca cggcaatgtt
      661 ttttcttctg cccatgccaa caatcctttt ggatagttaa cacctttggt cattgccaaa
      721 tctatgtctt gtttggatgc aatgtttaaa aataaagcgt caattgcttc gttaatgagc
      781 atcaccaata cacggtcaac aatttgttgt cctaattgca cgtcttttgt aggttctggc
      841 tgaacagctc cttcggcata attgtaataa cctctacctg attttctacc caaaaatcca
      901 gcttcaaaat ggcgtttttg agtaaacgaa ggtttgaaac gcgggtcgta ataaaatgct
      961 gcaaaaactg attccgttac cgtataatta atatcgtttc caatgtaatc cattaatgta
     1021 aatggtccca ttctaaatcc accaatttct gttagcgccc aatcgatggt tgcaaaatcg
     1081 gcaattcctt cttcataaat acgcaatgct tctccataaa aaggacgagc aacacggttg
     1141 acaataaatc cgggcgtgtc tttgcataaa acagttactt ttttccagct gtcaattagt
     1201 tgtttagctt gttcaaccac cgctggagca gtttgaacgg ctggaataat ttctaccaac
     1261 ggcatcaagg gcgctgggtt gaaaaagtgt attcctatga tgcgagaagg gttttgaagt
     1321 gccgatccta tggatgcaat tggcaacgat gacgtgttgg aagccaaaat acatgaatca
     1381 gaaacaatgg cttctaactc tttgaatacc gtatgcttaa ctgcaatgtt ttctacaatt
     1441 gcttcaatga taattccaca ttccttaaaa tcagcagtag cagaagaata cactaaacga
     1501 ttttgaattt gagtagcttc agcagcagaa attttttctt tttctaccaa tttgctcaat
     1561 actttttcaa ggttagattt tgctttgtct aacaccgctt gattcaaatc tactaaaaca
     1621 acctcgtgtc cggcttgagc tgccacttgt gtaattccac tacccattgt tccggctcct
     1681 aatactccta ctttcattgc gacttttaat tttatgctgc acaaatgtat aaaaaaaccc
     1741 cctgaaaaaa atattccaga gggctagtgg caggtttatt ttcttgttaa atttttattg
     1801 cttttctgta tattccaaca aactgttgta actagaagaa taatcaaaag cattttttag
     1861 taaaaaataa ttgtttttat cgtaacatgt gacataggcg ctttttgcaa aatctaatcg
     1921 attgctatca aaagtaaatt ctttgcagac ttttaaaatt tgttgcgcac tcaatgcact
     1981 ttttgatgca taatttttag ccattttcaa tttattgctg tcaaatgact catctttaag
     2041 tgtttgtaaa aacaaattaa aggtattgtc gtctacataa ttgttaggaa atgaattgtt
     2101 gggcgcacct gggtatggat tgtgaggatg cggtggatga ttgggatatg gattattagg
     2161 ataaggattg cttggataat tgttgttttc aagcacggtg taccaattca cttcttgaat
     2221 ttgaactttt tgacgaatag aaaactgacc aaaagtattg atttctgcaa caattcgttc
     2281 gttgggcaaa agtgttatcg tgccgttata caaaaccatg ttggtgtatt tattgacaac
     2341 caaaatagaa ttgatgccat ttggtaaacc agaaaattta tagatgttgc ttttattttg
     2401 atgcacttga tcaaaaacgg ttacaacata atcgccagat gatgcaattc tcaaatacaa
     2461 ttctgacgct tcacttttta caataaaagc tactaaaaat acgaatgtaa tgattgttct
     2521 catagctcca aattttaaat ttaatttttc atggactaaa atggtgccaa aaaatagtgg
     2581 tttactaaaa atcattctat aaaatgtatt ctatttgatt ttgtgacagt tgtgtattca
     2641 attgtttgtt taatattttc ttgttttgtt aaatatatga ataaacatgc aacttttgtg
     2701 tttatattta agtccttaaa cttgaactag aataattaat atttatgaaa aaagtagcaa
     2761 tatctttcgt tgctactcta ctgcttagtt taacttccaa tgcacagtta actgtttcac
     2821 cctacaacaa cggttcggga aacaatggag ctggcggaaa tcttcaacaa ttcgtacaaa
     2881 acaatttaat tggatctgga attaccattt ccaacgtaac ttttgttggt gctaacaggc
     2941 aaatgggtag ttttaatgca accaatactg ttttaggaac tgttcaaaac atgaacaccg
     3001 gtattatttt atcaactgga atggcagaga cagctattgg tccaaacgat gttccctatg
     3061 cttgtcacgc accctataca gcaccttatc caagtgagcc aggaggttat atcggtaaca
     3121 caaccaatga tccagattta agtgatttag tgggttcaaa cattaaagac aaagctattt
     3181 tagagtttga ttttgtgcca actggaccaa ctatagaatt caaatatatt tttggatcgg
     3241 aagaatatcc agaatttgta aacgatttta acgacgcttt tggatttttc ttgagtggac
     3301 cgggaataac tggcccctac agcaacaatt ccatcaattt agcaatagtg ccaggcacct
     3361 ctgatccagt tactatcaat acggtaaaca gcacagtcaa ctcaagttat tacgtttata
     3421 atggcgacaa tggcagtggc aatacggttg caccctacaa aaaccaaaat caatacattc
     3481 aatacgatgg ttataccacc acattaacag caacagccaa tgtgcaatgc gggcaaacct
     3541 atcacatcaa aattgccatt gcagatgcgc gtgaccaagc gtatgattct ggattgttta
     3601 tcaaaggagg ttcgttatct tcttctaccg ttacggccaa tgatgcaacc atttcttgct
     3661 cgcaaagttc ggtgcaatta tcggtttcta caactgtttc caatccacaa tacagttggt
     3721 cgggacctgg aattgtgtct ggtgctaata cagccacgcc aacagttaac caaccgggaa
     3781 catacaccgt aaccattacc tctaatggct gtgtgattga agaagatgtt gaggttacct
     3841 ctgatggtag tcaaacaaca cctacgttta ccaacccagg accaatttgc tctggaacta
     3901 gttttgcatt accaactaca tccgataatg gaattacagg aacgtgggac gttgcaccca
     3961 acacgtctca aaccaccaca tacaccttta caccaagcag cggacaatgt gcaaacacgg
     4021 ttacaatgga agttgtagtt aattcttctg taactccaac gtttaccaat ccaggaccag
     4081 tttgtgcagg aacatcattt acacttccaa catcatccaa cgaaggaatt tcgggaacat
     4141 ggtcgcctgc aattgataac acgcaaacta ccacttatac gtttactcct gcaagtggtc
     4201 aatgtgcaac cactaccacc atgcaagtag ttgtgagcaa tgaagtaact ccaacattta
     4261 ccaaccctgg accagtttgt gaaggaacta gctttacttt accaaccacc tccaacaatg
     4321 gaattacagg aacgtgggac atggtgccca acactgcaca aaccaccact tatacgttca
     4381 cgccaacatc aagtggatgt gcaaccacca caacaatgga agtggtcgtc acaacgccca
     4441 caacaccaac gtttaccaat ccgggaccag tttgtgcagg aacagctttt acacttccaa
     4501 catcatcaaa cgaaggaatt tctggaacat ggtcgccagc aattgataat tcgcaaacca
     4561 ccacctatac atttacccca gcaagtggtc aatgtgcagc agccactacc atagaagttg
     4621 ttgtgagcaa tgaagtaatc ccaacattta ccaaccctgg accagtttgt gaaggaacta
     4681 gttttacatt gccaaccacc tccaacaatg gaattatagg aacgtgggac cttgcaccca
     4741 acacaactca aacaaccact tatacgttca cgccaacaac aagtggatgt gcaaccacca
     4801 caacattgac ggttgcaata aatcctacaa caactcccga tttttctcct gttggaccaa
     4861 tttgtgtggg tgaaatattt gttttacccg atacgtcaac aaatggaata gtaggtactt
     4921 ggtcgcctgc aattgataac acgcaaacta ccaattacac gtttgtgcct caatcttctg
     4981 gttgtgtgca aaacgcagct ataacggtgg ttgttgaccc acttttgtgt gcacaagatc
     5041 caattattca cattccaaat gtatttacac caaacggtga taacaccaac gaaacattgg
     5101 atttattgcc acaaaacatc caagaattag aatattggat tatcaaccgt tggggagaag
     5161 taatgtacca ttccaccagt ctaacagatt tttggaacgg aaaagtgaaa ggaaaagaag
     5221 cgccagaagc agtttatttt atcaaatata aagcaaaagg cagtaatgat aaactattgg
     5281 aaggtcattc atacttccat ctacaacgtt aaattgttaa actataaata aacagaaaat
     5341 gaataatcca gttcaccaca aaacacacaa acccctactc ctccttactt ttattgtgtt
     5401 tattgtgcag ctttccattg cgcaggctcc tccgggaatg cctccttgtg gaacaaatga
     5461 tgcaccgggt gaaaccgttt gtacagcaac acctatttgt aacttgaatg gttattgtgg
     5521 acaaacatct acttcatatg ctgctgactc atggccagaa ttagcaacaa cttttcaaaa
     5581 ttgtactagt aatgggtggg attatggaac catagaaaat aattcctttt tatcctttgt
     5641 agcaagttct tccaacatta gttttgatac ctatgtttac aattgtgctg gtttaggaac
     5701 aggtggaata caattgatgt tgttttctgc agacaactgt tctagtggac ctgttacagc
     5761 ctacacttgt gccttagaat ttcaagaaca aagcacacct cataacctag ctgcaactgg
     5821 attaactcca ggaaacactt attacatcat gattgatggc tatgccggaa agcagtgtga
     5881 ttataccttt gttgcaactg aaggtgtagc aatgccgtct gttgaaatta ctcctggtcc
     5941 ttctgttaac ctatgtcaag gtgagtcagt agaagtaaca gcttctggag gtagtggaac
     6001 ttatacgtgg aatgcagacc cagctttgag tgcaacaacg ggagcaaccg taaccatcac
     6061 accgccaaca acggtaggag catacaccta cgtcgttcaa tcttcgggtg gaacaacttt
     6121 ttgtccagaa aatagttcgg atacacttac agtgaatgta actgattgtg gtacttgtaa
     6181 cgttggttta tcattaactt ctaccgattg caatgctgca aacaatacgt atgacgcaac
     6241 tgtttctatt gcatttgcca atcaaccaac aacaggtgat ttgattttaa ctgcatgcga
     6301 tggctccact caaaccattc cagttgcatc gttgggaaca agttcctaca cggctaccat
     6361 tcctaattta agtttggcaa ctgccaactg tcaggttgtt gcatcgtttt ctgctacgcc
     6421 tgattgtgct gataccttag ttattacacc aagtgcagca caaacaccaa cgtttaccaa
     6481 tccaggaccg ttgtgtgtgg gacaagccta cacgttgcca actacatccg ataatggaat
     6541 tacaggtaca tgggcacctg catacaatgc taatcaaaca acaacttata cgtttacacc
     6601 aactggtaac gattgtgcaa caacggcaac tatgacgatt accattacca atgaaatcac
     6661 accaacgttt accaatccag ggccaatgtg tgtgggacaa acttatacat tgccaactac
     6721 atccgacaat ggaattacag gtacgtggac acctgcatac aatgccaatc aaacaacaac
     6781 atatacgttt acaccaacat caagcacatg tgcaacaaca gcaactatga cgattgatgt
     6841 agttaatgaa atcgtaccaa cgtttgatcc aatcactcca atttgccttg gcacggacta
     6901 tgcgttacca acaacatcaa ccaatggggt agtaggaact tggtcgccgg caatcaacaa
     6961 cacacaaaca acaacctata catttacacc aacctctagt tcgtgtgctg tttctactac
     7021 gttggttgtt gaaaccgatg aaacgctttg tcctgagcaa ccatttattg aattgcccaa
     7081 tgtttttact ccaaatggag ataatgtaaa cgaaacatac attctaacat taaaaggaat
     7141 tgaaagcatt gagtattgga tagtaaatcg ttggggcgag atggtttatc attctactag
     7201 tttaacagat ttctggaatg gaaaaaccaa aggtggttca tctgcagaag gtgtttattt
     7261 tattaaatac aaagctaaa
//
LOCUS       DAOQVQ010000071         7250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11341_length_7250_cov_3.523975, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000071 DAOQVQ010000000
VERSION     DAOQVQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11341_length_7250_cov_3.523975"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKN22_03485"
     CDS             <1..870
                     /locus_tag="PKN22_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41798.1"
                     /translation="FGTTVLEWEVVSANCGTTSDTIYIINSAAPSNANVQDTVYACAV
                     NQLNIATSVPIAGTGMWSTSSGATFADANSPVTTVSNLPEGWSQIIWTISSPGCASKA
                     DTMAVFKTGGQQFLTNDTTLCIEGFEKFVFTKTPTDSLQTVNWVFATGNALMNEITAD
                     SIELTGMVAGTTTLIYEVEHNLCPKETDTLKIVLTPCEDFEPVFPTVITPNGDGKNDL
                     FVIQNLENIYPNCQVTIFNRYGKVVFESTGYQTPWNGTFKGEKLPMGAYFFKLELNDG
                     SNKVYSGSISLIH"
     gene            884..1900
                     /locus_tag="PKN22_03490"
     CDS             884..1900
                     /locus_tag="PKN22_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS41799.1"
                     /translation="MKNKLIIFALACSTSVFAQQEMQFASSFQNPYLYNPAAGGLSSV
                     AQIDLVTRLQWMGIDGPTTINLSGHSVVGAPNSGVLDELKGGNKFLQPRPKVTVGGLK
                     HVVGGKIISEQIGVFQRMGVYGSYAIHLPVTKEFNIGAGIGLGWGNYRINPKRVTLYD
                     QVDDTYAMALGATSQQNILDATAGIVFYGKGLFFGFSATNLLKNKAKFSSVDTKSFYA
                     RHYNLTLSYGIKMGSSVLEPAVIGRFAAHAPANFDFGARFLYKNAIWLGVYGRTSNNV
                     IFQFGTTLIQNIYLSYSYELSVGKIRNAAGGTHEIQLGIYIGKRKAPKTDTKVDVQKE
                     QKSE"
     gene            complement(2125..2886)
                     /locus_tag="PKN22_03495"
     CDS             complement(2125..2886)
                     /locus_tag="PKN22_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNS41800.1"
                     /translation="MNNNSAYCFVSIAPVRNNPQDSSEMVTQLLFGEVVDILEITEKW
                     LKIKTFYDNYEGWIDPKHVKTLSKKEVTRWLDGIDFLHDKMTTIDTPWGEQQIVRGSF
                     VPFSSEQEFNIGNDVFKFTTPRAKVSNPSLIAIAKDYLNSPYLWGGKSPFGIDCSGFT
                     QVCFRLMGINLPRDASQQVEHGSLVNFEERMAGDVLFFHNDAGKIIHVGICLSENEII
                     HASGRVRVDFYDQKGIIHFTNECYTHHLHSIKRFL"
     gene            complement(2886..3461)
                     /locus_tag="PKN22_03500"
     CDS             complement(2886..3461)
                     /locus_tag="PKN22_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNS41801.1"
                     /translation="MTTGLLFNELEKITNENIQTIEEKFTQLSNEQLDWKPRQDYWNI
                     REVFAHLYEFAKYYHSTFSKKIDTTRFVEPTENFVSSPLGKSMWSAMKLGNAKNVKRK
                     MKSHKLYNPTIVPSIVTETVVHDFLTSQRELLKIINRAKGINIRKAKIKLANNNVIKF
                     RIGDAFLYVIYHNQRHIQQAINLLQHSKFPR"
     gene            3569..4552
                     /locus_tag="PKN22_03505"
     CDS             3569..4552
                     /locus_tag="PKN22_03505"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNS41802.1"
                     /translation="MLCIYHESTDPYFNVATDEFILKHLKEDCFMLWRNDNAIIVGKY
                     QNTLAEINYEYVKEHQMAVVRRISGGGAVYHDMGNLNFSFTQSGEDCNLTDFEKFTKP
                     IVDVIQQLGGDAYFSGKNDLMIDGMKIAGNAAHIYKNKILSHGCLLFASNIKDVGGAL
                     RINPMKYIDKAAKSVPKRVTNISEHLKVKITVEDFAQKIMEHVLSIYPDSKLYEFTED
                     DLRIIQQLRDEKYATREWNFGRSPDYTFQKAIRTKGGLLEMNMNVKKGGTIEAVKIYG
                     DFFSENGVEELEQALTGIQHDETAIRKALSQLDLSRYLSNIEVDELVGAMF"
     gene            complement(4649..7135)
                     /locus_tag="PKN22_03510"
     CDS             complement(4649..7135)
                     /locus_tag="PKN22_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41803.1"
                     /translation="MKPIILTSIVATMLHFTTFSFEKTLSWKFVNPKNQQEVILGTAG
                     SVQEALINANFLPDPFLGSNEKQFDWIEDYLWTFTSNFELSANDLSAQYIDIELPSVD
                     TYAQIYLNGTLILTCQNAFLPYRLTVNKYLKEGNNELKAVFTPPVLYHQKKYLKAKYK
                     LPAPNDVNKKIAIAPYTRKPQYQFGWDWALRMNTIGFNKPVKIVSYQENKILLSSVQT
                     LKVEHNKATLELAVQLAQEPTEHLVWNSKLFGTIHWKREGNWLKAHVTVEQPKLWWPV
                     NFGEQYLYNDEWQILNKHHQPIAAIEKNFGIRTSRLLQNADQFGTSYEIEINGKVIFC
                     KGGDVIPQDIFPARVTPEKIKQLVEQMSVANFNMVRIWGGGYYPDDVFYEECDKKGLM
                     VWQDFMFACSMYPGDAAFLENVKNEADYHIPRITSHPSVVLLNGNNEVDVAWKNWGFQ
                     LQYGLIGKSVIEIEKAYVDLFKKLLPERVAHFSTVPYIHTSPLSNWGKAEYYNHGSQH
                     YWGVWHGKDPIEDFGRKIGRFNSEYGFQSFPEYATLSKVIDKKDWNLDSDVMKLRQKS
                     YVGNGMIKKQSDILYGKTNDFDTFIYYSQLTQAKAVGIAIAGHRADWPRCSGTLYWQV
                     NDCWPAPTWSSVDYYNNWKALHYQAQKDFANIAVVATEKNSNQKSYYLVADACDTFST
                     TISFDFYDLNGNWLEQDSIILTIKPQSVNALPVFDMMQHQPNNYITKVSWNDDKNNRH
                     QRVFYNLVDKEQIAQNPEISMQLEQKANNVYTLEINTNKPLLECWIYAKDKSFHLKHN
                     FETLLAGKHVFEFTSTEKLVAEDIQLMFLK"
     gene            complement(7145..>7250)
                     /locus_tag="PKN22_03515"
     CDS             complement(7145..>7250)
                     /locus_tag="PKN22_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrophosphatase"
                     /protein_id="HNS41804.1"
                     /translation="ANQTGIDLEEALTKNLEKKTNRDYNRHKNNSKLS"
BASE COUNT         2243 a         1291 c         1378 g         2338 t
ORIGIN      
        1 tttggaacaa cggtattgga atgggaagtt gtttcagcca attgtggcac aacttcggat
       61 acgatttaca tcatcaattc agctgcacct tctaatgcca atgtgcaaga tacggtgtat
      121 gcatgtgcag ttaaccaatt gaatattgca acatcggttc caattgctgg aacaggtatg
      181 tggtcaacat ctagtggggc aacatttgcc gatgcgaact cgccagtaac aacagtgagc
      241 aacttaccag aaggttggag tcaaattatt tggaccatct catcacctgg ttgtgcctct
      301 aaagcggata caatggcggt atttaaaacg ggtggtcaac aattcttaac caatgacaca
      361 acgttgtgta ttgaaggttt tgaaaaattt gtattcacca aaacaccaac ggattccttg
      421 caaacggtaa attgggtatt tgcaacagga aatgcactaa tgaatgaaat tacagccgat
      481 tctattgaat taactggaat ggtggctgga acaactacct taatttacga agttgaacac
      541 aatttatgtc caaaagaaac ggatacccta aaaattgtgt taacaccttg cgaagatttt
      601 gaacccgttt tcccaacagt aattacacca aatggggatg gtaaaaacga tttgtttgta
      661 attcaaaact tggaaaacat ttacccgaat tgtcaagtga ctatttttaa ccgctatggc
      721 aaagtggttt ttgaatctac aggttatcaa acaccttgga atggaacgtt taaaggtgag
      781 aaattaccaa tgggtgctta tttctttaaa ttagaattaa acgacggttc aaacaaagtt
      841 tattctggat ctattagttt aatccattaa tccaactatg aaaatgaaaa ataaattaat
      901 catatttgca ctagcgtgtt caacaagtgt ttttgcacaa caagaaatgc aatttgcgtc
      961 ttcttttcaa aatccttatt tgtacaaccc agcagctgga ggattgtcta gtgtagctca
     1021 aatagatttg gttacacgcc ttcaatggat gggaattgac ggtccaacaa ccattaattt
     1081 aagcggtcac tctgttgtag gagcaccaaa tagtggtgtt ttagacgagt tgaaaggtgg
     1141 aaataaattt ttacagccac gcccaaaagt aactgttgga ggattgaaac acgtggtagg
     1201 aggaaaaatc atcagtgaac aaattggtgt ttttcaacgc atgggagtct atggaagtta
     1261 tgccattcat ttgccagtta caaaagagtt taacattgga gccggtattg gtttgggttg
     1321 gggaaattac cgtatcaatc caaaacgtgt aacattgtac gaccaagtag atgatactta
     1381 tgcaatggcg ttgggtgcta cttcgcaaca aaatattttg gatgccactg caggaattgt
     1441 tttttatggt aaaggtttgt tctttggatt tagtgctacc aatttattga aaaacaaagc
     1501 aaaattcagc tctgtggata ccaaaagctt ttatgcacgc cattacaact taacattgtc
     1561 gtatggaatt aagatgggta gcagtgtttt ggaaccagca gtaatcggac gttttgcagc
     1621 acatgcacca gctaattttg attttggtgc ccgtttctta tacaaaaatg caatttggtt
     1681 gggtgtttac ggtagaacaa gtaacaacgt tattttccaa tttggaacaa ctttgattca
     1741 aaatatctac ttgagttatt cgtatgaatt gagtgttggt aaaatcagaa acgcagctgg
     1801 aggcacacac gaaattcaat taggaatcta tatcggtaaa cgcaaagcac caaaaactga
     1861 tactaaggtt gatgttcaaa aagaacaaaa atcagaataa attaactgaa ttatttttat
     1921 aaagtaaagg gattttagtt gaaactttaa tccctttttt tacgaagata caattacaaa
     1981 agtcacctcc cttaatccct cctcaaggag ggaaagcggt ttacacttaa aagttaataa
     2041 gaactccaat ttttgaattt tatatccctc tgcgcccatt gcgccttctt tgcgcccttt
     2101 gcggttaaaa attctatgaa ttgcttacaa aaagcgtttg atagaatgca aatgatgcgt
     2161 ataacattca ttggtgaagt gaatgatgcc tttttgatca taaaaatcta cacgtacacg
     2221 tccactggca tgaattattt cattttcgga taagcaaata ccaacatgga tgatttttcc
     2281 tgcatcgttg tgaaaaaaca gcacatcacc tgccatgcgt tcctcaaagt taactaaact
     2341 tccatgctcc acttgttgag aagcatcgcg tggtaaatta atgcccatta aacgaaaaca
     2401 cacttgggta aaacccgaac aatcaatccc aaaaggcgat tttccacccc ataaatatgg
     2461 cgaattcaaa taatcctttg caatagcaat taacgatgga tttgaaactt tagcacgagg
     2521 agtggtgaac ttaaaaacat catttccaat attgaactct tgctcactag aaaaaggtac
     2581 aaaagaacca cgcacaattt gctgctctcc ccaaggtgta tcaatagttg tcattttatc
     2641 gtgcaaaaaa tcaataccat ccaaccaacg tgtaacttct ttcttcgaca atgtttttac
     2701 atgcttaggg tctatccaac cttcgtaatt atcataaaat gtcttgattt tcaaccattt
     2761 ttcggtaatt tcaagaatgt caacaacttc accaaataga agttgcgtta ccatttcact
     2821 gctgtcttgt ggattatttc gcacaggagc aattgataca aaacaataag ccgaattatt
     2881 attcattagc gcggaaattt ggagtgttgg agtagattga tagcttgttg tatgtgacgt
     2941 tgattgtgat aaatgacgta taaaaaggcg tcaccaattc taaatttgat gacgttgtta
     3001 ttggccaatt taatttttgc cttgcgaatg tttatgcctt ttgcacggtt gatgatttta
     3061 agcaattccc gttgactggt taaaaaatca tgaacaacag tttctgtaac aatcgaggga
     3121 acgatggttg gattgtacaa tttgtgcgac ttcatttttc gttttacatt ctttgcattg
     3181 cccaatttca tggcactcca catgcttttt cctaaaggcg aagaaacaaa attttcagtt
     3241 ggttcaacaa aacgtgtggt gtctattttt ttggaaaatg tagaatgata atattttgca
     3301 aattcataaa gatgagcaaa aacctcacga atattccaat agtcttgacg aggtttccaa
     3361 tccaattgtt cgtttgataa ttgcgtaaat ttttcttcaa tggtttgaat gttttcattg
     3421 gtgattttct ccaattcatt gaataataat ccagttgtca tcttttttat tttttgcctc
     3481 ataaaattat aaaaatcatt tggattggaa tgaaaaaaac taaaaatgat gttattttat
     3541 tgctaaattt gaaggaaata gaaattttat gttgtgtatc tatcatgaat cgaccgaccc
     3601 gtatttcaat gttgccaccg atgaattcat tctcaaacac ttaaaagaag attgttttat
     3661 gttgtggcga aacgacaatg ccatcattgt tgggaaatac caaaatacac ttgccgaaat
     3721 caattatgaa tatgtgaaag aacatcaaat ggctgttgtt cgacgtattt ctggtggtgg
     3781 agcagtttat cacgatatgg gaaaccttaa tttttcgttt acacaatcgg gagaggattg
     3841 taacctcaca gattttgaga aattcaccaa gcctattgtt gatgtcattc agcaattggg
     3901 tggagatgcc tatttttctg gtaaaaacga tttgatgatt gatggaatga aaatagccgg
     3961 aaatgcagcg cacatctata aaaataaaat actctcacac ggatgcttgt tgtttgcttc
     4021 caatattaaa gacgttggtg gtgccttgcg aatcaaccca atgaaataca tcgataaagc
     4081 agccaaatca gttcccaaac gtgttaccaa catttcggag catttaaaag taaaaattac
     4141 cgttgaggac tttgctcaaa aaattatgga gcatgttttg tcgatttatc ctgatagtaa
     4201 attgtatgaa tttaccgaag atgatttgcg catcattcaa caactacgtg atgaaaaata
     4261 tgccacacgg gagtggaatt ttggtcgctc acccgattat accttccaaa aagctatacg
     4321 taccaaaggc ggtttgttgg aaatgaacat gaatgttaaa aaaggcggta caattgaagc
     4381 agttaaaata tatggcgatt ttttctcaga aaatggagta gaagagctgg aacaagccct
     4441 tacaggtatt caacacgatg aaacggctat cagaaaagca ctatcccagc tagatttatc
     4501 tcgttatcta agcaatattg aagtggatga gttagttggc gctatgtttt aacaccatag
     4561 aataaacgcg tgctaattct aggtgctctg tttaaatggc attttatact aaaacaccac
     4621 ttctccgtcg aaatagatgg tgaataaact atttcaaaaa catcaactgg atatcttctg
     4681 ccaccaattt ttctgtagat gtaaactcaa aaacgtgttt tccagcaagc aaagtttcaa
     4741 aattatgttt taaatgaaac gatttgtctt tggcataaat ccaacattcc aacaagggtt
     4801 tattggtgtt tatttccaac gtgtaaacat tgtttgcttt ttgctccaat tgcatggaaa
     4861 tttcggggtt ttgtgcaatt tgttctttgt caacaagatt gtaaaaaaca cgttggtggc
     4921 ggttgttttt gtcatcgttc caactcactt ttgtaatgta attatttggt tggtgctgca
     4981 tcatatcaaa aacaggcaag gcatttaccg attggggttt aatggttaaa ataatgctgt
     5041 cttgctccaa ccaattgccg tttaaatcgt aaaaatcaaa agaaatggtg gtgctaaacg
     5101 tgtcgcaagc atcggcaaca agataataac ttttttggtt gctgtttttc tcggttgcaa
     5161 ctacggcaat attagcaaag tctttttgcg cttgataatg caaggctttc caattgttgt
     5221 agtaatccac actcgaccaa gttggggcag gccaacaatc gtttacttgc caatacaaag
     5281 tacccgaaca acgtggccaa tctgcgcggt gaccagcaat tgcaatgccc acagctttgg
     5341 cttgtgttag ttgagaataa taaatgaagg tgtcaaaatc attagttttt ccatacaaaa
     5401 tgtcggattg tttttttatc attccgttgc ccacatagct tttttggcgc aatttcatca
     5461 cgtcagaatc caagttccaa tcttttttgt caattacttt ggaaagtgtg gcatattctg
     5521 gaaaactttg aaagccgtat tcggaattaa aacgtccaat ttttcttcca aaatcttcaa
     5581 ttggatcttt tccgtgccaa actccccagt aatgttgcga accgtggttg tagtactcgg
     5641 cttttcccca attactcagc ggacttgtgt gaatgtaagg aactgtagag aagtgagcaa
     5701 cacgctcggg taatagtttt ttgaataaat ccacataggc tttttcaatt tctataaccg
     5761 atttcccaat taaaccatat tgcagttgaa atccccagtt tttccaagct acatccacct
     5821 cattgttgcc attgagcaaa accaccgatg ggtgagaagt tatgcgtggg atgtgatagt
     5881 ccgcttcatt ttttacgttt tctaaaaatg ccgcatcgcc tgggtacatg gaacaagcaa
     5941 acataaaatc ttgccaaacc atgagtcctt ttttgtcaca ttcttcataa aacacatcat
     6001 cgggatagta gccaccgccc caaattcgca ccatgttgaa attggcaaca ctcatttgct
     6061 ctaccaattg tttgattttt tctggggtaa ctcttgcagg aaaaatatct tgcggaataa
     6121 cgtcgccacc tttgcaaaag ataacttttc cgttgatttc tatttcgtag gaagtaccaa
     6181 attgatcagc gttttgcaac aaacgactgg ttctaattcc aaaattcttt tcaattgccg
     6241 caattggttg gtggtgtttg tttaaaattt gccattcatc gttgtagaga tattgctcac
     6301 caaagttaac tggccaccac aattttggtt gttcaactgt tacgtgagct ttaagccaat
     6361 taccttctct tttccaatgg attgtgccaa ataatttact gttccacaca aggtgttctg
     6421 ttggttcttg tgccaattga acggccaatt ccaaggttgc tttgttgtgt tcaactttaa
     6481 gcgtttgaac agatgaaagt aaaattttat tctcttggta gctaacaatt tttaccggtt
     6541 tattgaaacc aatggtgttc atacgcaaag cccaatccca accaaattga tactgtggtt
     6601 tgcgggtata aggtgcaatt gcaatttttt tgttgacatc gttgggagct ggtaatttgt
     6661 attttgcttt taaatatttt ttttggtggt ataaaacagg cggtgtaaaa acagctttca
     6721 attcgttgtt gccttctttt aggtatttgt tgacagtaag acgatagggt aaaaatgcat
     6781 tttgacacgt taaaatcaaa gtaccattga ggtaaatttg tgcataggta tccaccgatg
     6841 gtagttcaat atcaatgtat tgcgccgata aatcgttggc tgataactca aaattgctgg
     6901 taaatgtcca caaataatct tcaatccaat caaattgttt ttcgttgctt cccagaaatg
     6961 gatcgggtag gaaattagca ttaattaaag cttcttgaac acttccagcc gttccaagaa
     7021 taacctcttg ctgattttta gggttaacaa atttccaaga aagcgttttt tcaaatgaaa
     7081 aagtagtgaa atggagcatg gttgcaacaa ttgatgttaa tataattggt ttcatttcaa
     7141 tttattaaga gagtttactg ttgtttttgt ggcgattata atcgcggttt gtttttttct
     7201 ctaaattttt ggttaaggct tcctctaaat ctatgccagt ttggttagca
//
LOCUS       DAOQVQ010000072         7225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11390_length_7225_cov_3.783264, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000072 DAOQVQ010000000
VERSION     DAOQVQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11390_length_7225_cov_3.783264"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKN22_03520"
     CDS             <1..414
                     /locus_tag="PKN22_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNS41805.1"
                     /translation="FHKLLGKIMWDNVGMARNEAGLKEAIVEIKRIREEFYKDVFVPG
                     EMNEMNPELEKATRVADFMELAELFAVDALHRTESCGGHFREESQTEDGEALRDDANF
                     AYVAAWEYTGNPGEAVLHKEQLEFKAIELKQRSYK"
     gene            458..1210
                     /locus_tag="PKN22_03525"
     CDS             458..1210
                     /locus_tag="PKN22_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNS41806.1"
                     /translation="MKLTLKVWRQKNSDDKGRIETYKVDNINEHMSFLEMMDVLNEQL
                     IAEGKEPVAFDHDCREGICGMCSMFINGEPHGPGKGITTCQLHMRSFNDGDTIYIEPF
                     RSRGFPVIKDLIVDRSAFDRIQQAGGYVNVNTSGNTLDANAIPIPKDDADKAFDAATC
                     IGCGACVATCKNSSAMLFISAKVSQFALLPQGKVEAKSRVLAMVEQMDKDGFGNCSNT
                     GACEVECPKGISLENIARLNREFLDAAVKSDN"
     gene            complement(1518..>3496)
                     /locus_tag="PKN22_03530"
     CDS             complement(1518..>3496)
                     /locus_tag="PKN22_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41807.1"
                     /translation="KKIMKKIKIILFIFFIIVKTGFSQINVYQDVFKGGVTGDAFAPW
                     NLPFGGDFEIYIEPNSTIRKAYLFVTVYFSPDENILYINNNPVVLNAQDTFNNEYLYI
                     DGTDIRRMKTLMIDVTHIINPTQNIYPVSALIQPSTLSLGFFTYFYLYVAYENTLMQD
                     VCANVFVNNVQPSSNMLFSFGNLNPIDVIQNVGLALNSGEMCDTIKDGSFVKIDNTLL
                     GLIGGQDDGGLSICDGVSGSFYYQNGALFGLGNDIANSTMSGTDAIANIESYLNSSTA
                     FDLTFDYQSTLGSKTNPIHQLFLTYTTTCAPFDVTVTNDTIICKGEQLQLMATSANPN
                     ATFEWSAPTGGTSGNPAPGLSCTNCPNPVFSGDSSMFYTVRIWNNDSCSVVRPVHILV
                     KEPPVLPLLTIAKAVCGTASGSVLIQKPQETGVLYLISENGDTLTKPLNKGQNALQGG
                     LFGGNYTVYFQDSLGCRSGDSSFVIPTINNTVAGFTVSPQAGGAPLEVVINNTSVQAQ
                     NYSWYVNGALQNNPFTNFVADTSGTYQIMLVAWQNDSACADTAYATVNVYDSLIVHIP
                     NVFTPNNDGVNDSFGIHTNLPIHAQLNILNRWGEVVYNFQGNLTPGFNPLWSTPATDG
                     VYFYEVVFTLDDTIVNCQLQNCRLTKEGFITVVR"
     assembly_gap    3497..3506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3507..3820)
                     /locus_tag="PKN22_03535"
     CDS             complement(<3507..3820)
                     /locus_tag="PKN22_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS41808.1"
                     /translation="MNTDLGFAIRKYRELKGFTQQQMADFLGMAVSNYNKIENNKLEL
                     TVVRMMEIVKILKININVLLDEDNVQSVQLTVNDNKGVVTGNYNTNYFTEKLNDIINL
                     KN"
     gene            3933..>7225
                     /locus_tag="PKN22_03540"
     CDS             3933..>7225
                     /locus_tag="PKN22_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41809.1"
                     /translation="MKKIPILIMFLMFMGTQLLAQNSYWILAPKKIYFPPFGNPQETS
                     LPTSSSPEGYHGQAAQFGQNIQMDASGNILFFIVDDRIYDRNGEIITAVSDDQTVELK
                     SYERVMYQSVFTHPISSNGAASEVIVLPDKQDCNLFHIISTGFYTSPFNVCVSYGRLR
                     ISYKADGTPTTNQAIEMQYNENVGTSGNTGAYNTMLSIDKIIGVNNFEVVDLSHHRSP
                     ALAVVDNGNSYTLFFRNVLKTYRLTIENGNITYNDYVTNIGGQAPDRYEMEAIKLSNG
                     NYRLACVIGSVSNSLDTINILVRDIAPNGIEVAGSQKILPYKVSLFNGYARYISGLEF
                     NADGSRLFVTHLLYNGNSTLDYWDISGSPIKTILSNDSEIGISQIEMAKDGKMYIPRQ
                     NYLARISNVNTTPTINLTAQSISSYSPTLGIFDQQVPLRILQDQIDGSNVYDNVNPST
                     YHATSYSVQTNEVWDGVNNPIAPGSSIINIEKELRIKEGVSATIKNIVFQFAPGARLV
                     IENAVTGSQGAKLTLDHTTLTNMPICGKEDMWLGVEVWGNSTAAQGSLLNSVQGILQL
                     KNNSRIENAYIGVLVSRRDETSTGEPINISHNNNYAGGVLNASNSTFFNCQNSVAFYD
                     YNQTSVNTSRISSCDFIWDGLLKNPSVKPSIHIVIYQNNKVYVGANKFYQNTPNIYTN
                     KLDRGMGIFAYNSPFSVASSCSVVMPFGQDCSEANTIRSEFKNLSWGVRVFNFNNNPF
                     EVIRNEFNNCLYGVTSVYAKNQKVSRNNFNIHEDNAMKTWGIYVTSGSGYEITENYLK
                     PYNGNNALTYGVIIDNSGETHNEVYKNTFEGLYIGSQAQGVNGKSYVPGNNNNALGLQ
                     YKCNTFKSPITNADIAVAVNSRIDYEQGRMDGNSQSDALKNAARNSFSHTNGAYDFFL
                     SSTSQQVNYVHLADYAQTPINYTITSPAAVYPYMQIWNGTPLYYNQEACPTKYPASWW
                     PPIIVFPFGKSAQIDSLVKIIDNNETANLLNNIATAPNETYTYNKLMDASPYLSDEVL
                     LAYINSAVKDVTLKSVLIENSKLSDTVYNALKASNRSNALKYDIGQYQTGVSSMKKLV
                     DIINIKEETL"
BASE COUNT         2428 a         1212 c         1308 g         2267 t
ORIGIN      
        1 ttccataaat tattaggaaa aattatgtgg gacaacgtag gtatggcgcg taatgaagct
       61 ggcttgaaag aagcaattgt tgaaattaaa cgcattcgtg aagagttcta caaagatgta
      121 tttgttccgg gtgaaatgaa cgaaatgaac ccagaattgg aaaaagcaac tcgtgttgcc
      181 gactttatgg agttagcaga gttgtttgca gtggatgcat tgcaccgtac agaatcttgt
      241 ggtggtcact tccgtgaaga atcacaaaca gaggatggtg aagcattaag agatgacgct
      301 aattttgctt atgttgcagc ttgggaatat actggaaatc ctggtgaagc agtgttgcac
      361 aaagagcaat tagagtttaa agcaattgaa ttaaaacaac gaagttataa atagatagtt
      421 gcggagttca tccacaatta tcataaatct aagcataatg aaattaacat taaaagtttg
      481 gagacaaaaa aatagtgacg ataaaggtag aatcgaaact tataaagtcg ataatataaa
      541 cgagcacatg tcatttcttg aaatgatgga cgtgttgaac gaacaattaa ttgccgaagg
      601 gaaagaacct gtagcatttg accacgactg tcgtgaagga atttgcggta tgtgttcaat
      661 gtttatcaac ggtgagccac acggaccagg aaaaggaatt acgacctgtc agttgcacat
      721 gcgttctttc aacgatggag atacaatcta cattgaacca ttccgttcaa gaggttttcc
      781 agtaatcaag gatttaattg ttgaccgttc tgcatttgat agaatccaac aagcgggtgg
      841 atacgttaac gtaaacactt ctggaaacac gttggatgcg aatgctattc caattccaaa
      901 agacgatgca gacaaagcgt ttgatgcagc aacatgtatt ggatgtggag catgtgtggc
      961 aacttgtaaa aactcttctg caatgttgtt tatctctgct aaagtttctc aatttgcatt
     1021 gttgcctcaa ggtaaagttg aagcaaaatc acgtgtgttg gcaatggtag agcaaatgga
     1081 taaagacgga tttggtaact gttctaatac aggtgcttgt gaggtagaat gtccaaaagg
     1141 aatttcttta gaaaacattg cacgtttgaa cagagagttt ttagacgcag cagtaaaatc
     1201 ggataattaa tatccaattg aaatgactag tcctaaataa ccaatacaga ttattaaggc
     1261 aaaaataatt aattatgagg gtgctcgact tttcggacac cctcattttt gttatatgga
     1321 cttgcaaaat agctatttaa cattgcatgc catcacctca aaacgccatt aaatcttacc
     1381 ttgccattct tataaaactt tttttgttaa aaaaacgcaa ttgtaatttt ttaaaggatt
     1441 cgagtttccc tcctttgagg agggattaag ggaggtgacc tagaatcata aaataatcaa
     1501 ctaaatccca attaaaccta ccttaccacc gttataaatc cttcttttgt taaacggcaa
     1561 ttttgcaatt ggcagttaac aatcgtatcg tccaatgtaa ataccacctc ataaaaatac
     1621 acaccatctg ttgcgggcgt gctccacagt gggttaaagc ctggcgttaa gttgccttga
     1681 aaattgtaca ctacctcgcc ccagcggttg aggatgttta gttgagcatg aattggcaaa
     1741 ttggtgtgga tgccaaaact atcgttaaca ccatcgttgt tgggtgtaaa cacattggga
     1801 atatgaacaa ttaaactatc gtaaacattt actgttgcat aagccgtgtc tgcacatgca
     1861 ctatcatttt gccaagccac aagcatgatt tggtacgtgc ctgaagtgtc tgccacaaaa
     1921 ttagtaaacg ggttgttttg caaagcaccg ttaacatacc aactgtagtt ttgagcttgc
     1981 accgaagtgt tgttaattac tacttccaac ggtgcaccgc ccgcttgcgg actcaccgta
     2041 aaacctgcca ctgtgttgtt gattgttggt ataacaaaag aactatctcc acttctgcaa
     2101 cccaagctgt cttgaaaata aacagtatag ttgccaccaa aaagcccacc ttgcaatgcg
     2161 ttttgacctt tgttgagtgg ttttgttaaa gtatctccgt tttctgaaat tagatacagc
     2221 acgcctgttt cttgtggttt ttgaataagc acgctccccg atgctgttcc acacaccgct
     2281 tttgcgattg taagtagagg caaaactggt ggttctttaa ccaaaatatg cacaggacgc
     2341 acaacagagc aagaatcgtt gttccaaata cgcacggtat aaaacatgct gctatctcct
     2401 gaaaaaacag ggttggggca attggtgcaa ctcaagcccg gtgcagggtt gccacttgtg
     2461 ccaccagtag gcgccgacca ctcaaaagtg gcattggggt tggcagaagt tgccataagt
     2521 tgcaattgtt ctcctttgca aatgatggta tcattggtta cagttacatc aaaaggtgcg
     2581 caggtggtgg tgtaggtgag gaaaagttga tgaattgggt ttgtttttga ccccaaagta
     2641 gattgataat caaaagttaa atcaaaagct gttgatgaat tcaaatatga ttctatgttt
     2701 gcaattgcat ccgtaccgct cattgttgaa ttcgctatat catttcctaa accgaatagt
     2761 gcgccatttt gataataaaa actacctgag acaccatcgc aaatagatag tcctccatca
     2821 tcttgaccgc ctattaaacc taataaagta ttgtctattt tcacaaaact accatcttta
     2881 atagtgtcgc acatttctcc agaatttaaa gctaaaccaa cgttttgaat tacatctatc
     2941 ggattgagat tgccaaatga aaacaacata tttgaacttg gttgcacatt attcacaaaa
     3001 acattagcac aaacgtcttg cattaaagta ttttcataag caacatatag gtaaaagtaa
     3061 gtaaaaaatc caagagataa agtagatggt tgaattaaag cagaaactgg ataaatattt
     3121 tgcgttggat tgatgatatg ggtaacatct atcattagtg ttttcattcg tctaatatcg
     3181 gtaccatcaa tatacaagta ctcattatta aaagtatctt gcgcattaag aactactggg
     3241 ttattgttga tatataaaat gttttcatcg ggagaaaaat aaacagttac aaacagataa
     3301 gcttttctga tagtactatt aggttctata taaatttcaa aatcacctcc aaaaggcaag
     3361 ttccaaggag caaaggcatc acctgtaaca ccacccttaa aaacatcttg atagacattt
     3421 atctgtgaaa atcctgtttt cactattatg aaaaaaataa acaatataat ttttattttt
     3481 ttcataattt tcttaannnn nnnnnnttgt tttttaaatt aatgatgtca ttcaattttt
     3541 cagtaaaata attggtgtta taatttcctg taactacacc tttgttatca tttactgtta
     3601 actgtactga ttgcacatta tcttcgtcca ataaaacatt aatgtttatt ttcaatatct
     3661 ttactatttc catcatacgc acaacagtga gttctaactt gttgttttca attttattgt
     3721 agttggaaac agccattcct aaaaagtcag ccatttgttg ttgagtaaag ccttttaact
     3781 ctctatattt tcttattgca aatcctaaat ccgtattcat aagtgctaaa ttaaataaaa
     3841 aataaaaaca aaaataagaa ttttcataaa atgaaaaaaa ataaatgttt ttttttcgaa
     3901 tattgtcaaa taaactatta atacaattaa ttatgaaaaa aatacctatt ttgattatgt
     3961 tcttgatgtt tatgggaaca caactattag cccaaaattc ttactggatt ttagcaccaa
     4021 aaaaaattta ctttccccct tttggtaatc cacaagaaac ctccctcccc acctcctctt
     4081 cgccagaagg ttatcatggt caagcggcac aatttgggca aaacattcag atggatgctt
     4141 cgggcaacat tttattcttc attgtggatg atagaattta tgatcgtaat ggagaaataa
     4201 ttacggctgt gtctgacgat caaacggtgg aattaaaaag ttatgaacga gtaatgtatc
     4261 aatctgtttt tactcatccc atttcttcta atggagctgc ttcagaggtt attgtgttgc
     4321 ctgataaaca agattgtaat ttgtttcata taattagcac aggcttctat acatcgcctt
     4381 ttaacgtttg tgtttcgtat ggaagattaa gaatttctta taaagcagat ggcacaccta
     4441 caaccaacca agctattgaa atgcaatata acgaaaatgt aggtacttct ggtaatacag
     4501 gtgcttataa caccatgtta tctattgata aaataattgg agtaaacaat tttgaagtag
     4561 tagaccttag tcatcatcgt tcaccagcat tggctgttgt tgataatgga aactcttata
     4621 ctttattttt tagaaatgtt ttaaaaactt atcgattaac aatcgaaaat ggaaatatca
     4681 cctataatga ttatgtaaca aatattggtg gtcaagcacc agatagatat gaaatggaag
     4741 caataaaact ttctaacggt aattacagat tagcgtgtgt aatagggagt gtatctaatt
     4801 ctttggatac tataaatatt ttagttcgtg atattgctcc aaatggaatt gaagtagctg
     4861 gttcgcaaaa aatattaccc tacaaagtta gtttatttaa tggatatgca aggtatattt
     4921 ctgggttaga atttaatgca gatggttcac gattgtttgt aacacatctt ttatataatg
     4981 gaaatagcac tttagattat tgggatattt ctggcagtcc aataaaaacc atcttgtcaa
     5041 atgattctga aattgggata agtcaaattg aaatggcaaa agacggtaaa atgtatatac
     5101 cacgtcaaaa ctatttagca agaatttcca atgtaaatac cactcctaca ataaatttaa
     5161 cagctcaaag tatttctagt tattcaccaa cacttggaat ttttgaccaa caagtccctt
     5221 tacgcattct acaagaccaa atagatggga gcaatgtgta tgataatgta aatccaagca
     5281 cttatcatgc aacatcttat tctgttcaaa caaatgaagt ttgggatggt gtaaacaatc
     5341 caattgcgcc tggttcttca attatcaata ttgagaaaga gttgcgtata aaagaaggtg
     5401 taagtgctac aataaaaaat atagtatttc agtttgctcc gggtgctcgt ttggttatag
     5461 aaaatgctgt aactggtagt cagggcgcta aattaacatt agatcacaca acactaacca
     5521 atatgcctat ttgtggtaaa gaagatatgt ggttaggagt tgaggtttgg gggaactcaa
     5581 cagcagcaca aggtagtcta ttaaattcgg ttcaaggcat attgcaattg aaaaacaatt
     5641 caagaattga aaacgcatat attggtgtat tggtttcaag aagagatgaa acatcaactg
     5701 gtgagccaat caatatttct cataataata attatgcagg cggcgtgtta aatgcgagca
     5761 attcaacttt ttttaattgc caaaacagcg ttgcatttta tgattacaat caaacttctg
     5821 taaatacttc gcgtatttct tcatgtgatt ttatttggga tggtttatta aaaaacccta
     5881 gtgtaaaacc ttcaatacac attgttattt atcaaaacaa caaggtttat gttggtgcaa
     5941 ataagtttta tcaaaatact ccaaatattt acaccaataa attagatagg ggtatgggta
     6001 tttttgcgta taatagcccg ttttctgtgg ctagttcttg ttctgtagtg atgccttttg
     6061 gtcaagattg ttcagaagcg aacactatcc gttcagaatt taaaaacctt tcttggggtg
     6121 tccgtgtgtt taattttaat aacaatcctt ttgaagtaat aagaaatgaa tttaacaatt
     6181 gcctttacgg agttacttct gtttatgcta aaaatcaaaa agttagcaga aataacttta
     6241 acatccatga agacaatgcg atgaaaacat ggggcatcta cgttactagt ggttcaggat
     6301 atgaaatcac ggaaaattat cttaaaccat acaacggtaa taatgcatta acctatggag
     6361 ttatcattga taattctggt gaaacgcaca atgaagttta taaaaatact tttgaaggtt
     6421 tatacatcgg ttcgcaagca caaggtgtta atggtaaaag ttatgtgcca ggtaataaca
     6481 acaatgcttt aggtttacaa tataaatgca atacctttaa atcacctatc actaatgcgg
     6541 atattgcagt tgctgtaaat agtagaattg attatgagca aggtagaatg gatggcaatt
     6601 cacaaagtga tgcgctcaaa aatgctgcga gaaattcatt ttcacacacc aatggagctt
     6661 atgacttttt cttaagttca acaagccaac aagtaaatta tgtgcatcta gctgactatg
     6721 ctcaaacacc tatcaattat acaattacct ctccagcagc agtttatcca tacatgcaaa
     6781 tttggaatgg aacccctctt tattacaatc aagaggcgtg tcctaccaaa tatccagctt
     6841 catggtggcc tcctatcatt gtgtttccat ttggtaaatc tgctcaaatt gacagtctag
     6901 tgaaaataat tgataacaac gaaacagcta atttattaaa taacattgct actgcgccaa
     6961 atgaaactta tacttataat aagttaatgg atgcatctcc atatttaagt gatgaagttt
     7021 tattagctta tattaactca gctgttaagg atgttacact aaaatcggtt ttaattgaaa
     7081 atagcaaatt atcagacact gtttataatg ctttgaaagc atctaataga tccaatgcat
     7141 taaagtatga tataggtcaa tatcaaactg gggtttcttc catgaaaaag ctagttgata
     7201 taataaatat caaagaagaa acact
//
LOCUS       DAOQVQ010000073         7163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11515_length_7163_cov_4.038548, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000073 DAOQVQ010000000
VERSION     DAOQVQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11515_length_7163_cov_4.038548"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1186)
                     /locus_tag="PKN22_03545"
     CDS             complement(<1..>1186)
                     /locus_tag="PKN22_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNS41810.1"
                     /translation="GMSKLVHKDANNASLLTSITLSKDKVEALKTSLKENNNVYIMDI
                     SEMASSMLSTVKNDFNFLLYFSSSIVFLSLLVVYGRIELALFAFLPMLLSWVWIIGIT
                     NFFDIRFNFVNIILTTFIFGLGDDYSIFITDGLLQHYKTKKDSISSYKSAVILSGITT
                     IIGTGVLIFAKHPSIHSIAIISTLGIATIMFITLLVQPGVFHWFVTRRTAKKRGPITF
                     FTVIYSILLYTYFFLGSMFLSVLLIVFVLPFPAKKVKKQQFMNYLISKLAKSTLYAGV
                     HVKKVVLNPEKLDYSQPSIVIANHTSFLDILTVLMLHPKTIIMVKSWVYNSPVFGVFI
                     RYAGYIFVEKGTDTNIELVKKQLEKGYSLAIFPEGTRSVDGEIHRFHKGAFVLSKALN
                     VPI"
     assembly_gap    1187..1196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1197..2969)
                     /locus_tag="PKN22_03550"
     CDS             complement(<1197..2969)
                     /locus_tag="PKN22_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNS41811.1"
                     /translation="MIGRFCESITQFFIRHKLLFWSLFASITAILVIGVTQLKLDKDI
                     YSIFPKGKEFQEFSQVIQENNLNKQLVFSFKTPADEDQTIDQLESIASQLEHSFSKEI
                     GAVQIYRMVDQAQMISYLQNASISFLTDEDYQTIAQKTNNDSIAKALENVKERLSGAN
                     AVFLGDYFAKDPLSVLGKKMSQFNIQSDSSAYTVENGIVYSYDKKRTFIFADLLVDSK
                     NNAQLEVFDTRIHQFIAQQKIAKDLDLFGVYQISLANAQQIQKDTTLTSVISIALILL
                     LLIGYYRSFIIPLFFLLPAGFGMLAGLGLTGYIQPNINAISLATASVLLGIVLDYSFH
                     FYTHYKHSGNLLETVREIGTPLTIGSFTTIAAFAALLFTESVVLKNFGLISLCTLLGA
                     VLFTLTMLPVIIHTFNIKLAYKERATAPKKLSKLAFRLLLFGVVALTAFSLLKAQGLN
                     FDGDLNNLAYHPEQLKSKENAFTGINPQYEKRLYLFSRGTSLEQAKTASLDLYQHLET
                     NKNKFEIAEIISPAPFLIPQNTWDQQQNKWVEFWKQHPTVQQEIVSTGDQLGFSSTAF
                     TPFFESIAHPTPMDEQGAQLVEQMG"
     gene            3166..5169
                     /locus_tag="PKN22_03555"
     CDS             3166..5169
                     /locus_tag="PKN22_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HNS41812.1"
                     /translation="MEAALDKRYIEFDATGFSDEELLNIYKKLVLPRIIEEKMLILLR
                     QGKITKWFSGWGQEAISVGCAYAMESDEYILPMHRNLGVFTTREIPLNRLFSQFQGKE
                     NGFTKGRDRSFHFGTQAHKVVGMISHLGPQLGLADGIALASKLRKDKKTTLVFTGDGG
                     ASEGDFHEAVNVASVWDLPVIFAIENNQWGLSTPSREQFNCKQFTDKGIGYGMEGIQV
                     NGNNILEVIRTVRAIREKIKTDSKPVLLELMTFRMRGHEEASGTKYYPEGLQDKWKLQ
                     DPVDNYALFLKEKGILTAELEKEIHDSYKEEIQKNFDIAQAEPLITANLATELNDVYA
                     PFTQEVVAPKGATKEMRFIDAIQDGLKQSMEKYPELVIMGQDIAEYGGAFKVTEGFVD
                     LFGKERVRNTPICESAIVGAGLGLSIAGMKAVVEMQFADFVSCGFNQIVNNLAKLHWR
                     WGEKADVVVRMPTGANTAAGPFHSQSNEAWFFHTPGLKVVYPSTPEDAKGLLAAAIED
                     PNPVLVFEHKYLYRSQKAEVATDYYTTEIGKARTVRSGNDLTIITYGLGVIWAEEYLN
                     QHPEINAEVLDLRTLLPLDLDAIYAAAKKTGRVLVLHEDCMTGGIGGEIAALIAENCF
                     EHLDAPVKRVASIDTPVPFAVPLEQQFLPKVRIDEAIQQLMKY"
     gene            5229..6680
                     /gene="asnS"
                     /locus_tag="PKN22_03560"
     CDS             5229..6680
                     /gene="asnS"
                     /locus_tag="PKN22_03560"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNS41813.1"
                     /translation="MKRTKIVDVLTNTAVGSEVVVKGWVRAFRSNRFIQINDGSTINS
                     LQAVVEFENFEEALLKKITTGAAIGLTGKLIESQGAGQAVELQVSAIEIIGEANPDDV
                     QKTVMQPKKHSLEFLREQAHLRFRTNVFGAVFRIRHGVAFAIHQFFNERGFYYLHTPI
                     ITASDAEGAGEMFHVTNLDLEKLPKNEDGSINFKEDFFERPVNLTVSGQLEAELGALA
                     LGQVYTFGPTFRAENSNTSRHLAEFWMIEPEVAFNDLNDNMDLAEDMLKYICRYIVEN
                     YADDLAFLNEREQKEEQAKPANERNELSLIERLNFVINNDFKRITYTEAIDILMNSNH
                     YKKKKFKYDVNWGTDLQSEHERYLVEKEFKRPVIITNYPASFKAFYMRRDDNCEPGKE
                     TVAAMDILFPGIGEMVGGSQREERLDVLKEKCAAFGIHEEDLWWYLDTRKFGSVPHAG
                     YGLGFERMVMFVTGMSNIRDVIPFPRTPGNAEF"
     gene            complement(6873..>7163)
                     /locus_tag="PKN22_03565"
     CDS             complement(6873..>7163)
                     /locus_tag="PKN22_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41814.1"
                     /translation="YTITSSGKWDFLKGVSKDFKNNERVVFNTLKKVVTVSSLYTTSS
                     SDTYKDGEVSTVYVITESKGKSLKLSAEGSNVKSTTNHTLTINTKETYSLQQ"
BASE COUNT         2337 a         1185 c         1467 g         2164 t
ORIGIN      
        1 aatgggaaca ttcaatgctt ttgacaaaac aaatgctcct ttgtgaaaac gatgtatttc
       61 tccatccaca ctacgtgtac cctcaggaaa aattgccaaa gagtagcctt tttctagttg
      121 ctttttaacc aattcaatgt tggtgtctgt gcctttttct acaaaaatat aaccggcata
      181 gcgaatgaat actccaaaaa caggtgaatt atacacccaa ctttttacca taataatcgt
      241 ttttggatgc aacatcaaca cggttaatat atctaaaaac gaagtatgat ttgcaataac
      301 aatggatggt tgcgaataat ccaatttttc ggggttgagt accacttttt ttacgtgaac
      361 acctgcatac aacgttgatt ttgccaactt agagatgagg tagttcatga attgttgttt
      421 ttttactttt ttagccggaa atgggagcac aaaaacaatc aacaaaaccg ataaaaacat
      481 acttcctaaa aagaaatagg tgtacaataa aatgctgtaa attaccgtaa aaaatgtaat
      541 gggaccgcgt ttttttgccg ttcttcgggt aacaaaccaa tgaaaaacac ccggctgaac
      601 caataaggtg atgaacataa tggtggcaat tcccaacgtg ctgatgatgg caatggaatg
      661 aatggaagga tgtttggcaa aaatcagcac gcctgtacca attatggtgg taattcccga
      721 taaaatcact gccgatttgt aggatgaaat gctgtctttt ttggttttgt agtgctgcaa
      781 caatccgtcg gtgatgaaaa tactataatc gtcgcccaac ccaaaaataa aagtcgttag
      841 aataatgttg acaaaattaa atcgaatgtc aaaaaagttg gtaataccaa taatccacac
      901 ccacgacaac aacatgggca agaaagcaaa caatgctaat tcaatgcggc cgtaaaccac
      961 caacaacgat aaaaacacaa tggatgatga aaaatacagc aagaaattaa aatcattttt
     1021 caccgttgat agcatacttg acgccatttc ggaaatatcc atgatgtaca cgttgttgtt
     1081 ttctttcaac gatgttttca aagcctcaac tttatctttt gaaagtgtaa tactggttaa
     1141 aagtgaagca ttgttagcgt ctttgtgcac caatttcgac atgcccnnnn nnnnnngccc
     1201 atttgttcca ccaactgagc accttgttca tccattgggg ttgggtgtgc aatggattca
     1261 aaaaatggtg taaatgcggt tgaagaaaac cctaattgat ctccagtgga tacaatttct
     1321 tgttgaaccg ttggatgttg cttccaaaat tccacccatt tattttgttg ttgatcccac
     1381 gtgttttgag gaattaaaaa tggtgctggc gaaataattt ccgcaatttc aaatttattt
     1441 ttgttggttt ctaaatgttg gtacaaatca agcgatgctg ttttggcttg ctcaagagag
     1501 gttcctctgg aaaataaata caaccttttt tcgtattgtg gattgatgcc tgtaaatgca
     1561 ttttctttgg attttagttg ctcgggatga taggccaaat tattcaaatc accatcaaaa
     1621 tttaatcctt gggctttcaa taaactaaat gctgtgaggg caacaactcc aaaaagcaat
     1681 aaacggaatg ccaattttga taatttttta ggcgctgttg cacgttcttt atacgccaat
     1741 ttaatgttaa aggtgtgaat aattaccggc agcatggtga gcgtaaacag cacagcaccc
     1801 aacaacgtac acaaagaaat caatccaaag tttttgagta ctaccgattc ggtaaacaac
     1861 aaagctgcaa atgctgcaat ggtggtaaaa ctaccaatgg tcaacggagt gccaatttca
     1921 cgcacggttt ccaataaatt accgctgtgt ttgtaatggg tgtaaaaatg aaaagaataa
     1981 tccaacacaa ttcccagtaa aacgcttgca gttgctaaag aaatggcgtt gatattgggt
     2041 tgaatgtagc ccgtaagtcc caatcctgcc aacattccaa atccagcagg gagcagaaaa
     2101 aacaaaggaa tgataaaact acggtaatag ccaattaata acaataaaat caacgcaatg
     2161 ctaataacac tggtaagcgt ggtgtctttt tgaatttgtt gcgcgttggc aagtgaaatt
     2221 tgatacaccc cgaacaaatc aaggtctttt gcaatttttt gttgagcaat aaattgatgg
     2281 atacgcgtat caaagacttc caattgcgca ttgttttttg aatcgaccaa taaatcggca
     2341 aaaataaagg tgcgtttttt atcgtagctg taaacaattc cgttttcaac cgtgtatgca
     2401 ctcgagtcgg attgaatgtt gaactgactc atttttttgc ccaaaaccga aagtggatct
     2461 tttgcaaaat aatcgcctaa aaaaacagca ttggcacccg aaagtcgctc ttttacgttt
     2521 tctaacgcct ttgcaattga atcgttgttt gttttttgag ctatggtttg gtaatcctcg
     2581 tcagttaaaa aagaaattga cgcattttgg agatagctaa tcatttgtgc ttggtccacc
     2641 attcggtaaa tttgcacggc tccaatttct ttagaaaaag agtgttcaag ttgactggca
     2701 atggactcta actggtcaat cgtttgatct tcatccgctg gcgttttaaa agaaaaaacc
     2761 aattgtttgt tgaggttatt ttcttgaata acttgactaa attcttgaaa ttctttccct
     2821 tttggaaaaa tggaatagat gtctttatcc aatttaagtt gggttacgcc aattactaaa
     2881 atagctgtaa ttgaagcaaa taagctccaa aatagaagtt tatggcgaat aaaaaactgc
     2941 gttatggatt cacaaaagcg gccgatcatt ttctaaatat tgagccgaag atagtgaaaa
     3001 tgttaaaatt gtaagatttt agggtgtatt tttcaagaag atacggcaaa gagcgaatgg
     3061 ttgttgaata ttttttaaaa ttgctgattt cagctatttt ttttaaccat ttagcggaat
     3121 tttaattgaa taaaacttag ctttgtattt tattaattta ttccaatgga agcagcatta
     3181 gataaacgat atattgagtt cgacgctaca ggatttagcg atgaagaatt attaaacatc
     3241 tacaaaaaac ttgttttacc gcgcattatt gaagaaaaaa tgttgatttt gctgcgtcaa
     3301 ggtaaaatta ccaaatggtt ttcgggatgg ggacaagaag ctatttctgt tggatgtgct
     3361 tatgccatgg aaagcgatga gtacattttg ccgatgcacc gtaatttggg tgtttttaca
     3421 accagagaaa ttccattgaa ccgtttgttt tctcaatttc aggggaaaga aaatggattt
     3481 acaaaaggtc gcgatcgttc gtttcacttt ggtacacaag cacataaagt ggtggggatg
     3541 atttcgcatt tgggtccaca attaggcttg gcagatggaa ttgcattggc ttcaaaatta
     3601 cgtaaagaca aaaaaacaac cttagtattt actggtgatg gtggtgcatc tgaaggcgat
     3661 tttcacgaag cagtgaacgt ggcgtcggtg tgggacttgc ctgtgatttt tgcaatagaa
     3721 aacaatcaat ggggactttc aacaccgtcc agagaacagt ttaattgcaa acaatttacc
     3781 gacaagggaa ttggctatgg aatggaaggc attcaagtaa atgggaataa cattttggaa
     3841 gtaattcgta cggtgcgtgc cattcgtgaa aaaatcaaaa cagattctaa gcccgtgttg
     3901 ttagagttga tgacttttag aatgcgtggt cacgaagaag catcaggtac aaaatactat
     3961 ccagaagggt tgcaagacaa atggaaattg caagatccgg ttgacaatta tgcattgttt
     4021 ttgaaagaaa aaggcatttt gactgctgaa ttggaaaaag aaattcacga ttcatacaaa
     4081 gaagaaattc aaaaaaactt tgatattgca caagcagaac cgttgattac agccaacttg
     4141 gctaccgaat taaacgatgt gtatgcacca tttacgcaag aagtggtggc accaaaagga
     4201 gcaaccaaag aaatgcgttt tattgatgcc attcaagacg ggttgaaaca atctatggaa
     4261 aaatacccag aattggtcat tatgggacaa gacatagccg aatatggtgg tgcatttaaa
     4321 gtaaccgaag gttttgttga tttgtttgga aaagaaagag tgcgcaatac accaatttgt
     4381 gaatcggcaa ttgtaggtgc aggtttgggc ttatctattg ctggaatgaa agctgttgtt
     4441 gaaatgcaat ttgcagattt tgtttcgtgt gggtttaatc aaattgtcaa taatttagca
     4501 aaattacact ggcgttgggg cgaaaaagcc gacgttgtag taagaatgcc aacaggggca
     4561 aatacggcag ctggaccgtt tcactcacaa tccaacgaag catggttttt ccatacacca
     4621 ggactaaaag tagtatatcc atctacgcca gaagatgcca aaggtttgtt ggcggcggct
     4681 attgaagatc ctaatccggt gttggtgttt gaacacaaat atttatacag aagccaaaaa
     4741 gcagaagttg caaccgatta ttatacaacc gaaattggca aggcaagaac agtaagatct
     4801 ggaaacgatt taaccatcat cacgtatgga ttgggtgtaa tttgggctga agaatacttg
     4861 aatcaacacc cagaaatcaa tgccgaagtg ttggatttga gaaccttatt gccgttggat
     4921 ttagatgcaa tttatgcagc tgcgaaaaaa acaggacgcg ttttggtgtt gcacgaagat
     4981 tgtatgacag gcggtattgg tggcgaaatt gctgcgttga ttgccgaaaa ttgttttgaa
     5041 cacttagatg cacctgtaaa acgtgtagct tcaattgata cgccagtgcc gtttgctgtg
     5101 ccgttagaac aacaattctt accaaaagta cgtatcgatg aggcgattca acaattgatg
     5161 aaatattaaa aaaattcaac aaattgttaa attgaaatta tatttgtaga aaaacagctt
     5221 atttaaacat gaaaagaaca aaaattgttg acgtattgac caacactgct gtaggctcag
     5281 aagttgttgt taaaggatgg gtaagagctt ttagaagcaa ccgatttatt caaattaacg
     5341 acggttctac gattaattca ttgcaagcag tggtggaatt cgaaaacttt gaggaagccc
     5401 ttttgaaaaa aattacaaca ggtgcagcta ttgggttgac aggtaaattg attgaatcac
     5461 aaggtgcagg acaagcagtt gaattgcaag taagtgccat cgaaattatt ggagaagcca
     5521 atccagatga tgtgcaaaaa acagtaatgc aacctaaaaa acacagttta gagtttttgc
     5581 gtgagcaagc ccatttgcgt tttagaacca atgtttttgg agcagtgttt cgcattcgtc
     5641 atggagttgc ctttgctatt catcaatttt ttaatgagcg aggattttat tacttgcaca
     5701 ccccaattat tacagcttca gatgcagaag gggcagggga gatgtttcat gttaccaatt
     5761 tggatttaga aaaattacct aaaaacgaag atggttccat taattttaaa gaagatttct
     5821 ttgagcgtcc agtaaattta actgtatctg gacaattaga ggctgaattg ggagctttgg
     5881 cattgggaca agtttatacg tttggtccaa cgtttagagc agaaaattcc aacacttctc
     5941 gtcacttggc agaattctgg atgatagaac cagaagttgc tttcaacgat ttgaacgata
     6001 atatggattt ggctgaagac atgttgaaat acatttgccg ttacattgtt gaaaattatg
     6061 cagatgattt ggcattctta aacgagcgtg agcaaaaaga agaacaagca aaaccagcca
     6121 acgaacgaaa tgaattgtcg ctgattgaac gtttgaattt tgtcatcaac aacgatttta
     6181 aacgcattac ttatacagaa gccatcgata ttttgatgaa ttccaatcat tacaagaaaa
     6241 agaaattcaa atacgatgtg aattggggaa ctgatttgca aagtgagcac gaacgttact
     6301 tggttgaaaa agaattcaaa cgtccagtaa ttatcaccaa ctatccagca tcgttcaaag
     6361 cgttttatat gcgccgcgac gataactgcg aaccaggcaa agaaacggtt gcagccatgg
     6421 atattttatt cccaggaatt ggtgaaatgg tgggaggttc tcaacgtgaa gagcgtttag
     6481 atgtgttgaa agaaaaatgt gccgcttttg gtattcatga agaagatttg tggtggtatt
     6541 tggatacccg caaatttggt agtgtgccac atgctggtta tggtttagga tttgaacgta
     6601 tggttatgtt tgttacagga atgtcaaaca ttcgtgatgt gattcctttc ccacgcacac
     6661 caggaaacgc agaattttaa taaaaataat tgattttgta tataacgcct ttagctgttg
     6721 ctagaggcgt ttttttatgt aaaaggcatt cgttactctt tttttatgaa ttatgtttta
     6781 tcgttacaag tttattgatc atatatatcg ataaattaaa gcataaaaaa aggcgagttt
     6841 caagctcgcc tttcaaatac tgttgatact tcttattgtt gtaaactata tgtttcttta
     6901 gtgttaatag ttagtgtatg atttgtagtt gatttaacat ttgaaccttc agcagacaat
     6961 ttcaaagatt tacctttaga ttctgtaatt acataaactg tagaaacttc accgtctttg
     7021 tatgtatcag aagatgaggt tgtgtataaa gaacttactg tcacaacttt tttcaaagtg
     7081 ttgaaaacaa ctctttcatt gtttttgaaa tctttactta caccttttaa gaaatcccat
     7141 ttacctgaac ttgtgatagt ata
//
LOCUS       DAOQVQ010000074         7063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_11743_length_7063_cov_3.549515, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000074 DAOQVQ010000000
VERSION     DAOQVQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11743_length_7063_cov_3.549515"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..486
                     /locus_tag="PKN22_03570"
     CDS             178..486
                     /locus_tag="PKN22_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41815.1"
                     /translation="MAKLIGFPHVKQQNIRPLQTLQNVYVYHSSSKGDIPVNKMNEYH
                     LINAFRKHLSLALKSYLIETKNIREMVNKVNIVEIEDLITSNEVLNELYNEAQKRGLA
                     "
     gene            483..1073
                     /locus_tag="PKN22_03575"
     CDS             483..1073
                     /locus_tag="PKN22_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41816.1"
                     /translation="MTFFKLQNELELNLGYNPYKAFKNKDSEVIPYNLTFTYAEIHPT
                     LVYNVLLTIRKSNISKFVDLYDSNTNNLYSDVYLSAVIGHIIGRTAKTKGVFNVTSTA
                     YIEYENGKDLLDAFDDLSNKMEYNYFHCYKRLSNQHTILYSKMDDQYLLNAFFERMYQ
                     YMVKQPYSYNDFEVFSLRKIREISPEVDGILTYLEE"
     gene            1191..1817
                     /locus_tag="PKN22_03580"
     CDS             1191..1817
                     /locus_tag="PKN22_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41817.1"
                     /translation="MQEGMMEKFTIKIEPKMYAIVSINNKVVACPMIQFGKQTFICCG
                     DNKIEIIAYIPATKLKYFNVNNDFLQVYYELVNENTVNQLFGFLTNIYIAESYRRSLN
                     DTPEDSNSLFEDFINFFAYMAEMSHIEKGVPLIYLSDSMRGAIAQILHKHDCVDFAYF
                     ITPIISKGIEKFNNKLDGKNDEDIVKVGDEELNLNEIDLDFLGGLFSG"
     gene            1810..2046
                     /locus_tag="PKN22_03585"
     CDS             1810..2046
                     /locus_tag="PKN22_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41818.1"
                     /translation="MDELEFPDTEVSEIEIAIEDINLIFDREIENLLETEDEEKKEQA
                     LRLFQSKKLAIGAIKYSTIARTAMIHLRTILSVQ"
     gene            2320..3852
                     /locus_tag="PKN22_03590"
     CDS             2320..3852
                     /locus_tag="PKN22_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNS41819.1"
                     /translation="MLSQEQQQFTDLAVSGQNTIASATAGAGKTFTLAAAANSMIAAK
                     PNIKGVALTFSKPLADELQRKLPSAITGKTIHSLCYATVQKALKSFGGGKLVVDERKY
                     RKLLQDKDIWNDESDGYMEIIENLQKLPFTINELTEFINTNYPEETKRVVFDILSQGY
                     TQFTEEGTITYNDMVWYPVLKDYSKYAWRNQVVFLDETQDTSALYLKAIQMHSNSNSL
                     FLAVGDQQQTVHAWNGIPLDRFSQIQNELNATQVRFNRTYRLPQEHVDLLYQLNLTDD
                     IVTENKNSGNINNIKLEKIYSTAVPGDLILSRFHAGKKSNDVSLVEVGLGLLRQGKPI
                     RYVRFDPVKYIEMFVKFVNKAKINNLTIGLVKWEEKLTEIARNTCAKKGNDLNYEDLR
                     SKMELVDQKVETVQLHLNLYQGNDGNSFITFVKQLYDRNRSDNAIILSSAHAAKGLES
                     NNVYILNPHQFMSFRREPTVEEYVSEANVLFVALSRSKSNLNFIGEKPIAVKEQYLNY
                     YF"
     gene            3862..4221
                     /locus_tag="PKN22_03595"
     CDS             3862..4221
                     /locus_tag="PKN22_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41820.1"
                     /translation="MNTLTVKQLKSEQFKSVVYQISEAIKLIGDEAIYYLPSEEPYIS
                     LVMKLEEFKNIDFDKIDYIKVNWLTLAEYLIDVLLDGKIIDEELEMEINSLREENSYL
                     IGEIHELDQGQDFRLCD"
     gene            complement(4549..5181)
                     /locus_tag="PKN22_03600"
     CDS             complement(4549..5181)
                     /locus_tag="PKN22_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41821.1"
                     /translation="MSTKFQKCIYCYDELEIHHISLHQRQCYLNPTNLKKICDYFLSG
                     IENHKLLKRANFYRWAQKEGILTSISIANRLKAKKWSHALFQLLIYGYLHKFIDYEYV
                     EIILYIISSGSLWFSPEDFNKLYQESLDKEYKNKGITRDDLHYNYWLLLMCILDRSTR
                     DLELNTGDLDENKEEVDLNDAVSFLKDFAPDLLLTKLRNNKLSQDAIDLI"
     gene            5306..6295
                     /locus_tag="PKN22_03605"
     CDS             5306..6295
                     /locus_tag="PKN22_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41822.1"
                     /translation="MFYSELPTHKFNNTKLTTNFHNLFPEGFYLTAKDRPSASSAMCQ
                     RQVALQLTIDKTFVKQPKALNDYARIGNAIEDGVLSRYRKANQLFLSQWKIPEALTSL
                     NFNIGGIIDAFLQIENTLILLDIKSVGSVESEPTISLSSNEVAMLMQGEPLLFHPDDN
                     RMKNSVEKGIKEAHLSQLQLYAAVTGFDEVYIQLMSRRVQDKFEFDNTAPTTKFEQIP
                     VDISTLERRVAIVMYGIECKKRGYIPEPLNGIKKSHCADAFCSFQDFCWKDKTLSLKE
                     NLQHIPEKVGSDLKKECIDQAKEYISKRNERYELTLSLITDERNRRLKSKVEL"
     gene            6299..6781
                     /locus_tag="PKN22_03610"
     CDS             6299..6781
                     /locus_tag="PKN22_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41823.1"
                     /translation="MKVYLDIDGVISDFSAGLTKIYPEFINHDQTSYDLPSYVDLDTI
                     YNDKTFWMFLPVQDRPTVEINGLVSHRPFEKYITEFWLHINKFPKIPVYHVKSSHEKA
                     ELLINLKCDLYVDDKVSTFYECNSSGIRTLLYNQPWNRNVKTDMRINKLKELEKWLQS
                     "
     gene            6766..>7063
                     /locus_tag="PKN22_03615"
     CDS             6766..>7063
                     /locus_tag="PKN22_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41824.1"
                     /translation="MVAILAGEKVLYVYYYNTEQVFDKLPEADLYLPLEDNIAKHIIK
                     ETMWGKYLLEVLVTWNTDYPIYTPIALQVAHTSLGIVGEIAEILGTSETAIQTQL"
BASE COUNT         2635 a          920 c         1197 g         2311 t
ORIGIN      
        1 aatattgaac caggaattaa tacaggatgg ggaattttac aaagacatga acaaattatt
       61 tataatgaag ctcaacatag ttttgtttat ttattggatg ttcaatagca atttgacaaa
      121 gtaacaaacc aatgttatac taagttatag taacgttaat taagaaagga agaaaatatg
      181 gcaaagttaa ttggattccc acatgtaaaa caacaaaaca ttcgaccatt gcaaacatta
      241 caaaatgtgt acgtttatca ttcaagttca aagggtgata ttccagttaa taaaatgaat
      301 gaatatcatc ttattaatgc ttttagaaag catcttagtt tagctttaaa aagttattta
      361 attgaaacaa agaacattcg tgaaatggtg aataaggtta atattgttga aattgaagac
      421 cttattactt ctaatgaagt actaaatgaa ctttacaatg aagcgcagaa aagaggtttg
      481 gcatgacatt tttcaaattg caaaatgaat tagaattgaa tcttgggtat aacccttaca
      541 aagcgtttaa aaataaagat agtgaagtaa ttccctataa tttaacattt acatacgctg
      601 aaattcaccc aacgttagta tataatgtgc tgttgacaat tagaaaatca aatatcagca
      661 agtttgttga cttgtatgat agtaacacaa ataatcttta ttctgatgtt tatttatctg
      721 ctgtaattgg tcatattatt ggacgaacag caaagacaaa aggtgttttt aacgttacat
      781 caacggcata tattgaatat gaaaatggca aagatttgct tgatgctttt gatgatttat
      841 cgaataagat ggaatataat tattttcatt gttataaacg gttgagtaat caacacacta
      901 ttctatattc taaaatggat gaccagtatc ttttgaacgc attttttgaa cgaatgtatc
      961 aatacatggt aaagcaaccg tattcgtata atgattttga agtttttagt ctaagaaaaa
     1021 ttagagaaat ttcacctgaa gttgatggta ttttaacgta tcttgaagaa taaatcaata
     1081 attttgtatt tcctgctctt tctatttttg ttttatgcaa cattttgtga tgaaaaacaa
     1141 caagagaaat tgcaggaaat acaatacaca gaatgtttca taagtttagt atgcaagagg
     1201 gaatgatgga aaaatttacg attaaaattg agccaaagat gtatgcaatt gtttcaatta
     1261 ataataaagt tgttgcttgc ccgatgattc aatttgggaa acaaacattt atttgttgtg
     1321 gggataataa aattgaaatt attgcataca ttcctgcaac taaactaaaa tatttcaatg
     1381 taaacaatga ttttttacaa gtttattacg aacttgtgaa cgaaaataca gttaatcaat
     1441 tatttggatt tttaactaat atttatatag cagagtcgta cagacgttct ttaaatgata
     1501 cacccgaaga ttcaaatagt ttatttgaag attttattaa tttctttgcg tatatggctg
     1561 aaatgtcaca tattgaaaaa ggtgtgccat taatttactt atctgactca atgagaggtg
     1621 cgatagcgca gattttacat aaacatgatt gtgttgactt cgcttacttc atcacaccaa
     1681 tcatatcaaa aggtattgaa aagtttaata ataaattaga tggtaagaac gatgaagata
     1741 ttgtaaaagt tggtgatgaa gaattgaatc tcaacgagat tgatttagat tttttaggag
     1801 gactttttag tggatgaatt agagtttcct gatacagaag tatcagaaat tgaaattgca
     1861 atagaagata ttaatttgat ttttgataga gaaattgaaa acttgcttga aactgaagat
     1921 gaagaaaaga aagagcaagc tcttagatta tttcaatcta agaaattggc aataggagca
     1981 atcaaatact ctaccattgc aagaacagca atgatacatc ttagaactat tttaagtgtg
     2041 caataagcta taccaatcat aagggggaat cttatgaatg aaaaagatgc acaagtttta
     2101 attaatgcaa tttttgaaaa agttaagaag ttataacagc aatattctat ccctggaata
     2161 gataaagctt ttattgatgg tgaaataaat ggattgtact ggactatctc attaattgaa
     2221 aacgctactt acggttaact ttttgccatc gatttgacaa actccaaacc tgtggtataa
     2281 ttttcttata ataaataatt ataccacagg agataaaaaa tgctttcaca agaacaacaa
     2341 caatttactg accttgctgt ttctggtcaa aatactatcg ctagtgcaac cgctggtgct
     2401 ggaaaaacat tcactttggc tgctgctgca aatagtatga ttgctgcaaa accaaatatc
     2461 aaaggtgtag cgttaacatt ttctaagccg ttagcagatg aattacaacg aaaattacct
     2521 tctgcaatca caggtaaaac aattcatagt ctatgttatg ccactgttca aaaggcgtta
     2581 aagtcttttg gcggtggcaa gttagttgta gatgaaagaa aataccgcaa acttcttcaa
     2641 gataaagata tttggaatga tgaaagtgat gggtatatgg aaattattga aaatctacaa
     2701 aagcttccat ttactattaa cgaacttaca gaatttatta atactaatta ccctgaagaa
     2761 acaaaaagag ttgtgtttga tatattgagt caaggataca cacaatttac agaagaagga
     2821 acgattactt acaatgatat ggtatggtat ccggtgttaa aagattactc aaaatatgct
     2881 tggagaaatc aagtggtatt tttggatgaa acacaagata caagcgcatt atatcttaaa
     2941 gcaattcaaa tgcatagtaa ttcaaattca ttatttttag cggtaggtga ccaacaacaa
     3001 acagttcatg cttggaatgg tattccatta gatagatttt ctcagattca gaatgaactt
     3061 aatgcaacac aagtacgttt taatcgtact tatcgtctac cacaagagca tgttgatctt
     3121 ctttatcaac ttaatttgac tgacgatatt gttactgaga acaaaaattc aggaaatatt
     3181 aataatatta agttagaaaa aatttatagt actgctgtac ctggcgactt aattctatct
     3241 cgttttcatg caggaaagaa aagtaatgat gttagtttag ttgaagttgg tttaggttta
     3301 ctacgacaag gtaaaccaat tcgatatgtt agatttgatc cagttaagta cattgaaatg
     3361 ttcgtaaagt ttgtaaacaa agcaaaaatt aataatctaa caattggatt agtgaagtgg
     3421 gaagagaaat taactgaaat tgctcgtaat acttgcgcaa aaaaaggtaa tgatttaaat
     3481 tatgaagact taagaagtaa gatggagtta gttgaccaaa aggttgaaac agttcaatta
     3541 catcttaatc tttatcaagg gaatgatggc aatagtttta ttacttttgt aaagcaattg
     3601 tatgatcgca atcgcagtga taatgctatt attttatcta gcgctcatgc agcaaaaggg
     3661 ctagaaagta ataatgttta tattctaaat cctcatcaat ttatgagttt tagacgtgaa
     3721 ccaacagttg aagagtatgt aagcgaagcg aatgttttat ttgttgcttt gtcaagaagt
     3781 aagagcaact taaattttat tggtgaaaaa ccaatagcag ttaaagagca atatcttaat
     3841 tattattttt aaaaggataa tatgaataca ttaactgtaa aacaattaaa atcagagcaa
     3901 tttaaaagtg ttgtatatca aattagtgaa gcaattaaac taataggcga tgaagcgatt
     3961 tattatttac catcagaaga accatatatt agtttggtga tgaaattaga agaatttaaa
     4021 aatattgatt ttgataagat tgattatatc aaagttaatt ggttaacact agctgaatac
     4081 ttgattgatg tcctattaga tggtaaaatc attgatgaag aattagaaat ggaaattaat
     4141 tctttacgtg aagagaatag ttatttaata ggagaaattc atgaattgga tcaaggacaa
     4201 gattttagac tttgcgattg ataggattga aaatatagtt gcaggattaa ttgatttggc
     4261 aattgaaact gttaaagata ataggacaga ggaatttctg atgaaaatca aaaatccttt
     4321 acacaagaaa attgcaattc tattgattga tatcattagg acaacattag aaagtgctga
     4381 taagacaccc gacgtaacag aataagaaat aagcagagat aatctctgct tatttcttat
     4441 tctgttacgt cgggtgtctt atcattagga caacattaga aagtgctgat aagacacccg
     4501 acgtaacaga ataagaaata agcagagata atctctgctt attttattct aaatcaaatc
     4561 gattgcatct tggcttaatt tattattcct taatttggtt aatagaaggt caggtgcaaa
     4621 atcttttaaa aatgaaacag catcatttaa atcaacttct tctttatttt catctaaatc
     4681 acctgtatta agttctaagt cacgagttga tctatcaagg atgcacatta ataaaagcca
     4741 ataattataa tgtaaatcat ctcttgtaat tcctttattt ttatattctt tatctaaact
     4801 ttcttggtat agcttattga agtcttcagg agagaaccag agagaaccag aggaaatgat
     4861 gtagagaata atttctacat actcataatc tatgaacttg tgtaagtatc catagattaa
     4921 taattgaaac aatgcatgtg accatttctt agcttttaac ctatttgcga tagagattga
     4981 cgttaaaatt ccttcttttt gcgcccatct atagaaatta gctcgtttca gaagtttatg
     5041 attttctatt ccagaaagaa aataatcaca tattttcttt aagtttgttg gattgagata
     5101 acattgtctt tggtgtaaag aaatgtgatg aatttctaat tcatcataac aatatataca
     5161 tttttgaaat ttagttgaca tatagaaata ttatagtact tttttattag tttgtcaata
     5221 gaaatttgac aaatataaaa aatgtgatat aattcatttc ataaattaaa ctaagttaaa
     5281 agaaaggcta aatgtataaa agacaatgtt ttattcagaa ctaccaacac ataaatttaa
     5341 taatacaaaa ttaactacaa attttcacaa ccttttcccc gaaggctttt atctaacagc
     5401 aaaggataga ccaagtgcat cttctgcaat gtgtcaacgt caggtagctt tgcagttaac
     5461 aattgataaa acttttgtaa agcaacctaa agcactaaat gactacgcca ggatcggcaa
     5521 tgcgattgaa gatggtgttc tatctcgtta tcgtaaagca aatcagttat ttcttagtca
     5581 atggaaaatt ccagaagcat taacttcgtt aaattttaat ataggtggaa ttattgatgc
     5641 attcttacag attgaaaata cactaatttt gcttgatatt aaatcagttg gttcagtaga
     5701 aagtgaacca acaatttcac tatcatcaaa tgaagttgca atgttaatgc aaggtgagcc
     5761 acttctattt catcctgacg ataatagaat gaaaaattca gttgagaagg ggataaagga
     5821 agctcattta agtcagttgc aactttatgc tgctgtgaca gggtttgatg aagtatacat
     5881 tcaactaatg tcacgaagag ttcaagataa gtttgaattc gataatacag caccaacaac
     5941 aaaatttgaa caaattccag tcgatatttc aacattggaa cgaagagttg caattgtaat
     6001 gtatggaatt gaatgtaaga aaagaggcta tattccagaa cctttgaatg gaattaaaaa
     6061 atctcattgt gcagatgcat tttgttcttt tcaagatttt tgttggaaag ataagacgtt
     6121 aagtttgaaa gaaaatttac agcatattcc tgaaaaagtt ggcagtgatt tgaagaaaga
     6181 atgcattgac caggcaaagg aatatatttc aaaacgtaat gaacgatatg aattaaccct
     6241 ttctcttatc acagatgaaa gaaatagaag attaaagtca aaggttgaac tataatttat
     6301 gaaagtttat ttagatatag atggagttat ttctgatttt tctgctggtt taacgaaaat
     6361 atatccagaa ttcattaatc atgaccaaac atcatacgat ttaccatctt atgttgacct
     6421 tgatacaatc tacaatgata agacattttg gatgttttta cctgtacaag acagaccaac
     6481 agttgaaatt aatggcttag tgtcgcatag accatttgaa aaatatatta cagaattttg
     6541 gttgcatatt aacaagtttc ctaaaattcc tgtatatcat gttaaaagta gtcatgaaaa
     6601 agctgaatta ctaattaatt taaaatgtga tttgtacgtt gacgataagg tatcaacatt
     6661 ttatgaatgt aatagttcag ggataagaac attgctttac aatcaaccct ggaataggaa
     6721 tgtaaaaact gatatgagaa ttaataaatt aaaggagtta gaaaaatggt tgcaatctta
     6781 gctggtgaaa aagtattata tgtgtattac tacaacactg aacaagtttt tgataaatta
     6841 ccggaagcag atttatacct tcctcttgaa gacaatattg caaaacatat tattaaagaa
     6901 acaatgtggg ggaaatattt attggaagtt ctagtaacat ggaatactga ctacccaatt
     6961 tacacaccta ttgctttaca agttgcgcat acatcacttg gaattgtagg agaaattgct
     7021 gaaatccttg gaaccagcga gactgctatt caaacgcagt tgc
//
LOCUS       DAOQVQ010000075         6919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12063_length_6919_cov_4.329254, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000075 DAOQVQ010000000
VERSION     DAOQVQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_6919_cov_4.329254"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1039)
                     /gene="rimK"
                     /locus_tag="PKN22_03620"
     CDS             complement(158..1039)
                     /gene="rimK"
                     /locus_tag="PKN22_03620"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HNS41825.1"
                     /translation="MKIAILSRNANLYSTKRLVEAAELRGHEAHVIDHLRCNIEIEQT
                     GPKIYYGGKYLTDFDAVIPRIGASVTFYGTAVVRQFEMMNVFSVSSSRGIVNSRDKLR
                     CLQVLSREGLGLPKTVFTNYSKDVHHVVQSAGGAPLVLKLLEGTQGLGVVLAENKNAA
                     QSVLEAFNGLKARVIAQEFIPEAKGADIRAFVVDGQIVGAMKRQGKEGEFRSNLHRGG
                     SSVVMELTPEEQATAILASKSVGLAVAGVDMLQSKRGPLVLEVNSSPGLQGIETTTKV
                     DVAGKIIEYIERNVVTR"
     gene            complement(1058..4585)
                     /gene="ccsA"
                     /locus_tag="PKN22_03625"
     CDS             complement(1058..4585)
                     /gene="ccsA"
                     /locus_tag="PKN22_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNS41826.1"
                     /translation="MDKLLRGFFSMKMMTIGLLVFLFGIAVATFLESIYDIQTARLVI
                     YNALWFELLLVYLTINLIINTFRYDMWKKEKIAMLLFHISFVIIMIGAGITRYISFDG
                     IMKLPEPDPQTGMLKPVDYIYSADPRIKVVIGSKYVEKTMYMSEQVKSRFNVDFKFPE
                     KNTTINVELVKFESNRIDSLVVNDSIKDYVLNIVTSGNQSNYLKEGNFIMAGNVPISL
                     EKKDALPGGIELFNQNGRIYVKSGIPLKALPMAMMQQVRQTGQDVPDSMYTHIPADTL
                     VPFNMTTLYYAADQMFVFKEMVQHAKVMRMPASKKKTGSDYITLKFTDGKTTKLVTVE
                     GGMGQITAGERFSFGDLLCNVQYGSVPIQLPFAVACNDFTLHRYPGSDSPSSYESIVS
                     VMDKNNKVLRKQKIFMNNIMDYDGYRFFQSSYYPDETGTVLSVNHDWWGTTITYLGYF
                     IMSLGFVMSIFAKGGRFQELNGKINKIATKRAKAGLSALLILLSTVSYAQTDSVQAIA
                     EENHSHGVEAIAEEHDHQHDHQHDHQHDSTHNHAAEQTSEVPAPDKNAVATYINKEHA
                     DKLAYLMVQDYDGRIVPFHTLCNKLLRKINRHETYQDGDNSYNAVQTILSMHMTPDAW
                     LYKPIIYVPRVLRETLGIKGSSATFMELLNEQEDAFKLADEYEQAHRKLEKERSEFDK
                     QLIKLVEKFQVFSEIQMWQYMKIVPVRNDAKNTWFLPFSQEVSKNEKELFNLTMKYFQ
                     AVSKGIATNNFTDADTQLDALIKVQREVAGNVAPSESKINVEVAYNKMNFFDNSKNLY
                     ILAGLVLLVIFFVQLFSKKGNKDSKATKIIKIISIAVILITFIYQGVGLGMHWYISGH
                     APWSDGYGAIVFISWVSILAGFIFAKKSGVILPATCLLAFFMLFVAGMNILDPEITPL
                     IPVLKSYWLMIHVAIITGSYAFLGLGAILAIINLFLYIFRNKENGKVLTWNINELTYV
                     SEMSQTVGIIMLTIGTFLGGVWANESWGRYWAWDPKETWALVSILIYAITLHLRFIPA
                     MKSKFIFNVFSLWSYAAILFTFFGVNFYLVGLHSYAQGDGLGTFPFEIILATVLFYAF
                     TEFASLKNRRYSKQASEINLNYFVKKTVVTIAVFVLCYLAFIIFKVITINEALEVSWQ
                     TAAVVIVTNALMYAYQIAVPNKPVKLIED"
     gene            4798..6786
                     /gene="uvrB"
                     /locus_tag="PKN22_03630"
     CDS             4798..6786
                     /gene="uvrB"
                     /locus_tag="PKN22_03630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNS41827.1"
                     /translation="MDFQLISEYQPTGDQPEAIAEIVAGLDSGRDFQTLLGVTGSGKT
                     FTVANVIEKINKPTLILSHNKTLAAQLYGEFKQFFPNNAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLQINDEIEKLRLAATSALLSGRQDVIVVSSVSCIYGIGNPREFEKSIIHL
                     QVGETVARNKFLYKLVENLYSRAHEEFKRGTFRVKGDTVDIYLAYADYAIRIEFWGDE
                     VESISSIDPENNSKLDTFQTIAIYPANIFVSSPENTKQALDQIGEDMVVQVAAFKKAG
                     KLDEAKRLEERVSYDMEMIRELGYCSGIENYSRYFDRRQPGERPFCLLDYFPDDYLMV
                     IDESHVTIPQVKGMYGGDRSRKTSLVEYGFRLQAAIDNRPLKFEEFEGMQNQVVYISA
                     TPAEYELEKSEGIVVEQVIRPTGLLDPIIEVRPTANQIDDLIEEIQKRVELDERVLIT
                     TLTKRMAEELQKYFDKLSIRCRYIHSDVDTLERIEILHQLRNGVFDVLIGVNLLREGL
                     DLPEVSLVVILDADKEGFLRNVRSLVQTVGRAARNVNGYVIMYADKMTDSMQKTIDET
                     ARRRRIQMDYNEKHGKSPQPLVKSKEKMIVIGSKAKNEVGDELPMVAEKPVEYSNKEE
                     LKKQIAATKKAMEKAAKELDFIEAARLRDILFELQASI"
BASE COUNT         2176 a         1302 c         1253 g         2188 t
ORIGIN      
        1 taaaacgatg gtgggaccgt cttcttttgc tctatctacg tgaatgggga tttgaaccgg
       61 cgtactggtg tgcaattttg ccacattcaa attcaattca tagtgttttc cgggagcaat
      121 ttcttgaccc aaaatcacta cttttttgta tttgtgttta tcgtgtaacg acattgcgct
      181 caatgtattc gataattttt ccagctacat ccacctttgt tgtggtttca attccttgca
      241 acccaggtga tgaattgact tctaaaacca atggtcctct tttagattgc aacatatcca
      301 cacctgcaac ggcaagtcca acacttttag atgctaaaat agcagttgct tgctcttctg
      361 gcgttaattc cataacaacc gaacttcctc ctcggtgcaa gttggaacga aattcacctt
      421 cttttccttg acgtttcatt gctccaacaa tttgaccatc caccacaaac gcacggatgt
      481 cagctccttt tgcttcgggg ataaattctt gagcaatcac gcgggctttc aaaccgttaa
      541 aggcttctag taccgattga gcagcatttt tgttttctgc caatacaaca cccaaacctt
      601 gtgttccttc caataatttc aacacaagtg gtgctccacc agccgattga actacgtggt
      661 gcacatcttt agaatagttg gtaaaaactg ttttgggtaa tcccaatcct tcacgagaaa
      721 gaacctgtaa acaacgcaat ttatcacgag agttaacaat tcctctagaa ctagaaacag
      781 aaaaaacatt catcatttca aattgacgaa caacagccgt tccgtaaaat gttacagaag
      841 caccaattct aggaattaca gcatcaaaat cagttaaata tttaccgccg taataaattt
      901 taggacctgt ttgttcaatt tctatattgc aacgtaaatg gtcgattaca tgtgcttcat
      961 gtccacgtaa ttcagctgct tcaaccaatc ttttagttga atatagattt gcatttctag
     1021 ataaaattgc aattttcata gtgagaatct aaaaaaatta atcttcaatc aatttaacag
     1081 gtttgttagg aactgctatt tgataagcat acatcaatgc gtttgtaaca atgactactg
     1141 ctgctgtttg ccaagatact tccaaagctt cgtttatagt aattactttg aaaataataa
     1201 acgctaagta acacaaaacg aaaacggcaa ttgtaacgac tgttttcttc acaaaatagt
     1261 tcaaattaat ttctgaagct tgtttagaat aacgtctgtt tttcaacgat gcaaattctg
     1321 taaatgcata gaaaagcacg gttgctaaaa taatttcaaa tgggaaagtt cccaagccat
     1381 caccttgtgc atacgagtgc aatcctacca agtagaagtt aacaccaaag aaagtaaata
     1441 aaattgctgc gtaggaccac aaactgaata cattgaagat aaatttactc ttcattgctg
     1501 gaataaagcg caagtgcaaa gtgatagcat aaattaaaat ggataccaac gcccatgttt
     1561 ctttcgggtc ccaagcccaa tatctacccc atgattcgtt tgcccaaaca cctcctaaga
     1621 atgttccaat agtcagcata ataattccaa ctgtttgcga catttctgat acataggtta
     1681 gctcgttgat attccacgtt aacacttttc cattttcttt gtttctaaat atgtaaagaa
     1741 acaaattgat gatagccaaa attgcaccca aacctaagaa agcataactt cctgtaatga
     1801 tggcaacgtg aatcattaac caatacgatt ttaacacagg aattaacggt gtaatttcag
     1861 ggtcaagaat gttcattcca gccacaaaca acatgaaaaa tgctagtaaa cacgttgctg
     1921 gcaaaataac accacttttc ttagcaaaga taaatccagc taaaatggat acccatgaga
     1981 tgaacacaat tgctccatat ccatcactcc atggtgcgtg tccagaaata taccaatgca
     2041 tacccaaacc aacaccttga tagatgaaag taattaaaat aacagctata cttatgattt
     2101 taatgatttt ggtagcttta gaatctttgt tgcctttttt agaaaacaat tgaacaaaga
     2161 aaattaccaa caacaccaaa ccagccaaga tgtataggtt ttttgagtta tcgaagaaat
     2221 tcattttgtt gtaggcaacc tctacattga ttttagattc agatggtgcc acatttccag
     2281 ccacttcgcg ttgtactttt attaaagcat ctaattgcgt gtcagcatct gtaaaattat
     2341 tcgttgcaat tcccttacta accgcttgga aatatttcat tgttaagttg aataattctt
     2401 tctcgttttt agaaacttct tgtgaaaaag gcaagaacca cgtgtttttt gcatcatttc
     2461 ggacaggtac aattttcatg tattgccaca tttgaatttc agaaaaaact tggaattttt
     2521 ctactaattt aatgagttgc ttatcaaact cacttctttc tttttctaat ttacggtgtg
     2581 cttgctcgta ttcatcagct agtttaaatg catcttcttg ttcgttcaag agttccataa
     2641 aagtagcgct agaacctttt attcccaatg tttctctcaa cactcttgga acataaataa
     2701 ttggtttata caaccaagca tctggtgtca tgtgcatgct caaaatggtt tgaactgcat
     2761 tataggaatt gtcgccatct tggtaagttt catggcgatt aattttgcgc aataatttgt
     2821 tgcaaagtgt gtggaacgga acaatgcgcc cgtcgtaatc ctgcaccatc aaataagcta
     2881 atttatcagc atgttcttta ttgatgtagg ttgcaaccgc gtttttatca ggtgctggca
     2941 cttcacttgt ttgttcagct gcatgattgt gcgtagaatc gtgttgatga tcgtgttgat
     3001 gatcgtgctg atgatcgtgt tcttctgcaa tggcttctac tccgtgagaa tgattttctt
     3061 ctgcaattgc ttgtacacta tctgtttgcg cgtatgaaac ggtgctcaac aagataagca
     3121 aggccgataa tccagctttt gcacgtttgg ttgcaatttt attaattttc ccattcaatt
     3181 cttggaaacg tccacctttt gcaaaaattg acattacaaa cccaagtgac ataatgaaat
     3241 aacctaaata agttatggtt gttccccacc aatcgtggtt aacactcaat acagttcctg
     3301 tttcatcggg ataatacgaa gattggaaga agcggtatcc gtcgtaatcc ataatgttgt
     3361 tcatgaatat tttttgtttt ctaagtactt tgttgttttt atccatcaca gacacgatac
     3421 tttcatacga agaaggtgag tctgagcctg ggtaacggtg caatgtaaaa tcattacaag
     3481 ccacagcaaa tggcagttga attggaacag agccgtattg cacattgcac aacaaatcgc
     3541 caaaagaaaa gcgctctcca gcagtaattt gtcccatacc accttcaaca gtcaccaatt
     3601 ttgtagtttt tccatctgta aacttcaacg taatgtagtc gctacctgtt ttctttttac
     3661 ttgcaggcat gcgcattact tttgcgtgtt gcaccatttc tttaaacaca aacatttgat
     3721 cggcagcata atacaacgtt gtcatgttaa aaggtaccaa tgtatcagca ggaatgtggg
     3781 tgtacattga atctggaaca tcttgccctg tttgtcttac ttgttgcatc atcgccatgg
     3841 gtaaggcttt taatgggatg ccagatttta cataaatgcg tccattttga ttaaacaatt
     3901 caattccacc aggcaaagca tccttttttt ctaaagaaat aggcacattt ccagccataa
     3961 taaaatttcc ttctttcaaa taattggatt gattaccact cgtaacaatg tttaacacgt
     4021 aatctttgat gctatcgttt accaccaaac tgtcgatgcg gttggattca aattttacca
     4081 attccacatt aatggttgtg tttttctctg gaaatttgaa gtctacattg aaacgcgatt
     4141 taacttgttc ggacatgtac attgtttttt caacatactt actgccaatc acaactttaa
     4201 tacgtggatc tgccgaatag atgtaatcaa caggttttag catccccgtt tgcggatcgg
     4261 gttctggtaa tttcataatt ccatcaaaac taatgtatcg tgtaattcca gcaccaatca
     4321 tgatgattac aaacgaaata tggaacaaca acatggcaat tttttctttt ttccacatgt
     4381 catatctaaa tgtgttgata atgagattga tggttaaata aaccaataat aactcaaacc
     4441 acaaagcatt ataaattacc aaacgtgcag tttgtatatc atagattgac tctaaaaatg
     4501 tggctacggc aataccaaat aaaaatacga gtaaaccgat tgtcatcatt ttcatggaaa
     4561 aaaatcctct tagcaacttg tccatacttt ttattatttg cccacaaaaa taagcattgt
     4621 aaaaggaaaa accctttttt ttagcagatt tattatttac tttttttgta ctttaatatt
     4681 gtgaaaatca ataggatata acaatgttta acgtttatta tgattttaca cataaaacag
     4741 taaaaatgat ttatgagatg taaattatta ttagtcccat tttattaatt ttacactatg
     4801 gattttcaac tcatatcaga ataccaacca acaggcgatc aacccgaagc aattgctgaa
     4861 attgttgctg gacttgattc gggaagagat tttcaaacct tgttaggtgt aactgggagt
     4921 ggaaaaacgt ttacggtggc caatgtcatt gaaaaaataa ataaaccaac actgatttta
     4981 tcgcacaata aaaccttggc agcacaattg tatggcgagt ttaaacaatt ttttcccaac
     5041 aatgcggtgg aatattttgt atcgtattac gattattatc aaccagaggc gtatttgccg
     5101 gtaacggata cctacatcga aaaagatttg caaatcaatg acgaaattga aaaattgcga
     5161 ttggctgcta cttcggcatt gctttctggt cgacaagatg tgattgtcgt atcctctgtt
     5221 tcgtgcattt atggtattgg aaatccgcgt gagtttgaaa aaagcatcat tcacctgcaa
     5281 gttggagaaa ccgttgcacg caataagttt ttatacaagt tagttgaaaa cctctattct
     5341 cgtgcgcatg aggaatttaa acgtggaact tttcgggtaa aaggtgatac ggtggatatt
     5401 tatttagcct atgccgatta tgccattcgc attgaatttt ggggcgatga agtggagtca
     5461 atttcatcca ttgaccctga aaacaattca aaattagata catttcaaac cattgctatt
     5521 tatccagcca atatttttgt gtcgagtcca gaaaacacca aacaagcact cgatcaaata
     5581 ggtgaggaca tggtggtgca agtggcagcg tttaaaaaag ctggaaaatt ggatgaagca
     5641 aaacgattag aagaacgtgt ttcgtatgac atggagatga ttcgtgagtt gggctattgt
     5701 tcgggaattg agaattactc acgctatttt gacagacgac aaccgggtga gcgccctttc
     5761 tgtttgttgg attatttccc tgacgattat ttgatggtca ttgatgaaag tcacgttacc
     5821 attccgcagg tgaaaggtat gtatggtggc gaccgttctc gtaaaactag tttagtagaa
     5881 tatggttttc gtttgcaagc tgctattgat aatcgacctt tgaaatttga agaatttgag
     5941 gggatgcaaa atcaagtggt gtacatttct gctactccag ctgaatatga attggaaaaa
     6001 tcggagggaa ttgtggtgga gcaagtcata cgtccaaccg gattattaga tccaatcatt
     6061 gaagtgcgcc caactgccaa tcaaattgat gatttaattg aagaaattca aaaaagagtt
     6121 gaactggatg aacgtgtttt gataacaacg ttgaccaaac gtatggcaga ggaattgcaa
     6181 aaatattttg ataagctttc cattcgttgt cgctacattc attcagatgt agatactttg
     6241 gaacgcattg aaattctgca ccaattgcgc aatggtgttt ttgatgtgtt gataggtgtt
     6301 aatttattgc gagagggatt ggacttaccc gaagtgagtt tggtggttat tttagatgct
     6361 gacaaagaag gttttttgag aaatgttcgt tcgttggtgc aaaccgttgg tagagctgca
     6421 cgcaatgtga acggctacgt catcatgtat gccgataaaa tgaccgattc catgcaaaaa
     6481 accattgatg aaactgcaag aagacgacga attcagatgg actacaatga aaaacacgga
     6541 aaatcgcctc agccacttgt gaaatccaaa gaaaaaatga ttgttatcgg ttctaaagcc
     6601 aaaaatgaag tgggagatga actgccaatg gtcgccgaaa agccagttga atattccaat
     6661 aaagaagagt tgaaaaaaca aattgcagcc accaaaaaag caatggaaaa agcagccaaa
     6721 gaattggatt ttattgaagc agctcgttta cgcgatattt tatttgaatt gcaagcttcg
     6781 atttaacaag gattgcgtaa agattcctct cctaataatt tgcttggttt agggatgcaa
     6841 agtttacctc ctttgtgatt tttaacatag ttaatgatat aatcaaacgc ctcttgcaca
     6901 tcgtcggtaa acaaaaaca
//
LOCUS       DAOQVQ010000076         6809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12301_length_6809_cov_3.831211, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000076 DAOQVQ010000000
VERSION     DAOQVQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12301_length_6809_cov_3.831211"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKN22_03635"
     CDS             <1..399
                     /locus_tag="PKN22_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41828.1"
                     /translation="KNVTSLNVGKNKLKNLNGIEQLEHLTYLNAEKNILEYFPNEICQ
                     LKQLETLILNRNIYIDAVPACIENCQQLKTLDLWYTAVDHLPIEMTTIKTLKLIDFSG
                     VQINQEGQNKLKKMFPNTKLVLSAPCNCMN"
     gene            501..1118
                     /locus_tag="PKN22_03640"
     CDS             501..1118
                     /locus_tag="PKN22_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNS41829.1"
                     /translation="MFLLLFVAMSKINAQQQIDTTKLIQLSGVVVSETELNTMPYTTI
                     YDKTQKRGVMADYYGYFSLVVLPSDTLYFSYYGYKTSMFVVPDTLTENRYSIIHMLEK
                     DTVNLPEVTVYPWPSKEDFARYFVEMNPTDDAIKRAQRELSGESLAFAAARVKSDASL
                     AHGVVQNQMNTKIYTRGQIPGNNLLNPFAWSKFVKDWKEGKLKRQ"
     gene            1128..2027
                     /gene="menA"
                     /locus_tag="PKN22_03645"
     CDS             1128..2027
                     /gene="menA"
                     /locus_tag="PKN22_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNS41830.1"
                     /translation="MTKKQAWIVAIRPRTLPLSLSGIIFGAAIAFQQGFIDWTIFSLA
                     ILTTLLFQILSNLANDYGDGVKGTDNEERVGPTRAIQSGVISQKEMKSAVVFTSILSF
                     VSACLLIYFGTQQMPLATIWYYVMLAILCIIAAITYTVGKKAYGYHGLGDVMVFIFFG
                     LVSVLGVYPLFAKQFDWNNIYPAITIGLLSTAVLNLNNMRDYFSDKNAQKNTLVVKMG
                     INNAKIYHTLLIIIALVVLVFFIQKSNQVISYIALLPAVSLLLHVYKVAKVQDTKAFD
                     PELKVVALSTFAIAVLYFISIWL"
     gene            2029..3054
                     /locus_tag="PKN22_03650"
     CDS             2029..3054
                     /locus_tag="PKN22_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNS41831.1"
                     /translation="MKARFFYSPLEFKRPAGTSRGVLNIKHSWILEIEKNGVVGTGEC
                     SIIPDLSPDYQSNDQYEAVLKNVCSQIQSGELTFENASQLLKDFPSLLFGVECAFLDL
                     QNGGHKIYFDNDFSKGNQKIPINGLIWMGDEVFMQEQIEQKLAAGFTTIKMKIGAIDF
                     DTEYRLLKSIRQQFDQQTITLRVDANGAFSPQEAKNVLELLAQLDIHSIEQPIKAGQW
                     QVMRELCNSAPTPIALDEELIGINEKQQKIELLTSIQPQFIILKPSLHGGISGTKEWI
                     QLAEEQNIAWWMTSALESNIGLKAICELTAEYNNQLPQGLGTGSLYVKNHETSLIVEQ
                     GMIYWKS"
     gene            complement(3058..3939)
                     /locus_tag="PKN22_03655"
     CDS             complement(3058..3939)
                     /locus_tag="PKN22_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41832.1"
                     /translation="MNNRLQIVERANINEEKWNQLLQRSSASVYNQLYFLDSLSEHWC
                     ALIYDDYKGAIALPYTIRLGVKGLFTPNFIRSLSWLGDLPNDFNDVEIKLKNEFKRAN
                     YHIDQLLFKESTKRVYQLIKSSEQIKIGSQTKRALKKFEKSGMIIQQVEISRGIDLIV
                     KELQNKVKNLREIDFVRFKQLLLHYNLSTISCWTAQKNDSIHASLILIEWNNEVLYIK
                     GGVDEIGKKEGAMHGLMHQVIQTTLKHNKIFSFEGSTIESVRQFNCGFGAADSVYYMV
                     KWDNSPWWFKLLNKLRK"
     gene            3960..4442
                     /locus_tag="PKN22_03660"
     CDS             3960..4442
                     /locus_tag="PKN22_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNS41833.1"
                     /translation="MSIKLPYFFYMEIERKFLVNEQLWKEHKPLLGKSIKQGYFLSNE
                     KISLRVRTKGEKGYITIKGATVGISRDEYEYEIPYSDALELLEKYAQPYLEKTRYEVQ
                     WEGKTWEIDEFHGKLAPLILAEIELKSEDDYFELPPWVTEEVSENPAYFNSEIIKKLG
                     "
     gene            complement(4504..>6809)
                     /locus_tag="PKN22_03665"
     CDS             complement(4504..>6809)
                     /locus_tag="PKN22_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41834.1"
                     /translation="WQNLTLNATATSDFIASIPTGWFGLGLSTTGIASGHYDFLWIRA
                     HSHANKPYILVDYPLLTTQPVTTNICTNGSGSFTVGANATSPSYLWQYSTNGGSTWLS
                     TVGVSQCSGQTTNTLTITNPPLGWNNTALVRCAVTSGSCTLYSNAVLFAVKDASTAPT
                     TVTGGGNYCFGNSIALNSSGGTSGDNAVYAWYKGGCNNAFTETWMNLSSTYTFPNTTV
                     NSTTNGILNVTSTNNDPMIHMPNIGSFSPTTYRYVNIRYRVVSGTANSAEIFFYNAHY
                     PSANAAHQTSTSLISDGNWHIATIDLTQNSHYLGDGSNITGWRYDWATNAGCTMELDF
                     IQLSQYPMIDEDNTTTALEWTPAHPHYPGAGTTTYATTRIDNCGATSCASTTVTLPSK
                     TNVLATDGESATCTVNANETVHFYNTTSGRYIASVSAGATGLGSTVATTYVAGSPIIT
                     TECGNPTLETAVLARHWVINPTTNVGATVRLPYYNSELTALYPASTATTNPIDDISAQ
                     AQLGLSKYSGPLNVNSSWADNCPPSGNGNTTYQMPNGFADLSTYSPLSWPANGDKYSW
                     FVISGFSEFWLHGSQNLSPLAVVLSDFSTTCADEYTHIRWTTASEQNSDYFTVEYSRD
                     GSQWKTIETVDGAGTINTSQQYQITTLSKDEGYYRLKQTDTDGTTKTLETVFSACKST
                     SNSMNVYPNPSNGTFTLQLNSSEELENAVITIQELSGRIVEQRWVNVKMGTNSIYFEN
                     NQLNAGSYIISVNGKESAFSPLRMVIQ"
BASE COUNT         2205 a         1215 c         1229 g         2160 t
ORIGIN      
        1 aaaaacgtca cttcgttgaa tgttggtaaa aacaaactaa aaaatttaaa cggcatagag
       61 caattggagc atttaaccta tttgaatgcc gaaaaaaaca tattggagta ttttccaaat
      121 gaaatctgcc aattaaagca acttgaaacc ttgattctca atagaaacat ttacatagat
      181 gccgttcctg catgtattga aaattgccaa cagttaaaaa cattggattt gtggtacact
      241 gccgttgatc atctcccaat agaaatgaca acaataaaaa cattgaaatt gattgatttt
      301 tcgggagtgc aaattaacca agaaggacaa aacaagttaa aaaaaatgtt tcccaacaca
      361 aaattagtac tgagtgcacc ctgtaattgc atgaattaat caataaacga taaaaaaatg
      421 ttaactttat taaaaaaata atattccgct tgaaaatcaa ttatacttgt tatatgaaaa
      481 acttaaaaaa tagtgtgtta gtgtttcttt tactttttgt agcaatgagt aaaatcaatg
      541 ctcaacaaca gattgacact acaaaattaa ttcaactatc tggcgtagta gtttctgaaa
      601 cagagttgaa taccatgcct tacaccacca tttatgataa aacacaaaaa agaggggtga
      661 tggctgatta ctatggttat ttttcgttgg ttgttctacc aagtgatacg ttgtatttct
      721 cttattacgg ttataaaaca tcgatgtttg ttgtgccaga tacattaaca gaaaatcgtt
      781 attcaattat tcacatgttg gaaaaagaca cggttaattt acctgaagta accgtttatc
      841 catggccttc aaaagaggat ttcgcccgct attttgtgga aatgaaccca acagatgatg
      901 caatcaagcg tgcacaacgt gagttatcag gagaaagttt agcttttgct gctgctcgtg
      961 ttaaatcgga tgcatcatta gcgcatggcg ttgttcaaaa tcaaatgaat acaaagattt
     1021 ataccagagg acaaattcca ggaaacaacc tgctcaaccc atttgcttgg tctaaatttg
     1081 tgaaagactg gaaagaaggt aagttaaaac gtcaataaac aaattacatg accaaaaaac
     1141 aagcttggat agtagctatt cgtccacgaa ccttgccttt aagtttgtcg ggaataattt
     1201 ttggagctgc cattgcattt caacagggtt ttattgattg gacaattttt tcattggcca
     1261 ttttaactac gttgttgttt caaattcttt ccaatttggc aaacgattat ggtgatggcg
     1321 tgaagggaac cgacaatgaa gaacgtgttg gtccaacaag agccatacaa tcgggtgtta
     1381 tttcgcaaaa agaaatgaaa tctgccgttg ttttcacctc cattttaagt tttgtatctg
     1441 cttgtttgct tatttatttt ggaacgcaac aaatgcctct tgccaccatt tggtattacg
     1501 taatgttggc tattttgtgc atcattgctg caattaccta cactgttggt aaaaaagctt
     1561 atggctatca cggattgggc gatgtgatgg tgtttatttt ctttggattg gtgagtgttc
     1621 tgggtgttta tccgcttttt gcaaaacaat ttgattggaa caacatttat cctgccatta
     1681 caattggctt gttgagtacc gctgtattga atttaaacaa catgcgtgat tatttctcag
     1741 acaaaaacgc acaaaagaat acgttagtag tgaaaatggg aattaataat gctaaaattt
     1801 atcatacgct gctgattatt attgctttgg tagtgttggt gtttttcatt caaaagagca
     1861 atcaagtaat aagctatatt gcattattac cagctgtttc cttgttatta cacgtataca
     1921 aagtagcaaa ggttcaagac accaaagcgt ttgatcctga attaaaagta gttgctcttt
     1981 ccacttttgc aatcgctgtt ctttatttca tctccatttg gttgtaaaat gaaagctcgt
     2041 tttttctatt ctcctcttga atttaaacgt ccagccggaa caagtagggg cgttttgaac
     2101 attaaacatt cgtggatact agaaattgaa aaaaacggtg tagttggaac aggtgaatgc
     2161 agcatcatcc ccgatttatc tcccgattac caatccaacg atcaatatga agctgtatta
     2221 aaaaatgttt gctcacaaat tcaatcgggt gaattgacat ttgaaaacgc atcgcaacta
     2281 ttaaaagatt ttccttcact gttatttgga gttgaatgtg catttttaga ccttcaaaat
     2341 ggagggcata aaatctattt tgacaacgat ttttcgaaag gaaatcagaa aatacccatc
     2401 aacgggttga tttggatggg tgatgaagtc tttatgcaag agcaaataga acaaaaattg
     2461 gctgctggtt tcacaaccat taaaatgaaa attggcgcaa ttgattttga caccgaatac
     2521 cgattattga aatctatacg ccaacaattt gatcaacaaa ccatcacctt gcgtgtggat
     2581 gccaacggag ctttttctcc gcaagaagca aaaaatgtat tggaactttt ggcgcaacta
     2641 gacattcatt ccattgagca acctatcaaa gccggacaat ggcaagtaat gcgtgaattg
     2701 tgtaactccg ctcctactcc aattgcttta gacgaagaat taattggcat caacgaaaag
     2761 caacaaaaaa ttgaactttt aacaagcatt caaccgcaat tcattattct aaaaccaagt
     2821 ttacacggtg gaatttcggg tacaaaagag tggatacaac ttgccgaaga acaaaatatt
     2881 gcttggtgga tgacgagtgc cttggaatcc aacattggtt tgaaagccat ttgtgaacta
     2941 accgcagaat acaacaacca actcccgcaa gggttgggaa ctggaagttt gtatgtgaaa
     3001 aatcacgaaa caagcctaat tgtggaacaa ggaatgattt attggaaatc ttaaatgcta
     3061 ctttctgagt ttgtttagta gtttaaacca ccacggacta ttatcccatt tcaccatata
     3121 ataaacagaa tcagcggctc caaatccaca attaaattgg cgtacagact caattgtaga
     3181 tccttcaaaa ctaaaaattt tattgtgctt caatgttgtt tgaatgactt gatgcattaa
     3241 accatgcata gcaccttctt tttttccaat ttcatcaaca ccacctttta tgtataacac
     3301 ttcgttattc cattcaatta aaattaatga agcatgaata ctatcatttt tttgtgcggt
     3361 ccaacaagaa atggtagaaa gattataatg aagtagcaat tgtttaaatc gaacaaagtc
     3421 aatttcacgc aaatttttca ctttattttg aagttcttta acaattaaat ctattccacg
     3481 gctaatttca acttgctgaa taatcattcc agatttttca aactttttca aagctctttt
     3541 tgtttgcgaa cctattttaa tttgctcact acttttaatt agctgataaa cacgttttgt
     3601 actctcttta aaaagcagtt gatcaatatg atagttagct cgtttaaatt cattttttag
     3661 ttttatttca acatcattaa agtcatttgg caaatcgcct aaccaagata gtgaacgaat
     3721 gaaattgggt gtaaataaac ctttaactcc caaacgaatg gtatagggca aagcaattgc
     3781 tcctttgtaa tcatcataaa ttaatgcaca ccaatgttct gaaagtgaat ctaaaaagta
     3841 taattgatta taaacagaag ctgaagaacg ttgcaacaat tggttccatt tctcttcatt
     3901 tatgttagca cgttcaacta tttgcaaacg attattcata tctgtttatt ttagtaactt
     3961 tgagtataaa attaccttat tttttttaca tggaaataga acgcaaattt ttagtcaacg
     4021 agcaactttg gaaggaacat aaacctttgc taggaaaatc catcaaacag ggttattttt
     4081 tatcgaatga aaaaattagc cttcgtgttc gcacaaaagg tgaaaaaggc tacattacca
     4141 taaaaggtgc aacggttggt atttctcgtg acgaatacga gtatgaaatc ccctactccg
     4201 atgcgttgga attattggaa aaatatgccc agccttattt agaaaaaaca cgctacgaag
     4261 ttcaatggga ggggaaaacc tgggaaattg atgaatttca cggaaaatta gcgcctttga
     4321 ttcttgccga aattgaatta aaatcagaag acgactactt tgaattacca ccttgggtaa
     4381 cagaagaagt aagtgaaaat ccagcttatt tcaattcaga aattatcaaa aaattgggat
     4441 aaaaaaaaga agagtcagct ttatgccgac tcctctaacc taaactatat actacaattt
     4501 ttactattga ataaccattc gtaatggcga aaaagcggat tcttttccat ttaccgaaat
     4561 gatgtaggaa cctgcattca attgattgtt ttcaaaataa attgaatttg ttcccatttt
     4621 tacatttacc catctttgtt caacaattct accgcttaac tcttgaattg taataacggc
     4681 attttctaac tcttcacttg aatttaattg aagtgtaaat gttccgttgc ttggatttgg
     4741 ataaacattc attgaattgg atgtggattt acatgcacta aaaacagttt ctaatgtttt
     4801 agttgttcca tccgtatctg tttgttttaa acgataatat ccttcatctt ttgataatgt
     4861 tgtaatttga tattgttggg aggtatttat tgttcctgca ccatcaacgg tttcaattgt
     4921 tttccattga cttccatcac ggctgtattc aactgtgaag taatctgaat tttgttcaga
     4981 tgcggttgtc caacgaatgt gtgtgtattc atcagcacaa gtggttgaaa aatcggataa
     5041 aacaactgcc aacggtgaca agttttgaga tccatgcaac caaaattctg agaaaccaga
     5101 aatgacaaac catgaatatt tatctccatt tgctggccat gacaatggtg aataagttga
     5161 taaatcagca aaaccatttg gcatttggta agttgtattg ccatttccac taggcggaca
     5221 gttatctgcc caagaactgt tgacgtttaa aggtcctgaa tatttactta accccaattg
     5281 tgcttgtgca gaaatatcat caataggatt agtagttgca gtacttgctg ggtacaacgc
     5341 cgttaactct gaattgtaat acggcaagcg aactgttgca cccacatttg ttgttggatt
     5401 aataacccaa tgacgagcca aaacagctgt ttccaatgtt gggtttccac attctgtggt
     5461 aataattggc gaacctgcta catacgttgt tgcaacagtg cttccaagtc cagttgcacc
     5521 agcagaaaca gatgcaatgt atcttccact tgttgtattg taaaaatgca ccgtttcatt
     5581 ggcgtttaca gtacaagtag ctgattctcc gtctgtggca agcacattag ttttactagg
     5641 taatgttaca gtagttgaag cacaacttgt tgccccacaa ttatcaattc tagttgttgc
     5701 atacgttgtt gtaccagccc caggataatg aggatgagca ggcgtccact ccaaagccgt
     5761 tgttgtattg tcttcatcta tcattgggta ttggctcaat tgaatgaaat ccaattccat
     5821 tgtacaacca gcatttgttg cccaatcgta gcgccaacct gttatattgc ttccatcccc
     5881 taggtagtgc gaattttgtg ttaaatcaat agttgcaatg tgccaattgc catcactgat
     5941 aagcgatgtt gatgtttggt gtgcagcatt tgcagatgga taatgtgcat tgtaaaaaaa
     6001 tatttctgca gaatttgcag tacccgaaac cacacgataa cgaatgttaa cgtatcgata
     6061 tgtagttgga ctaaaagacc caatatttgg catgtgaatc attgggtcgt tattcgtaga
     6121 tgttacattt aatattccgt tagtagttga gttcacagta gtatttggaa aagtataagt
     6181 tgaactgagg ttcatccacg tctctgtgaa tgcattgtta caacctcctt tataccacgc
     6241 ataaactgca ttgtcaccac ttgtaccacc tgatgaattc aatgcaattg aatttccaaa
     6301 gcaatagttg ccaccaccag taactgttgt tggagccgta gatgcatctt taaccgcaaa
     6361 aagaacagca ttggaataca aggtacaact accagatgta acagcacaac gaactaatgc
     6421 ggtgttattc catcccaaag gaggatttgt aattgttaac gtattggttg tttgtcccga
     6481 gcattgcgat actcctacag tagagagcca tgttgaccct ccatttgttg aatattgcca
     6541 caaataactt ggtgatgtag cattagcacc tacggtaaaa gatcccgaac cattagtaca
     6601 aatattggtt gttacaggtt gagtagttaa caatggataa tctactaaaa tataaggttt
     6661 attggcatgg ctgtgtgcac gaatccataa gaaatcatag tgacctgatg caatccctgt
     6721 agtgcttaat cctaaaccga accaaccagt aggaatgctt gcaataaaat cggaagtggc
     6781 agtagcgttt aatgttaaat tttgccatc
//
LOCUS       DAOQVQ010000077         6808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12307_length_6808_cov_4.046794, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000077 DAOQVQ010000000
VERSION     DAOQVQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12307_length_6808_cov_4.046794"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKN22_03670"
     CDS             complement(<1..932)
                     /locus_tag="PKN22_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41835.1"
                     /translation="MRRLLIFCAVAVSLTVNGQTTEKYNSVYAGFYKAEELFQKQQYG
                     SARKEFRDFINSFQSTNDPLYIKALYYEGVSAIELFNNDGERLLKQFLLNYPETIYAT
                     AIHFKLGNYYYRAKDYKEALAYYDKIQVKDLQASEKDEYHFKVGYAYFQLDNLKQARN
                     NFAEVKDGDSQYAVPALYYFSHISYTEKSYQTALDGFLKLENDPGFGKVVPYYILQIY
                     YLQGKYEQVAEYAPKLENATIVNEKDINHLVGDAYYRVGKFDEAVPYLQKYDKLANTT
                     RDEDYQLGYALYKTRSFDNAVKLFDRADKDTTDG"
     gene            1092..1802
                     /locus_tag="PKN22_03675"
     CDS             1092..1802
                     /locus_tag="PKN22_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS41836.1"
                     /translation="MEQVIRIKYGRILQGENQVIRNINFEMKGDELVYLIGKTGSGKS
                     SLLKVLYGELPLHEGEGEVAGFDLSSLKKKQVPLLRRHLGIVFQDFQLLSDRTVGDNL
                     RFVMGATGWTEKHLMDKRAMEVLQLVGLEKKINNKPHTLSGGEQQRIAIARALLNKPN
                     LILADEPTGNLDPETSEEIMQLLIAVAKEEKSAVLMATHDMTIVEKFPGRVIKVEDGL
                     LKEVNSMNRFNPFETMNL"
     gene            complement(1858..3981)
                     /locus_tag="PKN22_03680"
     CDS             complement(1858..3981)
                     /locus_tag="PKN22_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNS41837.1"
                     /translation="MNSSFDKQTLKDLEFPTIQQWLEKYAVGPTAKARINQLEPSNDF
                     KAVETELLKSNELLAIRKTGEQFPSLDFEELTQEIKLLAIKNAVLSHEGFYTIRLASN
                     VANQLVYFFNKREKDYPLLAATVEQVEYTKDIIEKIDAIFDKSWKVVDAASPLLAEIR
                     SEIKLLRQQINKNFDKEMRRLAKDNILGDTHEAFYNERRVLTVLASHKRSVAGNIVGS
                     SKTGSLVYVEPQINVELNNELELQIDEERKEIYRILKVLTAEIAVYLPLIEAYQQVLT
                     EFDFINAKTRLALELNCHLPGINRSMHIDLMEAFHPILWKSNKLVGKKTFPQHIQLHN
                     KARMLVISGPNAGGKSITLKTIGLLQVMLQAGILIPVHPNSKICFFQQVLSDIGDNQS
                     IENELSTYSYRLRRMKYFLKVTNRRTLLLLDEFGTGSDPELGGALAEVFFEELYKKEC
                     FAVITTHYANIKLKADALPQAVNGCMLFDTDTLEPLYQFSVGQPGSSFTFEVAQNNGI
                     SQRIIDEAKKRLDDKRVKMDHLLGELQKEKNYFQKLNQEHREAQEAADEARQFFLEKN
                     EHLKQRLKQQQELSDKNNKWLNYGKKIQSFIDRFNTVSRKKDVNQALLDELRQFIALE
                     KGKTEDSKRIEKLKAAVAKPKKKKKPAVEKDDYQQAKIVVGSTVKLISTKQSGTVEEV
                     NGNQITVEFGFMRMKVEREKLMWIK"
     gene            4091..4990
                     /locus_tag="PKN22_03685"
     CDS             4091..4990
                     /locus_tag="PKN22_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS41838.1"
                     /translation="MRKLIIACSLLLLGTTANAQKGFYTDINFGYGVCIPNSVIAVDL
                     YKNLDNPDLSYQRNVYGTLGRGFNVSVNPGYMITKHIGVELGINYFIGAESMLDTNKT
                     TSDPNLYDVTKAKSNQLRIIPAVVINTGGSKLYGYAKAGLVLPIAGSTEAVRSASFSV
                     LVDPINHVKQVYSNEFKATVNGSFSLGFKGSLGIGYNFTPNIGLNLEVFCTALQVKAK
                     TRKVVETAKVDGVVVENDPAPTYYTETNYVDNLSSNSNNDKYNPNFDVTKPKDELARK
                     TSFNQFGFNFGITFKVGSKSTKE"
     gene            complement(5157..5819)
                     /locus_tag="PKN22_03690"
     CDS             complement(5157..5819)
                     /locus_tag="PKN22_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNS41839.1"
                     /translation="MKKLLTLFLGLTVAVGSFAQTTEGVVKYTTEFKSSDPQIQSQLA
                     MLNGSTMTTYFTPEFTRVESNMGMFMKTSTIIDLKSKQSLMLMDGMGSKKAIKTNLDE
                     LDKEDGEEAKVEVEKTSKTKKIAGYKCTQYILTTEDGTTMNYWTTTELSGSKAGNKFM
                     NEKVEGYPLEFELANNGVVMKFTATEVAKGIKEDKAKLFNMDIPEGFQEASMDDLKNM
                     GM"
     gene            complement(5929..6153)
                     /locus_tag="PKN22_03695"
     CDS             complement(5929..6153)
                     /locus_tag="PKN22_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNS41840.1"
                     /translation="MGEISANEQLIQLANTKMPFGKYQGYYLIDLPEYYIVWYRNKGF
                     PKGKIGEQLQLIYEIKLNGLESLIRPLIQR"
     gene            6379..>6808
                     /locus_tag="PKN22_03700"
     CDS             6379..>6808
                     /locus_tag="PKN22_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS41841.1"
                     /translation="MKRELAKDIIQWDIKSWSKALRYWESQIDWGKIKNGLELGGREG
                     GLSLWLALKEKDVVCSDLKDVQKSAEKLHIRHNVTSRIIYQDIDATNIPYENYFDIIV
                     FKSIIGGIGRNDKHENQQQVFKEIYKALKPGGKLLFAENLI"
BASE COUNT         2088 a         1197 c         1223 g         2300 t
ORIGIN      
        1 aatccatcgg ttgtatcttt gtctgctctg tcaaacaatt taacagcatt gtcgaaacta
       61 cgtgttttgt acaaagcata ccccaattgg taatcttcat cgcgggtggt gttggcaagc
      121 ttatcgtatt tttgaagata aggaacagct tcatcgaatt tccctacgcg gtaataagca
      181 tctccaacca ggtgattgat atctttttca ttcacaatgg ttgcattctc caattttggt
      241 gcatattctg ccacttgctc atattttcct tgcaagtaat aaatttgaag aatgtaatac
      301 ggcaccactt ttccaaatcc cgggtcgttt tccaatttta aaaagccatc taaagcagtt
      361 tggtaacttt tttcggtgta ggaaatgtgc gagaaataat acaatgccgg cactgcgtat
      421 tgcgaatcgc catctttaac ttctgcaaaa ttattgcgtg cttgctttaa attatccaat
      481 tgaaaatagg cataacccac tttaaaatgg tattcgtctt tttcagaagc ttgtaaatct
      541 tttacttgta ttttatcgta ataggccaat gcttctttgt aatcttttgc acggtaataa
      601 taatttccta atttaaaatg gatagccgtt gcatagattg tttcaggata attgagcaaa
      661 aactgtttca ataaacgttc gccatcgttg ttaaataatt caattgccga aacaccttcg
      721 taatacaacg ctttgatgta aagcggatca ttggtagatt gaaacgagtt gataaaatcg
      781 cgaaattctt tgcgtgccga gccgtattgt tgtttttgaa acaattcttc tgctttgtaa
      841 aaaccagcgt aaacactgtt gtatttttca gttgtttgtc cgttcaccgt caaactcact
      901 gcaacagcac aaaaaatcaa taatcgacgc atacttaaat tttaatttaa tttgtaaaat
      961 taacccacaa cttggcagga tggggcgcta attttaaaag ttttaaacga agttatgtta
     1021 agattatttc ttactcgttt ttgattcttt gttgaattat taccaatttt acaaaaagaa
     1081 aaaaagcaat cgtggaacaa gtaattcgca ttaaatacgg tagaattctt caaggagaaa
     1141 accaagttat cagaaacatt aattttgaaa tgaagggcga cgaactcgtg tacctcattg
     1201 gtaaaacagg ttctggaaaa agtagtttac tcaaagtttt gtatggtgaa ttgccgttgc
     1261 acgaaggcga aggcgaagtt gctggctttg atctttcatc gttaaaaaag aaacaagttc
     1321 cgctgttgag aagacatttg ggcattgttt ttcaagattt tcaattgcta tctgaccgca
     1381 ccgttggcga caatttacgt tttgtgatgg gagctacagg ttggaccgaa aaacatttga
     1441 tggataaacg tgccatggag gtgttgcaat tagttggatt ggaaaagaaa atcaacaaca
     1501 agccacacac gctatcgggt ggtgagcaac aacgcattgc cattgcacgt gctttattaa
     1561 acaaacctaa tttaatttta gccgatgaac caacaggaaa tttggatcct gaaacgtcgg
     1621 aagaaattat gcaattattg attgccgttg caaaagaaga aaaatcggct gttttgatgg
     1681 ccacacacga catgactatt gtagaaaaat tccctggtcg tgttatcaaa gttgaagatg
     1741 gtttattgaa agaagtaaat tcaatgaatc gcttcaatcc gtttgagacg atgaatttgt
     1801 agaaagtgat tggtgatttt ggttattgag gtgtgtatcg tcctaaaaaa gtttacatta
     1861 ctttatccac atcaattttt cgcgttctac tttcatacgc ataaaaccaa attcaacggt
     1921 aatttgatta ccattgactt cttctaccgt tcctgattgt ttggtagaga tgagtttaac
     1981 ggttgaacca acaacaattt tggcctgttg ataatcgtct ttttctacag ctggtttctt
     2041 tttctttttg ggttttgcaa cagctgcttt gagtttttca atgcgtttac tatcctctgt
     2101 ttttcctttt tcgagggcaa tgaactgtcg aagttcgtct aacaacgctt gattgacatc
     2161 ttttttgcga cttaccgtat tgaaacgatc aataaaactt tgaattttct taccataatt
     2221 gagccatttg ttgtttttat cgctcaattc ttgttgttgt ttgaggcgtt gttttaagtg
     2281 ttcgtttttt tctaagaaaa attgtcgtgc ttcatcggca gcttcttgcg cctcgcggtg
     2341 ttcttggtta agtttttgaa aatagttttt ctctttttgc aattcgccta gcaaatgatc
     2401 cattttcacc cgtttatcgt ctaatcgctt ttttgcttcg tcgataatgc gttgcgaaat
     2461 accattgttt tgcgccactt caaaggtgaa agaacttcct ggttgcccta cgctaaattg
     2521 ataaagtggc tccaaggtat ctgtatcaaa cagcatgcac ccgttgacag cttgcggcaa
     2581 ggcatcggct tttagtttaa tgttggcgta atgcgttgtg ataaccgcaa aacactcttt
     2641 tttatacaat tcttcaaaaa atacttcggc caatgcgcct cccaattcag ggtcggaacc
     2701 tgttccaaat tcatcgagca acaacaacgt gcggcggttg gttactttta aaaagtactt
     2761 cattcttcgc aaacggtaag aataggtact caattcattt tcaatagatt gattatcccc
     2821 aatatccgac aacacttgct gaaagaagca gattttactg ttgggatgaa cgggaattaa
     2881 aattcctgcc tgcaacatca cttgcaacaa tccgatggtt ttgagtgtaa tactttttcc
     2941 gccagcattg ggtccagaaa taaccaacat acgggctttg ttgtgcaatt gaatgtgttg
     3001 aggaaaggtt tttttgccca ccaatttatt ggatttccac aaaattggat gaaacgcctc
     3061 catcaaatca atatgcatgg aacggttgat gcccggcaaa tgacaattta attcgagtgc
     3121 caaacgtgtt tttgcgttga tgaaatcaaa ttctgtcaat acttgttgat aggcttcaat
     3181 gagcggaagg taaacagcaa tttcggctgt gagcacttta agaatgcggt aaatttcttt
     3241 gcgttcttca tcaatttgca attccaattc attgttgagt tccacattga tttgcggctc
     3301 cacatacacc aaactccctg ttttactgga gcctacaatg tttccagcaa ccgaacgttt
     3361 gtgcgaagca agcacggtca acaccctgcg ttcattataa aaagcttcgt gggtatcgcc
     3421 caatatgttg tcttttgcca agcgacgcat ttctttatcg aaatttttat tgatttgttg
     3481 gcgcagcaat ttaatttccg agcgtatttc ggccaataat ggcgaagcgg catccaccac
     3541 cttccaactt ttatcgaaaa tggcatcaat tttttcaata atatctttgg tatattccac
     3601 ctgctctact gttgcagcca aaagtggata atctttttcg cgtttattaa aaaaataaac
     3661 caactgattg gcaacgttgg aagcaagacg aatagtgtaa aatccctcat gcgaaagaac
     3721 cgcatttttt attgccagca gtttaatttc ttgtgttaat tcttcaaaat ccaacgatgg
     3781 aaattgttca cccgttttgc gaatggcaag taactcatta gatttaagca actctgtttc
     3841 aaccgcttta aaatcattac tcggctctag ttgattgatt ctagcttttg cagtagggcc
     3901 gacagcatat ttctccaacc attgctgaat agttggaaac tccaaatctt ttagtgtttg
     3961 cttatcgaac gaagaattca ttgcgcaaat ttaagcactt taagtgttaa aaaataaatt
     4021 cagataaaaa tgtatgaaac agcaaataat tcaatgaaat ttgtatgttt gtataactta
     4081 ataaacaatt atgagaaaac taattatcgc ttgttcactt ttattgttgg gaactacagc
     4141 aaatgcacaa aaaggatttt atacagacat taactttgga tatggcgtgt gcattccaaa
     4201 ttctgttata gcagttgatt tgtataaaaa cttagacaat ccagatttat cctatcaacg
     4261 caatgtttac ggtacgcttg gtagaggttt taatgtttct gttaaccctg gctatatgat
     4321 taccaagcac attggtgtgg aattgggcat caattatttt attggagcag aatccatgtt
     4381 ggataccaat aaaaccactt ctgaccctaa tttatatgac gttaccaagg caaaatccaa
     4441 tcaattgcgc atcattccgg cagtagtaat taacacagga ggaagtaaat tgtatggata
     4501 tgccaaagct ggtttagttt taccaatagc tggaagcaca gaagctgttc gttcggcatc
     4561 ttttagtgtt ttggtggatc caataaacca cgttaaacaa gtttattcta acgagtttaa
     4621 ggcaactgtg aacggaagtt tttcattagg atttaaaggt tcattgggaa ttggttataa
     4681 tttcacacct aatattgggt tgaatttaga agttttttgc acggcattgc aggtaaaagc
     4741 gaaaacaaga aaagtggttg aaacagctaa agttgacggt gtggttgtgg aaaatgatcc
     4801 ggcaccaact tattatacag aaaccaatta tgtggataat ttatcgtcta actccaacaa
     4861 cgataaatac aatccaaatt ttgatgtaac aaaacccaaa gatgaattgg cgcgcaaaac
     4921 aagctttaat caatttggat ttaattttgg tattactttt aaagttggat ctaaatctac
     4981 taaagagtaa taaaatcatc tattttattg gaaaagtgga gagattttct ttccactttt
     5041 ttgttgcttt aaaattattt catccttttt tgtatagaga agtaaaaaaa agaataccaa
     5101 ggcacaaaaa aaggctactt aaaaaagtag ccttttacta ttttattaga gtaatattac
     5161 attcccatgt ttttcaaatc atccattgat gcttcttgga aaccttcagg aatatccata
     5221 ttaaacaatt tagctttatc ttctttaatt ccttttgcca cttctgttgc agtgaatttc
     5281 ataactacac cattgttagc caattcaaat tccaatgggt aaccttcaac tttttcgttc
     5341 atgaatttat ttccggcttt agaacccgac aattctgttg ttgtccaata attcattgtt
     5401 gtgccatctt ccgtagttaa aatgtattgt gtacatttat aacctgcaat tttcttagtt
     5461 tttgacgttt tttcaacttc tactttagct tcttcaccat cttctttgtc tagttcgtcc
     5521 aaatttgttt taattgcttt tttagaaccc attccgtcca ttaacatcaa cgattgttta
     5581 gatttcaaat caatgatagt tgacgttttc ataaacattc ccatgtttga ttcaacacgt
     5641 gtaaattcag gtgtaaaata agtggtcatt gtagaaccgt tcaacattgc caattgtgat
     5701 tggatttgag gatctgagct tttaaattct gtggtgtact taacaactcc ttctgttgtt
     5761 tgtgcaaatg aaccaaccgc aacggtcaat cctaaaaata aagtcaatag ttttttcatt
     5821 ttattcgttt taaatttgtt ttcgaaatta acacaattat tgcaccaaag tttttgagga
     5881 cttaattttt atcatatttc tacatatttt tggatttttt taaccaaatt agcgttgaat
     5941 caaaggtcga attagggatt ctaagccatt gagtttaatt tcgtaaatta gctgtaattg
     6001 ctctccaatt ttacctttcg ggaaaccttt attgcgatac cacactatgt aatactccgg
     6061 tagatcaatt aaataatagc cttggtattt gccaaaaggc attttggtat ttgctaattg
     6121 aattaactgt tcatttgctg atatttctcc cattggtggt tccacttttc tacaaattta
     6181 taaaaacgaa gaattttacc taaaaattag caactcaaat attgctgcaa acattaaaaa
     6241 aggctgaaaa atagggtatt ataatcgatt tggatgttta aggataatta gtatatttgg
     6301 tgagattaga ggtaaagagt tggcaaccta tttattctaa acttaaaacc gatatagaaa
     6361 actaaaataa acactacaat gaaaagagaa ttagcaaagg acattataca atgggacata
     6421 aaatcttggt ctaaagcact taggtattgg gaaagccaaa ttgattgggg taaaataaaa
     6481 aatgggcttg aactaggagg acgagaaggt ggactttcac tttggcttgc tttaaaagag
     6541 aaagatgtgg tttgttctga cttgaaagac gttcaaaagt cagctgaaaa actacacatc
     6601 cgacacaacg ttacttcgcg tattatatac caagacattg acgcaacaaa tattccatac
     6661 gaaaattatt ttgacatcat tgtatttaaa tctattatag gcggaattgg aagaaatgac
     6721 aaacatgaaa atcaacaaca ggttttcaaa gaaatataca aagcacttaa gcctggtgga
     6781 aaacttttgt ttgctgagaa tctaattg
//
LOCUS       DAOQVQ010000078         6800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12327_length_6800_cov_3.692809, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000078 DAOQVQ010000000
VERSION     DAOQVQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12327_length_6800_cov_3.692809"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN22_03705"
     CDS             <1..516
                     /locus_tag="PKN22_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS41842.1"
                     /translation="HGQKGFQKLFMSYAMQVITSCDIPFVIVQKNTPIKNLNKIVIPI
                     DLTKESLQVVNIAGDLATMFDAEVHVIGEKQTDEHLNITLKNRIYIINNRYEERNIKC
                     HIEQVKSGGSYSAKVLEYAEKNSIDMIAIAYHTESILPQFDKFAQNLITNDKMLPCLV
                     LNSKLASSLYY"
     gene            518..1348
                     /locus_tag="PKN22_03710"
     CDS             518..1348
                     /locus_tag="PKN22_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNS41843.1"
                     /translation="MKVGEINTLRILRFTSVGAYLGDEKDNDILLPNKYLTDDMLLDD
                     DIDVFVYRDSEDRLVATTEKPFVNLNEFALLSITDVNFFGAFADWGLEKELMIPFKEQ
                     HLKLAIDKSYIVTLQRDDATDRLFGSTKVNRYLIKCEDESVINKAQKILITESTDLGF
                     KCIVSNKYNGLIYHSDFNNAFKRGDIVEGYIYNIRKDGKVDIRLGKPGYLRIDENVEK
                     LSKILQRLGKLSITDKSSPELIQETVGMSKKVFKQAVGNLYKQRLIALKEDGIVWLNN
                     "
     gene            complement(1345..1710)
                     /locus_tag="PKN22_03715"
     CDS             complement(1345..1710)
                     /locus_tag="PKN22_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNS41844.1"
                     /translation="MESKKTLVIGASTNPERYSFKAISMLRAHQHEVEAIGLKSGEVA
                     GVLIHTEKHPFEDINTVTLYVNPARQVDFYDYIIGLRPKRVIFNPGTENSEFETMLSN
                     NNIAYEEACTLVLLSTGQY"
     gene            complement(1720..2595)
                     /locus_tag="PKN22_03720"
     CDS             complement(1720..2595)
                     /locus_tag="PKN22_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41845.1"
                     /translation="MEKKWDGKTKGSLWGYRFFIALIRFLGVRISYFFCFWVTGYFVL
                     FASKQRKALVQFYQRGFGFNKWKSVRFATRTFYEFGTIIIDRIALRTERKKIYTHSFE
                     KEEVLRNIHQAGKGGFLISGHVGNWENAGSLIGERITSKINVLMLDAEVEKIKQYIDE
                     SVEQVNYNLIPLKDDLSHLILIHKALKNNELIALHADRITEGQKTFTISFLNQPTQFP
                     AGPFIMAFKFKVPVTFVFAVKDKFHFSLSATDALIGGVDVQSPEEMAQLYVHRLEELV
                     KKYPNQWFNFYNYFD"
     gene            complement(2602..2850)
                     /locus_tag="PKN22_03725"
     CDS             complement(2602..2850)
                     /locus_tag="PKN22_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNS41846.1"
                     /translation="MNREEIIATTKRFLSEEFEIDQALIQPNNNLHKTLDLDSLDYVD
                     LVVVIDENLGFKPTSDDFKSIQTFEDFYQLVEEKLALA"
     gene            complement(2879..4102)
                     /locus_tag="PKN22_03730"
     CDS             complement(2879..4102)
                     /locus_tag="PKN22_03730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNS41847.1"
                     /translation="MEQRVVITGIGIYSCLGENLNEVLTSLKQGKSGIIYDETRKDFG
                     YRSCLTGHVNIPDLKPFLSRRERIGMHEPAMFAYMSTMEAMKNAQLDVDFLEKTETGI
                     IFGNDSTAGAVIETIDKVRERKDTTLIGSGDIFQNMNCTVNMNLSTIYKLKGINLSLS
                     AACASGSHSIGLGYLLIKQGLQDRVVCGGAQETNLYAMASFDGLGAFSIRQDTPTKAC
                     RPFDRDRDGLVPSGGAATVIIESLESALKRNAPIYGEIIGYGFSSNGEHISNPNIDGQ
                     HRSLNMALKNAGISANEIDYVNAHATSTPIGDATEAEAIFDVLGGKVPVSSTKSMTGH
                     ECWMAGASEIVYSLLMMENNFIAPNINFENPDEHSAKINVVPTYKEHQIDCFLSNSFG
                     FGGTNSSLIVKKFKK"
     gene            complement(4104..4835)
                     /gene="fabG"
                     /locus_tag="PKN22_03735"
     CDS             complement(4104..4835)
                     /gene="fabG"
                     /locus_tag="PKN22_03735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNS41848.1"
                     /translation="MKCALVTGASRGIGRAVAIQLAKDLNLHILINFAGNEAAAKETL
                     QTIESNGGTAELMQFDVKNNAEVTEKITAWIEANPDKNIEVLVNNAGITRDNLFPWVN
                     EKDWDDVINTSVKGMYNCTQAVIGKMLRNRSGRIINVASLSGLKGNPGQTNYSAAKGA
                     MIASTKALAQEVAKRKITVNAVAPGFIASDMTNNLDEAQLKQLVPANRFGQPEEVAEL
                     VCFLASDKAAYITGEVININGGLYS"
     gene            complement(4832..6361)
                     /locus_tag="PKN22_03740"
     CDS             complement(4832..6361)
                     /locus_tag="PKN22_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNS41849.1"
                     /translation="MHQLTLVDIENIAINKQAFVIDSKTIEKVKKSFAFLEDFSSDKI
                     IYGINTGFGPMAQFKIDEDKLNQLQYNLIRSHSSGTGHALSPEEARAVLIARANNFLQ
                     GNSGISYEVIEKINEFLAHDIAPEIFEHGGVGASGDLVQLAHLALNLIGEGYVYYKGE
                     RQKTAAVFSELNIQPLNVKLRDGLGLMNGTSCMTGIGALNIIYAKRLLDWSVATSAII
                     NEIVESFDDSFSEGLNQVKQHKGQEIVAAKMRSMLADSKLIRSREELFADDTALQQTT
                     FKKKIQEYYSIRCIPQILGPVADTLDFAQNILEQELNSTNDNPVVREDLGNVFHGGNF
                     HGDYVSLEMDKVKIVATRLTMLAERQLNFLLNSKINDIFTPFLNAQTLGFNFGVQGMQ
                     FTATSTTAECQTLSNPMYVHSISNNNDNQDIVSMGTNSAVIARKVIENGFQVMAIHAM
                     AICQAIDLLPTEKQEKLSSKGKEIYRLIRSKAPMINEDVAQFENIKAVTQLLRNTKLE
                     L"
     gene            complement(6380..>6800)
                     /locus_tag="PKN22_03745"
     CDS             complement(6380..>6800)
                     /locus_tag="PKN22_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNS41850.1"
                     /translation="NNINQNFADIKDRVHYYPINVLDEQSTIPAGADVYWMSQFLDCF
                     SEAQIESILLKIKENAKPGAKVYIMETFIDNQRFPAAEFSLVATSLYFTVMANGNSKM
                     YSSSVFKYIIDKAGFKCVGEHHLHEDSYHTILELELK"
BASE COUNT         2102 a         1261 c         1198 g         2239 t
ORIGIN      
        1 cacggacaaa aagggtttca aaaattattt atgagttatg ctatgcaagt aatcactagt
       61 tgtgatatac cgtttgtaat tgtacaaaaa aatacaccaa ttaaaaattt gaataaaatt
      121 gttattccta ttgatttgac caaagaaagc ttgcaagttg ttaatattgc aggagattta
      181 gcaaccatgt ttgatgctga agttcatgtg attggtgaaa aacaaaccga tgaacatttg
      241 aatattacat taaaaaatag aatttatata attaataatc gttacgagga acgaaatatt
      301 aaatgccata tcgaacaagt taaaagtggg ggttcttatt ctgcaaaagt gcttgaatat
      361 gctgaaaaga atagcataga tatgattgca attgcctatc atactgaaag tattttacca
      421 caatttgata aatttgctca aaacttaatt acaaacgata aaatgttacc ttgcttggtg
      481 ttaaattcta aattagcatc ttctttatat tattaaaatg aaagtcggag aaataaacac
      541 cttaagaatt ttacgtttta caagtgtagg agcctattta ggggatgaaa aggataacga
      601 tattcttctt cccaataaat acttaactga tgacatgcta cttgacgatg acatcgatgt
      661 ttttgtttat cgcgattctg aagatagatt ggttgcaaca actgaaaaac cttttgtaaa
      721 tctaaacgaa tttgccttgt tatcaattac cgatgtgaat ttctttggtg catttgccga
      781 ttggggcttg gaaaaagaat tgatgattcc gttcaaagaa cagcacttga aattagcgat
      841 agataaatcg tatattgtaa ctcttcaacg tgatgatgct accgatcgat tgtttggttc
      901 aacaaaagtt aatcgctatt taattaaatg cgaagacgag tcagtaatta ataaagctca
      961 aaaaatattg attactgaat caacagattt aggctttaaa tgtatcgtct ctaacaaata
     1021 taatggactc atttatcatt ctgattttaa taatgcattt aaaagaggtg atattgtaga
     1081 aggctatatt tacaacattc gtaaggatgg aaaagtggat atccgtcttg gaaaaccagg
     1141 gtatttacga atcgatgaaa atgtagaaaa gttaagtaaa attttacaac gattaggtaa
     1201 attaagtata acggataaaa gctctccaga actcattcaa gaaactgtag ggatgagtaa
     1261 aaaagtattt aagcaagcag ttggaaatct ctacaagcaa cgtttaattg cgttaaagga
     1321 agacggaatt gtttggttga acaattaata ttgtccggta ctcaataaaa caagcgtaca
     1381 agcttcttcg taggcaatat tgttgttcga aagcattgtt tcaaactcgc tattttctgt
     1441 tccaggatta aagataacgc gtttcggacg caatccaatg atgtagtcgt aaaaatcaac
     1501 ctgacgggct ggatttacat acaaggtcac tgtgtttatg tcttcaaatg ggtgtttttc
     1561 ggtgtgtatt aaaacacctg caacttctcc agattttagt ccaattgctt ccacctcatg
     1621 ctggtgtgca cgtaacatcg aaatggcttt aaacgaataa cgctcaggat ttgtgctagc
     1681 accaatgacc aaagtttttt tagattccat atccgaaagt taatcaaaat agttgtaaaa
     1741 attaaaccat tgattgggat attttttcac taattcttct aatcgatgaa cataaagttg
     1801 cgccatttcc tcgggagatt gtacatctac acctccaatt aatgcatctg tggcacttaa
     1861 cgaaaaatga aatttatctt ttacggcaaa tacaaaagta actggaactt tgaatttaaa
     1921 agccatgatg aacggtccag caggaaattg cgtcggttga ttcaaaaagg aaatggtaaa
     1981 tgttttttga ccttcggtaa tacgatcggc atgtaaggca attagttcat tattttttaa
     2041 agctttgtga atgagaatca aatgcgataa atcgtctttt aacggaatga gattgtagtt
     2101 gacttgttcc acactttcat caatgtattg ctttattttt tcaacttctg catccaacat
     2161 taaaacgttt attttagatg tgatgcgttc gccaattaaa cttcctgcat tttcccaatt
     2221 accaacgtga ccagaaatta aaaaaccacc tttacctgct tggtgtatgt ttctgagtac
     2281 ttcttctttt tcaaaagaat gggtgtaaat cttttttcgc tctgtgcgca aggcaattct
     2341 gtcaataata atggtgccaa attcataaaa agtacgcgtt gcaaaacgta cacttttcca
     2401 tttattaaag ccaaatccac gttggtaaaa ttgcactaat gctttgcgtt gtttagaggc
     2461 aaatagtaca aagtagccag ttacccaaaa gcagaaaaaa taagaaattc gtacgcctaa
     2521 aaagcgtatc aatgcaatga aaaaacggta accccaaaga gaacccttag ttttaccatc
     2581 ccattttttt tccatgaaaa attatgctaa agccaatttc tcctcaacca attggtagaa
     2641 atcttcaaat gtttggatag atttaaaatc atcagacgtt ggtttgaatc ccaaattttc
     2701 gtcaataaca acaactaaat ctacatagtc caaactatcc aaatccaacg ttttatgcaa
     2761 attgttgttt ggctgaatca acgcttgatc aatttcaaat tcttcagata aaaagcgttt
     2821 tgtggttgca ataatttctt ctctattcat gagttataaa tgttagtgca aatgtagctt
     2881 attttttaaa ttttttaaca attaatgatg aatttgttcc tccaaatcca aaagaattgg
     2941 ataaaaaaca atcaatttga tgttctttgt aggttggtac aacattgatt ttagccgaat
     3001 gttcgtctgg attttcaaaa ttaatattgg gggcaataaa gttattttcc atcattaaaa
     3061 gtgaatacac aatttcactt gcacctgcca tccaacattc gtgaccagtc atacttttag
     3121 tggaactcac tgggactttc ccaccgagca catcaaaaat agcctcagcc tcggttgcat
     3181 ctccaattgg cgttgaagtg gcgtgcgcat tcacataatc aatttcattg gcagaaattc
     3241 cagcattttt caaggccatg ttcaagctgc ggtgttgccc gtcaatgtta gggttagaaa
     3301 tgtgttcacc gttggatgaa aatccatagc caataatttc accataaatg ggagcattgc
     3361 gctttaatgc cgattctaaa ctttcaataa tcaccgtggc agcaccacca gaaggaacca
     3421 atccatctct atctctatca aacgggcgac atgctttggt tggtgtgtct tggcgaatgg
     3481 aaaatgctcc caatccgtca aaacttgcca ttgcatataa attggtttct tgcgctccac
     3541 cacataccac acggtcttgc aatccttgtt tgattaacaa atatcctaaa ccaatggaat
     3601 gagaacccga cgcacaagca gccgaaagtg ataaattgat gccttttaat ttgtaaatgg
     3661 tcgataaatt catgttgacc gtgcagttca tattttgaaa aatatcgcca ctaccaatca
     3721 atgtggtgtc tttgcgctcg cgaactttat caatggtttc aataactgca ccagccgtag
     3781 aatcattgcc aaaaataatt cctgtttcag ttttttctaa aaaatcaacg tccaattgcg
     3841 catttttcat ggcttccatc gttgacatat aagcaaacat tgcaggttcg tgcataccta
     3901 tgcgttctct acgcgataaa aaaggtttta aatcaggaat gttgacatga ccagttaagc
     3961 acgaacggta accaaaatcc tttctcgttt catcgtaaat gatgccgctt tttccttgtt
     4021 ttagggaggt taaaacttcg tttaaattct ctcctaagca agaataaata ccaattcctg
     4081 tgatgactac tcgctgttcc atgctatgaa tacaatcctc cgttaatatt aataacctcg
     4141 cctgtgatat aagccgcttt gtcggatgct aagaaacaca ccaattctgc cacttcttcg
     4201 ggttgcccaa aacggttggc tggaaccaat tgttttaatt gtgcttcgtc caaattattg
     4261 gtcatatcag aagcaataaa tcctggagca acggcattta ccgtaatttt gcgttttgct
     4321 acttcttgag ctaatgcttt ggtagatgca atcatggcgc cttttgctgc cgaatagttg
     4381 gtttgacctg ggtttccttt caatccactc aatgaagcaa cgttgatgat gcgccccgaa
     4441 cggttacgca acatttttcc aataactgct tgcgtgcaat tgtacatgcc tttcacgctt
     4501 gtattgatga catcgtccca atctttttca ttcacccaag gaaataagtt atcgcgtgta
     4561 attccagcat tgttgaccaa cacttcaata tttttatccg gattagcttc tatccaagca
     4621 gtaatttttt cggttacttc agcattgttt tttacatcaa attgcattaa ttcggctgtt
     4681 ccaccattgg actcaatggt ttgcaatgtt tcttttgcgg cagcttcgtt tcctgcaaaa
     4741 ttgattagaa tatgcaggtt caaatctttt gctaattgta tggcaactgc tcttccgatt
     4801 cctctagaag cacctgttac taatgcacat ttcatagttc taattttgta tttcttaata
     4861 attgtgtgac tgctttgatg ttttcaaatt gcgccacatc ttcattaatc attggagctt
     4921 tggagcgaat taaacggtaa atttctttcc ctttcgagct caatttttct tgtttttcgg
     4981 ttggcaataa atcaattgct tgacatatag ccatagcatg aatagccatt acttgaaatc
     5041 cattttcgat gacttttcgg gcaattacag cactgttagt tcccatactc acaatgtctt
     5101 gattgtcatt gttgttggaa atactgtgca catacatcgg gttggaaagc gtttgacatt
     5161 cggccgttgt ggatgttgcc gtgaattgca ttccttgaac tccaaaatta aatcctaatg
     5221 tttgcgcatt taaaaatgga gtgaaaatat cgttgatttt actattgagc aagaaattta
     5281 attggcgttc tgcaagcatc gttaggcgtg tagctacaat ttttactttg tccatttcca
     5341 acgaaacata atcgccgtgg aagttgcctc catgaaaaac attgcccaaa tcttcacgaa
     5401 caactgggtt gtcgttggta gaattcaact cttgttctag tatgttttgt gcaaaatcca
     5461 aggtgtcggc aactggaccc aaaatttgag gaatacaacg aatggaatag tattcttgaa
     5521 ttttcttttt aaacgttgtt tgttgcaagg ctgtatcgtc ggcaaaaagt tcttcgcgac
     5581 ttctaatcag cttcgaatcg gccaacatag aacgcatttt agcagccact atttcttgac
     5641 ctttgtgttg ttttacttgg ttcaatcctt ccgaaaaaga atcatcaaac gattcaacta
     5701 tctcattgat gatggcagat gttgcaaccg accaatccaa caatcgtttg gcataaatga
     5761 tgttcaaagc accaattccc gtcatgcacg aagttccgtt cattaagccc aaaccatcgc
     5821 gtaattttac gttaagtggt tgaatgttta attcactaaa aacggcagca gttttttggc
     5881 gttcaccttt gtaatacaca tagccttcac caattaaatt caatgccaaa tgtgccaatt
     5941 gaaccaaatc accacttgca ccaacgcctc cgtgttcaaa aatttcggga gcaatatcgt
     6001 gcgctaaaaa ctcatttatt ttttctatta cttcgtaact aatcccagag ttaccttgca
     6061 aaaagttgtt ggcacgtgca attaatacag ctctagcttc ttctgggcta agtgcgtgac
     6121 cagtaccaga ggaatggcta cgaatcaagt tgtattgtaa ttggtttaac ttgtcttcgt
     6181 caattttaaa ttgcgccatc ggaccaaaac ccgtgttgat gccgtaaata attttatcgc
     6241 ttgaaaaatc ttctaaaaaa gcaaacgatt ttttgacctt ttctattgtt ttggagtcaa
     6301 ttacaaatgc ttgtttattg attgcaatgt tttctatatc aactaatgtt aattgatgca
     6361 taagattatt gagttgttgt tattttaatt ctaattctaa aatagtgtgg taactatctt
     6421 cgtgtaaatg gtgttcgcct acgcatttga acccagcttt atcaatgatg tatttgaaaa
     6481 cgctggaaga atacatttta ctgtttccgt tggccataac ggtaaaatac aacgatgtgg
     6541 caactaatga aaattctgca gctggaaaac gttggttatc aataaacgtt tccatgatgt
     6601 acacttttgc accaggtttt gcattttctt tgattttcag taagatggat tctatttgcg
     6661 cttcactaaa acaatctaaa aattgactca tccagtacac gtcagcacct gccggaatgg
     6721 tggattgctc atccaaaaca ttgatggggt aataatgcac acggtcttta atatcagcaa
     6781 aattttgatt gatgttgttt
//
LOCUS       DAOQVQ010000079         6758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12433_length_6758_cov_4.594957, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000079 DAOQVQ010000000
VERSION     DAOQVQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12433_length_6758_cov_4.594957"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /gene="rpsA"
                     /locus_tag="PKN22_03750"
     CDS             <1..1341
                     /gene="rpsA"
                     /locus_tag="PKN22_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNS41851.1"
                     /translation="GIEAFLPGSQIDVKPIRDYDVYVGKNMEFKVVKINQEFRNVVVS
                     HKALIEAELEEQKKQIISGLEKGQVLEGIVKNVTSYGVFIDLGGVDGLIHITDLSWGR
                     VNHPEEIVELDQKLNVVILDFDDDKRRIALGLKQLQPHPWDALDANIAVGDKVSGKVV
                     VIADYGAFIEIAPGVEGLIHVSEMSWSQHLRSAQDFLAVGDTVEAVVLTLDREERKMS
                     LGIKQLFPDPWNGIEDKYTVGSKHTAKVRNFTNFGVFVELEEGVDGLIHISDLSWQKK
                     IKHPSEFCKVGDEMEVVVLEIDRENRRISLGHKQLEENPWDVFETIFGEGSVHQGTII
                     DTKDKAGIVALPYGVEGICPVKHLKKEDGSNAKVDEVLDFKVIEFNKEAKKIVLSHLR
                     TYKEGDDATPATKGGKKGDSTSSTVNAINQSNEKSTLGDLDALAELKEKMEKEK"
     gene            1462..2466
                     /locus_tag="PKN22_03755"
     CDS             1462..2466
                     /locus_tag="PKN22_03755"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNS41852.1"
                     /translation="MSKITARFPSNIALVKYWGKYGYQLPCNPSISLTLKHATTEVVL
                     EKKEKTKAGIELDYLFEGKTNEAFLARMVSYIEKQPEFTSLFENFAVQIHSKNTFPHS
                     TGIASSASAYAAIAGALLTASNYTASDFKQVASNLARKGSGSACRSYFGPFALWGELQ
                     EVENSSNEYAVPLNTVHSNFQTLKDAILIVEDTPKKVSSSVGHGLMKNHPYAEARFID
                     AKKHATEMLSTLKGGDFNQFISITEREALSLHAMMMTSGDYYLLMKPGTIQIIEQLFD
                     FRNATGLPICFTLDAGPNVHLLYPPSIEEQAKEFIASVQHHTKSVIFDEAGFGGEIIG
                     "
     gene            2467..3423
                     /locus_tag="PKN22_03760"
     CDS             2467..3423
                     /locus_tag="PKN22_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNS41853.1"
                     /translation="MQQQVEQFNSKILLFGEYAVLHNGMALVIPCEKFHGHFEFYQGG
                     SNEEQAVALQSNEYLRKFCAFVSSRMDEKFVLEVKKFEAELEQGLFFRSNIPQGYGLG
                     SSGALVAAMVLRYLVKAKNIKDEVKLQTYLKLKDLKKNLGNLESFFHGTSSGIDPLSS
                     ILNEPILCKNANEIVTTTLPLTAPDKQNVIFLLDTKQERTTTKMMALFNELLGKEEFK
                     NKFDKYVIENNNGAIQSFLDNDNKRFYEVVNELSAFQINYMKDFFPESLHKDVQKGLD
                     NGDYFLKLCGAGGGGFVLGFTQNWEQTKEQLNNYELEEIYRF"
     gene            complement(3483..5480)
                     /locus_tag="PKN22_03765"
     CDS             complement(3483..5480)
                     /locus_tag="PKN22_03765"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNS41854.1"
                     /translation="MTQQEFFNDILQGVPAQIPAKKAYEPNINHAPKRKAILSEDEKK
                     LAVRNALRYFPKEQHAELAKDFADELNQYGRIYMYRYRPDYKLFARSIDEYPGKSLQA
                     KAIMLMIQNNLDYAVAQHPHELITYGGNGAVFQNWIQYRLTMKYLAEMTDEQTLVMYS
                     GHPMGLFPSHKDAPRVVVTNGMMIPNYSQPDDWERFNALGVTQYGQMTAGSYMYIGPQ
                     GIVHGTTITVLNGFRKIGKTIKGGLFVTAGLGGMSGAQPKAGNIAGCISVTAEVNRNA
                     AEKRHSQGWVDELFDDVHLLAERVRKAQSNKEVVSLAYVGNVVDVWEYFLAQNIQIDL
                     GSDQTSLHNPWAGGYYPVGLTYEEANEMMATNPELFKEKVQASLRRQVEAINGHTAKG
                     TYFFDYGNAFLKEASRAGADIMAPNGIDFKYPSYVQDIMGPMCFDYGFGPFRWVCASG
                     KPEDLHITDELAKEVLLRLIQDSPAEIQQQMKDNIQWIEGAQANKLVVGSQARILYAD
                     ALGRMEIAKAFNDAIAAGKIGPVVLGRDHHDVSGTDSPFRETSNIYDGSRFTADMAIH
                     NVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMLLDGSSDSERRLKSMLHWDVNNGIS
                     RRSWARNEGAVFAIKRAMEMEPNLKVTVPEMVDDTIINGLF"
     gene            5685..>6758
                     /locus_tag="PKN22_03770"
     CDS             5685..>6758
                     /locus_tag="PKN22_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNS41855.1"
                     /translation="MNDQLKDALKQLTDIIDSETEFIPLITSDEEETMNNTSYPDILP
                     ILPLRNNVLFPGVVIPITVGRDKSIKLIQAANKGDKIVGVVSQKDQEDESPEFDDLHK
                     IGTVAQIIRLLKMPDGSSTAIIQGKRRFELVEPIQSEPFIKARVQYLEEPKVDYTSKE
                     MTFLIDSVKESALQIIKESPNIPSEASFALKNIDSPSFLINFVSSNMNADVAKKQSIL
                     DELDAKKRLMLVLEQLNLEVQMLQMRNEIQSKVKKDIDRQQREYFLHQQIRTIQDELG
                     GSPHEMDVQELQERALRKKWDEKTEKVFYKELEKLKRTNPQGAEYTVLLNYLDLLLDL
                     PWGVYTKDIHDLKRASKVLERDHY"
BASE COUNT         2171 a         1264 c         1315 g         2008 t
ORIGIN      
        1 ggtatcgaag cattcttacc aggttctcaa atcgatgtta aacctatccg tgattacgat
       61 gtttacgtag gtaaaaacat ggagttcaaa gtggttaaaa tcaaccaaga attccgtaac
      121 gttgttgttt ctcacaaagc acttattgag gctgagttgg aagaacaaaa gaaacaaatt
      181 atttctggtc ttgaaaaagg tcaagtattg gaaggtatcg ttaaaaacgt tacatcttac
      241 ggtgtattca tcgacttggg tggtgttgac ggtcttatcc acattacaga tctttcttgg
      301 ggacgtgtta accacccaga agaaatcgtt gagttggatc aaaaattaaa cgttgtaatc
      361 ttagacttcg atgatgataa gagacgtatt gcattaggat tgaaacaatt gcaaccacat
      421 ccttgggatg ctttagatgc taacatcgct gttggtgata aagtttctgg aaaagttgtt
      481 gttattgctg actatggtgc attcatcgaa atcgctccag gagttgaagg attaatccac
      541 gtttctgaaa tgagctggtc tcaacattta cgttctgctc aagatttctt ggcagttggt
      601 gatactgttg aagctgttgt tttaacatta gaccgcgaag agcgtaaaat gtcattaggt
      661 atcaaacaat tgttcccaga tccatggaat ggaattgaag acaaatacac agtaggttct
      721 aaacatacgg ctaaagttcg taacttcact aactttggtg tattcgtaga attagaagaa
      781 ggtgttgacg gattgattca catttctgac ttgtcttggc aaaagaaaat caaacaccca
      841 tctgaattct gcaaagtagg agacgaaatg gaagtggttg tattggaaat tgaccgcgaa
      901 aacagaagaa tctctttggg tcacaaacaa ttggaagaaa atccatggga tgtatttgaa
      961 actattttcg gagaaggttc tgtacaccaa ggaacaatta tcgacacaaa agataaagca
     1021 ggaatcgtgg ctcttccata cggtgttgaa ggaatttgtc cggttaaaca cttgaaaaaa
     1081 gaagatggtt ctaacgcaaa agtggacgaa gtattagatt tcaaagtgat tgaattcaat
     1141 aaagaagcta agaaaatcgt tctttctcac ttgcgtactt acaaagaagg tgatgacgct
     1201 actccagcaa caaaaggagg taaaaaaggt gacagcactt cttcaactgt aaatgctatc
     1261 aaccaaagca atgaaaaatc aactttaggt gatttggacg cattggctga attaaaagaa
     1321 aaaatggaga aagagaaata agctcttcac cccataaaat tggaaagccg gttctttttt
     1381 gaatcggctt ttttattttc aacatccgca aaacggattt gatttctaaa atcagctatc
     1441 tttgcaatca aaattcgtgg tatgtcaaaa attacagctc gttttccttc caacattgcc
     1501 ttggtaaaat actggggaaa atacgggtat caattgcctt gcaatccttc aattagttta
     1561 acgttgaaac acgctacaac agaagttgtg cttgaaaaaa aggaaaaaac aaaagctgga
     1621 attgaactcg attatttgtt tgaaggaaaa accaacgaag cttttctggc tcgtatggtg
     1681 agctacattg aaaaacaacc tgaatttact tcgctttttg agaattttgc cgttcaaata
     1741 cattcaaaaa atacctttcc tcactctaca ggtattgctt catcggcttc ggcttatgca
     1801 gctattgctg gggctttgtt aactgctagt aattatactg caagcgattt taaacaagta
     1861 gcaagcaatt tggcacgcaa aggaagtgga agcgcttgtc gttcttattt tggacccttt
     1921 gctttgtggg gcgagttgca agaagtggaa aattcaagta atgaatatgc agtgccacta
     1981 aataccgttc attccaactt tcaaacattg aaggatgcta ttttgattgt tgaggatacg
     2041 cctaaaaagg tttcaagcag cgtgggccat ggtttgatga aaaaccatcc ttatgccgaa
     2101 gcacgtttta tagatgctaa aaaacatgct accgaaatgt tatcaacttt gaaaggtgga
     2161 gattttaatc aatttatttc catcaccgaa cgagaagcat tatctttgca tgcaatgatg
     2221 atgacttcag gtgattatta cttgttgatg aagccaggaa cgattcaaat aattgaacaa
     2281 ttgtttgatt ttagaaacgc aaccggcttg ccaatttgtt ttacactcga tgcagggcct
     2341 aatgttcatt tgctttaccc accttcaatt gaggagcaag caaaagaatt tatcgcttct
     2401 gtgcaacatc atacaaagtc agttattttt gatgaagctg gttttggtgg agaaattata
     2461 ggataaatgc aacaacaagt agaacaattc aattccaaaa ttttgctgtt cggtgaatat
     2521 gccgttttgc acaatggtat ggcgcttgtc attccgtgtg aaaaatttca tggccacttt
     2581 gagttttacc aaggtggaag caatgaggaa caagctgttg ctctacaatc caatgaatac
     2641 cttcgtaaat tttgtgcttt tgtttctagc cgcatggatg aaaaatttgt gttggaagtg
     2701 aaaaaatttg aagccgaatt agagcaagga cttttcttta gaagcaatat tccacaagga
     2761 tatggtttgg gaagctcggg cgccttggtt gctgcaatgg tattgcgtta cttggtaaaa
     2821 gctaaaaaca tcaaagatga ggtgaaattg caaacctacc tcaaactaaa agacttgaaa
     2881 aagaatttgg gtaatttgga gtcgtttttc catggaacaa gctctggaat agatccactt
     2941 agcagtattc tcaacgaacc aattttatgc aagaatgcca acgaaattgt taccacaaca
     3001 ttgcctttaa cagccccaga taaacaaaat gttattttct tattggatac caaacaagaa
     3061 cgtacaacta ccaaaatgat ggctttgttc aacgaattgt tgggaaaaga agaatttaaa
     3121 aacaagtttg ataaatacgt tatagaaaac aacaacggtg caattcaaag ttttttggac
     3181 aatgataaca aacgctttta tgaagttgtt aacgaactaa gtgcgttcca aataaactac
     3241 atgaaggact tttttcctga aagtttgcac aaagatgttc aaaaaggcct tgacaatggc
     3301 gattattttc taaaattgtg cggagcaggc ggtggtggtt ttgtacttgg ttttacgcaa
     3361 aattgggaac aaaccaaaga acaattgaac aactacgaat tagaagaaat ctacagattc
     3421 taacaaaaaa acggtggcat cgttaacaat accaccgcaa ctattcttct caacaaaagt
     3481 tgttaaaaca acccattaat gatcgtatcg tccaccattt ctggtacagt aacttttaaa
     3541 ttcggctcca tttccattgc acgtttgatt gcaaaaacag caccttcatt gcgcgcccaa
     3601 cttcttctag aaattccgtt gtttacatcc caatgcaaca ttgatttcaa acgacgttca
     3661 gaatcgctag aaccatctaa caacattcca aatccaccgt tgattacttc gccccaacca
     3721 acgccaccac cgttgtggat ggaaacccat gtagcacctc taaatgagtc accaataacg
     3781 ttgtgaattg ccatgtcagc agtaaagcga gaaccatcat aaatattgga ggtttctcta
     3841 aatggagaat ctgttccaga aacatcgtgg tggtctctac ctaagacaac cggaccaatt
     3901 tttccagcgg caatcgcatc gttgaaagct tttgcaattt ccatacgacc caaagcatcg
     3961 gcatataaaa tgcgtgcttg cgaaccaact accaatttat tggcttgcgc accttctatc
     4021 cattggatgt tatctttcat ttgctgctga atctctgctg gtgaatcttg tatcaagcgc
     4081 aacaatactt cttttgctaa ttcatcggtg atgtgcaaat cttctggttt tccagatgca
     4141 cacacccatc taaacggacc aaaaccgtag tcaaaacaca taggacccat gatgtcttgc
     4201 acatacgatg ggtatttaaa atcgatacca tttggcgcca tgatatccgc tccagcacgt
     4261 gatgcttctt tcaaaaaggc atttccgtaa tcgaagaaat aagtaccttt tgcagtgtgc
     4321 ccgttgatgg cttccacctg acggcgcaac gatgcttgca ctttttcttt aaacaattcg
     4381 ggattcgtag ccatcatttc gttggcttct tcatacgtta aaccaacagg atagtaacca
     4441 cccgcccacg gattgtgcaa cgatgtttgg tctgacccta aatcaatttg aatgttttgt
     4501 gctaagaaat actcccacac atccaccaca ttgccaacat acgctaatga aaccacttct
     4561 ttgtttgatt gtgcttttcg aacacgttct gccaacaagt gcacatcgtc aaacaattca
     4621 tctacccatc cttgactatg gcgtttttct gctgcattac ggttaacctc tgctgtaaca
     4681 gaaatacaac ctgcaatatt tccagctttg ggttgtgcac ccgacattcc tccaagtcct
     4741 gccgtaacaa acaagcctcc tttaattgtt tttccaattt ttctaaaacc attcaaaacg
     4801 gtgatggttg ttccgtgcac aattccttgc ggaccaatgt acatatatga accagcagtc
     4861 atttgtccgt attgtgttac tcccaacgca ttgaaacgct cccaatcatc gggttgtgaa
     4921 tagttgggaa tcatcattcc attggttact accacacgtg gagcatcttt gtgggatgga
     4981 aataaaccca tcggatggcc agaatacatt accaatgttt gttcatcggt catttctgcc
     5041 aaatatttca tagttaagcg gtactgaatc cagttttgaa aaacagcccc atttccacca
     5101 taggtaatca attcatgagg gtgctgagcc acagcataat ccaaattgtt ttgaatcatt
     5161 aacataatgg cttttgcttg taaactcttg ccaggatatt cgtcaataga acgggcaaat
     5221 agtttgtaat cagggcgata acgatacatg taaatgcgtc catattgatt caattcatcg
     5281 gcaaaatctt ttgccaactc agcgtgttgt tcttttggaa aataacgcaa ggcattgcga
     5341 acagctaatt ttttctcatc ttcacttaaa atagctttac gtttaggagc atgatttatg
     5401 ttcggttcgt aagctttttt tgcaggaatt tgtgctggaa caccttgtag aatatcgttg
     5461 aaaaattctt gttgcgtcat atttttgtaa ttttatttca caaagataag gtttatataa
     5521 aattagtttg tgcttcgatg ggtgaaaagt actaattgcg acaaaatttc agtttttaag
     5581 ctgtggcaca atgattgaaa tttataacag tactttaaaa atagtgcaaa gacagcaggt
     5641 tttttgaaaa tatctgccaa tttgtctaaa gatttataaa taatatgaac gaccaattaa
     5701 aagacgcttt aaagcaatta acagatatta ttgattcaga aacagaattc attccgctta
     5761 tcacttccga tgaggaggaa acaatgaaca atacatctta tccagatatt ttacccattt
     5821 taccattgcg caacaatgtg ctttttccag gagtggtaat tccaatcact gtggggagag
     5881 ataaaagcat taagttgatt caagcggcca acaagggtga taaaatagtg ggagttgtat
     5941 ctcaaaaaga ccaagaagat gaatcgccag aatttgatga tttacataaa attggaacgg
     6001 ttgctcaaat cattcgtttg ctaaaaatgc ctgatggaag ttcaacagcc atcattcaag
     6061 ggaaacgtcg ctttgagttg gttgaaccta ttcaaagtga gccgtttatc aaagcacgtg
     6121 tacagtattt ggaagaacca aaagttgatt acacttccaa agaaatgacc tttttgattg
     6181 attcggtgaa agaatcggct ttgcaaatca ttaaggagag ccccaacatt ccttccgaag
     6241 catcttttgc attaaaaaac atcgattctc cttcgttttt aatcaatttt gttagttcca
     6301 atatgaatgc cgatgtggca aaaaaacaat ccattttgga tgaattggac gctaaaaaac
     6361 gtttaatgtt ggtgctagag cagttgaatt tagaagtgca aatgttacaa atgcgcaatg
     6421 aaattcaatc caaagtaaaa aaagatatag atcgtcagca acgtgagtat tttttacacc
     6481 agcaaattcg tactattcaa gatgaattgg gtggaagtcc gcatgaaatg gatgtccaag
     6541 aattgcaaga gcgtgctttg cgtaaaaagt gggacgaaaa aacagaaaaa gtattttata
     6601 aagaattaga aaaacttaaa cgtaccaacc cacaaggagc cgaatacacg gtgttgctca
     6661 attatttgga tttattgttg gatttgcctt ggggcgttta tacaaaagac attcacgatt
     6721 taaaacgtgc tagtaaagtg ttggaacgcg atcactac
//
LOCUS       DAOQVQ010000080         6745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12463_length_6745_cov_4.165471, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000080 DAOQVQ010000000
VERSION     DAOQVQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12463_length_6745_cov_4.165471"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2627)
                     /locus_tag="PKN22_03775"
     CDS             complement(<1..2627)
                     /locus_tag="PKN22_03775"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS41856.1"
                     /translation="MIVLVTLTNGIFAQEHNHNHDLTPQEISCRNNVVSSFEKLKKNN
                     AFKQQFQSQFNADFNSFLEWNKYEVDYLLSYLKATVCYKDVVDFNAYVLESIERTQDN
                     YWFETPVLMHKPGQTQVGKYIYHKDEEPNVNKLDKDNPNEPKAACDNSDFEYGDYTNW
                     NRFCGTVTGAFMGATSGETTSCTGQHTLVTGGTDAYTGAPRVFQGSTSAMIGDGTGTG
                     ARYARLRRTFTVSSVDFSLTYNYMAVLQDPGTGHTNPERPFFNVKLFDAGGNEVNCAT
                     YFSYFGDGAPGWVTGGGVAYRTWTSVFVPLGAYNGQTLTFEITAGDCSASGHWGYAYV
                     DITCDQFTIDQFCQGTSTTLQAPTVGIQSYLWSTGETTPQITITAPGNYTCNVLPVGS
                     ICSATLNYTATIYPVPTASFTVAPNPTCVGGVVNLASTSTIDPGGTITQYQWNFGDGI
                     SSPSSSGTMTGVAQTTGTYTNPNHTYNSAGTSNITLNITTADGCTTSTQQPLTVVPGP
                     TATIAGTSTICNGTGSPAVTFTGGQTPGPYTFTYNINGGAAQTVTTSGGSSVVVPVPN
                     TPAGTYTYNLTHVSDPLSASCQSNVTDAATITINPTPTATIAGDAIVCQGDAAPTITF
                     TGANGTSTYQFEYSINGGAPQTINSVGSTATVTIPTGTPGTFAYSLISVTDVATGCSQ
                     TQAGTATVVVNPMPNATSTGATTVCQNAPSPQVTFTGSNSSNNYQFTYTLNGGANQTL
                     TTTGGANTATINVPTTVPGTFTYALTNVSDPATGCNQNLTQTQVVVVNPMPTATIAGT
                     TTVCQGSPAVNVTFTGANATPNYQFTYTLNGGATQTITTTGGNTATISIPTATPGTYT
                     YALQNVAQVTTGCSQPQTGT"
     gene            complement(2853..3818)
                     /locus_tag="PKN22_03780"
     CDS             complement(2853..3818)
                     /locus_tag="PKN22_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS41857.1"
                     /translation="MDFLPIYTKYIEAELSALNLPTTPQSLYEPISYFLKIGGKRIRP
                     ILTLLGAEMFGLKKEQAIHAAIAIELLHNFSLVHDDIMDKAPLRRGQETVHQKWNLNV
                     AILSGDAIFIKAYEHLQQQEGDLREILKTFNQAAIEICEGQQMDMDFESQEDISIDDY
                     IQMIRLKTSVLLGCALEMAAIIAGCDEQDRKHCYDFGVNIGLSFQIQDDILDLYGDPE
                     KFGKQIGGDVIENKKTILYLLAKKHANNKQLIELHSIKNELDNIRKIERTKILFETLN
                     VKQLAEDVANNYFNNALAHLHQIQVNIENKLHLSQLSNFLLNRNH"
     gene            complement(3818..4678)
                     /gene="rfbA"
                     /locus_tag="PKN22_03785"
     CDS             complement(3818..4678)
                     /gene="rfbA"
                     /locus_tag="PKN22_03785"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNS41858.1"
                     /translation="MKGIILAGGSGTRLHPLTLAISKQLMPIYDKPMIYYPLSILMEA
                     GIRDILIISTPHDMPLFQRLLGTGQHLGCNFHYKVQEEPNGLAQAFVIGEEFIGNDKV
                     ALILGDNIFYGAGMDEILKENNNPDGGVVYAYHVSDPQRYGVVEFDANMKAISIEEKP
                     AQPKSNYAVPGLYFYDNSVVEIAKNLKPSARGEYEITDVNAEYLRRNKLKVAILNRGT
                     AWLDTGTFSSLMQAGQFVQVIEERQGLKIGCIEEIAYKNGYITTEQLRILAEPLVKSG
                     YGTYLLNLLK"
     gene            4818..6656
                     /locus_tag="PKN22_03790"
     CDS             4818..6656
                     /locus_tag="PKN22_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41859.1"
                     /translation="MKHITKLTNKAIILSLSLGFIGLKPEIARSQTWSQTQKVVAQDR
                     IAFANFGNTVAIYGDYAFVGAPKDNTDASSQNAMDETGSVYIYRKVNNNWTFHQKIIA
                     SDRTNYDYFGWSVAVSGDYAVIGSYFSKTDATGANPLNGAGAAYIFKNNSGTWSQVQK
                     IVASDRNTQNYFGYAVDIDGDNIVIGAYQNSFDVSGGAQLSNAGAAYVFANNGTTWVQ
                     AQKIVASDRTMNDNFGKAVSISGKYLVVGAPQEDEDASNANYLAEAGSAYIYKENGGV
                     WSQIKKVVANDRSADANFGNAVDNTSDHVLIGAWKEKKDASGNNSITEAGAAYVYENN
                     SDAWSQQNKLVANDRFDVAHFGRSVSISNDKIVIGSEADNYNLNGHSSADSVTVAGSS
                     FIFQLQGNNWQQSQKLIANDRTEWANFGQSVAIFNNDILVGAYRDKFDANGLNSLNFA
                     GSAYFFGEDCLTDNSVSVNGETITATESGATYQWIDCDNSNTPIVGETSQAFTASVSG
                     NYAVEITKNGCTVTSACTAVNVASIQHKSGNVFTLSPNPTDGAFTITNSNANDVVIQL
                     YSLLGEEVYSATKKAGETIIVNISNQRAGIYLVKVNNSIVGKIVKR"
BASE COUNT         2054 a         1159 c         1251 g         2281 t
ORIGIN      
        1 gtacctgttt gtggctgtga acatcctgtg gttacttgcg caacattttg aagggcatac
       61 gtgtatgttc caggtgttgc cgttggaatt gaaatggttg ccgtgtttcc acctgttgtt
      121 gttattgttt gtgttgcacc accattcaat gtatatgtaa attgataatt tggtgttgcg
      181 tttgctcctg taaatgtaac gttaactgca ggtgaacctt gacaaacagt tgttgttcct
      241 gcaatagttg cagttggcat tgggttaaca accaccactt gtgtttgtgt caagttttgg
      301 ttacatcccg ttgctgggtc agatacattg gttaaagcat aagtaaacgt acccggaact
      361 gttgtaggaa cattgattgt tgctgtgttt gctccacctg ttgttgttag tgtttggttt
      421 gcaccaccat tcaaagtata agtaaactga tagttattgc tactattgct tcctgtaaaa
      481 gtaacttgtg gagaaggtgc attttgacaa accgttgttg cacctgtaga tgttgcattt
      541 ggcatcggat ttaccacaac agttgctgtt cctgcttgag tttgtgaaca tcctgttgca
      601 acatctgtta ctgaaattaa actataagca aaagttccag gtgttccagt tggaatggta
      661 actgttgctg ttgagcctac agaattgatt gtttgtggcg ctcctccatt aatagaatat
      721 tcaaattgat aagtagatgt accatttgca ccagtaaacg taattgttgg agcagcatca
      781 ccttgacata ctattgcatc tcctgcaatg gttgcagttg gagttgggtt gattgttatc
      841 gttgctgcat cagtaacatt tgattgacat gaagcggaaa gtggatcaga tacatgggta
      901 agattgtagg tataggtacc cgctggagta tttggaacag gaactactac actgctacct
      961 cctgatgttg ttaccgtttg cgcagcacca ccattaatat tgtatgtaaa tgtataaggt
     1021 ccaggtgttt gtcctcctgt aaacgtaact gctggtgaac ctgtaccgtt acaaatagta
     1081 gaagtacctg caatagtggc tgtcggacct ggcacaaccg taagtggttg ctgagttgag
     1141 gttgtacaac catctgcagt ggtaatgttt aaagtaatgt tgcttgttcc tgctgaatta
     1201 taggtatgat ttggattggt gtatgtacca gttgtttgcg caactcctgt cattgttcca
     1261 gaagaacttg gagaactaat gccatcgcca aaattccatt ggtattgcgt tatggttcca
     1321 cctggatcaa tggttgaagt acttgcaagg ttaaccactc ctcccacaca agttggattt
     1381 ggagctacag taaaactagc tgtaggcact gggtaaattg ttgcagtata gttcagtgtt
     1441 gcagaacaaa tagacccaac aggtaataca ttacaagtgt aattccctgg tgcggtgata
     1501 gtaatttgag gggttgtttc acctgtactc cataaataag attgaatacc aactgttggt
     1561 gcttgaagtg tagttgaagt tccttgacaa aattggtcaa ttgtaaattg atcacaggtt
     1621 atatccacat aagcatatcc ccagtgtccc gaagccgaac agtctccagc tgtaatttca
     1681 aaagtcaacg tttgaccatt ataagctcct aaaggcacaa aaacagatgt ccaagttcta
     1741 taagcaactc caccacctgt tacccatcca ggcgcaccat caccaaaata agagaaataa
     1801 gttgcacagt taacttcatt cccccctgca tcaaataatt tcacattgaa aaacggacgt
     1861 tctggattag tatgccctgt acctggatct tgcaatactg ccatgtaatt ataagttaat
     1921 gagaaatcaa cactagaaac agtaaaggtt cttcttaaac gtgcatatct agctcctgta
     1981 ccagtaccat caccaatcat ggcagaggta gaaccttgaa aaactctagg agcgccagta
     2041 taagcatctg ttccaccagt aacaagtgta tgttgaccag tacaagaagt agtttcacct
     2101 gatgttgctc ccataaaagc tcctgttaca gttccacaaa aacggttcca gttggtgtaa
     2161 tcaccgtatt caaaatctga gttatcgcac gctgctttgg gttcatttgg attgtcttta
     2221 tccaacttat ttacatttgg ttcttcatct ttatgataaa tgtattttcc aacctgagtt
     2281 tgccctggct tatgcattaa tacaggcgtt tcaaaccaat aattatcttg cgttctttca
     2341 attgattcaa gcacataggc attgaaatca acaacgtctt tataacaaac tgttgctttt
     2401 aaatacgata atagataatc aacttcgtat ttattccact ccaaaaatga gttaaaatca
     2461 gcattaaact gactttggaa ttgttgttta aatgcattgt tcttctttaa tttttcaaaa
     2521 gaactcacca cattgtttct acaactaatt tcttgaggtg ttaaatcatg gttatgattg
     2581 tgctcttgag caaatatccc atttgtaaga gtcaccaata ctatcatagt caaaaaaata
     2641 gttctgacag atagtagttt taagtttcta aagtgagtag ccataatcat aagagttatt
     2701 ttgagttaaa aatataaaat tttaaacttt attttaacgt attacgaaaa tattttttat
     2761 aagttgccta aaatcagcta ttttattgat tttttaagac caaatcattc aaaaaccagt
     2821 actaaaccct tttatttgtc gatgagattt ttttaatgat ttctgtttaa taagaaatta
     2881 ctcaattgcg ataagtgcaa tttattttca atatttactt gaatttgatg caagtgcgca
     2941 agtgcattgt tgaaataatt atttgctaca tcttctgcaa gttgtttcac atttaatgtt
     3001 tcaaataata ttttagttct ttcaattttt ctgatgttat ctaattcatt tttaatagaa
     3061 tgaagttcaa ttaattgttt gttgttggca tgtttttttg ctaataaata aagaattgtt
     3121 tttttatttt caataacatc acccccaatt tgttttccaa atttttctgg atctccgtat
     3181 aaatcaagaa tatcgtcttg aatttgaaat gaaagtccta tattaacacc aaagtcataa
     3241 caatgtttcc tgtcttgttc gtcacatcct gcaataattg cagccatttc aagtgcacaa
     3301 cctaataaaa cagatgtttt tagacgtatc atttggatat aatcgtcaat agaaatgtct
     3361 tcttgcgatt caaaatccat gtccatttgc tgtccttcac atatttcgat ggctgcttgg
     3421 ttgaatgttt ttaatatttc tcttaaatcg ccctcttgtt gttgtaggtg ttcataagct
     3481 ttaataaaaa ttgcatcgcc agaaagaata gctacgttta aattccattt ttgatgaacc
     3541 gtttcttgcc ctcttctcaa aggagctttg tccataatat catcgtgcac caacgagaaa
     3601 ttgtgtaata attcaatcgc aattgcggca tgaatagctt gttctttttt tagcccaaac
     3661 atttctgccc ccaacaatgt taaaataggt cgaattcttt tacctccaat ttttaagaaa
     3721 taggatattg gttcgtataa tgattgaggt gttgttggta aatttaatgc agaaagttct
     3781 gcttcaatgt attttgtata gatgggtaaa aaatccatta ttttaataaa tttaaaagat
     3841 atgtgccata accacttttc accaatggct cggccaatat acgcaattgt tcggttgtaa
     3901 tgtatccatt tttgtaggca atttcttcga tacagccaat ttttaatcct tgtcgttctt
     3961 caattacttg tacaaactga cctgcttgca tcaacgatga aaacgtacct gtatccagcc
     4021 aagctgttcc tctatttaaa atcgcaactt ttaatttatt tctacgcaaa tattcagcgt
     4081 taacatctgt aatttcatat tctccgcgag cggatggttt taaattttta gcaatttcta
     4141 ctaccgaatt atcgtaaaaa tacaatccag gtactgcata atttgatttt ggttgtgcgg
     4201 gtttctcctc gatagaaata gctttcatgt tagcatcaaa ttcaacaact ccataacgtt
     4261 gtggatcgct cacatgatat gcataaacaa ctcctccatc aggattgttg ttttctttta
     4321 aaatttcatc cattccggca ccataaaaaa tattatcccc taagatgagt gctactttat
     4381 cgttgccaat aaattcttca ccaataacaa atgcttgtgc caaaccattt ggttcttctt
     4441 gcactttgta gtgaaaatta catcctaaat gctgccctgt tcctaacaat ctttgaaata
     4501 aaggcatatc gtgcggtgta gagatgatta aaatatcacg aattccggct tccattagta
     4561 tagataatgg ataataaatc attggtttat cgtaaattgg catcaattgt ttactgatgg
     4621 ccaatgttaa tgggtgcaaa cgtgttcctg agcctcctgc taaaataatc cctttcatat
     4681 tgatggttga ggtttaaata ttttctacaa taatatttat tttaattcaa ttatttgaat
     4741 ttttttaacg catcattgca tttaattaac agaaaaatgc ttatacttgg gtttataaac
     4801 atttaaatac tacactcatg aaacacatca caaaacttac aaacaaagca ataatccttt
     4861 cattatcatt ggggtttatt ggattgaaac ctgaaattgc acgttcgcaa acatggtcgc
     4921 aaacgcaaaa agttgttgct caggatagaa tagcttttgc aaattttgga aatacagttg
     4981 caatttatgg cgactatgct tttgttggcg ctccaaaaga caatacagat gctagttcgc
     5041 aaaatgcaat ggatgaaaca ggctcggttt acatctatag aaaagtcaac aataactgga
     5101 catttcatca aaaaataatt gcttcagata gaacaaatta tgactatttt ggatggtcgg
     5161 ttgctgtttc gggtgattat gcagttatag gctcttattt ttctaaaaca gatgcaacgg
     5221 gtgccaatcc gttgaatgga gcaggtgcag cctacatttt taaaaacaat tcaggcacat
     5281 ggtcacaggt tcaaaaaatt gttgcttctg atagaaatac tcaaaattat tttggctatg
     5341 cggttgatat agatggagat aacattgtta ttggagctta tcaaaacagt tttgatgtta
     5401 gcgggggcgc acagttatct aatgcaggtg cggcttatgt gtttgcaaat aatggaacaa
     5461 cttgggtgca agcacaaaaa attgttgctt cagacagaac aatgaatgat aattttggta
     5521 aagcagtttc tatttctgga aaatatttgg ttgtaggagc tccacaagaa gatgaagatg
     5581 caagcaatgc aaattattta gctgaagctg gatcggctta tatttataaa gaaaatggtg
     5641 gtgtttggtc tcaaatcaaa aaagttgttg ctaatgatag gagtgccgat gcaaattttg
     5701 gaaatgcagt tgacaacaca tctgaccatg ttttaattgg cgcatggaaa gaaaaaaagg
     5761 atgcaagtgg taataattca attacagagg cgggtgctgc ttatgtttat gaaaacaatt
     5821 ccgacgcatg gtcacaacaa aataaattgg tagcaaacga tagatttgat gttgctcatt
     5881 ttggaagaag tgtttccatt agtaatgata aaattgtaat tggctctgag gctgataatt
     5941 ataatttgaa tggacatagt tctgctgata gcgtgacagt tgcagggtca tctttcattt
     6001 tccaattgca aggaaataat tggcaacaat ctcaaaaatt aattgcaaac gatagaacag
     6061 agtgggccaa ctttggacaa tctgttgcta tttttaataa cgatattttg gtaggagctt
     6121 atcgtgataa atttgatgcg aatggtttaa attctttaaa ttttgcaggc tctgcctact
     6181 tttttggtga agattgttta acagacaata gtgtgagtgt gaatggtgaa accattacag
     6241 caacagaaag tggagctacc tatcaatgga tagattgcga taattcaaac actccaattg
     6301 taggagaaac ttcacaagcg tttacagctt ctgtttctgg aaattatgca gttgaaatta
     6361 caaaaaatgg ttgtacggta acgtctgcat gtactgcagt aaatgttgcg agtattcaac
     6421 ataaaagtgg taatgtgttt actctttcac ccaatccaac agatggtgct tttacaatta
     6481 ccaattcaaa tgcaaacgat gttgttattc aattgtattc tttattgggt gaagaagttt
     6541 attctgccac caaaaaagcg ggtgaaacaa ttattgtaaa tatcagtaat caacgtgcag
     6601 gtatttattt agtgaaagtc aataattcaa ttgttggaaa aattgtgaaa cgataaacaa
     6661 cattcatttt tttcaaaaaa ggagtcttcg gacacgaatg gtcggaagaa atttcttccg
     6721 acttttttgt atttattaac cttta
//
LOCUS       DAOQVQ010000081         6703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12555_length_6703_cov_3.401775, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000081 DAOQVQ010000000
VERSION     DAOQVQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_6703_cov_3.401775"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="PKN22_03795"
     tRNA            1..74
                     /locus_tag="PKN22_03795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Thr,seq:tgt)
     gene            92..164
                     /locus_tag="PKN22_03800"
     tRNA            92..164
                     /locus_tag="PKN22_03800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Gly,seq:tcc)
     gene            187..258
                     /locus_tag="PKN22_03805"
     tRNA            187..258
                     /locus_tag="PKN22_03805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Thr,seq:ggt)
     gene            359..1546
                     /gene="tuf"
                     /locus_tag="PKN22_03810"
     CDS             359..1546
                     /gene="tuf"
                     /locus_tag="PKN22_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNS41860.1"
                     /translation="MAKENFDRSKPHVNIGTIGHVDHGKTTLTAAISSVLANQGLAEK
                     RDFSSIDNAPEEKERGITINTSHVEYQTKNRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPRMVVFMNKVDMVDDAELLELVEMEIRELLSF
                     YDYDGDNTPVIKGSALGALNGEEQWVNTVNELMDAVDSWIELPKRDVDKPFLMPIEDV
                     FTITGRGTVATGRIETGVINSGEAVDILGMGEEKLSSTVTGVEMFRKILDRGEAGDNV
                     GLLLRGIDKSQIRRGMVICKPGSTKPHKTFKAEIYVLKKEEGGRHTPFHNKYRPQFYF
                     RTTDVTGEIILTDGREMVMPGDNVTIQVNLIVPVAMDKGLRFAIREGGRTVGAGQVTD
                     IVE"
     gene            1592..1664
                     /locus_tag="PKN22_03815"
     tRNA            1592..1664
                     /locus_tag="PKN22_03815"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1625..1627,aa:Trp,seq:cca)
     gene            1686..1907
                     /gene="secE"
                     /locus_tag="PKN22_03820"
     CDS             1686..1907
                     /gene="secE"
                     /locus_tag="PKN22_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNS41861.1"
                     /translation="MANFINYINETIEELVHRVSWPTWKELQNNTIIVVVASILISLI
                     IFVMDYVAGIVPSNGGFWKGVLGFIYSGF"
     gene            1921..2472
                     /gene="nusG"
                     /locus_tag="PKN22_03825"
     CDS             1921..2472
                     /gene="nusG"
                     /locus_tag="PKN22_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNS41862.1"
                     /translation="MSEIKKLWYVVRAISGKEKKVKEYLELEISRMKLDDYIGQVLIP
                     TEKVFQMKNGKKVSKEKAYLPGYVLVEAALVGEVSHVIKGIPNVIGFLGAEKGGEPVP
                     MRLSEVNRILGKVDELLETEEEMKIPFIVGESVKVIDGPFNNFSGTNEEINEEKKKLK
                     VMVKIFGRKNLLELSYLQVEKEV"
     gene            2554..3003
                     /gene="rplK"
                     /locus_tag="PKN22_03830"
     CDS             2554..3003
                     /gene="rplK"
                     /locus_tag="PKN22_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNS41863.1"
                     /translation="MAKEVSGLIKLQIKGGAANPAPPVGPALGSKGVNIMEFCKQFNA
                     RTQDKAGKVVPVVITVYSDKSFDFILKTAPAASQILEIGKFKKGSGEPNRSKVGSLTW
                     DQVRTIAEDKLADLNCFTVDAAMTMVAGTARSMGVTVKGGEEPKSKN"
     gene            3009..3704
                     /gene="rplA"
                     /locus_tag="PKN22_03835"
     CDS             3009..3704
                     /gene="rplA"
                     /locus_tag="PKN22_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNS41864.1"
                     /translation="MARVSKKRKEILAKYDFTKSYSLAEASKLVREISTTKFDASIDV
                     SVRLGVDPRKANQMVRGTVALPHGTGKDVRVLVLCTPDKEAEAKAAGADHVGLDDYIE
                     KIKGGWTDIDVIITLPSVMGKVGALGRVLGPRGLMPNPKTGTVTMDVAKAVEEVKAGK
                     IDFKVDKFGNIHAGIARASFDGDKIKDNVNELIQTLIKLKPSAAKGTYIKSIFLSATM
                     SPSVQVDTKSVVA"
     gene            3717..4238
                     /gene="rplJ"
                     /locus_tag="PKN22_03840"
     CDS             3717..4238
                     /gene="rplJ"
                     /locus_tag="PKN22_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNS41865.1"
                     /translation="MNREEKAKYIDDLAAQLNEANVVYLADTADLTVEVINQLRRKCF
                     QNNISIRVVKNTLLAKAIEKVDSKNFGDLPTVLAGSTSIMFSEVGNAPAKLIKDFRAK
                     NAKPILKGAYIEEAVFIGDNQLDALVAIKSKEEVIGDIIGLLQSPAKNVISALKGQGG
                     KIAGILKTLEERA"
     gene            4284..4664
                     /gene="rplL"
                     /locus_tag="PKN22_03845"
     CDS             4284..4664
                     /gene="rplL"
                     /locus_tag="PKN22_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNS41866.1"
                     /translation="MADLKQIAETLVNLTVKEVNELAAILKDEYGIEPAAAAVAVAAV
                     GGGAGAGEAAAEQTEFTVILKSAGANKLAVVKLVKELTGQGLKESKDIVDGAPATLKE
                     GVSKDEAEGLKKSLEEAGAEVEIK"
     gene            4772..>6703
                     /gene="rpoB"
                     /locus_tag="PKN22_03850"
     CDS             4772..>6703
                     /gene="rpoB"
                     /locus_tag="PKN22_03850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNS41867.1"
                     /translation="MATNNKTTRISFATGKAQFDYPDFLDIQLKSFQEFFQLETTPEN
                     RESEGLYKVFAENFPISDTRNQFVLEFLDYFIDPPRYTIEECIERGLTYSVPLKAKLK
                     LYCTDPEHEDFETIVQDVYLGTIPYMTPKGSFVVNGAERVIVSQLHRSPGVFFGQSRH
                     ANGVKLYSARVIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLFRAIGYESDKDILE
                     IFGLADEVKVSKAALKKIVGRQLAARVLKSWVEDFVDEDTGEVVSIERNEILLDRETF
                     LEEEHIDLIVDAGVKTIILHKENANSADYGIIYNTLQKDISNSEKEAVEHIYRQLRNA
                     EPPDYETAKSVFEKLFFSDTRYDLGEVGRFRLNKKLGIDNSEESRVLTKEDIILIIKY
                     LIELINSKADVDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTP
                     IDLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEVTHKRRLSALGPGGLSRDRAGFEV
                     RDVHYTHYGRLCTIETPEGPNIGLISSLCVFAKINKLGFIETPYRNVENGVVNLKDEP
                     VYLTAEEEEAKTIAQANAKIDDKGNFLTEYVKARYEGDFPVVNPTELSLMDVGANQIA
                     SIAASSIPFLENDDANRALM"
BASE COUNT         2219 a         1052 c         1461 g         1971 t
ORIGIN      
        1 gccgatgtag ctcagttggc tagagcagct gatttgtaat cagcaggtcg tgggttcgaa
       61 tccctccatc ggctcatttt taaatatttt cgcgggagta gctcagttgg tagagcgtca
      121 gccttccaag ctgagggtcg cgagttcgaa tctcgtctcc cgctcaaata aataaaagag
      181 ctttttgccg gtgtagctca ggggtagagc gcttccttgg taaggaagag gtcacgagtt
      241 caaatctcgt cattggctcg aaaagttagt tgtgacttta attttcactc agagatgggt
      301 gaaatttagt gtatatataa atttgtattt ttattaacta agtaaacaag aataaaagat
      361 ggctaaggaa aatttcgacc gttccaaacc acacgtaaac attggaacaa ttggtcacgt
      421 tgaccacgga aaaacaactt tgacagcagc tatttcatca gtattagcta atcaaggttt
      481 ggcagagaaa cgcgatttct cttctattga taacgctcca gaagaaaaag agcgtggtat
      541 tacaatcaac acttcacacg ttgagtacca aacaaaaaat cgtcactatg cacacgttga
      601 ctgtccagga cacgccgact acgttaaaaa catggttact ggtgctgcgc aaatggacgg
      661 agctatcttg gttgtagctg caacagacgg accaatgcct caaactcgtg agcacatcct
      721 tttaggtcgc caagttggtg ttccaagaat ggttgtattc atgaataaag tggatatggt
      781 tgacgacgct gagttgttag aattagttga aatggaaatc agagagttgc tttctttcta
      841 cgattacgat ggtgacaata ctccagttat caaaggttct gctttaggag ctttgaacgg
      901 tgaagagcaa tgggtaaaca cagtaaacga attgatggat gcagtagatt catggattga
      961 attaccaaaa cgtgatgttg ataaaccttt cttgatgcca atcgaagacg ttttcacaat
     1021 tacaggtcgt ggaacagttg ctacaggacg tatcgaaaca ggtgtaatca actcaggtga
     1081 agctgttgat atcttaggta tgggtgaaga aaaattatct tctactgtaa ctggggttga
     1141 gatgttccgt aaaatcttgg atagaggtga agctggtgac aacgtaggtt tattgttacg
     1201 tggtatcgac aagtctcaaa tccgtcgtgg tatggttatc tgtaaaccag gttcaacaaa
     1261 accacacaaa actttcaaag ctgagattta cgttttgaaa aaagaagaag gtggacgtca
     1321 cactccattc cacaacaaat accgtcctca attctatttc cgtacaactg acgttacagg
     1381 tgaaatcatc ttaactgacg gacgtgaaat ggttatgcca ggtgataacg ttacaatcca
     1441 agttaacttg atcgtaccag ttgcgatgga caaaggatta cgtttcgcta tccgtgaagg
     1501 tggtagaact gtaggtgcgg gtcaggtaac tgatatcgta gaataataaa tactttaaga
     1561 ggaagttttt taagcttcct cttatttata cacgggctta gctcagctgg tagagtagtg
     1621 gtctccaaaa ccattggtcg tgggttcgaa tcctacagcc cgtgcaaact taaaaaaaca
     1681 ttaaagtggc aaattttatt aactatatca acgaaactat tgaagagtta gtccacagag
     1741 tttcttggcc tacttggaaa gaattgcaaa acaatactat cattgtagtt gtagcatcta
     1801 ttttgatttc attaattata tttgttatgg actatgttgc tggtatcgtg ccatctaatg
     1861 gaggattctg gaaaggagtt cttggtttca tttatagtgg attttaattt gtgattagtg
     1921 atgagtgaaa tcaaaaaatt atggtatgta gttagagcca ttagcggcaa agagaaaaaa
     1981 gtgaaagaat atcttgaact tgaaatctct cgcatgaaat tggacgatta catcgggcaa
     2041 gtattgattc ctactgaaaa agttttccaa atgaaaaatg gaaaaaaagt aagtaaggaa
     2101 aaagcatatt taccgggata cgttttagta gaagctgcac ttgttggtga ggtttctcac
     2161 gttattaaag gtattcctaa tgttattggc ttcttagggg cagaaaaagg tggagaacct
     2221 gttccaatgc gtctttcaga agtgaataga attttaggaa aagttgatga acttttagaa
     2281 actgaagaag aaatgaaaat tccatttatt gttggagaat ctgttaaggt tattgacggt
     2341 ccattcaaca acttctctgg tacaaatgaa gaaatcaacg aagaaaagaa aaaactgaaa
     2401 gtaatggtga agatttttgg aagaaaaaat cttttggaat taagttactt acaggtagaa
     2461 aaagaagtgt aaatttttaa ccgtaggagt gagttagacg aataaagctt cccgtcgctt
     2521 gagctcatgt ttgactacat aatttaaata caaatggcaa aagaagtatc aggcttgatt
     2581 aaattgcaaa tcaaaggtgg tgctgctaat cctgcacctc cagtaggacc tgctttgggt
     2641 tctaaagggg tgaacatcat ggagttctgt aagcagttta atgctagaac acaagataaa
     2701 gcaggaaaag ttgttccagt tgttatcact gtgtacagtg ataaatcttt tgattttatt
     2761 ctaaaaacag ctccagctgc ttcgcaaatc ttggaaattg gtaaattcaa aaaaggatct
     2821 ggtgagccta accgctctaa agtaggttct ttaacttggg accaagttcg tacgattgca
     2881 gaagataagc tagcagattt gaactgtttt actgttgacg cagctatgac tatggttgca
     2941 ggtactgcac gtagtatggg tgttacagtt aaaggtggag aagaacctaa atctaaaaat
     3001 taatagttat ggcaagagta tctaagaaaa gaaaggagat tttggcgaaa tacgatttca
     3061 caaaatcata cagcttggca gaagctagta aattagttag agaaatctct acaactaagt
     3121 ttgatgcatc tattgatgtt tcagttcgtt taggtgttga ccctcgtaaa gcaaaccaaa
     3181 tggtgcgtgg aacggtggca ttgcctcatg gtactggtaa agatgttcgt gtattggttt
     3241 tatgtactcc tgacaaagaa gcggaagcta aagcggcagg tgcagatcac gtaggtttgg
     3301 acgattatat cgaaaaaatc aaaggtggtt ggactgatat cgatgttatc attacattac
     3361 cttctgttat gggtaaagtt ggtgctttag gtcgtgtttt aggaccaaga ggattgatgc
     3421 ctaaccctaa aacaggaact gtaacaatgg atgttgcaaa agctgtggaa gaagtaaaag
     3481 cgggtaaaat tgatttcaaa gttgataaat ttggtaatat ccacgcaggt atagcaagag
     3541 ctagctttga cggagataaa atcaaagaca acgtgaatga attaattcaa acgcttatta
     3601 aattaaaacc gtctgctgca aaagggactt atatcaaaag tattttcttg agtgctacaa
     3661 tgagcccaag tgtacaagtt gatacaaagt ctgttgttgc ttaaaactga tttataatga
     3721 atagagaaga aaaagcaaag tatatagacg atttagcagc tcaattaaat gaggcaaatg
     3781 ttgtctatct ggcagataca gcagatttaa ctgtagaagt aatcaatcag ttgagaagaa
     3841 aatgttttca aaataacatc agtattcgtg tagtaaagaa tacattgtta gcgaaagcaa
     3901 ttgaaaaagt tgatagcaaa aactttggtg atttaccaac tgttttggca gggtcaactt
     3961 ctatcatgtt ctcagaagta ggtaacgctc ctgctaaatt aattaaagac tttagagcta
     4021 aaaatgcgaa accaatttta aaaggtgctt atattgaaga agcagttttt attggtgaca
     4081 atcaattaga tgctttagtt gcaattaaaa gtaaggaaga agttattggt gatatcatcg
     4141 gattgcttca aagtcctgct aaaaatgtta tctctgctct taaagggcaa ggaggtaaga
     4201 ttgccggaat ccttaaaacg ctcgaagaaa gagcataata ttttagaaat aattattttt
     4261 gaaccttttt aaattttaat aaaatggcag atttaaaaca aatcgcggaa acattagtta
     4321 acttaacagt taaagaagta aatgaattag ctgcaatctt aaaagatgag tatggtatcg
     4381 agccagcagc agcagcagta gcagtagcag cagtaggtgg tggtgctgga gcaggagaag
     4441 cagcagcaga acaaactgag ttcactgtaa tcttgaaatc agcaggagct aacaaattag
     4501 ctgttgttaa attggtgaaa gaattgacag gtcaaggatt gaaagaatct aaagacatcg
     4561 ttgatggtgc acctgcaaca ttgaaagaag gcgtttctaa agacgaggct gaaggtctta
     4621 aaaaatcttt agaagaagct ggagctgaag ttgaaattaa gtaattttac tttatactaa
     4681 ggaaggcgtc ccgaattttc gggatgccta ttcctgtttt taggcctaaa cacgctagaa
     4741 tgcccataat cataaacaaa aacgtttgac attggcaaca aataataaga caacaagaat
     4801 aagctttgca acgggtaaag cacagtttga ttatccagat ttcttggata ttcaattaaa
     4861 atcctttcaa gaatttttcc agttagaaac tactccggaa aatcgtgaaa gtgaaggatt
     4921 gtataaagtt tttgctgaaa actttccaat atctgatacg agaaatcaat tcgtgttgga
     4981 gtttttggat tacttcatcg atccaccaag atacaccatc gaagagtgta ttgaaagagg
     5041 tttaacttat agtgtgccgt taaaggctaa attaaaactg tattgtacag accctgaaca
     5101 cgaagatttt gaaactatcg tacaggatgt gtatttggga actatcccat acatgactcc
     5161 aaaaggttca tttgttgtaa atggtgctga acgtgttatc gtttctcaat tgcaccgttc
     5221 tccaggtgtt ttctttggac aaagtcgcca tgcaaatggt gttaagttgt attcagctcg
     5281 tgtaattcct tttaaaggat catggattga gtttgcaacg gatattaata atgtaatgta
     5341 cgcttatatt gatcgtaaga aaaagttgcc tgttacaact cttttccgtg caatcggtta
     5401 tgaaagcgat aaagatattt tagaaatttt cggattggct gatgaagtga aagtttcaaa
     5461 agctgctttg aaaaaaatag taggccgtca attggctgct cgtgtgttaa aatcatgggt
     5521 tgaagatttc gtagatgaag atacaggaga agttgtatct atcgaacgta acgaaatttt
     5581 attagatcgt gaaactttcc ttgaagaaga acacattgac ttaattgttg atgcgggtgt
     5641 taaaacaatt attcttcata aagaaaatgc caactctgct gattatggca ttatttacaa
     5701 cacacttcaa aaagatattt ccaactctga aaaagaggct gtagaacata tttaccgtca
     5761 attgcgtaac gctgaaccac ctgattatga aacagcaaaa tctgtatttg agaaattatt
     5821 cttctcagat acacgctatg atttaggtga agtaggtcgt ttcagattga ataaaaaatt
     5881 aggaattgat aattctgaag aatcacgcgt tttaacgaaa gaagatatca ttttaatcat
     5941 taaatatttg attgaattga ttaactcaaa agcggatgtg gatgatattg accacttgtc
     6001 aaaccgtcgt gttagaacgg taggtgaaca attatatgct caatttggtg ttggtttggc
     6061 tcgtatggca agaactatcc gtgagcgtat gaacgtgcgc gataatgagg tctttactcc
     6121 aattgacttg attaacgcta aaacactttc ttctgttatc aactcattct ttggtactaa
     6181 ccagttatct cagttcatgg accaaacaaa cccattggca gaggtaactc acaaacgtcg
     6241 tttgtcggca ttaggacctg gtggactttc tcgcgatcgt gcagggtttg aggtgcgtga
     6301 cgttcactat acacactacg gacgtttgtg tacaattgaa acaccagagg gaccaaacat
     6361 cggtttgatt tcttcacttt gtgtgtttgc aaaaatcaat aaattaggtt tcattgaaac
     6421 gccatataga aatgtagaaa atggtgtggt gaatttaaaa gatgaaccag tttatttaac
     6481 agcagaagaa gaagaagcaa aaactattgc acaagctaat gctaagattg acgataaggg
     6541 taacttctta acggagtatg taaaagcacg atatgaagga gatttccctg tagttaatcc
     6601 tacagaatta agcttgatgg acgtaggtgc aaaccaaatt gcatccattg cggcatcttc
     6661 tattcctttc ttggaaaatg acgacgccaa ccgtgcattg atg
//
LOCUS       DAOQVQ010000082         6621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12758_length_6621_cov_4.571276, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000082 DAOQVQ010000000
VERSION     DAOQVQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12758_length_6621_cov_4.571276"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKN22_03855"
     CDS             complement(<1..370)
                     /locus_tag="PKN22_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS41868.1"
                     /translation="MKFKEYNINKAIKDQLEEMGFVRATDIQYKAIKPILDGEDVMAI
                     AQTGTGKTAAFAIPTLHFLAQKSKYTKGNVRCLVMVPTRELAQQIAEVFQTIGKKTSV
                     KVMGLFGGVEQDAQIKQLKSG"
     gene            512..775
                     /locus_tag="PKN22_03860"
     CDS             512..775
                     /locus_tag="PKN22_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41869.1"
                     /translation="MKKQFLILTLTVAFVFLVDLAFGQGCSQCKMVSEQSGEAGEAAF
                     ASNINIGILFLMAMPYIIVFAVFRKKLFAFFKSFFFTPNQSSK"
     gene            788..1351
                     /gene="pth"
                     /locus_tag="PKN22_03865"
     CDS             788..1351
                     /gene="pth"
                     /locus_tag="PKN22_03865"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNS41870.1"
                     /translation="MKYLIVGLGNPGSEYAETRHNIGFKVLDALAKESAATFKSDKLA
                     TCTEVKFKGRTLVLVKPTTYMNLSGKAVNYWMQAHKIPLENVLVITDDIALPFGKLRM
                     KGKGSDGGHNGLKDIQAQLKTQEYARLRFGVGSDFNKGRQVDYVLGEWSAEEKQTIDE
                     RIALAIEFIKGFATIGLNQTMSNLNNK"
     gene            complement(1354..3696)
                     /gene="topA"
                     /locus_tag="PKN22_03870"
     CDS             complement(1354..3696)
                     /gene="topA"
                     /locus_tag="PKN22_03870"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNS41871.1"
                     /translation="MAKNLVIVESPAKAKTIEGYLGKDFIVKSSFGHVRDLAKKGLAI
                     DIENNFTPSYEVSSDKKTVVSDLKKLVKEVDVVWLATDEDREGEAISWHLYEALNLSK
                     KETKRITFNEITKPAILKAIENPRQINQELVNAQQARRVLDRIVGFELSPVLWKKVRP
                     SLSAGRVQSVAVKLIVEKEREIEKFSVNSFYRVNGNFTTKNGKIKSELSTQLKSEKEA
                     ETLLENCKDAQFKVADVQKKPATKKPAAPFTTSTLQQEASLKLGFSVSRTMTVAQKLY
                     ESGFITYMRTDSVNLSDTAIEGAKNEVVKSYGASYSNPTKYKTASSGAQEAHEAIRPT
                     NFAAHSIDLGKDEDRLYDLIWKRAIASQMSHAELERTTITIETDKIAQHFTAKGEVIK
                     FDGFLRVYIESSLDDEEDENTEGLLPSVEVGELLKTDSIIATQRFTRPPSRYVEASLV
                     KKLEELGIGRPSTYAPTISTIQKRGYVEKRELEGEERKYTVYELKNGSIQSVVKTETT
                     GKEKNKLSPTDIGLVVTDFLEEHFPNIMDYHFTADVEEQFDKIAQGLKEWTKMIHEFY
                     NPFHSHVTDTLEHSERATGERELGVHPTSGKKIIARIGRFGPMIQIGDEQEDGEKPQF
                     ASLRREQSIHSITLEEAIDLFKLPRSLGEHDGLVVKANVGRFGPYIQHGKVFASIPKG
                     EDPMTITFERAVEILVAKQEADANKIIKTFAEDETMQLLNGRFGAYLKIGKDNFKLPK
                     GTEPEKLTYEDCVEISKNQKSTGTKAKASAKAKPKATKKK"
     gene            4091..6235
                     /locus_tag="PKN22_03875"
     CDS             4091..6235
                     /locus_tag="PKN22_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNS41872.1"
                     /translation="MKRILHVIGVVLAGTFVANAQQFSCATDHYYHQLVQQDPSIVKD
                     LENLIANGVAEKADEDSTTIFTIPVVFHVLHQNGAENIPDANIIDQINILNRDYMTLN
                     ADTSLVIPQFQGKIGKAKIQFKLAAYDPWGNCTNGIDRIYTHETSNGGAYSKVNQWDR
                     SKYLNIWVVKAMPEVSGAAGYALYPTAVNGYAYWMDGIMILYNYVGSLSPSSVYNSRA
                     ITHEVGHWLALPHVWGDNNDPGVACGDDGIDDTPMSKGYNFCPTDTNAAKICNPNIVE
                     NYQNYMEYSYCSHHFTKGQVKVMRRALQGNDGLRSNLITPATHAATGIDLTSAPVCIP
                     TPDIKADKIFTCVGETVQFTDQSFNGPVTFREWTFEDATPATSTSANPSVVFNTFGRK
                     KVTLKVGNASGEVTKVFDKFIDVQDSWAAWIGPKSFDLEPATQYQQLRYHNDGNNYSE
                     FVPVNVGYNSNRSLKLTVYNPSVANALPGSEESRYYNRLSGQKDAIVTAGYDLTHTSG
                     VTFSFDYSYATNAIQSADITEQIRVYYSRNCGETWIPLGNTNQSTIKGTTLTSAGYAG
                     GGDFTPTSASDWGHYSQSFNATSLDTRTRFKIEFTASDFSSNLYIDNINVNGTLGLVD
                     DFTSEHELVISPNPVASGNDLNIQYVAGNEPVTFTLRNLQGEVISTVVRNEVNQPVSF
                     GMEISNNIAASYYLLEVSSTSAKTIKKIAVIK"
     gene            complement(6324..>6621)
                     /locus_tag="PKN22_03880"
     CDS             complement(6324..>6621)
                     /locus_tag="PKN22_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41873.1"
                     /translation="YLPVRQMLLSTIWNSPLDYTNYLPDFVLIACEGDFLEALDCLTI
                     LENMPGEIEERHILEAQWHLKEYLENPEPKEERKAQIISEIALFIKDLDRNIEG"
BASE COUNT         2060 a         1232 c         1138 g         2191 t
ORIGIN      
        1 caccactttt caattgcttg atttgagcat cttgctccac gccaccaaac aaccccatca
       61 ctttcacact tgtttttttg cctatagttt gaaaaacttc ggctatttgt tgcgccaatt
      121 cgcgggttgg aaccatcacc aaacaacgca catttccttt tgtatatttt gatttttggg
      181 ctaaaaaatg caaggttgga attgcaaatg cagctgtttt acctgttcca gtttgagcaa
      241 ttgccattac gtcttcacca tccaatatag gttttatggc tttgtactga atgtcggttg
      301 cacgcacaaa tcccatttct tccaattgat ctttgatggc tttattgatg ttgtattctt
      361 tgaatttcat gtgtttttgg attgctgatg gaaaataaaa cgactcatct tttactaata
      421 caaaagtatc aactttttgg cataaattga tttaatcgca aatcattcgg aattttttaa
      481 acagctgaaa tagattttgt atatttgtga catgaaaaag caatttttaa tattgacttt
      541 aactgtggcg tttgtttttc ttgttgacct tgcttttggt caaggatgtt cacagtgtaa
      601 aatggtttca gaacaatctg gtgaggcagg tgaagcggct tttgcgtcca atatcaacat
      661 tggaattttg ttcttgatgg caatgcctta cattattgtt tttgcggtgt ttagaaaaaa
      721 attattcgct tttttcaaaa gctttttctt tactcccaat caatcatcca aataaactcc
      781 tgttttcatg aaatacctca ttgttggatt gggaaatcct ggtagtgaat atgcagaaac
      841 acgccacaac attggtttta aagtgttgga tgcattagca aaggaatcag cggctacgtt
      901 taaatccgat aaattggcta cttgcacaga agtaaaattc aaaggacgca cattggtgtt
      961 ggtaaaacct acaacataca tgaatttatc cggaaaagca gtaaactatt ggatgcaagc
     1021 acataaaatt cccttagaaa acgtgctggt aatcaccgat gatattgcct tgccgtttgg
     1081 taaattgcgt atgaaaggca aaggaagtga tggcggccac aatggtttaa aagatattca
     1141 agcgcaactt aaaacacaag aatatgcccg tttgcgcttt ggggttggaa gtgattttaa
     1201 caaaggtcga caagtagatt atgttcttgg tgaatggtcg gcagaagaaa aacaaaccat
     1261 cgacgaacgc attgcccttg ccattgaatt tatcaaagga tttgcaacca ttggactgaa
     1321 ccaaacaatg tccaacctca acaataaata agcttatttt ttctttgtag cttttggctt
     1381 tgctttggca ctagcttttg ctttcgttcc cgttgatttt tgatttttgc tgatttcaac
     1441 acaatcttca taggtcaatt tttcgggctc tgttcctttt ggcaatttga aattgtcttt
     1501 tccaatcttc aaataagctc caaaacgacc gttcaataat tgcattgttt cgtcttctgc
     1561 aaacgttttg ataattttat tggcatctgc ttcttgtttt gcaaccaaaa tttcaacagc
     1621 tctttcaaac gttatggtca ttgggtcttc tccttttgga atagatgcaa acacttttcc
     1681 atgctgaatg tatgggccaa aacgtcccac gtttgctttc acaactaagc catcgtgctc
     1741 acctaaagaa cgcggaagtt taaacaaatc aatcgcctct tctaatgtaa ttgaatggat
     1801 agattgctcg cgacgaagtg aagcaaattg cggtttttca ccatcttctt gttcgtcacc
     1861 aatttgaatc ataggtccaa aacgaccaat gcgtgcaatg attttcttac cacttgttgg
     1921 atgaacacct aattctcttt cacctgttgc acgctcagaa tgctccaacg tatcggtaac
     1981 atgagaatga aaaggattat aaaactcatg aatcatcttg gtccactcct tcaatccttg
     2041 tgcaatttta tcaaattgtt cttccacatc cgcagtaaaa tgataatcca tgatgtttgg
     2101 aaaatgttct tccaagaaat cagtaaccac caaaccaatg tcagttggag ataatttatt
     2161 tttttcctta ccagttgttt ctgtttttac aaccgattga atactaccat ttttaagctc
     2221 gtaaaccgta tattttcttt cttcgccttc caattcgcgt ttttcaacat atccacgttt
     2281 ttggatggta gaaattgttg gtgcgtaggt agaaggacgg ccaattccta attcttctaa
     2341 tttttttacc aaagaagctt ccacatagcg agatggtgga cgagtaaaac gttgcgtagc
     2401 gataatacta tctgttttca acaattcacc aacttctaca cttggcaaca agccttctgt
     2461 gttttcatcc tcttcatcgt ccaaagaaga ttcaatatag acacgtaaaa aaccatcaaa
     2521 cttaataacc tccccttttg cagtaaaatg ttgtgcaatt ttatctgttt caatggtaat
     2581 ggttgttcgt tccaattcag catgactcat ttgcgatgca atggcacgtt tccaaattaa
     2641 atcatacaag cggtcttcgt cttttcctaa atcaatggaa tgtgcagcaa aattggttgg
     2701 gcgaatagct tcgtgcgctt cttgtgcacc tgatgaagca gttttgtatt tagttggatt
     2761 cgaataactt gctccgtaac ttttcaccac ttcattttta gctccttcaa ttgcagtgtc
     2821 ggataaatta accgaatccg ttctcatata cgtaatgaac cctgattcat atagtttttg
     2881 agcaacagtc attgtgcgcg aaaccgagaa acctaatttc aaactggctt cttgttgcaa
     2941 ggtagatgta gtaaatggtg cagccggttt tttagtagcc ggttttttct gcacatcagc
     3001 cactttaaat tgagcgtctt tgcagttctc caacaacgtt tctgcttctt tttctgattt
     3061 taattgtgta gaaagttccg atttaatttt accattcttt gtggtgaaat ttccattcac
     3121 acggtagaat gaattcacag aaaatttttc aatctcacgt tctttttcta caattaattt
     3181 aacagctacc gattgcacgc gaccagcaga aagacttggg cgcacttttt tccataatac
     3241 tggtgaaagt tcaaacccca caattctatc caacaccctg cgagcttgct gagcgtttac
     3301 caattcttga ttaatttgac gtggattttc aatagctttt aatatagcag gttttgtgat
     3361 ttcgttaaaa gtaatgcgtt ttgtttcttt ttttgaaaga ttcaacgctt cgtataaatg
     3421 ccaagaaata gcttctccct cacggtcctc atcggttgct agccatacaa catctacttc
     3481 ttttaccaat ttttttaaat ccgaaaccac cgttttttta tcagatgata cttcgtatga
     3541 tggagtaaag ttgttttcaa tatcgatggc aagccctttt tttgctaaat ctcttacatg
     3601 tccgaaactg gattttacaa taaaatcttt tcctaaataa ccttcaattg tttttgcttt
     3661 tgcaggtgac tcaacgataa ccagattttt tgccatagta agaaatttaa actcaattgg
     3721 aatgcaaaac tacgtataaa tcttttgaaa attaaaaaat atatttcaat tcaataaaaa
     3781 ataacaatgc aaacaactgt ttatctgtaa gataaatact attaataaat agtgtaaaaa
     3841 tcaaattgat tataggaaat ttcaattaaa gaagaatttt caaccgttta atcaataaaa
     3901 ggtgttattc aagaaatagc catgaaacat aaatataaat ctcttaactt tacctcattc
     3961 aactttaatt ttattactac ataaattaaa aaagaatagg cagtttgata aagctatttt
     4021 atgaaaagat gaattaaatc acttgatgta taaaaaagtt ttataatttt aaccaaaatt
     4081 tttgttaaat atgaaaagaa tattacatgt aattggagtg gttttggcag gaacttttgt
     4141 tgcaaatgcg cagcaattct cttgtgctac agatcattac taccatcaat tagtacaaca
     4201 agatccttct atagttaagg atttagaaaa cttgattgca aatggagtgg ctgaaaaagc
     4261 agatgaagat tctacaacaa tattcactat tccagttgtg tttcacgttt tgcaccaaaa
     4321 tggagcagaa aacatacctg atgccaacat cattgatcaa atcaatatct tgaacagaga
     4381 ttatatgact ttgaatgctg atacaagttt agtaatccca caatttcaag gtaaaattgg
     4441 aaaagcaaaa attcaattca aattagctgc ttatgatcct tggggaaatt gtacaaatgg
     4501 tatcgaccgt atttatacac atgaaacatc caatggtgga gcatactcaa aagtaaacca
     4561 atgggaccgt tcaaaatact taaatatatg ggttgtaaag gcaatgccag aagttagtgg
     4621 tgctgcaggt tatgctttgt atccaacagc agtaaacggt tatgcttatt ggatggatgg
     4681 aattatgatt ttatataact atgttggttc attatctcca agttctgtgt ataactcaag
     4741 agctataact cacgaggtag gtcactggtt agcattacca catgtttggg gtgataacaa
     4801 cgatcctgga gttgcatgtg gtgacgatgg tatagatgac acacctatgt caaaaggata
     4861 taacttctgt ccaacagaca caaatgctgc aaaaatttgt aatccaaaca ttgtagaaaa
     4921 ctaccaaaac tatatggagt attcttactg ttcgcaccat tttacaaaag gacaagtgaa
     4981 agtaatgcgc cgtgcattgc aaggaaatga tggtttgaga agtaatttga ttactcctgc
     5041 aactcacgca gcaacaggaa tcgacttaac aagtgctcca gtttgtatcc caacaccaga
     5101 cattaaagct gataaaattt ttacgtgtgt tggtgaaaca gtacaattta ctgatcaatc
     5161 ttttaacgga cctgtgactt tccgtgagtg gacttttgaa gatgctactc cagcaactag
     5221 cacaagcgcc aatccatcag ttgttttcaa tactttcggt cgtaaaaaag taacgttgaa
     5281 agtaggaaat gcatcaggtg aagtaactaa agtatttgac aagtttattg atgttcaaga
     5341 ttcatgggca gcatggattg gtcctaaatc atttgactta gaaccagcaa ctcaatacca
     5401 acaattaaga taccacaacg atggtaataa ctatagcgag tttgtacctg taaatgttgg
     5461 ttataacagt aaccgttcat taaaattaac tgtttacaac ccttctgttg ctaatgcatt
     5521 accaggttca gaagaatctc gttactacaa ccgcttgagt ggccaaaaag atgctatcgt
     5581 taccgctggc tatgatttaa cacacacttc tggggttacc ttctcttttg attattcata
     5641 cgcaacaaat gctatccaaa gcgctgatat tacagagcaa attcgtgtgt attattcaag
     5701 aaactgtggg gaaacatgga tccctcttgg aaacaccaac caaagtacaa ttaaaggaac
     5761 aactttaact tctgctggtt atgcaggtgg tggtgatttt actccaactt ctgcaagtga
     5821 ctggggtcat tattcacaaa gtttcaatgc aacttctctt gatacaagaa cacgtttcaa
     5881 aattgagttt acagcatctg acttctcaag taacttatac atcgacaata tcaatgtaaa
     5941 tggtacttta ggattggttg atgatttcac aagcgaacac gaattggtaa tttctccaaa
     6001 ccctgtagct tctggaaatg atttgaatat tcaatatgtt gctggtaacg agcctgtaac
     6061 atttacttta agaaacttgc aaggtgaagt aatttctact gtagttagaa acgaagtaaa
     6121 ccaacctgtt tctttcggaa tggaaatttc aaacaatatc gctgcatctt actatttgtt
     6181 agaagtaagt tcaacttctg ctaaaacaat taagaaaatt gcagttatca aataacaaag
     6241 atttttaaat gtaaaaagag ctgtttcatt acgaaacagc tctttttttg tggtattttt
     6301 tctaaaccat taggcgcgct taactatcct tcaatatttc tatccaagtc tttaataaat
     6361 aaggcgatct ctgaaataat ttgagcttta cgttcttctt ttggttcagg attttcaaga
     6421 tactctttca aatgccattg tgcttctaaa atatgacgtt cttcaatttc gccgggcatg
     6481 ttttctaaaa tcgttaaaca atccaacgcc tctaagaaat caccttcaca agcaattaaa
     6541 acaaaatctg gtaaataatt cgtgtaatcc aacggtgaat tccaaatggt agaaagcaac
     6601 atctgacgca caggtaaata g
//
LOCUS       DAOQVQ010000083         6585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12858_length_6585_cov_3.868760, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000083 DAOQVQ010000000
VERSION     DAOQVQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12858_length_6585_cov_3.868760"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PKN22_03885"
     CDS             <1..1389
                     /locus_tag="PKN22_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS41874.1"
                     /translation="NTIFQPFIAKTTNGGVNWYGNDTLINPSGTPFFNFGDPAPMIDK
                     DGRFLIFASDLPGGFGGLDLWSTTYDKKSDSWSAPKNLGPEINTNGNDVFPTFAKNGD
                     LFYATDGKPGIGGLDIFRITKQGEEYAWSNPVNMGSPINSANNDYGLVEHTDRTGYFT
                     SERKSEFGESKPDIYKYEIPPYVFDLKVIVNDMNDKTSKIADAKVTVTSSTGDKWEGY
                     TNKQGVVFWDKKPNGDRYVNEAASYTINVSKDGYHDAAGSKLTTVGLKYDQNFVIDLS
                     LLPKKPIRLPEVRYALAKWDLMVDSTINSKDSLNFVYDLLNEYPGMVLELSSHTDSRG
                     GTNPNQVLSENRAHKCYEYLVKEKGVDPRRIVPVGKGEADPRFVYRNGDNFSVTLPKD
                     AEGNAQAGWTEVRLTEALINSYKTKDKALFEMLHQWNRRTEGKVITLDFNPATAPAAP
                     ASYLEFKTVPKK"
     gene            complement(1742..3409)
                     /locus_tag="PKN22_03890"
     CDS             complement(1742..3409)
                     /locus_tag="PKN22_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNS41875.1"
                     /translation="MQKIIKYILRLIFIPTELALLFLFGMLLYIMLHATVDEPKATPK
                     KIGKREKIDENYYVLGNNYLKKNDFGIWEMYIEGDPYERGLIYGELAKELNHHQEKVF
                     VDQINEFVPKGFFQSFLNIMVGFFSNTIPDYIPLENQLEIYGISKTFSDEFDYVAPKY
                     TRILSYHAAHDMGHALNDYSVVGCTSFALKGEKTANSNLILGRNFDFYVGDEFAKEKI
                     LLFVRPSKGYKFASYSWAGFTGVVSGMNDQGLTVTINASKSDLPTATKMPISLLAREI
                     LQYAKNIKEAVAIAKKRETFVSETLMIGSANDGKTVLIEKSPKKVGVYEMQGDDLICS
                     NHYQSTTFRNDPVNIENIENSDSKYRYDRVRELIDKHTNKFTPFDVASILRNQKAANG
                     DTLGMGNPRAINQLLAHHSVVMLPTERLFYISTNDFQLGTFMGYDLTKTFKIKDGFIA
                     DTIAADPFLYSNNYQEFLAFKKTKQAINQFLMFDKPLTLSESDIKTFIASNGESYVTY
                     EMLGNYFRKKGDKTNAIFNFKKALSKRTDSKNTSKALEELIKKEAGK"
     gene            complement(3479..3952)
                     /locus_tag="PKN22_03895"
     CDS             complement(3479..3952)
                     /locus_tag="PKN22_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS41876.1"
                     /translation="MEIEFLSEWGQLDAIDQQILLLLKSNAKLGTKEIAAQIGLTTTP
                     TYERIRRLEKRGIIKSYTAIIDKKKQGLTLSVLCNVQLKSHAAEYLEEFEQAIIQLDE
                     VTDCFHIAGNFDYLLKIYIKDMDSYAVFVKEKLAVIPHIATVQSSFVMRTLKEEY"
     gene            4076..5332
                     /locus_tag="PKN22_03900"
     CDS             4076..5332
                     /locus_tag="PKN22_03900"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HNS41877.1"
                     /translation="MDYSKMRPESLMMTYGYNPKLSEGALKCPIFQTSTFVFNSAMEG
                     KRFFELAYGLSEAKQGEQLGLIYSRLNNPDLEILEDRLCLWDKAEACAIFESGMSAIS
                     TVMLEFLSPGDLLLYSRPVYGGTDHFINHVLKRMNIDSVGFAPDSTKEEIIELIKQTG
                     KADKLGLIHIETPANPTNALIDIEMCAAIAKEFSTTEKQVVLSVDNTYMGPMWQHPLQ
                     HGADIVVYSATKYIGGHSDVIAGAVLGSNALMARIKGLRTFLGNMAAPFTGWMLMRSM
                     ETLKVRTEQQARNAKEVAQFLQQHPKVSKVYYLGFIPEEGNDDQKAIYQKQYTSAGAM
                     ISFDIVGGEAAAFKFLDSLQLIKLAVSLGGTESLAEHPYTMTHADVPNDIKNNMSITE
                     NLIRISVGVEFYEDIIADIKQALDCL"
     gene            complement(5392..6162)
                     /locus_tag="PKN22_03905"
     CDS             complement(5392..6162)
                     /locus_tag="PKN22_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNS41878.1"
                     /translation="MRITDRRYLDVYNQAIGHEISNLIRQFDFSEASKNFDYLTKSSA
                     VYSSNIEGNSIDLNSFMNYELNKDKFKSGKEIEEIEDLIGAYEFAQKNKLTEQNLLTC
                     HQLFSKTFLIKSKRGKYRIEQVGVFGKSGLAYMVVEPEFVTQEMSNLFEDIETLISSE
                     LTVEEVFYFAAFIHLRFAHIHPFRDGNGRAARLLEKWFLVEKLGNHYWKIASEEYYKK
                     NQTLYYETINLGVNFYELNYEHCLPFLTMLPNALRTTL"
     gene            complement(6242..>6585)
                     /locus_tag="PKN22_03910"
     CDS             complement(6242..>6585)
                     /locus_tag="PKN22_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS41879.1"
                     /translation="PLPNLGVSNTIRGAVANWSKTLASELGQYGITVNNVLPGATNTG
                     RLQSIAENKAQKTGESTTEIFDEMAKESPMQRIAEPIEVANAIVFLASPEASYINGIN
                     VPVDGGRTKSL"
BASE COUNT         2114 a         1128 c         1210 g         2133 t
ORIGIN      
        1 aatactattt ttcagccttt cattgcaaaa actacaaacg gcggagtgaa ctggtatgga
       61 aacgatacac taattaatcc ttccggaact ccttttttta acttcggtga tccggctcct
      121 atgattgata aagacggaag atttttaatt tttgcttctg atttaccagg tggatttggt
      181 ggtttggatt tgtggagcac aacttatgat aaaaaatcag attcttggtc ggcacctaaa
      241 aacttaggac cagaaatcaa taccaacggt aatgatgttt tcccaacatt tgctaagaat
      301 ggagatttat tttatgcaac tgacggtaaa ccaggaattg ggggattgga tattttccgc
      361 ataacaaaac aaggtgaaga atatgcttgg tcaaacccag ttaatatggg gtcgccaatc
      421 aactctgcaa acaacgatta tgggttagtg gagcatacag atagaactgg atattttact
      481 tctgaaagaa aaagtgaatt tggtgaaagc aaaccagata tttataaata tgagattcca
      541 ccgtatgttt tcgacttgaa agtgattgtt aacgacatga acgacaaaac atctaaaata
      601 gcagatgcga aagttactgt tacttcatca acaggtgaca aatgggaagg atatacaaat
      661 aaacaaggtg ttgtattctg ggacaaaaaa cctaacggag atcgttatgt aaatgaagct
      721 gcttcttata ctatcaacgt atctaaagat ggttaccacg atgcagcagg ttctaaatta
      781 acaactgttg ggttgaaata cgaccaaaac tttgtgattg acttatcatt gttgcctaaa
      841 aaacctattc gtttgccaga ggtgcgttac gcattggcaa aatgggattt gatggttgat
      901 tctacaatta attcaaaaga ctcattgaac tttgtttacg acttgttgaa cgaataccca
      961 ggaatggtat tggagttaag ctctcacact gactcacgtg gtggtacaaa tcctaaccaa
     1021 gtgctttctg aaaatcgtgc acacaaatgt tacgaatatt tagtgaaaga aaaaggtgtt
     1081 gacccacgta gaattgttcc tgttggaaaa ggtgaagctg acccacgttt tgtttataga
     1141 aatggagata atttctcagt tactttacca aaagatgctg aaggaaatgc gcaagcaggt
     1201 tggacagaag ttcgtttgac tgaagcttta attaattctt ataaaacaaa agacaaagca
     1261 ttgtttgaaa tgttgcacca atggaatcgt agaacagaag gtaaagttat tacacttgac
     1321 ttcaatccag caacagctcc agcagcacca gcttcttact tagaatttaa aacagttcct
     1381 aagaagtaat ttatagatag aattcattca aaggtcgttc agtaatgggc gacctttttt
     1441 gttatcatga aagtgtatat cttagaggta aatacaggtg taaaagtcaa atacagtgag
     1501 taaacactta cttgcttttt tagtgctacg agcctcagcg tattaatatg ttggtggcat
     1561 aaagtgttat attttataac tcaattaaaa gtcagtaaaa catggtaatg actaaacatc
     1621 ctatttgtat gctattataa tttgttgtaa ttattttaga caaaattaaa tacaggcaat
     1681 tgcatggtta tggcacaaga caaatgatat caatcaataa acaaattaaa atgagtgcta
     1741 attactttcc agcttccttt tttattaatt cttccaaagc tttgctggtg tttttagaat
     1801 cggtgcgttt agaaagtgct tttttgaaat tgaaaatagc gttggtttta tcgccttttt
     1861 tcctaaaata gttgcccaac atttcgtagg taacatacga ttctccgttg cttgcaataa
     1921 atgttttaat gtccgattct gaaagagtca acggtttatc aaacatcaaa aattgattga
     1981 ttgcttgttt tgtttttttg aacgctaaaa attcttggta gttgttagaa tataaaaacg
     2041 gatcggcagc aatggtgtcg gctataaacc catcttttat tttaaatgtt tttgttaaat
     2101 cataacccat aaaagtaccc aattgaaaat cgttggtaga gatgtaaaac aaacgttctg
     2161 ttggcaacat caccacgctg tgatgcgcta ataactgatt gatagctctc ggatttccca
     2221 tacctaatgt gtcgccatta gctgctttct gattgcgcaa gatggacgcg acatcaaaag
     2281 gagtaaactt attggtgtgt ttgtcaatca attctcttac tctatcatag cggtatttgc
     2341 tatcactgtt ttcaatgttt tctatgttca cagggtcgtt tctaaatgta gtagactggt
     2401 aatgattgga gcaaattaaa tcatcgcctt gcatttcgta aacccctact tttttaggcg
     2461 atttttcaat taaaacagtt tttccatcgt tagcagaacc aatcatcaac gtttctgaaa
     2521 caaatgtttc acgtttttta gcaatggcaa cagcttcttt tatgtttttg gcatattgca
     2581 aaatttcacg agccaacaag gaaataggca ttttggttgc tgtaggtaaa tccgattttg
     2641 aagcgttgat ggtaacggtt agtccttggt cgttcattcc agaaactact cctgtaaatc
     2701 cagcccatga ataggaagca aatttatatc cttttgatgg tcgaacaaac aaaagaattt
     2761 tttctttagc aaattcatcg cccacataaa aatcaaagtt tcttcctaaa attaagttag
     2821 aatttgcggt tttctcacct ttcaacgcaa aagaggtaca accaactaca ctgtaatcat
     2881 tgagcgcatg ccccatatcg tgtgcggcat ggtaacttaa aatgcgggtg tattttggtg
     2941 caacataatc aaactcatct gaaaaagttt tggaaatacc ataaatttcc aattgatttt
     3001 cgagcggaat gtaatctgga atagtgttgg agaaaaaacc aaccataatg ttcaaaaaac
     3061 tttggaaaaa tcccttaggt acaaactcat tgatttgatc cacaaacact ttttcttggt
     3121 gatggttcaa ttcttttgct agttcaccat atatcaagcc tctttcatac ggatcgcctt
     3181 caatgtacat ttcccatata ccaaaatcat tttttttcag atagttgttg cccaacacat
     3241 agtagttctc atcaattttc tcacgttttc caattttttt gggagttgct tttggttcgt
     3301 caacagttgc atggagcata atgtataaga gcattccaaa taaaaataac agtgctaatt
     3361 cagttggaat aaaaatgaga cgaagtatgt atttgatgat tttttgcaca acttataaaa
     3421 tagactacaa aaatacgata tttctgaatg ttaatggaat aacgattgaa aataaacgtt
     3481 aatattcttc ttttaacgta cgcataacaa atgaactttg aaccgttgca atgtgaggaa
     3541 taacggctaa tttttctttc acaaacaccg cataactgtc catgtctttg atgtatattt
     3601 ttagcaagta gtcaaaatta cctgcaatgt gaaaacaatc ggttacttca tcgagctgaa
     3661 taattgcttg ttcaaattct tccagatatt ctgctgcatg cgattttaat tgcacattgc
     3721 acaatacgct caacgttaac ccttgttttt tcttatcaat gatggctgtg taggatttga
     3781 tgattcctct tttttctaac ctacggatgc gctcataggt aggggttgtt gttaacccaa
     3841 tttgggcggc aatttctttt gttcctagct ttgcattcga ttttaatagc agtaaaattt
     3901 gctgatcaat tgcatccaat tgtccccact ccgataaaaa ttctatttcc attataaatt
     3961 tgttatgtat atagttgaaa aaactattaa aaatcaaaaa tacagtaata aagactatta
     4021 tgaaaataat aatagaaaaa aaacctatat ttactaaaga aaatagaaca ataatatgga
     4081 ttactcaaaa atgcgtccgg aaagtttgat gatgacttac ggatacaacc ctaaattatc
     4141 agaaggagct ttaaaatgcc ctatttttca gacatcaacg tttgttttca attctgcgat
     4201 ggaaggaaaa cgcttttttg aattggctta tggtttaagt gaggcaaaac aaggagagca
     4261 attaggtttg atttacagtc gtttaaataa tcctgattta gaaattttag aagaccgttt
     4321 gtgtttatgg gacaaagcgg aagcatgcgc tatttttgaa agtggaatgt cggcaatttc
     4381 tactgtaatg ttggaatttt tgtcgccagg agatttgttg ttgtattcac gcccagttta
     4441 tggtgggaca gaccatttca tcaatcacgt gttgaaacgt atgaatattg attcggttgg
     4501 ttttgcacca gatagcacca aagaagaaat cattgaatta atcaaacaaa ctggtaaagc
     4561 tgataaatta ggattgattc acattgaaac acctgccaat ccaaccaatg cgttgattga
     4621 tattgaaatg tgtgcagcca ttgctaaaga attttcgacg acagaaaagc aggttgtttt
     4681 atcggtagat aatacctaca tgggaccaat gtggcaacat ccattgcaac acggagcgga
     4741 tattgtggtg tattctgcaa caaaatacat tgggggtcac tcggatgtga ttgctggcgc
     4801 tgttcttggt tcaaacgctt tgatggctag aatcaaagga ttgcgtacgt ttttgggtaa
     4861 tatggcagct ccatttacag gttggatgtt gatgcgaagc atggaaacac taaaagtacg
     4921 tacagagcaa caagcacgca atgcaaaaga agtagctcaa ttcctccaac agcatccaaa
     4981 agtgagtaag gtttactatt taggctttat tccagaagaa gggaatgacg atcaaaaagc
     5041 aatttaccaa aaacaatata catctgcagg agccatgatt tcttttgaca tcgttggtgg
     5101 agaagctgca gcattcaaat ttttggatag ccttcagtta attaaattgg cggtaagctt
     5161 gggtggaact gaatcattgg cggagcatcc ttacacgatg acacatgccg atgtacccaa
     5221 cgacattaaa aacaacatgt cgattacaga aaacttaatt cgcatctctg ttggggtgga
     5281 attttacgaa gatatcattg cggatatcaa acaggccttg gattgtttat aattgaaaaa
     5341 taaagttcaa aagcagtgag ggattgtcta ttgggcaatc ccttctgttt tttataatgt
     5401 tgttcttaaa gcattaggaa gcattgttaa aaacggcagg caatgctcgt aatttaattc
     5461 gtaaaagtta actcccaaat tgattgtttc ataatagagc gtctgatttt ttttataata
     5521 ttcttcagaa gctattttcc agtaatgatt accaagcttt tcgactaaaa accatttttc
     5581 tagtaaacga gcagctcgac cattgccatc acgaaacgga tggatgtgcg caaatcttaa
     5641 atggatgaat gctgcaaagt aaaaaacttc ttcaacagtt aattcggatg aaattagcgt
     5701 ttcgatatct tcaaataaat ttgacatttc ttgtgtaaca aattctggtt caaccaccat
     5761 ataagctaag ccagatttgc caaaaacacc tacttgttct attctatatt tccctcgttt
     5821 actttttatt agaaatgttt tggaaaaaag ttggtgacag gtcaataaat tctgctctgt
     5881 taatttattt ttctgtgcaa attcataagc accaataagg tcttcaattt cttcaatttc
     5941 tttacctgat ttgaatttat ctttattcaa ctcatagttc ataaacgaat tcaaatcgat
     6001 actatttcct tcaatgttgg aagaataaac tgctgaagat ttggtcaaat aatcaaagtt
     6061 tttagaagcc tcagaaaaat caaattgacg aatcaaattg gatatttcat gaccaatagc
     6121 ttgattgtaa acatctaaat atcttcgatc tgtaattctc attttaacca attttcaatc
     6181 aaaaatacac aatatttttt ggtataggca ccgttgccta cagctttaaa ttagctaaca
     6241 attacaaaga ttttgttctt ccaccatcaa cagggacatt aatcccattg atataggaag
     6301 cctctggcga tgctaaaaac acgattgcat tggcaacttc tatgggttct gcaatacgtt
     6361 gcatgggtga ttctttggcc atttcatcaa aaatttcggt ggtagattca cctgtttttt
     6421 gtgccttatt ttcggcaatg ctttgcaaac gtccggtatt ggttgcgcca ggcaatacat
     6481 tgtttaccgt tatgccatat tgccccaact cacttgccaa cgttttgctc caattagcaa
     6541 ccgctccacg aatggtgttg gaaacgccta aattgggcaa aggtt
//
LOCUS       DAOQVQ010000084         6571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12896_length_6571_cov_3.615101, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000084 DAOQVQ010000000
VERSION     DAOQVQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12896_length_6571_cov_3.615101"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..573
                     /locus_tag="PKN22_03915"
     CDS             112..573
                     /locus_tag="PKN22_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41880.1"
                     /translation="MLKILFTSFILLILISCKTSTNSLELSKGEKIFLSRLSEKKSGL
                     KYGKHLIFILNESSCQPCEDKVKAFVASNEWNGLDKLFIVTEDRNPLNEEIDNYTVFK
                     YKDLASYGLVRPNGTILIIKNNECVYLESIDINHLDEIKSNMLSILNQNKK"
     gene            complement(574..1164)
                     /locus_tag="PKN22_03920"
     CDS             complement(574..1164)
                     /locus_tag="PKN22_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41881.1"
                     /translation="MESFFVNLGLSYTWSKALPYILLVVLGIVLGLFLFKKLQSKVGK
                     LLALLLMVIPFTVYFIFYPIYEGDFTNEARIVQHSDKTQELQGKKLIVISLPGCPFCK
                     QSVLFFKEIKKKHPHIKVEYVVTSDDDRNLAYYDEVIQGAFPLRLANNPEAMSKLAQG
                     SFPTYVLVDNNQPLKIWSNNTFGVMALDDVVDSFKK"
     gene            complement(1171..2397)
                     /locus_tag="PKN22_03925"
     CDS             complement(1171..2397)
                     /locus_tag="PKN22_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNS41882.1"
                     /translation="MRNNKRKIFNDPVYGFISIPNEFIFDLIEHPYMQRLRRIRQLGL
                     THLVYPGALHTRFHHVLGAMHLMNLAIQVIRRKGHEITNDEEEAVLAAILLHDIGHGP
                     FSHALEHTIVTGVSHEAISDYFMKRLEQEFGAKLHMAREIFSNSYKKPFLYQLVSSQL
                     DMDRMDYLNRDSFYTGVSEGIIGSDRIIEMLNVHNGELVLEEKGIYSVEKFITARRLM
                     YWQVYLHKTVVSAEHLLINTLKRAKYLSNQGIELFASPALSFFLKNNITHHDFENNPT
                     VLDTFAQLDDYDIMGAIKVWQTADDKLLADLSKRIIDRKLFKVKIAKEPFSQEEINDK
                     KAKVAQQMNIGINETDYFVFATNLVNNAYNQEKQNINLIMKNGKVVDITEASDNLNIS
                     ALSQPVEKYIFCYPEI"
     gene            complement(2452..2691)
                     /locus_tag="PKN22_03930"
     CDS             complement(2452..2691)
                     /locus_tag="PKN22_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41883.1"
                     /translation="MLRKVKLMWDFRGSDSEERARRHEHHLEEYLEGKKLGYRMTGMK
                     TESEDFSYSYMIVEEDEMISFRDALRPTRGGWIEL"
     regulatory      2907..3001
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3063..5288
                     /locus_tag="PKN22_03935"
     CDS             3063..5288
                     /locus_tag="PKN22_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS41884.1"
                     /translation="MKCIHLSFKTALIAGVFTLCANETQAQVDSSTIKQEKELIPIEI
                     RAIRVNDKNPYAVSNVDSLMIRQSNGVQDIPYLLDQTPSVTVSSDAGAGVGYSSMRIR
                     GTDASRINFTMNGVPINDAESQAAIFVDFPDILGSANSIQIQRGVGSSTNGSGAFGAS
                     VNMSNITQGDKPYASIHSAVGSFNTFKNSLKIGSGKLKGGFQFDMRLSHIKSDGYIQR
                     AFSNLKSLQFIAGWTSKNEKSSLRFNLLMGREKTGQAWNGVAQDSLKTNHRFNSLGLM
                     QDGTYYNDQTDNYGQDYYQLIYNQIINDYWSTNITLFMTRGKGYYNEYKLGESFSDYN
                     YQPFVTPSNDTLFSTNLTRQLWLDNYYYGTSFAVHYQKKNTQLDLGGNISLYDGKHYG
                     FIKWADYGFPIDHNWYNLTSFKNDYSVYAKWQQQFGKGFYLFADLQYRHVDYKMNGFR
                     KSISTRPSVQYDFLNPKIGLSYIHAHANAAKSKIYGSFAMANKEPNRDDFETASYEMP
                     KHETLLDAELGYEYMAGKWGIGLNLYYMYYFNQLIQTGKINDVGAYARQNVPESFRTG
                     AELQVRYQPVRMLTLNANATFSMNKITQFTEYIDDYDNGGQLEVVHKNSDIAFSPSIV
                     AYANATVEPLINILSNQHFFIDINGKFISRQYLDNTSNKLRSINPYALANARLRYTIE
                     TKKFIKEVGVSLTLNNILNKKYESSGYTFSYLYGGETTTENFYFPQAGFNFIFGVQLN
                     F"
     gene            5294..6415
                     /locus_tag="PKN22_03940"
     CDS             5294..6415
                     /locus_tag="PKN22_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41885.1"
                     /translation="MEQVMNSVLTHQFLTAEQLQLIRQESSNIEKNGQLSAPILDVIY
                     ANNWLKIMVPKSCGGLEWSLPQIVRLFETLAHADGSVGWNVNLGAGANLFSGYFSDEV
                     AQSIFSNSKVWCAGSGASTGTAQRVENGYLINGYWKYASGSAHATHFTANCKLLDEDG
                     NLITEDGKAVFRSFIFPKEDVTVHNTWNVMGLKAIASNDFEIKDLVVSEQAVFTLLKP
                     SEFASAPIFKVPFDTLAFSNMASMVVGMTAHFLELFATEILPKKPLYCDLKMEERKGV
                     SAAYEKYNAQFSALRLAFYHLLETCWSTYENGVQPSTDDLNTLRLKAKETASLAREIV
                     ANLVPLCGMNIIFEDNEISRVYRDLTVGCQHYLLAPEVE"
BASE COUNT         2108 a         1174 c         1205 g         2084 t
ORIGIN      
        1 ttatacccaa ctgaaaatgg agttttaatt tcgataccag aacttataaa agtttcaaaa
       61 gataaaattg aatttaaatt tttaaaagtt agtcatttta aaaagtagaa tatgttaaaa
      121 atattattta catcatttat attattaatt cttatctcct gtaaaacgtc aactaactca
      181 ctcgaactat caaagggtga aaaaattttt ctaagtcgat tatcagaaaa aaaaagtggg
      241 ctgaaatatg gaaaacactt aatatttatt cttaacgaat catcttgtca accttgtgaa
      301 gataaagtta aagcatttgt tgcctctaat gaatggaatg gtttagacaa attatttatt
      361 gttacggaag atagaaatcc cttaaatgaa gaaattgata attatacagt cttcaaatat
      421 aaagatttag ctagttatgg attagttcga cctaatggaa caattttaat aataaagaat
      481 aatgaatgtg tttatcttga atcaattgat attaatcatt tagatgaaat taaaagtaat
      541 atgcttagta ttttaaatca gaataaaaag taattatttc ttaaaactat ccaccacatc
      601 gtccaatgcc attacaccaa aggtattgtt gctccatatt ttcaaaggtt ggttgttatc
      661 aaccagtaca taagttggaa aactaccttg tgccaattta ctcatagcct ctggattgtt
      721 ggctaatcgc aaaggaaatg ctccttgtat aacttcgtcg taatacgcta aatttctatc
      781 atcgtcggag gtaacaacat attctacctt tatatgagga tgttttttct tgatttcctt
      841 gaaaaataac accgattgct tgcaaaacgg acaaccaggt aatgaaatga caatcaattt
      901 ctttccttgc aattcttgtg ttttatcact gtgttgtact atgcgcgctt cgtttgtaaa
      961 atcgccttcg taaatggggt aaaaaataaa atatactgta aatggaatta ccataagcaa
     1021 caaagccagt aatttaccca cttttgattg taatttttta aacaaaaaca aacccaagac
     1081 aatgcctaaa actactaata aaatgtaagg aagagctttg ctccaagtgt aagaaagtcc
     1141 taaattgaca aaaaatgatt ccatcgtgtg ttaaatttca gggtaacaaa aaatgtattt
     1201 ttcaacgggt tgcgacaatg cagaaatgtt taaattatca ctcgcttcgg tgatgtccac
     1261 aactttaccg tttttcataa tcaaattgat gttttgcttt tcttgattat aggcattgtt
     1321 aaccaaattg gtggcaaaaa caaaataatc ggtttcgttg atgccgatat tcatctgttg
     1381 cgcaactttt gcctttttat cattgatttc ttcttgagaa aacggttctt tcgcaatttt
     1441 tactttaaac aatttacgat cgatgatgcg tttggataaa tcggctaata gtttgtcatc
     1501 agcagtttgc caaactttga ttgctcccat gatgtcgtaa tcatccaatt gtgcaaaggt
     1561 gtctaaaacc gtcggattgt tttcaaaatc gtgatgcgta atgttatttt ttaagaaaaa
     1621 cgaaagagca ggtgatgcaa aaagttcaat tccttggttg gacaaatatt ttgcacgttt
     1681 gagggtgttg attaacaaat gttcggcact tacaacggtt ttgtgcaaat acacttgcca
     1741 atacatcaat cggcgtgcag tgatgaattt ctctaccgaa tagatgcctt tttcttccaa
     1801 taccagctcg ccgttgtgca cgttcaacat ttcaatgatg cggtcactac caataattcc
     1861 ctcactcaca cccgtgtaaa aactatcacg gttgaggtaa tccatacgat ccatatccaa
     1921 ctgactggaa accaattggt ataaaaaagg ttttttgtag gagttggaga atatttcgcg
     1981 tgccatgtgc aatttagcgc caaattcttg ctccaaacgc ttcatgaaat agtcggatat
     2041 tgcttcgtgg ctaactcctg taacaatagt gtgttccaaa gcgtgactaa acggtccgtg
     2101 accaatgtcg tgcaataaaa tagccgccaa aacagcttct tcctcgtcat tggttatttc
     2161 atgccccttt ctacgaatta cctgaatggc caaattcatc aaatgcatgg cgcccaaaac
     2221 atggtgaaaa cgcgtgtgca atgcgccagg ataaaccaaa tgtgttaatc ccaattggcg
     2281 tattctgcgc aaacgctgca tgtagggatg ctcgattaaa tcaaaaataa attcgttggg
     2341 tatggaaatg aatccataaa cagggtcgtt aaatatcttt cgtttgttat ttctcactga
     2401 aaatgattga aaaaacaaat ttaaaaaata tttgattgaa actaataaaa attaaagctc
     2461 tatccagcca cctcttgttg gacgcaaagc atctcggaaa gaaatcatct cgtcttcttc
     2521 aacaatcatg taggaatacg aaaaatcttc cgactctgtt ttcatacccg tcatgcggta
     2581 acccaatttt tttccttcca aatattcctc caaatggtgt tcgtgtcttc gtgcgcgctc
     2641 ctcagaatcc gaacctctaa aatcccacat caattttact tttctgagca tatttttcaa
     2701 ttttatgcaa aaatactatc aaaaataggt aaaaaatatg attgaagtca taagttggta
     2761 atatttgaac gcaagctctc tttaaatttc aattgctcaa gcgattagct gactttcttt
     2821 aaaattcaat agtgaatatt ccaactttca tttgccaaac agaaaattta cactagcttt
     2881 gtggcgaatt aaacgttgaa taagcgtttt ttcacccggg gtgccttact ttcaggctga
     2941 gatcacaccc gttgaacctt gaccaggtaa tgctggttag gaattgtaaa ctcacttctt
     3001 gaggtgattt gcatttagtt acatagcctg gattgtttac taattgttta attatttcaa
     3061 ttatgaaatg tatccattta tcgtttaaaa ctgcattaat tgcaggagtg tttactttgt
     3121 gtgccaatga aacacaggca caggttgatt cttcaactat caaacaggaa aaagagctca
     3181 ttcctataga aattagagcc attcgtgtca acgacaaaaa tccttatgct gtttccaatg
     3241 tagatagctt gatgattcgt caatccaacg gagtgcagga tattccgtat ttgttagacc
     3301 aaactccatc ggttactgtt tcttctgacg ctggagcagg tgtgggctac tcgtccatgc
     3361 gtattcgtgg aacggatgct tcgcgtatta actttacgat gaacggtgtg ccgattaacg
     3421 atgctgagtc gcaagcagca atttttgtgg attttccaga tatcttaggt tcggcaaatt
     3481 ccattcaaat tcaacgtggt gttggttcat ctaccaacgg ttcaggagca tttggagcat
     3541 cggttaatat gtccaacatt acccaaggtg acaagcctta tgcaagcatt catagtgctg
     3601 ttggttcgtt caatacgttt aaaaattcac taaaaattgg ttctggaaaa ctaaaaggtg
     3661 gttttcaatt cgatatgcgt ttgtcgcaca tcaagtcgga tggctacatt caacgtgctt
     3721 tttccaacct aaaatcgttg caatttattg ccggttggac cagtaaaaat gaaaaatcgt
     3781 ccttgcgttt caacctctta atggggcgtg aaaaaactgg acaggcatgg aatggcgttg
     3841 cgcaagattc gcttaaaacc aatcaccgtt ttaacagttt aggattgatg caagatggaa
     3901 cgtactataa cgatcaaact gataattatg ggcaagatta ttaccaattg atttacaacc
     3961 aaataattaa cgactattgg tcgacaaaca ttacgttgtt catgacacgt ggtaaaggat
     4021 attacaacga atacaaattg ggcgaatcgt tctcggatta caattaccag ccctttgtta
     4081 caccgtccaa cgataccttg ttctctacta atttaacccg ccagttgtgg ttggataatt
     4141 attactacgg aacttcgttt gccgtgcatt atcaaaagaa aaatacgcaa ttggatttgg
     4201 gtggaaacat cagtctttat gatggaaaac actatggttt tatcaaatgg gcagattatg
     4261 gttttccaat cgaccataat tggtataacc tcacctcgtt taaaaacgat tattcggtat
     4321 atgctaagtg gcaacaacaa tttggtaaag gcttttatct atttgccgat ttgcaatacc
     4381 gtcacgtaga ttataaaatg aatggtttta gaaaatccat ctctacacgt cctagtgtgc
     4441 aatacgattt cttgaatcca aaaattgggt tgagctacat tcacgcacac gccaatgcgg
     4501 caaaaagtaa aatttatgga tcatttgcaa tggccaataa agaacccaac agagatgatt
     4561 ttgaaacagc cagctacgaa atgcctaaac acgaaacttt gttggatgca gagttgggct
     4621 atgaatacat ggcaggaaaa tggggaattg gtttgaatct ttattacatg tattatttca
     4681 accaattgat tcaaacagga aaaattaacg acgttggagc ttatgcacgc caaaatgttc
     4741 cagaaagttt ccgcactggt gccgaattgc aagttcgcta tcaacctgtt agaatgttaa
     4801 ctttgaatgc taatgcaact ttttcaatga acaaaattac gcagttcacc gaatatattg
     4861 atgattatga caatggcgga cagttggaag tggtgcacaa aaattcagat attgcttttt
     4921 caccgtcaat tgtagcttat gccaatgcaa ctgttgagcc gttgattaac attctttcca
     4981 accaacattt tttcattgat ataaacggaa aattcatcag tcgtcagtat ttggataata
     5041 cttccaacaa gttgagaagc atcaatccgt atgctttggc aaatgcgcgt ttgcgttata
     5101 ccattgaaac caaaaaattc atcaaagaag tgggtgttag tttaacattg aacaatattt
     5161 tgaataaaaa atacgagtca agtggttata cgttcagcta tttgtatgga ggcgaaacaa
     5221 caacagaaaa tttctacttc ccacaagcag gatttaactt catatttggt gttcaactta
     5281 atttctaagc aacatggaac aagtgatgaa cagtgtttta acacaccaat ttttgacagc
     5341 agagcaattg caattaattc gtcaagaaag cagcaatatt gagaaaaacg gacaattatc
     5401 tgcgccaatt ttagatgtga tttatgccaa caattggtta aaaatcatgg tacccaaatc
     5461 gtgtggaggt ttggaatggt cgttacctca aatcgttcgc ctgtttgaaa ccttggctca
     5521 tgccgacgga agtgtgggat ggaacgttaa tttaggagcg ggagctaact tgttttctgg
     5581 ctatttttcg gatgaagtgg cgcagtccat tttttcaaac tccaaagttt ggtgtgcagg
     5641 aagtggcgca tctactggta cggcacaacg cgttgaaaac ggctatttaa tcaatggtta
     5701 ttggaaatat gccagcggtt cggcacatgc tacgcatttt acagccaatt gcaaactgtt
     5761 ggatgaagat ggcaatttaa ttaccgaaga tggtaaagcc gtttttcgct cgtttatttt
     5821 ccctaaagaa gacgttaccg tgcacaacac atggaacgta atgggattaa aagcaattgc
     5881 cagcaatgat tttgaaataa aagatttagt tgtttcagag caagctgttt ttacgttgtt
     5941 aaaaccctct gaatttgctt ctgcaccaat ttttaaagtt ccgtttgaca ctttagcatt
     6001 ttccaacatg gcaagtatgg tagtgggaat gaccgcacat tttttagaat tgtttgcaac
     6061 cgaaatttta cccaaaaaac cgttgtattg cgatttgaaa atggaagaac gaaaaggagt
     6121 aagtgctgct tacgaaaaat acaacgctca attttcagca ttgcgtttag cgttttatca
     6181 cttgctagaa acttgttgga gcacctatga aaatggagtg caaccatcta ccgatgattt
     6241 gaatacattg cgattaaaag caaaagaaac agcctcttta gcaagagaaa ttgtagccaa
     6301 tttagttcca ttgtgcggca tgaacatcat ttttgaagac aatgaaatca gtcgtgttta
     6361 tagagattta acagttgggt gccagcatta tctattggct cctgaagtgg aatagtagga
     6421 attacaatca caacctacag gaacctcaag agacaataaa tgttttcttg aggttttttt
     6481 gttaaatatt tttgtcaaat tagttgaatt attaaaatat tttattatat ttggcattat
     6541 tatttacaca ttatgaatat actacgctac a
//
LOCUS       DAOQVQ010000085         6568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_12902_length_6568_cov_3.884385, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000085 DAOQVQ010000000
VERSION     DAOQVQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12902_length_6568_cov_3.884385"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3301
                     /locus_tag="PKN22_03945"
     CDS             <1..3301
                     /locus_tag="PKN22_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41886.1"
                     /translation="GGGRGGYSYANTQNYGVSPYVGPNNTGWGGNARKENGGYGGHPI
                     KYDAQRIFMGGGGGAGGSDSGQGGNGGNGGGIVFLTVYGNITGSGSIVANGANGQNTN
                     PNNDGVSFSNKRRGNDGGGGAGGGGYIYVKSMNAIPSTLTLQANGGKGGDVRLTIYYI
                     GGGNPEVTGPGAGGAGGGIAFTSGTPTNSVSGGLPGGTFLAGSQNPMSVNFPMNGATS
                     GGNGMTNLPAHIFDILVQNDTICGSQTSTLTATVSGTFPTGSSIGWYATPFGGLPLAT
                     GTTFTTPVLTSTTTYYVGNCPGTFRKPVQVVVGQNPIIAGTAVITNATCTTGGSITGL
                     SASGGTGALTYAWNGTAAAGPTLSNASAGSYTLTVTDANGCSSQSGPHTITGVGGPTI
                     TGTPTITNQTCLVNGTITGLTVNSSVAITSYDWNGTTSVDQNLSATAGSYTLTVIDAN
                     GCSAQSGPYTIGLAPTPTISGTASITNANCNNGGNISGLTASGGVGTLTYDWNGATSV
                     TTDLSNAAAGSYTLTVTDANGCTAQSGPHQINTDANPTITGTAVVTDATCTVQGSITG
                     LSASGTGTLTYDWNGTPSPSIDLTGAGVGSYTLTVTDGNGCTTQSGPYTINVTGGPTI
                     SGTAVISDVTCTTQGSITGLTVSGGTGTLTYSWNSTISPTADLTNATAGTYTLSVADA
                     NGCISQSGPYTINTTPAPTIAGTPVITDATCTTGGSITGITVSGGAQPYTYQWNNNAA
                     SGIDLINVSAGTYTLSITDDNGCVVTSGPHTIGSQGAVTISGTATVTHATCTTGGSIT
                     GLTATGGANPYTFEWNNVTTPSADLTNAQPGSYVLEVTDANGCIATSASYTINAPNTP
                     TIGTTATIVDATCNQGGSISGVVVANGTGPYQYSWNNGQYSTLDITNVPAGSYTLTVT
                     DASGCSVTGTPLTVGQTAAVVAQFTYSPNPVMINENIQFQDESTGNVVAWEWVIEQDT
                     MTIQNPTHTFENDGMYDVSLVVVDANGCVDTAKVTIEVISEMEVPNVITVNGDGINDL
                     FEIKGLLPETKVIILNRWGELIYTSDNYANNWDGKDKSGKTVTEGVYTYLVLAKDGSK
                     KHGFVHVVVKH"
     gene            3336..3914
                     /locus_tag="PKN22_03950"
     CDS             3336..3914
                     /locus_tag="PKN22_03950"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HNS41887.1"
                     /translation="MLDTKKYASTSIGMIYQGYLHEYEEEEKQKVILKFFGEFTINLI
                     RNFADGIENLMAKEGDKRHVVKRMFSILIEGLQNAHIHGGRDEEGEQRSFLIISQNPE
                     QYKITFGNIVEQKDKELVTTYLGNINNHNEEELKQLYIAILKNGYLSHKGGAGLGIVT
                     LRMKSSKLINYRIYELSSSKYFIVMEVCLDRN"
     gene            complement(3911..4645)
                     /locus_tag="PKN22_03955"
     CDS             complement(3911..4645)
                     /locus_tag="PKN22_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS41888.1"
                     /translation="MNAEPLSLNKIKQIICLQQKKCRDKQGLFVVEGEKIVAELIDFA
                     PQQIEYLVATPEFEVTTNTIPQYHCDEATLKKCSSLTTPNKVLAVVKKMEFKNSSPNF
                     MIALDSVQDPGNMGTILRLADWFGVQKVICSKTTVDIYNPKVIQATMGAFLRIQVEYL
                     DLKEFFATTDLPVYGALLEGKNVYAQPLKPHGILLMGNEGNGISDELKPFISKPLTIP
                     KKGQTESLNVSIATSILLSEFFRTSF"
     gene            4890..>6568
                     /locus_tag="PKN22_03960"
     CDS             4890..>6568
                     /locus_tag="PKN22_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41889.1"
                     /translation="MYNAVDSAKVSDKRIRKNEKLKLKNQERRAKEKRINEKRIAKAK
                     RKNREWYTKKVIPLKDTLNPKMFFREWLKYKFGEPPVIFDTAYLSQSISQLDLFLKKK
                     GYYYSEVTGDYTTKGKKAVANYYIKTGKRYYIDSVYVAGPNLSVNQSYLNFIRRGKIE
                     SLEKQPFDSDLLNDYRTDVARLMRDEAFFGFNFSSIDFVADTAKGDYKVTLGIRFMDR
                     MIKSPDYADSFYSVPYKTYLVKSVHFHILDTTRMKGNYDYRMKQLGLVPPKNGEIVTM
                     DTLLFDNIYYNRSEKKRRGFPLNEKVKNPLRMASFYYNGKMFVRPGIIETQNYLENKN
                     YYKEYYLERSYSRLLQLDIFSSVKPKLVELQDSSQVEVHYYLTPAKRQYFAFEPKFTN
                     SNGFLGLSASINYSNRNIFRGAEKLTFSLGGGFESQPPIFDESINGEKIKKAGRSFNT
                     FEIEPGIKLEIPGIFPFRKAAFMAKRQRARTVITTSFNYQNRTDFKRRSFQFGYLWRF
                     YGAETQIFQIGLPTTSSVKYVLFNPSALFEQKLIELNDVFLFNTYSDQFIW"
BASE COUNT         2149 a         1194 c         1331 g         1894 t
ORIGIN      
        1 tggaggaggt agaggaggtt attcgtatgc caatactcaa aattatggtg tgagtcctta
       61 cgttggtccc aataatactg gttggggagg aaatgcgcga aaagaaaacg gaggctatgg
      121 tggtcatcca attaaatacg atgcccagcg catttttatg ggcggtggcg gtggcgccgg
      181 agggtcagat tctggtcaag gaggaaatgg aggaaatggt ggtggtattg ttttcttaac
      241 agtttatggt aacatcacag gctcaggctc tattgttgca aatggtgcaa atggacaaaa
      301 taccaatccc aacaacgatg gtgttagctt cagcaacaag cgtcgtggaa atgatggtgg
      361 cggtggtgct ggcggtggtg gctacattta tgtaaaaagt atgaatgcaa tcccttcaac
      421 ccttactttg caagctaatg gaggtaaagg tggggatgtg cgcttaacca tctattacat
      481 aggtggcgga aatcctgaag taacaggacc cggagctggc ggtgctggtg ggggaattgc
      541 ttttacatct ggaacaccta ccaattcggt tagtggagga ttacctggtg gtacattttt
      601 agcaggttct caaaacccta tgtcggttaa ctttccaatg aacggcgcaa cttctggcgg
      661 aaatggaatg acaaatttac ccgcacacat ttttgatatc ttggtacaaa acgacaccat
      721 ttgtggtagc caaacaagca cattgacagc aactgttagc ggtactttcc ctacaggttc
      781 ttcaattggt tggtatgcaa caccgtttgg aggattacct ttggcaacag gaacaacatt
      841 tacaactcct gttttaactt caacaacaac gtattatgtt ggaaattgcc ctggaacgtt
      901 tagaaaacca gtgcaagtag ttgtggggca aaacccaatc atcgctggaa cagcagttat
      961 taccaatgct acttgtacaa caggtggttc tattactggt ttatcagctt ctggtggaac
     1021 aggcgcatta acctatgctt ggaacggaac agctgctgct ggaccaacat tatccaatgc
     1081 atcagcaggt tcatacacat tgactgtaac agatgccaac ggatgttcat cgcaaagtgg
     1141 accgcataca ataactgggg tgggtggacc aaccattaca ggaacaccaa ccattaccaa
     1201 tcaaacatgt ttagttaacg gaacaatcac ggggttaaca gtaaactctt cggttgctat
     1261 tacttcctat gattggaacg ggacaacatc ggttgaccaa aatttatccg ctacagcagg
     1321 ttcttatacc ttgactgtta ttgatgccaa tggatgttcg gctcaaagtg gaccgtatac
     1381 aataggatta gctccaactc caacaatttc gggtacagct tcaattacca atgccaattg
     1441 taataatggt gggaatatca gcggtttaac agcctctggc ggtgttggaa cattgaccta
     1501 tgattggaat ggtgcaactt cggtaacaac tgatttatct aatgcagcgg caggttctta
     1561 tacattgact gttaccgatg ctaacggatg tacagctcaa agtggaccac atcaaattaa
     1621 tacggatgcc aatccaacca tcactggaac agcagttgta accgatgcga catgtactgt
     1681 gcaaggttct atcaccggat tatcagcaag tggaactgga acattaacct atgattggaa
     1741 tggaacacct tcgccttcaa ttgatttaac aggcgctgga gtaggaagtt acacgcttac
     1801 cgttacagat ggaaatggat gtacaacaca aagcggacct tatactatta acgtaaccgg
     1861 tggaccaacc atctcaggta cagctgttat ttcggatgtt acttgtacaa cccaaggttc
     1921 tattacaggt ttgactgttt ctggaggcac aggtacttta acatatagct ggaacagtac
     1981 tatttcacca acagcagatt taaccaacgc aacggctggt acatacaccc tttctgtggc
     2041 agatgccaat ggatgtattt cgcaaagtgg accatacacc atcaacacaa cacctgcacc
     2101 aaccattgct ggaacaccag taattaccga tgcaacgtgt acaacaggag gttcaattac
     2161 cggaattacc gtttctggtg gtgcacaacc atatacctat caatggaata acaatgcagc
     2221 atcaggaatt gatttaataa atgtgtctgc gggaacatac accttgagta taacggatga
     2281 caatggatgt gttgttacaa gcggacctca caccatagga agtcaaggag ctgttacaat
     2341 ttctggaaca gcaactgtaa ctcatgcaac ttgtacaact ggtggttcta tcacaggttt
     2401 aacggcaacg ggtggtgcaa acccatatac gtttgaatgg aataatgtta ctactccatc
     2461 tgcagattta accaatgcgc aaccaggaag ttatgttttg gaagtaacag atgctaatgg
     2521 ttgtattgca acaagtgctt cttacaccat taatgcaccc aatactccaa caattggaac
     2581 aactgcaacc atcgtggatg caacatgtaa ccaaggagga tcaatttcgg gagtggtagt
     2641 ggccaacggt acaggaccgt atcaatattc ttggaacaat ggccaatatt caacattaga
     2701 tatcacaaac gtacctgctg gctcatatac attaacagta acagatgctt cgggttgttc
     2761 agtaacagga accccattga cggttggaca aacagctgct gttgtggcac aatttaccta
     2821 ttctcccaac cctgttatga tcaatgagaa cattcaattt caagatgaat ctacaggaaa
     2881 tgttgtggca tgggaatggg ttattgaaca agacacgatg accattcaaa acccaacaca
     2941 tacctttgaa aatgatggaa tgtatgatgt aagtttggtt gtagtggatg caaatggttg
     3001 tgttgataca gcaaaagtaa cgattgaagt aatttctgaa atggaagttc caaacgtcat
     3061 aacggttaat ggagatggaa ttaacgattt atttgaaata aaaggcttgt tgcccgaaac
     3121 aaaagttatc atcttgaacc gttggggaga attgatttat acatcggata attatgcaaa
     3181 taactgggat ggtaaagata agtcaggaaa aacggtaaca gagggagttt acacctattt
     3241 agtgttagca aaagatggaa gtaaaaaaca tggatttgtg catgtagttg tcaaacatta
     3301 aatatttttt tgtaaattaa cctcgaaatt taaccatgtt agacactaaa aaatatgcaa
     3361 gcacttctat tggtatgatt taccaaggat atttgcatga gtatgaggaa gaagaaaaac
     3421 aaaaagtcat acttaaattt tttggtgagt ttaccatcaa tctaattcga aattttgccg
     3481 atggaattga aaacctaatg gcaaaagaag gcgataaacg acacgtggta aaacgaatgt
     3541 ttagcattct tatcgaggga ttgcaaaatg cacacattca tggtggtaga gacgaagagg
     3601 gagaacaacg ttcgttttta atcatctcac aaaatccgga acaatacaaa attacttttg
     3661 gcaatatcgt tgaacaaaaa gacaaggaat tggtaaccac ctatttgggt aatatcaata
     3721 accacaatga agaggaactc aaacaattat acattgccat cctaaaaaat ggttatttat
     3781 cgcataaagg tggtgccggt ttgggtattg taaccttgcg tatgaaatct tccaaattaa
     3841 ttaattaccg catttatgaa ctttccagct caaaatattt tattgtaatg gaagtttgtc
     3901 ttgacagaaa ttaaaacgat gttctaaaaa attctgaaag caaaatactc gttgcaatag
     3961 aaacattgag cgattctgtt tgccctttct ttggaatagt caatggtttc gaaataaacg
     4021 gttttaattc atccgagatg ccatttcctt cattgcccat caataaaatg ccgtgaggtt
     4081 ttaatggttg agcataaacg ttttttccct ccaacaaagc tccgtaaaca ggtaaatcgg
     4141 tagttgcaaa aaattctttt aaatcaaggt attcaacctg aatgcgcaaa aatgccccca
     4201 tagtagcttg tattactttg ggattgtaaa tatctaccgt tgtttttgag caaatcactt
     4261 tttgcactcc aaaccaatca gctaaacgga gtatggttcc catatttcca ggatcttgca
     4321 ccgaatctaa agcaatcata aagttgggag aactattttt gaactccatt ttttttacga
     4381 ctgccaaaac tttgttgggc gttgtaagcg atgaacattt ttttaaagta gcttcatcgc
     4441 aatgatactg tgggatagtg ttcgtagtta cctcaaattc aggagttgca acaaggtact
     4501 caatttgctg aggtgcaaaa tcaattaatt cagcaacaat tttttctcct tcaacaacaa
     4561 ataagccttg tttatcgcgg catttttttt gttgcaaaca aatgatttgt tttatcttat
     4621 taagagataa gggttctgcg ttcacttttt attatttttg tttcgaaatt acacatttga
     4681 gtcgatttaa acacatatca ttcctaataa ttagcattgc ttttcttcta agtagttgca
     4741 aacaaacgaa attcgttcca gaaggcaaat atttattaaa gaaaaacaat gttatagcta
     4801 ataaaagcga gattgctaat tacgaatatc acgaggtaat caaacagcaa cccaactata
     4861 aaaacctagg ttttaaaata aaattagcca tgtacaatgc tgttgactct gcaaaagtat
     4921 cggataaacg cattcgtaaa aacgaaaaat taaaacttaa aaatcaggaa cgtagagcca
     4981 aagaaaaacg cattaacgaa aaacgcattg caaaggcaaa acgaaaaaat agagaatggt
     5041 ataccaaaaa agttattcca ctcaaagata cgctcaaccc taaaatgttt ttcagagaat
     5101 ggttgaaata taaatttggg gaacctcctg ttatttttga taccgcctac ttgagtcagt
     5161 ctataagtca attggattta ttcctcaaga aaaaaggata ctactacagt gaagtcactg
     5221 gtgattatac aacaaaaggt aaaaaagcgg ttgccaatta ttatattaaa acaggcaaac
     5281 gttattacat tgattcggtt tatgttgccg gaccaaattt gagtgttaat caatcgtatt
     5341 tgaattttat tcgacgtggt aagatagaat cgcttgaaaa acaaccgttt gatagtgatt
     5401 tattgaatga ttatcgcacc gatgttgctc gtcttatgcg cgatgaagca ttttttggat
     5461 tcaatttttc gagtattgat tttgtagccg atactgcaaa aggagattac aaagttacgt
     5521 taggcatccg ctttatggat agaatgatta aatcaccgga ttatgcggat agtttttaca
     5581 gtgttccata caaaacgtat ttagtaaaaa gtgttcattt tcatattttg gataccaccc
     5641 gaatgaaagg aaattacgat tatcgaatga aacaattagg tcttgttcca ccaaaaaatg
     5701 gagaaattgt aacaatggat acgctgttgt ttgataacat ttattacaat cgttctgaaa
     5761 aaaagagaag aggttttcca ctcaatgaaa aagtgaaaaa tccattgcga atggcaagtt
     5821 tttactataa cggaaaaatg tttgtgcgtc caggaattat tgaaactcaa aattatcttg
     5881 aaaacaaaaa ttattacaaa gaatattatt tagaacgatc gtattctcgt ttattgcaat
     5941 tggatatatt ctccagcgtt aagcctaaat tggttgaact tcaagattcc agtcaagttg
     6001 aggtgcatta ttacctcaca cctgccaaac gccagtactt tgcttttgag ccaaagttta
     6061 ccaactccaa cggttttttg ggactttctg cgagtatcaa ttatagcaac agaaatattt
     6121 ttagaggagc agaaaaatta accttttcat tgggaggagg ttttgaatcg caaccaccta
     6181 tttttgatga atccatcaat ggtgaaaaaa ttaaaaaagc gggtcgaagt ttcaacacct
     6241 ttgaaattga accgggtata aaactagaaa ttccaggtat tttcccgttc agaaaagcag
     6301 cttttatggc aaaacgccaa cgtgctagaa ccgtcatcac tacatcattt aactatcaaa
     6361 acagaaccga ttttaaacgt agaagttttc aatttgggta tttgtggcgt ttttatggtg
     6421 ccgaaacgca aatcttccaa ataggattgc ctaccacctc gtcggtgaaa tatgtgttgt
     6481 ttaatccaag tgcccttttt gaacaaaaat taattgaatt aaacgatgtg ttcttgttca
     6541 atacctatag cgaccaattt atttggca
//
LOCUS       DAOQVQ010000086         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13022_length_6518_cov_3.669194, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000086 DAOQVQ010000000
VERSION     DAOQVQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13022_length_6518_cov_3.669194"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKN22_03965"
     CDS             complement(<1..209)
                     /locus_tag="PKN22_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNS41890.1"
                     /translation="MATIKLKDFEVGYTDKFFFDTNIWLLLYGTVAGFQANDQSQYAK
                     LLENLITRKSPIYITSMVVSEFANV"
     gene            complement(196..549)
                     /locus_tag="PKN22_03970"
     CDS             complement(196..549)
                     /locus_tag="PKN22_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HNS41891.1"
                     /translation="MKHIIIKDVINSELAVSTENGMKVFEIVDSCLQRREKVELDFAG
                     ITIMITAFLNAAIGSLYSKKEYTGEFLNEYLKLENVEMDDRILFRDVIQRAKEYFADK
                     ESFEKNSNDALYGND"
     gene            complement(579..1460)
                     /locus_tag="PKN22_03975"
     CDS             complement(579..1460)
                     /locus_tag="PKN22_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41892.1"
                     /translation="MIITIPNDIKSEFVGYDFFITWINEHQNVRNSEITFDFEKVTFL
                     EANLCALIGAIFEILESNNNKISLKNINTAIETILRKNAFLVPFGYERIDDNYDTSLT
                     YMKFTPNDDSGFYNYIQNELLSKQQFPSHSEKLGKEITRNIFEIYENARTHGRCDYIH
                     TCGQFFPRKVDKPLHFTIVDKGVNIKQNVSDYLNKEIGGADAIEWAMIKGNTTKTGET
                     SGGLGLSVIFDFIKLNRGKIQIISSDGFYEFKNGNVVKAKLNSIFEGTIVNIKFNLND
                     SNHYMLIQEVEDFDNIF"
     gene            complement(1712..2275)
                     /locus_tag="PKN22_03980"
     CDS             complement(1712..2275)
                     /locus_tag="PKN22_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNS41893.1"
                     /translation="MIKKTFLIASLGVITLFASCNQSATTANPTEKTAAANVITQNLK
                     DKDGNAIQLIFDNNKDEVTINYKNETSVLKSQKAASGMWYKNDSYELRGKGNDLTLAK
                     GDQIIYEHADEIVEIVAKSNNGDVLNMTFNNTAGTVKAYLNGGEQIDLEEQKAASGIW
                     YKNDHYELLGKGDHYQLLKDGKTVFSN"
     gene            complement(2407..3237)
                     /gene="ygiD"
                     /locus_tag="PKN22_03985"
     CDS             complement(2407..3237)
                     /gene="ygiD"
                     /locus_tag="PKN22_03985"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNS41894.1"
                     /translation="MKNLNDLYRSSLTLPNTERMPVLFLGHGSPMNAIEENEFVNGFR
                     TIAKTLPLPHAILCVSAHWFTKGTKVTAMEMPRTIHDFGGFPKELYEVSYPAKGSPEL
                     AQITKDLLVPTNVELDEKWGLDHGAWSVIKHLYPAANVPVIQLSIDYTQPAHYHFELA
                     QQLSTLRNKGILIIGSGNIIHNLRLVDFKNMDKDNYGYDWAIEAHQFVNQHILDGNYS
                     PLIHYEKQIKALQLAVPTPDHYLPLIYALGLQQKNEQISLFNDKLMAGSLSMTSLKIG
                     "
     gene            complement(3340..3774)
                     /locus_tag="PKN22_03990"
     CDS             complement(3340..3774)
                     /locus_tag="PKN22_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS41895.1"
                     /translation="MATVNVYLNFKGNCKEAFDFYKSVFGGEYPYIGTFGEMPPSNDM
                     PPIAPEDKDKIMHVTLPISNETCLMGSDTIGVHSNDFTMGNNYAVSINANSRQEADQL
                     FDGLSNGGVVTMPMTNTFWGAYFGMFTDKFGINWMVNYDEQP"
     gene            complement(3811..4407)
                     /locus_tag="PKN22_03995"
     CDS             complement(3811..4407)
                     /locus_tag="PKN22_03995"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNS41896.1"
                     /translation="MKLLNFHRLELIYFLLFTLWGVANGQVTVFYLIYLFWFQELIRT
                     IVDLFFIIKQQKTIAERWFSAKNSFGGFFILFIYFVFIVVLFGWMVNFKNSELLATNI
                     GVFVFKNWYFNSTLILFFIEYLYFRTQVDNSNLKIEIFNKRHIILHISIIAGAIIQMI
                     LLPKLNIDNQWGNALVVLPFLLLKLFLNSQSDFKTNKI"
     gene            complement(4432..5235)
                     /locus_tag="PKN22_04000"
     CDS             complement(4432..5235)
                     /locus_tag="PKN22_04000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS41897.1"
                     /translation="MNNEENYIDINRQSWNKRVDAHLNSDFYDLERFMAGKNSLNDIE
                     LNLLGDIKGKSILHLQCHFGQDTISLSRLGAEVIGVDLSDKAIDSAIKIANETNSTAK
                     FICCDIYDLPHHLDEKFDFVFTSYGTIGWLPDLDKWAKIISKFLKPGGQFIFVEFHPV
                     VWMFDDNFDKIAYRYFNSGAIVETQNGTYADKSAAITITEISWNHSISEVVNSLINNG
                     LEINALDELDYSPYNCFNKTIEFEPNKFRIKHLENYLPMVYSIKATKKY"
     gene            complement(5285..5716)
                     /locus_tag="PKN22_04005"
     CDS             complement(5285..5716)
                     /locus_tag="PKN22_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41898.1"
                     /translation="MKKATFYLIPLFTLILISSCTKEKPPVGNYIGVFTYESPTYSAD
                     KTIWLKIVETNKDYILIGETDYHGNLTSVWFDTLYKKGKKDIEGNVPALSNGPTYIKG
                     KWSRNLFSNKYTIKGDFTDTYYAQGGSKQFQNKGTFEIKSN"
     gene            complement(5811..>6518)
                     /locus_tag="PKN22_04010"
     CDS             complement(5811..>6518)
                     /locus_tag="PKN22_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41899.1"
                     /translation="YLKFMATKFNVYKPILPLLEKCLKKSNQQHWVDLASGGGSGLIC
                     LAKDLKNVHPDLKITLTDYYPNIKAFEKLQEEVPHTFLYEKSSVDASNPPQHLQGTVQ
                     TIFGAFHHFRPDKAKSILQNAVNTQSTIAIFEPVGRNFGSWFSMLFVPLNVLIFTLFI
                     RPVRWNVLPFIYLVPIIPLYILWDGIASILRTYSEQELQELVNSLENKEEYVWEIGKT
                     AGPMPIHYLLGYKKAEI"
BASE COUNT         2125 a         1093 c          991 g         2309 t
ORIGIN      
        1 aaaacatttg caaactcaga aacaaccatt gaagtaatat aaattggtga ttttcttgtt
       61 attaaatttt caagtaattt agcatattga gactgatcat ttgcttgaaa tcctgcaact
      121 gtaccatata ataaaagcca aatatttgta tcaaaaaaga atttgtcagt ataaccaact
      181 tcgaagtctt ttaatttaat cgttgccata caaagcgtca ttagaatttt tctcaaatga
      241 ttctttatct gcaaaatatt cttttgctct ttgaataaca tctctaaaaa gtattctgtc
      301 atccatttct acattctcaa gtttcaaata ttcatttaaa aactctcctg tatattcttt
      361 tttactatat aaacttccaa ttgcagcatt aagaaaggct gtaatcataa tcgtaatacc
      421 agcaaaatct aattcaactt tttctcttcg ctgtaaacaa gaatcaacta tctcaaaaac
      481 tttcattcca ttctcagttg aaaccgctaa ttctgaatta attacatctt ttattattat
      541 gtgtttcatt gtttttttct ttatacaaat gtacgttatt aaaaaatatt atcaaaatct
      601 tcaacttctt gaattagcat ataatgatta gaatcattaa gattaaattt aatatttaca
      661 atggttcctt caaatataga atttaattta gcttttacaa catttccatt cttaaattca
      721 taaaaaccat cagatgaaat aatctgtatt ttcccacggt ttaatttaat aaaatcgaaa
      781 ataacagata atcctaaacc tccagaagtt tcacctgttt tggttgtgtt acctttaatc
      841 attgcccatt caatagcatc agcacctcca atttctttat tcaaataatc agaaacattc
      901 tgctttatat taacaccttt atcaactatt gtaaaatgta atggtttatc aacttttctt
      961 gggaaaaatt gaccacaagt atgtatataa tcacatcttc cgtgtgttct agcattttcg
     1021 tatatctcaa aaatatttct tgtaatttct ttccctaatt tttcactatg agaaggaaat
     1081 tgttgtttac ttaataattc attttgaata taattataaa aaccactgtc atcatttgga
     1141 gtaaacttca tataagttaa tgaagtatca taattatcat ctattctttc ataaccgaaa
     1201 ggaactaaaa aagcattttt tcttaaaata gtttctattg cagtatttat attttttaaa
     1261 ctaattttat tattatttga ttccaaaatt tcaaaaatag caccaattaa agcacataaa
     1321 ttagcttcta aaaaagttac cttctcaaaa tcaaaagtaa tttcagaatt tcttacattt
     1381 tgatgttcat taatccaagt tataaaaaaa tcatatccta caaactcact tttaatatca
     1441 ttgggaatag ttattatcat ttagtggaaa cttaattaat atttttgcta aaatataaaa
     1501 aaatagacaa atttaaatta aattactact gctaacatcc gtttttcgct attgcaagta
     1561 aagttcaaaa ttcacgtttt tctttcgcaa gaattattat ctatagctga aaatttagtg
     1621 ctccgaatct cgcaacagac gcaaagcggc gaaccgttca aaagtaaaga aaacaaaata
     1681 taaaaggttt agttgctaaa aatgggatag attagttgct aaaaactgtt tttccgtctt
     1741 ttaaaagttg gtaatgatcg cctttgccca aaagttcgta gtgatcgttt ttataccaaa
     1801 ttccagaagc tgctttttgt tcttcaaggt caatttgttc gccaccattc aaataggctt
     1861 taaccgtacc ggcagtattg ttaaaagtca tgttcaacac atcgccattg ttactttttg
     1921 ctacaatttc tacaatttca tccgcatgtt cataaatgat ttggtcgcct tttgccaaag
     1981 tgaggtcgtt tccttttcct ctcaattcat agctgtcgtt tttataccac attcccgaag
     2041 ccgctttttg acttttaaga acagatgttt cgttcttgta gttgatagta acttcatctt
     2101 tgttattatc aaaaatcagt tggatggcat ttccatcttt gtcctttaaa ttttgagtaa
     2161 taacgttggc agcagctgtt ttttctgttg gatttgcagt tgttgcactt tggttacatg
     2221 aagcaaagag tgttatcact cctagagaag caattaaaaa agttttttta atcattttga
     2281 gttaaattga atgttattac taatgttgtg cttgattttg ctgtaaaatc gagttaaaat
     2341 taatggattt tctaattaat aagttgaatt ttaaacaata tttaacgttg ttgatgattg
     2401 ctccatttac ccgattttca aactggtcat gcttaaacta ccagccatta gtttatcgtt
     2461 aaaaagacta atctgttcgt ttttttgttg caagcccaag gcataaatca acggtaggta
     2521 atgatcggga gttggaaccg ccaattgcaa tgcttttatt tgtttttcgt agtgaattaa
     2581 tggtgaataa ttgccgtcca gaatgtgttg gttcacaaat tgatgtgctt caatggccca
     2641 atcgtagcca taattgtctt tatccatgtt tttaaaatca accaatcgca aattgtggat
     2701 gatgttgcca cttccaatga ttaaaattcc tttgttgcgc agggtgctaa gttgttgtgc
     2761 gagttcaaaa tggtagtgcg ctggttgggt gtaatcgatg ctgagctgga tgacaggaac
     2821 attggctgct ggataaaggt gtttgatgac actccaagcg ccgtggtcga gtccccattt
     2881 ttcgtccaat tcaacattag tcggcactaa caaatcttta gttatttgtg ccaattcggg
     2941 cgaacctttg gcgggatagc ttacttcgta aagttctttt gggaaacctc caaaatcgtg
     3001 aatggtgcgg ggcatttcca tggctgtaac cttggtgcct ttggtaaacc aatgtgccga
     3061 aacacacaaa atagcatggg ggagaggcaa tgtttttgca attgttctaa agccgttcac
     3121 aaattcattt tcttcaattg cgttcatcgg gcttccgtgc cctaaaaaca aaaccggcat
     3181 tcgctccgta ttgggaagtg taagtgatga acggtataaa tcgttgaggt ttttcatact
     3241 caaaagaaaa ggtgtcaacc cgatgatttt taaaaatgtt tttctatcca ttattgtcct
     3301 tttgacaagc agaattgaga attaatcgga aagcaactct taaggttgtt catcgtaatt
     3361 gaccatccaa ttgatgccaa atttatcggt aaacattccg aagtaagctc cccaaaaagt
     3421 attggtcatg ggcatagtaa caacaccacc gtttgacaaa ccatcaaaaa gttgatctgc
     3481 ttcttggcga ctgtttgcat tgattgaaac cgcataatta tttcccattg taaaatcatt
     3541 gctatgcaca ccaattgtgt cacttcccat taagcacgtt tcattgctaa ttggcagtgt
     3601 tacatgcatt attttgtcct tgtcttctgg agcaatagga ggcatatcat tgcttggagg
     3661 catttctcca aatgtaccaa tgtagggata ttctccacca aatacagatt tgtaaaagtc
     3721 aaaagcttcc ttgcaatttc ctttaaaatt tagataaacg tttactgttg ccatactttt
     3781 tattttatgg ttaaagatag tgaaaaaaca tcaaatttta ttggtcttaa aatccgattg
     3841 ggaatttaaa aatagtttga gcaacaagaa tggtagcaca acaagcgcat tgccccattg
     3901 gttatcaata ttaagttttg gtagcaaaat catttgaatg attgctccag ctatgataga
     3961 aatatgcaat atgatatggc gtttgttaaa tatttcaatt tttaaattgg agttatcaac
     4021 ctgtgttcta aaatataaat attcgataaa aaacaagatg agtgtgctgt taaaatacca
     4081 atttttaaaa acaaatactc caatattagt tgctaataat tcggagtttt taaaattcac
     4141 catccatcca aacaagacaa caataaagac aaagtaaata aaaagaataa aaaatccacc
     4201 gaatgaattt ttagcagaaa accaacgttc tgcaattgtt ttttgttgtt ttataatgaa
     4261 aaataggtca acaatggtgc gtatcaattc ttgaaaccaa aacaaataaa tgagataaaa
     4321 cacagttact tgtccgtttg caacacccca aagtgtaaac aacaagaaat aaatcaattc
     4381 taatcggtga aaattaagta gtttcattgt taacaattaa cgaattttgt ttcagtactt
     4441 ttttgttgct ttaatagaat aaaccattgg caagtagttt tctaaatgtt tgattctgaa
     4501 tttatttggt tcaaattcta ttgttttatt aaagcagtta tagggagaat aatccaattc
     4561 atctaaggca ttgatttcta atccattgtt aattagacta ttcacaactt cgctgatgct
     4621 gtggttccat gaaatttctg ttattgtgat tgctgcagat ttgtcggcat aagttccgtt
     4681 ttgtgtttct acgattgctc cagaattaaa ataacggtat gcgattttat caaaattatc
     4741 atcaaacatc catacgacag gatgaaattc aacaaaaata aattgtccgc ctggctttaa
     4801 aaattttgaa attattttag cccatttatc caaatctggc aaccatccaa tagtgccata
     4861 acttgtaaaa acaaaatcaa acttttcatc taaatgatga ggtaagtcgt aaatgtcaca
     4921 acaaataaat tttgcagttg aattagtctc attcgcaatt tttattgcac tatcaatagc
     4981 tttgtcagac aaatcaacac ctataacctc tgcaccaagt cgacttaatg aaattgtatc
     5041 ttgtccaaaa tgacattgca agtgaagtat tgattttcct ttgatgtctc ctaataagtt
     5101 taattcaata tcgtttaatg aatttttacc agccataaat cgctctaaat cataaaagtc
     5161 agaattcaag tgtgcatcaa ctcttttatt ccaagattga cggttaatgt cgatgtaatt
     5221 ttcttcgttg ttcataattg tttgtgatta agaaaaaaat aggtattagg atttaacttg
     5281 ttacttaatt agacttaatc tcaaacgttc ctttattttg gaattgtttg ctgccacctt
     5341 gcgcataata ggtatcggtg aaatcacctt ttatcgtata tttattacta aacaaattgc
     5401 gcgaccattt tccttttata taagttgggc cattacttaa tgcagggaca tttccttcaa
     5461 tatctttttt accctttttg tatagcgtat cgaaccacac tgaggttaaa tttccatgat
     5521 aatccgtttc tccaattaga atataatctt tattagtttc cacaattttt aaccaaattg
     5581 ttttgtcggc tgaataggtt ggactttcat aagtaaatac cccaatataa ttacccacag
     5641 gtggtttttc ttttgtgcaa ctactaataa gtattagtgt aaaaagtggg attaaataaa
     5701 atgtagcttt tttcatatct gcaattttaa tagcatggat tactatcaat aagacaaata
     5761 tagaataaaa acttttgcct ctcattcaac aatattcatt ttctgtcaga ttagatttct
     5821 gcttttttgt aacccaaaag ataatgaata ggcattggtc cagcggtttt accaatttcc
     5881 caaacatatt cctctttatt ttcgagggag ttaaccaact cctgcaattc ttgctcagaa
     5941 taagtacgca aaatagaagc aatgccatcc cataaaatgt aaagtggaat gattggaacg
     6001 agataaatga atggcaatac gttccagcga actggacgga taaacaaagt gaaaatcaat
     6061 acgtttagtg gtacaaataa catggaaaac caacttccaa aatttctgcc cacaggttca
     6121 aaaattgcaa ttgttgattg cgtatttact gcattttgca atatactttt tgctttgtcg
     6181 gggcgaaaat gatgaaaagc accaaaaatt gtttgaacag tgccttgcaa atgttgagga
     6241 ggattgctcg catcaaccga tgatttttcg tataaaaaag tatggggtac ttcttcttgt
     6301 aatttttcaa aagctttgat gttgggatag taatcagtaa gagtgatttt taaatcggga
     6361 tgtacgtttt ttaaatcttt tgccaaacag attaaaccac taccgccacc agatgctaaa
     6421 tccacccaat gttgttggtt gctttttttt aagcattttt caagcaacgg caaaataggt
     6481 ttgtagacgt tgaattttgt agccataaat tttaaata
//
LOCUS       DAOQVQ010000087         6473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13149_length_6473_cov_4.037083, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000087 DAOQVQ010000000
VERSION     DAOQVQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13149_length_6473_cov_4.037083"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..502)
                     /locus_tag="PKN22_04015"
     CDS             complement(77..502)
                     /locus_tag="PKN22_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNS41900.1"
                     /translation="MKESPLESLVVSVKINKTISDVWNAWSNPELMPNWLVIYDNFEA
                     KIIKFEFRTGGDSNYIMASKDGKLNYAYNSIISLIIPFEKIEYHLEDGRTAILSFSVT
                     KHQTMVQMEFEPVAHQDVGAQLFAWQTILDNFKNYLERE"
     gene            complement(615..1484)
                     /locus_tag="PKN22_04020"
     CDS             complement(615..1484)
                     /locus_tag="PKN22_04020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS41901.1"
                     /translation="MSQKKMFTEGWADYELIDAGGGKKLERWGKIITIRPEIQAYFKS
                     EIPFEKWYQMAHWEFIQKGSSGKWKKLQENAPQEWTIAYKHLKFKLSLTKFKHVGLFP
                     EQRTNWDYINKHITSDDRFLNLFAYTGAASLVARYNGAETLHVDSVKPIVSWAKENME
                     LSRLLNVKWVVEDALKFAQREEKRGNTYKGIIMDPPAWGIGAKGEKWKLEEKIDELLS
                     SAANLIEENGFLIVNTYSPQLTLDELKGLAELYFSENTYDIKELWMKSTSGKELYFGN
                     ILRVSFTSDSNEK"
     gene            complement(1490..2068)
                     /locus_tag="PKN22_04025"
     CDS             complement(1490..2068)
                     /locus_tag="PKN22_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNS41902.1"
                     /translation="MIGFIIKCVLALTSLLGTISLFASGHWGWGIVMIFVTALIGVFF
                     FRHERIIMAFYYMRLGNQQKAKYHFDKITAPQFLPKKQRAYVLYMQAVLGSQENGPAK
                     TEASLRKALAMGLRAKHDNAIAKMHLAGACAQTGRRQEALTLLAEAKKLDDSGMMKDQ
                     IKMLQDQLKMAPSANQMRMAQMMGGRRKMPRR"
     gene            complement(2081..2512)
                     /locus_tag="PKN22_04030"
     CDS             complement(2081..2512)
                     /locus_tag="PKN22_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNS41903.1"
                     /translation="MDPSKISTNKAPKPVGAYPHARKVGNLLFLSGVGPRTAGSDAND
                     SGVPGLELDKNGNFLAFDFEAQCRSVFDNVRVILEEAGSSWDQLVDVTVFLTNMKRDF
                     HTYNKLYNEYFKDNQPCRTTVEINSLPTPIAIELKCIATID"
     gene            2616..4826
                     /locus_tag="PKN22_04035"
     CDS             2616..4826
                     /locus_tag="PKN22_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS41904.1"
                     /translation="MKRFHLYLFLFLGAISVHFTVFSQLDIQAFNASLKKVNTPSKKF
                     QILLSNAKLYQTSNVAFADSLKDVLVSTSIVLDDSSRVAALLFKADMEQITGNRVAYF
                     STVLSCQPFINRINSDKIQIEIFKHLSNYHILNKESDMAFFYLKRMLRIAHDINSKNY
                     VAEANLLMSKNYANNLHLKDSALFYINLALQNVRRTSDKSLLADCFHQQALTYKTFGQ
                     TDLSASKDILALQIASEIKNLRQIALFSLEIGRAQLAIHNTKDAEYYFNQALENGIRI
                     NDKNLEALSLIELANSYFIKKKLNEAKRNCLNAIAIFETLNNKDGLGNAYNTLGIIYR
                     EQKEYNKAGTQFNNALVNYESVGNMNQIALVYQNVATVFYANKKYQNALNYLLRSNEI
                     SKTNSTEIPMFENYRLISKIYKELGQTQKALEYMQLYVELKDSNALRQSVSKIAELNE
                     LYRAEQRDRLILSQADSIEKQRQQRQLTATQFENVKLRNTFQIYVIIGFILLIIMAYI
                     ILRNRWKQNEIKQQQREAEMAQTLLRAQMNPHFVFNAMSVIQSYIYENDTKNSAKFLV
                     NFSRLMRLILENSPKEEIPIQLEVEILKKYLETQKMRFENRFDYTIEVTDELTFEKAL
                     IPPMITQPFIENAIEHGQLHTVENGFIHILFRKKIHMLEIIIEDNGVGRKTSEKNKKS
                     REHKSMAMKITKERIENLNRKYKTEGFLKIEDYDKTLQTGTKILISLPYRVEAV"
     gene            4839..5585
                     /locus_tag="PKN22_04040"
     CDS             4839..5585
                     /locus_tag="PKN22_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS41905.1"
                     /translation="MKKKALVIDDENRTRDLIAKLINSFELDIEAISEGESVKSGLKA
                     IEKHEPDIVFLDIQMPDGSGFDILKALPKKSFEVVFITAHEEFAIKAIKFSALDYILK
                     PVDADELKEVVERALEKMNTKNDERQFEALQHNISPQQKRRLVLKTQESVHVVDLDQI
                     IRLESDRNYTSFYLNDGKSILVSKTLKEYDDLLAGYNFIRVQQSHLINLDYVEKYNKG
                     NGGFVVMKDGSEVPLSPAKREIFFKIIENL"
     gene            5603..>6473
                     /locus_tag="PKN22_04045"
     CDS             5603..>6473
                     /locus_tag="PKN22_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNS41906.1"
                     /translation="MSIRFKIIVSCAVLLFAISVHLFAGSIAISFNEYWNALFHYDAA
                     NTHHVIARDLRIPRILMAIIAGAGLSLAGLLMQTIFYNPLAEPNILGVSTGSSLFVAI
                     ALMTGFTFFNSDFGLISSALFGALIFGVLILFFARFVRSHGSLLLIGIMLGSFSAAFI
                     SIIQTWSDAHQLKLFTLWAMGSLQQVTYAQLPLIFLLFSSSIVATFFLVKALNALVLG
                     QENAAVLGVNSRNTRMLVILIGSVLTGVITAFCGPIAFVGMAVPNLVKLIYKTQNHRI
                     LIVATLVTGSIFMV"
BASE COUNT         2103 a         1174 c         1098 g         2098 t
ORIGIN      
        1 ttaaaggtta ataaatacaa aaaagtcgga agaaatttct tccgaccatt cgtggctcaa
       61 aagcgacttt tttgttttat tcacgttcca aatagttttt aaaattatcc aatattgttt
      121 gccaagcaaa aagttgagca ccaacatcct gatgggcaac aggctcaaac tccatttgca
      181 ccattgtttg atgtttagtc acagaaaaac ttaaaatagc tgtacgtcca tcctcaagat
      241 gatattcaat tttttcaaaa ggaataatta atgaaattat actgttgtag gcatagttca
      301 atttaccatc cttggaagcc atgatataat tagaatcgcc gcctgttctg aactcaaatt
      361 tgataatttt tgcttcaaaa ttatcgtaaa tcacgagcca attaggcatc aattctgggt
      421 tggaccaagc gttccaaacg tcgctaatcg ttttgttgat tttgacgctt acaaccaaag
      481 attctagagg actttctttc ataagttatt tgagtggtat ctattcgttt agaacaaata
      541 taacatttaa cctattgaaa gcaagaaaga ataaaaataa aattgttaaa aaataaactt
      601 acctttcaat gacactattt ttcgttagaa tcactggtaa aagaaacacg taaaatattt
      661 ccaaaataaa gttccttacc actggtgctt ttcatccaca attctttaat gtcataagtg
      721 ttttctgaga aataaagctc tgctaaaccc ttcaattcat ccaatgtaag ttgtggagaa
      781 tacgtgttga caattaaaaa accattttct tcaatcaaat tagctgcaga tgagagtaat
      841 tcgtcaattt tttcttccaa cttccatttt tcaccttttg caccaattcc ccaagctggc
      901 ggatccatga ttattccctt gtaggtattg ccgcgttttt cttcgcgttg tgcaaatttc
      961 aaagcatctt ccaccaccca ttttacattg agcaagcgtg aaagttccat gttttccttt
     1021 gcccaactca caattggttt tacagaatcc acatgcaagg tttctgcacc gttatagcga
     1081 gccactaaag atgcggcacc tgtgtaggca aataaattta aaaaacgatc atccgatgtg
     1141 atgtgtttat tgatgtaatc ccagttggtt cgttgttcag gaaaaagtcc aacatgttta
     1201 aatttggtga gcgatagttt gaatttcaaa tgcttgtagg caattgtcca ttcttgaggg
     1261 gcattttctt gaagtttttt ccactttcca ctacttcctt tttggataaa ttcccaatgc
     1321 gccatttgat accatttttc aaaaggaatt tcacttttaa aataggcctg aatttctgga
     1381 cgaatagtga taatttttcc ccaacgttct aattttttgc cacctccggc atcaattagt
     1441 tcataatcag cccacccttc agtgaacatt tttttctggc tcattgtgct tatcttctag
     1501 gcatttttct gcggccaccc atcatttgag ccatgcgcat ttgattggca gaaggtgcca
     1561 tttttaattg gtcttgaagc atcttaattt ggtctttcat catgccagaa tcgtctaatt
     1621 ttttagcctc agccaacaat gtcaatgctt cttgtcgtct tcctgtttgt gcacatgctc
     1681 ctgctaaatg catttttgca attgcattgt cgtgtttagc acgcaatccc atcgccaacg
     1741 ctttgcgcaa tgaagcttca gtttttgccg gtccattttc ttgcgaacct aaaacggctt
     1801 gcatatacaa cacataagca cgttgttttt taggtaaaaa ttgcggtgca gtaatcttgt
     1861 caaaatggta tttagctttt tgttggtttc ctaatcgcat gtaataaaat gccataataa
     1921 tgcgctcatg acgaaagaag aaaacaccta ttagggcagt tacaaaaatc attacaattc
     1981 cccatcccca atgaccactt gcaaacaaac taatagttcc caaaagtgat gtcaacgcca
     2041 aaacgcattt aataataaat ccaatcatag tatatatttt ttaatcaata gttgcaatac
     2101 attttaattc aatagcaatc ggtgttggca gtgagttgat ttcaactgtt gttctgcacg
     2161 gttgattgtc cttaaaatat tcgttgtaca atttgttgta ggtatgaaaa tctcgcttca
     2221 tatttgttaa aaaaacagtt acatccacta attgatccca agatgaacct gcttcctcta
     2281 aaattacacg aacattatca aaaacacttc tacactgagc ttcaaaatca aacgctaaaa
     2341 aatttccgtt tttatccaat tccaatccag gaactcccga atcgttggca tcagaaccag
     2401 cagttcgtgg accaacaccc gaaagaaata aaagatttcc aactttacgc gcatgcggat
     2461 atgcacccac tggctttgga gctttattgg tagaaatttt agatggatcc attttttaaa
     2521 attttagtgc aaagataacc tttttgtgtt ttatacgctt acaattgcat tttgaaaagt
     2581 aaaaattgta attttagctc ttgtaaatta acaaaatgaa acgttttcac ctttatttat
     2641 tccttttttt gggtgccatt agcgttcatt ttaccgtatt ttcacaatta gatatccaag
     2701 catttaatgc ttcattgaaa aaagtaaata cgccttctaa aaaatttcaa atcctactat
     2761 caaatgctaa actttatcaa acttcaaatg ttgcatttgc tgattcttta aaagatgtgt
     2821 tggttagtac aagtattgtt ttggacgatt catcgcgcgt agcggctttg ttgtttaaag
     2881 cagatatgga acaaattaca ggcaatagag ttgcttattt ttctacggta ttgagttgtc
     2941 aaccttttat caaccgcatc aatagtgata aaattcaaat tgagattttt aaacatttat
     3001 ccaattacca tattctcaat aaagaatcgg atatggcttt tttttatttg aaacggatgt
     3061 tgcggattgc acacgatatt aactctaaaa attatgtagc tgaagcaaat ctattgatga
     3121 gtaaaaatta tgcaaacaat ttacatttaa aagattctgc attgttttac atcaatcttg
     3181 ctcttcaaaa tgtgaggaga acttctgata aatcactgct agctgattgt tttcatcaac
     3241 aagcattgac ctataaaaca tttgggcaaa cagatttgag cgcttctaaa gatattcttg
     3301 ccctacaaat agcctctgaa attaaaaatc ttcgccaaat tgcccttttt tctctcgaaa
     3361 ttggacgagc gcaattagct attcacaaca caaaagatgc agaatattat tttaatcagg
     3421 cgcttgagaa tgggatacgc atcaacgata aaaacctcga agcattgtcg ttaattgaat
     3481 tagcaaacag ttattttatt aaaaaaaagc tcaacgaagc caaaagaaat tgtttaaatg
     3541 ccatagcaat ttttgaaacg ctcaataaca aagatggact aggaaacgct tataatacgc
     3601 ttggaattat ttatcgagaa caaaaagaat acaataaagc tggcacgcaa ttcaacaatg
     3661 ctttggtcaa ttatgaatcg gttgggaata tgaaccaaat tgccttggtt taccaaaatg
     3721 tggcaacggt tttctatgct aataaaaaat accaaaatgc gctcaattat ttattacgtt
     3781 ccaatgaaat aagtaaaacc aatagcacag aaattccaat gtttgaaaac tatcgtttga
     3841 tttctaaaat ttacaaagaa ctcggacaaa cccaaaaagc attggagtac atgcaattgt
     3901 atgtagagct taaagatagc aatgcattgc gccaatcggt tagcaaaatt gcagagttaa
     3961 acgaattgta tcgagcagaa caacgcgacc gtttgattct gtcacaagca gattctattg
     4021 agaaacaacg ccaacaacgc caattaaccg ccacacaatt tgaaaatgtg aagctgcgta
     4081 atacctttca gatttatgtg attattggtt ttattttatt gataataatg gcctacatta
     4141 ttttgcgtaa ccgttggaaa caaaatgaaa tcaaacaaca gcaacgcgaa gcagaaatgg
     4201 cacaaaccct tttgagagct caaatgaatc ctcattttgt gttcaatgcc atgtcggtga
     4261 ttcagagcta tatttatgaa aacgatacta aaaattctgc taaattcttg gtcaattttt
     4321 cccgtctcat gcgtttgatt ttggaaaatt cacccaaaga agaaatccca attcaattag
     4381 aggtagaaat tcttaaaaaa tacttagaga cacaaaaaat gcgctttgaa aacaggtttg
     4441 attacaccat tgaagtaacc gatgaactaa cttttgaaaa ggcgctcatt ccaccaatga
     4501 ttacacaacc ttttattgaa aatgctattg aacacggaca attacatacg gttgaaaatg
     4561 gattcattca tattcttttt cgtaaaaaaa tacacatgct tgaaattatt attgaggaca
     4621 atggagtggg aagaaaaact tctgaaaaaa ataagaaaag ccgtgaacac aaaagcatgg
     4681 caatgaaaat aaccaaagaa cgtatcgaaa atttaaacag aaaatacaaa acagaaggtt
     4741 tcttaaaaat cgaagattac gataaaactt tgcaaactgg aacaaaaata ttaatttcgt
     4801 taccctacag agtagaagcg gtttaaaata gaagtactat gaaaaaaaag gcattagtta
     4861 ttgatgatga aaacagaaca cgcgatttaa ttgcaaaatt aatcaactct tttgagttag
     4921 atattgaagc tatttctgaa ggcgaaagcg tgaaatctgg attaaaagcc attgaaaagc
     4981 acgagccaga tatcgtgttt cttgatattc aaatgcccga tggttctggt tttgatatct
     5041 tgaaagctct acctaaaaaa tcatttgaag tagtgtttat tactgcccac gaagaatttg
     5101 ccatcaaagc catcaagttt agtgcactcg actatatatt aaaaccagtt gatgcagatg
     5161 agttaaagga agtggtggaa cgtgcgttgg aaaaaatgaa tacaaaaaat gatgaacgcc
     5221 aatttgaagc cttgcaacac aatatttcac cgcaacaaaa aagaagattg gtactcaaaa
     5281 cgcaagaaag tgtccacgtt gtggatttgg atcaaatcat tcgattggaa tctgacagaa
     5341 attacacgtc cttctatctc aatgatggga aatcaatttt agtgtccaaa acgttgaaag
     5401 aatacgatga tttgctggca ggttataatt tcatccgtgt acaacaatca catttaatca
     5461 acttggatta tgttgaaaaa tacaacaaag gaaacggtgg ttttgtagtg atgaaagacg
     5521 gttctgaagt gccactttct ccagcaaaac gcgagatttt ctttaaaata attgagaatc
     5581 tgtaaattga accaacctac ccatgagcat ccgatttaaa ataatagtca gttgtgctgt
     5641 gctattgttt gccatttcgg tgcacctgtt tgctggttcc attgctattt ctttcaatga
     5701 atattggaat gctttgtttc attacgatgc tgccaacaca catcatgtta ttgcccgaga
     5761 tttaagaatc cctcgcattt taatggccat cattgcaggt gctggtttat cacttgccgg
     5821 tttgctgatg caaactattt tttacaatcc tttggcagaa cctaatattt tgggtgtaag
     5881 cactggttca agtttgtttg ttgccattgc attaatgaca ggatttacgt tttttaattc
     5941 agattttgga ctcatttcaa gtgctctttt tggtgcgctt atttttggtg ttcttatctt
     6001 attttttgcc cgttttgtgc gctctcacgg ttctttattg ttgattggca ttatgctggg
     6061 tagtttctct gcagcattta ttagcattat tcaaacatgg agcgatgcac atcagttaaa
     6121 actctttacg ttgtgggcaa tgggttcact gcaacaagta acctatgcac aattgcccct
     6181 cattttcttg ctctttagtt caagtattgt tgccacattt ttcttagtaa aagcattgaa
     6241 cgcattggtt ttgggacaag aaaatgcagc tgttttagga gtgaatagtc gcaatacacg
     6301 catgttggtt attttaattg gctcggtttt aacaggtgtc attacagctt tttgtggccc
     6361 cattgcattt gttggaatgg cagttcctaa tttggtgaag ttgatataca aaacacaaaa
     6421 ccatcgaata ttaattgtag caacactagt gacaggaagt atttttatgg tag
//
LOCUS       DAOQVQ010000088         6468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13178_length_6468_cov_4.150164, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000088 DAOQVQ010000000
VERSION     DAOQVQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13178_length_6468_cov_4.150164"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKN22_04050"
     CDS             <1..519
                     /locus_tag="PKN22_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41907.1"
                     /translation="ETNEQLMQLKEELHSYFKLLKKVKLDEKYLYEYKTKNGSKAKEL
                     LMMVLLAPTAVLGLIHCGIPYFFIKRFVEKSFKRRVFWGSVKLLMGKIIMGLINIPVI
                     FLFYHFVYPSYWLGFVYYCLIGLFGLSAYMWFRNYKSYMIKSKMNDKDANKFWEKREA
                     LAEKIKILIPIA"
     gene            609..1247
                     /locus_tag="PKN22_04055"
     CDS             609..1247
                     /locus_tag="PKN22_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS41908.1"
                     /translation="MLENFSLDELKRQFKTNKTLKLTTYAVGGVLVLVIGYLAYYQFV
                     VTPKNKKSEDAYWYGLNLAANDSTDLAIEELTAQVKKYDGYKGGENAQFVLGRQYMEK
                     GDFKKALEVLKGVELEDNYLAAMAVGLQGDCYSEMKNYKEAAAKYNEAAKVSDNEWTS
                     PMYLFKAGLCAEELQDFDAALKLYKEIQDKYTIFANNKSIDKYIARVSNLKE"
     gene            1259..1750
                     /gene="ribH"
                     /locus_tag="PKN22_04060"
     CDS             1259..1750
                     /gene="ribH"
                     /locus_tag="PKN22_04060"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNS41909.1"
                     /translation="MATANRNLSEYNKEFVPNGADYKIGIVVSEWNETITLKLLEGAK
                     KALLDNGVLEENIEVRFVPGTFELSLGAQFFCEMTNVDAIIAIGVVIQGETRHFDFVC
                     QGATQGIMDTMLKYNKPIGYCVLTDENMQQSIDRAGGKYGNKGVDTAIAMMKMVELYR
                     SFR"
     gene            1763..3145
                     /gene="glmM"
                     /locus_tag="PKN22_04065"
     CDS             1763..3145
                     /gene="glmM"
                     /locus_tag="PKN22_04065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS41910.1"
                     /translation="MTLIKSISGIRGTIGGNVGDALTPVDAVRFAAAYGTWLKENHSG
                     KIKVVIGRDARLSGQMVSDLVVSTLVGLGIDVVNIGLSTTPTVEVAVPMENAQGGIIL
                     TASHNPKQWNALKLLNQKGEFISAEEGQRVLDIAEKDAYEFAQVDDLGVVTQDDSYMQ
                     KHIDAVLNLKLVDKKAIEAANFSIVVDAVNSTGGVFVPALLEALGVKNITKLYCEPTG
                     HFPHNPEPLPEHLTDLSKKIVEVKADLGITVDPDVDRLAMVCEDGTMFGEEYTLVAVA
                     DYVLTHTPGSTVSNLSSTRALRDVTEDRGLTYTAAAVGEVNVVQTMKATNAIIGGEGN
                     GGVIYPELHYGRDALVGIALFLSHLAHKKMSTSQLRDSYPKYTISKNKIELTPSINVD
                     AILASMALKYAAEEVDTTDGVKIYVGKEWVHLRKSNTEPIIRIYSESENEEKANQFAE
                     KIIAEIKALI"
     gene            3182..3943
                     /locus_tag="PKN22_04070"
     CDS             3182..3943
                     /locus_tag="PKN22_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS41911.1"
                     /translation="MKLHYRELGEGKPFVILHGLFGSSDNWQTHGKKLSEYYRVVMVD
                     LRNHGHSAWSDDFSYQLMVQDVKELFDDLNLQDAILLGHSMGGKVAMSFAQNYSEYLS
                     KLIVVDMGVKAYPPHHQQIFEAITTLDLSKLSNRLEAENHLAKFIDSPSVRQFLMKNI
                     YWKEKGVLGWRMNIEVLHREIETILEALDNKECFVQTLFIRGELSNYILDEDFTEIES
                     IFPDSDIVTIENAGHWVHAEQQELFMDAVLGFCLR"
     gene            3944..5107
                     /locus_tag="PKN22_04075"
     CDS             3944..5107
                     /locus_tag="PKN22_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNS41912.1"
                     /translation="MKKIVIVGGGPAGLMTAHQLSKKKQFDIQIFDANKAVARKFLVA
                     GQGGFNITNSEKTESFIQKYNHSFVQNAVLQFPNDATINWLKEIGIETYVGSSGKIFP
                     VKGTKPVEVLNNWLKFLVHKGVQINTEHKLVDFHSQKAFFSTPNGESIVDYDVLIFAL
                     GGASWKVTGSTGEWTTLFQQKNIKIQPFQSSNAGIEINNWEDSWGGNVLKNTSISIGN
                     TSRFGEIELTKYGFEGAPIYALNAQVRAGAKFLKLDLKPSKSYDELLQFYKQYKGNKT
                     GFLKAIKLSKSAIDLIKKQLTKEQFTDSEILVQSIKELTFPIQALRPIDEAISTVGGV
                     DMNEITMNFELKNHPNHFCIGEMVDWDAPTGGYLLQACFAQGVICAQAIREKN"
     gene            complement(5104..5499)
                     /locus_tag="PKN22_04080"
     CDS             complement(5104..5499)
                     /locus_tag="PKN22_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNS41913.1"
                     /translation="MKLVLTLVTTFLLINIGLSQGNVEVIKDARIDNLIKKQGEIVPP
                     ATSVQIHGYRLQIFFDSNKSLVDEARMRFTKSYPKIETYITYSAPNYFLRVGDFRNSS
                     DADRLKAELGAEFPASFIVQEKVNLPRID"
     gene            5746..>6468
                     /locus_tag="PKN22_04085"
     CDS             5746..>6468
                     /locus_tag="PKN22_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS41914.1"
                     /translation="MEISNTQMFRKINNWCLGALAVMLVSGTFEAQAQPNGETLFKQC
                     ATCHSPFKDGTGPKLQGVREKWASGGAKEGSLYLWVKDFEAAAAADPYAAGVEKVKPG
                     AMMKFPALTNEEIDAIFDYVDAQVEAAPAAPAGDGTASADVEVVEQELPMGWIWLAFA
                     IVLTVVVVSLSSVRNSLTMISKAKNGEDIAETPSFLDDMKAWVFRNKKLTAFLALCVV
                     IGTVVVLFLALYTIDVKEGYHPS"
BASE COUNT         2222 a          944 c         1278 g         2024 t
ORIGIN      
        1 gaaaccaatg aacaattgat gcaactaaaa gaggaactac atagctattt taaattgttg
       61 aaaaaagtaa aattggacga aaaatatttg tacgaataca aaaccaaaaa cggttcaaag
      121 gcaaaagaat tgttgatgat ggtgttgctt gcacctactg ccgttcttgg attaattcat
      181 tgtggtattc cttatttctt tatcaaacga tttgtggaaa aatcattcaa acgaagagta
      241 ttttggggtt ctgtaaaatt gttgatggga aaaattatca tggggttaat aaacattcct
      301 gttatctttt tattttatca ttttgtttat ccaagttatt ggttaggttt tgtttattat
      361 tgcctcattg gtttgtttgg tctgtcagcc tacatgtggt ttagaaacta taaatcatac
      421 atgataaaaa gtaaaatgaa cgataaagac gctaataaat tctgggaaaa aagagaggct
      481 ttagccgaaa aaataaaaat tctcattcca attgcttaaa aattagaact aaatagaaga
      541 ttaattcctt gttttaagga tttgaataaa aaaaaaatta tctttgctca ctttaaaatg
      601 gaagtacaat gttagaaaat tttagcctag acgaattaaa aagacaattt aaaacaaata
      661 aaactttaaa acttactacg tatgctgtag gaggcgtttt ggttttagtt attgggtatt
      721 tagcttatta tcaatttgtt gttactccaa aaaacaaaaa atctgaagac gcatattggt
      781 atggtttaaa ccttgcagct aacgattcta ctgatttagc aatagaagaa ttaactgctc
      841 aagttaaaaa atacgacggt tataaaggtg gtgaaaacgc acaatttgtt cttggacgtc
      901 aatacatgga aaaaggcgat ttcaaaaaag ctttggaagt attgaaaggt gtagagttgg
      961 aagataatta tttggcagct atggcagttg gacttcaagg tgattgctac agcgaaatga
     1021 aaaattacaa agaagctgct gcaaaataca acgaagcagc aaaagtaagt gataacgagt
     1081 ggacttcacc aatgtatttg ttcaaagcag gtttatgtgc ggaagaatta caagattttg
     1141 atgctgcatt aaagttgtat aaagaaatcc aagataagta cactattttt gcgaataata
     1201 aatcaattga taaatacatc gcaagagtat ctaatttgaa agaataaaaa attaaattat
     1261 ggcaacagcg aatcgcaatt tgtcagaata caataaggaa tttgttccaa atggtgccga
     1321 ttacaaaatc ggcattgttg tttctgaatg gaatgaaaca atcacattaa aattgctcga
     1381 aggtgctaaa aaagcgcttc ttgacaacgg tgtgcttgaa gaaaatattg aagtacgttt
     1441 tgttccaggt acttttgaat tgtcattagg agctcagttt ttttgtgaaa tgaccaatgt
     1501 agatgccatt atcgctattg gtgtggtgat tcaaggtgaa acacgtcatt tcgattttgt
     1561 ttgccaaggt gcaactcaag gtattatgga taccatgctt aaatacaata aacccatcgg
     1621 ttattgtgtg ttgacagatg aaaacatgca acaatccatc gatagagcag gtggaaaata
     1681 tggaaataaa ggtgtggata cagccattgc tatgatgaaa atggttgaat tgtatcgttc
     1741 ttttcgctaa aaacaaacta aaatgacctt aattaaatct atttcaggta tccgcggaac
     1801 aattggcgga aacgttggtg atgcacttac tcctgttgat gcagttcgtt ttgcggctgc
     1861 ttatggaact tggttaaaag aaaatcattc tggaaaaatt aaagttgtaa ttggaagaga
     1921 tgcacgctta tctggacaaa tggtgtcgga tttggtggtt tcaacgttgg ttggtcttgg
     1981 aattgatgtt gtaaacattg gtctttcaac cactccaact gttgaagtag ctgttccaat
     2041 ggaaaatgca caaggtggta tcatcttaac agcaagccac aatccaaaac aatggaatgc
     2101 gttaaagtta ttgaaccaaa aaggagagtt tatttctgcc gaagaaggtc aacgtgtact
     2161 tgatatcgct gaaaaagatg cttatgaatt tgctcaagtg gatgatttag gtgttgttac
     2221 acaagatgat tcctatatgc aaaaacacat tgatgctgtt ttaaacctta aactggtaga
     2281 taaaaaagct attgaagctg ccaatttctc aattgttgta gatgcagtta attcaactgg
     2341 tggagttttt gttccagcac ttttggaagc tttaggcgta aaaaatatta caaaattata
     2401 ttgtgaacca acaggacatt ttccacataa tccagagccg ttacctgaac atttaacaga
     2461 tttatctaaa aaaatagtag aagttaaggc tgatttagga attactgtcg accctgatgt
     2521 ggatcgttta gcaatggttt gcgaagacgg taccatgttt ggcgaagaat atactttggt
     2581 ggcagttgcc gattatgttt taacacatac acctggttca actgtttcca atttgtcatc
     2641 cacacgtgct ttacgcgatg tgactgaaga tcgtggttta acttataccg cagcagcagt
     2701 aggtgaagta aatgttgtgc aaaccatgaa agcaaccaat gccatcattg gtggagaagg
     2761 aaatggaggg gtaatttatc cagaattgca ctatgggcgt gatgctttgg ttggtattgc
     2821 cttattttta agtcatttgg cacacaaaaa aatgtcaaca tctcaattac gtgacagtta
     2881 tccaaaatat acaatttcca aaaacaaaat tgaattaaca ccttccatca atgtagatgc
     2941 aattttggct agtatggcat tgaaatatgc ggctgaagaa gttgatacaa ccgatggtgt
     3001 aaaaatatat gtaggaaaag aatgggtgca cttacgaaaa agcaatacag aacccattat
     3061 tcgtatctat tctgaaagcg aaaatgaaga aaaagcaaat caatttgctg aaaaaataat
     3121 tgctgaaatt aaagcgttga tttaatagtt ttacaatcat ttttcaataa aaaacgtcaa
     3181 attgaaactg cattacagag aattaggtga aggaaaaccg tttgtaattc tacacggttt
     3241 gtttggtagt tccgataatt ggcaaacaca tggtaaaaaa ttgagtgaat attaccgtgt
     3301 ggttatggtt gatttacgca atcatggtca ttctgcttgg agcgatgatt tttcctacca
     3361 attaatggtg caagatgtta aagaattgtt tgatgatttg aacttgcaag atgctatttt
     3421 attaggtcat tccatgggtg ggaaagtagc catgtcgttt gctcaaaatt attccgaata
     3481 cctctccaaa ttgatagttg tcgatatggg tgtaaaggcc tatcctccgc atcaccaaca
     3541 aatatttgaa gcaataacaa ccttggattt atccaaatta tccaaccgtt tagaagctga
     3601 aaaccatttg gcaaaattca ttgattcgcc ttctgttcgc caatttttga tgaaaaatat
     3661 ttattggaaa gagaaaggag tgttgggttg gcgtatgaat attgaagttt tacatcgcga
     3721 aattgaaact attctagaag cccttgataa taaagaatgt tttgtacaaa cactttttat
     3781 tcgtggcgaa ttgtccaatt atatcctcga cgaagatttt acagaaattg aaagcatttt
     3841 tccagattct gatattgtta caatagaaaa tgcgggtcat tgggtacatg cagagcaaca
     3901 agagttgttt atggatgccg tgctaggttt ttgtttgcga taaatgaaaa aaatcgtcat
     3961 agtggggggt ggtccagcag gtttgatgac tgcacatcaa ctttcaaaaa agaaacaatt
     4021 tgatattcaa atttttgatg ccaataaagc ggttgctcga aaatttttag tggcaggtca
     4081 aggtggattt aatatcacca attcagaaaa aaccgaaagt tttatccaaa aatacaacca
     4141 ttcctttgtt caaaatgcgg ttcttcaatt tccaaatgat gcaactatta attggttaaa
     4201 agaaattgga attgaaacct atgttggatc aagtggtaaa atatttccag taaaaggtac
     4261 caaaccagta gaagttttaa ataactggtt gaaatttctt gtacataaag gagttcaaat
     4321 caacaccgag cataaattag ttgatttcca ttctcaaaag gcattttttt ctactcctaa
     4381 tggtgaatcg atagttgatt acgatgtgct gatttttgca ttgggcggag catcttggaa
     4441 agtaacaggt tcaacaggag aatggacaac tctatttcaa caaaaaaaca ttaaaattca
     4501 accgtttcaa tccagtaatg ctggaattga aataaacaat tgggaggata gttggggagg
     4561 taatgtatta aaaaacactt ctatttccat tggaaacaca tcacgatttg gcgaaattga
     4621 acttacaaaa tacggctttg aaggtgcgcc aatttatgcc ttgaatgcac aagttagggc
     4681 aggagctaaa tttttaaaac tagacttaaa gccgagtaaa tcgtatgatg aattgttgca
     4741 attttataag caatataaag gtaataaaac aggattttta aaagccatta aattatccaa
     4801 aagtgctatt gatctaatta aaaaacaact caccaaagag caattcactg actcagaaat
     4861 tttagtgcaa tctataaaag aattaacttt tcctattcag gctttgcgac caatagatga
     4921 agcaatttca accgttggag gagttgatat gaatgagatt acaatgaatt ttgaattaaa
     4981 aaaccatcca aatcattttt gtattggtga aatggtagat tgggatgcgc ctactggtgg
     5041 atatttattg caagcatgtt ttgctcaagg ggtaatttgt gcgcaagcaa ttagagaaaa
     5101 aaattagtca attcgtggta aatttacttt ttcttgcaca ataaaacttg caggaaattc
     5161 agcacctaat tcagctttta aacgatcagc atcacttgaa tttctaaaat caccaactct
     5221 taagaaataa ttgggagcac tgtatgtaat gtaggtttca atcttcggat acgattttgt
     5281 aaaacgcatg cgcgcttcat caaccaacga tttatttgaa tcaaaaaata tttgaagtcg
     5341 atagccatgt atttgaaccg atgtggctgg aggcacaatt tcaccttgtt tttttatcaa
     5401 attatcaatt cttgcatctt taataacttc aacatttcct tgacttaaac caatgtttat
     5461 caataaaaaa gttgttacta aggttaaaac taatttcatg gcaatctatt tttaattaca
     5521 aaactaaaga atatttaatt tcttgcaaca atctttaaca acctttgtgc cgatttttaa
     5581 ttttataaca aatgttaaaa aagcacatca ggaatttatt tagaatcaat ataaattaat
     5641 ggttttagaa gaaaaaatgt cattaaactg tcataaaagt tggttaatat tctaaatttg
     5701 ctccgctagt agtgttatat ttaacatgat tattaacatt ttttaatgga aatatctaat
     5761 actcaaatgt ttagaaaaat taataattgg tgccttggtg cattagcggt gatgctagtg
     5821 tcgggtacgt ttgaagcgca agcacagcca aacggagaaa cgttgtttaa acaatgtgct
     5881 acatgtcaca gtcctttcaa agatggaaca ggaccaaaat tgcaaggagt tcgtgaaaaa
     5941 tgggcttcag gcggagcgaa agaaggttct ctttatcttt gggtaaaaga ttttgaggct
     6001 gccgctgcgg cagatcctta tgctgctggg gtagaaaaag tgaaaccagg ggcaatgatg
     6061 aagtttcctg cattgaccaa tgaggaaatt gatgcaattt ttgattacgt agatgcacaa
     6121 gtagaagcag ctccggcagc tccagcaggt gatggaactg caagtgcaga tgttgaagtg
     6181 gttgaacaag aattgccaat gggatggatt tggttggctt tcgcaattgt cttaacagtt
     6241 gtagtagttt cattaagtag tgttagaaat agcttaacta tgatttctaa ggctaagaat
     6301 ggagaagata ttgcagaaac tccatctttc ttagacgaca tgaaagcatg ggtgtttaga
     6361 aataaaaaat taacggcttt cttggcattg tgtgtagtga ttgggacggt tgtagtatta
     6421 tttttagcat tgtacactat cgacgtaaaa gaaggttatc atccttca
//
LOCUS       DAOQVQ010000089         6425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13297_length_6425_cov_4.249608, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000089 DAOQVQ010000000
VERSION     DAOQVQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13297_length_6425_cov_4.249608"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PKN22_04090"
     CDS             <1..767
                     /locus_tag="PKN22_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNS41915.1"
                     /translation="FAHSWMPGNPYNSPEKPQYTDQHNLLPTKMTDVNGARGNRPFGE
                     VITPNFTFMGTKRGVDANGNEVYEPRDEIKGDVARCLMYEAVTYNGTNGTWAFPSFIS
                     FIFPYGQNADVILQWHFQDPPSDLEIARNEYIYSIQGNRNPFIDSVDFACYIDFSTMN
                     YDADACGDLSVNSIVTPADVMVYPIPANETINIVSPKFEISAIELVDNQGRIVVANNN
                     IANTVAHINVNTVTSGIYLVNIHTTNGTITQRVVIQ"
     gene            complement(971..1855)
                     /locus_tag="PKN22_04095"
     CDS             complement(971..1855)
                     /locus_tag="PKN22_04095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS41916.1"
                     /translation="MKKIIAFVLPLAVIVACGGKDKEEVIKFDSEKQKLSYALGADFA
                     SPIVNAGAEASSILDFEAVSKGFEAALNDGDYKSCEETVIGAFGATFNTPDSTKKIPG
                     SECFGKLNGSRMYQMLKEFGKIDKFDMKYLAYGYRDALLKKDTILDKGERASLLTAFQ
                     TEIQTEQTKKMQELEKPFFDKAKALKNAKTIEGGIVIETVEEGKGGSPVVTDDVTAHY
                     ILTNTKGDTLETSFDRGQPLTISLQSVIPGWTMAFPQLKKGGKYRLYIPADLAYGNSK
                     GALCFYVELIDFKKGATK"
     gene            complement(1855..2451)
                     /locus_tag="PKN22_04100"
     CDS             complement(1855..2451)
                     /locus_tag="PKN22_04100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS41917.1"
                     /translation="MSEELDAVSYCVGMSIAGSLIQQNLGTLSVEAFTEALEDVLQGK
                     PTKYSPEQANAIIQQFMEKATQQQFEANKLAGEKFLSENFKKEGVKTTLSGLQYEVIK
                     EGEGNHPSVNSTVTVHYEGTLIDGTVFDSSIKRGTPATFGVSQVIPGWTEALQLMNKG
                     SKYRLYIPQELAYGAHAPGGSIQPYMALIFDVELIDFK"
     gene            2963..3544
                     /locus_tag="PKN22_04105"
     CDS             2963..3544
                     /locus_tag="PKN22_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS41918.1"
                     /translation="MIAPKEIKELVANYLSISDGLANELAAIATLVHYKKNDTIYVAN
                     EITSTVGFIKSGLVHSFVYDFNKHPKTVWFGEPGDFITSFHAFFTNNPGIETIQCIEP
                     TTVVQFESHAFKALTQKNPEMLLLYSKILEECCLFWENRYIINQINEAEERYADFYYR
                     AKEMIIRIPLKLLASYLNIRQETLSRIRKNWLK"
     gene            3685..3954
                     /gene="rpsO"
                     /locus_tag="PKN22_04110"
     CDS             3685..3954
                     /gene="rpsO"
                     /locus_tag="PKN22_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNS41919.1"
                     /translation="MYLSTEKKKEIFAKHGGSETNTGSTEGQIALFTYRIAHLTEHLK
                     NNKKDYNTQRSLQLLVGKRRSFLDYLKRKDIERYRAIVKELGLRR"
     gene            4049..6178
                     /locus_tag="PKN22_04115"
     CDS             4049..6178
                     /locus_tag="PKN22_04115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNS41920.1"
                     /translation="MNIGIKKSIITGGKEISIETGKLAKQADGSVVLRMGDTMILATV
                     VANKEAGEGVDFLPLTVDYKEKFAAAGRIPGGFFRREARPSEREILVMRIVDRALRPL
                     FPDDYHAEVQVMMQLIAYDGINSPDALVGFAASTAIAVSDIPFNGPMSEVRIGRVDGQ
                     FIINPTLEEMKVSDIDMIIAGTAKDINMVEGEMHEISEDEMIEAIKLAHEEIKKHCQF
                     QLDLAAEIPTANPKREYCHEKHDEEFRQKLYDFAFEKCCDIARQGLASKEKRAELFGA
                     VKDEFFAQFSEEDQEKYKPFLGGYFKETMKKAVRTVMLDEGKRLDGRKFNEIRPIWSE
                     VDYLPIVHGSSIFTRGETQSLTTLTLGGKQDEQMIDDVTYEGTESFMLHYNFPPFSTG
                     EARPVRGTSRREIGHGNLALRALKRVLPADFPYTIRLVSEILESNGSSSMATVCAGTL
                     ALMDGGVPITAPVSGIAMGLVADGGKFAVLSDILGDEDHLGDMDFKVCGTAKGITACQ
                     MDIKVDGLPYEILAQALNQAKEGRLHILGKITETIAVPNPELKPQTPRIEAFNVPNDV
                     IGAIIGPGGKIIQKIQKDTNTTISIEELETGEGKVQILSNNADDMKAAVKAVRLIAFP
                     PQVEEGEEYEGKVKGIKEFGCFVEILPGTDGLIHISEFSWEKVAKMEDVCKEGDVLKF
                     KVIGKDPKTKKWKLSRKVLLPKPEAAN"
     gene            6246..>6425
                     /locus_tag="PKN22_04120"
     CDS             6246..>6425
                     /locus_tag="PKN22_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41921.1"
                     /translation="MANRRQIKKVINNVIIDIVEASYSVQLFNESKTEASNKIIDAAA
                     ELQDDLLSRINNVKSK"
BASE COUNT         2173 a         1074 c         1164 g         2014 t
ORIGIN      
        1 tatttgcaca ttcttggatg cctgggaatc catacaatag tccagaaaaa cctcaataca
       61 ccgaccaaca caatttatta cctacaaaaa tgaccgatgt aaacggagca cgcggtaatc
      121 gtccatttgg tgaggttatt acacctaatt ttacattcat gggcacaaaa agaggggttg
      181 atgcaaatgg taatgaagtg tatgaaccaa gagatgaaat taaaggagat gtagcgcgtt
      241 gtttaatgta tgaagcagtt acttacaacg gaacaaatgg aacatgggct ttcccaagct
      301 ttattagctt catttttcct tatggacaaa atgcagatgt gattttacaa tggcacttcc
      361 aagacccacc gtctgattta gaaattgcta gaaacgaata catttacagt attcaaggaa
      421 acagaaatcc atttattgac tcagttgact ttgcttgtta cattgatttt tcaacaatga
      481 actatgatgc agatgcatgt ggtgatttaa gtgttaattc tattgtaaca ccagcagacg
      541 ttatggttta tccgattcca gctaatgaaa ccataaacat tgtatctcca aaatttgaaa
      601 tttctgctat agaattggta gacaatcaag gaagaattgt agtagcaaat aacaacattg
      661 cgaatacggt tgctcatata aacgttaaca cggttacaag tggaatttac ttagtaaata
      721 tccatacaac caacggaaca attacacaac gagtagttat tcaataatag ttttattttt
      781 aaaagagcgt gttagtcttt ggattgacac gctttttttg ttacttcata aaagtgttag
      841 atggctaatt tcttatttta tttacatcga aaaatagtaa acaaacatag attttaaagc
      901 ttatttaaag gcacaaaaaa aggtcaactg cattaacaat tgacctttaa aaattgtata
      961 atttgtacta ttatttagta gctccttttt taaagtcgat taattcaaca taaaaacaca
     1021 aagctccttt gctgtttcca taagctaaat cagctggaat gtacaaacgg tattttcctc
     1081 cttttttcaa ttgtggaaat gccattgtcc aacctggaat taccgattgt aaactaattg
     1141 ttaatggttg ccctctatca aacgatgttt ctaaggtatc cccttttgta ttggttaaaa
     1201 tataatgtgc agtaacatca tctgtaacaa ctggtgaacc acctttaccc tcttcaaccg
     1261 tttcaataac aattcctcct tcaattgttt tagcattttt caaagctttt gctttatcaa
     1321 agaaaggttt ttccaattct tgcatttttt tggtttgctc agtttgtatt tccgtttgaa
     1381 aagctgtcaa taaagatgca cgttcacctt tatccaagat agtatctttt ttcaacaacg
     1441 catcacggta tccgtatgcc aagtacttca tatcgaattt atcgatttta ccaaactctt
     1501 tcaacatttg atacattctt gatccgttca atttaccaaa acattcagat ccgggtattt
     1561 ttttagtaga atctggcgta ttaaacgttg ctccaaatgc tccaattact gtttcttcgc
     1621 agcttttata atcaccatcg ttcaatgccg cttcaaaacc tttagaaaca gcttcaaaat
     1681 ccaaaatact tgaagcttca gcaccagcat ttacaattgg gctcgcaaaa tcggcaccca
     1741 aagcatagga caatttttgt ttctcagaat caaacttgat tacttcttct ttatcttttc
     1801 cgccacacgc aacaattact gctaatggca aaacaaatgc aattattttt ttcattattt
     1861 aaaatcaatt aattcaacat caaaaattaa tgccatatat ggctgaattg aaccacctgg
     1921 agcatgtgcc ccataagcca gttcttgtgg tatatataaa cggtattttg aacctttgtt
     1981 cattaattgc aaagcttctg tccatcctgg aattacttga cttacaccaa acgttgccgg
     2041 tgttcctctt tttatagaac tatcgaaaac agttccgtca atcaaggtgc cttcatagtg
     2101 cactgtaaca gttgaattga cagatggatg atttccctcc ccttctttta ttacttcgta
     2161 ttgtaaacct gaaagtgttg ttttaacccc ttctttttta aagttttctg ataaaaattt
     2221 ctcacctgct aacttgtttg cctcaaattg ttgttgagta gctttttcca taaactgctg
     2281 gataattgca ttggcttgct ctggagaata ttttgttggt ttgccttgta atacatcttc
     2341 caatgcttct gtaaaggctt caacagataa agttcctaaa ttttgttgga ttaaacttcc
     2401 tgcaatgctc atgcctacac aatagcttac agcatctaat tcttcgctca tatacttgat
     2461 taattttaag gtacaaatgt aatgactttt catggaagat aaagtgcaca aaaaattatt
     2521 atttaaaaga atgtagcgat tagtaataaa aaaaccgatg cacacttggc acatatacag
     2581 tgaacttcac aacaaaaggt atggcaataa gtaaaaaaac aactgctttc atcattttct
     2641 agtttttatt agtaaaatat aggtagcacg taaagcagtt gttgaaaaat gaacaatggt
     2701 agtgcgtatt agtaaattga atgaagtaca ctacagcaat tatgctgaat gcttaaatag
     2761 aacgtaatgg atgttttcgt tctgttagct aaaactggtg agcgtgtgag tataaaacta
     2821 ctgtttgcaa cagtagcgct gttttgtcga agaaaaaaat cattcgtcga aaaattaaac
     2881 taattgatat aaatcaaata aaaagaagaa agtattcgtg tatttatata ttttacatta
     2941 catttacctg actgttaaac aaatgattgc tccaaaagaa ataaaagaat tagttgcaaa
     3001 ctatttatcc atcagtgatg ggttggcaaa tgaattggct gcaattgcta cgttggtgca
     3061 ctataaaaaa aatgacacta tttatgttgc caatgaaatc acaagtactg ttggttttat
     3121 caaaagtggt ttggtacatt cgtttgtgta cgatttcaat aaacacccca aaactgtttg
     3181 gtttggtgag ccaggtgatt ttataacgtc ttttcatgct ttttttacca ataacccagg
     3241 aatagaaacc attcagtgta tagaaccaac aactgtagtt caatttgaaa gtcatgcgtt
     3301 taaggcttta actcaaaaaa atcctgaaat gttattgctg tattctaaaa tattggaaga
     3361 atgttgtttg ttttgggaaa accgttacat tattaatcaa atcaatgaag ccgaggagcg
     3421 ttatgccgat ttttactaca gggctaaaga aatgataatt cggattccat taaaattatt
     3481 ggcaagttat cttaatattc gtcaggaaac gctgagtaga attagaaaaa attggttgaa
     3541 ataaaaatct attcctttta aataccaact ccctttttaa agtatatttc aaaaaattaa
     3601 gttaaaaata gtctactaca ataattctac ttatatttgc acacccaaag tgggttacat
     3661 aataatcatt taaacaatat ttgcatgtat ttaagtactg aaaagaagaa agaaatcttc
     3721 gcaaaacacg gtggttctga aacaaataca ggatctacag aaggacaaat cgcattgttt
     3781 acatacagaa ttgcgcattt gactgaacac ttgaaaaata acaaaaaaga ttacaacact
     3841 caacgttcat tacagttgtt ggttggtaaa agaagaagct ttttggatta tttgaaaaga
     3901 aaagacatcg aaagatatcg tgctatcgtt aaagaattag gattacgtcg ttaattcttt
     3961 acgaaagtaa atttattgta gaggggacat cgattttggt cgaatgtccc ttttatgttt
     4021 tttatacaac ttaagtaaat aattaaatat gaatatagga atcaaaaagt ccatcattac
     4081 cggaggtaaa gaaatttcca ttgaaacagg gaaattggca aaacaagccg atggttcggt
     4141 agtgttacga atgggcgaca caatgatttt agcgactgtt gttgctaata aagaagcagg
     4201 agaaggcgtt gactttcttc cactaactgt tgactacaaa gaaaaatttg ctgctgcagg
     4261 tcgaattcca ggtggtttct tccgccgtga agcgcgtcca tcagagcgtg aaattttagt
     4321 aatgcgcatt gttgaccgtg cgttacgtcc acttttccca gatgattacc atgctgaggt
     4381 gcaagtaatg atgcaattaa ttgcttatga tggcatcaac tcaccggatg cattggtagg
     4441 ttttgctgca tctacagcaa ttgctgtttc agatattcca ttcaacggac caatgtcaga
     4501 agtgcgtatc ggtcgtgtag atggtcaatt cattatcaac cctacgttgg aagaaatgaa
     4561 agtatcagac atcgatatga ttattgctgg tacggcaaaa gacatcaaca tggtggaagg
     4621 tgaaatgcac gaaatttctg aagacgaaat gattgaggca atcaaactag cgcacgaaga
     4681 aattaaaaaa cactgtcaat tccaattgga tttagcggca gaaattccaa ctgctaatcc
     4741 aaaacgtgaa tattgccatg aaaaacacga cgaagaattc cgtcaaaaat tatacgattt
     4801 tgcatttgaa aaatgttgcg acattgctcg tcaaggactt gcatctaaag aaaaacgtgc
     4861 agaattattt ggcgcagtaa aagacgagtt ctttgctcag ttttcagaag aagatcaaga
     4921 aaaatacaaa ccattcttgg gcggttattt caaagaaaca atgaaaaaag ctgttcgtac
     4981 agttatgttg gatgaaggaa aacgtttgga tggtcgtaaa tttaacgaaa tcagacctat
     5041 ttggtcagaa gttgattact taccaattgt acacggttct tctattttta cacgtggtga
     5101 aacacaatcg ttgacaacct taacattggg aggtaaacaa gacgaacaaa tgattgatga
     5161 cgtgacttat gaaggaacag aatcgttcat gttgcactac aacttcccac ctttttctac
     5221 aggtgaagct cgcccagtta gagggacttc tagaagagaa attggtcacg gaaacttggc
     5281 attaagagcc ttgaaacgtg ttttaccagc agatttccct tataccattc gtttggtttc
     5341 tgaaatttta gaatccaatg gttcatcatc tatggcaact gtttgtgcag gtactttagc
     5401 attgatggac ggaggtgttc cgatcacagc accagtttct ggtattgcaa tgggattggt
     5461 tgcagatggt ggaaagtttg ctgttttgtc tgatatttta ggagatgaag accatttggg
     5521 tgacatggac tttaaagttt gtggtacagc aaaaggaatt actgcttgtc agatggatat
     5581 taaagttgat ggtttgccgt acgaaatttt agcacaagca ttgaatcaag caaaagaagg
     5641 tcgtttacac attttaggta aaatcaccga aacaattgca gttccaaatc ctgagttgaa
     5701 accacaaaca ccgcgtattg aagcgttcaa cgttccaaac gatgtgattg gtgcaattat
     5761 cgggcctgga ggaaaaatca tccaaaagat tcaaaaagat accaatacaa ctatttcaat
     5821 tgaagagttg gaaacaggcg aaggtaaagt tcaaatcttg tcaaacaatg ccgacgatat
     5881 gaaggcagct gttaaagcgg tgcgtttaat tgctttccca cctcaagttg aagaaggtga
     5941 agaatacgaa ggaaaagtaa aaggcatcaa agaatttgga tgttttgttg aaattttgcc
     6001 tggaacagac ggtttaatcc acatttctga gttttcttgg gaaaaagtag caaaaatgga
     6061 agacgtttgt aaagaaggtg atgttttgaa atttaaagtt attggaaaag atccaaaaac
     6121 taaaaaatgg aaattatcac gcaaagtttt gttaccaaaa ccagaagcag caaattaata
     6181 gaagtttaaa ataatttcgt acctttgaag tccgttcata agagcggact ttttaattaa
     6241 aaattatggc aaatagaaga caaatcaaaa aggtaatcaa taacgtaatt attgatatcg
     6301 ttgaagctag ttattcagtt caattgttta atgaaagcaa aacagaggca agtaacaaaa
     6361 ttattgatgc agctgcagag ttgcaagatg acttgttgag cagaattaac aatgttaaat
     6421 ctaaa
//
LOCUS       DAOQVQ010000090         6353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13504_length_6353_cov_4.322483, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000090 DAOQVQ010000000
VERSION     DAOQVQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13504_length_6353_cov_4.322483"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKN22_04125"
     CDS             complement(<1..634)
                     /locus_tag="PKN22_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HNS41922.1"
                     /translation="MNIDEKQLLHHWIHQQSEFNKNDLLVAVDPRKSSVFQPTAPLSD
                     REKTIALAASTIKESGVNPLCTAKGLLTWEFNKQIIQTPLWLLPTQFEVDKVRAQVQF
                     FADDENGFVNPFLRKKMEELYAICLDETSFETVVTTLQNAGFQQVDAAFSTLGNFHHH
                     RYVLLKELQDVASCSSYSSPLQQLLSGEKNNRKTIQFSHQALLPYDTDHRM"
     gene            complement(714..1031)
                     /gene="trxA"
                     /locus_tag="PKN22_04130"
     CDS             complement(714..1031)
                     /gene="trxA"
                     /locus_tag="PKN22_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNS41923.1"
                     /translation="MALEITDANFEEVVLKSSVPVVVDFWAEWCGPCRMIGPVIEEMA
                     GEYEGKAVIGKVNVDLNPGVSAQFGIRNIPTVLFVKNGEIADKVVGAVPKQQLTSKLD
                     AIL"
     gene            complement(1139..1531)
                     /locus_tag="PKN22_04135"
     CDS             complement(1139..1531)
                     /locus_tag="PKN22_04135"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41924.1"
                     /translation="MILAFTAMEVVVGTMIFTLALLLLVIAYKKFLAYLGKGAIPKEK
                     YCVLYSLEANPASGKVQFYFTSEEEKNVKFHILDNDYNFVKELYKQQCSTDGNIIHFD
                     TAELQNGVYYYCLLTENQKTMKRMTVHN"
     gene            complement(1528..1797)
                     /locus_tag="PKN22_04140"
     CDS             complement(1528..1797)
                     /locus_tag="PKN22_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HNS41925.1"
                     /translation="MAMEVFDKLEKQLEQEEWPQVYFFKFIVPNTSEHIAQVFALFEE
                     EANITSQTSRNGKYTSISAKILMLDAASVIEKYKEASKINGLIAL"
     gene            complement(1778..2368)
                     /locus_tag="PKN22_04145"
     CDS             complement(1778..2368)
                     /locus_tag="PKN22_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41926.1"
                     /translation="MTAEEAKIFFPHTEDDDLQDLYDERFFEYKNFFLSKIPIKKVVD
                     AKINRLLQMENAFRFLQNEEAVSIVVSTNPAIIEFSDNVLQAFNQWEQQKGMCKQRIV
                     AASTIETLVEAVNYYVQVTQAYWQKWAIAPDLEVEVEQLSKEEDPMQLYAAIQQFNAA
                     GGVTFEDILTVNNNLFLLKEMKRLSLLAQNYGNGSI"
     gene            complement(2365..3615)
                     /gene="tilS"
                     /locus_tag="PKN22_04150"
     CDS             complement(2365..3615)
                     /gene="tilS"
                     /locus_tag="PKN22_04150"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNS41927.1"
                     /translation="MHFNNQPVWHEVLAFLEQYRQQTIFVGCSGGVDSMVLVHFLLHN
                     DFQMHVVHVNYHKRGEESDKDMALVQQFCSNNNIPHTLADYQSTKKGNFQELARQFRY
                     DLLQQLAQENGMIALAHHFDDQVETFFMNLMRQSGIVGLAAMPRMRHQFIRPFLHLNK
                     QDVLDYAHQNKVEWREDASNQTTNYLRNKWRLEFLPLIEKTQPNIKQAVKIIVGAFQQ
                     TQQELQQKMEPIAQKIQQTQQLSKAQMEHFSTEELFELWRQLQQPAQLFPRFSEIQSL
                     QKGKHIELHAPYTKLVNEGTYFSFISNKRVALPTLDIQMVKELPQHFSKTTIYLDATK
                     IVGQLQLRKWKQGDKISPVGMKGEQLVSKIMKDAKISHALRENTWVVCDEQHIHWVVG
                     LKVGKFALAQSNSTDILAVKLAEK"
     gene            complement(3602..4801)
                     /locus_tag="PKN22_04155"
     CDS             complement(3602..4801)
                     /locus_tag="PKN22_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNS41928.1"
                     /translation="MNNQSIAYFNSNSKGANYLCWGQKDAVVLNNPIDFQALQNFIDA
                     HKKQFIGGFLSYDVKNSIENLTSNNFDGLHFPDCVFFVPSNVVEISANGMQFIQGDDT
                     MENRQQAEELLSQLSTKSSNIKFNFQPRISKQQYIEHVNQLKAHIQRGDIYEINYCQE
                     FYDANAQIDEPLNLYATINEITQAPFSAFFQWQEFSAFCGSPERFLQKNGNQLLSQPI
                     KGTAPRGKTSEEDQQLMLELKNNPKERSENVMIVDLVRNDLSKIATQGSVKVNELFGV
                     YTFNTVHQLISTISCQIDESCDFSTIIKATFPMGSMTGAPKVSAMKLSEEHEAFQRGL
                     YSGTIGCILPNGNFDFNVVIRSLLYNANNKYLSCGVGGAITINSDAEKEYQECETKVR
                     RLLNAFQ"
     gene            complement(4835..5605)
                     /locus_tag="PKN22_04160"
     CDS             complement(4835..5605)
                     /locus_tag="PKN22_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41929.1"
                     /translation="MKIISVVLSVFVVCFTFAQINQTDAKGKKQGQWVKLYPNSKVAM
                     YQGVFKNDKPIGTFTYRYPSNKVKAVIKHSETTPRSEAFFYYENGKLMTHGIYRNMKK
                     DSVWTSFNEGGRITMTETYKNDVLNGMKKMYYLPSDPEDLQETVISEYNYVDGKPEGK
                     FAEYYPTKPTRTLKLTGQYKNHKRTGEWVGYELSGQKMYVEHYLDGKMHGWFIGYNKE
                     DGKEGQRRYYHFGRLVEGEQLKALMKQMKEKGINPNGQ"
     gene            complement(5592..6143)
                     /locus_tag="PKN22_04165"
     CDS             complement(5592..6143)
                     /locus_tag="PKN22_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNS41930.1"
                     /translation="MVNWNIDASWTLFLDRDGVINHRKMGGYIETIEEFQFLPNVKEA
                     IAGLSQHFQHIFVVTNQQGIGKGIMTESNLQVIHRYMVNEVEQAGGKISNCYHAPALA
                     KENSNLRKPNIGMGLLAKKDFPEVDFSKAIMVGDSDSDIQFGMNLGMKTVRIVGNDLP
                     KIQADVTVDSLFEFLKLVENENH"
     gene            complement(6146..>6353)
                     /locus_tag="PKN22_04170"
     CDS             complement(6146..>6353)
                     /locus_tag="PKN22_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS41931.1"
                     /translation="IGFGFSFYLAVDKLFFNTHAIKIADRTEFYIALVAMIIGVQFFL
                     AGFIAELIGRNSSTRNTYLIEKEL"
BASE COUNT         1824 a         1238 c         1091 g         2200 t
ORIGIN      
        1 ccatccgatg atcggtatca tagggcaaca acgcttggtg tgaaaattga attgtttttc
       61 tattgttttt ttcacctgaa agtaattgct gtaagggcga agaataactg gagcacgaag
      121 ccacatcttg caattcttta agcaaaacat agcggtgatg atgaaaatta cctagggtag
      181 aaaacgctgc atccacttgt tggaaaccag cattttgaag tgtagtaacc accgtttcaa
      241 acgatgtttc gtccagacaa atagcgtaca attcctccat tttttttctt aaaaacggat
      301 tgacaaaacc attttcatcg tcagcaaaaa actgtacttg tgcgcgcact ttgtccacct
      361 caaattgtgt tggcaacaac caaagtggcg tttgaataat ttgtttatta aactcccaag
      421 ttaataaccc cttagcggta cacagtggat taacaccact ttctttgatg gttgaggcag
      481 ctaaagcaat tgttttttcc cgatcactta aaggtgctgt gggttgaaat accgatgatt
      541 ttcgcgggtc aactgccacc aacaaatcat ttttattgaa ctcactttgt tggtgaatcc
      601 aatggtgcaa caattgtttt tcatcgatat tcattatgct aaaatagtat aaaattttaa
      661 aaacaaaaaa accgatgatt gctcatcggt tgttctattt tgtggagtaa ttattacaaa
      721 attgcgtcta attttgaagt caattgttgt tttggaactg caccaactac tttgtcagca
      781 atttctccgt ttttaacgaa aagtacagta ggaatgttgc gaattccaaa ttgagcagaa
      841 acacctggat ttaagtcaac gttaacttta ccaataactg cttttccttc gtattcacct
      901 gccatttctt caataactgg tccaatcatt ctacatggtc cacaccattc tgcccaaaag
      961 tcaactacta ctggaacaga agatttcaat acaacttctt caaagtttgc gtctgtgatt
     1021 tctaatgcca taattatcta ttttttaaat ttatattctt atctaacgta ataaatgtta
     1081 ggttctttac ttaatcaata aacttgccaa tgcatttttt gtgtcaatat gacagcattt
     1141 aattgtgcac tgtcatgcgt ttcattgttt tctgattttc tgtcaacaaa caataataat
     1201 aaacgccatt ttgtagctcg gcagtatcaa aatgaatgat gttgccatcg gtgctacatt
     1261 gttgtttgta caattctttt acaaaattat aatcgttgtc taaaatatga aattttacat
     1321 ttttttcttc ttcggatgta aaataaaatt gcacttttcc agaagctgga ttcgcttcca
     1381 aactatataa aacacagtat ttttcttttg gaatggcgcc ttttcctaaa taagccaaga
     1441 actttttgta ggcaataacc aaaagtaaaa gtgcgagggt aaaaatcatc gttcctacca
     1501 ctacttccat tgctgtaaat gctaaaatca tagagctatt aatccgttaa tttttgatgc
     1561 ttctttgtat ttttcaataa ctgatgcggc gtctagcatc aatatttttg cagaaatact
     1621 tgtgtatttt ccatttctgg aagtttgtga agtaatatta gcttcttctt caaacaacgc
     1681 aaaaacttgt gcaatgtgct ctgatgtatt gggcacaatg aacttgaaaa aatagacttg
     1741 aggccattct tcttgctcca attgtttttc taatttatca aatacttcca ttgccataat
     1801 tttgagctaa caaagataag cgtttcattt cttttaagag aaaaagattg ttatttactg
     1861 ttaaaatatc ttcaaaagtg acaccgccag ctgcgttaaa ttgctgaatg gccgcataca
     1921 attgcatagg gtcttcttct ttggataatt gctcaacttc aacttctaaa tctggtgcaa
     1981 ttgcccattt ttgccaatag gcttgagtaa cttgcacata ataatttacg gcctctacca
     2041 atgtttcaat ggtgcttgcc gctacaatgc gttgtttaca cattcctttt tgttgctccc
     2101 attgattaaa cgcctgcaaa acattatctg aaaactcaat aattgctgga tttgttgaaa
     2161 ctacaatcga cacagcttct tcattctgta aaaaccgaaa ggcattttcc atttgcaaca
     2221 aacgattgat tttggcatct accacttttt tgatgggaat tttggataaa aagaagtttt
     2281 tgtactcaaa aaaacgctca tcgtacaagt cttgcaagtc atcgtcttct gtatgtggga
     2341 aaaatatttt tgcttcttct gctgtcattt ctctgccaat tttactgcta aaatatcggt
     2401 gctattggat tgcgccagtg caaatttacc aacttttaat ccaaccaccc aatgaatgtg
     2461 ttgttcatca caaactaccc acgtattttc tcgtagtgca tgcgaaattt ttgcatcttt
     2521 cataatttta gataccaatt gctcaccttt catcccaaca ggcgaaattt tatcgccttg
     2581 tttccatttg cgcaattgca actgaccaac aatttttgtt gcatctaaat aaatcgttgt
     2641 ttttgaaaaa tgttggggca gttcttttac catttgaata tccaacgtag gcaacgcaac
     2701 tcgcttgttt gaaatgaatg agaaatacgt tccttcattt accaattttg tataaggcgc
     2761 atgcaattca atgtgttttc ctttttgcaa tgattggatt tcagaaaatc gaggaaacaa
     2821 ctgtgcgggc tgctgcaatt gtcgccacaa ttcaaacagt tcttcggtag aaaaatgctc
     2881 catttgtgct ttgctcaatt gttgagtttg ttgaattttc tgggctattg gctccatttt
     2941 ttgttgcaac tcttgttgcg tttgctgaaa agcaccaaca ataattttca ccgcttgttt
     3001 aatattcggt tgtgtttttt ctattagtgg caaaaactcc aatcgccatt tattgcgcaa
     3061 atagttggtt gtttgattgg aagcatcttc tcgccattct accttatttt ggtgtgcata
     3121 atccaacaca tcttgtttat tgaggtgcaa aaaaggacga ataaattgat ggcgcatacg
     3181 gggcatggct gccaatccta caattcccga ctgacgcatc aggttcataa aaaacgtttc
     3241 tacttggtcg tcaaaatggt gtgccaaggc tatcattcca ttctcttgag ccaattgttg
     3301 caataaatcg tagcgaaatt gacgtgccag ctcttgaaaa tttccttttt tagtggattg
     3361 gtaatcggct aatgtatgcg gaatgttgtt gttgctacaa aattgttgca ccaatgccat
     3421 gtctttatcg ctttcctcgc ctcgtttgtg gtagttaaca tgcaccacat gcatttgaaa
     3481 atcgttgtgc aacaaaaaat gaacaagcac catagaatcc acaccaccac tacaaccaac
     3541 aaaaattgtt tgttgacggt attgttctaa aaaggccaat acttcgtgcc acacaggttg
     3601 attattgaaa tgcattgagt aaccttctta cttttgtttc acactcttgg tattcttttt
     3661 cagcatcgct gttgatggta atggcacctc caacgccaca cgataaatat ttgttgttgg
     3721 cattgtatag caaactgcga ataacgacat taaaatcaaa attaccgttg ggcaagatgc
     3781 agccaatggt tccggaatac aatccacgtt gaaatgcttc gtgttcttca ctcaatttca
     3841 tagcactcac tttgggtgct ccggtcatgc ttcccatagg aaaagtagct ttgatgatgg
     3901 ttgaaaaatc acacgattca tcaatttggc agctgatggt tgaaatcaat tggtgcaccg
     3961 tattgaacgt atagacacca aacaattcat tcactttgac actgccttgg gttgcaattt
     4021 ttgacaaatc gttgcgcacc aaatcaacaa tcattacatt ttctgagcgt tctttgggat
     4081 tgttttttaa ttccagcatc aattgttggt cttcttcact cgtttttcct cttggagcag
     4141 ttcctttgat gggctgtgaa agcaattgat tgccattttt ttgtaaaaat cgttcggggc
     4201 taccacaaaa tgctgaaaat tcttgccatt gaaaaaatgc tgaaaaagga gcttgcgtaa
     4261 tttcattgat ggtagcatac aagttcaacg gctcatcaat ttgcgcattg gcgtcataaa
     4321 attcttggca ataattgatt tcgtaaatat cgcctcgttg aatgtgtgct tttagttggt
     4381 tgacgtgttc aatgtactgc tgttttgaaa ttcgcggttg aaagttaaat tttatgttgg
     4441 atgattttgt tgaaagttgg ctcaacaatt cttctgcttg ttgacggttt tccattgtgt
     4501 catctccttg aataaattgc atgccattgg ctgaaatttc aacaacattg gagggcacaa
     4561 aaaacacaca atctgggaaa tgaagtccat caaaattgtt ggaagttaaa ttttcgattg
     4621 aatttttaac atcgtagctc aaaaaaccac caataaattg ttttttatgg gcgtcaataa
     4681 aattttgtag cgcttgaaaa tcaataggat tatttaaaac aacggcatct ttttgtcccc
     4741 aacacaaata attggcgcct ttggaattgg aattaaaata agcaattgac tgattgttca
     4801 tacccaacga aggtaagcaa ataatcagat tattttattg tccgttggga ttaattccct
     4861 tttctttcat ttgtttcatc agtgctttga gttgctctcc ttccaccaat cttccaaaat
     4921 gataatacct tctttggcct tctttaccat cttctttatt gtagccgatg aaccaaccgt
     4981 gcatttttcc atccaaataa tgctccacat acattttctg accactcaat tcataaccaa
     5041 cccattcacc tgtgcgcttg tggttcttgt attgccctgt taatttaagt gttcgtgtag
     5101 gtttggtggg ataatattct gcaaacttcc cttctggttt tccgtccacg tagttgtatt
     5161 ctgaaattac tgtttcttga agatcttctg gatcggaagg aaggtaatac atttttttca
     5221 ttccgttgag cacatcgttt ttataggttt ctgtcatagt gatgcgtcca ccctcgttga
     5281 aactggtcca cacactatct tttttcatgt tgcgatagat gccgtgcgtc ataagttttc
     5341 cattttcata ataaaaaaag gcttcagaac gaggagttgt ttcactgtgt ttgattactg
     5401 ctttgacttt attgctggga tagcggtagg taaatgtacc aattggttta tcgtttttaa
     5461 atacaccttg atacatagca actttgctat tgggatataa ttttacccat tgcccttgtt
     5521 tttttccttt ggcatctgtt tggttgattt gcgcaaacgt aaaacaaaca acaaaaacac
     5581 ttaaaacaac actaatgatt ttcattttcc actaatttta aaaattcaaa caagctgtca
     5641 acagtaacat ctgcttgtat tttgggtaaa tcgttcccta caattcgcac ggttttcatt
     5701 ccaagattca taccaaattg aatgtctgaa tcgctatctc ctaccattat tgctttagaa
     5761 aaatccactt ctggaaaatc tttttttgcc aacaatccca tgccaatatt gggttttcgc
     5821 aagttgctat tttccttagc caatgcaggt gcgtgatagc agttagaaat ttttccgccc
     5881 gcttgttcca cttcattgac catataacga tgaatgactt gaaggttact ttctgtcatt
     5941 attcctttac caattccttg ttgattggta accacaaaaa tatgttggaa atgctgtgaa
     6001 agtccagcaa ttgcttcttt tacgttgggt aaaaattgaa attcttcgat ggtttcaatg
     6061 tatccaccca tttttcgatg gttgataacg ccatctctgt ccagaaacaa ggtccaagaa
     6121 gcatcaatgt tccaatttac catgcttaca attctttttc tatcaagtag gtattgcgag
     6181 tagatgaatt tctaccaatt aactcggcga tgaatccagc taagaaaaac tgaacaccaa
     6241 taatcatggc cactagtgca atataaaact cggttctatc ggcaatttta atggcgtgtg
     6301 tgttgaaaaa caatttatca acggccaaat aaaaagaaaa gccaaatccg atg
//
LOCUS       DAOQVQ010000091         6334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13562_length_6334_cov_3.769072, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000091 DAOQVQ010000000
VERSION     DAOQVQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13562_length_6334_cov_3.769072"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PKN22_04175"
     CDS             complement(<1..925)
                     /locus_tag="PKN22_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41932.1"
                     /translation="MKLNKNNMKSSITLAMIGLSMTVAFGQTNENLIPNGSFESVAKD
                     PKKLGSIESATGWYSPTGAKADLFLPSKKTPDIGSPQNIYGKEDAKDGSNYAGFVAFS
                     HNNKMPRSYVSNKLTVPLKKGEKYCVQFYVAMAEASMYASNQIGGLFSKKAYGTEAKE
                     SIIEKTHILNENNKIFNAPYGWDQVCGVYTAEGGEKFFTIGNFTNNEDTKNSRNKKDA
                     KSKIAPIPAAYYYLDNISVTLMDEDNPCECAGAVDPTAAFSKTVYQKALNIAADLPLD
                     KKVEALSVYFGFGKTDITAAGEEALKMLVKLL"
     gene            complement(1099..1740)
                     /locus_tag="PKN22_04180"
     CDS             complement(1099..1740)
                     /locus_tag="PKN22_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS41933.1"
                     /translation="MKKLKPEVLNALKVINLETLNPRSQFIYNSLKSGKNLFIHTFNF
                     NEESTALMVAIFNKVNQEYEGSPRAIYVTDSSQNAIHCFEDYKKVCRKLDITVDLAHD
                     KGNMIQQRNDIFDGTEIIVGNPKRIYDLYIKNGINTALLDLFIVDNLAECLKDNKQAE
                     LKRIIDSLEKKTQVVLLSNSLSNKVQNFVDNLEIQYTFLDVEETFEDEAMNND"
     gene            1902..2549
                     /locus_tag="PKN22_04185"
     CDS             1902..2549
                     /locus_tag="PKN22_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS41934.1"
                     /translation="MTPQERKKGKKEDVRKLILDAAKKLFIEQGYEATSMRNIATEIN
                     YSPTTIYLYYKDKGDIIYALHQEGFTILKMLFMPLATVEAPFERLKALGRTYLNFAKE
                     YPEYYEVMFMMKEPMDHLDKTANEELWEGGKEMFGFIISTIVECQEKGFFSKDNPHYI
                     ALQAWAMVHGLCSLYLTTRIHKIGMENEAALSADALLDNAYKSFVSFIESKKMNV"
     gene            2546..3898
                     /locus_tag="PKN22_04190"
     CDS             2546..3898
                     /locus_tag="PKN22_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNS41935.1"
                     /translation="MKNRIMLVFFALGFWLTSFGQNTSQLDQYIEQGLKSNLVVQQRS
                     LSLENALNALKQARGLFLPTFDFQAQYLSAEGGRYTELPLGDFLNPIFNTVNGLMGAP
                     LLPEVKNERINFLPNNYYDARFRLSVPILNTDIIHNNSISKKQLAIKENELEIYEREL
                     VKEIKVAYYHYLAAVKAQQVYHNSLTIAEESKRVNEKLVQAGNGLPAYILRSESEIEQ
                     IKSQLKTVELQANSLQRYFNALLNREENAEIIVENSESIIPFVDKAELTAQNREELNS
                     LQLAVALRQDLVKMKKHAFVPTLGGFLDLGSQAEDFKFNDKSMYYMVGLQLNIPIFQG
                     NRNNLKIKEAKNELEIAKLQEASVAQQLEVAVNNAYNEVLTEKSKYESSLKQLEMAET
                     YHRLIEKGYAAGTNTYLESIDARSQLSSAKLATNINYYQFLAALARLERETASYPLTK
                     "
     gene            3912..4952
                     /locus_tag="PKN22_04195"
     CDS             3912..4952
                     /locus_tag="PKN22_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS41936.1"
                     /translation="MKNIQFFAVIVFLTVLTSCKNKEEQATFNLNEKVLVSIAPIQKE
                     EGNMVVEASGLFTTDDETLLSFKNGGLIERIYVKEGDFVRKGQVLATLNQTEINAKAA
                     QLKAALDKAERDYNRAQKLYKDSVATLEQFQNAQTQLEVMQQDWNTMAYNLQHSQIKA
                     KSDGYVLLKLANEGQMVGPGMPVFQMNGAGEGEWVVKVGVSDFQWASINEGDTAIIET
                     DAYANKINAKVIQKSEGINPNSGTFTITVQPNSMKGLKLASGMFAKVKIFCNATKSWF
                     VPYEALLDGNNGKGYVFVTNDKKTAKRVAVQLASLQSESVMIKSGLENYKYLIVSGSP
                     YLKDGSLIEIVY"
     gene            4976..>6334
                     /locus_tag="PKN22_04200"
     CDS             4976..>6334
                     /locus_tag="PKN22_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS41937.1"
                     /translation="MNLIKAPIKNYQLTLILVLMIVIVGVSTLLNMPRAEDPDMEAPY
                     FPVVVVYPGASPKDMEQLVAKPLEARFNSLDKIKHIKTKIDNGLVFMVVEYEYSANYN
                     AKYQEVVRELGAAQQQDLPEGIYRAEVIEIDPTNVNIIQGALISENASQAQMKKLSDE
                     LKGELEKVPSLKNVTIHGISEPLIRVDLNEGRLAQLKIPASQIMDAIGAEAMNIPGGS
                     VHAGQKSFSVKAGGGYTSIEQIENTVVSSYQGRNITLKEVAKVYSSFSPETYITRLNG
                     FKCLFVTAAQKKGMNISNTQDEYQKVVEAFKEKLPENVDLVLNFEQADNVKSRLSGLG
                     KDFLIAIGLVLITLLPLGTRASIVVMIAIPLSLATGIVILNAFGFSLNQLSIVGFVVA
                     LGLVVDDSIVVVENIERWLREGNSPFDAAVKGTKQIALAVVGCTATLAVAFMPLMFLP
                     EKA"
BASE COUNT         2036 a         1016 c         1279 g         2003 t
ORIGIN      
        1 ttaacaattt caccaacatt ttcaacgctt cttcgccagc tgctgtgatg tctgtttttc
       61 caaatccaaa ataaacgctt agtgcttcta cttttttatc caacggcaaa tctgcagcaa
      121 tgttcaacgc tttttggtaa actgtttttg agaaagcagc tgttgggtca actgctccag
      181 cacattcgca tggattgtct tcgtccatca acgtaactga aatattatcc aaataataat
      241 aagctgctgg aataggtgct atttttgatt tagcatcttt tttatttctt gagttttttg
      301 tgtcttcatt attggtgaag ttaccgatag taaagaattt ttcgccacct tcagctgtgt
      361 aaacaccaca cacttgatcc catccataag gtgcattgaa tattttgttg ttttcgttta
      421 agatgtgtgt tttttcaatg atactttctt tagcttctgt tccataagct tttttggaga
      481 ataaacctcc aatttggtta cttgcataca ttgatgcttc tgccatggca acgtaaaatt
      541 gcacgcagta tttttcaccc ttttttaatg gaactgttaa cttgttagac acataagaac
      601 ggggcatttt gttattgtgc gagaaagcaa caaatccagc atagttagaa ccgtctttgg
      661 catcttcttt accataaatg ttttgcggtg aaccaatgtc tggtgttttt ttacttggta
      721 aaaataaatc ggctttagca cctgtaggtg aataccaacc tgttgcactt tcaattgaac
      781 ctaatttttt ggggtctttt gcaaccgatt caaaactgcc gtttggaatt aaattttcat
      841 tggtttgccc aaaagcaacg gtcattgaaa gaccaatcat tgctaatgtg atagaacttt
      901 tcatattgtt tttgttgagt ttcataattt ttaagtttta aacgcttggt tttaatactt
      961 cattcatcaa atatacaatt agtctttaat ttaacagtgc caacgttgta ttttttgtga
     1021 attttgtaat aaaatgccaa tatttcacga aaaacagttt accaattggt atgccaaact
     1081 attttttata cgttagaatt aatcgttgtt cattgcctcg tcttcaaaag tttcttctac
     1141 atcaaggaag gtgtattgaa tttctaaatt gtctacaaaa ttttgtactt tattggataa
     1201 gctgttggat agtaaaacta cctgcgtttt tttctccaaa ctgtctatga ttctttttaa
     1261 ttcggcttgc ttgttgtctt ttaaacattc ggctaaattg tcaacaataa acaaatctaa
     1321 taatgcagtg ttgatgccgt ttttaatgta taaatcgtaa atgcgcttcg gatttccaac
     1381 tataatttct gttccgtcaa aaatatcatt gcgctgttga atcatgttgc ctttgtcgtg
     1441 cgctaaatca acggtaatgt ccagttttct gcaaactttt ttataatctt caaaacagtg
     1501 aatagcattt tgagagctgt cagtaacata aattgctctt ggagaacctt cgtattcttg
     1561 gtttactttg ttaaaaatag caaccatgag agcagttgac tcttcattaa aattaaacgt
     1621 atgaataaaa agattttttc ctgattttaa agaattgtaa ataaattgag atcttggatt
     1681 cagtgtttct aagttgatca cttttaatgc atttagaacc tctggtttta actttttcaa
     1741 aacgttgtat tttttggcga atttacaaaa tattcaatag aactttggat ttataacttt
     1801 aaatttcggt gttattacat gtaagttatt taaaacaagt tgtttatgat ttttattaaa
     1861 tatttactta acagtgttaa tattttgatt aaatttgtag catgacacca caagagcgaa
     1921 aaaaaggaaa aaaagaagat gtacgcaaat tgattctgga tgcagcaaaa aaactgttta
     1981 tagaacaagg gtatgaagca acatctatgc gcaatattgc aacggagatt aattatagtc
     2041 caacaaccat ttacttgtat tacaaagata aaggtgatat catttatgct ttgcaccaag
     2101 agggatttac cattttaaaa atgttgttta tgcccttggc aaccgttgaa gcgccttttg
     2161 aacgtttgaa agctttgggc agaacctatt tgaactttgc aaaagaatat ccggagtatt
     2221 acgaagtgat gttcatgatg aaagaaccga tggatcattt ggataaaacg gccaatgaag
     2281 agctgtggga aggaggaaaa gaaatgtttg gatttattat ttcaaccatt gttgaatgcc
     2341 aagaaaaagg ttttttctca aaagacaatc cgcattacat tgccttgcaa gcgtgggcaa
     2401 tggtgcatgg attatgttcg ttgtatttaa caacacgaat tcacaaaatt ggaatggaaa
     2461 acgaggcagc attatcggcc gatgcattgt tggataatgc ctataaatcg tttgtatctt
     2521 ttatagaatc taaaaaaatg aatgtatgaa aaatagaata atgcttgtat tttttgcact
     2581 tggcttttgg ttaacatcgt ttggtcaaaa caccagtcaa ttggatcaat acatagagca
     2641 aggattgaaa agcaatttgg tggttcagca acgttcgtta tcgctcgaaa atgcactcaa
     2701 tgcgttgaag caagcacgtg gattgttttt gccaacgttt gattttcagg cgcaatacct
     2761 ttcggcagaa ggtggacgtt atacagaatt gcccttgggt gattttttaa atcccatttt
     2821 taatactgta aatggtttga tgggagcgcc actattgccc gaggttaaaa atgaacgcat
     2881 caatttcttg cctaataatt attacgatgc gcgttttcgt ttgtcagtgc ctattttgaa
     2941 tacggatatc attcacaaca attcaattag caaaaaacaa ttggccataa aagaaaacga
     3001 gttggaaatt tatgagcgtg aattggtcaa ggaaattaaa gtggcttatt atcactattt
     3061 agctgctgta aaagcgcaac aggtttacca caattcgttg acaattgcag aagaaagcaa
     3121 gcgagtgaat gaaaaattag tgcaagctgg aaatggtttg cctgcctata ttttgcgttc
     3181 tgaaagcgaa attgaacaaa taaaatcgca actaaaaacg gtggaattgc aagctaattc
     3241 gcttcaacgt tacttcaatg cgttgctcaa tcgcgaagaa aatgctgaaa ttattgttga
     3301 aaattcagaa tccattattc cttttgtgga taaagcagaa ttgacagctc aaaatagaga
     3361 agaactcaat tcgttgcaat tggctgtggc tttgcgtcaa gatcttgtaa aaatgaaaaa
     3421 acatgctttt gttccaacct tgggtgggtt tttagactta ggttcacaag ccgaggattt
     3481 taaatttaac gataaatcaa tgtattacat ggttggtttg caacttaata ttccaatttt
     3541 tcagggcaat agaaataatt tgaaaatcaa agaagctaaa aacgaattgg aaattgcaaa
     3601 attgcaagaa gcatcggtgg cgcaacaatt agaagtggct gtaaacaatg catacaacga
     3661 agtgttgaca gaaaaatcaa aatacgaatc gtcgctcaag caattggaaa tggcagaaac
     3721 ctatcatcgt ttgattgaaa aagggtatgc agcaggcacc aatacgtatt tggaaagcat
     3781 tgacgcacgc tcgcaattgt cgtcggcaaa attagcaacc aatattaatt attaccaatt
     3841 tcttgcagca ttggcaaggt tggaaagaga aacagcatcg tatccattaa caaaatagaa
     3901 aattagcaat tatgaaaaat atacaattct ttgcagttat tgtgttctta acagtgttaa
     3961 caagttgtaa gaacaaggaa gaacaagcaa cgtttaattt aaacgaaaaa gtgctggtat
     4021 ccattgcgcc cattcaaaaa gaagaaggta atatggtggt ggaagcatca ggtttattta
     4081 caaccgatga tgaaaccttg ctttcgttta aaaatggagg tttaattgaa cgaatttatg
     4141 taaaagaagg tgattttgtt cgcaaagggc aagtattggc aacgttgaat cagaccgaaa
     4201 ttaatgcaaa ggcggcgcaa ctaaaagcgg ctttagacaa ggcagaacgc gattacaacc
     4261 gcgcacaaaa gttatacaaa gacagtgttg caaccttgga acaatttcaa aatgcacaaa
     4321 cacaattaga ggtgatgcaa caagattgga atacgatggc atacaacctt caacattcgc
     4381 aaattaaagc aaaatcggat ggatatgtgt tgttgaaatt ggccaatgaa gggcaaatgg
     4441 tgggacctgg aatgcctgtt tttcaaatga atggagctgg agaaggtgaa tgggttgtaa
     4501 aagtaggagt ttctgatttt caatgggctt ccatcaacga gggagatacg gctatcattg
     4561 aaacggatgc ttatgccaat aaaataaatg caaaagttat tcaaaaatcg gagggaatta
     4621 acccaaattc tggtaccttt acaataactg ttcaacccaa ttcaatgaaa ggtttaaaat
     4681 tggcatcggg gatgtttgca aaagtgaaga tattctgcaa tgcaaccaaa tcgtggtttg
     4741 tgccttatga agcattgttg gacggcaata atggaaaagg ttatgtattt gtaaccaacg
     4801 ataagaaaac agcaaaaaga gtggcggtgc agttggcttc gttgcaatcc gaaagcgtga
     4861 tgataaaatc ggggttggaa aactacaaat acttaattgt ttctggaagt ccgtatttaa
     4921 aagacggctc acttattgaa attgtttact aacttgtgac tattattatt gttgtatgaa
     4981 tctaattaaa gcaccgataa aaaattacca gttgacactc attttagtgt taatgattgt
     5041 tatcgttggg gtttcaacct tgctaaacat gccaagagcc gaggatcctg acatggaagc
     5101 cccttatttt cctgttgtgg tggtgtatcc tggagccagt ccgaaggata tggagcaatt
     5161 ggtggcaaaa ccattggaag cacgttttaa cagtttggat aaaataaaac acattaaaac
     5221 caaaattgac aacggtttgg tgttcatggt ggttgaatac gaatattcgg caaattacaa
     5281 cgcaaaatac caagaagtag tgcgcgaatt aggggctgca caacaacaag atttgcccga
     5341 aggaatttat cgtgcagaag ttattgaaat tgatccaacc aatgtaaata ttatccaagg
     5401 tgcattgatt tctgaaaatg cttcgcaagc tcaaatgaaa aaattgtcgg atgagttaaa
     5461 aggagagttg gaaaaagtgc catcgttgaa aaacgtcacc atacatggta tttcagaacc
     5521 attgattcgt gttgacttaa acgaaggtcg cttggcacaa ttaaaaattc ctgcttcgca
     5581 aatcatggat gcaattggag ccgaggcaat gaacattccg ggcggtagtg tgcatgcggg
     5641 acaaaaatcc tttagtgtaa aagctggtgg aggatacact agcattgagc aaattgaaaa
     5701 taccgttgtt tctagctatc aaggacgaaa cattactctc aaagaagtgg caaaagttta
     5761 ttcaagtttt tcaccagaaa cctatatcac ccgtttaaat ggctttaaat gtttgtttgt
     5821 tactgccgcg cagaaaaaag gaatgaacat cagcaacacg caagatgaat accaaaaagt
     5881 tgtggaggct tttaaggaaa aattgcccga aaacgtggat ttggtactca attttgaaca
     5941 agccgacaat gtgaaaagtc gcttgagtgg tttgggtaaa gactttttga tagctatagg
     6001 tttggttttg attactttat tgccattggg tacacgtgca tccattgtgg tgatgattgc
     6061 tattccgcta tcgttggcaa cgggaattgt gattttaaat gcctttgggt tttcgctcaa
     6121 ccaattgagt attgttggat ttgtggttgc acttggtttg gtggttgacg atagtattgt
     6181 tgtggttgaa aacattgaac gttggttgcg cgaaggtaat tcaccctttg atgcagctgt
     6241 gaaaggaacc aagcaaattg cccttgcagt agttggttgt acagctactt tggcagttgc
     6301 gtttatgcct ttaatgttct tgcccgaaaa agcg
//
LOCUS       DAOQVQ010000092         6325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13584_length_6325_cov_3.436204, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000092 DAOQVQ010000000
VERSION     DAOQVQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13584_length_6325_cov_3.436204"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3092
                     /locus_tag="PKN22_04205"
     CDS             <1..3092
                     /locus_tag="PKN22_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS41938.1"
                     /translation="SSQNPTIPNATTAASGTYTVVVTGANGCSANATVSITVNPLPNV
                     SSNVNSPVCVGSAINLTSTAGGSSYSWTGPNGFTSSVQNPTISSATTAMSGLYTVTMT
                     NASGCSNSSSISVTVNDVPTVTVSPNTSVCEGNAINLTSNGGSSYSWTGPNGFTSTLQ
                     NPTILNAGVSNTGTYVVTVTNGSGCSAQGSTTITVNPRPAITVSASPTTICSGNSSTL
                     LVTGPAGTTYNWDNGHVGSSQSVSPTITTIYTVTATASNSCQTIDQITINVNPSPTYS
                     ASLTNPTCGNLNGQIVLTANNGTPLYSYSINNGATSQASGTFSGLGAGTYNILITDAS
                     GCTVTGTENLSNLGAPTINNATPTNPSCNGACDGSISIVASGGTGTLTYQWYDQANNP
                     IGFNSTSISSLCAGSYSVTVTDANNCVASTNISITNPPLVDASFTLTDYCEGSSNNAT
                     NIATPGGTFAFNPNPMDGSSINGSTGEITNGIGGTTYSITYTTPGACPNTSTQTVTVT
                     SPVDPGVISGPTLICPTGTSTYTSNGDAGGIWATSNPSIATIDGSGNLTAVSTGTFDV
                     TYTLFGTGGCPNDNATLSVTIDDNTSPVFNACVNDTTMSSAIVGSCDIIVPDFTSSNQ
                     IDVTDNCSDLASGTIVITQNPTAGTTLGAGTHTIVLTATDAYGNNSTCSFNLTVTDTI
                     APVFNGGCLNDTTIYSSNNACDIVVPYFTNNSQLNVTDNCSDVASGTLVVTQSPTAGS
                     TLGVGSHTITLTATDNSGNTSTCTFNLSVQDTISPSVIINVASPIVCENDTVSWTETI
                     SDNCVVQTVVSSHNSNTVFPVGTTTVTYTVTDVNGNVTQTTFDITVIENPIITLNGND
                     TVTVCSGLNTTLSVANPDNNTTYTWYFGNSQLGTGSSYTVESVLPADGGVYTVIATNA
                     NGCTSSHEILLIVEACSIVIPEVFSPNGDGQNDKFYIEGLDGYPNSKVWIYNRWGSEV
                     YYHENYQNDWDGKSISKLNVAGDDLPEGTYYYILQLGGNEGQPNAGEMHKGYVYLKR"
     gene            3143..4072
                     /locus_tag="PKN22_04210"
     CDS             3143..4072
                     /locus_tag="PKN22_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNS41939.1"
                     /translation="MKKIKILFAIALMIGSANQLQAQQEAQFTQYMDNMLYYNPAYAG
                     SRDMMNISVLHRQQWAGFKGAPMSTTFSLHTPLRYDNIGLGFSLLNDKLGPTNSTWFN
                     ADFSYSFKFKKNNSRLSFGVKGGINLLNGDLLNLVKHDQNDALVNVRFKNEVQPNVGV
                     GIYYQSTQWFAGVAIPRIIDNLKKAGDLSSIEYIAQRHYYLTLGGYIKANRMLKIRPS
                     TMLKITEQAPLALDVSLAFIMYDKVWIGANYRLLESAGIMFQYQFSNQFKIGYSFDYS
                     TTKLRSYNYGTHEILLSYDLLFKSKSLTSPRYF"
     gene            4112..>6325
                     /locus_tag="PKN22_04215"
     CDS             4112..>6325
                     /locus_tag="PKN22_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS41940.1"
                     /translation="MKYLILLFTMISTTFLGLSQNARLKYADRMYEQKAYYYASEAYE
                     DVIARKVDSATVAVKIADCYDKIGNRKKAAVWYNFLNRQNLLTKEQHYRLVLIEHGLE
                     EYNKSSELLASFESKYGANESTKALNKSLNELKKDNDQFEIKTLKVNTTASDMSASFY
                     NDKNILLASSKRRSKAVMSLHSWTGNYFYDLYSAPINENGEIGKMKLLKAGGKTKYHD
                     GPAVYNAKNGYVYFTRNNFLNGKKGYDDQKSVRLKIYKAKYDGKKLVDVTELSINDNA
                     FSSAHPAISEDGKKLFFASDRPGGFGGMDIYSVGLDNDGNTVGSPINLGQRVNTNQHE
                     LFPSFNSTENLLFFSSEGHFGLGGLDVFMAKMTKTGDVTSIENLGSPINSTFDDFSFT
                     ANPQQTKGFFTSNRNGGKGDDDIYGFNQKFPFRNSAVLKGNAINLLTKEKVENATVYI
                     ADKNGVIVDSTTSKDDGSFEFSLATINDDFSIVGQKENFVEGKQSVKYDATKMEYQQD
                     VELMPLIDYYFTGVVKDKATKELLSGVQTSISDNLKNELFESVKTNDNGAFKSTILPY
                     KYQDKISYEFKLEKDGYITKTVALADILALQQEINVNGVLSLELTKIEVGKTDLNDVI
                     AINPIYFDLNKSDIRPDAAVELNKIVEVMKQNPGMVIELGSHTDSRGSDASNLSLSDR
                     RAKSSAKYIISQGIAKDRIYGKGYGESKLKVSDAEIAKAKTEEEKETLHQQNRRTE"
BASE COUNT         2214 a         1036 c         1145 g         1930 t
ORIGIN      
        1 gttcttctca aaatccaaca attccaaatg ctacaactgc agcaagcggt acatacaccg
       61 tagttgttac aggtgctaat ggctgttctg caaatgcaac ggtttctatt acagtaaatc
      121 cgttaccaaa tgtttcaagc aacgttaata gccctgtttg cgttggttct gcaattaatt
      181 taacatcaac tgctggtggt tcaagctata gctggactgg tccaaacggt tttacttcaa
      241 gtgttcaaaa cccaacaatt agtagtgcta ctacagcaat gtctggttta tacactgtaa
      301 caatgactaa tgctagtgga tgttcaaatt cttcatcaat ttctgttaca gtaaatgatg
      361 taccaacagt tacagtatct ccaaacactt ctgtttgtga aggaaatgca ataaatttaa
      421 cgtcaaatgg cggaagctca tatagctgga ctggaccaaa tggatttaca tccacattac
      481 aaaatcctac aattttaaat gcaggagttt caaatacagg aacttatgta gtaacagtta
      541 caaatggttc aggatgtagt gctcaaggtt caaccacaat tactgttaat ccacgacctg
      601 caattactgt tagtgcaagc ccaacgacaa tatgctctgg taattcaagt acacttcttg
      661 tgacaggacc tgcaggtaca acatacaatt gggacaatgg acatgttgga agttcacaat
      721 ctgttagtcc tacaataaca acaatctata cagttactgc aactgcatca aatagttgtc
      781 aaactataga tcaaattacc ataaatgtta acccttctcc aacatactcg gcatcattaa
      841 ctaaccctac ttgtggtaat ttgaatggtc aaattgtgtt aactgcaaac aatggaactc
      901 ctttatacag ttattctatt aataatgggg ctactagtca agcaagtgga acgttttcag
      961 gattgggagc aggcacatat aacatcttaa ttacagatgc ttctggatgt actgttacag
     1021 gtactgagaa tttaagcaat ttaggcgctc ctacaatcaa caatgctacg ccaacaaacc
     1081 cttcttgtaa tggtgcttgt gatggttcaa tatctattgt agcaagtggt ggaactggaa
     1141 cgcttacata tcaatggtat gatcaagcaa ataacccgat tggttttaac tcaacatcaa
     1201 tttcaagttt atgtgctggt tcttatagtg taactgttac tgatgctaat aactgtgtag
     1261 cttcaacaaa tatttctata accaaccctc ctttagttga tgcttctttc actttaacag
     1321 attattgtga aggatcttca aacaatgcaa caaacattgc aactcctgga ggaacatttg
     1381 catttaaccc aaatccaatg gatggatcaa gtattaatgg ctcaactggt gaaataacaa
     1441 atggaattgg aggcacaacc tattcaataa catacactac tcctggagct tgtccaaata
     1501 catcaactca aacggttaca gttacaagtc ctgttgaccc aggtgtaatt agtggcccaa
     1561 cattgatttg tccaactgga acatctacct acacatcaaa cggtgatgct ggtggaatat
     1621 gggcaacatc aaatccaagt attgctacaa ttgatggaag tggtaattta acagctgttt
     1681 ctaccggaac atttgatgta acatacacat tatttggaac aggtggttgt cctaatgaca
     1741 atgcaacgct ttctgttacg atagacgaca atacctcacc agtatttaat gcttgtgtta
     1801 acgatacaac aatgtcgtct gcaatagttg gtagttgcga cattattgtg ccagacttta
     1861 catcgagcaa ccaaattgat gtaacagata actgtagtga tttagcaagt ggaacaatag
     1921 taattacaca aaatccaact gctggaacaa cattaggagc tggaacacac acaattgttt
     1981 taactgcaac agacgcatac ggaaacaaca gtacttgttc gttcaactta acagtaaccg
     2041 atacaattgc acctgtattt aacggtggtt gtttaaacga tacaactatt tattctagta
     2101 ataatgcatg tgatattgtt gttccttatt ttacaaataa ctcacagtta aatgttactg
     2161 ataattgtag tgatgtggca agtggaacgt tagttgtaac tcaaagtcca actgctggtt
     2221 caacattagg tgttggttcg catacaataa cattaacagc aactgataat agtggaaaca
     2281 cttctacctg tacatttaat ttaagcgtac aagataccat ttcaccatcc gttattatta
     2341 atgttgcatc tccaatagtt tgtgaaaacg atacagtatc ttggactgaa actatttctg
     2401 ataattgtgt tgttcaaact gttgtaagta gccataacag caatacagta ttcccagttg
     2461 gaacaacaac tgttacttat accgttacag acgttaatgg caatgtaact caaacaactt
     2521 ttgatattac tgtaattgag aaccctataa tcacattaaa tggaaatgat actgttacag
     2581 tttgttcagg attaaacaca actttaagcg tagctaatcc agacaataat acaacttata
     2641 catggtattt tgggaactca caattaggaa ctggaagttc ttacaccgtt gagtcagtat
     2701 tacctgcaga cggtggtgta tatactgtaa ttgcaacaaa tgcaaatgga tgtactagtt
     2761 ctcatgaaat cttacttatt gttgaagctt gttcaattgt tatcccagag gtgttctcac
     2821 caaacggcga tggtcaaaac gacaaattct atattgaagg attggacggt tatcctaact
     2881 ccaaagtttg gatatacaac cgttggggaa gcgaggtgta ttatcacgaa aattaccaaa
     2941 atgactggga tggtaagtct attagcaaac tcaatgttgc aggagatgat ttaccagaag
     3001 gtacatatta ctacatactt caacttggtg gtaatgaagg acaaccaaat gcaggtgaaa
     3061 tgcacaaggg ttatgtttac ttaaaaagat aagccttagt taacgttgtt ttatttacta
     3121 tctaaattca atatcaagag atatgaaaaa aattaaaata ttattcgcta ttgctttaat
     3181 gattggaagc gctaaccaat tacaagcaca acaagaagca cagttcacgc aatacatgga
     3241 caatatgctt tattacaatc ctgcttatgc aggtagtcgt gatatgatga acatatccgt
     3301 tttacatcgc caacaatggg caggttttaa aggtgctcca atgagtacaa cttttagctt
     3361 gcatacgcca ttaagatatg acaatattgg cttaggtttt agtttattaa atgataaatt
     3421 aggacctaca aactctacat ggtttaatgc agatttttcg tattcattta agtttaagaa
     3481 aaataactca cgtttatctt ttggtgtaaa gggtggaatc aacttgttaa acggtgattt
     3541 attaaacttg gtaaaacatg accaaaatga tgcattggtt aatgttcgct ttaaaaatga
     3601 agttcagcca aatgtggggg ttggtattta ttatcaatca acacaatggt ttgcaggagt
     3661 tgctatacca agaattattg ataatttaaa aaaggcaggg gatttatcat ctattgaata
     3721 cattgctcaa cgtcactatt acttaacatt gggtggttac attaaagcca atagaatgtt
     3781 aaaaataaga ccgagcacga tgctaaagat aacagaacaa gctccgcttg cattagatgt
     3841 tagtttagct tttataatgt atgataaagt ttggattggt gctaattata gattattgga
     3901 aagtgctgga attatgttcc aataccaatt ttccaatcag tttaaaattg gatactcatt
     3961 tgattattca acaactaaat tgcgttcata caattatgga acacatgaga tattgttgag
     4021 ttatgactta ttgttcaaga gtaaatcatt gacatcacca cgttattttt aatagaattt
     4081 gcactaaata cacaacgaaa acattaaaga tatgaagtat ttaatattat tattcacgat
     4141 gattagtaca acctttttag gattgtcgca aaatgctcgt ttgaaatatg ccgatagaat
     4201 gtatgaacaa aaagcatatt attatgccag tgaagcgtat gaagacgtaa ttgcaagaaa
     4261 agtagattct gctacagttg cagttaaaat agctgactgt tatgataaaa ttggcaatag
     4321 aaaaaaagct gcggtatggt ataatttcct taatcgtcaa aacttgttga caaaagagca
     4381 acattaccgt ttagttttga ttgaacatgg tttagaggaa tacaataaat caagcgagtt
     4441 attagcaagt tttgaaagca aatatggagc taatgaatca acaaaagcgc tcaataaatc
     4501 gctaaatgaa ctcaaaaaag ataatgacca atttgaaata aaaactctaa aggttaacac
     4561 aacagcttct gacatgagtg ctagctttta taatgacaaa aacatcttgt tagccagttc
     4621 aaaaagacgc tctaaagcag ttatgagctt gcattcatgg acaggaaatt atttctatga
     4681 tttatacagt gcgccaatta atgaaaatgg agaaattggt aaaatgaagc tattaaaagc
     4741 tggtggtaaa acaaaatacc acgatggtcc tgctgtttac aatgcaaaaa acggatatgt
     4801 ttattttaca agaaataatt ttttaaatgg gaaaaaaggg tatgatgatc aaaaatcagt
     4861 gcgcctaaaa atttataaag ctaaatatga tgggaaaaaa ctggttgacg tgactgaatt
     4921 atctattaac gataatgcct tttcatcagc acatcctgct atatcagaag atggtaaaaa
     4981 attgtttttt gcttcggata gaccaggagg atttggcggt atggatattt attctgttgg
     5041 tttagataat gatggaaaca ctgttggttc accaattaac ttaggacaaa gagtaaatac
     5101 aaaccaacac gaattatttc cttctttcaa ttctacagaa aacttgttgt ttttctcatc
     5161 tgaagggcat ttcggattag gtggattaga cgtattcatg gctaagatga ccaaaactgg
     5221 tgatgttaca agtattgaaa acttaggatc accaatcaat agtacttttg atgatttttc
     5281 atttacagct aatcctcaac aaactaaagg atttttcact tccaatagaa atggtggaaa
     5341 gggtgacgat gatatttacg gatttaatca aaaatttcct tttagaaata gtgctgtgtt
     5401 aaaagggaac gctatcaact tgcttacaaa ggaaaaagtt gaaaatgcca ctgtttatat
     5461 tgctgataaa aatggagtaa tagtagatag cacaacttca aaagatgatg gaagttttga
     5521 attttcattg gcaactatta atgatgattt tagtattgtt ggtcaaaaag aaaattttgt
     5581 tgaaggaaaa caatctgtga aatatgacgc aactaaaatg gaataccaac aagatgtgga
     5641 attaatgcca ttaattgatt attacttcac tggtgttgta aaagataaag ctacgaaaga
     5701 acttttgagt ggtgttcaaa catcaatttc tgacaactta aaaaatgaat tgtttgaaag
     5761 cgtaaaaacg aatgacaatg gagcttttaa atcaactatt ttgccttata agtaccaaga
     5821 caaaatttct tatgagttca aattagaaaa agatggttac ataactaaaa ctgttgcgct
     5881 tgccgatata ttagctttac aacaagaaat aaatgttaat ggagttttat cacttgaatt
     5941 aacaaaaatt gaagttggaa aaacagactt aaacgatgtt attgctatta accctattta
     6001 ttttgactta aataaatcgg atattcgtcc agatgcagct gttgagttaa acaaaattgt
     6061 tgaagtaatg aagcaaaacc ctggaatggt aattgagctc ggctcacaca ctgactcacg
     6121 tggttctgat gcatcgaatt taagtttgtc tgaccgaaga gctaaatcat ctgcaaaata
     6181 catcatttca caaggaattg caaaagatcg tatttatggt aaaggttacg gtgaatcaaa
     6241 actaaaggtg agcgatgcag aaattgctaa agctaaaaca gaggaagaaa aagaaactct
     6301 tcaccaacaa aatagaagaa ccgag
//
LOCUS       DAOQVQ010000093         6320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13602_length_6320_cov_3.687310, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000093 DAOQVQ010000000
VERSION     DAOQVQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13602_length_6320_cov_3.687310"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..593)
                     /gene="rpiB"
                     /locus_tag="PKN22_04220"
     CDS             complement(162..593)
                     /gene="rpiB"
                     /locus_tag="PKN22_04220"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNS41941.1"
                     /translation="MIIPIGCDHAGFQLKQEIIKHLQSRGYEVKDFGCYSEESIDYPD
                     YGHPVAEMVEQNKGMLGIVICGSGNGINMTVNKHQGIRSALCWTPEISKLAREHNDAN
                     IIAMPARFVSTETAIEMVNNFLDTPFEGGRHQKRIDKIPFC"
     gene            752..2452
                     /gene="lysS"
                     /locus_tag="PKN22_04225"
     CDS             752..2452
                     /gene="lysS"
                     /locus_tag="PKN22_04225"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNS41942.1"
                     /translation="MSFIELSEQELQRRETLNQIRQMGIDPYPAALYPVDDYAKNIKE
                     NFEDKKQVCIAGRMLSQRIMGKASFVELQDSTNRIQVYFNRDEICPGDDKSLYNDLFK
                     RFLDLGDFVGVKGYVFKTQVGEISVHVQEFTLLSKSLKPLPLPKIDAEGKVYDALTDP
                     ETRYRQRYADLVVNPHVKEVFVKRTKLFNAMRQFFNDAGYMEVETPILQSIPGGAAAR
                     PFITHHNALDIPLYMRIANELYLKRLIVGGFDGVYEFSKNFRNEGMDRTHNPEFTAME
                     IYVAYKDYNWMMDFTERLLEHCAMAVNGTTKATFGEHTVDFKAPYARVTMRQAIIDFT
                     GFDIKGKSEDELRQAAKSMGIEVNETMGKGKLIDEIFGEKCEGNYIQPTFITDYPKEM
                     SPLCKSHRNDPELTERFELMVCGKEIANAYSELNDPIDQRERFEEQVRLAERGDDEAG
                     QFIDQDFLRALEYGMPPTSGLGIGMDRLIMFLTNNPSIQEVLFFPQMRPEVKKVALSE
                     NEKIIFSILEKAKEIDLNELKSQAGLSNKQWDISIKALTKMGVASVTKENDLLTVRLK
                     "
     gene            2477..3868
                     /locus_tag="PKN22_04230"
     CDS             2477..3868
                     /locus_tag="PKN22_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNS41943.1"
                     /translation="MRTKYIDLIDQTFDFPQDEFSLIEDRLLFHGIDLMRLVEKYGTP
                     LKFNYLPQISHNIQRAKGWFRETMNNIGYQGNYHYSYCTKSSHFSFVLDEVMKNDVHI
                     ETSSAFDIDIVKKLQEKGKLKDGQFVICNGFKPKHYIENIANLIEEDKLRVIPIVDNT
                     QEVQDLLARTDKDINIGIRIASEEDPKFEFYTSRLGIRYREIVPFYKAILENNPRVKV
                     KMLHFFINTGITDNAYYWNELTKCLRVYCDLKKVCPTLDSLNIGGGFPIKQSLAFDYD
                     YAYMVREILMQVKKVCSDNEIPEPNIFSEFGSFTVGESGGAIFRVLHQKQQNDREKWN
                     MIDSSFMTNLPDTWAINKRFIMLPINRWNDDYERVLLGGLTCDSDDYYNSEQHANAIY
                     LPRFDRNNPLYIGFFNTGAYQETVGGFGGLKHCLIPEPKHVLIQRDEDGKINTEIFSE
                     EQTANDFLKILGY"
     gene            3931..4305
                     /locus_tag="PKN22_04235"
     CDS             3931..4305
                     /locus_tag="PKN22_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41944.1"
                     /translation="MKLKQVYIKPGGYFSSVINEWATTNNVETIEASLAEGFEENIDG
                     LVIFNQNSDESKEILDIRKIFDKKLKPVHRIDINGTLMVGVSNFSLWLERNACGSVLF
                     LGDDVLKENANLSRYLESFKVQ"
     gene            4372..4896
                     /locus_tag="PKN22_04240"
     CDS             4372..4896
                     /locus_tag="PKN22_04240"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNS41945.1"
                     /translation="MKSLFLALVSLFLTIGIYSCASKKNTTTDVVKTETANNPSSGDT
                     LFASLQRGYCFGRCPVYTLEIYKSGYAVYEGKANVEMLGKYSTRFTKEQMNSLVKVAN
                     EINYASLEDKYDGNITDIPSHTTSIVLNGKRKQVMRRYDYPRSILEFENQFDTLIKEA
                     KWTLLEKYIDPNNR"
     gene            4911..5672
                     /gene="surE"
                     /locus_tag="PKN22_04245"
     CDS             4911..5672
                     /gene="surE"
                     /locus_tag="PKN22_04245"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNS41946.1"
                     /translation="MSKPLILVTNDDGINAKGIETLIRTMQQYGDVVVVAPDSAQSGM
                     GHAITVNHPLRIHRSKLFHDVEAYSTTGTPVDCVKIGIYEILNRKPDLLVSGINHGAN
                     FSTNVLYSGTMSAAVEGAMENIPSIGFSLCDFSADADFTGAKKVVEEVTEMVLRNDFP
                     PHICLNVNIPKVNAEEIKGIQVCKQAAAFWDDRLEKRQDAMGNDYYWLTGKFQEIEVE
                     KNTDLYYIKHNYATIVPTQFDMTAYNVIDELKNWK"
     gene            5672..5983
                     /locus_tag="PKN22_04250"
     CDS             5672..5983
                     /locus_tag="PKN22_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41947.1"
                     /translation="MNLRNWSKEHTKGLIVGILTIAVSIFIVLFILSAINGVPFSESY
                     NRFTYKHDYIAKVISLAAIGNLPWFHFASLRTSNWAFGQGIIIATVLDLLVMIFFKFI
                     I"
     gene            5983..>6320
                     /locus_tag="PKN22_04255"
     CDS             5983..>6320
                     /locus_tag="PKN22_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNS41948.1"
                     /translation="MQKQKFYVIAGEASGDLHASNLMRAMLQENPNCDFRFWGGDEML
                     KVQNNCVKHIKDLAFMGFVEVLLNLKTILGNIKFCKNDILNYQPDTLILVDYPGFNLR
                     IAEWAHQQNI"
BASE COUNT         2111 a         1098 c         1193 g         1918 t
ORIGIN      
        1 actcgaaccc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattctcgc
       61 aaattatatt tcaattgccg tacatcgtgt tgtaacgggc gacaaatgta taaagttttt
      121 ttgaaactcc acacatcttt ttagaaaaaa ataaaatatt tttagcaaaa aggaattttg
      181 tcaattcgtt tttgatgtct tccgccctca aaaggcgtgt ctaaaaagtt gtttaccatt
      241 tcaatagccg tttccgtgct cacaaatctt gcaggcatag caataatgtt ggcatcattg
      301 tgctctcttg ctaattttga aatttctggg gtccaacaca atgctgagcg aattccttgg
      361 tgcttgttaa ccgtcatgtt aataccgttt ccacttccac aaataacaat ccccaacatg
      421 cctttgtttt gttccaccat ctctgcaaca ggatgtccat aatcagggta gtcaatactc
      481 tcttcagaat agcaaccaaa atcctttact tcataacctc ttgattgaag gtgttttatt
      541 atctcttgtt ttaactggaa acctgcgtga tcgcaaccta ttggaattat catacttctt
      601 gttttactac cgcaaaatta gtgaaatcat tcaaaaaaaa attactattt gataaattcc
      661 tgtatttcct gttaaatata ttttggaata tgtgtatttt acagtctatt tacctaaatt
      721 tgctgtcccc aattaaatta tagagaaaaa tatgagtttt attgagttgt ccgagcaaga
      781 attacagcgt agagaaacat tgaatcaaat ccgtcaaatg ggtattgatc cctatcctgc
      841 agctttgtat cctgttgatg attatgctaa aaatataaaa gaaaattttg aagataaaaa
      901 gcaggtttgc attgcaggta gaatgttgag ccaacgaatt atggggaagg cctcttttgt
      961 ggagttgcaa gactcaacaa atagaattca agtgtatttt aaccgtgatg aaatttgtcc
     1021 gggcgatgat aaatcactct acaacgattt gtttaaacgt tttttagatc ttggcgattt
     1081 tgtaggtgta aaaggttacg tgtttaaaac acaagttggc gagatttctg tgcatgtaca
     1141 agaatttaca ttgctaagta aatcacttaa accacttcct ttgccaaaaa ttgatgccga
     1201 aggaaaagtt tacgatgcac taacagatcc agaaacacgc tatcgtcaac gatatgctga
     1261 tttagtggtt aacccacacg taaaagaagt tttcgtaaaa cgtacaaaat tattcaacgc
     1321 catgcgccaa ttctttaatg atgcaggtta tatggaggtt gaaacaccaa tattgcaatc
     1381 aattcctggt ggagctgctg cacgaccgtt tatcacacat cacaatgcgt tggatattcc
     1441 tctttatatg cgtattgcaa atgagttata tttaaaacga ctaattgttg gtggatttga
     1501 tggcgtgtat gaattttcca aaaacttccg caacgaagga atggatagaa cacacaatcc
     1561 cgaatttaca gcaatggaaa tctatgtggc ctacaaagat tacaactgga tgatggattt
     1621 tacagaacgc ttgttggaac actgtgcaat ggcggtaaac ggaacaacaa aagcaacttt
     1681 tggtgaacat acggttgatt ttaaagcacc ttatgcacgt gttaccatgc gtcaagcaat
     1741 tatcgatttc acaggatttg atattaaagg taaatccgaa gacgaattgc gccaagctgc
     1801 taagtcaatg ggaattgaag ttaatgaaac catgggtaaa ggcaagttga ttgatgaaat
     1861 ttttggagaa aaatgtgaag gcaactacat tcaaccaaca ttcattaccg attaccctaa
     1921 agaaatgtcg cctttgtgta aatcacaccg aaacgatcca gaattaacag aacgttttga
     1981 gttgatggtg tgtggaaaag aaattgctaa tgcttattca gaactaaacg atccaattga
     2041 tcagcgtgaa cgctttgaag aacaagtgag attggcagaa agaggtgatg atgaggcagg
     2101 acagtttatt gaccaagatt tcttgcgtgc attggaatat ggaatgccac caacatctgg
     2161 tttaggaatc ggaatggacc gcttaatcat gtttttaaca aacaacccat caatccaaga
     2221 agtgttgttc tttccacaaa tgcgaccaga agtaaaaaaa gtggcgcttt ctgaaaatga
     2281 aaaaattatc ttctctattt tagagaaagc taaagaaata gatttgaatg agctaaaatc
     2341 acaagctggg ttaagtaaca agcaatggga tattagcatc aaagccttga caaaaatggg
     2401 agtggcttcc gttactaaag aaaatgattt gttaaccgtt cgtttgaaat aacgagtttt
     2461 tttattgatt ggtggaatga gaacaaagta cattgattta attgatcaaa cttttgattt
     2521 tcctcaagat gaatttagct tgattgaaga tcgcttattg tttcatggta tcgacttaat
     2581 gcgtttggtt gaaaaatacg gaactccgtt aaaattcaat taccttccgc aaatttcgca
     2641 caacattcaa cgtgccaaag gatggtttcg tgaaacgatg aataacattg gttatcaagg
     2701 taattatcac tattcctatt gtaccaaaag ttcacatttc tcttttgtgt tggatgaagt
     2761 aatgaaaaac gatgtgcaca tcgaaacatc atctgctttt gatattgata ttgttaagaa
     2821 attacaagaa aaagggaagc taaaagacgg tcaattcgtt atttgcaacg ggtttaagcc
     2881 aaaacattat atagaaaaca ttgccaatct tatcgaagaa gataaattgc gcgttattcc
     2941 aattgttgat aatacacaag aagtacaaga tttattggca agaaccgata aagacataaa
     3001 cattggtatt cgtattgctt ccgaagaaga tccaaaattt gagttctata cctcgcgttt
     3061 aggaattcgc taccgtgaaa tagttccttt ctacaaagca attttggaga ataacccacg
     3121 tgtaaaagtg aaaatgttgc actttttcat caacacaggg attacagata atgcctatta
     3181 ttggaacgag ttaactaaat gtttgcgcgt ttattgcgat ttgaaaaaag tatgtccaac
     3241 actcgattca cttaacattg gtggaggttt tccaatcaaa caatcgcttg catttgatta
     3301 cgattacgcc tatatggtgc gcgaaatttt gatgcaggtc aaaaaagtat gttccgataa
     3361 cgaaattcca gaaccaaaca tcttctcaga atttggatcc tttacagttg gtgaaagtgg
     3421 aggagcaatt tttcgtgtgt tgcaccaaaa acaacaaaat gaccgtgaga aatggaacat
     3481 gattgactct tcgttcatga ccaatctgcc tgatacttgg gcaattaaca agcgttttat
     3541 tatgttgcca ataaaccgtt ggaacgatga ttatgaacga gttttgctcg gtggtttaac
     3601 atgtgattca gacgattatt acaattctga acaacacgcc aatgccattt atttgcctcg
     3661 ttttgataga aataacccac tttatatagg tttcttcaat acaggtgctt accaagaaac
     3721 ggtaggaggt tttggagggt taaaacactg cttgattccc gaaccaaagc acgtactcat
     3781 tcagcgcgat gaagatggaa aaatcaatac agaaatcttt agcgaagaac aaacagccaa
     3841 cgatttttta aaaattttag gatattaatt tatttttgaa atcaattcat tacttttgca
     3901 ccccgattca aatgtgaatc cactagaaat atgaagctta aacaagtata tatcaagcct
     3961 ggaggctatt ttagctcggt tattaacgaa tgggcaacaa caaataatgt tgaaaccatt
     4021 gaagcatctt tagccgaagg atttgaagaa aatattgacg gtttggtgat ttttaatcaa
     4081 aactctgacg aaagcaaaga aatccttgac atcagaaaaa tatttgataa aaaattaaaa
     4141 ccagtacaca gaattgatat caacggaaca ttgatggtgg gagtttctaa cttctcactt
     4201 tggttagaaa gaaatgcttg cggtagcgta ttgtttctag gtgatgatgt gttgaaagaa
     4261 aacgccaatc tttcccgtta cctcgaatca tttaaagttc aataatttta ctttctatac
     4321 ttttggttgg atttttgatt atatatttgt tgtaatcaaa aattgaataa aatgaaatcc
     4381 ctgtttttag cacttgtaag cttattccta accattggaa tctattcttg tgcgtccaaa
     4441 aaaaatacaa ctacagatgt tgttaaaaca gaaacagcta acaatccttc ttccggagat
     4501 acattatttg cttcattgca aagaggctac tgttttggta gatgccctgt ttatacccta
     4561 gaaatctaca aatcgggata tgctgtatat gaaggaaaag ccaatgtgga aatgttaggg
     4621 aaatacagca cacgttttac taaagaacaa atgaactcat tggttaaagt tgccaacgaa
     4681 atcaattatg cttcacttga agataaatac gacggcaata ttaccgatat accaagtcat
     4741 accacctcca ttgtattaaa cggaaaacgt aaacaagtga tgcgacgata cgattatccg
     4801 cgctctatcc tcgaatttga aaaccaattt gatacactca tcaaggaggc aaagtggaca
     4861 cttttagaaa aatatattga cccaaacaat cgttaataca acaaaaaaac atgtcaaaac
     4921 cgcttatttt agtaaccaac gacgacggaa ttaacgcaaa aggtatagaa accttaatca
     4981 gaaccatgca gcaatatggc gatgtggtag tggtggcacc agattctgca caatcaggta
     5041 tgggacacgc aattactgtt aatcatccgt tgagaattca tcgctctaaa ttgtttcacg
     5101 atgttgaagc gtattctact acaggtacgc ccgttgattg tgtgaaaata ggtatttacg
     5161 aaattttaaa cagaaaaccc gacttgctag tttctggtat caatcatgga gccaattttt
     5221 caaccaatgt gttgtattct ggaaccatgt cggcagccgt tgaaggagca atggagaata
     5281 ttccatccat cggtttttct ctttgcgatt tttctgccga tgccgatttc acaggcgcaa
     5341 aaaaagtggt agaagaagta accgaaatgg tgttgcgcaa tgattttcct ccgcacattt
     5401 gcttaaatgt caatattccg aaggtgaatg ctgaagaaat taaaggcata caagtgtgta
     5461 aacaagccgc tgctttttgg gatgatcgct tggaaaaacg acaagatgcg atggggaacg
     5521 attattactg gttgacaggt aaattccaag aaattgaagt agaaaaaaat accgacttgt
     5581 attacatcaa acataattat gctactatcg tacctacaca atttgatatg acagcttata
     5641 atgtgattga cgaacttaaa aattggaaat aatgaatctt agaaactggt caaaagaaca
     5701 tacaaaagga ttaatagtgg ggattctcac catcgctgtc agcattttta ttgtattatt
     5761 tatcctttca gcaataaatg gcgtaccttt ttctgaaagt tataatcgct ttacatataa
     5821 gcacgattat attgccaaag ttatttcgtt agcagccatt ggaaatctgc cttggtttca
     5881 ttttgcttcg ttgagaacga gtaattgggc atttggtcaa ggtatcatca ttgcaactgt
     5941 tttggattta ttggtaatga tattctttaa attcattatc tgatgcaaaa acaaaaattt
     6001 tatgtcatag ctggagaagc ttcaggagat ttacatgcaa gcaacctaat gcgtgccatg
     6061 ttgcaagaaa atcccaattg cgattttaga ttttggggcg gagatgaaat gctaaaagtg
     6121 caaaacaatt gcgttaagca catcaaagat cttgcattca tggggtttgt ggaagtgttg
     6181 ctcaacctta aaaccattct tggcaacata aaattttgta aaaacgatat tctaaattac
     6241 caacccgata cgctgatttt ggtagattat cccggtttca acttgcgaat agcagaatgg
     6301 gcacaccaac aaaacatcaa
//
LOCUS       DAOQVQ010000094         6291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13691_length_6291_cov_4.347659, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000094 DAOQVQ010000000
VERSION     DAOQVQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13691_length_6291_cov_4.347659"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKN22_04260"
     CDS             <1..754
                     /locus_tag="PKN22_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS41949.1"
                     /translation="NDLQEKLGRILLISDAAHSFGATMNGKKAGALADISTFSFHAVK
                     NLTTAEGGAICFNLPASFDAEAIYKHFCTLILHGQNKDALAKTQKGNWRYDVEEPGFK
                     CNMTDLQAAIGLIELDRYQENLDRRQHIFDRYNAGFANEDWAIAPKFKDENRETCYHL
                     YLLRIKGITEQQRDAVMQQIFDRDVSVNVHFLPLPTLTAYKKRGYKMEDYPETWNKYA
                     NEISLPVYFDLTDEQVQTVIQAVKEAYKAVVK"
     gene            790..1338
                     /locus_tag="PKN22_04265"
     CDS             790..1338
                     /locus_tag="PKN22_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41950.1"
                     /translation="MKNTFHWLFVVVIIAFTAVSCKKDKYASFEVTLSSDKVVAKKAT
                     VRIKEVWLNYATKKSNSEWMKLEVDTVSYDLARLYNGQVDTLFIPEVKVDNITTLMKF
                     RVVVAADKHSVITPADDTLEMTLDPTLTDGIKASVNKSLENLKTYTVQLKFQPDTVAM
                     SSSTPVFKSSIALKGFGIKGNI"
     gene            1458..2930
                     /locus_tag="PKN22_04270"
     CDS             1458..2930
                     /locus_tag="PKN22_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNS41951.1"
                     /translation="MKKLVLGIIVGCMSFGSMAQQKIEFVEYDLPNGIHVILHQEKST
                     PIVAVSVLYHVGSKNEKPDRTGFAHFFEHLLFEGSKNIGRGEYSEYVEKNGGALNANT
                     SMDRTFYFELLPSNQLELGLWLESERMLHAKVDSVGIETQRSVVKEEKRQRVDNQPYA
                     TFIKELFDRLFPNSTYGWQPIGSMEHLDAAQEEDYVNFYQTFYVPSNATLSIAGDIDI
                     EQTKKWIDKYFKAIPKGQAINLYRDYLSLKDDKFKTKYGVEKSIFDSKDFFASKNAKA
                     KALIAEYSNKPTSIERPAKPSQKPNNAEVVTVYDNIQLPALFVGYVLPEQRNEDMYAL
                     EFLNSVLSGSSSSRINKDVVEKKQLASMAFSFAYGLENAGAEIIAAIAQKDVKLDAIL
                     AEIDAQIEKVQNELITQEEFERVRNQFENQAISSYSTISGISEALADAHVYRGSANYV
                     NVELEKYMKVTREDIQRVAKKYFTKDARVILHYLPKEESK"
     gene            2940..5027
                     /locus_tag="PKN22_04275"
     CDS             2940..5027
                     /locus_tag="PKN22_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNS41952.1"
                     /translation="MKKLIIALGLISPALLFGQLDRSVVPAAAPAKAISIADPVVFKT
                     ANGITVILSENHKIPKVTFNLSVGNGERLEGKKAGVSSLAGDLILSGTTKRSKDEIDA
                     KKDYIGASIDAGAKSMSLSCLTKHMDTGLDIFSDILLNANFPQSEFERIVKQAESSLM
                     SVKSSPESMGQNALMTSLFTGHPYGEVMTEATLKNITRDDVVYYYKQTFVPEGSYLVI
                     VGDITKAKAEEVVNKYFSSWTGKLQPKVEYPSAKNLKGNRVIFVKKQGAVQSYIQVAF
                     PVGIKIGDANYIPTSLTNHIFGGSGFGTRLMQNLREDKAYTYGAYSNLSVQQQGAYVS
                     AAGSFRNDVTDSAIVQLLSELNKITNSYVTAEELSLAKAVLTGRFARSLESPSTIARF
                     ALEIERNGLDKNYYKDYLKNLDAVDKEKVLDIAQKFYTDKNCYIIVVGNESVLEKLKQ
                     FDADGNIEVVDAFGEPVQDMKKADITADELFKKYSLALTASKTVKERDKKLKKIKSVR
                     QEVEMSMSQVPFPLKSVRFWAAPNSEVMTIEAQGMMVQKTYFDGTTGYNSNMQTGKKD
                     LTAEEIAAKNKSTGLIPEINYTKSGMKYELLGIENQNGTDMYVVKLNDGESESYDYYN
                     TKDFSKIKTVTISKAGEEEEGPSEVTMTYSNYKAVGGVLFPHKIVMSAGAMTFNGEVK
                     LIEINKGALKDFK"
     gene            complement(5210..5449)
                     /locus_tag="PKN22_04280"
     CDS             complement(5210..5449)
                     /locus_tag="PKN22_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNS41953.1"
                     /translation="MKRQSISFTEPNDEWLKQQIATKEYTSKSEIINDLIRKVRTQQQ
                     EFDWIRTKIEKAERSGFTSDNQAQILQQSKDLLNE"
     gene            5617..5826
                     /locus_tag="PKN22_04285"
     CDS             5617..5826
                     /locus_tag="PKN22_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41954.1"
                     /translation="MKTYNKLMVTLWLVIAILITLFVTYKTIMEGVNRWGFMYVFAAM
                     AFMMYFLRRYMYRKVEKGMNNGKEK"
     gene            complement(5932..>6291)
                     /locus_tag="PKN22_04290"
     CDS             complement(5932..>6291)
                     /locus_tag="PKN22_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNS41955.1"
                     /translation="GYSFNLDGTNIYMTLAALFIAQACNIHLEFDHLMVLLLVAILSS
                     KGAAGVTGAGFITLAATLTVVPEIPVAGMTLILGIDKFMSECRALVNVIGNGVACVVV
                     SNWEKHLDKEQLKKTLG"
BASE COUNT         2162 a         1019 c         1221 g         1889 t
ORIGIN      
        1 caatgacttg caagaaaaat taggtagaat tttattgatt tcggatgcag ctcatagttt
       61 tggagcaacc atgaatggga aaaaagcggg tgctcttgcc gatattagca ctttttcatt
      121 tcacgcagta aaaaacctaa caacagctga gggtggggca atttgcttta atttaccagc
      181 ttcttttgat gccgaggcaa tttacaaaca tttttgtacg ctcattttgc acggtcaaaa
      241 caaagatgca ttggcaaaaa cacaaaaagg aaattggcgc tacgatgtgg aagaaccagg
      301 ttttaaatgc aacatgaccg atttgcaagc cgctattgga ttgattgaat tggatcgcta
      361 ccaagaaaat ttagacagaa gacaacatat ttttgatcgc tacaatgctg gttttgccaa
      421 cgaagattgg gcaattgcac caaaattcaa agacgaaaat cgtgaaacat gttaccactt
      481 gtatttgttg cgcatcaaag ggataactga gcaacagcgt gatgccgtga tgcaacaaat
      541 ttttgatcgt gatgtttctg taaacgtgca tttcttgcct ttgccaacgt taacggctta
      601 caaaaaacgc gggtataaaa tggaagatta tccagaaaca tggaacaaat atgccaatga
      661 aatttcgttg cctgtttact ttgatttaac cgacgagcaa gtgcaaacag taatccaagc
      721 tgtgaaagaa gcgtataaag ctgttgtaaa ataataattg tatctttgct agaaccaaac
      781 gcacacaata tgaaaaatac atttcactgg ttattcgttg ttgtaatcat tgcttttaca
      841 gcggtttctt gtaaaaaaga caaatatgct tcgtttgaag ttaccttgtc atcggataaa
      901 gtagtggcta aaaaagcaac tgtaagaatc aaagaagtgt ggttgaatta cgctaccaaa
      961 aaatccaatt cagagtggat gaaattagag gtggacactg tttcgtatga tttggcacgt
     1021 ttgtataatg ggcaagtgga tacattgttc attccagaag tgaaagttga caacattaca
     1081 accttgatga aatttcgtgt ggtggttgct gcagacaagc attcggtaat tacaccagca
     1141 gacgatacgc ttgaaatgac gttggatcca acattaaccg atggcatcaa agcaagtgtc
     1201 aataaatcgt tagaaaattt aaaaacatat acggtacaat tgaaatttca acccgatacg
     1261 gtggctatga gcagttcaac gcctgttttt aaatcatcca ttgcactaaa aggatttgga
     1321 attaaaggaa atatctaaaa aaaccttttt ctgattttca tcctttgaat aaaccgttta
     1381 cccatatttt caaccattga agtaattcat tagcattttt acaaaagatt gatttaattt
     1441 gtaaaaaaat gtaacgaatg aaaaaactag ttttaggaat catcgtaggt tgcatgtcct
     1501 tcggttcaat ggctcaacaa aaaattgaat tcgtagaata cgacttacca aatgggattc
     1561 acgtaatcct acaccaagaa aaatctacgc ctatcgtagc agtttctgta ctttatcatg
     1621 tgggttcaaa gaatgaaaaa ccagacagaa caggttttgc acacttcttt gagcatttat
     1681 tgtttgaagg ttcaaaaaac attggtcgcg gtgaatacag cgaatatgtg gagaaaaacg
     1741 gaggtgcatt gaacgcaaat acgtcaatgg acagaacgtt ttattttgaa ttattgcctt
     1801 ctaatcagtt ggaattaggt ttgtggttgg aatctgagcg tatgttgcac gcaaaagtgg
     1861 acagcgtagg aattgaaaca cagcgcagtg tagtaaaaga agaaaaacgt cagcgtgttg
     1921 ataatcagcc gtatgcaacg tttatcaaag aattgtttga ccgtttgttt cctaattcaa
     1981 cttacggatg gcaaccaatt ggtagcatgg agcacttaga tgcagcgcaa gaagaggatt
     2041 atgtgaattt ttaccaaaca ttttatgttc cttcaaatgc aacattgtct attgcaggtg
     2101 atatcgacat cgaacaaact aaaaaatgga ttgacaaata tttcaaagct attccaaaag
     2161 gacaagcaat caacttgtat agagattact tgagtttgaa agatgataaa ttcaaaacga
     2221 aatacggtgt tgaaaaatca atctttgatt ctaaagattt ctttgcttca aaaaatgcaa
     2281 aagcaaaagc gttgattgca gaatatagca ataagccaac atcaattgaa cgtccggcaa
     2341 aaccttcaca aaagccaaac aatgctgagg tggtaaccgt ttacgataac attcaattgc
     2401 ctgctttgtt tgtagggtat gtattgccag agcaacgcaa tgaagacatg tatgctttag
     2461 aatttttaaa ttctgtgctt tctggaagtt ctagttcacg catcaataaa gatgtggtag
     2521 aaaagaaaca attggcttct atggcatttt cttttgctta tggtttagag aatgcaggag
     2581 ctgaaattat tgcagccatt gcacaaaaag acgtgaagtt ggatgcaatt ttagctgaaa
     2641 ttgatgcaca aattgaaaaa gttcaaaacg aattgattac acaagaagag tttgagcgtg
     2701 taagaaatca atttgaaaac caagcaattt cttcgtattc aacaatttct ggtatcagcg
     2761 aagcgttggc agatgcacac gtatatcgtg gaagtgcaaa ttatgtaaac gttgaattgg
     2821 aaaaatacat gaaagttact cgcgaagata ttcaacgtgt agctaaaaaa tattttacaa
     2881 aagatgcacg tgtaatttta cattatttac caaaagaaga atctaaataa ttgacgaaaa
     2941 tgaaaaaatt aataatagca ttaggactta tttcaccggc tttacttttc ggtcaattgg
     3001 atcgttctgt tgtgcctgct gctgcacctg caaaggcgat cagtattgca gatccggtgg
     3061 tttttaaaac agcaaacgga ataacagtta ttctttcaga aaaccataaa attccaaaag
     3121 ttacctttaa cttatcagtt ggaaatggcg aacgtttgga aggtaaaaaa gcaggtgttt
     3181 ctagccttgc gggagattta attttgagcg gaacaacaaa acgttcaaaa gatgaaattg
     3241 atgcaaagaa agattacatt ggtgcaagca ttgacgctgg tgcaaaaagc atgagcttga
     3301 gttgtttgac aaaacacatg gacactggtt tggatatctt ttcagacatc ttgttgaatg
     3361 caaatttccc tcaaagcgag tttgaacgta tcgtaaaaca agcagaaagt agtttgatgt
     3421 cagttaaatc tagcccagaa tctatgggac aaaatgcatt gatgacatct ttgtttacag
     3481 ggcaccctta tggcgaagta atgacagaag caacgttgaa aaacattaca agagacgatg
     3541 tggtgtatta ctacaaacaa acatttgtgc cagaaggtag ttacttggta attgtgggtg
     3601 acattacaaa agcaaaagcg gaagaagtag tgaacaaata tttctcttct tggacaggta
     3661 agttacaacc aaaagtagaa tacccatcgg ctaaaaactt gaaaggtaac cgtgtgattt
     3721 ttgttaaaaa acaaggtgct gttcaatcgt atattcaagt tgctttccca gttggaatca
     3781 aaattggaga tgcaaattac attcctacca gtttaaccaa ccacattttt ggtggaagcg
     3841 ggtttggaac acgtttgatg caaaatttgc gtgaagataa ggcgtatacc tatggagctt
     3901 attcaaattt aagtgtacaa caacaagggg cttatgtttc agctgcaggt agctttagaa
     3961 atgatgttac tgatagtgca attgttcaat tgttaagtga gttgaacaaa attaccaaca
     4021 gctatgttac ggcagaagaa ttgagtcttg caaaagcggt gttaactggt cgttttgcac
     4081 gttctttgga aagtccatca acaattgctc gttttgcatt agaaattgag cgcaatggtt
     4141 tggataaaaa ctattacaaa gattatttga aaaatttgga tgcggttgac aaagaaaaag
     4201 tgttggacat tgctcaaaaa ttctatacag ataaaaattg ctacatcatt gttgttggaa
     4261 acgagtcagt tttagaaaaa ttaaaacaat ttgatgcgga tggcaacatt gaagtggtag
     4321 atgcttttgg tgagccagtt caagacatga aaaaagcgga catcacagca gatgaattgt
     4381 ttaaaaaata ttctttggca cttactgctt ctaaaacagt aaaagaaaga gataaaaaat
     4441 tgaaaaaaat caaatcggta cgtcaagaag ttgaaatgtc aatgtcgcaa gtacctttcc
     4501 cattaaaatc agttcgtttt tgggcagctc caaacagtga agtaatgacc attgaagcac
     4561 aaggaatgat ggttcaaaaa acctattttg atggaactac aggttacaat tccaatatgc
     4621 aaactggtaa aaaagactta actgcagaag aaattgcagc gaagaacaaa tctactggtt
     4681 tgattccaga aatcaactat acaaaatcag gaatgaaata tgagttgttg ggaattgaaa
     4741 accaaaatgg aacagatatg tatgtagtaa aattaaacga tggtgaaagc gaatcgtatg
     4801 attactacaa tacaaaagat ttcagcaaaa tcaaaacagt cactatcagc aaagcaggtg
     4861 aggaagaaga aggtccaagt gaagttacaa tgacttattc taactacaaa gcagttggtg
     4921 gcgtattgtt tccacacaaa attgtaatgt ctgcaggcgc aatgacattc aacggagaag
     4981 tgaaattaat tgaaatcaac aaaggagcat tgaaagattt taaataatct tttatctact
     5041 tttagtagca aaagcggcgc agtgaatgtg tcgcttttgt tgtttttgga ggtttttata
     5101 atgaaggagt tttgatgtta tctaaaatct tgccttccta taattgtcat gatttctact
     5161 gtattctcta cgattcgata attgatagaa tcaacaccac attctgtaat tattcattca
     5221 acaaatcttt tgattgctgt aatatttgtg cttgattatc tgaagtaaat ccacttcttt
     5281 cagctttttc aattttcgtg cgaatccaat caaattcttg ttgttgtgtt ctaacttttc
     5341 taattaaatc gttgataatt tcacttttac tagtgtattc tttagtggca atttgttgtt
     5401 ttaaccactc atcattaggc tctgtaaaag aaatgctttg tcttttcatt tttattaaaa
     5461 tatggtgtaa atatacacca aaaatgcatc aaacacactt ttttttagaa tttactttgt
     5521 ttaacaagtg gagtgctcta tttctaatat gcattttttt cttcaaagtt ggacttcccc
     5581 aatattttga agtatcttta cccaaaaata aaacacatga agacatacaa taaattaatg
     5641 gttacgcttt ggttggttat tgctattttg attacattgt ttgtaaccta caaaaccatc
     5701 atggaaggag taaaccgttg gggatttatg tatgtgtttg cagcaatggc ctttatgatg
     5761 tattttttac gccgttacat gtaccgcaaa gttgaaaaag gcatgaacaa cggaaaagaa
     5821 aaatagcatt tctcactcat ttttcttatt ttccaataaa agtttgatgt tttcattcaa
     5881 aggttgatgt gttttaaaaa aacaaatcaa caaaaagtat ttttacttcc tctatcccaa
     5941 cgttttcttc agctgttctt tgtctaaatg tttttcccaa tttgaaacaa ctacgcatgc
     6001 cacgccgttg ccaattacat tcaccaaagc acggcattcc gacatgaatt tgtcaattcc
     6061 caatatcaag gtcataccgg ctactggaat ttcgggcaca acggtcaatg ttgcagcaag
     6121 ggttataaat ccagctcctg ttactccagc cgcacctttt gaagataaaa ttgctaccaa
     6181 aagcaacacc atcaaatggt caaattctaa gtgaatgttg cacgcttgcg caatgaacaa
     6241 tgcggctaaa gtcatgtaga tattggtgcc atctaagttg aatgaatacc c
//
LOCUS       DAOQVQ010000095         6247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13836_length_6247_cov_3.820090, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000095 DAOQVQ010000000
VERSION     DAOQVQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13836_length_6247_cov_3.820090"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PKN22_04295"
     CDS             <1..685
                     /locus_tag="PKN22_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41956.1"
                     /translation="GDIVNPNAEILITLKDDNPYMLMNEPADTSLFGIYIKDPNGNLK
                     RIPFVDGQGNTVMQWIPAESNYKKFKIIYPAAFTMDGTYQLLVQGADKSGNLSGDYQY
                     KVNFEVIRASSITNMMNYPNPFSTSTRFVFTLTGSQVPDDIIIQIMTVSGKVVREITE
                     DELGRIYIGKNVTEYAWDGRDEFGDQLANGVYLYTVKAKINGENIEHRESGADKYFKK
                     SFGKMYLMR"
     gene            complement(743..1402)
                     /locus_tag="PKN22_04300"
     CDS             complement(743..1402)
                     /locus_tag="PKN22_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HNS41957.1"
                     /translation="MALDKNGIAKRIAQELRDGWYVNLGIGIPTLVANYIPEGIEVEF
                     QSENGILGMGPFPFEGEENADLINAGKQTITVKKGAVFFDSATSFAMIRGQHVQLTVL
                     GAMEVSQNGDIASWKIPGKMVKGMGGAMDLVASAENIIVAMMHTNKAGESKILKECTL
                     PLTGVGCVKKIVTDLAILEVKDGKFHLIERAPGVSVEEIVAKTEGDLVVPDFVPEMQL
                     D"
     gene            complement(1422..2120)
                     /locus_tag="PKN22_04305"
     CDS             complement(1422..2120)
                     /locus_tag="PKN22_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNS41958.1"
                     /translation="MNKVVKNVEEALKGIEDGMTLMVGGFGLCGIPENSIAELVKKGT
                     KNLTCISNNAGVDDFGLGLLLQGKQIKKMVASYVGENAEFERQMLSGELEVDLIPQGS
                     LAERCRAGGAGIPAFYTPTGYGTEVAEGKEVRVYNGKPHILEEALTADFAIVKAWKGD
                     TEGNLIYKETARNFNPMMAMAGKITIAEVEELVPAGELDPNFIHTPGIFVQRIFQGEK
                     FEKRIEQRTTRKRD"
     gene            complement(2139..4625)
                     /locus_tag="PKN22_04310"
     CDS             complement(2139..4625)
                     /locus_tag="PKN22_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNS41959.1"
                     /translation="MSAKKDTENFSEKTTKRLYWLLWLGAMTPIIIIAFLLLIQSEDD
                     LPSIEMLDNPPEMLASIIYADDAQTELGRYWQVNRTNVAYKDISPNVINALISTEDER
                     FMNHSGVDFKGLSRAILNLGSKGGASTITQQLAKLLFTLQKREKEREARISGNILPVA
                     GKLSRINEKAQENIIAVRLEKRFTKEEIITMYLNQFDFLYNAVGIENAAKVYFNKKPS
                     ELSKSEAACLVGMVKNPSLYNPRTYQVRDYRGRVAANLEISPEQVTKAQIKEERQKDS
                     LLIHQRRNQVLMQWLKNSESGNDAISEKLTRAEYDKLKNEPIIVDYQVVDHKEGIAPY
                     YRESLRKELTELFESKDENGELKYKKKDGTSYNIYKDGLRIYTTINYNMQKYAEEALV
                     YHLKKDLQPEFDKNNKRRKRFPFADSYNGVPISDEQIESIMNRARKGSERYISMKEQG
                     LSEKEILASFNKKTPMSVFSWKGDIDTVMTPNDSIRYYKGFLHAGLVSIEPQSGFIKA
                     WVGGADISNFAYDHVKQGKRQVGSTIKPFVYGTALAMGVVKPCTIFEDMPYCVDLQDA
                     NGRIDGRWCPSGDIPIGKTVAWALQNSNNPGTVAVMSLMGGYAGPKTISKLLKELDIH
                     LRPEDEVPSMCLGVMDLSLIEMTAAQAMWANNGIYNRPISVLRIEDRNGNVIYSEEGY
                     SKEVMNENVAYETLKMMKGVVNGGTAGSLRGTWRPWGGLTAPMAGKTGTTQGNADGWY
                     MGLTPELVTGVWVGAEDKQVRFQSMEWGQGGRSALPIFGYYMQKVYKDKEINLTTADF
                     PVPSSYDPTQFSCADTLNTAPQGEEIDIFR"
     gene            complement(4669..5694)
                     /locus_tag="PKN22_04315"
     CDS             complement(4669..5694)
                     /locus_tag="PKN22_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNS41960.1"
                     /translation="MITQAYQLVKKGDATTAFQMNSVQLPSLQSHEVLIKVEAFGLNY
                     ADVMARNGLYREAPPMPCVLGYEVVGEVIEVGASEQQSLIGKRVVGFTRFGGYAQHAI
                     TTIDAISEIGDMPTNNALALATQGVTAYYMANYISPLRIGEKVLIHAAAGGVGTLLIQ
                     LAKLSGAVVVAKVSSAEKEAKCKELGADFTVNYKKEDYVEFINRHIELFRLDASFNPI
                     GGSTFKKDKQVLGFGSRLFLFGGSQLSEGKMGILSQLNFLRKMGVLIPAFQMMQSKSI
                     IGVNMLKIADNKPEVLKHCLNAVVELYKLGKIKTENGGDYQHTALSEAHDLLESGKSI
                     GKIAIYW"
     gene            complement(5691..>6247)
                     /gene="rsmI"
                     /locus_tag="PKN22_04320"
     CDS             complement(5691..>6247)
                     /gene="rsmI"
                     /locus_tag="PKN22_04320"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNS41961.1"
                     /translation="LLAHLNISKNVFPFHAHNEHNALNNVVQRIQTSELVVLVSDAGT
                     PGISDPGFLLVRECVKNEINIECLPGPTAFVPALVASGFPCDKFVFEGFLPHKKGRQT
                     RLQILAEESRTIVLYESPHRLVKLLGQIEEFMGKERQVCVVRELTKIYEEYQRGTAEE
                     VKQHYEKHEPKGEIVVIIEGKSKE"
BASE COUNT         1796 a         1317 c          978 g         2156 t
ORIGIN      
        1 tggcgatatt gtaaatccaa acgctgaaat tttaattacg ctaaaagacg ataaccctta
       61 tatgttgatg aatgaaccag cagacacatc tttatttggg atttacatca aagatccaaa
      121 tggcaactta aaacgtattc cttttgtgga tgggcaaggc aataccgtta tgcaatggat
      181 accagcggaa agtaattaca agaaatttaa aatcatctat cctgctgcgt ttaccatgga
      241 tggaacttat caattattgg ttcaaggtgc cgataaaagc ggaaatttat cgggagatta
      301 tcaatacaag gtcaattttg aagtaattcg agcttcatcc attaccaata tgatgaacta
      361 tcccaatcca ttctcaacca gtactcgttt tgtgtttact ttaactggtt cgcaagtgcc
      421 ggatgatatc atcatacaaa ttatgaccgt ttctggtaaa gtggtgcgtg aaattacaga
      481 agacgaattg ggtagaattt acattggtaa aaacgttaca gaatatgctt gggatggtag
      541 ggatgaattt ggtgatcaat tggcaaatgg cgtatatctc tacactgtaa aagcaaaaat
      601 taatggagag aatattgaac accgcgagtc gggtgccgat aaatacttta agaaaagttt
      661 tggtaaaatg tatttgatgc gatagtacca attaaacata aaaaaaggct ccaattggag
      721 cctttttatt tataacaaaa tactaatcaa gttgcatttc tggaacaaaa tcaggaacaa
      781 ctaaatcgcc ctctgttttt gcaacaattt cttctaccga aacacctggt gctctttcaa
      841 ttaaatggaa tttaccatct tttacttcta aaattgccaa gtcggttaca atcttcttca
      901 cacatccaac tcctgttaaa ggcaaagtac attcttttaa tatttttgat tcacctgcct
      961 tgttggtgtg catcattgct acaataatgt tttcagcaga agcaaccaaa tccatcgctc
     1021 cacccatacc ttttaccatt tttccaggaa ttttccaact tgctatatcg ccattttgcg
     1081 atacctccat tgcacctaaa actgtaagtt gtacgtgctg accgcgaatc attgcaaacg
     1141 atgttgcaga atcaaaaaat acagcacctt tttttacggt aattgtttgt tttcctgcat
     1201 taattaaatc tgcattttct tctccttcaa acggaaaagg acccattcct aaaattccat
     1261 tttcagattg aaattcaact tcaattcctt ctggaatgta gttggcaacc aatgttggga
     1321 taccaatacc taaattaaca taccaaccat ctcttaattc ttgtgcaatg cgttttgcaa
     1381 ttccattttt atctagtgcc atgacttaaa atctttaaaa attaatctct ttttcttgta
     1441 gtgcgttgct caatgcgttt ttcaaacttt tctccctgga aaatacgttg aacaaaaatt
     1501 ccaggtgtat gaatgaagtt aggatctaat tcaccagcag gtaccaattc ttccacttcg
     1561 gcaattgtaa ttttcccagc cattgccatc atcgggttga aatttcgtgc tgtttcttta
     1621 taaatcaaat tcccttccgt atcacctttc catgctttta cgattgcaaa atcagctgtt
     1681 aatgcttctt ctagaatgtg tggtttacca ttgtaaacac gtacttcttt tccttctgcc
     1741 acctcagttc cataaccggt tggtgtataa aatgctggaa taccggcacc tcctgctctg
     1801 catcgttcag ctaatgaacc ttgtggaatt aaatcaactt ccaattctcc cgacaacatc
     1861 tgacgttcaa actctgcatt ctcacctacg taggaagcaa ccattttttt gatttgtttt
     1921 ccttgcaata aaagccctaa tccaaaatcg tcaacacctg cattgttgga aatacatgtt
     1981 aagttttttg ttcctttttt aaccaattct gcaatagagt tttcaggaat tccacataaa
     2041 ccaaagccac ctaccatcaa ggtcatccca tcttcaatgc cttttaatgc ttcttcaacg
     2101 ttttttacaa ctttattcat atcgttaatt attcaatgtt atctaaatat atcaatttct
     2161 tctccttgtg gtgcagtatt taaagtgtct gcacatgaaa attgcgttgg atcgtaacta
     2221 ctaggtacag ggaaatctgc tgtagttaaa tttatttctt tgtctttata tactttttgc
     2281 atgtaatatc caaatattgg caatgcagaa cgtccacctt gtccccactc catcgattga
     2341 aaacgtactt gcttgtcttc agctcctacc caaactccgg ttaccaattc gggggttaag
     2401 cccatgtacc aaccatcggc atttccttgt gttgtacctg ttttaccagc cattggtgct
     2461 gtcaatcctc cccaaggtcg ccatgttcca cgtaatgaac ctgcagtacc gccatttaca
     2521 actcctttca tcattttcaa ggtttcgtat gcaacatttt cgttcattac ttctttggag
     2581 taaccttcct cactgtaaat cacgtttcca tttctatctt caattcgtaa gactgaaata
     2641 ggtcggttgt atattccatt atttgcccac atagcttgag cagccgtcat ttcaattaat
     2701 gataaatcca taacccccaa acacatggaa ggtacttcat cttcagggcg caaatgaata
     2761 tctaactctt ttaataattt agaaattgtt tttggtccgg catagccccc catcaacgac
     2821 attaccgcca ctgttccagg gttgtttgaa ttttgcaagg cccatgcaac tgtttttcca
     2881 attggaatat ctccacttgg acaccatcta ccatctattc gtccgttggc atcttgcaaa
     2941 tcaacacaat aaggcatgtc ttcaaaaatt gtacacggtt ttactacacc cattgccaat
     3001 gcagttccat aaacaaacgg tttgattgtt gaacctactt gacgttttcc ttgcttcaca
     3061 tggtcgtatg caaaattgga aatatcagct ccacctaccc aagctttgat aaaaccagat
     3121 tgtggctcaa tggaaaccaa ccctgcatgc aaaaatcctt tgtaatagcg aatggaatca
     3181 tttggagtca ttacagtgtc aatatctcct ttccatgaaa aaacagacat tggtgttttt
     3241 ttgttgaacg aagctaatat ttctttttca gataatcctt gttctttcat agagatataa
     3301 cgttcactac ctttgcgtgc acgattcatt atactttcaa tctgctcgtc agaaattgga
     3361 acaccattgt atgaatctgc aaatggaaaa cgttttctgc gcttgttatt tttatcaaat
     3421 tctggttgca aatctttttt taagtggtaa actaacgctt cctctgcata tttttgcatg
     3481 ttgtaattga tggtagtgta gatgcgtaaa ccatctttat aaatgttgta ggatgtgccg
     3541 tctttctttt tgtatttcaa ctcaccattt tcgtctttag actcaaacaa ttctgtaagt
     3601 tcttttctca aggattcacg gtaatatggt gcaattcctt ctttatgatc aactacttga
     3661 tagtcaacaa taattggttc gtttttcaac ttatcgtatt cagcacgagt taatttttct
     3721 gaaatagcat catttccact ttcactgttt tttagccatt gcatcaacac ctgatttcta
     3781 cgttggtgaa tcaacaaact atctttctga cgctcttctt ttatttgagc ttttgttact
     3841 tgttctggtg atatttctaa attagctgct actcgccctc tgtaatctct cacttgatag
     3901 gtacgaggat tatataatga aggattcttc accatcccaa ccaaacaagc tgcttcactt
     3961 tttgacagtt cacttggttt tttattaaaa taaaccttag ctgcattttc aattccaaca
     4021 gcattgtata agaaatcaaa ttgattcaaa tacatggtga taatttcttc ttttgtaaaa
     4081 cgtttttcta aacgaactgc aataatattc tcttgtgcct tttcgttaat acgactcaat
     4141 ttgcctgcaa ctggaagaat atttcctgaa attcttgctt ctctttcttt ctcccttttt
     4201 tgaagcgtaa acaataattt tgctaactgc tgtgtgatag tagatgcacc acctttactt
     4261 cctaaattta aaattgctct agacaaacct ttaaaatcca ccccagagtg attcataaag
     4321 cgctcatctt ctgtagatat caacgcatta ataacatttg gagaaatgtc tttataggcg
     4381 acatttgttc tatttacttg ccaatacctt cctaattctg tttgtgcatc atcagcataa
     4441 atgatggaag ccaacatctc tggcggattg tccaacattt caatagatgg taaatcatct
     4501 tctgattgaa ttaacaacag aaatgcaatg atgattattg gcgtcatagc tcctagccat
     4561 aataaccaat aaagacgctt agtggttttc tcactaaaat tttctgtatc tttttttgca
     4621 gacattgcga aattaatttt gatgtaaatt taattaaaaa aacattgctt accagtagat
     4681 ggcaattttt ccgatagatt tacccgattc taataaatca tgagcttctg atagcgctgt
     4741 atgttggtaa tcaccaccat tttctgtttt tattttccct aatttataaa gctctaccac
     4801 cgcatttaaa caatgtttta acacttctgg cttgttatcg gcaatcttta acatattaac
     4861 cccaatgatg ctttttgact gcatcatttg aaaagctgga attagaacac ccattttgcg
     4921 taaaaaattg agttgcgata atatccccat tttaccttcg gaaagttgcg agcctccaaa
     4981 taaaaataat cgtgaaccaa aaccaagaac ttgtttgtct tttttgaagg ttgagccacc
     5041 tatggggtta aacgaggcat ctaatcgaaa tagttcgata tgtcgattta tgaattccac
     5101 ataatcttct ttcttatagt tcactgtaaa atcggccccc aattccttgc attttgcttc
     5161 tttttcagcc gaacttactt tagccacaac aaccgctcct gataattttg ctaattgtat
     5221 caacaacgtt ccaacacctc cagctgctgc atgaatcaat actttttctc ctatgcgtag
     5281 gggtgaaata taatttgcca tgtaataagc agtaactcct tgcgttgcca aggctaaagc
     5341 gttattggtt ggcatgtctc ctatttcaga aatagcatct atggtggtga tggcatgttg
     5401 tgcatagcca ccaaaacgtg taaaccctac tactcttttt cctattaaag attgctgttc
     5461 agacgctcct acttcaatta cttcgcctac aacttcatag cccaatacac aaggcattgg
     5521 cggtgcttcg cggtataaac cattgcgtgc catcacatca gcataattta atccaaaagc
     5581 ctccacttta atcagcactt cgtgtgattg caaagaaggt aattgtactg aattcatttg
     5641 aaatgcggtt gtggcatctc ctttttttac taattgatat gcttgtgtaa tcattctttt
     5701 gatttccctt caattattac aacaatttca cctttcggtt catgtttttc ataatgttgt
     5761 tttacttctt cggcggttcc acgttggtat tcttcgtata ttttagtcaa ttcacgaaca
     5821 acacacactt ggcgttcttt gcccataaat tcttcaattt gtcccaataa tttaactaaa
     5881 cggtggggcg attcatacaa aacaattgtt ctggattctt cggccaaaat ttgtaagcgt
     5941 gtttggcgtc cttttttgtg tggtagaaaa ccttcaaata caaatttatc acacggaaat
     6001 ccacttgcta ctagtgctgg aacgaatgct gttggtccgg gcaaacattc aatattaatt
     6061 tcgtttttta cacactcacg taccaataaa aatcctggat ctgaaattcc gggtgtacct
     6121 gcatcagata ccaacaccac taattcagaa gtttgaatac gttgcacaac attgtttaat
     6181 gcattgtgtt cattatgcgc atgaaaagga aaaacgtttt tagatatgtt taaatgcgct
     6241 aacaact
//
LOCUS       DAOQVQ010000096         6230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13888_length_6230_cov_4.049555, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000096 DAOQVQ010000000
VERSION     DAOQVQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13888_length_6230_cov_4.049555"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PKN22_04325"
     CDS             <1..1068
                     /locus_tag="PKN22_04325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS41962.1"
                     /translation="SWYPTELHPYLGNFVENQANLMSKEYQVTFLRLLPMGEKIPTPT
                     TAVNFQYVTVFYKHSRCSLLNYFAKKKAIQHALKNLTKVDLIHAHVLHPNGWQFRLAK
                     KMLKCPLVVTEHASYFATNFIWSKRMQWNIQKTMQKADVLIAVSSFLAQAIEKRLHVL
                     PHQIIGNPVDLNAFSNIPKLDDTIRFLHISTMSAVKNVKPILEAFKQVLENYPKAQLT
                     IVSDEDSTAIQQFAKQLGIQNSVVFAGPTEHSKMATYYQQASCFVLNSHFETFSIVVA
                     EAWACGIPVISTPVGIACKPNEGALIHTDGTTKSIAQAMLQFIENKANFDSSYIRSKA
                     TPFASETIFNQLNKVYNRLIG"
     gene            1115..3163
                     /locus_tag="PKN22_04330"
     CDS             1115..3163
                     /locus_tag="PKN22_04330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNS41963.1"
                     /translation="MKKTVLYVLASGGLLAACGTSKTNNQTQNSKALETVDFSYISKT
                     VKPTEDFFLFANENWIKNNQIPASESRWGSFNELEEANNAKLKGILDSLKNLHAPKGT
                     KEQLLGDYYASFTNMEVRNKLGIQPIQPLLNEIKALSSKKELAAMVAKLHRLGVNVLF
                     GYGVSQDMMNVDVNITYFGQGGIGLPNRDYYKDASKENIRTAYKKFIGDALVLAQLKD
                     ASSKDAVANQIFALEDKMATIMLKPEEQRIPELTYNKYAKQQLVADFKNFDLNAYLTG
                     IGSLAYDTLIVDNKKYATYVDEMIQTESLDAWKNYLLWHTVDRFMGELSQDFVDLNFD
                     FNGKTLSGKKEMKPIDERAIQAITYSGISEMLGHAFVDKYFSEAAKNRVNEMLDNLIV
                     AYRERIENLAWMSAETKKEAMNKLNSFGRKLGFPDKWEDFSSLNFAPDRYVENINEIS
                     NYMHLKDMKKLNEKVDRDKWEMPAHLVNAYYHPLLNEVAFPAGIMQAPFFDNNYEDAV
                     NYGRIGMVIGHEFTHGFDDMGSKFAADGSFKNWWTDEDRKAFEERTGIFGETFAKFCP
                     FEGQCVNPNLTMGENIADLGGLIMAYHAYTRTKEFKEGKVIGGYTPAQRFFIAYAQLW
                     KIKYTDAELKKRLATDPHSPGMYRINGPLKNCPEFFEAFGVKEGDAMRNPKNKVAIIW
                     "
     gene            complement(3213..3572)
                     /locus_tag="PKN22_04335"
     CDS             complement(3213..3572)
                     /locus_tag="PKN22_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNS41964.1"
                     /translation="MKYFWIGLGFLAVGLGVLGIALPLFPTTPFFLLAVFCFSKGSEK
                     FHKWFVATKMYKDYIESYRPGRPIKRSKKIEILTSVFIVLALSFYFVDSIALKIMLTS
                     IFVGHIIYFSFIIKTEK"
     gene            complement(3689..4978)
                     /locus_tag="PKN22_04340"
     CDS             complement(3689..4978)
                     /locus_tag="PKN22_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41965.1"
                     /translation="MMKSNLEQNFKDALDKYEMPYNPKAWEALNSKLDAMQPATKPAS
                     GSSLKWIGGISAAVVASSALIYAVMNNSTSTEVVNPTTTAQNTTQTVAPSNNEENTSK
                     QVETKTTSTSNAINSLDESNKEINSTVVSNNATQRNATSTTKTEVETNEKNTSYNPIV
                     KTPEQNNTTNDKMLAVEPSTPANLFNELNDLCLGESEQLKNKNAFPLTIISPSGNEQT
                     VAANQSVKFNPTESGTYTIASKNQKRTIVVKDAPKLDFEINEEVKYENGIPSIPLKTY
                     ADASNFVWSFEGQSTKQYGETATAHFYKQGEYTISLSAKNVDGCVATVKKQVSINENY
                     NLLAPTAFMPKSADSRKNKFIPYALTVRNTEFKMTIYEPRTGNVVFETTSIDGWDGID
                     INTRQMVDENKSFAWKVVLAHPEPGEPKEYSGIVVRM"
     gene            complement(4975..5535)
                     /locus_tag="PKN22_04345"
     CDS             complement(4975..5535)
                     /locus_tag="PKN22_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS41966.1"
                     /translation="MDINKVIEGCLRNDRRSQEQLFQLYYGKLIVVTLRYIKDRDTAE
                     EVLQNAFIKIFEKLDAYDFKGSFDGWSRRIVANTAIDFIRKSKKDPLLSGEDTDFVYA
                     TIDPVVEKEDFDHLSLKGEMALEAIQELSPAYRAVFNLFVLEEFSHKDIAEKLGISEG
                     TSKSNLAKAKANLKAILMERFTKIQQ"
     gene            5717..6136
                     /locus_tag="PKN22_04350"
     CDS             5717..6136
                     /locus_tag="PKN22_04350"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNS41967.1"
                     /translation="MATNRTFTMIKPDAVENGYIGKILDMITGAGFQIKAMKYTRLTE
                     EQAKKFYEVHAERPFYGELVEYMCSGPIVAAILEKDNAVADFRTLIGATDPAEAAEGT
                     IRKYYAESKGRNAVHGSDSDENAEIEGKFHFASSEIF"
BASE COUNT         2013 a         1005 c         1181 g         2031 t
ORIGIN      
        1 tcttggtatc ctaccgaatt acatccttat ttgggaaatt ttgtagaaaa tcaagctaat
       61 ttgatgagca aagaatacca agttactttt ttgcgcttgt tgccaatggg tgaaaaaata
      121 ccaacgccca caactgctgt taattttcag tatgttactg ttttttacaa gcattcacga
      181 tgttcattgc ttaactattt tgcaaagaaa aaagcaattc aacacgcgct caaaaacctt
      241 acaaaagttg atttaataca cgcgcatgtg ttgcacccca acggttggca atttagatta
      301 gcaaaaaaaa tgttgaaatg tccgcttgtt gtaacagaac atgcaagtta ttttgcaacc
      361 aattttattt ggagcaaacg catgcaatgg aacattcaaa aaacaatgca aaaagcagat
      421 gttttgatag ctgtttcttc atttttagcg caagcaattg aaaaacgatt gcatgtttta
      481 cctcaccaaa ttattggcaa tcccgttgat ttgaatgcat tttccaacat tcccaaatta
      541 gacgatacca ttcgtttctt gcacatttca acgatgagtg ctgttaaaaa tgtaaaacca
      601 attttagaag cattcaaaca ggtgttggaa aactatccaa aggcacagct aacaattgtt
      661 tcggatgaag attcgacagc tattcagcaa tttgctaaac aattagggat tcaaaattcg
      721 gtggtgtttg ctggtccaac agaacattca aaaatggcaa cttattacca acaagcatct
      781 tgttttgtgc ttaattctca ctttgaaact ttttccatcg ttgtggccga agcttgggca
      841 tgtggaatac ctgtaatttc tacacctgtt ggcattgctt gcaaaccaaa tgaaggtgca
      901 ttgatacata ccgatggtac tacaaaaagt attgctcagg caatgttgca attcattgaa
      961 aataaagcaa attttgattc ttcttatata cgctcaaaag ccactccatt tgccagcgaa
     1021 acaattttta accaattaaa taaggtgtac aatcgcttga ttggttaaaa aaacattaat
     1081 tttaggcaca caatcaaatt aagaaaatta aaggatgaaa aaaacggtac tttatgtgct
     1141 tgccagtggc ggattattgg ctgcttgtgg tacaagtaaa acaaacaatc aaactcaaaa
     1201 ttcaaaagct ttggaaacag tagattttag ttacatcagc aaaactgtaa aaccaacgga
     1261 agattttttc ttgtttgcaa acgaaaattg gattaaaaac aaccaaattc cggcgtcaga
     1321 atcacgatgg gggagtttta atgagttgga agaagcaaac aatgccaagt tgaaaggaat
     1381 tttagattca cttaaaaact tacacgctcc aaaaggaacg aaagaacaat tattaggcga
     1441 ttattatgct tcgtttacca acatggaagt gcgcaataag ttaggaattc aacctattca
     1501 accacttttg aatgaaatta aagctctttc atccaaaaaa gagttggctg caatggtggc
     1561 aaaattgcac cgtttgggag ttaatgtttt gtttggctat ggtgtttcgc aagacatgat
     1621 gaatgtggat gtgaatatta cttattttgg tcaaggtgga attggattgc ctaaccgcga
     1681 ttattacaaa gatgcatcaa aagaaaatat tcgcacggca tataaaaaat tcattggcga
     1741 tgctttggtt ttagctcaat taaaagatgc ttcgtctaaa gatgctgttg ccaatcaaat
     1801 ttttgctttg gaagataaaa tggcaactat catgttgaaa ccagaagaac aacgcattcc
     1861 agaattgact tataacaaat atgctaaaca acaattggtt gccgatttca aaaattttga
     1921 tttaaatgct tatttaactg gaattggttc tttggcttat gacacattga ttgttgacaa
     1981 taaaaaatac gcaacatacg tggatgaaat gattcaaaca gaatcgttgg atgcatggaa
     2041 aaactatttg ttgtggcaca ctgttgaccg ttttatgggt gaattgagtc aggattttgt
     2101 ggacttaaat tttgatttta acggaaaaac attgagcggt aaaaaagaaa tgaaaccaat
     2161 tgatgaacgt gccattcaag caataacata cagcggaata agtgaaatgt tgggtcatgc
     2221 ttttgtggac aaatatttct ctgaagcagc aaaaaatcgt gtcaatgaga tgttggataa
     2281 tttaattgtt gcctacagag aacgtattga aaatttggct tggatgtcgg ctgagacgaa
     2341 gaaagaagca atgaataaat tgaattcttt tggacgtaaa ttaggttttc ctgacaaatg
     2401 ggaggatttt agctcgttaa actttgcgcc cgatcgctat gtggaaaata tcaatgaaat
     2461 ttccaattac atgcacttaa aagacatgaa aaagttgaac gaaaaggtgg atcgcgataa
     2521 atgggaaatg ccagcacatt tagtaaacgc ttattaccat ccgttgttaa acgaagtggc
     2581 tttccctgct ggaattatgc aagcaccatt ttttgacaac aattacgaag atgcagtaaa
     2641 ttatggtcgc attggaatgg tgattggaca tgaatttaca catggttttg acgatatggg
     2701 ctctaaattt gctgccgatg gaagttttaa aaattggtgg acagatgaag accgcaaagc
     2761 atttgaagaa agaacaggaa tttttggtga aacatttgca aaattctgtc cgtttgaagg
     2821 tcaatgcgtg aatccaaact taaccatggg tgaaaatatt gccgacttag gaggtttgat
     2881 tatggcgtat catgcctata cgcgtacaaa agagtttaaa gaaggaaaag tgattggtgg
     2941 atatacgccg gctcaacgtt tctttattgc ttatgcgcaa ttgtggaaaa tcaaatatac
     3001 agatgctgaa ttgaaaaaac gtttggcaac cgacccacat tcaccaggta tgtatcgcat
     3061 caatggacca ttgaaaaatt gcccagaatt ctttgaagca tttggcgtga aagaaggtga
     3121 tgcaatgcgc aatcctaaaa ataaagtagc tattatttgg taaaacaaat agttgaatta
     3181 gtgaaaagcg atttctttgg aggtcgcttt ttttatttct ctgttttaat aatgaatgaa
     3241 aaataaatga tgtgtcccac aaaaattgaa gtcaacataa tttttaatgc gatgctatca
     3301 acaaaataaa agctgagggc aagtacaata aaaactgagg taagaatttc aattttttta
     3361 ctgcgtttta ttggtctacc agggcggtaa ctttcaatgt aatctttgta cattttagtt
     3421 gctacaaacc atttgtggaa cttttcagaa cctttagaga agcaaaatac ggccagtaaa
     3481 aaaaatgggg tagtggggaa caaaggtagt gcaattccaa gaacacccaa tcctacagcc
     3541 aaaaatccca atccaatcca gaagtatttc aaatcctaaa aatttgcacg aagatagtgt
     3601 ttgaatgtca taaaattgtc ataaaaaaaa cttcttgaag ctcaagaagt tttttaaatt
     3661 cataaatagc agcttagtcg tctctttatc acattcttac tacaattcca gaatattctt
     3721 ttggttcacc tggttctgga tgagcaagca ccactttcca agcaaatgat ttattttcat
     3781 ctaccatttg gcgggtgttg atatcaattc cgtcccaacc atcaatagaa gtagtttcaa
     3841 acactacatt tccagttctt ggttcgtaaa tcgtcatttt aaattctgta tttcttaccg
     3901 ttaaagcata agggatgaat ttatttttac ggctatcggc actttttggc ataaatgcag
     3961 ttggagctaa taagttgtaa ttttcattaa tgctcacttg ttttttaact gtagcaacac
     4021 agccatctac atttttagcc gacaaagaaa tggtgtattc tccttgtttg tagaaatgtg
     4081 cagttgctgt ttctccgtat tgtttggttg attgaccttc aaaactccat acaaaattag
     4141 aagcatctgc atacgtttta agaggaatag aaggaatacc attttcgtat tttacttctt
     4201 cgttgatttc aaaatccaat tttggagcat cttttacaac aatggtgcgt ttttgatttt
     4261 tagaggcaat ggtatatgtt ccactttcgg ttggattaaa tttcaccgat tgattggctg
     4321 caactgtttg ttcatttcca gatgggctga tgatggtcaa cgggaatgca tttttatttt
     4381 taagttgctc actttcaccc aaacacaaat catttaattc gttaaataaa tttgcaggag
     4441 ttgaaggttc aactgccaac attttatcat tggtagtgtt gttttgttct ggagttttaa
     4501 caattggatt gtatgatgtg tttttttcgt ttgtttctac ttccgttttt gtagttgaag
     4561 ttgcatttct ttgagttgca ttgttggaaa caacagttga atttatttct ttattagatt
     4621 catccagtga atttatcgca ttcgaagttg aagttgtttt tgtttcaact tgtttggatg
     4681 tattttcttc gttatttgaa ggggcaactg tttgtgttgt attttgcgcc gttgttgtag
     4741 gattaacaac ttctgttgag gtagaattgt tcatcacggc ataaatcaaa gcagacgatg
     4801 caactacggc agcagaaatt cctcctatcc attttaaaga agagccagat gctggtttgg
     4861 tagcaggttg catagcatcg agtttgctat tgagagcttc ccaagctttt ggattgtagg
     4921 gcatttcata cttatccaat gcatctttaa aattttgttc caagttactt ttcatcattg
     4981 ttgtattttg gtaaaccttt ccattaatat ggcttttaaa ttcgcttttg cctttgctaa
     5041 attagattta gaagttccct cagaaatgcc taatttttcg gcaatgtctt tatgtgaaaa
     5101 ttcttctaaa acaaagagat tgaaaactgc tcggtaagct ggagaaagtt cttgaattgc
     5161 ttcaagggcc atttcgcctt ttaacgagag gtgatcaaaa tcttcttttt ctacaactgg
     5221 atctatggta gcatacacaa aatcggtatc ttctcccgat agaagcgggt ctttttttga
     5281 ttttcggata aaatcaattg cggtgtttgc aacaattcta cgcgaccaac catcaaagga
     5341 gcccttaaag tcgtaggcat ctaatttttc aaagatctta ataaacgcat tttgtaaaac
     5401 ttcttctgca gtgtctctat cttttatata gcgtagagtt accacaatta gttttccata
     5461 ataaagttga aatagttgtt cttgtgaacg cctgtcgttt ctcaaacaac cttcaatgac
     5521 tttatttatg tccacacgtt tctccacttg cgttattcag ttttatgacg aattattttt
     5581 tctagggttg cctttttagt tattaaatgc taaaatttgg gtttaaaata catttttttt
     5641 gcaattgcag gataaactgt taaaaaaaaa taccttattt ttgcactcga ttttaaaagt
     5701 aaaagaataa aagaatatgg caacaaacag aacgtttaca atgattaaac cagatgcagt
     5761 agaaaatggt tatatcggaa aaattttgga tatgattact ggtgctggtt ttcaaatcaa
     5821 agcaatgaaa tatacacgtt tgacagaaga gcaagcaaaa aaattctatg aagtgcacgc
     5881 tgagcgtcca ttttatggtg aattggttga atacatgtgt tcaggaccaa ttgtagcagc
     5941 tattttggaa aaagataacg cagttgcaga tttccgtacg ttaattggtg ctactgatcc
     6001 agcagaagct gcagaaggta ctattcgtaa atattatgct gaatcaaaag gtagaaatgc
     6061 tgttcatggt tcagattcag atgaaaatgc agaaattgaa ggtaaattcc acttcgcaag
     6121 ctctgaaatt ttctaataat tgaatttttt tgaaatgaaa aaggttgtcg aaaggccacg
     6181 aatggtcgga agaaattccg gccatttttt atgttttaaa aaatagcttt
//
LOCUS       DAOQVQ010000097         6228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13896_length_6228_cov_4.731573, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000097 DAOQVQ010000000
VERSION     DAOQVQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13896_length_6228_cov_4.731573"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKN22_04355"
     CDS             <1..589
                     /locus_tag="PKN22_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNS41968.1"
                     /translation="LYTNEGEILLQNNADVSTKSAGGGTSTQYLVRRDGSVELPKLGS
                     VKIEGLSITQCEDTLEKLYSKEYNNPFVQAQITNQRVVVFPGNGSDAKVITLENNNTT
                     LMEAIAQAGGITERGKAKKVKLMRRIGDKRVVYVMNLSTIEGLKYADMIVQANDYIYV
                     EPNPQIAREVVKEVAPIVSILSSGIVIITVVRSLK"
     gene            592..2997
                     /locus_tag="PKN22_04360"
     CDS             592..2997
                     /locus_tag="PKN22_04360"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNS41969.1"
                     /translation="MAENKNSFFINKTFDPLLFRTVLRRSWWWIPLLMVVFFTFAKLY
                     LRYTKPIYESELLLQLENEDNAKNVLDIENVNTKKDVFYSDIELLKSELLFSNVLQSL
                     GMNVSVFYKGKFLTEEKYKSGSLHIVPFELKDSSLCDVPIYLNFENGKIKLKYWLGKE
                     NVISGKLDEHIKNDHFDIVIKSDNIENLKEEIAENEVYFIFNNKQSLIDRFIVNLGVL
                     PVDERARTVLINFRGDNPRICHDVTLAVANTFINYSNKAKKQSSESILSFIDMQLDSL
                     TSELKKSKDSLMNFQREVNLGNPETEDVTITSDVTKFQDELFAIEEETVTLQSIHKRL
                     MENPNRLDVYRILPELMGKSYESAIAGHIESLHTLLEEKEEILFRLTPESSEIKRMDK
                     KISEKTKQILRSVEAIEMRLKAKRKMLQGKLGELESEYASLPGKKMEYNRLKNIQDLN
                     EKYFQLLTEKKVQYAISDAGFSSQNRILKSPSISGTPVEPKPRLVYSVFILLGFIIGV
                     GILFFIYVSFNEINLLQDLQSILPQKATILGGVPQTKFALEHSQIVVTKSPKSMMSEA
                     LRKIRVNLNYIHPNYKTIAISSSISGEGKTFVALNLAGIISMTGKKTIILDLDMRKPK
                     VHLAFNLQNDNGMSNLIIDKITLDEAIHKDVEHNLDVITSGPIPPNPSELLLSKNFKK
                     LVEELKTKYDVVIIDNPPIGLVSDGIQILSEADIPIYVFKSQYSKRNFANRIKELFEL
                     KQIDSLNVILNGVAYTKQNMYGYGYGYGYGDYGGYVDDIKAGDLKLKKRTKFLTKFKK
                     KDA"
     gene            3000..3545
                     /gene="rfbC"
                     /locus_tag="PKN22_04365"
     CDS             3000..3545
                     /gene="rfbC"
                     /locus_tag="PKN22_04365"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNS41970.1"
                     /translation="MDIISTPIDDLLVFIPKKFEDSRGYFFESFNQQKFNQAVGKEVV
                     FVQDNESLSSKNVMRGLHFQTPPAAQGKLVRVVSGAVFDVAVDLRKNSPTFGKWHGEL
                     LSTENNKLMWIPEGFAHGFLSLDDNTKFLYKCTNYYSPGNEQTIIWNDPLINIDWKIS
                     EPIISEKDKKGATFTDFITPF"
     gene            3560..4630
                     /locus_tag="PKN22_04370"
     CDS             3560..4630
                     /locus_tag="PKN22_04370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNS41971.1"
                     /translation="MKFTIIQIILFTLGGFVASFITNSLLLNFSQSLGIRNKNDITVR
                     WSNQSKPSLGGISFFVVFIFFALAHSIVFKDFNAFLNRPYVGLITAGSLAFLMGLADD
                     AYNTKPLIKLTVQILCGAILVYSDSVIHLTNNFYIDSFITIFWVVLLMNSLNMLDNMD
                     GITGTVSLFIILTCLASDFIFQRFETNIFILQNFAMIGGLLAFLKFNINPSKIFMGDA
                     GSQFISLYVAFISIDSLWMTNHSTDLSFNWLGVVVVGLCYTAAFADTLTVVINRLRKG
                     QSPMVGGKDHTTHHLVYAGLKDKQVWYVFTFIALVASVLTLILLNWSVKGTSSWVVLF
                     VLFPLIVFYILFRNTQKHPEKK"
     gene            4682..>6228
                     /locus_tag="PKN22_04375"
     CDS             4682..>6228
                     /locus_tag="PKN22_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS41972.1"
                     /translation="MRLLVFTIFVNFLLLSFAQERVTITGVVKSNEDGESIVGAKIYF
                     SDIQKGAVTNQYGFYSITIPKGQHQLEISASDKDPLVKSINFQNDTTINVELFNPATS
                     INEVVVSAKKSENVNAVNMGQMELKIEEIKKLPAFMGEVDPIKAIQMLPGVSSVSEGS
                     QGFYVRGGGPDQNLVLLDEAPVYNASHLFGFFSVFNSDAIKSVNLIKGGMPANYGGRL
                     SSVLEVNMIEGNKKRFSVKGGLGVISSRLEVDGPIKKDKGSFMISGRRTYVDLLMKAF
                     IPKKSPFYGSSYYFYDLNFKANYQLSERDHIYLSGYYGKDQFKFGNKKDDFYVTMPWE
                     NGVGSLKWTHLFGRKLFMDVVTTYTHYKFAFNTEQDDIEFKSSTSINDVGAKIGFSYF
                     PNGSRHVMKFGAQYTYHIFNPSSISAKQDTTVFNTGISQNLYSHETGLYFLDEWNITE
                     DLKVNIGLRYSTFTHTGKFTRIIKGNIAQPDSLITYKNNEVVKFYQGLEPRFSIRYIL
                     PDKSSIKA"
BASE COUNT         2079 a          972 c         1157 g         2020 t
ORIGIN      
        1 attatacacc aacgaagggg aaatattatt gcaaaacaat gcagatgtta gcacaaaaag
       61 cgctggtggt ggaacatcta cacaatactt ggtaagaaga gatggctctg tggaattacc
      121 taaattgggt tctgtaaaaa ttgagggatt atccatcacc caatgcgagg acacactcga
      181 aaaattatac agcaaagaat acaacaatcc ttttgttcag gcacaaatca ccaatcagcg
      241 cgtggttgtt tttccaggta atggaagcga tgccaaagta ataacgcttg aaaacaataa
      301 tactacgttg atggaagcca ttgcgcaagc aggtggaatt acagaacgag ggaaagcaaa
      361 aaaagtaaaa ttgatgcgtc gaattggcga taaacgtgtc gtttatgtga tgaatttgtc
      421 aacaattgaa gggttgaaat atgccgatat gattgtacag gcaaacgatt acatttatgt
      481 ggaacccaat ccacaaattg cacgcgaagt tgttaaagaa gtggcgccta ttgtttccat
      541 tctttcgagt ggaatagtca tcataaccgt tgtccgatca ctgaaataat catggcagaa
      601 aataaaaata gtttttttat caataaaacc tttgatccgc tgctgtttcg aacagtattg
      661 cgtcgttctt ggtggtggat accattattg atggtggtat ttttcacctt tgcaaaactt
      721 tatttacgct ataccaaacc tatttatgaa tcggaattgt tgttgcaatt agaaaatgaa
      781 gacaatgcca aaaatgtatt agacattgaa aatgttaata caaaaaaaga tgttttttat
      841 tctgatattg agttattaaa atctgaatta ttgttttcca atgtattgca atctctggga
      901 atgaatgtga gcgtatttta caaaggaaaa tttttaaccg aagaaaaata caaatcagga
      961 agtttgcata ttgttccatt tgaattaaag gattcttcgt tgtgcgacgt tcctatttat
     1021 ttgaattttg aaaatgggaa aataaaatta aaatattggt taggaaagga aaatgtcatt
     1081 agtggaaaac ttgacgaaca tattaaaaac gaccattttg acattgtcat taaatcagac
     1141 aacattgaaa atttaaaaga agaaattgcc gaaaatgagg tttattttat tttcaacaac
     1201 aaacaaagcc taattgaccg ttttattgta aacttaggtg tgttacccgt tgacgagcgt
     1261 gcacgaacag ttcttatcaa ttttagagga gataatccaa gaatttgcca cgatgttact
     1321 ttagcggttg ccaatacatt tattaattat tccaataaag ccaaaaaaca aagttcggag
     1381 agtattttga gttttattga tatgcaattg gactcattga cctctgaatt aaagaaatca
     1441 aaggacagtt tgatgaattt ccaacgtgag gtgaacttag gaaatccaga aaccgaagat
     1501 gtaacaatta cttccgatgt aacaaagttt caggatgagt tgtttgccat tgaagaagaa
     1561 acagtgactt tgcaatccat tcacaagcga ttaatggaaa atccaaatcg attggatgtg
     1621 tatagaattt tacctgaatt aatgggaaaa tcgtatgagt cagcaattgc tggccacatc
     1681 gaatctttgc ataccctatt agaagaaaaa gaggaaattt tattccgctt aacacctgaa
     1741 agctcagaaa ttaagcgaat ggataaaaaa attagtgaaa aaacaaaaca gattcttcgt
     1801 agtgttgaag caattgaaat gcgattaaaa gcaaaacgaa aaatgcttca aggtaaatta
     1861 ggtgaattag agagtgaata cgctagtctt ccaggaaaaa agatggaata caatcgttta
     1921 aaaaacatcc aagacctcaa cgaaaagtat tttcaattac taaccgaaaa gaaagtgcag
     1981 tatgccattt ctgacgctgg tttttcatcg caaaatagaa ttttaaaaag cccaagcatt
     2041 agtggaactc cagttgaacc aaaacctaga ctggtgtatt ctgttttcat tttattaggg
     2101 tttattattg gggtaggtat tttattcttt atttatgtaa gttttaatga aattaactta
     2161 ttgcaagatt tacaatcaat tttaccccaa aaagcaacta tacttggggg agttcctcag
     2221 acaaagtttg ctttagaaca ttctcaaatt gtggttacca agtcgccaaa atcaatgatg
     2281 tctgaggcgt taagaaagat acgtgtgaac ttaaactaca ttcatccaaa ctataaaaca
     2341 attgccatat cttcgtccat ttctggagaa ggaaaaacat ttgttgcgct caatttggct
     2401 ggaattatct ccatgacagg taaaaaaacc atcattttgg atttagacat gcgtaaacct
     2461 aaagttcact tagcgtttaa tttacaaaat gacaatggaa tgagtaattt gattattgat
     2521 aaaatcacct tagatgaagc tattcataaa gacgttgaac acaatttaga cgtaattaca
     2581 tctggtccaa ttcctccaaa cccatctgag ttgttgttaa gtaaaaattt taaaaagcta
     2641 gtagaagagc tcaaaacaaa atacgacgtt gtaattatcg acaacccgcc tattgggttg
     2701 gtatccgatg gtattcagat tttatcagaa gcagatattc caatttatgt ctttaaatca
     2761 caatattcga aacgtaattt tgcgaaccgc atcaaagaat tgtttgaact aaaacaaatt
     2821 gattcattaa atgtcatact taacggtgtt gcttatacca aacaaaacat gtatggctat
     2881 ggttatggct acggatatgg cgattatggt ggttatgttg acgacattaa agcaggtgat
     2941 ttaaaattga agaaacgaac taagtttttg acaaaattta aaaagaaaga cgcataaaaa
     3001 tggatataat ttcaacacca attgacgatt tattggtatt tattccaaag aaatttgaag
     3061 atagtagagg ctattttttt gaaagtttta atcaacaaaa atttaatcag gcagttggta
     3121 aagaagtggt ttttgtacaa gacaatgaat cgctttcatc aaaaaatgta atgagaggtt
     3181 tgcactttca aactcctcct gcagcacaag gaaaactagt tcgtgtggta agtggagctg
     3241 tttttgatgt agccgttgat ttacgtaaaa attcaccaac ttttggcaaa tggcacggag
     3301 aattattatc tactgaaaat aataaactca tgtggatacc tgaagggttt gcacacggct
     3361 ttctttcgtt ggatgataat accaaatttc tttacaagtg caccaactac tattcacctg
     3421 gaaatgagca aactattatt tggaatgacc ctttaattaa tattgattgg aaaatttctg
     3481 aaccaataat ttcggaaaaa gacaaaaaag gtgctacatt tacagatttt attacaccgt
     3541 tttagtacga ctgttattta tgaaatttac tatcatccaa attatattat tcactttagg
     3601 aggctttgtt gcttccttta ttaccaattc tttgctattg aatttctcac aatcattggg
     3661 aattcgtaat aaaaatgaca ttaccgttcg ttggagcaat cagtcaaaac catcattggg
     3721 tggaattagt ttttttgtgg tgtttatttt ctttgcactt gcccactcta ttgtttttaa
     3781 ggactttaat gcttttttaa atcgacctta tgttggactt atcacagcag gttctttggc
     3841 atttttaatg gggttggcgg atgatgctta caataccaaa ccactaatta aattaactgt
     3901 ccaaattttg tgcggagcta tattagtgta ttccgattcg gtaattcatt taaccaacaa
     3961 tttttatatt gatagtttta tcaccatttt ttgggtggtt ttgttgatga attctttgaa
     4021 tatgctcgac aatatggatg gtattacagg cactgtttct ctgtttatca tccttacatg
     4081 tttggcttcc gactttattt ttcagcgttt tgaaaccaat atttttattt tgcaaaattt
     4141 tgccatgatt ggcggattac ttgcattctt aaaattcaac atcaacccat ccaaaatatt
     4201 tatgggcgat gcaggttctc aattcattag tctttacgtt gcttttatta gcatcgatag
     4261 tttgtggatg accaatcatt caacagattt atccttcaat tggttgggtg ttgtggtagt
     4321 tggtttgtgt tatactgccg cttttgcaga cacattaact gttgtaatca accgtttgcg
     4381 caagggacaa tcacctatgg tgggtggaaa agaccacaca acacaccatt tagtttatgc
     4441 aggattaaag gataagcaag tgtggtatgt gtttaccttc attgctctag tggcttctgt
     4501 attaacgtta atattgctca actggagtgt aaaaggtact tctagctggg tggtgttatt
     4561 tgttctattt cctttaattg tgttttatat tttgtttaga aatacacaaa agcatccaga
     4621 aaaaaaataa ttgtatttta tcaaatactt tattaatttt aactgccttt tttaagtatt
     4681 tatgcgtctg cttgtcttta ctatttttgt taacttttta ctattatcat ttgctcaaga
     4741 aagagtaacg attactggtg tagttaaaag caatgaagat ggcgagtcaa ttgtgggggc
     4801 taaaatttat ttttcagaca ttcaaaaagg ggctgtaacc aatcaatatg ggttttattc
     4861 tattaccatt cccaaagggc aacatcaatt agagatttcg gcaagcgata aagatccgct
     4921 cgttaaatcc attaattttc aaaacgatac cactatcaac gttgaattat ttaaccctgc
     4981 aacaagcatc aatgaagtag ttgtgagcgc caaaaaatca gaaaatgtga atgcagttaa
     5041 catggggcaa atggagctta aaattgagga aataaaaaaa ctaccagctt ttatgggcga
     5101 ggtggatccg attaaagcca ttcaaatgtt gcctggtgta tcgtctgttt ctgaaggtag
     5161 tcaagggttt tatgtgcgtg gtggcggtcc cgaccaaaat ttggtattgt tagatgaagc
     5221 gcccgtgtac aatgcctctc acctttttgg ctttttctcg gtatttaatt ccgatgccat
     5281 taaaagcgtt aatttgataa aaggtggaat gcctgccaat tatggtggtc gactttcatc
     5341 tgttttagaa gtaaacatga ttgaagggaa taaaaaacgt ttttcagtaa aaggtggatt
     5401 aggtgttatt tcatctcgct tggaggtgga tggacctata aaaaaagaca aaggctcgtt
     5461 tatgatttct ggtagaagaa cctatgttga tttgctcatg aaagcattta tccccaaaaa
     5521 atcaccgttt tatgggtcga gttactattt ctacgactta aatttcaaag ccaattatca
     5581 actttctgaa cgtgaccata tctatttgag tggttattat ggtaaagatc aattcaaatt
     5641 tggcaataaa aaagatgatt tttatgtaac gatgccttgg gaaaatggcg ttggttcatt
     5701 gaaatggaca catttatttg gtcgaaaatt gtttatggat gttgtaacga cctatactca
     5761 ttataaattt gcttttaata ccgaacaaga cgacattgag tttaaatcca gcacaagcat
     5821 caacgatgta ggggcaaaaa ttggtttttc atacttcccc aatggtagtc gccacgtgat
     5881 gaaatttgga gctcaatata cttatcatat tttcaatcct tcgagtattt ctgcaaaaca
     5941 agatacaaca gtatttaaca ctggaataag ccagaattta tacagccatg aaactggact
     6001 ttacttctta gatgaatgga acattaccga agatttgaaa gtgaacatcg gattgcgtta
     6061 tagcaccttt acgcatacgg ggaaatttac acgcattatc aagggaaaca ttgctcaacc
     6121 cgattcgtta attacctata aaaacaatga agtagtaaaa ttttatcaag ggttagagcc
     6181 tagattttca attcgttaca ttttacctga taaaagttca ataaaagc
//
LOCUS       DAOQVQ010000098         6197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_13992_length_6197_cov_3.910615, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000098 DAOQVQ010000000
VERSION     DAOQVQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13992_length_6197_cov_3.910615"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKN22_04380"
     CDS             complement(<1..427)
                     /locus_tag="PKN22_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41973.1"
                     /translation="MKFILSLLLAVITTSSFSQEIADLFRKSDTKFTWLGIDYSHVKL
                     IGDFSQIWGIDTQDGLSIKTEYFPAWNNFVYKEPDKYNVIATFRKEHIAINLDDIDKI
                     NRSTPLEEMEAITTPNYTQENIQQFIKDHQFTEKEGIGLL"
     gene            526..1272
                     /gene="fabG"
                     /locus_tag="PKN22_04385"
     CDS             526..1272
                     /gene="fabG"
                     /locus_tag="PKN22_04385"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNS41974.1"
                     /translation="MKLLQDKVVLITGASRGIGKSIAEECVKHGAKVAFTYLSSEEKA
                     KALEAELTKNGGEVKGFKSDAAKFDDAQKLVDDVVAAFGTIDVLVNNAGITRDNLLMR
                     MTEEQWDEVINTNLKSAFNLTKAVQRPMLKAKSGSIINVSSVVGVSGNAGQANYAASK
                     AGLIGFTKSIAQELGSRNIRANAIAPGFIETEMTDVLDPKVVEQWRASIPLKRGGKPE
                     DVANLVVFLGSDMSAYVTGQTINVCGGMLM"
     gene            complement(1362..2315)
                     /locus_tag="PKN22_04390"
     CDS             complement(1362..2315)
                     /locus_tag="PKN22_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNS41975.1"
                     /translation="MKPPYEITSEIVQLISSISEKIGEVNAKYLIKTNPTLRKQNQIK
                     TIHSSLSIEGNTLSLEQITAIIENKRVVGPEKDILEVLNALEVYKNIHRLKPDSEKDF
                     LKAHKLLLQQLIKNPGEYRKQGVGIVKGTNIEHIAPPYENVPFLMRDLFTYLKDKTEL
                     TLIKSCVFHYEMEFIHPFIDGNGRMGRLWQTLILMQDYPIFEFLPFETLISKNQTEYY
                     KALAISDKEGKSTKFIAYMLMIINRSLSELLANSAKRLNNEDRIQIFLDNNTGEFSRK
                     KYMDYFKDISTATASRDLKNAVEKGILIKTGDKKTTVYKKK"
     gene            complement(2434..4536)
                     /locus_tag="PKN22_04395"
     CDS             complement(2434..4536)
                     /locus_tag="PKN22_04395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNS41976.1"
                     /translation="MKNNKILILSLTLIAMACKSKVEPPKSLSDNPLLVESTLPYFAP
                     DFSKIKDEHFKPALEEGMRLQNEAIQAIVESSDAPTFENTVVALEKSGATLSSVNAVF
                     NALAAANTNETLQDLEEEMAPKLSEHFDAIYLNDKLFNRIETLYNKRDQLKLDAESAK
                     LLEHYYTNFIFSGANLSAENKEKLKKLNSREATLTTEFSKKVLAAMNDGGVTFDSAEE
                     LKGLDTAYLKSIQTKDGKYFIPLINTTQQPDLQYLQNSASRKKLFDASWKRCDGTKND
                     TKKIIVELVDLRCQKAILLGFANYASWAMQNTMAKTPENVREFFNQLIPAAIGKAQKE
                     AEIIQESMAKSGDKATLTAADWSIYAEKVRKEKYNVDESEIKPYFEVMNVLEDGVFYA
                     ATKLYGITFKKRTDIPVYHPDVLVYELFNKDGSQLGLFYADLYARPSKNGGAWMDNLV
                     TQSKMHNRKPVIYNVMNIPKPAEGEACLVSFDNVITLFHEFGHALHGFFADQHYPSLS
                     GTAVPSDFVEFPSQFNENWATHPVVIKNYAKHYQTGKQIPAELLEKMQAAATFNQGFS
                     MTETIAAASNDIEWHMVGADKKITDVNAFEKNALARYGFDAIEAVKPRYRSTYFSHVF
                     SGGYAASYYAYLWTEMLDHDAYQWFEENGGLTLANGQRFRDMILSRGNTQELAQMYRN
                     WRGKEPVIEPMLKARGLK"
     gene            4679..5542
                     /locus_tag="PKN22_04400"
     CDS             4679..5542
                     /locus_tag="PKN22_04400"
                     /inference="COORDINATES: protein motif:HMM:NF026866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 23 domain-containing
                     protein"
                     /protein_id="HNS41977.1"
                     /translation="MSFGQGVRWSKPNAGVQAGLVLEAGTHELAIGFHLKSFIGFDYA
                     QLNVGNTFMWKFYSLGKREMFYENRFYAGGALMVGKNQSVVDFELDGLNHQTKKNFAV
                     AYNFLIYSDNAGTSQLSGAFGMHIKNVSIKFENDVFSGQAKDRFRTGILDISYRTPTF
                     KINTGFYIWTGETAGSFWDKSVKTKKMPSGYRSLEDLPYGKTTHGIVYGGIKYLMHFG
                     NVVYARIGVDSEQFRHGLQNRLIHDMAFLPQTFPRNTPHYPRLDENGCPVFEKELVRK
                     PKFYLQFGINE"
     gene            complement(5560..6048)
                     /locus_tag="PKN22_04405"
     CDS             complement(5560..6048)
                     /locus_tag="PKN22_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNS41978.1"
                     /translation="MKTYKWWIIVINLLLLLVYFAYSVASKEDLTKNGRLILLELAPV
                     DPRSLLQGDYMDLRYKISEEVNYQNIPKNGYCVVTLNDKNIAKRVRFQPHKQALNEGE
                     YVIKYTTKNSWQINIGAESYFFQEGHAKKYEAAKYGGLRIDENGNSLLIGLYNEDLQK
                     IE"
BASE COUNT         1856 a         1072 c         1157 g         2112 t
ORIGIN      
        1 aaagcaatcc aatcccttct ttttctgtaa attgatgatc ttttataaat tgttggatgt
       61 tttcttgcgt gtaattaggg gttgttatcg cttccatttc ttctaatggt gttgaacgat
      121 tgattttatc gatatcatcc aaattaatag cgatgtgttc tttacgaaat gttgcaatca
      181 cattgtattt atcaggttct ttataaacaa aattattcca agctggaaaa tattcggttt
      241 taatagataa cccatcttgg gtatctattc cccaaatttg actaaagtca ccaattaatt
      301 taacatgtga ataatcaatt cctaaccagg tgaatttggt gtctgatttt cgaaataagt
      361 cggcaatttc ttggctaaaa gatgatgttg taataactgc tagtaaaagg gataaaataa
      421 atttcatact tttttttatt ttcaaagcta caaaaaggta atgaaaaatt caaatgattt
      481 gtcaaattaa tctttttagc tatttttggt ttaaaataaa gacaaatgaa attattacaa
      541 gacaaagtag ttcttatcac aggagcatca agaggaattg gaaaatcaat tgctgaagag
      601 tgtgtaaaac acggcgcaaa agtagctttt acttatttgt catccgaaga aaaagcgaaa
      661 gctttagagg cggaattgac caaaaatgga ggagaagtga agggttttaa atccgatgca
      721 gctaaatttg acgatgcaca aaaattagtg gacgatgtgg tggctgcttt tggaacaata
      781 gatgttttgg ttaacaatgc aggaatcaca agagacaatt tgttgatgcg tatgaccgaa
      841 gagcaatggg atgaagttat taataccaac ttaaaatcgg cgtttaacct tacaaaagct
      901 gttcaacgcc ctatgttgaa agcaaaatcg ggttcaatta tcaatgtttc atcggttgtt
      961 ggtgtaagcg gaaatgctgg tcaggctaac tatgcggcat caaaagctgg attgattggt
     1021 tttacaaaat caattgcgca agaattgggg tcgcgtaaca ttcgtgccaa tgccattgca
     1081 ccaggtttca ttgaaacaga aatgacagat gtgttggatc caaaagtggt ggaacaatgg
     1141 cgtgcttcta ttccgttgaa aagaggtgga aaaccagaag atgttgccaa cttggtagtg
     1201 tttttaggtt ctgatatgtc agcctatgtt acaggacaaa ccatcaatgt ttgcggtggt
     1261 atgttgatgt aatcaaaaat gataaaacga aaggcgttgt ttgaaaaagc aacgcttttt
     1321 ttgtggggat aattatgctt taatccattt aactcactcc atcatttctt tttgtaaaca
     1381 gttgtttttt tgtcgccagt tttaattaga attccttttt ctacggcatt ttttaaatcg
     1441 cgactggctg ttgcagtgga aatatcctta aagtaatcca tgtatttttt acgactaaat
     1501 tcaccggtgt tgttgtcaag aaaaatttga attctatctt cgttatttaa tcgcttagca
     1561 gaattagcaa gtagttcaga taatgaacga ttaataatca tcagcatgta tgcaataaat
     1621 tttgtggatt tcccttcttt gtccgaaatt gctaaagcct tgtaatattc agtttgattt
     1681 tttgaaatta atgtttcaaa tggtagaaac tcaaaaattg gatagtcttg cattaaaatt
     1741 aatgtctgcc ataaccttcc cattcttcca tttccgtcta taaaaggatg tatgaactcc
     1801 atttcgtaat gaaaaacaca acttttgatt aacgttaact ctgttttatc ttttaaatag
     1861 gtaaataaat cgcgcatcaa aaacggaaca ttttcataag gtggagcaat gtgttcaata
     1921 ttggtgcctt taactattcc aactccttgt tttctatatt ctcccggatt tttaatgagt
     1981 tgttgcaaga gtaatttatg cgcttttaaa aaatcttttt cactatcagg ttttaaacgg
     2041 tgtatattct tatacacttc taaggcattc aaaacttcaa gaatgtcttt ttctggaccc
     2101 accactcgtt tgttttcaat aattgcagtt atttgctcaa gacttaatgt gtttccttca
     2161 atactaagtg aagaatgaat ggtttttatc tgattttgct ttcttaatgt tggattggtt
     2221 ttaattaaat attttgcatt tacttcacct attttctcag aaatagagga gatgagttgc
     2281 acaatctctg atgttatttc gtaggggggc ttcataaatt gatactatca tttgatacta
     2341 tcacaaattt aatttaaaaa atgaattaga gtgtgatatt ctataaaaaa aagcgctgct
     2401 ttcatcaaac agcgcttact aaattcaaat attttacttc aatcctcttg ctttgagcat
     2461 gggctcaatt acaggttctt ttcctcgcca attgcggtac atttgtgcga gttcttgcgt
     2521 attgcctcgc gatagtatca tgtctctaaa acgctgacca tttgctaaag ttaaaccacc
     2581 attttcttca aaccattggt aggcatcgtg atccaacatt tctgtccaca agtaagcgta
     2641 gtaacttgca gcatatccgc cactaaaaac gtgtgagaaa taagtggaac ggtagcgcgg
     2701 ttttaccgcc tcaattgcat caaatccata acgtgccaag gcgtttttct caaatgcgtt
     2761 gacatcagtg atttttttat ccgctcccac catgtgccat tcaatatcgt tgcttgctgc
     2821 cgcaatggtt tctgtcatac taaaaccttg attaaaagta gctgccgctt gcattttttc
     2881 taacaactca gctggaattt gttttccagt ttggtaatgc ttggcgtagt ttttaataac
     2941 aacaggatgt gttgcccaat tttcgttgaa ttgcgatgga aattccacaa aatcacttgg
     3001 aacggctgtg cccgacaaac taggatagtg ctggtcggca aagaaaccgt gcaaggcgtg
     3061 accaaactcg tgaaacaatg taataacgtt gtcaaaactt actaaacatg cttcaccctc
     3121 tgctggtttt gggatgttca ttacgttgta aattaccggt ttgcggttgt gcatttttga
     3181 ttgggttact aaattatcca tccacgcacc accattttta gaagggcgag catataaatc
     3241 ggcataaaat aaacctaact gcgaaccgtc tttgttaaac aattcataca ccaacacatc
     3301 gggatgatac actggaatgt cagtgcgttt tttaaatgta ataccgtata atttggtagc
     3361 agcataaaaa acaccgtcct ccaacacgtt catcacttca aaataaggtt ttatttcgct
     3421 ttcgtccacg ttgtattttt cttttcgcac tttttcggca taaatgctcc aatcagctgc
     3481 ggttaacgtt gctttatcgc cactttttgc cattgattct tgaataattt cggcttcttt
     3541 ttgtgccttg ccaattgctg ctggaattaa ttgattaaaa aactcacgca cattttctgg
     3601 agttttcgcc attgtatttt gcattgccca cgaagcataa ttagcaaaac caagcaatat
     3661 ggctttttga cagcgtaaat ccaccaattc tacaatgatt tttttagtgt cgttttttgt
     3721 tccatcgcaa cgtttccacg aagcatcaaa taactttttg cgacttgcac tgttttggag
     3781 gtactgcaaa tcgggttgct gtgtagtgtt gattagcgga ataaaatatt tgccatcttt
     3841 ggtttgaatg gattttaaat aagctgtatc caaccctttc aattcttcgg cagaatcaaa
     3901 ggttacgccg ccatcgttca tagctgccaa tacttttttg ctaaattcgg ttgttaaagt
     3961 ggcctcgcgt gaattgagtt ttttcaattt ttctttattc tctgccgaaa gattagctcc
     4021 tgagaaaata aaattagtgt aataatgctc caataattta gccgattctg catccaattt
     4081 caattgatcg cgtttgttgt aaagcgtttc aatgcgatta aataatttat cgttgaggta
     4141 aatagcatca aaatgttcgg ataatttagg tgccatttct tcttctaaat cttgcaatgt
     4201 ttcattggtg ttggctgctg caagtgcatt gaacacggcg ttaacactcg aaagtgtagc
     4261 tccacttttt tccaacgcaa caacggtgtt ttcaaaagtt ggcgcatcag aactttctac
     4321 gattgcttgg atggcttcgt tttgtaaacg cataccttct tctaacgcag gtttaaaatg
     4381 ttcgtctttt atttttgaga aatcgggagc aaaataggga agtgtgctct caactaataa
     4441 agggttgtcg gataaggatt taggtggttc aactttactt ttacaagcca ttgcgattaa
     4501 cgttaatgaa agaattaata ttttgttgtt tttcattggg ttgattttta gaaggataaa
     4561 gataattttt aattttaaaa tagtacacca aacaacggtt aactttggaa cgttttttga
     4621 aaataaagac aacgtgaatc aaaaattgat acttattctt gcgtgttgcc ttagtttttt
     4681 gagctttggt cagggggttc ggtggagcaa acccaatgct ggtgtgcaag ctggcttggt
     4741 gttggaggca ggaacgcatg aattggcaat aggttttcat ctcaaaagtt ttattggttt
     4801 tgactacgcg cagttgaatg taggcaacac gtttatgtgg aagttttaca gcttgggtaa
     4861 acgtgaaatg ttttacgaaa acagattcta tgcaggtggt gcactcatgg tgggcaaaaa
     4921 tcaatcggtg gtggattttg aattggatgg attgaaccat caaacaaaaa agaattttgc
     4981 tgttgcctat aattttttga tttactcgga taatgcgggc acttcgcaat tgtcgggtgc
     5041 ttttggaatg catattaaaa atgtttccat caaatttgaa aacgatgtgt ttagtggaca
     5101 agccaaagac cgatttagaa ctggaatttt agacattagt tatcgcacac ctacttttaa
     5161 aatcaataca gggttttaca tttggacggg cgaaacggca ggatcttttt gggataaatc
     5221 ggtgaaaact aagaaaatgc caagtggcta tcgttctttg gaagatttgc cttatggaaa
     5281 aacaacgcat ggtatagttt atggcggaat caaatatttg atgcattttg gtaatgttgt
     5341 atacgctcgc attggtgtgg atagcgaaca atttcgtcat ggacttcaaa accgcttgat
     5401 acacgatatg gcgtttttac ctcaaacatt tcctagaaat acgccacatt acccccgatt
     5461 ggatgaaaat ggttgtccag tttttgaaaa agaattggtc agaaaaccca aattctacct
     5521 tcaatttgga atcaatgaat agcaatttca aaaaatagct tattcaattt tttgcaaatc
     5581 ttcgttgtac agtccaatca acaaactatt gccattttca tcgatgcgca aaccaccata
     5641 tttagcagct tcgtattttt tggcgtgccc ctcttgaaaa aagtaggatt ctgcaccaat
     5701 atttatttgc caactatttt ttgtggtgta tttgattaca tattctcctt cattcaaggc
     5761 ttgtttgtgt ggttgaaatc gaactctttt tgcaatattt ttatcgttaa gagtaacaac
     5821 acaataccca tttttaggaa tgttttgata attcacttct tccgaaattt tgtagcgcaa
     5881 atccatataa tcgccttgta gtaacgagcg cgggtcaact ggtgccaatt ccaacaaaat
     5941 caatcggccg tttttggtta gatcttcttt gcttgctacc gaataagcaa aataaaccag
     6001 caacaacaat aggtttatca caattatcca ccatttatat gttttcatct gcatttaatt
     6061 ttttagttac caaccaatac agcgcaaaaa acaatgcgcc agaaacaagc attaaaattg
     6121 atttaaaaag caaattgatg ctcaaatcgt agtaaaattg aactataaaa taaattaagg
     6181 taattatccc caacaca
//
LOCUS       DAOQVQ010000099         6102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14290_length_6102_cov_3.179097, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000099 DAOQVQ010000000
VERSION     DAOQVQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14290_length_6102_cov_3.179097"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1083
                     /locus_tag="PKN22_04410"
     CDS             22..1083
                     /locus_tag="PKN22_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS41979.1"
                     /translation="MKHLFFSGVLLLLSTPTFSQITITHTSLPQPGDTFAMHYDANPT
                     INYGTASSSSQHFDFTQLQTDEDKYAAYGITANLPFASEFPQSNLYTYGPSVIYGGPG
                     ASVPTGNGWMLFRTDVNGMDVIGYKTDNGANDVSTVEVPPRMLMRTPCTLGTNETQDA
                     VWTYVYDAVATDYDTIVKSFSHTVLNCDAWGTISTPVASNVDVIRVNKTTLMIDSAYV
                     VMNGITYWKGEIQRASSNNFDFYATTKRHPIATVFCDQNGTAYGAEYLFYEDLYNSTE
                     NLTSTSFSIYPNPVKNQLKISTNASNLSYEIVDINGSLIQAGAVNNNIIDTKELNAGL
                     YLIRLSSPSSIQTLRFVKE"
     gene            1345..2289
                     /gene="nadA"
                     /locus_tag="PKN22_04415"
     CDS             1345..2289
                     /gene="nadA"
                     /locus_tag="PKN22_04415"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNS41980.1"
                     /translation="MKREELKAEILRLKKEKNTVILAHYYQEDDIQEIADFVGDSYGL
                     SVEASKTNAEMILFCGVHFMAETAKILNPTKKVLLPDLNAGCSLADGCKYDEFKAFKD
                     EHPDHEVVMYINCSAEVKSISDYICTSSNAVKIVNSIPADKKVIFAPDRNLGRYVKKA
                     TGRDLVLWNGKCIVHEAFSFDKIMKLILAHPNADLIAHPESTADVLELAQFVGSTAAM
                     INYVKESPKDEFIIATEAGILHKMHEVVPHKTLIPAPIDEDNTCACSECAFMKMNTLQ
                     KVYDCLVNEYPVIDLTTEVMDAARLPIERMIQITESAK"
     gene            2279..3808
                     /gene="nadB"
                     /locus_tag="PKN22_04420"
     CDS             2279..3808
                     /gene="nadB"
                     /locus_tag="PKN22_04420"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNS41981.1"
                     /translation="MQNKSDIVVVGSGIAGLSFAIKIAEKMPNCTINVLTKSQVALSN
                     SNFAQGGIAVVTNLVKDSYEAHIQDTLASGGGLSKPSIVKLVIETAYERLKELVAYGV
                     EFNKTPEGDFDLALEGGHSTHRVVHSYDHTGENVVKGLIKKASTFKNIQFFENRFCIE
                     LIKNEHGAVKGLTALNLNTNELETYVSNLVVLASGGSGQVYKYTTNPDVATGDGVAMG
                     IKCGAMVSNLNFIQFHPTALYEENKPRLDLLTEAIRGFGAHIVNKNGKRFVFDYDNRG
                     ELATRDIVSKAIFSELRKSDEKCVYLDCKHLDKDKFNEKFPQIAANLQSKNLDIAIDL
                     IPVVPATHYQCGGLKVDVDGQTTVTGLFAIGECAETGLHGANRLASNSLLEGLVFAHQ
                     AANFIAENYEQFVSDSSIESPTYTLDKSNDAVFETAIDQLRNTMSKYATIASSLDDIE
                     LCMQQLDAINNSIESIKNNQIISEQRLIFNNLMVNARAIAKFKRLHILHAQKTETLEK
                     H"
     gene            3825..5225
                     /locus_tag="PKN22_04425"
     CDS             3825..5225
                     /locus_tag="PKN22_04425"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNS41982.1"
                     /translation="MENKKQNRLRTLTEPFKIKVVEPLKVTAEEYREEALEKAGYNPF
                     LLSSKDVFIDLLTDSGTGAMSDNQWGAIMVGDESYAGARSWEKLENVVKDLTGMPYVL
                     PTHQGRAAERILYSTIGGPNKVFISNTHFDTTRANIEFVGSTAIDIVCPEAFDNQSDY
                     PFKGNIDLQKLEALIAQYGKENIAAVIMTVTNNSSGGQPVSMENFKAVRKICDANGIF
                     LVMDGCRIAENSYFVHHKEAACSNKTYREIAQEMFALSDAFVMSAKKDGMVNMGGLLT
                     LRDEALSVKCKNLLIISEGFTTYGGLSGRDMEALAVGLVEVFDEDYLHYRIKSTTYLG
                     EGLRKKGIPVVWPIGGHAVYIDAKELYKNIPLKEYPGQALVCDLYLKGGVRTVEVGSV
                     MFGKYDENGELIPAEKELVRLAIPRRVYTQSHIDYVLDVFDDILEMRDRKNGYEITYE
                     PPFLRHFTAHFKPINK"
     gene            5242..>6102
                     /locus_tag="PKN22_04430"
     CDS             5242..>6102
                     /locus_tag="PKN22_04430"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNS41983.1"
                     /translation="MAKLEKRSWAEPYRMKMVELLKMTTKAQREKALKEAGYNTFLLH
                     AEDVYIDLLTDSGTNAMSDRQWSAFMTGDEAYAGSKSFYLMEKAIQQFYGYKYVIPTH
                     QGRGAENLLSQILIKKGDFVPGNMYFTTTRLHQELNGATFVDIIVDEAHDPQDESPFK
                     GNVDISKLDALVKKEGAKKIPYVCIATNVNMAGGQPISIKNLKELREYTNKHGIKIIH
                     DMTRVAENAYFIQQREEGYKNVSIKDIVKEICSLTDGATMSAKKDALVNIGGFMATND
                     WDVYEEARNLV"
BASE COUNT         2063 a         1047 c         1214 g         1778 t
ORIGIN      
        1 ttttttaaat ttttagaact tatgaaacat ctattttttt ctggtgtact attattgtta
       61 tctacaccaa ctttttctca aataactatc actcacactt ctttgccaca acctggcgat
      121 acatttgcaa tgcattacga tgccaatcca acaattaatt atggtacggc ttcatcatca
      181 tcccaacatt ttgattttac gcaattgcaa accgatgaag ataaatatgc tgcctatggt
      241 ataaccgcca atttgccttt tgcaagtgag tttccacagt ctaatttata tacgtatgga
      301 ccttctgtaa tttatggtgg acccggagct tctgttccaa caggaaatgg ttggatgttg
      361 ttcagaacgg atgtcaacgg aatggacgta attggttata aaaccgataa tggggctaat
      421 gatgtttcaa ccgttgaagt tccaccacgt atgttgatgc gaactccttg cactttagga
      481 acgaatgaaa cacaagatgc tgtttggacc tatgtgtacg atgctgttgc aacagattat
      541 gataccattg ttaaaagttt tagccatacg gtattaaatt gcgatgcttg gggaacaatc
      601 agcacacctg ttgcaagtaa tgtggatgtt attcgtgtta acaaaacaac actaatgatt
      661 gattctgctt atgttgtaat gaatggtatt acctactgga aaggagaaat tcaacgtgca
      721 tcatccaata attttgattt ttatgcaacc actaaacgcc acccaattgc aaccgttttt
      781 tgcgatcaaa atggaactgc ttatggtgcc gaatatttgt tttatgaaga tttatacaat
      841 tcaacagaaa atttaacttc aacatcgttt tcaatttatc ccaatccagt taaaaaccaa
      901 ttgaaaatat ctacaaacgc ctccaattta tcctacgaaa ttgtagatat taatggttct
      961 ttaattcaag ctggcgctgt aaacaataac attattgata caaaagaatt gaatgcagga
     1021 ttgtacctca tccgattgtc atctccttca tctatccaaa ccttgcgatt tgttaaggaa
     1081 tagataaaac aattgattct acaaattatt ttaacaaaat gtgataaata aataaaaaag
     1141 tattgcattt gttaaaatcc aattatataa aagactttta agtccgattt attgattttt
     1201 attttatatt tgtcgaaatt aatgcgtgaa atcataaaat acttcataaa ctgatttccc
     1261 ctttgattga atgaccagat tattactttg aaaaaaacta tactttttaa ttgtacgcat
     1321 tgagctaagt aataagaatt taaaatgaaa agagaagaat taaaggcgga aatcttacgg
     1381 ttgaagaagg aaaaaaacac ggtaattttg gcccattatt accaagaaga tgacattcaa
     1441 gagatagccg actttgttgg agacagttat ggattatcag tagaagcatc taaaacaaac
     1501 gctgagatga ttttgttttg tggggtgcat tttatggcag aaaccgcaaa aattttaaat
     1561 ccaacaaaaa aagtattgtt acctgatctt aatgcaggtt gctcacttgc agatggttgt
     1621 aaatacgatg aatttaaagc ttttaaagac gaacatcccg atcatgaagt ggtgatgtat
     1681 attaactgtt ctgctgaagt aaaatctatt tcagattaca tctgtacatc ttccaatgcc
     1741 gtaaaaattg tcaattctat tccagccgat aaaaaagtga tttttgctcc cgaccgcaat
     1801 ttgggtcgct atgtaaaaaa agctactggt cgtgatttgg tgctatggaa cggaaaatgc
     1861 attgtgcatg aagctttttc ttttgataaa atcatgaaac ttattcttgc acaccctaat
     1921 gccgatttaa ttgcgcatcc agaatcaacg gcagatgtat tagaactagc acaatttgtt
     1981 ggttcaactg ccgcaatgat taattatgtg aaagaatcac caaaagatga gttcatcatt
     2041 gctactgaag caggaatttt acataaaatg cacgaagtag tgcctcacaa aacattaatt
     2101 cccgcaccaa ttgatgaaga taatacatgt gcgtgtagtg aatgcgcatt tatgaagatg
     2161 aacacgcttc aaaaagtgta cgattgtttg gtaaacgaat atccagttat tgatttaaca
     2221 acagaagtaa tggatgctgc acgtttgcct attgaacgaa tgatacaaat tacagaaagt
     2281 gcaaaataag agcgatatag tagttgtagg ctctggaatt gccggactat catttgcaat
     2341 aaaaattgca gaaaaaatgc ctaattgtac aatcaacgtg ttgacgaaat cacaagtggc
     2401 attgtcaaat tctaattttg ctcaaggtgg tattgccgtt gtaaccaatt tggtaaaaga
     2461 ttcctacgaa gcccatattc aagatactct tgctagtggt ggcggtttat ctaaaccttc
     2521 catcgtaaaa ttggtaattg aaacagctta tgagcgtttg aaagaattgg tggcttatgg
     2581 cgttgaattt aacaaaaccc ctgaaggtga ttttgactta gcgctggaag gcggacacag
     2641 cacccatcgt gtggtgcatt cctatgatca cacgggtgaa aatgttgtaa aaggcctaat
     2701 caaaaaagca tcaactttta aaaacattca gttttttgaa aaccgatttt gcattgaact
     2761 cattaaaaat gaacacggag ctgtaaaagg attaacggca ttgaacctca acaccaacga
     2821 attggaaact tatgtcagta atttggtggt gctggcatct ggtggttcgg ggcaagttta
     2881 caaatacaca accaatcccg atgtggcaac aggcgatggc gtggcaatgg gaatcaagtg
     2941 tggtgcaatg gtatctaatt tgaattttat ccagttccac cctactgctt tgtacgaaga
     3001 aaataaacct cgtttggatt tgttaaccga ggcaatcaga gggtttggtg cccacatcgt
     3061 taataaaaac ggtaaacgct ttgtgtttga ttacgataac cgaggcgaat tggcaacgcg
     3121 cgatattgtt tcgaaagcaa tttttagcga actcagaaaa agtgatgaaa aatgtgtgta
     3181 tttggattgc aaacatttgg ataaagataa attcaacgaa aaattcccac aaattgcagc
     3241 taatttacaa tcgaaaaact tagatattgc aattgattta attccggttg taccagcaac
     3301 acactatcaa tgtggcggat taaaagttga cgttgatggt caaacaacag ttacaggttt
     3361 atttgccatt ggcgaatgtg cagaaacagg cttgcacggt gccaatcgtt tagcttccaa
     3421 ctcgctttta gaaggattgg tttttgcaca tcaagcagcc aatttcattg cagaaaatta
     3481 cgagcaattt gtgagcgatt catcgataga atctccaact tataccctcg ataaaagcaa
     3541 tgatgctgtt tttgaaactg ctattgacca gttgagaaat accatgtcaa aatatgcaac
     3601 catcgcttca agtttagacg acatagagct atgcatgcaa cagttggatg ctatcaataa
     3661 ttctattgaa tccatcaaaa acaatcaaat catttcagag caacgattga ttttcaacaa
     3721 cttaatggtg aatgcacgtg caattgcaaa atttaaacgc ttacacattt tgcatgctca
     3781 aaaaactgaa acattagaga aacactaact ataaaacaaa aactatggaa aacaagaaac
     3841 aaaatcgctt gcgtacttta acagaaccgt tcaaaataaa agtagttgaa cccttaaaag
     3901 tgactgccga agaataccgt gaagaagcat tggaaaaagc aggatacaat ccgtttttat
     3961 tgagttcaaa agacgttttt atcgacttat tgaccgatag tggaacgggt gctatgagcg
     4021 ataaccaatg gggagccatc atggtaggtg atgaaagcta tgccggggcg agaagctggg
     4081 aaaaattgga aaatgtagta aaagatttga caggaatgcc ttatgtttta cccactcacc
     4141 aaggtcgcgc agcagaacgt attttgtatt ctaccatagg aggtcccaac aaagtgttta
     4201 tcagcaatac ccattttgat actacacgtg ctaacattga gtttgtaggt tcaacagcta
     4261 ttgacattgt ttgccctgaa gcatttgaca atcaatcgga ttatccattt aaaggcaaca
     4321 tcgatttgca aaaattagaa gccttaattg cgcaatatgg aaaagaaaac atagcggctg
     4381 tgattatgac tgtaaccaac aattcatcgg gcggacaacc tgtaagcatg gaaaacttca
     4441 aagcagtacg taaaatttgt gatgcaaacg gaattttctt ggtgatggac ggttgtagaa
     4501 ttgccgaaaa cagttatttc gttcaccaca aagaagctgc ttgttccaat aaaacctacc
     4561 gtgaaattgc acaagaaatg tttgcgcttt ccgatgcatt tgtgatgagt gccaaaaaag
     4621 atggaatggt aaacatgggt ggtttgctca ccttgcgcga tgaagcattg tcggttaaat
     4681 gtaaaaactt actcatcatt tccgaaggat ttacaaccta tggtggtttg tcggggcgtg
     4741 atatggaagc acttgccgtt ggtttggtag aagtttttga tgaagattat ttgcactacc
     4801 gtatcaagag taccacctat ttaggcgaag gattgcgcaa aaaaggaatt ccagttgtgt
     4861 ggccaattgg cggacatgcg gtgtacatcg atgcaaaaga attgtataaa aatattccgt
     4921 taaaagaata ccctggtcag gcgttggttt gtgatttgta ccttaaaggt ggcgttagaa
     4981 ctgtggaagt tgggagcgta atgtttggta aatacgatga aaacggcgaa ctcattccag
     5041 cagaaaaaga attggtgcgt ttggctattc cacgtcgtgt ttatacccaa agtcacattg
     5101 attatgtact tgatgttttt gatgacatct tggaaatgag agatagaaaa aatggttatg
     5161 aaattacgta tgaacctcca ttcttgcgcc atttcaccgc acatttcaaa ccaataaaca
     5221 agtaaaatta aactctataa tatggctaaa ttagaaaaaa gatcgtgggc agaaccatac
     5281 agaatgaaaa tggtggagct cctcaaaatg acaaccaaag cacaacgcga aaaagcatta
     5341 aaagaagctg gatacaatac ttttttattg cacgccgaag atgtgtacat tgacttgtta
     5401 accgatagtg gaacaaacgc catgagtgac cgtcaatgga gtgcatttat gactggtgat
     5461 gaggcctatg ccggaagtaa aagtttttac ttaatggaaa aagcgattca acagttttat
     5521 ggttacaaat acgttattcc aacgcaccaa ggaaggggcg ctgagaactt gttatcgcaa
     5581 attttaatta aaaaaggtga ttttgtacca ggaaacatgt atttcaccac tactcgcttg
     5641 caccaagaat taaacggagc aacatttgtg gatattattg ttgacgaggc acacgaccca
     5701 caagatgaat caccatttaa aggaaatgtg gacatcagca aattggatgc attggtgaaa
     5761 aaagaagggg ctaaaaaaat tccttatgta tgtattgcta ccaatgtgaa tatggcgggt
     5821 ggacaaccaa tcagcattaa aaacttaaaa gaattgcgtg aatataccaa caaacacggt
     5881 attaaaatta ttcacgatat gacacgtgtt gccgaaaatg catatttcat tcaacaacga
     5941 gaagaagggt ataaaaacgt atccatcaaa gacattgtta aagaaatttg ttcgttaacc
     6001 gatggagcaa ccatgagtgc taaaaaagat gctttggtaa acattggtgg ttttatggct
     6061 accaacgatt gggatgtgta tgaagaagca cgcaacttgg tt
//
LOCUS       DAOQVQ010000100         5981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14640_length_5981_cov_3.668748, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000100 DAOQVQ010000000
VERSION     DAOQVQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_5981_cov_3.668748"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PKN22_04435"
     CDS             complement(<1..1047)
                     /locus_tag="PKN22_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS41984.1"
                     /translation="MTRILFILDASNSMNALWDGKETRFAAAKRILSKSVMDLQGVGN
                     LEVALRVYGHQSEVTATYQDCDDTKLEIPFGVNNHDRIINRIKTIQAKGTTPIARSLE
                     AAAGDFPDANSRNVIILITDGLEACDNDPCIIAKKLHDKGVNVKPFVIGVGMDLSYLE
                     KFRCIGEYADAETPESFEKVLQNVVNKALVNTTVQINLNNINKEPKETDVTLFLYKAG
                     TKELKYTFTHTLNRSGLPDTLVMDPAIDYDMVVNTLPKREVKGIHLKKHTHNTIIADV
                     PQGYIQTKVVLGNRQYVIPTRVMLKGNPATINVQQVDETDKYLIGTYDLEILTLPRTY
                     ATVEVTQSSVTKVDI"
     gene            complement(1115..1957)
                     /locus_tag="PKN22_04440"
     CDS             complement(1115..1957)
                     /locus_tag="PKN22_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNS41985.1"
                     /translation="MENTELKRIASQVRRDIVRMVAAVSSGHPGGSLGCADFFTLMYF
                     EVMKQDPKNFTMDGKNEDVFFLSNGHISPVWYSVLARAGYFPVNELNTFRKISSRLQG
                     HPSTEKGLEGIRMASGSLGQGLSNAIGTAQVKKLNNDPCLVYSLHGDGELQEGQIWEA
                     AMYAAAKKVDNLIATVDYNGRQIDGDCEDVVSLGDLKGKWQAFGWEVLEMDGHNFDDM
                     RSTLAQAKAKTGQGKPIVILMKTEMGQGVDYMMGTHKWHGSAPNAEQLEKALNQLEET
                     IGDY"
     gene            complement(2125..4341)
                     /locus_tag="PKN22_04445"
     CDS             complement(2125..4341)
                     /locus_tag="PKN22_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS41986.1"
                     /translation="MKKVVSAFSVVAFLGSVSFAQEVNIKNLRQLTNGGDNAEAYFSP
                     DGTKLTMQVTNPKEGVECDQIYELDLSIQNPTFKDLKLISTGTGRTTCSFFMPDGKHI
                     LYASTHEGGHACPAPPKANGKYLWPIFPEFDIYVADLNGKIVNQLTNTPGYDAEAVLS
                     PDGKKIAFTSMRSGDLEIYTMDLDGSNVKQITFGLGYDGGAFFSHDSKKLVFRSSRPK
                     TEEEIKEYKDLLSQGLVAPTNMEIYTVNVDGTDLKQVTNLGKANWSPYFHPSDKSIIF
                     SSNHHSVHGYDFQLFMIDADGTNLRQITFESNFNAFPMFSPDGKKLVYASNRIPDKPK
                     ETNVYIADWVETDAAEVVQDKQLKKHVSTLASDKMEGRLTGSKGEQKAAKYISSELKK
                     YGLKPYEGKSYLQKFNYKVRLNPHDSASTKDWHGTNVIGVLDNGAKNTIVIGAHYDHL
                     GRNEHHHSTKVNSEGEIHNGADDNASGTAALLELARMYATNSTKEKVNYVFAFFSGEE
                     DGLIGSKHMAESLKSKFPNVLTMINMDMIGRLSKDKALVIGGVGSSPDFGPIIDKTKP
                     AGFTITIDSTGVGPSDHTSFYLKDIPVLFFFTGTHMDYHKPSDDEEKINYYGLRAITG
                     FAARVANQIAEKGQMTFTKTKVETGKRTPKYKVTLGVMPDYTDHGDGLHIDGVVDGKA
                     AANAGVVAGDILIAIGDCTIKDVYGYMECLSKLQAGDERELTVIRQGKEVKLKAAF"
     gene            4553..5572
                     /locus_tag="PKN22_04450"
     CDS             4553..5572
                     /locus_tag="PKN22_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HNS41987.1"
                     /translation="MTETNYKQTESRKDHHLELALSSQNELVDSRFFYEPLLAAHPSD
                     SEEWPVKIGSKVMRFPIWISSMTGGTAKTNEINMRLAKTAATFGLGIGLGSSRIALEN
                     EKKIQDFDIRPIIGRQLPFYLNFGIAQIEKMLEQNSVAALEDLVAKLDADGIFIHVNP
                     LQEWMQPEGDRLKKTPIDTIQQFMATSKLAVIAKEVGQGFGYNSMKALMQLPLTAIEF
                     AANGGTNFSKLELLRNTTKSQFLMPFVHVGQTAEEMVDINNQLLAELGNKAQCQSVII
                     SGGIKSFLDGYYLLKKSKANAVYGQASEFLKYAQISQEALDEFTKNQVEGLLLARTYL
                     TLKQQ"
     gene            5576..>5981
                     /locus_tag="PKN22_04455"
     CDS             5576..>5981
                     /locus_tag="PKN22_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNS41988.1"
                     /translation="MHKDQHFVPGFSKKTRDEKLNYVVDNHLSSNEALELLKSFHHQD
                     AKIQNLLEEFSENTITNFPLPFNICPNVLINGKSYMVPMVVEESSVVAAASNSAKFWF
                     TRGGFKTEIIGTGKIGQVHFLWNDDVQLLINRF"
BASE COUNT         1760 a         1196 c         1045 g         1980 t
ORIGIN      
        1 aatatccacc ttggtaacac tgctttgggt tacctcaact gtggcgtaag tgcgtggcaa
       61 ggtgaggatt tccaaatcat aggttccaat taaatattta tccgtttcat ctacttgttg
      121 cacattgatg gttgcaggat ttcctttgag catcacacgg gttggaataa catattggcg
      181 attgcctaac accacttttg tttggatgta accttgcgga acatcggcaa taatggtatt
      241 gtgtgtatgc tttttaaggt gaatgccttt aacctcgcgc tttggaagtg tattgacaac
      301 catatcgtag tcgattgcag gatccatcac aagtgtatct ggcaatcccg agcgattcaa
      361 agtatgggta aacgtgtatt tcaattcttt cgtacctgcc ttgtataaaa acaacgtaac
      421 atccgtttct ttaggttcct tgttgatatt attcaaattg atttgaacag tggtattcac
      481 caatgctttg ttgaccacat tttgcaatac tttttcaaaa ctttcgggtg tttcagcatc
      541 ggcatattct cctatgcaac ggaatttttc caaatacgat aaatccattc ccacaccaat
      601 aacaaacggt tttacgttaa cacctttatc gtgcaatttt ttagcaataa tacacggatc
      661 gttatcacaa gcctctaaac catcagtaat taaaataatc acgtttctgg aattagcatc
      721 tggaaaatct ccagcagctg cttccaacga acgggcaatg ggcgttgtgc cttttgcttg
      781 aatggttttt atacgattga tgatgcggtc gtggttgttc actccaaatg gaatttccaa
      841 tttggtatcg tcacaatctt gataagttgc tgtaacttca gattgatgac cataaacccg
      901 caaagcaact tcaaggttgc ctacaccttg caaatccata accgacttgg ataaaatacg
      961 tttagcagca gcaaaacgtg tttcttttcc gtcccacaag gcgttcatac tgttagaggc
     1021 atccaaaata aaaagtatgc gcgtcaaacg ctcctgtcca aaagttaatg aactaaataa
     1081 taaaaatagt ataaggcaac ttattcgcat cgatttaata atctccgatg gtctcttcca
     1141 attgattcaa tgctttttcc aattgttccg cattgggagc agaaccgtgc catttgtgag
     1201 ttcccatcat gtaatcaacc ccttgaccca tttcggtttt catcaaaata acaattggtt
     1261 taccttgccc tgtttttgct tttgcttgtg ccaatgttga acgcatatcg tcaaaattat
     1321 gaccatccat ttccaagact tcccatccaa aagcttgcca ttttcctttt aggtcgccca
     1381 agctaactac atcctcgcaa tcgccatcaa tttgacgacc gttgtagtca accgttgcaa
     1441 ttaaattatc cactttttta gcggctgcat acattgctgc ttcccaaatt tgaccttctt
     1501 gcaattcacc atctccgtgt aaactataaa ccaaacaagg atcgttgttt aattttttca
     1561 cttgcgctgt tccgatagca ttagacaaac cttgtcccaa agaaccagag gccatgcgaa
     1621 ttccttccaa tcctttttct gtagaagggt gtccttgcaa acgggatgaa attttacgaa
     1681 atgtattcaa ttcatttact ggaaaataac ctgctcttgc caaaacgctg taccaaaccg
     1741 gactaatatg cccatttgac aagaagaata catcttcatt ttttccatcc attgtgaagt
     1801 ttttaggatc ttgtttcatc acttcaaaat acatcaatgt gaaaaaatcg gcacaaccca
     1861 atgaacctcc tgggtgtcca gagcttactg ctgctaccat tctaacgatg tctcttctaa
     1921 cttgcgatgc aattcttttt aattcggtat tttccattct tgtaaaattt atgcaaaatt
     1981 acattttttt aggcgatttt ttaagaatac taaaacgagt tttcaacgga tgttaatttc
     2041 aaccaaaaat aaaggaaaaa aaaagccccg ttcaaaaatg aacgaggccc ttttatgaac
     2101 actactttat ttatatatgt ttacctaaaa cgcagctttt aatttaactt ctttaccttg
     2161 acgaattacg gttaattcac gttcgtcacc agcttgaagt ttggataaac attccatgta
     2221 tccatacaca tctttgattg tacaatctcc gattgcaatt agaatgtcac ccgctacaac
     2281 accagcattt gctgctgctt ttccatctac aacaccgtca atatgcaagc catctccgtg
     2341 gtcggtataa tctggcatca cacccaacgt aactttgtat tttggcgttc ttttacctgt
     2401 ttctaccttt gtttttgtaa atgtcatttg tcctttttct gcaatttggt tggcaactct
     2461 tgctgcaaat cctgtaattg cacgcaaacc ataatagtta attttttctt catcatcact
     2521 tggtttgtgg taatccatgt gtgtacctgt aaagaagaac aacactggaa tgtctttcaa
     2581 ataaaacgaa gtatggtcag atggtccaac gccagtagaa tcaattgtaa tggtaaatcc
     2641 agcaggtttt gttttatcaa tgattggtcc gaaatctgga cttgaaccta ctccaccaat
     2701 aaccaatgct ttgtctttgc tcaaacgtcc aatcatatcc atgttaatca ttgtcaacac
     2761 atttggaaat ttgcttttca acgattctgc catgtgttta gaaccaatca acccatcttc
     2821 ttcacctgaa aagaatgcaa acacatagtt tactttttct tttgtactgt tggttgcata
     2881 cattcttgct aattctaaca acgccgctgt accagaagca ttgtcatctg caccattgtg
     2941 aatttcacct tctgaattca cttttgtaga atggtggtgt tcgtttctac ctaagtggtc
     3001 ataatgtgca ccaattacaa tcgtattttt agcaccatta tccaaaactc caatcacatt
     3061 tgttccgtgc caatcttttg tgcttgcact atcgtgtgga tttaaacgaa ccttgtagtt
     3121 gaatttttgc aaatagcttt ttccttcata cggttttaaa ccatattttt tcaattcaga
     3181 agaaatgtat ttagctgctt tttgttctcc ttttgagcct gttaaacgtc cttccatttt
     3241 gtcagaagcc aatgtagaaa cgtgtttttt caattgttta tcttgcacaa cttcagcagc
     3301 atctgtttct acccaatcag caatgtaaac atttgtttct tttggtttat ctggaattct
     3361 attacttgca tacaccaatt tttttccatc tggtgagaac attggaaaag cattgaaatt
     3421 actttcaaat gtaatttgac gtaggtttgt tccatcagca tcgatcataa acaattggaa
     3481 atcgtaaccg tgcacagaat ggtggttgga tgaaaaaatg atgcttttat cagatgggtg
     3541 aaaataaggc gaccagttag cttttcctaa attagtaact tgttttaaat cagttccgtc
     3601 tacgttaact gtataaattt ccatgttcgt tggagcaact aaaccttggc tcaacaaatc
     3661 tttgtattct ttaatttctt cttctgtttt tgggcgcgaa gaacggaata ctaatttttt
     3721 gctatcgtga gagaaaaaag cccctccgtc ataacccaat ccaaatgtga tttgttttac
     3781 gtttgaacca tccaagtcca ttgtgtaaat ttccaaatca ccagaacgca tacttgtaaa
     3841 tgctattttt ttaccatctg gagataaaac agcttctgca tcataacctg gtgtgttggt
     3901 taattggttg acaattttac cattcaaatc tgctacataa atgtcaaatt cagggaaaat
     3961 tggccataaa tacttaccgt ttgcctttgg tggagctgga catgcgtgtc ccccttcgtg
     4021 tgttgaggca tacaaaatat gtttgccatc tggcatgaag aaagagcaag ttgttctacc
     4081 tgtaccagtt gaaattaatt ttaaatcttt aaatgtcgga ttttgaatac ttaagtccaa
     4141 ttcataaatc tgatcgcatt ctactccttc ttttggattg gtaacttgca tggttaattt
     4201 tgttccatcc ggactaaaat aagcctctgc attatctcct ccattggtta attgacgtag
     4261 gtttttgata ttaacttctt gggcaaaact aacacttccc aaaaatgcaa ctactgaaaa
     4321 tgcacttaca actttcttca tttctgtttg attataaatt aaagtgcgac aaaagtagat
     4381 agaacaaatt ggagaaaagt cataagattg tcataaaagt gaattttatc ctattttgtt
     4441 tattttttgg agatttcttg tttctctgta aattctaaaa gcatctcgtg cataaattcg
     4501 atatttaacc gtaactttgt gttttgcttt tttattacat agtattgttg tcatgaccga
     4561 aacgaattac aagcaaacag aatcacgcaa agaccatcat ttggagttgg cgctatcttc
     4621 tcaaaatgaa ttggttgatt cccgtttttt ttatgaacct ttattggctg ctcacccttc
     4681 tgattcggaa gaatggccgg ttaaaatagg ttctaaagta atgcgttttc caatttggat
     4741 ttcaagcatg acagggggca cagcaaaaac caacgaaatc aacatgcgtt tagcaaaaac
     4801 agcagccaca tttggtttgg gaattggttt gggttcatcg cgcattgcgt tggaaaacga
     4861 aaaaaagatt caagattttg acattcgtcc aattattggt cgccaattgc cattttacct
     4921 caactttggt attgctcaaa ttgaaaaaat gttggaacaa aattcggttg ctgccctaga
     4981 agatttggtt gcaaaattgg atgccgacgg tatttttatt catgtaaacc cgttgcaaga
     5041 atggatgcaa cccgaaggtg atagactaaa aaaaacaccc atcgacacta ttcaacaatt
     5101 tatggcaacg tctaaacttg cagtaattgc aaaagaagtg gggcaaggtt ttggttacaa
     5161 cagtatgaaa gcgttgatgc aattgccatt aacagccatt gagtttgctg caaatggtgg
     5221 aaccaatttt tcaaaattag aattgttgcg caatacaaca aaaagtcagt ttttaatgcc
     5281 ttttgttcat gtgggacaaa cagcagaaga aatggtggat atcaacaatc aacttttagc
     5341 cgagctcggc aataaagcac agtgccaatc cgttatcatc agcggtggca tcaagagctt
     5401 tttggatggc tattatttat taaaaaaatc caaagccaat gcggtttatg ggcaagcttc
     5461 agagttttta aaatacgctc aaatttcgca agaggcactt gatgaattta ctaaaaatca
     5521 agtggaaggg ctgctattgg cgcgcacata tttaacacta aaacaacaat aacacatgca
     5581 taaggatcaa cattttgtac ctggtttttc aaaaaaaaca cgcgatgaaa aattgaacta
     5641 cgtggtggat aatcacctta gctcaaatga ggctttagaa cttttaaaga gttttcatca
     5701 ccaagatgca aaaattcaga atttattgga agaatttagt gaaaacacca tcaccaattt
     5761 cccgttgccg ttcaatattt gtcccaacgt gcttatcaat ggcaaaagct acatggtgcc
     5821 aatggttgtg gaggaaagtt cggtggtggc tgcggcttct aatagcgcta aattttggtt
     5881 cacgcgcgga ggttttaaaa cagaaatcat tggaacagga aaaattggtc aggttcattt
     5941 tttgtggaac gacgatgtgc aacttttaat caatcgtttt g
//
LOCUS       DAOQVQ010000101         5976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14656_length_5976_cov_3.953724, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000101 DAOQVQ010000000
VERSION     DAOQVQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14656_length_5976_cov_3.953724"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PKN22_04460"
     CDS             <1..958
                     /locus_tag="PKN22_04460"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNS41989.1"
                     /translation="ITQINLVFTAYFQGIKHFKIVNQILMLNGFYSLIVFAIAYLLNR
                     FEVYSVDLIDVILLSILVLLMNTAHWLYYYVRLNKNLFRFNLHFGKDLKIFLKFTGLN
                     HFANILIFLNQRMIIWFIAFYLDNWHLGVFSVGIGLAQLLYFFSSPISLILESFLSAQ
                     NQEKQNETFAIFSRLMFTIVLLICIVVLLVSSYVIPWLYGKEFYDSAVILNVVIFGIL
                     LSSHSSLLSSLFLAKDKLKYNVISSVFGVLTTLITAPIFIQKYQLMGAAYAQLATYTI
                     IFTVQYYFVNKHLFGNTNIFFLTRKDIRFLKKQLLLAKEERK"
     gene            complement(963..2621)
                     /gene="menD"
                     /locus_tag="PKN22_04465"
     CDS             complement(963..2621)
                     /gene="menD"
                     /locus_tag="PKN22_04465"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNS41990.1"
                     /translation="MVQTFVTACVKAGMETVVCSPGSRNSSLVIAFDEHPAINCYVIH
                     DERSAAFVAMGMALESGKPVGAVCTSGSAMLNYYPAVAEAYYQRIPLVIISADRPEKW
                     INQGDGQTIVQKGVYTNHIEGEAHFDESTDLQMVENTTIDLFSKITLHSKGPIHFNIA
                     LEEPLYQTVEITDELPQKIDWVKERTPLNNEEMEVLKKGLELPKKMILVGQMPQNPYF
                     LETLKTLANDTSFAILVENTSNLVSMKWVHCIDRTLQTIHPEEEANFAPDLLITFGGA
                     IVSKKIKAYLRKYKPQMHWKVGIDFPEMNTYESLTHSFQVSEASFVDTLNRIKSDLIP
                     SNYGSKWKQRDFIAQEKAEQFLETAPFSDLKAIELSLDCIPEGSHIHMANSSIVRYCQ
                     LFNPLQSMTYFSNRGTSGIDGSVSTAVGAALAKPSNLHVLLTGDVSFFYDSNALWNNY
                     TVSNLRIILINNGGGGIFNIIKGPRDSKQNARYFDAKHQFNAQYLAKTFGVEYFSARS
                     EEEMIDCMADFYNYTENSGIKLLEIHTQDIENHLVLDRYFKAIG"
     gene            complement(2701..3132)
                     /locus_tag="PKN22_04470"
     CDS             complement(2701..3132)
                     /locus_tag="PKN22_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNS41991.1"
                     /translation="MYLFLNDVSGSEIFVILIFILIFFGSKSIPGIARTLGRTMRQVK
                     DATSEIQNELKKSTDGYKKDLNLEGIFRDTAEEIKQPLDQMAVDLDHSIRYNPNSKLA
                     VNQPAQVAENEATSTVSEENPTEKVETTPPTPPTENQEKTV"
     gene            complement(3303..5336)
                     /locus_tag="PKN22_04475"
     CDS             complement(3303..5336)
                     /locus_tag="PKN22_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNS41992.1"
                     /translation="MKRIYLPVGIFVLGMALFSCSQKATDKNTENMEIVSEIGDIKVD
                     RFADVQVLRYEIKDFDKLTLNQKKLVYYLSQAGLAGRDINYDQNNPYNIRIRKALEAI
                     FENYKGERTGDDWNNFEVYAKQVWFSNGIHHHYGMDKFNPKFSKDYFQSLLKAVNHTL
                     DAATLEVMFNPEIAKSRKIKDPKQDIIVASANGFYGDGITLKMVEDFYANVKSPDPKR
                     PLEIGLNSTLILNNGKVQEDVWKSGGKYGKAIEQVIFWLKKATTVAENAQQKAALEKL
                     IEYYQTGDLKTWDDYNILWAQSTKGDIDWINGFIEVYGDALGMRANFESIVQITDFEA
                     SKQMKALAENAQWFEDNSTIMDEHKKKKVKGVSYKVVQAASESGDASPSTPIGVNLPN
                     NNWIREEYGSKSVSLGNIIEAYDKAGGKGMIDEFGYSKEEKERAQKHAYLGGKLHTAL
                     HEVIGHASGVINPGVGQPSESLKQYASTLEEARADLVGLYYIYDQKMIDLGLIPSFEV
                     AKAEYDGYIRNGMMTQLQRLELGQQLTEEHMQNRQLVASWAYEKGQKDKVIEKIVKDG
                     KTYFVVNDYLKLRDLFGQLLREIQRIKSEGDFKAGQALVDNYGVKVDRALHQEVLTRV
                     KPLNIAPYSGFVNPTLVPIRDNNGEIIDVKVSYDMNFVEQMLHYGKNYSFLEK"
     gene            5469..>5976
                     /locus_tag="PKN22_04480"
     CDS             5469..>5976
                     /locus_tag="PKN22_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS41993.1"
                     /translation="MSEVINDFGIQQILEELGIEAVNNGASTGGRWFNTRGEKIASYS
                     PVDNSLISTVDSATEVDYEAIVLKAQEAFEEWRMWPAPKRGEVVRQFGEKLREYKTAL
                     GTLVSYEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLTMHSERPGHRMYEQYHPLG
                     VVGIISAFN"
BASE COUNT         1723 a         1127 c         1036 g         2090 t
ORIGIN      
        1 tatcactcaa atcaaccttg tttttacagc ttattttcag ggtattaaac attttaaaat
       61 tgtcaatcaa attttaatgc ttaatggttt ttacagttta attgtatttg ctatagctta
      121 tttgttgaac cgctttgagg tgtattcggt tgatttgata gatgttatcc tattgtccat
      181 cttagtgctg ttgatgaata ctgcacattg gttgtattat tatgtgcggt tgaacaaaaa
      241 tctttttcgt tttaacctgc actttggaaa ggatttaaag atttttttaa aatttacagg
      301 actcaatcac tttgcaaata ttttaatctt tctcaaccaa cgcatgatta tttggttcat
      361 tgcattttat ttagataatt ggcatttggg agttttttcg gttggtattg gattggctca
      421 attgctttac tttttttcat cgcccatttc attgatttta gaatcgtttt taagtgctca
      481 aaatcaagaa aaacaaaacg aaacatttgc cattttctcc cgcttgatgt ttaccatcgt
      541 attgttgatt tgcatagttg ttttactagt gagtagttat gttattccat ggctctatgg
      601 aaaagaattt tacgattcgg cagttatttt aaatgttgtc atctttggaa ttttgctatc
      661 cagtcattca tcgttattgt cgagtttatt tttagcaaaa gataaattaa aatacaacgt
      721 catttcctct gtttttggcg tgctaactac attaattaca gctccaattt ttatccaaaa
      781 ataccaattg atgggtgctg cctatgcgca attagcaacc tacaccatta tttttacagt
      841 gcagtattat tttgtaaaca agcatctttt tggaaacaca aacattttct ttttaaccag
      901 aaaagatatt cgttttctta agaaacaact gttacttgca aaggaagaac gtaaataagc
      961 cgttaaccaa ttgctttaaa atagcggtct aataccaaat gattttcgat gtcttgggtg
     1021 tgaatttcca ataattttat tccagaattt tctgtataat tgtaaaaatc tgccatgcaa
     1081 tcaatcattt cctcttctga acgtgcagaa aaatattcta caccaaaagt tttagcaagg
     1141 tattgggcat taaactgatg tttggcatca aaataacgag cattttgttt tgaatcgcgt
     1201 ggacctttga taatattaaa aattccacct cccccgttgt ttatgagaat aattcgtaaa
     1261 ttgcttactg tataattgtt ccacaaagca ttgctgtcgt aaaaaaacga tacatcgccc
     1321 gttaacaaca catgtaaatt ggaaggtttt gcaagtgctg caccaactgc tgtagaaaca
     1381 gaaccgtcaa ttcccgaagt tcctctgttg gagaaatagg tcatgctttg caacggatta
     1441 aacaactgac aataacgcac aatggaacta tttgccatgt gaatgtggga accttctgga
     1501 atgcaatcca acgacaattc aatggctttt aaatctgaaa aaggtgcagt ttccaagaat
     1561 tgctcagctt tttcttgcgc tataaaatca cgttgtttcc atttgcttcc atagttggac
     1621 ggaattaagt cgctttttat tcggttcaat gtgtccacaa aactggcttc gctcacttga
     1681 aacgagtggg taagggattc gtaagtgttc atttctggaa aatcaattcc aaccttccaa
     1741 tgcatttgtg gtttgtattt acgcaaataa gccttaatct ttttagaaac aatcgctcca
     1801 ccaaaggtaa tgagtaaatc gggtgcaaaa ttagcttctt cttctggatg aatggtttgc
     1861 aaagtgcgat caatgcaatg tacccatttc atggaaacta aattggatgt gttttcaacc
     1921 aaaattgcaa aagaagtatc gtttgccaat gtttttaaag tttctaaaaa atagggattt
     1981 tgtggcattt gtcccactaa aatcattttt ttaggcaatt ctaatccttt tttgagcacc
     2041 tccatttctt cattgttcaa cggagttctt tcttttaccc aatctatttt ttgaggaagt
     2101 tcatctgtta tttcaactgt ttgatacaac ggttcttcca atgcaatgtt gaaatgaata
     2161 ggaccttttg aatgcaatgt aatttttgaa aacaaatcaa tggtggtgtt ttcaaccatt
     2221 tgcagatcgg ttgattcgtc aaaatgtgct tcaccctcga tatgattggt ataaactcct
     2281 ttttgaacga tggtttgtcc atctccctga ttgatccatt tttcgggtct atctgccgaa
     2341 ataattacca acggaatgcg ttgataataa gcctctgcaa cagctggata ataattcaac
     2401 atggcagaac ccgaggtgca aactgctcca accggttttc cagattccaa agccatgccc
     2461 attgctacaa atgcggccga gcgttcgtcg tgaattacgt aacagttaat tgcaggatgt
     2521 tcgtcgaatg ctatgaccaa cgatgaattg cgagaaccgg gagaacaaac aactgtttcc
     2581 atacctgctt ttacgcaagc ggtaacaaat gtctgtacca tttttttgtc ggatgaaatc
     2641 atggttcaaa tttagctttt tcgctaatat tggtcaacga taaacagatt ttgtttgcta
     2701 ttaaactgtc ttttcttggt tttcagttgg tggtgttgga ggtgttgttt ctactttctc
     2761 ggttggattt tcttcactaa cagttgaagt agcttcattt tctgcaactt gcgcaggttg
     2821 gttaactgct aattttgaat tggggttgta acgaatggaa tgatctaaat ccacagccat
     2881 ttggtcaaga ggttgtttaa tttcttctgc tgtatctcta aaaatccctt ctaagttcaa
     2941 atcttttttg taaccgtcgg tagatttttt taattcattt tgaatttcag aagtagcatc
     3001 tttcacttgg cgcatggttc tacccaatgt gcgcgcaata cccggaatgc tctttgaacc
     3061 aaagaaaatt aaaatgaaaa ttagaattac aaaaatttca gaacctgata cgtcgtttaa
     3121 aaataaatac acttctttta ttttttttca aaattaagat tttttacgca gatgttgagc
     3181 tgatttgtta aaattaacgt ttgttgggtg atatcgacat atcgacatag ttcgatatca
     3241 tcaaaaatag caacaaaaaa gccgaagttt taacgctccg gcttcctgtt tatttcactt
     3301 ttttatttct ccaaaaaact atagttctta ccataatgta gcatttgctc cacaaaattc
     3361 atatcgtaag aaactttcac atcaatgatt tcaccgttgt tatcccttat tggaaccaat
     3421 gttgggttca caaaaccaga atagggcgca atgtttaacg gtttcacacg cgtcaatact
     3481 tcttggtgca aagctctgtc cacttttaca ccgtagttat ccaccaatgc ttgccccgct
     3541 ttaaaatcgc cttctgattt tatacgttga atttcgcgta acaattgccc aaataaatca
     3601 cgtaatttaa ggtaatcgtt caccacaaaa tacgtttttc cgtcttttac aattttttca
     3661 atgactttgt ctttctgccc tttttcatac gcccaacttg ccaccaattg acggttttgc
     3721 atgtgctctt cggttaattg ttgccccagt tccaaacgtt gcaattgcgt catcatccca
     3781 tttctgatgt agccgtcgta ttctgctttt gccacttcaa acgatggaat aagtcctaaa
     3841 tcaatcattt tttggtcgta gatataatac aatcccacca aatctgcacg tgcttcttcc
     3901 aaggtgcttg catattgttt gagtgattct gaaggctgac caacacctgg gttaattaca
     3961 ccagaagcgt gaccaataac ttcgtgcaat gccgtatgca atttgccgcc caaataagca
     4021 tgtttttgag cacgttcttt ttcttctttg gaataaccaa attcatctat cattcctttt
     4081 ccaccagctt tgtcataagc ctcaatgatg tttcctaaac tcaccgattt agagccgtat
     4141 tcttcacgaa tccagttgtt gttaggcaaa tttacaccaa ttggagtaga aggagaagca
     4201 tctcccgatt cactggctgc ttgcactact ttgtaactta caccttttac ttttttcttt
     4261 ttgtgctcat ccatgatggt tgaattgtcc tcaaaccatt gtgcattttc tgccaaagcc
     4321 ttcatttgct tagaagcctc aaaatcggta atttgaacta tgctttcaaa attggcacgc
     4381 attcccaatg catcgccata cacttcaata aaaccattaa tccagtcaat atcgcctttg
     4441 gtagattgcg cccacaaaat gttgtaatcg tcccacgttt ttaaatcacc tgtttggtaa
     4501 tattcaatca gtttttccaa ggctgccttt tgttgtgcat tttcggcaac agtggttgct
     4561 tttttcaacc aaaaaataac ttgttcaatt gctttaccat attttccgcc cgatttccaa
     4621 acatcttctt gcacttttcc attgtttaaa atcaacgttg aatttaatcc aatttcaagc
     4681 ggacgcttag ggtcgggact tttcacattg gcataaaaat cttctaccat ttttagggta
     4741 atgccatcgc cataaaaccc atttgccgaa gcaacaatga tgtcttgttt cggatcttta
     4801 atttttcttg atttagcaat ttcaggatta aacattactt ccaatgttgc tgcatcaagc
     4861 gtgtgattca cagcttttaa caacgattga aaataatctt ttgaaaattt cgggttgaac
     4921 ttatccatcc cataatggtg gtgaattccg tttgaaaacc atacttgttt ggcatatacc
     4981 tcaaagttat tccagtcatc gccagttctt tcgcctttgt agttttcaaa aatagcttcc
     5041 aatgctttgc ggatgcgtat gttgtaggga ttgttttgat cgtaattgat gtcccttccc
     5101 gccaatccag cttgagaaag atagtacacc aatttttttt ggttcaaggt taacttgtca
     5161 aaatctttta tttcgtagcg caatacttga acatcggcaa aacgatccac tttgatgtcg
     5221 ccaatttcac ttacaatttc catgttttct gtatttttat cggttgcttt ttgcgaacaa
     5281 ctaaataaag ccattcccaa aacaaaaatg cctactggca aatagatgcg tttcatagtt
     5341 ttattgattt agaaaacgta aatttaacaa actttttata gatgtgaaaa tcaaatgcag
     5401 ttgataagta tttgttcatt ttcttttgaa aatatattat atttgtgaaa tattaaaact
     5461 aagttaaaat gtcagaagtc ataaacgatt ttgggattca acaaatcctt gaagaattag
     5521 gaatagaagc agtaaacaat ggcgcttcaa caggcggcag atggttcaat acaagaggcg
     5581 aaaaaattgc atcttactca ccagtagata attcacttat ttcaacggtt gattctgcaa
     5641 cagaagttga ttacgaagca attgtattaa aagcacaaga ggcttttgaa gaatggagaa
     5701 tgtggcctgc accaaaaaga ggagaggttg ttcgtcagtt tggagaaaaa ttgcgcgagt
     5761 acaaaacagc tttaggaacg ttggtttctt acgaaatggg gaaatcgttg caagaaggat
     5821 tgggcgaagt acaagaaatg attgatatct gcgattttgc agttggttta tcacgtcagt
     5881 tgtatggttt aacgatgcat tcggaaagac ctggacacag aatgtatgag caataccatc
     5941 cgcttggagt tgtaggaatt atttctgcgt tcaact
//
LOCUS       DAOQVQ010000102         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14750_length_5945_cov_3.883701, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000102 DAOQVQ010000000
VERSION     DAOQVQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14750_length_5945_cov_3.883701"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2348)
                     /locus_tag="PKN22_04485"
     CDS             complement(93..2348)
                     /locus_tag="PKN22_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS41994.1"
                     /translation="MSGTIKDGENGEDIFDATVVVKELTNTGTKTNAYGFYSLTIPAG
                     TYTFIYRMAGYDQKEITIELTQNKQHNLELTPSKDIKNLQEVEVTAVKENDNITKSGM
                     AVTTFTPKDIETIPVLFGEKDIVKTLQLTPGVKAAGDGNAGFYVRGGGADQNLVLLDE
                     APVYNASHLLGFFSVFNSDAIKDVSLYKSGIPAQYGGRASSVLDVKMREGNNKRFNVS
                     GGIGLIASRLTVEGPIVKNKGSFIVSGRRTYMDLFLKASRKPELKSTKLYFYDLNLKA
                     NYNITSRDRLYLSGYFGRDNFSFNDEFGFKWGNGTGTIRWNRIVTEKMFSNTSFVFSN
                     FNYAFDITSGGSKLKVGASIQDLNLKQDFDYFINNSNSLKFGFNVIYHTFKPGELKST
                     MSAINNFKIDNQYALEMGVYIQNDQKIGNWLSIMYGVRYSGFDYMGKGIAYSYDEKGK
                     KTGEQAYGDFKTIKYHHFLEPRLSMSFILSEKNSLKIAYNRNAQFLHQLSNSSSSSPT
                     DIWVPSTNNVKPQIADQVAMGYYRNFLDNMLVLTTEVYYKHLGNQVDYRNGASLFLNN
                     EIEGGLVYGKGQTYGFELQFEKKKGKFTGWISYTLSRSLRKFKDINQGKQFPARQDRI
                     HDLSVVAMYRINQKFALSGTFVFYTGDAVTFPSGKYEINGAVVPYYTERNGYRMPNYH
                     RLDLGLTVYFKERPKFEHNLNVSIYNVYAHRNAFMITFEEDKDNPGKTKAIQTSLFRL
                     VPSITYNFKIK"
     gene            complement(2431..2910)
                     /locus_tag="PKN22_04490"
     CDS             complement(2431..2910)
                     /locus_tag="PKN22_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS41995.1"
                     /translation="MKTKNDVGYYAIGIFRGKTAHNIGTLWRSAYILGAQYIFTIENR
                     YKKQASDVVRAWSRIPLFHYDTFEDLLNNIPYDCRLVGVELTDDAEWLHEFNHPKRAI
                     YLLGSEDCGLPDFVKEKCHYLIKLPGNSSLNVGVTGGIVMHDRVSKMPTNLPPHHNE"
     gene            3060..4868
                     /gene="sppA"
                     /locus_tag="PKN22_04495"
     CDS             3060..4868
                     /gene="sppA"
                     /locus_tag="PKN22_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNS41996.1"
                     /translation="MKVKGKKISFGKVFWPSLVAVIIGSIVGMILFFVIAGGIANSLM
                     PTPTKVVAEPNSVLHIKLDGVVAEKTNSELDFASFNVTTNLGLADLLYGLEKAKKDNA
                     IKGVFLELGSLSCGYTTAKEIRKAINDFEKSGKFVVAYNAGEMVGLGKYYISSAANEL
                     YGFPTSIMEFLGLGGELTFYKHTLEKLGIEVQVIRGKNNDFKSAVEPFFLDKMSDSSR
                     LQMERYINNIWTEIRSDIAKDRKISPEELNNYAENMLIRRMKDAEKYKLIDATKYRDE
                     VIDIIKKKLKLAVDKDVKFVAFEKYAKSNFRDNQLLVKTGEPNVAVIIAAGDITVDGD
                     GLTSKETCKLLKEARNNKSIKTVVLRVNSPGGSALASDEIWREVKLTNKEKKVIVSMG
                     DVAASGGYYISTAASRIFAEPTTITGSIGVFGMVPYFGNFMKDKLGFSYDYVKTNQYA
                     GFTLNKKMTEKELSNVQQEVDEIYQDFLERVAEGRKMTVEQANVFARGRVWTGSDAIK
                     IGLVDELGGLKDAIDYAAKTAGVKDPKVLYYPLVKEDMFETIMEQLQEDMKKSNVSVK
                     GNIPTELLMHYQKIAKLEGMFGIQMRMPFVMDLELK"
     gene            complement(4982..5347)
                     /locus_tag="PKN22_04500"
     CDS             complement(4982..5347)
                     /locus_tag="PKN22_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS41997.1"
                     /translation="MDKLIGKKIRKVREIKNFSQEYVANKLSISQSAYSNIENGKTLI
                     SEKSLNSIAKILEVTPEIIQGYNDQVIFNSCIQSGYVNTNNINNPIDKIESLYEEIIK
                     QLKERIHILDEIIKLKNNT"
     gene            5471..5830
                     /locus_tag="PKN22_04505"
     CDS             5471..5830
                     /locus_tag="PKN22_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS41998.1"
                     /translation="MKKLIYFFLLVFIGTTLSCNKENIVKEVSTKERIFSEEEMVWVT
                     PNGYVIPATERNHWEKYIAQTFNEKAPKGYESSSCTTESIKCGLKCSASRLDNCSKEM
                     SCTPCMNCGCTPIKSPY"
BASE COUNT         1933 a          960 c         1085 g         1967 t
ORIGIN      
        1 actagcttcg tttaaatcaa catttatttc tttttggcaa cctacaacga ttaaacaaag
       61 aagtgcgaat aaaacagtaa ttttcataag gtctatttta ttttaaagtt ataagtaatg
      121 gaaggaacca aacggaaaag tgaggtttgg atagcctttg tttttcccgg attatccttg
      181 tcttcttcaa atgttatcat gaatgcattt ctgtgagcat acacgttgta aatagaaacg
      241 ttgaggttgt gttcaaattt tggtcgctct ttgaaataaa cagtcagccc caaatccaat
      301 cggtgataat tgggcattct gtagccattg cgctcggtgt agtagggcac aactgctccg
      361 ttgatttcat attttccaga tggaaacgta acagcatctc ccgtataaaa aacaaaggtg
      421 ccagaaagtg caaatttttg attgatgcgg tacatggcaa caactgataa atcatggatt
      481 ctatcctgac gtgctggaaa ttgcttgcct tggttgatgt ctttgaattt acgcaacgaa
      541 cgcgataggg tgtagctaat ccaaccggta aattttcctt ttttcttttc aaattgcaac
      601 tcaaaaccat aagtttgccc ttttccatac accaatccac cttcaatttc attgtttaaa
      661 aacaaactgg ctccgttacg atagtcaacc tgattgccca aatgtttgta ataaacttcg
      721 gtagttaaca ccaacatgtt gtccaaaaag ttgcggtaat aacccatcgc cacctgatcg
      781 gcaatttgag gttttacgtt gttggtgctc ggaacccaaa tatcggttgg actactagac
      841 gatgaattgg acaattgatg taaaaattgt gcatttctgt tgtaggcaat tttcaatgaa
      901 tttttttcag aaagaataaa gctcattgat aatcggggtt ctaaaaaatg gtggtatttg
      961 atggttttaa aatcgccgta tgcttgctcg cccgttttct tgcctttttc atcgtagcta
     1021 taggcaatac cttttcccat atagtcaaaa cccgaataac gcacaccata catgatggat
     1081 aaccaattgc caattttttg atcgttttga atgtaaacgc ccatttctag tgcatattga
     1141 ttgtcaattt taaagttatt gatggccgac atagttgatt tcaactcacc aggtttaaag
     1201 gtatgataaa tcacgttgaa cccaaattta agtgagttgc tattgttaat gaagtaatca
     1261 aaatcttgtt tgaggtttaa atcttgaatg gaagcgccaa ctttgagctt gctaccaccc
     1321 gatgtaatgt caaacgcata attgaagttg ctaaaaacaa acgaggtatt ggaaaacatt
     1381 ttctcggtta ctattctatt ccagcgtatg gttccagtgc catttcccca cttgaagcca
     1441 aattcatcgt tgaacgaaaa attatctctt ccaaaatacc ccgataaata aagacgatct
     1501 ctactggtaa tgttgtaatt agcctttaaa ttcaaatcgt aaaaatacaa ttttgtactt
     1561 ttgagctcag gtttacgaga tgcttttaga aacaaatcca tatacgttct tcgccccgaa
     1621 acaatgaaag aacctttgtt ttttacaatt ggcccttcta cagttagacg tgaggcaatt
     1681 aaaccaattc cgcccgaaac attgaaacgt ttgttgtttc cttcgcgcat tttaacatcc
     1741 aaaacagaag atgctcgccc tccatattga gctggaatac cagatttata caacgaaaca
     1801 tctttaatag catccgaatt aaacaccgag aaaaatccta ataaatgcga agcattgtac
     1861 accggagctt cgtccaacaa aactaaattt tgatcagccc caccaccacg cacataaaat
     1921 cctgcgttac catcgccagc ggcttttact ccaggggtta attgcaaggt ttttacaatg
     1981 tctttctcgc caaataaaac aggaatggtt tcaatatctt ttggtgtaaa cgttgtcact
     2041 gccatcccag atttggtgat attgtcgttt tcttttacag ccgttacttc tacttcttgg
     2101 agatttttga tgtctttgga gggtgttaac tcaaggttgt gctgcttgtt ttgtgtcaat
     2161 tcaattgtta tttctttttg gtcgtatcca gccatgcggt agataaatgt gtaggttccc
     2221 gctggaatgg tcaatgaata aaatccataa gcatttgttt ttgtgcccgt gttggttagt
     2281 tcttttacaa ccaccgttgc gtcaaaaata tcttcgccat tttcaccgtc ttttatcgtt
     2341 ccgctcaaag taaatttctg agcatggacc aatgaaaggc aacaaataga gaagcaaaaa
     2401 agagttagtt taattttcat aaatattttt ctattcgttg tgatgaggtg gtagatttgt
     2461 aggcattttg gaaaccctgt cgtgcattac aattccgcca gttacaccaa catttaatga
     2521 cgaatttccg ggtagtttaa taaggtaatg gcatttttct ttcacaaaat cgggaaggcc
     2581 gcaatcttca gaacccaata agtaaatggc tctttttggg tgattaaatt cgtgcaacca
     2641 ttctgcatca tcggtaagtt ctacaccaac tagtcgacaa tcgtagggaa tattgttgag
     2701 caaatcttca aacgtatcgt agtgaaacaa aggaatgcgc gaccaagcac gaacaacatc
     2761 cgaagcttgt tttttatacc gattttcgat ggtaaaaatg tattgagcac ctaaaatgta
     2821 ggcacttcgc cacaaggtac caatgttgtg tgcagttttt ccgcgaaaga ttccaatggc
     2881 ataatagcca acatcgtttt tggttttcat tgcaacaaat ataacaacta attgaatttc
     2941 atagatgttt gtaatcattg caatctacca tttaaactaa atttctaacc attcataaaa
     3001 atataacaac tgtttggctt ttttcctatt agttttgaag ttgaaaaata gtttaacaca
     3061 tgaaagtgaa aggaaaaaaa ataagtttcg gaaaagtatt ttggccaagt ttggttgcag
     3121 taatcatcgg atcaattgtt gggatgattc ttttctttgt tattgcaggt ggaattgcaa
     3181 acagtttgat gccgactcca accaaagtag tggcagaacc caattcggtt ttacacatta
     3241 aattggatgg cgttgtagca gaaaaaacca attcagaatt ggattttgct tctttcaatg
     3301 taaccacaaa tttaggatta gcagatttac tttacggttt agaaaaagct aaaaaagaca
     3361 atgccatcaa aggtgtgttt ttagaattag gaagtctttc atgtggttac acaacagcta
     3421 aagaaattcg caaggctatc aacgattttg agaaaagtgg aaaatttgtt gttgcctaca
     3481 atgctggtga aatggtagga ttaggaaaat actatatttc atctgctgct aatgaattgt
     3541 atggttttcc aacttcaatt atggaatttt tagggttagg tggtgaatta acgttctaca
     3601 aacatacgtt ggaaaaattg ggtattgaag tacaagtaat tagaggaaaa aacaacgatt
     3661 tcaaaagtgc tgttgaacca tttttcttag ataaaatgag cgattctagc cgtttgcaaa
     3721 tggaaagata catcaataat atttggacag aaatacgttc tgacattgct aaagatcgta
     3781 aaatcagccc tgaagaattg aacaattatg ctgaaaatat gcttatccgc agaatgaaag
     3841 atgctgaaaa gtataaattg attgatgcta ccaaataccg cgatgaagta attgacatca
     3901 tcaagaaaaa attaaaattg gcagttgata aagatgtgaa atttgttgct tttgaaaaat
     3961 atgctaaatc caattttaga gacaaccaac tattggttaa aacaggtgaa cctaatgttg
     4021 cagtgattat tgctgctggc gatattaccg ttgatggcga tggtttaaca tctaaagaaa
     4081 cttgcaaatt attaaaagaa gcacgcaaca ataaatctat aaaaacagtg gtgttgcgcg
     4141 taaattcacc aggaggaagt gccttggcaa gtgatgaaat ttggcgcgaa gtgaaattaa
     4201 ccaacaaaga aaagaaagta attgtatcaa tgggcgatgt ggctgcatcg ggtggatatt
     4261 acatctctac agcagcatct cgcatttttg ccgaaccaac taccatcaca ggttctatcg
     4321 gtgtgtttgg aatggtgcct tattttggaa atttcatgaa agacaaatta ggattctcgt
     4381 atgattatgt gaaaaccaac caatatgctg gttttacttt gaacaaaaaa atgaccgaaa
     4441 aagaattgag caatgtgcaa caagaagtgg atgaaattta tcaagatttc ttggagcgcg
     4501 tagctgaagg acgaaaaatg acggttgaac aagccaatgt gtttgcacgt ggacgtgttt
     4561 ggacaggatc ggatgctatt aaaattggtt tggtggatga gttaggaggt ttaaaagatg
     4621 caattgatta tgctgcaaaa acagcggggg ttaaagatcc taaagtattg tattatccac
     4681 ttgtaaaaga ggatatgttt gagactatca tggaacaatt acaagaagat atgaagaaat
     4741 caaatgtaag tgttaaagga aatattccta cagaattact aatgcactac caaaagattg
     4801 caaaattaga aggaatgttt ggaatccaaa tgcgtatgcc gtttgtgatg gatttggaat
     4861 tgaaataatc gaattgttta aatcataagc acagttaaat aattcatagt gaactgctcc
     4921 gagaaattgg agcagttttt tgtcatttga gtacttaaat cgctagttat tataatgata
     4981 attaagtatt attttttaat ttgattattt catcaagaat atgtattctc tcttttagtt
     5041 gttttattat ttcttcatat aaagattcaa ttttatcaat aggattgtta atattatttg
     5101 tatttacata acctgattga atacaagagt taaaaatcac ttgatcatta taaccttgaa
     5161 ttatttcagg tgttacttct aatattttcg caattgaatt taaacttttt tcagaaatta
     5221 aagttttgcc attttcaata tttgaatagg cgctctgact aatagataat ttattagcaa
     5281 catattcctg actaaaattt ttaatttctc ttacttttct tatttttttt ccaattaatt
     5341 tatccatcta attaattttt tataagttaa aagtaataaa atattaatat agttctttaa
     5401 tttttattgc attatttatt taatgttgca ttaaattaat attatattgt ttaatttaaa
     5461 taggtgtttt atgaaaaagt taatttattt ttttttatta gtatttattg gcactacatt
     5521 atcttgtaat aaagaaaata tagtgaaaga agtttcaacg aaagaaagaa ttttttccga
     5581 agaagagatg gtgtgggtaa ctcctaatgg atatgttata cctgctaccg aacgtaatca
     5641 ttgggaaaag tatatagctc aaacttttaa tgaaaaggca cctaaaggat atgaaagtag
     5701 ttcatgcact acagaaagta ttaaatgcgg tttaaagtgc tctgcgagtc gacttgataa
     5761 ttgttctaag gagatgtcgt gtacaccatg tatgaattgt ggttgtaccc caattaagag
     5821 cccttattaa ttttattata cttttcttta tctttagggt tgttttttta aaaataaccc
     5881 taattatatt agctgaattc aagtcacaaa tgcaacttct gagttaaaca ataatttttc
     5941 catta
//
LOCUS       DAOQVQ010000103         5905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14897_length_5905_cov_3.488205, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000103 DAOQVQ010000000
VERSION     DAOQVQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14897_length_5905_cov_3.488205"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2876)
                     /locus_tag="PKN22_04510"
     CDS             complement(<1..2876)
                     /locus_tag="PKN22_04510"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS41999.1"
                     /translation="MLFVSIQLNAQEVKHPHSIYYSFIENKGQWNDQVLFKSSFQGGN
                     MWIQQHKFVFHFQDYSATKNAHLGKSFVANNENKQQVVHFNFLNSNLVTSINKEGKTA
                     AYYNYFIGNNPSKWASDVRGYSEATLGNLYDGIDLKLIESEQQLKYEFHVRPQVDPNQ
                     IKIEIAGSNKVFIDKKGNLNVVTVAGQLLEEKPYAYQIKNGKIIQVACAFVLNGNQLS
                     FQIGNYDPTVQLVIDPTLVFATYSGSVTDNFGMTATYAHDGCAYSGGTIYGNSYPTPD
                     ANAYDVTSNFTVATGAGYGITDVFISKYSPDGTTMLWTTFLGGGDNTNGTETVHSLIA
                     DKNDNLYLFGATSSTDFPIVNGYQTTHGGGTPGSNFYYNGVYYSSNGSDIYVAKLSAN
                     GHNLMGSTYMGGSANDGLNYKLTSGNYSSYASYDSLTNNYGDQFRGEIMLDQFDNCLV
                     ASCTRSTNFPTLNAFQPALAGQQDGVVFKLNSNLSSLLWSSYYGGSNNDACYSVKIDS
                     SYNVVVGGGTSSNDIPGTAGGWQSAYNGGKGDGFVFKLTPNGQTITQATYVGTPNLDQ
                     VFFVEIDRNDKVFVLGQSAGGLFPVVNSGYSNAGSSQFIAKLNETLTGVENSTVFGNG
                     TPTINISPSAFMVDICGNVYTSGWGANILQPSGLSGMPVTADAFQSNPPNGFDFYLFV
                     LKRDFSSVLYASYIGGNQSNEHVDGGTSRFDKNGIVYQSVCGGCGGHSDFPTTPGAHS
                     NANLCTNCNNLVFKFDFNLMPDAEFTPDKIIGCAPFTVTLSNFSSASDSYLWDFGNGD
                     TTSVIFNPTITYSTPGSYVVELFVTDSICLMTDSAAITITVTDSLQLTTTPDQVLCVP
                     SPLTLTAFTNGTATNFTWSDNLSFTPVLNTNPADSNFVVTPNGPITYYVKVENPGCSK
                     IDSVRVDFIGSSVQLSGNDTLCLGQSTTLTAQSTNPLINFTYTWS"
     gene            complement(2968..4038)
                     /locus_tag="PKN22_04515"
     CDS             complement(2968..4038)
                     /locus_tag="PKN22_04515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNS42000.1"
                     /translation="MKLNIDEKEKQQLELGEEEELYEHHRVVVDPGTVMIRIDKYLMD
                     RLPNTSRNRIQNSAKSGFIIVNGNKVKQNYRVKPNDVISLVLPFPAQELDLIPEDIPV
                     EIPYEDDDLLIVNKPADMVVHPGLGNYRGTLVNALVHHIENLPHRTQDYYGRPGLVHR
                     LDKHTTGLLVVAKTEYALNHLAKQFFDRTVERRYWALVWGDVKEDGTVTGHIGRSLKN
                     RKVFMVYPEGDHGKHAVTHYKVLERFGLVTLVECKLETGRTHQIRVHMKHIGHTLFND
                     LEYGGDKILKGTTSNKYATFIQNCFDLIAGQALHAKTIGFMHPTKNEFMQFNSELPAG
                     YQQILEKWRNYTKNEDLELEED"
     gene            complement(4035..>4334)
                     /locus_tag="PKN22_04520"
     CDS             complement(4035..>4334)
                     /locus_tag="PKN22_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42001.1"
                     /translation="EGSVMIRPIFSTSLDGELGLPELVASTLAEVIIYPNPSSGEFNI
                     QSNIPVGEVYVYSLMGELVTTSTTNKFDLSNHPDGIYFVKTSTDFSKTYKVVKSK"
     assembly_gap    4335..4344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4345..>5905)
                     /locus_tag="PKN22_04525"
     CDS             complement(<4345..>5905)
                     /locus_tag="PKN22_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42002.1"
                     /translation="KSIQVGDLTNYPVQYQTSTGYPPYFIFDTIGIADTPDTVWIVGP
                     DLTQDSARVFFAHIVDPTKYWIDDYAYHNYRFAKDPWSIGVMTFDGIDNKGYPYQMGT
                     ALAGEADFLTSKPIDLSGNTPADSLYITFLYQKQGWGDAPESQDSLILEYYDATAQQW
                     RWQWSTNGGTVNEFNVAQVVIDNPAYFNDGFQFRFKNYGGLSGSLDHFHIDYVKVRAF
                     SGYQDTLIEDFAMSYPTVTLLKDYTSVPWDHYKNNPTNKTSIEVPLTVRNSYLNGGAN
                     ITSAGGGRIEVKYNGTSEGVVNLNGQIIANYNPPTQPIPDYQPRTTYHSNHDVSSFQF
                     DPTKTGNQQTFDIITSVSVPVGSNFLPNDTTYSTQYFGNYYSYDDGSAELAYGPQGAQ
                     SQLAIKYVPYEADSVIGAYIHFVPTVNDVTNKLFQLTIWADNGGKPGAVLYQDNSLNL
                     RQPSYGYGKNAFVTYLTQDTLKVPVSGTFYIGWKQIDAMRLGVGLDQNTDKHQNTYYS
                     VDNGATWLQSQI"
BASE COUNT         1830 a         1190 c         1008 g         1867 t
ORIGIN      
        1 gaccaagtat atgtaaaatt gattaatgga ttagtgcttt gtgctgtcag agttgtagat
       61 tgccccaaac acaaggtatc attgcccgac aattgcacac ttgaaccaat aaaatccact
      121 cttacactgt caatttttga acatccgggg ttttctactt taacataata ggtaatcgga
      181 ccgtttggag ttacaacaaa attggaatcc gctggattgg tatttaaaac tggtgtaaaa
      241 ctcaaattat cgctccatgt aaaattggta gccgttccgt ttgtaaaagc agttaaagtt
      301 aatggagatg gaacacataa aacttgatcg ggagttgtgg ttaattgcaa agaatcggta
      361 acagtgatag taatcgctgc gctgtctgtc atcaaacaaa tgctgtctgt aacaaacaat
      421 tctacaacat aacttcctgg tgttgagtag gtaatggttg gattaaaaat caccgaagtg
      481 gtatcgccat ttccaaaatc ccataaatac gagtcagaag ccgatgaaaa attactcaat
      541 gttactgtaa atggtgcgca accaataatt ttatcgggtg taaattcagc atcgggcatt
      601 agattaaaat caaacttaaa caccaaatta ttgcaattgg tacataaatt ggcatttgag
      661 tgagcccctg gtgttgtagg gaaatcacta tgacctccac aaccgccaca aactgattga
      721 taaacgattc catttttatc aaagcgagaa gttcctccgt ccacatgttc attagactga
      781 tttccaccaa tataacttgc atacaaaaca cttgaaaaat cgcgtttcaa tacaaataaa
      841 taaaagtcaa atccattggg tggattggat tgaaaagcat ctgctgtaac tggcattcca
      901 gacaaacccg aaggttgcaa aatattggct ccccacccac ttgtataaac gttaccgcaa
      961 atatccacca taaatgccga aggtgaaatg ttgattgtag gcgtaccatt cccaaaaact
     1021 gtcgagtttt caactcctgt caatgtttcg tttaattttg caataaactg tgaactacca
     1081 gcattggaat aaccagaatt tacaactgga aacagacctc ctgcactttg tcccaaaaca
     1141 aaaactttgt catttctatc tatttccaca aaaaatactt ggtctaagtt gggcgttccc
     1201 acatacgttg cttgtgttat tgtttgacca ttgggtgtta atttaaacac aaaaccatcg
     1261 cctttccctc cattgtaggc actttgccaa cctccagcag ttcccggaat gtcattagaa
     1321 ctagtaccac cgccaactac aacattatac gatgaatcga ttttaacgga ataacaagca
     1381 tcattatttg aaccgccata atacgacgac cacaacaaac tcgataaatt agaatttaat
     1441 ttaaaaacaa caccatcttg ctgtcctgcc aatgctggtt gaaatgcatt taatgttggg
     1501 aaatttgtag aacgcgtgca cgatgccact aaacaattat caaattgatc caacataatt
     1561 tctcctctaa attgatcgcc ataattattg gtcaaagaat cataagaggc ataggagcta
     1621 taattccctg aagtcaattt gtagttcaat ccatcgtttg ctgaacctcc catataggtt
     1681 gaacccatta aattatgacc atttgctgat aatttggcta cataaatatc cgacccgttt
     1741 gaagaataat aaactccgtt gtaataaaaa tttgaaccag gtgtgccccc cccatgcgtt
     1801 gtttgatatc catttacaat tggaaaatca gtgcttgaag tagcgccaaa caaatacaaa
     1861 ttatcgtttt tatcggcaat taaactatga acggtttctg ttccgttggt gttatcgccc
     1921 cctcccaaaa aagtagtcca caacatggtg gttccatcag gcgaatattt tgaaataaat
     1981 acatcggtaa ttccataacc tgcacctgtt gcaactgtaa aattggatgt aacatcataa
     2041 gcattggcat ctggtgttgg ataactattc ccataaattg ttcctccaga ataggcacat
     2101 ccatcgtgag cataagtggc cgtcatccca aaattatcag taacagaacc actgtaggtt
     2161 gcaaaaacca aggtaggatc aatcactaat tgcactgttg gatcgtaatt tccgatctga
     2221 aaacttagct gattgccatt caacacaaag gcacaagcaa cttgaataat ttttccattt
     2281 ttgatttgat aggcatacgg cttttcttct aataattgcc cagcaacagt aactacattc
     2341 agatttcctt ttttatcaat aaacaccttg ttactcccag caatttctat tttaatttga
     2401 ttgggatcaa cctgtgggcg tacgtgaaat tcatatttta attgttgttc agattcgatt
     2461 aatttcaaat caattccatc gtataaattt cctaacgttg cttctgaata acctctaaca
     2521 tcactagccc atttggaggg attgttgcca ataaaatagt tgtaataagc tgctgttttt
     2581 ccttctttgt ttattgaagt gacaaggtta gaattcaaaa aattaaaatg cacaacttgc
     2641 tgcttgtttt cattatttgc aacaaaagat tttcctaaat gagcattttt tgtggcgctg
     2701 taatcttgaa aatggaatac aaatttatgc tgttgtatcc acatattgcc cccttgaaaa
     2761 ctcgatttaa acaaaacttg atcgttccat tgccccttat tctcaataaa cgaataataa
     2821 attgaatgcg gatgcttcac ttcttgagcg tttagctgaa ttgaaacaaa caacattaaa
     2881 agaaaaatac aaattttcat ggagtagtac cttttttgct aatatactat ttttttataa
     2941 gacgaaaaaa aaatgcaaaa gttcctacta atcttcttct aactctaaat cttcgttttt
     3001 agtgtaattg cgccactttt ccaatatttg ctgatatcca gcaggcaact ccgagttgaa
     3061 ttgcatgaat tcatttttgg taggatgcat aaaccctatt gttttggcat gcagcgcttg
     3121 accagcaatt aaatcaaagc aattttgaat aaacgttgcg tatttattgc tagttgtacc
     3181 tttcaaaatt ttatcgcctc cgtattccaa atcgttgaat aaagtatgtc caatgtgttt
     3241 catgtgaaca cggatttggt gcgtgcgacc agtttctaac ttacattcta ccaacgttac
     3301 caagccaaaa cgttctaaaa ctttgtaatg cgtaacggca tgttttccat gatctccctc
     3361 aggatacacc ataaacactt ttcgattttt taaagaacga ccgatgtgac cagtaaccgt
     3421 tccatcttct tttacatcgc cccacactag tgcccagtac ctgcgttcta ccgttctgtc
     3481 aaaaaattgt tttgccaaat ggttaagtgc atattctgtt tttgcaacaa ccaacagtcc
     3541 agttgtatgt ttatccaatc ggtgcaccaa acctggtcga ccataataat cttgcgttcg
     3601 atggggtaaa ttttcgatgt gatgtaccaa tgcgttaacc aacgttccac ggtaatttcc
     3661 taatcccgga tgaacaacca tatcagctgg tttgttaacg atgagtaaat catcatcttc
     3721 gtaggggatt tcaactggaa tatcttctgg aatcaaatcc aattcctgag caggaaaagg
     3781 caaaaccaac gaaattacat cattgggttt aactctataa ttttgtttta ctttattgcc
     3841 attaacaatg ataaaaccgc ttttggctga attttgaata cgatttcgcg atgtattggg
     3901 caatctgtcc atcaaatatt tgtcaatccg aatcatcact gtgcccggat ccacaactac
     3961 acgatggtgc tcatataatt cttcctcttc gcctaactct aactgttgtt tttctttctc
     4021 gtcgatgttt aatttcattt tgacttcacc actttatatg ttttcgaaaa atcagtagaa
     4081 gttttcacaa agtaaatgcc atcaggatga ttggacaaat caaatttatt ggttgtagag
     4141 gttgttacta attctcccat caaagaataa acatacacct ctccaacagg aatattggat
     4201 tgaatattaa attctccgct tgatggattg ggataaataa taacctctgc taaagttgat
     4261 gctactaatt ctggcaatcc aagttctcca tccaacgagg ttgagaagat gggacgtatc
     4321 atcaccgaac cttcnnnnnn nnnncaattt gcgattgtaa ccaagttgca ccattatcca
     4381 cactataata ggtgttttga tgtttatcgg tgttttgatc caatcctaca cctaaacgca
     4441 ttgcatcaat ttgtttccaa ccaatgtaga atgttccaga cacaggcact tttaaagtat
     4501 cttgtgttaa ataggttaca aatgcatttt ttccataacc ataggatggt tggcgcaaat
     4561 tcaacgaatt atcttgatac aacacggctc ctggttttcc accgttatct gcccaaatag
     4621 tcaactgaaa caatttgttg gtaacatcat ttactgttgg tacaaaatga atgtaagcac
     4681 caattacaga gtcagcctcg tagggcacgt attttatggc caattgcgac tgcgcacctt
     4741 gtggtccata agccaattcg gcacttccat cgtcgtagga atagtaatta ccaaaatatt
     4801 gggttgaata agtcgtgtca tttggcaaaa agttagaacc tactggcaca gaaacactgg
     4861 ttatgatgtc aaatgtttgt tgatttcctg tttttgttgg gtcaaactga aaagaagaca
     4921 catcgtgatt ggaatgatag gtcgtgcgtg gttggtaatc tggaatgggt tgtgttggcg
     4981 gattgtagtt tgcaataatt tgtccgttta agttaacaac tccttcactc gttccgttgt
     5041 atttcacctc tattctacca cctccggcag atgtaatatt tgctcctccg ttcaaatagc
     5101 tgtttctaac tgttaacgga acttcgattg atgttttatt ggttggattg tttttataat
     5161 ggtcccaagg cacagatgtg taatctttta acaaagtaac agttggataa gacattgcaa
     5221 aatcttcaat caacgtatct tgataacccg aaaatgctct aactttcaca taatctatgt
     5281 gaaaatgatc aagagatccc gataatcctc cataattttt aaatcgaaac tgaaatccat
     5341 cgttgaaata agctggattg tcaatcacca cttgtgcaac gttaaattca ttgacagttc
     5401 ctccattggt actccactgc catctccatt gttgtgctgt tgcatcgtaa tattccaata
     5461 tcaatgaatc ttgtgattct ggagcatctc cccaaccttg cttttggtat aaaaatgtga
     5521 tatacaaact atctgcaggt gtatttccag ataaatcaat tggttttgaa gttaaaaaat
     5581 cagcttcacc tgccaatgca gttcccattt gatagggata gcctttgttg tcaatgccat
     5641 caaaagtcat taccccaatc gaccaagggt cttttgcaaa acggtagttg tgataggcat
     5701 aatcgtctat ccaatatttt gttggatcaa ctatatgagc aaagaaaaca cgagctgaat
     5761 cttgtgttaa atctggtccc acaatccaaa ctgtatctgg tgtatctgca attccaatag
     5821 tatcaaaaat aaaataaggc ggataaccag tagaagtctg atattgaaca gggtaattag
     5881 tcaaatcacc cacttgaatt gattt
//
LOCUS       DAOQVQ010000104         5891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14935_length_5891_cov_4.164496, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000104 DAOQVQ010000000
VERSION     DAOQVQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14935_length_5891_cov_4.164496"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKN22_04530"
     CDS             complement(<1..646)
                     /locus_tag="PKN22_04530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS42003.1"
                     /translation="MKDVSIVILNWNGRNYLKQFLPSVIKYSGDARIIVADNASTDDS
                     VEFLQQHFPQVEIIVNETNGGFAKGYNDALKKVDAKFYVLLNSDIEVTENWLQPLYQL
                     LQNDKIAGCQPKIRSYSEKHKFEHAGAVGGFIDANYFPFCRGRIFDSVEEDEGQYDGV
                     SEVFWATGAALMVRSDVYHQLGGLDESFFAHMEEIDWCWRAKKLGYALYVQPESV"
     gene            complement(660..2126)
                     /locus_tag="PKN22_04535"
     CDS             complement(660..2126)
                     /locus_tag="PKN22_04535"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNS42004.1"
                     /translation="MKNRSLLLICLFVFMSIGVLNAQGNKNKRLAAYLQVKQFYTPDA
                     GDYVELQFQYVGHSLNYVPKGKDLIGEIAVIIKINQGEKTVAQDAYRLNSPIMVDSIV
                     DDFYDIKHFALAPGNYQCQLELLDLNSKNPSVKSTFSFDVEEYADALSISDIIVAESA
                     TKTQQQNVFSKSGYDILPRIATFYPTELTNLPIYFEVYNSTQIEDSTFIIRQQVKNLE
                     TAQVLPEFTSFSKHQKAAVVPVFKSLNITNIPTGRYALSMTIFDKKSNELSTQTYEFE
                     RANDQDTVVNFADLILDPAFQQSITDDSVKFYLASLIPISGQGQARTILKEVRRKSND
                     KELQRKMIQAIWKQIDPVNTYEAWMRYKQQVLFVEENFKNNFQPGFETDRGRVYLQYG
                     APNRTIQREVSASELPYEIWEYNKIGKYSNKKFIFYNPDLVNNNYRLLHSDMIGELKN
                     PNWQYELNRRNSKHGSVDDGNEYVPDSWGNNSKQLFED"
     gene            complement(2244..2561)
                     /locus_tag="PKN22_04540"
     CDS             complement(2244..2561)
                     /locus_tag="PKN22_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNS42005.1"
                     /translation="MEDHNNDNQINIELTDDVASGVYSNLAIITHSPGEFVTDFIQLM
                     PGLPKGKVKSRIIMTPQNAKRLLGALADNIQKYEQAFGVIEEIEPNNGVARMNFGTPT
                     TKA"
     gene            2688..3749
                     /gene="rfbB"
                     /locus_tag="PKN22_04545"
     CDS             2688..3749
                     /gene="rfbB"
                     /locus_tag="PKN22_04545"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNS42006.1"
                     /translation="MKKTFQKNILITGGAGFIGSHVVRLFVNKYPDYRIVNFDKLTYA
                     GNLSNLKDVENKENYVFFKGDIVSPDDVKLVFDLYQISDVIHLAAESHVDRSIEGPMD
                     FVQTNVVGTVNLLQTAKNYWTDFSNHVFHHVSTDEVYGSLGEDGFFEETTAYDPRSPY
                     SASKAASDHFVSAFYHTYGLPVKLSNCSNNYGSHHFPEKLIPLMINNIVNKKPLPVYG
                     KGENVRDWLWVEDHARAIDVIFHTGKIGESYNVGGLNEWRNIDLVNYLCTILDKKLNR
                     APGESAQLITYVKDRAGHDLRYAIDATKLATELGWKPSITFEEGLEKTVDWYLHNQEW
                     INNITSGDYKYYYEKMYNN"
     gene            3757..4527
                     /locus_tag="PKN22_04550"
     CDS             3757..4527
                     /locus_tag="PKN22_04550"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HNS42007.1"
                     /translation="MGLLSIFKPQQILPAIDFSQLVVDMHSHLIPGIDDGAKNMDETI
                     AMLAKFESLGYKKVITTPHVYSDCYRNTPETILGGLKNVQKTAKDLGLTIEIEAAAEY
                     YCDEYFLDLIEKKELLPIDNKYVLMEFSFHAEMDNWREFVFGVRAAGYTPIIAHFERY
                     IYWLGSTKVAQEMVELGAKLQLNYNSLAGHYGPSIKKQAELLISNNLISFLATDCHRI
                     QHLRIMEDNANNAFVHKLLNSGLLLNNQLMKQEHALVH"
     gene            complement(4538..5113)
                     /locus_tag="PKN22_04555"
     CDS             complement(4538..5113)
                     /locus_tag="PKN22_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HNS42008.1"
                     /translation="MLLNQLQERANHQCELCASTNDLSVFLVAPKTDETLENTILACS
                     TCVEQINDESKMDVNHWRCLNDSMWNENAAVKVVVWRMLDRLRAEGWPVDLLDMMYME
                     EADLKWAKESAQAPTETAVVHKDSNGVVLEAGDTVVLIKDLPVKGSSLVAKRGTAVRR
                     ITLDRDNSNYIEGRVEGQQIVILTQYVKKSS"
     gene            5327..>5891
                     /locus_tag="PKN22_04560"
     CDS             5327..>5891
                     /locus_tag="PKN22_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNS42009.1"
                     /translation="MINITLPDGSVRQYESGVSPMDIALSISEGLARNVLAAKINDKV
                     VDANTAITTDVALQLLTWNDTDGKSTMWHSSAHLMAEALEFYYPGIKLAIGPPVANGF
                     YYDVDFMDYTIQEKDLEKIEQKMKELAKLKSPFVRKEVSKADAIAYFTEKQDPYKLEL
                     IEGLEDGNITFYTQGNFTDLDIYLKKRR"
BASE COUNT         1868 a         1118 c         1011 g         1894 t
ORIGIN      
        1 ccaccgattc tggttgcaca tataatgcat atcccaactt tttggcgcgc caacaccaat
       61 ctatttcttc catgtgggca aaaaacgatt catccaaacc gcccaattga tggtaaacat
      121 cagagcgcac catcaatgca gctcctgttg cccaaaaaac ttccgaaaca ccatcgtatt
      181 gtccttcatc ttcttcaaca ctgtcaaaaa ttcgacctct acaaaaagga aaatagttag
      241 catcaatgaa acctccaacg gctccagcgt gttcaaattt atgtttttcg gaataacttc
      301 taattttggg ttgacaacca gcaattttat cgttttgtag gagttgatac aacggttgca
      361 accaattttc agtaacttcg atatcggaat tgagcagcac ataaaactta gcatccacct
      421 tttttagcgc atcgttgtag ccttttgcaa atccgccatt ggtttcattc actatgattt
      481 caacttgtgg aaaatgctgt tgtaaaaatt caactgaatc atcggtagaa gcattgtcgg
      541 caacaattat tcgtgcatcg ccagaatatt taatcactga gggtaaaaac tgttttaagt
      601 agttacgccc attccagttt aaaataacaa tacttacgtc tttcacttat gacaatcaat
      661 taatcttcaa acaattgctt agaattattt ccccaagaat ctggcacgta ttcatttcca
      721 tcatccacac ttccgtgttt agaatttcta cggttcaact cgtattgcca attcggattt
      781 ttcaattcac ctatcatgtc agagtgcaac aaacggtaat tgttgttaac taaatctggg
      841 ttgtagaaaa tgaatttttt attactgtat ttgccaattt tgttgtattc ccaaatctcg
      901 tatggcaatt cagaggctga aacttcgcgt tggatggtac gattaggcgc tccatattgt
      961 aggtaaacac gtcctctatc tgtttcaaat ccaggttgga aattgttttt aaagttttct
     1021 tcaacaaaaa gcacttgttg tttatagcgc atccatgctt catacgtgtt aacaggatct
     1081 atttgtttcc aaattgcttg aatcattttt cgttgcaatt ctttatcgtt gctttttcta
     1141 cgtacttctt ttaaaatcgt tcttgcttgt ccttgccctg aaatcggaat caaactcgcc
     1201 aagtaaaatt taacgctatc atcggtgatg gattgttgaa atgctggatc taaaattaaa
     1261 tctgcaaaat taactaccgt atcttgatcg ttggcacgtt caaattcata cgtttgagtg
     1321 gataactcgt tggatttttt atcaaaaatc gtcatactca acgcatatcg acctgttgga
     1381 atattggtga tattcaatga tttaaaaaca ggaacaactg ctgctttttg gtgttttgaa
     1441 aacgaggtga attcgggtaa aacttgtgct gtttctaaat tctttacttg ctgacgaatg
     1501 ataaacgtag aatcctctat ttgtgtagaa ttatacactt caaaataaat aggaagattg
     1561 gttaattcgg ttggatagaa cgttgctatt cgcggcaaaa tatcgtatcc agatttagaa
     1621 aacacatttt gttgttgcgt tttggttgcc gattcagcca cgataatatc cgaaatagac
     1681 aaagcatctg catattcctc tacatcaaaa ctaaaagttg attttacaga aggattttta
     1741 gagtttaaat ccaacaattc caactgacat tggtaatttc ctggtgctaa tgcaaaatgt
     1801 ttgatgtcgt aaaaatcatc aacaatacta tccaccataa tgggcgagtt taatcgatag
     1861 gcatcttgcg ccaccgtttt ctcaccttgg ttgattttaa tgattacagc tatttctcca
     1921 attaaatcct ttcctttagg cacataattt aacgaatgac caacgtattg aaattgaagt
     1981 tccacataat cacctgcatc tggcgtgtaa aattgcttca cttgcaagta agccgccaac
     2041 cgtttgtttt tatttccttg agcatttagc acgccaattg acataaaaac aaataagcaa
     2101 atcaatagta aacttctatt tttcatatta aaattcattg aattattgag gtaaagataa
     2161 caacttatgt tcacctttcc atcaactgta tcagctattt agtaatccgt aaaaaattgc
     2221 gttttaatgg ttatccaaac aaattatgcc tttgttgtgg gtgtaccaaa attcatgcgt
     2281 gccacaccat tgtttggttc aatttcttca ataactccaa atgcttgctc gtatttttga
     2341 atattatcgg ccaatgcacc caataaacgt ttagcatttt gaggcgtcat aatgatgcgc
     2401 gattttactt taccttttgg caatcctggc attaattgaa taaaatctgt cacaaattct
     2461 cctggagaat gggtaatgat tgcaagattg gaatatacgc cagaagcaac atcatctgtt
     2521 aattcgatat tgatttgatt gtcattgtta tgatcttcca ttggattttt attttaacgt
     2581 taatgaaagt gctaaaataa tatttttatc gatattttag aagaattttg attgcctatt
     2641 caataaaaaa aactatctta gcgtttcatt actgaaacat aactaaattg aaaaagacct
     2701 ttcaaaaaaa catattaatt accggaggag ctggttttat tggttctcac gtagttagat
     2761 tatttgtcaa taaatatcca gattatcgaa ttgttaactt cgacaagttg acctatgctg
     2821 gtaatttaag taatttgaaa gatgttgaaa acaaagaaaa ttatgttttt ttcaaaggcg
     2881 acattgtttc acctgacgat gtgaaattgg tgtttgatct ttatcaaatt tcagacgtta
     2941 ttcaccttgc agcagaatca catgttgacc gttcaattga aggtccgatg gattttgtgc
     3001 aaaccaacgt tgtgggaact gttaatttgc ttcaaacagc aaaaaattat tggacagatt
     3061 tttccaacca cgtttttcac catgtttcaa ccgacgaggt atatggttcg ttgggcgagg
     3121 acggtttttt tgaggaaaca acggcatacg accctcgcag tccgtattca gcatccaaag
     3181 cagcatccga tcattttgtg agtgcgtttt accacaccta cggattgccg gtaaaacttt
     3241 ccaattgttc taataactac ggaagtcatc atttccctga aaaattaatt cccttgatga
     3301 ttaacaacat cgttaacaaa aaaccattgc ctgtttatgg aaaaggtgaa aatgttcgcg
     3361 attggttgtg ggtagaagac catgcacgtg ccattgatgt tatctttcat actggaaaaa
     3421 ttggcgaatc ctataatgtg ggtggactca acgaatggcg taatattgac ttggtcaact
     3481 atttatgcac aatcttggat aaaaaattaa accgtgctcc aggtgagtcg gcacaattga
     3541 ttacttacgt aaaagacaga gctggacacg atttacgcta tgcaatagac gctacaaagt
     3601 tagccactga attggggtgg aaaccctcca ttacttttga agaaggatta gaaaaaacag
     3661 ttgattggta cctccataac caagagtgga tcaacaacat aacttctgga gattataaat
     3721 actattacga aaaaatgtac aataactgaa aaaaacatgg gactactcag cattttcaaa
     3781 ccacaacaaa tactaccagc aattgatttt agtcaattag ttgtagacat gcacagccac
     3841 ttgattcccg gcattgacga tggtgccaaa aacatggatg aaaccatagc catgttggct
     3901 aaatttgaat cattgggcta taaaaaagta attactactc cgcacgttta cagtgattgt
     3961 tatagaaata caccagaaac tatactagga ggattaaaaa acgttcaaaa aacagccaaa
     4021 gatttgggac taaccattga aattgaagct gctgcagaat attactgtga tgaatatttt
     4081 ttagacctca tcgagaaaaa agaattgtta cctattgata acaaatacgt tttgatggaa
     4141 ttttctttcc atgctgaaat ggataattgg cgtgaatttg tgtttggagt acgcgctgca
     4201 ggctatactc ccatcattgc tcattttgaa cgttacattt attggcttgg ctcaacaaaa
     4261 gttgcacaag aaatggtgga attgggagca aaactacagt tgaactataa ttcattagca
     4321 gggcattatg gtccttctat taaaaaacaa gcagaactat tgatttccaa caatcttatt
     4381 agttttttag ctacggattg tcaccgcatc caacacttgc gcatcatgga agacaatgct
     4441 aacaatgcgt ttgtacacaa attactcaat agcggattat tgctcaacaa tcaattgatg
     4501 aagcaagaac acgcattagt gcactaaccg tatattttta cgatgatttt ttaacgtatt
     4561 gtgtaagaat aacaatttgt tgtccttcca ctctaccttc aatgtaattg gaattatctc
     4621 tatcgagcgt aattcttcgc acggctgttc ctctttttgc aaccaagcta gagcctttta
     4681 caggcaaatc tttgattaac acaactgtgt cacccgcttc tagcacaaca ccgttcgaat
     4741 ctttatgtac aaccgctgtt tctgttggtg cttgagcact ttcttttgcc cattttaaat
     4801 cagcttcttc catgtacatc atgtccaaca aatcaacagg ccaaccttca gctcttaaac
     4861 gatccaacat gcgccaaacc accactttta cagcagcatt ttcattccac atgctatcgt
     4921 ttaaacaacg ccagtgattc acatccatct tactctcatc gttgatttgc tctacacaag
     4981 ttgaacaagc aagaatggta ttttctagtg tttcatctgt ttttggtgct actaaaaata
     5041 cacttaaatc gttggttgaa gcacacaatt cacactggtg attggctctt tcttgaagtt
     5101 ggtttaataa catgttctac taaattttgg gacaaagata gcagaatttt ctaaaggtta
     5161 agatgggatg tttgctatga ataaaaatcc acttgatcca aaaccatcca caatttcaat
     5221 aaaaacttaa ccataaacac tgcaaataat tctatgatat tacaataatt caattacata
     5281 ttcttatctt tgcaatctta attttaaccg tttatagaaa aacacaatga taaacattac
     5341 acttccagac ggatcagtca gacagtatga aagcggtgtt tctccaatgg atattgcttt
     5401 gagcatttct gaggggttag caagaaacgt gcttgccgct aaaattaacg ataaagtggt
     5461 ggatgccaat acggctatta ccactgatgt tgctttgcaa ttactgacgt ggaatgatac
     5521 cgatggaaaa tcaaccatgt ggcactcttc ggctcactta atggctgagg cattggagtt
     5581 ttattatccg ggaattaaat tagcaattgg accgcctgtt gccaacggtt tttactacga
     5641 tgttgatttc atggattaca ccatccaaga aaaagattta gaaaaaatcg aacaaaaaat
     5701 gaaggaattg gcaaaattga aaagtccttt cgtacgaaaa gaagtttcaa aagcagacgc
     5761 aattgcctat tttacagaaa aacaagatcc ttacaaatta gaattaattg agggattaga
     5821 ggatggtaac atcacttttt atacgcaagg aaactttact gatttggaca tttacctgaa
     5881 aaaacgacgc t
//
LOCUS       DAOQVQ010000105         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_14955_length_5886_cov_3.441948, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000105 DAOQVQ010000000
VERSION     DAOQVQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_5886_cov_3.441948"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKN22_04565"
     CDS             <1..502
                     /locus_tag="PKN22_04565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS42010.1"
                     /translation="FLLIFSACTEEKKNDDFKWTTEESTKMNKHFSVQEDLDINLYLS
                     RKPEWKMTKTGTGLRYYIYENGSGETAQVDDVVDVEYTITLFDGTQCYATASDEVDEF
                     KVDKAQIESGIQEAVKLMKEGDKAQLIVPSHLAYGIIGDMSKIPPLSPLVVDIKLVKI
                     YRKTAP"
     gene            499..1332
                     /locus_tag="PKN22_04570"
     CDS             499..1332
                     /locus_tag="PKN22_04570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS42011.1"
                     /translation="MKQLIFIALCIFTLVACKPEGVKKEVLETSKKQSSKVDSLELKL
                     EVIDEEKLDNGLEIKWIEKSNGESIKAGDVILIDYKVRLADSTIIDGNHLLNKPYLPY
                     MVGFGFQPAGWDLAFEKMRVGDFARIKIPAKLARGDKEVKNLIPKNSDNYLTIRILSK
                     KAPTKEIDGVKIWLLEENKLQKARFNDKNSIEFFTTASSPSHPFYFNSYATNQPFRLK
                     LADHGIVPGLKKALINAKKGDRMYIYVPAKEAYDTKGYLDLVLPNEPIFYNLLVTDIL
                     D"
     gene            1394..2368
                     /locus_tag="PKN22_04575"
     CDS             1394..2368
                     /locus_tag="PKN22_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNS42012.1"
                     /translation="MFKKFNLFLLFISLSTILFSQSVIDTLYTSHGTVILYENKSWQY
                     LEDLRFDGVMNEHLNCVLKDHPDLDLKQHWDNNVVFPSGYSNSMANFNDTIWMCVLRD
                     INDEFVVPVPGALTSEYKFRGRRHHRGVDLDLETGDTVVAAWSGKVRYAKYNEGGFGN
                     LVIIRHDNGLETLYAHNSKLLVKPNQNVKAGTPIALGGNTGHSYGSHLHFEVRFFDLP
                     MNPEEIIDFQNKTCRSENLLIHRSLFIPSAKPSFETTPTTTSPTMNVAAPLAVKASTS
                     TNKYYKIRSGDTLSSIAAKHHTTISKLCQLNGIRQTTVLQVGRNLRIR"
     gene            2379..3197
                     /gene="bamD"
                     /locus_tag="PKN22_04580"
     CDS             2379..3197
                     /gene="bamD"
                     /locus_tag="PKN22_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNS42013.1"
                     /translation="MNLKNSIFYLFILQFLVVGCSDYNKVLKSEDYARKFELANSYFD
                     AEKYAQAMALYEQVYQRFPKQSEGEVAYFRLGKGYYLSKDYYMGGYFLGQFVSRFPFS
                     TKVEEATFLSAMCSVNQSPNKSLDQTETQVAINNLQQFVDRFPNSELVDSCNRIMDRL
                     QFKIESKSFDQVKQYSKTLNYRAAVTSAEIFLTNYPTSSYKEECEYILVNNSYLLTKN
                     SIDSKKLERTEQTLERYRNFATNFPSSKYLKSLNTIKVEMEKLLVKLENLEAEK"
     gene            3201..3524
                     /locus_tag="PKN22_04585"
     CDS             3201..3524
                     /locus_tag="PKN22_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNS42014.1"
                     /translation="MSFKNSTAEKTTITRDVINIEEKVGNVYEAIVVLAKRSNQLNVD
                     LKEELTQKLQEFASSTDNLEEIFENREQIEISRFYEKLPKPVAMAIEDLLKDKVYIRK
                     DEVIG"
     gene            3527..4723
                     /gene="coaBC"
                     /locus_tag="PKN22_04590"
     CDS             3527..4723
                     /gene="coaBC"
                     /locus_tag="PKN22_04590"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNS42015.1"
                     /translation="MHNKRILLGITGGIAAYKIAFLIRLLKNEGAEVKCIMTPASCDF
                     ISPLVVATLSGNPVGIEFWNKADGTWNNHVEYGLWADVFVIAPATANTLAKMATGACD
                     NLLLATYLSNKSKTIIAPAMDLDMYAHPTTSRNLEQLQKDGVKIIPAESGFLASGLEG
                     QGRMAEPETILNCIKAEVCTTNELEGKHILVTAGPTYEAIDPVRFIGNHSTGKMGYEI
                     AEALALKGAMVHLVSGPSTLSVKHPAITKTAVTSAQEMLAFVQENWNKMDGGIFSAAV
                     ADYRPKTVAQEKIKKKEGELTLELVKNPDILLWAGEHKEKHQLLVGFALETTNSVAYG
                     TEKLNKKNLDFIVINEPVEGKTGFGAPTNQVILLDNHNNLTKFELKSKSQVANDIVNY
                     YIKLLK"
     gene            4720..5631
                     /locus_tag="PKN22_04595"
     CDS             4720..5631
                     /locus_tag="PKN22_04595"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNS42016.1"
                     /translation="MKQLIVLISFCLTTFASLFAQELNCEVSIITSNKIEVSSLDHEV
                     LKQLEDVIRQFMNETKWTKDEFKMEERINCQLQFQVEKIPQPGVYEGKFQIQSSRPVY
                     NSTYNSVVLNFADQDVSFAFSRNAMLYYSSAQFKDNLTSMLAFYAYYIIGMDYDSFSP
                     KGGTQYLQEAQNIVVNAQSSGAPGWSPDQTGTKKNRYWIIDNALHQLFEPMRECTYEY
                     YRLGLDKLYENKEQGRNAMYEALNKLPKVVAARPNTININSFIYSKRTELKQIFADAP
                     TPEKGKLVALLKRLDPTNADKYDEILK"
     gene            5631..>5886
                     /locus_tag="PKN22_04600"
     CDS             5631..>5886
                     /locus_tag="PKN22_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS42017.1"
                     /translation="MLQQLNVQNFALIQQASLNFGAGYTVITGETGSGKSILLHALQL
                     ILGERADFKVIGASGDKAIVEGVFSINSDKFSEFFSTNDID"
BASE COUNT         2087 a          985 c         1091 g         1723 t
ORIGIN      
        1 gtttctgttg attttttcgg cttgtacgga agaaaaaaag aacgatgatt tcaaatggac
       61 cacagaagaa tcgaccaaaa tgaacaaaca tttttctgtc caagaagatt tagatatcaa
      121 cttgtattta tcgcgcaaac cagaatggaa aatgacaaaa acaggcaccg gtttgcgtta
      181 ttacatctac gaaaacggaa gtggcgaaac agctcaagtg gatgatgttg ttgacgtgga
      241 atacaccatt acactttttg atggtaccca atgttatgca actgctagcg atgaagtgga
      301 tgaatttaaa gttgacaaag cacaaataga atcaggtatt caagaagctg taaaacttat
      361 gaaagaaggc gacaaagctc aactaattgt tccaagccat ttagcttatg gaattattgg
      421 cgatatgagc aaaataccac ctttaagccc attagttgta gatattaaac tggttaaaat
      481 ttatagaaaa actgcaccat gaaacaattg attttcatcg cattatgtat attcactttg
      541 gttgcctgca agcccgaagg agtaaaaaaa gaagtgcttg aaacaagcaa aaagcaatca
      601 tcaaaagttg attctttgga gttgaaacta gaagttattg acgaagaaaa gctagataat
      661 ggattggaaa ttaaatggat agaaaaatcc aatggcgaat caataaaagc tggagatgtg
      721 atactaattg actacaaagt tcgtttagct gacagtacca tcatcgacgg aaaccattta
      781 ctcaataagc cttatttgcc ttacatggtt ggttttggat ttcagcctgc aggttgggat
      841 ttagcttttg aaaagatgcg agttggagat tttgcacgca tcaaaattcc agcaaaattg
      901 gctcgtggag ataaagaagt taagaactta atccctaaaa attctgataa ttatttaacc
      961 attcgaattt tatctaaaaa agcacctacc aaagaaattg atggagtcaa aatttggtta
     1021 ttggaagaaa acaaacttca aaaagcacgt tttaatgaca aaaacagcat cgagtttttt
     1081 acaacagcta gttctcctag ccatccattt tattttaatt cttatgctac aaatcagcca
     1141 tttaggttaa aattagccga tcatggaatt gttcctgggt taaaaaaagc gttaattaat
     1201 gctaaaaaag gagaccgcat gtatatatac gttccagcca aagaagctta cgacactaag
     1261 ggttacttgg atttggtttt accaaacgaa cctatttttt ataacctttt ggttaccgac
     1321 attcttgatt agttgaataa aaaacaatat atttgttcgt tttatactca aaatcaaaaa
     1381 aaatagaaaa gtaatgttta aaaaattcaa cttattttta ctgttcatct ccttatctac
     1441 catcctattt tctcaatcag taatagatac actttatacc agtcacggta ccgtgatttt
     1501 gtacgaaaac aaatcatggc aatatttaga agatttaaga tttgacggtg ttatgaacga
     1561 acacttgaat tgtgtattga aagaccatcc agatttagat ttaaaacaac attgggacaa
     1621 caacgtagta tttccatcag gatattcaaa ttcaatggcc aattttaacg atacaatttg
     1681 gatgtgtgtg ttaagagata tcaacgatga atttgtggtg ccagttccag gagcattaac
     1741 ttcagaatac aaatttagag gaagaagaca tcaccgtggt gtggatttgg acttagaaac
     1801 tggtgacaca gttgttgctg cttggtcggg taaagtgcgt tatgctaaat acaacgaagg
     1861 tggttttgga aatcttgtta ttattcgtca cgacaacgga ttggaaacat tgtatgcaca
     1921 caactctaaa cttttggtta aacccaatca aaatgtaaaa gcaggaacgc ctatagcttt
     1981 gggcggaaac acgggacatt cttacggttc acacttgcat tttgaagtac gcttctttga
     2041 tttacctatg aatccagaag aaattattga ctttcaaaac aaaacttgta gaagtgaaaa
     2101 cttattaata cacagaagtt tatttattcc aagtgcaaaa cctagttttg aaacaactcc
     2161 aacgacaaca tcaccaacta tgaatgttgc agcaccactt gcagttaagg cttcaactag
     2221 caccaataaa tattacaaaa tccgctcagg cgatacctta agctctatcg ctgcaaaaca
     2281 ccatacaact atttcaaaat tgtgtcaatt aaatggcatt cgacaaacaa cagttttgca
     2341 agtaggacgt aatttaagaa tccgttaata accattcaat gaatttaaaa aacagcatat
     2401 tttatctttt cattttacaa tttttagttg taggctgttc tgattacaac aaagtactaa
     2461 aatcagagga ttacgcacgc aagtttgaat tagcaaacag ctattttgac gcagaaaaat
     2521 atgctcaagc aatggcgctt tacgaacaag tttaccaacg tttcccaaaa caaagtgaag
     2581 gtgaagttgc ttatttcaga ttaggaaaag gatactattt aagcaaagat tactacatgg
     2641 gaggatattt tttaggtcaa tttgtatccc gtttcccatt tagtacaaaa gttgaagaag
     2701 ctacgttttt atctgcaatg tgttcggtta atcaatcacc aaacaaatca ttggaccaaa
     2761 cagaaacaca agttgctatc aataatttgc aacaatttgt tgaccgtttt ccaaactcag
     2821 aattggtaga ttcgtgcaat cgcattatgg accgcttaca atttaaaatt gaatccaaaa
     2881 gttttgacca agtaaagcaa tacagtaaaa cattgaacta tagagctgcc gtaacgtctg
     2941 ctgaaatatt tttaaccaat tatcctacca gttcctataa agaagaatgt gaatacattt
     3001 tagtaaataa ctcctatttg cttacaaaaa atagtataga ttctaaaaaa ttagaaagaa
     3061 ctgaacaaac tttggaaaga tatcgtaact ttgcaactaa tttcccttca tcgaagtatt
     3121 taaaaagttt gaataccatt aaagttgaaa tggaaaaact attagtaaaa ttagaaaatt
     3181 tagaagccga aaaataaatt atgagtttta aaaacagcac agcagaaaag acaacaatta
     3241 caagagacgt aatcaacatt gaagaaaaag ttggtaatgt ttatgaagca atcgttgttt
     3301 tggcaaaaag atcaaatcag ttgaatgttg atttaaaaga agaattgact caaaaattgc
     3361 aagagtttgc ttcatcaact gataacttgg aagaaatttt tgaaaaccgt gagcaaattg
     3421 aaatttcacg tttttacgaa aaattaccaa aaccagttgc catggcaatt gaagatttat
     3481 tgaaagacaa agtttatatt cgcaaagacg aagtaatcgg ataataatgc acaacaagcg
     3541 catacttctg ggtatcacag gcggtattgc agcatacaag attgcattcc tgatacggtt
     3601 attaaaaaat gaaggggcag aagtcaaatg tattatgact cctgcctctt gtgattttat
     3661 tagtccgctt gttgttgcca cactttctgg aaaccctgtt ggaattgagt tttggaacaa
     3721 agctgatggc acctggaaca accacgttga atatggttta tgggccgatg tatttgttat
     3781 tgctccagcc actgccaata cacttgcaaa aatggcaaca ggtgcttgcg ataatttatt
     3841 gttggcaacc tatttatcca acaaatcaaa aacaattatt gcccctgcaa tggatttgga
     3901 catgtatgcg catccaacca ccagcagaaa tctagagcaa cttcaaaagg atggggttaa
     3961 aatcatacct gccgaatctg gattcttggc aagtggattg gagggacaag gacgcatggc
     4021 cgaacccgaa accatactca attgcatcaa agctgaagtt tgtaccacta acgaattgga
     4081 aggtaaacat attttggtaa ctgccggacc aacttatgaa gcaatagatc ctgttcggtt
     4141 cattggcaac cattctactg gaaaaatggg gtatgaaatt gctgaagcat tggccttaaa
     4201 aggagctatg gtacatttgg tgagtggtcc ttctactcta tctgtaaaac atcccgcaat
     4261 aaccaaaaca gcagttacat cggcgcaaga aatgttggct tttgtgcaag aaaattggaa
     4321 taaaatggac ggtggtattt tcagtgcagc tgtggctgat taccgaccaa aaactgtggc
     4381 gcaggaaaaa atcaagaaaa aggaaggcga actaacgctt gaattggtta aaaatccaga
     4441 tattttatta tgggctggtg aacacaaaga aaaacatcaa ttgttagttg gttttgcatt
     4501 ggaaactacc aacagtgttg cttacggtac cgaaaaatta aataagaaaa atttagactt
     4561 tattgttatc aacgaacctg ttgaagggaa aacaggattt ggcgcaccaa caaatcaagt
     4621 tattttgttg gataatcaca ataatttaac gaaatttgag ttaaaatcga agtcgcaagt
     4681 ggctaatgac attgtaaatt attacattaa actattaaaa tgaaacaatt aattgttctt
     4741 atctcttttt gcttaaccac atttgcttca ctcttcgcgc aagagttgaa ttgtgaggtt
     4801 agcatcatta caagtaataa aattgaagtt agctcattgg accatgaggt tttgaagcaa
     4861 ttggaagatg taatccgcca atttatgaac gaaactaaat ggaccaagga tgaattcaaa
     4921 atggaagaac gcatcaattg tcagttgcaa tttcaagtag aaaagattcc gcagccaggt
     4981 gtgtatgaag gaaaatttca aatacaaagt tctcgtccag tttacaattc cacttacaat
     5041 tctgttgttt tgaattttgc tgatcaagat gtttcatttg cattttcaag aaacgcgatg
     5101 ttgtattatt cttcagctca attcaaagat aatttaactt caatgttggc attttatgct
     5161 tattatatta ttggaatgga ctacgattct tttagcccaa aaggtggaac tcaatattta
     5221 caagaagctc aaaatattgt tgtaaatgca caaagctctg gcgctccggg gtggagtccc
     5281 gaccaaacag gtaccaaaaa gaaccgctat tggattattg acaacgcttt gcatcagttg
     5341 tttgaaccaa tgcgcgaatg tacgtatgaa tattatcgtt taggattgga taaattgtat
     5401 gagaacaaag agcaaggaag aaacgccatg tatgaagcgt tgaacaaatt gccaaaagta
     5461 gtagcagcac gccccaatac catcaatatc aactcgttta tttattccaa acgtacagaa
     5521 ttaaaacaaa ttttcgcgga tgccccaaca cccgaaaaag gaaaattagt ggctttattg
     5581 aaacgtttgg accctacaaa tgctgataag tacgacgaaa ttttaaaata atgctccaac
     5641 aactgaatgt ccaaaacttt gcactcattc agcaagcatc tttaaatttt ggtgcgggct
     5701 atacggttat tactggcgaa acaggttctg gaaaatccat tttattgcat gcattgcagc
     5761 taattttagg tgaacgtgcc gattttaaag tcataggagc aagtggtgat aaagcaatag
     5821 tggagggagt ttttagcatt aattccgata aattttctga attttttagc accaacgaca
     5881 ttgatt
//
LOCUS       DAOQVQ010000106         5835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15121_length_5835_cov_3.702595, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000106 DAOQVQ010000000
VERSION     DAOQVQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15121_length_5835_cov_3.702595"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2054
                     /locus_tag="PKN22_04605"
     CDS             <1..2054
                     /locus_tag="PKN22_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42018.1"
                     /translation="DVKCEEAYLTYIPRKKILKASSMPRSEFEFFNKEANLKGTTIVT
                     PNGMEGNGLMTFKTATIVSTKFKYEHKDIFADTSSFNLRNQSREEGEAPLIFETNNVK
                     SHVSFKERKGEFNSNKGESRVDFPVNQYMCKMDKFTWFMDQEELTMEKQATKDVAINA
                     GVDLLGPNFFSTHPKQDSLQFRAPKAKLDMKAKTIYCNEVEYIDVADARIYPDSMKVN
                     IRKKAKLDQFTNATIIANYITKYHKFTKAVVDITARRAYTGSGEYPYYDRDSILSNVV
                     MNSITLDSTFQTVANGKIEQNAGFKLSPEFDYYGNITVKAASPEILFNGATRINHNCS
                     NFEKNWLAFTSNVDPKNVQIPVSSTMTNLENQAISAGIVWRDAANVDSIALYPTFLSK
                     LVSPTDPIVATSSGFLMYDNSTKEYQIASKEKLINRSAKGNFLSMNTETCALFAEGII
                     NLGMDHGDVTVSTAGMVDYNSNTGEALFNLTAKFDIPIDKSILKNIPEKINLTEGLKP
                     MDFTTNTLKSSITAWGTQAEADKLQENYTLKGEVKNIPNCLESTFTITGIRLNYYSQP
                     KLNNFKGLITTSESAILVNMFDKPVMKYIPFKAFFQQKYSGAGGDWFAFLIDIPGGKD
                     YFFNYSMGKKEGMMDIMTGDTELSTEIAALKEDKRKKKNFTYQIGRGGLKTVFYNLFG
                     N"
     gene            2054..2674
                     /gene="plsY"
                     /locus_tag="PKN22_04610"
     CDS             2054..2674
                     /gene="plsY"
                     /locus_tag="PKN22_04610"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNS42019.1"
                     /translation="MEFIYFGLIAYLIGSIPTAIWVGKIKYGVDVREHGSKNAGATNT
                     FRVLGKKAGIFVLTVDILKGFIASFLPYLFIDENLNPDKLALVQITCSFLAVLGHVFP
                     IYAGFKGGKGVATSLGVIIGVLPIAALICIIVFLIVFIASKYVSLGAICASATFPIVT
                     IFVFQSNSIWLKAFSIVLAAVVIIAHRKNIKRLLDGNESKMNLFKK"
     gene            2803..>5835
                     /locus_tag="PKN22_04615"
     CDS             2803..>5835
                     /locus_tag="PKN22_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42020.1"
                     /translation="MKAKADAFFVNNQFVEATPLYLRLLSLQPRDYEYSYKYGACLLY
                     NSNNKQDALRYLTYAVKNPVNTAEPHYFLGKAYHLNYQFNEAITEYKSYLQLAGTKAT
                     YSKDAQRNIEMCANGKQLISTITDIVVKKKTEINQNDFFRLYDLSDIGGTLITATNFQ
                     TKNDKKFNHTPVICILPNMENVYFASYGEGDNLDIYVARKLPGGKFGLPQKLNNEVNT
                     PYDENFPFMHPNGRDLYFSSKGHNSMGGYDIFKARYDADNNSYSNVANIDFAISSPDD
                     DMLYIVDNDYKNAYFSSTRQSKDGNIVVYKVGVDRIPIQIAIVKGAFQSSVLPDNPQM
                     TCVVTDKGSGKQIGVFKSQEKNSYLIVFPKGGKYNYKITVNGSNEEFNLEVDIPFLKE
                     LRPLKQKMEHLVVNGQEQIKIINLFDEQIEDAQSIVSQVLKERANLNVNEDQFNQEEL
                     NAQLDLQRKIGEIGLGNRTPNEVAQLLDSKINDASNSNQADKKIETATNGVVAQLYAQ
                     IEQTDATIKRQIIEADNADNVQRKTAILENVQEAFEQRDQLLEKIEETQKVSAQLGNQ
                     SISGDRINVKQLQQVNEQYKAALAQNNLKDASKILSDNKDFLLSTVRTNNSSITEELA
                     QQQMALDEKLKQLNALEASTAENIAVIEKSISDLTAQKDAAKEKQKAPIQDEIDAKTF
                     ELNDAKSSLEKIRTQIGEKKEEKTKHEQVIGIASEITSYNGKEVSKEEIAQAKTNANN
                     SKINTLRSYAENSLAELKNNKTTITKTFADNAIEQYNAQTEAIYANENLSDYERQKQL
                     NDLNTQQQKALTDYLAELQTKEQTDSILVQKEKIEKTLLELEQDKKIVENALATNVQK
                     EIEKLNINDIIAQVSPSYFENQTAIQQNTTLSEKEKLEQLNQNDHELINRLLDETATT
                     KEQLAQTTEKKVLQSKLQLLDNQITASKTAIAGRVKEIEKIKDQELIASKEQKTQAEI
                     DAIAQTADDAYQKSMIELQGSLDATTNIANLEQLQKELN"
BASE COUNT         2187 a         1014 c         1043 g         1591 t
ORIGIN      
        1 cagatgttaa atgcgaggaa gcctatttaa cctacattcc acgtaaaaaa atattaaagg
       61 caagttctat gcctcgcagt gagtttgaat tcttcaataa agaagcaaat ttaaagggca
      121 caactatagt tacgcctaac ggaatggaag gaaatggatt gatgactttt aaaacagcaa
      181 ctatcgtttc aacgaagttt aaatacgagc acaaagacat ttttgcagat acatccagtt
      241 tcaatcttag aaaccaaagt cgtgaagaag gtgaagcgcc cttgattttt gaaaccaata
      301 atgttaaatc gcacgttagt tttaaagaac gcaaagggga gttcaattca aataaaggag
      361 aatcacgtgt ggatttccct gtcaaccaat acatgtgtaa gatggataaa ttcacatggt
      421 ttatggacca agaagagtta acaatggaaa aacaagcaac taaagatgtg gccatcaacg
      481 ctggtgtgga tttattagga cccaacttct tttcaactca tccaaagcaa gattcattgc
      541 aatttagagc gccaaaagct aaattggaca tgaaagccaa aacaatttat tgtaatgaag
      601 tagaatacat tgatgttgcc gatgcgcgta tttatccaga tagcatgaaa gtaaatattc
      661 gcaaaaaagc taaattggat caatttacaa atgcaaccat tattgccaac tacatcacta
      721 aataccataa atttacaaaa gcggtggtgg atatcactgc tcgccgtgcc tatactggtt
      781 cgggtgaata tccatactat gatcgcgaca gtatattgtc gaacgtggtt atgaattcaa
      841 taactttgga ttcaacattt caaactgttg ctaatggaaa aattgaacaa aacgcaggct
      901 tcaaattaag tcccgagttt gattattacg gcaatatcac tgtaaaagct gcttctccag
      961 aaatcttgtt taacggtgca acacgtatca accacaactg ttctaacttt gagaaaaact
     1021 ggttggcatt tacaagtaat gtggatccga aaaatgtaca aattccagta agctccacca
     1081 tgaccaactt ggaaaatcaa gctatttcag cgggtattgt ttggagagat gccgcaaatg
     1141 tggatagcat tgccttgtat cctactttct tgtcaaaatt ggtgagtcca accgacccaa
     1201 ttgttgcaac atcatcagga tttttaatgt atgacaattc aaccaaagaa taccaaattg
     1261 catccaaaga aaaattgatt aaccgttcgg caaaaggcaa ttttctttca atgaatacag
     1321 aaacatgtgc gttgtttgca gaaggaatca tcaacttagg aatggatcat ggtgatgtaa
     1381 cggtgagcac tgctggaatg gttgactaca actcaaacac aggtgaagca ttgttcaatt
     1441 taacggctaa gtttgatatt cctattgaca aatccatctt gaaaaacatt ccagaaaaaa
     1501 ttaatttaac agaaggattg aaaccaatgg atttcaccac caacacgctt aaatcttcaa
     1561 ttacagcatg gggcacacaa gccgaagcgg ataaattgca agaaaattac acacttaaag
     1621 gcgaagtgaa aaacattcca aattgtttgg agtcaacttt caccattaca ggtattcgat
     1681 tgaattatta cagccaaccc aaattaaaca acttcaaagg gttgattacc acttcagaat
     1741 cggcaattct tgttaacatg tttgacaaac cagtgatgaa atacattcca ttcaaagcct
     1801 tcttccaaca aaaatattct ggagcaggtg gtgattggtt tgcattctta attgacattc
     1861 caggaggtaa agattatttc ttcaactatt caatgggtaa aaaagaagga atgatggata
     1921 ttatgactgg agatacagaa ctttcaactg aaatagctgc attaaaagaa gacaaacgta
     1981 aaaagaaaaa ctttacatat caaattggac gcggtggttt aaaaacagtg ttctataatt
     2041 tatttggcaa ctaatggaat ttatatattt tggattaatc gcttatttaa taggttctat
     2101 tccaactgct atttgggttg gaaaaattaa atacggtgta gatgtaagag aacacggaag
     2161 taaaaacgct ggagccacca atacgtttcg ggtgttgggg aaaaaagcgg gaatttttgt
     2221 gttaaccgtt gatattctca aaggatttat tgcttcgttt ctaccctatt tgtttatcga
     2281 tgaaaactta aaccccgaca aattggcgtt ggtgcaaatt acttgttcat ttttagcggt
     2341 gttgggacat gtttttccca tctatgcagg tttcaaaggt ggtaaaggcg ttgcaacatc
     2401 tttgggagtc attattggag tattgccaat tgctgcgttg atttgcatta ttgtattttt
     2461 aattgttttc attgcatcaa aatacgtttc attgggagca atttgtgcat cggcaacgtt
     2521 tcctattgtt acaatttttg tttttcaatc caattccatt tggttaaaag cattttcaat
     2581 tgtgcttgca gctgttgtta ttattgccca tcgaaaaaat attaaacgtc ttttagatgg
     2641 aaatgaaagt aaaatgaacc tttttaagaa ataagcgtat aagagtatgt tgcaataaaa
     2701 ctttaaatac aaacggataa agtgctgatt ataaaacata gtatactttt tgtttttaca
     2761 cttttaattg tttttgtttc atcggcgcaa accgaagctg aaatgaaagc aaaagcagat
     2821 gcttttttcg taaacaatca gtttgttgaa gccactccac tctatttgcg tttactttca
     2881 ttgcaacctc gtgattacga atacagttac aaatacggag cttgtttatt gtacaattcc
     2941 aataacaaac aagatgcctt acgttattta acctatgccg ttaaaaatcc tgtaaacact
     3001 gccgaaccgc attatttttt agggaaagct taccatctga actatcaatt caacgaagca
     3061 ataactgaat acaaaagcta tttgcaattg gcaggcacca aggctaccta ttcaaaagat
     3121 gcacaacgca atatcgaaat gtgtgcaaat ggtaaacaat taatcagcac catcaccgac
     3181 atagttgtaa aaaagaaaac agaaatcaat caaaatgatt ttttccgttt gtatgactta
     3241 tctgacattg gaggaacact gattacagcc accaattttc aaacaaaaaa cgataagaaa
     3301 ttcaaccata cgcctgtgat ttgcatttta cccaatatgg agaatgttta ttttgcaagt
     3361 tatggtgaag gtgataactt ggatatttat gttgcacgaa aactgcccgg tggaaaattt
     3421 ggtttgcctc aaaaattaaa caacgaagtg aacacacctt atgatgaaaa tttcccgttc
     3481 atgcatccca atggaagaga tttgtatttt tcatccaaag gtcataactc aatgggtgga
     3541 tacgatattt tcaaggcaag atatgatgcc gacaataatt cgtatagtaa cgtagccaac
     3601 atagattttg ctatcagttc acccgatgac gatatgctat acattgttga caacgattat
     3661 aaaaatgcgt atttctcatc cacccgtcaa tcaaaagatg gaaatattgt tgtttataaa
     3721 gtaggtgttg atagaattcc gattcaaata gccatcgtaa aaggtgcttt ccaatcgagt
     3781 gttttgcccg acaatcctca aatgacttgc gttgttacgg ataaaggaag cggaaaacaa
     3841 attggtgttt ttaaatcgca agaaaaaaat tcctatttaa ttgtattccc caaaggtgga
     3901 aaatacaact acaaaattac tgtcaacggc tccaacgaag agtttaactt ggaagtggat
     3961 attccatttt taaaagaatt acgcccattg aagcaaaaaa tggagcattt ggttgtaaat
     4021 gggcaagagc aaattaaaat catcaattta tttgacgaac aaatagaaga tgcacaaagc
     4081 atcgtatcgc aggttttgaa agaacgtgcc aatttgaatg tcaacgaaga tcaatttaac
     4141 caagaagaat taaacgccca gttggattta caacgaaaaa ttggtgaaat cggcttgggt
     4201 aatagaactc caaacgaagt tgcccaatta ttagacagta aaattaacga tgccagcaat
     4261 tccaatcaag ctgacaaaaa aatagaaact gcaaccaacg gagttgtagc tcaattgtac
     4321 gctcaaattg aacaaacaga tgcaaccata aaacgtcaaa ttattgaagc cgataatgcg
     4381 gacaatgtgc aacgaaaaac agccatcctt gagaatgtgc aagaagcatt tgaacaacgc
     4441 gatcaattat tagaaaaaat tgaagaaaca cagaaagtga gtgcgcaatt gggcaatcaa
     4501 tccatttcgg gagatcgtat caatgtgaag cagttgcaac aagtaaacga acaatacaaa
     4561 gctgcgctgg cgcaaaacaa tttaaaagat gcctcaaaaa ttttgagcga taacaaagat
     4621 tttttacttt ctactgttag aaccaacaat tcatccatca ccgaagaatt ggcacaacaa
     4681 caaatggctt tggatgaaaa attgaagcaa ttgaatgcat tggaagcttc tactgcagaa
     4741 aatattgcag taattgaaaa atccatttct gatttaactg ctcaaaaaga tgcagcaaaa
     4801 gaaaaacaaa aggcgcccat tcaagatgaa attgatgcca aaacatttga attaaacgat
     4861 gctaaaagtt cattagaaaa aattagaact caaattggtg agaaaaaaga agaaaaaacc
     4921 aagcacgaac aagttattgg aatagcaagt gaaattacgt cctataacgg aaaagaagtc
     4981 agcaaagaag aaattgcaca agcaaaaaca aatgccaata atagtaaaat caatactttg
     5041 cgcagttatg cagaaaattc attggctgag ttaaaaaaca acaaaaccac tattacaaaa
     5101 acgtttgccg acaatgccat tgaacaatac aatgcacaaa cagaagcaat ttatgccaac
     5161 gaaaatttaa gcgattatga acgccaaaaa caactcaacg atttaaacac tcaacaacaa
     5221 aaagcgctca ccgattattt ggctgaattg caaacaaaag aacaaaccga tagcatttta
     5281 gtacaaaaag aaaaaattga aaaaacactt cttgaacttg aacaagacaa aaaaattgta
     5341 gaaaatgcat tggctacaaa cgttcaaaaa gaaattgaga agttaaacat caacgacatt
     5401 attgcacaag taagtccatc gtattttgaa aatcaaacag caattcaaca aaatacaacg
     5461 ttaagtgaaa aagaaaaatt ggagcaattg aaccaaaacg atcacgagtt aatcaaccga
     5521 ttattggatg aaacggctac aaccaaagag caattggcac aaacaactga gaaaaaagta
     5581 ttgcagtcaa aactacaact actcgataat caaataacag cttcaaaaac agccattgct
     5641 ggtcgcgtaa aagaaattga aaaaattaaa gaccaagagt taatcgcttc aaaagaacaa
     5701 aaaacacaag ccgaaattga cgccattgct caaactgccg atgatgctta tcaaaaatct
     5761 atgatagagt tgcaaggttc tttggatgct acaaccaata ttgccaattt agagcaattg
     5821 caaaaagaat tgaac
//
LOCUS       DAOQVQ010000107         5827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15154_length_5827_cov_3.134615, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000107 DAOQVQ010000000
VERSION     DAOQVQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15154_length_5827_cov_3.134615"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKN22_04620"
     CDS             complement(<1..495)
                     /locus_tag="PKN22_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS42021.1"
                     /translation="MNNNNRYAVIMAGGVGSRFWPLSTPERPKQFIDVLGIGKTLIQM
                     TYERLLNIVPEQNIFVLTNQSYVEMIQQQLPNMERNRILTEPMRKNTAPCIAYAAAKI
                     QLLDRDACMIVCPSDHLIMKDDEFKNTIEKAVLNAEQNDKIVTLGIKPTRPDTGYGYI
                     EFDKT"
     gene            complement(485..1111)
                     /locus_tag="PKN22_04625"
     CDS             complement(485..1111)
                     /locus_tag="PKN22_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HNS42022.1"
                     /translation="MDKTTKIKKVLTKYVPNETEDYLVSLFALYPVSFKIVAPRKTKL
                     GDFRIISSTSIPQITVNGDLNEFAFLVTTIHEFAHLKTWMEHKNRVKPHGTEWKKNFI
                     QLMLPIIELKILPKDVEIALINSFANMKASSCSDIQLNRTLKKYDKFAESEVTLEKLP
                     KYANFTLGVRAYKKGELRRTRYLCTDLHSGKQYLIHKLATVEKIENEQ"
     gene            1216..1920
                     /locus_tag="PKN22_04630"
     CDS             1216..1920
                     /locus_tag="PKN22_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42023.1"
                     /translation="MLWIVFSKPEKWSVFRKRVFDEIQIIGLSSISIVAIMSVFIGAV
                     IALQIASNMDSPLLPAYTIGYITRSTTILEFSPTIMSLILAGKVGSNISSEIGTMRIT
                     EQIDALEIMGVNSLAYLVLPKIVAALIFFPILVTISMFLSICGGWIALVISDMGSTED
                     YVYGIRAFFKTFEVTYALTKSVVFAFIIVSIASFYGYYTKGGALEVGKAATQAVVTNS
                     ITVLVFNLILTQLMLL"
     gene            1920..2687
                     /locus_tag="PKN22_04635"
     CDS             1920..2687
                     /locus_tag="PKN22_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS42024.1"
                     /translation="MIEVKHLNKTFNGNQILFDINAEFHQGKVNMIIGQSGSGKSVLA
                     KNIVGLHTPDSGEILFAGKNFVTMSRQEKTEVRKEIGMLFQGSALFDSMTVEENVMFP
                     LKMFTNQTHKQRLDRANFCLDRVNLTGKNKLFPSECSGGMQKRIAIARAIAMHPKYLF
                     CDEPNSGLDPKTSIVIDGLIQEITYEYNMTTIVISHDMNSVIEIGDRVMFIYKGQKWW
                     EGDKNTILTTDNQEILDFVYASNFMKEIRENMQKKMK"
     gene            2789..3169
                     /locus_tag="PKN22_04640"
     CDS             2789..3169
                     /locus_tag="PKN22_04640"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNS42025.1"
                     /translation="MKYLFVFITSILLMLPQQGVPFSKLDVAFDQKNAETIVSYTKDK
                     VLLNILGKEGIYSKSQAVLVLKDFFNGKGTTSFNYTFKSSESETDTFAIGSLQCSSAK
                     YRITMHFKAVNSEYKIESITIEKD"
     gene            3172..4035
                     /gene="nadC"
                     /locus_tag="PKN22_04645"
     CDS             3172..4035
                     /gene="nadC"
                     /locus_tag="PKN22_04645"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNS42026.1"
                     /translation="MEFTSAQIEALNQIIDNALEEDLGDGDHSSLACVPADAIGKAKL
                     IIKDKGILAGVELAKHIFSRYDDSLKMTCFINDGARVEPGQIAFEIEGKSQSILATER
                     LVLNFMQRMSGIATQTHEIVKRIEGCDTKLLDTRKTTPGVRLLEKWAVKIGGGHNHRF
                     ALYDMVMLKDNHIDYAGGIQQAIERTNNYLKSNHKALKIEIEVRNADELQQVLAVGQV
                     DRIMLDNFTPEQIKAVLPTIPQQFEVEASGGITLDNIRAYAETGVDFISVGALTHSFK
                     SLDMSLKADFS"
     gene            4129..4210
                     /locus_tag="PKN22_04650"
     tRNA            4129..4210
                     /locus_tag="PKN22_04650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4163..4165,aa:Leu,seq:gag)
     gene            complement(4243..5325)
                     /gene="aroB"
                     /locus_tag="PKN22_04655"
     CDS             complement(4243..5325)
                     /gene="aroB"
                     /locus_tag="PKN22_04655"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNS42027.1"
                     /translation="MITLQNINCPIEIGSILDSSFSQFIQTNYAQSKIVIMVDENTHE
                     HCLDYLITSFDFLTTAEVMMLPFGEENKTLDVCMQVWEALSEYGIGRRDLIINLGGGV
                     VTDMGGFIASIYKRGIDFIHIPTTLLGMIDAAIGGKNGVDLGAYKNQLGVFRFPKALF
                     IDKAFLHSLPEKEMVNGYAEALKHALIKDVDLWEQLTEIEDLLELTNDEFLAKIATIK
                     VDVVNEDPQEKGIRKILNFGHTMGHAIEGYFLSGEPIDHGHSVALGMLAESFISVKLG
                     KLSQDDFADIEQVLLSWYPVPVIPMDDFPVIVELLYNDKKNHSGKIQCCLLEAIGSCL
                     FDQPVSEELFMDSLVYLMSKTSASLN"
     gene            complement(5420..>5827)
                     /locus_tag="PKN22_04660"
     CDS             complement(5420..>5827)
                     /locus_tag="PKN22_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42028.1"
                     /translation="DPATHTFQWINCADETPIDGATSATFTPSTSGNYKVVVSNGDCS
                     KTSSCKFVNVLGIDDLATEQLNVYPNPSETGVFNLSNVPTNATIEVLSLTGNVIYTAT
                     ASSNQHAVNLTNEANGVYILQVNGSNHTKIVKK"
BASE COUNT         1834 a         1043 c         1052 g         1898 t
ORIGIN      
        1 agttttatca aactcgatgt aaccataacc tgtgtcgggg cgagttggct ttattcccag
       61 tgttacaatt ttatcgtttt gctcggcatt tagcactgct ttttcgatgg tgtttttaaa
      121 ctcatcgtct ttcattatca aatggtcaga aggacaaaca atcatacatg catctctgtc
      181 taacagctga attttagccg ctgcataagc aatacaagga gcagtattct tacgcatagg
      241 ttctgttaaa atcctgtttc gttccatatt gggcaactgt tgttgaatca tttccacata
      301 ggattgattg gttaaaacaa aaatattttg ttcgggaaca atgttcaaca agcgctcata
      361 cgtcatttga atcaacgttt ttcctattcc caacacatca ataaattgtt tcggacgttc
      421 tggcgttgac aaaggccaaa atctgcttcc tactccaccc gccatgataa cggcataacg
      481 attattattg ttcattttca attttttcta cagttgctaa tttatgtata aggtattgtt
      541 ttccagaatg taaatccgtg cacaaatacc gtgtccgtct taattctcct tttttatacg
      601 ctcttacacc aagtgtaaag ttagcatatt ttggtagttt ttcgagtgtt acctcacttt
      661 ctgcgaattt atcgtatttt tttaacgttc tattgagttg aatatcgcta caagatgaag
      721 ccttcatgtt tgcaaatgaa ttgattagcg caatttcaac atctttaggc aaaattttaa
      781 gttcaatgat gggcaacatt aactgaatga aatttttttt ccattccgta ccatgtggtt
      841 tcactctatt tttatgttcc atccacgttt ttaggtgtgc aaactcgtgt attgttgtta
      901 ccaaaaaagc aaattcattc aaatctccgt tgaccgtaat ttgaggaatg gaagtgctgg
      961 aaataattct gaaatctcct aactttgttt ttcgtggggc aactatttta aaactaacag
     1021 gatacaaagc aaacaacgat accaaataat cttctgtttc attgggcacg tatttagtta
     1081 atactttttt tatctttgta gtcttgtcca ttaaattgga ttttttgtca gaaaatgcaa
     1141 atgaaagaaa atataacatt aatttagcac tccgtgataa aaaaactatt tgaaaatatt
     1201 ggtatctact ttcggatgtt atggattgtt ttttccaaac ccgaaaagtg gagtgtattt
     1261 agaaaacgtg tatttgatga aattcaaatc attggattga gttccatttc catcgttgcc
     1321 atcatgtcgg tatttattgg agcagttatt gccttgcaaa ttgcttcgaa tatggatagc
     1381 ccattgttgc ctgcctatac gattggatac ataactcgtt ccaccaccat tttggaattt
     1441 tcaccaacca tcatgtcgct cattttggca ggtaaagttg gctctaatat ttcttctgaa
     1501 attggaacaa tgcgcatcac cgaacaaatt gacgcattgg aaattatggg ggttaactca
     1561 ctggcatact tggttttacc aaaaattgtt gcagcattga ttttctttcc catattggtg
     1621 actatttcaa tgtttttaag tatttgtggt gggtggatag cgttagttat ttctgacatg
     1681 ggttcaacag aagattacgt ttatggtatt cgtgccttct ttaaaacatt tgaagttacc
     1741 tatgcattga caaaatcagt tgtatttgcc ttcatcatcg tttcaattgc ttcattttat
     1801 ggatattaca caaaaggagg agcattagaa gtaggaaaag cagccactca agcagtggtt
     1861 accaacagta taactgtttt ggtatttaat ttaattttaa ctcaattaat gttactgtaa
     1921 tgattgaagt taaacacctc aataaaacat ttaatggcaa tcaaattttg tttgatatca
     1981 atgccgaatt ccatcaggga aaagtcaata tgattattgg tcaatcggga agtggaaaat
     2041 cggtattggc aaaaaacatc gttggattgc acacacctga tagcggtgaa attttatttg
     2101 caggcaaaaa ttttgtgacc atgtctcgcc aagaaaaaac tgaagttcgt aaagaaatcg
     2161 ggatgttatt tcaaggctca gcactttttg actcaatgac ggtggaagaa aacgttatgt
     2221 ttccactaaa aatgtttacc aatcaaacac acaaacaacg tttggatcgt gctaattttt
     2281 gtttggaccg cgtcaactta actggtaaaa ataaactatt tccttctgaa tgtagtggtg
     2341 gtatgcaaaa acgtattgcc attgccagag ccattgccat gcatccaaaa tatttgtttt
     2401 gcgatgaacc gaactctggg ttggacccca aaacatccat tgtaattgac ggattaattc
     2461 aggaaattac ttatgagtac aacatgacaa ccattgtcat ttctcacgac atgaactcgg
     2521 tgattgaaat aggtgatcgt gttatgttta tttacaaagg tcaaaaatgg tgggaaggag
     2581 acaaaaacac cattttaaca actgataacc aagaaatttt ggattttgtg tatgcttcta
     2641 acttcatgaa agaaattaga gaaaatatgc aaaagaaaat gaaataattc aaattactta
     2701 ttttcagtac actacattgt ttcaatttaa ttttgttaac atattttaac ggcacatttt
     2761 ttgtatgaat acccgcaaat aaaacatcat gaaatatcta tttgttttca ttacctccat
     2821 tttgttaatg ttgccccaac aaggtgttcc gttttcaaaa ttagatgttg catttgatca
     2881 aaaaaatgct gaaaccattg tatcctacac caaagacaag gtgttactta atatcttagg
     2941 aaaagaagga atttactcca aatcacaagc agtgttggtt ctaaaagatt tttttaatgg
     3001 taaaggaact acttcattca attacacatt caaaagtagc gaatcggaga ccgacacttt
     3061 tgccattggt tccctacaat gtagtagcgc caaataccga attacaatgc actttaaagc
     3121 tgttaatagc gaatacaaaa tagaaagcat taccatcgaa aaagattaaa catggaattt
     3181 acatcggcac aaatagaagc acttaaccaa attattgaca acgcattaga ggaagattta
     3241 ggagatggcg accattcaag tttggcttgt gtgccagctg atgctattgg taaagcaaaa
     3301 ttaatcatca aagacaaagg gattttggca ggtgttgaat tagcaaaaca tattttttca
     3361 cgctatgatg actcactaaa aatgacgtgc tttatcaacg atggggcaag agtggaacct
     3421 ggacaaattg cctttgaaat tgaagggaaa tcgcaatcta ttttggctac cgaacgattg
     3481 gtattgaact ttatgcaacg catgagcggt attgctacgc aaactcatga aattgtaaaa
     3541 cgcattgaag gttgtgatac aaaattgttg gacacccgaa aaacaacacc tggtgtacgt
     3601 ttattggaaa aatgggctgt gaaaattggt ggtggacaca accatcgctt tgccttgtat
     3661 gatatggtga tgctaaaaga caaccatatt gattatgctg gtggcattca acaagctatt
     3721 gagcgtacca acaattattt gaaaagtaat cacaaagctt taaaaattga aattgaagtg
     3781 cgcaatgctg atgaattgca acaagtgttg gctgttggtc aggttgatcg tatcatgttg
     3841 gataatttta cgccagaaca aataaaagct gtattaccta ccattcctca acaatttgaa
     3901 gtggaagcat cgggaggaat taccctcgat aacatacgtg cttatgcaga aacaggtgta
     3961 gattttattt ctgttggagc tctcacccat tcttttaaaa gtctggatat gagtttaaaa
     4021 gctgatttta gttgaattta aatctcacaa cttgcatcaa ttttattgaa aaaaccaatt
     4081 ggagttttaa aacaaaccat taattgtata tatttgcgac gtcgcaaggc ccacgtggtg
     4141 gaattggtag acacgccatc ttgagggggt ggtgccagta ggtgtaccag ttcgaatctg
     4201 gtcgtgggca caatgtaaag agagtcaaaa tggctctctt ttttagttca aagaagcgga
     4261 tgttttactc attaaataca ccaacgaatc cataaacaat tcttcactca cgggttggtc
     4321 gaacaagcaa cttccaattg cttccaataa acaacattgt atttttcccg agtggttttt
     4381 cttatcgttg tagagcagtt ccactataac tggaaaatca tccattggaa tgaccggaac
     4441 gggataccaa ctcaacaata cttgctcgat atcggcaaaa tcatcttgcg acagcttgcc
     4501 taattttaca gaaataaaac tttctgccaa cattcccagt gccacactgt gaccatgatc
     4561 gataggttcg cctgataaaa aatagccttc aatggcgtgc cccatggtgt gtccaaaatt
     4621 taaaattttt cgaattcctt tttcttgcgg gtcttcattg actacgtcca ccttaatggt
     4681 tgcaattttt gctaaaaatt catcgttggt tagttctaat aaatcttcaa tttctgttag
     4741 ctgctcccac aaatcaacat ctttaatgag agcatgtttt aacgcttcgg cataaccatt
     4801 caccatttct ttttcgggta atgaatgcaa aaatgctttg tcgataaata atgcttttgg
     4861 aaaacgaaaa acacccaatt gatttttata tgctcctaaa tctacaccat ttttaccacc
     4921 aattgcagca tcaatcatgc ctagcaaggt agttggaata tggataaaat caatacctcg
     4981 tttataaatc gaagcaatga aacctcccat atctgttacc actcctccac ctaggttgat
     5041 gattaaatcc ctgcgaccaa tgccatattc ggaaagagct tcccacacct gcatgcacac
     5101 atccaaggtt ttattttctt cgccaaaagg caacatcatc acctcggcag tggttaaaaa
     5161 atcaaaagaa gtgattaaat aatccaagca atgttcgtgg gtattttcat ccaccattat
     5221 cacaattttt gattgagcat agtttgtttg tatgaattgg gaaaatgatg aatcgaggat
     5281 gctaccaatt tcaataggac aatttatatt ttggagtgta atcatggtgc taaataaaat
     5341 cgaagttaca aaaaaatgcg atacaaaaaa ggctgtccaa aaaatgaaca gccttcaact
     5401 attaaaatgc taattggtat tattttttta caattttagt gtggttgcta ccatttactt
     5461 gtaaaatata aacgccgttt gcttcatttg ttaagttaac tgcatgttgg ttgctcgatg
     5521 cagttgctgt gtaaataacg ttgcccgtta aagacaatac ttcaattgta gcattagttg
     5581 gcacatttga caagttgaaa acacccgttt cacttgggtt aggataaacg tttaattgtt
     5641 cagttgctaa gtcatcaatt cccaatacgt ttacaaattt acaactagat gttttagaac
     5701 aatctccgtt tgagacaact actttgtaat ttccagatgt tgatggagta aaagttgcgc
     5761 ttgttgcacc atcaattggt gtttcatctg cacaattaat ccattggaaa gtgtgcgttg
     5821 ctgggtc
//
LOCUS       DAOQVQ010000108         5817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15186_length_5817_cov_3.415481, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000108 DAOQVQ010000000
VERSION     DAOQVQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15186_length_5817_cov_3.415481"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKN22_04665"
     CDS             complement(<1..233)
                     /locus_tag="PKN22_04665"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNS42029.1"
                     /translation="MKVYTKRGDQGTTQLIGGTRVLKSSLRLESYGTVDELNSYMGLL
                     RDLIQNEELEDQILEIQDRLFTIGSHLASDPNNS"
     gene            complement(230..1930)
                     /locus_tag="PKN22_04670"
     CDS             complement(230..1930)
                     /locus_tag="PKN22_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNS42030.1"
                     /translation="MAIEHFINILIINDDQKLTAGFKEILSGSGNNVMAVNDYKEAIP
                     ILQKREVGIVLINLDSKSFVGMEILQELKNESITPNTYKILITKSNTSAAKMVRGLNS
                     GAVDFISAPFSPNLVKAKIEVYKMLFYKDQRIVQLLQNIFPTNVLEDFNTYGKFSPKR
                     VENGVVLFTDFVDFSQLSAKMEPIQLLRQLEKYFTTFDEIIARYRLEKIKTIGDAYMA
                     LAGVTENKANPAIRACLAALEIQQYIDSEAELCKALGKDYWQIRIGIHMGPLVAGIIG
                     KTKFSFDVWGDSVNIASRAERASLPGRITITDAVFENVNRYFNTTERGEVDIQKRGGR
                     VKMYFLDGINEKYLLDKRRKVANIELRNLCDLTPLDFFNMRVDIINKLKALLPEDLGY
                     HDVQHTLNVEKAAMRYGKLEGLSHLEMLLLRTAVLYHDAGFILQYDNNEDYAILLAKE
                     TLPTFGYSEEQITLICGIINATKSTVEPQTLLEKIMCDADHDYLGRVDYRVISSRLRS
                     ELEVMSNLYMSDVEWVEFQLKYLTSKHQYYTDTANNIRKNGKNVRIQELNNLLKQLQQ
                     "
     gene            complement(1937..3730)
                     /locus_tag="PKN22_04675"
     CDS             complement(1937..3730)
                     /locus_tag="PKN22_04675"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNS42031.1"
                     /translation="MKFIVFLIVFVSGFMAASFSQTARYELRMENPANHYFQVDFYLK
                     DYKSKEIEVKLPVWAPGSYLVREFSKNLNQVRATDENGNPLQVIKKTKNAWIIKREKA
                     KEVKVSYEVYAFELTVRTSFLDLTHGFVSGTGVFMYTEETRNKPGQLKIIPHASFSKI
                     STALPEVGEGVVNDSKAKEYSFENYDVLADSPIEIGNQEEFTFDAAGVKHTVAMYGWG
                     NYDVEKLKVDMAKIVTSTTAIFGENPNKNYTFIIHNVTDGQGGLEHLNSTTLSVNRYT
                     YQGDDYLGFLSLVAHEYFHLWNVKRLRAFELGPFDYDREVYTSLLWVMEGFTSYYDEL
                     ILLRAGFYDANQYLNKLKSALNYVEGTPGNSVQPVAHASFDAWIKAYRPNENSSNTTI
                     SYYSKGGVIGALFDAMIIDKTNGSKCLDHFLQTLYAKYYKKLNRGFTEKEFQDEFSNF
                     LGKDMTSFFNDHIYGTKTPDYATIFNAVGLKVEYDGRKENSIGARIAQSGASLYVKSI
                     RAESGAEKSGLSVGDEIIGCNGMRVNQSDFESLVAQLEEGKHLNLLISRDDVLMEIQL
                     RSSTYERPSYKFSAATDEKNNAKRDYWLRTE"
     gene            3847..>5817
                     /locus_tag="PKN22_04680"
     CDS             3847..>5817
                     /locus_tag="PKN22_04680"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNS42032.1"
                     /translation="MRKIALFIATTFSIFNFSIAEEGMLIPSVITAFESDMQAMGMKL
                     TAEEIYSINKSSLKDAIIHFGGGCTAELVSNQGLLLTNHHCGYSQIQAHSSLKNDYLK
                     YGFWAKTKADELPNKGLTASRMVRIQDVTKEVLAGTNGITNQDELKKILTRNTQALIT
                     EATNGNHYQAEIKPFNQGNEFYMIVKETFLDVRLVGTPPNSIGKFGGDTDNWVWPRHT
                     GDFSVFRIYAGADNKPAEYSATNVPYQPIHFLKIAFNDRKVGDFTMVYGFPGETEQHV
                     ASDYLKFIMEKERPARIRMRELSLSVIDQAMRESDEVRIKYASKQASIANAYKKWIGQ
                     IDGLRRMDAVSVKKERENVYVQRANSKPEWKNEYGAVVEQMNKLVASSVQAEFEYSMA
                     IEYFFVGPEFLKLARGVDQLRKTALNNPAGVDALKKRLLDGAEGFFKNYDAKVDQRIF
                     ELTTAAYNNYISAPSTAKINNAATNYNANVIFEKSIFTNQQRYQAFLEKFTAKSVKKL
                     LKDPGFAHFNLLYSNFIDQSLEKYEQYDAAMGDLLKTYVKGKLEMFPEQKHWADANST
                     LRITYGKLEGSEPQDGVAYLEHTTLDGVIDKYYSGNPDFELLPRMLELHKKKEYGRYA
                     QDGELWVCFTGSNHTTGGNSGSPVLDAHGNLMG"
BASE COUNT         1826 a         1125 c          983 g         1883 t
ORIGIN      
        1 gagttatttg ggtctgacgc taagtgtgag ccaatggtaa acaaacgatc ttgaatttct
       61 aaaatttgat cttctagttc ttcattctgt attaaatcgc gcagtaatcc catgtaagaa
      121 ttcaattcgt ccactgtgcc ataagattct agtcttaacg aactttttaa aacgcgtgtt
      181 ccaccaatta attgcgttgt tccttgatca cctctttttg tataaacttt cattgttgta
      241 attgtttaag taaattattt aattcttgta ttcgcacatt tttaccgttt ttgcgaatat
      301 tattagctgt atcggtataa tattgatgtt tgctcgtcaa atattttaat tgaaactcca
      361 cccattctac gtctgacata taaagattac tcatcacttc taactcactt cgtaaacgtg
      421 aagaaataac tctataatct actctaccaa gataatcgtg gtcggcatca cacattattt
      481 tctccaaaag ggtttgcggt tcaacggttg attttgttgc attaattatg ccacaaatta
      541 gtgtaatttg ttcttctgaa taaccaaaag ttggcaatgt ttctttcgct aacaaaatgg
      601 cataatcctc attgttatcg tattgcagaa taaatccggc atcgtggtac aaaacagcag
      661 tgcgcagcaa caacatttct aagtgagaaa gtccttccag cttaccataa cgcatggctg
      721 ctttttctac gtttaaggta tgttgcacat catggtagcc caaatcttcg ggtaaaagtg
      781 ctttgagttt attgatgata tctacccgca tgttaaagaa gtcgagtggc gttaaatcgc
      841 acaaattcct caattcgata ttggcaactt ttcggcgttt atccaacaaa tatttctcat
      901 taataccgtc taagaaatac atttttaccc tacctcctct tttttgaata tctacttcac
      961 ctctttctgt tgtgttaaaa tagcgattaa cattttcgaa aacagcatcg gttattgtga
     1021 tacgcccagg taaacttgct ctttctgcac gcgaagcaat attaacacta tccccccata
     1081 catcgaagga aaattttgtt tttccgatta taccagcaac caacggaccc atgtgaatac
     1141 ctattctaat ctgccaataa tcttttccta gtgctttaca aagttctgct tctgaatcaa
     1201 tgtattgttg tatttctaaa gctgccaaac aagcacgaat tgcagggttt gctttatttt
     1261 ctgttacacc tgccaacgcc atgtatgcat ctccaatggt tttgattttc tcaagtcgat
     1321 aacgagctat gatttcatca aaagtggtaa aatacttctc taattgacga agcaattgta
     1381 ttggttccat tttggcagac aattgagaaa aatccacaaa atcggtaaac aacaccactc
     1441 cgttttccac tcttttagga gaaaatttcc cataggtatt aaaatcttct aacacattcg
     1501 tagggaaaat attttgaagt aactgcacaa tacgttgatc tttgtaaaac aacattttgt
     1561 acacttcaat tttagctttt actaaattgg ggctaaaagg agcagaaata aaatcgacag
     1621 ctccactgtt caatcctcgc accattttgg ccgctgatgt gttgctcttg gtaatcagaa
     1681 ttttataggt attgggcgta atgctttcat tcttcaattc ttgcaaaatt tccatcccta
     1741 caaaggattt actgtccaaa ttgattaaaa caatcccaac ttcgcgcttt tgaagaatag
     1801 gaatagcttc tttgtaatca ttaactgcca ttacattgtt gcccgagccc gataaaatct
     1861 ccttaaaacc agctgttaac ttctggtcat cgttaataat taggatattt ataaagtgct
     1921 caatggccat aatgcactat tctgttctta accaataatc tcttttagcg ttgttttttt
     1981 catccgttgc agctgaaaac ttgtagctgg ggcgttcata ggttgaactt ctcaattgta
     2041 tttccattaa aacatcatct cgagaaatca acaaattcaa atgcttccct tcttccaatt
     2101 gagccactaa gctttcaaaa tcgctttgat taacgcgcat tccgttgcaa ccaataattt
     2161 catcgccaac actcaacccc gatttctcgg cacccgattc agctctgatg gatttaacgt
     2221 ataaactagc tccagattgt gcaatgcgtg cgccaattga attttctttt cttccatcat
     2281 actcaacttt taaaccaact gcattaaaaa ttgtagcgta atcgggtgtt tttgtaccat
     2341 aaatatgatc gttgaaaaac gaagtcatat cctttcctaa gaagtttgaa aattcatcct
     2401 gaaattcttt ttcagtaaat cctctgttta atttcttata atattttgca taaagcgttt
     2461 gtaaaaagtg atccaaacat ttgcttccgt tggttttatc aataatcatt gcatcaaaca
     2521 aagcaccaat tacaccacct tttgaatagt aagaaattgt agtgttagag ctattttcat
     2581 tgggacgata ggcttttatc cacgcatcaa aactagcatg tgcaacgggt tgaacactat
     2641 ttccaggcgt accttctaca taattcaatg cactttttaa tttattcaaa tattgattgg
     2701 catcgtaaaa tccagctctt agcaaaatca attcatcgta ataggaagta aaaccttcca
     2761 ttacccacaa taatgatgtg taaacttctc ggtcgtaatc aaatggacct aattcaaaag
     2821 cgcgcaaacg ttttacattc cacaaatgaa aatattcatg cgcaactaaa gataaaaacc
     2881 ctaaataatc atcgccctga taggtgtagc gattaaccga taatgtggta gagtttaaat
     2941 gctccaatcc tccttgtccg tcggttacat tgtgaatgat aaacgtataa tttttattgg
     3001 gattttctcc aaaaattgcc gttgttgaag tgacaatttt agccatatcc actttcaatt
     3061 tttccacatc gtaattaccc caaccataca ttgcaacggt gtgttttacg cctgctgcat
     3121 caaaggtaaa ttcttcttga tttccaattt ctattggact atcagccaaa acatcataat
     3181 tctcaaaact atattcttta gccttactat cgtttactac accctctccc acttctggca
     3241 aagcagttga aattttagaa aatgaagcat gtggaatgat ttttaattgt cctggtttat
     3301 tgcgtgtttc ttcggtgtac ataaaaacgc ctgttccgct tacaaatcca tgtgttaaat
     3361 ctagaaaact agttcttacg gttaattcaa acgcatacac ttcgtagctc acttttactt
     3421 cttttgcttt ttcgcgttta attatccacg cattttttgt ttttttaatt acttgcagtg
     3481 gattgccatt ttcatcggtt gcacgcactt gattgaggtt tttcgaaaac tcacgcacca
     3541 aatacgaacc cggtgcccaa accggaagtt ttacttctat ttctttggat ttataatcct
     3601 ttaaataaaa atctacttga aaataatgat tggcaggatt ttccattctc aattcataac
     3661 gagctgtttg agaaaaactt gcagccataa atccgctaac aaatacgatt aaaaaaacga
     3721 taaatttcat tgaaactatt ttaatttaaa aagctaaaat aataaaatat aacgtggctt
     3781 gacgtacctt ccttcaatct ttacaaacaa ttttctaact ttgttgaaat tctttatcaa
     3841 aaaaacatga gaaaaatagc attattcatc gcaactactt tttcaatttt taatttttct
     3901 attgccgagg aaggcatgct cattccttct gtaattaccg cttttgagtc ggatatgcaa
     3961 gccatgggaa tgaaattaac agccgaagaa atttatagca tcaacaaatc gagtttaaaa
     4021 gatgccatca tccattttgg aggtggttgc actgccgaat tggtttctaa tcaaggattg
     4081 ttgttgacca atcaccattg tggttactcg caaattcaag ctcattcttc gttgaaaaac
     4141 gattatttaa aatatggttt ctgggcaaaa acaaaagccg atgagttgcc taacaaaggg
     4201 ttaacagctt caagaatggt gcgtattcaa gatgtaacaa aagaagtttt ggctggaaca
     4261 aatggcatta ctaaccaaga tgaattgaag aaaattttaa ctagaaatac tcaagcttta
     4321 attaccgaag caacaaacgg aaatcactac caagctgaaa tcaagccttt taaccaagga
     4381 aatgaatttt acatgattgt aaaagaaacg tttttggatg tgcgcttggt tggaactcca
     4441 cccaattcaa ttggtaaatt tggtggagat acagacaact gggtgtggcc acgtcatacg
     4501 ggtgattttt cggtatttag aatttatgca ggtgccgata ataaaccagc agaatacagc
     4561 gctacaaacg taccctacca acctattcat tttttaaaaa tcgctttcaa cgatcgtaaa
     4621 gtgggtgatt ttacaatggt ttatggtttt ccgggtgaga cagaacaaca cgttgcttcg
     4681 gattatttga aattcatcat ggaaaaagaa cgtccggcac gtattcgtat gcgtgaactt
     4741 tcattaagtg taattgacca agcgatgcgt gaatcggatg aagtgcgcat taaatatgca
     4801 tcaaaacaag ctagtattgc caatgcttac aaaaaatgga taggccaaat tgatggtttg
     4861 cgccgcatgg atgccgtaag tgttaaaaaa gaacgtgaaa atgtgtatgt tcaacgtgcc
     4921 aattctaaac cagaatggaa aaatgaatac ggcgcagttg tggagcaaat gaacaaatta
     4981 gttgcatctt ctgtgcaagc tgagtttgaa tattcgatgg caattgaata cttttttgtt
     5041 ggaccagaat ttttaaaatt agcacgtggt gtggatcaat tgcgtaaaac agctttgaac
     5101 aaccctgctg gagtggatgc cttaaaaaaa cgtttgttag atggtgctga aggttttttc
     5161 aaaaactacg atgcaaaagt ggatcaacgc atttttgaat taacaactgc tgcgtataac
     5221 aactacatca gcgcgccttc aacggctaaa attaacaatg ctgcaactaa ttataacgca
     5281 aacgtcattt tcgaaaaatc tatttttacc aatcaacagc gttaccaagc atttttagag
     5341 aaatttacag caaaatcggt gaagaaatta ttaaaagacc ctggatttgc acatttcaac
     5401 ttactgtatt ctaacttcat tgaccaatca ttggaaaaat acgagcaata cgatgcagct
     5461 atgggggatt tattgaaaac ttatgtaaaa ggtaaattag aaatgttccc agaacaaaaa
     5521 cactgggcag atgccaacag cactttgcgc attacttatg gtaaactgga aggttctgaa
     5581 ccgcaagacg gtgttgcata tttagagcac actactttgg atggggtgat tgataaatac
     5641 tattctggaa accctgattt tgagttattg ccacgcatgt tggaattaca caagaaaaaa
     5701 gaatatggca gatatgcgca agacggtgaa ttgtgggtgt gtttcacagg atctaaccat
     5761 acaactggcg gaaattcggg ctcacctgta ttggatgcac acggcaattt aatggga
//
LOCUS       DAOQVQ010000109         5809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15218_length_5809_cov_4.028328, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000109 DAOQVQ010000000
VERSION     DAOQVQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15218_length_5809_cov_4.028328"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKN22_04685"
     CDS             complement(<1..539)
                     /locus_tag="PKN22_04685"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNS42033.1"
                     /translation="MQDTFKHKGMRRQLINELREKGIKNEAVLEAFDAIPRHFFLDLV
                     FEQQAYSNQAFQIGAGQTISHPYTVAFQTDLLELKKGEKVLEIGTGSGFQTCILCQLG
                     AKVFSIERQRELFLKAQKIIHHFNFRPRLMYGDGYAGMPSFAPFDKILVTCGAPDVPQ
                     ALLEQLKVGGIMVIPVGEG"
     gene            complement(658..1431)
                     /locus_tag="PKN22_04690"
     CDS             complement(658..1431)
                     /locus_tag="PKN22_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNS42034.1"
                     /translation="MEIIVSDNNGVRTLKLNRPEVFNSFNQKMAFALHQELDKAEADN
                     NVRAIVLIGEGKAFCAGQDLAEVMDPNGPELTSIVANHYNPIIERIRKIEKPIIGAVN
                     GVAAGAGANIAIACDITIAKKSAAFIQAFSKIGLIPDSAGTFFLPRIIGFQKAAALMM
                     IGDKVSAEDAAAMNMIYKAVDDENFEAEVYKLAEKLAQMPTKGLGLTKRALNLSMTNN
                     LTQQLAVEGELQTLAGQTYDYNEGVNAFLEKRTPVFKGE"
     gene            complement(1444..2604)
                     /locus_tag="PKN22_04695"
     CDS             complement(1444..2604)
                     /locus_tag="PKN22_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNS42035.1"
                     /translation="MNKFIFSLLFAGVSFVSCTQTQTTVNTNKVEKTVIEKPVVNVLE
                     NVTIDDYFSTNEQIDAEVKNVFSKLSDEEKVAQLIMPAMGRLGLTEEAIMALVNQKKI
                     GGVLMLNGTKEQFTQWITKINEKNKELGAMPFLFSADAEPSLVNRKIAGSTIVKKAAE
                     LLTEEDVRLVAQQISKDLNDIGINYNFAPVVDMSTNSTVGYRGFGKNPKNIVPFSNAF
                     IEESEKANIITTAKHFPGHGLVSGDTHKSLQVTDGELKELDNFKALIKSNVPSIMIGH
                     LAVKNNPKYNTNGLPATVSPEIVTNLLRNELGYKGLVVTDAMNMGGVTQVPNANAAVI
                     AAGGDIVLMPLDASKAHAEILKKYQSDEAFKKIADEAVKRVLRMKICMKLKN"
     gene            complement(2597..3625)
                     /locus_tag="PKN22_04700"
     CDS             complement(2597..3625)
                     /locus_tag="PKN22_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS42036.1"
                     /translation="MDSLTQIVLGAAVGEAVAGRKMGAKAALWGAIAGTIPDLDVFIT
                     AFYHPIEGALLHRGFSHSILFPLVFAPLFAWLTHLLYKRKYEYRLWVLLFFLGIITHP
                     LLDIFTNYGTSLFWPLDTRVAINSVYVIDPLYTLPFLITVLLAICSKKDAKWRSFINW
                     AGIIYSSLYLIWGLLVQTSIKNNAQSYFAQSGVKAERITVNAMPLTSFYWMILGEDKQ
                     HYYITYKSIFGNYNPKDVEVIDKNHQLLRALKWKKEQANYPQLLRRFSKDYYTLIKGN
                     NYYDFYDLRFGTTTKLTNGVVSKPIYGFKLIVDNDFVNKTARVDNEKAFSSINFGAYW
                     NYIFAKNE"
     gene            3804..4454
                     /locus_tag="PKN22_04705"
     CDS             3804..4454
                     /locus_tag="PKN22_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNS42037.1"
                     /translation="MANVINTILQFLVPKENKFIPLLKGASTNLVEVSEQLDELVNAP
                     KGEREDLQEKLTKLDIESDQIMRSINLELSRNFLTPFDREDIHALNIAIGDVVDYLND
                     AANRMIIYQVDDVTKSIKKLTNVNIEGCKLIAKGIDCLAGKKKLTHIAVICKKIYELE
                     VKADKIHDKSIAEIFEKETDAKTIIKYKEVLSALETATDKCKAVSDILESIMVKYS"
     gene            4467..5519
                     /locus_tag="PKN22_04710"
     CDS             4467..5519
                     /locus_tag="PKN22_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNS42038.1"
                     /translation="MELLITIIVLAFIFDYINGFHDAANAIATIVATKVLTPFQAVLW
                     AAFFNFVAYFISLYIIGEFKIGNTIAKTVFEDYITLEVIFAGLVAAIIWNLATWKLGI
                     PSSSSHTLIGGFMGAALVHAYTTYGIVDGVINVVKVFPIVIFIFVAPFLGLIVAYFIT
                     VLIMFICRKTTPHKADVWFRRLQLVSSALFSMGHGANDAQKVMGIIGAAVIFYHVQAG
                     DTEWINAPDRFAHFVEEWWWVPLTSFIVIGLGTMSGGWKIVKTMGSKITKVNSLEGVS
                     AETSGAITLYISEHFGIPVSTTHTITGSIIGVGITRRISAVRWGVTVQLVWAWILTIP
                     VSAFIAGITYLIISQF"
BASE COUNT         1815 a         1073 c         1001 g         1920 t
ORIGIN      
        1 tttccctctc caacaggaat caccatgatg ccgcctactt ttaattgttc aagcaaagct
       61 tgtggtacat caggcgctcc acaagttacc aaaattttat caaacggtgc aaaacttggc
      121 attccagcat aaccatcacc atacatcaaa cgtggtctga agttaaagtg gtgaatgatt
      181 ttttgtgctt tcaaaaacaa ttcgcgttga cgttctatgg aaaacacctt agcaccaagt
      241 tggcacaaaa tgcatgtttg aaaaccagaa cccgtaccaa tttccaatac tttttcacct
      301 tttttcaact ccaataaatc ggtttgaaaa gcaacggtat agggatgaga aatggtttgt
      361 cctgcaccaa tttgaaatgc ttgattgctg tatgcttgtt gttcaaaaac taaatctaaa
      421 aagaaatgac gtggaatggc atcaaacgct tctaaaacag cttcgttttt aattcctttt
      481 tcacgcaact cgttgatgag ttgtcttctc attcctttgt gtttgaaagt atcttgcatg
      541 gtgcaaagtt agtaaactat tattgatttt atggtatgtt ctgatgattc gtaagacaaa
      601 aaatcgcaat atcgacatat ttcgatatca cgatttcttg aaaaattttt cgagtaatta
      661 ttctccttta aaaacaggtg ttcttttttc taaaaatgca ttcacacctt cattgtaatc
      721 ataggtttgt cctgccaatg tttgcaattc tccttcaact gccaattgtt gtgtcaaatt
      781 attggtcata cttaagttca atgctctttt ggttaaacca agacctttag ttggcatttg
      841 cgctagtttt tcggctaatt tatagacttc tgcctcaaag ttttcgtcgt caacagcctt
      901 ataaatcatg ttcatcgctg cagcatcttc ggcacttact ttatcaccta tcatcatcaa
      961 agcagctgct ttttgaaaac caatgatgcg tggcaaaaag aacgttccgg cagaatctgg
     1021 gattaatccg atttttgaaa acgcttgaat aaacgctgca cttttcttag caattgtaat
     1081 atcgcaagca atggcaatat ttgcaccagc accagccgct acaccgttta cagctccaat
     1141 aattggtttt tcaattttgc gaatacgttc aatgattggg ttgtaatgat tggcaacaat
     1201 agaagtcaac tctggaccgt ttggatccat tacttctgct aaatcttgac ctgcacaaaa
     1261 tgctttcccc tcaccaatta aaacaatagc acgcacatta ttatctgctt ctgctttgtc
     1321 caattcttgg tgcaaggcaa aggccatttt ttgattaaac gaattgaata cttctgggcg
     1381 gttcaatttt aaagttctaa cgccgttgtt atccgataca ataatttcca tagttgttct
     1441 attttagttt tttaatttca tgcaaatttt catacgcaac acacgtttca ctgcttcatc
     1501 agcaattttc ttaaacgctt cgtctgattg gtattttttc aaaatttctg cgtgtgcttt
     1561 actggcatcc aaaggcatta aaacaatatc gcctccagca gcaataacag ccgcattagc
     1621 atttggcact tgagttactc cacccatgtt cattgcatca gtaaccacca aacctttata
     1681 gcctaactcg tttctcaata aattggttac aatctctggc gatacggttg ctggcaagcc
     1741 attggtattg tattttggat tatttttcac tgccaaatgt ccaatcataa tggatggcac
     1801 gttggattta attaaagctt taaaattatc caattctttt aactcaccgt cagtaacttg
     1861 caacgattta tgggtgtctc cagaaaccaa tccatgacca ggaaaatgtt ttgccgttgt
     1921 tatgatgttg gctttttcag attcttcgat aaatgcattg gaaaaaggaa caatgttttt
     1981 cggatttttt ccaaaacctc tgtatccaac tgtagaattg gttgacatat ctacaacagg
     2041 tgcaaaattg taattgatac caatatcgtt caaatccttt gaaatttgtt gcgccaccaa
     2101 gcgcacatct tcttcggtta acaattcggc tgctttttta acgatggtag aacctgcaat
     2161 tttacgattg accaaagacg gctcagcatc agcactgaac aaaaatggca ttgcgcctaa
     2221 ttctttattt ttttcgttga ttttggttat ccattgtgta aattgttctt tcgttccgtt
     2281 aagcataagc acgccaccaa ttttcttttg gttaaccaac gccatgattg cttcttcggt
     2341 taaccccaaa cgccccattg ctggcatgat taattgcgcc actttttctt cgtcggataa
     2401 ttttgagaaa acatttttaa cctcggcatc aatttgctca ttcgtagaaa aataatcgtc
     2461 gatggttaca ttctccaaaa cgtttacaac cggtttttca attacagttt tttcaacttt
     2521 gttagtatta actgtagttt gtgtttgtgt gcatgacaca aaagacactc ctgcaaatag
     2581 caaggagaaa ataaatttat tcattttttg caaaaatata attccaataa gcaccaaaat
     2641 taatgctaga aaatgctttt tcattgtcaa cacgtgctgt tttattcaca aaatcattgt
     2701 caacaatcaa tttgaagcca taaattggtt tagaaacaac gccattggtt aatttggttg
     2761 tagttccaaa gcgcaaatca taaaaatcgt aataattatt gccctttatt aatgtgtagt
     2821 aatcttttga aaaccgtcgc aataattgag gataattagc ttgttctttt ttccatttca
     2881 aggcgcgtaa tagctggtgg tttttatcaa tcacttcaac atccttagga ttgtaattgc
     2941 caaaaattga tttataggtg atgtagtaat gttgtttgtc ttcacccaaa atcatccaat
     3001 agaaagaagt taaaggcatg gcatttactg ttattctttc agcttttaca cccgattgcg
     3061 caaaatatga ttgggcatta ttttttattg aagtttgcac cagcaatccc caaattagat
     3121 acaaggatga ataaataatt cctgcccagt taatgaatga acgccatttt gcatcttttt
     3181 tactacaaat tgcaagcaaa acagtgatta aaaatggaag ggtataaagt ggatcaatga
     3241 cataaaccga gttgattgca acacgggtat ccaaaggcca aaacaaactt gttccgtaat
     3301 tggtaaaaat atccaacaaa ggatgggtaa taattcctaa gaaaaaaagc aaaacccaca
     3361 aacggtattc gtattttcgt ttatacaaca aatgcgttaa ccaagcaaaa agaggcgcaa
     3421 aaactagtgg aaacaagatg gaatgagaaa atcctcggtg taacaatgcc ccttcaatag
     3481 gatggtaaaa agccgtgata aaaacatcca agtcgggaat agttcctgca attgctcccc
     3541 acaatgctgc ttttgctccc atttttcgcc cggcaacagc ttcaccaacg gcagctccga
     3601 gtacaatttg cgttaaagaa tccatttatt aaaaaaaaga taaaattaga aacattttag
     3661 gaagtcattt gaacaaatgg aagaaatgga agaaatctaa actataataa ggagatttaa
     3721 caagaaaatt aacagttttt aaaataaaac atccgtaaat cgtaaagaat ttaatatttt
     3781 tgtgtcgaat ttatttcaaa tatatggcaa acgtaattaa taccatccta cagtttttag
     3841 ttcctaaaga aaacaagttt attccattac ttaaaggagc ttccaccaac ttagttgaag
     3901 tttctgaaca attagacgaa ttggtcaatg caccaaaagg agaacgtgag gacttacaag
     3961 aaaaacttac aaaactagat attgaatcgg atcaaattat gcgtagcatt aatttagagt
     4021 tgagtagaaa cttcttaact ccgtttgacc gcgaggatat tcacgcatta aacattgcta
     4081 ttggtgatgt tgtggattat ttgaacgatg cagccaacag gatgattatt taccaagtag
     4141 atgacgttac taaatcaatt aaaaaattaa ctaacgttaa tattgaaggc tgtaaattaa
     4201 ttgctaaagg aattgattgt ttggctggga aaaagaaatt gacccacatt gctgtaattt
     4261 gtaagaaaat ttatgaattg gaggtgaaag ccgataaaat tcacgataaa tcaattgcag
     4321 aaatctttga aaaagaaaca gatgctaaaa ccatcattaa atacaaagaa gtgctttctg
     4381 ctcttgaaac agcaaccgac aaatgcaaag ctgtatcgga tattttagag tcgataatgg
     4441 ttaaatactc ctaaaattag tcccacatgg aattactaat taccatcata gttcttgcat
     4501 ttatttttga ctatatcaat ggttttcacg atgccgccaa tgcaattgct acgatagttg
     4561 ccacaaaggt attaacacca tttcaagcag ttttatgggc tgcatttttc aactttgttg
     4621 cttatttcat ttcgctttac atcattggtg aatttaaaat tggtaacacc atagccaaaa
     4681 ccgtatttga agattacatc accctagaag taatttttgc aggtttggtg gccgctatta
     4741 tttggaattt agccacttgg aaattaggaa ttccttcctc ttcatcccac acattaattg
     4801 gtggttttat gggagcagca ttggtacacg cttacacaac ttatggaatt gttgatggtg
     4861 ttattaacgt tgttaaagta tttcctatcg ttattttcat ttttgtagca ccttttttag
     4921 ggttaattgt tgcttatttc attacggtac taataatgtt tatttgccgt aaaacaacac
     4981 cccacaaagc cgatgtttgg tttagaagat tacaacttgt ttcatctgcc ttattcagta
     5041 tgggacatgg tgccaatgac gctcaaaaag taatgggtat cattggagca gctgtgattt
     5101 tttaccacgt tcaggctgga gacactgaat ggatcaatgc acccgataga tttgctcatt
     5161 ttgttgaaga gtggtggtgg gtacctttaa cttctttcat tgtcattggt ttgggaacta
     5221 tgagtggtgg ttggaaaatt gtaaaaacaa tggggtcaaa aatcacgaaa gtaaactcat
     5281 tggaaggtgt gagtgccgaa acttctggtg caataacgct ttacatttct gagcattttg
     5341 gaattcctgt ttctacaaca cataccatta caggaagtat cattggtgtt ggtattacac
     5401 gccgtatttc ggctgttcgt tggggagtaa ccgtgcaatt ggtttgggcg tggattttaa
     5461 caattcctgt ttctgcattt attgctggaa tcacctattt aatcatctct caattttaat
     5521 ttacagttta aaaaatagct tattgttttg caattagtgc aataatggtg atgattgaat
     5581 gccgtttatt tgcagactgc attatggatg tctatacgtt aattgccact ttcctttatt
     5641 caagctatcc aaaagacaga cacaatcctg ttcaaattgc aaaatgcctt gtgctaaaat
     5701 aggttttcac aattatttga tttataagca tgcaaaaacc gaaataaagt aaagaatttt
     5761 aggacttcat ttaccctaca aaaaaaggtg cctttatcag acacctttt
//
LOCUS       DAOQVQ010000110         5780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15315_length_5780_cov_4.120000, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000110 DAOQVQ010000000
VERSION     DAOQVQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15315_length_5780_cov_4.120000"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKN22_04715"
     CDS             complement(<1..382)
                     /locus_tag="PKN22_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HNS42039.1"
                     /translation="MKILVGISKSPDTTSKIAFTDGNTKFDENGVQYIVNPYDEWYAL
                     VRALELKETLGGSVTIVTVGAAADEAVIRKALAIGADDAVRIDADPKDAYYTAMQIAE
                     YAKDKGFDLVLTGKETINYNGSQVG"
     gene            624..908
                     /locus_tag="PKN22_04720"
     CDS             624..908
                     /locus_tag="PKN22_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNS42040.1"
                     /translation="MSEKKNNDNIDKANFHFGFRKENYKLLFIGLAINILGYILMIGG
                     GSKDPNVFTGDQLFNTTRLTIAPILIIAGFVIMIYSIMKKSKSSNSTSEE"
     gene            914..1708
                     /locus_tag="PKN22_04725"
     CDS             914..1708
                     /locus_tag="PKN22_04725"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNS42041.1"
                     /translation="MNFIEAIILAIIEGLTEFLPVSSTGHMIIGSSLMGIADQDFTKL
                     FTIVIQLGAILSVVVMYFKRFFQSINFYIKLLVAFIPAAVFGVLLSDKIDELLESPTT
                     VAISLVIGGIILLFVDDWFKNPTVNEEKEISYLTALKIGFFQCLAMVPGVSRSAATIV
                     GGMSQKLSRSAAAEFSFFLAVPTMLGATSKKLLDYFQDGNSFTSEQINLLIVGNIVAF
                     IVAFIAIKGFISFVSKRGFKAFGIYRIVVGLLILILIWSGFDLKVV"
     gene            1708..2403
                     /gene="truB"
                     /locus_tag="PKN22_04730"
     CDS             1708..2403
                     /gene="truB"
                     /locus_tag="PKN22_04730"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNS42042.1"
                     /translation="MAYDFSAGEILLIDKPYKWTSFDVVNKLRWNLRKKYGIKKFKVG
                     HAGTLDPLATGLLVICTGKATKKIPDLTNDDKAYTGTFLIGKTTPCFDLEKPFDDKIY
                     PTEHITDELMQSVVQSFLGVQDQYPPIFSAKQIDGKRAYDLARAGKEVEMKPNQIEIK
                     EFTISTERFPEVDFYVKCSKGTYIRSIANDFGVKLNSGATLIALNRVQSGDFLLENAK
                     RVDEWIALINEEN"
     gene            complement(2400..3245)
                     /locus_tag="PKN22_04735"
     CDS             complement(2400..3245)
                     /locus_tag="PKN22_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42043.1"
                     /translation="MKHLIFIALILWGYIGWAAPIDITEAIATGKIKVLTSYEKLGNN
                     GVILTLTNLQASPITIVVPAGTLFKSSDEEQELLNIEEQLFVLQQNEQKKVRVGGYCT
                     QLKRIAPRLDSKFTIGKTKNTLLLAFLEFLKTNQPSKSNYQEAVWALTDKEPIESIEV
                     KNDADTKLREFIAKKTNRTNPWYYKQQSVGVTPGRPIERRTIAVSGHLDITTTEDTPF
                     RIIVVDLAGKELQVLNQNNTIEKNVKHSFRFALTVQGWQKGKYKILIQKEADKSTIKA
                     FDFEV"
     gene            complement(3330..4901)
                     /locus_tag="PKN22_04740"
     CDS             complement(3330..4901)
                     /locus_tag="PKN22_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42044.1"
                     /translation="MIKQLFTFAFIILGSLLAMGQKEQLEWGELKGNSGNLIEIMPLN
                     GKDFYTLRWKGGNAFGGYYLSRYTDLKEVKSKRVSIAVNYNIANFEDVIIVDEHPSVI
                     LTNIKDGKEQVYLQQYSYELEPRGEAKLLANYDIQKGMSKEDIKIIQSKDKEYFAVLW
                     LLIGRKKDHDVYGYAIYNKKFKLMDQGEYEIPFESRYSQLTHHLLSNTGDYFFVIKEF
                     EPNQTRGLNQPDLMYKNMHIFQVNDGELKNYTLPFQGKRVEAISVNTNDSSSYIITGV
                     YGANEFNGAQGVFYMKLDFKNQAITQQSFHEFDKDFITEDWTKREKERAERREARGVD
                     EPSLYNYQMREAQLLPDGSMIGIMEQNYVVVSTFSDVRSIMTISYTYYYNDIVLFKIN
                     KDGAFGWVTKVKKSQISTNDGGPFSSFASISDSSTIRLIFNDNIENYDEQGKYIPKSE
                     NYTRFSKRYNTVALVEVDVKSGEQKRNTLFSRKETKTIAVPKLFKVDMNSKEMLLYTM
                     LNGRELYGILNIGNQ"
     gene            complement(4898..>5780)
                     /gene="purB"
                     /locus_tag="PKN22_04745"
     CDS             complement(4898..>5780)
                     /gene="purB"
                     /locus_tag="PKN22_04745"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNS42045.1"
                     /translation="WKDIPMLARTHGQPASPTRLGKEIQVFEERIERQLDLLKQIPYS
                     AKFGGATGNFNAHFVAFPQTDWVKFANNFVNNGLGLNRSQTTTQIEHYDNLAALFDAL
                     KRINTIVLDLNRDMWTYISEEYFKQKLKEGEIGSSAMPHKVNPIDFENSEGNIGIANA
                     IFEHLAIKLPVSRLQRDLTDSTVLRTIGMPFAHTFIAFKSTLKGLDKLLLNEQAFHDD
                     LENNWAVVAEAIQTILRREGFEKPYEALKGLTRKNEQVTKQTVHAFVDTLNISDALKA
                     ELKQITPHNYTGIQLVP"
BASE COUNT         1708 a         1122 c          972 g         1978 t
ORIGIN      
        1 caccaacttg tgaaccgttg tagttgattg tttctttccc ggtcaatacc aaatcaaatc
       61 ctttgtcttt tgcatattct gcaatttgca tcgctgtgta atatgcatct tttggatcag
      121 catcaattct tactgcatca tcagcaccaa tagccaacgc tttgcgaatt accgcctcgt
      181 ctgcagcagc tccaactgta acaattgtaa ctgaaccacc caacgtctct tttaattcca
      241 acgcacgaac caaagcatac cactcatcgt aaggattcac aatatactga acaccattct
      301 catcaaactt tgtattgcca tctgtaaaag caatttttga agttgtgtct ggtgatttcg
      361 aaatacctac taatattttc ataaatctat tattttttca attttcgtgt gcaaatctaa
      421 ttaaaaacaa tttaaaaagc tatacatttt gaaggagttt attaacaatt tagctatgca
      481 cgcatatatt ttaacaaatc agatggtttt atcctcgttt tccaacgggc tttgtttttg
      541 tttacaacct caaaaaatga taaaaattgt tattttagca acttgaaaca gaaattttca
      601 atctttctat taaatttgac cccatgagcg agaaaaaaaa taacgataac atcgacaaag
      661 cgaattttca ctttggattt agaaaagaaa actataaatt gctttttatc ggtttggcta
      721 ttaatatttt aggatatatt ttaatgattg gtggtggttc taaagatcca aatgtgttta
      781 ctggcgacca attattcaat actacacgtt taacaatagc tccaattctt atcatcgctg
      841 gttttgttat catgatttat tcaatcatga agaaaagcaa atcaagcaac tcaacttctg
      901 aagaataatt gcaatgaatt ttattgaagc catcattctt gccatcattg aaggcttgac
      961 tgagttttta cctgtttcat caacgggaca catgattatc ggttcatcat taatgggaat
     1021 tgccgatcaa gattttacca aattgtttac catcgttatt caattgggcg ccattctttc
     1081 ggtggtggtt atgtatttta aacgcttttt tcaatccata aacttctaca tcaaattatt
     1141 ggttgctttt attccagcgg cggtttttgg tgttttgctg agcgataaaa ttgatgaatt
     1201 attggaaagt ccaacaacag ttgccatttc acttgttatt ggtggtatta tcctactttt
     1261 tgtggacgat tggtttaaaa atcctacagt caacgaagaa aaagaaatta gttatttaac
     1321 agcattgaaa attggtttct tccaatgttt ggctatggta ccaggtgtat ctcgttctgc
     1381 cgcaacgatt gttggtggaa tgtcgcaaaa actttcacgt agtgcagctg ctgaattttc
     1441 atttttccta gctgtgccta caatgttggg tgcaacttca aaaaaattat tggattattt
     1501 tcaagatggc aactcgttta cttcagaaca aatcaacttg cttattgtgg gaaacatcgt
     1561 agcgttcatc gttgcattta tcgccatcaa aggatttatc agctttgtgt caaaacgcgg
     1621 atttaaagca tttggaattt atagaatagt agtaggttta ttaattctta tcttgatttg
     1681 gtcggggttt gacttaaaag tagtgtaatg gcatacgatt ttagcgcagg ggaaattctt
     1741 ttaattgaca aaccttacaa atggacttct tttgacgtgg tcaacaagct ccgttggaac
     1801 ttacgcaaaa aatacggcat caaaaagttt aaagtggggc acgcaggtac gctcgaccca
     1861 ttggcaactg gcttattggt gatttgcaca ggaaaagcaa ctaaaaaaat tccagattta
     1921 accaacgacg ataaagccta taccggaacg ttcttaattg gaaaaactac accctgtttt
     1981 gacctcgaaa aaccttttga cgacaaaata tatccgacag aacacatcac cgatgaattg
     2041 atgcaatcag ttgttcaatc gtttttaggt gtgcaagatc aatatcctcc tattttctct
     2101 gctaagcaaa ttgacggcaa aagagcttat gatttggcac gtgctggcaa agaagtggaa
     2161 atgaaaccca accaaattga aattaaagaa tttaccattt ctaccgaacg ttttccagaa
     2221 gtagattttt atgttaaatg ctccaaaggc acctatattc gttccattgc caacgatttt
     2281 ggtgtaaaac ttaactcagg agctacgctc attgcgctca accgcgtaca atcgggtgat
     2341 tttttactgg aaaacgcaaa gagagtagat gaatggattg cattaattaa cgaggaaaat
     2401 taaacctcaa aatcaaatgc ctttatggtg gatttatccg cttctttttg aataagaatt
     2461 ttgtattttc ctttttgcca accttgcacg gttaaagcaa aacgaaacga gtgttttaca
     2521 tttttttcaa tggtattgtt ttgattcaac acttgcaatt cttttccagc caaatccaca
     2581 acaataattc gaaacggcgt atcttcggtt gtggtgatgt ctaaatgtcc agaaacggca
     2641 atagttctgc gctctatcgg tcgcccaggt gttaccccca ccgattgttg cttgtaatac
     2701 cacggattag tacgattggt ttttttagca ataaactcac gcaacttggt gtccgcatcg
     2761 ttttttacct caatggattc aataggctct ttatctgtca acgcccaaac cgcttcttgg
     2821 taatttgatt tggaaggctg attggttttt aaaaactcta aaaaagccaa caacagcgta
     2881 tttttggttt tcccgattgt gaatttactg tcgagacgtg gcgcaatgcg tttcaattga
     2941 gtgcaatatc cgccaactct cacctttttt tgctcgtttt gttgcaaaac aaacaattgt
     3001 tcttcaatat tcaacaattc ttgttcttca tcgcttgatt taaacaacgt tccagctgga
     3061 acgacaattg taattggact tgcttgcaaa ttggtgaggg ttaaaatcac gccgttgttg
     3121 cccaattttt cataactggt taaaactttt atttttcctg ttgcaattgc ttctgtaata
     3181 tcaatcggtg ctgcccatcc tatgtatccc cataagatta gagcaataaa aataaggtgt
     3241 ttcatggctt ctagttttct tgtataacgc catcattttc aaaaaattac ataattggaa
     3301 taaaatacgt gaaacttcgt atttaactat tattgattgc caatattcaa aataccatac
     3361 aactcacgac cgttcaacat ggtgtagagc aacatttctt ttgaattcat atctactttg
     3421 aacaattttg gaacggcaat cgtttttgtt tctttgcgag agaacaacgt gtttcttttc
     3481 tgttcacccg attttacatc cacttccacc aatgccaccg tgttgtaacg tttactgaaa
     3541 cgagtgtaat tttctgattt tggaatgtat tttccttgtt cgtcgtaatt ctcaatatta
     3601 tcgttgaaaa ttaaacgaat ggttgatgaa tcggaaatac ttgcaaacga gctaaatggt
     3661 ccgccatcat tagtagaaat ttgcgatttt ttgactttcg ttacccaacc aaacgctccg
     3721 tctttattga ttttaaacaa aacaatatcg ttgtaataat aggtataact aatggtcata
     3781 atgcttctaa catccgaaaa agtactcact accacatagt tttgctccat tatgccaatc
     3841 atacttccat cggggaggag ttgtgcttcg cgcatttggt agttgtacaa agaaggttca
     3901 tccacccctc ttgcctcgcg tctttcagca cgttcttttt cgcgtttggt ccaatcttcg
     3961 gttataaaat ctttatcaaa ttcatgaaaa ctttgttgag ttatcgcctg atttttaaaa
     4021 tccaatttca tgtaaaaaac cccttgagcg ccattaaatt cattggcccc ataaactcca
     4081 gtaatgatgt aggaactgct atcgttggta ttgacagaaa tagcttctac cctctttcct
     4141 tgaaacggaa gtgtgtaatt ttttaattca ccatcgttga cctgaaaaat atgcatgttt
     4201 ttgtacatca aatcaggttg attcaatcct cttgtttgat taggttcaaa ttctttgatt
     4261 acaaaaaagt aatcgcctgt attgcttagc aaatggtgtg tcagttgtga gtaacgtgat
     4321 tcaaaaggaa tttcatactc accttgatcc atcaacttaa actttttgtt gtaaatggca
     4381 taaccataca catcgtggtc tttttttctg ccaatgagca accacaaaac cgcaaaatat
     4441 tctttgtctt tggattggat aatttttata tcctctttgg acattccttt ttgaatatcg
     4501 tagttggcaa gcaattttgc ctctcctcga ggttctaact catagctgta ttgctgcaaa
     4561 taaacttgtt ctttaccgtc tttgatatta gttaaaatca ccgatggatg ctcgtccaca
     4621 ataattacat cttcaaaatt ggcaatgttg taattaaccg caatagaaac acgttttgat
     4681 ttaacttctt tcaaatcggt atatcgtgat aagtaataac caccaaaagc attgccacct
     4741 ttccaacgca aggtgtaaaa atctttacca ttaagtggca tgatttcaat taaattacca
     4801 ctatttcctt tcaactcgcc ccattccaat tgttctttct gacccattgc tagcaacgaa
     4861 ccaagaataa taaaggcgaa tgtgaaaagt tgttttatca tggcactaat tgaattccgg
     4921 tataattatg cggtgttatt tgctttaatt cagctttcaa cgcatcgctg atgtttaatg
     4981 tatcaacgaa agcatgtaca gtttgttttg taacctgctc attttttctt gtcaaccctt
     5041 ttaatgcttc ataaggtttt tcaaatcctt cgcggcgtaa aattgtttga attgcctctg
     5101 caacaaccgc ccaattgttt tccaaatcgt cgtgaaaagc ttgttcgttc aacaacaatt
     5161 tatccaatcc tttcaacgta gatttaaatg caatgaaggt atgtgcaaac ggcattccga
     5221 tggttctcaa aacagtagaa tcggttaaat cgcgttgcaa tcttgaaaca ggcaatttga
     5281 ttgctaaatg ttcaaaaata gcgttggcaa taccaatatt cccttctgaa ttttcaaaat
     5341 caataggatt taccttgtgc ggcatggcag atgaaccaat ttctccttct ttcaattttt
     5401 gtttgaaata ttcttccgaa atataagtcc acatgtctct gttcaaatcc aacacaatgg
     5461 tattgatgcg tttcaaagca tcgaaaagtg ctgctaaatt gtcgtaatgt tcaatttgtg
     5521 tggttgtttg cgaacggttt aaccccaatc cattgttgac aaaattattc gcaaatttca
     5581 cccaatcggt ttgaggaaaa gctacaaaat gtgcattaaa attccctgtt gcacctccaa
     5641 atttagccga atatggaatt tgcttcaata aatccaactg acgttcaata cgttcttcaa
     5701 acacttgaat ttcttttcct aaacgtgtag gcgaagctgg ttgtccgtgt gttcttgcca
     5761 acattggaat gtctttccac
//
LOCUS       DAOQVQ010000111         5774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15332_length_5774_cov_4.245847, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000111 DAOQVQ010000000
VERSION     DAOQVQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15332_length_5774_cov_4.245847"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKN22_04750"
     CDS             <1..211
                     /locus_tag="PKN22_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNS42046.1"
                     /translation="YYFISTLISILVMVAIKQFFVDEDKLKAKMAEKKAAANSGNGPK
                     KKSKFQERLEMMQKMQQEQMKNKKK"
     gene            complement(339..1970)
                     /locus_tag="PKN22_04755"
     CDS             complement(339..1970)
                     /locus_tag="PKN22_04755"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNS42047.1"
                     /translation="MSRFIGLLAILAILLSSCMKAHHVDLVVHNAVIHSLDETMTIYE
                     AMAIKDGKIVELGPERQILNKYSSDEAYDAGGKNIYPGLTDAHVHLLYAAKQRMGVDL
                     SGSNSFAQLLMDVEMYQQRRPQKFIVGFGWNESLWRDNSMPTNAKLNELFPSTPVCLF
                     RNDGHTALVNEALLKLANITTETTVADGEIHKIDGQLTGILTDGAMHLVQEKLPKFSL
                     NELKEKVIEIQNELLMYGITNVHDAGLEADEVKMYQDLIDKKRFKLNFYGMLLPTEEN
                     IQFAKKNKVFEYKNMTIRSFKVFVDGSLGSWGAYLKQPYSDKVTTSGYPTITKEKLDE
                     LVQFCSSIGYQLNAHAIGDAAVKMVLDAYQPIAKLNPDHRWRIEHCQVVDPTDLKLFL
                     KLGVIPSVQPTQALSDYSFAESRLGKERLKTAYAYKSLLTQTGILLLGTDFPIESMDP
                     FKTLYAACVRKNDKDLPELGFLPSEALELDDFLKGSTIWPAIGSFQEQRNGSLEVGKE
                     ATFAVFESKINVPQNFVNNFSLLTYIKGEKVYSAQ"
     gene            complement(2001..2738)
                     /locus_tag="PKN22_04760"
     CDS             complement(2001..2738)
                     /locus_tag="PKN22_04760"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNS42048.1"
                     /translation="MNQIGCKIIHLTSVDSTSNYVANLQKEGVLAHGTAIIADEQTQG
                     RGQRGTVWNTTPGLNLTMSFYVEYTDLSIENQTSIHHWVAISVVEFLRKLGVETCIKW
                     PNDILAGSGKMAGILIENTLAGKFIKSSIIGIGLNVNQTDFGDVNGTSIKLETGNNFP
                     VAEIAQKLLLELDQQYSRLTYKAFKALKTDYLKNLWRMNQEVRFIRNNQLERGTILGV
                     NDGGLLEMQTQHGVEYFDLKEVKFMLD"
     gene            2813..3187
                     /gene="rsfS"
                     /locus_tag="PKN22_04765"
     CDS             2813..3187
                     /gene="rsfS"
                     /locus_tag="PKN22_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNS42049.1"
                     /translation="MQKEKKISSELLCDTIVEGMQENKAKNITVMDLRKIPGAICDFF
                     IICSGESSTQVDGISNAIQRLTRKELQEKPWHVEGKNGSEWILLDYVNVVAHVFYKEL
                     RGFYDLEDLWADAKITQIPDLH"
     gene            3204..5318
                     /gene="ftsH"
                     /locus_tag="PKN22_04770"
     CDS             3204..5318
                     /gene="ftsH"
                     /locus_tag="PKN22_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNS42050.1"
                     /translation="MQKNKNSNKKTPFSIYWVYAILGIGLIAYQYYYSTNSKIPLKTM
                     AQFETLADSGYVENVAIVINKLRVDIRLSKEGSEYLKETDNPQFKAITAYLKKNKDFR
                     NKTQEKENLELNISDNGNFEKKLEEINKELKAKNKQPISYTSETEYDYLMSILGFLLP
                     IILIFGLWMFIMRRMSGGGGGPGGQIFNIGKSRAQVFENGKSTNVTFKDVAGLQGAKE
                     EIQEIVEFLKNPKKYTELGAKIPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLSGSD
                     FVEMFVGVGASRVRDLFKQAKEKSPSIIFIDEIDAIGRARSKNGGFSSNDERENTLNQ
                     LLTEMDGFGTNSGVIILAATNRADILDKALMRAGRFDRQIYVDMPDINERKEIFGVHL
                     KPLKLAKDIDINFLSKQTPGFSGADIANLCNEAALIAARKEKKEVEKQDFLDAVDRIV
                     GGLEKKNKIITKDEKRSIAFHEAGHATISWMLEHAHPLVKVTIVPRGQSLGAAWYLPE
                     ERSITTTEQILDDMCSALGGRAAEQLIFGRISTGALSDLEKVTKQAYAMVSIYGLNDR
                     IGNISYYDSQGRESFQKPYSEETARIIDEEVSKLIESQYQRALQILSENKDKLTQLAE
                     KLLSAEVIFKEDLEEIFGKRPWDKEHVQEDLQKVEVPEELKAETVANESTEDTATVKS
                     SEQEVEQPSYEAKDEQKEEPIA"
     gene            5374..>5774
                     /locus_tag="PKN22_04775"
     CDS             5374..>5774
                     /locus_tag="PKN22_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HNS42051.1"
                     /translation="MRVFEGLEQVKEIKNPVLTIGTFDGVHLGHQKIIAYLNQKATEI
                     GGESVLFTFYPHPKMVIQPDNHGIKLLQTQEEKLDKLASTGLQNIIIQPFTTDFSQLS
                     AMEFVEKFLVNQLGVKHLVIGYDHHFGKGREG"
BASE COUNT         1929 a         1034 c         1069 g         1742 t
ORIGIN      
        1 ttattacttt atttcaacat tgatttccat tttagtaatg gtggctatca aacaattctt
       61 tgttgatgaa gataaattga aagctaaaat ggctgagaaa aaagcagctg caaattcagg
      121 aaacggtcca aaaaagaaat ctaagtttca agaacgtttg gaaatgatgc aaaaaatgca
      181 acaagaacaa atgaagaata aaaagaaata attgttcttc ttaaaaatac gaaaagagat
      241 tgtcaaacgg caatctcttt tttgtttata gttggtctgt ttggaattta attcaaaaat
      301 ctctatcctt taatttgaat ggagttgctc aattattatc actgtgccga atacaccttc
      361 tcacctttga tataggtcaa caaagaaaaa ttattgacaa aattttgagg aacatttatc
      421 ttgctttcaa atacagcaaa cgttgcttct ttgccaactt ctaagctacc atttcgttgc
      481 tcttgaaaac ttccgatagc cggccaaatg gttgaacctt ttaaaaaatc atctaattcc
      541 aatgcttctg agggtaaaaa ccctagctct ggaaggtctt tatcattttt gcgtacacaa
      601 gcagcataca aggttttaaa tgggtccatg gactcaattg gaaaatcggt acccaacaac
      661 aatatacctg tttgagtcaa caaagattta taggcataag ccgttttcaa acgttctttt
      721 cctaacctcg attcagcaaa agaataatcg cttagtgctt gcgttggttg aaccgaagga
      781 ataacaccta gttttaaaaa caacttcaaa tctgttgggt caacaacctg gcaatgttca
      841 atgcgccaac ggtgatcggg atttaatttg gctatcggtt ggtaggcatc caacaccatt
      901 ttcactgctg catcgccaat ggcatgtgcg tttaattgat atccaattga cgaacaaaac
      961 tgcaccaatt catccaattt ttcttttgtt attgttggat agccacttgt tgttacttta
     1021 tctgaataag gttgtttcag ataggcgccc caactaccga gtgaaccatc cacaaaaact
     1081 ttaaagcttc ggatggtcat gtttttgtat tcaaacacct tatttttctt tgcaaattga
     1141 atgttttctt cggttggcaa caacattcca taaaaattca acttgaatcg ttttttatca
     1201 atcaaatctt gatacatttt cacttcatcg gcttccaatc ccgcatcgtg cacgttggta
     1261 atgccataca tcaacaattc gttttgaatt tcaatgactt tttctttcaa ttcgttcaat
     1321 gaaaattttg gcaatttttc ttgtaccaaa tgcatggcac cgtcggttaa aatacctgtt
     1381 aattgtccat caattttatg aatttcacca tcagcaactg ttgtttctgt agtaatatta
     1441 gccaatttca atagcgcctc attcaccaaa gccgtatgcc catcgttgcg aaacaaacat
     1501 acgggtgtag aaggaaaaag ctcgtttaat ttagcattcg ttggcataga attatcgcgc
     1561 cacaaacttt cattccaacc aaaaccaacg atgaattttt gaggacgacg ttgctgatac
     1621 atttctacat ccatcaacaa ttgagcaaat gaattacttc cagacaaatc cacccccatg
     1681 cgttgtttag cagcatataa aagatgaaca tgagcgtcgg ttaatcctgg ataaatgttt
     1741 ttaccacctg catcatacgc ctcgtccgaa gaatatttgt taagaatttg acgttctgga
     1801 cccaattcca caatttttcc atctttgata gccattgctt cgtagatggt catggtttca
     1861 tccaacgaat gaataacggc attgtgcacc accaaatcca cgtgatgtgc tttcatacat
     1921 gaagacaaaa gaattgctaa aatcgccaga aggccaataa atcgagacat attttttatt
     1981 tgcaaagtta agaattaagg tcaatcaagc atgaatttca cttctttcaa atcaaaatat
     2041 tcaactccat gttgtgtttg catttccaac aaaccgccat cattgacacc taaaattgtt
     2101 cctctttcaa gttgattgtt tcgaataaaa cgcacttctt ggttcattct ccataaattc
     2161 ttcaagtaat ctgtttttag tgctttaaac gctttgtagg tcagacggct gtattgttga
     2221 tccagctcaa gcaatagttt ctgagctatt tctgcaactg gaaaattatt acctgtttct
     2281 agttttatgc tcgtaccatt cacatcgcca aaatctgttt gattgacatt taaaccaata
     2341 ccaataatag acgatttaat gaatttaccc gccaatgtgt tttcaattaa aatacctgcc
     2401 attttgccac ttcctgcaag aatgtcattt ggccatttta tacaagtttc cacgcccaat
     2461 tttcgtagaa attcaacaac agaaattgct acccaatgat ggatacttgt ttgattttca
     2521 atcgataaat cagtatattc tacataaaat gacatggtta gattaagccc gggagtggtg
     2581 ttccaaacgg tacctcgttg tccacgtccc tgcgtttgct cgtctgcaat tattgcagtt
     2641 ccatgtgcca aaacaccctc tttttggaga ttggcaacat agttgctagt agaatccacg
     2701 ctagtcaggt ggataatttt acatccaatt tgattcatta attttatttc tttgacgaat
     2761 atatgaagta aaattaaaaa tagcattaga tttgtaaata cgaaagaaat taatgcaaaa
     2821 agaaaagaaa atcagttccg aattattgtg tgatacaata gttgaaggga tgcaagagaa
     2881 caaggcaaaa aacatcaccg taatggactt gcgtaaaatt ccaggcgcca tttgtgattt
     2941 cttcatcatt tgtagtggtg aatctagtac acaagtggat ggaattagca atgccattca
     3001 gcgtttaaca cgaaaagaat tgcaagaaaa accttggcat gtggaaggca aaaacggtag
     3061 tgaatggatt ttgctagatt atgtaaatgt tgtagcccat gtattctaca aagaattgag
     3121 aggattttat gacttagaag atttatgggc agatgctaaa attacgcaaa tccctgactt
     3181 acactaaaaa aaacaacaac aagatgcaaa aaaacaagaa ttcaaataag aaaacacctt
     3241 tttcaatata ttgggtgtat gccattttag gtattggatt aattgcttac caatactact
     3301 acagcaccaa cagtaaaatt ccattgaaaa caatggccca atttgaaaca ttagccgaca
     3361 gtggttatgt tgaaaatgtt gcaatcgtta tcaataaact tcgtgttgat atccgcttga
     3421 gtaaagaagg atctgagtac ttaaaagaaa ctgacaaccc tcagtttaaa gctataacag
     3481 cctatctaaa gaaaaacaaa gactttagaa ataaaacaca agaaaaagaa aatttggagt
     3541 taaacatttc tgacaatggc aattttgaaa agaaattgga agaaataaac aaagaattga
     3601 aggctaaaaa caagcaaccg atatcctaca cttctgaaac agaatacgat tatttgatga
     3661 gtatccttgg tttcttattg cctattatcc taatttttgg attgtggatg tttatcatgc
     3721 gtagaatgtc tggtggcggt ggtggacctg gtggtcaaat tttcaacatt ggaaaatcgc
     3781 gtgctcaggt atttgagaac ggcaaatcaa ccaatgttac atttaaagat gtggctggat
     3841 tgcaaggtgc taaagaagaa attcaagaaa ttgttgaatt ccttaaaaac cctaagaaat
     3901 atactgaatt aggtgctaaa attccaaaag gagctttact tgtaggccct ccgggaacag
     3961 gtaaaacctt attagcaaaa gctgttgcag gtgaagcaaa agtgcctttc ttctcactat
     4021 ctggttcaga tttcgttgaa atgtttgtgg gtgtaggtgc atcacgtgtg cgcgatttat
     4081 tcaagcaagc taaagaaaaa tcaccctcta tcattttcat tgacgaaatt gatgcaattg
     4141 gacgtgccag aagtaaaaac ggtggattta gctcaaacga tgaacgcgaa aacaccttga
     4201 accaattgtt gacagaaatg gatggttttg gtacgaactc gggtgttatc atcttggccg
     4261 caaccaaccg cgccgatatt ttggacaaag cgttgatgcg tgctggtcgt tttgaccgtc
     4321 aaatttatgt ggatatgccc gatattaacg aacgcaagga aatttttgga gttcacttga
     4381 aaccgttaaa attggcaaaa gatattgata ttaatttctt atccaagcaa acaccaggtt
     4441 tctctggagc agatattgct aatttgtgta acgaagcggc gttaattgct gctagaaaag
     4501 agaaaaaaga agttgaaaaa caagatttct tagacgcagt tgaccgtatt gttggtggat
     4561 tggaaaagaa aaacaaaatc attacaaaag atgaaaaacg ttctattgcc ttccacgaag
     4621 ctggacacgc aacgatttct tggatgttgg aacacgcaca tcctttggtg aaagtgacaa
     4681 ttgttcctcg tgggcaatca cttggcgcag catggtattt accagaagaa agaagcatta
     4741 ctaccaccga gcaaatttta gatgatatgt gttcggcatt gggtggtaga gctgcagaac
     4801 aattaatttt tggaagaatt tcaactggtg cgttaagcga tttggaaaaa gttaccaaac
     4861 aagcctacgc catggtttct atttatggtt tgaacgatag aattggtaat atttcttact
     4921 acgattcaca aggtagagag tcgttccaaa aaccttactc tgaagaaaca gctcgtatta
     4981 ttgatgagga agtaagcaag cttattgaat cacaatacca acgtgctttg caaatcttgt
     5041 ctgaaaataa agacaagttg acacaattgg cagagaaatt gctttctgca gaagtgattt
     5101 ttaaagaaga tttggaagaa atttttggta aacgtccgtg ggataaagaa cacgtgcaag
     5161 aagatttgca aaaagttgaa gttccagaag aattaaaagc tgaaacggtt gccaatgaat
     5221 caacggagga tacagcaact gtaaaatctt ctgaacaaga agttgaacaa ccttcttatg
     5281 aagccaaaga cgaacaaaaa gaagaaccaa tagcataaaa agaaaggctg tctgttgatc
     5341 gggcagtttt ttttacaatt ttcgattcta ctcatgagag tttttgaagg tttagagcaa
     5401 gtaaaagaaa tcaaaaatcc ggtgcttacc atcggaacgt ttgatggtgt gcatttggga
     5461 catcaaaaaa taattgctta cttgaaccaa aaagctactg agattggagg tgaatcggtg
     5521 ttatttacat tttatccaca tcccaaaatg gttattcagc cggataatca tgggataaaa
     5581 ttacttcaaa ctcaagaaga aaagttagat aaacttgcct caacaggatt gcaaaacatt
     5641 atcattcaac cattcactac cgatttttcg caattatctg ctatggaatt tgtagaaaaa
     5701 ttcttggtaa atcaattggg tgttaaacat ttggtgatag gctacgacca tcattttggc
     5761 aaaggcagag aggg
//
LOCUS       DAOQVQ010000112         5677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15646_length_5677_cov_5.077019, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000112 DAOQVQ010000000
VERSION     DAOQVQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15646_length_5677_cov_5.077019"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PKN22_04780"
     CDS             <1..1051
                     /locus_tag="PKN22_04780"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNS42052.1"
                     /translation="IYSIVISLTFYIILNNKTMGNCDYCKSQDVSIYEASELSPFFKG
                     IIDLYDVDTENGKSLEVQIISDFHKKVFTQKLIETNNVKQLIADVIKDDVADYQNILD
                     NPVQLRFHNTSIENDVNQTLFLSWDRFSEEIKTVNRFHLKNPLDLEKLKSLFKYFQKD
                     LPKGKKFYRARISDNPKGYQIEQMGNPPHKSAKSGRANPNGISYLYLANDITTTLYEV
                     RASLFDFVTVGTFRLEESISVVNLSRSTYDVFRLAELESLESVMIHGSFIDKLEQELS
                     KPRRRSDSELDYLPTQYISELIKSMGFDGIEFKSSLYSEGINVAIFNPNKFKCLEVEV
                     YDITDIKLESEKLTN"
     gene            complement(1048..1215)
                     /locus_tag="PKN22_04785"
     CDS             complement(1048..1215)
                     /locus_tag="PKN22_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42053.1"
                     /translation="MRYFLSTVLLLLFLSCDFSTLTFAEKLDTLSEQQYKVVSVKDGD
                     TIGVLMDGKEN"
     gene            complement(1225..1845)
                     /gene="rsmG"
                     /locus_tag="PKN22_04790"
     CDS             complement(1225..1845)
                     /gene="rsmG"
                     /locus_tag="PKN22_04790"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNS42054.1"
                     /translation="MELIQKYFPELTSEQVQQFAQLKDLYQEWNEQINVISRKDIDNF
                     YERHVLHSLAIAKVMAFKDGSDIMDIGTGGGFPGIPLAILFPNSQFVLVDSIGKKVKV
                     ATEVAKALGLKNVTGIHERAEKIDQKFDFIVSRAVTAMPDFIRWTKNKFKPKGINDLA
                     NGILYLKGGDLAEEMKTVNKFYKFYNIPDFFSEEFFETKKVVYVRN"
     gene            complement(1864..2469)
                     /locus_tag="PKN22_04795"
     CDS             complement(1864..2469)
                     /locus_tag="PKN22_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS42055.1"
                     /translation="MVETDHLSDKGKQDLILVEAAIKGDQSAYAQLMSRYRDSVYFTM
                     QKMVKNVDDAEDLTIEAFGKAFSRLDQYSPSYAFSTWLFKIASNNCIDFIRKKRVQVT
                     SMSTGKVTEDGTVIYFDVRADNLNPEETIVQDQKIKLMRELVSKLKPRYKELVEKRYF
                     EELSYEEIAEEMNLPIGTVKAQLFRARDFLASMMDKSKDTI"
     gene            complement(2463..3593)
                     /locus_tag="PKN22_04800"
     CDS             complement(2463..3593)
                     /locus_tag="PKN22_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS42056.1"
                     /translation="MNIVPVFDFGWWSLLFYLFGAVVLIQLLYLIFFYARLAFYKEKP
                     NKEGALPPLSVIIAARNEADNIYENLPFILTQDYPEFEVVVVVNQTVDESKYILGAYQ
                     RQYPNLRFTVIEKSKHLRQGKKLPLNIGIKAAKYDHLVMTDADCKPVSKNWLREIGQS
                     FSDKKIVLGYGPHTKEKGFLNSVIRFDTAWIAVNYLSFALARLPYMGVGRNLAYTKDA
                     FQSVNGFKSHYNIASGDDDLFIQEAAKNRNYTISLAEESYMYSPSKTTWSSWYRQKSR
                     HYTTSPKYQVIKKALLGTYPMSLLILYISFVILLFNKDFRWITLIVFGVIFLLKWWIQ
                     GKCFLKLKEKSFVKLLPFNELAYAIGIPIIYYTSEVKTTNKW"
     gene            3671..4801
                     /gene="tgt"
                     /locus_tag="PKN22_04805"
     CDS             3671..4801
                     /gene="tgt"
                     /locus_tag="PKN22_04805"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNS42057.1"
                     /translation="MHFELIHTDSSTKARAGKLHTDHGVIETPIFMPVGTAGTVKGVH
                     QHELNDDIQAQIILGNTYHLYLRPGLDVIEAAGGLHKFNGWEKPILTDSGGYQVYSLS
                     GARKLSEEGARFQSHIDGRYLHFTPENVMDTQRTIGADIIMAFDECTPFPCDYNYAKK
                     SMHMTHRWLQRCFNRFNETEPKYGYQQALFPIVQGSVYKDLRTESAEVIASHNAVGNA
                     IGGLSVGEPDEDLYSMTDLVCSILPKDKPRYLMGVGTPQNLLECIALGIDMFDCVMPT
                     RNARHGLLFTWEGTINIKNQKWEKDFSPLDENGTSYVDRVYSKAYVRHLIKSNELLGA
                     QIASIHNLAFYLALVKEARKQILNGTFYSWKNQMVPKLERRL"
     gene            4810..>5677
                     /locus_tag="PKN22_04810"
     CDS             4810..>5677
                     /locus_tag="PKN22_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNS42058.1"
                     /translation="MLKILDKYIIKKFLTTFFFMLGIIMLLAMVFDLSDRLGEFIANE
                     APVSEIIFTYYLNFILFYGNSFSFMIIFISVIWFTAKMAQETEIIPILNSGKPFSRFI
                     RPYMIAATFLVIISLVINHIILPTFNKRALDFEDKYYRNALSVSNYFADFPGNITVQF
                     GNYTSEDNVAQQFVVQRFNKDKTLAYVLVARTAENIEGTNKWVLRDYYERTVGNPKDK
                     LREGQQLDTTFNFRMSEMAARDNIASAMRTGKLKKFIAREKEKGSSKVPLYELELYQR
                     TANPFATYILTII"
BASE COUNT         1813 a         1030 c         1021 g         1813 t
ORIGIN      
        1 gatctattca atcgtgatta gtttaacgtt ctatatcatt ttaaacaata aaacgatggg
       61 gaattgcgat tactgcaaat cgcaagatgt ttctatttat gaggcttctg aattaagtcc
      121 attttttaaa ggtattattg atttatacga tgttgataca gaaaatggga aatcattaga
      181 agttcaaata atttctgatt ttcataaaaa agtttttact caaaaattaa ttgaaacaaa
      241 taatgttaag cagctaattg ctgatgtaat caaagatgat gtagccgatt atcaaaatat
      301 tttagataat cctgttcagt tgagatttca taatacaagc attgagaatg atgtaaacca
      361 aactcttttt ctttcttggg atagattttc ggaagaaata aaaactgtaa acagatttca
      421 tttgaaaaac cctttggatt tagaaaaatt aaaatcattg tttaaatatt ttcaaaagga
      481 tttacccaaa ggtaagaagt tttacagagc aagaattagc gataatccca aaggttatca
      541 aatcgagcaa atgggtaatc ctccgcataa atcagcaaaa agtgggcgag ccaacccaaa
      601 tggaatttca tatctttatt tagcaaatga tattacgaca actttatatg aagttagagc
      661 aagtttattt gattttgtta ctgttggtac ttttagactt gaagaaagta taagtgttgt
      721 aaatcttagt cgttcaactt atgatgtgtt tagacttgct gaattggaat cattagaaag
      781 cgtaatgata cacggctctt ttattgataa attagaacaa gaattatcaa aaccgagaag
      841 aagaagcgat agtgaattag actatttacc aacacaatac atttctgaac ttataaaatc
      901 aatgggattt gatggtattg aatttaagag ttctctatat tctgaaggga ttaatgtcgc
      961 aatctttaat ccaaataaat tcaaatgtct tgaagtagaa gtttatgaca ttactgatat
     1021 aaaattggaa tctgaaaaat tgactaatta attttctttc ccatccatta aaacaccaat
     1081 agtatcgcca tcttttacac tcacaacttt gtattgttgc tctgatagcg tgtccaattt
     1141 ctccgcaaat gttagtgtgc taaaatcgca acttaaaaaa agcagcagta aaacggtact
     1201 taaaaaataa cgcattgcaa atgattaatt tcgaacgtaa actacttttt tggtttcaaa
     1261 aaattcttcg gaaaagaaat ctggaatgtt gtagaattta tagaatttat tgactgtttt
     1321 catttcttct gctaaatccc ctcctttcaa atacaaaata ccgttggcca aatcgttgat
     1381 gcctttgggt ttaaatttgt ttttggtcca acgaataaaa tcaggcatgg cagttactgc
     1441 tcggcttaca ataaaatcaa atttttggtc aattttttct gcacgctcat gaatgcctgt
     1501 aacatttttc aaccccaacg ctttagcaac ttcggtggca acttttactt ttttaccaat
     1561 agaatctacc aacacaaact ggctgttggg aaataaaatt gccaatggaa ttcctggaaa
     1621 accgccacct gtaccaatat ccatgatgtc gcttccatct ttaaacgcca ttactttggc
     1681 aattgcaagg gaatgcaaca catgtcgttc atagaaatta tcaatatctt ttcgagaaat
     1741 aacattgatt tgttcgttcc attcttggta taaatctttt aattgagcaa attgctgcac
     1801 ttgttcggag gtaagctccg gaaaatattt ttgaatcaat tccatggttg gaaaataaga
     1861 aaattaaatc gtgtctttag atttatccat catgcttgcc aagaaatcgc gtgctctaaa
     1921 caattgtgct ttcaccgttc cgatgggcaa gttcatttct tcggctattt cttcatagct
     1981 caattcttca aaatagcgtt tttcaaccaa ttctttgtaa cgtggtttga gcttggaaac
     2041 caactcgcgc atcaacttga ttttttggtc ttgaacaatg gtttcttctg gattgaggtt
     2101 gtcggcacgc acatcaaagt aaattacagt gccatcttcg gttactttac ccgtgctcat
     2161 agaagttact tgcactcttt ttttacggat aaaatcaatg caattgttgg aagcaatttt
     2221 aaacaaccac gtgctaaaag cgtaactggg tgaatattga tcgaggcgac taaacgcttt
     2281 tccaaatgct tctatggtta aatcttcggc atcgtcaacg ttttttacca ttttttgcat
     2341 agtaaaataa acagaatcgc ggtaacgtga cattaattga gcgtaagcag attggtcgcc
     2401 tttgatagcg gcctctacca aaattaaatc ctgttttcct ttgtcggata agtgatctgt
     2461 ttctaccatt tattggttgt ttttacctcg ctagtataat agatgattgg aattccaatg
     2521 gcataagcca actcgttgaa tggcagtaac ttcacaaatg atttttcttt gagttttaga
     2581 aaacatttgc cttgaatcca ccatttgagt aggaaaatta ctccaaaaac aatcaatgtt
     2641 atccagcgaa agtccttatt aaacaacaaa ataacaaaag aaatgtatag aattaacaac
     2701 gacatggggt aggttcctaa caatgctttt ttaataactt ggtatttagg tgaagtggtg
     2761 taatgacgtg atttttgtcg gtaccacgaa gaccatgttg ttttggacgg cgaatacatg
     2821 taactttctt cggctaaact gatggtgtaa tttctatttt ttgccgcttc ttgaataaac
     2881 aaatcgtcat ctcccgaagc aatattgtaa tgcgatttga acccattaac actttgaaaa
     2941 gcatctttgg tataagccaa atttcttccc acacccatgt aaggcaatcg cgccaaagca
     3001 aacgataaat aattgactgc aatccatgca gtatcaaaac gaataaccga atttaaaaac
     3061 cctttttctt tggtgtgcgg tccataaccc aacacaattt ttttatccga aaacgattga
     3121 cctatctcgc gcaaccaatt cttagagact ggtttgcaat cggcatcggt catcaccaaa
     3181 tggtcatatt ttgctgcttt aattccaata ttgagtggca attttttccc ttgacgcaaa
     3241 tgtttgcttt tttctatcac cgtaaaacgc aagttgggat attggcgttg ataggctcca
     3301 agaatgtatt tggattcatc caccgtttgg ttcaccacca ctacgacctc aaattcgggg
     3361 taatcttgcg ttaaaataaa aggcaaattt tcataaatat tgtctgcctc gttgcgcgca
     3421 gcaataatga cgctcaacgg tggcaacgct ccctccttat ttggtttttc tttgtaaaaa
     3481 gccaaacgcg cataaaagaa aataaggtaa agtagttgta tcaataccac agcaccaaag
     3541 aggtaaaaca acagcgacca ccaaccaaaa tcaaaaactg gaacaatatt cattcatcaa
     3601 actttagtgc aaatatgcta cgcttaatga aatagcttta tctttacact cgttattttt
     3661 caacaatgtt atgcactttg aattaattca taccgattct agcacaaaag cacgagctgg
     3721 aaagctccac acagaccacg gagtgattga aactccgata tttatgcccg ttggaactgc
     3781 cggcaccgtt aaaggtgttc atcagcatga attgaacgat gatattcaag cgcaaatcat
     3841 tttgggaaat acctatcacc tgtaccttcg ccccggattg gatgttattg aagctgctgg
     3901 tggattgcat aaatttaatg gttgggaaaa acctatttta accgatagtg gtggttatca
     3961 agtatattct ctttctggtg ctcgcaaatt gtcggaagaa ggcgctcgtt ttcaatcgca
     4021 cattgatggt cgttacttgc attttacacc tgaaaatgta atggataccc aacgcacaat
     4081 tggagccgac atcatcatgg cttttgatga atgcacccct tttccttgcg attacaatta
     4141 tgctaaaaaa tccatgcaca tgacccaccg ttggttgcaa agatgtttta accgtttcaa
     4201 cgagacagaa ccaaaatacg gttaccaaca agcgttgttt ccaattgttc aaggaagtgt
     4261 gtacaaagat ttaagaacag aatctgccga ggtgattgct tcgcacaatg ctgttggaaa
     4321 tgccattggt ggactttcgg ttggagagcc tgatgaagat ttgtatagca tgaccgattt
     4381 ggtgtgctcc attttgccta aagacaaacc tcgttattta atgggagttg gaacgcctca
     4441 aaatttattg gaatgcatcg ctttgggaat tgacatgttt gattgtgtaa tgccaacacg
     4501 caatgcccgc cacgggttat tgtttacatg ggaaggaacc atcaacataa aaaatcaaaa
     4561 atgggaaaaa gatttttcac ctttagatga aaacggaaca tcttatgttg accgtgttta
     4621 ttccaaagcc tatgtgcgcc atttgataaa atccaatgaa ttgttgggtg cacaaattgc
     4681 ttctattcac aacttggcat tttatttggc cttggtaaaa gaagctagaa aacaaatttt
     4741 aaacggtaca ttttattcgt ggaaaaacca aatggtgcct aaattagaac gtcgcttata
     4801 agaaaaaaca tgctgaaaat cctcgataaa tacatcatca agaagtttct tacaacattt
     4861 ttcttcatgt tgggaatcat catgctcttg gcaatggtgt ttgatttatc cgatcgattg
     4921 ggtgagttta ttgccaatga agctccagta agtgaaatta tttttacgta ttacttgaac
     4981 ttcattttgt tttatggcaa ctcgttttcg ttcatgatta tttttatttc cgtcatttgg
     5041 tttacagcaa aaatggctca ggaaacagaa attatcccga tactcaacag tggaaaacca
     5101 ttttcgcgtt ttattcgccc ttacatgatt gccgcaacgt ttttggtaat tattagtttg
     5161 gtaatcaacc acattatctt gccaacattc aataaaagag cgttggactt tgaagataaa
     5221 tactaccgga atgccttgag tgtttccaat tattttgctg attttccggg aaatataact
     5281 gttcagtttg gtaattatac gtccgaagac aatgtggcac aacagtttgt agttcaacgc
     5341 tttaacaaag ataaaacatt ggcctatgtg ttagtggcac gcacagcaga aaacatagag
     5401 ggaaccaaca aatgggtgct tagagattat tacgaacgca cggtgggtaa tccaaaagat
     5461 aaattgcgcg aagggcaaca attggataca acttttaatt tccgtatgag tgaaatggct
     5521 gcccgcgata acattgcatc agctatgcgc actggaaaat tgaaaaaatt catcgctcga
     5581 gaaaaagaaa aaggctcgtc aaaagtgccg ttgtatgaat tggaattgta tcaacgaaca
     5641 gccaatccat ttgctactta cattctcacc attattg
//
LOCUS       DAOQVQ010000113         5606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_15893_length_5606_cov_3.263196, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000113 DAOQVQ010000000
VERSION     DAOQVQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15893_length_5606_cov_3.263196"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKN22_04815"
     CDS             <1..312
                     /locus_tag="PKN22_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42059.1"
                     /translation="IVVLDGCADTSMCTTIEGLGLEDLVNGGKITLYPNPADNLVTIA
                     NLKSGSKLNVYDAAGKLVYFKEETSFEENINLTEFANGVYQVEITKDTSFEVLKLVVH
                     R"
     gene            424..2517
                     /gene="recG"
                     /locus_tag="PKN22_04820"
     CDS             424..2517
                     /gene="recG"
                     /locus_tag="PKN22_04820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNS42060.1"
                     /translation="MNTFLQTSIEFLKGVGPQRAELLKSELNIHTFEDLLNYFPFRYI
                     DRSQYYTIKELPYVDAAVQLKGRIIEIKESGVGKKRKLTAKFEDPTGTVELVWFQGIK
                     WVLPSLKKGVELQVYGKPKLFGTGWNIPHPEIVEYANVKNEVGWQAVYSTTEKLTAKG
                     LHSKGIEKLTKVLTEQIPQLQESLPEELVNRLNLLNRTQAYIQVHQPKNEELAHKARA
                     RFKFEELFYLQMELLVRKQISLKKSTGFVFEDVGELFNDFYKNHLPFDLTNAQKKVLK
                     EIRKDFLTGHHMNRLLQGDVGSGKTLVALLTTLIGIGNNYQAAIIAPTEILATQHFES
                     ISQLISNCGVKCALLTGSTKAKERKILHEELLSGDIQLLIGTHALLEDTVQFKNLGIV
                     VIDEQHRFGVAQRAKMRSKNTTPPHVLVMTATPIPRTLAMTFYGDLDVSVIDELPPGR
                     KPITTVHRTESSRSQVFGFMREEIAKGRQVYIVYPLINESETLDFNNLMDGFEAISRS
                     FPLPHYQVSIVHGKLNPEVKEYEMQRFVKGQTHIMVATTVIEVGVNVPNASVMIIESA
                     ERFGLSQLHQLRGRVGRGAEKSYCILMTGDKLSKDTKKRLDTMVRTNDGFEIAEVDLQ
                     LRGPGDIMGTQQSGELNLKIADLSRDTQLVVLARDEARNILNTDPNLENPENSIIRAI
                     LTQLLKSKPNWGKIA"
     gene            complement(2574..2972)
                     /locus_tag="PKN22_04825"
     CDS             complement(2574..2972)
                     /locus_tag="PKN22_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS42061.1"
                     /translation="MAIRRNKRFEIEIARDALSDIMFFLLLFFLIISTLANPNIIKMD
                     LPKADAPEKTSQKHLTLSVTKDEGYFIDKEPVAFENIESELMKKTAELGNNTVVVRIA
                     RDMEVQKLIDVLQIGTKNDLKFVIAADITQ"
     gene            complement(2953..3702)
                     /locus_tag="PKN22_04830"
     CDS             complement(2953..3702)
                     /locus_tag="PKN22_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS42062.1"
                     /translation="MKMKIKIKSIAFFAALFMIGIAFSQVETGGAAAVETTTHKVKEI
                     SALDFLMMGGVWIVPIILMLFYVISVGIEKTLYIRKVTKFDATLLNDMQNSLTQGNVE
                     NALATVSRDKTAYGAVIKEGVLTIGRPVSEIESNLDKATNIEIGKMEKNLSRIGMIAG
                     VAPTLGFIGTIAGVIKIFYSISVTENVSIGNISAGLYEKMIASGLAVGIIAFMTYHLL
                     NGAIDNFVLNAQKVNMDFINIIQRPNGDKKK"
     gene            complement(3887..4723)
                     /locus_tag="PKN22_04835"
     CDS             complement(3887..4723)
                     /locus_tag="PKN22_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNS42063.1"
                     /translation="MTRLFKYVFVIGFVANVFSSMAQNGEELFKNCFTCHTLGKKSTG
                     PDLVGVRQKWVDAGEGDLLVDWVKDANAVITGGKSKMANEIKDFSPLVMPPVKLTTEE
                     VNAIFDYIDSYKPAAPAAPAATASADATNPAAATAAPVEYKANYETNLVLFYLLIGLA
                     AIALVSIAMMAGSISTLTDSTYFKERVNKLLNGGGKTLTIALLMGMLLSGNSAMAMSF
                     GDSASNQPWLLVENSDIYLILFINIVLIGVVLYQRRLFSNIIGLVNNKKEVNASSEEE
                     SH"
     gene            5075..>5606
                     /locus_tag="PKN22_04840"
     CDS             5075..>5606
                     /locus_tag="PKN22_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS42064.1"
                     /translation="MIKIGVLGAGHLGKIHIRLIKELSDTYNLIGFYDPSDENAKAVE
                     EQFNIKRYPSIDELLNEVDCVDIVTPTIAHYTCATQAIRKSKHVFIEKPVTETVEEAK
                     SLMNLAQEAGVKIQVGHVERFNPAFQEAKKYLSNPMFIEAHRLSQFNPRGTDVPVVLD
                     LMIHDIDIILSVVKSGI"
BASE COUNT         1905 a         1036 c          906 g         1759 t
ORIGIN      
        1 atagttgtgc ttgatggctg tgcagatact tcaatgtgta caacaattga aggtttaggt
       61 ctggaagatt tggtaaatgg tgggaaaatc acactttacc ctaatccagc tgataattta
      121 gttactattg caaatctaaa aagtggatca aaacttaatg tttacgacgc cgctggtaaa
      181 ttggtatatt ttaaagaaga aacatctttt gaagaaaata tcaatttaac tgaatttgca
      241 aatggtgtgt accaagttga aataacaaaa gacacttctt ttgaagtttt aaaattagta
      301 gtacatcgat aacctcataa cttaaaacgc aaaatagggt cattgtagca atactttgac
      361 cctattttat ttttttacag atgaaatgct tctgtttctt tttttctttc ataaatttac
      421 cttgtgaata catttttgca aacatccatc gaatttttga aaggagttgg tccacaacga
      481 gctgaattgc ttaaaagtga actcaacatt cacacttttg aagatttatt gaactacttc
      541 ccttttcgct acatcgaccg ctcgcaatac tataccatca aagaattgcc atatgtggat
      601 gcagcggttc aacttaaagg tcgaattatt gaaataaaag aaagtggtgt aggtaaaaaa
      661 agaaaactaa cggccaaatt tgaagatcca actggaacag ttgagttagt atggtttcaa
      721 ggaattaaat gggtgctccc ttcacttaaa aaaggcgtag aactgcaagt atatgggaaa
      781 ccaaaattgt ttggcactgg atggaacatt cctcatcccg aaatagttga atacgcaaat
      841 gtaaaaaacg aggtgggttg gcaagcggta tattcaacaa ccgaaaaact aactgccaaa
      901 ggtttacact ctaaaggaat tgaaaaatta accaaagtgt tgacagaaca aattccgcaa
      961 ttgcaagagt cgttgcccga agaacttgtc aatcgattga atttattaaa cagaacccaa
     1021 gcatacatcc aagttcatca acctaaaaat gaggaattag cacacaaagc acgtgctcgc
     1081 ttcaaatttg aagaattgtt ttaccttcaa atggaattat tggtcagaaa gcaaatcagc
     1141 cttaaaaaat caacgggatt tgtgtttgaa gatgtaggtg aacttttcaa cgatttttat
     1201 aaaaaccact tgccttttga ccttaccaat gctcaaaaaa aagtgctcaa agaaattcgc
     1261 aaagattttt taactggcca ccacatgaac cgtttgttgc aaggcgatgt tggttcgggt
     1321 aaaacattag ttgcactact cacaacactt attggtattg gcaacaatta tcaagcagct
     1381 atcattgctc caaccgaaat tttggcaact caacattttg aatccataag tcaattaatt
     1441 agcaattgtg gggttaaatg tgcattgcta actggttcaa caaaagcaaa agaacgaaaa
     1501 attttgcatg aagaattgtt aagcggtgat attcaacttc taattggcac acatgccttg
     1561 cttgaagata ccgtgcaatt taaaaacttg ggaattgttg tcattgatga acaacaccgt
     1621 tttggagtgg ctcaacgtgc aaaaatgcgc tctaaaaaca caaccccacc tcatgttttg
     1681 gtaatgaccg caacaccaat ccctcgcaca cttgccatga cattttatgg cgatttggat
     1741 gtttctgtaa ttgatgagct tccaccagga cgaaaaccaa taacaacagt acaccgaaca
     1801 gaaagtagtc gctctcaagt ttttgggttc atgcgcgaag aaatagccaa aggaagacaa
     1861 gtatatatcg tttatccgtt gattaacgag tcagaaacat tggatttcaa taaccttatg
     1921 gacggatttg aagccatttc aagaagtttt cctctgccac actaccaagt gagcatagta
     1981 cacggcaaat tgaatcctga agttaaggaa tacgaaatgc aacgatttgt gaaaggtcaa
     2041 acacacatca tggtggcaac aaccgttatt gaagttggtg ttaatgtgcc caatgcatct
     2101 gttatgatta ttgaaagtgc agaacgtttt ggtttatcgc aattgcacca gttgcgaggt
     2161 cgtgttggac gcggtgctga aaaatcctat tgtattttaa tgactggcga taaattatct
     2221 aaagatacca aaaaacgatt agacaccatg gtgcgcacca atgatggatt tgaaattgcc
     2281 gaagttgact tgcaattgcg cggtccgggc gacattatgg gaacacagca aagtggtgaa
     2341 ctcaacctga aaattgccga tttatcgcgc gatacgcaat tagttgtttt ggctagagat
     2401 gaagcacgca acattctaaa caccgatccc aatttagaaa atccagaaaa tagcatcatt
     2461 cgagctattc ttacccaact tttaaaatca aaacccaatt ggggcaagat agcataaaaa
     2521 aagctgtcca atcattattg aacagctcat tatgtttcta gtttgttgtt gttttattgt
     2581 gtaatgtctg ctgctatcac aaatttcaaa tcgtttttag ttccaatttg caatacatca
     2641 attaactttt gaacttccat atctcgtgcg atgcgaacaa ccacagtatt atttcctaat
     2701 tctgctgttt ttttcatcaa ttcactttca atattttcaa aagctacagg ctccttatcg
     2761 ataaaatatc cttcgtcttt ggtaaccgac aatgtaaggt gtttttgaga tgttttttca
     2821 ggagcatctg cttttggcaa atccattttt ataatattag gatttgccaa ggttgaaata
     2881 atcaagaaga aaagcaataa gaagaacatg atgtcactta aagcatctct tgcaatttca
     2941 atctcaaaac gcttatttct tcttatcgcc attcggtcgt tgaataatat taataaaatc
     3001 catgtttact ttttgtgcgt ttaaaacaaa gttatcaatt gctccattca acaagtgata
     3061 ggtcataaac gcaataatac caaccgccaa accactggca atcatttttt cgtacaaacc
     3121 tgcagaaata ttaccaatac ttacattttc tgtaaccgaa atactataga aaattttaat
     3181 taccccagca attgttccga tgaatcccaa cgttggtgcc acaccggcaa tcataccaat
     3241 acggctcaag tttttctcca ttttaccaat ttcaatgttg gttgctttgt ctaaattaga
     3301 ttcaatttca gaaacagggc gcccaatagt caaaactcct tctttaatca cagctccgta
     3361 tgcagtttta tcacgagaaa cagtagctaa agcattctca acatttcctt gtgttaaact
     3421 gttttgcata tcgttcaata aagttgcatc aaacttggtt acttttcgga tgtataacgt
     3481 tttttcaatt cctacagaaa tcacataaaa taacatcaag atgattggaa caatccaaac
     3541 acctcccatc atcaaaaagt ctaatgctga tatttctttt actttatgag ttgttgtttc
     3601 aaccgctgct gccccacctg tttccacttg tgaaaaagca attccaatca taaataatgc
     3661 agcaaaaaat gcaatcgatt taatttttat tttcattttc attttcattc aattttcaac
     3721 gctaaaatta atggatatct ttctacaatt aagatatgac aaaagattgt tttaaacatg
     3781 aattgttaac aactgaaaaa cgttgtatat tttgttttat tttaaacact aaaaacaaaa
     3841 aaaggcgcag atgaaatcca cgccttaaaa acaacaactt acaaaattaa tggctttcct
     3901 cttctgaaga agcattaact tcttttttat tattaactaa accaattatg tttgaaaata
     3961 atcttctttg gtacaaaaca acaccaataa gtacaatgtt tatgaaaaga attaaataaa
     4021 tatctgaatt ttccaccaac aaccatggtt gattactagc agaatctcca aaagacatag
     4081 ccattgcgct attacctgac aacaacatcc ccattaacaa agcaatagtt aatgtctttc
     4141 ctcctccatt caacaattta ttaacacgct ctttgaagta agttgaatct gtcaacgttg
     4201 atatcgagcc cgccatcatt gcaattgata ccaatgcaat agctgctaga cctatcaata
     4261 aataaaagag aaccaagttg gtttcgtaat ttgccttgta ttctactggt gctgctgttg
     4321 ctgctgcagg atttgttgca tctgctgatg ctgttgctgc aggagccgca ggagctgctg
     4381 gtttatacga atcaatataa tcaaaaattg cgtttacttc ttctgtagta agttttactg
     4441 gtggcattac aagcggcgaa aaatctttga tttcatttgc cattttactt tttcctccag
     4501 tgattactgc attggcatct tttacccaat ccaccaacaa atcaccttca cctgcatcta
     4561 cccatttttg acgaacacca actaaatctg gacccgttga ttttttcccc aatgtgtgac
     4621 atgtgaaaca gtttttaaaa agctcttctc cattttgtgc cattgagcta aaaacattgg
     4681 caacaaatcc aattacaaat acatacttaa ataaacgtgt cataaaataa atgtttactt
     4741 tttacttaaa actattaact aaaaatgctt tacattcttg catttcaata gtgcaaaact
     4801 ataaatttta atgcaatttt aaataaaaaa tcgaatataa aatacataaa ttggatattt
     4861 ttatccgttt tttataaaca gctatgaatc aaattaacta aacacctgtt ttactgatta
     4921 ttacaaaaca cctgaaattc aacttttttt atattaaaaa tttatttgat actaaaatct
     4981 gaatatgact atttacgatg aaatgttgat aaaaaaaagc aaagcctaat tcttctaatg
     5041 cgctagtatt tatttttgca aaaacatctt ttcaatgatt aaaattggcg ttcttggtgc
     5101 aggtcacctt ggtaaaattc acatacgatt aataaaagaa ctttcggata cctataattt
     5161 gattggattt tatgatccat ccgacgaaaa tgcaaaagct gttgaagaac aattcaatat
     5221 taaaagatat ccgtctattg acgaattatt gaatgaagtg gattgtgttg atatcgtcac
     5281 tcctacaatt gcacactata cttgtgcaac acaagcaatt cgtaaatcta aacatgtatt
     5341 tattgaaaag ccagtaacag aaacagttga agaagctaaa tcattaatga atttagcaca
     5401 agaagcaggt gtaaaaattc aagtcggtca cgttgagcgc tttaaccctg catttcaaga
     5461 ggcaaaaaaa tacttgagca atcctatgtt tattgaagca catcggttat cacagttcaa
     5521 tccacgtggt acagatgtgc ctgttgtttt agacttaatg attcacgata ttgacataat
     5581 attaagtgtt gtgaaatctg gaatta
//
LOCUS       DAOQVQ010000114         5575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16022_length_5575_cov_3.445109, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000114 DAOQVQ010000000
VERSION     DAOQVQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16022_length_5575_cov_3.445109"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKN22_04845"
     CDS             complement(<1..677)
                     /locus_tag="PKN22_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42065.1"
                     /translation="MKYFHKYCLLFLFTQLLFFSFGQRKIVNAEYFIDTDPGVGLATP
                     ILAQDGSFDNAVEALFRNGISVASVGLHTLNLRVQDNNGNWGPVFKTVFNATSPLVTR
                     SIMVSQAEFFWDTDPGLGNGTTMIAFDGNFNDAIETISQVTGSLPTPGVHKLSIRVKD
                     VNNSWSSTFSTVLNITSPLTVRNIKISAGEFFWDTDPGMGSATPLLAFDGNFNEAIEA
                     LSLNTGSL"
     gene            complement(707..1993)
                     /locus_tag="PKN22_04850"
     CDS             complement(707..1993)
                     /locus_tag="PKN22_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42066.1"
                     /translation="MKNFSKKLALTLSVGALFSTQVIAADLCVNESGSGGCYASITDA
                     LTAAVDGDRIIIQPKSGGVPYVENITINKSVSLLSNTEGVQWLMTGNITITPAIGRTI
                     SILHMKNMSGNITASGHSPAGTRCKVNILNCELVSGYINFDYNYFNVNLASSEIKEGY
                     VNLRYGNVIGNVISASNYSNMTYISSNYILLYIGNDALATDDTLNIVGNRILNNSTNS
                     TYYMGVLINSSNHFFNISNNFITHPDTYYTSYGIYFQNHKNSMAGVNSVYNNSFYDSY
                     TSSYYYYAIAIGSNTAGRFDIKNNLSQSMRPAGYNAFSFTATPTIGFSFNFGTNAALS
                     GIPDDGTNNFASTSTINTTTGELNVGSDGFDAGYPDLSFYDLDLTRNDVGCYGGSFSQ
                     DNFFPITGAARVYFVKAPRTILQSGTLNVKAESFDR"
     gene            complement(2220..3416)
                     /gene="hflX"
                     /locus_tag="PKN22_04855"
     CDS             complement(2220..3416)
                     /gene="hflX"
                     /locus_tag="PKN22_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNS42067.1"
                     /translation="MGNGHVLVDAVEEKCILVGVITSEINEDIANEYLDELEFLAETA
                     GAITHARFLQKLPFPNPRTFVGTGKLEEIKSFVIENEIELCIFDDELSPSQLRNIERE
                     LQVKILDRNNLILDIFASRARTSHAKTQVELAQLQYMLPRLTRMWTHLERQKGGIGMR
                     GPGETQIETDRRIIGQKIALLKSRLSEIDKQMATQRSNRGQLVRVSLVGYTNVGKSTL
                     MNLLSKSDVFAENKLFATLDTTVRKVVIDNLPFLLTDTVGFIRKLPHQLVESFKSTLD
                     EVREADLLIHVLDIAHPNFEDHFKVVNETLNEIDKTEKPTIIVFNKIDQFVYVPKDED
                     DNSPIIAQNYTLDELKRTWMAKANTKCVFISAHEKTNIEELKTIIYEEVKRIFQELYP
                     YNNFLY"
     gene            complement(3416..3757)
                     /locus_tag="PKN22_04860"
     CDS             complement(3416..3757)
                     /locus_tag="PKN22_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNS42068.1"
                     /translation="MVLRFVTFLLVGITVVSCSSNSSESANKNETEQQVSAFDAVNLF
                     NTHCSTCHGLDGSLGVGGAKDLSKSAMDLTAIRKTIQSGTIGGMPRFKETFTTQEIDA
                     VAQYVITLKRK"
     gene            complement(3748..4167)
                     /locus_tag="PKN22_04865"
     CDS             complement(3748..4167)
                     /locus_tag="PKN22_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42069.1"
                     /translation="MLHAHSGLRWIALILLLLAIFNAFSKMKSPAVSAGDRKINLFAM
                     VTFHIQYLVGLGLYFTSPKVNFDQGWMKIDIFRFFGMEHVLMMTVAFILITMGFSKSK
                     KSATPYKTIAIFYTIALILILAGIPWPFRGALGGSWF"
     gene            4588..5316
                     /gene="ubiE"
                     /locus_tag="PKN22_04870"
     CDS             4588..5316
                     /gene="ubiE"
                     /locus_tag="PKN22_04870"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNS42070.1"
                     /translation="MGTPVKPYGSDDKSKKQEVAEMFNNISKKYDFLNHFLSVGIDKL
                     WRKKAVAQLKDIQPQRLLDLATGTGDFALALLKLNPKEIIGMDISEGMLEVGRKKMKE
                     KGVDNIISMQLGDSENLPFEDGYFDGLTVGFGVRNYENLEKGLCEMLRVLRVGGRAVI
                     LEFSKPKKFPVKQYYAFHSKYIIPFFGKLVSKDTSAYTYLPDSIQAFPEGKDFEAILA
                     KVGYKNIRSILVSGGIATIYIGEK"
BASE COUNT         1872 a          895 c          936 g         1872 t
ORIGIN      
        1 agtgaaccag tgtttaaact taaagcttcg atagcttcat tgaagtttcc atcaaaagct
       61 aataaaggag tagcgctacc cattccagga tcagtgtccc agaaaaattc acctgcagaa
      121 attttaatat ttcgtacggt aagaggggaa gtaatgttta acactgtgct aaaagtagaa
      181 ctccatgaat tgttaacatc ttttacccga atggatagtt tgtgaacacc aggggtaggt
      241 aaagagccag taacttgact aattgtttca atggcgtcat taaaattacc atcaaaagca
      301 atcattgttg taccgtttcc taacccagga tcagtatccc agaaaaactc tgcttggctt
      361 accatgatac tgcgagttac taatggggat gtagcattaa aaaccgtttt aaatactggt
      421 ccccaattgc cattgttgtc ttgaacacga agatttaagg tatgcaatcc tactgacgct
      481 acagatattc catttctaaa caatgcttca actgcattgt cgaaacttcc atcttgtgcc
      541 aaaataggag ttgcaagccc tacacctggg tccgtgtcaa taaaatactc agcatttact
      601 attttccttt gaccaaagct gaaaaataga agttgagtaa ataaaaacaa caagcaatat
      661 ttatgaaaat atttcatttt catattttta taaatgaggg ttcaaattaa cgatcaaaag
      721 actcagcttt tacgttaagt gttccacttt gtaaaatagt tcttggagct ttaacaaaat
      781 aaacacgtgc tgcaccagta attggaaaga agttatcttg gctaaaagaa cctccatagc
      841 aaccaacatc atttctagtt aaatctaaat catagaaaga caaatcaggg tagcctgcat
      901 caaagccatc agaaccaaca tttaattctc ctgtagtggt gttaattgtt gaagtactag
      961 caaagttgtt tgttccatca tcaggaatac cacttaaagc agcatttgtt ccaaagttaa
     1021 atgaaaatcc aattgtcgga gtggctgtga aagaaaaagc gttatagcct gctggtctca
     1081 ttgattgtga taaattattt tttatatcaa atctacctgc agtatttgaa ccaatcgcaa
     1141 ttgcataata gtagtatgaa ctagtatagg agtcatagaa tgaattgtta tatactgaat
     1201 ttacacctgc cattgagttt ttatggtttt gaaagtaaat accataagat gtataataag
     1261 tatctggatg tgttataaaa ttattagaga tattaaaaaa atgattggat gagttaatta
     1321 atactcccat ataataagtg ctattagttg agttatttaa gattctattt ccaacaatat
     1381 taagagtatc atcggtagca agggcatcat taccaatata caaaagaata taatttgaag
     1441 aaatataagt catatttgaa tagttagatg cagaaattac attaccaata acatttccat
     1501 aacgtaaatt aacgtaacct tccttaattt cactggaagc taaattgaca ttgaaatagt
     1561 tgtaatcaaa attaatgtac ccacttacta attcacaatt tagaatattt actttgcaac
     1621 gagtaccagc aggactatga ccacttgcag tgatatttcc actcatgttt ttcatgtgta
     1681 aaatactaat tgttctgcct attgctggcg taattgttat attaccagtc ataagccatt
     1741 gaacgccttc cgtattagac agcaaagaaa cagacttgtt gatagttata ttttcaacat
     1801 aaggtacacc accagatttt ggttggataa taattcgatc tccatcaact gcagccgtta
     1861 aagcatctgt tatagaagca tagcaacctc ccgatccaga ttcgtttaca cataaatcag
     1921 cagcgataac ttgagtgctg aataaagcac caacagacaa tgttaatgct agttttttcg
     1981 aaaaattttt catgatataa tgatttatta atttagaaat caaatgtata tgaaaaatat
     2041 ttaataaaaa aacaacaagg ataaagatat cttttttaaa gggggttaaa tttaattgaa
     2101 aataagtgaa tagccagata tatctaatgt ttacgtaata tttttcaatt ccataaaatt
     2161 actttaaaag aaaaaaatac gtagaattac gtagttggaa gaggtgttaa tttttattct
     2221 taatacaaga aattattata cggataaagt tcttggaaaa tgcgtttaac ttcttcgtag
     2281 atgattgttt tgagctcctc gatatttgtt ttttcgtgcg cagaaataaa cacacatttg
     2341 gtatttgctt ttgccatcca cgttcttttc aactcatcaa gggtgtagtt ttgagcaatt
     2401 attggactgt tatcgtcttc gtcttttggt acgtaaacaa attgatcaat tttattgaaa
     2461 acaataatgg taggtttttc tgttttatca atttcattca acgtttcgtt tacaaccttg
     2521 aaatgatctt caaaattagg atgtgcaata tcgagtacat gaattagcaa atcggcttcg
     2581 cgcacttcat ccaaagttga tttaaaagat tctaccaatt ggtgcggtag ttttctaatg
     2641 aaaccaacgg tatctgtaag taaaaacggc aaattgtcta taacaacttt tcgaacggtg
     2701 gtgtcaagag ttgcaaacaa tttgttttca gcaaacacat cagatttaga caacaagttc
     2761 attaaagtgc ttttaccaac gttggtgtaa cctactaatg aaacacgcac caattgccca
     2821 cgattgcttc gttgggttgc catttgtttg tcaatttccg ataatcgtga ttttaacaaa
     2881 gcaatttttt gaccaatgat tcttctatcg gtttcaattt gcgtttcacc aggtccgcgc
     2941 attccaattc cccctttctg acgttctaag tgggtccaca ttcttgttaa acgtggtaac
     3001 atgtattgca attgcgccaa ttcaacttgt gtttttgcat gtgaggtgcg tgcacgtgat
     3061 gcgaaaatat caagaattaa attgttgcga tccagaattt tcacttgcaa ttcgcgttca
     3121 atatttctca gttgcgaagg gcttaattca tcatcaaaaa tgcaaagttc aatttcattc
     3181 tcaataacaa atgattttat ttcttctagt tttccagttc caacaaacgt tctaggattt
     3241 gggaaaggca atttttgtaa aaaacgggca tgtgttatcg ctccagcagt ttctgctaaa
     3301 aattctaatt catccaagta ttcatttgca atatcttcat taatttcaga agttattaca
     3361 ccaacaagga tgcatttttc ttcaactgca tcaactaata catgtccgtt tcccattact
     3421 tgcgtttaag tgttataaca tattgtgcca cagcatcaat ttcttgggta gtaaaagttt
     3481 ctttaaatcg tggcattcca ccaattgtac ccgattgaat ggtttttcta attgccgtta
     3541 aatccattgc cgactttgac aaatctttag cgccacccac accgagcgat ccatccaatc
     3601 catgacaagt tgaacaatgc gtattaaaca aattaaccgc atcaaaagca ctaacttgct
     3661 gttctgtttc attcttgtta gcagattcgg atgaattgct agaacaagaa acaacagtta
     3721 ttccaactaa aaggaatgtt acaaatctta gaaccatgat cctcctaaag cacctctaaa
     3781 tggccaagga ataccagcca atattaaaat caatgcgata gtataaaaaa tagctattgt
     3841 tttgtatggt gtagctgatt ttttagattt tgaaaatccc attgtgatta aaataaaagc
     3901 aacggtcatc attaaaacat gctccattcc aaaaaaacgg aagatgtcaa ttttcatcca
     3961 accttggtca aaattcactt taggtgatgt aaaatacaat cccaatccaa ctaaatattg
     4021 aatgtggaaa gtcaccattg caaataagtt aatttttcta tcgccagcag aaacagcagg
     4081 agatttcatt ttgctaaaag cattaaagat tgctagcaat aaaagaatta aggcaatcca
     4141 tcttaaacca gagtgtgcgt gtaacataat tgttgttttt tatgcaaaaa tagtgaaata
     4201 atggaagtat ggagaactag ttgaattttt aggtggaata tttgttgata tcgaactatt
     4261 tcgatattgc gatatggttg agaaattaag tttataagtc aacacaattg tctgattttg
     4321 atttagttaa atgttaagat tgatatgtcg atatcgaact atttcgatat tgcgatatgg
     4381 ttgagaatta aagtttatga gtcaactcaa ttgttgaatt ttaattcagt tgaatgttga
     4441 ggttgatatg ttgatatcga actatttcga tattacgata tagttcaaaa tttcaacttt
     4501 atctctttca aaacttataa aacactattg actattaacc caatatttta gttatttttg
     4561 cagccaacat tataaaattc aatcaaagtg ggtactccag ttaagccata cggttctgac
     4621 gataagtcaa agaagcaaga agttgcagag atgtttaata acatttctaa aaaatacgat
     4681 tttttaaatc attttttatc ggttggaatt gacaagttat ggcgtaaaaa agctgttgct
     4741 caattaaaag atattcagcc acaacgtttg ttggacttgg caacaggtac tggtgatttt
     4801 gcattggcgt tattgaaatt aaacccgaaa gaaattattg gaatggatat ttctgaagga
     4861 atgttggagg ttggacgcaa aaaaatgaaa gaaaaaggtg ttgacaatat tatttccatg
     4921 caacttggag attctgaaaa cttacctttt gaagatggct attttgatgg tttgactgtt
     4981 ggttttggcg tgcgtaatta cgaaaatttg gagaaaggtt tatgtgagat gttgcgtgtg
     5041 ttaagggttg gaggaagagc tgttattctt gagttttcaa aacctaaaaa attccctgtt
     5101 aaacaatatt atgcttttca ttccaagtat attattccat tttttgggaa attagtttct
     5161 aaagatacaa gtgcctacac gtatttacca gattctattc aagcatttcc agaagggaaa
     5221 gattttgagg ctattttagc aaaagtaggt tataaaaata tccgttctat attagtttct
     5281 ggcggtattg ccaccattta tattggcgaa aaataatttc atgtaaaatt ttgctttagt
     5341 taggagtttg aaagggtatg tttttgcgta cccttttatt gcttttaaag actactaata
     5401 cattcctaaa tcatttgaat attctttgaa gtaaagttct ttagttggac tcgaatagtt
     5461 tattatttct actatacttt aattattttt tgattcaagt taattagaaa acttagtaag
     5521 tatgactagt cctaaataac cgatacagat tattaagaca aaaataatta attat
//
LOCUS       DAOQVQ010000115         5545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16141_length_5545_cov_3.171585, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000115 DAOQVQ010000000
VERSION     DAOQVQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16141_length_5545_cov_3.171585"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PKN22_04875"
     CDS             <1..975
                     /locus_tag="PKN22_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS42071.1"
                     /translation="QNRKLEQLDMFENKEIDLILELPTSKITKMIEGRLSDFNSTPPK
                     LLLFRNPSLNTHYINFNMKDPRFNDARVRKAFIYAFNKERMGREVLKNQFNELGYYGI
                     VPPVSSVFKGYDFEAIKKQGYEYNPELAKKLLAEAGYPNGKGFGSVNLRYSIGDINSA
                     VADEFSQQIFQVLGINVNIDGSSFEQLNEDAINGNGELFKSSWIADYPKPENFLTSFI
                     TSNIPTAENRSGMNYSKYSNPTYDKLIEEGNKESNIGKKLSLFNKAEIELLKNPPFMS
                     LWYNGDIEITYSYVRNLHFNSLSYFKFKKVYLKPWTAEEYQKEITNKK"
     gene            984..3437
                     /locus_tag="PKN22_04880"
     CDS             984..3437
                     /locus_tag="PKN22_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42072.1"
                     /translation="MLKLSSLLVFLLLAVLSFAQPVQNVRGRVFDSETFTPVVAAKVV
                     IPSKDTTKVYQALTDDKGEFLISNVVVGKYTLHVNFFGYDEKTVNLEVISGKESIIDV
                     PMTEAYVKNTDEVVVTARKSGEVINELALISARQFSVEETNRYAGSRSDPARMASNFA
                     GVQGADDSRNDIIVRGNSPLGVVWKVEGIDIPNPSHFATSGSTGGPVTILNNKILGNS
                     DFFMSAFPAEYGNSTAAVFDLKLRNCNNSNHEFTGQFGFLGTELLAEGPMSKTGNSSY
                     LIMGRYSTLSLFKMMGIKIGTDAVPEYGDGAFKFNWRFKNGGNLSFFGIGGKSNIQIM
                     ISEQKEFSSEMYGEGDRDQYFGTSMVVSGFNYKQSLSEKTFLTTTLAYSYDEQHSHHN
                     YLLRHLDTLNKGTADEAYQIAIDSIYPMMGYQFQFHKVAYHLAVTHKFNTRHLIKTGI
                     NTDLYYVEQKDSALRLVTDNQFQMRWDYKGAYTLIQPFIQWKWRATENMDVTAGLHAQ
                     YFSLSNRFSGVEPRLGWRWKIKNNHSVFAGAGMHSQTQPLYTYTYNKFDAAGNKIYHN
                     KNMDFSKSIHTGIGYEAFLPKSWNIKFEAYYQYLYNVPVTVTPSAFSLINMGAGFQRF
                     FPDTLKNSGTGRNYGFELTVQKFFDKSFFVLVTASVYDSQYKGSDGVMRNTSYNGNYV
                     FNVLGGKEFKIGKKQSISTGIKATYAGGQRYGYVNIPESEKYHELIYASDGYNSRRFK
                     DYFRLDLKLSWKYNARVLTHEIGLDLVNILNTKNVLSLTYAPNLADPTAEPLATKNQL
                     GFLPLFYYKIDFRFTKKKN"
     gene            complement(3434..4864)
                     /locus_tag="PKN22_04885"
     CDS             complement(3434..4864)
                     /locus_tag="PKN22_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS42073.1"
                     /translation="MQKKFISNLVLLVLLNLIVKPISIFGIDAAVQNRVGSADYGLYF
                     SLLNFTYLFNILLDFGINNYTTKNVAQYPALMKKYIGKIAGLRLVFFIVYAFITLGLA
                     LILQYDVSVYPILTVLIVQQFIIGMIAYFRSYLSGLLLFKLDALLSVLDRFLLIVLCG
                     SALYLHTVSNFTITTFIWLQFASYAISFLVGLVLVVKNAGKIKVSINKHISYLITRQS
                     LPYALLIILMMLFTRIDSVMIERMLPDGAKQAGIYAQGFRLLDAFFMFAMLFSNLLLP
                     LFSKMIATKENVQTLLLSSSKLLIWGSIALTALCFWNAFDLLDLIYNNDVSSTVLPFQ
                     LLMFTFISMCITLIFGTYLTALGNMRILNIIAGIGLVLNIAINLVLIPSYGVVGAAYA
                     TLITQSFVAFAEFVVVVKLLNLSFSLKSILVFPLYCIVVFGGLWLQKKYSLNIALQGI
                     FIGICLLLFKVVNVQFLRNLLSKNAD"
     gene            complement(4873..>5545)
                     /locus_tag="PKN22_04890"
     CDS             complement(4873..>5545)
                     /locus_tag="PKN22_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNS42074.1"
                     /translation="YLYIFSKFKINTLKNDANEKDFFHFLNLIQDKKGDLIDIGANIG
                     VMTYYLASKFPNLKVHSIEPIPVNFSVLKRIIARYQLKNVEVYPIALGQSKGKLKMVM
                     PYNGKTKMQGLAHVLHESIPSFNKGEEFEVPVKTLDELFLGDNIQAIKIDIENFEYFA
                     FLGGEQLLRDKKPIIYAELWDNDNRKNSFDLLKNIGYSVYVIENDKLVLFDEKKHITQ
                     NFIFK"
BASE COUNT         1968 a          948 c          968 g         1661 t
ORIGIN      
        1 caaaatagaa aattggagca attggatatg tttgaaaaca aagaaattga tttgatttta
       61 gaattgccaa caagcaaaat tactaaaatg attgaaggcc gtttgtctga tttcaattca
      121 actcctccaa aactattatt atttagaaac ccttcgctca atacacatta cattaatttc
      181 aatatgaaag atcctcgttt taatgacgcg cgtgttagaa aagcatttat ctatgctttt
      241 aataaagaac gaatggggcg agaagtattg aaaaatcagt tcaatgaatt gggttattat
      301 ggaattgttc ctcctgtttc ttctgtattt aaaggttacg attttgaagc aattaaaaaa
      361 caaggatacg aatacaatcc tgaattagct aaaaaactat tggcagaagc tggatatcct
      421 aatggcaaag gatttgggag tgttaattta agatacagta ttggcgatat taattcagca
      481 gttgctgatg aattctcaca acaaattttt caagtcttgg gcattaatgt caatattgac
      541 ggttcttctt ttgaacaact taatgaagat gcaatcaatg gaaatggtga gttgtttaaa
      601 agtagctgga ttgctgatta tccaaaacca gaaaatttcc tcactagttt tattacttcc
      661 aatataccaa ctgctgaaaa cagatcggga atgaactact caaaatacag taatccaaca
      721 tacgacaaat tgatagagga aggaaataaa gaatcaaata ttgggaagaa attgagtttg
      781 tttaacaagg cagaaataga attactcaaa aaccctccgt tcatgtcgct atggtacaat
      841 ggcgatatag aaataaccta ttcttatgtt cgtaatttac actttaattc tttaagttat
      901 tttaaattta aaaaggtata tttgaagcct tggactgcag aagagtacca aaaagaaatt
      961 acgaataaga aataaagtaa actatgctaa aattatcttc gctgctagta tttttattac
     1021 tggcagttct ttcatttgca caacctgttc aaaatgtgcg tggtagagtg tttgattctg
     1081 aaacttttac ccctgtcgtt gctgcaaaag ttgtgatacc ttcaaaagat actactaaag
     1141 tttatcaagc attaactgac gataaaggtg aatttttaat tagcaatgta gtcgttggga
     1201 aatataccct acacgtcaac ttttttggtt acgatgaaaa aacggtaaac ctagaagtta
     1261 tttcaggtaa agaatcaatt attgacgttc caatgactga agcttatgtg aaaaataccg
     1321 acgaagtagt ggttactgct cgaaaatcag gtgaagttat caatgaattg gcgttgattt
     1381 ctgctcgtca gtttagtgta gaagaaacca atcgttatgc aggttcacgc tccgatcctg
     1441 ctcgtatggc ttccaatttt gcgggcgtgc aaggtgctga cgactcacgt aatgacatta
     1501 ttgttagagg aaactcgcct cttggtgtgg tttggaaagt ggaaggaata gacattccca
     1561 acccttcgca ttttgctact tctggtagta cgggcggacc ggtaactatt ttgaacaata
     1621 aaattcttgg aaattcagac ttttttatga gtgcctttcc agctgaatac ggaaatagta
     1681 cagccgccgt gttcgattta aaattaagaa actgtaataa tagtaaccat gaatttacag
     1741 gtcagtttgg gtttttaggt acagaattgt tggccgaagg tcctatgagc aaaactggaa
     1801 attcgagtta tttgataatg ggaaggtatt ctaccttgag tcttttcaaa atgatgggaa
     1861 tcaaaattgg aacggatgca gtgccagaat acggcgatgg tgcttttaaa tttaattggc
     1921 gttttaaaaa tggaggtaac ctctcgtttt ttggaattgg aggaaaaagc aacatccaaa
     1981 tcatgatttc tgaacaaaaa gagttctctt cagaaatgta tggtgaaggt gatcgcgatc
     2041 aatattttgg aacgtcgatg gtggtttctg gtttcaacta caaacaatca ctttctgaaa
     2101 aaacattttt aaccactacc cttgcctaca gttacgatga acaacattcg catcacaatt
     2161 atttattacg tcatttggat accttgaaca aaggtactgc tgacgaagct tatcaaattg
     2221 ccattgattc catttatcca atgatgggct atcaatttca atttcacaaa gttgcatacc
     2281 atttagcagt aacacataaa ttcaatacac gccacttaat aaaaacaggg attaacaccg
     2341 atttgtatta tgtagagcaa aaagattctg ctttgcgctt ggtaacggac aatcaatttc
     2401 aaatgcgttg ggactacaaa ggagcataca ctttgattca accatttatt cagtggaaat
     2461 ggcgtgcaac cgaaaacatg gacgtaactg ctggtttgca tgcgcagtat ttttctttga
     2521 gcaatcgttt tagtggtgta gaaccacgtt tgggttggcg ttggaaaatt aaaaacaacc
     2581 acagtgtatt tgcaggagcg ggtatgcaca gtcaaactca acctttatat acatacactt
     2641 ataacaaatt tgatgctgcg ggcaataaaa tctaccacaa caaaaacatg gattttagta
     2701 aaagtatcca cacaggaatt ggttacgaag cattcttgcc aaaaagctgg aacattaagt
     2761 ttgaagcata ttaccaatac ttgtacaacg ttcctgtaac ggttactcct tctgcattct
     2821 ctctaatcaa catgggggca ggatttcaac gctttttccc agatacactt aaaaactctg
     2881 ggacaggcag aaattatggt tttgagttaa ccgttcagaa atttttcgat aaatcattct
     2941 ttgtattggt tacagcatct gtatatgact cacaatacaa aggcagcgat ggtgtaatga
     3001 gaaatacgtc ctacaacggc aactatgtat ttaatgtttt gggaggtaaa gaatttaaaa
     3061 tcggtaaaaa acaatctatt tcaaccggaa ttaaagcaac ttatgcaggc ggacaacgct
     3121 atggatatgt taacattcct gaatcagaaa aataccatga attaatatat gcaagcgatg
     3181 gctacaattc tagacgattt aaagattatt tccgtttgga tttgaaatta agctggaaat
     3241 acaatgctcg agtattgact cacgaaattg gtttagattt ggtaaacatc ttaaatacca
     3301 aaaacgtgtt gagtttaact tacgcgccta atttagcaga tccaactgca gaacctttgg
     3361 caacaaaaaa ccaattggga ttcttgccgt tgttttacta taaaatagat ttcagattta
     3421 ctaaaaagaa gaattaatca gcattttttg aaaggaggtt gcgcaaaaat tgaacgttta
     3481 ccactttaaa aagaagcaaa caaataccaa tgaatattcc ttgtaacgct atgttcaagg
     3541 aatatttttt ttgtagccaa agtccgccaa aaacaacaat gcaatacaat ggaaacacca
     3601 aaatactttt caatgaaaaa ctaaggttga gtaattttac aactacaaca aactctgcaa
     3661 aagccacaaa cgattgagta atcaacgttg cataggccgc tccaactact ccataagatg
     3721 gaatgagcac taagttgata gcaatgttta atacaagtcc aattcctgca ataatattaa
     3781 gaatacgcat gtttcctagt gctgtaagat aagtcccaaa aatcagtgtg atgcacatgg
     3841 agataaacgt aaacattagc aattgaaacg gcaaaactgt tgatgaaaca tcgttgttgt
     3901 agattaagtc aagcaagtca aacgcattcc aaaaacacaa tgccgttaat gcaatagagc
     3961 cccaaatcaa taatttggaa gaagacaaca acaatgtttg tacgttttct tttgttgcaa
     4021 tcatttttga gaatagcggc aacaacaagt tagaaaacaa catagcaaac ataaaaaagg
     4081 catccagcaa gcgaaaacct tgtgcataaa taccggcttg tttagcgcca tcgggcaaca
     4141 tgcgttctat catcaccgaa tcaattcggg taaaaagcat catcaaaata atcagcaagg
     4201 cataaggcaa actttggcgt gtaattaaat acgaaatgtg tttattgatg gaaaccttga
     4261 tttttcctgc atttttcact accaaaacca agcccaccaa aaaggaaata gcataactgg
     4321 caaattgcaa ccaaataaat gtggtaattg taaaattgga aaccgtatgc aaatacaaag
     4381 cacttccaca cagcacaatc aataaaaaac gatctaaaac agacagcaaa gcatccaatt
     4441 taaacagcaa taatcccgat aaataactac gaaaataagc aatcattccg atgatgaatt
     4501 gctgcacaat caacaccgtc aaaataggat aaacagaaac atcgtattgt aaaatcagtg
     4561 ctaaaccaag tgtgataaac gcataaacaa taaaaaacac caagcgcaaa cctgcaattt
     4621 tacctatgta ttttttcatc agcgctggat actgtgcaac atttttagtg gtgtaattgt
     4681 tgatgccaaa atccaataaa atattgaata agtaggtgaa attcaacaaa ctaaaataca
     4741 aaccataatc agcagaacct actctatttt gcacagcggc atcaattcca aaaattgaaa
     4801 ttggttttac aatcaaattg agtaaaacaa gtagtacaag attggatata aatttctttt
     4861 gcaaatgaac ggttatttaa aaatgaaatt ttgagttatg tgtttctttt catcaaacaa
     4921 aactaattta tcattttcaa taacgtaaac cgaataacca atatttttta acaaatcaaa
     4981 actatttttt cgattgtcgt tatcccacaa ttcggcataa ataattggtt ttttatctct
     5041 taaaagttgc tcaccaccta aaaaagcgaa atattcaaaa ttttcaatgt caatttttat
     5101 agcctggatg ttatcgccca aaaacaattc atccaaggtt tttacgggca cttcaaactc
     5161 ttcacctttg ttaaaagaag gaatagattc gtgcaaaacg tgggcaagtc cttgcatttt
     5221 ggttttccca ttgtagggca ttaccatttt taattttcct ttagattgac cgagtgcaat
     5281 aggatacact tccacatttt tcaattgata gcgtgcaatg atgcgtttta acaccgaaaa
     5341 attcaccgga attggctcaa tggaatgtac ttttaaattc ggaaatttag aagccaaata
     5401 ataggtcatt acaccaatgt tggctccaat atcaattaaa tcgccttttt tatcttggat
     5461 taaatttaaa aaatgaaaaa aatctttttc gttagcatca tttttcagcg tattgatttt
     5521 aaattttgaa aaaatataca aatat
//
LOCUS       DAOQVQ010000116         5513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16262_length_5513_cov_3.228289, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000116 DAOQVQ010000000
VERSION     DAOQVQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16262_length_5513_cov_3.228289"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..581
                     /locus_tag="PKN22_04895"
     CDS             48..581
                     /locus_tag="PKN22_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNS42075.1"
                     /translation="MRVERKIVHKERPVLSEEAKAEIQKVISYYPEGKKKSAILRTLF
                     IVQREAGGWLSADTMDMVAEILEITPIEVYEVATFYTMFKLEPVGQYVLEFCHTGPCM
                     LVGSDQLIKYTEEKLGIKVGETTPDGMFTIKVAECLGACGYGPMLQCGKHYHEHLTPE
                     KMDEFLEACRRGEVKTN"
     gene            588..1928
                     /gene="nuoF"
                     /locus_tag="PKN22_04900"
     CDS             588..1928
                     /gene="nuoF"
                     /locus_tag="PKN22_04900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNS42076.1"
                     /translation="MGRKLLLEHINVPGIETFDVYRKHGGYASVEKALKTMAPEEVVE
                     EVKKSGLRGRGGAGFPTGLKWSFIAKPEGVERHLLCNADESEPGTFKDRYLMQYIPHL
                     LIEGMIVSSYALGANLSYIYIRGEYMFVLDILEKAIAEAYEKGFLGKNILGTGYDLDL
                     RVSPGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGLWGRPTVVNNVESIATVVP
                     IINMGADEYAKIGTEQSHGTKLISACGNVERPGVYEIELGMSVEEFIYGDDWCRGIKN
                     GKKLKALIPGGSSVPILPHYLITKTAKGETRYMSYESLSDGGFATGSMLGSGGFIVFD
                     EDQCIVRNTWNFARFYAHESCGQCSPCREGTGWMDKVLHRLEHGKGQMRDIDLLEKVN
                     KRIEGNTICPLGDAAAWPVAAAIRHFREEFEWHVTNPEEAQKHNFGLAKEVTFS"
     gene            1973..2947
                     /locus_tag="PKN22_04905"
     CDS             1973..2947
                     /locus_tag="PKN22_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNS42077.1"
                     /translation="MVKVTIDGVEIEVEEGTTILNAARQAGGDIVPPAMCYYTGLEGS
                     GGKCRSCLVEVAAGSERDPRPMPKLVASCRTEVQHGMVVKNKSSEKVIDARQGVTEFL
                     LINHPLDCPICDQAGECDLQDLSFKHGVEWSRFEEEKRTFAPRDIGDKIKLHMNRCIM
                     CYRCVFTAQQLTNKRVHGVLNRGEHSEISTYIENALDSEFIGNVIDVCPVGALTDKTF
                     RFKSRVWFLKPMNAHRDCPTCQGIATVWMFGNEIYRVTGRKDKYSEIERHNGKTAWIC
                     NTCRFETKDVNQWVIEGPRVINRYSVISTNHYVYNNPLDSKKDTKKIQ"
     gene            2947..4056
                     /gene="nuoH"
                     /locus_tag="PKN22_04910"
     CDS             2947..4056
                     /gene="nuoH"
                     /locus_tag="PKN22_04910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNS42078.1"
                     /translation="MGAEVLIEKGVLVVVLFAITLGVAAYLTLMERKVAAWMQNRIGP
                     DRAGKWGLLQPLADGGKLFFKEDFIPTNADRVLFIIGPGLSMGFALMTSAVIPWGPNI
                     VMFGKEIALQVTDINVGMLFVMALSSVAVYGIMIGGWASNNKYSLFSAIRASSQMISY
                     ELALGISIITVVMLSGSLSLRDIVESQHGMNWNIWYQPLGFIIYFVCLLAELNRAPFD
                     MAECESELITGHHTEYGAMKMGFYLFAEYTHMFIGAAIISLLFFGGYNFPGMDHFSGN
                     TLAILGTLVFFAKTAFFIFLIIWVRWTLPRFRYDQLMHLGWKKLIPLSIANLMITAIV
                     VAATSGWFSDDAANEKTTAKVENVVASAPANKMNK"
     gene            4125..>4571
                     /locus_tag="PKN22_04915"
     CDS             4125..>4571
                     /locus_tag="PKN22_04915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNS42079.1"
                     /translation="MTFMESLYLPVIAAGMASTFKHMFQKPVTYRTPEQKREFSPYYR
                     GWHVLKRDEEGRENCTACGLCAVACPAEAITMVAAERKPGEEHLYREEKYAETYEINM
                     LRCIFCGLCEEACPKDAIYLTISKELVPANYDREDFIFGKDKLVMPL"
     assembly_gap    4572..4581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4582..4731
                     /locus_tag="PKN22_04920"
     CDS             <4582..4731
                     /locus_tag="PKN22_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNS42080.1"
                     /translation="KEAIFLTDRLVPTYFERQSFVYGKDKLVEDMNNRIDITKRQHTG
                     KRGKR"
     gene            4728..5243
                     /locus_tag="PKN22_04925"
     CDS             4728..5243
                     /locus_tag="PKN22_04925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNS42081.1"
                     /translation="MSLIEILALVLGGLTIASGLMVVLSHHPVRSVLYLILTFFLISA
                     NYILLNAQFLALVNIVVYAGAIMVLFLFVLMLLNLSKDNEPKASSILKLSASIAAGIL
                     VIVLVAALKDTSMITMGAAAPDNGQGLVENLGNLLFTKYVLPFELSSVLFIAAMVGAV
                     LLSKREKQIID"
     gene            5247..>5513
                     /gene="nuoK"
                     /locus_tag="PKN22_04930"
     CDS             5247..>5513
                     /gene="nuoK"
                     /locus_tag="PKN22_04930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNS42082.1"
                     /translation="MILATMLNSGIALEYYMILATILFTIGVLGVLIRKNAIIILMCV
                     ELMLNAVNLLLAAFSTYYGKGDAQVFVFFIMVVAAAEAAVGLSIL"
BASE COUNT         1720 a          804 c         1280 g         1699 t
ORIGIN      
        1 cgttgagtag tttaaatgtt attgctggag aattggatgc ttaagttatg agagtagaaa
       61 gaaaaattgt acataaagaa cgacctgttc ttagtgaaga agcaaaagca gaaatccaaa
      121 aggtaattag ttattaccct gaaggaaaga aaaaaagtgc gattcttcgt accttgttta
      181 ttgttcaacg tgaagccgga ggttggttga gtgctgacac aatggacatg gtggctgaaa
      241 ttttggaaat tacaccaatt gaagtgtatg aagttgctac tttttatacc atgtttaaat
      301 tggaaccagt tggacaatat gtacttgagt tttgtcatac aggaccgtgc atgttggttg
      361 gaagtgacca attgattaaa tataccgaag aaaaattggg aattaaagta ggtgaaacta
      421 ctcctgacgg gatgtttact atcaaagttg cagaatgttt gggtgcatgt ggttacggac
      481 caatgttgca atgtggaaaa cattatcatg aacatttaac tccagaaaaa atggacgagt
      541 tcttagaagc ttgccgtaga ggagaagtta aaactaatta atctgtcatg ggaagaaaat
      601 tattattaga acatattaac gttcctggaa ttgaaacttt cgatgtttac cgtaaacacg
      661 gaggatatgc ttctgttgaa aaagcattga aaacaatggc accagaagaa gtggtagagg
      721 aagttaaaaa atcaggattg agaggtcgtg gtggagctgg tttccctaca ggtttaaaat
      781 ggtcattcat tgcaaaacca gaaggtgttg aaagacactt gttgtgtaat gccgatgaat
      841 cggagccagg aacatttaaa gatagatatt taatgcaata cattccgcac ttgttgattg
      901 aaggaatgat tgtttctagt tatgcattgg gtgccaattt atcttacatc tatattcgtg
      961 gagaatatat gtttgtgttg gatatattgg aaaaagccat tgcagaagcg tatgaaaaag
     1021 gatttttggg taaaaacatt cttggaacag gatacgattt ggatttgaga gtttctccag
     1081 gagcaggtgc ctacatctgt ggagaagaaa ctgccttgat tgaatcgttg gaaggaaaaa
     1141 gaggaaaccc gcgtatcaaa ccaccatttc cagctgtaaa aggattatgg ggaaggccaa
     1201 cggtggtcaa caacgttgaa tctattgcaa ctgttgttcc tattatcaac atgggagcgg
     1261 atgagtatgc gaaaattggt acagaacaaa gtcatggtac aaaattgatt tcagcatgtg
     1321 gaaacgttga acgcccaggt gtgtatgaaa ttgaattagg tatgtcggta gaagaattta
     1381 tctacggtga tgactggtgt agaggaatta aaaatggtaa aaaattaaaa gcgttgattc
     1441 ctggaggttc ttcggttcca attttaccac attatttgat tactaaaacc gcaaaaggtg
     1501 agacacgtta tatgagctat gaatcacttt ctgatggtgg ttttgcaacg ggttcaatgt
     1561 tgggttctgg aggtttcatt gtgtttgatg aagaccaatg tattgtaaga aatacgtgga
     1621 attttgctcg tttttatgca catgaatcgt gtggtcaatg ttcaccatgc cgtgaaggta
     1681 caggatggat ggacaaagtg ttgcaccgtt tagagcatgg taaaggacaa atgcgcgaca
     1741 ttgatttgtt ggaaaaagta aacaaacgca ttgaaggaaa caccatttgt ccgcttggag
     1801 atgctgctgc ttggcctgtt gctgctgcaa tacgtcattt ccgcgaagaa tttgaatggc
     1861 acgtaaccaa cccagaagaa gcacaaaagc acaattttgg tttggcaaaa gaagtaactt
     1921 ttagttaagt tgctgtaaat tagtgatttt tagacaataa aaaacagaag atatggtcaa
     1981 agtaaccatt gatggagtag aaatagaagt agaagaggga acaacgattc tcaacgctgc
     2041 tcgtcaagct ggaggtgaca tcgttccacc agcaatgtgc tactataccg gtttggaagg
     2101 tagtggtggt aaatgtcgtt cttgtttggt tgaagttgcc gcaggatcgg agagagaccc
     2161 gcgtcctatg cctaagttag tagcttcttg ccgcaccgaa gtgcaacacg gaatggtggt
     2221 taaaaacaaa tcaagcgaaa aagttattga tgcccgccaa ggtgtaacag aatttttgtt
     2281 aatcaaccac ccgttggatt gcccaatttg tgaccaagca ggtgagtgtg atttgcaaga
     2341 tttatcgttc aaacacgggg tagaatggtc acgttttgaa gaagaaaaac gtacgtttgc
     2401 tccacgtgat attggtgata aaattaagtt gcacatgaac cgttgcatta tgtgttaccg
     2461 ttgtgttttt acagcgcaac agttaactaa taaacgtgta catggtgtgt taaacagagg
     2521 tgaacactct gaaatcagta cgtatattga aaatgctttg gacagtgaat ttattggaaa
     2581 cgtgattgac gtttgtccag ttggagcatt gaccgataaa acattccgtt tcaaaagccg
     2641 tgtttggttc ttaaaaccga tgaatgcaca cagagattgc cctacttgtc aaggtattgc
     2701 aacggtgtgg atgtttggta atgaaattta tcgcgtaaca ggtagaaaag acaaatacag
     2761 cgaaattgaa cgtcacaacg gaaaaacagc ttggatttgt aacacttgtc gttttgaaac
     2821 aaaagacgtt aatcaatggg tgattgaagg accacgtgtt attaatcgtt actcggtgat
     2881 ttcaacaaat cattatgtgt acaacaatcc attggattct aaaaaagata ctaaaaaaat
     2941 acaataatgg gtgcagaagt attgattgaa aagggtgttt tagttgtcgt tttatttgca
     3001 ataacactag gtgttgctgc atatttgaca ctaatggaac gtaaagttgc agcttggatg
     3061 caaaaccgta ttggtccaga tagagctgga aaatggggtt tgttacaacc attggcggat
     3121 ggtggaaaat tgttctttaa agaagacttt attcctacca atgcggatag agttttgttt
     3181 atcatcgggc caggattgtc aatgggattt gccttgatga catctgctgt tattccttgg
     3241 ggaccaaata ttgtgatgtt tgggaaagaa atagcgttgc aagttactga tatcaacgtg
     3301 ggaatgttgt ttgtaatggc actttcttcg gttgcagtgt atggaattat gattggtgga
     3361 tgggcatcga ataacaaata ttcattgttt tctgcaatcc gcgcatcttc gcaaatgatt
     3421 tcttatgaat tggcattggg catttcaatt attacggttg tgatgctttc tggaagtttg
     3481 agcttgcgcg atatcgttga atctcaacac ggaatgaact ggaatatttg gtatcaacca
     3541 cttggattta tcatttattt cgtgtgtttg ttagcagaat tgaaccgtgc accgtttgac
     3601 atggcagaat gtgagtcgga gttgattact ggtcaccata cagaatatgg agcaatgaaa
     3661 atgggattct atttatttgc tgaatatacc cacatgttta ttggagcggc aattatttct
     3721 cttcttttct ttggtggtta caactttcca ggaatggatc atttctcagg aaatactttg
     3781 gcaatattag gaacattggt gttctttgct aaaacagcat ttttcatttt cttgattata
     3841 tgggtgcgtt ggacattacc acgttttcgt tacgaccaat tgatgcattt gggttggaaa
     3901 aaattgattc cactttcaat tgctaactta atgattactg caattgttgt ggcagcaact
     3961 tctggttggt ttagtgatga cgcagcaaat gagaaaacta cggccaaagt agaaaatgtg
     4021 gtggcaagtg caccagccaa taaaatgaat aaataaaaac gataattact agaaatagca
     4081 tggaaagttt atcgaataga aaaaaagtag tatccaacaa aaaaatgact tttatggaaa
     4141 gtctttacct acctgtaatt gctgcaggta tggcatctac ttttaaacac atgtttcaaa
     4201 aacctgtaac ctatagaact ccagagcaaa aaagagaatt ttctccttac tatagaggtt
     4261 ggcatgtatt gaaacgtgat gaagaaggac gtgaaaactg tacagcctgc ggattgtgcg
     4321 ctgttgcttg tcctgccgaa gcaatcacaa tggtggctgc agaaagaaaa ccaggagaag
     4381 agcatttgta cagagaagaa aaatatgcag aaacatacga aatcaatatg ttgagatgta
     4441 ttttctgtgg tttgtgcgaa gaggcttgtc ctaaagatgc tatttattta actatttcaa
     4501 aagaattagt tcctgctaat tacgatagag aagactttat ttttggaaaa gataaattgg
     4561 taatgccttt annnnnnnnn naaagaagca attttcttaa ctgatagatt ggtgcctact
     4621 tattttgagc gtcaatcatt tgtttatggc aaagataaat tggtggaaga tatgaacaat
     4681 cgtatcgata ttacaaaaag acaacacaca ggtaaaagag gtaaaagatg agtttaattg
     4741 aaattttagc attggtattg ggaggcttaa caattgcatc aggattgatg gttgttttga
     4801 gtcatcatcc cgttagaagt gtgttgtatt tgattttaac attcttcttg atttcggcaa
     4861 attacatttt gttgaatgcg caatttttag cattggtaaa cattgtggtg tatgcaggag
     4921 caattatggt attgttctta tttgtattga tgttgttgaa tttaagcaaa gacaacgaac
     4981 caaaagcatc tagcattttg aagctttcag catcgattgc agcgggtatt ttggtaattg
     5041 tgttggtggc tgctttaaaa gatacttcca tgattacaat gggtgcagca gcaccagata
     5101 acgggcaagg attggtagaa aacttgggta acttgttgtt tactaaatac gtgttgccat
     5161 ttgagttgtc ttctgtattg ttcattgcag caatggtagg tgccgtttta ttgtcgaaaa
     5221 gagaaaaaca aataattgat taagaaatga tattagcaac gatgttaaac tcgggaatag
     5281 cgttggagta ttacatgata cttgcaacta ttttattcac gattggagtt ttaggcgtat
     5341 tgattcgcaa aaatgcaatc attattctta tgtgtgttga gttgatgtta aatgctgtta
     5401 acttattgtt ggcggccttc tcaacttact atggtaaagg cgatgctcaa gtattcgtat
     5461 tctttattat ggtagtagca gccgctgaag ctgcagttgg tctttctatt ttg
//
LOCUS       DAOQVQ010000117         5506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16280_length_5506_cov_3.469272, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000117 DAOQVQ010000000
VERSION     DAOQVQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16280_length_5506_cov_3.469272"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKN22_04935"
     CDS             complement(<1..329)
                     /locus_tag="PKN22_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNS42083.1"
                     /translation="MSLSEKYNLEILHEDNHTIVVNKKASDIVQGDKTGDEPLPERIK
                     KYLKEKYQKPGNVFCGVVHRLDRPTSGALVFARTSKALERLNEQFREKETQKTYWREM
                     LRDMIEHR"
     gene            complement(329..1144)
                     /gene="panB"
                     /locus_tag="PKN22_04940"
     CDS             complement(329..1144)
                     /gene="panB"
                     /locus_tag="PKN22_04940"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNS42084.1"
                     /translation="MSVHSNIKRITTKTLSEMKQRGEKITMLTGYDFSMAKIIDAAGI
                     EVILVGDSASNVMAGHETTLPITLDQMIYHASSVVRACQRALVVVDMPFGSYQGNSKE
                     ALSSAIRIMKESGGHAVKMEGGAEIIESISRILTAGIPVMGHLGLTPQSIYKFGSYVV
                     RAKEEEEAKRLIEDAKLLEEAGCFAIVLEKIPAHLAKKVAESVSVPIIGIGAGNGVDG
                     QVLVVHDMLGINNEFSPRFLRKYNNLYDQMMTSFSRYIEDVKSGDFPNADEQY"
     gene            1273..2055
                     /locus_tag="PKN22_04945"
     CDS             1273..2055
                     /locus_tag="PKN22_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS42085.1"
                     /translation="MQENSFKVTVLGSGTSQGVPVIACDCEVCRSTNAKDKRLRSSIL
                     LTIDGLNYVIDTGPDFRQQMLRANVQELEAIFFTHQHKDHIAGLDDVRAFNFKQQKDM
                     KIYCDVRVEEALHREYEYVFSSFKYPGIPLLDLEIIDKNALIKLPNGNEIRAIEAIHY
                     KLPVLGFRYQNFTYLTDVKTIEPEEIEKIKGSEVLIVNALRKEEHISHFNLQEALDFI
                     ALVQPKTAYLTHISHLLGMYDEVEKELPANVHLAYDGLELIF"
     gene            complement(2052..2711)
                     /locus_tag="PKN22_04950"
     CDS             complement(2052..2711)
                     /locus_tag="PKN22_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNS42086.1"
                     /translation="MSYQEIFQSIPSTVTLVAVSKTKPVEAILEIYNQGQRVFGENKV
                     QEMCDKQAVLPQDIQWHLIGHLQTNKVKYIAPFVTMIHAVDSLKLLAEINKQAKKHNR
                     IIDCLLQFHIAQEDTKFGLDTQEAKELLESTEFQAMENIRIRGVMGMATFTEDEAQIH
                     NEFKALATIFNSLKATYFSEQLTFDTRSMGMSGDYQIAIEEGSTMVRIGSLIFGNRNY
                     N"
     gene            complement(2732..3376)
                     /gene="nth"
                     /locus_tag="PKN22_04955"
     CDS             complement(2732..3376)
                     /gene="nth"
                     /locus_tag="PKN22_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNS42087.1"
                     /translation="MTKKEKAQYIIEELERIYPTTPIPLDHKDPYTLLIAVLLSAQCT
                     DVRVNQITPLLFKRADNPFDMIKLTVDEIREIIRPCGLSPRKSQAIFDLSHLLINQHN
                     GEVPQSFEALEKLPGVGHKTASVVMSQAFGVPAFPVDTHIHRLLTRWGITSGKNVEQS
                     EKDAKALFPKHLWNKLHLQIIFYGREYSPARSPKFEKDSITRTIGTKAALAELV"
     gene            3451..3687
                     /locus_tag="PKN22_04960"
     CDS             3451..3687
                     /locus_tag="PKN22_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42088.1"
                     /translation="MDNKVNDFPQFRQMKGRKVYYKILSASEFIELSWIGEQKMVHRV
                     VATQYPEMLRIMDMLQCVEPFDYLENTKQDEFIR"
     gene            3770..4258
                     /locus_tag="PKN22_04965"
     CDS             3770..4258
                     /locus_tag="PKN22_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNS42089.1"
                     /translation="MNKKFVYGLMFGAAFIFASCGGGEKPAEEAPAADATTEEVKEDV
                     SDYDPNRGEGKFDENNLQITGFDAAMAKKGEAAATTKCYSCHKMTDERLVGPGWKDLT
                     KRRTPHWIMNFITNPDAMIDKDPALQEQLELCMVRMPNQNLADQEARDILEYMRQVDG
                     AK"
     gene            4349..4723
                     /locus_tag="PKN22_04970"
     CDS             4349..4723
                     /locus_tag="PKN22_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNS42090.1"
                     /translation="MVKKDLKKRKDIEKVVGQFYKKVNNDEQLSLFFNEVIKINWERH
                     IPHMCDFWENVLFFKGNYEGNPLAVHRDVHNKHKTNDVHFNKWVQLFEQTVDAYFLGE
                     NADKMKAHAQSIAVLMLQNIEK"
     gene            complement(4728..5174)
                     /locus_tag="PKN22_04975"
     CDS             complement(4728..5174)
                     /locus_tag="PKN22_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNS42091.1"
                     /translation="MFSKSTEYALRATIYIALKSSEEKKIGIVAIAKGINAPQSFTAK
                     ILQNLTNKKNGFIASMPGPSGGFYIRNADRKLPIYRVIEAMDETDVIEDCILGFPTCS
                     DETPCSMHKAYKPLKLELLKILKEKTIDDLANGEDSLLRSGFVSFD"
     gene            complement(5193..>5506)
                     /locus_tag="PKN22_04980"
     CDS             complement(5193..>5506)
                     /locus_tag="PKN22_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNS42092.1"
                     /translation="DVNLVLVSIDPEHDTPEVLKKFAETRRMTEPHWVFLRSNEDATQ
                     EFANVLSMKYKKISPVDFSHSNIISIFNPLGELVVQEEGLVINSKKIASKVNEVVSKN
                     K"
BASE COUNT         1744 a          962 c         1015 g         1785 t
ORIGIN      
        1 cggtgctcga tcatgtcgcg gagcatttcg cgccagtatg ttttttgggt ttctttttca
       61 cgaaattgtt cgtttaatcg ctccaatgcc ttacttgttc ttgcaaacac caaagcacca
      121 cttgttggtc ggtctaatcg atgtaccaca ccacaaaata catttcctgg cttttggtat
      181 ttctctttca agtatttctt aatgcgttcg ggcaaaggtt catcacccgt tttatcgcct
      241 tgaacaatat ccgatgcttt tttattgaca acaatggtat ggttgtcttc gtgcagaatt
      301 tctaaattgt atttctctga aagtgacatt aatattgctc gtctgcgtta gggaaatcac
      361 ctgattttac gtcttcaatg tatcgagaga atgaagtcat catttgatca tacaagttgt
      421 tgtattttct caagaaacgt ggactaaatt cgttgttaat gcccaacata tcgtgcacca
      481 ccaaaacttg accatccaca ccatttccag caccaatccc aataattgga acggaaacag
      541 attctgctac ttttttagct aaatgagcag ggattttctc taaaacaatt gcaaaacaac
      601 ctgcttcttc caataattta gcatcttcaa tcaatcgttt tgcttcttct tcttcttttg
      661 cacgcaccac atacgatcca aacttgtaga tggattgagg cgttaacccc aaatgcccca
      721 ttacaggaat gcccgcagta agaatacgag aaatagattc aataatttcg gctccacctt
      781 ccatttttac ggcatgacca cccgattctt tcataatgcg aattgcactc gataaggctt
      841 cttttgaatt tccttgatac gaaccaaatg gcatatctac aactaccaac gcacgttggc
      901 aagctctaac aaccgaagag gcatgataaa tcatttgatc caaggtaatt ggaagtgttg
      961 tttcatgtcc agccatcaca ttcgaagctg aatcgcctac taaaattact tctataccag
     1021 ctgcatcaat gatttttgcc attgagaaat cgtagccggt caacatggtg attttttcac
     1081 cgcgttgttt catttcgctt aaagttttag tagtaattcg cttgatgttt gaatgaactg
     1141 acataccttt tgttttaaaa ttattttgca aaggtataaa aactattttg gaagataaga
     1201 aataaagtta tttcaaaaga actattgctt tccattcaga aaacattaat tttagccttg
     1261 aattgaagta ggatgcaaga aaattcattt aaagttacgg ttttgggttc tggtacatcg
     1321 caaggagtgc cggtaattgc ttgtgattgt gaagtgtgca ggtcaaccaa tgcaaaagat
     1381 aaacgattgc gttcttctat tctgcttacc attgatggat tgaattatgt gattgacacc
     1441 ggtccggatt ttcgtcaaca aatgcttcgt gcaaatgttc aagaacttga agccatattt
     1501 tttacccacc aacacaagga tcatattgca ggattagacg atgtgcgtgc ttttaacttc
     1561 aaacagcaaa aagacatgaa aatctattgc gatgttcggg tagaagaagc cttgcaccgc
     1621 gaatacgaat atgttttttc atcgtttaaa taccctggaa ttccattgct cgatttggaa
     1681 ataattgata aaaatgccct tatcaagctg cctaatggaa acgaaatacg cgccatagaa
     1741 gccattcact acaaattacc tgtgttggga tttcgttacc aaaactttac atatttaaca
     1801 gacgtaaaaa caatagaacc cgaggaaatt gaaaaaatta aaggctcaga agtgttgatt
     1861 gttaatgctt tgcgaaaaga agaacacata tcacacttca atttgcaaga agccttggat
     1921 tttattgcgc tcgttcaacc aaaaactgct tatctaacgc atatttcaca ccttttggga
     1981 atgtacgatg aggtagaaaa agaattgcct gccaacgttc acttagccta tgacgggttg
     2041 gaacttatat tttaattgta atttctgttt ccaaaaatta aactaccaat tcttaccatg
     2101 gtgctgcctt cttcaattgc aatttggtaa tcaccactca tccccattga acgggtgtca
     2161 aaggttaatt gctcactaaa ataagttgct tttagagaat tgaaaattgt tgctaatgct
     2221 ttaaattcat tgtgaatttg ggcttcatct tctgtaaatg ttgccattcc cattacacca
     2281 cggattcgga tgttttccat agcttggaat tctgtagatt ccaaaagttc tttagcttct
     2341 tgtgtatcca atccaaattt ggtgtcttct tgtgcaatgt gaaactggag caaacaatca
     2401 atgatgcggt tgtgtttttt tgcttgtttg ttgatttctg ccaataattt tagactatcc
     2461 accgcgtgaa tcatcgtaac aaaaggggcg atgtatttaa ctttattggt ttgcaagtgc
     2521 ccaatcaagt gccattgaat atcttggggc aaaaccgctt gtttatcgca catttcttgc
     2581 actttatttt caccaaaaac acgctgacct tggttgtaaa tctccaaaat agcctcaaca
     2641 ggttttgttt ttgataccgc aacaagtgtt acagttgacg ggatggattg aaaaatttct
     2701 tgataactca tagcaattca aaggtaatga tttaaactaa ttctgcaagg gctgcttttg
     2761 tgcctatggt gcgtgtgatg ctgtctttct cgaattttgg acttcgagca ggcgagtatt
     2821 cgcgaccata aaaaatgatt tgcaagtgta atttattcca caaatgttta ggaaataaag
     2881 ctttggcgtc tttttcagat tgttccacat tttttccaga ggtaattccc cagcgagtaa
     2941 gcaaacggtg aatgtgagtg tcaacaggaa aagcgggcac accaaatgct tgtgacatta
     3001 caacagatgc tgttttatgt cctacaccag gcaatttttc aagtgcttca aatgattgag
     3061 gcacctcacc attgtgttga ttaatgagta aatgcgaaag atcaaatatt gcttgtgatt
     3121 tacgaggtga aagtccgcat gggcgaatga tttcacgaat ttcgtcaaca gtaagtttaa
     3181 tcatatcaaa cggattatca gctcgtttaa agagcaatgg agtaatttga ttcacgcgaa
     3241 catccgtgca ttgtgcagaa agcaaaacag caattaataa agtgtaggga tctttatgat
     3301 ccaagggaat tggagtagtg ggatagatgc gttccaattc ctcaataata tattgtgctt
     3361 tttctttttt tgtcatttta tagagctgtt aaacagtgtt tcattagtta aaatggcaat
     3421 tcattcttaa ttttgtaaat aaaattacct atggataaca aagttaatga ctttccgcag
     3481 tttagacaga tgaaagggcg aaaagtatat tataaaattc tttcagcttc ggagttcatt
     3541 gaattgtcat ggataggaga gcaaaagatg gttcatcgtg ttgttgctac tcaatatcca
     3601 gaaatgttgc gaataatgga tatgctgcaa tgcgtggaac cttttgatta tctggaaaat
     3661 acaaaacagg acgaatttat ccgataaaaa aagacatttt tatgacattt atcatagatt
     3721 ataacattta gtttacttat ctttgttgta agaaattaaa atttagatta tgaataagaa
     3781 atttgtatat ggattaatgt ttggagcagc gtttatattt gcttcatgtg gaggaggtga
     3841 aaaaccagcc gaagaagctc cagcagctga tgcgacaaca gaagaagtaa aagaagatgt
     3901 ttctgattac gatccaaaca gaggggaagg taagtttgat gaaaataacc ttcaaattac
     3961 tggatttgat gcggctatgg ctaaaaaagg tgaagcagca gctacaacaa aatgttattc
     4021 ttgtcataaa atgacagatg aacgtttggt tggtccagga tggaaagatt tgacaaaaag
     4081 aagaactcca cactggatta tgaactttat tacaaatcca gatgcaatga ttgataaaga
     4141 tcctgcattg caagagcaat tggagttgtg tatggttcgt atgccaaacc aaaatttggc
     4201 tgatcaagaa gcaagagata ttcttgaata catgagacaa gttgacggag cgaaataatc
     4261 gtttcaaaaa aaatagaaga aatgagtttg gagcaattca aactcatttc ttttttatat
     4321 acttgtagca aaattatgac tacaaacaat ggttaaaaaa gatttaaaaa agagaaagga
     4381 tattgaaaaa gttgttggtc aattttataa aaaagtaaat aatgacgaac aattgtcact
     4441 cttttttaat gaagtcataa aaatcaattg ggagcgccat atcccgcaca tgtgtgattt
     4501 ttgggaaaat gtgttgtttt tcaaaggaaa ttatgaaggt aatccattag ccgttcatcg
     4561 tgatgtgcac aacaaacaca aaacaaacga tgtgcatttt aataagtggg ttcaactatt
     4621 tgaacaaacg gtagatgcat attttttagg agagaatgct gataaaatga aagcacatgc
     4681 tcaatcaatc gctgttttaa tgttgcaaaa tatagaaaag taaattacta atcaaaagaa
     4741 acaaaacccg aacgtagtaa agaatcttct ccgttggcta aatcatcaat agttttttct
     4801 ttcaatatct ttaacaactc gagttttaga ggtttgtatg ccttgtgcat agagcaagga
     4861 gtttcatcag aacaagttgg gaaacctaaa atacaatctt caataacatc tgtttcatcc
     4921 attgcttcaa ttacacgata gatgggtaat tttctatctg catttctgat gtaaaaccca
     4981 ccagaaggac caggcataga tgcaatgaat ccattttttt tattcgttaa attttgcaat
     5041 atttttgcag taaacgattg tggcgcatta ataccttttg caattgctac tattccaatc
     5101 ttcttttctt ccgaagattt tagagcaata taaatggtag ctctaagtgc gtattcggtt
     5161 gattttgaaa acatgatatc gaataatagt tattatttat tcttgctaac aacttcgttc
     5221 acttttgaag ctattttttt agaattgatt accaatcctt cttcttgaac cactaattca
     5281 cccaatggat tgaaaatgct aatgatgttt gaatgcgaga aatctacagg tgaaatcttt
     5341 ttgtatttca tagacaacac gttggcaaat tcttgtgtag catcttcgtt acttcttaaa
     5401 aatacccaat gaggctctgt cattctgcgt gtttcagcaa atttctttaa tacttcagga
     5461 gtgtcatgtt caggatctat tgatactaaa accaaattaa catctt
//
LOCUS       DAOQVQ010000118         5425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16596_length_5425_cov_3.333520, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000118 DAOQVQ010000000
VERSION     DAOQVQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16596_length_5425_cov_3.333520"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKN22_04985"
     CDS             complement(<1..416)
                     /locus_tag="PKN22_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42093.1"
                     /translation="MLRARAFSVHQNQIQLYTTQFEEDKAIIPQGFSVLSNLERSVLD
                     VNPSLKGKKLPLIADILEKRKEIQTFDYLVFTNMDIAVQPYFYDTIFKYIEQGHDAIV
                     INRRRISDKRRTLQDLPLMYADMGKSHPGFDCFVIK"
     gene            506..1630
                     /gene="rffA"
                     /locus_tag="PKN22_04990"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             506..1630
                     /gene="rffA"
                     /locus_tag="PKN22_04990"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNS42094.1"
                     /translation="MKILFNNIGKSTNYLKNIELLYSNYTNFRKKHFSNLCLEHLQKM
                     YPASKLLLTHSATGGLEIIAELLHIQKGDEVIMPSFTFVSTANAFVSKGATPVFVDID
                     KNTLNIDIQQIEEHISHKTKAIVAMHYGGHPCDLKQLRALCDKHHLFLIEDAAMGFGN
                     HYNNQPLGSIGDFGVISFDITKQISAVQGGLLLINNADYVKRAGHIYHIGTNREDFMD
                     GNVPYYEWVDVGSKYQLNELNAAVLLDQLERYPEIIAHRNIVSKLYHYYLKPLELQGK
                     FRLMSDTHLENNCHEYYLILNDQFERDNLSKYLYNYGIETMFHYIPLHNSTKGRSFGH
                     RELPNTIEISESLLRLPMHTELGETHIQYIVNKIKSYWEE"
     gene            1630..2451
                     /locus_tag="PKN22_04995"
     CDS             1630..2451
                     /locus_tag="PKN22_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42095.1"
                     /translation="MSDELHIISSKQDTFREFFIKIWNYRALIWVFAKRDLKVKYSQT
                     MIGLGWTVLQPLTALFIFTFFFGFLLKWETAGIPYPIYVLSGLLGWNFFSYIVSAGTS
                     SVFESAHLIKKIYFPKSIIPFSKVIIAAIELGISIILLVLLMIYYQQTLSWNIVFMPL
                     VLVYNIICALCLVYWISIFTIKKRDLLHLVPFILYFGIWLSPVFFSNDILPVEYRFLL
                     DFNPIANVIEAWRWSLFGFGEFKIIWIFNFFIVILLFLGGLYIYNRKENSFVDNV"
     gene            2617..4206
                     /locus_tag="PKN22_05000"
     CDS             2617..4206
                     /locus_tag="PKN22_05000"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNS42096.1"
                     /translation="MRQKFVIVSSLALAITLALTIYLNKNWGIFFIIVLILTIMGYQD
                     MYQKKHAIRRIFPLFGRLRYVLEELRPKMYQYFIESDTDGKPISRIERSTIYQRAKKE
                     LETVPFGTQLDVYAEGYEWMCHSIAPKHFEETDPRVLVGNKDCKQPYSASILNISAMS
                     FGALSAQAVEAFNGGAKIGGFAHNTGEGGISPYHLKQGGDLIWQIGTGYFGCRDKDGN
                     FNPELFAEKAKFPEVKMIELKISQGAKPGHGGILPAVKNTVEISKIRHVEPHTTVASP
                     PHHTAFDNPIDMMKFIQQLRELSVGKPVGFKLCIGHKAEFISICKAMIELDIYPDYIA
                     VDGGEGGTGAAPQEFTNYLGAPMIDGVAFVHNILNGLDIRKHIKIVASGKISSAFHIA
                     RVMALGADMCYSARGMMMSIGCIQALLCNSNTCPTGITTHDPKLTIGLDVTDKKVRCA
                     NYHKGTITNFMELMGACGIDDMKKITRSHIYRRVSLNDMQTYEEIFPSVKVGAFLNGE
                     ISERYKWDFEHSDAKNWNMVI"
     gene            4328..>5425
                     /locus_tag="PKN22_05005"
     CDS             4328..>5425
                     /locus_tag="PKN22_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS42097.1"
                     /translation="MEKKLWNRDFIIACIANFFIASSFYLLMPTIPIYLSEQLHVPHS
                     EIGIVLSSYVIALLLIRPFSGYMVDVYPRKLLFLFGAGMFVLTYVGYYFAASVAFFFI
                     LRFIHGLFWGLTAVSANTVAIDIIPSERRAEGIGYFGVNMNIAMAIAPFLAVKIYDHF
                     DFNVLVSAALFMGTLAVIAIGLIRVPKRPKMEQKPPISLDRFILVKALPIFFNQLFFA
                     FGYGTLVAYAVLYGKNIGINNSGVFFLFIALGIVLTRITSGKLVDRGYLHQVMLFSTS
                     SLVLGFFCFSVFHTIYAFCFAAFILGIGYGTLFPSLQIMYVNMAPASQRGTANSTYLT
                     GFDLGIGIGMLLGAYLEDHFGFENMYLITAGL"
BASE COUNT         1612 a          918 c         1019 g         1876 t
ORIGIN      
        1 ttttttatta caaaacaatc aaatcccggg tgcgatttcc ccatatcggc atacatcaag
       61 ggcaaatctt gaagtgttct tcgtttatcc gaaatacgtc ggcggttgat gacaatggca
      121 tcgtgtcctt gttcaatgta tttaaaaatg gtatcgtaaa aatacggctg aacagcaata
      181 tccatattgg taaacaccaa ataatcaaac gtttgaatct cttttctttt ttccaaaata
      241 tcggcaatga gtggaagttt ttttcctttt aaacttgggt tgacatccaa cacagaacgt
      301 tctaaattcg acaaaaccga aaaaccttgc ggaataattg ctttatcttc ctcaaattgt
      361 gttgtgtaca attgaatctg attttgatgc acagaaaaag cacgtgcccg taacatcgtt
      421 tcaaaagtta tgggttgcgc gtagtgtaaa tccgattttt cgtttacctt tacgggatta
      481 attatatgtg ctacttgaat caaatatgaa aatattattc aacaatatcg gtaaatctac
      541 aaattatctg aaaaatattg agctactata ctcaaattat acgaatttca gaaaaaagca
      601 tttttccaat ttgtgtttgg agcacttgca aaaaatgtat ccagcctcca aattattgct
      661 tacccattct gctactggag ggttggaaat tattgccgag ttgctacaca ttcaaaaagg
      721 cgacgaagtg ataatgcctt catttacctt tgtttccaca gccaatgcat ttgtttcaaa
      781 aggtgcaact ccagtttttg tggatattga taaaaataca ctcaacattg acattcaaca
      841 aattgaagag catatttcac ataaaaccaa agccattgtt gccatgcatt atggcggtca
      901 cccatgtgat ttgaaacaat tgcgagcact ttgtgataaa catcatcttt ttttaattga
      961 agatgcagcc atgggatttg gcaatcacta caacaatcaa ccattgggct ctattggtga
     1021 ttttggtgtt attagttttg acattaccaa gcaaatatcg gcagtgcaag gtggtttatt
     1081 gctcatcaac aatgctgatt atgtcaaaag agcgggacac atttaccaca ttggcaccaa
     1141 ccgtgaagat tttatggatg gtaacgtgcc gtattatgaa tgggtggatg ttggttcaaa
     1201 ataccaatta aacgagttga atgccgctgt tttattggat caattggaac gttatccaga
     1261 aattattgcc caccgcaaca ttgtttcaaa attgtatcat tattacctca aaccgttgga
     1321 attgcaagga aaattcaggt taatgtcgga cacccattta gaaaacaatt gccatgaata
     1381 ttatttaatt ctcaacgacc aatttgagcg tgataatttg tcaaaatact tgtacaatta
     1441 tggaatagaa accatgtttc actacattcc gctgcacaat tcaacaaaag gacgttcttt
     1501 tggacacaga gaattgccca atactattga aatttccgaa tcattgttgc gtttacccat
     1561 gcacacagaa ttgggcgaaa cacacattca atacattgtt aacaaaatta aaagctactg
     1621 ggaagaataa tgagcgacga attgcatatc atttcatcaa aacaagatac gtttcgtgaa
     1681 ttttttatta aaatttggaa ctaccgtgca ctaatttggg tatttgccaa aagagattta
     1741 aaagtaaaat attcgcaaac gatgattggc ttgggttgga cagttttgca accattgaca
     1801 gcacttttca tttttacatt tttctttgga tttttattaa agtgggaaac agcaggcatt
     1861 ccctatccta tttatgtatt gagtggtttg ctggggtgga atttcttttc ctacattgta
     1921 agtgcaggca catccagcgt ttttgaatca gcccatttga taaaaaaaat ctattttccc
     1981 aaaagcatta ttccgttttc aaaagtcatt attgctgcca tagagcttgg tattagcatt
     2041 attttactcg ttttattaat gatttattac caacaaaccc tttcgtggaa cattgtattc
     2101 atgccccttg ttttggttta caacatcatt tgtgctttgt gtttggtgta ttggatttct
     2161 atttttacca ttaaaaaaag agatttactg caccttgttc cgttcattct ctattttgga
     2221 atttggttat cacccgtgtt ttttagcaac gatattttac cagttgaata tcgcttttta
     2281 ctcgatttta atcccattgc caatgtaatt gaagcttggc gttggtcgct ttttggtttt
     2341 ggtgagttta aaattatttg gattttcaac tttttcatcg ttatcttatt gtttttaggt
     2401 ggtttatata tctacaacag aaaagaaaat tcttttgtgg acaacgtata actctttatc
     2461 cttatttaga atcattctaa taaactcgtt ttttttgtag ttaactattt aagggctact
     2521 tttgcatttt cttttataac ggaaacatct tttgatttgg atagttaaat agttattaaa
     2581 agaaaataat agtgttaatt atttaaaatt aaaagtatgc gtcagaaatt tgtaattgtt
     2641 tctagtttag ctttagctat tacgttggcc ctaactattt atttaaacaa aaattgggga
     2701 atctttttta ttattgtctt gattttaacc attatgggtt atcaagacat gtatcaaaag
     2761 aaacatgcaa ttcggagaat tttcccacta tttggacgct tgagatatgt attggaagaa
     2821 ttgcgtccta aaatgtatca atattttatt gaatcggaca cagatggtaa accaattagt
     2881 agaattgagc gttcaactat ttatcaacgt gcaaaaaaag aattagaaac cgttccattt
     2941 ggtacgcagc tagacgtata tgcggaagga tatgagtgga tgtgtcattc catcgctcca
     3001 aagcattttg aagaaacaga tcctcgcgtt ttggtaggaa ataaagattg taagcaacct
     3061 tattctgcca gtattttaaa tatttctgcc atgagttttg gtgctcttag tgctcaagca
     3121 gttgaagcat ttaatggtgg agctaaaatt ggaggttttg cacataacac aggagaagga
     3181 ggtatcagtc cataccactt aaaacaaggt ggagatttaa tttggcaaat tggtacgggt
     3241 tattttggtt gccgtgacaa agacggtaat tttaaccctg aattgtttgc tgagaaggca
     3301 aaatttcctg aggttaaaat gattgagtta aaaatttctc aaggagcaaa gccaggacat
     3361 ggaggaattt taccagcagt taaaaacacg gttgaaattt caaaaattcg ccacgttgaa
     3421 ccccacacta cagtggcttc tccacctcat catactgctt ttgacaatcc aattgatatg
     3481 atgaaattca tccaacaatt acgtgagtta tcagtaggaa aacctgttgg attcaaattg
     3541 tgtattggtc acaaagcaga atttatttct atttgtaagg ctatgattga gttggatatt
     3601 tatccagatt acattgctgt agatggtggt gaaggtggaa caggagctgc tccgcaagaa
     3661 tttaccaact atttgggcgc tccaatgatt gatggtgttg cttttgtgca taatatcttg
     3721 aatggtttgg atattcgcaa acacattaaa attgttgctt ctggaaaaat tagttcggca
     3781 tttcacattg cacgcgtaat ggcgttgggt gcagatatgt gttattctgc acgtggtatg
     3841 atgatgtcga taggttgtat tcaggcgtta ttgtgtaatt ccaacacctg tccaacagga
     3901 atcacaacac acgatcctaa attaaccata ggtttggatg taaccgataa aaaagtgcgc
     3961 tgtgccaatt accacaaagg aaccattacc aattttatgg aattaatggg cgcatgtgga
     4021 attgacgata tgaaaaaaat tacacgttca cacatttacc gtcgtgtttc attaaacgat
     4081 atgcaaacat acgaagagat tttcccttct gttaaagtag gagctttctt aaacggtgaa
     4141 atttctgaac gctataaatg ggatttcgaa cattctgacg ctaaaaactg gaatatggtg
     4201 atttaatcct attcaaacga aaatctggag ggtagttgca agttgtgact accctctttt
     4261 tttaacaaaa cattaatgcc gtttgccaat attatcacaa agcttagccg tatctttgca
     4321 acaaattatg gagaaaaaac tgtggaatag agatttcatc attgcatgca ttgccaattt
     4381 tttcattgca agctcctttt acttgttgat gccaaccatt ccaatttatt tgtcggaaca
     4441 actacacgtg cctcattctg aaattggaat tgtactatcc tcctatgtta ttgctttgtt
     4501 gctcattcgc ccatttagcg gatacatggt agatgtgtat ccaagaaaac tcttgttttt
     4561 attcggtgcc ggcatgtttg tgttaacgta tgttggttat tattttgcag cctccgtagc
     4621 tttctttttt atattacgtt tcattcatgg tttgttttgg ggattaactg cggtttctgc
     4681 caatacagtt gccatcgata taattccgtc tgaaagaaga gccgaaggta ttggctattt
     4741 tggtgtaaat atgaacatcg ccatggccat tgcaccattt ttagccgtaa aaatctacga
     4801 tcatttcgat tttaatgtgt tggtttctgc cgcgttattt atgggaacct tagctgttat
     4861 tgcgattgga ttgatacgtg tgcccaagcg accaaaaatg gaacaaaaac caccaatttc
     4921 cctcgaccga tttattttag taaaagcact tcctattttt ttcaatcaat tgttttttgc
     4981 ctttggatat ggcacgttgg tggcgtatgc agttttgtac ggtaaaaata ttggcatcaa
     5041 caattcagga gtttttttct tgtttattgc attgggaatt gtgctaacac gcattacttc
     5101 tggtaaattg gtagatagag gttatttgca tcaagtaatg ttgttttcaa caagttcgct
     5161 tgtgcttggt ttcttttgtt tttcggtatt tcatacaatt tatgcatttt gttttgctgc
     5221 atttatttta ggaattggtt atggcacgtt gtttccatcg ttgcaaatta tgtatgtcaa
     5281 catggcgcca gcatcacaaa gaggtactgc caattcaacc tatttaacag ggtttgattt
     5341 aggtattggc atcgggatgt tgttgggcgc ctatttggaa gatcatttcg gatttgaaaa
     5401 catgtattta atcacggcag gactt
//
LOCUS       DAOQVQ010000119         5414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16640_length_5414_cov_4.052622, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000119 DAOQVQ010000000
VERSION     DAOQVQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16640_length_5414_cov_4.052622"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1472
                     /locus_tag="PKN22_05010"
     CDS             24..1472
                     /locus_tag="PKN22_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42098.1"
                     /translation="MNKQLDRSIEMLKKMQVDEKIDAIENELIKLAEDQLALKKEIET
                     KNLDKNKAQEEQSKINEQFNQLKEDLKKLEELNKELNRPMELPSLEKEQNSTEQELNN
                     AQQNLSKGKNKSAGENQQNAAEQMQKMADQMDAAQNEANKEQQGEDMEMLRQILKSLV
                     TLSFEQEQLISNFSSIKSQDPNYRKYGRLQRSIIDKTIPVKDSLYELAKRQPMLSDFV
                     DKELKTIQYNHKQALNNIQERNLRQLNANQQFAMTSYNNLALMLNESLQQMQQNMQAI
                     MKGSGSCSNPGGKGKPSSGEGQLGNMKEQLKKQLEQMKNGSNPGGSEGGNKPGNNGKN
                     GDSNPFGLSNKELAKMAAEQSAIRRKLEELRNELNKDGKGAGNQLNPLIKELEKQEEE
                     LVNKKYSDNLLKRQQDILTRLLESEKAIRERGFEEKRESNSGKNENYSNQIRFDEYNK
                     EKLKQIELLRTVDPVYNKYYKDKANQYFNLIK"
     gene            1490..1906
                     /locus_tag="PKN22_05015"
     CDS             1490..1906
                     /locus_tag="PKN22_05015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS42099.1"
                     /translation="MREGFTLINEITIPSSFDAMHDVESMIDTVCSKMEVNESFGNIL
                     IAVTEAVNNAILHGNKSQSTKQVKLKSADNQDSFCFIIEDEGNGFDFNSLPDPTAPEN
                     IEKENGRGIFLMRNLSDEVEFENNGSRVTIYFSKQL"
     gene            1903..2316
                     /gene="ybeY"
                     /locus_tag="PKN22_05020"
     CDS             1903..2316
                     /gene="ybeY"
                     /locus_tag="PKN22_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNS42100.1"
                     /translation="MIDINFEDIEPINEVNEEFLTQWLDKVTQLENKKLGELTFIFTS
                     DDYLLKVNQQYLEHDYYTDIITFDYCEDDYVIGDLFISIDRVKDNAQTNNTTFQNELN
                     RVIVHGVLHLCGYKDKSEVDEQLMRQKENQMLALI"
     gene            2427..4298
                     /gene="mnmG"
                     /locus_tag="PKN22_05025"
     CDS             2427..4298
                     /gene="mnmG"
                     /locus_tag="PKN22_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNS42101.1"
                     /translation="MLKDYDIIVVGAGHAGCEAANAAAKMGNSVLLVTMNMNTIAQMS
                     CNPAMGGVAKGQILREVDALGGGSGFVSDVSAIQFRMLNLSKGPAMWSPRTQNDRMLF
                     SEQWRLLLERNPNVDFWQDMCNGLLIEGTKVVGIKTQMGIPIKAKAVVLTNGTFLNGL
                     IYIGEKQFGGGRAAEKAATGITEELIQLGFEAGRMKTGTPPRVDGRSLDYSKMEIQYG
                     DENPGKFSFGNTKALTTQRPCHITYTNEQTHELLRTGFDRSPMFNGAIKSTGPRYCPS
                     IEDKINRFADRDRHQIFVEPEGWNTIEIYVNGFSTSLPEEVQLKAMHSIPGFENAKMF
                     RPGYAIEYDYFPPTQLKHTLETKLIENLYFAGQINGTTGYEEAACQGLMAGINAARKI
                     NEQSPLILSRNQAYIGVLIDDLITKGTKEPYRMFTSRAEYRILLRQDNADIRLTALAH
                     EIGMQDDAALKRVEEKVAGTESLKRVLRKEGVAPATINSFLQEKGSSEIDQQVKFSTL
                     LARPNISLSELLPFSKEAKELANELNEKNPDILPQTEIQMKYEGYIQREEDVAIKMNR
                     LENVKIPEAMDYNALKSISIESKEKLNKIRPATIGQASRISGVSPSDISVLLIALGR"
     gene            complement(4576..4887)
                     /locus_tag="PKN22_05030"
     CDS             complement(4576..4887)
                     /locus_tag="PKN22_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS42102.1"
                     /translation="MNKKATTNRLTSFSDHLDAQYGKRGTEIREQYEEEFEAFKLGAL
                     IQELRKEQGLTQEQLAEKCGTTKTYISRIENNASDIRLSTLMRIFRQGLGRNLKLNVS
                     H"
     gene            complement(4895..5221)
                     /locus_tag="PKN22_05035"
     CDS             complement(4895..5221)
                     /locus_tag="PKN22_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNS42103.1"
                     /translation="MRDKIVRQLFYYDNHYLDFFNTLKPDVQKKFNWTLQLIATLERV
                     PKKYFEHMTNTSGLYEIRVEVGSDIYRVFSFFDKGNLIILINGFQKKTQKTPKKEIEL
                     AEKIKK"
BASE COUNT         2029 a          799 c          976 g         1610 t
ORIGIN      
        1 aactaaaact atccaacgaa caaatgaata aacaattgga tagaagcatt gaaatgctaa
       61 aaaaaatgca agtagatgaa aaaattgatg ccattgaaaa tgaattaata aaattagctg
      121 aagatcaatt agcattaaaa aaagaaatag aaactaaaaa tttagataaa aacaaagcac
      181 aagaagaaca gtctaaaata aatgagcaat tcaatcaact aaaagaagat ttaaaaaaat
      241 tggaagagtt aaataaagaa ctcaatcgtc caatggaatt accttcatta gaaaaagaac
      301 aaaattcgac agaacaagag ctaaataatg cacaacaaaa tttaagtaaa ggcaagaata
      361 aaagcgcagg agaaaaccaa caaaatgctg ccgaacaaat gcaaaaaatg gctgatcaaa
      421 tggacgcagc gcaaaatgaa gctaacaaag aacaacaagg cgaagacatg gaaatgttgc
      481 gtcaaatttt aaaatcttta gttacactta gttttgagca agaacaatta atttctaatt
      541 tttcatctat aaaatcacaa gatccaaatt atagaaaata cggaagattg cagcgttcca
      601 ttatcgataa aacaattcca gtaaaagata gtttgtatga acttgctaag cgtcaaccca
      661 tgttatctga ttttgttgac aaagaattaa aaacaattca atataatcat aaacaagcat
      721 taaacaatat tcaagaacgt aatttacgac agttaaatgc taaccaacag tttgcaatga
      781 cttcttataa caatttagca ttaatgctca atgaatcatt gcaacaaatg caacaaaata
      841 tgcaagccat tatgaaaggt tctggaagtt gtagtaatcc tggtggtaaa ggaaaaccta
      901 gttctggtga aggacagctt ggaaatatga aagaacagtt gaaaaagcaa ttagaacaaa
      961 tgaaaaatgg ttctaatcca ggtggttcgg aaggaggtaa taaacctggt aataacggta
     1021 agaatggtga ttcaaatcct tttggtttat ccaacaaaga gcttgcaaaa atggctgctg
     1081 aacaaagtgc aatacgccgt aagttagaag aattgcgcaa tgagttaaat aaagatggaa
     1141 agggcgcagg taatcaactc aatcctttga ttaaagaatt agaaaaacaa gaagaagaat
     1201 tagtgaataa aaagtattca gataatttat taaaacgtca acaagatatt ttaacacgac
     1261 ttttagaatc tgaaaaagct attcgtgaaa gagggtttga agaaaaaaga gaatcaaatt
     1321 ctggaaaaaa tgaaaattat agtaaccaaa ttcgatttga tgagtataac aaagaaaaat
     1381 taaaacaaat tgaattattg agaacggttg atccagttta taataaatac tataaagata
     1441 aagccaatca atatttcaat ttaattaagt aatttctatg ttcgtaaata tgagagaagg
     1501 atttacacta attaatgaaa taactattcc atcttctttt gatgcaatgc acgatgttga
     1561 atcaatgatt gatacggttt gttctaaaat ggaagtcaac gaaagttttg gaaatatttt
     1621 aattgctgta acagaagctg tgaacaatgc tattttacat ggtaataaaa gtcagtcaac
     1681 gaaacaagtt aaattaaaat ctgccgacaa tcaagattct ttttgtttta ttattgaaga
     1741 cgaaggaaat ggttttgact ttaattcatt gccagatcca actgcaccag aaaacattga
     1801 aaaagaaaat ggacgcggta tttttttaat gcgtaatctt tctgatgaag ttgagtttga
     1861 aaataacgga agtcgcgtta ctatctattt ttctaaacaa ttatgataga tataaatttc
     1921 gaagatattg aaccaatcaa tgaagtaaat gaagagtttc ttacacagtg gttggataaa
     1981 gtaacacaat tagaaaataa aaaattaggt gaattaacat tcatctttac ttccgatgat
     2041 tatttattaa aagtaaatca acaatattta gagcacgatt attataccga tattatcacc
     2101 ttcgattatt gcgaagacga ttatgttatt ggggatttat ttatatcaat agatagagta
     2161 aaagataatg ctcaaactaa taacaccaca tttcaaaacg aactcaatcg cgttatagtt
     2221 catggtgttt tgcatttatg tggatataaa gataaatcag aagttgacga gcaattaatg
     2281 cgtcaaaaag aaaatcaaat gttagcttta atttaatgtt ccacgtgaaa cattcttttt
     2341 ttataaaacc ttttttattc tttgggtaga tttcagtaat tttgcaccct gttaaattgt
     2401 ttcacgtgaa acatcttttt tgaaaaatgt taaaagatta cgatataatt gttgttggtg
     2461 ccggtcatgc tggttgtgaa gctgcaaatg ctgcggcaaa aatgggaaat agtgtgttgt
     2521 tggtgacaat gaacatgaat accattgcac aaatgagttg taacccggca atgggaggag
     2581 ttgcaaaagg tcaaattttg cgtgaagtgg atgcattggg tgggggttct ggatttgttt
     2641 cggatgtaag cgcaattcag tttcgtatgt tgaacctttc aaaaggtccg gctatgtggt
     2701 cgccacgcac tcaaaatgat cgaatgcttt tttcagaaca atggcgtttg ttgttggagc
     2761 gtaatccgaa tgtggatttt tggcaagaca tgtgcaacgg tttgttgata gaaggaacaa
     2821 aagttgtggg tattaaaaca caaatgggaa ttccaatcaa agcaaaagcg gtggtgttaa
     2881 ccaacgggac gtttttaaat gggttgattt atattggtga aaaacaattt ggcggtggac
     2941 gagcagcaga aaaagcagca actggaatta cagaagaatt aattcaattg ggttttgagg
     3001 ccggaagaat gaaaacagga acgcccccgc gcgtagatgg tcgctcgctc gattactcaa
     3061 aaatggaaat tcaatacggt gatgagaatc cagggaaatt tagttttgga aacaccaaag
     3121 cgcttacaac tcaacgtccg tgtcatataa catataccaa cgaacaaaca cacgaattgt
     3181 tgagaacagg tttcgaccgc tcaccaatgt ttaatggtgc aattaaaagt acaggtccgc
     3241 gctattgtcc aagtatcgaa gataaaatta atcgatttgc agatagagat cgtcaccaaa
     3301 tttttgttga accagaagga tggaatacga tagaaattta tgtcaatggt tttagcacct
     3361 cgctaccaga agaagtgcag ttaaaagcaa tgcattctat tccaggtttt gaaaatgcta
     3421 aaatgtttcg accaggatat gcaatcgaat acgattactt tccgccaact cagttaaaac
     3481 atacattgga aacaaagttg attgaaaact tgtattttgc tggtcaaata aacggaacca
     3541 caggatacga agaagcagct tgccaaggat taatggcagg aatcaacgct gcaagaaaaa
     3601 tcaacgaaca atcacctttg attttaagtc gtaaccaagc ttacattggt gtgttgattg
     3661 atgatttaat aaccaaagga actaaagaac cttaccgcat gtttaccagc cgtgccgaat
     3721 accgtatatt gttgcgtcaa gacaatgccg atattcgttt aacagctttg gcacacgaaa
     3781 ttggaatgca agacgatgcg gctttgaaaa gagtggaaga gaaggtggcc ggaacagaat
     3841 cgcttaagcg cgtgttgcgc aaagaaggtg ttgcaccagc aaccatcaat tcgtttttac
     3901 aggaaaaagg aagcagtgaa attgatcaac aagtaaaatt cagcactttg ttggctcgac
     3961 caaatatttc gctttcagaa ttattgccgt ttagtaaaga agctaaagaa ttggcaaatg
     4021 aactgaatga gaaaaatcca gatattctgc ctcaaacaga aattcaaatg aaatacgaag
     4081 gctatattca acgtgaagaa gatgtggcta tcaaaatgaa tcgcttggaa aatgttaaaa
     4141 ttccagaagc aatggattac aatgccctca aaagtatttc catcgaatcg aaagaaaaat
     4201 taaataaaat tcgcccggca accatcggtc aagcgtctcg catttctggg gtaagtccaa
     4261 gcgatatttc ggttctttta atcgctttag gaagataatt tacgtaaaat attgataatc
     4321 aataaaatat ataattttta tagcttaaaa aacacgtcct ctctaatcga ttatctcgta
     4381 gtggtgaagg tgttttcctt tcagacgaga taaaagttgc ttaaaacgca aaaaaacaat
     4441 gtttcacgtg taaagacgta taatttttgg ttgtttaaat gaatttctac gtttgattaa
     4501 gatttatctg atgaattaaa aaatatatat ccaaaaaaga gtagttgtaa atggttggac
     4561 tattaatctc aaaaattaat gagatacgtt taattttaaa tttcttccga gtccttgtct
     4621 aaaaattctc atcaatgttg aaagtctaat atcactagca ttattttcaa tgcgggagat
     4681 gtatgttttt gttgttccac atttttcggc taactgttct tgagttaaac cttgttcttt
     4741 acgaagttct tgaatcaatg caccaagttt aaatgcttca aactcttctt cgtattgttc
     4801 tcttatttca gttccacgct taccatattg tgcatctaaa tgatctgaaa acgaggttaa
     4861 tctattagtt gttgcttttt tgttcatcga agtattattt tttaattttt tctgctaatt
     4921 ctatttcttt tttaggtgtc ttttgcgttt tcttttgaaa tccatttatc agaattataa
     4981 gatttccttt gtcaaaaaaa ctaaaaactc tgtaaatatc agaaccaact tctactctaa
     5041 tttcatatag cccagaagta tttgtcatgt gctcaaaata tttttttgga acacgttcta
     5101 aagtcgcaat taattgcaat gtccagttaa atttcttttg tacatcaggt tttaacgtat
     5161 taaaaaaatc aagataatga ttgtcataat aaaatagttg tcgaacaatc ttatctctca
     5221 tttataatta tagtttataa acacaaagtt agctaattag ataacataaa aacaaaaaat
     5281 tcctatcaaa attaaacaat atgaccaaaa tcatatattt tctaaaattt atggttgaac
     5341 tttgcagccg aattttataa acagttatcg tcatacttgc aatatggaga aacaaaagat
     5401 tgaactaatg gcgc
//
LOCUS       DAOQVQ010000120         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16744_length_5391_cov_3.206709, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000120 DAOQVQ010000000
VERSION     DAOQVQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16744_length_5391_cov_3.206709"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1835)
                     /locus_tag="PKN22_05040"
     CDS             complement(60..1835)
                     /locus_tag="PKN22_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS42104.1"
                     /translation="MKTFLLALLLLPFTLVAQQPIMSLKSQVDLMFIKKNAALIQKKN
                     KNMWQELPLIEINKIIYVSFVGKLKNNQVVSNSNDVIIGKGVGTIRSIRIRIDKLDKI
                     ANLPQISHLELAGKIKPDLYKVTYDTRVDSVHNGWGLAQGYSGKNVIIGVQDWGFDYT
                     HPMFYDTLLQNNRILAAWDQFKHAGPTPTGYNYGTEYATVNDLLTAECDTSNQYNHST
                     HGTHVSGIAGGSGAGVYSKGMAFENQFLFNTLIIDEAAAVDGWNWMYSKAQAAGKRLV
                     VNMSWGLYHFSGNDGTSLLSQAIEELTDLGVLFVSSAGNNGSVNFHFQKEFNNDSIRS
                     KVQFYDYALHDSMWGQSLHGWGTPGFDFDVKIQVYSTATNLVAETPYYSTSMNGYDEG
                     YLLISPTDTVWYNIVAQSAFPSNGKPMVRFRVKNKHAVNIVLVARANQGTVHFWNLVE
                     LTTNGGNWGQAFFTFGAWSIGGDAQYGIGEPTCATDCISVASHAAEFLHSSGNVFGGS
                     RSGFSSIGPRNDGAMKPDISAPGSSVVSSVNSYTTDSYSSLASTNFNSKTYHFTSLSG
                     TSMSSPAVAGICALIWDANPYLSSC"
     gene            1984..3315
                     /gene="rseP"
                     /locus_tag="PKN22_05045"
     CDS             1984..3315
                     /gene="rseP"
                     /locus_tag="PKN22_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009420863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNS42105.1"
                     /translation="MEVLIIVSQLILSLAILVVLHEFGHYIAARMFDIRVEKFYLFFD
                     PWFSLFKRKIGDTEWGIGWLPLGGYVKISGMIDESMDKEQMAKPPQPWEFRSKPAWQR
                     LIVMIGGVTVNFFLALLIYAMILFVWGKQYYTVDSAKKYGMSVAYPELKKYGLEDGDI
                     PVKVGEQKVDNIAKMFSAIVIEGERKLTVNRNGETEELELPQDFDQVALKNSYTSLYN
                     LRIPTVVYKVVEKSNAAKGGLKEKDSIVSINGDATPYFNDVTEVLAANKSKSITMEVM
                     RNNALTQLTLNVDSTGKVGFQPQDYNYFNIPYATEKFGFFESFPAGVTLGVETLSSYI
                     KSMGLIFTKEGAKQVGGFGSMSKIFAPVWDWKVFWSATAFLSIVLAFMNILPIPALDG
                     GHVVFLLYEMVTGKEAPQKVLEIAQYIGFFLLIALMLYANGNDLFKFLTGK"
     gene            complement(3365..4006)
                     /locus_tag="PKN22_05050"
     CDS             complement(3365..4006)
                     /locus_tag="PKN22_05050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS42106.1"
                     /translation="MEFISEELDKYVCAHTEIEPPYLQELNRKTHLEVLQPRMLSGHF
                     QGRVLSLLSHMIQPQRILEIGTYTGYSALCLAEGLTPTGQLITIDVNEELEELVASFI
                     EKSGRAQQIKPMIGDAMQIIPTLDEMFDIVFIDADKKNYINYYNLVFDKVKPGGYIIA
                     DNVLWSGKVLEDYEKLDKSTQLLMDYNKMVHQDERVQEVLFPIRDGLMIARKK"
     gene            complement(4051..4149)
                     /gene="ffs"
                     /locus_tag="PKN22_05055"
     ncRNA           complement(4051..4149)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN22_05055"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4288..5328
                     /locus_tag="PKN22_05060"
     CDS             4288..5328
                     /locus_tag="PKN22_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNS42107.1"
                     /translation="MYKARLITHEGKRRIAVEFDNKPELIQRFKLLSGATWSRTLKCW
                     HLPDTKEYRKLFKISVFENKHLPHRFYPLLERFVKWLRSKRYSESTIKTYSEVVAIFF
                     AFHHDKQVDEITNNDVVVFNNEYILKRKLSSTYQNQTVNALKLFYRVVENRNIDVESI
                     HRPRVEHRLPHVLSMEEVKQLLQALPNVKHRVMLSLIYACGLRRSELLNLRPTDIDSK
                     RMTIFIQQSKGKKDRLVPLTEKILHLLREYYILYRPKVWLFEGQEACSQYSPKSLQNV
                     MKMALKKSNINKPATLHWLRHSYATHLLDKGTDLRYIQELLGHNSSRTTEIYTHVSTR
                     DLQRIRSPFDDL"
BASE COUNT         1705 a          967 c         1019 g         1700 t
ORIGIN      
        1 gctggtattt gacctgtata ttgatccaaa cgagctgttt ctatgatgat ttatttcact
       61 taacacgatg ataaataggg atttgcgtcc caaattaaag cgcaaattcc agcaacagcg
      121 ggtgacgaca tagaagtgcc cgacaatgaa gtgaaatggt atgttttact attgaaattg
      181 gttgaagcaa gtgagctata actatcggta gtatatgaat taacactact aacgacagaa
      241 gaacctggag cagaaatatc gggtttcatt gcaccatcat ttcttggtcc aatgcttgaa
      301 aatcctgaac ggctacctcc aaaaacatta ccactagagt gcaaaaattc agcagcatga
      361 gaagctacag aaatgcaatc agttgcacaa gttggttcac caataccata ttgagcatca
      421 cctccaatag accaggctcc aaatgtaaaa aatgcttgtc cccaatttcc tccattggtt
      481 gttaattcca ctaaattcca aaaatgcacg gttccttggt ttgcacgggc aactaacaca
      541 atatttacag catgtttatt tttaacgcga aaacgcacca ttggttttcc gttggatggg
      601 aaagctgatt gagccacaat gttataccaa acagtgtcgg ttggagaaat caataaatat
      661 ccttcatcat atccgttcat tgatgttgaa taatagggag tttctgcaac aaggttggtt
      721 gctgtggaat agacctgaat ttttacatca aaatcaaatc cgggtgtccc ccaaccatgc
      781 aaactttgtc cccacattga atcgtgaaga gcataatcat aaaattgcac ttttgaacgt
      841 atgctatcgt tattaaattc tttttgaaaa tgaaaattaa cactgccatt attgcctgct
      901 gatgaaacaa ataaaacccc taaatcggtc agttcttcaa tagcttggct taataaggat
      961 gttccatcat taccactaaa atgatacaag ccccagctca tatttacaac caagcgtttt
     1021 ccagccgcct gagctttaga atacatccag ttccagccat caactgcagc cgcttcgtca
     1081 ataatcaacg tattgaataa aaattgattt tcaaaagcca ttcctttaga gtaaacacct
     1141 gctccacttc cgccagctat gcctgaaacg tgcgttccgt gagtagagtg attgtattga
     1201 ttagaggtgt cgcattctgc agttaataaa tcatttacag ttgcatattc agttccataa
     1261 ttataacctg taggtgtggg tccagcgtgt ttaaattgat cccaagcagc caaaatgcgg
     1321 ttgttttgca aaagtgtatc ataaaacatc ggatgggtgt aatcaaaacc ccaatcttgc
     1381 actccaataa tcacattttt cccagaatat ccttgtgcca atccccaacc attatgaaca
     1441 ctatcaacgc gcgtatcgta ggtaacttta tacaaatctg gttttatttt accggccaat
     1501 tctaagtgcg aaatttgtgg taaattggct attttatcca atttatcaat tcggatacga
     1561 atacttctta tagttccaac tcctttgcca ataataacat cattggaatt agaaacaact
     1621 tggttatttt ttaatttacc aacaaatgaa acataaatta ttttgtttat ttctatcaat
     1681 gggagttctt gccacatatt tttgttcttt ttttgaatca atgctgcatt ttttttaatg
     1741 aacattaaat caacttgaga tttcaacgac atgatgggct gttgcgccac taaagtgaat
     1801 ggaagtagca atagagcaag taagaatgtt ttcattttca ttttttttaa ttgatgtaaa
     1861 gataacaaaa gttaatattt attaataaaa tgtatgaaat catttgttta tagcaatgcg
     1921 gtgtggatga aatactaaac attaattata tttgttggct taaattaaaa tgataaacac
     1981 acaatggaag tattgataat tgtttcacaa ttgattttat cgctcgcaat tttagttgta
     2041 cttcatgaat ttggacacta catagcagcc agaatgtttg atattcgagt tgaaaaattc
     2101 tatttatttt ttgatccttg gttttcattg ttcaaacgca aaattggtga taccgaatgg
     2161 ggaattggtt ggttaccatt aggaggatac gtcaaaattt cgggaatgat tgacgaatcg
     2221 atggataagg agcaaatggc aaaaccacca caaccatggg aatttcgttc aaaacctgcg
     2281 tggcaacgtt taattgtgat gattggtggt gtaacggtta acttcttttt ggcactttta
     2341 atttatgcca tgattttatt tgtgtgggga aaacaatact acacggttga ttctgctaag
     2401 aaatatggta tgtcagttgc ctatccagaa ctcaaaaaat atggtttaga agacggcgat
     2461 attcctgtaa aagttggtga acaaaaagtg gataacattg ctaaaatgtt ctctgccatt
     2521 gttattgaag gcgaaagaaa attgaccgtt aatagaaacg gagaaacaga ggaattggaa
     2581 ttaccacaag attttgatca agtagcattg aaaaacagtt acaccagttt gtacaatttg
     2641 cgaataccta ctgttgtgta taaagtagta gaaaaatcaa acgctgcaaa aggtggtttg
     2701 aaagaaaaag acagcattgt atcaatcaac ggagatgcaa ctccttattt taatgatgtt
     2761 acggaagtgc ttgctgccaa taaaagcaaa agtataacaa tggaagtaat gcgtaataat
     2821 gcattgacac aactcacatt aaatgttgat tctactggta aagttggatt tcaaccgcaa
     2881 gattacaatt atttcaacat tccttatgca acagaaaaat ttggattttt tgaatctttt
     2941 ccagcaggtg taactttggg tgttgaaacc ttgagtagtt atataaaatc catgggatta
     3001 attttcacca aggaaggagc aaaacaagtg ggcggatttg gttctatgag taaaattttt
     3061 gcgccagttt gggattggaa agtattttgg tcggcaactg catttttatc cattgttttg
     3121 gcatttatga acatattacc tatcccagcg ttggatggtg gacacgtggt tttcttattg
     3181 tatgaaatgg taacaggaaa agaagcacct caaaaagtat tggaaattgc gcaatacatt
     3241 ggtttctttt tattgattgc attgatgctc tatgccaacg gaaacgattt attcaagttc
     3301 ctcactggca aataaaattc ctaaaaaatt catgaagtag gcgttttttt ttattgaaga
     3361 acgcctattt ttttcgagca atcatcaacc catcgcgaat gggaaacaaa acttcttgca
     3421 cacgctcatc ttggtgcacc attttattat agtccatcaa caattgagta gatttatcta
     3481 atttttcgta atcttccagc acttttccag accacaacac attgtcggca ataatgtagc
     3541 cacctggttt tactttgtca aatactaaat tgtagtaatt gatgtaattt tttttgtcgg
     3601 catcaataaa aacaatatca aacatttcgt ctagcgttgg tataatttgc atggcatcgc
     3661 ctatcattgg ttttatttgc tgtgctctcc ccgatttttc aataaacgaa gcaaccaact
     3721 cttccaattc ttcattcaca tcaatggtga ttaattgtcc tgttggtgtt aaaccttctg
     3781 ccaagcacaa tgccgaatag ccggtatagg ttccaatttc taaaattcgt tgtggctgaa
     3841 tcatgtgcga aagcaaactc aaaacacgtc cttgaaaatg gccgctcaac atgcgtggtt
     3901 gcagcacttc taaatgagtt ttacggttta attcttgcaa atacggcggt tcaatttctg
     3961 tgtgtgcaca tacgtatttg tcgagttcct cactaataaa ttccatgttt gattgttttt
     4021 cctgcaaaag tagtaaaaaa aggaaaaaaa gaggggcaag ctacttacat cgcaccgcta
     4081 caaccttcgg cccttgctac attcctgtcc taggggatta gaagggagct ggtcgtgcaa
     4141 gacttacccc ggcgcaaaag tagtgatttt ttcttatttg aaaagcgttt cctaaaattc
     4201 ttttgggata ttttcaaact ttttttctaa aaatttggtt taaacgattc ttttttatta
     4261 atttagaata aaccaaatat accttttatg tacaaagcta gattaataac ccacgagggg
     4321 aaacggcgta tagctgtaga atttgacaac aaaccggagc ttattcagcg gtttaaatta
     4381 ctatcgggcg ctacttggag tcgaacatta aaatgttggc atttgcccga caccaaagaa
     4441 tatcggaagc tttttaaaat ttctgttttt gagaataaac atttgccgca tcggttttat
     4501 ccgttgttgg agcgttttgt gaaatggttg cgatctaagc gttatagtga aagcacgata
     4561 aagacttatt cggaggttgt ggcaatattt tttgcttttc atcatgacaa gcaagtggat
     4621 gaaattacca ataatgatgt ggtggtattt aacaatgaat acattctcaa gcgcaagtta
     4681 tcgtccacct atcaaaatca aaccgtcaat gcgttgaagt tattttatcg tgttgtagag
     4741 aaccgcaaca ttgatgtaga aagtattcac cgaccacgag tggaacaccg actacctcat
     4801 gttttgagta tggaagaggt gaaacaactt ttgcaagctt tgcccaacgt taagcatcgg
     4861 gtgatgttga gtttaatata tgcatgtgga ttgcgcagaa gtgagttatt gaatttacgt
     4921 ccgaccgaca ttgacagcaa gcgcatgacc atttttattc agcaatcgaa gggtaaaaaa
     4981 gaccgtttag ttccactaac cgagaagatt ttgcatttat tgcgcgagta ttacattttg
     5041 tatcgtccga aagtatggtt atttgagggt caggaggcgt gttcacagta ttcgcccaaa
     5101 agtttacaaa atgttatgaa gatggcgttg aaaaagtcaa acatcaacaa gccagccacc
     5161 ttgcattggt tgcgtcacag ctatgctaca catttattgg acaagggcac tgacttgcgt
     5221 tacattcagg agttattggg gcacaacagt agccgcacaa ccgaaattta cacgcatgtc
     5281 agcactcgcg atttacaaag aatccgcagt ccgtttgatg atttatgaca aacatatttt
     5341 gaaacaagta aagctatatg atttaaaatt gccatgaata atttaagaaa a
//
LOCUS       DAOQVQ010000121         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_16958_length_5339_cov_3.614875, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000121 DAOQVQ010000000
VERSION     DAOQVQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16958_length_5339_cov_3.614875"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKN22_05065"
     CDS             complement(<1..763)
                     /locus_tag="PKN22_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS42108.1"
                     /translation="MEKKWIIKSPIDHTTVEKFSSSIKTTPLIAELLLQRGITTRDEA
                     ELFFRPKLEQLHDPFLMQGMQEAVNRLDKALEQQEKILLFGDYDVDGTTAVAMMYSFL
                     KNHTPQLDFYIPDRYQEGYGLSEKGIRFAHENGYHLMILLDCGIKSVELIELGKELGI
                     DFIICDHHEPGEVVPATIVLDPKQPDCSYPYKELCGCGVGFKLLQALSIQKKWELQHL
                     YILLNFVAVAIGADIVAITGENRILAHHGMRLLNEH"
     gene            823..1500
                     /locus_tag="PKN22_05070"
     CDS             823..1500
                     /locus_tag="PKN22_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42109.1"
                     /translation="MKKYPFVATLFQLFFCLFVFFPATLAQKSFEFANILPVEAQPIT
                     SVAPQFFGNYESDKGKEFTYEFNDKGIFLKTINIQAISQKTIRETGKYKVQNGYLFGV
                     NEDSIPCFFEDSNYYFGVRNSIQLVGADIANQLCLNSPTSYILNSKSEHGFVPMLLEF
                     KNNQLIISSFDYDAEGTIFKKIKDQQQLVLENNPLSIYLLQPTLKEWQKTAKKEIFRN
                     PRVFIKQ"
     gene            1911..3557
                     /locus_tag="PKN22_05075"
     CDS             1911..3557
                     /locus_tag="PKN22_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42110.1"
                     /translation="MSQKKYTLKFINNEYKVIKKHPNQQFKDSISLKAYLKEFQLFAQ
                     KKGYLLASIDSLYVENKTNYTVYFTSGEKFTTAQLKIAPEEQRFIRKKAGLSEKIIAK
                     TPLTPQEFSATLTTIRDAYLNNGYPFAKIQLDNVEINATSVSGDVVVDRGKQLKWIDL
                     HVKGDSSISKKFIGSLIGIKVKALYSESDFFKISQRLKQINYFEEIKPAELLFTKDGV
                     ELFTYLKTKKLSNVNGIIGLQPNATTGKLALTGELSLKLQNILKRGEMLYIDWRSIQA
                     QTQSLRSQLTYPYFLNTNFGLDGTFNLYKRDSSFLEINGTAGVNYYLNGGSYIKAFYE
                     NHTSNLLSGAQNSTLTNLGKVKSNSYGLAFVYNHVDYLQNPTRGLIIDTKISIGSRKS
                     QITDSSEQVKSTVYGGKLAISGFIPLAKRHVLMLASRSYFYNAPIIFINEAYRFGGLT
                     TQRGFNEEELYATTQTTLAVEYRFLVDKNSFAFAFFDQSWYENNAQKYYNDALFGFGL
                     GFSFGTKIGIFSISYALGKQFNNPIQFKNSKVHFGYIAYF"
     gene            3609..4733
                     /locus_tag="PKN22_05080"
     CDS             3609..4733
                     /locus_tag="PKN22_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNS42111.1"
                     /translation="MQTQVETLKVEKKPRIVFIDMARSIAILLMLEGHFVDDSLMPIY
                     RDLNNPIYATWLFIRGFTGPIFLTVTGIVFVYLLLGKRDEPYFQNIRIKKGFKRVVEL
                     LFWGYILQYYAFHVLQCIGVGIFTILLIFGIYKIIKIIPLWIYYFIAGTILFGLNLYF
                     DQLPLYEVWPKNGPYFIQNMFHGPANRAIYPLVPWLGYTMYGAMLGCLLHDLKKHVKT
                     WYAPVVTGAIGATLFLFPKELLEGLDALIGSPFTLNLGILDWLYMRFGMVLLVLSVLM
                     AIEKMIGDVKPNLFIKVGQNTLTIYIVHMILLYGSITGLGLNRIFRHNLGPWQVTIAA
                     ALFIVFFLVFIKYLDQIKAKLEFILGPIRRFFNKLYNLPG"
     gene            complement(4771..>5339)
                     /locus_tag="PKN22_05085"
     CDS             complement(4771..>5339)
                     /locus_tag="PKN22_05085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42112.1"
                     /translation="NLAIVPVGNVWKEIRKNYPEYNLYQKDQHHPTKIGSYIVASSFY
                     SAIFRSSPEGGYTAGLDDKMALNIQRTAYSYVKDHLSEYNLDSNIVEVSYKFTNEGKY
                     EAVCYANYPEATSIKWEFGDNTADSTHARVVHHYKTFGNYKVKLTVQDACGERIIYKN
                     VHFKEPYKPKAPTTSQPKTKDTSIKKRI"
BASE COUNT         1709 a          948 c          931 g         1751 t
ORIGIN      
        1 gatgttcgtt caataagcgc attccgtgat gggctaaaat tctattttca cctgtaattg
       61 caacaatatc ggcacctatg gcaacagcta caaaattcaa taggatgtac aaatgttgta
      121 attcccattt tttttgaata gaaagtgctt gcaacagttt gaaaccaact ccacaaccgc
      181 acaattcttt gtaaggatag gaacaatcgg gttgtttggg gtcgagcaca atggtggctg
      241 gaacaacctc tcccggttca tgatgatcac aaataatgaa atcaattccc agttctttac
      301 ccaattctat taattcaacc gatttaatgc cacaatccaa taaaatcatc aagtgatagc
      361 cgttttcatg ggcaaatcga atgccttttt cactcaatcc ataaccttct tggtaacgat
      421 cggggatgta aaaatccaac tgaggagtgt gattttttaa aaagctatac atcattgcta
      481 ccgccgtagt gccatccaca tcgtaatcgc caaaaagcag aattttttct tgttgttcca
      541 atgctttatc caaacgattg acagcctctt gcatgccttg catcagaaaa ggatcgtgca
      601 attgctctag tttgggtcta aaaaaaagtt cagcttcatc acgggttgta atgcctcgtt
      661 gaagcaataa ttcagcaatg agaggcgttg tttttattga tgaactaaat ttttctacag
      721 ttgtatgatc aattggtgat ttaattatcc actttttttc catttagttg gtaaatttgt
      781 aattattaaa aatatcgaat acgcgattta tacttttaaa ttatgaaaaa atatccgttt
      841 gttgcaacac ttttccaatt atttttctgc ctttttgttt tttttccagc aacattggct
      901 caaaaatcat ttgaatttgc aaatatctta cccgttgaag ctcaaccaat aacgagtgtt
      961 gctcctcaat tttttggcaa ctatgaaagc gacaaaggta aggagtttac ttacgaattt
     1021 aacgataaag gcattttttt gaaaaccatc aacattcagg caatttcaca aaaaaccatt
     1081 cgtgaaactg gaaaatataa agttcaaaac ggttatcttt ttggagtgaa tgaagactcc
     1141 atcccttgtt tttttgaaga cagcaactat tattttggag tgcgcaacag catacagttg
     1201 gtgggtgcag atattgcgaa tcaattgtgt ttaaattcgc caacaagcta tattttgaat
     1261 tcgaaatcag aacatggctt tgtaccgatg ttgttggaat ttaaaaacaa ccaattaatc
     1321 atttcatcgt ttgattacga tgccgaagga acaattttca aaaaaataaa agaccaacag
     1381 caactggttc ttgaaaacaa tccgctttca atatatttgc tgcaaccaac gctcaaagaa
     1441 tggcaaaaaa cggcgaaaaa agagattttt agaaacccac gtgtttttat caaacaataa
     1501 aagcgagttg aataaccgtt gaccacaaaa aaaaggctac tattcagcag cctttaatac
     1561 attttaatgc ttattaaaaa ttagcgagaa gctaaatctt cagctggtgc aacttgtaca
     1621 cttccgtatg catcacagtg cccaccacgt gaagaaccac aagatgttgc taccatagaa
     1681 acaccaaagc taataagcat aaaaaacaaa aattttttca tctgttgttg tattaaaaaa
     1741 ttaaagtcta aagattgaac gataacaaat ttactaaaaa aactattatt agcacaatat
     1801 tattttcatc tttccattaa ttctagaata aataacgtaa ttttcaaccg aatattttat
     1861 taatgcgttt ttgttttttc gttatattat tgctgtttcc gttcatttcg atgagccaga
     1921 aaaaatacac tttaaaattt attaacaatg aatataaagt tataaaaaag caccccaatc
     1981 aacaattcaa agattcaatt tcgctcaaag cctatttaaa agaatttcaa ctatttgctc
     2041 agaaaaaagg ctatctttta gcttcaatag actctcttta tgttgaaaat aaaaccaatt
     2101 acaccgttta ttttacaagt ggcgagaaat tcacaacggc tcaactcaaa attgctcctg
     2161 aagaacaacg gtttatacgc aaaaaagcag gtctatccga aaaaatcatc gctaaaacac
     2221 cactaacacc tcaagaattt tcggctacac tcaccaccat tcgtgatgct tatttgaaca
     2281 acggctatcc atttgcaaaa atacagttag ataatgtgga aattaatgct acttcggtga
     2341 gcggtgatgt ggtggttgac cgtggcaagc agttgaaatg gattgattta catgtaaaag
     2401 gggattcctc catttctaaa aaatttattg gaagcttaat tggtataaaa gttaaagcgt
     2461 tgtacagtga aagtgatttc tttaagattt cacagcgctt aaaacaaatc aattattttg
     2521 aagaaataaa accagccgaa ttgttgttta ccaaagatgg agtggagttg ttcacctatt
     2581 taaaaaccaa aaaattaagt aatgtaaatg gtattattgg tttgcaaccc aatgcaacaa
     2641 cagggaaatt agcactcact ggggagttga gcctcaaatt gcaaaatatt ttgaaacggg
     2701 gagaaatgtt atacattgat tggcgcagca tacaagctca aactcaatcg ttacgttcac
     2761 aattgacata cccctatttt ctcaatacca attttggact tgatggcacg tttaacctct
     2821 acaaacgcga ctcttctttt ctagaaatta acggaacagc aggagttaat tactacctca
     2881 atggaggcag ctatatcaaa gcattttatg aaaatcatac ctccaattta ttgtcgggtg
     2941 cacaaaattc aacacttacc aatttaggaa aagtaaaatc caactcctat ggattggcat
     3001 ttgtatacaa tcacgttgat tatttgcaaa atccaacacg tgggttaatc atagatacca
     3061 aaatatccat cgggtctaga aaatcacaaa tcaccgattc aagtgagcaa gtaaaaagca
     3121 cggtttatgg tgggaaatta gccatcagtg ggtttattcc cttagcaaaa cgccatgtgc
     3181 tgatgcttgc tagtcgctct tatttttaca atgcaccaat tatttttatc aacgaagcat
     3241 atcgttttgg tggtttgact acacaacggg gatttaacga agaagaattg tatgccacca
     3301 cccaaaccac attggcggta gaataccgtt ttttggtaga taaaaactca tttgcctttg
     3361 ctttttttga ccaaagttgg tatgaaaaca atgctcaaaa atattacaat gatgcattat
     3421 ttggttttgg attaggcttt tcgtttggaa caaaaattgg gattttttcc atttcctatg
     3481 cactaggaaa gcaatttaac aatcctattc aattcaaaaa cagcaaggta cactttggat
     3541 acattgctta tttttaagtg ttttttctat taaaaaatgt ttgtacattt gcctttctta
     3601 aataaaacat gcaaacgcaa gtagaaacgc taaaagtaga aaaaaaacca cgtatcgttt
     3661 ttatcgatat ggcgcgatca attgccattc tattaatgtt ggaggggcat tttgtggatg
     3721 attcacttat gcccatttac cgcgacttaa acaatccaat ttatgctact tggcttttta
     3781 tcagaggttt tactggacca attttcttaa cggttacagg aatcgtgttt gtgtatttgc
     3841 tactcggaaa aagagatgag ccttattttc agaatattcg tatcaaaaaa ggattcaaac
     3901 gagtagtcga actgttgttt tggggatata ttcttcaata ctatgctttc cacgtgttgc
     3961 aatgcatagg agttggtatt tttactattc ttttaatttt tggaatttat aaaataataa
     4021 aaatcattcc gttatggatt tattatttta ttgcaggaac catcttgttt ggtcttaact
     4081 tatatttcga ccaattaccg ttgtatgaag tatggccaaa gaacggacct tattttatac
     4141 aaaacatgtt tcatggtcct gccaacagag caatttaccc actcgttcca tggttgggct
     4201 ataccatgta tggagccatg ttgggttgct tgttgcacga tttaaaaaag catgtaaaaa
     4261 cgtggtatgc cccagttgta acaggtgcaa tcggagcaac acttttcctt ttccctaaag
     4321 aattgttaga aggtttggat gcattgattg gaagtccgtt tacgcttaac cttggaattc
     4381 ttgattggtt gtatatgcgc tttggtatgg tgttgttggt gttgagtgtt ttaatggcta
     4441 tcgaaaaaat gataggtgat gtaaaaccta atttatttat taaagtagga caaaacacct
     4501 tgaccatcta catcgtgcac atgattcttt tgtatggtag tatcacaggt ttgggcttga
     4561 accgaatttt tcgacataat ttaggtccgt ggcaagtaac cattgctgct gctttgttta
     4621 ttgtgttttt cttagttttt ataaaatatt tggatcaaat caaggcaaaa ctggaattta
     4681 ttttaggtcc aattcgccgc ttctttaata aactgtataa tttaccaggg tagttactaa
     4741 ccaccaattt tcatggtaat cagaggaata tcaaatccgt tttttgattg atgtatcttt
     4801 cgttttgggc tgcgatgtag tgggagcctt gggtttgtag ggctctttaa aatgaacatt
     4861 tttatagata attcgttctc cacaagcatc ttgaacggtt aattttactt tgtaattacc
     4921 aaaggttttg taatggtgca caacacgtgc atgggtacta tcggctgtgt tatcgccaaa
     4981 ttcccatttt atagatgttg cctctggata attggcatag cacaccgctt cgtatttgcc
     5041 ctcattggta aatttatagc tcacctccac aatattgctg tctaaattat attcagaaag
     5101 atggtctttc acatacgaat aagccgttcg ttgaatatta agcgccattt tatcgtccaa
     5161 tccagcggtg tatcctccct ctggcgatga acggaaaatt gccgagtaaa acgatgatgc
     5221 aacgatataa gatccaattt ttgtaggatg gtgctgatct ttttggtata aattgtattc
     5281 tggataattt ttgcgtattt ctttccatac attgcccact ggaactatag ccaagttgt
//
LOCUS       DAOQVQ010000122         5328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17006_length_5328_cov_3.143941, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000122 DAOQVQ010000000
VERSION     DAOQVQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17006_length_5328_cov_3.143941"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKN22_05090"
     CDS             <1..447
                     /locus_tag="PKN22_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS42113.1"
                     /translation="IAKEGDVIGFRSLFSEEPYRLSATTLEDCSVCVIKTDDFSKQVE
                     NQPELLQAVLRELSKEAGELALFIKTMAQKTVRQRLAIILLVLNEIYKNQQINLSRED
                     LANFVGTATETVIRLLKDFKEEGVIEIHGRAIELLNVPLLVQEAER"
     gene            complement(444..2813)
                     /locus_tag="PKN22_05095"
     CDS             complement(444..2813)
                     /locus_tag="PKN22_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNS42114.1"
                     /translation="MEKNLSCDHCGDEIIGNQHYTYKEKSFCCNGCMTVYQLLTDNQL
                     GNFYQLEEKSGVRPQKIQQHKYDFLEVPEIKARFIDFEDDTFVKITLFLPEIHCSSCI
                     YLLENLPKILPAVTSCQVNFVQREALITYRKNELQLSELAHFLNKIGYAPNFGNRKEQ
                     SKKIDKVFFYKLGIAAFAFGSIMLWSFPEYLGIDTMDYKIRFFTSILSLLVSVPVLLF
                     SARDYFISAYKSIKSKSLNLDVPIAIGIIALYSQSVFTIITEGGPGYMDSFAGFIFFL
                     LIGKWFQNRTYKSLSFERDYTSYFPVAVTKITDNGEEIVEIEKINVGDKILIRNEEII
                     PCDSILQSETAKIDYSFVTGESVPIYKSKGDYIYAGGKLLGQKVVLKSDRPSNRSLLT
                     QLWNDVKFDKLKPNRYKFQDTIARYFLWVVLAIALLSAIAWVFIDYTRILEIVVAVLI
                     VACPCALALSSPFTFGNSMKLMGRKGLYLKNTAVIERMNEITDVVFDKTGTLTESTNL
                     QVEYIGDELSDSEKELLCSLANCSTHPLSRAIVRYYKLQGIHSTATISNFVEEVNKGI
                     SCTSSENLSASIGSHVYLFGNTVSSNETETYFKIGEKSGKFVFHSELRLGVHSLIKSL
                     NNYTVHVLSGDKDKDYELIKNLVGGDSTVRFNQTPNDKLDYVLNLQQEGKKVLMVGDG
                     LNDSGALGVAHVGIAVSEDVFRFTPSSDAIIKADKLARLNQFLEMSHYAKKVLKICLG
                     FSLVYNTIGLSFAVTGALSPLIAAIIMPMSSITIVALSTLLVHLKTFKN"
     gene            2996..3154
                     /gene="rpmH"
                     /locus_tag="PKN22_05100"
     CDS             2996..3154
                     /gene="rpmH"
                     /locus_tag="PKN22_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNS42115.1"
                     /translation="MKRTFQPSVRKRRNKHGFRSRMATKNGRRVLAARRRKGRKKLTV
                     SDEPMHKR"
     gene            complement(3401..3709)
                     /locus_tag="PKN22_05105"
     CDS             complement(3401..3709)
                     /locus_tag="PKN22_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42116.1"
                     /translation="MKNNKLKFALIFVICLLIVNIPLWFFPIQLFPGVIEFQNGLQAV
                     TIEAPLSLSHFIGKGFNEGDLENVNDFYLKPTGYALAFIICIGLPFIIALRWSNQQKK
                     "
     gene            3803..>5328
                     /locus_tag="PKN22_05110"
     CDS             3803..>5328
                     /locus_tag="PKN22_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS42117.1"
                     /translation="MNLYTNKQKWKIVLLIVALLLVGASLFVSNEIVSKVAAQERQRA
                     KQWASMIKKRVELIQLTDRTFTQLREKEKEKMKLWVDASKEIANPSAYSIPEFPMQIV
                     KDNNDIPVILLDNDANISGYRNLDFDTSNIRQVYPELSSKKLIKKFDDSLRVLAGVWE
                     KKNPAFTVEVYQGLFMKYVYDDSKEISRLEKDRDSLFKSFNKEIIEDEELVPVMLINQ
                     ETRQVIGTNINDSVFMHTDVNSLIRDMSKTNDSIVIDFSNNVKNVLYFDSSPVVKQLQ
                     YFPYIQFVIIGLFVFIAYLLFSTFRKAEQNQVWAGMAKETAHQLGTPISSLMAWVELL
                     RSQEVDAMIVDEMHKDIDRLNKVTDRFSKIGSEAKMESMNLSVTVQEIIDYLQVRTSK
                     QVQFTSKIEENVMAPHSSSLISWVIENIAKNAIDAMEGKGKLNITLKSTSEMAVIYIS
                     DTGKGMTPQQIRSIFKPGYTTKKRGWGLGLTLVKRIVEEYHKGKVVVEKSQIGEGTTF
                     "
BASE COUNT         1822 a          920 c          940 g         1646 t
ORIGIN      
        1 attgccaaag agggcgatgt gattgggttt cgatccttgt tcagcgaaga gccttatcgt
       61 ctttctgcaa caacacttga agattgtagt gtatgcgtta ttaaaacgga tgacttttca
      121 aaacaagttg aaaatcaacc tgaattattg caagctgtat tgcgtgaatt atccaaagaa
      181 gctggtgaat tagctttgtt tatcaaaacc atggcacaaa aaactgtgcg tcaacgtttg
      241 gcaataattt tattggtctt gaatgaaatt tataaaaatc agcaaattaa tttatcgcgt
      301 gaagatttag caaattttgt tggaacagct acagaaacag tcatacgcct tcttaaagat
      361 tttaaagaag aaggtgttat agaaattcac ggtcgtgcta ttgaattatt aaatgtgcca
      421 ctactggtgc aagaagcaga acgttaattt ttaaaagttt ttaggtgcac caataatgta
      481 cttaatgcta caatagtaat agaactcatt ggcattatga tggctgcaat taagggtgaa
      541 agtgctccag tgactgcaaa cgataaacca atggtgttgt aaaccaacga aaaccccaag
      601 caaattttta atactttctt agcatagtga ctcatttcta aaaattgatt caatcgcgct
      661 aatttatcag ctttgataat tgcatccgac gatggtgtaa aacgaaaaac atcttcagaa
      721 actgcaatgc caacgtgtgc aacgcccaat gctcccgaat cgttcaatcc gtcaccaacc
      781 atcaatactt ttttgccttc ttgttgcaaa ttcaacacat aatccaattt atcgttgggc
      841 gtttgattga agcgaacagt gctatctcca cccaccaaat tttttatcaa ttcataatct
      901 ttgtctttat cgcccgataa aacatgtacc gtgtagttgt ttaaactttt gatcaacgaa
      961 tgtacgccaa gacgcagttc ggagtggaac acaaatttcc cagatttttc accaattttg
     1021 aaatacgttt ctgtttcatt gctgctcact gtgtttccaa ataaataaac atgacttcca
     1081 atgcttgctg ataaattttc tgaagatgta caagaaatac ctttatttac ttcttccaca
     1141 aaattggaaa ttgttgctgt tgaatgaata ccttgcaatt tgtaatagcg aacaatggcg
     1201 cgagaaagtg ggtgagtgga acaattggcc aacgaacaca ataactcttt ttcactgtcc
     1261 gaaagttcat caccaatata ttcaacttgt aaattagtac tttcggtcaa cgttcctgtt
     1321 ttgtcaaaca caacatcggt aatttcattc atacgttcaa taacagccgt atttttcaaa
     1381 tacaaacctt tacgtcccat caatttcatg ctgtttccaa atgtaaatgg ggaagaaagt
     1441 gccaatgcac acggacaagc aacaatcaac acagcaacaa caatttctaa tattcgcgtg
     1501 taatcaataa atacccaggc aattgcagaa agcaatgcaa tggctaaaac tacccacaaa
     1561 aaataacggg caatggtatc ttgaaattta tagcgattgg gctttaattt atcaaattta
     1621 acatcgttcc ataattgggt taacaaactg cgattagatg ggcgatcgga ttttaataca
     1681 actttttgcc ccaacaattt tcctccagca tagatgtaat cgcctttgct tttgtagatt
     1741 ggaacagatt ctccggttac aaaactgtag tcaattttag ctgtttcgct ttgcaaaata
     1801 ctatcacaag gaattatttc ttcgttgcga atgagaattt tatctcccac attaattttt
     1861 tcaatttcaa caatttcctc tccgttatcc gtaatttttg taaccgcaac cggaaaatac
     1921 gaagtgtaat cgcgctcaaa cgacaatgat ttataggttc tattttgaaa ccattttcca
     1981 atcaataaaa agaaaatgaa tcctgcaaaa gaatccatgt aacccggacc accttcggtg
     2041 attattgtaa acacactttg agaatacaaa gcaatgatac caatagcaat tggaacatct
     2101 aaatttaaac ttttactttt gatggatttg taggccgaaa taaaatagtc gcgagcagag
     2161 aatagcaaca cgggcacaga aaccaacaac gataggatgg aggtgaaaaa acggatttta
     2221 taatccatgg tgtcgatgcc taaatattcc ggaaaactcc acagcataat gctaccaaaa
     2281 gcaaaagcag caatccctaa tttatagaaa aatactttgt ctattttttt agattgctct
     2341 tttcggtttc caaagttggg tgcataacct attttattta aaaaatgtgc taattccgaa
     2401 agttgcaatt cattttttct atacgtaatt aatgcctcgc gttgtacaaa attcacctgg
     2461 catgaagtaa ccgctggcaa aattttgggt aaattttcta ataaataaat gcaacttgaa
     2521 cagtgaattt cgggtaaaaa gagggtgatt ttaacaaaag tatcgtcttc aaagtcaata
     2581 aaacgtgctt ttatctctgg aacttctaaa aaatcgtatt tgtgttgttg aattttttgt
     2641 ggacgaaccc ccgatttttc ttccaattga taaaaattcc ccaactgatt atcggtgagc
     2701 aactgatata ccgtcatgca tccattgcaa caaaatgact tttctttata agtataatgt
     2761 tgattaccaa taatttcatc accacaatgg tcacatgata gatttttttc catttttgta
     2821 aaaattcaag tgcaaagtaa cgatgaaagt tgattttaaa aaatgataaa aatcatattc
     2881 aactggaatc ttactttttt acacaaattt attggaattg cttgttagat taaaaaaata
     2941 gtatatcttt gcaatccgaa tttttgataa aaaacgcaat tataagattt aggatatgaa
     3001 aagaacgttt caaccttctg taagaaaaag aagaaataag catggattta gaagccgtat
     3061 ggctactaaa aatggacgta gagtattagc tgctcgtcgc cgtaaaggaa gaaaaaaatt
     3121 gactgtatca gatgaaccaa tgcacaagcg ttaatcgttt atctgtttaa atattttagc
     3181 cgattctgca aagtatcggc tttttttgtt aataacaata acactactgc atgcacgttt
     3241 gtgcaacatg ttttttcata cgattttgaa ttttaattaa acaatcgatt taaaatttaa
     3301 ttgaaactat aaatcaaatt ttaaaccctt cttatttaga atgatttaaa attaataaaa
     3361 attcaacgag aaaacaagaa ttaggacaaa agaatgcgat ttattttttt tgttgattgc
     3421 tccatcttaa tgcaataata aaaggcaatc caatgcagat aataaatgcc agtgcatagc
     3481 cagttggttt caaataaaaa tcgttgacat tttccaaatc gccttcgtta aaacctttgc
     3541 ctatgaaatg cgaaaggctt aatggcgctt caattgtaac tgcttgcaaa ccgttttgaa
     3601 attcaatgac gcctgggaat aattgaatag ggaagaacca taatggtata ttgacaatta
     3661 acaagcaaat gacaaagatg agggcgaatt ttaatttatt atttttcatt ttaaaacgaa
     3721 ctgaaggcga aaattaatca aaaagtaggg attcaaacaa ataaagcgct acatttatcg
     3781 ttttcattgc agaaattacc gcatgaattt atatacaaac aaacaaaagt ggaagattgt
     3841 actgttaatt gttgccctgc tattggttgg ggcttcgttg tttgtatcca atgaaattgt
     3901 ttccaaagta gctgctcaag aacgtcaacg ggccaaacaa tgggcatcca tgattaaaaa
     3961 aagggtagag ttgatacagt taaccgaccg tacttttacc caattgcgcg aaaaggaaaa
     4021 agaaaaaatg aagctgtggg tggatgcttc caaagagatt gctaatcctt ctgcgtattc
     4081 cattcctgaa tttccaatgc aaattgtgaa agacaacaac gatattccag ttattctttt
     4141 ggacaatgat gcaaatattt cgggctaccg caacttagat tttgacacat ccaacattcg
     4201 tcaagtttat cctgaattat cttccaaaaa attgattaaa aaatttgatg attctttgcg
     4261 cgtattggcg ggcgtatggg agaaaaaaaa tccagcgttt actgttgaag tgtaccaagg
     4321 actttttatg aaatatgtgt acgacgattc aaaggaaatt agtcgtttgg aaaaagaccg
     4381 cgattcgttg tttaaatcat tcaacaaaga aattattgaa gacgaggaat tggtacccgt
     4441 aatgttgatt aaccaagaaa cacgtcaggt tattggaacc aacatcaacg attctgtttt
     4501 tatgcacacc gatgtgaata gtttgattcg ggatatgtct aaaaccaacg actctattgt
     4561 gattgatttt agcaacaacg tgaaaaatgt attgtatttt gatagtagcc ccgttgttaa
     4621 acagctccaa tattttccct acatccaatt tgttatcatt ggtttgtttg tgtttattgc
     4681 ctatttgttg tttagcactt ttagaaaagc cgaacaaaat caggtatggg ctggaatggc
     4741 aaaagaaaca gctcaccaat taggaactcc gatttcatca ttaatggcgt gggtagagct
     4801 cttgcgttct caagaagtag atgcgatgat tgtggacgaa atgcataaag atattgaccg
     4861 tttgaacaaa gttaccgatc gtttctctaa aattggtagc gaagccaaaa tggagtccat
     4921 gaatttatcg gtaaccgttc aagaaattat tgattatctg caagttagaa catcaaaaca
     4981 agttcaattt acatccaaaa ttgaagaaaa tgttatggct ccccacagtt cttcgttgat
     5041 ttcatgggtg atagaaaaca ttgctaaaaa tgctattgat gctatggaag gaaaagggaa
     5101 gttgaacatt accttaaaat ccacatcaga aatggctgtt atttatattt cggataccgg
     5161 aaaaggaatg accccgcaac aaattcgctc tattttcaag ccgggctata ccaccaaaaa
     5221 acggggttgg ggattgggtt taaccttggt aaaacgtatt gttgaagaat accacaaagg
     5281 aaaagtggtg gtagaaaaat cgcaaatagg tgagggaacg acctttaa
//
LOCUS       DAOQVQ010000123         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17261_length_5270_cov_3.091275, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000123 DAOQVQ010000000
VERSION     DAOQVQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17261_length_5270_cov_3.091275"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="bshA"
                     /locus_tag="PKN22_05115"
     CDS             complement(<1..630)
                     /gene="bshA"
                     /locus_tag="PKN22_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNS42118.1"
                     /translation="MKIGMVLYPTFGGSGVVATELGKALAQKGHEIHFITYAQPVRLG
                     NFIDNTYYHEVSLSDYPLFDYQPYEIQLTSKIVDVVKHEKLDLLHVHYAIPHASAAYM
                     AQQILKAQGINIPFITTLHGTDITLVGKDPSFEPVITFCINESTAVTAVSESLKQSTY
                     EHFNTQRAIEVIPNFAPPIAKEMTFACQRLKYAMGEERILCHISNFRPVK"
     gene            complement(721..1584)
                     /gene="prmC"
                     /locus_tag="PKN22_05120"
     CDS             complement(721..1584)
                     /gene="prmC"
                     /locus_tag="PKN22_05120"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNS42119.1"
                     /translation="MFVSDNTVKSVKNYFSARLEGQFTAREIKIMFQLCAQQRLQLSS
                     ADLLLADNLRMSESDLLYFRSVVKRLQTNEPFQYILGEVEFYGLLLNTDKRALIPRPE
                     TEELVDWIVSKAPNATTIVDVCTGSGCIALALKSKCPAAKVVGVDVSIDALNLAKENA
                     KKTQLEVGFLQLDVLQQALPMDSKSIDVMVSNPPYVLENEKLQMANHVLDFEPALALF
                     VPNNDPLLFYKKIVHEATQKLKTGGFLFFEINEQYGNDVATLLNQSGFHSVEIKQDLQ
                     GKDRMVAGCLA"
     gene            complement(1584..2279)
                     /locus_tag="PKN22_05125"
     CDS             complement(1584..2279)
                     /locus_tag="PKN22_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42120.1"
                     /translation="MKTPFTLFIICVFCTIIAYSQEETIIVQSENEMVAIDEIKPKHT
                     FNVVLGLPNTTINKPFESIMKGVVDANVYYQYHLPFALVFGGGFRFIHYKINEFRVPE
                     KLDGVMHAFGPFFKIGYEKFYTKRFAFDASVKIGYEWNYVVTDLNKARRGGAYQFDAG
                     FVEPTIGFILSAANQSSFRLTIGYTIQGYSFKPAMLGTILQGGWEQKEFTKTAQFITF
                     GLGYTYYIGKKKD"
     gene            2461..4821
                     /locus_tag="PKN22_05130"
     CDS             2461..4821
                     /locus_tag="PKN22_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42121.1"
                     /translation="MRIFLLFLATVAVSTLGFSQDHNIRGFVFDKENGEPVTFEKVKL
                     FSSDSVMRGGAMTDINGLFSIPNVAIGEYYIAIETVEYEAVHQKVSVTKANGITELKF
                     NLEKIKNVKEIEGVHITAESQRNKTQIQVSKLSLDKATIERIPTIGAENDIVNALSIT
                     PGVITTGDQGGQLYVRGGTPIQNKILLDGMTIYSPFHSIGFFSVFETELIRNTDVYTG
                     GFDAKYGGRISSIMDITYRDGNRQKFGGKVSVSPFMAKAVLEGPMGKKKEDGSPRTGS
                     YILSAKHSLLSYTSKSLYRNINNMSTSSGAGMPYTFTDVYGKLSFNGDGGTKFSVFGF
                     HNRDSVNYAIADLSWQATGGGINFLLVPSSSKTIIKGHLTGSYYKTTFREAETQPRFS
                     GVGGFDLAFDFSYFLLKESELTYGLNIGGFNTNFTTYNEAKRKIEDANFSTEIGAYVS
                     FRYVASRWVVQPSFRLQAYPSVGTVVPEPRLGVKFNATEFMRVKLSGGRFSQNFTSAS
                     SDKDIVNLFNGMLSAPTNIQSTFVNQYGQEKTLKNGLQTAWHAVLGFEFDIVKNLSLN
                     VEGYYKFFDQLSNINQNKIYEDIPEFNNKSDVEKKDFLIESGESYGLDVLLKYNLNNS
                     LHVWTVYSFGYSKRWDGFEYYFPVFDRRHNLNLVVSYAFGKKKNTEIDLRWNYGSGLP
                     FTPTSGYYQPENFSGGITTDYTSTNSSNVGVLLGKFNSSRLPAYHRLDLTIKHKFIFK
                     NKMELELIASVVNLYNQKNIFYVNRVTNEVIYQFPILPSFGISWKF"
     gene            complement(4863..>5270)
                     /locus_tag="PKN22_05135"
     CDS             complement(4863..>5270)
                     /locus_tag="PKN22_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNS42122.1"
                     /translation="RIDGLIKEYRPHELAIEAPFFGKNVQSMLKLGRAQGVAIAACLS
                     NGMSYEEYSPRRIKQAVTGNGNASKEQVAAMLQKLFQIKDMPKYMDATDGLAAAVCHH
                     FSKGVGENTKTKEKGWGSFLKNNPERKVDFKTK"
BASE COUNT         1650 a          931 c          957 g         1732 t
ORIGIN      
        1 tttcacgggt ctgaaattgg aaatatgaca taaaattcgt tcttcgccca ttgcatattt
       61 taaccgttgg caagcaaaag tcatttcttt ggcaattggc ggtgcaaaat tggggataac
      121 ttcaattgca cgttgagtgt taaaatgttc gtaggtggat tgctttaaac tttcagaaac
      181 cgcagtaacc gctgttgatt cgttgatgca aaaagtaatc accggttcaa aagatggatc
      241 tttccccacc aaggtaatat ctgtaccgtg caaggtggta ataaacggaa tattgatgcc
      301 ttgtgctttt aaaatttgtt gagccatata ggctgccgaa gcatgtggaa tggcataatg
      361 cacgtgtaac aaatctagtt tttcgtgttt tactacatct acaattttac ttgttaattg
      421 aatttcatac ggttggtaat caaacaaagg ataatcgctc aacgaaactt catgataata
      481 cgtgttatcg ataaaatttc ccaaacgaac aggttgcgca taggttataa aatgaatttc
      541 atgccctttt tgtgccaatg cttttcccaa ctccgttgcc actactccac ttcctccaaa
      601 ggttggatat aaaaccattc ctattttcat atcgatatta tgtaaattga ggcaaaatta
      661 gcgaatattc gcttgcaaga attttaataa agtgttattt aatcttaatt tcaataataa
      721 tcaagctaaa caaccggcta ccattctgtc ttttccttgt aaatcttgtt ttatttcaac
      781 cgaatgaaac cccgattgat ttaaaagtgt tgcaacgtca tttccatatt gctcgttgat
      841 ttcaaagaaa agaaagcctc ccgtttttaa tttttgggta gcctcgtgca ctattttttt
      901 ataaaacaac aaggggtcgt tattgggaac aaacaaagcc aacgcaggtt caaaatccaa
      961 tacatgattg gccatttgca atttttcatt ttccaataca tagggcggat ttgaaaccat
     1021 cacatctatt gatttagagt ccattggcaa agcttgttga agcacatcaa gttgtagaaa
     1081 tcccacttca agctgtgttt tctttgcatt ttctttggct aaattcaatg catctatcga
     1141 aacatccaca cctacaactt ttgctgctgg acatttactt tttaatgcca aggcgatgca
     1201 accacttcct gtgcacacat caactattgt tgtggcattt ggcgcttttg aaacaatcca
     1261 atctaccaat tcttcggttt ctggtcgtgg aattaacgca cgtttatccg tattcaacag
     1321 caatccataa aactccactt ctcctaaaat gtattgaaaa ggttcgttgg tttgcaagcg
     1381 tttcacaacc gatcgaaaat agagtaaatc ggattcgctc atgcgcaaat tatcggcaag
     1441 caacaaatcg gctgatgaaa gttgcaagcg ttgttgggca cacaattgaa acataatttt
     1501 tatttcacgt gcagtgaatt gcccttctaa ccgtgctgaa aaatagtttt ttacagattt
     1561 tactgtatta tcagaaacaa acattaatcc ttctttttac ctatataata ggtgtatccg
     1621 aggccaaagg taataaattg tgctgtttta gtaaattctt tttgttccca accaccttgt
     1681 aaaatggttc ccaacatcgc cggtttaaaa gaataacctt gaatggtata gccaattgtg
     1741 aggcgaaacg aagattgatt agccgcactt aaaataaacc caattgttgg ctccacaaaa
     1801 ccagcatcaa attgataggc gccccctctt cttgctttat tgaggtcggt gacaacataa
     1861 ttccactcgt aaccaatttt tacagaggca tcaaaagcaa aacgtttggt gtaaaatttt
     1921 tcataaccta ttttaaaaaa aggtccaaaa gcatgcatca caccgtccaa tttttcgggc
     1981 actctaaatt cgttgatttt atagtgaata aaacgaaatc ctccgccaaa aacaagagca
     2041 aatggcaagt gatattgata gtaaacattg gcatctacta ctcctttcat aatactttca
     2101 aatggtttat tgatggttgt attcggcagt ccaagtacaa cattaaacgt atgtttaggt
     2161 ttaatttcat cgatggcaac catttcgttt tcagattgaa cgataattgt ttcctcttgc
     2221 gagtatgcaa ttatggtaca aaaaacacat attatgaata gagtaaacgg agttttcatc
     2281 acaaaaagat taatattttc attgaatacg taaatataat ggaatttatt tgattcaatt
     2341 caaaatatat cccgtttaat caaaaattga agcactttaa attaaaatct atttttgagg
     2401 tatatattct cgttggaaaa ttgtattttc gtcatctgtt tttaattttt tacaaactat
     2461 atgagaattt ttcttctttt tctagcaact gttgctgtat caacacttgg tttttcgcaa
     2521 gatcacaaca ttcgtggatt tgtttttgat aaagaaaatg gcgagcctgt tacttttgag
     2581 aaagtaaaat tattttcttc cgacagtgtg atgcgtggcg gtgcaatgac tgatatcaac
     2641 gggttgtttt ccattccaaa cgtagctatt ggtgaatatt acattgcgat tgaaaccgtt
     2701 gaatacgaag ctgtgcatca aaaagtgagt gttacaaaag caaatggtat tacggaatta
     2761 aaattcaact tagaaaaaat taaaaacgta aaagaaattg aaggtgtgca tattactgca
     2821 gaatcacaac gcaataaaac gcaaattcag gtttctaagt tatcgttaga caaagcaacc
     2881 attgaacgta ttcctaccat tggagcagaa aatgacattg taaatgcatt gagtattaca
     2941 ccaggggtaa ttacaacagg cgaccaagga ggacaattgt atgtgcgtgg tggtacacca
     3001 attcaaaata aaattttatt ggacgggatg actatttatt caccgtttca ctctattggt
     3061 ttcttttcgg tttttgaaac agaattaatt agaaacaccg acgtttatac aggtggtttt
     3121 gatgcaaaat atggcggacg aatttcatcc atcatggaca tcacctaccg cgatggaaac
     3181 cgtcaaaaat ttggaggaaa agtttctgta tcgccgttta tggcaaaagc cgtattggaa
     3241 ggtccaatgg gtaaaaagaa agaggatggt tctccacgta ctggttctta tattttatct
     3301 gcaaaacata gtttattgag ctatacatcg aaatcgttgt accgcaacat caataatatg
     3361 agcacatcat cgggtgctgg aatgccttat acatttaccg atgtgtatgg taaactttcg
     3421 tttaatggtg atggaggaac aaaatttagt gtatttggat ttcacaacag agatagcgtc
     3481 aactatgcta ttgcagattt gagctggcaa gcaactggtg gaggaattaa tttcttattg
     3541 gttccatcaa gttcaaaaac cattatcaaa ggacatttaa caggaagtta ttacaaaaca
     3601 acttttagag aagctgaaac acaaccacgc tttagtggtg ttggaggttt tgatttagcg
     3661 tttgactttt cctatttctt attaaaagaa agtgaattaa cctatggttt gaatattggt
     3721 ggatttaata ccaactttac tacctataac gaggcaaaac gcaaaattga agatgctaac
     3781 ttttctacag aaattggtgc ttatgttagt ttccgctatg ttgcaagcag atgggtggta
     3841 caaccaagtt tccgtttaca agcctatcca agtgttggaa cagttgttcc agaaccgcgt
     3901 ttgggagtta aattcaacgc aacagaattt atgcgtgtta aattgtctgg aggacgtttc
     3961 tctcaaaatt tcacatcggc ttcatccgat aaagacatcg tgaatttgtt taacggaatg
     4021 ctttctgctc cgacgaatat tcaatcaact tttgtgaatc aatacggtca agaaaaaact
     4081 ttgaaaaatg gtttgcaaac ggcttggcat gctgttttag gatttgaatt tgatattgta
     4141 aagaatttgt ctttgaatgt ggaaggatat tataaattct ttgaccaatt gagcaacatc
     4201 aaccaaaata aaatatacga agacattcca gagttcaaca acaaaagcga tgttgagaaa
     4261 aaagatttct tgattgaatc gggtgaatcg tatggtttgg atgttttgtt gaaatacaac
     4321 ttaaacaaca gtttacacgt ttggaccgtt tattcatttg gatattctaa acgttgggat
     4381 ggttttgaat attatttccc agtatttgac cgtcgtcaca acttgaattt ggttgtatcg
     4441 tatgcttttg gcaagaaaaa gaatacagaa atcgacttgc gttggaatta tggttctggt
     4501 ttgccgttta caccaacttc gggatattac caaccagaaa acttctctgg tggaattaca
     4561 accgattata catctaccaa ctcaagcaat gtgggtgtat tgttgggtaa attcaactca
     4621 tctcgtttac cggcatacca ccgtttggat ttgacaatta agcataaatt tattttcaaa
     4681 aacaaaatgg aattggaatt gattgcaagt gttgttaacc tttacaatca gaaaaatatt
     4741 ttctatgtaa accgtgttac aaacgaagta atttatcagt tcccaatttt accaagtttt
     4801 ggtatcagct ggaaattcta atatttttag tgtgtaaaca aatacgaaac ggaaggttgt
     4861 gcttattttg ttttaaagtc aactttccgt tctggattat tttttagaaa tgatccccac
     4921 cctttttctt ttgttttggt attttcacct acaccttttg aaaaatgatg acaaacagca
     4981 gctgccaaac catcggttgc atccatgtat ttaggcatgt cttttatttg aaataatttt
     5041 tgtagcatgg ctgcaacttg ttccttggat gcatttccat ttcctgtaac agcttgtttt
     5101 attctacgtg gcgaatattc ttcatagctc ataccattgc ttaaacatgc agcgatggca
     5161 actccttgtg cgcgacctaa cttcaacatt gattgcacat ttttaccaaa aaatggagct
     5221 tctatggcta attcgtgtgg acggtattct tttatcaagc catcgatgcg
//
LOCUS       DAOQVQ010000124         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17538_length_5212_cov_4.357766, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000124 DAOQVQ010000000
VERSION     DAOQVQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17538_length_5212_cov_4.357766"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKN22_05140"
     CDS             complement(<1..900)
                     /locus_tag="PKN22_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS42123.1"
                     /translation="MKSYNILTIGLLAVTFTACKGKEAAFERPTQPYKVIQLQQQEVE
                     LYSEYPAILQGVEDIEIRPMINGYIEKIYVIEGQEVKKGDVLFKISNPQFGQDVNMYA
                     ARIESAKAAVEAARVQVEKTKPLVEQGIISTYELTNVKLNYDMRKADLEQAKATYANA
                     QTNLGYTTIKSPVDGFVGKLPFKLGSFVYSNTAQPLTVVSNISKVYAYFSINEKQQLK
                     MFETTEGKNFQEKIAKIPPVTLILSNDSTYNHQGKIETFSGLINNQTGSFNVRAGFEN
                     PEKMLRSGSSTTIRIPTHLKDVII"
     gene            complement(897..1496)
                     /locus_tag="PKN22_05145"
     CDS             complement(897..1496)
                     /locus_tag="PKN22_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS42124.1"
                     /translation="MSNQLTDTELLIISKTKELVYNEGKIKPTSQEIADFCGVQRTLV
                     NYYFRSKENLTRIVQEEITSELHEGLNEIYGAQNVTFEQKVDALIDFMFNFKRKYSYF
                     EVYSINISTKLFDEEVYIKPEPTKMLCDFMQEINQEMEKGNILKSDPINFLINIFSLV
                     SYPLVMKGIYQDVFGISAEEFDQLLINRKNIIKNLIFNK"
     gene            1713..2711
                     /locus_tag="PKN22_05150"
     CDS             1713..2711
                     /locus_tag="PKN22_05150"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNS42125.1"
                     /translation="MKSDKKIAVIGGGSWATAIVKILCNNVQSVNWWMRNEASVRHIL
                     KYQRNPNYLQSVEFDLDKINVSTDLVEMIAPADIVIIATPSAFLVQTFENFPVELFKG
                     KIVFSAVKGIVPEYHAIPARFIHKTFGTPYNHIGIICGPCHAEEVAMERLSYLTIACQ
                     EDCIAEEVADLMSCRYIKTTTSDDLFGTELSAVLKNVYAIASGIFSGLGYGDNFQAVL
                     ISCAIQEIENFIDEVSPIHRDVKSSAYLGDLLVTSYSKFSRNRSFGFMIGKGYSVKTA
                     QMEMDMIAEGYYAVKSVVEINKRFEVDMPIVNAVYNILYEKISPVIEMRILTEKLS"
     gene            2764..4035
                     /locus_tag="PKN22_05155"
     CDS             2764..4035
                     /locus_tag="PKN22_05155"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNS42126.1"
                     /translation="MKVFKFGGASVKDAAAVKNVVEILRLFQSDKIVVVVSAMGKTTN
                     ALEVVVDAIWNKNASVFNQKIEELKNFHADIVNQLFGNEQQEVAALLTSVFDQLEAQF
                     DKELSDNYHCEYDQIVSLGEVISTIIVEAYFRHANITSKWMDARRLVRTNAAFRDAEV
                     DWKRTEELIQKNVIPAFENNQVVVVQGFVGHTSELLTTTLGREGSDFTAGIFAYCLNA
                     ESVTIWKDVPGMLNADPKFFKDTVKLDKISFNEAIELSYYGASVIHPKTLKPLQNKNI
                     PLFVKSFIEPTKQGTEIQQSAEFDTLVPSFIFKLNQTLISFRTRDFSFIVESHLSDIF
                     NQLSALGVKINLMQNSALSFSILVDAQKVHMPSVLSVFQQNYEVKYNEGLELVTIRHY
                     DAPTIQRVTEGKERLLEQMDRLTARIVLKAN"
     gene            complement(4032..4697)
                     /gene="mnmD"
                     /locus_tag="PKN22_05160"
     CDS             complement(4032..4697)
                     /gene="mnmD"
                     /locus_tag="PKN22_05160"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNS42127.1"
                     /translation="MNYRVINTNDGSKTIYVEELDETYHSSHGALQEAVHIFINNGIK
                     SINQQQIALFEMGFGTGLNALLTAIYAQENNINVTYSGIEAYPVAFELAQEMDYCALI
                     GKEHEHLFSQLHQCKWDVLETITPNFSLQKIHAKIENYTLPSNQFDCIYFDAFGPRAQ
                     EDMWTIQILQKMYNALKPNGFLITYCAKGQVKRNLKSLGFEIEPLPGPPGKREITRAW
                     KRN"
     gene            complement(4672..5064)
                     /locus_tag="PKN22_05165"
     CDS             complement(4672..5064)
                     /locus_tag="PKN22_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42128.1"
                     /translation="MDYQVELRFKKLTALLNKDFDGDMDISGILFLIGVNELGWGFKN
                     YSKDEKMDLVHIATCTILAPYGYYELEGKDQDNWPHFKLIKKLPHLEYNQQQHLMKEA
                     IIDYFISNGYYTKEQITPTFSELSRHKH"
BASE COUNT         1595 a          848 c          970 g         1799 t
ORIGIN      
        1 gatgattaca tcttttaaat gcgtagggat acgtatggta gtgctagaac ctgagcgcaa
       61 cattttttca ggattttcaa aacctgctct cacgttaaac gaaccagttt ggttgttgat
      121 taacccacta aatgtttcaa ttttaccttg gtgattataa gttgaatcgt tggataaaat
      181 gagggttaca ggtggtattt ttgcaatttt ctcttgaaaa ttcttacctt ctgttgtttc
      241 aaacattttc aattgttgtt tttcgttgat agagaaatag gcatacactt tagaaatatt
      301 ggaaacaacg gttaatggtt gagccgtgtt gctataaaca aaactaccca atttaaaagg
      361 aagtttacct acaaaaccat ccacggggct tttaattgtt gtatatccaa gattggtttg
      421 cgcatttgca taagttgctt ttgcttgttc caaatccgct ttacgcatgt cgtaattcaa
      481 cttcacattg gttaactcgt aagtactaat aattccttgt tcaaccaatg gttttgtttt
      541 ttctacttgt acacgcgccg cctctactgc cgcttttgca ctttctatgc gcgctgcata
      601 catgttgaca tcttgtccaa attgtggatt tgaaatttta aacaacacat ctcctttttt
      661 cacttcttga ccttcaatta catatatttt ttcaatgtaa ccgttaatca ttggtcgaat
      721 ttcaatgtcc tcaactcctt gaagaattgc tggatattct gaatacagtt caacttcttg
      781 ttgttgtaat tgtataacct tgtagggttg agttgggcgt tcaaatgctg cttctttacc
      841 tttacaagct gtaaaagtta ctgctaataa gccaattgta agtatgttgt atgatttcat
      901 ttgttgaaga ttaaattttt aattatattt tttctattga ttaaaagctg atcaaattct
      961 tcggcagaaa tgccaaaaac gtcttggtat attcctttca ttacaagcgg gtaagatacc
     1021 aatgagaaaa tgttgattaa gaaattgata ggatccgact ttaaaatgtt tcctttttcc
     1081 atttcttgat taatttcttg catgaaatca cacaacattt ttgttggttc gggtttgata
     1141 taaacctctt catcaaaaag tttagtagaa atgttaatgc tgtacacttc aaaataagaa
     1201 tacttacgtt tgaaattgaa cataaagtct ataagtgcat ctactttttg ttcaaaagtt
     1261 acattttgtg caccataaat ttcattcaac ccttcgtgta attcggaggt gatttcttcc
     1321 tgaactatgc gtgttaaatt ttcttttgat cggaaataat aattaaccaa cgtgcgctga
     1381 acaccacaaa aatcggctat ttcttgcgat gttggtttta tcttcccctc attatacaca
     1441 agctctttgg tcttggaaat aatgagtaac tcggtgtctg ttaattggtt tgacataaat
     1501 gtttgacaaa tttgtcaaca aaagtacggc aactttttta attgaaaaaa tttattttca
     1561 aaacgaatgt taaaaattga ttttggatga tatcgaaata tgtcgatatt gcaatatgac
     1621 gatatcgcaa tatgacgata tggtaatttt aagaacccat tcacaaattc agtggttgta
     1681 atcaaaatat tctatacatt tgcagttcga ctatgaaatc ggataagaaa atagcagtta
     1741 ttggtggagg aagttgggca acagccattg ttaaaatttt gtgcaacaat gtacaatctg
     1801 tcaattggtg gatgcgaaac gaggcttcag tgcgacatat attaaaatac caacgcaatc
     1861 ccaactactt acaatcggtt gagtttgatt tggataagat caatgtcagc acagatttgg
     1921 tagaaatgat tgcaccggcg gatattgtaa tcattgcaac tccttctgct tttttagtgc
     1981 aaacgtttga aaatttccct gttgaattgt ttaaaggaaa aattgttttt tctgccgtaa
     2041 aaggtatagt gcctgagtat catgccattc ctgcacgttt tattcataaa acctttggta
     2101 caccgtataa tcacattgga attatttgtg gaccatgtca cgccgaggaa gtggctatgg
     2161 aacgtttgtc gtatctgacc attgcttgtc aagaagattg tatagccgaa gaggttgccg
     2221 atttgatgtc gtgcagatat ataaaaacaa ctacatccga tgatttgttt ggaactgaat
     2281 tatcggcagt attgaaaaat gtatatgcca ttgcctctgg aattttctca ggtttgggtt
     2341 atggtgataa ttttcaagcg gtattaatta gctgtgccat tcaagaaatt gagaatttta
     2401 ttgatgaggt gagtccaatt caccgcgatg tgaaatcgag tgcctatttg ggcgatttat
     2461 tggtaacttc ttattctaaa ttctcgcgca atcgttcgtt tggatttatg attggcaaag
     2521 gttattcggt aaaaacggct caaatggaaa tggacatgat tgccgaagga tactatgctg
     2581 taaaaagtgt ggtagaaatc aataaacgtt ttgaggtgga tatgccaatt gtaaatgccg
     2641 tttacaatat tttatatgaa aaaatatcgc ctgtaattga aatgcgtatt ttaaccgaga
     2701 aattgagtta aatcgttttg tatttcaaaa atctgtatct tcgtttttat aattctaact
     2761 tcaatgaaag tattcaaatt tggtggtgct tctgttaaag atgctgctgc ggttaaaaat
     2821 gtggttgaaa ttttacgctt atttcaatcg gataaaattg ttgttgtagt ttcagcaatg
     2881 ggcaaaacca ccaatgcttt agaagttgtt gtagatgcta tttggaataa aaatgcatcg
     2941 gtttttaatc aaaaaattga agaactaaag aactttcatg ccgatattgt caatcaatta
     3001 tttggcaatg aacaacaaga agtggcagct ttgctaacta gcgtttttga ccaattagaa
     3061 gcgcaatttg ataaagaatt atcggataat taccattgtg aatacgatca aattgtgtca
     3121 ttaggcgagg tcatttcaac tattattgta gaggcttatt tccgacatgc aaacattaca
     3181 tctaaatgga tggatgcacg acgattggtg cgaacaaatg ctgcatttcg cgatgccgaa
     3241 gtggattgga aacgcaccga agaattgatc caaaaaaatg ttattccagc gtttgaaaac
     3301 aaccaagtag ttgtggttca gggatttgtt gggcatacct ctgaattgtt gacaactaca
     3361 ttaggacgtg aaggttctga ttttacagcc ggaatttttg cctattgttt aaatgccgag
     3421 agtgtaacta tttggaaaga tgttccagga atgttgaatg ccgaccctaa attttttaaa
     3481 gatacggtga aattagacaa aatttcgttc aacgaggcca ttgaattgtc gtattacgga
     3541 gcaagtgtca tacacccaaa aacattaaaa cccctgcaaa acaaaaacat tccgttgttt
     3601 gtaaaatcgt tcattgaacc aacaaaacag gggacagaaa tacaacaatc ggctgagttt
     3661 gataccttgg tgccatcgtt tatttttaaa ttaaaccaaa cgttgatttc ttttcgcacc
     3721 cgagattttt catttattgt ggaatctcat ttaagcgata ttttcaatca actttcggct
     3781 ttgggagtaa aaatcaattt gatgcaaaat tcagcactta gtttttccat attggtagat
     3841 gcgcaaaaag tacacatgcc ttcggtttta tcggtttttc aacaaaatta cgaagtaaaa
     3901 tacaatgaag ggttagaatt ggtcaccata cgccattacg atgcaccaac aattcaacgt
     3961 gttactgaag ggaaggagcg attgttggaa caaatggata gattaactgc tcgcattgtt
     4021 ttaaaagcta attaatttct tttccacgca cgtgtaattt cacgttttcc tggaggtccg
     4081 ggcaacggtt ctatttcaaa accaagtgat tttaagttac gttttacttg tcccttggcg
     4141 caataggtaa ttaaaaatcc gttgggttta agtgcgttat acattttttg tagaatttgg
     4201 atggtccaca tatcttcttg ggcacgaggt ccaaaagcgt caaaataaat gcaatcaaat
     4261 tggttggaag gcaaggtata gttttcaatt tttgcatgaa ttttttgcaa agaaaaattg
     4321 ggggtgatgg tttccaaaac atcccattta cattggtgga gttgagaaaa caagtgttcg
     4381 tgttcttttc caattaacgc acaataatcc atttcttgag ccaattcaaa ggcaacagga
     4441 taggcttcta ttccggagta ggtaacgttt atattgtttt cttgagcata aatggcagtc
     4501 aacaaggcgt ttaaacctgt accaaacccc atttcaaaca atgcaatttg ctgttgattg
     4561 atggacttta taccattatt tataaaaata tgcaccgcct cctgaagcgc cccatgtgaa
     4621 gaatgatagg tttcgtcgag ctcttcaaca taaatggttt ttgaaccatc gttagtgttt
     4681 atgacgcgat aattcactga aagttggagt tatttgttct ttggtataat aaccattaga
     4741 gataaaataa tcaataatgg cttctttcat taaatgttgt tgttggttgt attcaagatg
     4801 tggtaatttt tttatgagtt taaaatgagg ccaattatct tggtctttcc cttccaattc
     4861 ataatatcca taaggtgcca aaatggtaca agttgcaatg tgcaccaaat ccattttttc
     4921 gtcttttgaa taatttttaa atccccagcc caattcgttt acaccaatta gaaacaagat
     4981 gcccgagata tccatatcgc cgtcaaaatc tttgttcaac agcgccgtca atttcttaaa
     5041 ccttaattct acttgataat ccatttttta agtatttatg caaaattaac aatagaaatt
     5101 gagcttccaa ttcttatttt gtaattttga agaaaataaa aagtttgtta tgaaaagata
     5161 ttttagaatt ttacccttct ttttgggatt aagcatgttg gttgcatgtg ga
//
LOCUS       DAOQVQ010000125         5210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17546_length_5210_cov_4.117168, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000125 DAOQVQ010000000
VERSION     DAOQVQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17546_length_5210_cov_4.117168"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKN22_05170"
     CDS             complement(<1..668)
                     /locus_tag="PKN22_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNS42129.1"
                     /translation="MKYTKPIFQIALTQLCGFGTIKSKQLLAAVENEEAIFALPLKKL
                     AQISGFNIEFLKKMERDKALEKANAIYASIQRFGIQSIFYTDAEYPRRLKQCADAPLV
                     LYKRGNIDLNNTRFVAVVGTREASDYGKKICTELIQQLEGSNIVVVSGMAYGIDITIH
                     QLCLQHHVQTIGVMAHGLERVYPHLHRNVAKKMMDNGCLLSEYPPGTVPDKENFPMRN
                     RIVAG"
     gene            742..3087
                     /locus_tag="PKN22_05175"
     CDS             742..3087
                     /locus_tag="PKN22_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42130.1"
                     /translation="MLPTLLFSQITGSVKDKSSNEAIVGAKIYSSNSKHAITENDGSF
                     IVSTQPNDFPVTLITSFAGYQNDTTIVSQPGAVQILLNVPAKMIETVVVTAGRRGQNI
                     EEVPVSIEVLRPQLIDNKGFKDLEDAIEQVPGVQTMDGQVSIRGGAGYAYGAGSRVMV
                     LWDGMPLVSGDAGDIKFNSIPMESASQIEVIKGASSVLYGSGALNGIVSLTERTPSEQ
                     GELRLKYQVGVYDNPKRASLKWWKTNPMFDLLDFYYGKKFNQVGFTISANKVTAKGYR
                     QGEDENRGRISGTFFYLPKQVRGLKVGLGFNAQLQRNNNFMLWENDSLGYQPMGGAIA
                     GDSASTLGFFRGIRANVDPYIKYIDKYNNSHSLKTRMYYVQNKNIYNAGQTSTSVVSY
                     ADYQFQRKFGFGLTLTTGLTGIHTDIKSNLYGKHNSMNAALYLQLEQKLWNKLDITAG
                     VRAEYYEQDGRQGDSYFYLKKDSTSKMPVYPIFRAGLHYEPVKGTHLRASYGQGIRYP
                     SVAERYTVTNVGALNIFPNPQLVAEKGWAAELGIKQIIPIGKNWKGIVDVAGFINQYQ
                     NMMEFEFGIFNPDSIPLSIDKNNPGYLGKWIGFRASNNESARITGLEASFSSIGTIGQ
                     VEFASMIGYTYMNPVSLNTDSMYLASLSSFKIDENGNPTYNKMLKYRFNHMFKGDVEV
                     TWKGVSVGFSSRYNSRVENIDRIFEESILGQYILPGLKKYREEYGKKGALVFDARVAY
                     KFLKHYKVSFIVNNILNTEYSTRPGDIQPPRTFILQVQATF"
     gene            3089..3820
                     /gene="kdsB"
                     /locus_tag="PKN22_05180"
     CDS             3089..3820
                     /gene="kdsB"
                     /locus_tag="PKN22_05180"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNS42131.1"
                     /translation="MKVVGIIPARYASSRFPGKPLIDLKGKSMIQRVYENASKATLLN
                     ELIVATDDQRIFDHVQAFGGKVVMTQAHHTSGTDRCGEVVQNIDADVVINIQGDEPLI
                     NPQQLDEIIAAFHDDKVQIATLGYPKISVEDINNPNRIKIVTDHNNDALYFSRSAVPN
                     DYHFKGDSKTFYPFLKHIGMYAFRRKTLLEVIALQPSKLEQVESLEQLRWLYYGYKIR
                     VVHTTIETPNIDVPEDVEKVLKLVE"
     gene            3858..4724
                     /locus_tag="PKN22_05185"
     CDS             3858..4724
                     /locus_tag="PKN22_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNS42132.1"
                     /translation="MKFGKTIKIFLIDGDPNGRMSCELSNWSGKAYKIPRIKVKDCSD
                     REDLIISTGVYLLFGKDNDGKDQVYIGEAETILKRLNQQLTSKDFWNETIVFISKDDN
                     LNKAHVKYLENRLHEIAKSANRYKVDNSIVPTQSSISESDRAEMEEFIEYIKLLVNTL
                     GHKVFEEKREFKPKQKQATFFIKATRGADGQGEPTSDGFVVFKGSKAAATIVNSMTSN
                     FITYRQKLIDEGVLVDKGEFFEFTDDYIFSSPSTAAVMVMGRNANGLTEWKTKDGKTL
                     KDFETNEKTTKP"
BASE COUNT         1710 a          937 c         1056 g         1507 t
ORIGIN      
        1 ccggccacaa ttcggttgcg catcggaaaa ttttctttgt cgggcacagt gccaggtgga
       61 tattctgaaa gcaaacagcc gttgtccatc atttttttag ccacattacg atgcaaatgc
      121 ggatatacac gctccaaacc gtgcgccatc acacctatag tttgaacgtg atgctgcaaa
      181 cacaattgat gaatggtgat gtcaattcca taggccatac cgctcaccac cacaatgttt
      241 gagccttcca gttgttgaat taactctgtg cagattttct taccataatc gcttgcttca
      301 cgtgtgccaa ccactgcaac aaaacgggtg ttgtttaaat ctatgttgcc ccgtttatac
      361 aaaaccaacg gcgcatcagc acattgtttc aaccggcgcg gatattctgc atcggtataa
      421 aaaatacttt gtatgccaaa gcgttgaatg gaagcataaa tagcatttgc tttttccaat
      481 gctttatcac gttccatttt tttaagaaac tcaatattaa atccagaaat ttgcgccagt
      541 tttttgaggg gaagtgcaaa aatagcttcc tcgttttcaa ctgctgccaa aagttgcttg
      601 gacttaatgg ttccgaaacc gcacaattgt gttagtgcaa tttgaaagat aggtttagtg
      661 tatttcaaaa ttttgttaat ttagtcactt gaaattacta actttttttg taaaatgaaa
      721 cacatctcca catttttttt catgcttcct actttattgt ttagccaaat tacaggttcg
      781 gttaaagaca aatcaagcaa tgaagccatc gttggagcaa aaatatattc gtccaattca
      841 aagcatgcaa taacagaaaa tgatggatcg ttcatcgttt ccacacaacc caatgatttt
      901 cctgttacat tgataacgtc atttgctggt tatcaaaacg atacaacaat tgtttcgcag
      961 ccaggagccg ttcaaatttt attgaatgtg cctgcaaaaa tgattgaaac agtggtggta
     1021 actgctggaa gacgcggtca aaacattgaa gaagtacccg tttctattga ggttttacga
     1081 ccacagttaa ttgataacaa aggatttaaa gatttagagg atgccattga gcaagtgcca
     1141 ggcgtgcaaa caatggacgg acaagtaagt atacgtggag gagcaggtta tgcttatggt
     1201 gcaggtagca gggtaatggt actttgggat ggaatgcctt tggtttctgg agatgcaggc
     1261 gatattaaat ttaattctat cccgatggaa agtgcttcgc aaattgaagt tatcaaaggt
     1321 gcttcttcgg ttttatatgg tagtggtgct ttgaacggaa ttgtttcact caccgaacgc
     1381 acaccgtcag agcaaggaga attgcgtctc aaatatcaag taggtgttta cgataatcca
     1441 aaacgggctt cattaaaatg gtggaaaacc aatccaatgt ttgatttgtt ggatttctat
     1501 tatgggaaaa aattcaatca agtaggtttt actatctctg ccaataaagt tacagcaaaa
     1561 ggatatcgcc aaggagagga cgaaaacaga gggcgtattt ctggaacgtt tttctatctt
     1621 cctaaacaag tgcgcggttt gaaagttgga ttaggattca acgctcaatt gcaacgcaac
     1681 aacaatttta tgctgtggga aaacgattct ttgggttatc aaccaatggg tggggcaatt
     1741 gcaggtgatt ctgcttcaac cttgggattt tttagaggaa ttcgtgccaa tgttgacccc
     1801 tacatcaaat acatagacaa atacaacaac tcgcacagct tgaaaacacg tatgtattat
     1861 gtgcaaaaca aaaacatata caacgccgga caaacttcaa catctgttgt gtcgtatgcc
     1921 gactaccaat ttcaacgcaa atttgggttt ggattaacac taacaacggg gttaacaggc
     1981 attcacaccg atattaaatc caatttatac ggtaaacaca attcgatgaa tgccgcgctt
     2041 tatttgcaat tagaacaaaa actttggaat aagcttgaca tcacagcggg agttcgtgca
     2101 gaatattacg agcaagatgg acgacaaggc gattcttatt tttatttgaa aaaagattct
     2161 acctcaaaaa tgccggttta cccgattttt agagcaggtt tgcattacga acctgtaaaa
     2221 ggaacccact tgcgagcttc ttatggtcag ggaattcgct atccttctgt tgcagaacgc
     2281 tatacagtaa ccaatgttgg ggcgttgaat atttttccaa atccacaatt ggtagcagaa
     2341 aaaggttggg cagccgaatt gggcattaag caaatcatcc ctattggaaa aaattggaaa
     2401 ggaattgttg atgtggctgg ttttatcaat caataccaaa acatgatgga gtttgagttt
     2461 ggtattttta atcccgatag tattccactt tccattgata aaaacaatcc gggttatttg
     2521 ggtaaatgga taggatttag agcatccaac aatgaatcgg ctcgtattac aggacttgaa
     2581 gcctcttttt catcaattgg aaccattggt caggttgaat ttgcttctat gattggctat
     2641 acctatatga atcctgtgtc attaaacacc gattctatgt atttggcaag tttatcatcg
     2701 tttaaaatag atgaaaacgg caatccaacc tacaataaaa tgttgaaata cagattcaat
     2761 cacatgttta agggcgatgt ggaagtaact tggaaaggtg tgagtgttgg attttcttcg
     2821 cgctacaata gccgagttga aaatattgac cgtatctttg aggaatccat cttggggcag
     2881 tacattttac cgggattaaa aaaataccgc gaagaatacg gtaaaaaagg agcattggtg
     2941 tttgatgcac gtgtggcata caaatttttg aagcattaca aagtttcatt tattgtaaac
     3001 aatattttga ataccgaata ttctacacga cctggagata tacaaccacc gagaacgttt
     3061 attttacaag tacaagcaac attttaacat gaaagtagta ggcattattc cagcgcgtta
     3121 tgcatcgtct cgttttccag gcaaaccatt aattgattta aaaggcaaat caatgattca
     3181 acgggtgtat gaaaatgcat ccaaagctac attattgaac gagttgattg tggcaacaga
     3241 tgaccaacgc atttttgacc acgtgcaagc atttggagga aaagtggtaa tgacacaagc
     3301 acatcatacc agtgggaccg atcgttgtgg tgaggttgta caaaatattg atgccgatgt
     3361 tgttattaac atacaaggcg atgaaccgtt gattaatcca caacaattgg atgaaattat
     3421 tgctgctttt cacgatgaca aggtgcagat agcaacgctt ggttatccta aaattagtgt
     3481 ggaggatatt aacaacccta atcgcataaa aattgtaacc gaccacaaca atgatgcgct
     3541 ttatttttca cgcagtgctg ttcccaacga ttaccatttt aaaggcgatt ccaaaacatt
     3601 ttatcctttt ctaaaacaca ttgggatgta tgcttttcgt cgcaaaacat tgttggaagt
     3661 aattgcattg caaccttcta aattggagca agtagaatcg ttggagcaat tgcgctggtt
     3721 gtattatggc tacaaaattc gtgtggtaca cacaacaata gaaactccca acatcgacgt
     3781 tccagaagac gttgaaaaag ttttgaagtt ggtggagtag gatggtatgt ctcattgtaa
     3841 aaaacagata actaaacatg aaattcggca aaacaataaa aatattccta attgacggtg
     3901 acccaaacgg acgaatgagt tgtgaacttt ccaattggtc tggaaaagct tataaaattc
     3961 caagaattaa agttaaagat tgctctgacc gagaagactt aataataagt acaggtgttt
     4021 atttgctatt cggcaaagac aatgacggaa aggatcaagt ttatattgga gaagccgaga
     4081 ccattctaaa aagactaaat caacaattga catccaaaga cttttggaac gaaactattg
     4141 tattcataag caaggacgac aatctgaata aagcacatgt aaagtacttg gaaaacagac
     4201 ttcatgaaat agcaaaatca gcaaacagat ataaagttga caattctatc gtgccgacac
     4261 agtcttcaat ttctgaatca gacagagcag aaatggaaga attcattgag tatataaaac
     4321 ttctagtaaa cacattagga cacaaggtat ttgaagagaa aagagaattt aaacctaaac
     4381 agaaacaggc aacattcttt atcaaagcaa cacgtggagc agatggacaa ggagaaccga
     4441 catcggacgg atttgtagtt ttcaaaggct ccaaagctgc tgcgactatt gtaaattcaa
     4501 tgacttcaaa ctttatcact tacaggcaaa aactcataga cgaaggcgta cttgttgaca
     4561 agggagaatt ttttgaattc acagacgatt atatttttag cagtccatcg acagcagcgg
     4621 taatggtaat gggacgaaat gctaacgggt tgactgaatg gaaaactaaa gacggcaaga
     4681 cattaaagga ctttgaaacg aatgagaaaa caacaaagcc atagcacacg ttttgcgtca
     4741 taagtgatga cgtgcttaca ttcaagttct gttttccatt tgtgcttttg tgatgggttg
     4801 acagctttgt acttcaaaaa ctctcacgaa tataaagcgg gggaacggta tttgcacaaa
     4861 aatagtttga tttattgttt agtggctact tctattacac ttatcacctc ccttaatccc
     4921 tcctcaagga gggaaacacg aacgttctta aaacaacgaa caatctataa ccaatcaaaa
     4981 ggattaaaaa cctccctcct tgaggaggga aagagggagg tgaccaagaa tatttttatt
     5041 gcttttttaa ttttaggcag tgaatagcat gtaaaatttg tgtagttaca tcatccaatt
     5101 ggtttaaaac agctccttca ctaaaccgaa gaaaagtata gcccagttgt tgtaattttt
     5161 gttgacgata gatgtcgtat ttggcattat gataatgtga atttccatca
//
LOCUS       DAOQVQ010000126         5200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17599_length_5200_cov_4.262974, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000126 DAOQVQ010000000
VERSION     DAOQVQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17599_length_5200_cov_4.262974"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PKN22_05190"
     CDS             <1..1178
                     /locus_tag="PKN22_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS42133.1"
                     /translation="SGLSTIVQETFTGIGVIKAYERENEVNDRFVDASKTYKKKQMNL
                     VLINALFQPTILLLIGISTILSIYVGGLFSFTGDISYGGITAFVFFVGSLTWPFASLG
                     WLTSVIQRAAASQERINEFLKTKPEITNPTTQPFLYNGKIEFKNVTFTYKGMAEPAIK
                     NLSFTIHPHETVAFVGRTASGKSTILKLLLRQIEPQSGEILIDNQPLNSINLEQFREQ
                     VGIVPQEVFLFSDTIANNIRFGANHEVSNEEIIKATELAHIRHNIEAFENGYETLLGE
                     RGMNLSGGQKQRISIARAFIRHPKLLVLDDCLSAVDTETEEIILRHLANDTHTLNTII
                     VSHRISSLRNAQKIIVLKNGEKAEEGSHEELMKIDGIYCDMYNKQLVESDNSADTIA"
     gene            1350..1646
                     /locus_tag="PKN22_05195"
     CDS             1350..1646
                     /locus_tag="PKN22_05195"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNS42134.1"
                     /translation="MMENESGGEVFSKAIRAGKRTYFFDVKTTKGNDLYLTVTESKKI
                     QYDGKESYQKHKIFLYKEDFEKFKDGLLEALEKIDELKDEYPSTERSDLNFEDL"
     gene            complement(1648..2082)
                     /locus_tag="PKN22_05200"
     CDS             complement(1648..2082)
                     /locus_tag="PKN22_05200"
                     /inference="COORDINATES: protein motif:HMM:NF018037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HNS42135.1"
                     /translation="MQKIFTSIVFIGIICNGLFAQLYFKNNKSQTYKVAYVFHDKGSQ
                     YSGWISKGWVVVQPGEQVKAFYTNPRDKDMYCIAISENDTIGGDFCFLVHPTMSDFTL
                     KYPELEATQQQNPALEWMNFKEIKRNYVVKFKQKLVITIGDE"
     gene            2168..2791
                     /locus_tag="PKN22_05205"
     CDS             2168..2791
                     /locus_tag="PKN22_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNS42136.1"
                     /translation="MHFKQVDTSVELPIMEHFYSVQGEGFYSGRPAYFIRLAGCDVGC
                     VWCDVKESWDVEEHPVVQLDDLMQQIKATAANFVVITGGEPALYDLRGLIDRLKQANI
                     TIAIETSGTSELVGNVDWYCFSPKKFKKPVDEAYQKASELKIIIFNKSDYKWAEEHAA
                     KVNANCQLYLQPEWSKQEQFLPQIIEYVKNNPKWRISLQTHKFMQIP"
     gene            2794..4803
                     /locus_tag="PKN22_05210"
     CDS             2794..4803
                     /locus_tag="PKN22_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS42137.1"
                     /translation="MRLLTLIFSIVLVASCSISQNYSSTNKKAIKLFEEAMKAPSLSI
                     DPVRRTPNFRAGIELAEKAIAKDPRFWEAHLLAAELYENYYEYAAAIKHYEAALAINP
                     NHAPSGSTYFYLGNSQFAVGDYQGALKNLEIYNKNRSADPRMLQAANQIMDNCEFALD
                     AIKNPAKYNPINVGPGINTADPEYFPTITVDGKTILFTRRIKDPNSPVGMHEDFFVSN
                     SEDGKKWQTAVPMPKNINTTNNEGAPTISADGRSLIFVACSDESGRNYGPNREGWGSC
                     DLFYTKKLGTRWLDPINLPGKVNTSLWESQPSLSADGKTLYYVRRVSKPGESPNSDIF
                     VSYLQEDGTWSAGKPLPTTINTKFLEESVYIHPDGKTLYFASRGHKNLGGSDLFVSKM
                     DKDGKWGPAVNLGYPINTQYDENSLLVSPTGEIAFFASDREGGYGDLDIYYFELPEEL
                     RPTKTVYFDGIVFDQSNKMPLPGKFELIDIESGAVVMNSEADKINGQFMVSLPVNKEY
                     VLNVSYPGYLFFSQNFNMTLPDNQEAFHMDVPMVSITADIPVSLKNVFFDLAKATLRP
                     ESHIELNKLVDFMVKNPSLKIEIAGHTDTRGDAKANLILSQDRANSVVQYLIEKGINA
                     SRLVAKGYGESKPLIADAEIEKMATEQEKEIAHQENRRTEYRIIR"
     gene            4800..>5200
                     /locus_tag="PKN22_05215"
     CDS             4800..>5200
                     /locus_tag="PKN22_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNS42138.1"
                     /translation="MKHYLKLTRPLNLLIICFTMVGVRHYLMVTNGETYFWEWSFALL
                     VLSMVLIAGAGNVINDYFDVETDRINKPEKLIVGKYITSKQAMRYYIALNSTAVLIAL
                     FLCWHLETWVFAVFHPLLVLLLYLYSYYFKKT"
BASE COUNT         1696 a          956 c         1000 g         1548 t
ORIGIN      
        1 aatctggact ctccaccatt gtgcaagaaa cctttactgg aataggtgtc atcaaagcgt
       61 atgaacgcga aaatgaagtc aacgaccgtt ttgtagatgc ttcaaaaacc tataaaaaga
      121 aacaaatgaa cttggtgtta atcaacgctt tgtttcagcc aaccatttta ttgctcattg
      181 gtatttctac cattctttcc atctatgttg gtggtttgtt tagctttact ggcgacatca
      241 gctatggtgg aataacggcg tttgtatttt ttgttggttc actcacgtgg ccttttgcaa
      301 gtttgggttg gctcacatcg gttattcaac gtgctgccgc atcgcaagaa cgcatcaatg
      361 aatttttaaa aaccaaacca gaaataacca atccaactac tcagccgttt ctgtacaacg
      421 gaaaaattga gtttaaaaat gttactttca cctacaaagg catggcagaa cctgctatta
      481 aaaatctaag ttttaccatt catccgcatg aaacagttgc gtttgtagga agaacagcta
      541 gtggaaaaag tactatttta aaattattgt tgcgccaaat agaacctcaa agtggagaga
      601 ttttaataga caatcagccg ttgaacagca tcaatttaga gcaatttcgt gagcaagttg
      661 gaattgttcc gcaagaagtg tttctattca gcgataccat tgccaacaac attcgttttg
      721 gagccaatca cgaggtgagc aacgaagaaa ttatcaaagc aaccgaattg gcacacattc
      781 gccacaacat tgaagccttt gaaaatggtt acgaaacatt gttgggtgaa cgcggaatga
      841 atttaagtgg tggtcaaaaa caacgcatta gtattgcccg tgcatttatt cgtcatccaa
      901 aacttttagt gctcgacgat tgtttatctg ccgttgatac agaaaccgaa gaaatcattt
      961 tgcgccatct ggcaaatgat acgcacacgc tcaacaccat tattgtttca catagaattt
     1021 cctccttgcg caatgctcaa aaaattattg tgctcaaaaa cggtgaaaaa gcagaagaag
     1081 gaagtcacga agaactcatg aaaattgatg gtatttattg tgatatgtat aacaagcaat
     1141 tggtggaatc agacaatagc gcagacacta ttgcctaaaa cacttatttt atgaacattg
     1201 catagtatca ttgatttaaa taaataggaa acagtattat aattcgttga caccacttct
     1261 tgcctaccaa agaaaatcaa ccaattattt tgatttctca aaaattatcc taatattagc
     1321 gatacttatt atgataatta acgaagtcta tgatggaaaa cgagagtggc ggcgaggtgt
     1381 tttcaaaagc tataagagca ggtaaacgca cttatttctt cgacgtgaaa acaactaaag
     1441 gaaacgattt gtatttgaca gttaccgaaa gcaaaaaaat acaatacgac gggaaagaat
     1501 cgtatcaaaa acataaaatt ttcttgtaca aagaagactt tgaaaaattc aaagacggtt
     1561 tgcttgaagc gcttgaaaaa attgatgaat taaaagacga atacccatca acagagcgaa
     1621 gtgaccttaa ttttgaggat ttataaatca ctcatcacca attgttatca ccaatttttg
     1681 tttgaattta acaacataat tgcgtttaat ttctttaaaa ttcatccatt ccaatgccgg
     1741 attttgctgt tgtgttgcct ctaattcggg atattttagt gtaaaatcgc tcattgttgg
     1801 atggactaaa aaacaaaaat caccaccaat ggtatcgttt tcagaaatgg caatgcaata
     1861 catatcttta tcacgaggat tggtgtaaaa cgcttttact tgctcgccag gttgaacaac
     1921 tacccaccct tttgaaatcc aaccactgta ttgcgaacct ttgtcgtgaa acacataagc
     1981 cactttgtag gtctgacttt tattattctt aaaatacaac tgagcaaaca acccgttgca
     2041 aataattcca atgaacacga tgcttgtaaa aatcttttgc attgttgaca aatatttttt
     2101 gatacttcca aatatcgtaa cttttatcgg ttcgtttatt atatttgttg attaaataaa
     2161 aagttcaatg cattttaaac aagtagatac gtcggtagaa ctccctatca tggagcattt
     2221 ttattctgtt cagggtgaag ggttttattc tgggcgacct gcttatttta ttcgccttgc
     2281 tggttgcgac gttggttgtg tttggtgcga tgtgaaagaa agttgggatg tggaagagca
     2341 tccggttgtt caactagacg atttaatgca gcaaattaag gcaactgccg ccaactttgt
     2401 tgtcattact ggcggagaac ctgctttgta tgatttgcgt ggtttgattg accgactcaa
     2461 acaagccaac attaccattg ccatagaaac atcgggtaca agtgaattgg ttggtaatgt
     2521 tgattggtac tgcttttcgc ccaagaaatt taaaaaacca gtggatgaag cctatcaaaa
     2581 agcaagtgaa ctaaaaatta ttattttcaa taaatctgat tataaatggg ctgaagagca
     2641 cgcagcaaag gtgaatgcta actgtcagct gtatttgcaa cccgaatggt caaaacagga
     2701 acagtttttg ccacagatta ttgagtatgt gaaaaacaat cctaaatggc gcatttcact
     2761 acaaacacat aaattcatgc aaattccata attatgagac ttttaacgtt aattttttca
     2821 atagtattgg ttgctagttg cagcatttct caaaattatt cttcaacaaa taaaaaagcc
     2881 ataaaattgt ttgaagaagc aatgaaagca cctagtttgt ccattgaccc tgttagaaga
     2941 acgcctaatt ttagagcagg aattgaattg gccgaaaaag caattgcaaa agatcctcga
     3001 ttttgggagg cgcatttatt agcagccgaa ttgtatgaaa attattacga atatgcagca
     3061 gcaatcaaac attacgaagc agcacttgcc atcaacccca atcatgcacc atctggaagc
     3121 acctattttt atttaggaaa cagccaattt gcagttggcg attaccaagg agcgttgaaa
     3181 aaccttgaaa tttacaataa aaatcgcagt gccgatccac gtatgttgca agcagccaat
     3241 caaatcatgg acaactgtga atttgctttg gatgccatta aaaatccagc aaaatacaac
     3301 cctatcaacg ttggtccagg catcaatact gccgatccag aatatttccc aaccattacc
     3361 gttgatggaa aaacaatttt atttacacgc cgcattaaag acccgaactc tccagtaggc
     3421 atgcacgaag atttttttgt atccaactca gaagatggaa aaaaatggca aacagcagtt
     3481 cctatgccta aaaatattaa cacaaccaat aacgaaggag ctccaactat ttctgccgat
     3541 ggaagatcat tgatttttgt tgcttgctcc gatgaatctg gacgcaatta tggtccaaac
     3601 cgtgaaggtt ggggaagctg cgatttgttt tacactaaaa aattgggtac ccgttggtta
     3661 gatccaatca accttcctgg aaaagttaat acttctttgt gggaatcgca accatcgctt
     3721 tctgctgacg gtaaaacgtt gtattatgtt agaagagtga gcaaacctgg cgaatcacca
     3781 aatagcgata tttttgtaag ctacttgcaa gaagacggaa catggagcgc aggaaaaccg
     3841 ttgccaacca ctatcaacac taaattttta gaagaatcgg tgtacattca ccctgatggt
     3901 aaaacgttgt attttgcatc gcgaggtcat aaaaacttag gcggttcgga tttgtttgtt
     3961 tccaaaatgg acaaagacgg aaaatggggg ccagccgtaa atttaggata tcccatcaat
     4021 acgcaatatg atgaaaactc gttgttggta tctcctacag gtgaaattgc cttttttgca
     4081 tcagacagag aaggtggcta tggtgatttg gacatttatt attttgaatt gccggaggaa
     4141 ttacgtccaa ccaaaactgt ttattttgac ggaattgtat ttgatcaatc caacaagatg
     4201 ccattgcctg gtaagtttga gttaattgat attgaatcgg gagccgttgt gatgaactcc
     4261 gaggccgata aaattaatgg tcaattcatg gtttctttgc cagtgaataa agaatacgtt
     4321 ttgaatgtta gttacccggg ttatttattt ttctcgcaaa acttcaacat gacgttgcca
     4381 gacaaccaag aggctttcca catggatgtt ccgatggttt caattacagc agacattcct
     4441 gtttcgctta aaaatgtatt cttcgactta gcaaaagcaa ctttgcgtcc agaatcgcac
     4501 atagagttaa acaaattggt tgatttcatg gtgaaaaatc catcattaaa aattgaaatt
     4561 gctggtcaca ccgatacacg cggcgatgct aaagccaact taattttatc gcaagataga
     4621 gctaattcgg ttgttcaata tcttattgaa aaaggaatca atgccagccg tttggttgct
     4681 aaaggatatg gcgaaagcaa accgcttatt gccgatgctg aaatagaaaa aatggctaca
     4741 gagcaagaaa aagaaattgc ccaccaagaa aacagaagaa cagaatatag aataatacga
     4801 tgaaacacta cttaaaactt actcgtccgc tgaatttgct catcatttgc tttacaatgg
     4861 tgggagttcg gcactactta atggttacta atggtgaaac atatttttgg gaatggtctt
     4921 ttgccctttt ggtgctttct atggtactga ttgctggtgc tggaaacgtc atcaacgatt
     4981 attttgacgt ggaaaccgac cgcattaaca aacccgaaaa attaattgtt gggaaataca
     5041 tcacttccaa acaagcaatg aggtattaca ttgcactcaa tagcactgcc gttttgattg
     5101 ctttattttt atgttggcat ttggaaacat gggtttttgc agtttttcat ccgctattgg
     5161 tgttgttgct gtatttgtat agctattatt ttaagaaaac
//
LOCUS       DAOQVQ010000127         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17680_length_5180_cov_4.138732, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000127 DAOQVQ010000000
VERSION     DAOQVQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17680_length_5180_cov_4.138732"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..486
                     /locus_tag="PKN22_05220"
     CDS             61..486
                     /locus_tag="PKN22_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNS42139.1"
                     /translation="MDLIKKLLKNPSYGTGADFALLLIRIAAGGFMAFGHGWPKLMKI
                     MNGDMQFLDPIGLGPELSLILVFFAEFICGLLILIGLVPRLAAIVLVINMIVILFVVH
                     ISDPFADKELSYFYFVGYLAVLLAGAGKHSVQYMLSKNK"
     gene            502..2763
                     /gene="alr"
                     /locus_tag="PKN22_05225"
     CDS             502..2763
                     /gene="alr"
                     /locus_tag="PKN22_05225"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNS42140.1"
                     /translation="MKLNLTNGQLAKIVLSKNDCAQDTNGIQSVVYDTRRIGGTPNSV
                     FFALKGVHSNGHQYIKEAYQKGIRTFVVSEEIDQKQYVDATFLVVDDTFEALFRLAAH
                     HRSLLKGKMIVVSGKIGKTSVKEWLYHLLSPEKSVSRSPKSYNSNLGIALSLLEANPQ
                     SEVVIIEVKPHVELNPSKINALVKPDIGVLTTTDKGGEQFSDNYYELLFKGSSVLFHA
                     ERHRSILNLKSIVRFVASLPEELFSNEAKRKNVALAQAVALHLGIAPQKLKEKIKKLP
                     DLALRLETFEGNNGNFIINDLYNLDVDAFRNSLEFQQSIATTKKRAVILGAFHQQNEQ
                     QQEILDLIASFKPDYFYQLKENEPFNADLQNTVILIKGNASNYLLRIANLLKQRRHHT
                     HLRINLKSLRKNILAHKQALPANTKLMAMMKASGYGSGLDKIAQFVDSFGVDYFGVAF
                     VDEGVLIRNAGIKKPIMVMNTEEGNYEACIEHQLEPAIFDFQQLDDFISECIYQGVQG
                     YPIHLKMDTGMHRLGFELEQLDAVLEIVQSQPEVKIKSIYSHLAESDNLRDKRFTEHQ
                     IKLFSQAVQKLKNVLDYPFEQHILNSPGIENYPNVGFSMARLGIGMYGVSDNPAFKRK
                     LEPVLSWYSVVSQVRTVLAGQSVGYSRSHFCKTDTLVATIPVGYADGLRRSLSNGVGY
                     LTINGVKCPIIGRVCMDMVMVDATKAKAKVGDEVEIIGEHCSLEKMAELQQTIPYEVM
                     TSISERVHKIYTE"
     gene            2857..4047
                     /locus_tag="PKN22_05230"
     CDS             2857..4047
                     /locus_tag="PKN22_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS42141.1"
                     /translation="MEILLEEYQVKLARTSTKIKRYLFHQLDLSNRLIAIKGARGSGK
                     TTLLLQLAKLKLPQQTTLYVSLDHVYFYENKLYDLAKQFELYGGTHLLLDEVHKYPNW
                     SREIKLIYDNFPQLHVVFTSSSLLEIYKSESDLSRRAVSYHLKELSFREFILFETGKD
                     LNTFQLSELLSNHTALAQQIIDEIKVFPLFEKYLNIGAYPYYKENEVAFNQKLQNTIN
                     LIIEVDVNAVENINYDTIVKLKKLLKAVASSVPFTPNISKLSELVGLSRNSLVAALKI
                     LERAGLIHELHKNSTGIGVLTKPEKIYLNNTNLQYVLASDNTNIGNVRETFVLNQLKG
                     LHTVHLPPKGDFLIDDYIVLEVGGKNKTKKQISAVENAYVVKDQIEIGVGNTIPLWMF
                     GFLY"
     gene            4128..>5180
                     /locus_tag="PKN22_05235"
     CDS             4128..>5180
                     /locus_tag="PKN22_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNS42142.1"
                     /translation="MAQNSTQNILKQLLIRIVLLFLFWILLFDFQRIIFTFHNFDKFS
                     DVGFSTWIGAFFYSLRLDLATAGYLSFIPAIFLIIATIWQQKWARILFSTTLLVEVLV
                     CAFIHAGEVNAYPEWNHKLTGRVFMHLSHPDEVVRTADYGMTFWFIVYATLETLFAIF
                     IQKKFFRFENTNTKKGIALNLVYSVVFIPVTVGTSFLLARGGLQPIPINTDAAIFSNN
                     YAVNDLSVNSVYFFGKSYLLYNRTNIDEFIPKVSAEQAQQTLKELYDYPLEHSNYFLS
                     NKRPNIVFIVLESWTANAIGSITDVETATPNFDQLAKEGLLFTNLYGVASTSEIGNSA
                     IFSGYPGIPEVSITMQP"
BASE COUNT         1660 a          865 c          967 g         1688 t
ORIGIN      
        1 tatacatttg aatttttttt caattaaaat aaggttatat ttgttttaaa ataaacaact
       61 atggatttaa ttaaaaaatt attgaaaaac ccatcttatg ggacaggtgc cgattttgca
      121 cttttgttaa ttcgtattgc cgcaggtggc tttatggctt ttggtcacgg atggccaaaa
      181 ttgatgaaaa taatgaacgg cgacatgcaa tttctagacc ctattggttt gggaccagaa
      241 ttatctttaa tcttggtgtt ttttgctgag tttatttgtg gcttgcttat tttaattgga
      301 cttgtgccgc gcttggctgc cattgttttg gtcataaata tgattgtaat tttgtttgtg
      361 gtgcatattt ctgatccatt tgccgacaaa gaattgtctt acttctattt tgttggatat
      421 ttagcagtac ttttggcagg tgcaggaaaa cactctgtac aatatatgtt gtcaaaaaat
      481 aaatagtaaa aacaccacac tttgaaatta aatttaacca acggtcaatt agcaaaaatt
      541 gttttaagta aaaacgattg tgcccaagat acaaacggaa ttcaaagtgt tgtttacgat
      601 acaagaagaa ttggtggaac gcccaacagt gttttttttg cactcaaggg cgttcattcc
      661 aacggacatc aatacatcaa agaagcctat caaaaaggta ttcgcacatt tgttgttagc
      721 gaggaaattg atcaaaaaca atatgtagat gccacttttt tagtggtgga tgacaccttt
      781 gaagcattgt ttcgattggc tgcacaccac cgttctttgt tgaaaggaaa aatgatagtt
      841 gtttctggta aaattggtaa aaccagtgtg aaagaatggc tctatcattt gttaagtccc
      901 gaaaaatcag tttctcgaag tcccaaaagt tacaattcca acctcggaat agctttatcg
      961 ctactagagg caaatccaca atcggaggta gtaatcattg aagtgaaacc gcatgtagag
     1021 ctcaatcctt caaaaataaa tgcgttggta aaacccgata taggtgtgct cactacaact
     1081 gataaaggag gggagcagtt ttcggataat tactacgaat tattgttcaa aggaagttct
     1141 gttttattcc atgcagagcg ccaccgttca attttgaatt taaaatccat tgtacgtttt
     1201 gtagcctcgt tgcccgaaga attattttcc aacgaagcaa aacgaaaaaa tgttgctttg
     1261 gcacaagctg ttgccttgca tttaggaata gcacctcaaa aattaaaaga aaaaattaaa
     1321 aaattgcccg atttagccct tcgattagaa acatttgaag gtaataacgg taattttatc
     1381 atcaatgatt tgtataactt ggatgtggat gctttccgca attcactaga gtttcaacaa
     1441 tctattgcca caacaaagaa aagagcagtg attttaggtg cttttcacca gcaaaatgaa
     1501 cagcaacaag aaattttaga tctcatagcg tcttttaaac ccgattattt ctatcagcta
     1561 aaagaaaatg aaccttttaa tgctgattta caaaatacag ttattttaat caaaggaaat
     1621 gcatccaatt atttgttgcg cattgccaat ttactaaaac aacgtcgtca ccacacccat
     1681 ttacgcatta acttaaaatc gttgcgaaaa aatattttgg cacacaaaca agcattgcct
     1741 gccaatacca aactcatggc catgatgaaa gcatcgggtt atggctctgg attggacaaa
     1801 attgctcagt ttgtggatag ttttggagtt gattattttg gtgttgcctt tgttgacgaa
     1861 ggtgttttaa ttcgcaacgc tggtatcaaa aagcctatta tggtgatgaa taccgaagaa
     1921 ggcaattacg aagcctgcat tgaacatcaa ttagaaccag ccatttttga tttccaacaa
     1981 ttggatgatt ttatttcgga atgcatttac caaggtgtac aaggttatcc cattcactta
     2041 aaaatggaca ctggcatgca ccgtctagga tttgaattgg aacaattgga tgctgttttg
     2101 gaaattgtac aatcgcaacc cgaagtaaaa attaaaagca tatactcgca tttagcagaa
     2161 tcggataatt tacgcgacaa gcgttttacc gaacaccaaa taaaattgtt ttctcaggca
     2221 gttcaaaaac taaaaaatgt gttggattat ccgtttgaac aacatatttt aaattcgcca
     2281 ggtatagaaa actatccaaa tgtaggtttt tcaatggcac gtttgggaat tggaatgtat
     2341 ggtgtatccg acaatcctgc gtttaaacgc aaattagaac ccgtgttgag ttggtacagt
     2401 gttgtttcac aagtaagaac ggttttggct ggacaaagtg tgggttattc tcgctcgcat
     2461 ttttgcaaaa ctgatacact cgttgccacc attcctgttg gttatgcaga tggattgcgc
     2521 cgatcattaa gcaatggcgt tggttatctt actattaatg gtgttaaatg ccctattatt
     2581 ggtcgcgtgt gtatggatat ggttatggtg gatgctacaa aagcaaaagc aaaagtaggc
     2641 gacgaagtag agataattgg cgaacattgt tctcttgaaa aaatggccga attgcaacaa
     2701 accattcctt atgaagtaat gacctctatt tctgaaaggg ttcataaaat ttatacagaa
     2761 taactctgtt ttgctttatt gaatttacaa aataatagta aaaatgcttt ttatatttaa
     2821 caaaaggctc tatatttgtt gtgttaaaaa agcaaaatgg aaattttatt agaagaatat
     2881 caagtaaaat tggcgcgaac atctaccaaa attaaacggt atttgtttca tcaattggat
     2941 ttatccaacc gtttgattgc tataaaaggc gcgcgtggtt ctggtaaaac tacgcttttg
     3001 ttgcaactgg caaaattgaa attgccacag caaacaacct tgtatgttag cttagatcat
     3061 gtgtattttt atgaaaacaa attgtatgat ttggccaagc aatttgaatt gtatggtgga
     3121 acgcatttgt tgctggatga agtgcataaa tatccaaatt ggtcgcgcga gataaaactt
     3181 atttatgata attttccaca attgcacgtt gttttcacct cttcctcatt gctagaaatt
     3241 tacaaatctg agtcggattt aagtcgaagg gcagttagtt accacttaaa agaattatcg
     3301 tttcgagagt ttattctttt tgaaacaggc aaagatttaa acacttttca attgagtgaa
     3361 ttgttgagca atcacactgc attggcacag caaattattg atgagattaa agtatttcca
     3421 ttgtttgaaa aatacttaaa tatcggcgca tatccctatt acaaagaaaa tgaagttgct
     3481 tttaatcaaa aattgcaaaa taccattaat ctgataattg aagttgatgt taatgccgtt
     3541 gaaaatatta actacgacac cattgtaaaa ctcaaaaaat tactcaaagc agttgcttca
     3601 agtgtgccat tcacgcccaa tatttctaag ttaagtgaat tggtcggatt gtcgagaaat
     3661 agtttagtgg cagctctaaa aatacttgaa cgtgctggat tgattcatga attgcataaa
     3721 aattcaactg gaattggtgt attaacaaaa cccgaaaaaa tttacttaaa caacaccaat
     3781 ttacagtatg ttcttgcaag cgataatacc aatattggta atgtgcgcga aacttttgtg
     3841 ttgaatcaat tgaaaggttt gcacacagtc catttgccac caaaaggcga ttttttaatt
     3901 gatgattata ttgtactaga agttggtgga aaaaataaaa ctaaaaaaca aatttctgca
     3961 gtagaaaacg cttatgtggt aaaagatcaa atcgaaattg gtgttggtaa tacaataccg
     4021 ttgtggatgt ttggattttt atattgacct tcatccaaag aaacctactg ttattaacat
     4081 tttgcattaa aaataattcc taattttaga ccaattaaaa cgcaattgtg gcacaaaatt
     4141 ctactcaaaa catcttaaaa cagttattaa tacgcattgt attattgttt ttgttttgga
     4201 ttttattgtt tgattttcag cgtattattt tcacttttca caattttgat aaattctctg
     4261 atgtaggatt ttcaacttgg ataggtgcgt ttttctattc ccttcgatta gacttagcaa
     4321 ctgctggata tttgagcttt attcctgcca tttttttaat tattgcaaca atttggcaac
     4381 aaaaatgggc gcgtatttta ttttcaacta cgctattagt ggaagtgttg gtttgcgctt
     4441 tcatacatgc gggtgaggta aatgcttatc cagaatggaa ccacaagcta acggggcgtg
     4501 tattcatgca cttgtctcac cccgacgaag tggtgcgcac cgccgattat ggaatgactt
     4561 tttggtttat tgtctatgcc acattagaaa cgctgtttgc catttttatt caaaagaaat
     4621 ttttccgttt tgaaaataca aataccaaaa aaggaattgc tttaaacttg gtgtattctg
     4681 ttgtttttat ccctgtaaca gtgggaacaa gctttttgtt ggcgcgtgga ggcttgcaac
     4741 ccattccaat aaacacagat gcagccattt tttcaaacaa ttatgcggtc aatgatttat
     4801 cggtcaactc ggtctatttt ttcgggaaaa gttatttgct ttacaaccgc accaatattg
     4861 atgaatttat tccaaaagtg agtgccgaac aagcacaaca aacgcttaaa gaattatacg
     4921 attacccgtt ggaacattct aactattttt tatccaacaa gcgccccaat attgtgttta
     4981 ttgttttgga aagctggaca gccaacgcta ttggtagcat aaccgacgtt gaaacggcaa
     5041 cccctaattt tgatcaactg gccaaagaag gtttgttgtt taccaatctc tacggtgtgg
     5101 ctagtacctc agaaattggc aactcggcaa tttttagtgg ttatccagga attccagagg
     5161 tttcaattac catgcaaccc
//
LOCUS       DAOQVQ010000128         5179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17686_length_5179_cov_3.693794, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000128 DAOQVQ010000000
VERSION     DAOQVQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17686_length_5179_cov_3.693794"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /gene="lpxB"
                     /locus_tag="PKN22_05240"
     CDS             <1..779
                     /gene="lpxB"
                     /locus_tag="PKN22_05240"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNS42143.1"
                     /translation="PQVWAWKQNRVHTIKKVVDEMYVILPFEKDFYQKFDFNVTYVGH
                     PLLDAIENYKQSKSDDSVSFLQKNQLTEQPIIAVLPGSRKQEVSKKLPIMLEAVQSFT
                     NYQIVIAGAPSLDAAFYHPLIEKYKNVHLVHGQTYDLLAQAEAAVVTSGTATLETALF
                     QVPEVVCYIGSPISYIIAKQLIKIKFISLVNLIMDREVVKELIQNECNPSIIKAELSQ
                     LIKGGNKREAMLEDYQKLKEILGGGGASKKIAQSLLKTNE"
     gene            882..2006
                     /locus_tag="PKN22_05245"
     CDS             882..2006
                     /locus_tag="PKN22_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNS42144.1"
                     /translation="MQIGLFRDYKINRLVAAYNSGSYSIYADTTNLGSILPNEFVDVT
                     AEGNLLRLKVGVIDKGLFSTILLIQNNLGSSITLDPKSSQTIKSRKYEENISLTATKN
                     IITIVNLVDIDNYLSGVVESEGGGSQTIEYYKVQALISRTYALKYVGRHSKEGFSLCD
                     GVHCQAYHSMLRFTPIIREAVHSTTGSVLLDENNQLIDAYFHANCGGQTCEPDYVWNN
                     SVPYLTTFIDTFCIYTKQAKWEKRIAKPIWDNYFVSQFNYPIQDSIWKEKLYSFTQDI
                     RKAFYLTPALGIPLRDIRTKFGLKSTFFSTHLEGEEVVIEGRGYGHGVGLCQEGAMKM
                     SRLGYDYKQIAKFYFPGAKVIDLPESDFFKQLARKGPFKL"
     gene            2109..2489
                     /locus_tag="PKN22_05250"
     CDS             2109..2489
                     /locus_tag="PKN22_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNS42145.1"
                     /translation="MSETVRYSDAELEEFRVLIEQKLAEAKEHLKMLKGTLSHSDDHG
                     TNDTGRTFNMMEDGSDTLSREEVAQLAARQEKFIQNLQSALIRIENKTYGICRVTGKK
                     IQKERLMLVPHATLSIEAKNNEQK"
     gene            2476..3162
                     /locus_tag="PKN22_05255"
     CDS             2476..3162
                     /locus_tag="PKN22_05255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNS42146.1"
                     /translation="MNKNNSSAKRNILIVGGMIALILLLDQLLKIYVKTHFQLGATHP
                     IIGDWFVMEYIENRGMAFGTSFGNKAWHKLALSIFRIIAVTALIYYWIKKAKEGMKLT
                     FLIPLGLIIAGAMGNLLDSMYFDYYFPYDPCFPYNIQEGSGIWTDCNIWGFTERIETK
                     PRGFLFGNVVDMFKFDATWPKSIPFVGGTAVFPAIWNLADAAITVGIIIILIWNKTFF
                     PKTTTPKSDN"
     gene            complement(3146..3958)
                     /locus_tag="PKN22_05260"
     CDS             complement(3146..3958)
                     /locus_tag="PKN22_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42147.1"
                     /translation="MATFKKIVYFLDSSNLNISFSGAVLTYGIVSHFLMEHALEYALI
                     VFFLTFSIYNLQRLVDKRGFALSEPPLWGKYIYLPLILSIISLLIAFTIGLKLFYFSW
                     WLLLLSVTFAFISMWYTTPIFGLKLREIPGFKIVITALTWGYASAFFPLVNEGLFIGT
                     SGLFMLLIYNYFVAIIIPFDIRDMPIDNLKNSTLPQLLGVEYSKLVGIAFLLIFIIGN
                     LYLGFMEPDNLLFYLAVLVQLLFLVFANQKRPFYYFTLIDFSIVLLGISYLI"
     gene            4026..4946
                     /gene="mreC"
                     /locus_tag="PKN22_05265"
     CDS             4026..4946
                     /gene="mreC"
                     /locus_tag="PKN22_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNS42148.1"
                     /translation="MNFKFHFLINNLFFYLSHFVSLYYLCKLMRNLIAFLRRFQVFLV
                     FALLQVVALTLYFSFFFYPRSQYLTTAAGFNAKIWELSNGITEHLKLKKTNVALQQKN
                     KELMEKIPSSYTRINNSTVKVDDTIYQQKYEFIPGEIINSTVTNRNNYFTINIGTLNG
                     VERGLGVISDNGVVGVIHKCSDHYSIVKSVLTENINISVVVDKVGLFGLLKWDGVDPR
                     RGTVAGISNDIRIKKWSTIVTREGTGLFPKGIPVGKVEKLKKVEGEAFWDVSIVFSED
                     YRKLQKIYVVKNILAQEQEKLESLIPQNKN"
BASE COUNT         1828 a          816 c          906 g         1629 t
ORIGIN      
        1 cgccacaagt gtgggcttgg aaacaaaaca gagtgcatac tatcaaaaaa gtggtggatg
       61 aaatgtatgt cattctcccg tttgaaaaag atttttacca aaaatttgat tttaacgtaa
      121 cctacgtagg tcaccccttg ttggatgcaa tagaaaacta caaacaatca aaaagcgatg
      181 attctgtttc atttcttcaa aaaaatcagt tgactgagca accaattatt gctgttttgc
      241 ctggaagtag aaagcaagaa gtgtctaaaa aacttccaat catgttagag gcagttcaat
      301 catttaccaa ttatcaaatt gtaattgccg gagcgccaag tttagatgcc gcgttttacc
      361 atcctttaat tgaaaaatat aaaaatgtgc acttggttca cggtcaaacc tatgatttac
      421 ttgcccaagc cgaagctgca gttgtaacat cgggaacagc taccttagaa acggctcttt
      481 ttcaagttcc agaagtagtg tgttatatcg gttcgccaat ttcatacatc attgctaagc
      541 aattgataaa aattaaattc atctctttgg tgaacctcat tatggataga gaagtggtga
      601 aagaattgat tcaaaacgaa tgcaatccat caatcattaa agctgagttg agtcaactaa
      661 tcaaaggtgg aaacaaacga gaggcaatgc ttgaagatta ccaaaaatta aaagaaatct
      721 taggcggagg aggtgcaagc aaaaaaattg cacaatcctt gttgaaaact aatgagtgaa
      781 gcataaaatc gtatgtttca cctgattaaa tttgtaaagt gaagcaatta attctgatag
      841 ctattttact gaattgttct ttttttgcat ttagccaaca gatgcaaata ggactttttc
      901 gtgattataa aatcaatcga ttggttgccg cctataattc tggaagctat tccatatatg
      961 ccgatacaac caatttaggt tccattttac ccaatgaatt tgttgatgta acggctgaag
     1021 gaaatttatt gcgcttaaaa gttggtgtca ttgacaaagg tttgttttct accattttat
     1081 taatccaaaa caatttaggt tcgtccataa cccttgaccc caaatctagt caaacaataa
     1141 aatcaagaaa atacgaagaa aatatatcgc taacagcaac taaaaatatc attaccattg
     1201 tcaatttagt ggatattgat aattatttat cgggagttgt agaatccgaa ggaggaggta
     1261 gccaaaccat cgaatattac aaagttcaag cgctgattag tcgcacctat gcattaaaat
     1321 acgttggtcg ccattccaaa gaaggttttt cactctgcga tggcgttcat tgccaagcct
     1381 atcacagcat gttgcgcttt acacccatca tacgcgaagc cgttcattcc actacaggta
     1441 gtgtgttgtt ggacgaaaac aatcaattaa tcgatgcgta ttttcatgcc aattgtggcg
     1501 ggcaaacgtg tgaacccgat tatgtgtgga acaattcagt gccctattta actacattta
     1561 ttgatacatt ttgtatttat accaaacaag caaagtggga aaaaagaatt gcaaaaccaa
     1621 tttgggacaa ttatttcgtt tcgcagttta actatcccat ccaagatagc atttggaaag
     1681 aaaaattgta ttcatttaca caagatattc gaaaagcatt ttatttgacg ccagctttgg
     1741 gaattccttt gagggatatt cgaacaaaat tcgggttaaa atcaacgttt ttttctacgc
     1801 atttggaagg ggaagaagtg gttattgaag ggagaggcta tggtcatggt gtaggtttgt
     1861 gtcaagaagg agcgatgaaa atgtcgcgtt taggttacga ttacaaacaa attgcaaagt
     1921 tttattttcc aggcgctaaa gttatcgatt taccagaaag tgattttttc aaacaattgg
     1981 caagaaaagg tccttttaaa ctataaaaat ccttaattaa accaaatttc gtattgctgt
     2041 ttcattataa atgttatttt tgctgactta taaaattgaa agatataatc aaaactaaat
     2101 acaaagctat gagtgaaaca gtaagatatt cagatgcaga attagaagaa ttcagagtgt
     2161 taattgaaca aaaattggca gaagcgaaag aacatttaaa aatgttgaaa ggaaccttgt
     2221 ctcactcaga tgatcacgga acaaacgata ccggacgtac cttcaacatg atggaagatg
     2281 gttctgatac cttatcgcgc gaagaagtag ctcaattggc tgctcgtcaa gaaaaattta
     2341 ttcagaattt acaaagtgct ttgattcgaa ttgagaataa aacctatgga atttgtagag
     2401 taactggcaa aaaaattcaa aaagaacgtt tgatgttggt acctcacgca actttaagta
     2461 tcgaagcaaa aaacaatgaa caaaaataat tcttcggcta aacgcaatat tttgattgtt
     2521 ggtggaatga ttgcactcat tctactgctc gatcaattat taaaaattta tgtaaaaacg
     2581 catttccaat tgggtgcaac tcatcctatt attggcgatt ggtttgtaat ggaatacatt
     2641 gaaaaccgag ggatggcttt cggaacttct ttcggaaaca aagcgtggca taaattagcg
     2701 ttgagcattt ttagaattat tgcagttact gcgttaattt attattggat aaaaaaagcc
     2761 aaagaaggaa tgaaattaac ctttctaatt cctttaggac taattattgc tggtgcaatg
     2821 ggaaatttac tcgactcaat gtatttcgac tattatttcc cttatgaccc atgtttccca
     2881 tacaatatac aagaaggttc aggtatttgg acagattgta atatttgggg ctttacagaa
     2941 agaattgaaa caaaaccaag aggattttta tttggaaatg tggtagatat gttcaaattt
     3001 gacgccactt ggccaaaaag catccctttt gttggtggaa ctgctgtttt tcctgctata
     3061 tggaaccttg cagatgcagc aattactgta ggaatcatca ttattttaat ttggaataaa
     3121 accttttttc caaaaacaac aacacctaaa tcagataact aattcccaat aaaacgattg
     3181 aaaaatcaat caaagtgaaa tagtaaaatg gacgtttttg atttgcaaaa actaaaaaga
     3241 gcaattgcac taatacggcc aaataaaaca aaagattatc aggttccata aaacctaaat
     3301 acagattgcc aattataaaa attaatagaa aagcaattcc aaccaattta gaatattcaa
     3361 cacctaacaa ttgaggtaga gtgctgttct ttagattgtc tatcggcata tcgcgaatgt
     3421 caaacggaat aatgatggca acaaagtagt tgtaaatcaa caacataaat aatcctgaag
     3481 ttccaataaa caaaccttca tttaccaatg ggaaaaatgc actagcatat ccccaagtaa
     3541 gcgctgtaat cacaatttta aagccgggta tttcacgcaa ttttaaacca aaaatgggtg
     3601 ttgtgtacca catacttata aatgcaaatg tcactgaaag taacagtaac caccaactga
     3661 aataaaataa ttttaaccca attgtaaacg caattaataa agatatgatg ctcaaaatta
     3721 agggaagata aatgtatttt ccccataaag gtggttctga aagtgcaaat ccacgtttat
     3781 ctactaatcg ttgtaaatta taaattgaaa aagttagaaa aaatacaatt aatgcatatt
     3841 ccaaagcatg ttccatcaaa aaatgcgaaa ctattccgta ggtcaaaaca gcacctgaaa
     3901 agcttatatt gagattggag gagtcaagaa aatatactat ttttttgaat gttgccatgt
     3961 actttaactt tcacaaaaat aaacataact aatagtttca ttgaatttct tagacataac
     4021 ttcggttgaa ttttaaattt cattttttaa taaacaattt atttttttat ttatctcatt
     4081 ttgttagcct ttattatctt tgcaagctta tgcgtaatct cattgctttt ttaagacgtt
     4141 ttcaagtatt tttggtattt gctttattgc aagtggtggc gcttacattg tatttttctt
     4201 ttttctttta tccacgatca caatacctta caactgcagc tggatttaat gcaaaaattt
     4261 gggaattgag taatggaata acagaacatc tcaaattaaa gaaaaccaat gttgcattgc
     4321 agcaaaagaa taaagaatta atggaaaaaa taccatcaag ttacactcga atcaataatt
     4381 ccacagtaaa agttgatgat actatttacc aacaaaaata tgagtttata ccaggagaaa
     4441 tcataaattc aactgttaca aatagaaaca attatttcac aatcaacatc ggaacactaa
     4501 atggcgttga acgcggttta ggtgtaattt ctgataatgg cgtagtaggg gtgattcata
     4561 aatgtagcga ccattattct attgttaagt ctgtacttac tgaaaacatt aatattagtg
     4621 tggttgttga taaagtgggt ttgtttggtt tacttaaatg ggatggagtt gaccctagaa
     4681 gaggtactgt tgctggaatt tcaaatgata tccgtattaa aaaatggtca actattgtaa
     4741 cgagagaagg aactgggctt tttccaaaag gtattccagt tggtaaagtt gaaaaattga
     4801 aaaaagttga aggtgaagca ttttgggatg tttccatcgt gttttctgaa gattatcgaa
     4861 agttgcaaaa aatatatgtt gttaaaaata ttttggcgca agaacaagaa aaacttgaat
     4921 cactaatacc tcaaaacaaa aattaatgaa actcatcatt acacatagta taagatttgt
     4981 attatttgtt tttattcaag tattagtctt taaccaattg gagataggat ggggtattca
     5041 tcctatggtg tatcctttgt ttatcatgct gcttcctttt ggaatttctt ccattacaat
     5101 gttttttata gcatttttca tgggcttctt tattgatatt ctttcactat ttatgttatc
     5161 taaacacttt tcatgtata
//
LOCUS       DAOQVQ010000129         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17791_length_5160_cov_3.880901, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000129 DAOQVQ010000000
VERSION     DAOQVQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17791_length_5160_cov_3.880901"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1917
                     /locus_tag="PKN22_05270"
     CDS             <1..1917
                     /locus_tag="PKN22_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNS42149.1"
                     /translation="ELFIQTVKAIAPTFGGINLEDIKAPEAFEIERRLKEELDIPLMH
                     DDQHGTAIISAAALLNALEIAKKKIGKVKIVVSGAGASALSCARLYKSLGAKYENIFM
                     YDSKGLIHAGRTDLDHNKQEFAVHKKDVAFEDAFKKADVFLGLSRAGLVNKDMIKAMA
                     KDPIVFALANPDPEISYKDATSARKDIIMATGRSDHPNQVNNVLGFPYIFRGALDVRA
                     TTINEAMKLAAVHAIASLAKETIPEEVITAYNEKNISFGRESIIPKPLDPRLIYYVAP
                     AVARAAMESGVAKNPIKDWAAYELELKKRLGLENKLIRNLTDKAQNNPKRVVFAEADN
                     IKILKAAQTSAEEGIAYPILLGNKEIINNLIHEYSLDFHDVTIIDPKSDDEKERRMTY
                     GKKFFEKRKRKGVTEFEAIQTMRERNHFGAMMVDMGDADAMISGLTRNYRSVVKPVIQ
                     TIGLEKDVKSVAGLYILNTKRGPLFLADTTINIDPTAEEIAEITANVAKTMRKFKIIP
                     RIALLSYSNFGSSYGVDPEKMAKATKILHENYPNLIVDGEVQANFALNSELMNEKFSF
                     SNLANKNVNTLIFPNLSSGNIAYKLLQETTGGDAVGPILVGLKKSVHVLQMGATVREI
                     INMVKVAVVDASTK"
     gene            1923..2498
                     /gene="ruvA"
                     /locus_tag="PKN22_05275"
     CDS             1923..2498
                     /gene="ruvA"
                     /locus_tag="PKN22_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNS42150.1"
                     /translation="MIAHLNGKLIEKNPTNIIVECNGVGYFVNISLNTFSALGSNEAV
                     KIYTKMIVREDAHLLYGFATTDERSMFEHLISVSGIGPSTGMIMLSSLTPEEVANAIQ
                     TEDVRTIQSIKGIGAKTAQRVIIDLKDKVVKMNFSSENIFTQNNTNRFDALTALISLG
                     FDKKLAEKAIDKVSQMDDSVEKIIKEALKIL"
     gene            2536..>5160
                     /gene="sprA"
                     /locus_tag="PKN22_05280"
     CDS             2536..>5160
                     /gene="sprA"
                     /locus_tag="PKN22_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNS42151.1"
                     /translation="MRNCARICFLILTCVCATTIYAQTDSLRYPITPTYDPTQSKKQS
                     FDFGDPSSVQQTIVYDPVTGTYVFKETIGTSGIDFRPPSMMTLEEYLEYDRKKSISDN
                     WKDKIDQQTEDSQPFDIPIKIKNKLFKNFFGSDQITIKPQGTVEIGLGVNSSRYDNPL
                     LPVKQRRITRFDFNQNINFNVVGQIGTRVKLGLSYNTQANFEFENVQKLEYTGDEDQI
                     VQSIALGNVSLDLPTTLIPSSKTLFGASTKLKFGRTTLDLIAASSKGQRKEIKVEGGA
                     QKQTFELPADQYEANRHYFLNYYFRDNYDAALKELPIVNSGINISRLEVWITNKTNAT
                     ENTRNIVAFTDMGEGMPDYLEGTPGTLSNNPLPDNNANGLYAWATAQPGIRDFTAAVP
                     TLSSQVVAPGPFTQSIHYEKVESARKLNESEFQYNAQLGFISLNMPLNNDEVLAVAYE
                     YTYRGQTYRVGELSTDGVTGTNALVLKLLKSTMTYPKNKLWDLMMKNVYSIGAYQVSQ
                     EGFRLNVLYNNPENSLYIPFFPQDGLDDVQIVTMLEMDKLNQVGQPFSDGVFDYVPMN
                     LNGNRFENGGTINARNGRIYFSTVEPFGKVLAQKLLAEGIPQTTVDRLAFTELYDSTK
                     TAAQQIPSKNRFLFKGEYKSSVSSDIPLNSLNVPEGAVSVTAGGIKLTEGVDYTVDYN
                     LGRVKILNDGLLASNQEIKVSVESNSAFGFQAKSLFGARVAHRFSQNFNVGATWMRMM
                     ERPVTQKIDIGSEPYKNNIVGFDIQFRTELPFLTKLIDKLPVISTKEKSFLSFSGEFA
                     HLIPGQPRAISKAGISYIDDFEGAQSTIDLTSVNAWYLASVPTGQPSLFPEASSLGLA
                     KTAKRSKISWYRIDDIFYR"
BASE COUNT         1794 a          896 c          990 g         1480 t
ORIGIN      
        1 gaattgttta tccaaacagt aaaagccatt gcgccaactt ttggaggaat caatttagaa
       61 gatatcaaag ctcccgaagc atttgaaatt gaacgtagat taaaagaaga attggacatt
      121 ccgttgatgc acgatgatca acacgggaca gccatcattt ctgcagctgc cttgttaaat
      181 gcattggaaa ttgctaaaaa gaaaattgga aaagtaaaaa ttgtagtaag tggagccggt
      241 gcttctgcac tttcttgtgc tcgcctatac aaatcgttgg gtgctaaata tgaaaatatt
      301 ttcatgtatg attcaaaagg attgatacat gccggtagaa cagatttaga ccacaataag
      361 caagaatttg cggtacataa aaaagatgtt gcgtttgaag atgccttcaa aaaagcagat
      421 gtgtttttag gattatcgcg tgccggactt gtgaacaagg acatgattaa agcaatggcg
      481 aaagatccta ttgtttttgc tttggcaaat cctgatccag aaatttcgta caaagacgca
      541 accagtgcac gaaaagatat tatcatggct acaggtcgct ccgaccatcc taatcaagtg
      601 aacaatgtgc ttgggttccc ctacatattt agaggagcat tggatgtaag agcaactacc
      661 attaatgaag caatgaagtt ggctgccgtt catgccattg cgtcattggc caaagaaacc
      721 attccagaag aggtgattac agcctataac gaaaaaaata tttcgtttgg tcgcgaatct
      781 attattccaa aaccattaga ccctcgcttg atttattacg ttgcgccagc tgttgcacgt
      841 gcagcaatgg aatcaggtgt agctaaaaac ccaataaaag attgggcagc ctatgaattg
      901 gaattaaaaa aacgtttagg attagaaaat aaattaatca gaaatctgac cgataaagca
      961 caaaacaatc caaaacgtgt ggtatttgct gaagcagata atattaaaat tttaaaagcc
     1021 gcacaaacgt cagccgaaga aggaattgct tatccaattt tattggggaa taaggaaatt
     1081 atcaataact taattcatga atacagccta gattttcatg atgtaacgat aatcgacccg
     1141 aaatcggatg atgaaaaaga acgaagaatg acttacggga aaaaattctt cgaaaagaga
     1201 aaacgcaaag gagttaccga atttgaagcc attcaaacca tgcgtgaacg taaccatttt
     1261 ggcgcaatga tggtggatat gggcgatgca gatgctatga tttctggttt aacaagaaat
     1321 taccgctctg ttgtgaagcc tgtaattcaa acaattggtt tagaaaaaga tgtaaaatct
     1381 gtagccggtt tgtatatttt gaatactaaa agaggaccac ttttcttggc agatacaacc
     1441 atcaatattg atccaacagc agaagaaatt gcagaaataa cggcaaatgt tgctaaaaca
     1501 atgcgtaagt ttaaaataat tccacgtatt gccttgcttt cctactctaa ttttggttct
     1561 tcgtatggcg ttgaccctga aaaaatggct aaagcaacaa aaatattgca cgaaaactat
     1621 ccgaacttaa ttgtagatgg agaagtgcaa gctaattttg cattgaacag tgaattgatg
     1681 aatgaaaaat tcagcttttc taatttagca aataaaaatg ttaatacctt gatattcccg
     1741 aatttatctt caggaaatat tgcctataaa ttactacaag aaacaactgg tggcgatgct
     1801 gtaggaccaa ttttggtggg attaaaaaaa tcggttcatg ttttacaaat gggagcaacc
     1861 gttcgtgaaa ttataaacat ggttaaagtt gctgttgttg atgcgagtac taaataaccc
     1921 ctatgattgc acatttaaac ggaaaactaa tagaaaaaaa tccaactaac atcattgtag
     1981 aatgcaatgg tgttggttat tttgtgaata tatcactgaa tacattttcg gcacttggaa
     2041 gcaatgaggc tgtaaaaatt tacaccaaaa tgattgtgcg cgaagatgca catctacttt
     2101 atggatttgc aaccaccgat gaacgatcga tgtttgaaca tttaatttct gtttcaggaa
     2161 ttggtccaag cacaggtatg attatgctct cttcgctaac ccccgaagaa gtagccaatg
     2221 ccatccaaac agaagatgtg cgaaccattc aaagtataaa aggtattgga gcaaaaactg
     2281 ctcaacgagt aatcattgat ttgaaagata aagttgttaa aatgaacttt tcttctgaaa
     2341 atattttcac tcagaacaat acaaacagat ttgatgcgtt aacagcatta atttcgcttg
     2401 gttttgacaa gaaattggct gaaaaagcga ttgataaagt gagtcagatg gatgactccg
     2461 tggaaaagat aattaaagaa gcattaaaaa ttttataata tatacgtgtt gaacaaaatc
     2521 acacatatcc tacttatgag gaattgcgca agaatttgtt ttcttattct tacttgtgta
     2581 tgtgcaacta ctatttacgc acaaacagat agtttaagat accctattac tccaacatac
     2641 gacccaacgc aaagtaaaaa acaaagcttt gattttggcg acccatcatc tgtgcaacag
     2701 accattgtgt atgaccctgt tacagggacc tatgttttta aagaaacaat tggtacttct
     2761 ggaatagatt ttcgcccacc ttccatgatg accttggaag aatatttgga atacgacaga
     2821 aaaaaatcca taagtgataa ctggaaagat aaaattgacc aacaaacaga ggatagtcaa
     2881 ccatttgata ttccaataaa aataaaaaat aaacttttta aaaacttttt tggttccgac
     2941 caaattacta tcaaaccaca aggtacggtt gaaattggtt taggcgttaa ctcttcgcgc
     3001 tacgataatc cgcttctacc tgtaaaacaa agacgcatca cacgttttga tttcaaccaa
     3061 aacattaatt ttaatgtggt aggacaaatt ggtacacgtg taaaacttgg tttgagctac
     3121 aacacacaag ctaattttga atttgaaaat gttcaaaaat tagaatatac gggtgatgaa
     3181 gaccaaattg ttcaaagcat tgcacttgga aacgtatcgt tggatttacc aacaacatta
     3241 attccaagct ctaaaacatt gtttggtgct agtacgaaat taaaattcgg aagaacaacc
     3301 ctcgacctaa ttgctgcatc ttctaaaggg caacgtaaag aaattaaagt agaaggaggg
     3361 gctcaaaaac aaacatttga acttccagcc gatcaatatg aagctaatcg ccactatttc
     3421 ttaaactatt attttagaga taattacgat gcagctttaa aagaacttcc aattgtcaat
     3481 tctggtatca atatttcccg attagaagtt tggataacca ataaaacaaa cgcaacagaa
     3541 aatacaagaa acattgttgc atttaccgat atgggggagg gaatgccaga ttacttagaa
     3601 gggacaccag gaaccttgtc caataaccca ttgccagaca ataacgctaa tggtttgtat
     3661 gcatgggcaa cagctcagcc cggaattaga gattttacag ctgctgtacc aacattgagc
     3721 tcacaagtgg tggcgccagg tccatttacg caatctatac attacgaaaa agttgaaagt
     3781 gctagaaaat taaatgaatc tgaatttcaa tacaacgcgc aattaggatt tatttcccta
     3841 aatatgcctc tcaataacga tgaggttttg gcagttgctt atgaatatac ttacagagga
     3901 caaacctata gagttggtga actttcaaca gatggggtaa ccggaacaaa tgctttggtg
     3961 ttgaaattgt tgaaatcaac aatgacttat ccaaaaaata agttgtggga tttgatgatg
     4021 aaaaacgttt attcaatcgg agcatatcaa gttagtcaag aaggttttcg tttgaatgtt
     4081 ttatacaaca accctgaaaa ctcattgtac atcccattct ttcctcaaga cggattggac
     4141 gacgtgcaga ttgttaccat gttagaaatg gataaactca accaagtggg gcaaccgttc
     4201 tcggatggag tttttgacta tgtacctatg aatttgaatg gcaaccgatt tgaaaatggt
     4261 ggtactatca atgctcgaaa tggtcgtatt tatttcagca cagttgaacc ttttggtaaa
     4321 gtattagcgc aaaaattatt ggcagaaggt attcctcaaa caactgttga ccgtcttgcc
     4381 tttacagaat tatacgactc aacaaaaaca gctgcccagc aaattccatc caaaaacaga
     4441 ttcttgttta aaggagaata caaatcatct gtttctagtg atattccgct aaattcattg
     4501 aacgtgccag aaggtgccgt gtcggttact gcaggtggta tcaaattaac cgaaggtgtt
     4561 gattatacag ttgattacaa cttaggacgt gttaaaattt tgaacgacgg tttgctagct
     4621 tctaaccaag aaattaaagt atcggttgaa agtaactcgg cttttggttt tcaagcaaaa
     4681 tctttgtttg gagcgcgcgt ggcacatcgt ttcagtcaaa attttaatgt aggagctacg
     4741 tggatgcgca tgatggaacg tcctgtaact caaaaaattg atataggaag tgagccgtat
     4801 aaaaataaca ttgttggctt tgatattcaa ttccgtacag aattaccgtt cttaaccaaa
     4861 ttgattgata aattacctgt tatttccaca aaagaaaaat cgtttttatc tttttctggt
     4921 gaatttgcac acttaattcc gggtcaacca agagctatta gcaaagccgg aatttcttat
     4981 attgatgatt ttgaaggagc gcaaagcacc attgacttaa caagtgttaa tgcgtggtat
     5041 ttagcatccg taccaacagg acagccctct ttatttccag aagcttcaag tttaggattg
     5101 gctaaaactg caaaacgatc taaaatttca tggtaccgca ttgatgatat tttctaccgt
//
LOCUS       DAOQVQ010000130         5132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17907_length_5132_cov_3.783140, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000130 DAOQVQ010000000
VERSION     DAOQVQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17907_length_5132_cov_3.783140"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKN22_05285"
     CDS             complement(<1..302)
                     /locus_tag="PKN22_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42152.1"
                     /translation="MDKKTVIGLVLIGTIFSVFTIFNQPTAEERAQMQAELKKEQKLK
                     DEKQKLAQEAEKTVKATPSTTGDSTATTTKIASKKAVATDSLVFLETSKYKVAFNT"
     gene            complement(318..1928)
                     /locus_tag="PKN22_05290"
     CDS             complement(318..1928)
                     /locus_tag="PKN22_05290"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNS42153.1"
                     /translation="MSNSTKYVFVTGGVTSSLGKGIISASLAKLLQARGYSTTIQKLD
                     PYINIDPGTLNPYEHGECFVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQSVI
                     EKERRGDYLGKTVQVIPHITDEIKERIQILGKNGEFDIVITEIGGTIGDIESLPYVEA
                     VRQLMWEEENNCIVIHLTLVPYLAAAGELKTKPTQHSVKQLLEFGVQPDILVCRTEHE
                     LDLNIRRKIAKFCNVSMNAVIESRDASTIYDVPLLMQEEELDKVVLEKLQLSTRTTPN
                     LDNWKNFLTDLKNPITEVNIGLIGKYVELKDSYKSISEAFIHAGVANKCKVNVKWIHS
                     EKITDENLTDELKDLDGILVAPGFGSRGIAGKIQSIKYARENNVPFFGICLGMQCAVI
                     EYARNVLGWSDAHSTEIAEDSTHPVIDIMEEQKKVSNKGGTMRLGAYKCHLKANSKAY
                     IAYNTDVISERHRHRFEFNNSYLQDFENAGMIATGKNPDTGLVEIVEIPSHPWFVGAQ
                     FHPEYKSTVACPHPLFVAFIKAAIENKK"
     gene            complement(2028..4286)
                     /locus_tag="PKN22_05295"
     CDS             complement(2028..4286)
                     /locus_tag="PKN22_05295"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNS42154.1"
                     /translation="MAKASFDISQIDLEKERLEILRAFKQLMRHVKNRSRKDTEMIRK
                     AFDMAVEAHKDMRRASGEPYIYHPIAVARICVEEMGLGPTAIICALLHDTVEDTYITL
                     QDIEDVFGSKVRLIIDGLTKIPEVFDENASIQAENFRKMVLTISEDLRVVLIKLADRL
                     HNMRTLDSMREDKQLKIASETKFLYAPLAHRLGLYSIKSELEDLSFKYTEKEVYKQIE
                     TALKSSQEVRNRFIRRFVLPIKESMTNEGFQFEIKARTKSISSIWNKMQSKGIPFEEV
                     YDVFAIRIILDSPLELEKADCWKVYSIVTDFYQPSPDRLRDWISTPRANGYESLHTTV
                     MSPQGKWVEVQIRTRRMDEIAEKGLAAHYKYKESKKDDSKLDRWIAEIRSLLENPDLN
                     AMDFINEFKLNLFNDEIYVFTPKGELRVLPTGATVLDFAYDIHTRIGDTCIGAKVNNK
                     LMPLSYQLQSGDQLEIITSSKQKPNEDWLSMVVTARAKQKIKANLNEERKQIAEDGKE
                     ILMRKFKQHNILFVSENITFLEKAYQLPSATELYFRIAKGKMDLTKLREFDNDNGYLS
                     HKKSEDTSKRIRKKDNIQASVNKKSDTLIIGENFKNIHYTLAKCCSPIPGDKVFGFIT
                     INDGVKIHRTNCPNAENLMSKMAYRCINARWKDQDIEEREVTIKIYGFDRMGIVNKLT
                     GIISNQQNVNMRAISFETEGGLFEGKVRVMVLDTKHLELLKNKIEEVEGVQRVIRWDE
                     ENNEPEDYVEED"
     gene            complement(4440..>5132)
                     /locus_tag="PKN22_05300"
     CDS             complement(4440..>5132)
                     /locus_tag="PKN22_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNS42155.1"
                     /translation="ENPIYAPCQRLDFELETAFITYDGKPLGDSITTNEAEDYIFGMA
                     LFNDWSARDIQTWEYVPLGPFLAKNFASSLSPWIVTLDALQPFKVDGPEQDPAVLSYL
                     EFTGKKSYDINLQVYLQPENSTETLICKSNFKYMYWNMAQQLAHHTVNGCNVRCGDLM
                     GSGTISGPTPDSYGSMLELAWKGTKPLKMNDGTERKFIQDNDTVIMRGYSEKDGIRIG
                     FGEVSSKVLPAK"
BASE COUNT         1442 a          994 c          908 g         1788 t
ORIGIN      
        1 ttggtgttga aagcaacttt gtatttgctt gtttccaaga aaaccaacga atctgttgca
       61 accgctttct tagaagcaat tttagttgtt gttgctgtag aatctccagt agttgatgga
      121 gttgccttta ctgttttttc agcttcttga gctaattttt gtttttcgtc ttttaatttc
      181 tgttcttttt ttaattcagc ttgcatttga gcacgctctt ctgcagttgg ttgattaaaa
      241 atggtaaaaa cagaaaaaat tgttccgatt aatacgagtc cgatgactgt ttttttatcc
      301 atgtattgtt ttcttaatta ttttttattt tcaattgccg ctttaataaa cgccacaaaa
      361 agtgggtgag ggcaagctac tgtactttta tattctgggt ggaattgtgc accaacaaac
      421 caaggatgtg atgggatttc aacaatttca acaaggccag tatctggatt ttttcctgtt
      481 gcaatcatgc cagcattttc aaaatcttgc aagtaactgt tgttaaactc aaaacggtgt
      541 ctgtggcgct cactgattac atctgtatta taagcgatat atgcttttga attggctttt
      601 aaatgacatt tgtaagctcc caaacgcata gttccacctt tgttggacac tttcttttgt
      661 tcttccatga tgtcaattac aggatgcgtg ctatcttctg caatctcagt tgaatgtgcg
      721 tcagaccaac ccaaaacgtt gcgagcatat tcaataaccg cacactgcat tcctaaacaa
      781 attccaaaga aagggacatt gttttcacgt gcatatttga tggattgaat ttttccggca
      841 attccacgag aaccaaatcc tggagcaacc aaaattccgt ccaagtcttt taattcatcc
      901 gttaagttct catccgttat cttctctgaa tgaatccatt ttacatttac tttgcattta
      961 ttggcaacac ctgcatgaat aaatgcctct gaaattgatt tataagaatc ttttaattct
     1021 acgtattttc caatcaaacc aatgttgact tctgtaattg gattttttaa atcggttaag
     1081 aagtttttcc aattatctaa attgggagta gtgcgagtgg ataattgtaa tttttccaac
     1141 accactttat ccaattcttc ttcttgcatt agtaatggaa cgtcgtaaat agttgatgca
     1201 tcgcgcgatt caattacggc attcatgctc acgttacaga atttggcaat tttgcgacga
     1261 atgtttaaat ccaactcatg ttctgttcta cacaccaaaa tatctggttg aacaccaaac
     1321 tcaagcaatt gtttaacaga gtgttgtgtt ggttttgttt ttaattcacc agcagcagcc
     1381 aaatacggca ccaatgttaa atggataaca atgcagttat tttcttcttc ccacatcaac
     1441 tggcgaacag cttccacata cggcaacgat tcaatgtcac caattgtacc accaatttct
     1501 gtaataacga tatcgaattc accgttttta cccaaaattt ggatgcgttc tttaatctca
     1561 tcggtgatgt gaggaataac ttgaaccgtt tttcctaaat aatcgccacg tctttctttc
     1621 tcaattaccg attggtagat gcgtcctgtt gttacgttgt ttgcttgcga tgtaggaaca
     1681 tttaaaaaac gttcgtaatg tcctaaatcc aagtcggttt cggcaccatc atcggttaca
     1741 aaacattcac catgctcata aggattaagt gttcctggat cgatgttaat atacggatcc
     1801 aatttctgaa tggtggttga gtaacctctt gcttgtaata atttagctaa agatgctgaa
     1861 ataatccctt ttcctaggga ggatgtaaca ccgcctgtta caaacacata ttttgttgaa
     1921 tttgacatgg aaaataaatg taactacggg gcgcaaagtt aatacaaact tttaagaatc
     1981 accctttttt attttattta attttccaca attggtggat ttgaaaatta atcttcttct
     2041 acgtagtctt cgggctcgtt gttttcttcg tcccaacgaa tcacgcgttg aacaccttct
     2101 acctcttcaa ttttattttt caataattcc aaatgcttcg tgtcgagtac cattacacgt
     2161 acttttcctt caaacaaacc accttctgtt tcaaaagaaa tggcgcgcat gttcacattt
     2221 tgttggttgg aaataatgcc tgttagcttg ttaacaattc ccatgcggtc aaatccataa
     2281 attttaatgg ttacttctct ttcctcaata tcttgatctt tccagcgagc attgatgcaa
     2341 cgataagcca tttttgacat taaattttcg gcatttggac aattggtgcg gtgaattttt
     2401 accccatcgt tgatggtgat gaatccaaaa actttgtcgc ctggaattgg tgaacaacat
     2461 ttagccaatg tgtagtggat atttttaaag ttttctccaa ttattaatgt atctgatttt
     2521 ttattgacag aagcttgaat gttatccttt ttacgtattc tctttgaagt atcttcactt
     2581 tttttgtgcg atagataacc gttatcattg tcaaactcgc gcaacttcgt tagatccatt
     2641 ttgccttttg caattctaaa atacaattct gttgcacttg gcaattgata ggctttttct
     2701 aagaacgtaa tgttttctga aacaaaaagg atgttgtgct gtttgaattt acgcatcaaa
     2761 atttctttac catcctcggc aatttgcttt ctttcctcgt ttagattggc ttttattttt
     2821 tgctttgcac gcgctgtaac caccatgctc aaccaatctt catttggttt ttgtttagaa
     2881 gaggtaatga tttctagctg atcgccactt tgcaattgat aggatagagg catcaatttg
     2941 ttgtttacct tggcgcctat acatgtgtcg cctatacgtg tgtgaatgtc gtatgcaaaa
     3001 tcgagaacag ttgcacccgt tggtaaaaca cgtaattctc cttttggtgt aaacacataa
     3061 atttcatcgt taaacaagtt taatttaaac tcgttgatga aatccatggc attcaaatct
     3121 ggattttcaa gtagagaacg aatttctgca atccatctgt ctaatttaga atcgtctttt
     3181 ttagattctt tgtatttata atgtgcagcc aaaccttttt cggcaatttc gtccattctt
     3241 ctggtgcgaa tttgcacttc tacccatttc ccttgtggcg acatcaccgt ggtgtgcaaa
     3301 gattcatatc cgttggcacg aggtgttgaa atccaatcac gcaaacgatc agggctgggt
     3361 tggtaaaaat cggtaacaat tgaatagact ttccaacaat cggctttttc taattccaaa
     3421 ggagaatcta aaataatgcg aatggcaaaa acatcataca cttcttcaaa tggaatgcct
     3481 ttggattgca ttttgttcca aatggaagaa atagatttgg tgcgtgcttt tatttcaaat
     3541 tggaatcctt cgttggtcat cgattcttta atcggtagta caaaacgacg gatgaagcgg
     3601 ttgcggacct cttgactgga ttttaaagca gtttcaattt gcttatacac ttctttttcg
     3661 gtgtatttaa aagataaatc ttctaattca gacttgatgg aatacaatcc taagcggtgt
     3721 gctaaaggag cgtataggaa ttttgtttca gacgcaattt ttagttgttt gtcttcgcgc
     3781 atgctgtcta gtgttcgcat gttgtgcaaa cgatcggcta atttgatgag tactacgcgc
     3841 aaatcttctg aaatagttaa aaccatttta cggaaattct ccgcctgaat ggatgcattt
     3901 tcatcaaaaa cttctggaat tttggttaat ccatcaataa tcagcctaac ttttgagcca
     3961 aaaacatctt caatatcttg taaagtgatg taggtgtctt ctacggtgtc gtgcaacaaa
     4021 gcacagataa tggcagttgg ccccaaaccc atttcctcca cacaaatacg tgccactgca
     4081 atgggatggt aaatataggg ttcacccgag gctctacgca tgtctttgtg cgcttcaaca
     4141 gccatgtcaa aagcttttcg aatcatttcg gtatcttttc tcgaacgatt ctttacatga
     4201 cgcattaatt gtttaaatgc acgtaaaatt tcaagacgtt ctttttctaa atcaatttgc
     4261 gaaatatcaa aacttgcttt tgccatatta tcaaaggtgt agagtttatt tatctggtta
     4321 gacaaatgta aaaaatagat ttttacttta gatagtggaa aaggataaaa aaagaatatt
     4381 tataaaaaag ccagagttga aaactaattc actctggctt taacttctta tttgttgttt
     4441 tactttgcag gcaaaacttt agagcttact tcgccaaatc ctatgcggat accgtctttt
     4501 tcagaataac cacgcataat tacagtgtcg ttatcttgaa tgaatttacg ctcagttcca
     4561 tcgttcattt ttaatggctt tgtacctttc catgctagtt ccaacataga accgtaggaa
     4621 tctggagttg gaccagaaat agttccagaa cccatcaaat caccacaacg cacattacaa
     4681 ccatttacag tgtgatgtgc caattgttgc gccatgttcc aatacatgta tttgaaattg
     4741 gatttacaaa ttaatgtttc tgtgctgttt tctggctgta aatacacctg caagttgatg
     4801 tcgtatgatt ttttaccggt gaattctaag tagctcaaca ccgcagggtc ttgctctgga
     4861 ccgtccactt taaaaggttg caaagcatcc aaggtaacaa tccaaggcga tagggaagaa
     4921 gcaaagtttt tagctaaaaa tggtcctaat ggaacgtatt cccacgtttg gatatcgcgt
     4981 gccgaccagt cgttgaacaa cgccattcca aaaatataat cttccgcctc attcgtggtg
     5041 atagaatctc ctaaaggttt gccatcatat gtgataaatg ctgtttctaa ttcaaaatcc
     5101 aatcgttggc acggtgcgta aatcggattt tc
//
LOCUS       DAOQVQ010000131         5114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_17991_length_5114_cov_3.559399, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000131 DAOQVQ010000000
VERSION     DAOQVQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17991_length_5114_cov_3.559399"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..494
                     /locus_tag="PKN22_05305"
     CDS             96..494
                     /locus_tag="PKN22_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS42156.1"
                     /translation="MGATKHEVYSKEINELANLFKALSNPARVKALLILAESEQKTIT
                     TEDFQNEIELSQSSISRHLSILSDSGLIATKIISKNKKSCLAYQLNYECIQILQQVIS
                     KITLLSGNRNTNSFYLKYKNIINAALLFKT"
     assembly_gap    531..540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(799..2409)
                     /gene="pckA"
                     /locus_tag="PKN22_05310"
     CDS             complement(799..2409)
                     /gene="pckA"
                     /locus_tag="PKN22_05310"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNS42157.1"
                     /translation="MDMFGKMGKNTDLKSTLGLDNLGNQFWNLTPSELVEDTILLGEG
                     ILTDTGAIAIETGEFTGRSPKDRFIVCDAKTENAVWWGDINIKFTPEKFDALFDRMKA
                     YMSGKDIYVRDNFVCADKNFRLNVRTINELPWGNHFANNMFIRPTDEELENFTPDWHI
                     VCMPNFMANPEIDGTRQHNFAILNFTKKMIIIGGTKYTGEIKKGVFSALNFILPHEKN
                     VLSMHCSANVGKDGDTAVFFGLSGTGKTTLSSDPNRKLIGDDEHAWADNSVFNIEGGC
                     YAKTIDLSREKEPQIYDAIKFGAILENIGFKDGSSTPDYTDNSITENTRVSYPLEHID
                     NIAVPSIGTAPKNIFFLTCDAFGVLPPISRLTNEQAMYHFMSGYTAKVAGTEVGITEP
                     STTFSACFGAAFLPLHPAKYAELLGKKLEGSDIKVWLVNTGWSGGAYGVGSRMKLSYT
                     RAMITAALEGKLNNTPFSKLPIFDLEFPTAVEGVPSEILNPKDTWADKAAYDETANNL
                     ANKFVKNFEKYADQASEAIKNAAPKVLA"
     gene            2715..3368
                     /locus_tag="PKN22_05315"
     CDS             2715..3368
                     /locus_tag="PKN22_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS42158.1"
                     /translation="MSQTIKIVIAEDISKIAEALKEKIELAVEFKVVDVVPNGEAMIK
                     RLENNHAVDVVLMDINMPEMDGVQATEIISNRWPNIKIIMCTVFDNEQNIFEAIMAGA
                     SGYLLKDETPAKVHKAIYEAIEGGAPMSSMIAKKALSLIKKGRPTEVVIEKDYELTKR
                     EMDVLEQIAKGLSYDQISENLFISYGTARKHVENIYKKMRVHNKIEAIEKAKKSGLI"
     gene            3441..>5114
                     /locus_tag="PKN22_05320"
     CDS             3441..>5114
                     /locus_tag="PKN22_05320"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42159.1"
                     /translation="MKVTLFTFFIFFSLSLLAQDATAVEQSILKLKGQQRIEKYLEVV
                     AEQTTRSPQLAIKLAQQALSENEIQKHSELHAKLLYHLGELYYLTGNFNKALSSLQQA
                     ETKLTQLNDKTVLPKVYISIGRIYKKAHNDCENTKKYYHKAEAIYTATNNLKELATVH
                     NYLGNITETCDNDLNKALSYYYQSEQYYITHKDTIGWSYSLDFISQVMAQQNNYKDAI
                     ARQTKALELRLAIKDSFAIAISYTNLGEIFLMQQQPHAAKENFKKAYAISGKSDYKDL
                     NIYLLGKLADISKEQNDYQSAYFYLGEQTALKDKLLDAEKHKQLAEMKTKYETEKKEA
                     ALSQEQIKNKNKTLWVILLIGAVVVLCIVIVLVVLSKKLERQKAELTILQNLDAERNR
                     IARDLHDNLGAELTLISSKLDIKSYKATNEAEKEELSAIRTISTNANFMLRETIWSIH
                     KQELTVDELYQKTKDYALRIFEDKETQVIVEVEQKETALSPSVALHLYRILQETINNA
                     SKYANCKTLTLHIAPHMVVVQDDGQGFDETIVSKGYGLQNIKQRVEELNG"
BASE COUNT         1773 a          864 c          873 g         1594 t
ORIGIN      
        1 tcgatatgta aatttctaat tattattttg agttttaaaa ttttattttt atcttcatac
       61 taataattaa ttattaacct tttacaatcc aacctatggg agcaactaaa catgaagttt
      121 actctaaaga aattaatgaa ctagcaaatc ttttcaaagc attatccaat ccagctagag
      181 ttaaagcatt actgattctt gcagaaagcg aacaaaaaac aattacaacc gaagattttc
      241 aaaatgaaat agagttatct caatcttcaa tttctcgtca tttaagtatt ttgtccgatt
      301 ctggtttaat tgctactaaa attatttcaa aaaataaaaa aagttgttta gcataccaat
      361 taaactatga atgtattcaa attctacagc aagttatatc taaaattact ctattgagtg
      421 gaaataggaa tacaaatagc ttttatttaa aatataaaaa tatcataaac gctgcattgt
      481 tgtttaagac ttagttattt atacctatta tgcatttaga aaacaaataa nnnnnnnnnn
      541 ttttaaattt attggtatgc atacagttaa cacaggtaaa cataaaaaaa tgagatttta
      601 gcacatattt ttctaacaaa atagggagaa gtgcttataa ctgagaaaaa gaatctagcc
      661 tttatgttga agaactaaaa agaaaacatt aaatatgttt aataatctgt atcggctatt
      721 taggactagt catacaaaca aaaataaaaa gaggccatcc ttttaggaca gcctcattgt
      781 ttatcttgca gttaaaaatt atgctaaaac tttaggtgcc gcatttttaa ttgcttcact
      841 tgcttggtcg gcatattttt caaagttttt cacgaattta tttgccaagt tattagctgt
      901 ttcatcgtaa gctgctttgt ctgcccacgt atcttttgga tttaaaattt cagaaggcac
      961 gccctcaacc gctgtaggga attccaaatc aaaaattggt aatttgctga atggagtgtt
     1021 gtttaatttt ccttccaatg ctgctgtaat catggctctt gtgtagctta atttcatacg
     1081 actaccaaca ccataagcac caccagacca accagtgttt accaaccaaa ctttgatgtc
     1141 agaaccttct aattttttac ccaacaattc tgcgtatttt gcagggtgta aaggtaaaaa
     1201 tgctgctcca aaacaagcag agaaagttgt gcttggttct gtgataccta cctctgttcc
     1261 agcaactttt gccgtgtagc cagacataaa gtgatacatt gcttgttcgt ttgttaaacg
     1321 tgaaattgga ggcaatacac caaaagcatc acatgttaag aagaaaatat tttttggagc
     1381 cgttccaata gaaggcaccg caatgttgtc aatgtgttct aatggataag aaacacgtgt
     1441 attttctgtg atagaattat cggtataatc tggagttgaa gaaccatctt taaagccaat
     1501 gttttctaag attgcaccaa atttaattgc atcgtagatt tgcggttctt tctcgcgtga
     1561 taaatcaatt gtttttgcat aacatccacc ttcaatgttg aaaacagaat tgtctgccca
     1621 tgcatgctca tcgtcaccaa ttaattttct gtttggatca gaagaaagtg ttgtttttcc
     1681 tgtacctgac aatccgaaga aaacggcagt atcaccatct ttaccaacgt ttgcagaaca
     1741 gtgcattgat aatacatttt tttcatgagg caaaatgaaa ttcaaagcag agaaaactcc
     1801 ttttttaatt tcacctgtat attttgttcc accaatgatg atcatttttt tagtgaagtt
     1861 caaaattgca aagttgtgtt ggcgtgtacc atcaatttca ggatttgcca taaaattcgg
     1921 catacacacg atgtgccaat ctggagtaaa gttttctaat tcttcatccg ttgggcgaat
     1981 aaacatgttg tttgcaaaat gatttcccca aggcaattca ttgatagtgc gcacatttaa
     2041 acggaagttt ttgtcagcac atacaaagtt atctctaaca taaatgtctt ttccagacat
     2101 ataagctttc attctgtcga acaaagcatc aaatttttct ggtgtaaatt taatgttgat
     2161 atctccccac catactgcgt tttccgtttt tgcatcgcaa acaatgaaac gatcttttgg
     2221 agaacgacca gtaaattcac ctgtttcaat ggcaattgcg ccagtgtctg ttaaaattcc
     2281 ttcacctaaa agaatagtgt cttctactaa ttctgatggt gttaaattcc aaaattgatt
     2341 tccaagattg tctaatccta gtgttgattt taaatcggtg tttttaccca ttttcccgaa
     2401 catgtccata atagtctaat ttttaagcta tgctattgat ttcgactgca aatttaagtc
     2461 atctattttg acaaaacgaa aaaataacta tttattttac gaacaggtta cgattttgtg
     2521 ttaacaactt ttcttttcta attaagaatc aaatggttaa taattattta aaaagcgtta
     2581 aataaattta aaataccttt tttaagcgaa ctgctttttg ctggattatt tgccattaaa
     2641 tgtgttaaat ccgtatttta acgtatttta ggataccaaa tattctatta tttttgaatc
     2701 aaaatttgat acagatgtca caaacgataa aaattgttat tgccgaagat atttctaaaa
     2761 ttgccgaagc gttaaaagaa aaaattgaat tagccgttga atttaaagtg gtggacgtag
     2821 ttcctaacgg tgaagcaatg atcaaacgtt tggaaaataa ccatgccgta gatgtggttt
     2881 tgatggacat caatatgcca gaaatggatg gcgtgcaagc aaccgaaatc attagcaatc
     2941 gctggcccaa cataaaaatc atcatgtgca cagtgtttga taacgagcaa aatatttttg
     3001 aagccattat ggctggcgca agcggttatt tattgaaaga tgaaacaccc gcaaaggtgc
     3061 acaaagcaat ttatgaagcc attgaaggtg gagcacctat gagttcaatg attgccaaaa
     3121 aagcattgtc gttgataaaa aaaggtcgac caacagaagt ggtgattgaa aaagattatg
     3181 aactcaccaa acgcgaaatg gatgtgctgg aacaaattgc taaaggtttg agctacgatc
     3241 aaatttcaga aaatttattc atcagctatg gaacggcacg caaacatgtg gaaaatatct
     3301 acaaaaaaat gcgggtgcac aacaaaattg aagccatcga aaaagctaaa aaaagtggtt
     3361 tgatttgaat tctacggatt ttctgtttgt aaatccctta ataacaatgt gatttcgtat
     3421 ttttttagta ttattactgc atgaaagtca ctctttttac tttttttatt ttcttttcac
     3481 tatcgctgct agcgcaagat gcaactgctg ttgaacaatc tattttaaaa ctaaaaggtc
     3541 aacaacgtat agaaaaatac ctagaagttg tggcagaaca aacaacacgt tctcctcaat
     3601 tggcaataaa attagcccaa caagcacttt ctgaaaatga aatccaaaaa cattctgagt
     3661 tacatgcaaa attgctctac catttgggag aattgtatta tttaactgga aattttaata
     3721 aagcgctatc atcgttgcaa caagccgaaa ccaaattgac tcaactcaac gataaaacag
     3781 ttttgccaaa ggtgtatatt tcaatcggga gaatctataa aaaagcccac aacgattgcg
     3841 agaatacaaa aaaatactac cacaaagccg aagcaattta cacagcaaca aataatctga
     3901 aagaattggc aactgtgcac aattacctcg gaaatattac cgaaacctgc gataatgatt
     3961 taaacaaggc gctttcttat tattatcaaa gtgagcaata ttacattaca cataaagaca
     4021 ccatcggatg gtcgtatagc cttgatttca ttagccaagt gatggcacaa caaaacaact
     4081 ataaagatgc tattgcgcgt caaacaaagg cgttggaatt gcgtttagcc atcaaagatt
     4141 cgtttgcaat tgccataagt tataccaatt taggtgagat ttttttgatg caacaacaac
     4201 cccatgcagc taaagaaaat tttaaaaaag cgtatgctat ttctggaaag tcggattaca
     4261 aggatttgaa tatttatttg ttgggtaaat tggccgatat cagcaaggaa caaaacgatt
     4321 atcaaagtgc ttatttttat ttgggcgagc aaactgcttt gaaagataaa ttgctagatg
     4381 ctgaaaaaca caagcaattg gcagaaatga aaaccaaata cgaaactgaa aagaaagaag
     4441 cagcactgag ccaagaacaa ataaaaaata aaaacaaaac cttgtgggtt atcctgttga
     4501 ttggtgctgt ggtggtgctt tgcatcgtga ttgttttggt agttttaagt aaaaaattgg
     4561 aacgtcaaaa agcagaatta acgattttgc aaaatttgga tgccgaacgc aaccgtattg
     4621 cacgcgactt gcacgataat ttaggtgcag aattgacctt gatttcttct aaattggata
     4681 ttaaatccta caaggcaacc aacgaagcag aaaaagaaga attgagcgcc attcgtacca
     4741 tttctaccaa tgctaatttt atgttgcgcg agactatttg gtccatccac aaacaagaat
     4801 tgaccgtaga tgagttgtac caaaaaacaa aggattatgc cctacgtatt tttgaggaca
     4861 aggaaaccca agtgattgtt gaggtggaac aaaaagaaac tgctttgtcg ccatcggttg
     4921 cattgcattt gtatcgcata ttgcaagaaa ccatcaacaa tgcttcaaaa tatgccaatt
     4981 gtaaaacgtt aacactacat attgcacctc acatggtggt ggttcaagat gatggacaag
     5041 gatttgatga aaccattgtt agcaaaggtt atggtttaca aaatattaaa caacgggtgg
     5101 aagaattgaa cggt
//
LOCUS       DAOQVQ010000132         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_18219_length_5074_cov_3.080295, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000132 DAOQVQ010000000
VERSION     DAOQVQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18219_length_5074_cov_3.080295"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..2723)
                     /locus_tag="PKN22_05325"
     CDS             complement(135..2723)
                     /locus_tag="PKN22_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42160.1"
                     /translation="MKNFILFLMLVCSTVVFGQAVTFSNDRIKFVKELQSSMAQYATE
                     DQKQFIKKELTVALLETKEISDKYFTNMVNTSNALLTKRFNLVPDVYNYIFSIYSLVK
                     NKQSEASYTAFQNTIDKLLESKNPKRFTDFADMSAGFFSTKKLAGKSNFEWFYMGGNY
                     EFKYDDKPYIQFTGGNLVCKALENGSSTKYVDSMVVYNTSGTYDPSQQRWEGKGGRVT
                     WEKVGLSKNETYAEVGNLKVSMKNTNMNIDSVRLKSPYLGETILGSLSDRAFKSNREE
                     DKIFPQFLSYNSNVKINNIKPNVNYEGAFSMKGAKFVGSGIKDIPAKIVILKNAKPFI
                     VAKAASIFISDKNINVPNCNTKIVLNTGDSITHPSNTFTYYYNEGTAEFSRPSSGLGQ
                     APFEDSYHKLFYYVPKIVLNEKENKIYFTYEKGTSQEQRVAKFESFNYYDGQLYDQLQ
                     GLSSVHPLVAISKYCYKYDKQEITEGECAAAIGGTIEQAKSMMLQLSNNGFISYDIEN
                     KKVFVNEKLDNFVKGKAGKKDYDNILFMCDFRPKELKGYSEEDIKSNAYLQQIRENYK
                     VQNEKRRVLENFGFFNMGTMDISFTGIDKITLSDKQNVHLFPSENNVVVKQNRDIEFS
                     GWINAGKAEINATSALYNYEANKINLIKTNETIFRVKPRKAEHGTAGLAMNSKINGVV
                     GDIVVDSPNNRSGNKEDATTAIYPVFSVKNATKVYYNSPELHKGAYDSTRFYYTIQPF
                     VLDSADNFNDFSWRLKGELVSAGIFPVIKEDLKVMPDYSFGFSTAAPKEGFDFYGTAK
                     YDNKVMLSGNGLQGAGTIKFVKSTSVSKSLLTFLPDSTVGVVSFVGVVPDTAGAVGDV
                     TSIVIV"
     gene            complement(2760..3602)
                     /gene="prmA"
                     /locus_tag="PKN22_05330"
     CDS             complement(2760..3602)
                     /gene="prmA"
                     /locus_tag="PKN22_05330"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNS42161.1"
                     /translation="MNYIELHIKLQPVQPFNEILVVQLADAGFESFVDTEDGILAYGQ
                     ITETSLERAINETFLGETRDDVQFTIEHQIIEHQNWNAVWESDFEPVYVEELVSILAP
                     FHEKSTAKGLIVEIQPQMSFGTGHHQTTWMMSKALFDIQPFPKKVLDMGTGTGVLAII
                     AEKLGATSILAIDIEDWSVENTVENAQRNNCSSIDSKCGDVDLIHGLKFDLILANINK
                     NVLKAHIPSYAQALDNKGILLLSGFFDSDVDELVTFAQQYGFEKEHVYQKDNWAAIKL
                     VRNK"
     gene            complement(3602..4345)
                     /gene="tpiA"
                     /locus_tag="PKN22_05335"
     CDS             complement(3602..4345)
                     /gene="tpiA"
                     /locus_tag="PKN22_05335"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNS42162.1"
                     /translation="MRKKIVAGNWKMNLSWVEANELFQNLAHARNEGVDVIIFPSTPY
                     LFPFASTADKNIAIGAQNAYPKDNGAYTGETSMAQLKSLGITHVLIGHSERREYFNES
                     NEFLKEKVDAALNHGLTPVFCCGEALEIREAGQENAFVTQQIEASLFHLSNEEMAKCV
                     LAYEPIWAIGTGKTATSQQAEEMHQTIRQFIAKKYGNALAESISILYGGSCNENNAHE
                     LFSCPNVDGGLVGGASLKADSFKKIIAAF"
     gene            complement(4352..>5074)
                     /locus_tag="PKN22_05340"
     CDS             complement(4352..>5074)
                     /locus_tag="PKN22_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42163.1"
                     /translation="NKKTKEEKLYDSSSPEYVASKIWENPEWEYIDMVQKTIKPTVLP
                     SITEQFNPFIAIADLTDIEKNMAVVKEFEASNFIQVVRVKNLSSNEIYNVPMEEYTIE
                     EYTPEYYQTLDTIQEPNPEVSDINIREYITTVDEIIVITTRDIEKANWENIERYKSIL
                     AGAKKHQIPMVLLSSSNREAINKFRKKYNFNIPVFTNDEIELKAIARSNPSMMIIKKG
                     IVVGKFPHRSTPTFDWMLKNKL"
BASE COUNT         1475 a          926 c          859 g         1814 t
ORIGIN      
        1 gatgcagatt taacaagcgg agctacaact atttatacta caagttatac tccaaattct
       61 tcattgtctg aaggaagtta tacattgtat gtacaggaaa aagattgggc aggaaactgg
      121 tcaacgagcg gaagctatac tattacaata gacgtaacgt cacctacagc gccagcagta
      181 tcaggtacaa cgccaacaaa tgatacaaca ccaactgttg aatcgggtag gaacgttagt
      241 aagcttttag aaaccgaagt tgatttcaca aatttaatgg ttccagcacc ttgcagtcca
      301 ttgccagaaa gcatcacttt gttatcgtat ttggccgtgc cgtaaaaatc aaatccttct
      361 tttggtgctg ctgtagaaaa accaaacgaa taatcgggca ttactttcaa atcttctttg
      421 ataactggga aaatacctgc agaaaccaat tcacctttta agcgccatga gaaatcgtta
      481 aaattgtcgg cgctgtccaa tacaaatggt tggatcgtat aataaaaacg tgtgctgtcg
      541 tatgctcctt tgtgcaattc tggagaattg taataaactt tggttgcgtt ttttactgaa
      601 aatacaggat aaatggctgt agttgcatct tctttatttc cagaacgatt gtttggagag
      661 tcaactacaa tgtctccaac aactccattg attttgctat tcattgcaag tcctgcagta
      721 ccatgttctg cttttcttgg ctttactcta aatattgttt cgttggtttt aattaagttg
      781 attttattgg cttcgtaatt gtaaagagca cttgttgcat tgatttcagc ttttccagca
      841 ttaatccaac cactaaactc aatatcacgg ttttgtttta ctacaacatt gttttctgat
      901 ggaaataaat gtacgttttg tttgtcggat aaggtaattt tatcaattcc ggtaaaacta
      961 atatccatag ttcccatatt gaaaaaccca aaattctcta aaacacgtct tttttcgttt
     1021 tgaactttgt agttttcgcg aatttgttga agataagcat tggattttat gtcttcttcg
     1081 gaatatcctt ttaattcttt tgggcgaaaa tcgcacataa acaagatgtt gtcgtagtct
     1141 tttttacctg cttttccttt aacaaaattg tctaattttt cgttgacaaa tacttttttg
     1201 ttttcaatgt cgtaactgat aaatccattg ttagataatt gcaacatcat ggattttgct
     1261 tgttcaattg ttccaccaat agctgctgca cattcacctt ctgtaatttc ttgtttgtcg
     1321 tatttgtagc aatatttact aatagcaacc aacggatgaa cagaagataa accttgcaat
     1381 tgatcgtata attgaccgtc gtaatagtta aaagattcaa atttagctac acgttgttct
     1441 tgacttgttc ctttttcgta agtgaaatag attttatttt ctttttcgtt taaaacaatt
     1501 tttggaacat aataaaatag tttatgataa gaatcctcaa aaggtgcttg tcctaaacca
     1561 gaagaagggc gcgaaaactc tgcagttcct tcgttgtaat agtaggtaaa tgtgttactt
     1621 ggatgtgtaa ttgaatcacc cgtatttaaa acaattttag tgttacaatt gggtacattt
     1681 atattcttat cagaaataaa aattgaagca gcttttgcta caataaatgg ttttgcattt
     1741 ttaagaataa caattttagc aggaatatcc ttaataccag aacctacaaa ttttgctcct
     1801 ttcatagaaa aagcaccttc atagttaaca tttggtttaa tgttgttgat ttttacattg
     1861 gaattataag ataaaaactg aggaaaaatt ttgtcttctt cacggttgga tttaaaagct
     1921 ctgtcggata aacttcctag aattgtttct cctaaataag gcgattttaa acgtacagaa
     1981 tcgatgttca tgttggtgtt cttcatcgat acttttaaat tgcctacttc tgcataagtt
     2041 tcgtttttag acaagcctac tttttcccaa gttacacgtc cgcctttgcc ttcccaacgt
     2101 tgttgcgagg ggtcgtaggt gccagaagtg ttgtaaacaa ccatactatc tacgtattta
     2161 gttgaacttc cattttctaa tgctttgcac actaagtttc cgccagtaaa ctggatgtag
     2221 ggtttgtcgt cgtacttaaa ttcataattt ccacccatat aaaaccattc aaaattggat
     2281 tttcctgcta attttttggt agaaaagaat cctgctgaca tgtccgcaaa atcggtgaat
     2341 cgtttgggat ttttactttc caataattta tcaatggtgt tttgaaaagc cgtgtaactt
     2401 gcttcacttt gtttgttttt caccaatgaa tagatagaaa aaatgtaatt gtacacatcc
     2461 ggcactaaat tgaatcgttt tgtcaacaaa gcgttggatg tattcaccat attggtaaaa
     2521 tatttatccg aaatttcttt tgtttccaac aaggcaactg tcagttcttt tttaatgaat
     2581 tgtttttgat cttcggttgc atattgcgcc attgaacttt gcaattcttt gacaaatttt
     2641 atgcgatcat tactaaaggt caccgcttgt ccaaaaacca cagttgagca aacaagcatt
     2701 aaaaaaagaa tgaagttttt catagttgtt taattaaagt tgttgttttt cgaattcaat
     2761 tatttattgc gcaccaactt aatagccgcc cagttgtctt tttgataaac gtgttctttt
     2821 tcaaaaccgt attgttgagc aaaagtcaca agttcatcta catccgagtc gaagaatccg
     2881 ctcaatagca aaataccttt attgtcaagt gcttgtgcat acgatggaat atgagctttc
     2941 aaaacgtttt tattgatgtt ggctaaaatt aaatcaaact ttaagccatg aattaaatca
     3001 acatcaccac attttgaatc aatagaagaa caattgttgc gttgtgcatt ttcaacagta
     3061 ttttccaccg accaatcttc aatatcgatg gctaaaatag aggttgcgcc tagtttttct
     3121 gcgataattg ccaaaacacc tgtgcctgtt cccatatcca atactttttt tggaaatggt
     3181 tgaatatcaa acaaagcttt agacatcatc cacgtggttt gatgatgccc tgttccgaat
     3241 gacatttgcg gttgaatttc tacaattaat ccttttgcag tcgatttttc gtgaaaaggt
     3301 gccaaaatac tcactaattc ttccacataa acaggttcaa aatcactttc ccaaacggca
     3361 ttccagtttt ggtgttcaat tatttggtgc tcaatagtaa attgaacatc gtcacgcgtt
     3421 tcacccaaaa atgtttcatt gatggcacgt tccaacgatg tttctgtaat ttgaccataa
     3481 gctaaaattc cgtcttccgt atccacaaaa ctttcaaatc cagcatcggc caattgcacc
     3541 actaaaattt cattgaatgg ttgaaccggt tgcaatttga tatgtaattc gatgtaattc
     3601 attaaaatgc agcaattatt tttttgaaag aatcagcttt tagagatgca cctcccacca
     3661 agccaccatc aacattgggg caagaaaata actcgtgtgc attgttttcg ttacagcttc
     3721 caccgtaaag aattgagata gattctgcta gtgcgttgcc gtatttttta gcaataaatt
     3781 gacgaatggt ttgatgcatt tcttctgctt gttggctggt agctgttttg cctgttccaa
     3841 ttgcccaaat aggttcgtag gcaagcacac atttagccat ttcttcgttt gacagatgaa
     3901 ataaactagc ttcgatttgc tgggtaacaa atgcattttc ttgtccagct tctctaattt
     3961 ccaatgcttc tccacaacaa aatacgggag tgagtccatg gtttaaagct gcatccactt
     4021 tttcttttaa aaattcgttg gattcgttaa aatattctct tcgttcactg tgtccaatta
     4081 acacatgcgt gatgcccaaa ctttttagtt gtgccatgga agtttcaccc gtgtatgcac
     4141 cgttgtcttt tggataggca ttttgcgccc cgatggcaat atttttatca gctgtggatg
     4201 caaaaggaaa taaataaggt gttgaaggaa aaattattac atcaactccc tcatttctag
     4261 cgtgggcaag gttttgaaac aattcatttg cttcaaccca cgataaattc attttccaat
     4321 ttcctgcaac tatttttttt ctcattgtat tttataattt gttttttaac atccaatcaa
     4381 atgttggggt agagcggtgt ggaaatttcc ctacaactat ccctttttta ataatcatca
     4441 tggatgggtt acttcttgca attgctttta attcaatttc gtcattggta aaaactggaa
     4501 tgttgaaatt gtattttttt ctaaatttat tgatagcttc tctgttagaa ctactcaaaa
     4561 gcaccattgg aatttgatgt tttttagcgc ctgccaagat agatttgtag cgttcaatat
     4621 tctcccaatt agctttttca atgtctctgg ttgttatgac tataatttca tctacagttg
     4681 ttatgtattc acgaatgttg atatcgctca cctcagggtt gggttcttga atggtatcca
     4741 acgtttggta atattctggc gtatattctt cgatggtgta ttcctccatc ggaacattgt
     4801 aaatttcgtt ggagctcaag ttttttacac gcaccacttg aatgaaatta gacgcctcaa
     4861 attctttaac tactgccata tttttttcaa tatccgttaa atcagcaatt gcaataaaag
     4921 ggttgaattg ctcggtaatt gacggtaaaa cagttggttt tatggttttt tgcaccatgt
     4981 caatgtactc ccactctgga ttttcccaaa ttttagaagc aacgtattca ggagaagaag
     5041 aatcatacaa tttttcttct tttgtttttt tgtt
//
LOCUS       DAOQVQ010000133         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_18621_length_4996_cov_3.590569, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000133 DAOQVQ010000000
VERSION     DAOQVQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18621_length_4996_cov_3.590569"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKN22_05345"
     CDS             complement(<1..184)
                     /locus_tag="PKN22_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42164.1"
                     /translation="MKFNTRQNEFYFISDGDSIITLKSGKFCWKKVGDVKIALFYDFK
                     KRVIIPDPNMHLADFVN"
     gene            complement(195..836)
                     /locus_tag="PKN22_05350"
     CDS             complement(195..836)
                     /locus_tag="PKN22_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNS42165.1"
                     /translation="MFHVISDIHGNLSSLEKSLSKCPTQCEQIFLLGDIGFGFEEFDV
                     KGYYELITFYNRKGKMITAIQGNHDNAEAMSSVEVTTLDGPEVYVYNGKFFLLIPGAL
                     SYDKKLRVEGVSWWRNEEMNHAHCNETFDLIVNYNFDYILSHDAPLSKYLDFFSQTLP
                     SATNTMLNGILTKLSEKNKKSTWIHGHLHQSYFEQVNNVNLIGVAEDTYITIF"
     gene            complement(836..1714)
                     /locus_tag="PKN22_05355"
     CDS             complement(836..1714)
                     /locus_tag="PKN22_05355"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNS42166.1"
                     /translation="MNQFKKYQHLERLYKKEVSGIDQGTCYIFSKLDGTNSQIWLENG
                     ELKAGSRNNILTLEKDNYGFYQYVLNNKDLYLPFFTILPNAILYGEFLKIHNIKTYRK
                     DALDKFYVFDVMLNDHYIEYDTYQLTLEKLGILYVPLLAKVNDYKGDFSEFLPQADFL
                     LESGHGEGIVIKNYDFVNKYGETKWAKLINENFSLEKGKPRPVRNEADNTLEQKIVDE
                     LVTEHFVKKEYTKLIAQLGEGVKGIQGQLLRVVNKTLIDEEGSFIVEQYGENVDFKLI
                     TKFTTLKVKQVLPELF"
     gene            complement(1774..2055)
                     /locus_tag="PKN22_05360"
     CDS             complement(1774..2055)
                     /locus_tag="PKN22_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42167.1"
                     /translation="MENISGFQLTFDKMIAAIYNAYGDELNEFSSFMLILNGDGSIEI
                     MQNKVEEFEDRKEEYAFLIDLETFTSTVEEKYQESFGEPFNIDFTDLYY"
     gene            complement(2059..2499)
                     /locus_tag="PKN22_05365"
     CDS             complement(2059..2499)
                     /locus_tag="PKN22_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42168.1"
                     /translation="MNRQQLVEYVAIETKYEKLQDELYTIIDRIILKLNGKERNLYYG
                     EYNGISGITFLFENNKLLVDFTGYYRGDTDNYKIELPIYLIENYSEDTLNIFLQEYLI
                     EKERKREEQQKIKKQLEEAKLLEVQKEQYRSLYKKFNGVLPESL"
     gene            complement(2605..3750)
                     /locus_tag="PKN22_05370"
     CDS             complement(2605..3750)
                     /locus_tag="PKN22_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42169.1"
                     /translation="MKNIRWTKERVIEQFNRLEDKDNFLLETPYEIKCRTSKIRIQCK
                     RCNYVWYPNINNFFQNHARCNICNKNIWTHERFWSEFNKLPDKNLYTVEELPKKLSAR
                     TKINLICNNKHIFSPTIQNFFYHGTRCMICSRGEPWSKFRFLKEIEALDDKDNFIFSD
                     YEDINKVTSTFSVYCKKCTHIWKATPTNFFQKGTRCKKCVMNAWSNARVEKEFNNTKD
                     SKKFSLIFTEKIIHGQSCFKVKCKYGHIWKTTPSRFFTAKKRCPLCNISRGERKICEI
                     LEFNNIIFENQKRFKNCKLKYTLPFDFYLLDYNVLIEFQGKQHFEVANFNSNLEINKQ
                     QFIDLQHRDSIKRNWVISNGYTLLEIRYDEMNQIESKLMDLLNVYYI"
     gene            complement(3949..>4996)
                     /locus_tag="PKN22_05375"
     CDS             complement(3949..>4996)
                     /locus_tag="PKN22_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2828 family protein"
                     /protein_id="HNS42170.1"
                     /translation="MSLAAKYAPDQNYKNKDFFNAYFNVASKIFDYNRKEYRQLLSAM
                     RKALNIVEVKMSANRWDRINFENVPSVAHKNYRKAFGKHQPDRYIQYLNAVKSGEKKI
                     NAKVLTPVDVVKQYIDEAGNFKVYGIDETLEELWKNLPGKVESNVLPIADTSSSMSGI
                     PMLVSIALSIFCAQRSEGAFKNEVISFNDNPMFHSLEKCKTLRDCITELKKMKWGGST
                     SITNTFFKLLETAKKNKIKQEEMPKFILLISDMEWNFSSGRNETPLQEIEKEFNDSGY
                     LLPTLIMWDVRHGEQKSYPSQDKQGIFQISGYSNSVLNYVWAVMNGTSLDVVVEILNS
                     HRYSFVDDLLYFKS"
BASE COUNT         1626 a          862 c          625 g         1883 t
ORIGIN      
        1 gattaacaaa atctgccaaa tgcatattag gatctggtat tatcactctt tttttgaaat
       61 cataaaaaag agcaatttta acatctccaa cttttttcca acagaattta cctgatttaa
      121 gtgtgataat actatcgcca tcgctaatga aataaaactc attttgtctc gtgttgaatt
      181 tcattttccc ctccttaaaa aattgtaatg taagtatctt ccgctacccc aattaaattt
      241 acattattaa cttgttcaaa ataagattgg tgtagatgcc catgaatcca agttgatttc
      301 ttattttttt cacttaactt tgttaaaatt ccgttcaaca ttgtattggt tgcacttggc
      361 aatgtttgac taaagaaatc aagatacttc gataatggtg catcatgact taaaatgtaa
      421 tcaaaattat aattaacaat taagtcaaat gtttcattac aatgagcatg attcatttcc
      481 tcattacgcc accatgatac accttcaact ctgagttttt tatcataaga taatgcacca
      541 ggaataagaa gaaaaaactt accattataa acataaactt caggtccatc taaagttgta
      601 acttcaacac tactcattgc ttctgcgtta tcatgattgc cctgaatagc tgtaatcatc
      661 ttgccttttc tattgtaaaa ggttataagc tcataatagc cttttacatc aaattcttca
      721 aatccaaacc ctatatcacc taatagaaaa atttgttcgc attgggttgg acatttactt
      781 aaacttttct ctaaagagct taagttgccg tgaatatcgc taattacatg aaacattaaa
      841 acaactccgg taatacttgc ttgaccttca gtgttgtaaa cttcgtaata agtttaaaat
      901 caacattctc accatactgc tctacaataa aacttccttc ttcgtcgatc aaagtcttat
      961 ttacaacacg taatagctga ccttgaattc ctttaacacc ttcacctaat tgtgcaataa
     1021 gttttgtgta ttctttctta acaaagtgtt ccgtcacaag ctcatcaaca atcttttgtt
     1081 ctaacgtatt gtcggcttca tttcttacag gtcgtggctt acctttttct aaagaaaaat
     1141 tttcattaat gagctttgcc cattttgttt caccatactt gttaacaaaa tcataatttt
     1201 tgatgacaat accttctcca tgaccacttt ctaacaaaaa gtctgcttgt ggtaaaaatt
     1261 cactaaaatc gcctttatag tcgtttactt tagcaagtag aggaacatat agaattccta
     1321 gtttttctaa agttaactga taagtgtcat attcaatata atgatcattt aacattacat
     1381 caaaaacata gaatttatct aaagcatctt ttctatatgt cttgatattg tgaattttta
     1441 aaaattctcc ataaagtata gcgtttggta aaattgtgaa aaatggcaaa tataaatctt
     1501 tgttatttaa aacatattga taaaatccgt aattatcttt ttctaacgta agaatattat
     1561 ttcttgaacc agcctttaac tcgccatttt ctagccaaat ctggctattt gttccatcaa
     1621 gttttgaaaa aatgtagcaa gttccttgat ctattccgct tacttctttt ttatatagcc
     1681 gttctagatg ttgatatttt ttgaactgat tcataaattt cctttctcca tctcaattga
     1741 tactaatata tcatagattt taaatttgtc aaatcaataa tataaatcag taaaatcaat
     1801 attaaatggt tcaccaaacg attcttgata cttttcttca actgttgatg tgaaagtttc
     1861 aaggtcaatc aagaatgcat attcttcttt tctatcttca aattcttcaa ctttattctg
     1921 cataatctca attgagccat caccatttaa aattagcata aatgaagaga attcgttcag
     1981 ttcatcacca taggcattat aaatggcagc aatcatttta tcaaatgtaa gttgaaaacc
     2041 tgaaatattt tccataattt ataaactttc tggtaaaaca ccattgaatt tcttgtatag
     2101 actgcgatat tgttcttttt gtacttccaa aagcttagct tcttctaatt gtttttttat
     2161 cttttgctgt tcttcacgct tacgttcttt ttcaataaga tactcctgca aaaaaatatt
     2221 aagggtatct tctgaataat tttcaattag ataaattggt agctcaatct tataattatc
     2281 tgtatcgcct ctataataac ctgtgaaatc aactaataat ttattgtttt caaacaagaa
     2341 tgtaatgccg ctaataccat tgtattcgcc ataatataaa ttacgttctt tgccattcag
     2401 ctttaaaata attctatcaa ttattgtata taattcatct tgcaattttt catatttcgt
     2461 ttcaattgct acgtattcga ctaattgttg tctattcact gttgttttcc ttttattaat
     2521 tagcttaaca ttgttgttga tgaatttatt atatcacagt ttaaaacgtt taacttgaca
     2581 gaattcatac gattgccctg cggtttagat gtaataaaca ttcaataaat ccattaattt
     2641 tgattctatt tgattcattt catcgtaacg tatttctagt aaagtgtagc cgttggagat
     2701 tacccagttt cgtttgattg agtcacgatg ttgaagatca ataaactgtt gtttgtttat
     2761 ttctaaatta ctattaaaat ttgcgacttc aaaatgttgt ttcccttgaa attcaatcaa
     2821 tacattataa tctaacaaat aaaaatcaaa tggtaaagta tattttaatt tgcaattttt
     2881 aaatcttttt tggttttcaa atattatatt attaaattct aaaatttcac atatttttcg
     2941 ctcgccacga gagatattgc acaatgggca acgctttttg gcagtgaaaa aacgacttgg
     3001 agttgttttc caaatatgcc catatttaca ttttacctta aaacaacttt gaccatgtat
     3061 aattttctct gtaaatatta aagaaaattt cttagaatct tttgtattat taaattcttt
     3121 ttcaactcta gcgtttgacc aggcattcat tacacatttt ttacatctgg ttcctttttg
     3181 aaaaaaattt gttggagtag ctttccaaat atgcgtgcat tttttacaat agacagaaaa
     3241 tgtagatgtt actttattga tatcttcata atcactgaaa ataaaattat ctttatcgtc
     3301 aagtgcttct atttctttta aaaatctaaa tttactccac ggttcgcctc tggagcaaat
     3361 catgcaacgc gtcccatgat agaaaaagtt ttgaatcgtt ggactaaata tgtgtttatt
     3421 gttacaaatt aaatttattt ttgttctagc tgataatttt ttcggcaatt cttctacagt
     3481 atatagattc ttatcgggca atttattaaa ttcagaccag aatctttcgt gagtccaaat
     3541 atttttatta caaatattac atcgagcatg attctgaaaa aaattattta tgtttggata
     3601 ccaaacataa ttacatcttt tgcattgaat tcttatcttg gaagtacgac attttatttc
     3661 atatggtgtt tctaacaaaa aattatcttt atcttctaat ctattaaatt gttcaataac
     3721 tctttctttt gtccatctta tgtttttcat aaaaaaattc ctttgaaaat atttagcctg
     3781 gacaattgcc aaagatattc ccaaaggaaa caagtagaat tatattaagc gcataaagcg
     3841 ataataccat attgatttaa ttctacttgt gaacaactgc ccaagtcgtt tttaagtaga
     3901 attaacatat cacatcttaa ttgaaatgtc aagagtgata taatactctt atgatttaaa
     3961 atacaataaa tcatcaacaa aggagtagcg atggctattt aatatttcca cgacgacatc
     4021 tagacttgtt ccgttcataa cagcccatac gtaattcaaa actgaatttg aataacctga
     4081 aatctgaaag atgccttgct tatcctgact aggataactt ttttgttcac catgtctaac
     4141 atcccacatg atcagcgtcg gcaataaata accggagtca ttaaactctt tttcaatttc
     4201 ttgcaaagga gtttcgtttc taccagatga aaaattccac tccatgtcac taattaatag
     4261 aataaatttt ggcatttctt cttgtttaat tttattcttt ttagcagttt ctaaaagttt
     4321 aaaaaatgta ttcgttattg aggtacttcc accccatttc atttttttca attctgtaat
     4381 acaatctcgc aaagttttac atttttcaag tgaatggaac attggattat cgttaaagct
     4441 gattacttca ttcttaaatg caccttcaga acgttgagcg caaaagatcg aaagtgcaat
     4501 actaacaagc attggaattc ctgacattga agaagaagta tccgcaattg gtaaaacatt
     4561 agattcaact ttacctggga gatttttcca caattcttcc aaagtttcat caatgccata
     4621 aaccttaaaa ttacctgctt catcaatata ttgctttaca acatctacag gtgttaatac
     4681 cttcgcattg attttctttt caccagactt gacagcatta agatattgga tatatcgatc
     4741 aggttgatgc tttccaaaag ccttccgata gttcttatga gcaacacttg gaacattttc
     4801 aaaattaatt ctgtcccaac gattagcgga cattttaact tcaacaatat ttaaagcctt
     4861 acgcattgct gaaagtaatt gacggtattc tttccgatta taatcaaaaa ttttacttgc
     4921 aacattaaaa taagcgttga aaaaatcctt gtttttataa ttttgatctg gtgcgtattt
     4981 cgctgcaagt gacata
//
LOCUS       DAOQVQ010000134         4982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_18693_length_4982_cov_3.871727, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000134 DAOQVQ010000000
VERSION     DAOQVQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18693_length_4982_cov_3.871727"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKN22_05380"
     CDS             <1..344
                     /locus_tag="PKN22_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HNS42171.1"
                     /translation="LDMPFISLACKLDVNGTTLKLEKEYLGGIQEIETSLPAVVSCAK
                     GMAEQRIPNMRGIMAARTKPLTVVPAAPVEELVSFVGYTMPAAKSAVKMIAPDNMDEL
                     VSLLHNEAKVI"
     gene            369..1307
                     /locus_tag="PKN22_05385"
     CDS             369..1307
                     /locus_tag="PKN22_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNS42172.1"
                     /translation="MSVLVYINAGHGIGKNAFEAVTYGKKLGDVTVVTNGNVDDAALA
                     KLGEYGASKVLVDKSLTAADDQQLTRLVENAANQVGANTIVFVHDHVAKNVAPRLAVR
                     MKAGLVSGAIALPEGDGSVKVNVFSGKAFGSVKVNTAKKIISLLPNSIQPENGGGSAT
                     VETFAANAGDAKVKVLATKQPEGEIPLPEAELVVSAGRGLKGPENWGIVEDLAKALGA
                     ATACSRPVSDADWRPHHEHVGQTGIAIRPNLYIAVGISGAIQHLAGVNGSKTIVVINT
                     DAEAPFFKAADYGVVGDAFEVLPRLTEAVKKFKASH"
     gene            1389..1991
                     /locus_tag="PKN22_05390"
     CDS             1389..1991
                     /locus_tag="PKN22_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNS42173.1"
                     /translation="MNKIKLDIVGLSYSQTQSGAYALVLAESGGKRRLPIIIGGFEAQ
                     AIAIELEKMTPTRPLTHDLFKAFALTFEVQVKEIFIYDLVEGIFFSKIICEQNGDFYE
                     IDARTSDAIAIGVRFSAPIYTLESILSSAGILLDDDLLEEGEKAIESEGAMNETMPQS
                     NPFQKMSLDDLKRKLEEAIHNEEYELASKIRDEINNRSTN"
     gene            2006..3265
                     /locus_tag="PKN22_05395"
     CDS             2006..3265
                     /locus_tag="PKN22_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013447225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HNS42174.1"
                     /translation="MLKDLKLKLTVMSFLQFFVWGAWLITIGIYCSQTLKWSFSEFGA
                     IFSTMGISSLFMPTLIGFIADKWINSEKLYALLHLGNASAILYLTTVNDSTTFFWVVL
                     FAMICYMPTLALSNSIAYQILKSRNLDVVKVFPSIRVWGTVGFIAAMWVTNLTGNKAT
                     EGQFYISAIAGVLLALFSLFVLPKCPPQYTGKSVEKSNIIKDAFYLFADYKMALFFFF
                     SMLLGGALQLTNMYGDSFLSSFEAIPQYADSLVVRYSTLIMSISQISETLFILAIPFF
                     LKRYGIKNVMLMSMFAWVLRFGLFAYGNPADGLWMIILSCIVYGMAFDFFNVSGSLYV
                     ETTANPKIRASAQGMFMMMTNGVGAILGSSISGVVIDKYFVLADGSLDWTGVWLSFAL
                     YALIVAVLFAVLFKHKHTPLTDEQISH"
     gene            3355..>4982
                     /locus_tag="PKN22_05400"
     CDS             3355..>4982
                     /locus_tag="PKN22_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42175.1"
                     /translation="MTKLRITFLNLFSKAIVGIFAFGLSFNSFGQIIRDTISAVGLQT
                     WTVPANVTNITIDVWGAGGGGGGAESHILNYAKPRAAGGGGGAFSSGSFAVTSSDVLS
                     ITVGEGGIGGTSNQDGAAGGATIVELAGTTIVSADGGQGGAFRDCEGNVGTATGLGGS
                     AGTLFLYAGGNGAPSNTTEFGAGGGGAAGTTQAGTNGTGSTGGLGGLVGGGNGGDPTT
                     LQDEDGKPGALPGGGGSGARRTDLAGSTKGGNGANGMVIISYCVLPTAPTIATGSLTP
                     CEQSNETYTLTADANVDYYVWTLPNGWSGLSTSNSIDVVVGQPGTITVEAFNVCGQST
                     TETFTINVTELPAQPSAISGNAIACEGTQQTYAVTNDVTVDSYTWTLPGDWTGASTTN
                     SIVATIGTQAGMISVVANNICGSSAASILNATISNLPAQPSAINGNTTFCQGDNAAFN
                     VASNPEVTNYVWTFPADWTGTSTTNIINLTVGATSGDVTVAAVNSCGSSTAQTLTITV
                     NPVDATVTNNNNVLTANQAGAVYLWIDCATGAPVA"
BASE COUNT         1478 a          809 c         1115 g         1580 t
ORIGIN      
        1 cgttggatat gccatttatt tcattggctt gcaaattgga tgttaacgga acaacattga
       61 agttggagaa agaatatttg ggtggaattc aagaaattga aacatcactt ccagcagttg
      121 tttcttgtgc aaaaggaatg gcagagcaac gtattccaaa catgcgtgga attatggctg
      181 caagaacaaa acctttaacg gtggtaccag ctgctccagt tgaagagttg gttagctttg
      241 ttggttacac aatgccagca gctaaatctg cagtgaaaat gattgctcca gacaacatgg
      301 atgaattagt ttctttgtta cacaacgaag ctaaagttat ctaattatta attttataca
      361 aagtagatat gtcagtttta gtatatatta atgcaggaca cggaatcggg aaaaatgctt
      421 ttgaagccgt gacttatgga aagaaattag gagatgttac cgttgttaca aatggtaatg
      481 ttgatgatgc agcacttgct aaattgggtg agtatggtgc atcaaaagta ttggttgata
      541 aatcgttgac agcagcagat gaccaacaat taacacgctt ggttgaaaac gctgctaatc
      601 aagttggtgc caacacaatt gtttttgtac acgaccacgt agcgaaaaac gtagcgcctc
      661 gtttggctgt tcgtatgaaa gctggattgg tttcaggagc aattgcattg ccagaaggtg
      721 atggttctgt gaaagttaac gtattttcag gaaaagcttt tggaagtgtg aaagtaaaca
      781 cagctaaaaa aattatttca ttgttgccta actctatcca acctgaaaat ggtggtggaa
      841 gtgcaactgt tgaaactttt gctgccaacg caggagatgc aaaagtgaaa gttttggcta
      901 ctaaacaacc agaaggtgaa attccattac cagaagctga attggtagtt tctgctggtc
      961 gtggtttgaa aggaccagaa aactggggaa ttgttgaaga tttagcaaaa gctttgggtg
     1021 ctgcaactgc atgttcacgt ccagtttctg atgctgattg gagacctcac cacgagcacg
     1081 ttggacaaac aggtattgct attcgtccaa acttgtacat tgcagttgga atttctggtg
     1141 caattcaaca cttggcggga gttaatggtt ctaaaacaat tgtggtaatt aacacagatg
     1201 ctgaagcacc attctttaaa gctgctgatt acggtgttgt tggcgatgca tttgaagtat
     1261 tacctcgttt aacagaagct gtgaagaagt ttaaagcttc tcactaatta ataaaattca
     1321 ataaaaatag ggaagtgtag tttatacttc cctatttttt gttattttag aaaactcttt
     1381 caatcagaat gaataaaata aaacttgata tagtaggact ttcatacagt caaacgcaat
     1441 ctggcgctta tgcgctggtg ttggcagaat cgggaggtaa acgtagatta ccgattatta
     1501 ttggtggatt tgaagcacaa gcaattgcca tagaattgga aaaaatgacg cctacacgac
     1561 cgttaacaca cgatttgttt aaagcgtttg cacttacatt tgaggttcaa gtgaaagaaa
     1621 tttttattta tgacttggtg gaaggaattt tcttttctaa aattatttgc gagcaaaacg
     1681 gtgattttta tgaaattgac gcacgcacgt cggatgccat tgccataggt gtacgtttta
     1741 gtgctccaat ttatacgctg gaaagcattt tgtcgagtgc cggcatattg ttggacgatg
     1801 acttattaga agaaggagaa aaagccattg aaagtgaagg cgcaatgaat gaaacgatgc
     1861 ctcaaagcaa tccttttcaa aaaatgtcgt tagacgattt aaagagaaaa ttagaagaag
     1921 ctattcacaa tgaagaatat gaattggctt caaaaattag agatgaaatt aacaatcgct
     1981 ctaccaatta attactactt atcacatgct taaagattta aaactaaaac ttaccgttat
     2041 gagcttctta caattttttg tatggggcgc atggttaatt actattggta tttattgtag
     2101 tcaaacccta aaatggtcgt tttctgaatt tggtgcaatt ttctcaacaa tgggaatttc
     2161 atcgttgttt atgccaactt taattggttt tattgcagat aaatggatta actcagagaa
     2221 attatatgcg ttgttacatt taggcaatgc ttcggcaatt ttatatttaa caacagtcaa
     2281 cgattccacc acttttttct gggtggtgtt gtttgctatg atttgttaca tgcctacatt
     2341 ggcgttgtct aactctattg cctatcaaat attaaaatca cgaaatttag atgttgtaaa
     2401 agtatttcct tccattcgtg tttggggaac tgtaggcttt attgcagcga tgtgggttac
     2461 gaatttaact ggaaataaag caacagaggg acagttttat atttcggcaa ttgctggagt
     2521 tttattagcc ttgttttctt tgtttgtatt acctaagtgt ccgccacaat acaccggaaa
     2581 atcggttgaa aagtcaaata ttatcaaaga tgcgttttat ttatttgccg attacaaaat
     2641 ggcgcttttc ttcttctttt ctatgctgtt gggtggggct ttgcagttga ccaatatgta
     2701 tggagatagt tttttatcca gttttgaagc aataccgcaa tatgcagatt cattggtggt
     2761 gcgctattct accctcatta tgtcaatttc acaaatttcc gaaacattat ttattcttgc
     2821 cattcctttt ttcttaaaac gctatggaat taagaacgta atgctgatga gtatgtttgc
     2881 ttgggtattg cgttttgggt tgtttgctta tggaaatcca gcagatggct tgtggatgat
     2941 tatcctttca tgtattgtgt atggaatggc ttttgatttt ttcaatgtat cgggttctct
     3001 ttacgtagaa actacagcaa atccaaaaat tagagccagt gcacaaggaa tgtttatgat
     3061 gatgaccaat ggcgtagggg caattttggg aagttctatc agtggtgtgg ttatcgataa
     3121 atactttgta cttgccgatg gttcgttgga ttggacaggt gtttggttat cgtttgcttt
     3181 gtatgccttg attgttgcag ttttatttgc agtacttttt aaacacaaac acacgccttt
     3241 aacagatgaa caaatctctc attagttcac attaattcgt ttatttgtta aaaattttgt
     3301 cagatttaat tttaggtgta gatttgcgcc cattaactaa ttaaatcatt tactatgaca
     3361 aagttaagaa tcactttctt aaatttattt tcaaaagcga tcgtaggaat ttttgcattt
     3421 ggattgagtt tcaattcatt tggtcaaatt attagagata cgatttcagc agttggttta
     3481 caaacatgga ctgtcccagc caacgttacc aacatcacta tagatgtttg gggagctgga
     3541 ggtggcggcg gtggtgccga aagtcacatt ttaaattatg ctaaacctcg cgctgctgga
     3601 ggtggcggtg gagctttttc tagtggctca tttgctgtaa cgtcaagcga tgttttatca
     3661 ataactgttg gtgagggagg tattggtgga acttctaatc aagatggagc agctggaggt
     3721 gctacgatag tagaattagc tggaacaact attgtgtctg cggatggtgg tcaaggtggt
     3781 gctttccgtg attgcgaagg aaacgttggt actgctacag gtttaggagg tagtgctggt
     3841 acattgtttt tatatgctgg aggtaacgga gctccatcaa atacgactga atttggagcg
     3901 ggtggcggtg gtgccgctgg tacaacacaa gcaggaacta atggaacagg ttcaacaggt
     3961 ggacttggag gccttgtagg tggtggaaat ggtggtgatc caacaactct gcaagatgaa
     4021 gatggaaagc caggtgcatt gcctggaggt ggtggtagtg gtgccagaag aacagatttg
     4081 gcaggttcta caaaaggtgg caatggagca aatggaatgg ttattatttc ttattgcgtt
     4141 ttgccaacag caccaacaat tgcaactggt tctttaactc cttgtgagca atcaaacgaa
     4201 acatacacat taacagcaga tgccaatgta gattattatg tatggacatt gcctaatggt
     4261 tggagtggtt taagtacatc taactctatt gacgtagttg ttgggcaacc tggaacgatt
     4321 acagttgaag cattcaacgt gtgtggtcag tctactacag aaacgtttac aattaacgta
     4381 actgaattgc ctgctcaacc gtcagcaatt tcgggtaatg caatagcatg tgaaggtaca
     4441 caacaaacgt atgctgtaac aaacgatgta acagttgatt catacacatg gactttgcca
     4501 ggagattgga caggcgcaag cacaaccaac agcattgttg caacaattgg tacacaagca
     4561 ggaatgattt ctgtggttgc caacaacatt tgtggttctt ctgcggctag tattttgaat
     4621 gcaacaattt caaatttacc agctcaacct tctgcaatta atggtaacac aactttttgt
     4681 caaggagata atgctgcatt taacgtagca tcaaatccag aagttaccaa ttatgtttgg
     4741 actttccctg ctgattggac aggaacaagc acaacaaaca ttattaattt aacagttggc
     4801 gctacatctg gtgatgtaac agttgctgcc gtaaatagtt gtggatcatc tacagcacaa
     4861 acattaacaa ttacagtaaa tccagttgat gcaacagtaa ctaacaataa caatgtgttg
     4921 actgcaaatc aagctggtgc tgtttatttg tggattgact gtgcaacagg tgctccagtt
     4981 gc
//
LOCUS       DAOQVQ010000135         4943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_18894_length_4943_cov_3.507570, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000135 DAOQVQ010000000
VERSION     DAOQVQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18894_length_4943_cov_3.507570"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKN22_05405"
     CDS             <1..581
                     /locus_tag="PKN22_05405"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HNS42176.1"
                     /translation="QLIVGEMNFAFEHRVAPQSSLEVSFGPTVSEIGINKIYFDNVLN
                     NNNAIYGLNSRTQSGLGFFLGLSYRYYPISGASTAPRGLYLGPEIKYRMYNTYYIDNL
                     GVLENRTGSANQLLFRFHTGYQFVIGKKFAIDVFTALGLGYTVINAYYDIQLYNSQTG
                     GFTTSWYSKTRNRVFVSGSVGVKFGLGGNRKE"
     gene            681..1058
                     /gene="gcvH"
                     /locus_tag="PKN22_05410"
     CDS             681..1058
                     /gene="gcvH"
                     /locus_tag="PKN22_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNS42177.1"
                     /translation="MSIPANLKYTKDHEWIRIEGETAVVGITDFAQSELGDIVYVEVE
                     TEGETIEQNEVFGSVEAVKTVSDLFLPVTAEILEFNTNLEANPELVNSDPYGEGWIVK
                     IKIADASQIDSLLDAAAYEAVIG"
     gene            complement(1195..1455)
                     /gene="rpmA"
                     /locus_tag="PKN22_05415"
     CDS             complement(1195..1455)
                     /gene="rpmA"
                     /locus_tag="PKN22_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNS42178.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGVKIFGGQAAIAGNIIVRQRGTQ
                     HHAGENVGIGKDHTLFALTDGIVEFRKKKDNRSFVSVVPFEA"
     gene            complement(1499..1810)
                     /gene="rplU"
                     /locus_tag="PKN22_05420"
     CDS             complement(1499..1810)
                     /gene="rplU"
                     /locus_tag="PKN22_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNS42179.1"
                     /translation="MYAIVEIAGQQFKVSKDQKVFVHRLDAKEGDKLTFDRVLLVDND
                     GKIAVGAPAIEGANVTAEVVEHVKGDKVIVFKKKRRKGYQKQNGHRQCFTAIKVTGIK
                     A"
     gene            2415..3956
                     /locus_tag="PKN22_05425"
     CDS             2415..3956
                     /locus_tag="PKN22_05425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS42180.1"
                     /translation="MTDATGKTYVQEHYWSHLVAKIILFIYGAFLLLIFFYSLLQLSL
                     AIAYKKSKKTAKKQAPVYNPETTPKVTVQLPMFNEMYVATRIIDAAAAFDYPKDKLQI
                     QVLDDSTDETKDLIAKKVAEVAATGVNIQHIHRVDRTGYKAGALDAAMDRVEGDFIAI
                     FDADFIPEPDFLQLTMPYFQDEKVGVVQTRWGHLNKDYSILTELQAFGLNGHFAIEQG
                     GRNSAGHFINFNGTGGIWRKKCIEDAGGWEHDTLTEDLDLSYRAQMKGWKFQYLEDVV
                     SPAELPITMSALKSQQHRWMKGGVECFKKMSGRLLTAKGVRFTDRIHGISHLFNSSIF
                     LFILVVAILSLLILQVKDSFSDLNVILQFMGIFILSTIFLMVYYWYSFRDRTPEGKKS
                     FPKFAWRFLQFLVMSMGLSINNSVAVIEGYLGIKSSFVRTPKFNIAKKSEFKQNKYDK
                     KKINLVSVIEGVLSVVFLFTAVHRAAYGDLGMVPFHLMLGIGYGVVFYHSVKEVREAN
                     KAARG"
     gene            complement(4068..>4943)
                     /locus_tag="PKN22_05430"
     CDS             complement(4068..>4943)
                     /locus_tag="PKN22_05430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS42181.1"
                     /translation="IKQLKSGVDVLVATPGRMFDLIAQGHLDVSGVQHLILDEADLML
                     SKGFNKDIHDILKKIPHKRQTLFFSATIDKKIKALAYDVVKNAIRIQISPKNPVAKSV
                     EHAVAFIEMDDKRFFLENILAEYPDKRILVFVRTKVRAARVVAAMKRVGIETEEIHGG
                     IDQKERFQILDRFRKGENLVLITTDVSARGIDIPNVDYVINYDIPDDPENYVHRCGRT
                     GRGEKNGQAISFCSTSEKELLTAIEEYTGEEIAHYDIHPDDYKEILEDTEDLTYNWQK
                     LIDKDNQENGTEDEW"
BASE COUNT         1518 a          820 c          912 g         1693 t
ORIGIN      
        1 cacaattaat tgttggtgaa atgaattttg catttgaaca ccgtgttgca ccacaatcaa
       61 gtttggaagt ttcttttgga ccaacggttt ctgaaattgg aattaataaa atttattttg
      121 ataacgttct aaacaacaac aatgcaattt atggtttaaa ctcaagaaca caaagtggat
      181 tgggcttttt cttgggttta tcttaccgtt attatcctat ttctggagca tcaacagcgc
      241 caagaggatt gtatttagga ccagaaatta aatacagaat gtacaacacc tattacattg
      301 ataatttagg tgttttagaa aatagaacag gttctgccaa ccaattgttg ttcagatttc
      361 atacgggtta tcaatttgtg ataggtaaga aatttgcaat tgatgtgttt acagcattag
      421 gattaggcta cacagtgatt aatgcttatt acgatattca attatacaac tcgcaaacag
      481 gaggctttac aacaagttgg tattcaaaaa ctagaaatag agtatttgtt tcgggttctg
      541 ttggtgttaa atttgggttg ggaggaaatc gtaaagagta attctaggag tgatttttgt
      601 cactttttta attcaaataa tttattacat ttgccgataa ttaaattagc aagttaaaaa
      661 ttgaaaataa aagtagagtt atgagtatac cagcaaattt gaaatatacg aaagatcacg
      721 agtggattcg tattgaaggt gaaacagcag tagtaggaat cacagatttt gcgcaaagcg
      781 aattgggtga tatcgtatat gtagaggttg aaacagaagg tgaaacaatt gaacaaaacg
      841 aagtatttgg ttctgttgaa gctgttaaaa cagtttctga tttgtttttg cctgttacag
      901 ctgaaatttt ggaattcaac accaacttgg aagccaaccc agaattggta aactctgatc
      961 cttatggtga aggatggatt gtgaaaatta aaatcgctga cgcatctcaa attgatagtt
     1021 tattggatgc tgctgcatac gaagcagtta ttggataaat aaaatttgtg atttaaaata
     1081 gtaaaatcca ctcattgtag tggatttttt tgtgcctata tatttaaaat ttaatggata
     1141 aaaaaaatag ctacctcttg gagatagcta tttagttcaa tttcgtttat tcaattatgc
     1201 ctcaaaagga acaactgaaa caaatgaacg attgtctttt ttcttgcgga attctacgat
     1261 accatctgtt aatgcgaaca atgtatgatc tttaccgatt cctacatttt caccagcatg
     1321 gtgttgtgta cctctttgac gaacgatgat gtttcctgca attgcagctt gaccaccaaa
     1381 aatcttaacg cctaatctct tgctgtgaga ttcacgaccg tttttagaac taccgactcc
     1441 tttcttgtgt gccatttctt ttaattttta tattggattg ctccaatgta atttctaatt
     1501 acgctttaat tccagttacc ttaattgctg tgaagcattg acggtgaccg ttttgttttt
     1561 ggtaaccttt tcttcttttc tttttgaaaa cgattacttt atctcctttt acgtgctcta
     1621 caacttccgc tgtaacattt gcaccttcta tagctggggc gccaactgca atttttccat
     1681 cgttgtcaac taaaagaacg cgatcaaatg ttaatttgtc tccttctttc gcatccaaac
     1741 ggtgaacaaa taccttttgg tcttttgaaa ctttaaactg ttgccctgct atctctacaa
     1801 ttgcgtacat atagcttact tttttaatta atttctaaaa atttggacag caaagatata
     1861 taaaaaaagt aagatatcaa caattgatgg taaatatttt aatttctttt gaagaaagcc
     1921 cttaaattaa ggaaaatggt tgttaataat tgactatttt gcaaatataa tttacagaaa
     1981 tgcactatga atttattttt gaataacttt gcgctttctt aatacttttt tgatatatca
     2041 agtgtatgca taagttattg ttaatcacgc tattaattgg aatagcaacc ccttcttttt
     2101 ctcagtcatc taaacttgtt aaaggtagtt tggtggacga acgcacaaaa gatattgttg
     2161 aaaaagtaac tgtcgtcatt caaaatgtgg aaacaaaaga ttcttttgtt actgttgcta
     2221 ataaagatgg tgttttccaa tttaaaaata taccttttgg tgaatatgaa tttaaaatta
     2281 atgattctga atacaaaaga gacagtactt ttaactttac attggaagaa gcgcatgctg
     2341 ataattttac ttttggacaa ccaattgacg taaaacttaa agtatctcgt atttttaact
     2401 ggggcgaccg catgatgaca gatgcaaccg gtaaaactta tgtacaagag cattattggt
     2461 cgcacttggt ggcaaaaatc atcttattta tttatggtgc attcttgctg ttgattttct
     2521 tctacagttt attgcaatta agtttggcga ttgcatataa gaaaagtaaa aagacagcca
     2581 aaaaacaagc gccagtttac aaccctgaaa caactccaaa agtaacggtt caattgccaa
     2641 tgtttaatga aatgtatgtg gcaacgcgta ttatcgatgc agctgcggct tttgattatc
     2701 cgaaagataa attgcaaatt caggtgttgg acgattctac cgatgaaaca aaagatttaa
     2761 ttgctaaaaa ggtggcagaa gttgccgcaa ctggtgtaaa tattcaacac attcaccgtg
     2821 tggatagaac tggttataaa gcaggtgcct tggatgcagc aatggataga gtagaaggcg
     2881 atttcattgc tatatttgat gccgatttta tcccagaacc tgatttcttg caattgacca
     2941 tgccatattt ccaagatgaa aaagttggtg tggttcaaac tcgttgggga cacttaaata
     3001 aagattactc cattttgact gaattgcaag cctttggttt gaacggccac tttgctattg
     3061 aacaaggagg tagaaactca gctggtcatt ttattaactt taatggaact ggcggtattt
     3121 ggagaaaaaa atgtattgaa gatgctggtg gctgggaaca cgatacatta accgaagatt
     3181 tggacttaag ctaccgcgcg caaatgaaag gttggaaatt ccaatacttg gaagacgtgg
     3241 tttctcctgc cgaattgcca attactatgt cggcacttaa aagccaacaa caccgctgga
     3301 tgaaaggtgg ggttgagtgc ttcaaaaaaa tgtcgggtcg tttgttgaca gccaaaggag
     3361 ttcgttttac agacagaatt cacggtattt cgcatttgtt taactcttcg atctttttat
     3421 ttattcttgt tgtggctata ttgagcttgt taattttaca agtaaaagat agtttctctg
     3481 atttgaatgt catcttgcag ttcatgggaa tcttcattct cagcaccatt ttcttaatgg
     3541 tttactattg gtattcgttt agagatagaa caccagaggg taaaaaatcg tttccaaaat
     3601 ttgcatggcg tttcttgcaa ttcttggtaa tgtctatggg actttcaatc aacaactcgg
     3661 ttgccgtaat tgaaggttac ttaggaatca aaagttcgtt tgttcgtaca cctaaattta
     3721 atattgctaa aaaatctgaa ttcaagcaaa ataaatacga caagaaaaaa atcaatttgg
     3781 taagtgttat cgaaggtgtt ttgtctgttg tgtttttatt cactgccgtt cacagagcag
     3841 cttatggcga tttaggaatg gtgccttttc acttaatgtt gggaattggt tatggggttg
     3901 ttttctacca ctctgttaaa gaagtaagag aagcaaataa agctgctaga ggataagctt
     3961 tttatagtat tttgatattg taccactgat tgttataaaa tcagtggtat ttttttgtct
     4021 caattaggat tattacacga tgattctttc tactaaatag ttcagaatta ccactcgtct
     4081 tctgtgccgt tttcctgatt gtctttatca attaattttt gccagttgta ggtcaaatct
     4141 tctgtgtcct ccaaaatttc tttgtaatca tcaggatgga tatcgtaatg cgcgatttct
     4201 tcaccagtat attcttcaat ggctgttaac aattcttttt cagaagttga acaaaaacta
     4261 atggcttgac catttttttc accacgtcca gttcgcccac agcggtgcac atagttttct
     4321 ggatcatctg gaatatcgta attgataacg tagtcaacat ttggaatatc aatcccacga
     4381 gcagatacgt cggtggtaat caaaaccaaa ttttcacctt ttctaaatcg atctaaaatt
     4441 tgaaaacgct ctttttggtc tatgccacca tgaatttctt cggtttcaat tcccactcgc
     4501 ttcattgccg ccacaacgcg tgcggctctt acttttgtgc gcacaaacac caaaatacgt
     4561 ttatcgggat attctgcaag aatgttttct aagaaaaagc gtttatcatc catttcaata
     4621 aaagcaacgg catgctccac cgattttgct actggatttt tgggcgatat ttgtatgcga
     4681 atggcatttt ttaccacgtc ataggccaat gctttgattt ttttatcaat tgttgctgag
     4741 aaaaacaagg tttgacgttt gtgtggtatt ttctttaaaa tatcgtgaat atctttgtta
     4801 aaccctttgg aaagcatcaa atcggcttcg tcaagaatta aatgttgcac tcccgaaaca
     4861 tctaaatgtc cttgagcaat caaatcaaac attctaccag gcgttgccac taaaacatcc
     4921 acaccacttt tcaattgctt gat
//
LOCUS       DAOQVQ010000136         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_18898_length_4942_cov_4.433599, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000136 DAOQVQ010000000
VERSION     DAOQVQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18898_length_4942_cov_4.433599"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKN22_05435"
     CDS             complement(<1..649)
                     /locus_tag="PKN22_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NorD protein"
                     /protein_id="HNS42182.1"
                     /translation="MSLDEYLFGKYVRYLKNKKPSEAVIQRTVELKPIQQKLTLIARA
                     MTGLPIEIFTAVKQGGYKGDNFFLPEKFNFYENADQNLAYYLFRTLYLCQQQKMQLNW
                     FETSEIAEEKAIEIAKNNSQAILAALIEEFPSIYEVYEDLVIAKNPESKEAIDTSWLY
                     GRVMKDDLLAKNKQEDFQNNALQNNENPDPKTILKARAVEEIKNLMNIHFFVTTQK"
     gene            complement(653..1456)
                     /locus_tag="PKN22_05440"
     CDS             complement(653..1456)
                     /locus_tag="PKN22_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNS42183.1"
                     /translation="MEAMIEKQPTTVVPFYQETGKEVEIFKQTYLNKIPLLLKGPTGT
                     GKSRFIEHMAAQLNKQLITVSCHEETSATDLIGRFIIKGAETVWMDGPLVTALKTGAI
                     LYLDEIAEARPDVIVAIHPLTDHRREIFIDKLGTSVKAHPDFMLVASFNPGYQKGYKE
                     LKPSTRQRFAAISFQYPNAQTEASILVKETGIDEDTAKKLVKIAEKIRNLKELGLTET
                     VSTRLLVDAAFLIKSGLPKRLSVKTAVIEPLTDELEIIDALVDLSNLII"
     gene            complement(1529..2878)
                     /locus_tag="PKN22_05445"
     CDS             complement(1529..2878)
                     /locus_tag="PKN22_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNS42184.1"
                     /translation="MKYKSQKIAYYFFALALLLLVLQIVYGFIMGFARIGYDGLHEFI
                     PFNTARAVHTNLLVVWLLTGFMGAAYYIIPEEAEKELVSVKLAYVQLISLAIVGVIAI
                     VGYHFNYWEGRKFLEIPRPLDYLVVVNVLLFLGLILATLYKGKKRTTTAMVLTMGLVF
                     AALLYLPGMIWFDNQTVDSFFRWWVVHLWVEGVWELILGGILAFLLIKLTGVDREVIE
                     KWLYVIIGLTFISGIIGTGHHYYFIGTGSIWIILGGIFSALEPFAFLAMVFFTIGMYR
                     KGEKKHPNKIALFWTVGTAVVAFLGAGMLGLAHTLPGVNRYTHGTLVTAMHGHLAFWG
                     AYAMIVFAFITYVMPNLTGRKLYESTNGKLAFWLSNIGMFGMTAAFGVAGVAQVYLER
                     KMGMEFGDVQNEIKVHFYVLIACATLFLSGVSIFVWEFIKYGKPNDEALITENGIEE"
     gene            complement(2889..3329)
                     /locus_tag="PKN22_05450"
     CDS             complement(2889..3329)
                     /locus_tag="PKN22_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNS42185.1"
                     /translation="MMLSKKNAKVFFFGGTIVTFAMFLFMSYATLTNEVPKRTHEENL
                     SAAVIEGKKIWEENNCMGCHTIMGEGGYYAPELTKVFERRGEGFIKAVLQSPSPWQPR
                     GRKMVAYNMSDEDAANMVAFLKWVGEMDLNGFPPKPDYKSTINK"
     gene            complement(3496..4188)
                     /locus_tag="PKN22_05455"
     CDS             complement(3496..4188)
                     /locus_tag="PKN22_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HNS42186.1"
                     /translation="MENYLDNHYIHRSNWLRAAVLGANDGIISVSSLAIGVAAASSDS
                     DAIILATFAGVVAGALSMAAGEYVSVSSQTDIEKADIAREAKELEEEPEEELKALAYI
                     YEQRGLKKETALQVAKELTENDALAAHVRDELGITEISQANPIQAALASGASFLAGGA
                     LPLLVTFIAQPTHIEYWIYGSTILALMLLGAFSAKTGGAIVSKAVVRVTVWGTIAMGL
                     SAAVGFLFGVTI"
     gene            complement(4238..>4942)
                     /locus_tag="PKN22_05460"
     CDS             complement(4238..>4942)
                     /locus_tag="PKN22_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS42187.1"
                     /translation="TVPMLVYLFGFNAIAAVTYSFFIVGTTSLVGSYAYYKKDLLDFK
                     TAFVFGIPSILAIFITRFLILPAIPATINISDGLQLSKEYALLLLFAIVMVWAAWNML
                     KKKKNDALVETSVVTIFTQGAFVGFLTGLTGAGGGFLIIPVLVNVLHVPMKKAVGTSL
                     FIIAVNSLLGFVSSLSYAAIDWAMLLSISLIAVVGVFVGSYLSNKIDGNKLKPAFGWF
                     VLATGAIILVKEILLK"
BASE COUNT         1539 a         1011 c          826 g         1566 t
ORIGIN      
        1 ttttttgagt agttacaaaa aagtgtatgt tcattaaatt tttaatttct tcaacagcgc
       61 gtgcttttag aatagttttg ggatcaggat tttcattgtt ttggagtgca ttgttttgaa
      121 aatcttcttg cttgtttttt gccaacaaat cgtctttcat tacacgacca tacagccaag
      181 aagtgtcgat tgcttctttg gattctggat tttttgcaat gaccaagtct tcatacactt
      241 cgtaaatgga tggaaattct tcgatcaaag ctgctaggat ggcttgtgaa ttgttttttg
      301 ctatttctat tgctttttct tcggcaattt ctgaggtttc aaaccaattg agttgcattt
      361 tttgttgttg gcacaaatac aaggtgcgga acagataata ggctaaattt tgatccgcat
      421 tttcgtaaaa attaaacttt tcgggcaaga aaaaattatc acctttgtag cctccttgtt
      481 tcactgccgt aaaaatttca atgggcaaac ctgtcattgc acgtgcaata agtgtcaact
      541 tttgttgaat gggttttaac tcaaccgtgc gttgtattac tgcttccgaa ggttttttgt
      601 ttttcagata gcgcacatac tttccaaaca ggtattcatc aagtgacatg acttaaataa
      661 ttaagttcga caaatcaacc aaggcatcaa taatttccaa ttcatcggtt aaaggttcaa
      721 taaccgcagt tttaacgctt aatcgtttag gtagtcccga tttgattaaa aaagctgcat
      781 ccaccaacaa acgagttgaa acagtttctg tgagtcctaa ttcttttaga tttctaattt
      841 tttcggcaat tttcaccaat tttttagccg tatcttcatc aattccagtt tcttttacta
      901 agatagaagc ttctgtttgt gcatttggat attggaatga aatggctgca aaacgctgac
      961 gagttgaggg tttcaattct ttgtatcctt tttgataacc tggattaaac gatgctacca
     1021 acataaaatc aggatgtgct ttaacacttg ttcccaattt atcgataaaa atttctcgac
     1081 ggtggtcggt caatgggtga atggcaacaa tcacatctgg gcgtgcttct gcaatttcat
     1141 ccaagtataa aatagctcct gttttgagtg cagtaaccag tggaccgtcc atccaaactg
     1201 tttctgcccc tttaatgata aatcggccaa ttaaatcggt tgctgatgtt tcttcgtgac
     1261 aactcactgt tattaattgc ttgtttaatt gcgctgccat gtgctcaata aaacgcgatt
     1321 ttcctgttcc agttggacct tttaaaagca atggaatttt attgagatag gtttgcttaa
     1381 aaatttcaac ttctttccct gtttcttggt aaaaaggtac aacagttgtt ggttgttttt
     1441 caatcattgc ttccatattt tttatgtttt aaactaaatc ctgagttgct tttacacaac
     1501 ccaggattaa tagttggtaa ttgtttattt attcttcaat tccgttctca gtaattaaag
     1561 cttcatcatt tggttttcca tatttaatga attcccaaac aaagatgcta acacccgata
     1621 agaacaaagt tgcacaagcg attaacacat agaaatgcac tttaatttcg ttttgtacat
     1681 cgccaaattc catccccatt tttctttcca agtaaacttg agcaacacct gcaacaccaa
     1741 aggcagcagt cattccaaac attccgatgt tggacaacca gaacgctaat ttgccattag
     1801 ttgattcata caattttcta cctgttaaat tcggcatcac ataggtaatg aatgcaaaaa
     1861 caatcatagc ataagcaccc cagaaagcta agtggccgtg cattgcagta accaatgttc
     1921 cgtgtgtgta acggttaacc cctggtaaag tgtgcgccaa tcctaacata ccagcaccta
     1981 agaaagccac tactgctgtt ccaactgtcc agaacaacgc aattttattt ggatgttttt
     2041 tctcgccttt gcggtacata ccaattgtaa agaacaccat tgcaaggaaa gcgaaaggct
     2101 ctaatgcgga gaagatacca cctaagatga tccaaatgct accagttccg atgaaatagt
     2161 agtggtgacc tgtaccgata attccagaaa taaatgttaa accgataatt acatacaacc
     2221 atttttcaat tacttctcgg tcaacaccag ttaatttaat caataagaat gctagaatac
     2281 cacccaagat taattcccaa acaccttcaa cccataagtg gacaacccac caacggaaga
     2341 aagaatccac tgtttggtta tcaaaccaaa tcataccagg caagtaaagc aaggctgcaa
     2401 acaccaatcc cattgttaat accattgctg tagtggtgcg ttttttacct ttgtataatg
     2461 tggctagaat taatcctaag aacagtaaaa cattgaccac aacaaggtaa tccagaggtc
     2521 gaggaatttc taagaattta cgaccttccc agtaattgaa atggtaacct acaattgcaa
     2581 taacgcccac aattgccaaa gaaattaatt ggacataggc tagtttaacg cttaccaatt
     2641 ctttttctgc ttcttctgga atgatgtaat aggcagctcc cataaaaccg gtgagcaacc
     2701 aaactaccag caagttggtg tgaaccgcac gagctgtatt gaaaggaata aattcatgca
     2761 atccatcgta accaatacgt gcgaatccca tgataaatcc gtaaactatt tgcaatacca
     2821 acagcaaaag tgccaaggca aagaaataat atgctatttt ctgacttttg tatttcataa
     2881 cctcgatttt atttgttaat agttgatttg taatcaggtt ttggtgggaa accgttcaag
     2941 tccatttcac ctacccattt taaaaatgcc accatgtttg cagcatcttc atcgctcatg
     3001 ttgtaagcca ccattttgcg cccacgaggt tgccaagggg aaggcgattg caacacggct
     3061 ttaataaatc cttcgcctct tcgttcaaac acttttgtaa gttcaggcgc atagtaacca
     3121 ccttcaccca taatggtgtg acagcccatg cagttgtttt cttcccagat tttcttgcct
     3181 tctataacgg cagcactgag attttcttca tgcgttcttt ttggaacctc atttgtcaat
     3241 gttgcatacg acatgaacaa aaacatggca aatgttacta tggttcctcc aaagaaaaac
     3301 actttcgcat tttttttaga taacatcata atttttaaaa ttttaaataa acaattcact
     3361 ctagattgtc ttaggtgtta ccacgtatga caaatctatg acaaatgtaa taaaggacaa
     3421 ttttgtcctt tatgattaaa atcactggaa ttgttatttt tttttactac atttgtagct
     3481 ttccttaaaa tttcattaga ttgttactcc aaaaaggaaa cctacagcgg cagaaagtcc
     3541 cattgcaatt gttccccaaa ctgtcactct tacaacagct ttgctcacaa tagcaccacc
     3601 tgttttagct gaaaaagctc ctaatagcat taatgcgagt atggttgaac cataaatcca
     3661 atattcaata tgtgttggtt gtgcaataaa tgttaccaac aaaggcaacg ctccgcctgc
     3721 taaaaatgat gctcctgatg ccaacgcagc ttgtatgggg ttggcttgag agatttctgt
     3781 gatacctaat tcatcgcgta catgtgctgc cagcgcatca ttttctgtca gttcttttgc
     3841 tacttgtaga gcagtttctt ttttcaaacc ccgttgttcg tagatgtagg ctaatgcttt
     3901 taattcttct tcgggttctt cttccaattc ttttgcttcg cgagcaatat ctgctttttc
     3961 aatatcggtt tgagagctaa cggaaacgta ttctccagca gccattgata aggcgcctgc
     4021 cacaactcct gcaaaggttg ctaaaattat tgcatcacta tcgctacttg ctgcagcaac
     4081 accaattgcc aaactagaaa cagagataat tccatcgttt gcaccaagaa ctgcggctct
     4141 taaccagttg cttcggtgaa tgtagtgatt gtctaaataa ttttccattg catgtaattt
     4201 atcagttgaa ggtaaatata aaaaaatgtt tcaactttta tttcaataag atttctttca
     4261 ccaatattat ggctccagta gctaatacaa accatccaaa agcaggtttc aatttgttac
     4321 catcaatttt attggataaa tagctaccta caaaaactcc aaccacagca atcaatgaaa
     4381 tggacaaaag cattgcccaa tcaattgcag cataactcaa tgaagataca aaacctaaca
     4441 aggaattaac ggcaatgata aacaacgacg ttccaactgc ttttttcatc ggcacgtgca
     4501 acacgttaac caaaacagga ataataagaa atccacctcc agctccggtt aaaccagtta
     4561 aaaaacctac aaatgcacct tgtgtaaata tcgttactac tgacgtttca acgagggcat
     4621 cgtttttttt ctttttcaac atgttccaag cagcccaaac cattacaatt gcaaacaaaa
     4681 gaagcaaggc atattcttta gatagttgta agccatcaga aatgtttatg gttgctggta
     4741 tagcaggcaa aataagaaag cgtgtaataa aaattgctaa tatagaagga ataccaaaaa
     4801 caaatgccgt tttgaaatcg agtaaatctt ttttgtaata ggcatacgaa ccaaccaagc
     4861 tagttgttcc cacaataaaa aaagaataag ttacagctgc aattgcatta aaaccaaata
     4921 aatataccaa cattggaaca gt
//
LOCUS       DAOQVQ010000137         4859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_19331_length_4859_cov_4.338052, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000137 DAOQVQ010000000
VERSION     DAOQVQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19331_length_4859_cov_4.338052"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKN22_05465"
     CDS             <1..279
                     /locus_tag="PKN22_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42188.1"
                     /translation="LAFFRPIIFKAFSPRDGYDTNKEPSIYEMGNRWFINCFGILLIL
                     HHSWFFVLEVFKFSEFLFVLKKIVLSVPVSYLMALLLQFLFIRVKPTK"
     gene            357..2147
                     /locus_tag="PKN22_05470"
     CDS             357..2147
                     /locus_tag="PKN22_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNS42189.1"
                     /translation="MQVVDDQWKLRAQEIAEKRKYIVPPRAAMFDRNGKKVVSNKAYY
                     NLMFVENDIKDLDTAAFGKLVGMTVEEVRNRFKEIREKEGYFKRKDGKRVPNYQPVRP
                     YAFLKELTADEISKIAPNLDKFPGFYEQVTSMRSYPYAGGANILGYLSEVNQQEIDED
                     RFYRSGDNIGKAGVEKYYEKELRGRKGVKYIVTSALNNVIESYANGKYDTLALQAEPL
                     KLGMDIDLQVYGEQLMQNKMGCIVAIDPNSGEILAMVSSPSYDPNLFVGRSNISKNYP
                     RLLMDPKKPLFPRPLQAEYPPGSIFKLTQALIGLQEGVITPNTSFPCNQSQVGCHNHP
                     TAQNVAQAVQMSCNPYFYGVTRKIIQQGKSKNAFKDAEIGLGLWAEYLHSFGLGIKLD
                     VDVTGLRSGLIPDPNYYNNLFPSKRHQYGKHRWAFSTIRSNSIGQGEVKLTPLQMANL
                     AVIMANRGWFYTPHIVKSVGGKGPLPQFSIKHKTMVDAKHFAPVIEGMRMAVNEGGGT
                     ARMARINGITVCGKTGTAQNPHGKDHSVFIAFAPMDKPKIAIAVFIENSGFGGTWAAP
                     IASLMMEKYINGVVKDKEKEKYILEAVLEK"
     gene            2144..3550
                     /gene="rodA"
                     /locus_tag="PKN22_05475"
     CDS             2144..3550
                     /gene="rodA"
                     /locus_tag="PKN22_05475"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNS42190.1"
                     /translation="MMRENEKITSYIDWPLLITYFLIMSLGLMTVYSVGYDEETNMSF
                     SFSTLFGRQLIWVGVSLFLGLLVFLIDSEIYRKFSIPIYAGTLFLLVVVLFTPPINGA
                     RAWLGIGPFGIQPAEFAKIGTAILLASYIDKINIRLQTIPNILYCLAIIAIPMVLIML
                     QPDAGTFVVFTSFFFILYREGITFDPIVLSIINSFPGMKFKQTWIGTHFIPILFVVVF
                     ISVVTLFMSKTTFDFEILGLYEVPSIAVIIAVYFLFAILFYLIGRNFISSRNRKQFLI
                     VISLTFIIFSTLSASVSTAFNSLKQHQKNRIELLLGLNEDDQRDGEDYNRNRAMTAVG
                     SGGFLGKGYTKATLSSIKSNHVPESDTDFIFCPFAEEWGFVGALIIVGLYIFFLLRII
                     TIAERQRSTYNRVYAYSVGMILFYHFAINIGMNIGLVPIIGIPLPFFSYGGSSMMSFS
                     VMFFILLKLDSQRRDTLF"
     gene            3651..4613
                     /locus_tag="PKN22_05480"
     CDS             3651..4613
                     /locus_tag="PKN22_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS42191.1"
                     /translation="MKKSILIISISLIAASCVPAKKYNDLLASNKECNDELALYKSKA
                     LSFEGKSTELEAQQAVLTKELDQLKSDTTRLGNEFRSLQAQYDKLTERVETLETSFDK
                     YRLSGEKKSALLQADLDAKSLELQRKQDELSELEAELKNKQQLLAERERRVNELEEMI
                     AKQEQATKALKQKIADALKGYSNQGLSVYEKNGKLYVSLEAKLLFASGSVVVDGAGKK
                     ALVDLSKIIENEKNIEIIVEGHTDTDKLASGSHPKNNWELSVLRSTAVIDILLANSKI
                     DPKILVASGRSEYLPVDIADKSKNRRIEIIIAPNLNELFDLINK"
BASE COUNT         1596 a          773 c          918 g         1572 t
ORIGIN      
        1 cttgctttct tccgtcctat tatatttaaa gcatttagcc ctagagatgg ttatgatact
       61 aataaagaac catccattta tgaaatgggt aaccgctggt ttattaattg ttttggtata
      121 ttgttgattt tacaccattc ttggtttttt gttttagaag tatttaagtt tagtgaattc
      181 ctttttgtgc ttaaaaaaat tgttttgagc gtaccagttt cctacttaat ggcattactt
      241 cttcagtttt tattcatccg tgttaagcca acaaaatgac atttgacggt agaaaatata
      301 ttgtcatttt tttcatctca atagtcggta ttatttacgt attccgattg tttcacatgc
      361 aagtggttga tgatcaatgg aaattaaggg cacaagaaat tgcagaaaaa agaaaataca
      421 ttgttcctcc acgtgctgca atgtttgata gaaacggaaa aaaagtcgtt tccaataaag
      481 catactacaa cttgatgttt gttgaaaacg atattaaaga tttggacaca gctgcctttg
      541 gtaagttggt tggaatgacg gttgaagaag tgcgtaatcg ttttaaagaa attcgcgaga
      601 aagaaggtta ttttaaacgt aaagacggaa aacgagttcc caattaccaa cctgttcgcc
      661 cttatgcttt tctaaaagag ttaacagcag atgaaatttc taaaattgcg ccaaatttgg
      721 ataaattccc tggtttttac gaacaagtta ccagcatgcg ctcttatccc tatgccggtg
      781 gagcaaatat cttaggttat ttatccgaag tcaaccaaca agaaattgat gaagaccgtt
      841 tttaccgttc tggagataat atcggtaagg cgggagttga aaaatattac gaaaaagaat
      901 taagagggcg caaaggtgtt aaatacatcg ttacttctgc cttaaacaat gtaattgaat
      961 catacgccaa cggaaaatac gatactttag cattgcaagc cgaacctttg aaattaggga
     1021 tggatattga tttgcaagta tatggcgaac aattgatgca aaataaaatg ggatgtattg
     1081 tcgcaattga tcctaactct ggagaaattc tagcgatggt ttcctctcca tcctacgacc
     1141 ctaatttatt tgttggaaga tccaatattt caaaaaatta tccacggttg ttaatggatc
     1201 ccaaaaaacc attgtttcca cgtccattgc aagcggaata ccctcctggt tcaattttta
     1261 agttaaccca agcattaatt ggtttgcaag aaggagtcat tactcccaat acctcatttc
     1321 cttgtaatca aagtcaagtt ggttgccata accaccctac ggctcaaaac gtagcacaag
     1381 cagtgcagat gtcgtgtaac ccttattttt atggggtaac tcgcaaaata attcaacaag
     1441 gtaagagtaa aaatgcattt aaagatgccg aaataggact cggattgtgg gcagaatatc
     1501 tacattcttt tggtcttgga atcaagttgg atgttgatgt aacaggactg cgttctggtt
     1561 taattccaga ccccaactat tacaataatt tattcccgtc aaaacgtcat caatacggga
     1621 aacaccgttg ggcattttca accattcgat ccaactctat tgggcaagga gaagtaaagc
     1681 taacaccctt acaaatggct aatttagcag tgattatggc caatagaggt tggttttata
     1741 caccacatat tgttaaatca gttggaggaa aaggtcctct tcctcaattc agcatcaagc
     1801 acaaaacaat ggtagatgcc aagcattttg cacctgtaat tgaaggaatg agaatggctg
     1861 taaatgaagg tggaggtact gctaggatgg cacgtataaa tgggataaca gtatgtggta
     1921 aaacgggtac ggcacaaaac cctcacggaa aagaccactc tgtattcatt gcttttgcac
     1981 cgatggataa gcctaaaatt gcaattgccg tttttattga aaattctggt tttggtggaa
     2041 cttgggcagc tccaattgct agtttaatga tggaaaaata catcaatgga gtagtaaaag
     2101 acaaagaaaa agaaaaatac attctagaag cagtgttaga aaaatgatga gagaaaatga
     2161 gaaaataaca agttatattg attggccttt gctaatcacc tattttctta tcatgtcttt
     2221 gggtttgatg acagtatatt ctgtagggta tgatgaagaa accaacatgt cttttagttt
     2281 ttcaaccttg tttgggcgtc aattaatttg ggttggagtc agtttgttcc ttggattact
     2341 cgtgtttcta atcgactctg agatttaccg caaattctcc atccctatat atgcaggtac
     2401 tttgtttcta ttggttgttg ttttgtttac acctccaatt aatggtgcta gagcttggtt
     2461 aggaatcggt ccttttggta tccagcccgc tgagtttgcg aaaattggta cagcgattct
     2521 tttggctagt tacattgata aaatcaatat tcgcttacaa accattccta atattttgta
     2581 ttgtttggca attattgcca ttccaatggt gttgattatg ttgcaacccg atgcgggaac
     2641 gtttgttgtt ttcacatcgt ttttctttat cctttatcgc gaaggaatca catttgatcc
     2701 aattgtactg tcaattatta attcatttcc aggaatgaaa tttaaacaaa catggattgg
     2761 tactcacttt attcctattc tatttgtagt tgtttttatc tctgttgtta ctctttttat
     2821 gagtaaaaca acatttgatt ttgaaatttt agggttgtat gaagtgcctt ctattgctgt
     2881 aattatcgca gtttattttc tttttgcaat attgttttat ctaattggga gaaattttat
     2941 tagtagtaga aatagaaaac agtttttaat tgtaatctcc ttaactttta taattttttc
     3001 aacgctttct gcatcggttt caacagcttt caactcttta aaacagcacc aaaaaaatcg
     3061 aattgaatta ttgcttggtc tcaatgaaga tgatcaacgg gatggagaag attacaaccg
     3121 taatagggct atgacggctg taggatcagg agggttttta gggaaaggat atacgaaagc
     3181 aactttatca tcaattaaat ccaatcacgt cccagaaagt gataccgact tcattttttg
     3241 cccctttgca gaagaatggg ggtttgttgg tgcattgatt attgttgggt tgtatatatt
     3301 tttcttattg cgaatcatca caattgcaga acgccaacgc tccacttata accgggttta
     3361 cgcttattcg gtgggaatga ttttatttta ccactttgca attaacattg ggatgaacat
     3421 cggattagtg ccaattattg gtattccact gccatttttc agctatggag gctcatccat
     3481 gatgtccttc tcagtaatgt tttttatcct tctaaaatta gatagtcagc gaagagatac
     3541 tttattctaa tcgtctcata atagaattat tcaactttta gtgttaacaa gtaattgaat
     3601 gttgaatttt tttcatttaa aaagtagcga attttgtaaa aaacgtgaaa atgaaaaaaa
     3661 gcattctaat tatttccatt tctcttattg cagctagctg tgtgcctgca aaaaaataca
     3721 atgatttatt agcaagcaat aaagaatgca acgatgaatt ggcattgtat aaaagtaaag
     3781 cactttcttt tgaaggaaaa tcgactgaat tggaggcaca acaagctgtg ctaacaaaag
     3841 aattagatca actcaaaagc gatacaacgc gtttaggaaa tgaatttaga agtttacaag
     3901 cacaatacga taaattaaca gaacgagtag aaacacttga aacttcattc gataaataca
     3961 gattatcggg cgaaaaaaaa tcggcattac ttcaagccga cttagatgca aagagtcttg
     4021 aattacaaag aaagcaagat gagttatcag aattagaggc agaattaaaa aacaaacagc
     4081 agttgttggc agaacgtgaa cgtagagtaa atgaattgga agaaatgatt gcaaagcaag
     4141 agcaagcaac caaagcatta aaacaaaaaa ttgctgatgc attaaaagga tattctaacc
     4201 aaggtttatc ggtttatgag aaaaacggaa aactatatgt tagtcttgaa gccaaactat
     4261 tatttgcttc tggtagcgtt gtagttgatg gtgcaggtaa aaaagcattg gttgatttat
     4321 caaaaatcat tgaaaatgaa aaaaacattg aaattattgt agaaggacat acagatactg
     4381 ataaattggc tagtggtagc catcccaaaa ataattggga gttatctgta ttgcgttcaa
     4441 cagcagtaat tgatatttta ttagcaaatt ctaaaatcga tccaaaaatc ttagtagcat
     4501 caggcagatc agaatacctg ccagttgaca ttgctgataa atcaaaaaac agaagaattg
     4561 aaattattat tgctccgaac ttgaatgaat tattcgattt aatcaataaa taaaagaagt
     4621 tgaggtttaa aaagagcttt tttaactacc tgattcagaa ccaaattaaa attattttaa
     4681 aaaaaagttc aaaaaaaaac cagaatctat tttgaataaa gaatttttgt atatctttgc
     4741 aatccaattt tgaaagagtt ggtttttcaa aagttctttt atttattgat gtaccatcaa
     4801 gtacgccgat gtagctcagt tggctagagc agctgatttg taatcagcag gtcgtgggt
//
LOCUS       DAOQVQ010000138         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_19613_length_4808_cov_3.729434, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000138 DAOQVQ010000000
VERSION     DAOQVQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19613_length_4808_cov_3.729434"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..780
                     /locus_tag="PKN22_05485"
     CDS             28..780
                     /locus_tag="PKN22_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNS42192.1"
                     /translation="MKFKSDLFDLLEEKAKFYNQPSFISSDPVSIPHQFSRKEDIEIA
                     GFFAATIAWGNRQMIIKNGKKLMQLMEHAPHQFVLDYTPRDLEFVHRTFNAVDLDFFL
                     RALQHIYKNSDLEQTFTRQQPSDTIKDRIVHFRTTFLDLPHEKRSEKHLSNPLKNAAC
                     KRINMYLRWMVRPSQKGVDFGIWKSITPAELYLPLDVHTSRHARNLGLLTRKQDDWKA
                     LEEVMLHLRKLDANDPVKYDFALFGIGAFEQL"
     gene            931..4263
                     /gene="secA"
                     /locus_tag="PKN22_05490"
     CDS             931..4263
                     /gene="secA"
                     /locus_tag="PKN22_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNS42193.1"
                     /translation="MISNLLKAFLGDKSTKDRKEYQPYIDKTRTAQGQFKNLSDDELR
                     AKTRGFQTKIKESIQSLEQEVVELKEKVENPQLSISEKEAMYDKIDSMTKVIDDKIEE
                     VLLEILPEAFAVVVETARRWTENGQLVVTTQDFDRDLATKKDGITINGDTTIWHNKWT
                     AAGAEIEWKMAHYDVQLMGGAVLHTGKISEMQTGEGKTLVATLPVYLNALAGKGVHVV
                     TVNDYLAKRDSEWMGPLYQFHGLTVDCIDKHRPNSHERRNAYKADITFGTNNEFGFDY
                     LRDNMAGHPDELVQGKHHYAIVDEVDSVLIDDARTPLIISGPTPKGDEHEFHILKPRI
                     ENIVAAQRQLVNQCLADAKRMLTVIQDGFENKKDGKEMLEQGGIALLRAHRGLPKNKA
                     LIKFLSEPGIKAHLQKTENFYMQEQGKRMKEIDKELFFVIEEKQNTIELTDKGIDLIS
                     GQDDRNFFVIPDIGLQIAAISKENLPQEQFQAKKAEILREYSIKSERIHSINQLLKAY
                     TLFEKEVEYVVMDGKIKIVDESTGRIMEGRRYSDGLHQAIEAKENVTVEAATQTYATI
                     TLQNYFRMYHKLAGMTGTAETEAKEFWDIYKLDVVVIPTNKPILRKDQNDLVFKTARE
                     KFAAVIEETERLVAQGRPVLVGTTTVEISELLSRMLKMKGIKHNVLNAKQHQKEAEIV
                     AEAGIAGAVTIATNMAGRGTDIKLGEGVKEAGGLAIVATERHDSRRVDRQLRGRSGRQ
                     GDPGSSQFFVSLEDDLMRKFGSERIAKLMDRMGLKEGEVITHSMVSKSIERAQKKVEE
                     NNFGIRKRLLEYDDVMNAQRKAVYKKRRNALFGERLDVDIDNMLYDTAEAITMKNWKG
                     DFAVFEDELIRDLNIQSPVTPEEFATIDQVPLTEKVYMAMIRQYNEKNERIINLCWPQ
                     IKYVQENESHRYKTMAIPLTDGRRTMQIGVQLEEAYKAEGKNISKQLEKAIVLNIIDN
                     EWKEHLREMDELRSAVNNAQYEQKDPLLVYKLESFELFKNMHARLNKEAVELLMKLDI
                     EVDNKVEASTQEIRQENHYAKAKETVSSDIQNTRNDYQEAMESSHQQPVKREPVVVED
                     KIGRNDPCPCGSGKKYKQCHGK"
     gene            4359..>4808
                     /locus_tag="PKN22_05495"
     CDS             4359..>4808
                     /locus_tag="PKN22_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNS42194.1"
                     /translation="MIQTVGIIGSGNVAWHLAHALQALPTVKLTWIYSRNAQTLAELG
                     TELNVFTSTTLPSSAVDLILVCVNDDSIQTIVSQLPKDSNIAYTSGVVELNSIVFTQK
                     NYGVFYPLQTFTRKKEIALNEVPFLIEASNEAFTCELEQLAVLLSKNV"
BASE COUNT         1693 a          847 c          985 g         1283 t
ORIGIN      
        1 caatcaaaga aatggtactt ttgtgtcatg aaatttaagt ccgacctttt cgatttgctt
       61 gaagaaaaag caaaattcta taatcaacct tcttttatct catctgatcc ggttagtatt
      121 ccacatcaat tcagccgaaa agaggatata gaaattgccg gtttttttgc tgcaactatt
      181 gcttggggca atcgccaaat gattatcaaa aacggcaaaa aactcatgca attgatggaa
      241 catgcaccac atcaatttgt gttagattat accccacgcg atttggaatt tgtgcaccgt
      301 acgttcaatg ctgttgattt ggattttttc ctacgtgctt tgcaacacat ctataaaaat
      361 agtgacttag aacaaacctt tacgcgtcaa caaccttctg acaccatcaa ggatcgcatc
      421 gtgcattttc gtactacatt tttagacctt ccacatgaaa aacgttcaga aaaacacttg
      481 tccaacccgc tcaaaaacgc tgcttgcaaa cgcattaaca tgtatttacg ttggatggtg
      541 cgtccttctc aaaaaggagt ggattttgga atttggaaaa gcattacacc agcagaactt
      601 tatttgccgt tggatgttca caccagtcgc catgcacgca atttaggact tctcacacgc
      661 aagcaagacg attggaaagc cctcgaagaa gtaatgctcc atttaagaaa attagacgcc
      721 aatgaccctg taaaatacga ttttgccttg ttcggaattg gcgcatttga gcagttatag
      781 cattcttttc gctgacaatc tgtcattttt tcggctattc tcttacattg gaatgatttt
      841 agtcgcattc tttagcaaat atttaacacg aagtttatct ttaaaaacaa gaaataactt
      901 atttttgaca ctcttaaaaa ttaacgttca atgatttcaa acttattaaa agctttcttg
      961 ggagacaaat caaccaaaga cagaaaagaa tatcaaccat acattgataa aacaagaact
     1021 gctcaagggc aatttaaaaa tttatcggat gatgaattac gtgcaaaaac acgtggcttt
     1081 caaacaaaaa ttaaagaaag catccaaagt ttagagcaag aagttgttga actaaaagaa
     1141 aaagtagaaa atccgcaact ttctatttct gagaaagaag caatgtacga taaaatcgac
     1201 tcaatgacca aggtgattga cgataaaatc gaagaagttt tattggaaat tttacccgaa
     1261 gcatttgctg ttgttgttga aacggcaaga cgttggaccg aaaacggaca attggttgta
     1321 acaacacaag attttgaccg tgatttggca accaaaaaag atggaattac catcaatggt
     1381 gacacaacaa tttggcacaa caaatggacg gcagctggag ctgaaattga atggaaaatg
     1441 gcacactacg acgttcagtt gatgggtgga gctgttttac acaccggaaa aatctccgaa
     1501 atgcaaacag gggaaggtaa aaccttggtg gcaacacttc cagtttattt gaatgcactt
     1561 gcaggtaaag gtgtgcacgt ggtaactgtc aacgattact tggcaaaacg tgactctgaa
     1621 tggatgggac cgttgtatca atttcatgga ttaacggtag attgtattga taagcaccgc
     1681 cctaattcgc acgaaagacg caatgcctac aaagcagata ttacgtttgg taccaacaac
     1741 gagtttggtt tcgactattt gcgtgataac atggcggggc atccagatga attggtgcaa
     1801 ggaaaacacc actatgccat tgtcgatgag gtcgattctg tattgattga cgatgcacgt
     1861 acgccattga ttatttctgg tccaacacca aaaggtgatg agcatgaatt tcatatttta
     1921 aaaccaagaa ttgaaaacat tgttgcagca caacgtcagt tggttaacca atgtttagca
     1981 gacgcaaaac gtatgttgac cgttattcaa gatggttttg aaaacaaaaa agacggtaaa
     2041 gaaatgttgg agcaaggtgg aattgctttg ttgcgcgccc acagaggttt acctaaaaat
     2101 aaagcgttaa ttaagttctt gtcagagcca ggaattaaag cacatttaca aaaaactgag
     2161 aatttctaca tgcaagaaca aggaaaacgc atgaaagaaa ttgacaaaga attgttcttc
     2221 gtaattgaag aaaaacaaaa taccattgaa ttaaccgata aaggaatcga cttaatttcg
     2281 gggcaagacg atagaaactt cttcgttatt cctgatattg ggttgcaaat tgctgcaatt
     2341 tcaaaagaaa atttaccgca agagcaattc caagctaaaa aagcagaaat tttaagagaa
     2401 tatagcatta aatctgagcg tattcactcc atcaatcaat tgttgaaagc atacacgttg
     2461 tttgaaaagg aggtagaata tgtggtgatg gatggaaaaa ttaaaatcgt tgacgaatct
     2521 acaggtcgta tcatggaagg ccgtagatat tctgacggat tgcaccaagc cattgaagct
     2581 aaagaaaatg taacagtaga ggcagcaaca caaacgtatg caaccattac tttgcaaaat
     2641 tacttccgta tgtaccacaa attggcgggt atgaccggta cggcagaaac agaagcaaaa
     2701 gaattctggg atatttacaa attagacgtt gtggttattc ctaccaacaa acctattttg
     2761 cgtaaagacc aaaacgactt ggtattcaaa acagcacgtg aaaaatttgc tgctgttatt
     2821 gaagaaacag aacgtttggt ggcgcaagga agaccagtat tggtgggtac aacaaccgtt
     2881 gaaatttctg aattattgag ccgtatgctt aaaatgaaag gaattaaaca caacgtgttg
     2941 aacgccaaac agcaccaaaa agaagcagaa attgttgccg aagcaggtat tgctggagca
     3001 gtaaccattg caacaaacat ggctggtcgt ggtaccgaca tcaaattggg cgaaggcgta
     3061 aaagaagctg gaggtttggc aattgttgca acagaaagac atgattctcg ccgtgtggat
     3121 agacagttga gaggtcgttc tggacgtcaa ggagacccag gttcttctca attctttgtt
     3181 tcgttggaag acgatttgat gcgtaaattt ggttcagagc gtattgctaa attaatggac
     3241 cgcatgggct tgaaagaggg cgaagttatc acccactcaa tggtttctaa atccattgaa
     3301 agagcacaga aaaaagtaga agaaaacaac tttggtattc gtaaacgttt gttggaatat
     3361 gacgacgtga tgaatgcaca acgtaaagca gtttacaaaa aacgtagaaa tgccttgttt
     3421 ggtgaacgtt tggatgttga tattgacaac atgttgtacg atacagctga ggcaataaca
     3481 atgaaaaact ggaaaggtga ttttgcagtg tttgaagacg aattgattcg tgatttgaac
     3541 attcaatcgc ctgtaactcc agaagaattt gcaactattg atcaagttcc gttgacagaa
     3601 aaagtgtaca tggcaatgat tcgccaatac aacgagaaaa acgaacgcat catcaattta
     3661 tgctggccac aaattaaata cgtgcaagaa aatgaatcgc accgttataa aacaatggca
     3721 attccgttaa cagatggaag acgcacaatg caaattggtg ttcaattaga agaagcctac
     3781 aaagcagagg gtaaaaacat ttctaaacaa ttagaaaaag caattgtatt aaacatcatt
     3841 gacaacgaat ggaaagagca tttgcgtgaa atggatgaat tgcgttcggc ggtgaacaat
     3901 gcacaatacg aacaaaaaga tccattattg gtttacaaat tggaatcgtt tgaattgttc
     3961 aaaaacatgc atgctcgctt aaataaagaa gctgttgaat tgttgatgaa actggacatt
     4021 gaagtggata acaaagtaga agcttcaacg caagaaattc gtcaagaaaa ccactatgca
     4081 aaagctaagg aaacagtttc ttcggatatt caaaacacgc gcaacgatta tcaagaagca
     4141 atggaaagtt cgcaccaaca gccggtaaaa agagaacctg ttgttgtgga agacaaaatt
     4201 ggtagaaacg atccttgtcc ttgcggtagc ggaaaaaaat acaaacaatg tcacggtaaa
     4261 taaacaaccc aacattgaat aaaaagaata gaaatcagtg taattcgaaa agagttacac
     4321 tgattttttt tggtaccttt gcactataaa attttacatt gatacaaaca gttggcataa
     4381 ttggttctgg aaacgtggcg tggcatttag cacatgcgtt gcaagcattg ccaaccgtta
     4441 aattgacttg gatttatagc cgaaatgcac aaacattggc tgagctcgga acagaattaa
     4501 acgttttcac ctccaccact ctaccatctt cagcagttga tttgatattg gtgtgtgtaa
     4561 acgacgattc cattcaaaca attgtcagcc aattgccaaa agattcaaat attgcatata
     4621 cgtctggcgt tgttgaacta aacagcattg tatttactca aaaaaactac ggtgtttttt
     4681 atccattgca aacatttacc cgaaaaaagg aaattgcatt aaacgaagtt ccgtttttaa
     4741 ttgaagcaag caacgaagca tttacttgtg aattggaaca attggcagta ttgctaagta
     4801 aaaacgtg
//
LOCUS       DAOQVQ010000139         4770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_19850_length_4770_cov_3.832025, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000139 DAOQVQ010000000
VERSION     DAOQVQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19850_length_4770_cov_3.832025"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="PKN22_05500"
     CDS             complement(<1..1791)
                     /locus_tag="PKN22_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42195.1"
                     /translation="MIDHQAFDQWKTLKSEQISKSGNYVTYEIQPLKGDGYLYWYNTK
                     ENRLDSVIRGTKAQFSSDEKYLIYTLEPGYDTLRKVELNKVDKKKWPKDSLAIYSFST
                     KKTEKIANIKSFQLNEKHAWLGYLSNDNKLKDTSTAKKKKSKKKTPEKKEPKSEGTVL
                     TVRNLENNKNYTFKNVTSYQFSEKGELLALVQNQNDTTILSVVDLNEGKIDIVQNFFK
                     AIEGVTFDKQLSRCLFLASSDTTKTKIFKLYNYTLSNCELNVLIDTTSQQMPKGITVS
                     QYKTPEFSKYNNGFYFGAYAVPQPEKKDTLLESEKVKLDIWTYNEPELHTQQLNNLQK
                     DLKKAYWFYYDLSTNKVVQLENDTLDLQADFKDLQQYALVSNSRPYALSSMWDISGKR
                     DYYRINLQTGATQLIKKGVANSIRLSRKGDLFVYFQSEDANYYVVDLEKNTADCITCS
                     EKNRTWTNDKNGMPMDDEPLGNLGWNKAGTHLFLKEKNDFFAYDFSNNTLINLTNNEG
                     VKHNIEFDAARFQTDSNYVELDEIYLLGIEKSTKSAHFYTFKEDKMQKQGVWDAKITS
                     VKKAKDNTSMIIRKMNVHDYPEVFLSDNSSN"
     gene            2101..2970
                     /locus_tag="PKN22_05505"
     CDS             2101..2970
                     /locus_tag="PKN22_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS42196.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEISKVEMISPEEEVELAKRIK
                     DGDQAALERLTKANLRFVISVSKQYQNQGLSLPDLINEGNMGLIKAAERFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIVRLPLNKVGNINRINRAFSELEQKYERPPTIEELAE
                     HLDYTVNDVRQSMINSGRHVSMDAQLVEGDESSSSLYDLLTNDALPAPDDSLKVESLR
                     KDIERSLSTLTSREGDVVRLFYGLNGKAPLSLEEIGDRFDLTRERVRQIQEKAIRRLR
                     HTARSKMLKTYLG"
     gene            complement(2967..>4770)
                     /gene="priA"
                     /locus_tag="PKN22_05510"
     CDS             complement(2967..>4770)
                     /gene="priA"
                     /locus_tag="PKN22_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNS42197.1"
                     /translation="LLLLKLQADNKEAYIPKKKVLEEGMSASTLNTLEKNGYIVIEKL
                     AVSRLKSIDSTDKAIKELSEDQQKAFLQIKQSFESHFVTLLHGVTGSGKTEIYMKLIK
                     EQLEAGKQVLFLLPEIALTTQLIQRLSIYFGELIGVYHSKFNQNERVEIWNNVLENNP
                     TKFRLIVGARSSIFLPFNNLGLIIVDEEHESSFKQYDPSPRYNARDAALVLAKIHHAK
                     VLLGSATPAMESYYNAQTGKYGLVELNKRFSGVQLPEILCADMRKARRQKSVQSDFSL
                     FLLDEIKETLKNNKQVILFQNRRGYTPMWMCEICNWTPKCVNCDVSLTYHKHTNSLKC
                     HYCGHLQAPMGTCSACGSTRIKMLGFGTEKIEDDLQIILPNAVIQRLDLDTTRSKTAY
                     ETILNDFGEGKINVLIGTQMVSKGLDFKNVALVGILDADMMLNRADFRAFERSFQLMS
                     QVAGRAGRDKERGKVIIQTGDPEHWVIQKVHQHDYIGFYKHELIERKNFFYPPYYKII
                     YILLKHKDEHVLNRGAEEFSRALRAVFKERVLGPEFPSIKRIQNFYLKQIILKIERDA
                     PDAQVKDKISELLDNFLSHADNKSIKVVLDIDPA"
BASE COUNT         1455 a          909 c          808 g         1598 t
ORIGIN      
        1 gttggaagaa ttatcactta aaaacacctc tggataatca tgcacattca ttttacggat
       61 aatcattgaa gtgttgtctt ttgctttttt tacactggta attttagcat cccaaacacc
      121 ttgtttttgc attttatcct ccttgaaagt gtagaaatgt gcacttttag tagatttttc
      181 tatacccaaa agatagattt cgtccaactc cacataatta gaatctgttt gaaaacgagc
      241 tgcatcaaac tcaatgttat gcttcacacc ttcgttattt gtcagattaa tcaacgtgtt
      301 atttgaaaaa tcatatgcaa aaaaatcgtt tttctctttt aagaataaat gcgttccagc
      361 tttgttccaa cccaaatttc ccaatggctc atcgtccata ggcattccgt ttttatcatt
      421 tgtccacgta cgattttttt cagaacaggt tatacaatca gctgtattct tctctaaatc
      481 tacaacataa taattagcat cttcactttg gaaataaaca aacaaatcgc cttttcgtga
      541 taaacgaatt gagttggcaa cccctttttt aatgagttgt gtggcacctg tttgcaagtt
      601 aatacggtaa taatcgcgtt ttcctgatat atcccacatg gaacttaacg catacgggcg
      661 actgttactc accaaagcat attgttgcaa atctttaaaa tcagcttgta aatctagtgt
      721 atcattttcc aactgcacca ctttattggt tgacaaatcg taataaaacc aataggcttt
      781 tttcaaatct ttttgcaaat tatttaattg ttgtgtatgc aattcaggtt cgttataggt
      841 ccaaatatcc aattttactt tctcactttc caaaagcgtg tctttctttt cgggttgcgg
      901 aacagcataa gcaccaaaat aaaaaccgtt gttgtattta gaaaattcgg gcgttttata
      961 ctgcgaaact gtaattcctt taggcatttg ctgcgatgtg gtatcaataa gaacgtttaa
     1021 ctcacaattg gaaagtgtat agttgtataa tttaaaaatt ttggttttgg tggtatccga
     1081 cgatgctaaa aacaaacatc tggataattg tttatcaaat gtaacacctt caattgcctt
     1141 aaaaaagttt tgaacgatat caattttacc ttcgtttaaa tcaaccactg ataaaatggt
     1201 cgtatcattt tgattttgaa ccagagccaa caactcgcct ttttctgaaa attggtaact
     1261 ggtaacattt ttaaacgtat agtttttatt gttttccaaa tttctgaccg ttaacaccgt
     1321 tccctccgat ttaggttctt tcttttcggg tgtttttttc ttactttttt tctttttagc
     1381 agtcgaagtg tcttttaact tattatcgtt actcaaataa ccaagccatg catgtttttc
     1441 attcaactga aaacttttaa tgttggcaat tttttctgtt tttttagttg aaaatgaata
     1501 aatagcaagt gaatcttttg gccatttttt cttatctact ttgtttaatt ctacttttct
     1561 caacgtgtca tagccgggct ctagggtgta aatcaaatat ttttcatccg aactaaattg
     1621 cgcttttgtt cctctaatta cagaatccaa acgattttct ttggtgttgt accaatacaa
     1681 atatccatca ccttttaaag gttgaatttc ataggtaaca taattgccac tttttgaaat
     1741 ctgttcactt ttcaaggttt tccattggtc aaaagcttga tgatcaatca cttttttttg
     1801 tccccaaaca gatgagatag aaaataaaca gaatacaaca aacagaacaa agaagctatt
     1861 tttcataaca aaatgtaaaa aatgaattaa aataatgtat aaaagtaata aattctaaca
     1921 aatagaaaat aacaaagcaa ctaactgtga ctttcatata cattagtgca acttcaacta
     1981 tgttaaaata tcaacaaaca aatttattta taaaattatt gatttttagt tgtttacaag
     2041 caacttaatt actaacttaa gcgtattatt attgaaagac ttctaaaaaa ccacttaact
     2101 atgagacaac ttaagattac aaaacaggta accaaccgtg aaaccgcctc gttggacaag
     2161 tatttgcaag aaattagcaa agtagaaatg atttcacccg aagaagaggt ggaactggca
     2221 aaacgtatta aagatggcga tcaagcggca ttagaacgtt tgaccaaagc taatttacgt
     2281 tttgttattt cggtatccaa gcagtatcaa aaccagggat tatctttacc cgaccttatc
     2341 aatgaaggca acatgggctt aataaaagct gccgaacgat ttgatgaaac gcgtggattc
     2401 aaatttattt cctatgccgt atggtggatt cgtcaatcca ttttgcaagc attggcagaa
     2461 caagctagaa tagtgcgttt accgcttaat aaagtgggaa atattaaccg catcaaccgt
     2521 gctttttcag aattggaaca aaagtatgaa cgcccaccaa caattgagga attggctgaa
     2581 catttagact atacggttaa cgatgtgcgt caatctatga ttaactcagg ccgccacgtg
     2641 tcgatggatg cgcaattggt agaaggcgat gaatcttcgt ctagtttgta tgatttattg
     2701 acaaacgatg cattgccagc tcctgacgac tcgctaaaag tagagtcatt gcgcaaggac
     2761 attgaacgct cgctttcaac actaacttcg cgtgaggggg atgtggtgcg tttgttttat
     2821 ggcttgaacg gaaaagctcc tttatcgttg gaagaaattg gcgatcgttt tgatttaact
     2881 cgtgaacgtg tacgtcaaat tcaggaaaaa gctattcgtc gtttacgcca tacagcacgt
     2941 agcaaaatgc taaaaactta tttgggctaa gcaggatcta tgtccagcac aactttgatg
     3001 gatttattat cggcatgact taaaaagtta tccaataact cgctgatttt atctttaact
     3061 tgagcgtctg gtgcatcgcg ttctatcttc aagataattt gcttgaggta aaaattctgt
     3121 atgcgcttga tggatggaaa ctcaggtccc aacacacgct ctttaaacac agctcgcaag
     3181 gctcgtgaaa attcttctgc tcccctattg agcacatgtt catctttgtg tttcaacaag
     3241 atgtaaataa tcttgtaata aggtgggtag aagaaatttt tgcgttcaat gagttcatgc
     3301 ttataaaaac ctatgtaatc gtgttgatgc actttttgaa tgacccaatg ttcgggatcg
     3361 cctgtttgga tgataacctt cccacgttct ttatcgcgac cagcacgacc tgccacttgt
     3421 gacataagtt ggaaactacg ttcaaatgct ctaaaatcgg cacggttcaa catcatatcg
     3481 gcatctagaa tcccaaccaa agcaacattc ttaaaatcca acccttttga aaccatttgt
     3541 gttccaatca acacattgat ttttccttca ccaaaatcgt tcaatatggt ttcataagct
     3601 gttttgcttc gggtagtgtc taaatccaaa cgttgaatga cggcattggg caaaataatt
     3661 tgcaagtcgt cttcaatttt ttctgttcca aatcccaaca tttttatgcg tgtgcttcca
     3721 catgcgctac acgttcccat gggagcttgc aagtgaccgc aataatggca ttttaacgaa
     3781 ttggtgtgtt tgtgataggt caacgaaaca tcgcagttaa cacatttagg cgtccaatta
     3841 catatttcac acatccacat aggggtgtaa ccgcgtctat tttgaaacaa gatgacttgc
     3901 ttgttgttct ttaacgtttc tttgatttca tctaataaaa atagtgaaaa atcagattgc
     3961 acactctttt gtctgcgcgc ttttcgcata tcggcacaaa gaatttcggg caattgaaca
     4021 ccactaaaac gcttgttgag ctctactaag ccatatttgc ctgtttgtgc attgtaatac
     4081 gattccatgg cgggtgttgc cgaacctaac aaaacttttg catggtgaat ttttgccaac
     4141 accaacgccg catcacgggc attgtatcga ggtgatggat cgtattgttt gaatgaactt
     4201 tcgtgttctt catcaacaat aatcaaccct aaattgttga acggcaagaa aatagatgaa
     4261 cgtgcaccta caattaatcg aaatttagta gggttgtttt ctaaaacatt gttccatatt
     4321 tcaacgcgtt cattttgatt gaacttagaa tgataaactc caattaactc cccaaaataa
     4381 atggaaagtc gctgaattaa ttgtgtagtt agcgcaattt cgggcaataa aaacaacacc
     4441 tgcttacctg cttccaattg ttctttaatg agtttcatgt agatttctgt tttaccagaa
     4501 cctgtaactc catgcaacaa cgtaacaaaa tgactttcaa acgattgctt aatttgcaag
     4561 aaggcctttt gctgatcttc tgacaactct ttgatggctt tatcggttga atcaattgat
     4621 ttaaggcgac taacagccaa tttttcaata acgatgtagc cgtttttttc caacgtattc
     4681 aacgtagagg ccgacattcc ttcttccaat acttttttct ttggaatata ggcttcttta
     4741 ttatcggctt gcaattttaa gagcaataac
//
LOCUS       DAOQVQ010000140         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20314_length_4692_cov_4.034721, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000140 DAOQVQ010000000
VERSION     DAOQVQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20314_length_4692_cov_4.034721"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PKN22_05515"
     CDS             <1..129
                     /locus_tag="PKN22_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNS42198.1"
                     /translation="YTKKFPAGEFQVPCALIIGKRKASTDLKTSLNDALREHNVAV"
     gene            complement(146..469)
                     /locus_tag="PKN22_05520"
     CDS             complement(146..469)
                     /locus_tag="PKN22_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42199.1"
                     /translation="MYIHVSSEVEESVPIAMKMVEKLSEMTNRKKVPLLFNFDEFALP
                     GKANREFWSKKEACPYSLADAFIVNSDAKRIIANFYLKINKPERPTKMFPDEESALEW
                     LKTFL"
     gene            668..3214
                     /locus_tag="PKN22_05525"
     CDS             668..3214
                     /locus_tag="PKN22_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS42200.1"
                     /translation="MINKIKRFKFEYKITALMLLVLIVVLATGTAAYFRYSFLLNNIN
                     QSMQPDAQLNKIQMIRNELAELSNIAKTHSLTGDDVYYEAFSFKREKIMKELASSDSK
                     KNAEHILLDTLITNRLIVLDGIMFAEDPYRVQTALWKVLKNIENYDNTSTNNQLKIMQ
                     LELKKNNESLEQINFEYLTENKKRLEEIKQEELKLQQKLERANKRNNGKKSKALDSLI
                     QLKRKEAKILERNLSKEVNKNAHLSIEKIYSGIENVSQEELTIENEIKSNQLKLISMD
                     NLLNARISAIVDNLELKERNKLAKATHYATSESKKTAAVVGVFSAFVAVLIFLISYFI
                     KSYVQKNRQYEKALRLSASEKQRLISTRERLVSSISHEIRTPMHAIAGFAEQLSKHNL
                     RTDQQEFVTLIQKSSKHLTYLVNDILDLSKLNNDKLKLNYYPFCFNGLVNEITDYTSS
                     LQLGKDVKVNIEIDPQSSNFYMGDEYRLRQILINLVGNAIKYTEVGSVTLKVNVVKSE
                     NSKDTLQFIVQDTGVGMSKDALDKAFSEFEQFSEVRTSNEVGTGLGLAITKKIVELFN
                     GSILINSELGKGTTVQVEIVLERTFKPQKKMTEVNNIPCSSVLIVDDEVYNRKLVNAM
                     LSPFLLEINEVENGKEALDILLSKPIDLILLDAKMPVMDGLETIRAIRNLEDKQKSKV
                     KVILLTAADSEISDIIDKVDGFLPKPFNEEQLIDKIVRMFDTDKKEKSQMVNNVVDFS
                     NLKSLSGSDKNFYIDMLQTFVVSTTNSQQVIKAAFAKNDWELLANEAHKIASPCRHIG
                     ANNLHKILKEIEQSARNSQQTFHLKETIKALNKEIELVLDAINTEISTLS"
     gene            3225..4589
                     /locus_tag="PKN22_05530"
     CDS             3225..4589
                     /locus_tag="PKN22_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS42201.1"
                     /translation="MNNNDFLIYVVEDNEWYNKLLVHTISLNPDFEVKSFFSGEEMLK
                     NLTPKVKVITLDYRLGDTTGDQLLPLIKKVSPSTEVIVVSEQSDISVAIELLKLGAYD
                     YIVKSEDIKSRILNSINHIKGNMGLKEQIQLLQTEVEKKYDFQNTITGQCEPIKNVHR
                     MVSKAINNTITVCITGETGTGKEVVAKAIHYNSDRKNKPFVPINMAAIPEDLIESELF
                     GNEKGAFTGAQNRRIGKFEQANGGTLFLDEIGEMDIHFQAKLLRALQEREITRIGGNE
                     LIKIDCRIIVATNRDLLQEVKAGNFREDLYYRLFGLPIHLPPLRERGNDVLMLAKQFI
                     GDFCKENNFAPKKISDDARSKLMAYSWPGNIRELKSVVELSVVMSDTNEIHASDLTIQ
                     PQEFLSEILQRDLTMREYEIAIVKHFMKRFNDSTKMVADKLEIGQTTVYRLLKEAEES
                     STTN"
BASE COUNT         1724 a          670 c          877 g         1421 t
ORIGIN      
        1 tataccaaaa aatttccagc aggtgaattt caagtgccgt gtgcattaat tatcggtaag
       61 cgaaaagcct ctacagattt aaaaacttca ttaaacgatg cgttgagaga gcataatgtt
      121 gctgtttaat gtgtaatttg taaggttata aaaacgtttt caaccattcc aaagcacttt
      181 cttcgtctgg aaacattttg gttggtcgtt cgggtttatt tattttaaga taaaagttgg
      241 caataatacg ttttgcgtct gagttaacaa taaaagcatc ggctaaagag taggggcaag
      301 cttctttttt agaccaaaat tcacggttgg cttttcctgg taatgcaaat tcatcaaaat
      361 taaaaagaag aggaactttt ttacggttgg tcatttcact cagtttttca accattttca
      421 ttgcaattgg aacactttct tccacttccg aagatacgtg aatgtacata ataccatcac
      481 ttttataaga tagataataa ggtgcaacag catcttggac tattatattt gaatggagtg
      541 tttgcataaa tgtctaaaag tctttcttgt aagtagctga aacaaatata aaaaagtttg
      601 gttagataaa aaaaaatagt ttttattctt gattaatttt ataattttag ttgttaaaca
      661 gaggtggatg ataaacaaaa taaagcgatt caaatttgaa tataaaataa cggcactaat
      721 gcttttggtg ttgattgttg tgttggctac tggtactgca gcctattttc gctactcttt
      781 tttgctaaat aacatcaacc agtcgatgca gccagatgca caactgaata aaattcaaat
      841 gattagaaat gaattggctg agttgtcaaa tattgccaaa acgcatagtt taactggaga
      901 tgatgtttat tatgaagcat tttcgtttaa acgcgaaaaa ataatgaaag aattagcctc
      961 atccgattct aaaaagaatg cagaacatat tttattggat actttaatca ctaatcggtt
     1021 aattgtgttg gatggaatta tgtttgcaga agacccttat cgtgtacaaa ctgcattgtg
     1081 gaaagtattg aaaaatattg aaaattatga caatacaagc actaataatc aattaaagat
     1141 tatgcaactt gaattgaaaa aaaataatga atcgttggag caaattaatt ttgaatattt
     1201 aaccgagaat aaaaaaaggt tagaagaaat taaacaagaa gaattaaaat tgcagcaaaa
     1261 gctagaacgt gctaataaaa gaaacaatgg caaaaaatcg aaagcattag actcactaat
     1321 ccaactcaag cgaaaagaag ctaaaatatt ggaacgcaac ctatcaaaag aggtgaataa
     1381 aaatgctcat ctttcaattg aaaaaattta ttcggggata gaaaatgtaa gtcaagaaga
     1441 gttaaccatt gaaaatgaaa taaaatccaa tcagttgaag cttatttcta tggataattt
     1501 gttaaatgca cgcatttcag ctattgtgga taatttagag ctaaaggaaa gaaataaatt
     1561 ggctaaagca acacattacg ctacctctga aagtaaaaaa acagcagccg ttgtgggtgt
     1621 ttttagtgca tttgttgctg ttttaatctt tttaattagt tattttataa agagttatgt
     1681 gcaaaaaaat cgccagtatg aaaaggcatt acggctttcg gcaagtgaaa aacaacgatt
     1741 gatttcaaca agagaacggt tggtttcttc tatcagccat gaaattagaa caccaatgca
     1801 cgccattgcg ggatttgctg aacaattgtc taaacacaat ttacgcaccg accaacaaga
     1861 atttgtgacg ttgattcaaa aatcatccaa acatttaacg tatttggtca atgatatttt
     1921 ggacctatcc aaattaaaca atgataaact aaaattgaat tattatccat tttgttttaa
     1981 cggcttggta aatgaaataa ccgattatac cagtagtttg caattaggaa aagatgttaa
     2041 ggttaatatt gaaattgatc cccaatcatc caatttttat atgggagatg aataccgatt
     2101 aaggcaaata ttaatcaact tagttggaaa tgcaattaaa tatacagaag ttggttcggt
     2161 tactttaaaa gttaacgtag ttaaatctga aaattctaaa gataccttgc aatttattgt
     2221 gcaagacaca ggtgtaggaa tgtcgaaaga tgcattggat aaagcttttt cagagtttga
     2281 acagtttagt gaagtacgaa catctaatga agtgggaaca ggtttagggc ttgccataac
     2341 taagaaaatt gttgaacttt tcaatggctc aattctcatc aacagtgaat taggaaaagg
     2401 tacaacagtg caagtggaaa ttgtgttaga acgcaccttt aagcctcaaa aaaagatgac
     2461 agaagtaaat aacatacctt gttcatcggt gttgattgta gatgatgaag tttacaaccg
     2521 aaaattggtg aatgcaatgc tatctccctt tttattagaa atcaatgagg ttgaaaatgg
     2581 gaaagaagca ttggatattt tattgtctaa accgatagat ttaattttgt tggacgctaa
     2641 aatgcctgtt atggatggac tggaaaccat acgtgcaata agaaatttag aagacaagca
     2701 aaaaagtaag gtaaaagtaa ttttgttaac agcggcagac agtgaaataa gtgatattat
     2761 tgacaaagtt gacggatttc tacccaaacc atttaatgaa gaacagttaa ttgataaaat
     2821 cgttcgtatg tttgatacag ataaaaaaga aaaatcccaa atggttaata atgttgtaga
     2881 tttctcgaat ttaaagtcac taagtgggtc ggataaaaac ttctacatcg atatgctaca
     2941 aacatttgta gtttctacaa ccaattcaca acaagttatt aaagcggctt ttgcaaaaaa
     3001 tgattgggaa ttattggcca atgaagcaca taaaattgct tctccttgtc gacatattgg
     3061 agccaataac ttgcataaaa tcctcaaaga aatagagcaa agtgcaagaa atagtcaaca
     3121 aacttttcat ttaaaagaaa cgataaaagc attaaataaa gaaattgaat tggtgttaga
     3181 tgccattaat actgaaattt caacattatc ctaatttgta atcaatgaac aataacgatt
     3241 ttctgatata tgtggtagaa gataatgaat ggtacaataa attacttgtg catacaattt
     3301 cattaaatcc cgattttgaa gtgaaatctt ttttctctgg tgaagaaatg ttgaaaaacc
     3361 ttactccaaa agtgaaagtg attactttgg attatcgttt gggtgacaca accggcgatc
     3421 aattattgcc attgattaaa aaggtgagcc catctaccga agtaattgtt gtttctgaac
     3481 aaagtgatat ttctgtggcc attgaattgt tgaaattggg ggcgtatgat tacattgtta
     3541 aatcggaaga tataaaaagt agaattctga attctatcaa ccatattaaa ggaaatatgg
     3601 gattgaaaga gcaaattcag ctacttcaaa cagaagttga aaaaaaatac gattttcaaa
     3661 ataccataac cggacaatgt gaacccatca aaaatgttca tcgcatggtg agcaaagcca
     3721 tcaacaatac aataacagtt tgcataactg gtgaaactgg tacgggtaaa gaagtggttg
     3781 ccaaagcgat acactacaat tctgatcgca aaaacaagcc ttttgttccc atcaacatgg
     3841 ctgccatccc tgaagatttg attgaaagtg aattgtttgg aaatgaaaaa ggtgctttta
     3901 caggtgctca aaatagacgc ataggaaagt ttgaacaagc caatggtggt actttgtttt
     3961 tggatgaaat aggggagatg gacattcatt ttcaagcaaa attactcaga gcattgcaag
     4021 aaagagaaat tacacgtatc ggtggaaatg aacttattaa aattgattgc cgcataattg
     4081 tagcaaccaa cagggatttg cttcaagaag taaaagctgg aaattttaga gaagatttgt
     4141 attatcgttt gtttggttta ccaattcatt tgccgccatt gcgtgaacgt ggcaacgatg
     4201 tgttgatgtt ggcaaagcaa tttattggtg acttttgtaa ggaaaataac tttgcaccta
     4261 aaaaaataag tgatgatgca cgttcaaaat taatggctta ttcgtggcct ggaaatattc
     4321 gagagctgaa atcggttgtt gaattaagtg tggtaatgtc ggataccaat gagattcatg
     4381 caagtgattt aacaattcaa ccgcaagagt ttttatccga aatacttcaa cgtgatttaa
     4441 cgatgcgaga atacgaaatt gcaattgtca aacattttat gaaacgtttc aatgatagta
     4501 caaaaatggt ggctgataaa ttagaaattg gtcaaacaac ggtttatcga ttactcaaag
     4561 aagcagaaga aagctccact accaattaaa tagaatcaag cacaaattgt gtcatttcag
     4621 cgctggttcc atacggaatc caaatggcat ctgggtttct tttaaaccaa gtaacttgtc
     4681 gtttggcata tc
//
LOCUS       DAOQVQ010000141         4689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20338_length_4689_cov_3.733492, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000141 DAOQVQ010000000
VERSION     DAOQVQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20338_length_4689_cov_3.733492"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKN22_05535"
     CDS             complement(<1..725)
                     /locus_tag="PKN22_05535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNS42202.1"
                     /translation="MPSVILLSFCESPSSDIIQTLQIVYGKNITFVVMMMPWIAIFFS
                     IIVASRYILKWSFSYLFTFRSKIDGKRFLFGFIVWFCISMVTFYTSLNENVVFNFKWQ
                     SFLPLILVATITLFIQCTAEELVFRSFIYRWIGTSIPKGFVQVIVSGVLFGLLHGSNP
                     EVATLGNKALLYYIGTGIFLGLLVLLDDGIELSTGFHFANNLFAALIVSKNWQVFQTD
                     ALFIDNNPPSFTRIDSIFTFSEQA"
     gene            complement(824..1747)
                     /locus_tag="PKN22_05540"
     CDS             complement(824..1747)
                     /locus_tag="PKN22_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS42203.1"
                     /translation="MEPIIQPIDRNVLKAELSADKFVRITRKGDNEIYIINHHNAPNV
                     VKEIGRLREVTFRRSGGGTGLSIDLDEFDTNEHCYEQLIVWSPEDEEIIGGYRFILCK
                     YAILENEIALSTSHYFHFSDDFVKNYLPHTIELGRSWVQPNYQPNINYRKGLFAMDNI
                     WDGLGALIIKYPEIKYFFGKVTMYPTYERDARDFLLLFLNHFFPDTEALVKPIHPLIV
                     GSNKPFEELKKQELPYKESYRILNAYVRDKGEVIPPLVNIYMNLSSTMKTFGTAVNPD
                     FGNVEETGILVTINDIFPEKKERHMTGDINN"
     gene            complement(1752..2585)
                     /locus_tag="PKN22_05545"
     CDS             complement(1752..2585)
                     /locus_tag="PKN22_05545"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNS42204.1"
                     /translation="MKQQKFIDIQKLVASKSPKLAKFIPGFVYSYLKRILHQDEINAF
                     MAKNEHLENEEFCSAVITQFNIQVEGKNLESIPNSGGVILVMNHPLGGMDAMALVHLL
                     KNKRRDIKFIVNDLLLNLTPIKGLFVGVNKHGANSRKLPEQMEELFSSEQLIGIFPAG
                     LVSRKKKGKVEDLEWKKTFVKYARNHHKTMIPIYIEGRLSNFFYNLSNIRSFLGIKTN
                     LEMLYLSDEMHNQKGKTIRFVVGEPFTIDSFEELKSDKKIAEAIKNKVYRLKSQLDNK
                     N"
     gene            2745..4325
                     /locus_tag="PKN22_05550"
     CDS             2745..4325
                     /locus_tag="PKN22_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNS42205.1"
                     /translation="MKKLFLFSLFGNLLFFNHAQVRNNKLNEAYMYVNRFYVDKVDDN
                     KLQEAAIVAMLEELDPHSVYISKEEVDDANQSIVGSFVGIGVRFQVFKDTFTVMQTIP
                     GGPSEKVGLQSGDKILSVDGEKIAGVGMKNSQIRNKLMGELNTKVKVEIKRKGEKEPL
                     NFVITRDKIPLHSVDAAYMIDKTIGYIKLNSFSRTTTQEVEDALSKLKEQGMKDLILD
                     LQNNGGGLLTAAHHLADQFLSGDKMIVYSEGRAQPRQEFKAGEKSGFEKGRLIVLTNE
                     NSASASEIVSGAIQDWDRGLIVGRRTFGKGLVQRPIDLSDGSQIRLTIARYYTPSGRF
                     IQKPYENTADYKKDLVNRYDHGEYYHQDSIKLNDSLMVKTLITKRNVYGGGGIMPDIF
                     VPYDTSGISPLFTSLVRKGAINTYLAQYVNNNRASLKSNYPTFDDYSKNFVLTQEFMD
                     AFFKNAKDEYEIEFNEEQYKESEQWLKLNMKALIAQNLFDIPQFYQVMNEKNEVLLRA
                     LEVLRTDEYKKAGLEKSK"
     gene            4435..>4689
                     /locus_tag="PKN22_05555"
     CDS             4435..>4689
                     /locus_tag="PKN22_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42206.1"
                     /translation="MSASAEKYSKRGVTTSYVSTVIGISLVLFVISIIVGGSFALKSI
                     QKQAKESLQADIFFKPEQSDADIKQMEQELRTWKYFKEIKF"
BASE COUNT         1573 a          764 c          809 g         1543 t
ORIGIN      
        1 gcctgctcac taaaggtaaa aatgctatca attctagtaa atgaaggagg attgttgtct
       61 ataaatagag catctgtttg aaaaacctgc cagtttttgc ttacaataag tgctgcaaac
      121 aagttattgg caaaatgaaa gcctgtagaa agttcaattc catcatccaa taatacaagt
      181 aaacctaaaa aaatacctgt acctatataa tatagaagtg ctttgttgcc taatgttgct
      241 acttctggat ttgaaccgtg taataatcca aataacactc ctgaaacaat tacttgcacg
      301 aaaccttttg gaatagatgt tccaatccac ctatatataa aggaacggaa aactaattct
      361 tcggcagtac actgaataaa caaggtgatt gttgctacta atattaaggg taaaaagctt
      421 tgccatttaa aattgaaaac tacattttca ttaagtgatg tataaaaagt aaccatacta
      481 atacaaaacc aaacgatgaa tccaaaaaga aatcgtttgc catcaatttt acttctaaat
      541 gtaaataaat acgaaaaaga ccattttaat atatatcttg aagcaactat tattgagaaa
      601 aagattgcaa tccatggcat catcataaca acaaaagtta tgttctttcc atatactatt
      661 tgaagtgttt gaataatatc acttgatggt gactcgcaaa acgataataa aattactgat
      721 ggcatgcttc caacaacata agctacgcat attattaata gcgtaaacca atatcctaat
      781 tggtttgtag ttgatgttgg cttcagctgc acaaatcaat aaatcaatta ttaatatctc
      841 ctgtcatgtg gcgctctttc ttttctggaa agatatcgtt aatggttacc aaaatacctg
      901 tttcttccac gtttccaaaa tctggattaa cagcagttcc aaatgttttc atggttgatg
      961 ataaattcat gtagatattt accaatggag gaataacttc tcctttatca cgaacatacg
     1021 catttaatat acggtatgat tctttgtatg gcaactcttg ttttttcaat tcttcaaacg
     1081 gcttgttgga tccaactatt aaaggatgta ttggttttac caatgcttct gtatcaggaa
     1141 agaaatgatt taagaacaat aataaaaagt cacgggcatc gcgttcataa gttggataca
     1201 tcgttacttt tccaaagaaa tatttaattt ccggatattt tattattaat gcacctaacc
     1261 cgtcccaaat attatccatt gcaaataatc cttttcggta attgatgttg ggttgataat
     1321 ttggttgcac ccaacttcta cctaattcta tggtgtgagg caagtagttc tttacaaaat
     1381 catctgaaaa atgaaaataa tgactggttg aaagtgctat ttcgttttcc aaaattgcat
     1441 atttacataa aataaaacga taacctccaa tgatttcttc atcttctggc gaccaaacaa
     1501 tgagctgttc gtagcaatgc tcattggtat caaattcatc taaatcgatg cttaaacctg
     1561 tgccccctcc agatcgtcga aaggtaacct ctcgtaaacg accgatttct tttacaacat
     1621 tgggtgcgtt gtgatgattt ataatgtata tttcattatc accttttcgt gtaatgcgca
     1681 caaatttatc tgcagacaat tctgctttaa gcacatttct atcaattggt tgaataattg
     1741 gttccatttt tttaattttt attgtctaac tgacttttta atcgatagac tttatttttt
     1801 atagcttctg ctattttttt atctgacttt aattcttcaa aactatcaat tgtgaagggt
     1861 tctccaacaa caaatcgaat agttttccct ttttgattgt gcatttcatc tgaaagatac
     1921 aacatttcta agtttgtttt gatacctaaa aacgaacgaa tgttggaaag attgtagaag
     1981 aaattagata atcgcccttc aatgtaaata ggaatcattg ttttatggtg atttctggcg
     2041 tattttacaa acgttttttt ccactccaaa tcttcaactt ttcctttctt ttttctactc
     2101 actaagcccg caggaaaaat tccaattaat tgctcggatg agaataattc ttccatttgt
     2161 tctggcaatt ttctactgtt agccccatgt ttatttacac ctacaaacaa tcctttgatt
     2221 ggtgttaaat tcaacaataa atcattgaca atgaatttaa tatctcttcg tttgtttttc
     2281 aaaagatgta ccaacgccat ggcatccatt ccgcctaaag gatgattcat caccaaaata
     2341 acgcctccag aattaggaat gctttctaga ttttttccct caacttgaat gttgaattgg
     2401 gtaatgacag cgctacaaaa ttcttcattt tcaagatgtt cgtttttcgc cataaacgca
     2461 tttatttcat cttggtgtaa aattcttttt aaataagaat atacaaaacc tggaataaac
     2521 ttggctaatt tagggctttt actggccact aatttttgaa tatctatgaa cttttgctgt
     2581 ttcatgtagc tacgaaatta caattttgaa tcgaaataag cgttcaatat gttaggaaga
     2641 tatcacaaat gattcgttaa tttttaataa aaataaatca aaaccattga aattaagtca
     2701 ttagaggcaa gatttttgtt attttcacgc tataaatttt acaaatgaaa aaattatttt
     2761 tattctccct ttttggcaat ctattgtttt tcaaccacgc acaagttaga aacaacaaac
     2821 tcaacgaagc ctacatgtat gttaaccgtt tttatgtgga caaggtggat gataacaaat
     2881 tgcaagaggc tgcaattgtg gcaatgctag aagaattgga tccacactct gtctatattt
     2941 caaaggaaga agtggatgat gctaaccaat ctattgtagg tagttttgtt ggaattggtg
     3001 tgcgttttca agtttttaaa gatacgttta cggtaatgca aaccattcct ggcggtccgt
     3061 ctgaaaaagt aggtttgcaa tcaggcgata aaatcttatc ggttgatggt gaaaaaattg
     3121 caggggttgg tatgaaaaac agccaaattc gcaacaagct aatgggtgaa ttgaacacga
     3181 aagttaaagt ggaaattaag cgtaaaggag aaaaagaacc tttgaatttt gtaatcacac
     3241 gcgacaagat tccattacat tcggtagatg ctgcctacat gatagacaaa acaattggtt
     3301 atattaaact caatagtttt tctagaacaa ccacgcaaga agtggaggat gctttgagta
     3361 aattgaaaga acagggtatg aaagacctca tcttggattt gcaaaataac ggcggtggtt
     3421 tattgacagc agctcatcat ttggccgatc aatttttgtc gggcgataaa atgattgttt
     3481 attctgaagg gagagcacaa ccgcgacaag aatttaaagc aggcgaaaaa agtggatttg
     3541 aaaaaggtcg attaattgtt ttaaccaacg aaaattcggc ttcggcaagt gaaattgttt
     3601 cgggcgcaat tcaagactgg gacagaggtt taattgttgg acgaagaacc tttggaaaag
     3661 ggttggtgca gcgaccaata gatttatcgg atggttcaca aattcgttta accattgctc
     3721 gttactatac tccttctggg cgttttatcc aaaaaccgta tgaaaacaca gctgattata
     3781 aaaaagattt ggtaaaccgc tatgatcatg gggagtatta ccaccaagat tcgattaaac
     3841 ttaacgattc gttgatggtg aaaacattga taaccaaaag aaatgtatat ggtggtggtg
     3901 gaatcatgcc agatattttt gtgccttatg atacgtctgg aatttcaccc ttatttacaa
     3961 gcttggtacg caaaggagca atcaatactt atttggctca atatgttaac aataacaggg
     4021 cttcgttaaa atcaaactac cccacttttg atgattattc taaaaatttt gtgttaacgc
     4081 aagaatttat ggatgctttc ttcaaaaatg caaaagacga gtatgaaatt gaatttaatg
     4141 aagaacagta taaagaaagt gagcaatggt tgaaattgaa catgaaagca ttgattgctc
     4201 aaaacttgtt tgatattcca cagttttatc aagtaatgaa tgaaaagaat gaagtgcttt
     4261 tgcgtgcctt ggaggtgttg agaaccgatg aatataaaaa agcgggacta gaaaaaagta
     4321 aataaaaagt agcttgcaaa cattttgttg tttgtatttt taattggttg caattttttg
     4381 tgcaattcta ttttttattt tcgtatcagc tataatttgt tcaaaaaatt aacgatgagt
     4441 gcaagcgcag aaaagtacag taaaagaggt gtaaccacaa gttatgtttc aacagtgata
     4501 gggatttcct tggtgttatt tgtgatttct ataattgtgg gaggttcttt tgctttaaaa
     4561 tccattcaaa aacaagccaa agaaagtttg caagccgata ttttctttaa acccgagcaa
     4621 tccgatgccg atataaagca aatggagcaa gaattgcgca cctggaaata ttttaaagaa
     4681 ataaaattc
//
LOCUS       DAOQVQ010000142         4671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20471_length_4671_cov_4.862868, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000142 DAOQVQ010000000
VERSION     DAOQVQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20471_length_4671_cov_4.862868"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..205)
                     /locus_tag="PKN22_05560"
     tRNA            complement(131..205)
                     /locus_tag="PKN22_05560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Val,seq:tac)
     gene            complement(295..1578)
                     /locus_tag="PKN22_05565"
     CDS             complement(295..1578)
                     /locus_tag="PKN22_05565"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNS42207.1"
                     /translation="MLKDQALPKNITTFGFNRNKIQLALTYQDTVDWLFQQFPAYQKI
                     GASAYKPGLENVHLLLNHFGNPHHHLKFIHVAGTNGKGSTCSMLASILKEAGQTVGLF
                     TSPHIHDFRERIRVNGEMATEQFVIDQVQAIQSAQLTIQPSFFEISFLLALLYFQQQN
                     CSVCVIETGLGGRLDATNCIQPEISLITNISIEHTQFLGNTIAAIAREKAGIIKANTP
                     VVIGEYHPEAAPVFKEVATAQHAPIYFASEENIEVPINFPLLGNYQHANYRLVHKALD
                     CLQHVFPVSEKAIELGLKNLNRNSGFYGRMQVVEQAPQVIFDVSHNEAGMKATLDYFK
                     DYKHLVVIYGSSADKDLDKIFQLFPKHFTYFFTEFSNERSATIEKLKHYSEKYEISGQ
                     FFQSPQEALRVAKTIVNKEDTILVLGSFFLIADFF"
     gene            complement(1520..2284)
                     /locus_tag="PKN22_05570"
     CDS             complement(1520..2284)
                     /locus_tag="PKN22_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42208.1"
                     /translation="MELVIVSNKQEVTKSRIFTSAIFLILLLLISLITVTTVTDPPVH
                     SELTKEVPVEFIELNFDPTKDQGGSPGGSGKASDEILNKTNPPQQTGVVTTTDPNATA
                     IQSGKSNKTTSEKPSQNTATTTNNTNPIFGGKGGSGGGNDSGSGGVIGNDNGKGGKGT
                     GTGSGDGSGSGKARVRYNEITTSNIYTNSLIKVTLILQVNEEGKVIGTRVVGKETTTN
                     DQSIISKVASACKAQLKYAKGSSITEEYYHIWIQPQ"
     gene            complement(2456..3334)
                     /locus_tag="PKN22_05575"
     CDS             complement(2456..3334)
                     /locus_tag="PKN22_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNS42209.1"
                     /translation="MQLLDGKKTAASIREELALAVKNRKESGKKIPHLAAILVGNDGG
                     SLTYVNNKVKSCEEIGFESTLIHYDDTITEEALLKKINELNEDPNIDGFIVQLPLPKH
                     IDELKVTQAVLPSKDVDGFHPQNLGNMVLNLPGFLPATPAGIIELLKRNNIETAGKHC
                     VVIGRSNIVGSPVSILLARNTNPGNCTVTLTHSRTKNLTEICQQADIIIAAIGKPEFV
                     TAEMVKEGAVVVDVGTTRVEDATRERGWRLCGDVDFKNVAPKCSFITPVPGGVGPMTI
                     ASLMINTLRAVELKGL"
     gene            complement(3402..4121)
                     /gene="rnc"
                     /locus_tag="PKN22_05580"
     CDS             complement(3402..4121)
                     /gene="rnc"
                     /locus_tag="PKN22_05580"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNS42210.1"
                     /translation="MFQFGRIFRFGRNDKQLDLTQFIISRFGYRPKNIALFEKAITHK
                     SNVNTKFAESNERLEFLGDTIIDAVVASLLYDRFPKEDEGYLTKVKSKLVSRKTLSEI
                     AEEMEIRNVLNYHKGRSININTLEGNAFEAIIGAIYLDGGFDAVQKSLKRHIYRKYVN
                     INRILEEEIDFKSRLYIFCQRERFSIDFQVISEVNTGQSWEYKVVVLLNEKKYGLGIG
                     PSKKAAEQAAAKETLELIGVI"
     gene            complement(4124..>4671)
                     /locus_tag="PKN22_05585"
     CDS             complement(4124..>4671)
                     /locus_tag="PKN22_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HNS42211.1"
                     /translation="SGALILEELEHALARGAKIYAEVIGGGMSGDAYHITAPHPEGLG
                     AKNTMLAALEDANISPNEVDYINVHGTSTPLGDIAEVKAIQQVFGEHAYDLNISSTKS
                     MTGHLLGAAGAIEAIASIMAVKEDIVPPTINHFTDDPELDPKLNFTFNKAQKRTVNVA
                     LSNTFGFGGHNTSVIVKKFSK"
BASE COUNT         1361 a          932 c          804 g         1574 t
ORIGIN      
        1 gactgaatac atctttttgt gggaaatgac ggttccgata gctatcggaa cgaaccgccg
       61 accttaacaa ttatttcaca tcgggataaa ctaaatagca aaaaaaaagc catccgaaga
      121 cagctttttg tgggaaatga cggattcgaa ccgccgaccc tctgcttgta aggcagatgc
      181 tctaaaccaa ctgagctaat ttccctttct ttatatttca actaactcgt ttgttaattg
      241 tgggtgcaaa tctaaggtat ttttttcatt ctgcaagcat ttttttagaa aaaattaaaa
      301 aaaatctgca attaaaaaaa agctgcccaa taccaaaatc gtatcttctt tatttacaat
      361 tgtttttgca actcgcaacg cttcttgagg tgattgaaaa aattgtccag atatctcata
      421 tttttctgag tagtgcttca atttttcaat agttgccgaa cgttcatttg aaaattctgt
      481 aaaaaaatag gtaaaatgct tgggaaataa ctgaaaaatc ttgtctaaat ctttatctgc
      541 acttgaacca taaatcacaa caaggtgttt gtaatcctta aaataatcaa gtgttgcctt
      601 catgccggct tcattgtgcg aaacatcaaa tataacttgt ggagcttgct ctaccacttg
      661 cattctgccg taaaaaccac tgtttctgtt caaattcttc aatcctaatt caattgcttt
      721 ttctgaaact ggaaaaacgt gttgcaagca atccaacgct ttgtgcacca accgataatt
      781 ggcatgttgg taatttccta acaaaggaaa attgatggga acttcaatgt tttcttcgga
      841 tgcaaaataa attggagcgt gttgtgctgt tgccacttct ttaaaaacag gcgcagcttc
      901 tgggtggtat tcacctatca caacgggcgt attggctttt ataattcccg ctttttcacg
      961 tgcaattgct gcaatggtat tgcccaaaaa ttgtgtatgc tctatgctaa tgttagtaat
     1021 caacgaaatt tctggctgta tgcaattggt ggcatctaga cgtccgccca accctgtttc
     1081 tatcacacaa acactgcaat tttgttgctg aaaataaagt aaagccaata aaaaactaat
     1141 ttcaaaaaag gaaggttgaa tagttaattg tgccgattgg atagcttgca cttggtctat
     1201 cacaaattgt tcagtagcca tttccccgtt tactcgtatg cgctctcgaa aatcgtgtat
     1261 gtgtggagag gtaaataacc ccacagtttg cccagcttct tttaaaatgg atgccaacat
     1321 ggagcaagtt gaacctttgc cattggtccc agcaacatga atgaatttta gatggtggtg
     1381 tggattacca aagtgattca acaacagatg tacgttctcc aatccaggtt tataggccga
     1441 ggcaccaatc ttttgataag caggaaattg ttgaaacaac caatcaacag tatcttgata
     1501 cgttaatgcc aattgaattt tattgcggtt gaatccaaat gtggtaatat tcttcggtaa
     1561 tgcttgatcc tttagcatat ttcaactgcg ccttgcaagc cgaagcaacc ttacttataa
     1621 tactttgatc gttggttgta gtttctttac caacaacgcg agttccaatt acttttccct
     1681 cttcattgac ttgcaaaatc aacgttactt taatcaatga attggtgtaa atgttagaag
     1741 ttgtaatttc gttgtagcgt acacgtgctt ttccgctacc acttccatca ccacttcctg
     1801 ttccagttcc tttacctcct ttaccattat cgttaccaat aacaccaccg cttccagagt
     1861 catttccacc acctgagcca ccttttccac caaaaattgg attggtgttg tttgttgttg
     1921 ttgccgtgtt ttgagatggt ttttctgaag ttgttttatt ggatttcccc gactgaatag
     1981 cagttgcatt agggtcagtt gtagttacaa cacctgtttg ttgtggtgga ttggttttat
     2041 tcaaaatttc atccgatgct tttccgctac caccaggtga gcctccttgg tcttttgttg
     2101 gatcaaaatt taattcaatg aattctactg gaacttcttt cgttaattca gaatgaactg
     2161 gtgggtctgt tacagtagtt actgtaatga gactaatcaa caataggaga ataagaaaga
     2221 tagcgcttgt aaaaatgcgc gattttgtaa cttcttgctt atttgatacg attaccagtt
     2281 ccatactatc attttatacg tgttacagct gattttgttg catcgtttaa cgaatttttg
     2341 caatctcata gcttgctata aacttattca cggtaaaatt acaattgtaa aacacaagaa
     2401 atttgctgcc aatgaattat cattaacaac gaattgtgaa atatggaaaa taagattata
     2461 aaccttttag ttcaactgct ctcaatgtgt taatcatcaa cgaagcaatg gtcattggtc
     2521 ctactccacc aggaactggc gtaatgaatg aacattttgg agctacgttt ttaaaatcaa
     2581 catcgccaca caaacgccat ccacgttcac gcgttgcatc ctctacacga gtagttccaa
     2641 cgtccaccac aacagcgcct tctttaacca tttctgcggt aacaaactct ggtttcccta
     2701 ttgcagcaat gataatgtct gcttgttggc aaatttctgt aaggtttttt gttctactgt
     2761 gcgttaaagt caccgtgcaa tttccaggat tggtatttct tgctaaaaga atgctaactg
     2821 gcgaacctac aatgttgctt ctaccgatta caacgcaatg cttacctgct gtctcaatat
     2881 tgttgcgttt taaaagttcg atgattccag ctggtgttgc aggtaaaaac cctggtaaat
     2941 taagcaccat gtttcctaaa ttttgagggt gaaatccatc cacatctttt gaaggcaaga
     3001 cagcttgggt aacttttaac tcgtcaatgt gttttggtaa aggcaattga acaataaaac
     3061 catcaatgtt tgggtcttca ttcaattcgt tgattttttt caacaatgct tcttcggtaa
     3121 tagtatcatc gtaatgaatc aaggtgcttt caaaacctat ttcttcgcac gattttactt
     3181 tgttgtttac ataagtaagc gatcccccat catttccaac caaaatagct gctaaatgag
     3241 ggattttctt tccactttct tttctgtttt ttactgctaa agctaattct tcgcgaatgg
     3301 aagctgctgt ttttttaccg tctaataatt gcattgtatc taaaatctgg tacaaattta
     3361 ataaaactaa agcaatttag tagcgaaaga acagaagttt actaaataac accaattaat
     3421 tcgagcgttt ctttagcagc tgcttgttcg gctgcttttt ttgacggtcc aatacctaaa
     3481 ccatattttt tttcattcag caaaacaacc actttgtatt cccatgattg ccctgtattt
     3541 acctcgctaa ttacttgaaa atctattgaa aatcgttcgc gttgacaaaa aatatacaag
     3601 cgtgatttaa aatcaatttc ctcttccaaa attcggttga tgttgacgta ttttcggtaa
     3661 atatggcgtt tcaatgattt ttgaacagca tcaaatccac cgtctaaata aatagcacca
     3721 atgatagctt caaacgcatt tccttccaat gtattaatgt tgatggagcg ccctttgtgg
     3781 tagttgagca cgttacgaat ttccatttct tctgcaattt cagaaagggt ttttctactc
     3841 accaattttg attttacttt tgtgaggtaa ccttcatctt cttttgggaa acgatcgtaa
     3901 agcagacttg ccacaacggc atcaataatg gtatcgccaa gaaattctag gcgttcatta
     3961 gattctgcaa atttggtatt aacgttggat ttatgtgtta tagctttttc aaaaagggca
     4021 atgttttttg gtcgataacc aaaacgcgaa atgataaatt gcgttaaatc aagttgttta
     4081 tcatttctac cgaatctaaa gatgcgacca aattgaaaca attttatttt gaaaatttct
     4141 taacaattac acttgtatta tgtccgccaa acccaaaagt gtttgacaat gcaacattta
     4201 cagttctctt ttgtgcttta ttaaacgtaa agttcaactt aggatccaat tctggatcat
     4261 ctgtaaaatg gttgattgta ggtggaacaa tatcttcttt cactgccatg atgcttgcaa
     4321 ttgcttcgat tgcaccggca gcacctaata aatgccccgt cattgattta gtagaactaa
     4381 tgttcaaatc ataagcatgt tcaccaaaaa cttgttgaat agcttttact tctgcaatat
     4441 cccctaatgg tgtggatgtt ccatgaacat tgatgtaatc cacctcattt ggagaaatat
     4501 ttgcatcttc taaggctgcc aacattgtgt ttttagcacc taatccttct ggatgtggcg
     4561 ccgtaatgtg ataagcatca cccgacattc cacctccaat tacttctgca taaatttttg
     4621 cacctcttgc caaggcatgt tccaactctt ctaaaattaa agcaccagaa c
//
LOCUS       DAOQVQ010000143         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20495_length_4667_cov_3.560278, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000143 DAOQVQ010000000
VERSION     DAOQVQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20495_length_4667_cov_3.560278"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKN22_05590"
     CDS             complement(<1..480)
                     /locus_tag="PKN22_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNS42212.1"
                     /translation="MKNSFFFFFIMLAVQGFAQEFKTASNSSTIKSAIEKKHQATNSI
                     SAQFTEKVVSNMFKEPQKGYGNFLFKKENKVRWDKTSAKQLILINGETVKMYENGKAL
                     NNPTSQKIAKQIQGMMLSMLSGDFLNQKDFSISYEENSKLYRLTLSPKSPRMAKYIAK
                     "
     gene            complement(477..1238)
                     /locus_tag="PKN22_05595"
     CDS             complement(477..1238)
                     /locus_tag="PKN22_05595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNS42213.1"
                     /translation="MTRHHLNILGFIINCGWIAFFFQHQPMMWILLGMAALLFLVVLS
                     IGVVTPRFNYFVRQTTRLKSDRVLLTFDDGPDPKYTPQILSILQKHQIKALFFVIGKK
                     AEEHPAIVQQIIAENHLIGNHTQNHPLFFAGLGQEKVEKEIALCDNTLIAQNIHPGTY
                     FRPPVGYTNPRIARALKKFKKQTIGWSLRSYDTVAKDEQKLLQRLTSKVKSGDIVLLH
                     DNLAITTAILDDFITTAKKNGIKFANAQDIKSILA"
     gene            complement(1239..2144)
                     /locus_tag="PKN22_05600"
     CDS             complement(1239..2144)
                     /locus_tag="PKN22_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNS42214.1"
                     /translation="MSACIKMITSIGHQDSFLKENCWEHLVPLTNETEVISPDYKSYI
                     PPASLRRFSPVLRMALTAAQVCQNSVEQPFDAISVGTSLGCLRDTEKFLQTFITATGD
                     TLSPTAFIQSTHNTIAGAISMALGNHSYNMTHTQNSLSFETALMDGLLCIAEGKENVL
                     VGAADEAIDFLNELKPTVISSDLPFTSGATFMVLQPQSNTNSGKTIVDCVVYFNQDIE
                     NCIDQFLANNRVDKSAVELVLNAGNIPSNIGQKQIEYQQYTGLYYSSSAFAMHLANDF
                     NLSEGNYALIVNHQQEGKLGLTLLQ"
     gene            complement(2141..3316)
                     /locus_tag="PKN22_05605"
     CDS             complement(2141..3316)
                     /locus_tag="PKN22_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNS42215.1"
                     /translation="MQIFITGLGAISCIGQNVEEHLDALLAEKSGIKKGIHPYSQRYL
                     VGEIPLSNDELIAQFQLKVGGSRTGLLACVAAKEAFSGHAHHPEIRTGIISGTSVGGM
                     DISEVHYRNFMENHTHDVAQYSEHSSGNSSERIAQELGVGDFITTISTACSSAANAIM
                     LGARMIRAGKIDRAIVGGTDALTEFTINGFRSLMIFDEEWCRPFDESRKGLNLGEGAG
                     YIVLESEKSRRISNSVALAQLKGWSNASDAYHQTASSPEGYGATLSISEALKVAQLHP
                     DAIDYINAHGTATPNNDLSESKAIKTVFGNSIPPFSSTKAFTGHTLAAAGGLEAVFSV
                     LAIQKGYLFANLNHKNVIEETQLQPVVKTTKVERVRNVLSNSFGFGGNNSTIIIGEL"
     gene            complement(3320..3577)
                     /locus_tag="PKN22_05610"
     CDS             complement(3320..3577)
                     /locus_tag="PKN22_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNS42216.1"
                     /translation="MSELKETLKTQIISQLSLEDLTPADIADDMPLFAEDGLGLDSID
                     ALEFIVLLESNYGLKIADPSQGREIFHSVNSLADFIEKNRN"
     gene            complement(3588..4211)
                     /locus_tag="PKN22_05615"
     CDS             complement(3588..4211)
                     /locus_tag="PKN22_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNS42217.1"
                     /translation="MKIIASTYLSSKGAYLNGEKIVQFTDENWLKACYQHLALDYPKF
                     YKMDNLSKMAVLGVELLANSGIFSQIDTNDLELLFANSNASQHTDLKFIDSYTVAKNP
                     SPSLFVYTLPNILTGELAIKHKWYGENSFYISPNFDAQLFATKIHQAYKKGVNYCLCG
                     WIDSKLEANSTLSEESFLFLVALNEENASLHQEIEQLMTQYNQKEKE"
     gene            complement(4208..>4667)
                     /locus_tag="PKN22_05620"
     CDS             complement(4208..>4667)
                     /locus_tag="PKN22_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HNS42218.1"
                     /translation="YLGGASSNDANHISGPSRTGEGLFRAINKTIERNSIDKTNIDFI
                     SAHGTGTLYNDEMESIAFNRCELAHIPLNSLKGYFGHTLGAAGVIETLVAMFSIEHQL
                     LFANKGFEELGTSQPLSIVQSTHSSSVKTVLKTASGFGGGNAALLIRALS"
BASE COUNT         1384 a          892 c          861 g         1530 t
ORIGIN      
        1 tttagcaatg tatttagcca tgcgcggact tttaggcgat aaagttaaac gataaagttt
       61 ggagttttct tcatagctga tggaaaaatc tttttggttt agaaaatcgc ccgaaagcat
      121 tgataacatc atgccttgta tttgtttagc tattttctga cttgttggat tgttcaatgc
      181 ttttccattt tcatacattt taaccgtttc accgttgatt aaaatcaatt gcttggcgct
      241 ggttttgtcc catctaactt tgttctcttt tttaaacaag aaatttccat atcctttttg
      301 tggctctttg aacatgttgg aaaccacttt ttcggtaaat tgtgcactta tactgttggt
      361 agcttggtgt tttttctcaa ttgccgattt aattgtacta ctgtttgaag ccgttttgaa
      421 ctcttgcgca aatccttgaa cagccaacat tataaaaaag aagaaaaaac tatttttcat
      481 gctaaaattg atttaatgtc ttgtgcattc gcaaatttaa taccgttttt tttagctgtt
      541 gtaataaaat catccaatat tgctgtggtt atcgctaaat tatcgtgcaa taaaacaatg
      601 tcacccgatt ttaccttaga agttaaacgt tgaagcaatt tttgctcgtc ttttgcaacg
      661 gtgtcatacg agcgcaaact ccaaccaatt gtttgttttt taaatttttt cagtgcacgc
      721 gcaattcgtg gattggtgta tccaactggc ggacgaaaat aagtgcctgg atggatgttt
      781 tgagcaatca aagtgttgtc gcacaatgca atttcttttt ctactttttc ttgccccaac
      841 cctgcaaaaa acaagggatg attttgcgta tgattgccaa ttaaatggtt ttctgcaatg
      901 atttgttgaa caatagctgg atgttcttct gcttttttac cgataacaaa aaacaaagct
      961 tttatttggt gtttttgcaa aatagaaagt atctgaggtg tgtatttagg gtctggacca
     1021 tcgtcaaaag ttaataacac acggtctgat tttaatcgcg ttgtttgtcg aacaaaataa
     1081 ttgaaccgag gtgttacaac tccaatggat aatacaacca aaaacaacaa agccgccata
     1141 cccaaaagta tccacatcat cggttggtgt tgaaagaaaa atgcaatcca accacaattg
     1201 ataataaatc ctaaaatgtt taaatgatga cgtgtcattt attgaagtaa tgttaatcct
     1261 aattttcctt cctgctgatg attgacaatc aacgcataat ttccttccga caaattaaaa
     1321 tcgtttgcta ggtgcattgc aaatgcagat gatgaataat acaaacctgt atattgttgg
     1381 tattcaattt gtttttgccc gatgtttgaa ggaatatttc cagcattcaa aaccaattca
     1441 acagccgatt tatccaccct gttgtttgct aaaaattgat caatacaatt ttcaatatct
     1501 tggttgaaat atactacaca atccacaatt gttttgcccg aattagtatt agattgaggt
     1561 tgaagcacca taaaagttgc accagatgta aacggtaaat cggaagaaat gactgttggc
     1621 tttagttcgt tcaaaaaatc gatggcttca tcagccgctc caaccaatac attttctttt
     1681 ccttcggcaa tgcacaacaa gccatccatc aatgccgttt caaacgaaag actgttttgt
     1741 gtgtgcgtca tgttgtagga atgattaccc aaagccatag aaattgcacc ggcaatggtg
     1801 ttgtgggtag actgaataaa cgcagtagga gagagcgtat ctccagttgc ggtgatgaat
     1861 gtttgcaaaa acttttctgt atcgcgtaaa catcccaaag aagtgcctac tgaaattgca
     1921 tcaaatggtt gttctacact attttggcaa acttgagcag ctgttagtgc cattcgtaaa
     1981 acaggactaa atctgcgcaa tgatgcaggt ggaatgtagg atttgtaatc gggcgaaata
     2041 acttccgttt cattggtcaa cggcaccaga tgttcccaac aattttcttt taaaaaactg
     2101 tcttgatgac caatggatgt tatcattttg atgcatgcgc tcatagttca ccgattatta
     2161 tggttgaatt gttgcctcca aaaccaaaag agttggaaag tacatttcga acacgttcaa
     2221 cttttgttgt tttcactaca ggttgtaatt gcgtttcttc aattacattt ttatggttca
     2281 aattggcaaa taaatatcct ttttggatgg ctaaaacact gaaaacagct tctaaacctc
     2341 cagcggcagc caatgtgtgc cccgtaaatg ctttagtgga actaaacggt ggaatggaat
     2401 tgccaaaaac tgtttttatt gcttttgatt ctgataaatc gttgttgggt gttgctgtgc
     2461 cgtgtgcatt gatgtaatca atagcatctg gatggagttg cgccactttt aacgcttcag
     2521 aaatggaaag tgtagctcca tatccttctg gtgatgaagc tgtttggtgg taggcatctg
     2581 aagcattgct ccaacctttt aattgtgcaa gagcaacact gtttgaaata cgtctgcttt
     2641 tttcagattc aagaacgatg taaccagcgc cttctcccaa gtttaaacct tttcggcttt
     2701 catcaaaagg gcggcaccat tcttcgtcaa aaatcatcaa cgatcgaaaa ccattgatgg
     2761 taaattctgt caatgcatcg gttccaccaa cgatggctct atcaattttc cccgctcgta
     2821 tcatgcgtgc ccccaacatt attgcattgg cagccgacga acaagctgta gaaatggtgg
     2881 taatgaaatc gcctacacct aattcttgtg caatgcgttc acttgaattt ccacttgaat
     2941 gttcactgta ttgggcaaca tcatgcgtgt gattttccat aaaattgcgg tagtgcacct
     3001 cactgatgtc cattcctcca acagaagttc cagatataat gcctgtgcga atttctggat
     3061 gatgggcatg acccgaaaaa gcctcttttg cagccacaca cgctaaaagt ccggtgcgag
     3121 aaccacctac ttttaactga aattgagcaa ttaattcatc attggaaagt ggaatttcgc
     3181 ccaccaaata acgctgagaa taaggatgta tgcctttttt tattcccgac ttttcagcca
     3241 ataaagcatc caaatgttct tcaacatttt gacctataca gctaattgcg cctaatccag
     3301 tgatgaatat ttgcatgatt tagtttctgt ttttttcaat aaaatcagcc aatgaattta
     3361 ccgaatgaaa aatttcacgt ccttggctcg gatcggcgat ttttaaacca tagtttgact
     3421 ctaacaatac gataaattcc aatgcatcaa tggagtcaag tcctaaacca tcttcggcaa
     3481 acaacggcat gtcatctgca atgtcagctg gagttaaatc ttctaaagac aactgactga
     3541 tgatttgtgt ttttaaagtt tcttttaatt cgctcataat taataattta ttctttttct
     3601 ttttggttgt attgtgtcat caattgctca atttcttggt gcaatgatgc gttttcttcg
     3661 tttaaagcca ctaaaaataa gaaagattct tcactcaaag tggagttggc ttccaattta
     3721 gaatcaatcc atccacataa acagtagttt acaccttttt tataggcttg gtggattttt
     3781 gttgcaaaca attgagcatc aaaattaggt gaaatgtaga atgaattttc tccataccat
     3841 ttgtgtttga ttgccaattc acctgttaaa atattgggca aagtgtacac aaacaacgac
     3901 ggacttggat tttttgcaac agtgtaagaa tcaatgaatt ttaaatcggt gtgctgactg
     3961 gcattggagt tggcaaacaa caattccaaa tcattggtat caatttgtga aaaaatacct
     4021 gagttggcaa gcaattccac acctaaaacg gccatttttg ataaattatc cattttataa
     4081 aattttggat aatccaacgc taaatgctga tagcacgctt ttaaccaatt ttcatcggta
     4141 aactgaacga ttttttcacc gttcaaatac gctcctttgg aggataaata ggtacttgca
     4201 attattttca tgagagcgca cgtattaaca aagcggcatt tccaccacca aaaccagatg
     4261 ctgtttttaa aacagttttt acacttgaag aatgtgttga ttgaacaata gatagcggtt
     4321 gcgatgttcc taattcttca aatcctttgt ttgcaaaaag aagttggtgt tcgatggaaa
     4381 acatagcaac caatgtttca ataacgccag ctgctccgag agtgtgccca aaatagcctt
     4441 tcaaactgtt gagcggaatg tgtgccaatt cacaacggtt aaaagcaatg ctttccattt
     4501 catcattgta caaagttccg gtgccatgtg ccgatataaa atcgatgttt gttttgtcaa
     4561 tagaattgcg ctcaatggtt ttgttaatgg cacgaaaaag tccctcacct gtgcgcgaag
     4621 gtcccgaaat atgattggca tcattggaag aagctccccc caaatat
//
LOCUS       DAOQVQ010000144         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20720_length_4628_cov_3.803193, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000144 DAOQVQ010000000
VERSION     DAOQVQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20720_length_4628_cov_3.803193"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..748)
                     /locus_tag="PKN22_05625"
     CDS             complement(26..748)
                     /locus_tag="PKN22_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNS42219.1"
                     /translation="MGRAFEYRRAAKEKRWDKMSKLFPKLGKAITMAAKEGGPDPDTN
                     SALRTAIKNAKAQNMPKDNIDAAIKRANAKDAETFSNINYEGKGPHGVLVFVECATDN
                     PTRTVANVKSYFNKAGGGVVPNGSLEFMFNRKAVFEFAKTDSINLEELELELIDAGLE
                     EIEQVDEKVFVYGDYTSFGELGHALENLGIDVEKSTLQRIPTSPVEFTEEQLADIEKM
                     LDKIEDDDDIQQVFTMLLKLIN"
     gene            1686..3209
                     /gene="gltX"
                     /locus_tag="PKN22_05630"
     CDS             1686..3209
                     /gene="gltX"
                     /locus_tag="PKN22_05630"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNS42220.1"
                     /translation="MSEKPVRVRFAPSPTGPLHIGGVRTALYNYLFAKKNNGTFIIRI
                     EDTDQTRFVPGAQDYIMDALKWCGINATEGPGIGGEYGPYVQSERNDMYRPYAEQLVA
                     NDKAYYAFDTPEELEEMREKAKLMNMPNWQYNSVTRTSMKNSISLPADEVKRRIENGD
                     PYVIRIKMPRNVDVRFEDMIRGWVTVNTNNLDDKVLFKSDGIPTYHLANIVDDHTMEI
                     SHVIRGEEWLPSAPMHIMLYEAFGWEPPRFAHLPLLLRPDGNGKLSKRDGDRLGFPVF
                     PCDWVTAEGELYSGYREKGYLPQAFINMLAFLGWNPGTTQEIFSMEELEAIFTLERVS
                     KGGAKFDPDKTKWFQHQHLKAVPDAELADLLIAQYDAKVDKSRLTQIVHLMKERATFP
                     QDILTEGAYLMERPTSIDAQSIEKKWKDDSNDLLTGWLAQLEVISDFTAPSIENVFKA
                     YIAEKNVGIGAVMFLFRLLVTGQGAGPSMFEISEFLGKQEVIDRLNWGLATVAEMKK"
     gene            3213..3566
                     /gene="folB"
                     /locus_tag="PKN22_05635"
     CDS             3213..3566
                     /gene="folB"
                     /locus_tag="PKN22_05635"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNS42221.1"
                     /translation="MKNHIEVNGIKCYAHHGCLEEEAKIGGHYVIDVALVTDFSEAMK
                     TDDLTKTICYVDINTIVKEEMAIRSKLIEHVGGRIFNRIKNEINNVDHLRVKIVKICP
                     PINGDVDNCSIIIED"
     gene            complement(3630..4412)
                     /locus_tag="PKN22_05640"
     CDS             complement(3630..4412)
                     /locus_tag="PKN22_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42222.1"
                     /translation="MNLSISVNASKGCGPVTAQFSSNATSPIQLTWDFGDGTTSNAYN
                     PTHIYNSENCYAVTLTADNGLGCITTVSLNDSICVYAQPNASFWATPATIENYPYEVQ
                     VNNNSTNATNYTWYFGDDTAPSHSFEPSHTYASDASGGFTIMLVASNAIGCVDTAYYG
                     VKIIEDLIFYVPNSFTPDGDQFNNTFKPVFTAGFDKYNYEMIIYNRWGEKVFQTHNTE
                     IGWDGTYNGKMVQDGTYTWKIIVKVSDYDEHKQFVGHVNMIR"
BASE COUNT         1473 a          786 c          849 g         1520 t
ORIGIN      
        1 acacaataaa aatgaatttt tgctgttaat taatgagctt tagcaacatc gtgaaaactt
       61 gttgaatatc gtcgtcgtct tcaattttat ccaacatttt ttcaatatcc gccaattgtt
      121 cttcggtaaa ctcaactggc gaagttggaa tacgttgtaa cgttgatttt tcaacatcaa
      181 ttccaagatt ttctaaggca tgacctaatt caccaaaaga agtgtaatcg ccgtaaacaa
      241 atactttttc atctacttgt tcaatttctt ccaatccagc atcaattaac tctaattcca
      301 attcttctaa gttaatactg tctgtttttg caaattcaaa tacggctttg cggttaaaca
      361 taaattccaa agatccgttt ggaacaacac caccaccagc tttgttaaaa taggatttca
      421 cgttggctac agtacgggtt ggattgtcgg ttgcacattc aacgaaaacc aaaacaccat
      481 gcggaccttt tccttcgtag ttgatatttg agaatgtttc agcgtctttg gcattggcac
      541 gtttgatggc tgcatcaata ttatctttgg gcatgttttg cgctttcgca tttttgattg
      601 ccgtacgtaa agcagaattg gtatctggat caggaccacc ttcttttgcg gccattgtaa
      661 ttgcttttcc taattttgga aataacttag acattttgtc ccaacgcttt tcctttgctg
      721 ctctgcggta ttcaaacgct cttcccatgt tgtatttcta tttaataaat tctattatag
      781 aaggcaaaaa tataaaaatc aataaataaa tgcaacgtac attggaagaa tgctctattt
      841 caaatagaaa agaaaatatc tataccccta taaattattg aaattaattc aaaaaaacct
      901 aaatctaaac cattttaatt tggttttata aaaatgttgc tcggtatttt cttgttagaa
      961 acaagtaaat tgatttctgg tagttgggtg gtagcaatta aaaatactaa aacttcaccc
     1021 aaggggttta taaataaatc tcttgttccg tattatatga aattgtttta caggaattat
     1081 tagttgtctt tcgatttttt gaaatgaagt catatatggg atttcttatg aattttggaa
     1141 atatttgaat aaagtatcct aagaacttaa atttccaatt acatcttttt aaaattccta
     1201 taattgcttc agatttgatt tcatattgtg cggttttttt atctattaag tagattgtat
     1261 ctttatgtgg attcaatgat ataccaattt cagaacaaat attttctcca gcctttgaat
     1321 gactggaaat gaagatgaat ttatttttac tgtatttcaa tggaatgcgt ataaaattat
     1381 tgcatagcaa acattgtcca tcaaaaagta ttattaattt attttgttct atcatttcaa
     1441 aaagactatt aaaggcttaa attcaagtta taaatgcaac aaacctataa ttcctgttta
     1501 taaagatata aaatatccga ttgtttatag taggatttat caattctatt tttaaaaatg
     1561 attgccagca acaacagttg gttttgctgt gtcgcatagg cttttaatta tcggcaatta
     1621 aaagagcaga attaatttct ttttttccta attgctttac tatctttgca aaaaattcaa
     1681 aaaaaatgtc agaaaaaccg gttagggttc gttttgcacc atctccaaca ggacctttac
     1741 acattggagg tgtgagaacc gcattgtaca attatttgtt tgcaaagaaa aacaacggaa
     1801 catttatcat tcgtattgaa gataccgacc aaactcgttt tgttcccgga gcacaagatt
     1861 acatcatgga tgcattgaaa tggtgtggaa ttaatgctac cgaaggacca ggaattggtg
     1921 gtgaatacgg accttatgtc cagtcagagc gcaacgatat gtaccgcccc tatgccgagc
     1981 aacttgttgc aaacgacaaa gcttactatg cttttgatac gccagaagaa ttggaagaaa
     2041 tgcgcgaaaa agctaaattg atgaacatgc ctaattggca atacaacagt gttactcgta
     2101 catcaatgaa aaactccatt tcgcttcctg cagatgaggt aaaaagaaga attgaaaatg
     2161 gtgatccgta tgttattcgt atcaaaatgc cgcgcaatgt ggatgttcgt tttgaagaca
     2221 tgattcgcgg atgggtaacg gtaaacacca acaacttaga cgataaagtg ttatttaaaa
     2281 gcgatggtat tccaacctat cacttggcaa atattgtgga cgaccatacc atggaaattt
     2341 ctcacgttat tcgtggagaa gaatggttgc cgtctgctcc tatgcacatc atgttgtatg
     2401 aagcatttgg ttgggaacca cctcgttttg ctcacttgcc gttgttgttg cgtcctgatg
     2461 gaaatggaaa attatctaaa agagatggcg accgtttagg tttccctgtt ttcccatgcg
     2521 attgggtaac tgcagaagga gaattgtatt cgggctaccg tgaaaaagga tatttgccac
     2581 aagcatttat caatatgttg gcatttttag gatggaaccc tggaacaaca caagaaattt
     2641 tctcaatgga agaattagaa gcaatcttta ctttggagcg tgtttctaaa ggtggtgcaa
     2701 aattcgaccc agataaaaca aaatggttcc aacaccaaca tttaaaagct gttccagatg
     2761 cagaattggc tgatttgtta attgctcaat acgatgcaaa agtggacaaa tcacgtttga
     2821 cacaaattgt gcatttgatg aaggaaagag ctacttttcc tcaagatatt ttaaccgaag
     2881 gagcttattt gatggaacgt ccaacaagta ttgatgcaca aagcattgag aaaaaatgga
     2941 aagacgattc caacgattta ttgacgggtt ggttggcaca attagaagta atttctgatt
     3001 ttactgctcc atcaatcgaa aatgttttta aagcgtatat tgctgagaaa aatgttggta
     3061 ttggtgcggt gatgttcttg ttccgtttat tggtaacagg tcaaggggct ggaccaagca
     3121 tgtttgaaat atctgaattt ttgggcaaac aagaagttat cgaccgttta aattgggggt
     3181 tggcaacagt tgccgaaatg aaaaaataat tgatgaaaaa tcacatagaa gtcaatggta
     3241 ttaagtgcta tgcacaccat ggctgcttag aagaagaagc taagattggt ggccattacg
     3301 taattgatgt tgcgttggtc accgattttt cggaagccat gaaaaccgat gatttgacaa
     3361 aaaccatttg ctacgtggac atcaatacta ttgtaaaaga agagatggct attcgttcca
     3421 aattaattga acacgtgggt ggacgaattt tcaatcgtat taaaaacgaa atcaacaacg
     3481 tagatcattt gcgtgtgaaa atcgtaaaaa tttgtccacc aattaatggc gatgtagata
     3541 attgttcgat aattattgaa gattaagctt atacttaatt ataaacaaaa gaggctgatt
     3601 ttttcagcct cttttgtttt cattgtgttt tatcgtatca tgttgacgtg tcctacgaat
     3661 tgtttgtgtt cgtcataatc actaactttt acaataattt tccacgtata ggttccatct
     3721 tgaaccattt ttccattgta tgttccatcc cacccaattt ctgtattgtg cgtttggaaa
     3781 accttttcac cccaacggtt ataaataatc atttcatagt tatatttatc aaaccctgct
     3841 gtgaaaacag gcttgaaagt attattaaat tgatcaccat ctggggtaaa tgaatttgga
     3901 acatagaaaa tcaaatcctc aataattttc acgccataat atgcagtgtc aacacagcct
     3961 attgcatttg aagcaaccaa cattattgta aaaccaccac tagcgtcact tgcgtatgtg
     4021 tgagatggtt caaaagaatg tgaaggagct gtgtcgtctc caaaatacca agtataattg
     4081 gttgcattgg ttgaattatt atttacctga acttcatacg gataattctc aatagtagct
     4141 ggagttgccc aaaaacttgc atttggctgt gcataaacgc aaatagaatc atttaatgaa
     4201 actgtagtaa tacagcccaa gccgttgtct gctgttaatg taactgcata gcaattttct
     4261 gaattataaa tatgcgttgg gttgtatgcg ttggatgtag ttccatcgcc aaaatcccat
     4321 gttaattgaa taggagatgt ggcattgcta ctaaattgag cagttacagg tccacatcct
     4381 tttgaagcat tcacagaaat tgaaagattc attgcatcaa caacggttac tgttacagat
     4441 tctgatgcag aacatccatt tgaatttgtt cctactaccg tataagttgt tgttgctgac
     4501 ggagaaacaa cttgtgtaga gccaatgaat caattatccc aattataagt gttggctcca
     4561 tttgcactaa ttgttgtgtt ggctccagcg caaatagtaa catcattact tactgtcaaa
     4621 actggatt
//
LOCUS       DAOQVQ010000145         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20785_length_4616_cov_3.510414, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000145 DAOQVQ010000000
VERSION     DAOQVQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20785_length_4616_cov_3.510414"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKN22_05645"
     CDS             complement(<1..565)
                     /locus_tag="PKN22_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42223.1"
                     /translation="MATLLHNPNFSEDVQLYLDELRIKLAYRMSTMFLIVFAILTYAY
                     YFDSVESFLTMAFGFVLSVFCLVIMYYKKNYQLVFILYSVLGVFVTSFALIFFHETIH
                     LVDVLWMLAAVSLAFLGVGRRVGIILLFVSLICIAIFIFYSLNIHIEAVKPRTFYQKI
                     ALVTEMISGFSLNFYLFYLFTNVSRYSA"
     gene            681..2786
                     /locus_tag="PKN22_05650"
     CDS             681..2786
                     /locus_tag="PKN22_05650"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNS42224.1"
                     /translation="MRKIVFIVLILSTSSTFIHAGRIENAFEALHIYDYFKAKKYFTK
                     SLKSQEAIGAFGLATIFYRKDNPFHSLDSAYVYINKAYNSYPAIKKNAQQRYISFVNE
                     TKIDSLRTLVSSQFFTLAKQENTIESYQHFIDKHNWAKELGTAIHLRDSIAFLRTKES
                     GMSTDFVNFLKKYPTSDFAAEAEAMLMDAQFNEVTASKTLDSYELFLKSFPRNTNKDK
                     AETAIYEIVTKPNTVEVLELFLNKYPDNHLKNLAWERLYQLSVYDYSEKSIKRFIEKY
                     PAYPYKDKIQTDLEFMNFAIYPFEKNGLFGFMDSTGKAVIQPIYTSVNPFKEGLAMVS
                     KNESYGYIDKKGNVVIDFIYSGGNDFDQGRAVVEKNGRVGMIDRTGIVLFPTEFKELG
                     TISEGMIYGLKDSLFGYYNTMGDQVIAERFTDAFSFSKGLAKVEEKGLQAYIDTKGNY
                     VVYPAFEEIDFFSDSLLIFGDDEMYGLMKRSCQIVVPNKYDRIGKLVDGMAIVEQNGL
                     IGYINQRGEVVITPQYDPIPNFMERCQFANGSAVVSKNGQYGVITTGNKEIIPFKYDE
                     IGAFTQQIAVKKKGKWGYINRANAMVIQPTYGFAESFVNDLAIVQDYSLMGVIDKNGT
                     KIIETGYNNISITKSGYLIINNGALYGVGSKKGEMVVPMIYRSIRVFDDNLLILTDKD
                     GISYFDCKTKKIIKPTENE"
     gene            2788..3564
                     /locus_tag="PKN22_05655"
     CDS             2788..3564
                     /locus_tag="PKN22_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42225.1"
                     /translation="MLAGLDKYKYGIIAMLLFYIGATTIRIQYGFVERPDLSDLLQQN
                     PQEVIELKLESPEVVEQLMNATGEVKNAINDENSNGQSSNGSDDSYQQESYYGNAKSL
                     KDVENSVYDLEKSFFNETGGAQSRAQIQSDMDKRKKEQEELNKSKNTANKTPGASSNS
                     VGDSQKGMVLVSYNLKDRKGQYVPAPGYMCPQGTTGKVVVTVKVNQAGNVIEAKVNGA
                     TSSDDCMTSYALSFAKKSRFNYSSTANATQEGTITYTYVN"
     gene            3575..4183
                     /locus_tag="PKN22_05660"
     CDS             3575..4183
                     /locus_tag="PKN22_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS42226.1"
                     /translation="MTTIRIEDYFGNYFQQDVAPLFHDLFQNTSLIKRAKVEKGTFLM
                     RQGDICQQTIFVEKGLLRYYSIDAKGKEHILHFAPEGWLINDKDSAYFQLPTNYFIEV
                     LEDSEIVFLQLDFFNALVDKLPTIKNMNERLLHNHVRQLQKRITLLLSATAEERYVDF
                     IEIYPNILARVPQWMVASYLGITPESLSRVRKELAEKWKNTN"
     gene            complement(4176..>4616)
                     /locus_tag="PKN22_05665"
     CDS             complement(4176..>4616)
                     /locus_tag="PKN22_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNS42227.1"
                     /translation="KKREKKKYSKKMANFLKDEFTYSIRDLYTSEGRLLMQLVHRETG
                     KTVNQIISEYAGDFQAFIYRNLAKMFDQDLNSTYNPEKENYYTEIVINDILMGQVEFN
                     PQMDALTKEEFKISQKQYREERKHMRIKNREYKKEKRQKEKKAS"
BASE COUNT         1579 a          746 c          856 g         1435 t
ORIGIN      
        1 gagctgaata cctgctcaca ttggtaaaca agtaaaacag ataaaaattc agcgaaaatc
       61 ctgaaatcat ttcggttaca agcgctattt tctggtaaaa agttcgtggt tttaccgctt
      121 caatatgtat gtttaacgaa taaaaaataa aaattgcgat acaaatcaac gaaacaaata
      181 gtagaattat gcctactctt cttcctactc ctaaaaatgc caatgaaaca gctgctaaca
      241 tccataagac atctactaaa tggatggttt catgaaaaaa tatcagtgca aaggaggtca
      301 caaacacccc caacacactg tataaaataa acaccaattg gtagtttttt ttataataca
      361 ttattactaa acagaataca cttaatacaa aaccaaatgc catggttaaa aagctttcaa
      421 cagaatcaaa gtagtatgca tacgttagta ttgcaaatac tatcaaaaac atggttgaca
      481 tgcgatatgc caacttaatc cgtagttcat ctaaatagag ttgaacgtct tccgaaaaat
      541 ttggattatg taataatgtg gccaaaatat aatttttttc gcgagttagt ttttatcaaa
      601 tttatagctt tttttaaaaa atactaatta tttcttaaaa ttcattggat tgcaggctat
      661 tttataactt tgcactaatt atgagaaaaa tagtttttat agtattgatt ttatctactt
      721 ctagtacttt tattcatgct gggagaattg aaaatgcgtt tgaggcattg catatttacg
      781 attattttaa agccaaaaaa tattttacaa aaagtttaaa atcacaagaa gctattggag
      841 cttttggttt agcaactatt ttttatcgaa aagacaatcc ttttcattcg ttagactctg
      901 catatgttta catcaacaaa gcatacaaca gctatcctgc tattaagaaa aatgcgcaac
      961 aacgctatat ttcttttgtc aacgaaacta aaattgacag tttaagaacc ttggtgagtt
     1021 ctcaattttt tacccttgca aaacaagaaa acacaattga aagttaccaa cattttattg
     1081 ataaacacaa ttgggcaaag gaattaggaa cagccatcca tctaagagat agcattgctt
     1141 ttttacgcac caaagaaagt ggaatgtcaa cagattttgt caactttttg aaaaaatatc
     1201 caacctctga ctttgctgct gaagccgaag caatgttaat ggatgcacaa tttaacgaag
     1261 ttacagcaag taaaactttg gacagctacg aattgttttt aaaatcattc ccacgcaaca
     1321 ccaataaaga caaggcagaa acagccattt atgaaatcgt caccaaacca aatacggtgg
     1381 aggtattgga actattttta aacaaatatc ccgataatca tttaaaaaac ttggcttggg
     1441 aacgcttgta ccagttgagt gtgtatgatt attccgaaaa atcaatcaaa agattcatcg
     1501 aaaaatatcc tgcttacccg tacaaagaca aaattcaaac cgatttagag tttatgaatt
     1561 ttgccattta tccgtttgaa aaaaatgggc tgtttggatt catggatagc actgggaaag
     1621 ctgtaattca accaatttac actagtgtca atccgtttaa ggaaggatta gcaatggtga
     1681 gtaaaaacga atcctatggc tatatcgaca aaaaaggaaa cgtggtgata gattttattt
     1741 attcgggagg caatgatttt gaccaaggaa gagctgttgt ggaaaaaaac ggccgtgttg
     1801 gaatgataga tcgaactgga attgtattgt ttcctactga gtttaaagaa ttaggcacta
     1861 tttcagaagg aatgatttat gggttaaaag atagtttgtt tggctattac aacaccatgg
     1921 gtgatcaagt tattgctgag cgttttacgg atgctttttc attctccaaa ggtttggcaa
     1981 aagtggaaga aaaagggtta caagcctaca ttgatacaaa aggaaattac gttgtttacc
     2041 ctgcttttga agaaattgac tttttctccg attcgctttt gatttttggc gatgacgaaa
     2101 tgtatggttt gatgaaacgc tcgtgtcaaa ttgtggtgcc taacaaatat gaccgtattg
     2161 ggaaattggt ggatggcatg gcaatagtgg aacaaaatgg tttgattggc tatatcaatc
     2221 aacgaggtga agtggtaatt acaccacaat acgatccaat tcctaatttt atggaacgtt
     2281 gtcagtttgc aaacggaagt gcagttgttt caaaaaatgg tcaatatggt gtaattacta
     2341 ctggtaacaa agaaattatt cctttcaaat acgatgaaat tggtgctttt acccaacaaa
     2401 tagcagttaa aaagaaaggg aaatggggct atatcaatcg agctaatgca atggtaattc
     2461 aacctacgta tgggtttgca gaaagttttg taaatgattt ggccattgtg caagattatt
     2521 cattaatggg cgttatagat aaaaatggaa caaaaattat tgaaaccgga tacaacaata
     2581 tttccatcac caaaagtggt tatttaatca tcaacaacgg agctttgtat ggtgttggtt
     2641 ctaaaaaagg tgaaatggtt gtgccaatga tttatcgttc gatacgtgta tttgatgata
     2701 atttattaat tttaacggat aaagacggaa taagttattt tgactgtaaa accaaaaaaa
     2761 taataaaacc aactgaaaat gaataacatg cttgcaggac ttgacaaata taaatacggc
     2821 atcattgcga tgttgctgtt ttatattggc gcaaccacca ttcgcattca gtatggtttt
     2881 gtggaacgac cagatttaag cgatttgtta caacaaaatc cgcaagaagt tattgaatta
     2941 aaattagaat ctcccgaagt tgtggagcaa ctgatgaacg ccacaggtga ggttaaaaat
     3001 gctattaacg atgaaaacag caacggtcag tcttctaacg gcagcgatga ttcttaccaa
     3061 caagaaagtt attatggcaa tgccaaatcg ctaaaagatg ttgaaaatag tgtttacgat
     3121 ttggaaaaaa gttttttcaa cgaaacaggt ggagcgcaaa gtagagcgca aatccaaagc
     3181 gacatggaca agcgtaaaaa agagcaagaa gaacttaata aatcaaaaaa cacagcaaat
     3241 aaaaccccag gtgcttcttc taacagtgta ggcgattctc aaaaaggaat ggtgttggtg
     3301 agctataatt tgaaagatag aaaaggacaa tacgttccag caccgggcta catgtgtcca
     3361 caaggaacca ctggaaaagt agttgttacg gttaaagtaa atcaagctgg aaatgttatt
     3421 gaagcgaaag taaatggtgc aacttcatcg gatgattgta tgacttccta cgcattgagt
     3481 tttgcaaaaa aatcgcgttt taattattct tctacagcaa atgcaacgca agaaggaaca
     3541 attacttata cctacgtcaa ttaactttta gcctatgaca acaattcgga ttgaggatta
     3601 ttttggcaat tattttcaac aggatgttgc gcctttgttt catgatttgt ttcaaaatac
     3661 tagtttaatt aagcgtgcaa aggttgaaaa aggaactttc ttaatgcgtc aaggggatat
     3721 ttgccagcaa acaatttttg tagaaaaagg gttgttgcgc tactactcta ttgatgccaa
     3781 gggtaaagaa cacatcttgc attttgcacc agaaggttgg ctaatcaacg acaaagacag
     3841 tgcttatttt caattgccta ctaattattt cattgaagtt ttggaagaca gtgagattgt
     3901 ttttcttcaa cttgattttt ttaatgcttt ggtcgataaa cttccgacta tcaaaaacat
     3961 gaacgaacgt ttgttgcaca accatgttcg ccagttgcaa aaacgcatca ccttgttatt
     4021 gagtgccaca gccgaagaac gctacgtgga ttttatagaa atttatccca atatacttgc
     4081 acgtgtaccg caatggatgg tggctagtta cttgggcatt acccccgaaa gtttaagtcg
     4141 cgtgcgcaaa gaattagccg agaaatggaa gaatactaac tagctttttt ctctttttgg
     4201 cgtttttctt ttttgtattc tctgttttta atgcgcatgt gtttgcgttc ttcgcggtat
     4261 tgcttttgag aaattttaaa ttcttctttg gttaaagcat ccatctgcgg attaaattct
     4321 acctgcccca ttaatatatc gttgataaca atttcggtat aataattttc tttctcgggg
     4381 ttgtaggttg aattcaaatc ttgatcaaac attttggcca agttgcggta aataaacgcc
     4441 tgaaaatcgc ctgcatattc tgaaataatt tggttgactg tttttccagt ttcacgatgt
     4501 accaattgca ttagcaaacg cccttcactt gtatataaat cacgaataga ataggtaaat
     4561 tcatctttaa ggaaattcgc cattttttta gagtattttt tcttttcgcg tttttt
//
LOCUS       DAOQVQ010000146         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20880_length_4600_cov_4.109131, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000146 DAOQVQ010000000
VERSION     DAOQVQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20880_length_4600_cov_4.109131"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1645
                     /locus_tag="PKN22_05670"
     CDS             104..1645
                     /locus_tag="PKN22_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNS42228.1"
                     /translation="MKKTVAIHIKGFPFTIEEEAYQRLESYIKRLESVLKGEEGADDI
                     VEDIELRIAELLSKKLTGSKSIIELSDVEDVLNTLGDPSDYATEEGTEKETTSNYKAE
                     YDNTSYERRLFRDTESSFIGGVCSGLSNYFGIDVTLIRLVWAIAILLGGVGLFLYIVL
                     WIIIPKATSSIDRLKMKGQPINVDTVKEEVDRAAKNISEKSRHFANSIKNNKNIQNAG
                     SSIKRFFSLCFGFFFTAIGISLLIAFIGLVIGRPQIIPSSTDAGFLSITGLANLVVAN
                     PADVQLSWISFYVFAISITLFFLIGGFVLLFNINNKWYRYVNFVLIALSVIGFTLGMV
                     VFSRTGRDFAINGELEKEIAAVNSNQLVVLPVTSSSTTSSNYKIKSSNMWDIYIGKES
                     ISVSDVSIKYKPSSDSLFHVTIEKEANAATLNNAVRKAENIRYNAEMKGDSLFLPANY
                     SFPKSDKFRNQDVDVIIYIPWGKTVKIGNSIIDLSKKTTVFEDDDDWDQQGEISQDGT
                     YYHFD"
     gene            complement(1724..3979)
                     /locus_tag="PKN22_05675"
     CDS             complement(1724..3979)
                     /locus_tag="PKN22_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42229.1"
                     /translation="MKQILLLIFIVQTALIFGQIQGDGGKSTDWSSLQSTLPVVQFLQ
                     PDIKALQAEDAKNDRLGEGPWRFGYNNETSLTLNNSGTWLSLPNGGKLWLLQLECVDA
                     TTVNLTLEKTQIPEGNALFVYNPNKSFILGKFTQKHIYNGQLGTELIPGSKVIVEYYV
                     APENNDNQGELTIATVTHGYRTAEEFQEKAFGQSGACHKNVNCPEGTPFANQKRSVVM
                     LVSGSNGFCTGALVNNTAYDGKPYVLTANHCYSNPASWVFRFNWESPDCNNPGSSPSF
                     TSMSGAVLRARRSTSDFCLVEITGGLESGTVPAAYNAYFSGWDKTGENPTSTFGIHHP
                     KGDIKKISFDDQQSVPAQTKVGSVVSDVNGTWRVQWDRNTTTEGGSSGSPLFNQHKRI
                     IGQLWGGQASCSNTDGFDYYGRFSISWNPTGSDSSTQLKYWLDPSNTGALVVNGYQPG
                     ESIQLDGSLVNLKNVSGTVCDAVVEPKITLVNTGQTTLTSATITYGLDGVENQTYNWS
                     GNLTMFQSQEITLPTITATAGGHSFSATITNVNGGSDEVADNNKMTSNLYVVTNPNTV
                     TLDVTVDCYASETSWQITNASNDTLYKSPVYYNTDEGLHTYSLCLSEGCYSFKIFDSY
                     SDGMSYCDSGMVVIKGPMNEILGQITKVEAATFGSSKIVNFCIGDNGISSIEADFGVF
                     PNPTSNQINWMSNDVKFVKMLDVSGKIVLQKAIATEHAISVNQLVDGVYFVEFTLTSG
                     TSVVKKIAVKK"
     gene            4113..>4600
                     /locus_tag="PKN22_05680"
     CDS             4113..>4600
                     /locus_tag="PKN22_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42230.1"
                     /translation="MLQFNKVFYLLTTCILISVSSCSTIKNAPSQRHGSCYLENRLPY
                     TLSSAPKPLIIENLDSNLIHSFSISSLNIANGIGVLDLLSEYVQHRNKYKSNPTFESR
                     LQAIELFQRIDYRINLASLEISSIASEMDCEEEKITQLADFMKEKEGQVENRLNVAAI
                     AVG"
BASE COUNT         1439 a          862 c          828 g         1471 t
ORIGIN      
        1 atttctcggt aactgaaaaa ggaatagaat ttactaaaaa actggaagaa acttggttgg
       61 agctaagcac aaccgttaca aaaatcacta aaaattaaag attatgaaaa aaactgtcgc
      121 tatacacatt aaaggtttcc cattcactat tgaagaggaa gcataccaac gtttggaaag
      181 ctacatcaaa cgtttggagt ccgtattgaa aggcgaagaa ggtgctgacg atattgtgga
      241 ggacattgaa ttacgaattg ccgaattgtt gagcaaaaaa ttaacaggtt ctaaaagcat
      301 cattgaatta agtgatgtgg aagatgtact aaacacactt ggcgatccat cggattatgc
      361 aactgaagag ggaacggaaa aagaaactac ctctaattat aaagccgaat acgacaatac
      421 aagctatgaa agacgtttgt ttcgtgacac ggaatcgagt ttcattggag gggtgtgttc
      481 gggtttatcc aattattttg gtattgatgt aactctcatt cgtttggttt gggccattgc
      541 tattttactt ggtggtgttg gattgttctt gtacattgtg ctatggatta tcatcccaaa
      601 ggcaactagt tcaattgaca gacttaagat gaaaggtcaa ccaattaacg tggatactgt
      661 aaaagaagaa gtggacagag ctgctaaaaa catttctgaa aaatcacgtc attttgccaa
      721 cagcattaaa aacaataaaa acatacaaaa tgctggttcc tccatcaaac gattttttag
      781 tttgtgcttt ggtttctttt tcacagccat cggtatttcg ttgctcattg cttttatagg
      841 attggtgatt ggtcgcccac aaattattcc atcaagcact gacgcaggat ttttatccat
      901 cactggctta gccaatctag ttgtagccaa cccagctgat gtacaattat catggattag
      961 cttttatgta tttgccattt ccattaccct ctttttctta attggcggtt ttgtattgct
     1021 gtttaacatt aacaataaat ggtaccgcta tgtcaatttt gtattaatag cactttctgt
     1081 gattggtttt actttaggaa tggtcgtttt ttcaagaacg ggtagagatt ttgcaatcaa
     1141 tggtgagctt gagaaagaaa ttgcggctgt aaattccaat caattggttg tgttgccagt
     1201 tacttcgagt tcaactacat ctagcaacta caaaattaaa tcgtccaaca tgtgggatat
     1261 ttatattggc aaagaaagta tttctgtttc agatgtatcc attaaataca agccttccag
     1321 cgatagttta tttcacgtga caattgaaaa agaagccaat gcggcaacac ttaacaatgc
     1381 tgttagaaaa gcagaaaaca ttcgttacaa cgcagaaatg aaaggtgatt ctttgttctt
     1441 accagcaaat tattctttcc caaaatcgga taaatttaga aaccaagatg tagatgttat
     1501 catttacatt ccatggggaa aaacggtaaa aattggcaat tcaatcattg atttgagtaa
     1561 gaaaacaact gtttttgaag atgatgatga ttgggaccaa caaggagaaa tcagtcaaga
     1621 tggaacgtat tatcatttcg actaataatt ctttaaaaat taaagaataa aaaaacggca
     1681 gttctatatt tggaactgcc gtttttatgt tcaactttat aacttatttt ttaactgcta
     1741 tttttttaac aacgcttgtt ccagatgtga gtgtaaactc cacaaaataa acaccatcca
     1801 ccaattgatt tacggagatt gcatgttcag tagcaattgc tttttgtaac acaattttac
     1861 cgctaacatc gagcatttta acaaatttca catcgttgct catccaatta atttgattgg
     1921 atgttggatt gggaaacaca ccaaaatcgg cttcaataga agaaattcca ttatcgccaa
     1981 tgcagaaatt aactattttt gaacttccaa atgtggctgc ttctactttt gtaatttgac
     2041 ctaaaatttc attcattggt cctttaatta ccaccattcc agagtcgcaa taactcattc
     2101 catcgctgta tgaatcaaag attttaaatg aatagcaacc ttcgctcaaa cacaatgaat
     2161 acgtatgcaa gccttcgtcg gtattgtaat aaaccggact tttatacaac gtatcgtttg
     2221 atgcattagt gatttgccaa ctggtttcac tggcataaca atcaacggtt acatctaaag
     2281 tcacagtatt gggattggtt accacatata aattggaagt cattttattg ttatcagcca
     2341 cttcatcgct tccaccgttc acgtttgtaa tggttgctga aaaagaatgc ccaccagctg
     2401 ttgctgttat tgtaggcaag gttatttctt gcgattgaaa catagttaag ttgccactcc
     2461 aattgtaggt ttgattttca actccatcca atccataagt gattgtagcc gatgtcaatg
     2521 ttgtttgtcc agtgtttacc aatgtaattt ttggttcaac tacagcatcg caaaccgttc
     2581 cgcttacatt ttttaaattc actaaacttc cgtctaactg aatggattct cctggctgat
     2641 aaccattcac caccaaagct cccgtattgg atgggtctaa ccaatatttt aactgtgttg
     2701 agctatcact ccccgttgga ttccaagaaa tgctaaaacg tccgtaataa tcaaacccat
     2761 cagtgttgga acatgatgct tgtccgcccc acaactgacc aataatgcgt ttatgttgat
     2821 tgaacaaagg tgaacctgat gaacctcctt ctgttgttgt atttcgatcc cactgcacgc
     2881 gccaagttcc gttaacatcg ctaacaactg agcccacttt tgtttgcgca ggaacagatt
     2941 gttgatcatc gaacgatatt tttttgatat ctccttttgg atggtgaatt ccaaaggtgc
     3001 ttgttgggtt ctcccctgtt ttatcccaac cagaaaaata ggcattgtag gctgcaggaa
     3061 ctgttcccga ttccaagccg cctgttatct caaccaagca aaaatcagag gtagaacggc
     3121 gtgctcttaa caccgcaccc gacatagaag tgaacgatgg tgatgaacca ggattgttgc
     3181 aatcaggtga ttcccagtta aatcgaaaca cccatgatgc agggttgctg taacaatggt
     3241 tggcagttaa cacataaggt tttccgtcat aagcagtgtt gtttaccaat gctccagtgc
     3301 aaaagccatt gctaccagaa accaacatta ctacgctgcg tttttgattt gcaaagggtg
     3361 taccttctgg acaatttaca tttttgtggc aagcaccaga ctgtccaaaa gctttttctt
     3421 gaaattcttc ggctgttcgg taaccgtgtg taaccgttgc aatggttaac tctccttgat
     3481 tatcattatt ttctggtgct acgtagtatt caacaataac tttacttcct ggaattaact
     3541 cggtaccaag ttgtccgttg taaatgtgtt tttgtgtaaa tttccctaaa atgaatgact
     3601 tgtttggatt gtaaacaaac agtgcatttc cttctggtat ttgcgttttt tcaagagtca
     3661 aattaaccgt tgttgcatcc acacattcta attgaagcaa ccacaattta ccaccattgg
     3721 gcaatgacaa ccaagtacct gaattattca acgttaaact ggtttcattg ttataaccaa
     3781 aacgccaagg tccctcaccc aaacgatcgt tttttgcatc ttctgcttgc aaagctttga
     3841 tatcgggttg taaaaattga actactggca aagtagattg taacgacgac caatctgtgc
     3901 ttttaccgcc atctccttga atttgtccga aaattaatgc tgtttgaacg atgaatatca
     3961 gtaataaaat ttgtttcata ggatgaattg tttgtttttt aaaaacgata tacgtaaaag
     4021 taaagtaaaa atttgaacct tcaaaaaaat ccattggatt taaaaagaaa attactgaaa
     4081 aagttataat tttacatcaa caaaatcgca tcatgcttca atttaacaaa gtattttatt
     4141 tacttacaac ttgcatttta ataagtgtaa gttcgtgtag caccataaaa aacgcaccat
     4201 cacaacgcca tggttcttgt tatttggaga atcgtttgcc ttatacctta tcatcagctc
     4261 ccaaaccact aattattgaa aatttagaca gcaatttaat tcattcgttt tctatttcaa
     4321 gtttaaacat tgccaatgga attggagttt tggacttact ttcagaatac gttcagcacc
     4381 gcaataaata caaaagcaac ccaacatttg aatcgcgttt gcaagccatc gaattgtttc
     4441 aaagaattga ttaccgcatt aatttagcat cgttagaaat ttcgtctatt gcctctgaaa
     4501 tggattgtga ggaagaaaaa attacgcaat tggctgattt tatgaaagaa aaagaaggtc
     4561 aagttgaaaa ccgactaaac gttgctgcta ttgctgttgg
//
LOCUS       DAOQVQ010000147         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20950_length_4586_cov_8.156698, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000147 DAOQVQ010000000
VERSION     DAOQVQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20950_length_4586_cov_8.156698"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKN22_05685"
     CDS             complement(<1..295)
                     /locus_tag="PKN22_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS42231.1"
                     /translation="MREKKGYSQEYMSDILNISQQTYSLTEKHPEKANLGRIKEIAKI
                     LEVKVSFLLNEDESFVLNTFNQQGHAISLVHSISNEDAYKSTINQMQSEIDFLR"
     gene            609..4535
                     /locus_tag="PKN22_05690"
     CDS             609..4535
                     /locus_tag="PKN22_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42232.1"
                     /translation="MAPQYHQVLPTGPGPSLPLPTSSSPEGYHGQAAQYAQNIQMDAS
                     GNILFFIVDDKIYDRNGECILSEDPWQFTSLFSYERLQNVNYFSQSTAMNGTASEILI
                     IPDKDDCNLFHIISSGAIHNSPSNFTPQNVGVAYGKLKISYDGNGNITPSSKISLQSF
                     SDANGTHVASMLSLEQIIGIQNLDFNDLVHQRSPTLAVVDNGNSYTVLYRNIQGLYRL
                     TIGNGNISYNNFITIPGSNQMPQNYEMEAVKLANGNIRVAAFNAINNNITGVQIVDIT
                     PLGTIVTGSEKTLQYDKNLFSSSGVRYFYGLEFSTDGNRLFVTHPTYNNNSTLDYWDI
                     SGTPVKTVLSNETEIGIGQIEMAKDGKMYIPRQNYLARISNINSTPTLNLTALSIPSY
                     SPVLGINSQQPPLRILQDQLDGSNVYDNVQPSTYHATSYNVQTSGVWDGVSNPIAPGS
                     SIINIEKELRIKAGVSVTFKNIMFQFAPGARLVIENGVSGGQGGKLLLDSTTLTNMPV
                     CGKEDMWLGVEVWGNSTVAQGTVLNSSQGVLRMYNNSKIEHAYVGVLLSKRNTDQAGV
                     VLLSSYDNNYNGGVAQVQNSEFLNCQYGMYFNTYTVGSNNLSTISGSTFKWENLFKKP
                     SVKPQNHIHLFKTSAVTITASNFLQETPAVYGGIDQYGKGIYSNNSEFKVYSGCTNIF
                     LPIGQDCAESNTVRSQFKNLVYGVYVVNSNSKPFVVLRNEFTNCSYGVYSIMAKNQTV
                     SRNKFHVAESTGQTCGLYVYRGTGYKIEENIFQEFDNPLIANGSGNSYGLIIYDSDTL
                     HNEVYKNTFSKLKIGAQAQGINGRKVGQNNDGTTGLQFICNEFKSPIYLTDIGVNGRI
                     DKEQGESGAISIQDARKRAARNVFSMYGEDPLVYPTHDIFMSAGSQYIEYTHLADNAH
                     TPDNYTILNVVAHTQTYIGADVYSDANTCPSKFGGKFIIFEPIALKNKSDSLSSKIDG
                     GNTAALMSAIETNPNSIQTQGLLLSKAPYLSDQVLESYFQSGASNAKVKNVLLANFGL
                     SDYLKNKLQASSRSNALKNDVLNFITSFSPRKQLLSELKQVHIAQNDMQRIAISSVLQ
                     DNTIANTYDSLEAILKQCVGLDFQESLLELYSWKQDQAKFDELLSQLSSQITAEKSQY
                     YQLIYAVDGGILSSTEIPHTFMRSLENLANQQNDVLVAASAQMLLDELINGEITPVIL
                     PLIATPSSRSSMIANEEQNTTALMGPAAIFPNPTSGEVTVLFSEIEASVAQVYLYDLS
                     GKEIMHQTFEKTTQGKLDLSNLKKGSYLIKVSVDGAPIKTNLLVVE"
BASE COUNT         1575 a          763 c          823 g         1425 t
ORIGIN      
        1 ttcttagaaa gtctatttca ctttgcattt gattgattgt acttttatac gcatcttcat
       61 tggatataga gtgaaccaat gaaatagcat gaccttgttg attaaacgta ttcaatacaa
      121 atgactcatc ttcatttagc aaaaaagaaa ccttgacttc taatatctta gcaatctctt
      181 taattctacc taagtttgct ttttcaggat gtttttcagt taaagaatat gtttgttgcg
      241 aaatgtttag tatatcagac atatattctt gactataacc ttttttttct ctcaaaaatc
      301 ggatgttttc tgaaatagac gcagaccttt ttaccataaa tacatgttta tttattacaa
      361 atatattaaa aatcgtattt ataaatacca attatattag taaaatacaa attttattgg
      421 taataacggt aggtaaatta aagtaatttc ggaaataatc ttgaaggtaa gtatttgtgt
      481 gcttttttta agatagtgaa taataaccta taattttaaa acttatgaaa aaatcattta
      541 gtcttttaat gtgtttgtta attttgtttt gcgcaaggaa tagcttgtct caaaacaatg
      601 catggatttt ggcaccacaa tatcatcaag tcttgccaac tggaccaggt ccttctctcc
      661 ccctccccac ttcctcttcc ccagaaggat atcatgggca agcggcacaa tatgctcaga
      721 acattcagat ggatgcatcg ggcaatattt tattttttat tgtagatgat aaaatttatg
      781 accgtaatgg ggagtgcatt ctttcggaag acccatggca gtttacgtct ctttttagtt
      841 atgaaaggtt gcaaaatgtt aattatttct cgcaatcaac agccatgaat ggtacggctt
      901 cagaaatact aattattccc gacaaagacg attgcaattt gtttcacatc attagctcgg
      961 gggcaattca caatagtcca agcaatttta caccacaaaa tgttggtgta gcttatggta
     1021 aattaaaaat ttcatacgat ggtaatggaa acattacacc atcatcaaaa ataagtcttc
     1081 aatcttttag tgatgccaac ggaactcacg ttgcctctat gctttcattg gagcaaatca
     1141 tcggaattca aaatttagat tttaatgatt tagtacatca acgttcaccc acacttgctg
     1201 tagttgataa tggcaattct tacacggtat tgtatagaaa catacaaggt ttgtatcgtt
     1261 taactattgg taatgggaac atttcttata ataatttcat tacaattcca ggttctaatc
     1321 aaatgcctca aaattatgaa atggaagctg ttaaattggc aaatggaaac atcagagtgg
     1381 ctgcatttaa tgctataaac aacaatatta ctggtgttca aattgtagat attactcctc
     1441 taggcacaat agttactggc tcagaaaaaa cgttgcagta cgacaaaaat ttattttcat
     1501 catccggagt tagatatttt tatgggttgg aattttctac agatggcaat cgtttatttg
     1561 taacgcatcc aacatacaat aacaatagca ctttagatta ttgggatatt tcaggcacac
     1621 cagttaaaac agttttgtca aatgaaactg aaataggaat tggacaaata gaaatggcaa
     1681 aagatggaaa aatgtacatt ccacgtcaaa attacttggc acgcatttca aatatcaaca
     1741 gcacaccaac tttaaacctt acagcgctta gtattccaag ttattcacct gttcttggta
     1801 ttaacagtca acaaccccct ctccgcattc ttcaagacca actagatggg agcaatgttt
     1861 atgacaatgt gcaaccaagc acttatcatg caacatcata taacgttcaa acaagtggtg
     1921 tttgggatgg agttagcaac ccaattgcac cgggttcatc aattatcaat atagagaaag
     1981 aattacgtat aaaagcagga gtgagtgtta cgtttaaaaa cataatgttt caatttgctc
     2041 cgggcgcacg cttagtaata gaaaatggtg tttcaggagg tcaaggtggt aagttattat
     2101 tagatagcac tacactcacc aatatgccag tttgtggtaa agaagatatg tggttgggcg
     2161 ttgaagtgtg gggaaattca acagttgctc aaggcactgt tcttaattca tcgcagggtg
     2221 tgctgagaat gtataacaat tctaaaatag aacatgcgta tgtaggtgtt ttattgtcaa
     2281 agcgaaatac ggatcaagcg ggagttgtat tgctaagttc gtatgataac aactataatg
     2341 gaggtgttgc tcaagtgcaa aacagtgagt ttctaaattg tcaatatggt atgtatttca
     2401 atacttatac tgtgggttcc aacaatttat ctacaatttc tggttctact tttaaatggg
     2461 aaaatttgtt taaaaaacca tctgtaaaac cacaaaacca cattcattta tttaaaacat
     2521 cggctgtaac aattaccgca tctaatttct tacaagaaac accagcagtt tatggtggca
     2581 tagaccagta tggtaaagga atttattcaa ataacagtga gtttaaggtt tatagcggtt
     2641 gcacaaacat ctttttgcct attggtcaag attgcgctga gtcaaacact gttcgctctc
     2701 aattcaaaaa tttagtgtat ggggtgtatg ttgttaattc caactcaaaa ccttttgttg
     2761 ttcttagaaa tgaatttaca aattgtagtt atggtgttta ttcaataatg gctaaaaacc
     2821 aaacggttag cagaaataaa tttcatgttg cagagtcaac agggcaaaca tgtggtttgt
     2881 atgtgtatcg aggaacaggt tataaaattg aggaaaatat tttccaagaa tttgataacc
     2941 cactcatagc aaacggaagt ggtaattctt atgggttaat catctatgat tcagacacgt
     3001 tacacaatga agtgtataaa aatactttta gcaaattaaa aattggtgca caagctcaag
     3061 gaatcaatgg aagaaaagtt ggtcaaaata atgacggtac aacaggacta cagtttatat
     3121 gcaacgagtt taaatctcca atttatttaa cagatatagg agtaaatggg agaatagata
     3181 aagagcaagg ggaaagtgga gcgatatcta tacaagatgc acgcaaaaga gcagcacgca
     3241 acgtattttc tatgtatggc gaagacccat tggtttaccc tacacatgat atttttatgt
     3301 cggccggtag tcaatacatc gaatatacgc atctggcaga taatgcacac acaccagata
     3361 actataccat cttaaatgtt gttgctcata cgcaaacata tattggtgct gatgtttact
     3421 cagatgcaaa tacgtgtcca tcaaaatttg gagggaagtt tattattttt gaacctattg
     3481 cattgaaaaa caaatcagat agtttgtcat caaaaataga tggaggtaac actgctgcgc
     3541 tgatgagtgc aatagaaacc aaccctaatt caattcaaac acaaggttta ttgctttcaa
     3601 aagcaccata tttaagtgat caggtgttgg agagttactt tcaatctggc gcatcaaatg
     3661 caaaagtgaa aaacgtattg ttagctaatt ttggtttatc tgactattta aaaaacaagt
     3721 tgcaagcctc ttcacgcagc aacgcactta aaaatgatgt attgaatttt attacgtcat
     3781 tctcaccaag aaaacaattg ttatccgaat taaaacaagt gcacattgct caaaacgata
     3841 tgcaacgcat tgctatttca agtgttttac aagacaacac aattgctaat acttatgaca
     3901 gcttagaagc aattttaaaa caatgcgtag gtttggattt tcaagaaagc ctattggaat
     3961 tgtatagttg gaaacaagat caagcaaaat ttgatgagtt attgagtcag ttaagttcac
     4021 aaatcacagc agaaaaaagt cagtattacc aattaattta tgcggtagat ggtggcatat
     4081 tgtcatctac agaaattccg catacattta tgcgttcatt ggaaaattta gccaatcaac
     4141 aaaacgatgt attagtggca gcttctgcac aaatgttact tgacgagctt ataaacggag
     4201 aaattactcc agtaatttta ccattaattg ctacaccatc tagcagaagt tcaatgattg
     4261 caaacgagga gcaaaacaca acagcgttga tgggaccagc ggctattttc cccaacccaa
     4321 catcgggcga agtaactgtt ttgttttcag aaatagaagc atcagtggcg caagtgtatt
     4381 tgtatgacct atcaggtaaa gaaatcatgc atcaaacatt tgaaaaaacc acccaaggca
     4441 aattggattt gtccaacttg aaaaagggaa gctacttaat taaagtaagc gttgatggag
     4501 cacccataaa aacaaacttg ttggtagtag aataaaacaa caaacactga taaattacgg
     4561 ctttcagaaa tggaagccgt tttttt
//
LOCUS       DAOQVQ010000148         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20978_length_4582_cov_4.310360, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000148 DAOQVQ010000000
VERSION     DAOQVQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20978_length_4582_cov_4.310360"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2482
                     /locus_tag="PKN22_05695"
     CDS             <1..2482
                     /locus_tag="PKN22_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS42233.1"
                     /translation="CDGSLTVVATGGSGTYTYTWKDNAGNVVGTNSATLSNICAGNYT
                     VEVTETGGGSGTQTIFTEDFESAGAGWNLNVPVATEGADPNFFVVNDNEGGVAPGGCG
                     VAGNGDATLHITSVFFPSGGAAYDAGGLCGFLFCPETHRRAESPTINTTGFTGLTLKF
                     DYIANGAIPNDQATVWYFDGATWNQLGTALSSGTGACAPQGIWTAYSQALPVVCENNP
                     NFKIAIRWDNNDDGAGTDPSVAINNIVIESTSGGGTGCPVTQAVTLTDPTAGATPSFT
                     FANFCAGATNGPTGISPTGGTFTFNPVPSDGATINATTGVISNGVAGTTYTVQYTTAG
                     ACAQSSTNNVTVFALPQSTISSTDASCGLNDGSATVNVTSGANPLSYSWNTSPVQTTQ
                     TANNLAGGSYTVTITDGNSCVNTNTVTVNQSSNITVTITPTQTSCTANTGTATANVTG
                     GTAPYSYSWNTSPVQTTQTAINLAVGNYTVTVTDNAGCIASQNVSISAANAPTASITT
                     SQNSTCFGSNNGTATVSATGGTAPYSYSWSPTGGNNATASNLSPNIYTVTVTDDAGCS
                     TTQQVTITEPAELIVTLSETDTDCGQSNGEITTNATGGTGTYSYVWTPNGETTSSITG
                     LNVGNYSVTVTDANGCLASATGTINTSTTFGLDATPAQSTIEAGGSVNLEIEVENGIT
                     IGTIDWTPTSGLSCTDCPNPTASPSQTTTYYVTVVDVNGCSATDSLIVTVKQPCGELY
                     VPTIFSPNGDQMNDLECVMGSCVVSIDFTIFDRWGEIVFHTKDRDNCWDGNFRGKPVQ
                     SGVYVYKLVATLENGDTVKESGNINVVR"
     gene            2507..3547
                     /locus_tag="PKN22_05700"
     CDS             2507..3547
                     /locus_tag="PKN22_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS42234.1"
                     /translation="MKRILIGTMLCLVTSTQAQDIHFSQMPYSPLTLNPGLAGANYDL
                     STSLNFRSQWNSVAEPFQTVAFSVDARLNAKNKRRDKKVHVAAGINFFNDRAGQAKVV
                     TNNANFHLATHIIMDYRHTLGLGIYGGWLQRTLDPNAGRWANQYDGMAYNPNYASGET
                     FNTLSFNNFDTGAGMVYTFYNGRESRIAANDSKMINVGFAAFHLNRPGYSFINKAQEK
                     MYIRFSAFANASFGIRNTHVLIEPGIYFHNQGNARELFFGLYGKYIIKGESHITNFVQ
                     QTMVGLGLFCRNQDALVLKAMFEWNGLGIGFAYDFNLFNSLIQLSKSRGGIEVALRWV
                     VPDLFKYNSRIK"
     gene            3597..4259
                     /gene="hxpB"
                     /locus_tag="PKN22_05705"
     CDS             3597..4259
                     /gene="hxpB"
                     /locus_tag="PKN22_05705"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HNS42235.1"
                     /translation="MNMNFNQFEAFIFDMDGVLIDSEPLWKIAMEQTFQEVGCSLTKK
                     DFQKTVGMRIDEVVSYWYHHTGWQNVSEKEVENRIVNRMIHLIEENGKPLPGVVDTLN
                     LLKAKGVKIGLATSSYAILIAAVLRTLDIQDAFHYVHSAEHEKYGKPHPAVYLTVAEK
                     LGVNPKSCLVIEDSLSGIIAGKAAKMTVVCIPEKTHHPEPKLIIADHQFESMEDLFVA
                     LQ"
     assembly_gap    4362..4371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1518 a          808 c          898 g         1348 t
ORIGIN      
        1 atgcgatggt tctttaactg tagttgctac aggtggaagt ggaacttata cttatacatg
       61 gaaagacaat gctggaaacg ttgttggaac taatagtgca actttgtcta acatttgtgc
      121 tggaaactat actgttgaag taacagaaac tggaggtgga agtggaacac aaactatctt
      181 tactgaagat tttgaatctg caggtgctgg ttggaatctt aatgttccag ttgctacaga
      241 aggagctgac cctaacttct ttgttgtaaa tgataacgaa ggtggtgttg ctccaggagg
      301 ctgtggtgtt gcaggaaatg gagacgctac tctacacatt acaagtgtgt tcttcccttc
      361 tggcggtgca gcttacgatg ctggtggatt gtgtggattt ttattttgtc cagaaacaca
      421 tcgtcgtgca gaatctccaa ctatcaacac aacaggattt acaggtttaa ctcttaaatt
      481 tgattatatt gcaaatggcg caattccaaa cgatcaagca actgtttggt attttgatgg
      541 tgcaacatgg aatcaattag gaactgctct ttcttctggt acaggtgcat gtgcgccaca
      601 aggtatttgg acagcctatt ctcaagcatt accagtggtt tgtgaaaaca atccaaactt
      661 taaaattgca atacgctggg acaataacga cgatggagct ggaacagatc cttctgtagc
      721 aatcaacaac atagtaattg aatctacttc tggaggagga acaggatgtc cggtaactca
      781 agcagtcaca ttaacagatc caacagcagg tgcaacacca agttttacgt ttgcaaactt
      841 ctgtgctggc gcaacaaatg gtccaacagg aatttcgcca acaggtggta cattcacatt
      901 taatccagtt ccttcagatg gtgctacaat caatgcaaca acaggtgtta tttctaatgg
      961 agttgctgga acaacatata cggttcaata tacaacagca ggtgcttgtg cacaaagtag
     1021 cacaaataat gttactgttt ttgctcttcc tcaatcgacc atctctagta cagacgcaag
     1081 ttgtggatta aacgatggtt cagctacagt caatgttact tcaggtgcaa atccactttc
     1141 ttatagttgg aatacatcgc cagtgcaaac tacacaaact gccaacaact tagcaggagg
     1201 cagttacaca gttacaataa ccgatggaaa tagctgtgta aatacgaata ctgtaactgt
     1261 taaccaaagt tcaaacatta cggttacaat aacaccaacg caaacatcat gtactgcaaa
     1321 tacaggaacg gctactgcaa atgttacagg tggaactgct ccttactctt atagttggaa
     1381 tacatcgcct gtgcaaacca cacaaactgc aataaattta gcagttggca attatactgt
     1441 aactgtaaca gataatgcag gatgtattgc atcacaaaat gtttccatca gtgctgcaaa
     1501 cgcaccaact gcatccatta ctacaagtca aaatagtact tgttttggtt ctaacaatgg
     1561 aactgctaca gtaagcgcaa caggaggaac tgctccttac tcttatagtt ggtcaccaac
     1621 aggaggtaat aatgcaacag cttcaaatct ttcacctaac atttacacgg taacagtaac
     1681 tgatgatgct ggttgttcaa ccacgcaaca agtaacgata acagaacctg cagaattaat
     1741 tgttacattg agtgaaacag atacagactg tgggcaaagt aacggtgaaa ttacaaccaa
     1801 tgcaacaggt ggtacaggaa cttacagtta tgtttggact ccaaatggag aaacaacttc
     1861 atcaataact ggattaaatg ttggaaatta ttcagtaacg gtaacagatg ctaacggttg
     1921 tttagcctca gcaactggga caattaatac atccacaaca tttggtttag atgctacacc
     1981 tgctcaatct acaatcgaag ctggaggaag tgtaaactta gagattgaag tggaaaacgg
     2041 aatcacaata ggaacaattg attggacgcc aacatctggt ttaagttgta cggattgccc
     2101 taatcctact gctagtccaa gtcaaacaac aacttattac gttactgtag ttgatgtaaa
     2161 tgggtgttcg gcaacagatt ctttaattgt aaccgttaaa caaccttgtg gagaattata
     2221 tgttcctacg attttctctc caaacggaga tcaaatgaac gatttagaat gtgtaatggg
     2281 ttcatgtgtt gtgtcaattg actttaccat ttttgataga tggggagaaa ttgtgtttca
     2341 caccaaagac agagacaact gttgggatgg taattttaga ggaaaaccag ttcagtcggg
     2401 ggtgtatgtc tataaattag ttgctacttt agaaaatggc gacacggtaa aagaatctgg
     2461 aaacatcaat gttgtccgct aagttgaact attaatacca caaaaaatga aaagaatatt
     2521 gataggaaca atgctttgtt tggttacatc aacacaagca caagacatcc atttttcaca
     2581 aatgccctat tctccattaa cgttaaatcc tggtttagct ggcgcaaatt atgacctttc
     2641 tacttcgtta aattttagga gtcaatggaa cagtgttgca gagccttttc aaacggtggc
     2701 tttttctgtt gatgcaagat tgaacgcaaa aaacaagaga agagataaaa aagtgcatgt
     2761 agctgcagga attaattttt ttaacgacag agctgggcaa gctaaagttg ttacaaacaa
     2821 tgcaaatttt catttagcca cacacatcat tatggattac cgccatactt taggtttggg
     2881 aatatacggt gggtggttgc aacgcacatt ggatccaaat gcaggaagat gggccaacca
     2941 atacgatgga atggcatata atccaaacta tgcgtcagga gaaacgttta acacgttatc
     3001 tttcaataat tttgacacag gagctggaat ggtttataca ttttataatg gtcgagaatc
     3061 gcgaattgca gcgaatgatt caaaaatgat aaatgttggt tttgcagcgt ttcatttgaa
     3121 caggcctggg tattcattca tcaataaagc acaagaaaaa atgtatatac gcttctctgc
     3181 gtttgcaaat gcttcttttg gaattagaaa tacccatgta ttgatagaac caggaattta
     3241 ttttcacaat caaggaaatg ctagagagtt gttttttggg ttgtatggaa aatacatcat
     3301 caaaggagag tctcacatta ccaattttgt gcaacaaaca atggttggtt tggggttatt
     3361 ctgccgaaat caagatgctt tggtattaaa agctatgttt gaatggaatg gactaggaat
     3421 tggttttgcg tatgatttca atttatttaa ctcattgatt caactttcca aatcaagagg
     3481 tggaattgaa gttgccttgc gttgggtagt tcctgattta ttcaaataca attccagaat
     3541 taaataaaaa attgaagctt aatttctaaa ttgtttacct ttacaaattc aggtaaatga
     3601 atatgaattt taatcaattt gaagctttta tttttgatat ggacggcgtt ttaatcgact
     3661 ctgaaccatt atggaaaata gcaatggaac aaacgtttca agaagttgga tgcagtttaa
     3721 ctaaaaaaga ttttcaaaaa accgtaggca tgcgcatcga cgaagtggtg agttattggt
     3781 accaccacac aggctggcaa aatgtttccg aaaaagaagt agaaaaccgc attgttaacc
     3841 gcatgataca cctcattgag gaaaacggaa aacctttgcc aggagttgtg gatacgttaa
     3901 atcttctcaa agcaaaaggc gtgaagatag gtttagcaac atcttcctat gccattttaa
     3961 ttgccgctgt attgagaaca ctcgatatac aagatgcctt tcattatgtg cattctgctg
     4021 agcatgaaaa atacggaaaa cctcatcctg cagtttatct cacagtagct gaaaaattag
     4081 gcgtaaatcc taaaagttgt ttggtgattg aagattcatt aagcggaatt attgccggaa
     4141 aagcagcaaa aatgacagtc gtttgcatac ccgaaaaaac acatcatccc gaacctaaat
     4201 tgataattgc cgaccatcaa tttgaatcta tggaagattt atttgttgca ctacagtagt
     4261 ttttaactta taaatggttt attgttaata tattaacaat ttgtaaaata agcaactttt
     4321 tactatttta gacgtctctg aactatatac taattattga tnnnnnnnnn naatatctaa
     4381 aaatacagat tgtgcaactg aataaattat tgatttatta tgaaaacaac ttgtaaactt
     4441 ttgtttgctt tcataacact gttaaataca ataggttatg ctcaacgctt aatcataaaa
     4501 ccaactacta acaatctatt actacaaaaa attgataaaa attttgtatc tcattccgaa
     4561 tacaatcaat atgctttagc ac
//
LOCUS       DAOQVQ010000149         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_20981_length_4582_cov_2.947427, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000149 DAOQVQ010000000
VERSION     DAOQVQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20981_length_4582_cov_2.947427"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /gene="rplO"
                     /locus_tag="PKN22_05710"
     CDS             complement(<1..330)
                     /gene="rplO"
                     /locus_tag="PKN22_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNS42236.1"
                     /translation="MSNLHNLTPAKGSVSSRRRIARGQGSGYGCTATRGNKGAKSRSG
                     YSKKIGFEGGQMPLQRRVPKFGFKNINRVEYKAINLDLIQALVERKNVTEISVEVLRE
                     NGLISNND"
     gene            complement(352..528)
                     /gene="rpmD"
                     /locus_tag="PKN22_05715"
     CDS             complement(352..528)
                     /gene="rpmD"
                     /locus_tag="PKN22_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNS42237.1"
                     /translation="MATIKIKQVRSAIKRPAVQKATVKALGFKKLNQVLEKEATPQIM
                     GMIRKVQHLVEVVE"
     gene            complement(544..1062)
                     /gene="rpsE"
                     /locus_tag="PKN22_05720"
     CDS             complement(544..1062)
                     /gene="rpsE"
                     /locus_tag="PKN22_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNS42238.1"
                     /translation="MAAQTMNRVKSSDIELKDRLVAVNRVTKVTKGGRTFSFSAIVVV
                     GDENGIVGHGLGKAKEVTEAITKAIDDAKKNLIKVPVLKGTIPHPQKGKFSGARVLIK
                     PASAGTGVIAGGAMRAVLESVGIHNVLAKSQGSSNPHNVVKATFDALGGLRDAATVAK
                     QRGITLDKVFNG"
     gene            complement(1065..1418)
                     /gene="rplR"
                     /locus_tag="PKN22_05725"
     CDS             complement(1065..1418)
                     /gene="rplR"
                     /locus_tag="PKN22_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNS42239.1"
                     /translation="MAFSKLNRRARIKRSIRKKISGTSMVPRLSVFRSNKQIYAQVID
                     DTNGKTLVSASSFKNKAVEKLNKTEQAAVVGKEVAEKAIKAGIQSVVFDRNGYLYHGR
                     VKSLANSAREGGLKF"
     gene            complement(1429..1986)
                     /gene="rplF"
                     /locus_tag="PKN22_05730"
     CDS             complement(1429..1986)
                     /gene="rplF"
                     /locus_tag="PKN22_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNS42240.1"
                     /translation="MSRIGKSPVNIPSGVDVKFDGNVITVKGSKGELSQVIDTDSVTL
                     SIEDGTITFSRATDAPSHRAKHGLYRALVYNMIVGVSEGYKSELEIVGVGFRATATGQ
                     QLELALGFSHPYVIELPKEIKVSADTQKGKAPVVTLESHDKQLLGQAVAKIRSLRKPE
                     PYKGKGIKFVGEDIRRKAGKSAGKK"
     gene            complement(2005..2403)
                     /gene="rpsH"
                     /locus_tag="PKN22_05735"
     CDS             complement(2005..2403)
                     /gene="rpsH"
                     /locus_tag="PKN22_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNS42241.1"
                     /translation="MNTDPIADFLTRIRNASSAGLKTVSIPASNTKKAITQILFDQGY
                     ITNYKFEDDSKQGIITIALKYNPSTRVPAITKIQRASRPGLRKYAGSQEIPSVQNGLG
                     IAVVSTSKGVITDKEARKLNVGGEVICYVY"
     gene            complement(2513..2827)
                     /gene="rpsN"
                     /locus_tag="PKN22_05740"
     CDS             complement(2513..2827)
                     /gene="rpsN"
                     /locus_tag="PKN22_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNS42242.1"
                     /translation="MAKESMKARERKREKLHARYAKKREELVKILKSSDQSDEIVNAK
                     WDAMMALQKLPANSAKNRQHNRCGLTGRPRGYMRQFGISRVTFREMALAGKIPGVKKA
                     SW"
     gene            complement(2831..3382)
                     /gene="rplE"
                     /locus_tag="PKN22_05745"
     CDS             complement(2831..3382)
                     /gene="rplE"
                     /locus_tag="PKN22_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNS42243.1"
                     /translation="MSYTPRLKEKYKAEIVAALTEKFGYKTTMRVPKLSKIVINQGLG
                     AAVADKKIIETAVEELSRITGQKAVATISKKDISNFKLRKGMPVGAMVTLRGDNMYDF
                     LDRFLSVAIPRTRDFRGVPATGFDGRGNFNYGVKEHIIFPEIDIDKVNRILGMDITFV
                     TTAQTDEEGKALLDAFGFPFIKK"
     gene            complement(3384..3701)
                     /gene="rplX"
                     /locus_tag="PKN22_05750"
     CDS             complement(3384..3701)
                     /gene="rplX"
                     /locus_tag="PKN22_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNS42244.1"
                     /translation="MQQKLHIKVGDTVKVISGTSRGREGKILSIDRNKSRAIVEGLNI
                     VKKHAKPSAQDPQGGIVEMEAGIHISNLMVVHNGQATKVGRKLNKDGKLVRYSKKSGE
                     EIK"
     gene            complement(3717..4085)
                     /gene="rplN"
                     /locus_tag="PKN22_05755"
     CDS             complement(3717..4085)
                     /gene="rplN"
                     /locus_tag="PKN22_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNS42245.1"
                     /translation="MIQSESRLTVADNTGAKEVLCIRVLGGTRRRYASVGDKIVVAVK
                     SAAPSGAVKKGAVAKAVIVRTKKEIRRIDGSYIRFDDNACVILNQAGDMRGTRIFGPV
                     ARELRERDYMKIVSLAPEVL"
     gene            complement(4095..4352)
                     /gene="rpsQ"
                     /locus_tag="PKN22_05760"
     CDS             complement(4095..4352)
                     /gene="rpsQ"
                     /locus_tag="PKN22_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNS42246.1"
                     /translation="MEERNLRKERVGVVTSDKMDKSIVVAVERKVKHPKYGKFVKKTT
                     KFVAHDEKNESNIGDTVRIMETRPLSKTKNWRLVEIVERAK"
     gene            complement(4380..4574)
                     /gene="rpmC"
                     /locus_tag="PKN22_05765"
     CDS             complement(4380..4574)
                     /gene="rpmC"
                     /locus_tag="PKN22_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNS42247.1"
                     /translation="MKQQEITKLSLDEVKSRIEGLVGQLAKIKVNHKVTPLENPSQIR
                     KMRREVAQLKTELTKREKQA"
BASE COUNT         1260 a          971 c          679 g         1672 t
ORIGIN      
        1 atcgttattt gaaatcaaac cgttttcacg taaaacttca acagaaattt cagtaacgtt
       61 tttacgttca accaacgctt gaattaaatc taaattaata gctttatatt caacacggtt
      121 gatatttttg aaaccaaatt taggtactcg tctttgaagt ggcatctgac ctccttcaaa
      181 accaattttc ttgctgtatc cagatcttga cttcgctcct ttgttaccac gtgtagcagt
      241 acatccgtat ccagaacctt gtcctcttgc gattcttctt ctgctgctta cagatccttt
      301 agcaggagtc aaattatgta aattgctcat gttaaatcaa ttttatttaa attattcaac
      361 aacttctact aagtgttgta cttttctaat cattcccatg atttgaggag ttgcttcttt
      421 ttcaagaact tggttcaact tcttaaatcc taacgctttt accgtagctt tttgtactgc
      481 tggtctttta atagcgcttc ttacttgctt aatttttatt gtagccatgt ttttgtctat
      541 ttattaacca ttaaatactt tatccaacgt aattccgcgt tgcttagcaa ctgtagcagc
      601 atctcttaat ccacccaatg catcaaatgt tgctttaaca acgttgtgcg ggttagaaga
      661 accttgagat tttgccaata cattgtgaat accaacactt tctaatactg cacgcattgc
      721 accacctgca ataactccag taccggcaga agctggtttg attaacacac gcgctccact
      781 aaatttccct ttttgagggt gaggaattgt tcctttcaat acaggaactt tgattagatt
      841 tttcttagca tcgtcgattg ctttagtgat agcctcagtt acctctttag cttttcctaa
      901 accgtgacca acaataccat tttcatcacc aactacaaca atagcagaaa aagagaatgt
      961 tctacctcct tttgttactt tcgttacgcg gtttacggct actaaacgat ctttcaattc
     1021 aatatccgaa gattttactc tgttcattgt ctgtgctgcc ataattaaaa ttttaaaccg
     1081 ccttctcttg ctgagttagc taatgattta actctaccgt gatacaaata tccattacga
     1141 tcaaaaacaa cgctttggat tcctgcctta attgcttttt cagctacttc tttaccaaca
     1201 actgctgctt gttcagtttt attcaacttt tcaacagctt tatttttgaa agatgatgct
     1261 gacaccaaag tttttccgtt tgtatcatca ataacttgag cataaatttg cttgttacta
     1321 cgaaaaactg ataatctagg caccattgaa gttccagaaa ttttctttct gatgcttctc
     1381 ttgattctag ctcttctgtt taatttacta aatgccataa tttaattttt attttttacc
     1441 tgcagatttt cctgcctttc ttctaatatc ttcacctacg aacttaatac cttttccttt
     1501 atatggttca ggcttacgaa gagaacgaat cttagcaacg gcttgaccca aaagttgttt
     1561 gtcgtgcgac tctaaagtca caactggtgc ctttcctttt tgggtatctg ctgatacttt
     1621 aatttctttc ggaagttcga taacataagg gtgagagaat cctaaagcta actccaactg
     1681 ttgaccagtt gctgtagcac ggaaacctac tcctacaatt tctaactccg atttgtaccc
     1741 ttccgatacg ccaacaatca tattataaac caaagcacgg tataaaccat gtttagctct
     1801 atgagaaggt gcgtcagttg cgcggctgaa agttattgtt ccatcttcga tagaaagagt
     1861 cacgctatct gtatcgataa cttgacttaa ttcacctttg gaacctttaa cagtaatcac
     1921 attaccgtca aactttacat ctactccact cgggatgttt actggggatt tacctatcct
     1981 tgacattctg ttatttgtta aagattaata tacgtagcaa attacttcac cacctacgtt
     2041 taattttctt gcttctttat ctgtaattac tcctttagat gtagaaacta cagcgattcc
     2101 taatccattt tgtacactag gaatttcttg tgatcctgcg tatttacgta aacctggacg
     2161 agaagctcgt tggatttttg taattgcagg tacacgagtt gaaggattgt atttcaaggc
     2221 aattgtaata attccttgtt ttgaatcatc ttcaaatttg taattggtaa tgtacccttg
     2281 atcaaaaaga atttgcgtaa ttgctttctt tgtgttagat gctgggatac taacggtttt
     2341 taaaccagca gaacttgcgt ttctgatacg cgttaaaaaa tctgctatcg gatctgtatt
     2401 catataaata aaaattcgac tatggttttt ggaaagcact gatttacaat gctttccaaa
     2461 cctttagtca gttaatacta cttaattggg gtgcaaatat aataaaatat ttttaccaac
     2521 tagctttttt cacaccagga atttttccag ctaatgccat ttcacggaaa gtaacacgtg
     2581 aaattccaaa ttggcgcatg tatcctctag ggcgaccagt taaaccacaa cggttgtgtt
     2641 gtctgttttt tgctgagttt gcaggcaatt tttgcaatgc catcatagca tcccattttg
     2701 catttacaat ctcatcagat tggtcactag atttaagaat ttttactaat tcttcacgtt
     2761 ttttagcata acgagcgtga agtttttctc ttttgcgctc acgcgctttc attgattctt
     2821 ttgccatctg ttattttttt ataaatggaa aaccaaatgc atctaataat gctttacctt
     2881 cttcatctgt ttgagcagtt gtaacgaaag taatatccat tcctaaaatg cgattcactt
     2941 tatcgatatc aatttcgggg aaaatgatat gttctttaac tccatagttg aagtttcctc
     3001 taccatcaaa accagtagct ggaacacctc taaagtcacg agtacgtgga attgctacag
     3061 agagaaaacg gtctaagaag tcatacatgt tatcacctct taaagtaacc attgccccaa
     3121 ctggcattcc ttttctcaat ttgaagttag agatatcctt tttagaaata gtagctacag
     3181 ctttttgacc tgtaattcta gacaattcct caacagctgt ttcaataatt ttcttatctg
     3241 ctacagcagc tcctaaccct tgattgataa caatcttaga taatttaggt acacgcattg
     3301 ttgttttgta cccaaatttt tcagttaaag cagcaacaat ttctgcttta tacttttcct
     3361 taagtcttgg tgtatagctc atattactta atctcctccc ctgacttttt tgaataacgt
     3421 actaattttc catctttgtt caatttacga ccaactttag tagcttgacc gttatgaaca
     3481 accataaggt ttgaaatatg tattcctgct tccatttcta caattccacc ttgaggatct
     3541 tgtgcacttg gtttagcatg ttttttaacg atgtttaaac cttcaacaat tgcacgactt
     3601 ttgtttctat caatcgaaag gatttttcct tcacgaccac gtgacgtacc gctaataacc
     3661 ttaacagtat cacccacttt aatatgtaat ttctgttgca taacttctac aatttattaa
     3721 agtacctcag gggcaagtga aacgattttc atataatctc tctcacgaag ctcacgagca
     3781 actggtccaa aaatacgggt tcctctcatg tcccccgcct gattcaagat aacgcaagcg
     3841 ttatcatcga aacgaatgta agaaccgtct atacgacgaa tttctttttt tgttcttaca
     3901 attactgcct tagctaccgc acctttttta acggcaccag agggcgctgc actcttaaca
     3961 gcaacaacga ttttatctcc aactgaagca taacgacgtc ttgtacctcc taaaactcgg
     4021 atacacaata cttcttttgc tcctgtgttg tctgctactg taagtcttga ttcactttgt
     4081 atcattacta aaaattattt agctcgttca actatttcta ctaatctcca attctttgtt
     4141 tttgacaaag gtcttgtttc cataatgcga actgtatctc caatattgga ttcatttttc
     4201 tcatcgtggg ctacaaattt agtagttttt ttaacaaact taccatactt agggtgtttt
     4261 acttttcttt caactgcaac aacaatagat ttgtccattt tgtcgctagt aactacacca
     4321 acacgttctt ttcttaaatt acgctcttcc atttcaatct ttttaaaatt tacaagctat
     4381 tatgcttgtt tctcacgttt tgtcaactca gttttcaatt gagccacctc tcttctcatc
     4441 ttgcgaattt gactagggtt ttccaacgga gttaccttat gatttacttt gatttttgct
     4501 aattgcccta ctaagccttc tatacggctt ttcacttcat ctaaagataa ttttgtgatt
     4561 tcttgctgtt tcatactaaa gg
//
LOCUS       DAOQVQ010000150         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21072_length_4568_cov_4.276313, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000150 DAOQVQ010000000
VERSION     DAOQVQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21072_length_4568_cov_4.276313"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /gene="rpsP"
                     /locus_tag="PKN22_05770"
     CDS             <1..392
                     /gene="rpsP"
                     /locus_tag="PKN22_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688702.1"
                     /note="binds to lower part of 30S body where it stabilizes
                     two domains; required for efficient assembly of 30S; in
                     Escherichia coli this protein has nuclease activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNS42248.1"
                     /translation="QVGAEMSNTARAILSYKGVLYKNHLIKGIAKGALTEAQVEEKFN
                     KWLAEKDAKITGKAEGLAKAKATQKAEALKAEAAVNEAKAKAIEAKNTPAPEVVEEEA
                     PVAEATEEATEATEAPAEESSEETSAE"
     gene            410..937
                     /gene="rimM"
                     /locus_tag="PKN22_05775"
     CDS             410..937
                     /gene="rimM"
                     /locus_tag="PKN22_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNS42249.1"
                     /translation="MNKADCFHLGYIAKLHGFKGEVSLFLDVTNPEDYRTLDALFIEL
                     NGQLTPFFITSLKLKNKGFASIKLEGIDSEEDARMLLRKKVYLPASILPELGDTNFYD
                     HEIVDFAVEDTNHGAIGKVIQVIDLAANPLIQIEKAGKEILLPLQEGVIQKVDRKNKT
                     LIVTAPPGLVELYLG"
     gene            941..1645
                     /locus_tag="PKN22_05780"
     CDS             941..1645
                     /locus_tag="PKN22_05780"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNS42250.1"
                     /translation="MSVFHFKYFSIQQSNSALKVGTDAMLLGAHLADNTYHSILDIGT
                     GTGVLALMAKQKNPLAKVTAIDIDTASLIDCEFNFNHSPWASDFKIIQQNFLEFKPTT
                     TYDCIVCNPPYYENGYLSNNSRTNTAKHVKEFTIEELFKKVNELLHPKGDFWVIFPFS
                     SKENWLSIAQSFGLYPKSQITLNGKKDNPNRLIVQFCKTPQTVIYKTLTIRNSDNSYT
                     KEYIELTKNFHNKSLI"
     gene            1804..3066
                     /locus_tag="PKN22_05785"
     CDS             1804..3066
                     /locus_tag="PKN22_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42251.1"
                     /translation="MKKFTLSLASLLLAFSGFSQAVQDSIKMGASYANEMYLKLEDGS
                     RSTHAANSWHVGFATSAYSVAVVSNPGLPSPGMGQAGISISVYPNGDASTFASVDTTG
                     FSTWPKLYNDSLDYEVGAFNQSTNGTQFDYGWGQYNSINHQITGDSLYIVKIGSDCYK
                     FKIVSKAAGKYTFNYALISDNTEGTQVEINGSTDYATKNFVFFNFADGQVKDLELAGW
                     DLWATKHYDWYGTTPNQVVTGILVNPKWDVATVNVGSGNQASHNDYSSANFVNKKNNI
                     GSTYKSLNMQTFAWEVTDSKVYYLQNAAGDVWKLYPTSFSGSSTGETKFMKEHIAYAG
                     VENVATQFVDVYPNPVENTATVVFEGKGATEIIVRNQMGQVVVAQSLASTSGITQHQL
                     DLSNLTNGMYFVQINQNGSSIVKNIVKK"
     gene            3138..>4568
                     /locus_tag="PKN22_05790"
     CDS             3138..>4568
                     /locus_tag="PKN22_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNS42252.1"
                     /translation="MLLRYFNFFVLVFVVSTAFSQQVTFKSTEDSTTLKVNATIYEEN
                     KKVANLQSANGTLDFSTLKDGQFIEVAAFGHDVFSFTKAGQIPSTIYLKKTITSFDEM
                     IITGQLQHTTVSNCVQNVRVINSKKIEQMGAQNLTQLFKNELNIQLSNDAILGSGMDL
                     QGISGENVKILVDGVPIIGKMNGTVDLNQINIQNVEKIEIIEGPLSVNYGSDALAGTI
                     NIITKKNQAALFSGKVYGYYESNGTYNTQLNLSSKFKNTLLTADFNRNYFDGWRADEK
                     PFHIEKVRIADSLRNKLYKPREQYFGGFKLHQRLNKDKQEHGLFLTIDARLFKENIEN
                     RGTPSKPYFIKAFDDYYTTFRSDNSLSINGRMTDKWSVNSTNAYNFFKRVKNTYFNDL
                     TTLEQTLAGASEQDTSMFDLILSRTSFIYNHSKSWSLELGYDVNYESSRGKRIGKGTQ
                     AIGDYALFITSEYTAWNKLTIKPGLRA"
BASE COUNT         1616 a          797 c          788 g         1367 t
ORIGIN      
        1 tacaagttgg agctgaaatg tctaacactg caagagcaat tttatcttac aaaggtgttt
       61 tgtacaaaaa ccacttgatt aaaggtattg ctaaaggtgc gttgacagaa gcacaagttg
      121 aagaaaaatt caacaaatgg ttggcggaga aagatgctaa aattacagga aaagcagaag
      181 gtttagcaaa agctaaagca actcaaaaag cagaagcatt gaaagcagaa gcagctgtaa
      241 acgaagcaaa agctaaagca attgaagcta aaaacactcc tgctccagaa gtagttgaag
      301 aagaagctcc tgttgcagaa gctacagaag aagcaactga ggcaactgaa gctccagcag
      361 aagaatcttc tgaagaaaca tcagctgagt aattaaaaat tgacattgaa tgaataaagc
      421 agattgcttt catttaggtt atattgcgaa acttcacgga tttaaaggtg aagtttcgct
      481 ttttttagac gtaaccaacc ccgaagatta cagaacctta gacgccttgt ttattgaatt
      541 aaacggtcaa ctgacacctt ttttcataac ttctttaaaa cttaaaaaca aaggatttgc
      601 ttctattaag ctagaaggaa tagactccga agaagatgca cgcatgttat tgcgcaaaaa
      661 ggtttattta cccgcttcta tcctacccga attgggcgac accaatttct atgaccacga
      721 aattgttgat ttcgccgttg aagataccaa ccacggggct atcggtaaag taattcaagt
      781 aatagattta gctgccaatc cgcttattca aattgaaaaa gccggtaaag aaattttgct
      841 tccattgcaa gagggcgtta tccaaaaagt ggatagaaaa aacaaaacgc ttatagttac
      901 agctcctcca ggattagtgg aattgtacct aggttagtac atgagtgttt ttcactttaa
      961 atatttttcc atacaacaat ccaatagtgc tttaaaagtt ggaaccgatg ccatgttatt
     1021 gggtgctcac cttgcggata atacttacca ttccatttta gacattggca caggaacagg
     1081 cgtactagca ttaatggcca aacaaaaaaa tccgttagcc aaagtaaccg ctattgatat
     1141 tgatacagct tctttgatag attgtgaatt caacttcaac cattcacctt gggcaagcga
     1201 ctttaaaatt attcagcaaa attttttaga atttaaacca acaaccacct atgattgcat
     1261 tgtgtgcaac ccaccttatt atgaaaacgg ctacttatca aacaactcac gtaccaacac
     1321 tgctaaacac gtaaaagaat ttacaattga agaattattt aaaaaagtca acgaactgct
     1381 tcatcccaaa ggtgattttt gggtgatttt tccgttctca tctaaagaaa actggctatc
     1441 aattgcccaa tcatttggtt tatatcctaa atctcaaata acattaaacg gaaaaaaaga
     1501 caatcccaat cggttgattg tacaattctg caaaacacca caaactgtta tttataaaac
     1561 actaactatc cgtaattcag ataattccta cacaaaagaa tacattgaac tcactaaaaa
     1621 ctttcacaat aaatcactga tttgatattt tttttcatgt ctgacatttg tatgacacta
     1681 aaggcgactt gattgtctaa ttttgttgcg aaattttaaa aatacctttt tcttggtatt
     1741 gttaaaaatt cttaaaggct atagttttgc cttaatttaa aattaatctt aataaaaata
     1801 agtatgaaaa aatttacact cagtttagct tctctacttt tggcatttag tggcttttca
     1861 caagctgttc aagattcaat caaaatggga gcttcttatg caaatgaaat gtatttgaaa
     1921 ttagaagatg gttcaagatc aacacacgct gcaaacagtt ggcatgttgg ctttgcaaca
     1981 agtgcctatt cagtagcagt tgtttcaaat ccaggcttac cttctccagg aatggggcaa
     2041 gctggtatca gtatttctgt atacccaaat ggtgatgcgt ctacatttgc ttctgttgat
     2101 acaacaggat tctctacgtg gccaaaatta tacaacgact cattggatta tgaagttggg
     2161 gctttcaacc aaagtacaaa tggaactcaa tttgattatg gttggggaca atacaattca
     2221 attaatcacc aaatcacagg agactctctt tatattgtga aaattggaag tgattgttat
     2281 aaatttaaaa ttgttagcaa agctgctggt aaatacactt ttaactatgc gttaatttct
     2341 gacaatacag aaggtacaca agtagaaatt aatggttcta ctgattatgc aactaaaaat
     2401 ttcgtattct ttaactttgc tgatgggcaa gtaaaagatt tagaattagc aggttgggac
     2461 ttgtgggcaa ctaagcacta cgattggtat ggaactacac ctaaccaagt tgtaacagga
     2521 attttagtaa accctaaatg ggatgttgca acagttaatg taggttctgg aaaccaagca
     2581 tctcacaatg attacagtag tgctaatttt gtaaacaaga aaaacaacat tggttctacc
     2641 tacaaatcat tgaacatgca aacatttgct tgggaagtaa ctgattctaa agtatattac
     2701 ttacaaaatg ctgcaggcga tgtttggaaa ttgtatccta cttcttttag cggttcatca
     2761 acaggtgaaa caaaattcat gaaagagcat attgcttatg caggtgttga aaatgttgct
     2821 actcaatttg ttgatgtata tccaaaccca gtagaaaaca ctgctacagt tgtttttgaa
     2881 ggaaaagggg caactgaaat tattgttaga aaccaaatgg gacaagtagt agttgctcaa
     2941 agcttagctt caacttctgg aattacacaa caccaattgg atttgtctaa tttaacaaat
     3001 ggtatgtact tcgtacaaat caaccaaaat ggttcttcga ttgtaaaaaa tattgtaaaa
     3061 aaataaataa acagtcatag ccaaggaggg tgaattgctt ttgattcacc ttccttattt
     3121 aattaaaaaa cacaccaatg ttactccgtt atttcaattt tttcgtttta gtttttgttg
     3181 tttcaactgc attttcacaa caagttactt ttaaatcaac tgaagattca acaaccttaa
     3241 aagtcaatgc aaccatttat gaggaaaata aaaaagtagc taatctgcaa tctgcaaatg
     3301 gaacgcttga tttttctaca ttaaaagacg gacaatttat tgaagttgcg gcatttggac
     3361 acgatgtgtt ttcgtttaca aaagcaggtc aaattccctc taccatttac ctaaaaaaaa
     3421 ccatcacttc atttgatgaa atgattatta caggtcaatt acaacacact actgttagca
     3481 attgtgtgca aaatgtaagg gtcatcaact ctaaaaaaat tgagcaaatg ggtgctcaaa
     3541 acctaacaca actatttaaa aacgaattaa atattcaact ttctaacgat gctattcttg
     3601 gttctggaat ggacttgcaa ggtatttcgg gagaaaatgt aaaaatttta gtggatggtg
     3661 ttccaatcat tggaaaaatg aatggaacgg ttgatttgaa ccaaattaac atccaaaatg
     3721 tagaaaaaat agaaattata gaaggtccgt tgtcggttaa ctacggttcc gacgctcttg
     3781 ccggcacaat caacattatt actaaaaaaa atcaagctgc cttattttct ggtaaagttt
     3841 atggatacta tgaaagtaat ggcacctaca acacacaact aaacctttca tcaaaattta
     3901 aaaacacact actcactgct gattttaacc gtaattattt tgacggttgg agagcagatg
     3961 aaaaaccttt tcatattgag aaagtacgca ttgccgattc acttcgcaat aaattataca
     4021 aacctagaga acagtatttt ggaggcttta aacttcatca acgtttaaac aaagacaaac
     4081 aagaacatgg gttgttttta accatagatg cccgtttgtt taaggaaaat attgaaaaca
     4141 gaggaacgcc ttcaaaaccc tatttcataa aagcatttga tgattattac actacttttc
     4201 gttctgataa tagcctctca attaatggta gaatgaccga taaatggtcg gtaaactcaa
     4261 ccaatgccta caattttttt aaacgtgtta aaaacaccta ttttaacgat ttaacaacgt
     4321 tggagcaaac tttggctgga gcgtcagagc aggatacttc catgtttgat ttaattttaa
     4381 gtagaaccag tttcatttac aaccattcaa aaagttggtc gcttgaattg ggatacgacg
     4441 taaattatga aagctctcgt ggaaaacgca ttggtaaagg aactcaagca attggcgatt
     4501 atgccttgtt tatcacctcg gaatacaccg catggaataa attaaccatc aaaccgggtt
     4561 taagagca
//
LOCUS       DAOQVQ010000151         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21261_length_4537_cov_4.197903, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000151 DAOQVQ010000000
VERSION     DAOQVQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21261_length_4537_cov_4.197903"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..2107)
                     /locus_tag="PKN22_05795"
     CDS             complement(41..2107)
                     /locus_tag="PKN22_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS42253.1"
                     /translation="MKTIIAIVALALSYSAFAQNIDFKAANFKDDKEGLKKATDAIKT
                     GDELFKLANEALFATQSPGLNFKKALIQYQVAQKFNAQNALNNFKIGVCYMNSSDPGK
                     GIPFIKKAAELDKACDPFMSYYLGYALQLEGKFSEALKLYDEFETGYKKADNFSKFVA
                     MHKNECKNAQAAVANPVRAWVDNLSELNSEADDFAPSISTDGSEIILSSNRPNGKTPN
                     EAGEYDFDIYTSSSQDLKWAKPQAIKGAVNSAADDVSNNLNFDGTKMLMHREENGQMD
                     IYESELKGLNWSDPKKAHFQISSPRANEIYGAYSNDGWSIYFARDNATRSTGFDIMFS
                     QMQSKIAKDYMAASMLAEVNTGFNDGPIYMHIDGETMYIASQGRASLGGYDIFVSKKV
                     QGKWTTPQNMGYPINTPYDDFFFAPTANGKFAYIASNRDGGKGGYDLYKVTFWGEDKK
                     PSLQTEDYLLASLIMPIKDNKIEATVDVNKKSFTVFKGKTIDAITKKAVEAQIEITDN
                     ATGKVIETFTTNSATGKFIITLASGKNYGIIVKADGYLFHSENFDIPKGAADNLVDKT
                     IELKNIKIGSTIALRNIFFDTGKSTLRPESNSELDRLVKLLNDVPSLKIEISGHTDNT
                     GSAKLNEQLSKDRAEAVVVYLKGKGITASRLTSEGYGASKPIASNATEDGRQENRRTE
                     FKITGN"
     gene            complement(2132..3229)
                     /locus_tag="PKN22_05800"
     CDS             complement(2132..3229)
                     /locus_tag="PKN22_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42254.1"
                     /translation="MIKHKLLLCVGLGLFSTYSHAQIEELTPAEKKYRDSITALNMQN
                     ATIANSQNAYNDGIKFFSEGKYQQARDRFKQSLSFDPNFTAASYNLGVTENELGSYAD
                     ASKTLSQLIEKDPSYSKAYFQRGRAYQGLNEYLKAENDYDKSIALDPSNPRAYYNYGT
                     LRFLQQDYKTAITYFTKAIELDKNFAFAYNDRGSCYRMLGDYTKALADYQEAERKNPN
                     LAFVLNNIGTTTSKMKNFQGAMAAFNRAISIDANFYLAYNNRGVLELENNKMDDAKRD
                     FEKTLEINPKYAPAASNLSGLYFKQKDYKKALEYANKAVELDKNYAGAYVNRANAKEM
                     LRDMIGACEDWEKANELGDGNGKIYYSSNCQ"
     gene            3351..>4537
                     /gene="pcaF"
                     /locus_tag="PKN22_05805"
     CDS             3351..>4537
                     /gene="pcaF"
                     /locus_tag="PKN22_05805"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNS42255.1"
                     /translation="MSQEVYLVEGVRTAIGRFGGTLAGVRTDDLAAIAIKGLMDKMPN
                     LDPLLIEDVILGCANQAGEDNRNVARMAGLIAGLPYQVAGETVNRLCSSGLASVMNAT
                     RAIKDGCGDIYIAGGVENMTRGPWVISKASTPFGRDSEMHDTSFGWRFINPKLHELYG
                     TDSMGNTAENLAELYNINREDQDKFAFWSQQKTAQAVKNGIFDAEIIPVEIPQRKKDP
                     IIFKTDEFAKADTTLEGLGQLRPAFKKDGTVTAGNASGLNDGAAALLLASEKAVKEQN
                     LKPIARIVAGAVAGVEPRIMGIGPVYATEKVLKRTGLTLDQMDVIELNEAFAAQVLAC
                     TRKMGLADNDPRINPNGGAISLGHPLGMTGARTTLTAAKELQRTNKQYALVTMCIGVG
                     QGYA"
BASE COUNT         1331 a          834 c          843 g         1529 t
ORIGIN      
        1 tctgtatcgg ttatttagga ctagtcaata tttctacaca tcaatttccc gtaatcttaa
       61 actcggtgcg tctgttttct tgtcgtccat cttctgtagc attagatgca attggttttg
      121 atgcaccata accttcagat gttaaacggg atgctgtaat tcctttccct ttaaggtaaa
      181 ccacaacagc ttctgcacgg tcttttgata actgctcgtt caattttgca gaaccagtat
      241 tgtcggtatg accagaaatc tcaattttta aacttggaac atcgttcaat aatttcacta
      301 aacgatccaa ctccgaattg gattctgggc gcaatgtaga tttgcccgta tcaaagaaga
      361 tattgcgcaa agcaatggtg cttccaattt tgatgttttt caactcaatt gttttatcca
      421 ccaagttgtc ggcagcacct tttggaatgt caaagttttc agaatgaaat aaataaccat
      481 ctgcttttac aatgattccg tagtttttac cagaagccaa agtgatgata aatttacctg
      541 ttgcactatt cgttgtaaat gtttcaatta ctttacctgt tgcattgtct gtaatttcaa
      601 tttgtgcttc taccgctttt ttggtgatgg catcaattgt ttttccttta aatacggtaa
      661 acgatttttt gtttacatcc actgtagctt caattttatt gtcttttatc ggcataatta
      721 aagaagccaa taaataatct tctgtttgaa ggcttggttt tttatcttca ccccaaaatg
      781 ttactttgta aaggtcatat cctcctttgc ccccatctct attagaagca atataggcaa
      841 atttcccatt agccgttgga gcaaagaaaa aatcgtcata cggtgtgttg atgggatatc
      901 ccatattttg tggagtagtc catttacctt gaactttctt agaaacaaaa atatcgtagc
      961 ctcccaacga agctcttcct tgcgaagcaa tatacatcgt ttctccatca atgtgcatgt
     1021 aaattgggcc atcattaaaa ccagtattta cttctgctaa cattgaagca gccatgtaat
     1081 cttttgcaat tttactttgc atttgcgaga acatgatgtc aaaacctgta cttctggtgg
     1141 cattgtctct ggcaaaataa atgctccaac catcgttaga gtaagctcca tagatttcat
     1201 tggctcttgg agatgaaatt tgaaaatgag cttttttagg atccgaccaa ttcaagcctt
     1261 ttaactcact ttcataaata tccatttgtc cattttcttc acgatgcatc aacatctttg
     1321 ttccatcaaa atttaagttg ttggaaacgt catccgcagc tgagttcaca gctcctttaa
     1381 ttgcttgtgg ttttgcccat tttaggtctt gcgaagatga agtgtaaata tcaaaatcat
     1441 attctcccgc ttcattgggt gttttaccat ttggacgatt agaggataga ataatttcac
     1501 taccatctgt tgaaatggaa ggcgcaaaat catctgcttc gctgtttaat tcagataagt
     1561 tatctaccca tgcacgtact ggattagcaa ctgccgcttg tgcatttttg cattcgtttt
     1621 tgtgcatggc aacaaacttg ctgaaattat ctgctttttt gtaaccagtt tcaaattcat
     1681 cgtatagttt caatgcttct gaaaatttac cttccaattg caatgcatat cctaaataat
     1741 aactcataaa tgggtcacat gctttgtcta attcagctgc tttttttata aacggaattc
     1801 ccttacctgg atctgatgag ttcatgtagc aaaccccaat tttaaaatta ttgagtgcat
     1861 tttgtgcatt gaatttttga gcaacttggt attgaattaa cgcttttttg aaattcaaac
     1921 ccggactttg agttgcaaac aatgcctcat ttgccaattt aaaaagttca tcaccagttt
     1981 tgatggcatc ggttgctttt tttagtcctt ctttatcgtc tttgaaattt gccgctttaa
     2041 aatctatgtt ttgtgcaaat gcggagtagc tcaatgcaag tgctacgatt gctataattg
     2101 ttttcataat tttcttgttg attaatacga cttattggca attggaagaa tagtaaattt
     2161 taccatttcc gtctcctaat tcattggctt tttcccaatc ttcgcatgct ccaatcatat
     2221 cgcgaagcat ttctttggca ttggcacggt tcacataagc acctgcataa tttttatcca
     2281 attcaaccgc tttattggca tattccaatg cttttttata gtctttttgc ttgaaataca
     2341 aaccgctaag attagacgca gctggtgcat atttagggtt aatctccaat gttttttcaa
     2401 aatcgcgttt agcatcgtcc attttattgt tttccaattc taaaacccca cggttattgt
     2461 aagctaagta aaaatttgca tcaattgaaa tggcacggtt aaaagctgcc attgctcctt
     2521 gaaaattttt cattttactg gttgttgtgc caatgttgtt caacacaaag gcaagatttg
     2581 ggttttttcg ttcagcttct tgataatcgg ccaatgcttt tgtgtaatct cccaacatac
     2641 ggtaacaact tccacggtcg ttatatgcaa atgcaaagtt tttatccaat tcgatggctt
     2701 ttgtaaaata agtaatggct gttttgtaat cttgttgcaa gaaacgcaag gtgccatagt
     2761 tataatatgc tctaggatta gatggatcca atgcaattga tttatcataa tcattttctg
     2821 ccttcaaata ttcattcaaa ccttgatagg cacgtccacg ctgaaaataa gctttggaat
     2881 agctaggatc tttttcaatc aattgactca atgttttaga agcatctgca tagctaccta
     2941 actcattttc agtaacacct aaattatagg aagcagctgt gaagttaggg tcaaaactaa
     3001 gcgattgttt aaaccgatca cgagcttgtt ggtattttcc ttccgaaaaa aactttatcc
     3061 catcgttata cgcattttga ctgttggcaa ttgttgcgtt ttgcatgttt aaagccgtaa
     3121 tggaatccct gtattttttt tcggctggtg ttagttcttc tatttgtgca tgggaataag
     3181 ttgaaaacaa ccctaagcca acacaaagca gtaatttatg ttttatcatt gtacagaaat
     3241 ttaatgaggc ttaaatgtaa aaaatatttt agtaaatcag gaattaagtt ttcaacaaga
     3301 tgaaaaaatt gtattttagc cgttggaata ttaacaattt aaaataaaaa atgtcacaag
     3361 aagtttattt agttgaagga gttcgcacag caattggaag atttggcgga acgttggcag
     3421 gagttagaac agatgattta gcagcaattg ccatcaaagg tttaatggat aaaatgccga
     3481 atttagatcc gcttttaatt gaagatgtta ttttaggatg tgccaaccaa gctggtgaag
     3541 ataaccgaaa tgtggcaaga atggcaggct taattgcagg tttgccatat caagttgctg
     3601 gagaaaccgt taaccgtttg tgttcttctg gattggcttc tgtaatgaac gcaacacgtg
     3661 ccattaaaga tggatgtggt gatatttaca ttgcaggagg agtggaaaat atgacacgtg
     3721 gaccgtgggt gatttctaaa gcatcaacac cttttggacg cgattctgaa atgcacgata
     3781 cttcgtttgg atggcgtttt attaacccta aattgcacga actttacgga acagattcta
     3841 tgggaaatac cgctgagaat ttagctgaat tgtataatat taaccgtgaa gaccaagata
     3901 aatttgcttt ttggtcgcaa caaaaaacag cccaagctgt aaaaaatggc atttttgatg
     3961 ctgaaattat tccagttgaa attcctcaac gcaaaaaaga tcctatcatt tttaaaacgg
     4021 atgaatttgc taaagcggat actacgttgg aaggattggg acaattacga cctgcattta
     4081 aaaaagatgg aactgtaacc gcaggaaatg cttctggatt aaacgacgga gctgctgcct
     4141 tgcttttggc atctgaaaaa gcagtgaaag aacaaaattt aaaaccaatc gcacgcattg
     4201 tggcaggcgc agtggcaggt gttgaaccac gaattatggg aattggacct gtgtatgcaa
     4261 ccgaaaaagt gttgaaaaga acagggttaa cacttgatca aatggatgtg atagaactta
     4321 acgaagcttt tgcagcgcaa gttttagcat gtacacgtaa aatgggatta gccgataacg
     4381 accctcgtat caacccaaat ggtggagcta tttcgttggg acacccacta ggaatgaccg
     4441 gtgcacgcac aactttaacg gctgctaaag aattgcaaag aactaataaa caatatgcgt
     4501 tggtaaccat gtgtattggt gttggacaag gttatgc
//
LOCUS       DAOQVQ010000152         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21408_length_4513_cov_3.694482, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000152 DAOQVQ010000000
VERSION     DAOQVQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21408_length_4513_cov_3.694482"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKN22_05810"
     CDS             <1..690
                     /locus_tag="PKN22_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42256.1"
                     /translation="QAYIHSIIDDTSIVAINDSLIKYLELCRGLEFQKQLLNVYSTTQ
                     NQHKFDSLIVKLPISRDLMSYYRVIYDLNKYNDWSVAVQEDTTIIARLNNLRHSSDPE
                     VENITSMLVSTLEKQPVSLIVMPLSSSSRSLITSMPTEENILNEESENILLYPNPAKD
                     ELFIKFINKSDAKETIVRFYDLTGKEVLKTQVNEQQNSIINLNGLNKGIYLVKLFSDG
                     QLQETHKLVIQ"
     gene            complement(783..1298)
                     /gene="rfaE2"
                     /locus_tag="PKN22_05815"
     CDS             complement(783..1298)
                     /gene="rfaE2"
                     /locus_tag="PKN22_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNS42257.1"
                     /translation="MTRLEHLSNKIVSLEEAKRRMNIWRLAGLKVTFTNGCFDILHKG
                     HVTYLTQAADLGKRLVVGLNTDASVRRQGKSPERPINDEAARATVLASLSAVDLVVLF
                     DEDTPLELIEALQPNIVAKGSDYDAQERDTTSKKYIVGAHLMDKTGGQVVAIPLVEGF
                     STTNIISKIKK"
     gene            complement(1270..2310)
                     /locus_tag="PKN22_05820"
     CDS             complement(1270..2310)
                     /locus_tag="PKN22_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNS42258.1"
                     /translation="MSKDTILKVVKTVLPLAIGIYLFWLFFSKMTDEHIASFKRALRE
                     ANYFWVFLAMVLEFVSLWSRAHRWKYLLEPLGYSTPWKNRYHALMVGYLANYTLPRAG
                     EPTRSAMLFRSDGVPFAKSFGTIIAERAVDVAMLGLVVLITMALGANDLKTIFASIEE
                     QLGGKSAETTGFDFKQLIYFIGGGLVLIGILVYIFNAKIREKLKQFIIGLLSGVFAIF
                     KTKNPWMFIVHTFIIWSSWVLMFVVPFYSLKETADVPFTGMLIGFVVGSLGMSLTNGG
                     IGVYPLLVGMVVAFYLQDTHPADATGIGNALGMIIWLGNTIIMILLGLISLVLLPKNY
                     SKEDDTSRTPQQ"
     gene            complement(2312..2662)
                     /locus_tag="PKN22_05825"
     CDS             complement(2312..2662)
                     /locus_tag="PKN22_05825"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNS42259.1"
                     /translation="MLVQVVKSKIHRVRVTQADLNYIGSITIDEALMDAANFIEGERV
                     QIVNLNNGERLETYIIKGERNSGTICLNGPAARKVAVNDVVIIIGYGMMDFEEAKTFK
                     PSFVFPNEQTNLLD"
     gene            2776..3393
                     /locus_tag="PKN22_05830"
     CDS             2776..3393
                     /locus_tag="PKN22_05830"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNS42260.1"
                     /translation="MLIEINPYNIDDRIIRQIIDELKAGGVIIFPTDTVYAMGCDLYN
                     KKGLQELARLKDVKLEKSNFSIICKDFSDISNYVKHISRPIFKVLKQNLPGPFTFILP
                     ATNEIPKLFGNNKKEVGIRIPDNAIVQRIVEELGHPIAVTSLHNDDDLIQDYFVDPYA
                     IYERYEEDVAIIIDGGYGQLEASTIVNCTTDEPEIVRQGVGVLDI"
     gene            3448..>4513
                     /gene="guaA"
                     /locus_tag="PKN22_05835"
     CDS             3448..>4513
                     /gene="guaA"
                     /locus_tag="PKN22_05835"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNS42261.1"
                     /translation="MQDKILIIDFGSQYTQLIARRIREINVYCEIFPWDKIPAITSDV
                     KGVILSGSPFSVSDANAPTPDLSQIKGKLPLLGICFGAQYLAAKFGGEVLPSTIREYG
                     RANLAKVNTNCVLTRGMTTGSQVWMSHGDTIKVVPSNYKIIASTADVEVAGYEIEGEQ
                     TYGIQFHPEVYHSLEGALLLRNFIVDVCKCDQSWTPLSFVETTVAELKEKIKDDKVIL
                     ALSGGVDSSVAAMLLHKAIGSNLHCIFVNHGLLRKNEYDEVLDSYKHMGLNVKGVDAS
                     ERFFAALKGLTDPELKRKAIGKTFIDVFDEESKLIEGATWLGQGTIYPDVIESVSVNG
                     GPSKTIKSHHNVGGLPDYMKL"
BASE COUNT         1474 a          835 c          806 g         1398 t
ORIGIN      
        1 caagcttata ttcattccat cattgacgat acaagcatcg tagctataaa tgacagtctg
       61 attaaatacc ttgaattgtg tagaggttta gaatttcaaa agcaattatt aaacgtatat
      121 agcactactc aaaatcaaca taaatttgat tcattaattg ttaaattacc tatttctaga
      181 gatttaatgt cttattaccg tgtaatatac gatttgaata aatataatga ttggagtgta
      241 gcagttcaag aagatactac aattatagct cgattaaata atttacgtca ttcttctgat
      301 ccagaagtgg aaaacattac ttcaatgctt gtttcaactt tagaaaaaca acctgtttca
      361 ttaattgtaa tgcctcttag tagttcttct agaagcttaa ttacatcaat gccaacagag
      421 gaaaatatat taaatgaaga aagtgaaaac attttattat atccaaatcc agcaaaagat
      481 gagttgttta ttaaattcat aaataaatca gatgcaaaag aaacgattgt tcgtttttat
      541 gatttaactg gtaaagaagt gttgaaaaca caggtaaatg aacaacaaaa ttcaataatc
      601 aacctcaatg gattgaataa aggaatttat ttagtaaaat tattctctga cggacaattg
      661 caagaaacgc acaagttagt cattcaataa cttcatcatg aaaaatgtaa gaggctgttc
      721 aacttttcgg acagcctctt ttttatttca atcttcagat caggataatt ccttatcctc
      781 acttattttt taatcttgct aataatgttg gtggttgaaa aaccttcaac tagtggtatg
      841 gcaacaactt gacctcccgt cttgtccatt aaatgggcgc caacaatgta tttttttgaa
      901 gttgtatcac gctcttgtgc atcgtaatcg cttcctttgg caacaatatt gggttgcaat
      961 gcctcaatga gttctagtgg cgtgtcttcg tcaaataaca ctactaaatc cacagcgctt
     1021 aaactggcca atacagtagc tcttgcagct tcatcgttga ttggtcgctc gggtgatttt
     1081 ccttgtcttc ttaccgaagc atcggtgttt aaacccacca ccaatcgttt gcctaaatct
     1141 gccgcttggg ttaaataggt tacatgccct ttgtgcaaaa tatcaaaaca accattggta
     1201 aaggttactt ttaagcctgc aagtcgccaa atattcattc tacgcttagc ttcttctagc
     1261 gaaacaattt tattgctgag gtgttcgaga cgtgtcatct tctttggaat agtttttagg
     1321 taataaaact aatgaaatta atcctaaaag tatcatgatt atggtatttc ccaaccaaat
     1381 tatcatgcct aatgcattgc ctattcccgt tgcatctgct ggatgtgtgt cttgcaagta
     1441 aaatgctaca accattccaa caagcaacgg ataaacgcca attccgccat tggttaacga
     1501 catacccaac gaacctacaa caaatccgat gagcatccct gtaaatggca catcagctgt
     1561 ttctttgaga gaatagaatg gaaccacaaa catcaatacc cagctgctcc aaattataaa
     1621 ggtgtggaca atgaacatcc acggattttt ggttttaaaa atggcaaaaa caccactcag
     1681 caaacctata atgaattgct ttagtttttc gcggatttta gcattgaaaa tgtacaccaa
     1741 aataccaatc aacaccaaac cgccgccgat aaaataaatc aattgtttaa aatcaaaacc
     1801 agttgtttca gccgattttc ctcccaattg ttcttcaata gatgcaaaaa tggttttcaa
     1861 atcattggca cccaacgcca tagtgattaa tactaccaat cctagcatgg caacatccac
     1921 cgcacgttct gctattatgg ttccaaacga ttttgcaaaa ggaacaccat ctgagcgaaa
     1981 aagcattgcc gaacgagttg gttcaccagc acgaggaagg gtgtaattgg ccaagtaacc
     2041 taccatcaat gcgtggtaac ggtttttcca aggtgtggaa tagcccaagg gttccaacaa
     2101 atatttccaa cgatgagcac gcgaccacaa agaaacaaat tccaatacca ttgccaagaa
     2161 aacccaaaaa taattggctt ctcgcaatgc acgtttgaaa ctggcaatgt gttcatcggt
     2221 cattttagaa aaaaacaacc aaaaaagata gataccaatt gctaaaggta aaactgtttt
     2281 tacaaccttt aaaatggtat cttttgacat gttaatctaa caaattggtt tgttcgttag
     2341 ggaaaacaaa cgatggtttg aacgtttttg cttcttcaaa atccatcatg ccataaccaa
     2401 taataattac aacatcgttt accgccactt ttctggcagc cggaccgttc aaacaaattg
     2461 ttccagaatt tcgctctcct tttatgatat acgtttccaa acgttcaccg ttgtttaggt
     2521 taacaatttg aacacgttct ccttctataa aatttgccgc atccatcaac gcttcatcaa
     2581 tggtaatact accgatatag tttaaatcgg cttgcgttac acgcacacgg tggattttgg
     2641 acttaactac ttgtactaac atatttacta tattccggcg caaagttaga taaatatacc
     2701 tatatatgcg atttaatttg taaatttgtt ttattcagaa ttccaacaac agttattttc
     2761 aaatagtaag ttttcatgtt aatagaaatc aacccatata acatcgatga tcgtatcatt
     2821 cgccaaataa ttgatgaatt aaaagctgga ggtgttatca tttttccaac cgataccgtt
     2881 tatgctatgg gctgtgattt gtataacaaa aagggcttgc aagaattggc gcgactcaaa
     2941 gacgtgaaat tggaaaaatc caacttttca atcatttgca aggattttag cgatatttct
     3001 aattatgtaa aacacatttc tcgcccaatt tttaaagtat tgaaacaaaa tttaccggga
     3061 ccttttacct ttattttgcc tgcaaccaat gaaattccca aattgtttgg caataataaa
     3121 aaagaagtgg gtattcgtat tccagataat gcaattgtgc agcgaatagt agaagaatta
     3181 ggtcatccaa ttgctgtaac gtctttgcac aacgacgacg atttgattca agattatttt
     3241 gttgatcctt atgctattta cgaacgttat gaggaagatg tggcaataat tattgatggg
     3301 ggatatggtc agctagaggc ttcaacaatt gttaattgca ccaccgacga accagaaatt
     3361 gtacgtcaag gggttggtgt gttggatatt taataaaatt ccatccagaa ttagcaatat
     3421 cacatcctat tttcttactt ttgcaccatg caagacaaga ttttaataat tgatttcggt
     3481 tctcaatata cacagttgat tgccagaaga atacgtgaga taaatgttta ttgtgagatt
     3541 tttccttggg ataaaattcc ggcaataaca agcgatgtaa aaggtgtaat tttatcagga
     3601 agtccatttt ctgtgagcga tgccaatgca ccaactccag atttaagtca aattaaagga
     3661 aaattacctt tgttgggcat ctgctttgga gcacaatatt tagctgcaaa atttgggggt
     3721 gaagtattgc catctaccat tcgcgaatac ggtagagcta atttggcaaa agtaaacacc
     3781 aattgtgtgt taacaagagg aatgacgact ggttcgcaag tatggatgtc gcacggtgac
     3841 accataaaag ttgttccatc caattataaa atcattgcaa gcacggcaga tgttgaagtg
     3901 gctggttatg aaattgaagg cgagcaaacg tatggtatcc aattccaccc agaggtttac
     3961 cactcattgg aaggagcttt gttgttgcgc aattttattg tggatgtttg taaatgcgac
     4021 caatcgtgga caccactttc atttgttgaa acaaccgttg ccgaacttaa agaaaaaatt
     4081 aaagacgata aagttatttt agcattatct ggtggggttg attcttctgt tgctgccatg
     4141 ttgttgcaca aagccattgg aagcaacttg cattgtattt tcgtgaacca cggtttgttg
     4201 cgtaaaaacg aatacgatga agtgttggat tcttacaaac acatgggatt aaacgtgaaa
     4261 ggtgttgacg cttctgaacg tttctttgct gctttgaaag gattgacaga tccagaatta
     4321 aaaagaaaag ccatcggtaa aacatttatt gatgtgtttg atgaggaatc aaaattaatt
     4381 gaaggcgcaa cttggttggg tcaaggaact atttaccctg atgtcatcga atcggtttct
     4441 gtaaatggag gtccttcaaa aactattaaa tcgcaccaca acgtaggtgg tttacccgat
     4501 tatatgaagt tga
//
LOCUS       DAOQVQ010000153         4495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21524_length_4495_cov_3.231757, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000153 DAOQVQ010000000
VERSION     DAOQVQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21524_length_4495_cov_3.231757"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKN22_05840"
     CDS             complement(<1..213)
                     /locus_tag="PKN22_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42262.1"
                     /translation="MNYALMREYTDSTQLSLIVLTYTILNFSLHMFNNYFDFKEDTIT
                     NQTLKKEGRKMTFYLAIISFLISLLLV"
     gene            complement(301..1116)
                     /locus_tag="PKN22_05845"
     CDS             complement(301..1116)
                     /locus_tag="PKN22_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42263.1"
                     /translation="MKLIACLSIVITVLLIQFIDSLKINANNVFIVLGQAHFFMAYWY
                     KIKRQNPSTTKYLMLIISGFFIFVSYYFLKYEHLLIILTSIYFVIHFSLDVFHLTNYK
                     ATIVTFIKTATPLFIVYLSFLKLGNFSELLFIYGFPLSFLLFFTLHKSEKWYFFFFTG
                     FATFLYTFSLLERFNIYLGSALFHQYFMPIIIAHYLIWYLYYFDKLKKQEFLKKYVVD
                     VLLFNGIIGLAFFFYFYNKESVFKYFFRLDYFLIWTWLHYIWTLRKSDFQLLK"
     gene            complement(1116..2006)
                     /locus_tag="PKN22_05850"
     CDS             complement(1116..2006)
                     /locus_tag="PKN22_05850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS42264.1"
                     /translation="MKLSVLLFSYNQQKFITQAIDSVLSQKTSFEFQLIIADDGSTDG
                     TVEIIKRYENENPTKIVFLDSHKRGIIENIFRMEKHIAGDYIAILDGDDYWSSEQKIQ
                     QQIDFLENNTNYIACYHDAAIISTVDSSSNYFDTANFYSQQHHYKENTLPADVVNRLI
                     IPTGSVVFRKIGLTKILEHKDLLGDYYSLDWKIYCLLIADSKFYYFNQVWSTYRNHPQ
                     GISKSNKTQFHLSHIHFLTQLLKHQYYKYLKLSIYNSLASEYTILLNDEATKNKKQLF
                     RQYLLNEMKRMWWFRKEVFK"
     gene            2068..2916
                     /locus_tag="PKN22_05855"
     CDS             2068..2916
                     /locus_tag="PKN22_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNS42265.1"
                     /translation="MDASGKIAIQIEDVKYNFNVRDVIFGFGQNEVLSQNNSDIAYGQ
                     PWYIKGFTEVDFLSNEEFIILKNEITQSIQTIVEGVLKRSLVDFTLEDYHKFIIKNDD
                     HLAVVSQTRDLFEENVTINLPFIIRKIEDILHLNLTDIVPSTNYKMHLIIRINRPNSN
                     DFNPPHKDIYEFYDEYKLTPKMINCWIPVAGVDINSSLPIAPASHLISENKIERTFTG
                     GYFEGNKYRVRIIKKWDGSNKLLRSKVNYGQILLFSPYLVHGVAINNTTQTRVALELR
                     LFEETR"
     gene            complement(2909..3517)
                     /locus_tag="PKN22_05860"
     CDS             complement(2909..3517)
                     /locus_tag="PKN22_05860"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNS42266.1"
                     /translation="MKITLIGASGLAKDLIDIFRYENYIREVALFDNISDIQEKKLYN
                     THDILTSIDELQKRFKEVSPFFITAIGSPRKRKEISQQLETIGGYNFSYICSQSLVSQ
                     YSTISERGVIIQMSCQISSDVVIEDGVFLNVRCMIGHDVKIGKYTTLSPDVKLLGNAV
                     IGENCVLATGVTVMPNVKIGNNVKIGMNKLVTEDVPDGTMLI"
     gene            complement(3536..4264)
                     /locus_tag="PKN22_05865"
     CDS             complement(3536..4264)
                     /locus_tag="PKN22_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS42267.1"
                     /translation="MDEDGNLVHEHWALKDVSLAIKKGESVGIIGPNGSGKSTLLKIL
                     AGVTKPTHGEVKIRGKVASILDIGAGFHPELSGRENVYLNGQIHGFSKKEIASKFDEI
                     VAFSGIEKFIDEPVKNYSNGMYLRLAFSIMAHLDFDVYLLDEVLSVGDAEFREKILEL
                     INTWNNKKTLVVVTHDFFQIHSIIDRVVAFNSTTYQEVEHQPQPFELLIANYMKEHAI
                     TASEFDFKLEAPDFELDIQYKKKY"
BASE COUNT         1595 a          696 c          654 g         1550 t
ORIGIN      
        1 aacaagtaat aatgaaatta aaaaagaaat gattgcaagg taaaatgtca tttttcttcc
       61 ttcttttttt aacgtttgat tcgtaattgt atcctcctta aaatcgaaat aattattaaa
      121 catgtgcaat gagaaattca aaattgtata tgtaagtaca attaagctta attgagtaga
      181 atctgtatat tcacgcatta aggcgtaatt catgaaaaaa aagatcatta aaaaatgaat
      241 tttaagccta tttatcacta cccaactttt taaaatgaaa ttcctcatat tttaattata
      301 ttatttaagc aactggaaat cagattttct caatgtccat atataatgca accatgtcca
      361 aattaaaaaa taatctaaac ggaaaaaata tttaaaaaca gactccttgt tataaaaata
      421 aaagaaaaat gctaaaccaa taataccatt aaacaataat acatccacaa catatttttt
      481 taaaaactct tgttttttga gtttatcaaa ataatagaga taccaaatta aataatgagc
      541 aataataata ggcataaaat attggtgaaa aagtgcgctt cctaaataaa tattgaatcg
      601 ttcaagtaaa gaaaaagtat acaaaaatgt tgcaaatcct gtgaagaaaa agaagtacca
      661 tttttctgat ttatgaagcg taaaaaagag taagaaactt aaaggaaacc cataaataaa
      721 cagaagctca ctaaaattgc ctagttttaa aaatgataaa tagactataa ataatggcgt
      781 ggctgttttt ataaatgtta caattgtcgc tttgtaatta gttaaatgaa aaacatctag
      841 actaaaatga ataacaaaat agatggatgt taagataatt agcaagtgtt cgtattttag
      901 gaaataatag ctaacaaaaa tgaaaaaacc agaaattatt aacatcaaat attttgtggt
      961 tgagggattt tgacgtttaa ttttatacca atatgccata aaaaaatgtg cttgtcccaa
     1021 aacaataaac acattgtttg catttatctt taaactgtct ataaattgga tgagcaagac
     1081 agttatcaca atggataaac atgctattaa tttcatcact taaaaacttc ttttctaaac
     1141 caccacattc gtttcatttc atttaaaaga tattgtcgaa acaattgctt tttatttttg
     1201 gtagcttcat cgtttaataa tatagtatat tcacttgcta atgagttata tatacttaat
     1261 tttaggtact tataatactg atgtttcaat aattgagtca aaaaatggat gtgcgacaaa
     1321 tgaaattgtg ttttgttgct ttttgaaata ccttgaggat gatttcgata cgttgaccaa
     1381 acttggttga aataataaaa tttagaatcg gcaatgagta aacaataaat tttccaatcc
     1441 aacgaataat agtcgcccaa caaatctttg tgttccaaaa tttttgtcaa accaattttt
     1501 ctaaatacaa cagagccagt agggataatt aatcgattca caacatctgc aggcaatgta
     1561 ttttctttat aatgatgttg ttgtgaataa aagtttgccg tatcaaaata attggacgaa
     1621 ctatctaccg tagatataat tgctgcatcg tggtaacaag caatatagtt ggtattgttt
     1681 tctaaaaaat caatttgttg ctgtattttc tgttcactcg accaataatc atcaccatct
     1741 aaaatggcta tgtaatctcc agcaatgtgt ttttccatcc taaagatatt ctcaataatt
     1801 cctcgtttat gcgaatctag aaaaacaatt ttagtcggat tttcattttc atagcgttta
     1861 attatctcaa ccgtaccgtc tgttgaacca tcatcagcaa taattaattg aaactcaaaa
     1921 gatgtttttt gcgacaatac actatctatt gcttgagtaa taaatttttg ttgattgtat
     1981 gaaaaaagca gtacactgag tttcatttac ttgattttat tagtcaaata taatcaaaag
     2041 attatatatt tataaaaaat atagtgtatg gatgcatcag gcaaaattgc tattcaaatt
     2101 gaagatgtaa aatataattt taatgtaaga gatgtaattt ttggatttgg acagaacgaa
     2161 gtactatctc aaaataattc cgatatagct tatggtcaac cgtggtatat taaaggattt
     2221 acagaagttg actttttgtc aaatgaagaa tttataatat taaaaaatga aattacgcaa
     2281 agtattcaaa caattgttga aggtgtttta aaacgttcat tagtcgactt cactttagaa
     2341 gattaccata aatttattat taaaaatgat gatcatttag cagttgtatc acaaactcga
     2401 gatttatttg aggagaatgt aacaattaat ttgcctttca ttataagaaa aattgaagac
     2461 atcctccatc tcaatttgac agatattgta ccaagcacca attataaaat gcaccttatt
     2521 attcgaataa atcgaccaaa ttcaaatgat tttaatccac ctcacaaaga tatttatgag
     2581 ttttatgatg aatataaatt aactcctaaa atgattaatt gctggattcc agtagcagga
     2641 gttgatatca attcatcctt gcctatagcc cctgctagtc atcttattag tgaaaataaa
     2701 attgagcgta catttacggg tggttatttt gagggcaaca aatatcgtgt aagaataata
     2761 aaaaaatggg atggcagtaa taaacttctt cgttcaaaag taaactacgg tcaaatattg
     2821 ttattttcac cctatttagt acatggtgtt gctatcaata atactacaca aacaagagta
     2881 gcattagaat tacgtttgtt tgaagaaact agataagcat tgttccatct ggtacatctt
     2941 cagttactag tttattcatc ccaattttaa cattgtttcc aattttaaca tttggcatga
     3001 ctgtaacacc agttgcaagc acacaatttt caccaataac agcatttccc aacaatttta
     3061 catcgggtga taatgtggtg tatttaccaa tttttacatc gtgccctatc atgcaacgaa
     3121 catttaaaaa cacgccgtct tcaattacaa catcgcttga aatttgacaa ctcatttgaa
     3181 tgatgactcc tctttctgaa atagttgaat attgacttac caacgactgc gaacagatgt
     3241 atgaaaaatt atatcctcca attgtttcca actgctggct gatttctttg cgttttcttg
     3301 gtgacccaat tgccgtgata aaaaatggag aaacttcttt aaatcttttt tgaagttcat
     3361 caatggaagt aagaatgtcg tgagtattgt atagtttttt ttcttgaata tccgaaatat
     3421 tgtcgaaaag tgctacttca cggatgtaat tctcatatcg aaatatgtcg ataagatctt
     3481 ttgctaatcc agatgcacca attaatgtaa ttttcatttt ttttatttta agttgttagt
     3541 atttcttttt atactgaata tccaattcaa aatcaggagc ttctaattta aaatcaaatt
     3601 cactggctgt tattgcatgt tctttcatgt aatttgcaat taatagttca aagggttgtg
     3661 gttggtgttc cacttcttga tacgttgtgc tgttaaacgc aactacacgg tcaattattg
     3721 agtgaatttg aaaaaaatca tgcgtaacca ccaccaatgt ttttttattg ttccatgtat
     3781 ttatcaattc caaaattttc tctcgaaact ccgcatcgcc cacactcaac acctcatcca
     3841 acaaatacac atcaaaatcc aagtgtgcca tgatggaaaa tgccaaacgc aaatacatgc
     3901 cgttggagta atttttaact ggctcatcta tgaatttttc tatgcccgaa aatgcaacaa
     3961 tttcatcaaa tttggaagca atttcttttt tggaaaagcc gtggatttgt ccgtttaaat
     4021 acacattttc tcgtccactt aattcgggat gaaaaccagc accaatgtcc aaaatactgg
     4081 ccaccttgcc tctaattttc acttctccgt gggtgggttt ggtgactcct gccaaaattt
     4141 tgagcaaagt gcttttgcca ctgccgtttg gaccaataat accaacactt tcgccttttt
     4201 tgattgctaa tgaaacatcc ttcaatgccc aatgttcatg cactaaattt ccatcttcat
     4261 ccatttgcgg gtggcgaagt ggataaacct tcgaaacgtt cgaaatttgt atagcaatat
     4321 cggttttcaa attggtgttt taggtactaa aatagtgttt ttttagtaga ttgagtaaaa
     4381 aaatgaaatg ttgttgtaga catttaactg tttttgatgg cattcaacga atactattaa
     4441 aagaattagg aaattaattt gttttgttca tacacttttt ttgaatgaaa ccagt
//
LOCUS       DAOQVQ010000154         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21771_length_4461_cov_3.557422, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000154 DAOQVQ010000000
VERSION     DAOQVQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21771_length_4461_cov_3.557422"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKN22_05870"
     CDS             complement(<1..497)
                     /locus_tag="PKN22_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS42268.1"
                     /translation="MSQEIELVNCLIIGSGPAGYTAAIYAARADMKPVLYQGMQPGGQ
                     LTTTNEVENFPGYPEGISGPEMMVQLEQQAKRFEADIRFGFITKVDFSGPIHKAWTES
                     GQEIHAKTVIISTGASAKYLGLENEQKYLALGGGVSACAVCDGYFFRGQETVIVGAGD
                     SACEEA"
     gene            complement(673..1878)
                     /locus_tag="PKN22_05875"
     CDS             complement(673..1878)
                     /locus_tag="PKN22_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS42269.1"
                     /translation="MKKAFALYFNSFKGLSRESWMLSLVMLINRTGGMVIPFLTVYLT
                     SVLGFQTDEAGLAMSFFGIGSLLGSVIGGWLTDKVGAFKVQFFSLFFAAPLFVFLAYI
                     KQPHEFYVSIFLLSTIYESFRPANSAAITSYAKKGNLTRSFSLNRLAINLGFSMGPAL
                     GGMLATFSYSWLFFGNGFAVFATSIVFFVYFFKRKERNVSENQHEESTTSVQHSPYKD
                     KWFLIFSFYCFLFSCIFFQLISIMPLFYSQSLHFTQFEIGLLLGFNGLFVVLTEMFVV
                     TLFEKRFNIYTILIIGCVALGISFLWLLFDTSLVSCFIAMAFLSVSEILVLPFMSTVA
                     AKRSSVKNRGAYMGLNGLSISAAFVVMPYLATLFIEHFSFERLWYINIFILLITAFGF
                     RFIRSKMPL"
     gene            complement(1943..2016)
                     /locus_tag="PKN22_05880"
     tRNA            complement(1943..2016)
                     /locus_tag="PKN22_05880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1980..1982),aa:Asn,seq:gtt)
     gene            complement(2110..2562)
                     /locus_tag="PKN22_05885"
     CDS             complement(2110..2562)
                     /locus_tag="PKN22_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNS42270.1"
                     /translation="MATVFETKLIGNIGKDATVKSMSSGMIAINFPVAHNKNWKDKKT
                     GEQRTNTTWVNCTIWKREGANMRVVDYLKRGTLVELAGTPFAKAYTNEDGSIRTEIRL
                     NVFKTNILRSAPSVSNPNDGDMYSEDDSEDDDDFTSNLSDFSLNDDDF"
     gene            2702..3604
                     /locus_tag="PKN22_05890"
     CDS             2702..3604
                     /locus_tag="PKN22_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42271.1"
                     /translation="MKVLTIHFSQSGQLTEILTNFTQPFNGVEMEHQFIVPKNKFPFP
                     WDVKTFFETMPESVLEIPIELEAINYNYSKYDLVIIGYQPWFLSPSLPITSLLKDEQF
                     KHIIKDTPIITVIGSRNMWLNSQESVKKLIQQAGGKLVGNVALADKSDNLIGVITILH
                     WMLKGEKTRKWGIFPKPGVSDEDIENVKLFGEFAAQALKKNDWNNFQNQVIQNDGVVV
                     RTNILFIEQKAKRLFIIWANLILKQAKKGKRSFWVNAFKYYLIFVLFVVSPILLILYR
                     VLIWPFTFKKVEARKKYFQQIAIK"
     gene            3692..4249
                     /locus_tag="PKN22_05895"
     CDS             3692..4249
                     /locus_tag="PKN22_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42272.1"
                     /translation="MILKDVYITRIASFLPNNPVSNDEMEEYLGVINQQASKSRRIVL
                     RNNGIKNRFYALTKGGKSTHSNSEMAALSIKKLFQNPAQELATLELISCATSSPEQLM
                     PSHAVMVHGHLPETRSVEVVSPSGVCCAGMHALKYAYMSVKLGQVKRAVSSASERLSP
                     SLKADKFELEVKKTKRVGRKSIRFF"
     gene            4221..>4461
                     /locus_tag="PKN22_05900"
     CDS             4221..>4461
                     /locus_tag="PKN22_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42273.1"
                     /translation="MEENPYVSFDKDFLRWMLSDGAGAFLVEDKPNENSNSLRIDWIE
                     GFSFANEIEPCMYMGAEKREDGSLISYLDFEPQESI"
BASE COUNT         1536 a          749 c          766 g         1410 t
ORIGIN      
        1 gcttcttcgc aagcagagtc gccagcacct acaatcactg tttcttgtcc gcggaaaaaa
       61 tatccgtcac acacagcaca tgccgaaaca cctccaccaa gtgccaagta tttttgttcg
      121 ttttctaaac ctaaatattt tgccgaagct cctgttgaaa taatcacagt tttagcgtga
      181 atttcttgtc cgctttctgt ccaagctttg tgtattggtc ccgaaaaatc aacttttgta
      241 ataaatccaa atcgaatatc ggcttcaaaa cgtttggctt gttgttccaa ttgtaccatc
      301 atttctggtc cagaaattcc ttcaggataa ccagggaaat tttctacttc attggttgtt
      361 gtaagctgtc cacctggttg cattccttga tacaacactg gtttcatatc tgcacgtgca
      421 gcgtaaatgg cagcagtgta tcctgctggt ccagatccta taattaagca attgactaat
      481 tctatttctt gactcatttt tgtatgtttt tctatgattt taatgtaaaa gacaaaaata
      541 atgaaaaaag cacagttgag aatgactaaa gtcacaaatg aaaattaagt tttaaacaat
      601 gttgaaaact atagagtaga gattttttta atcagttttg aagtattgtc agagtttcgt
      661 tgactgttaa gattacaacg gcattttgct gcgaatgaat ctaaatccaa aagcggtaat
      721 taaaagaata aaaatattta tataccacaa tctttcaaat gaaaaatgct caataaaaag
      781 cgttgctaaa taaggcatta ctacaaatgc ggccgagatg ctgagtccat tcaatcccat
      841 gtaagctcca cggtttttta cagacgatct ttttgcggca actgttgaca taaaagggag
      901 taccaaaatt tcggaaacac ttaaaaatgc cattgcaata aaacaagaaa ccagactagt
      961 gtcgaacagc aaccataaaa atgaaatgcc caaagcaaca caaccaatta ttaaaatagt
     1021 gtagatgtta aatctttttt caaataaagt aacaacaaac atttcggtaa gcaccacaaa
     1081 taatccgttg aaaccaagta acaatccaat ttcaaattgt gtaaaatgca acgattgact
     1141 gtaaaacaga ggcattatgc tgattaattg aaaaaagatg cacgaaaata aaaaacagta
     1201 aaaactaaaa atcaaaaacc atttgtcctt gtagggtgag tgttgcacag atgttgtcga
     1261 ttcttcgtgt tggttttcac taacattgcg ttcttttctt ttaaaaaagt aaacaaaaaa
     1321 tacaattgat gtagcaaaaa cagcaaaccc gttaccaaaa aatagccaag aatatgaaaa
     1381 tgttgcaagc atcccaccaa gagctggtcc cattgaaaaa ccaagattaa tagccagtcg
     1441 gtttaatgaa aacgaacggg ttaaatttcc ttttttagcg taagatgtaa tagccgcaga
     1501 attggctggt ctaaaacttt cataaatggt gcttaataaa aatatagaca cataaaattc
     1561 atgtggttgc ttaatataag ctaagaatac aaagagtggg gcagcaaaaa ataatgagaa
     1621 aaattgcact ttaaatgctc ccaccttatc agttagccaa ccaccaataa cagaacctaa
     1681 aagtgagcct attccaaaaa agctcattgc taatccagct tcatctgttt gaaatcctaa
     1741 aacagatgtt aaataaaccg taagaaaagg aatgaccatt ccacctgttc tattgattaa
     1801 catcaccaaa gataacatcc acgattctct ggatagtccc ttaaatgaat taaaatagag
     1861 ggcgaatgct tttttcaaaa tcttatgaat tttaaaaatt atagaaataa aaaaaagaga
     1921 gctttaaaaa agctctcttt tgctccccct gctggacttg aaccagcgac cctctgatta
     1981 acagtcagat gctctaaccg actgagctaa ggaggaattt tgttttctca attgcgagtg
     2041 caaatataag tgtatttttc ttttccgcaa gcaaaaaaat aagatttttt taaacttttt
     2101 tgtaagtcgt taaaaatcat catcattcaa tgaaaaatca gagaggttgg atgtgaaatc
     2161 atcgtcgtct tcgctatcat cttcgctata catatcgcca tcattaggat tggatacact
     2221 aggagctgaa cgaagaatgt ttgttttaaa tacgtttaat cgaatttctg tacgaatgct
     2281 tccgtcttca ttggtgtatg ctttggcaaa aggagtacct gctaattcaa caagtgttcc
     2341 tctttttaaa tagtcaacga cacgcatatt ggcgccttct cgtttccaaa tggtgcaatt
     2401 tacccatgta gtgtttgtac gttgctctcc ggttttttta tctttccaat ttttgttgtg
     2461 tgcaacagga aagttaatag caatcatacc actactcatt gattttacag ttgcatcttt
     2521 tccgatatta ccgattaatt ttgtttcaaa tactgtggcc atttttgttg ttttattgta
     2581 tgaaattaca aaaaaaataa tacaaacttc attttgctca tttttttcct tatttagaat
     2641 atgtatagtt agcattgaaa gttacttaaa atattatatt tttggaatcc aatcttatca
     2701 aatgaaagtt ttaacaatac atttttctca atctggacag ttaaccgaaa ttctaaccaa
     2761 tttcacacaa ccttttaatg gtgttgaaat ggaacatcaa ttcattgttc ctaaaaacaa
     2821 atttcctttt ccgtgggatg tgaaaacttt ttttgaaaca atgcccgaat cggtgttgga
     2881 aattccgatt gaactagaag caatcaatta taactattca aaatatgact tggtaattat
     2941 tggctatcaa ccttggtttt tatcaccatc tttacctata acttcgttat tgaaagacga
     3001 gcaatttaaa catatcataa aagacacgcc tattattact gtgattggct ctagaaatat
     3061 gtggcttaat tctcaagaaa gcgttaaaaa actaattcaa caagctggtg gaaaattggt
     3121 tggaaatgtt gccttggccg ataaaagcga taatttaatt ggtgtaatta ctattttgca
     3181 ctggatgttg aaaggagaaa aaacgcggaa atggggaatt tttccaaaac caggtgtttc
     3241 tgacgaagat atagaaaatg taaaattgtt tggagaattt gctgctcagg cgttgaaaaa
     3301 aaatgattgg aataattttc aaaatcaagt cattcaaaac gacggtgttg ttgttagaac
     3361 caatatttta tttattgaac aaaaagccaa acgtttattc atcatttggg caaatttaat
     3421 tttaaaacaa gcaaaaaaag gaaaacgcag cttttgggta aacgctttta aatattattt
     3481 aatttttgtg ttgtttgttg tttcgccaat tttgctaata ctttatcgag tgttaatttg
     3541 gcctttcaca tttaaaaaag tagaagcaag aaaaaaatat tttcagcaaa tagcaataaa
     3601 ataaaacgtg gcttttagcg tgtatggtgt aaatgtttaa agaaaaaaag ttattttagc
     3661 gacaaatttt tgaaatagaa caaactagga aatgatttta aaagacgttt atattacccg
     3721 aattgcatct tttctcccaa ataatcctgt ttcaaacgat gaaatggaag aatatttagg
     3781 cgttattaat caacaggctt ctaaatctag aagaattgtt ttgagaaata atggcattaa
     3841 aaatcggttt tatgcgttaa ccaaaggcgg taaaagcacc cattccaact cagaaatggc
     3901 tgctttatct attaaaaaat tatttcaaaa tccagcgcaa gaattagcaa cattggagtt
     3961 gattagttgt gcaacttcat cacccgaaca attaatgccc tctcatgcag tgatggtaca
     4021 cggacatttg ccagaaactc gcagcgtaga agtggtttcg ccttctggag tttgctgtgc
     4081 gggaatgcat gcgttgaaat atgcctatat gtctgtaaaa ctagggcaag ttaaacgagc
     4141 tgtttcttcg gcatcagaac ggttgtcgcc ttcattaaaa gccgataaat ttgaattgga
     4201 ggtaaaaaaa actaagcgag ttggaagaaa atccatacgt ttcttttgat aaagattttt
     4261 tacgttggat gctttcggat ggtgctggag cttttttagt tgaagataaa cccaatgaaa
     4321 attcaaactc tttgcgaata gattggattg aaggtttttc ctttgccaat gaaattgaac
     4381 cgtgtatgta catgggggca gaaaaacgcg aggatggttc tttgattagt tatttagatt
     4441 ttgaaccaca agaaagcatt g
//
LOCUS       DAOQVQ010000155         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21888_length_4445_cov_4.188610, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000155 DAOQVQ010000000
VERSION     DAOQVQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21888_length_4445_cov_4.188610"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PKN22_05905"
     CDS             complement(<1..781)
                     /locus_tag="PKN22_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNS42274.1"
                     /translation="MEYNYNAIEQKWRNRWAERKTYQVEQDANKQKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDVYARYKRLQGFNVLHPMGYDSFGLPAEQYAIQTGQHPELTTKE
                     NIARYRDQLDKIGFSYDWSREVRTSSPEYYKWTQWIFIELFNSWYDKTTDKAMPISEL
                     VATFEKNGNANINAVSDQEEVFSSEEWNNYSEEKKEAVLQNYRLTYLADSWVNWCPAL
                     GTVLANDEVVNGVSERGGHPVEQKLMRQWSMRIKAYAERLLT"
     gene            complement(889..1527)
                     /locus_tag="PKN22_05910"
     CDS             complement(889..1527)
                     /locus_tag="PKN22_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42275.1"
                     /translation="MKIIIACCLLLLSCTTIAQKPFVGKLVYSVEIADTALTKLFPPS
                     KMVIYTNDTLLRVETQTASLGDQILIQHLAKRKAYLLVASAKGNFAIQIPDVTPKASK
                     YTFKKGRGKKTIAGLKSAKLLVTHPDVTNEMEFFYNKKISAKYLPGFETYPGLLTEYY
                     TVTQDGVYRHTLVEISYNQPSKDLFGVPSDYQRISMDDFVDIMTGGKAKEGE"
     gene            complement(1636..2892)
                     /gene="metK"
                     /locus_tag="PKN22_05915"
     CDS             complement(1636..2892)
                     /gene="metK"
                     /locus_tag="PKN22_05915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNS42276.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDNFMAFDPTSKVACETL
                     VTTGQVVLAGEVKTTTYLDVQSIARETIKKIGYTRSEYMFDANSCGILSAIHEQSADI
                     NQGVERANPEDQGAGDQGMMFGYATKETDNYMPLALDIAHKILQELSHIRRNEPELIG
                     YLRPDAKSQVTIEYSDDNVPQRIDTIVVSTQHDVFGSEEEMLKKIKNDIISIVIPRVK
                     AKLRPEIQGLFNDNITYHINPTGIFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDPSKVDRSAAYATRHMAKNAVAAGLADEMLVQVSYAIGVAKPCGLFVDTYGTAKVN
                     MTDGEIANKLSEIFDMRPYFIEQRLKLRNPMYSETAAYGHMGRKNEIVKKTFVGPDGK
                     VIEKEVELFTWEKLDYVDKIKAAFGL"
     gene            3099..3770
                     /locus_tag="PKN22_05920"
     CDS             3099..3770
                     /locus_tag="PKN22_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42277.1"
                     /translation="MKNWMIACFAILGFMLTGCIEIIDDLKLNLDGSGTFKYSVNLSS
                     SKVKINSILALDTLDGQKVPSKEEIKEKISNFKFNLSQKKGISNVIIEEDFTNYIFKI
                     SCDFDDLSSLQTAIKRTISDLVSHQKNEFDEHEWITWDGNTLERSVPQIAMEQINKLK
                     KEDIELLKSGSYTCISRFDKEIKSCENPASVISKNKRAAMLRINTYDLSKNVDMLHNK
                     ITLID"
     gene            3945..>4445
                     /locus_tag="PKN22_05925"
     CDS             3945..>4445
                     /locus_tag="PKN22_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS42278.1"
                     /translation="MMTVKEIMAPVQDEINEFEIRFPESMQSNVKLLNRVTQFIVKRK
                     GKQMRPLFIFLTAKMVGKINDNTYDAACLVELLHTASLVHDDVVDDANERRGFFSANA
                     LWRNKIAVLVGDFMLSRVLLLSIERKNVELLEIVATSVREMSEGELLQLEKARSLDIT
                     EEVYFEV"
BASE COUNT         1379 a          822 c          791 g         1453 t
ORIGIN      
        1 cagtcaacag acgctctgca tacgctttga tacgcattga ccattggcgc attaattttt
       61 gttctactgg atgtccacca cgctccgaaa caccatttac cacctcatcg ttggcaagaa
      121 cggttcctaa agccggacac cagttaaccc aagaatctgc caaatacgtt aaacggtaat
      181 tttgcaatac tgcttccttc ttttcttcag aatagttgtt ccattcttcg gatgaaaaaa
      241 cctcttcttg atcgcttact gcattgatgt tagcatttcc gtttttttca aaggtagcca
      301 ccaattcact gatgggcatt gctttatcgg tagttttatc gtaccaactg ttgaacaatt
      361 caataaaaat ccactgtgtc cacttgtaat attcgggcga agaagtacgc acttctctac
      421 tccaatcgta tgaaaaccca attttatcca actgatcgcg gtaacgtgca atgttttctt
      481 tggtggtcaa ttcaggatgt tgccctgttt gaatggcata ttgttctgcc ggcaaaccaa
      541 aagaatcgta tcccattggg tgcaacacgt tgaaaccttg caaacgcttg taacgtgcat
      601 acacatcaga agctatgtaa ccgagtgggt gaccaacgtg caaaccagct ccagaaggat
      661 acgggaacat gtctaacaca taaaatttct gtttgttggc atcttgttca acttgatacg
      721 tttttctttc tgcccaacgg tttctccact tttgttcgat agcattataa ttgtactcca
      781 ttactcttta cttgaatttt gtacaaagat agttttttag tattgaagtt gaatcatcaa
      841 ttgtaaagtc gatggttttc tttttgttca tttcaatata aaataaacct actccccttc
      901 ttttgcttta ccacctgtca taatatccac aaaatcgtcc atggaaatgc gctggtagtc
      961 ggatggaacg ccaaacaagt cttttgaagg ctggttgtaa ctgatttcta ctaaggtatg
     1021 acggtacacg ccatcttgtg ttacagtata atattcggta agcaatccag gataggtttc
     1081 aaatccaggc aaatatttag cactgatttt tttattgtag aaaaattcca tttcgttagt
     1141 tacatcggga tgtgttacca ataatttagc tgattttaag ccggcaattg tttttttgcc
     1201 acgtcctttt ttaaaagtgt atttagatgc ttttggggta acatctggaa tttgaatggc
     1261 aaaatttccc ttagcagaag ctaccaataa ataggctttt cgctttgcta ggtgttgaat
     1321 caatatttga tcgcccaaag atgctgtttg tgtttctaca cgcaataagg tatcgttggt
     1381 gtatatcacc atttttgagg gcggaaataa tttggttaaa gcagtatctg caatttcaac
     1441 cgaatacacc aatttgccaa caaagggctt ttgggcaata gttgtacaag agagcaacaa
     1501 caaacaacag gcaataatta ttttcataca acgaaagtaa ggataatttt aaaacaattt
     1561 aaaacgtata atattgtgcc aaaaaaaaat ccctcccaaa attcgggaag gatttctcaa
     1621 aaaaatattt attgattaaa gaccaaaagc agctttaatt ttatctacat agtctaattt
     1681 ttcccatgta aacaattcta cttctttctc aattactttt ccgtctggtc caacaaatgt
     1741 tttcttaacg atttcgtttt tacgtcccat gtgaccataa gcagccgttt cagaatacat
     1801 tgggtttctc aatttcaaac gttgctcaat aaaataaggg cgcatatcaa agatttcgct
     1861 caatttattt gcaatttcac catctgtcat gtttactttt gcagttccgt aagtatccac
     1921 aaacaaacca caaggttttg ccacaccgat agcataagaa acttgcacca acatttcgtc
     1981 agccaaacct gcagcaactg catttttagc catgtgacgt gtagcataag ctgctgaacg
     2041 gtcaactttt gatggatctt ttccagagaa agcaccacca ccgtgtgcac ctttacctcc
     2101 gtatgtgtca acgatgattt ttctacctgt taaacctgta tctccgtgag gtccaccaat
     2161 tacgaaaata ccagttggat tgatgtgata tgtaatgtta tcgttgaaca acccttgaat
     2221 ttctggacgt aatttagctt tcacgcgtgg aattacgata gaaataatgt catttttaat
     2281 tttcttcaac atttcttctt ccgaaccaaa aacatcgtgt tgtgtagaaa caacgatggt
     2341 atcaatacgt tgaggaacat tgtcgtcaga atattcaatt gttacttgcg attttgcatc
     2401 aggacgtaag taaccaatca attctggttc gtttcttctg atgtgcgata attcttgcaa
     2461 aattttgtga gcaatatcca acgccaatgg catgtagtta tctgtttctt ttgtagcata
     2521 tccaaacatc attccttggt cgcccgcacc ttgatcctct ggattagcac gttctacacc
     2581 ttggttaata tctgctgatt gttcgtgtat agcagaaaga ataccacaag aatttgcatc
     2641 aaacatatat tctgagcgcg tataaccaat ttttttaatg gtttcacgag caatagattg
     2701 tacatctaaa taagtagttg ttttaacttc acctgccaat acaacttgac cagtggtaac
     2761 tagtgtttca catgccactt tcgacgtagg gtcaaaagcc atgaaattat caattaaagc
     2821 gtcagatatt tgatccgcta ctttgtcagg atgcccttca gaaacggatt cggatgtaaa
     2881 caaatacgac ataatattaa aattttaaat ataatagggt gcaaaattag aaaaaaaaca
     2941 agattgcaac ctaacatttt ttatttttta gcatttattc tcaataatct tttttgtggt
     3001 ttgatatttg tttgcaaaat cgcatacatt tgtgccgtga attttaaatg aacattactt
     3061 tagctaaaat tcatgctgct gattttaaat gaattgatat gaaaaattgg atgattgcat
     3121 gctttgcaat tcttgggttt atgttaacag gttgcattga aattattgac gacctaaaat
     3181 taaacctcga tggttcggga acttttaaat attcggtaaa cctttcatcg agtaaggtga
     3241 aaatcaattc tattttggca ctcgacactt tagatgggca aaaagtgcct tccaaagaag
     3301 aaattaaaga aaaaatcagc aattttaagt tcaacctcag ccaaaagaaa ggtatttcta
     3361 acgtaattat tgaagaagat tttaccaatt atatttttaa aatcagttgc gattttgacg
     3421 atttaagttc actgcaaacg gccatcaaac gaaccatcag cgatttggtt tcgcaccaaa
     3481 aaaatgaatt cgacgaacat gaatggatta catgggatgg caatactttg gaacgttctg
     3541 tgccgcaaat tgctatggaa caaatcaata aattgaaaaa agaagatata gaattattaa
     3601 aatcaggttc ctacacgtgc atttctcgtt ttgataaaga aattaaaagt tgtgaaaatc
     3661 cggcttctgt aatatctaaa aataaaagag cagcgatgtt gagaatcaat acttatgact
     3721 tatcgaagaa tgtcgatatg ttgcataata aaattacctt gatagattga ttcagctagc
     3781 ttttttacgt ttgatgatgt tgaaaatcaa tatttttgac ttatcgaaat atgtcgatat
     3841 gatgaataat aaaattacct tgagagatta atttcaacca gctcttttac gttttatttg
     3901 aaacaatcat ttttacgtgg cattttgtta tctttgcacc tgcgatgatg acagtcaaag
     3961 aaataatggc accggtgcaa gatgaaatca acgaatttga aattcgattt ccagaaagca
     4021 tgcagtccaa tgttaagtta ctcaaccgag tgacacaatt catcgtgaag cgaaaaggca
     4081 aacaaatgcg tccgcttttt attttcttga cagctaaaat ggtcggaaaa atcaacgaca
     4141 acacctatga tgctgcttgt ttagtggaat tgttacacac agcatctttg gtgcacgacg
     4201 atgtggtgga tgatgccaat gaacgtagag gttttttctc ggccaatgcc ctatggagaa
     4261 ataaaattgc agtactggtt ggagatttca tgctctctcg tgttttattg ctgtctatcg
     4321 aacgtaaaaa tgttgaatta ctagagattg ttgctacatc tgtacgcgaa atgagtgagg
     4381 gtgaattgtt gcaattggaa aaagcaagaa gtttagatat taccgaagag gtttattttg
     4441 aagtc
//
LOCUS       DAOQVQ010000156         4444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21899_length_4444_cov_3.196400, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000156 DAOQVQ010000000
VERSION     DAOQVQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21899_length_4444_cov_3.196400"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKN22_05930"
     CDS             <1..779
                     /locus_tag="PKN22_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42279.1"
                     /translation="NIADTVNAALSPVGPLWRQLRTDYPALELYSSDESHPSTAGSYA
                     AACSFYAVMFKRPVAAINYNFSLNATDAATIRTVAQQVVYDNLSYWQRFEPQTTSDFT
                     TQPQVGTTNMQFSNLAENASSVKWNFGDNTESTTENPTHTYAQEGDYTVCLTAYVGQN
                     CDSVTICKTVTIAELGLNATVSRSTIELYPNPTTDVVTIKGELTNGTYEIVDVLGNKV
                     QQGVLKTDKTTLQVQALKTGVYFIFIHSAEGKQQLRFVKK"
     gene            835..1437
                     /locus_tag="PKN22_05935"
     CDS             835..1437
                     /locus_tag="PKN22_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS42280.1"
                     /translation="MHEQNYANELDANFWNSRWQTGHIGWDIGCASPPLTEYIQTLND
                     KNLAILIPGCGNAYEAEYLVANGFTNITLIDIAEEAVRRLKEKFRFDSQVTIICGDFF
                     EHQGSYDLIIEQTFFCAQVLQRRTEYAQKIHSLLKPNGKLVGVLFGVDFGPQGPPFGG
                     DIHEYQQLFKPYFELKKLEPCFNSIKPREGAELFIEFIKK"
     gene            1434..1709
                     /locus_tag="PKN22_05940"
     CDS             1434..1709
                     /locus_tag="PKN22_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNS42281.1"
                     /translation="MSSSKLVLIDFYADWCSTCQTMEPTIDAIQEKFGVQLEILKINT
                     EENPQMAALYHIRSVPTYILFKNGEIVWKVAGLLTKKEFIQTIEKQL"
     gene            complement(1719..3326)
                     /locus_tag="PKN22_05945"
     CDS             complement(1719..3326)
                     /locus_tag="PKN22_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42282.1"
                     /translation="MKILIFLSLFFTASTVFSQDFSILTQKSIGGTGDDSGRLLPDPQ
                     NNGEYFLISSSKSGISGDKTEPSNGGVDVWIVKLDNNFNVLWDKSYGGSGDDVFWSAF
                     INNGFIYIATQTSSNQSGDKTMDLFSTEPYPSNIWILCLDLSGNLIWQQQFGGTNVET
                     RPYLIPYTQTTFLMSCSSRSPVSGNKTTALKGLDDTWLVEVNYSDGSIAQQKSVGTDA
                     NDNVKSIKRVSNGNILLSITTGGDINGDKTVSSFGLDDIWLIEMDNSLNIVKQSGFGG
                     NSVEDRGYIAEIEDAYYLTISTYSDASGNKSENSFGDTDVWLVKMDKDFTIIWDKTIG
                     GNYTEYGGTVFKNANGKLVLFATSQSIPSGNKTSPRYGSSYDSWIVLLNEDGDLLEQY
                     TLGGNGSDGCEIIEYPNDPTKLLFYGASDSPMGSGVKTSPVIGGSDIWLGVIDASNVL
                     TTSNLTVGEFSVYPNPTKNSLFINSSSEVEYQAWISDLNGKQVSSIKKNTFIGEAVLD
                     VKSINSGVYFLNISTKFGNQIKKIIIE"
     gene            complement(3423..>4444)
                     /locus_tag="PKN22_05950"
     CDS             complement(3423..>4444)
                     /locus_tag="PKN22_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42283.1"
                     /translation="PGGHPYKQQTFTGIDEQYLYRRMLINSSMFSGPTPYRDKFNNPV
                     SDGFLIEKKLDEFTHLNGSMTVETAVPNTDICAVNDINYWKNFFNQYVDLNPVNIPSS
                     VEPFAYTVPVGVECLETYDHTGVTSGDVLDNDWNAWVKELYESVDYTEEAIGSSSSSG
                     HGKLFNELISQFNQLLNTSFSGDSVSFIVLESIDKRNFSEAIIKKDVNGYYLLYSTGL
                     FQKGLYNISFRSAKGNVFPTVFKISSDLVVTQLKEDVDVIAYPNPIVNDQLNLKIDSK
                     QALLARLLLSDNEGNLIINEEIKLKNGISNYTYDLSSIKNSNYLVLNVVYPDGSVDHL
                     TLIKN"
BASE COUNT         1527 a          845 c          660 g         1412 t
ORIGIN      
        1 gcaacattgc ggatactgta aacgcagctt tgagtccggt tggtcctttg tggcgacaat
       61 tgcgaacaga ttatcctgct cttgaacttt attcgagcga tgaaagtcac ccatctacgg
      121 ctggatccta cgctgcggca tgtagttttt atgctgtaat gtttaaacgt ccagtggctg
      181 ctatcaatta taatttttcg ttgaacgcta ccgatgcggc aaccatccga acagtggcgc
      241 aacaagttgt ttatgacaat ttaagttatt ggcaacgttt tgaacctcaa acaacaagcg
      301 attttacaac acaaccacaa gtgggcacaa ccaacatgca atttagcaac cttgcagaaa
      361 atgcttcttc ggttaaatgg aattttggtg acaacacaga aagcacaaca gaaaatccta
      421 cgcacaccta cgcgcaagaa ggagattaca ccgtttgctt aaccgcttat gtgggtcaaa
      481 actgcgattc tgtaacaatt tgcaaaacag taactattgc cgaattagga ctaaacgcaa
      541 cagtaagtcg ttctaccatt gaactctatc caaacccaac aaccgatgtt gtaaccatca
      601 aaggagaact aaccaatgga acctatgaaa tagtagatgt attgggcaat aaagtgcaac
      661 aaggtgtttt aaaaaccgac aaaacaaccc ttcaagtgca agcattaaaa actggcgtat
      721 attttatttt cattcattct gcagaaggta aacaacagtt gcgttttgtg aagaagtaga
      781 atattactcc catcgcataa ttgttttgta tatttgtaaa acaaaaaaaa tacaatgcac
      841 gaacaaaatt atgcgaacga attggatgct aatttttgga attcacgttg gcaaacagga
      901 catataggct gggacattgg ctgtgcttct ccaccgctta cagaatacat acaaaccctt
      961 aatgataaaa atttagccat tctcattcct ggatgtggaa atgcgtatga agccgaatat
     1021 ttggttgcaa acggttttac caacatcact ttgatagata ttgccgagga agccgttaga
     1081 agattaaaag aaaaatttcg attcgattct caagtaacta tcatttgtgg cgatttcttt
     1141 gaacaccaag gcagttatga cctcatcata gaacagacat tcttttgcgc acaagtattg
     1201 caacgaagaa ctgaatatgc acaaaaaata cattcattac ttaaaccaaa cggcaaatta
     1261 gttggcgttt tatttggtgt tgattttggt ccgcaaggtc cgccatttgg aggcgacatc
     1321 cacgaatatc agcagctttt taaaccctat tttgaattga agaaattgga accgtgcttc
     1381 aatagtatta agccaagaga aggggctgaa ttattcattg aatttatcaa aaaatgagtt
     1441 cctcaaaatt ggttttaatt gatttttatg cggattggtg cagcacttgt caaacaatgg
     1501 aaccaaccat tgatgctatt caagaaaaat ttggggtaca actggaaatt ttaaaaataa
     1561 acaccgaaga aaacccacaa atggcagcac tttaccacat tcgaagtgtg cctacttaca
     1621 tccttttcaa aaatggtgaa attgtatgga aagttgctgg tttattaacc aaaaaagagt
     1681 ttattcaaac aattgaaaag cagttataaa tctacaattt attcaataat tattttctta
     1741 atttgattac caaacttagt agatatattt aaaaaataaa ctccactatt tattgattta
     1801 acatctaaca cggcttcacc gataaaggtg tttttcttaa ttgaagatac ctgtttacca
     1861 tttaaatccg aaatccacgc ttgatactct acctcactag acgaattaat gaatagacta
     1921 ttttttgttg gattaggata tacactaaat tcacctactg tcaaattaga agtggtcaat
     1981 acatttgaag catcaataac ccctaaccat atgtcagaac caccaatcac tggtgatgtt
     2041 ttaactccgg aacccattgg gctatccgac gcaccataaa acaacaattt tgtagggtca
     2101 ttaggatact ctattatttc acaaccatct gatccattac ctcctagtgt atattgttct
     2161 agtaaatctc catcctcatt tagaagaact atccacgaat cataagatga accatacctt
     2221 ggtgaagtct tgttgccact tggtatggac tgtgaggttg caaaaagaac caacttgcca
     2281 tttgcatttt taaacaccgt tcctccatac tcagtgtagt taccaccaat cgtcttgtcc
     2341 caaataatag taaagtcttt gtccattttc actaaccata catctgtatc accaaaagaa
     2401 ttttctgatt tattaccaga agcatcggaa taagtcgaaa tagttaaata ataagcatct
     2461 tcaatttctg ctatatatcc tctatcttca acagaatttc cgccaaaacc tgattgtttt
     2521 acaatattta aagaattatc catttcaatt aaccatatat catccaaacc aaaactgctt
     2581 accgttttat ctccattaat atctcctcca gtagtaattg ataataaaat attaccatta
     2641 gaaactcttt taatgctctt aacattatca ttggcatctg ttccgacact tttttgttga
     2701 gctattgaac catcagaata gtttacctca accaaccaag tatcgtctaa cccctttaaa
     2761 gcggttgttt tattgccaga cactggagat ctagacgaac aagacattaa aaacgtggtt
     2821 tgtgtatatg gtattaaata aggacgcgtt tcgacattag ttccaccaaa ttgctgttgc
     2881 caaatcaaat tgccagataa atctaaacac aatatccaaa tatttgaagg ataaggttct
     2941 gttgaaaaca aatccattgt cttatcccct gattgatttg aggaagtttg agtagcaata
     3001 taaataaaac cattgtttat aaaagctgac caaaacacat catccccact accaccataa
     3061 gatttatccc acaatacatt aaaattatta tctaatttta caatccatac atcaacacct
     3121 ccgtttgaag gctctgtttt gtcacccgaa ataccggatt ttgaggatga aataagaaaa
     3181 tattcaccgt tgttttgagg gtctggtagt aatctaccac tatcatcgcc agtgccccca
     3241 atactttttt gagttagtat tgaaaaatct tgagaaaaaa ctgtactagc agtaaaaaac
     3301 aaacttaaga atattagaat tttcatagct tttagattaa aaagtgtagt cattaacgtt
     3361 catgactaca ctcagttatt gtataaaaat ataaataaat taatttatac aactatttcg
     3421 tattaatttt ttattaacgt taaatgatct acactcccat ctggataaac tacgtttaaa
     3481 accaaataat tagaattttt tatagaactc aaatcataag tataatttga aataccattt
     3541 ttcaacttaa tttcttcatt tattattaaa tttccttcat tatcacttaa taacaatcta
     3601 gctaacaatg cttgttttga gtctatttta aggtttaatt gatcatttac aattggatta
     3661 ggataagcta ttacatctac atcctctttt aattgagtta caactaaatc agaagaaatt
     3721 ttaaatactg ttggaaaaac gtttcctttt gctgatctaa atgaaatatt atataaacct
     3781 ttttgaaata aaccagtaga atataaaaga taataaccat ttacatcttt tttaattatt
     3841 gcttcagaaa aatttctttt gtcgattgat tctaaaacaa taaaagaaac actatctccc
     3901 gagaaacttg tatttaataa ttggttgaac tgactaatta attcgttaaa taatttacca
     3961 tgaccagaag aactggatga tccaatagct tcttctgtat aatcgactga ttcatacaac
     4021 tccttaaccc atgcattcca gtcattatct aatacatctc cagatgttac accagtatgg
     4081 tcgtacgttt ctaaacactc aactccaact ggtacagtat aagcaaaagg ctcaacacta
     4141 ctaggaatat taactggatt caaatcaaca tattgattga aaaaattttt ccaataatta
     4201 atatcattta cagcacaaat atccgtattt ggaactgctg tttcaactgt catacttcca
     4261 tttagatgtg taaattcatc taatttcttt tctatcaaaa atccatcaga aacaggattg
     4321 ttaaacttat ctctatatgg ggttggaccg ctgaacattg aactattaat taacattctt
     4381 ctatacaaat attgctcatc tatcccagta aatgtctgct gtttatatgg atggccacct
     4441 ggta
//
LOCUS       DAOQVQ010000157         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21920_length_4440_cov_3.277081, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000157 DAOQVQ010000000
VERSION     DAOQVQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21920_length_4440_cov_3.277081"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKN22_05955"
     CDS             complement(<1..590)
                     /locus_tag="PKN22_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNS42284.1"
                     /translation="MSSAVNKKSGLGKGLGALLGNEASAPAQKGEAGSIAMIAIEEIE
                     ANPFNPRTHFEREALEELSQSIAIHGIIQPLTVRKVGQDKYQLISGERRFRASQLAGL
                     KEVPAYVRVANDQTMLEMALVENIQREDLNAIEVALSYQRLIDECSLTQDQLSQKVAK
                     SRSSITNFLRLLKLPAEIQAGVRDNVISMGHARSLVS"
     gene            complement(583..1389)
                     /locus_tag="PKN22_05960"
     CDS             complement(583..1389)
                     /locus_tag="PKN22_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS42285.1"
                     /translation="MGKIIAIANQKGGVGKTTTSINLAACLGVLEFKTLLVDADPQAN
                     ATSGVGLDPKNARNIYDCLVNDVNPKELIITTDSPNLDILPSHIDLVGAELEMINIPN
                     REHKLKMALDKIKDEYDFIIIDCSPSLGLITVNALTASDSVLIPVQCEYFALEGLGKL
                     LNTIKIVQGRLNTNLQIEGILLTMYDTRLRLANQVVDEVKAHFQDLVFDTLIHRNTKL
                     GEAPSFGQTIVMHDISSKGAINYLNFARELLQKNDLTKISNNDKNYSINE"
     gene            complement(1506..2852)
                     /gene="ffh"
                     /locus_tag="PKN22_05965"
     CDS             complement(1506..2852)
                     /gene="ffh"
                     /locus_tag="PKN22_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNS42286.1"
                     /translation="MFENLTDKFERAFKVLKGQGQITEINVAETLKEVRRALLDADVN
                     FKTAKEFTNTVKEKALGRDVLTAVSPGQLMVKICHEELVELMGGNEVDINIKGNPGII
                     LMSGLQGSGKTTFTGKLGNYLKTKKHKNVLLVACDVYRPAAIDQLHVLGEQLGIEVFS
                     NKEEKNPVLIAQAAVQHAKSKGFHVVIVDTAGRLAIDEQMMNEIAAVKNAINPTETLF
                     VVDSMTGQDAVNTAKAFNDKINFDGVVLTKLDGDTRGGAALSIKSVVDKPIKFVGTGE
                     KMDALDVFYPSRMADRILGMGDVVSLVERAQEQFNEEEARKLQKRIHKNQFDFNDFLG
                     QLQQVKKMGNVKDLMGMIPGMGKALKDVEIEDDAFKHIEAIIYSMTPKERENPDIINQ
                     SRKNRIAKGSGTTIQDVNKLLKQFGDTKKMMKMMSNPKNMMNMMKQMQGMKGMPGM"
     gene            complement(2977..>4440)
                     /gene="htpG"
                     /locus_tag="PKN22_05970"
     CDS             complement(2977..>4440)
                     /gene="htpG"
                     /locus_tag="PKN22_05970"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNS42287.1"
                     /translation="AQAVQWESNGSPEYTIIDIEKETRGTDIVLFIAEDSVEFLEKAR
                     VQEILSKYCKFLPIPVVFGTKTEYIDSPEGEKDEDGKVKRVAVDVENQVNNPTPAWTK
                     APIDLKAEDYDAFYRELYPMTFESPLFHIHLNVDYPFNLTGILYFPKIKENVEVQRNK
                     INLYSNQVFITDSVENIVPEFLTLLHGVIDSPDIPLNVSRSYLQSDGNVKKISAHITK
                     KVADKLAEMFKNDRADFESKWEDLQVFVQYGTLSEEKFAEKAKAFSLVKSTDGKFYTL
                     DEYKDKIAALQTNKEGKTVILYTNNADLHFGFIKKANERGYDVLNFDSPLTPHWISKM
                     EQTLENVSFARVDADTLDKLIQKDEVIPSKLSKEDEEKLKPIFENSLAEKDKFTVTFE
                     SMSASESPIIITQPEFIRRMMEQQRMGGAGFFGAFPERYNVVVNSNHEKITEVMNASD
                     DEKGKKVKQLTDLALLSQGMLTGEALNAFIERSIELV"
BASE COUNT         1261 a          950 c          715 g         1514 t
ORIGIN      
        1 gatactaacg aacgagcatg ccccattgaa ataacattat cacgcactcc agcttgaatc
       61 tcagctggca atttcaataa acgcaagaaa ttggtaatgc ttgaacgtga ttttgccact
      121 ttttgactca actgatcttg ggtaagtgaa cattcatcta tcaaacgttg ataggatagt
      181 gcaacttcaa tcgcattcaa atcttcgcgt tgaatgtttt ccaccaaagc catttccaac
      241 attgtttgat cattggcaac gcgcacatac gcaggcactt ctttcaatcc agccaattgc
      301 gaagcacgaa aacgacgttc tcccgaaatt aattggtatt tatcttgtcc aactttacgc
      361 accgttaacg gttgaataat tccgtggatg gcaattgatt gactcaattc ttccaatgct
      421 tcacgttcaa aatgagtacg aggattaaac ggattggctt caatttcttc aatagcaatc
      481 atagcaattg aacctgcttc tcctttttga gcgggtgcac ttgcttcgtt tcctaaaagt
      541 gctcccaatc ctttccctaa tcctgatttt ttatttacag cactactcat tgattgaata
      601 atttttatcg ttatttgaaa tttttgtcaa gtcgtttttt tgcaataatt cgcgtgcaaa
      661 atttaggtaa tttatggctc ctttgcttga aatgtcgtgc ataactattg tttgaccaaa
      721 gcttggagcc tcgcccaatt tagtattgcg gtgaatcaag gtatcaaaca ccaaatcttg
      781 gaaatgtgct tttacctcat ccaccacttg atttgccaaa cgaagacgcg tatcgtacat
      841 ggtcaacaaa attccttcaa tttgaagatt ggtattgagg cgaccttgaa caattttaat
      901 cgtattgagc aatttaccca atccttccaa ggcaaaatat tcgcattgca ccggaattaa
      961 aacagaatcc gaagcagtta aagcatttac agtaatcaat cccaacgatg gcgagcagtc
     1021 gatgatgata aaatcgtatt catctttgat tttatccaag gccattttca atttatgctc
     1081 tctgtttgga atgttaatca tttccaattc agcccccacc aaatcaatgt gcgatggaag
     1141 aatatctaaa ttcggactat cggttgtgat gattaactct tttgggttca catcgttgac
     1201 caagcaatcg taaatattgc gtgcgttttt aggatccaac cctactccac ttgttgcatt
     1261 ggcttgtggg tcggcatcaa ccaacaatgt tttaaattcc aacacaccca agcaagctgc
     1321 aaggtttatt gacgtagtag ttttcccaac tccacctttc tgatttgcta ttgcaatgat
     1381 tttgcccatt ttttatccgt ttgaagagct aaagataata cgaaatagga tacaaattaa
     1441 tgtaaaccaa taatagtttt caacgttttt tttacagtaa tactaacaat ttaattatga
     1501 aaaaactaca tgcccggcat acctttcatc ccttgcattt gtttcatcat gttcatcatg
     1561 ttcttaggat tactcatcat tttcatcatt tttttggtat caccaaattg tttgagcagc
     1621 ttgtttacat cttgaatagt tgtaccgcta ccttttgcaa ttctattttt gcgagattga
     1681 ttgatgatat ctggattttc gcgctctttg ggtgtcatcg aataaatgat agcctcaatg
     1741 tgtttgaaag catcgtcttc aatttcaaca tctttcaagg cttttcccat tcctggaatc
     1801 attcccatca aatctttcac attccccatt tttttcactt gttgcaattg ccccaagaaa
     1861 tcattgaaat caaactgatt tttgtgaatg cgtttttgaa gtttgcgcgc ttcttcttcg
     1921 ttgaattgtt cttgtgcacg ttccaccaaa gaaaccacgt cacccattcc caaaatacga
     1981 tcggccatac gcgaaggata gaacacatcc aatgcatcca ttttttcgcc cgtaccaaca
     2041 aatttgatgg gtttatcaac caccgatttg attgacaatg cagcaccacc tcttgtgtca
     2101 ccgtctaatt ttgtcaatac aacaccgtca aaattaattt tatcgttaaa tgcttttgct
     2161 gtattcactg catcttgccc cgtcattgaa tccacaacaa acaacgtttc tgttggattg
     2221 atggcatttt taacagccgc aatctcgttc atcatttgtt cgtcaatagc caaacgacca
     2281 gctgtatcga caatcactac atgaaaacct ttgcttttag catgttgaac agccgcttga
     2341 gcaatcaata caggattttt ttcttccttg tttgaaaaaa cttcaattcc caattgttca
     2401 cccaatacgt gcaattggtc aatcgctgcc ggacggtaca cgtcacaagc aaccaacaat
     2461 acatttttat gttttttagt tttgaggtag ttacccaatt ttccggtaaa tgttgtttta
     2521 cccgaacctt gaagtcccga catcaaaata attcccggat tgcccttgat gttgatatcc
     2581 acttcgtttc cacccatcaa ttctaccaat tcttcgtggc aaattttcac cattaactga
     2641 ccaggcgaaa cagcggtcaa cacatcacgt ccaagtgctt tttccttaac tgtgtttgta
     2701 aattcttttg ccgttttaaa attaacatcg gcatctaaaa gtgcacgacg aacttctttc
     2761 aatgtttctg ctacgttgat ttctgttatt tgtccttgtc cttttaatac tttaaaggcg
     2821 cgttcaaact tgtcggtaag attttcaaac ataattattt tggattttgg atagcaaaga
     2881 tattattttt ctttgagata tggcgtaaaa tgaagttggt tggcacaaaa aatcatacca
     2941 accaacataa aaattctaat gaatcaaaaa acgttgttaa accaattcga tgctgcgttc
     3001 aataaatgca ttcaatgctt cgcctgtcaa cattccttga gaaagcaagg caagatcggt
     3061 taactgtttt acttttttcc ctttttcatc gtcagaggca ttcattactt ctgtaatttt
     3121 ctcatgattg ctgttcacca ccacattata gcgttctggg aaagcaccaa agaaaccagc
     3181 accacccatg cgttgttgct ccatcatacg gcgaataaat tcgggttgtg taatgataat
     3241 tggcgattct gaagcactca tgctttcaaa tgtcaccgta aatttatctt tctctgccaa
     3301 tgagttttca aaaattggtt tgagtttttc ttcgtcttct ttggataatt tggatggaat
     3361 tacctcgtct ttttgaatca atttatccaa agtatcggca tccacacgtg caaaagaaac
     3421 attttctaag gtttgctcca ttttactaat ccagtgtgga gtaagcggac tatcaaaatt
     3481 caacacatca tagcctcttt cattggcttt tttgataaat ccaaagtgca agtcagcatt
     3541 attggtgtat aaaatcaccg ttttaccttc tttattggtt tgaagtgctg caattttatc
     3601 tttgtattca tcaagtgtat aaaatttacc atcggttgat tttaccaagc taaatgcttt
     3661 tgctttttca gcaaattttt cttcagaaag agttccgtat tgtacaaaca cttgcaaatc
     3721 ttcccatttt gattcaaaat cagcacgatc gtttttaaac atttctgcca atttatcagc
     3781 cacttttttg gtaatgtgcg ccgaaatttt cttcacattg ccatcgcttt gcaaataaga
     3841 acgagaaaca tttaaaggaa tatcaggaga atcgataact ccgtgcaaca acgttaaaaa
     3901 ctccggaaca atattttcaa cactgtctgt aataaacact tggttagaat acaagttgat
     3961 tttattgcgt tgcacctcca cattttcttt gatttttggg aaatacaaaa tacctgttaa
     4021 gttgaacgga taatcaacat tcaagtgaat atgaaacaac ggactttcaa aagtcattgg
     4081 atacaactca cggtaaaatg catcgtaatc ttctgctttt aaatcaattg gtgctttggt
     4141 ccaagcaggc gttgggttat tcacctgatt ttctacatca acagccacac gtttcacttt
     4201 tccatcctcg tctttttctc cttctggcga atcgatgtat tctgtttttg taccaaacac
     4261 aacaggaatg ggcaagaatt tacagtattt gctcaaaatt tcttgaacac gtgctttttc
     4321 caaaaattct actgaatctt ctgcaataaa caacacgata tcggttccgc gcgtttcttt
     4381 ttcgatatca atgatggtgt attctggcga accattactt tcccattgta ctgcttgcgc
//
LOCUS       DAOQVQ010000158         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_21961_length_4434_cov_3.513816, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000158 DAOQVQ010000000
VERSION     DAOQVQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21961_length_4434_cov_3.513816"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKN22_05975"
     CDS             <1..327
                     /locus_tag="PKN22_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNS42288.1"
                     /translation="DGFELLAPVKSYDKGQLGLYDIQGNVWEWCMDPYHQNAYQYVRN
                     WKVNSTQPLVPNYFDEFSPTEPTYVIRGGSFLCSNNYCEGFKASKRMRSSSQTTFQHI
                     GFRCVK"
     gene            complement(371..622)
                     /gene="purS"
                     /locus_tag="PKN22_05980"
     CDS             complement(371..622)
                     /gene="purS"
                     /locus_tag="PKN22_05980"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNS42289.1"
                     /translation="MRFKAEIDVMPLDALLDPQGKAVSNSMKSIGLPEIEGVRIGRHI
                     RLFVEAASKSEAESKVEEACKKLLANQIMESYTFVVVEA"
     gene            complement(619..1419)
                     /locus_tag="PKN22_05985"
     CDS             complement(619..1419)
                     /locus_tag="PKN22_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNS42290.1"
                     /translation="MFNIPNLFTAGNLLSGVCSIILAFSGQLELACFLIYFAAILDFF
                     DGFLARLLNQKSELGKQLDSLADMVTFGVAPGILVCLVLVIQTTDLRYIPGLTFNEFV
                     GMKIGQFLHLLSFGQWEMINWICLAGLIIPFFSMFRLAKFNIDTRQSESFIGLPTPAN
                     TIFFTAFPLLLASNYEQSPNAVLDFLTTPWVLVVITVVMSLLLVAELPLFSLKFKHFK
                     WKGNEVKFVFLLTCLVLIPLLLAWSILIIITLYLILSIIENKRQKLTT"
     gene            1556..2686
                     /locus_tag="PKN22_05990"
     CDS             1556..2686
                     /locus_tag="PKN22_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNS42291.1"
                     /translation="MTKYIFALAIALFTSSVYAQTVAPKYSNEFLQIGIGADALGFGN
                     AVVANTGSAMSGYWNPAGLTQSDKWMDIGLMHSEYFAGIAKYDYLGLSHKIDEKSAFG
                     FTAIRFGVDDIPNTTQLIDNQGNIDYDRITTFTAADYGFLFSYARKLPIKGLSFGGNF
                     KVIYRQVGKFAKSWGFGLDAGLQYQLNDHWKFGAVVRDVTSTFNAWIFTLDDQTKEVF
                     TQTGNEIPKNGLELTLPRIILGAYTKYPIGKKGIYFASELDLDFTTDGKRNTLIRSKV
                     FSIDPKFGIEFGFKHFVSIRAGITNMQFTKDFNDKKKFNIQPNIGIGLGFKSVRLDYA
                     FTDIGDASVALYSHVISLRVGLNKPKNAVKKPKESEKPKTSE"
     gene            2699..>4434
                     /locus_tag="PKN22_05995"
     CDS             2699..>4434
                     /locus_tag="PKN22_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNS42292.1"
                     /translation="MKLTRHILLIVCYLFLLGNWANAQTYGNEWIDYNQTYYSFKIYQ
                     TGLKRINYDDLYTAGVPLGTFTHENIQLFGREKQVPIWVETNGDGTFDSGDYILFYAE
                     KNDGWLDTTLYLDPTKSANPTISLFNDTINYFFTWNNQTNNLRFTVEDGSDYTNYTPI
                     DFVWNKFEQGYANAYLEGETLQSLISSSFYVSGEGYGLGSVNGVNGYNLPLVASTPFP
                     YTNTGAPQALFKGKVSTTASAAIQNAGDNNHHTQFSINGQVVFDQMGYGHFQFNANVP
                     FSTSLLGTPTTPLQWSIVGDLPVATMYESLTYWSIEYPRIPNFQGLTKFKVNVPNSNF
                     SKTRVDCSNFSITNPVVFSFGSTPSKITPITDGANQVLVLPINTQDSVSTIIAESLNS
                     AQTITAFNLVNGTGKFTDFASLNHEKAYIIVYPPKLTAGANDYKLYRQSIAGGAHNVI
                     MANVEELYLQYGGGIGKHINAIRRFAHQTYNLSTEKPIALFLLGKALYPGYSRFNPTN
                     YSNNLVPTFGTPPSDILITSNLLGTTKWAPLIPTGRLSVDSDAGVANYLEKITDYETH
                     QNQSDVYDSPNK"
BASE COUNT         1511 a          835 c          735 g         1353 t
ORIGIN      
        1 gatggatttg aactgcttgc ccctgttaaa agttatgata aaggacaatt gggactttac
       61 gacatacaag gtaatgtatg ggaatggtgc atggatcctt atcaccaaaa tgcttatcaa
      121 tatgtcagaa attggaaagt aaatagcaca caacctctag ttcccaacta ttttgatgaa
      181 ttttcaccaa ctgaaccaac ctacgtgatt cgtggcggtt catttttatg ctctaacaat
      241 tattgcgaag gatttaaagc ttcaaaacgt atgcgatctt cttcacaaac aacctttcaa
      301 cacataggtt ttaggtgtgt aaaataaaaa agcccgccag aatagcgagc ttttcaaata
      361 tgcttttaat ttatgcttct actacaacaa atgtgtagct ttccataatt tgatttgcta
      421 acaatttttt gcaagcctct tctactttgc tttcagcttc actttttgat gccgcttcaa
      481 caaacaaacg aatgtggcga ccaattcgaa ctccttcaat ttcaggcaat ccaatgcttt
      541 tcatactgtt tgaaacagct tttccttgtg ggtctaacaa tgcatctaat ggcattacat
      601 ctatttctgc tttaaatctc atgttgtgag tttttgtcgt ttattttcta ttatcgataa
      661 aatcaagtac aaagttataa taattaaaat agaccacgct aataaaagtg gaattaatac
      721 taaacatgtt agtaaaaaaa caaattttac ctcattaccc ttccatttaa aatgtttgaa
      781 ttttaaagaa aagagtggta attctgccac taacaaaagc gacataacaa cagtaataac
      841 aaccaaaacc caaggtgtag ttaaaaaatc taaaactgca ttcggtgatt gttcataatt
      901 tgaagccaat aacaacggaa atgctgtaaa aaatatcgtg ttggctggtg ttggcaaacc
      961 tataaaactt tcagattgac gggtatcaat attgaatttt gctaatcgaa acatggaaaa
     1021 aaatggaatt attaatccag ctaagcaaat ccaattaatc atttcccact gaccaaaact
     1081 gagtaaatgc aaaaactgcc caattttcat tcctacaaac tcattaaacg taagccctgg
     1141 aatgtaacgc aaatcagttg tttgaattac caacaccaaa caaactaaaa tacctggcgc
     1201 aacaccaaaa gtaaccatat cggccaacga atccaattgc ttgcctaatt cacttttttg
     1261 attcaataag cgtgctaaaa atccatcaaa aaaatcaaga attgcagcaa aataaataag
     1321 aaaacaagcc aattccaact gtccagaaaa ggctagaatt attgagcaaa caccactcaa
     1381 taaattccct gcggtaaata aattgggtat attaaacatt ctgggcacta aaatacacat
     1441 ttagatgtcc taaattaagt attttcgttt atttttgttt tttaatagag gatattttta
     1501 accgctatac aatttttaag acacaaatca gttattgttt gtgaaattaa cacttatgac
     1561 aaaatacatt tttgcgcttg caattgctct ttttacctct tctgtttatg cacaaacagt
     1621 tgcacccaaa tattccaatg aatttttaca aattggtatt ggcgccgatg cacttggttt
     1681 tggaaatgcc gttgttgcca atacaggaag cgctatgtcg ggatattgga atcctgccgg
     1741 acttacgcaa tctgataaat ggatggacat tggcttaatg cacagtgaat attttgctgg
     1801 aattgctaaa tacgattacc ttggtttgtc tcacaaaatt gatgaaaaaa gcgcctttgg
     1861 tttcactgct attcgctttg gagtagatga tattccaaac actacgcaat taatcgacaa
     1921 ccaaggaaat attgactacg atagaattac cacttttacg gcagccgact atggtttttt
     1981 attctcctat gcgcgtaaat tacctatcaa aggattgagt tttggtggaa attttaaagt
     2041 aatttaccgc caagttggaa aatttgcaaa aagttgggga tttggtttag atgccggttt
     2101 gcaataccaa ctcaacgatc attggaagtt tggagcagtt gttagagatg ttacttcaac
     2161 tttcaacgct tggattttta ctcttgacga ccaaacaaag gaagtgttta cacaaacagg
     2221 aaatgaaatc cctaaaaatg ggctggaatt aacattacct cgcattatct taggagcata
     2281 caccaaatat cctattggaa aaaaaggaat ttattttgca agtgaactgg atttggattt
     2341 cacaactgat ggaaaacgta atacccttat ccgctctaaa gtgttttcta tcgaccctaa
     2401 atttggaatt gaatttggct tcaaacactt cgtatccatt cgagcgggga ttaccaacat
     2461 gcaatttact aaagatttta acgacaaaaa gaaatttaat attcaaccca acatcggaat
     2521 tggacttgga tttaaaagtg tgcgcctaga ttatgcattt accgatattg gtgatgcatc
     2581 cgttgcactc tattctcacg tcatatcttt acgtgtaggt ttaaacaaac caaaaaatgc
     2641 tgtaaaaaaa ccaaaggagt cggagaaacc aaaaacatct gaatagaaat tagataaaat
     2701 gaaattaact cgccacatat tattgattgt ttgctactta tttttgctag gtaattgggc
     2761 aaatgcgcaa acttatggaa atgaatggat agattataac caaacttatt atagttttaa
     2821 aatctatcaa acaggcttaa aaagaatcaa ttacgacgac ctatacacag ctggcgtacc
     2881 gctaggcaca tttacacacg aaaacattca attgtttgga agagaaaaac aagtgcctat
     2941 ttgggttgaa accaatggtg atgggacttt tgattctggc gattacattt tattttatgc
     3001 tgaaaaaaac gatggttggt tggatactac attgtatcta gaccctacaa aatcagcaaa
     3061 cccaaccatc agtttattta acgatacgat taactatttt tttacgtgga acaaccaaac
     3121 aaacaatctc cgttttacag ttgaagacgg cagtgattat accaattaca cacccattga
     3181 ttttgtttgg aataaatttg aacaaggata tgctaatgcc taccttgaag gtgaaacttt
     3241 gcaatcgtta atttcaagct ccttttatgt ttcaggagaa ggttacggac taggttctgt
     3301 aaacggtgta aatggttata atttgccttt agttgcatca acgccttttc catatacaaa
     3361 tacaggtgct ccacaggcac tcttcaaagg aaaagtttca acaactgcca gtgcagccat
     3421 acaaaatgct ggcgacaata accaccacac tcaattctca attaacggac aagtggtttt
     3481 tgaccaaatg ggatatggac actttcaatt caatgccaat gtgccgtttt ctacctcctt
     3541 gctaggaaca ccaacaacac cacttcaatg gtcaattgta ggagatttgc ctgttgcaac
     3601 aatgtatgaa tccctaactt attggtcgat tgaatatcca agaattccaa attttcaagg
     3661 tcttactaag tttaaagtaa acgttccaaa tagcaatttc tctaaaacac gcgttgattg
     3721 cagtaacttt tccatcacca atcctgttgt ttttagtttt ggtagcacac caagtaaaat
     3781 cacccctata actgatggtg ccaatcaagt attagtatta cctataaata cccaagattc
     3841 tgtatcaacc ataattgccg aaagtctgaa ttcagcacaa acaattactg cgttcaattt
     3901 agtgaatgga acaggaaaat ttacagattt cgcaagtctt aatcacgaaa aagcatacat
     3961 cattgtttat cctccgaaat taactgctgg agcaaacgat tataaattgt accgccaaag
     4021 tattgctgga ggtgcgcaca atgtaattat ggcaaacgtg gaagaattat atttgcaata
     4081 tggtggcgga attggaaaac acattaatgc aatccgacga tttgcacatc aaacctacaa
     4141 cctttctaca gaaaaaccta ttgcattgtt cttactagga aaagcattgt atcccggata
     4201 ttcaagattt aatcctacca attatagtaa taacttagta cctacgtttg gaactcctcc
     4261 atcggatatc ttgattacat ctaaccttct gggaacaacc aaatgggctc ctttaattcc
     4321 aactggtcga ctttcggtag attctgatgc tggagttgct aattatttgg aaaaaattac
     4381 agattacgaa acacaccaaa atcaaagtga tgtgtatgac tcgcctaata aaga
//
LOCUS       DAOQVQ010000159         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22058_length_4417_cov_4.369326, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000159 DAOQVQ010000000
VERSION     DAOQVQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22058_length_4417_cov_4.369326"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1012
                     /gene="obgE"
                     /locus_tag="PKN22_06000"
     CDS             11..1012
                     /gene="obgE"
                     /locus_tag="PKN22_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNS42293.1"
                     /translation="MAGSNFIDYVKIFCRSGKGGAGSLHFHREKFITKGGPDGGDGGR
                     GGHVYIKGNKNLWTLFHLKFVRHVKAGHGGDGGSARSTGADGEDKYLEVPLGTVVKDK
                     ETGEVLFEITEHDEVKILAKGGKGGLGNWHFRSATNQTPRYSQPGMPIEEKDIILELK
                     VLADVGLVGFPNAGKSTLLSVLTSAKPKIADYPFTTLKPNLGIVAYRDFQSFVMADIP
                     GIIEGAAEGKGLGHYFLRHIERNSTLLFLIPADADDIKREYDILLDELRRYNPEMLDK
                     DRLIVISKSDMLDDELKAEMKVELDKAFKGTPYMFISSVTQQGLQELKDKLWQMLNS"
     gene            1160..3310
                     /locus_tag="PKN22_06005"
     CDS             1160..3310
                     /locus_tag="PKN22_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNS42294.1"
                     /translation="MKKLVLFVVTAMLMLPASVKANEGMWFLMFLERLNHRDMQKMGL
                     QLTADEIYSINNHSLKDAIVQFNGGCTAEMISKDGLVLTNHHCGYDKIAELSTPENNY
                     LKDGFWAADRSKELKPKSLSVRFFVRMDDVTKRILSKVNPSMTEAEREAAINKETALI
                     QKENDEGGKYTVSVRPFFQGNEYYYFVYQDYTDVRLVGTPEESIGKFGGDTDNWEWPR
                     HTGDFALFRVYADANGQPAEYSPNNVPLQPKHYLPINIGGVKENDFAMILGYPGRTNR
                     WMPASGIEQNVKFAYPAWVEGSKMGMDKLKVHMEQSAQVNLMYASKYASLANYWKNRQ
                     GMIDALTQFGTAKTKTAQEAKFNKWANKKENKAKYGNVVATINNYYALTNEKAKHDNY
                     LMTLLRSSSYGTIARSLGRQLEAYANANEEKRKQMAPAIAEMTDEFFTEVYIPAEKDV
                     LAAQLNLYATKATAHGIAPFVAEIGKSNNNDFTAYVNAAFELSIFSSADKIKAFLLVP
                     NQEAVANDPLVKLSNELLNHYLAKSEAIEKAQNDYNGAFRLLVEGLRESKIATILYPD
                     ANSTLRLTYGKVRALPADKRNDAKVNNYTTMEGLVKKYKKGDAEFDLPTKILDMQKAR
                     NYGRYADKDGTLHVNFLTDNDITGGNSGSPVLNGKGELIGLAFDGNIEAMAGDVIFDP
                     KLQRTINVDIRYVLWVIENFSGAKHIVDEMTIVK"
     gene            complement(3381..3833)
                     /locus_tag="PKN22_06010"
     CDS             complement(3381..3833)
                     /locus_tag="PKN22_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42295.1"
                     /translation="MKYKQKITELTFDKNPKALEQYLQSLPLTEQVLFLKDFKVFTQQ
                     NMFKSGDFSIADTFKKLAKNIDAYEKAVIEELKAEEDYKKALEAQEAQLQKMEAAALG
                     AKKYIMNCIINNESNATDMLELSKKIIASEKETGFYDENVWKPILHLI"
     gene            complement(3823..4272)
                     /locus_tag="PKN22_06015"
     CDS             complement(3823..4272)
                     /locus_tag="PKN22_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42296.1"
                     /translation="MKIKTIKIKVTQDNQDFDFSEFFTGSNTIYSTSAQLIQEEDGLY
                     WHAFFTYQQQGGSYLFSKKHGISNEKKAPDGFEEALLEFVNTKTNVSRRVKNSIKGDS
                     DIVYTFEKVEDFIILKSLGKGSINKEKEFLLNVLEFIKKFKETKNEV"
BASE COUNT         1470 a          683 c          822 g         1442 t
ORIGIN      
        1 aaaacaaatt atggcaggat cgaatttcat tgattacgtt aaaatatttt gtcgctcggg
       61 taaaggtggt gcaggttcgt tgcattttca ccgcgaaaaa ttcatcacca aaggtggccc
      121 tgacggtggt gatggtggtc gcggcggaca cgtatatata aaaggaaaca aaaatctttg
      181 gacacttttt cacttaaaat ttgtgcgtca cgtaaaagcg ggtcacggtg gtgacggagg
      241 aagcgcaaga agtacaggag ctgatggcga agataaatat ttagaagtgc cacttggaac
      301 tgttgttaaa gataaagaaa caggtgaagt tttatttgaa ataacggaac acgatgaggt
      361 taaaatcctt gcaaaaggag gaaaaggagg attaggaaac tggcatttta gaagtgctac
      421 aaatcaaacg ccacgctatt cacagcctgg tatgcctatt gaagaaaaag atattatttt
      481 agaattaaaa gttttagcag atgttggttt agtaggtttt cctaatgcag gaaaatcaac
      541 tttgttgtcg gtcttaactt cagctaaacc aaaaattgct gattatccgt ttactacttt
      601 aaaaccgaac ttaggaattg tagcgtacag agatttccaa tcatttgtca tggcagatat
      661 tcctggtatt attgaaggtg ctgctgaagg taaaggttta gggcattatt tcttacgaca
      721 cattgagcgt aattccactt tattgttttt aattccagct gatgcggatg atattaagag
      781 agaatatgat attttattgg atgaattacg tcgttataat ccagaaatgt tagacaaaga
      841 tcgtttaatt gttatttcaa aatctgacat gttagacgat gaacttaaag cggaaatgaa
      901 agtggagcta gacaaagctt ttaaaggaac accttatatg tttatttcat cggtaaccca
      961 acaaggtctg caagaattga aagataaatt gtggcaaatg ttgaattcat aattcaataa
     1021 taaaattcca acaaagcatc tctatcgggg tgcttttttc gttaaaatta aattaaaagt
     1081 gcgacttttc ttttgcgaaa ttttattcaa aattaatttt gagtatcttc gccctatcaa
     1141 atcaaaaatt agattttcaa tgaaaaaatt agtactattc gtagttactg ctatgctaat
     1201 gttaccagca tcagtaaaag ctaacgaagg aatgtggttt ttaatgttct tggagcgttt
     1261 gaatcaccgc gacatgcaaa aaatgggctt acaattaaca gccgatgaaa tttacagcat
     1321 caacaatcac agtttaaaag atgcaatcgt tcaatttaat ggaggatgta ctgctgaaat
     1381 gatttcaaaa gacggactag ttttaaccaa tcaccactgt ggttacgaca aaattgctga
     1441 actatcaact ccagaaaata actatttaaa agatgggttt tgggctgcag atagaagcaa
     1501 ggaattaaaa ccgaaaagtc tttctgttcg tttcttcgtt cgtatggacg atgtgacaaa
     1561 aagaattctg tctaaagtaa acccttcaat gactgaagca gaaagagaag cagctattaa
     1621 taaagaaaca gctttaattc aaaaagaaaa tgacgaaggt ggaaaataca cagtttctgt
     1681 tcgtccattt ttccaaggaa atgaatacta ttattttgta taccaagatt atacagatgt
     1741 gcgtttggta ggaactccag aggaaagcat aggtaaattt ggtggagaca ctgataactg
     1801 ggaatggccg cgtcatacag gtgattttgc tttgtttaga gtgtatgctg atgcaaacgg
     1861 acaaccagca gaatattctc caaacaatgt tccattacaa ccaaaacatt atttacctat
     1921 caatattggt ggagtaaaag aaaatgattt tgccatgatt ctagggtatc caggtagaac
     1981 taaccgttgg atgccagcta gtggaattga gcaaaacgta aaatttgctt atccagcttg
     2041 ggttgaaggt tcgaaaatgg gaatggataa gttaaaagtt catatggaac aaagtgctca
     2101 agtgaattta atgtatgcat caaaatacgc ttcattggct aattattgga aaaaccgtca
     2161 aggaatgatt gatgcgttaa cgcaatttgg tacagctaaa acaaaaacgg ctcaagaagc
     2221 gaaatttaat aaatgggcaa ataaaaaaga aaataaagca aaatacggaa atgtagtggc
     2281 aactattaat aattattatg ctttaactaa tgaaaaagcg aaacacgata attatttaat
     2341 gacattgttg cgttctagtt cttacggtac aattgcaaga agcttaggtc gtcaattaga
     2401 ggcttatgcc aatgccaatg aggaaaaaag aaaacaaatg gcacctgcaa ttgcagaaat
     2461 gactgatgag ttttttacag aggtttatat tcctgctgaa aaagatgttt tagctgctca
     2521 attgaactta tatgctacta aagctacagc tcatggaatt gccccttttg ttgctgaaat
     2581 tggaaaatca aataataatg atttcactgc ttatgtaaat gcggcatttg aattgagtat
     2641 cttctcttct gcggataaaa tcaaagcctt tttattggtt ccaaatcaag aagcagttgc
     2701 aaatgaccca ttggtaaaat tatctaatga gttgctaaat cattacctag caaaatcaga
     2761 agcaattgaa aaagcgcaaa acgattataa tggggcattc cgtttgttag tggaaggttt
     2821 gagagaatca aaaattgcta ccattttgta tccagatgcc aattcaacgt tacgtttaac
     2881 ttatggaaaa gttcgtgctt taccagctga caaacgcaac gatgcaaaag tgaacaacta
     2941 tactacaatg gaaggtttgg taaaaaaata caaaaaaggc gatgctgaat ttgatttacc
     3001 aaccaaaatt ttagatatgc aaaaagcacg taactacggt cgttatgcag ataaagacgg
     3061 aacgttacac gtgaatttct taaccgataa cgatatcact ggaggaaatt caggttcacc
     3121 agttttaaac gggaaaggtg aattgatcgg tttggcattt gatggaaaca ttgaagctat
     3181 ggctggagac gtaatttttg acccaaaatt acaaagaaca atcaacgtag atattcgtta
     3241 cgttttatgg gtaattgaga atttctcagg agcaaaacac attgttgacg aaatgactat
     3301 tgtgaagtaa ttttcaaatc aatataaata gaaacgtccc gatttaacgg gacgtttttt
     3361 ttatggttta cttttgaata ttatattaaa tgtaatatag gtttccaaac gttttcatca
     3421 taaaaacctg tttctttttc tgaggcaatt atttttttag ataattctaa catatctgtt
     3481 gcattagact cgttatttat tatacaattc ataatatact tttttgcacc taaagcggct
     3541 gcttccattt tttgtagttg tgcttcttgt gcttctaatg cttttttata gtcttcttcg
     3601 gcttttagtt cttctataac ggctttttca taagcgtcta tgttttttgc taatttttta
     3661 aaagtatcag caatactaaa atcgccgctt ttaaacatgt tttgctgtgt aaatacttta
     3721 aaatctttta aaaacagtac ttgctctgtt agaggtaaac tttgtaaata ctgttctagg
     3781 gcttttgggt ttttatcaaa agtgagttcg gttatttttt gtttatactt cattttttgt
     3841 ttctttaaac ttttttatga attctaatac gtttaacaga aattcttttt ctttgtttat
     3901 gcttcctttt cctaggcttt ttaaaattat aaagtcttca actttttcaa aagtataaac
     3961 aatatctgaa tcccctttta tactattttt tacccttcta ctaacatttg ttttagtatt
     4021 tacaaattct aaaagtgctt cttcaaagcc atctggtgct tttttttcgt ttgagatacc
     4081 atgtttttta gagaacaaat aagagccacc ttgttgctga tacgtaaaaa acgcatgcca
     4141 gtacaaacca tcttcttctt gtattaactg ggctgaagta gaataaatag tattgctacc
     4201 cgtaaaaaat tctgaaaaat caaagtcttg attgtcttgt gttactttta tttttatggt
     4261 ttttattttc attgttttat gttttagtta ctacctctct ggttgcagct atctttctat
     4321 ctgcatactc caccacctct tttatgacca acttttgcac cgcttttata aacacttcca
     4381 tgtaagcata taacggaacg ccttgcgaag agggaag
//
LOCUS       DAOQVQ010000160         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22148_length_4404_cov_3.224189, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000160 DAOQVQ010000000
VERSION     DAOQVQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22148_length_4404_cov_3.224189"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3509
                     /locus_tag="PKN22_06020"
     CDS             <1..3509
                     /locus_tag="PKN22_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42297.1"
                     /translation="YYVTQSNDEYYSHSIDDITGGIYDYTDPSPGKKSNVFFGATKEK
                     SDGSRLAFVTNGYGIFIFNVTTSGFNFSHVIPLPNGNFNPYNIRSEFEVAELSSGNYR
                     IAAPFLSSLTGTYQGLFTAELTPSGVVVPNSVKEFYTDYYSQGGVSLSSKFRGVEFSE
                     DGNMLYVTSTTNAISPNSLFYYDFLNPTSSLNPITLPSPLNQDLFQYSQIERGPLNKL
                     FLATGNGLYTLNNMNNPSSSIVLSSSFTYNQNHEGEPLPNTLGMYSLPDQIDGMDYVN
                     LYEINLACCIDSRNYQIKKHNAISGTWNGSSPIAPGSSSITIKEELRIPAGVSLTINN
                     MTLEFAPGARLVIENGTSGSQGGKLILNGTTLTVDDRCDTNTMWLGVEVWGNTTAAQG
                     SVLNSSQGVFRMYNNSKIEHAEIGVLISRRNETNGVPISTSYNGNFNGGVFIAENSSF
                     YNCWIGTMFQSYGVAATNLSRISSCNYTWNGQLRDLTKTPHVHLGIVQCNKIYVTATK
                     FNQNTPSIYTNKNKWGIGILALNSDFAVSSSCSTILPIGQDCSEANTIRSEFNNLTTG
                     VGVLNGNNKPFTVLRNEFTNCLYGVTSATAKNQKISRNNFFIHEDNAIQTWGISVKNG
                     TGYEIAENKLQSMTGAIDQTYGIIIDNSGEDKNEVYKNFFVDLYVGTQAQNVNGKVYV
                     PGTNNISQGLRYNCNTFKSFIEKADIAVAVNSRIDYEQGRVGGSSINEARNSNTARNE
                     FSYTPLATDIFLSTGSQQVNYYHLANPGHAPLTYTNTPPIAVYPALQTWGSAPLYTDD
                     FTCLTKYPSTWPPIVFSFPFGNSIKIDSLYNLVDGGKSSYLLGLIETQPTSSFTFNEL
                     MDASPYLSDTVLTNYIYSKASNNYLKDVLIQNSKLTGVVWDTLKASSRPSGLKDNIAW
                     YQGGVSAMQELYEAIKINEDRLMDAQQVYIYSILDDTTLVSKNDTLVKYLELCRGETY
                     KKQLLNVYSSMQNQTKFDSLLTVITLPKYMKEYYKIVYQLDKKGDWITAIEQDSTILN
                     DLIALSQKDNVEIASNAATILDIYYGNYSNFELLPLVNTPKSLVIHNGNSSEENVEKQ
                     SGFMVFPNPSHGTVTVQFSEMDVQTAIVQLVDLSGKELFTQKFTNSKNAELDLSSIKK
                     GTYLLKIEVDGHLTSSQLLILE"
     gene            complement(3679..>4404)
                     /gene="accD"
                     /locus_tag="PKN22_06025"
     CDS             complement(3679..>4404)
                     /gene="accD"
                     /locus_tag="PKN22_06025"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNS42298.1"
                     /translation="IEKLFWVCDRCGHHGRIGSDDYFKIIFDDGKFKELNPKLISGDP
                     LEFEDTKKYKDRVTSTIKKTGLNDAVKTAVGKLDGHDFVIACMDFDFIGGSLGSVVGE
                     KIARAIDHAIKIKAPFMIISKSGGARMMEAGYSLMQMAKVSGKLGQLSDAKLPYISLL
                     TNPTTGGVTASYAMLGDLNIAEPEALIAFAGPRVVKETIGRELPDGFQTSEFVLEHGF
                     LDYIVDRSELREKLALTLKLFRD"
BASE COUNT         1520 a          746 c          740 g         1398 t
ORIGIN      
        1 cttattatgt cacacaaagt aatgatgaat actattcaca ctcaattgat gatattacgg
       61 gtggtattta tgattatacg gatccaagtc caggtaaaaa atcgaatgtt tttttcggtg
      121 caaccaagga aaaaagtgat ggtagtagat tggcttttgt aacaaatggt tatggaattt
      181 ttatctttaa tgtaactact tcaggattta atttttcaca tgtaattcct ttaccaaatg
      241 gaaattttaa cccttataat atacgctccg agtttgaggt tgctgaattg tcttcgggta
      301 attatcggat tgcagctcca tttttatcct ctttaacagg gacatatcaa ggattattta
      361 ctgctgaatt aacaccatct ggagtagtcg tacctaactc ggtcaaagaa ttttatacag
      421 attattatag tcaaggaggt gtttctttat cttcaaaatt tagaggtgta gaattttcag
      481 aagatggaaa tatgctttat gttactagca caacaaatgc aatttctcca aattcattat
      541 tctattacga ttttcttaat cctacatcct cacttaatcc aataacatta ccttctcctc
      601 tcaatcaaga tttatttcaa tattcgcaaa ttgagcgagg gcctttaaat aaattatttt
      661 tagctactgg aaatgggcta tatacgttga ataatatgaa taatccttct tcttcaattg
      721 tattatcatc ttcctttaca tataatcaga atcatgaagg agagccctta cctaatacat
      781 taggaatgta ttcacttcct gatcaaatag acggtatgga ttatgtgaat ttatatgaaa
      841 taaatttagc atgttgcatt gattcacgta attatcaaat taaaaaacac aatgcaatta
      901 gcggcacatg gaatggttca agtccaatag ctcccggaag ttcttctata actatcaaag
      961 aagagctacg catacctgct ggtgtaagtc ttacaataaa caatatgaca ttggaatttg
     1021 cgcctggtgc acgtttagtt attgaaaatg gaactagcgg ttcgcaagga ggaaagttaa
     1081 ttttaaatgg cacaacacta actgtggatg acaggtgtga taccaacact atgtggttgg
     1141 gtgtagaagt ttggggaaat actacagctg cacaagggag tgtattaaac tcatcacaag
     1201 gagtttttag aatgtataat aattctaaaa ttgaacatgc tgaaatcggt gttttaatct
     1261 cacgaagaaa tgaaacaaat ggtgtgccta tttctacgtc atacaatggt aatttcaatg
     1321 gaggtgtgtt tattgcagaa aatagttctt tttacaactg ttggataggt accatgtttc
     1381 aaagctatgg tgtcgctgct accaatttat caagaatcag tagttgtaat tacacatgga
     1441 atggacagtt aagggatcta acaaaaaccc cacatgttca tttaggaatt gtgcagtgta
     1501 ataagattta tgttacagca acaaaattta accaaaacac cccttctatc tataccaata
     1561 aaaacaaatg gggaattggt atcttagcat taaatagtga ttttgctgta agcagttctt
     1621 gctctaccat tcttccaatt gggcaagatt gttcagaagc aaataccatt cgttctgaat
     1681 ttaacaatct aaccacaggt gtaggtgttt taaatggtaa taacaaacca tttaccgttt
     1741 tgagaaatga atttacaaat tgcctctatg gtgttacttc ggcaacagcc aaaaatcaaa
     1801 aaattagtag aaacaatttc tttattcacg aagacaatgc tatacaaaca tggggtatca
     1861 gtgtaaaaaa tggaacagga tatgaaattg cagaaaacaa attgcaatct atgactggag
     1921 caattgatca aacttatggt atcattattg ataattcagg agaggataaa aacgaagtat
     1981 ataagaattt ctttgttgac ttgtatgttg gtacacaagc gcaaaatgtc aacggaaaag
     2041 tttatgttcc aggtactaat aacatttctc aaggattgag atacaattgc aatacgttta
     2101 aatcttttat agaaaaagca gatattgcag tggctgtaaa tagtagaatc gattatgaac
     2161 aaggtagagt tggtggttca agtatcaatg aagcaagaaa tagtaacacg gcaagaaatg
     2221 agttttcata cacgccttta gctaccgata tttttctttc aacaggtagt cagcaagtta
     2281 attactatca tttggcaaac cctggtcatg caccacttac ctataccaat actccaccga
     2341 ttgctgtgta tcctgctttg caaacatggg gaagtgcgcc tttatatact gatgatttta
     2401 cttgtttaac aaaatatcca tcaacatggc cgccgattgt gttttcgttc ccatttggaa
     2461 attctataaa aattgatagt ttgtataatt tggtagatgg aggaaagtca tcatacttac
     2521 ttgggttgat agaaacacaa cctacaagct cgtttacatt caacgaatta atggatgcat
     2581 ctccttacct aagtgatacc gtgttaacca attacattta ttcaaaagca tcaaataact
     2641 acttaaaaga tgtattgata caaaatagca agttaacagg tgtggtttgg gatacactca
     2701 aagcatcatc cagaccatcg ggcttaaaag ataatattgc ttggtatcaa ggaggtgttt
     2761 ctgctatgca agaactttat gaggcaataa aaattaatga ggatagatta atggatgcac
     2821 aacaagtgta tatttattct attttggatg atacaacact ggtttcaaaa aatgatacac
     2881 tcgttaagta tttggaacta tgtagaggcg aaacgtataa aaaacaattg ttgaatgtat
     2941 atagttcaat gcaaaatcag acaaaatttg attcgttgtt aactgtcatt acattaccaa
     3001 agtacatgaa agagtattac aaaatagtct atcaattaga caaaaaaggt gattggataa
     3061 ctgctataga acaagatagc actattttaa atgatttgat tgcgttgtca caaaaagaca
     3121 atgtagaaat agcaagtaat gctgcaacaa tattggacat ctattatggt aattactcta
     3181 attttgagtt gctaccgttg gtgaatacac cgaaatcact agtgatacac aacggaaatt
     3241 ccagcgaaga gaacgtagaa aaacaaagtg gttttatggt gtttccaaat ccttctcatg
     3301 gcactgtaac agttcagttc tcagaaatgg atgtgcaaac tgcaattgtt caattagtgg
     3361 atttatcagg taaagaacta tttacacaga aatttaccaa ctcgaaaaac gcagaattgg
     3421 atttgtcaag tattaaaaaa ggaacgtatc tattaaaaat tgaagtagat ggtcacttga
     3481 cttcttctca attgttgata ctggaataat agtaaataca ctcatttatc aaccgacttt
     3541 cagaaatgga agtcggtttt tttaattcct tttctaatta ctttaatgga attaatctta
     3601 atatttcctc taacaagtaa aacaaaaaag gcatccaaaa ctttagatac cttctcattt
     3661 atgtttaact aatttctgct aatctctaaa taattttagt gttaatgcca atttttctct
     3721 caattcactt ctatcaacaa tgtaatctaa aaagccatgt tctaaaacaa attcagaagt
     3781 ttggaaacca tctggcaatt cacgaccgat tgtttctttt acaacacgag gtccagcaaa
     3841 agcaatcaac gcttcaggtt ctgcgatatt taagtcgccc aacatagcgt aagaagctgt
     3901 aacaccaccc gttgttggat tggtaagcaa tgaaatatac ggtaatttag catctgacaa
     3961 ttgacctaat ttaccagaca cttttgccat ttgcatcaaa gaataacctg cctccatcat
     4021 acgtgctcca cctgattttg aaataatcat aaacggagct ttaattttaa tagcatgatc
     4081 aattgcacgc gctatttttt caccaacaac agagcccaat gaaccaccga taaaatcgaa
     4141 atccatacaa gcaatcacaa aatcatgtcc atccaattta cccacagcag ttttaacagc
     4201 atcatttaaa cccgttttct ttattgttga tgttactctg tctttatact tcttagtatc
     4261 ttcaaactct aaagggtcac cagaaatcaa tttagggttc aattctttga atttcccatc
     4321 atcaaagata attttaaaat aatcgtcgga accaattcta ccatggtgtc cacaacgatc
     4381 acagacccaa aacaactttt ctat
//
LOCUS       DAOQVQ010000161         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22474_length_4360_cov_3.193031, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000161 DAOQVQ010000000
VERSION     DAOQVQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22474_length_4360_cov_3.193031"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKN22_06030"
     CDS             complement(<1..429)
                     /locus_tag="PKN22_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNS42299.1"
                     /translation="MQRAPIMKSRVYILFALSAALFGQSCAKKMTEGTVILPGHNSED
                     AVEVLDSIQKNTPYFLSSKIDTRYTDNKQNISFKTSLRMSKDTSIWALFTYAGVPLIT
                     NYITKDSIQISNKKEKCYIKDSRNFIARNFKIDLSLGDLQS"
     gene            complement(401..2104)
                     /locus_tag="PKN22_06035"
     CDS             complement(401..2104)
                     /locus_tag="PKN22_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42300.1"
                     /translation="MKKILLIALIGIISVACATKKNSNQASIHSQASFSYIEAFHKGM
                     RLKIKGDSDAAIEAFQKCLNMRKDDDAVFYALSQLYVAKKDYQQAVSMIESANKIDPK
                     NIWYTEQLANLNYGLKQYDKAAIYFKKLVEYEPKNIDWLYGYGDCLLKNGKVEEAISI
                     INKAEVVLGKNPSLSIEKFNLYKSIKKDKEAIAELESILKEYPQEPQVIATLADYYFS
                     KGNVEKGVGYLTQLVAADPENGRAHLALGEIYRKQKKVDEAFEQFLAAFKCEDVDVDT
                     KMSLLIELQESPLSSHPKTLELVDLMVDLHPESAKSNSIKGDYLLAKGQEKEALVYYK
                     KALDTEKSQFPIWNQVMVLEYQYLMWNDLFEDSKQCLNYFPNLPIVYLLNGTSSIELN
                     KPNEAIDVLTTGKAFVINNKGMEAEFMAQIGEANFMLKNIKEGKKWYEDAIKLDPSSN
                     HLKNNYANRLAIAKIDFDLAFSLINQSISNSPTISQYYDTRGWIYFQQAKYSQALEDY
                     LKAFELESTNALYIEHLGDVYSKLNQKDKALQYWQKAVELGVEKDVLKKKIATGTYHE
                     E"
     gene            complement(2101..3111)
                     /locus_tag="PKN22_06040"
     CDS             complement(2101..3111)
                     /locus_tag="PKN22_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS42301.1"
                     /translation="MNIIIPMAGRGSRLRPHTLTIPKPLVPVGGKPIVHRLVESIAEV
                     LNNKIDTISFVIGDFGQEIEEELIKVAEKLGAKGSIHYQTEPLGTAHAVLCAKENLVG
                     PTVVAFADTLFKADFKINPEDDGILWVKQIEDPSAFGVVKLNDKFEITDFVEKPKEFV
                     SDLAMIGIYYFREGEKLRTELEYLIDNNVIKSGEYQLPDALRRLTEAGYKFKPGKVIE
                     WLDCGNKEVTVFTNQRVLEYDKEDGKLYYASNAKIDNSTIIQPCFIGDNVVIDNCIIG
                     PHVSIGANSNIANSIIKNTIIQDSSSVKNAIVANSMIGSHAHYQASSKDLSIGDYTTI
                     KE"
     gene            complement(3124..3558)
                     /gene="dut"
                     /locus_tag="PKN22_06045"
     CDS             complement(3124..3558)
                     /gene="dut"
                     /locus_tag="PKN22_06045"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNS42302.1"
                     /translation="MKVKIINQSKHSIPKYETIFSAGMDVRANLNEPITLEPFQRALI
                     KTGLFIELPQGYECQVRPRSGLAFKNGITVLNSPGTIDADYRGEIGVILINLSNEAFV
                     VNDGERVAQLVFAKYEQAEFVEVDVLSDTQRGTGGFGSTGVK"
     gene            3777..4229
                     /locus_tag="PKN22_06050"
     CDS             3777..4229
                     /locus_tag="PKN22_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNS42303.1"
                     /translation="MGATKYEAYTERQIQFANFFKSIAHPARFAAIESLIEAGGDELT
                     FDEIFKNINLAQSTKSVHLKQLIEAGIIKTKLTKNRNKSCLKYRLNKTAFEFLEKLIN
                     HINDKVDLNENERLSALKGFYQNNINLYRLNLFKLSPNTYQINSSESN"
BASE COUNT         1427 a          787 c          582 g         1564 t
ORIGIN      
        1 gctttgtaag tcacccaaac tcaaatcaat cttaaaattt cgggcaataa aattgcgact
       61 atctttgata taacactttt cttttttatt ggaaatttga atagaatctt ttgttatata
      121 atttgtaatc aaaggaactc cagcataagt aaacaaagcc caaatagatg tgtctttact
      181 catgcgtaaa gaagttttaa agcttatatt ctgtttatta tctgtgtatc ttgtatcaat
      241 ttttgaagaa aggaaataag gtgtgttttt ttgaatacta tctaatactt caacagcatc
      301 ttcgctatta tgtcctggta aaataacagt gccttcggtc attttttttg cacaagactg
      361 accaaacaat gctgcactaa gggcaaataa tatgtaaacc ctactcttca tgataggtgc
      421 ccgttgcaat ttttttcttc aaaacatcct tttcaacacc caattcaaca gctttttgcc
      481 aatattgaag agctttatct ttttggttta atttgctgta tacatctcca aggtgttcaa
      541 tgtatagcgc attggtgctt tccaattcaa aagcttttaa atagtcttca agggcttgag
      601 aatacttggc ttgttgaaaa taaatccatc ctctcgtatc gtaatattgc gatatggtgg
      661 gagagttaga aattgactga tttattaagc taaaagccaa atcaaaatca atctttgcaa
      721 ttgctaaacg attagcatag ttgtttttca gatgattgct tgatggatca agtttaatcg
      781 catcttcata ccacttttta ccttctttaa tatttttaag cataaaattt gcttccccaa
      841 tttgagccat aaattcagct tccatccctt tgttgttgat aacaaatgct tttcctgtag
      901 tcaatacatc aattgcttca ttaggtttat tgagctctat actactcgtt ccattaagta
      961 aataaacaat aggtaaattc gggaaatagt ttaaacattg cttgctatct tcaaacaaat
     1021 cattccacat tagatattgg tactctaaaa ccattacttg attccaaata ggaaattgag
     1081 atttttcagt atctaatgct tttttataat aaaccaatgc ttctttttct tgaccttttg
     1141 ccaataaata atctcctttt attgaattgg atttagcact ttctggatgt aagtcaacca
     1201 ttaaatcaac taattctaat gtttttggat gtgatgacaa agggctttct tgtagttcta
     1261 tcaataaact cattttcgtg tctacatcaa catcttcgca tttaaaagct gctaaaaatt
     1321 gttcaaaagc ttcatctacc tttttttgtt ttctataaat ttctcctaaa gctaagtgag
     1381 ctctgccatt ttcaggatca gctgcaacta attgagttaa gtaaccaact cctttttcaa
     1441 catttccttt actaaaataa taatctgcca gtgttgcaat aacctgaggt tcttgagggt
     1501 attcttttaa aatagattct aattcagcaa ttgcttcttt atctttctta attgacttgt
     1561 ataaattaaa cttttcaatt gacagacttg gattttttcc taaaacaacc tcagctttat
     1621 taataattga aattgcctcc tcaactttac catttttcaa caaacaatca ccatagccat
     1681 ataaccaatc gatattctta ggctcatatt ctactaattt tttgaagtaa atagcagctt
     1741 tatcgtattg ttttagtcca taattcaaat ttgccaattg ttcagtatac caaatatttt
     1801 ttggatctat tttattagct gactcaatca tggatactgc ctgctgataa tcttttttag
     1861 caacatacag ctgagataac gcataaaaaa cagcatcatc atctttacgc atatttaaac
     1921 acttctgaaa tgcttcaatt gcagcatcag aatctccttt tatttttaag cgcatccctt
     1981 tatgaaatgc ctcaatatag gaaaaacttg cttgtgaatg tatgcttgct tgattactat
     2041 tttttttagt tgcacaagcc acagatatta tcccaattaa agctattaga agtatttttt
     2101 tcattctttt attgttgtat aatcacctat acttaaatct tttgatgatg cttgataatg
     2161 cgcatgcgat ccaatcattg aatttgcaac tattgcattt ttaacagatg aactatcttg
     2221 aataatggtg tttttaatta tcgaattcgc aatattacta tttgctccaa ttgatacatg
     2281 tggtccaatt atacaattat ctatcaccac attatcgcca ataaagcaag gttgaattat
     2341 cgttgaatta tcaattttag cattcgaagc ataatataat tttccatctt ctttatcgta
     2401 ctctaacaca cgttgattgg taaaaactgt tacttctttg tttccacaat ccaaccattc
     2461 aataactttg cctggcttaa atttatatcc tgcttctgtc aatcttctca aagcatctgg
     2521 caattgatat tcaccacttt ttataacgtt attgtctatc aaatattcta attctgttct
     2581 taatttttca ccttctctaa aataataaat cccaatcatt gcaagatcgg acacaaattc
     2641 tttaggtttt tcaacaaaat cagtaatctc aaacttatca tttaatttca ccaccccaaa
     2701 tgccgaaggg tcttctattt gtttgaccca taaaatacca tcatcttctg ggttgatttt
     2761 aaaatctgct ttgaataatg tatcagcaaa agcaactact gttggtccaa ctaaattttc
     2821 ttttgcacac aaaactgcat gtgcagtccc taatggttcg gtttggtaat gtatagaccc
     2881 ttttgctcct aatttttcag caacttttat taactcttct tctatttctt gtccaaagtc
     2941 accaatcacg aaagaaatag tatcaatttt attatttaaa acttcagcaa tactttcaac
     3001 caatcggtga acaattggtt ttcccccaac gggaactaat ggtttaggaa ttgttaatgt
     3061 atgaggtctc aatctactac ctcttcctgc cattggaata ataatattca tttttctaat
     3121 tttttattta acacctgtac ttccaaaccc accagttcca cgttgtgtat ctgaaagcac
     3181 atctacttca acaaattctg cttgttcgta ttttgcaaac accaactgag ctactctttc
     3241 accgtcattt actacaaaag cttcattgga taaattaatt aatattactc caatttctcc
     3301 tctataatcc gcatctattg tacccggtga gtttaaaacc gtaattccat ttttaaacgc
     3361 taagccactt cttgggcgaa cttgacactc ataaccttgt ggcaactcaa taaacaatcc
     3421 agttttaatc aatgctcgtt gaaatggttc tagagtaatt ggctcattca aatttgccct
     3481 aacatccata ccagcagaga aaattgtttc atatttagga atagaatgct tggactgatt
     3541 gattatttta actttcatga ttatgattat ttacaataac aaacaaattt aggaaaatat
     3601 ggtgatgtaa aattggggtt aatattgaaa gttgtgaggg gtgtgttgtt gaaaaagtta
     3661 aaataaatcg taaaaatcac catatcgtaa tatagcgata tagataattt ctaaaaatat
     3721 tttggaaaac taataaaatt aactatctta gtgttaaacc ttaaactttt tacaatatgg
     3781 gagccacaaa atatgaggcg tatacagaac gtcagattca attcgcaaac ttttttaagt
     3841 caattgctca tcctgcaaga ttcgctgcta ttgaaagtct catcgaagct gggggagatg
     3901 aattaacttt tgatgaaatt ttcaaaaata ttaacctcgc acaatctaca aaatctgttc
     3961 atttaaagca gctgatagaa gctggcatta taaaaactaa gctaacaaaa aatagaaata
     4021 aaagctgttt aaaatatcgt cttaacaaaa cagcatttga gtttcttgaa aaacttatca
     4081 accacataaa cgataaagtt gatttaaatg aaaatgaacg attaagtgca ctgaagggat
     4141 tctatcaaaa taacattaat ctatataggt taaatttatt taagttaagc cctaacacat
     4201 atcaaataaa ttcctcagaa agcaactaaa cattaaaata taaaaataac ctatcaaaat
     4261 ggaaacaaac cgttcaataa aatgttgggc agaagatgat cgccctagag aaaaaatgtt
     4321 attaaaaggc aaaagtgttc tatctgatgc agagctaatt
//
LOCUS       DAOQVQ010000162         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22555_length_4348_cov_4.470300, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000162 DAOQVQ010000000
VERSION     DAOQVQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22555_length_4348_cov_4.470300"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKN22_06055"
     CDS             <1..392
                     /locus_tag="PKN22_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42304.1"
                     /translation="EMDIQDNLEWLHQLSKQLFAENSYEETVMLGFSQGAATAARYFY
                     GGQSYYNRFIVWASVLPPDLDAEELFAKHQEATNNTFVVGTNDEFFSAEQQAEYCAFY
                     KNLGFDVKQFEGKHDIDIPTLKEILHD"
     gene            433..1074
                     /locus_tag="PKN22_06060"
     CDS             433..1074
                     /locus_tag="PKN22_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNS42305.1"
                     /translation="MKKILTLLIVLLSFTGFSQTESDPKADKILREFSTKVNGSTSVY
                     LEFSASIKNVDGATENNIGKGWSKANKYYATFGDFTRISNGTKIWTIIKEEKSVYVSD
                     EDDGNATINPKRMMTLWDKDYKSKYQKETQLGNETVHVINLYPKNVKSAEFHTIIVYI
                     NKANNELKKATLKGKDGSSTTYTITKLDFNVDAPDSKFTFDKSKYPGYQIIED"
     gene            complement(1146..1637)
                     /locus_tag="PKN22_06065"
     CDS             complement(1146..1637)
                     /locus_tag="PKN22_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42306.1"
                     /translation="MKKNILTIVAVCGLLVYGCGDHDGHSHEGHSHDGHSHNEQVEHA
                     DHDDHDDEAAEEIVLNEGKKWEVNAEMKPFIANAEKLLKKYIDSKGTDYKKLAEQLNA
                     ENSSLINSCTMDGKSHDELHKWLHPHLELVDELKNEDNAADAKEIVSELEHSFVTFHT
                     YFQ"
     gene            complement(1705..2880)
                     /locus_tag="PKN22_06070"
     CDS             complement(1705..2880)
                     /locus_tag="PKN22_06070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNS42307.1"
                     /translation="MKKQVQLYKNKAESVMRKHPWVFSGAIDLASEDLQDGDVVEVLD
                     SKANFLAVGHFQNATIAVRILSFDNKAIDANFFQKKIENAVQLRRNLNLFRPDNSIMR
                     LIHGEGDGLPGLIVDYYNKVAVIQCHSVGMYRAVEHIAQALHSVLKEEVIAIYNKSSD
                     TLAHKDQMEIKDGYLLGNCPTPHVALENGVKFNIDWINGQKTGFFIDQRENRALLGKY
                     AKGKKVLNTFCYSGGFSLTALKNGASLVHSLDSSAKAIQLTDDNIALNQFESHQSIVA
                     DAVEYIKNLPEQYDVIVLDPPAFAKHRDKRHKAIQGYKRLNANALRQIKPGGFLFTFS
                     CSQVIDKYLFTNTVIAAAIEVGRNVRIVEQLHQPADHPISAYHPEGEYLKGLVVYVD"
     gene            3081..3980
                     /gene="cyoE"
                     /locus_tag="PKN22_06075"
     CDS             3081..3980
                     /gene="cyoE"
                     /locus_tag="PKN22_06075"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNS42308.1"
                     /translation="MIKNKLKQYSQLTKFRLSFTVMVSALAGYLFNGGTSWSEAIVLS
                     VGGILVTGASNASNQIWERKLDAKMKRTMNRPLPSGEMSVTEAFIVVFLFLGIGAWML
                     YSINWQTAALGLFGYLSYVFAYTPTKRISRWSVLVGAIPGAVPPMIGAVAASGVYSFV
                     PGILFFIQFVWQFPHFWAIAWVANEDYKAGGFSLLPSASGRSKQSALQIAIYSLLLIP
                     FSLLPWILGWTGNVSMVIIAILGGYFYWLSYRLYITLEMKDAKKLMFASFIYLPLIQF
                     ILVFDRTESFLEHLKNAVEITFK"
     gene            3987..>4348
                     /locus_tag="PKN22_06080"
     CDS             3987..>4348
                     /locus_tag="PKN22_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNS42309.1"
                     /translation="MKQQFEQELDPEIREKLRKNLVYAAIGSIIMVFAGLISAYIVSM
                     GGAFWLKSPLPSAFYVSTVLIVISSITFSLALKAAKARNLNQLKIYISITLLLGIGFA
                     ISQFQGYKQLVEKGLYIGS"
BASE COUNT         1387 a          791 c          752 g         1418 t
ORIGIN      
        1 gagaaatgga cattcaagac aaccttgagt ggttgcacca attgagcaaa caactttttg
       61 ctgaaaacag ctatgaagaa accgttatgc ttggattttc gcaaggagct gcaacggctg
      121 cacgttattt ttatggcgga caatcctact acaaccgttt tattgtgtgg gcatctgttt
      181 tgccgcctga tttggatgca gaagaattgt ttgcaaaaca ccaagaggca acaaacaaca
      241 catttgttgt aggaaccaac gatgagtttt ttagtgccga acaacaagca gaatattgcg
      301 ctttctataa aaatttaggg tttgatgtca agcaatttga aggaaaacac gacattgaca
      361 tccccacctt gaaagaaatt ttacatgatt aatttggcac gttttatggt gaaaagaaca
      421 aaaattcaac aaatgaaaaa aatattgaca ttacttattg tgcttttgag ctttactgga
      481 ttttcacaaa cagaaagcga tccaaaggcc gacaaaatat tacgcgagtt ttctaccaaa
      541 gtaaacggca gcacatcggt ttatttagag ttttctgcat ctataaaaaa tgtggacgga
      601 gcaacagaaa acaacattgg taaaggttgg tcaaaagcca ataaatatta cgccactttt
      661 ggtgatttta cacgtatttc taacggaaca aaaatttgga ccatcatcaa ggaagaaaaa
      721 tcggtgtatg tttctgatga agacgatgga aatgcaacca tcaatccaaa acgcatgatg
      781 acattgtggg acaaagatta caaaagcaaa taccaaaaag aaacacaatt gggcaatgaa
      841 acggtgcacg ttatcaattt gtatcctaaa aatgtaaaat cagccgaatt tcacaccatc
      901 attgtttaca tcaacaaagc caacaatgaa ttgaaaaaag caactttgaa aggaaaagat
      961 ggcagttcta caacctatac catcaccaaa ttagatttta atgtggatgc ccccgattct
     1021 aaatttactt ttgataaatc aaaatatccg ggataccaga ttattgagga ttaatgattg
     1081 attataaaag aaaagagatt gattttttac atgaaatcaa tctctttttt tatgcgtgta
     1141 tttttttatt gaaaataggt atgaaaagtt acaaatgaat gttctaattc agaaacaatc
     1201 tcttttgcat cagctgcatt gtcttcattt tttaattcat ctaccaattc caaatgtggg
     1261 tgtaaccatt tgtgcaattc atcgtgactt tttccgtcca ttgtacagct gttaatcaac
     1321 gaagaatttt cggcattcaa ttgctccgct aattttttat aatcggttcc tttgctgtcg
     1381 atgtattttt ttagcaattt ttcagcattt gcaataaaag gcttcatttc ggcattaacc
     1441 tcccattttt taccttcatt aagaacaatt tcttcagcgg cttcatcatc atggtcatca
     1501 tgatctgcat gttctacttg ctcattgtgt gaatgtccgt cgtgtgaatg cccttcgtgt
     1561 gaatgtccat catggtcacc acatccatac accaacaagc cacaaactgc tacaattgtt
     1621 aaaatatttt ttttcatttc tttgatttat tatgaattga tactttacaa atataacatt
     1681 taaaaaatac aatcgtttat gattttaatc aacatacacc accaatcctt tcaagtattc
     1741 accttctgga tggtaggcag aaatggggtg atctgcaggt tggtgcaatt gttcaacaat
     1801 gcgtacatta cgtcccactt caatggcagc tgcaattacc gtattggtaa acaaatattt
     1861 atcaattact tgcgagcatg aaaacgtaaa caaaaatcca ccaggtttta tttgtcgcaa
     1921 agcatttgca ttcaatcgtt tgtatccttg aattgcttta tggcgtttat cgcggtgttt
     1981 tgcaaatgca ggtgggtcca acacaatcac atcgtattgc tctggcaagt ttttaatgta
     2041 ttcaaccgca tcggcaacaa tggattgatg actttcaaat tgatttaacg caatgttgtc
     2101 atctgttaac tgaattgctt ttgcagaact gtccaacgaa tgaacaagtg atgctccatt
     2161 tttcaacgct gttaaactaa atcctcctga ataacaaaac gtattcaata cttttttgcc
     2221 tttagcgtat tttcctaaaa gcgctctgtt ttcacgttga tcaatgaaaa atccagtttt
     2281 ttgtccgttt atccaatcaa tattaaattt cacaccattt tcaagggcaa catgtggagt
     2341 agggcagtta cccaataaat aaccgtcttt tatttccatt tggtctttgt gtgccaacgt
     2401 atccgacgat ttgttgtaaa tggcaatcac ctcctctttc aataccgaat gaagtgcttg
     2461 tgcaatgtgt tcaaccgctc gatacatccc cacactatgg cattgaataa cggcaacttt
     2521 attgtaataa tctacaatca atcccggcaa accgtcgcct tcaccatgta tcaaacgcat
     2581 gatggaatta tccggacgaa ataaattaag atttcttctt agctgaacgg cattttcaat
     2641 tttcttttga aaaaaattag cgtcaattgc tttattatca aaagataaga tgcgaacggc
     2701 aattgtagca ttttggaaat gaccaacggc taaaaaattg gctttactat ccaatacctc
     2761 aaccacatct ccatcttgca aatcttccga cgccaaatca attgcaccag aaaacaccca
     2821 aggatgtttg cgcatgaccg attctgcttt gtttttgtat aattgaactt gttttttcac
     2881 tttttatatt ttttcacaaa agtaaagaat accatagatt tcacacgcta attatcaact
     2941 taataattta taaatagtgc ttaggtgata aacttcgtag caattctatc aataatttag
     3001 gcgaaatgag ttgaacaaaa actgctacaa cttaattatt ttgtactttt acgccgaaat
     3061 aaaaagtcat ctactaaaaa atgattaaaa acaaactaaa acaatattct caattaacaa
     3121 aattcagatt atcatttaca gtaatggttt ctgcgttagc aggatactta tttaatggtg
     3181 gaacaagctg gagtgaagca atcgttttat cagtcggagg gattttggtt acaggtgcat
     3241 ccaatgcatc caatcaaatt tgggaacgca aactagatgc aaaaatgaag cgcaccatga
     3301 acagaccttt accttcgggc gaaatgtcgg ttacagaagc gtttattgtt gtttttcttt
     3361 ttttaggaat aggtgcctgg atgttgtata gcatcaattg gcaaacagct gcactgggtt
     3421 tatttggtta tttatcttat gtatttgctt atacaccaac aaaaagaatt tctcgctggt
     3481 cggtacttgt aggtgctatc cctggagcgg tgccaccaat gattggagct gttgcagcat
     3541 ctggcgttta ttcatttgtg cctggaattt tatttttcat ccaatttgta tggcaattcc
     3601 ctcatttttg ggccattgct tgggtggcaa acgaagatta caaagcaggg ggattttcgt
     3661 tattaccttc agcatcaggg cgttcaaaac aatcggcgtt gcaaattgca atttatagtt
     3721 tgttgttaat tccgtttagc ttattgcctt ggattttagg gtggacaggc aacgtttcca
     3781 tggttatcat agctatttta ggaggctatt tttattggtt atcctatcgt ttatacatca
     3841 cattggaaat gaaagacgca aaaaaactca tgtttgcatc gtttatttac cttcctttaa
     3901 tacaattcat attggtgttt gatagaaccg aaagttttct tgaacacctc aaaaatgcag
     3961 tcgaaataac atttaaataa aaattcatga aacaacagtt tgaacaagaa ttagatccag
     4021 aaatcagaga aaaactaaga aaaaacttgg tttatgctgc tattggaagt ataatcatgg
     4081 tgtttgctgg attaatttcc gcatacatcg tttcaatggg aggtgcattt tggttgaaat
     4141 ctccacttcc atcagctttt tatgtttcaa cagtgttaat tgtcatcagt agcatcacat
     4201 tttcattggc attaaaagct gcaaaagcaa gaaacttaaa tcaattaaaa atatacattt
     4261 ccatcactct attattgggt attggatttg caatttctca gtttcaaggc tataaacaat
     4321 tggtagaaaa agggttgtat attggttc
//
LOCUS       DAOQVQ010000163         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22737_length_4323_cov_3.702905, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000163 DAOQVQ010000000
VERSION     DAOQVQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22737_length_4323_cov_3.702905"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..2312)
                     /locus_tag="PKN22_06085"
     CDS             complement(369..2312)
                     /locus_tag="PKN22_06085"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42310.1"
                     /translation="MIRKITSIVAFFSLLFTTSMFAQAPIAIDNPIGTIITSNPDGNT
                     PTLICGSSGGDIIGEPNSSGINGVIHRFSNPDWTSYGTSPCLLYGIEYLIPDVIDANS
                     SMPCPSTAMDSFNYDLNLSDLVNGKIVFIGTSAFSYLSGYSFYTSILNTRLTLSITLA
                     GTSTPISVIDDGTRLYFPVLGDFDMRIYIEAESPSSPMGNSTCGGYLAGGVYFGTIEL
                     FDRLSTSSSQQICTSFSSNADSLIHPGYMRFYTNTVTSNATNTGPVAVGLPVSLNGSG
                     SGSGTLAYSWTGPNSYITQDVNISAVAFSDEGVYTLTVTDTFGCSSTTTTTLDVDFDD
                     DGDGLLNSFDNCPYTSNPLQEDTDGDGIGDVCDNCSLISNPLQEDADSDTYGAVCDCD
                     DNNQNVYYSPVIQTVAPLTQTVCYDGSATITIASSEVGLDYELRAIPSGLVVDGPYSG
                     TGSAMSFVANNITSNSSYEVVATMVEYCTTSMSAVSAVVVESPINPITTVDVNTNTIS
                     VNINNADSYQWINCTTENPIVGETGLTYTPIANGTYGVVVTINGCSDTSNCQTINNVG
                     LNTTTYKTVLVYPNPSNGDFYIQSESKIDKVEVLDVVGRVVYSQTNNSNSISVSSLVT
                     GNYLIKLYLDGQVVIKELQIINK"
     gene            complement(2309..>4323)
                     /locus_tag="PKN22_06090"
     CDS             complement(2309..>4323)
                     /locus_tag="PKN22_06090"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42311.1"
                     /translation="VSWNALSLSGSLSSRNYNDVYFFDGQTGIAVGGWETNDSISTII
                     RTTDGGATWSVVMDNIGSMLNSVYFYNANEGYVVGDDGVVYYTNNGGASFSLQTIPAN
                     NTYDNNTVYFVNPYHGLIAGSNGKLLWYNDASAQLAEGTIDSPVTMINSNTVSIIGTV
                     DDKGIIASLEFEYGTTMSFGSTIPFQPNSTDGSGVIPVSVDVSGLTPDLIYFGRMKMT
                     NNVGVSYSNVVSFYTGVSTVPNFNFEIWNEFSNQVLDNWQNMGDIEPATSYNSTTAVR
                     IKGIEGDGIGAILHGSAGQSGFSGGIDFTERPDSIRFYANYNIVPNDSAVVLLILKKN
                     GTPIAFHQWLIGGSTSGNFEYKSYPIEYSTVDYPDTMILIFTSSNPFSGSANENSVMI
                     IDDVTFYGATTNVPNFNMELWTTNIRNKAAFWVSEDDFWNTSSTHIVEKSTDAYSGNF
                     AVKLNNLTQGDADFGGIKAGDSINGWQPAFKVNFNHEKLFGYLKYFPDMNDTLFVRVA
                     MFDDGNQIGYGELKIKDTISSYTLFEVPISYFTSGNADSCLIEFKIFKQNGGNPGNTN
                     AFIDNLSFDMLLAPNLNVNEVLSHNEVTVFPNPTEGPLTIAFENASNELIDILVVDLH
                     GKILLKNQQFLSESKVTIDLSSFRSQQCFVIIKNGNNLYSFKTLIK"
BASE COUNT         1450 a          803 c          680 g         1390 t
ORIGIN      
        1 ttaaaggtta ataaatacaa aaaagtcgga agaaatttct tccgaccatt cgtgtacttg
       61 ttaccgcttt tttttgtttt tggggtttta cacaactaaa aaggtaaagt aaacgctacc
      121 aaaagcagaa aaaacttaat cgctatgggc gcaatggttt tcgcaagggt cgccatgaaa
      181 taatgctcaa acaattaaat tcaacgttgt attctttgcg ccttctttgt gttctttgcg
      241 gtaaattact aaccgttatg gtcgcaatgg tttacgcaga ttaaacacaa aaaaaccgca
      301 ctttaacaat gcggttttcg tttatttatc ctcaattaac gaggggttta aattttaatg
      361 ctgtgggtct atttattaat tatttggagc tcttttatta caacttgacc atccaaataa
      421 agttttatta aataattacc cgtaaccaaa gatgaaacac taatactatt tgaattatta
      481 gtttgtgaat aaacaactct tccaactaca tctaaaactt ctactttgtc tattttactt
      541 tcactttgaa tgtaaaaatc tccattagaa ggatttggat ataccaatac ggttttatat
      601 gttgttgtgt ttaaccctac attgttaatt gtttgacaat ttgaagtgtc agaacatcca
      661 ttaattgtaa ctacgacacc ataagttcca tttgctatag gagtgtatgt caatcccgtt
      721 tcgccaacaa ttggattttc cgttgtacag tttatccatt ggtaagaatc agcgttatta
      781 atattcacag atatggtgtt ggtatttaca tctacagttg tgatagggtt tattggactt
      841 tcaacaacaa ccgctgaaac agctgacatt gaagttgtac aatattctac cattgtagca
      901 acaacttcat aggatgaatt tgaagtgata ttatttgcaa caaaagacat tgcgcttcct
      961 gtaccgctat aaggtccatc aactacaagt cctgatggta tagcacgaag ttcgtaatct
     1021 aaaccaactt cagaggaagc aattgttatt gttgctgaac catcataaca aactgtttga
     1081 gttaatggtg caacagtctg tattactggt gaataataga cattttgatt attatcatcg
     1141 caatcacata cagctccata tgtatctgaa tcagcatctt cttgaagagg atttgaaata
     1201 agtgaacaat tgtcacaaac atctccaatt ccatcaccat cggtgtcttc ttgcagtgga
     1261 ttagaggtgt atggacaatt atcaaaagaa ttcaacaaac catcaccatc atcatcaaaa
     1321 tcaacatcaa gtgttgttgt tgtagtgctg gaacaaccaa atgtatcggt tacagtaagt
     1381 gtataaacac cttcatcaga aaatgcaaca gcggagatgt ttacatcttg tgttatatat
     1441 gaatttgggc ctgtccaact atatgctaat gttccagaac cacttccaga tccatttaac
     1501 gaaactggca aaccaacagc aactgggcct gtattagtcg catttgaagt tactgtatta
     1561 gtataaaaac gcatatatcc aggatgaatt agcgagtctg catttgatga gaaacttgta
     1621 catatttgtt gagaactaga agtacttagc ctatcaaata attcaattgt accaaaatat
     1681 actccacctg ctaaatatcc gccgcaagta gaatttccca tcggagagct tggtgactct
     1741 gcttcgatgt aaatacgcat atcaaaatcg cccaaaacag ggaaatacaa acgtgtacca
     1801 tcatcaatta cagaaattgg tgttgaagtt cctgcaagtg tgatagaaag cgttaaacgt
     1861 gtgtttaata tagaagtgta aaaactatac ccagataaat aggagaaagc cgaagttcca
     1921 ataaatacga tttttccatt tactaaatca gaaaggttta aatcataatt aaaagaatcc
     1981 attgccgttg atggacaagg catagatgaa ttcgcatcaa ttacatctgg aatcaaatat
     2041 tctatcccat acaataaaca cggagaagtt ccatacgatg tccaatctgg attgctaaat
     2101 cgatggataa cgccatttat tccgcttgaa ttaggttcac caattatatc acctccagag
     2161 ctaccacaaa ttaatgttgg agtatttcca tcagggttgg aggttattat agttccaata
     2221 ggattatcaa ttgctatcgg agcttgggca aacatacttg ttgtaaacaa caagctaaaa
     2281 aatgccacta ttgaggttat ttttctaatc attttattaa ggttttaaat gagtacaaat
     2341 tatttccatt ttttattatc acaaaacatt gttgtgatcg gaaagaagat aaatcaattg
     2401 tcacttttga ttcagaaaga aattgctggt tttttaataa aatttttcca tgcagatcaa
     2461 caaccaaaat gtctattaat tcattggaag cattttcaaa tgcaatggtt aaaggacctt
     2521 cagttggatt aggaaaaact gttacttcat tgtgactcaa cacttcatta acattcaagt
     2581 tgggcgctag caacatatca aagcttaaat tatcaataaa ggcattggta ttccctggat
     2641 tgccaccatt ttgtttaaaa attttaaact caattaaaca actatccgca tttccagatg
     2701 tgaaataaga gattggtacc tcaaacaacg tataacttga aatagtatct tttattttca
     2761 attcgccata accaatttga tttccgtcat caaacatagc aacacgcaca aataatgtgt
     2821 cattcatgtc gggaaaatac ttcaaatagc caaacagttt ttcatgatta aaatttactt
     2881 taaaagcagg ttgccatcca ttgatactgt ctccagcttt aattcctcca aagtcagcat
     2941 ctccttgagt taaattattt aatttaacag cgaagttgcc agaataggca tctgtagatt
     3001 tttctactat atgtgtacta gaggtgttcc aaaagtcatc ttctgatacc caaaaagcag
     3061 ctttatttct aatattcgtt gtccataatt ccatattaaa attaggcaca ttagtcgttg
     3121 ctccataaaa agtcacatca tcaataatca ttacgctatt ttcattcgct gatccagaaa
     3181 aaggatttga acttgtgaaa attagaatca tcgtgtcagg ataatctact gtagaatatt
     3241 caattggata gcttttatat tcaaaatttc ctgaggtact tcctccaatt aaccattgat
     3301 gaaacgcgat tggtgttcca ttttttttaa gtataagcaa aacaacagcc gaatcatttg
     3361 gaacgatatt gtaatttgca taaaaacgaa tggagtctgg tctttcagta aaatcaatac
     3421 ctccactgaa gccagattga cctgcagatc catgtaaaat agcacctatt ccgtctcctt
     3481 caattccttt gattcttact gctgttgttg aattataaga tgttgctggc tcaatatctc
     3541 ccatattttg ccaattatct aacacttgat ttgaaaactc attccaaatt tcaaaattaa
     3601 aatttggtac agtcgatact cctgtgtaaa aactaacaac attcgaataa ctcacaccaa
     3661 cattatttgt cattttcatg cgtccaaaat agataagatc aggtgttaac ccagatacat
     3721 ctacagaaac aggaataaca ccagagccat ccgtagaatt tggttgaaaa ggaatggtag
     3781 aaccaaagga catagttgta ccatattcaa attctaaact tgcaattatt cctttgtcgt
     3841 caactgttcc tattattgaa actgtattgg agtttatcat tgttactggc gagtcaatag
     3901 taccttctgc taattgagct gatgcgtcat tgtaccatag caatttacca ttactaccag
     3961 caattaaacc atgatatgga ttaacaaaat aaaccgtatt attgtcatac gtgttgtttg
     4021 ctggaatagt ttgaagagaa aaagaagcgc ctccattatt ggtataataa accacaccat
     4081 catctccaac aacatatcct tcattcgcat tataaaaata tacactattc aacatagagc
     4141 ctatattgtc cattactaca ctccatgtag ctcctccatc cgtcgttcta ataattgttg
     4201 agatactatc gtttgtttcc caaccaccta cagcaatacc tgtttgtcca tcaaaaaagt
     4261 aaacgtcgtt gtaattacgt gaagataaac ttccagataa acttaaagca ttccaagaaa
     4321 cac
//
LOCUS       DAOQVQ010000164         4316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22779_length_4316_cov_4.210749, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000164 DAOQVQ010000000
VERSION     DAOQVQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22779_length_4316_cov_4.210749"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKN22_06095"
     CDS             <1..695
                     /locus_tag="PKN22_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS42312.1"
                     /translation="EIAAFNRSQILYRIAEMLEGRKSQFVEELVFQGLTQKAAQTEVE
                     KSIDRIVYYAGWCDKINQVAGSVNPVSSSHFNFSALQPMGVVALIADPSTSLLGLVSQ
                     ITPIIASGNTVVVLASEALPLCAVTFAEVINSSDVPGGVVNILTGKVDELADVLSSHL
                     DVNAFVSGCNLGQQAVVVAKNCALNVKRFIEMQADWAQENAQGLHYITDCMEVKTTWH
                     PIENIGGSSSNY"
     gene            complement(712..1794)
                     /gene="prfA"
                     /locus_tag="PKN22_06100"
     CDS             complement(712..1794)
                     /gene="prfA"
                     /locus_tag="PKN22_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNS42313.1"
                     /translation="MNDLLQKLEAIHYRFIEVGKMIVDPDIIADMDRYVKLNKEYKDL
                     EEINEVYFEYKNLLGNIQSSKELLEIETDPEMREMAKEEIEISEAKRPEMEEAIKFLL
                     IPKDPEDDKNAIMELRAGTGGDEACIFVEDCLRMYTMYFKEAGFNYEIINTSEGAVKG
                     FKEVSIEISGAGVYGALKFESGVHRVQRVPETESQGRVHTSAITVAVLPEAEEVDFEL
                     NMTDVRKDTFRASGAGGQHVNKTESAIRLTHIPTGVVVECQDGRSQHKNLEKAISVLR
                     SRLYQIELDKIQNERAEQRKTLVSTGDRSAKIRTYNYPQGRITDHRINKTIYNLSAFM
                     NGDIQDMIDALKLAENAEKLKGQEGL"
     gene            complement(1868..2380)
                     /locus_tag="PKN22_06105"
     CDS             complement(1868..2380)
                     /locus_tag="PKN22_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNS42314.1"
                     /translation="MSVIIPNTEFPVFFQHYLNLVDGQLIDELEKQLESYVAFVKSIP
                     KEKELYRYAENKWTVKEVIGHNTDTERVKLDCAFRIARGEQAPLPGFDEDAYVLATNF
                     NDRSMDDLIEEFIYVRKSAIILFKSLRDEDLLKMGTASNKPVSVRALFGFLVGHIRHH
                     ENFLKEHYLK"
     gene            complement(2475..2750)
                     /locus_tag="PKN22_06110"
     CDS             complement(2475..2750)
                     /locus_tag="PKN22_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNS42315.1"
                     /translation="MSKSRFIDSMAADAGISKAAAKKALDSFLTNVGSDLKKGERVSL
                     VGFGSFSVSKRAARSGRNPQTGKTIKIAAKNVVRFKAGSELSGKVNK"
     gene            complement(2984..3778)
                     /locus_tag="PKN22_06115"
     CDS             complement(2984..3778)
                     /locus_tag="PKN22_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS42316.1"
                     /translation="MLRYLFILVFVGALLAHSRSSHFFGISEADLMDGIKLETVMLND
                     NCRERLVPIAIYTSYADSAKQNLPVIIFNHGYGKNQGDSYLTYSYINEFLASKGYCVI
                     SIQHELKTDEKLPYQGNLQELRKPFWDRGAENINYVIKELDSIRPQLNFKKVVLIGHS
                     NGGDIIANYALQHPDSIKSIITLDNLRMPLPKNGSFPVLSIRASDTEPDPGVIPTLAD
                     QKKYGMKIIYLPNTNHGDMNDKATDEQKEEINQELAYFLKSKKKTS"
     gene            complement(3843..>4316)
                     /locus_tag="PKN22_06120"
     CDS             complement(3843..>4316)
                     /locus_tag="PKN22_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNS42317.1"
                     /translation="TSIQTFTGAGKRLQKVKETADFVLFKDFAHSPSKLKATTKAVKE
                     QYANRTVVACMELHTFSSLKKEFLPHYKDAMKAADVAIVYFNHEVVAHKKLEPITEQQ
                     VFDGFNGGITVMTQTADVLQFIKSQDWHNKVLLMMSSGNFDGIDYEQLGNEITKD"
BASE COUNT         1269 a          795 c          773 g         1479 t
ORIGIN      
        1 cggaaatagc ggcttttaac cgcagtcaaa ttctttaccg cattgccgaa atgttggaag
       61 gaagaaaaag ccaatttgtg gaagagcttg tttttcaggg attaacgcaa aaagcagcac
      121 aaacagaagt tgaaaaaagc attgatagaa ttgtatatta tgctggttgg tgcgataaaa
      181 tcaaccaagt ggcaggaagt gttaatcctg tttcgtcttc gcattttaat ttttcggctt
      241 tgcaaccaat gggtgtggtg gcattaattg ccgatccatc tacttctttg ttagggttgg
      301 tttcgcaaat tacaccgatt attgctagtg gaaacacggt tgtagtactt gcttcagaag
      361 cattacctct ttgtgcggtt acgtttgcag aggtcatcaa ttcgtcggat gtacctggtg
      421 gtgttgtgaa tattcttaca ggaaaagtag atgaattggc cgatgtgtta tcttctcatt
      481 tggatgtaaa tgcctttgtg agtggctgca atttaggtca acaagcagtt gtggtagcaa
      541 aaaattgtgc tttgaacgta aaacgcttta tagaaatgca agccgattgg gcgcaagaaa
      601 acgctcaagg attgcactac ataaccgatt gtatggaagt aaaaactact tggcacccga
      661 tagaaaacat cggaggaagt agttctaatt attaaagatt ttagggaaga tttacaatcc
      721 ttcttgacct tttaattttt cagcattttc ggctaatttc aaggcgtcaa tcatgtcttg
      781 aatatcacca ttcataaacg cagataaatt gtaaatggtt ttgttgatac ggtgatcggt
      841 aatacgtcct tgcggataat tataggttct gattttagca gaacggtcac ctgtagaaac
      901 taaggtcttg cgctgctcgg cacgttcatt ttgaatttta tccaactcaa tttgatacaa
      961 tcgtgaacgc aatacagaaa ttgctttttc taagtttttg tgttgagaac gtccatcctg
     1021 acattcaacc accacacctg ttggaatgtg tgttaaacga atagccgatt cggttttgtt
     1081 gacgtgctga cctccagctc cagatgctct aaacgtatct ttgcgcacat cggtcatgtt
     1141 gagttcaaaa tccacttctt cggcttctgg caatacagcc accgtgattg cagaagtatg
     1201 tacacgtcct tgcgattctg tttcaggtac acgttgaaca cgatgcacac ccgattcaaa
     1261 ttttaaggca ccatatactc cagcacccga aatttcaatc gaaacctctt tgaatccttt
     1321 tacagcacct tccgaagtat tgataatttc atagttaaaa cctgcttctt tgaagtacat
     1381 ggtgtacata cgcaagcaat cttcaacgaa aatacaagct tcatcaccac cagttccagc
     1441 acgcaactcc ataatggcat ttttatcgtc ttctggatct tttggtatca acaaaaattt
     1501 gatggcttct tccatttctg ggcgtttggc ttctgaaatt tcaatttctt ctttcgccat
     1561 ttcacgcatt tctgggtcgg tttcaatttc taaaagttct ttcgaagatt ggatgttgcc
     1621 caaaaggttt ttatattcaa aatacacctc gttaatttct tcaaggtctt tgtattcttt
     1681 gtttaatttg acataacgat ccatatccgc aatgatgtcg gggtcaacaa tcatttttcc
     1741 aacttcaata aatcggtaat ggatagcctc tagtttttgt aataaatcgt tcatgtctct
     1801 aatttgggtt gcaaatatag agattttagt ttgaaaaata gttgtttaat tctgtatggt
     1861 ttgacaatta ttttaaataa tgttctttca aaaagttttc gtgatggcga atatgaccta
     1921 ccaaaaaacc aaataatgca cgcacactca ccggtttgtt tgatgctgtt cccattttta
     1981 acaaatcttc atcgcgcaac gatttgaaaa ggatgatggc actttttcta acgtaaataa
     2041 attcctctat taaatcgtcc attgatcggt cgttaaaatt ggttgccaac acatacgcat
     2101 cttcatcaaa tccaggaaga ggtgcttgtt ctccacgtgc aatcctaaaa gcacaatcca
     2161 gttttacacg ctccgtatcg gtattgtgtc caatcacttc tttcaccgtc catttattct
     2221 ccgcatagcg gtatagttct ttttcttttg gaatagattt aacaaacgct acgtaggatt
     2281 ccaattgttt ttccaattca tcaattaatt gaccatcaac caagtttaaa taatgttgaa
     2341 aaaatacggg gaattctgta ttggggataa tgacactcat gattctattg tttgttttca
     2401 aatttagtta taaattatta agtaacaaaa aaggctaccc aaattggata gcctttacat
     2461 taagaagtaa aatcttattt gtttactttt cctgaaagtt cagatcctgc tttgaaacga
     2521 acaacgtttt tagcagcgat tttgatagtc ttaccagttt gtgggttacg acctgatcta
     2581 gcagctcttt tagaaactga aaaagatccg aaaccaacta aagaaactct ttctcctttt
     2641 ttcaaatctg aaccaacgtt agttaagaaa gaatctaacg ctttttttgc tgctgcttta
     2701 gaaattcctg catctgcagc cattgaatca ataaatctag acttactcat agataatagt
     2761 ttttatgtta ttaattattt taactttaac aaatatattc ataatccctt acggtgcacc
     2821 gttttattaa aaaaaaatgc aaaaatgttg ataaatctgc ctttttgttg ataaactagg
     2881 ggtaaaagtg cctatttaca aggggtttca ctaattttag ggcttaaatt agcttgggaa
     2941 aatcaagtgt tagaatataa gaaatgccta aaattgaaaa aaactaactt gttttctttt
     3001 tagactttaa gaaataagct aattcttggt taatttcttc tttttgctca tccgttgctt
     3061 tatcattcat atcaccgtgg ttggtgttgg gcaaataaat gattttcatg ccgtattttt
     3121 tttgatcggc caaagttgga attactccag gatcgggttc ggtgtcactg gcacgtatag
     3181 acaatacagg aaatgaacca tttttaggca atggcattct taaattatcc aatgtgatga
     3241 tgcttttgat agaatctgga tgttgcaacg cataattagc aataatgtcg ccaccgttgg
     3301 agtgaccaat caatacaact ttcttgaaat taagttgcgg acgaatggaa tccaattctt
     3361 ttattacata attaatgttt tcggctcctc tatcccaaaa tggcttgcgc agttcttgta
     3421 aattcccttg gtagggtaat ttctcgtcag tttttaattc gtgctgtatg ctaatcacac
     3481 aataaccctt tgaagccaaa aattcgttga tgtatgaata ggttaaataa ctatcgcctt
     3541 ggtttttgcc ataaccatga ttgaatatta taacaggtaa attttgtttt gcactatcgg
     3601 cgtaactggt gtaaatggca attgggacta ggcgttctct gcaattgtcg ttcaacatca
     3661 ctgtttccaa cttaatgccg tccattaaat cagcctcgct aataccaaaa aaatggctag
     3721 agcgcgaatg agctaacaag gcaccaacaa aaactagtat aaataaatag cgtaacaaat
     3781 tctatctcaa tttatttgtt gcaaatttaa ttcaaataag aatactaagt tataataaat
     3841 tgttaatctt ttgtaatctc attgcctaat tgttcataat ctatgccgtc aaaatttcca
     3901 gatgacatca ttaaaagtac tttattatgc caatcttgac ttttgatgaa ttgaagaaca
     3961 tccgctgttt gtgtcattac cgttatgcca ccattgaaac catcaaacac ttgttgctcg
     4021 gtaattggtt ctaatttttt gtgagcaaca acttcatgat taaaatacac aatggccaca
     4081 tctgctgctt tcatcgcatc tttgtagtgg ggtaaaaatt cttttttcaa ggaggagaat
     4141 gtgtgcaatt ccatacatgc taccactgtt ctgttagcat attgttcttt tacagctttt
     4201 gttgttgcct ttaattttga aggagaatgt gcaaagtctt tgaatagcac aaaatcggct
     4261 gtttctttca ctttttgcag gcgttttcct gctcctgtaa atgtttgtat gctggt
//
LOCUS       DAOQVQ010000165         4298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22913_length_4298_cov_4.344096, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000165 DAOQVQ010000000
VERSION     DAOQVQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22913_length_4298_cov_4.344096"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKN22_06125"
     CDS             <1..420
                     /locus_tag="PKN22_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42318.1"
                     /translation="YDDYPKEELNRYNAKGNFYYDKQSKHIISDYYPAEGLTPRISAF
                     ADVMDTVRLNKVLYEKNRLEKVTISINQRTIPGDFYDWLIKQPYRKEFELLDEQLEIR
                     IFDVAQDDIEVIQKMASLFGFNFSDKRNEIENEQNNE"
     gene            413..685
                     /locus_tag="PKN22_06130"
     CDS             413..685
                     /locus_tag="PKN22_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNS42319.1"
                     /translation="MNNTQVQEEAAVLEKIVEQYDLIVYNDDVNTFDFVIESLVKVCD
                     HNPHQAEQCTMIIHYNGKCQVKRGKYEQLELMCTALLDRGISAVIE"
     gene            758..831
                     /locus_tag="PKN22_06135"
     tRNA            758..831
                     /locus_tag="PKN22_06135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:792..794,aa:Arg,seq:acg)
     gene            906..1715
                     /locus_tag="PKN22_06140"
     CDS             906..1715
                     /locus_tag="PKN22_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS42320.1"
                     /translation="MKKTTYALVTGASSGIGLKIANTLALKKYNLLLVSNQNEELQKV
                     GFEISQTHQVECKTLCLDLANTAAAQEIFNYCEQQQISIEVLVNNAGMLVFSEVTATD
                     KTKVNTILQLHMCTPTLLCRLFGEEMKKMKKGYILNVSSISSVMPYPGISLYGPTKTF
                     MRYFTRAFRSEMKIYNVHVTCLLPGATETGLYDPNKVNLKLAKRTGIMQTSEFVSNKA
                     INALFKNKAECIPGIINKLTVWFLPLLPSWLIFQIHKNTNLIQKGNQALDK"
     gene            complement(1743..2357)
                     /locus_tag="PKN22_06145"
     CDS             complement(1743..2357)
                     /locus_tag="PKN22_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNS42321.1"
                     /translation="MSDLLHALEERFGAHRVVAIPTAPNELPLIAIDIESRSQVTVIT
                     TNGLADYKMPIPEKLAGREYNELCFCLPSYWEWENVDNPSMNWIFPWIQKMAQYVVEK
                     NTWFGPGHTIPNGKEALPLSPTMQQNYFLISDPILLEQELAPITVGEKNIHFLTIIPI
                     FEDEMDMKAEKGSAKFFKKFMEKGGTEILDDYRRSTLKSKWRMF"
     gene            complement(2365..2838)
                     /locus_tag="PKN22_06150"
     CDS             complement(2365..2838)
                     /locus_tag="PKN22_06150"
                     /inference="COORDINATES: protein motif:HMM:NF039798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6495 family protein"
                     /protein_id="HNS42322.1"
                     /translation="MKYRLLTDDELSHFEGDLKAFLIVNGIDGPEWEKINKETPEKAL
                     GLIEIFSDNVLEIVYSKIHYAEFRAPKSLMVFKINENSTDLISLQLPENSTANFSTVE
                     SIHAALTQHAGEIQFFRQSRKHTKTREFEVHALIDQGCVPSDEEFWLSLDTITSN"
     gene            complement(2848..4038)
                     /locus_tag="PKN22_06155"
     CDS             complement(2848..4038)
                     /locus_tag="PKN22_06155"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNS42323.1"
                     /translation="MEQIIGDLLIRHNCVIIPEFGGFVTQRTAAKIDTNLGVITPPSK
                     SLLFNRQLINNDGLLIAELAQQTRVSYDEARLMVQQLVKSWNETLSSGQRITIDKVGF
                     LFFDQERNICFEQDRYFNLLLSSYGLGNVHFLTETDVAIAQHKAIQQEKLEVAAPIEA
                     VATSIVQPVHGQPQPIVEQQPTVEPVVEPAQPTIQVVRKKSNVWKYAAAACLIPVLFY
                     SFWIPMKTNVLESKIISLQDFNPFHKHKAGEYQPQKTKIQINQIDEPTLEEQMNEIES
                     NESTYSLKFSDDTYVLVDTNKPAQEENAVATENKTVVSNNLNTTTAVSSASMHVIVGC
                     FSDATNAQTLVKELQQKGFQASIKDVVNGLSRVSIGGTNTQEELNQLMAKAKSAGFNG
                     WVLK"
     gene            complement(4097..>4298)
                     /locus_tag="PKN22_06160"
     CDS             complement(4097..>4298)
                     /locus_tag="PKN22_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNS42324.1"
                     /translation="HDYEITDGKGTAIGKVTSGTMSPSLKLGIGLGYVPTANAALDSE
                     IFIKVRDKELKAKVVKAPFYKK"
BASE COUNT         1377 a          794 c          751 g         1376 t
ORIGIN      
        1 tacgatgatt atccaaaaga agaattgaac cgttacaatg caaaaggaaa tttctattac
       61 gataagcaga gtaaacacat aatttctgac tattatccgg ctgaaggttt aactcctcga
      121 ataagtgcat ttgccgatgt gatggataca gttcgtttaa acaaagttct ttacgaaaaa
      181 aatcgcttag aaaaagtaac tatttcaatc aatcaacgta cgatacctgg agatttttat
      241 gattggttga tcaaacagcc ctacagaaaa gaatttgaat tattggatga gcaattggaa
      301 attcgtatct ttgatgtagc gcaagatgac attgaagtaa ttcaaaaaat ggcttcgcta
      361 tttggtttca acttttcgga taaaagaaat gaaattgaaa atgaacaaaa caatgaataa
      421 cacacaggtt caagaagaag cagcagttct tgagaagatt gttgagcaat acgatttaat
      481 tgtttacaac gacgatgtca acacgtttga ttttgtgata gaatcgttgg ttaaagtttg
      541 cgatcacaat ccccatcaag ccgaacaatg caccatgatt atccactaca atggtaagtg
      601 ccaagtgaaa cgtggaaaat atgagcaatt agaattaatg tgtacagcat tgttagacag
      661 aggaataagc gctgtaattg aataatttta aaaataaatc aaaaaaaaca ttgcattttt
      721 agaaaaatac actaattttg cactcgctaa taagcacggc tccgtagctc aactgtatag
      781 agcatcagat tacggctctg aaggtttcag gttagaatcc tgacggggtc acaaaaaagt
      841 cagtcagttt tgattgactt tttttatatc cttttatttc agcaatttta tactattttt
      901 gaattatgaa aaaaactact tatgcactcg ttactggtgc ttcttctgga attggactta
      961 aaattgcaaa cacacttgct ttaaaaaagt acaatttgct tcttgttagc aatcaaaatg
     1021 aagaattgca aaaagtgggg tttgaaattt cgcaaacgca ccaagtggaa tgtaaaacac
     1081 tttgtttaga cttagcaaat acagctgctg cgcaagaaat ttttaactac tgtgagcaac
     1141 aacaaatatc tattgaagta ttggtgaaca acgctgggat gttggtattt tcagaagtta
     1201 ctgctaccga caaaacaaag gtgaacacaa ttctccaatt acacatgtgt acgcctaccc
     1261 tactttgtcg attgtttggt gaagaaatga aaaaaatgaa aaaaggatat atcttaaatg
     1321 tctcatccat ttcttcggta atgccctatc caggaatttc actttatgga ccaacaaaaa
     1381 cctttatgcg ttattttaca agggctttta gaagtgaaat gaaaatttac aacgtgcatg
     1441 ttacttgtct tctgccaggc gcaaccgaaa caggtttgta tgacccaaat aaagtaaatt
     1501 taaagttagc taagcgaact ggcattatgc aaacttccga atttgtttcc aataaagcaa
     1561 tcaacgcatt gtttaaaaac aaagcagagt gcattcctgg aattatcaat aaacttacgg
     1621 tttggttttt acctttattg cctagctggt taatatttca aatccataaa aacaccaatt
     1681 taatccaaaa aggaaatcaa gcgttggata aataaatgga cggaatttaa aatgtgttgt
     1741 tactaaaaca ttctccactt actttttaat gtcgatctgc ggtaatcgtc caaaatttcg
     1801 gtaccgcctt tttccataaa ttttttaaag aatttagcgc ttcccttttc tgctttcatg
     1861 tccatttcgt cttcaaaaat tggaatgatg gtcagaaaat gaatgttttt ttctccaacg
     1921 gtaattggtg ctaattcttg ctccaataaa attgggtcgg aaatgaggaa ataattttgt
     1981 tgcatggtgg gcgataatgg caatgcttct ttaccgtttg gaatggtgtg tccgggacca
     2041 aaccacgtat ttttctccac aacatattga gccatttttt gaatccaagg aaaaatccaa
     2101 ttcatcgatg ggttatccac attttcccat tcccaataac ttggcaaaca aaaacacaat
     2161 tcgttgtatt cgcgtccagc caacttttct ggaataggca ttttataatc cgccaaacca
     2221 ttggttgtaa ttaccgttac ttgcgagcgg gactcaatat cgatggcaat caaaggcaat
     2281 tcgttgggag ctgtaggaat ggcaacaact cgatgtgcgc caaagcgttc ttccaatgca
     2341 tgtaataaat ccgacatttt ttatttaatt ggacgttata gtgtccaaag ataaccaaaa
     2401 ctcttcatct gagggtacac aaccttggtc aattaatgca tgtacttcaa attcgcgtgt
     2461 tttggtgtgc tttcttgact gacgaaaaaa ctgaatttca cctgcatgtt gtgtcaatgc
     2521 cgcatgaatg ctttcaaccg ttgaaaaatt ggcggtagaa ttttcaggca attgcaacga
     2581 aattaagtcg gttgaatttt cattgatttt aaacaccatc aacgattttg gtgctctaaa
     2641 ttcagcataa tgaattttac tgtaaactat ttccaacaca ttgtcagaaa atatctcaat
     2701 caagcccaat gctttttctg gagtttcttt gttgattttt tcccactccg gaccatcaat
     2761 tccgttgaca attaaaaatg cttttaaatc accttcaaaa tgtgaaagtt catcatccgt
     2821 taacaaacga tatttcataa aggaaaatta tttgagaacc caaccgttaa agcccgccga
     2881 tttggctttt gccatcaatt ggttcaattc ttcttgtgta ttcgttcctc caatcgaaac
     2941 gcgtgataga ccatttacaa catctttaat agaggcttga aaaccttttt gttgcaattc
     3001 ttttaccaac gtttgggcat ttgtagcatc cgaaaaacaa cctacaatta catgcatgga
     3061 tgctgatgaa acagcagttg ttgtgttcaa attattggaa acaacagtct tattttctgt
     3121 tgcaaccgca ttttcttctt gagcaggttt gtttgtatcc accaacacat aagtatcgtc
     3181 tgaaaattta agtgaatagg ttgattcgtt ggattcaatc tcgttcattt gttcctccag
     3241 cgttggctca tcaatttgat ttatctgaat tttagttttt tgaggttgat attctcctgc
     3301 tttgtgttta tgaaatggat taaaatcttg caacgaaata atcttcgact ccaacacatt
     3361 ggttttcatc ggaatccaaa aagaataaaa taaaacaggt atcaaacacg ctgcggctgc
     3421 atatttccaa acattggatt ttttgcgaac aacctgaatt gttggttgcg caggttcaac
     3481 tactggttca actgttggtt gttgctccac tattggttgc ggttgaccat gaacaggttg
     3541 aacaatagaa gttgctaccg cctcaattgg agcagcaact tctaattttt cttgttgaat
     3601 agctttatgt tgtgctattg ccacgtcggt ttcagtcaag aaatgaacgt tcccaagtcc
     3661 atacgacgat aatagcaaat taaaatagcg atcttgttca aagcaaatat tgcgctcttg
     3721 atcaaaaaat aaaaagccaa ctttatcaat ggtgatgcgt tgccccgaac tcaatgtttc
     3781 gttccacgat tttaccaatt gttgcaccat caaacgggct tcatcataag aaacacgtgt
     3841 ttgttgcgcc aattcggcaa tcagcaaacc atcgttgttg attaactggc gattgaacaa
     3901 aagcgatttg gaaggcggtg taataacccc taaattggta tcaatttttg cagcagtacg
     3961 ttgcgtaaca aacccaccaa attcgggaat gatcacgcaa ttatggcgaa tcaataaatc
     4021 tcctataatt tgttccatct gcatcattta cacaaaaata aaaaaacctt ccaataaatt
     4081 caattaaacc tgttgcttat tttttataaa atggtgcttt tactacttta gctttcaatt
     4141 ctttatctct tactttgata aaaatttcag aatctaatgc agcatttgct gttggcacat
     4201 agcccaatcc aatacccaat ttaaggcttg gcgacattgt tccagaagtt actttaccaa
     4261 ttgcagttcc tttgccatca gttatttcat aatcatgg
//
LOCUS       DAOQVQ010000166         4292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_22962_length_4292_cov_3.531744, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000166 DAOQVQ010000000
VERSION     DAOQVQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22962_length_4292_cov_3.531744"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PKN22_06165"
     CDS             complement(<1..1650)
                     /locus_tag="PKN22_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42325.1"
                     /translation="MKQLLFFLFLIFVGTTTAQTEALSPVERAYLFHIVKKSPILNDN
                     IGRYFDYTGEEIKFANGNINYDSLESVIINQPELLVIHTAEIAKSTKGILAEAANKTA
                     IWELNKMLLAKRTKDALFETYKPRYAEFEAILLQYMPSSVLNDEQNGLHGRMDNVLNP
                     SLSLDDKVAQLSSFRKMSNDDALLVIKGISKAINEYVEKRTKEIFVYLGGDYTTFRNV
                     LTAVGDGSITSGLLEEREKDEKGRWNKGLPRAIGLFPYEVYLTKPTSKKEQAKIEPYR
                     FTTTDLKTVGENRATNLHFDVWGYNAKKQTTVVIEKNGKTYHLFGSNETRFLSPDSTF
                     SSGETFQSIINKLEFDMIAKITEKIEGKRGYDYWIETYTQKKRETELKIVKSEKSYSD
                     LGYSPITTSSKPSAKVKKAKKRAIKAGVGADNWDAAPTTDAGKKQKGKGQQTIVDLYN
                     QFDWYKAKIKELEKEKQEAIDLRARYQLRLSEYKQQMGYNWVPFTVNNGLYTFADSAT
                     FDLYTQEFQFPPSDKVEDFEVRLIAIPDSPLSSNADEVMLHL"
     gene            complement(1692..2099)
                     /locus_tag="PKN22_06170"
     CDS             complement(1692..2099)
                     /locus_tag="PKN22_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42326.1"
                     /translation="MKTKHLLLVLVAFILAACSKEITTPDEQSKLIFGKWSLNHISGG
                     YSGAGEEVTDVREVEFLSNGKSKWYVNSNIDHKAKFTLKKVNNMFGENQLEIDYAGDG
                     KINHTVFELSDHDLVLSENAYDGYTYHYKRMSN"
     gene            complement(2138..3148)
                     /gene="murB"
                     /locus_tag="PKN22_06175"
     CDS             complement(2138..3148)
                     /gene="murB"
                     /locus_tag="PKN22_06175"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNS42327.1"
                     /translation="MIQTNVDISSFTTFGLPARVKQFATFSSVDELKQLLQQKPENTP
                     LMLLGGGSNVLFTQDFDGLLLKNEIMGFEIVEKSGDDVLVKAGAGENWHSFVIRTINL
                     NLGGLENLSLIPGSVGASPMQNIGAYGREIKDVFHSLEAYHLPTGEIHTFNNQQCGFG
                     YRESVFKRQFKNQYVIVSVTYQLTKKHQLNTSYGAINEQLQAMGIENPTIKDVSNAVI
                     AIRSSKLPNPKEIGNAGSFFKNPVIEKQLYNQLVANYPDMPNYPVDELHVKVPAGWLI
                     EKAGWKGKTFDNYGVHKNQALVLVNYGGATGKQVFELSEAIIADIQSKFNIALEREVN
                     II"
     gene            complement(3259..3705)
                     /locus_tag="PKN22_06180"
     CDS             complement(3259..3705)
                     /locus_tag="PKN22_06180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS42328.1"
                     /translation="MKKLIYFLLLALLVTSCSTYSDEELQAFDKKIATYVKKHNLKMT
                     KSDSGMYYQIHEKGSGDSIKANAMISTIYTGRLLNGKVFDQTKEPVELQLNQLIMAWR
                     ELAFYIREGGKATIIVPPQVGYGSRSMPDIPKNSILIFDIEVLATY"
     gene            complement(3723..>4292)
                     /locus_tag="PKN22_06185"
     CDS             complement(3723..>4292)
                     /locus_tag="PKN22_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNS42329.1"
                     /translation="PEQQDSLLQWAHTQLQVDSAQFMLSYLKMKPFVLVQLATQKELV
                     DKTKSYDMTIQQMANEQKISTVGLETVAEQMAIFDNMDTLTQRNMVMEFIRHPEGQLE
                     QLEKMYAIYLQQNVDAMFQFIDEDKSSMMDAQNELLDERNQKWIPKIETLIKSKRTFI
                     AVGAGHLGGKNGVLRLLEKKGYKLTPIHL"
BASE COUNT         1240 a          840 c          708 g         1504 t
ORIGIN      
        1 taagtgcaac ataacctcat ctgcattgga cgacaatggt gaatcgggaa tagcaatcaa
       61 ccgcacttca aaatcttcta ctttatcaga aggtggaaat tgaaattctt gggtgtataa
      121 atcaaacgtt gcagaatctg caaaggtgta caagccattg ttgactgtaa acggaaccca
      181 attataaccc atttgttgtt tgtattcact caaacgtaat tggtagcgtg cacgtaaatc
      241 tatagcttct tgtttttctt tttccaattc tttgattttg gctttatacc aatcaaattg
      301 gttgtacaaa tccacaatag tttgctgccc tttgcctttt tgtttttttc cggcatcggt
      361 tgtaggtgct gcatcccaat tatcggctcc aacacctgct ttaatagcgc gtttttttgc
      421 tttttttact tttgctgatg gtttgctgga agtggtaatg ggactgtagc ccaaatctga
      481 gtagcttttc tctgatttta caatttttaa ttctgtttct ctttttttct gagtataagt
      541 ttcaatccaa taatcataac ctcgtttccc ttcaattttt tcggtaattt tggcaatcat
      601 atcaaactcc aacttattga tgatgctttg aaatgtctct ccggatgaaa aagtactatc
      661 tggcgaaaga aaacgtgttt cgtttgaacc aaacaaatgg taggtttttc catttttttc
      721 tatcaccacc gttgtttgtt ttttagcatt gtatccccac acatcaaaat gcaaattggt
      781 tgctcggttc tctcccactg tttttaaatc ggttgtagtg aaacgatagg gttcaatttt
      841 agcttgttct tttttagaag ttggtttggt tagatacact tcatacggaa acaagccaat
      901 ggcacgtggc aatcctttat tccaccttcc tttttcgtct ttttcacgtt cttctaacaa
      961 cccagaagtg atgctaccat caccaactgc tgttaaaaca tttctaaaag tggtataatc
     1021 gccacccaaa tacacaaaaa tttctttggt gcgtttttcc acatattcat taatcgcctt
     1081 ggaaatacct ttgatgacta acaacgcatc atcgtttgac attttacgga acgagctcaa
     1141 ctgtgccact ttatcgtcaa gcgacaaact tgggtttaac acattgtcca tgcgcccatg
     1201 caacccattt tgttcatcgt tgagcaccga agatggcata tattgtagca aaatcgcctc
     1261 aaattcagca tatctaggtt tataagtttc aaaaagagca tccttggtgc gttttgccaa
     1321 caacatttta ttgagttccc aaatggccgt tttattagct gcttcggcta aaataccttt
     1381 ggtagatttt gcaatttctg ctgtatgtat caccaacaat tctggttgat tgatgataac
     1441 gctttccaat gaatcgtaat taatatttcc attcgcaaat tttatttctt ctcctgtgta
     1501 gtcaaaatag cgaccaatgt tatcgtttaa aatcggactt tttttaacga tgtgaaacaa
     1561 ataggcacgt tcaactggcg acaaagcttc tgtttgtgca gtggttgttc caacaaaaat
     1621 caagaaaagg aaaaataaaa gttgcttcat atttttttat taaataactc agaaaatgaa
     1681 ttttgtaaca cttaattact cattctttta taatggtatg tatagccatc ataagcattt
     1741 tcacttagga ctaaatcatg atctgacagt tcaaaaacgg tgtgattgat ttttccatct
     1801 ccagcataat caatttcaag ttggttttca ccaaacatgt tgttgacttt tttaagtgta
     1861 aattttgctt tatggtctat attgctatta acataccatt ttgatttccc attggaaaga
     1921 aattcaactt ctctcacatc tgttacttcc tcacctgcac cactatatcc accagaaatg
     1981 tgatttaagc tccatttgcc aaaaattaat tttgattgct cgtctggtgt agtaatttct
     2041 ttggaacatg cagcaagaat gaaagctaca agtactaata aaaggtgttt tgttttcata
     2101 ttatttagtt gaattgattt ttaacttatt aaaactacta aataatattc acttcacgtt
     2161 ccaacgcaat gttaaatttg gattgaatgt cggcaataat ggcttctgaa agttcaaaca
     2221 cttgtttacc cgttgcgcca ccataattaa ccaataccaa cgcttgattt ttgtgcacac
     2281 catagttatc aaatgttttg cctttccaac ctgctttttc aatgagccaa cctgcaggta
     2341 cttttacatg cagttcatcc acaggatagt tgggcatatc gggatagttt gccaccaatt
     2401 gattgtacaa ttgtttttct atcaccggat ttttaaaaaa actacccgcg ttgccaattt
     2461 cttttggatt gggtaatttg ctactgcgaa tggcaataac ggcattactc acatctttga
     2521 tggttggatt ttcaattccc attgcttgca attgctcatt aatggcgcca taactggtat
     2581 tgagttggtg ttttttagtc aattgataag ttacactcac aattacgtat tgatttttga
     2641 actggcgttt aaacacactt tcgcgatagc caaaaccaca ttgttggttg ttgaacgtat
     2701 gaatttctcc cgttggaaga tggtaggctt ctaatgaatg aaaaacatct tttatttctc
     2761 tgccataggc tccaatattt tgcatagggc tcgcacccac acttcctgga atcaacgata
     2821 agttttccaa tccgcccaag tttaagttga tggtgcgaat tacaaaagaa tgccaatttt
     2881 cacctgctcc tgctttcacc aacacatcat cgcctgattt ttcaacgatt tcaaaaccca
     2941 tgatttcatt tttgagcaaa agtccatcaa aatcttgtgt aaaaagcaca ttgctaccac
     3001 cacccaacaa cattagcggg gtgttttctg gcttttgttg caacaattgc ttcaactcat
     3061 caaccgaaga aaaagttgca aattgtttta cacgtgcggg caatccaaaa gtggtaaaac
     3121 tcgaaatatc aacgtttgtt tgtatcataa gttcaaaaat aatgaaactc ccatcatcat
     3181 ttttaacttc ttacaacagt ttccaacaat aaaaagttat aaaacaagaa agtctagctt
     3241 tttaaaaagc tgagaaaatt aataagtggc taaaacctca atatcaaaaa tcaaaatgga
     3301 atttttcgga atatcaggca tagagcgaga accgtagcct acttgtggtg gcacaatgat
     3361 agttgctttt ccaccctcgc gaatataaaa agctaactcg cgccaagcca taattagttg
     3421 atttaattgc aattctacag gttctttggt ttggtcaaat acttttccat tcaataaacg
     3481 tccggtgtaa atggtgctaa tcattgcgtt tgctttgata gaatcaccac tacctttttc
     3541 atgaatttga taatacattc ctgaatctga cttggtcatt ttcaagttgt gttttttcac
     3601 atatgtggca attttcttat caaatgcctg taattcttca tcgctgtacg ttgaacaaga
     3661 tgttaccaat aaggctagta ataggaaata aatgagtttt ttcatgcttc aaatataaaa
     3721 acttatagat gaattggtgt taatttataa cctttctttt ccaataaacg caaaacgcca
     3781 tttttaccac ccaaatggcc agcacccaca gcaataaatg tgcgtttgct ttttatgagt
     3841 gtttcaattt ttggaatcca cttttgattg cgttcgtcca ataattcatt ttgagcgtcc
     3901 atcatgctgc ttttgtcttc atcaataaat tgaaacatag catccacatt ttgttgaagg
     3961 taaatggcat acattttttc gagttgttct aattgtcctt cgggatgacg aataaattcc
     4021 atcaccatat tgcgttgtgt cagggtgtcc atgttatcaa aaattgccat ttgctcagct
     4081 acagtttcaa gtccaacagt tgatattttt tgctcattgg ccatttgttg gatggtcata
     4141 tcgtaggatt ttgttttatc taccaactct ttttgggttg ccaactgcac caaaacaaac
     4201 ggtttcattt taaggtaaga aagcatgaat tgagcagaat caacctgtaa ttgtgtatga
     4261 gcccattgca acaaagaatc ttgctgttcg gg
//
LOCUS       DAOQVQ010000167         4258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23208_length_4258_cov_3.165834, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000167 DAOQVQ010000000
VERSION     DAOQVQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23208_length_4258_cov_3.165834"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PKN22_06190"
     CDS             complement(<1..1362)
                     /locus_tag="PKN22_06190"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNS42330.1"
                     /translation="MNEINHAKGLLKLIFGALFIPSITLITTTSTLHAQCPPTANAGN
                     DITIPCGGSANLGGPASNPTPDYTLAPTCMHTARFQSNSLTQGGGIYINTVTTTGGTT
                     NINNPNTLVDVGHIDPMGPNAWSTIWLSDFTSQYVEVCVGNTFYLNITAKSLYNNQPY
                     FCKVWVDWNNNGTFENASEVVYTSPAINNHPNIILTNIPITVPAGQPYGAYRMRIRFK
                     DNAPFVASDGGCTYTNPVGIEAPYAGYTSGTGSYTFSDEIEDYTVRVNCGGSSGGTLT
                     YSWSPPDGLSSTTVPDPTATPTQTTTYTVTVTDTQHGCSGTAQVTVTVPSVTPTFNPI
                     SPICNGDVAPALPTTSTNGITGTWSPATISNTASGTYTFTPDGGQCATSATISVTVNP
                     NPTITVTNGEYCENGSATLTANGATTYSWSPGTNLSATTGATVTTTATASTTYTVTGT
                     DNNG"
     gene            1585..2952
                     /gene="hisS"
                     /locus_tag="PKN22_06195"
     CDS             1585..2952
                     /gene="hisS"
                     /locus_tag="PKN22_06195"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNS42331.1"
                     /translation="MAQKPSIPKGTRDFLPIEVARRNYLFNTIKEVYKKYGFAPIETP
                     SFELSSTLLGKYGEEGDRLIFRILNSGEKMKKADLDALKNDNLPRFANSLAEKSLRYD
                     LTVPFARFVVQHQNELSFPFKRYQIQPVWRADRPQHGRYQEFFQCDADVVGSDSLIYE
                     VDFVLMFDEVLTNLGLPDFSIKINNRKILSGIAEVCGEADKIVTITVAIDKLDKIGVD
                     GVVKELQEKNISNAAIEKIKPLFEMSGSNEEILTQMERYLATSEIGLKGIQELKFVLN
                     AVNALQLKRAKLEFDVTLARGLNYYTGAIFEVKANGVQMGSICGGGRYDDLTGLFGMS
                     GMSGVGISFGADRIYDVLNELNLFPATLTKGVKVLFINFGENEQAYCMQLAHQLRNEN
                     ITCDIYPTAAKLQKQMKYANDTHAEYVALIGEAELETKTITLKNMTSGEQTKFTVEEL
                     INHLK"
     gene            2949..>4258
                     /gene="hutH"
                     /locus_tag="PKN22_06200"
     CDS             2949..>4258
                     /gene="hutH"
                     /locus_tag="PKN22_06200"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNS42332.1"
                     /translation="MNTIIIDRKWLRLEELEAILNTPSHLQLDEATKQEIQKCRDFLD
                     KKVESSTELIYGVNTGFGSLCNTAVSKEDLSTLQSNLVRSHACGMGEMVPFEIVKRML
                     LLKVLGLSHGKSGVQVQTVERLIYFFNNNILPVVYQQGSLGASGDLAPLAHLCLPLLG
                     EGEVLHNGTIRPTSDVYAELNLQPIVLQSKEGLALLNGTQFMSAYASYAVANCFKLWH
                     QFNLIGAMSLEAYDGRLNPFNDNVNSIRNQKGQIETANQFRSILDGSQLIARHKEHVQ
                     DPYSFRCIPQVHGASLDTFKHCAKIVEREINAVTDNPTVFPEDDEVVSAGNFHGQPLA
                     LTMDFMAIAIAEMGSISERRVYKLISGTRGLPAFLVANPGLNSGFMIPQYTCASIVSQ
                     NKQLCTPASVDTIDSSNGQEDHVSMGANAATKLYRVIENCYSIQG"
BASE COUNT         1318 a          717 c          889 g         1334 t
ORIGIN      
        1 gccattgtta tctgtaccag taaccgtgta agttgtactt gctgttgcag ttgtggtaac
       61 tgttgctcca gttgttgcac ttaaatttgt tccaggcgac catgaataag tagttgcgcc
      121 atttgctgtt aatgttgcag aaccgttttc gcaatattca ccattagtta cagtgatggt
      181 tgggtttgga tttaccgtta cgcttattgt tgctgaagtt gcacattgcc ctccatcagg
      241 tgtaaatgta taggttccac ttgctgtgtt gctaattgtt gcgggactcc aagtacccgt
      301 aattccattg gttgaagtgg ttggcaacgc aggtgctaca tctccattac aaattggtga
      361 aatagggtta aatgttggtg taaccgatgg aacggtaacc gtaacttgcg ctgttcctga
      421 acaaccatgc tgtgtatctg taaccgttac agtatatgtt gttgtttgtg ttggagttgc
      481 ggttggatcg ggtactgttg tgctactcaa accatctggt ggcgaccaag aataggttaa
      541 agttcctcca gagcttcctc cacaattaac acgaacagtg taatcctcaa tttcatcact
      601 aaatgtataa ctacctgttc cagaagtata tccggcgtaa ggagcttcta tacctactgg
      661 atttgtataa gtacacccac catctgaagc tacaaaaggt gcattgtctt taaagcggat
      721 gcgcattcta taagctccat aaggttgacc ggctggaacg gtaattggaa tgtttgttaa
      781 aatgatatta ggatgattat ttattgcagg actggtataa acaacttcgg acgcattttc
      841 aaaagtacca ttgttgttcc aatctaccca tactttgcag aaataaggtt ggttattata
      901 caacgatttg gcagtaatat tcaaataaaa tgtatttcca acacatactt caacgtattg
      961 tgatgtaaag tcactcaacc aaattgttga ccaagcattt ggtcccattg gatctatgtg
     1021 accaacatca accaacgtat tggggttgtt aatgttggtt gtaccacctg ttgttgttac
     1081 cgtgttaatg taaattcccc ctccttgtgt taaagaatta ctttgaaaac gtgctgtatg
     1141 catacacgta ggtgccaaag tataatcggg agtagggttg cttgcaggtc cacctaaatt
     1201 agcactacca ccacaaggaa tggtaatatc attgccggca tttgccgtag gaggacattg
     1261 agcatgtagt gtagatgttg tagtgattag tgtgattgat ggaataaaaa gggcgccaaa
     1321 aattagtttt aacaaccctt ttgcatgatt tatttcattc atcacaaaaa agttatgtag
     1381 tttattctta gacgtaaaga aaagaaaaag gttgctaatt atctaaaaaa aagttgattt
     1441 ttttgttaaa agttatattt ttcaataata gcatgttaat aaatgttttt ttaacgtaac
     1501 acaattgtaa attaaacgat taataaattt cagtaaaaac gggtatttgc gctttttttg
     1561 tagttttgta gaaaaataaa ttcaatggct caaaaaccat ccattccaaa gggaacaaga
     1621 gattttttac cgatagaagt agcacgaaga aactacttgt tcaacaccat taaagaagtt
     1681 tataaaaaat atggatttgc tccaattgaa acgccttcgt ttgaattatc atccacttta
     1741 ttggggaaat atggcgaaga aggtgaccgc ctaattttta gaatactcaa ttcgggtgaa
     1801 aaaatgaaaa aagcggattt agacgctttg aaaaacgata atttaccccg ttttgccaat
     1861 tctttggctg aaaaatcatt gcgttacgat ttaacggttc cttttgcgcg ttttgtggtg
     1921 caacaccaaa atgagttgtc gtttccattt aaacgctatc aaattcaacc ggtttggcgt
     1981 gctgatcgtc ctcaacacgg tcgctaccaa gaattttttc aatgcgatgc cgatgtggtt
     2041 ggtagtgatt ctttgattta tgaggttgat tttgttttga tgtttgatga ggtgttgacc
     2101 aatttaggat tgcccgattt ttcaattaaa atcaataacc gtaaaatttt gtcgggaatt
     2161 gccgaagtat gtggcgaagc tgataagatt gttaccatca ccgtagccat cgataaattg
     2221 gataaaattg gtgttgatgg tgtggtaaaa gaattgcaag aaaaaaatat tagcaatgct
     2281 gccattgaaa aaataaaacc gctgtttgaa atgtcgggtt ctaatgaaga aattttaacc
     2341 caaatggaac gttatttggc aacaagcgaa ataggtttaa aaggaattca agaacttaaa
     2401 tttgttttaa atgctgtaaa tgctttgcag ttaaaacgtg caaaattaga atttgatgta
     2461 acacttgccc gtggattaaa ctactatacc ggagctattt ttgaagtaaa agctaacggt
     2521 gtgcaaatgg gaagtatttg tggtggtgga cgctacgacg atttaacagg tttgtttgga
     2581 atgagcggaa tgtcgggggt aggtatttct tttggtgccg acagaattta tgatgtactc
     2641 aatgaattga accttttccc agcaactctt acaaaaggtg taaaagtgtt gtttatcaat
     2701 tttggagaaa acgaacaagc ttattgtatg caattggcac atcaattaag aaatgaaaac
     2761 ataacgtgcg acatttatcc aacagctgct aaattgcaaa aacaaatgaa atatgccaac
     2821 gatacccatg cagaatatgt tgcattaatt ggagaagctg aattggaaac aaaaactatt
     2881 actttgaaaa acatgacttc tggtgaacaa acaaaattta cagtagaaga attaataaat
     2941 catttgaaat gaacacaata atcatcgata gaaagtggtt gcgtttagaa gagttagaag
     3001 caattttaaa tacaccatct cacctacaat tagacgaagc aacaaagcaa gaaattcaaa
     3061 aatgccgtga tttcttggat aaaaaagtgg aaagctcaac cgaacttatt tacggagtaa
     3121 ataccggatt tggttcgttg tgcaacactg ccgtttctaa agaagatttg tcaactttac
     3181 aatccaattt ggtgcgctca catgcttgtg gtatgggaga aatggtaccg tttgagattg
     3241 taaaacgcat gttgttgcta aaagtattgg gcttgtcgca tggaaaatcg ggtgtgcaag
     3301 tgcaaaccgt tgagcgttta atctattttt tcaataacaa tattttgccc gttgtttacc
     3361 aacaaggtag cttgggagca tctggcgatt tggcaccatt ggctcatttg tgtttgcctc
     3421 ttttaggcga aggcgaagta ttgcataatg gaaccattcg tccaacaagt gacgtttatg
     3481 cagaattgaa tttacaaccc attgttttgc aatcaaaaga agggctagcc ttgttgaacg
     3541 gtacccaatt tatgagtgct tatgcgtcgt atgcagttgc caattgtttc aaattgtggc
     3601 atcaattcaa tttaattgga gccatgtcat tagaagctta cgatggtcgt ttaaaccctt
     3661 tcaacgataa tgtgaattca attcgcaatc aaaaagggca aatagaaacc gccaatcaat
     3721 tccgttctat tttggatgga agccagttga ttgctcgcca caaagaacat gtgcaagatc
     3781 cttattcgtt ccgttgcatc ccgcaagtac atggagcatc gttggatacg tttaaacatt
     3841 gtgctaaaat cgtagaacgt gaaattaatg ctgtaacgga taatccaacc gtttttccag
     3901 aagatgacga agtggtttct gcaggaaatt tccatggtca accattggca ttaacaatgg
     3961 attttatggc cattgccatt gcagaaatgg gaagtatttc tgaacgtcgt gtgtataaat
     4021 tgatttcagg aacaagagga ttgccagcat ttttggttgc taacccaggt ttaaattctg
     4081 gatttatgat tcctcaatat acttgtgctt ctattgttag ccaaaacaag caattgtgca
     4141 cacctgcaag tgtagatacg attgactcct ctaacggaca agaagaccac gtatcgatgg
     4201 gagcaaatgc ggctactaaa ttgtaccgtg ttattgaaaa ttgctattct attcaagg
//
LOCUS       DAOQVQ010000168         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23390_length_4237_cov_3.927547, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000168 DAOQVQ010000000
VERSION     DAOQVQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23390_length_4237_cov_3.927547"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PKN22_06205"
     CDS             <1..1761
                     /locus_tag="PKN22_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNS42333.1"
                     /translation="AIADSGQWIVDTKIPEITAANLEYKNIEYWQDSLYFVQKAQGWG
                     GDSLFVVRTTGPATVINLSTWGEINSLQIIDNSLVMNGNGLMIQFNSNYTTKFSIQKY
                     NGTENANINAVVRYKDLLWFADAATGLVKRNIDGSCEKISMKGTARTSYYSMDWSEGV
                     MAVTPGALIVNNYASYTQPGLMLFEDENWSYVDQNTNAKIELNKTFDLVGVSFNPKNK
                     NQIAVGGASGTPLLLVDKSSLTVTDTFGVYNSTLTQEGDGRAVISSLSYDDDGNLWMI
                     NKGNSKPLKLLTPDGQWYEFSLGATATNKTTRKLFCDYNGIVWTSIFNGGLVGYHPGS
                     SVSSASDDQLIVLNSGEYTGKLPSNVVTAIAMDFDNRLWIGTDSGFGILYSPENAFTA
                     GAGNYNVQRPKIDVNGEVDYILGSLYITDIEVDGGNRKWIATVNSGMILLSADGLTVI
                     KHFTTENSPLISNSIMDLEIDQNTGEIFIVTDRGLMSYRGDASYENEKYDDVKIFPNP
                     ARPDFDGLITIQGIRYDSDIKVTDVAGNVVYKTTSNGGTATWNGKNFNGEKVASGVYM
                     FWTASNIHKGRYVGKVVVVN"
     gene            1764..2450
                     /gene="recO"
                     /locus_tag="PKN22_06210"
     CDS             1764..2450
                     /gene="recO"
                     /locus_tag="PKN22_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNS42334.1"
                     /translation="MKQTDLGLFLHRIAYSESSLITTFYTQNNGIQKLVFQGAKKKKT
                     NLFPLAICELTYYHRPDSELGKLTQVDAVVPLYDILLNPVKSLIAFFIADIVKQTLKT
                     NEKEQEIFDFLVKTTKNLENSSDLTLFPLIFLAEYTTYIGIQPHFDTTEQPNYFNLKE
                     GEFHSDFRPGEWCEEGENAQQLYQLFSQQTCLPTFKKKGLELLLNYYQLHIPRFDVDN
                     SLAIIREVLL"
     gene            2519..>4237
                     /locus_tag="PKN22_06215"
     CDS             2519..>4237
                     /locus_tag="PKN22_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNS42335.1"
                     /translation="MKINFLITFLVVWTATISVAQKQLTVMDESGMEPMPFVKVFPNV
                     GQPFYTNVEGTAIVGDSVESILLSFAGYQDTTVFVKDVNAITVKMQTEAKSFNEVVIL
                     PGVNPALRIINNTIENRKLNHPLKNDAFTYDSYSKFVFDVDTALQNKIAKMPIDPKDT
                     VTNMLRKQHVFLLESATERKFIPPARDKETITAYKISGLNNPLFSSFAQTTQSFHFYD
                     NQFELLQSKYLSPIAFGAPSKYYYLIQDTTIVGNDTTFIISFRPRPETSVESMKGFLF
                     INTNGFALEKVIAEPANDTTGFKIKIIQEYQFIDGKKWFPINLKTIGELKSFQISAGK
                     EKGYLVGKGTTYISNVKINPEDMKKSGFNNINLTTELNAGKKSESEWNALRKDSLTTR
                     DANTYHVLDSVSKANNFDRKINTLIALMTGKINVGPVAFPINRVINYNMHEGYRLGLG
                     LETSERLMKNITVGGYFAWGTRDKDWKYGGYSVFHIYKRMGMSIGLKFQQDVLNRGSN
                     ELSNSGWDLTSPMLFTDFYQRYMDRQRLAEINYTIAPLGNLSLNLIGNYQRIDFTHKY
                     QFTKVDG"
BASE COUNT         1396 a          755 c          832 g         1254 t
ORIGIN      
        1 gccatagccg attctggtca gtggattgtt gatacaaaaa tacctgaaat tacagcagct
       61 aatttagaat acaaaaacat tgaatattgg caagatagcc tgtattttgt tcaaaaagca
      121 caaggttggg gaggtgattc tctttttgtt gttcgaacaa cgggacctgc aacagttatc
      181 aacttgagta cgtggggaga aattaactct ttgcaaataa ttgataattc acttgtaatg
      241 aacggtaatg ggttgatgat tcaatttaat tccaattaca ctacaaaatt tagcattcaa
      301 aaatacaacg gaactgaaaa tgcaaatatc aatgcagtag ttcgctacaa agatttgttg
      361 tggtttgccg atgccgcaac ggggttggta aaacgtaaca ttgacggtag ttgtgaaaaa
      421 atcagcatga aaggaacggc aagaacatcc tattattcaa tggattggag tgaaggtgtt
      481 atggctgtaa ctcctggggc tttgattgtc aacaattatg cttcttacac gcaaccgggt
      541 ttgatgttgt ttgaagatga aaattggtcg tatgtggatc aaaataccaa cgcaaaaata
      601 gagctcaaca aaacgtttga cttggttggc gtttctttca atcctaaaaa taaaaatcaa
      661 attgctgttg gtggggcttc gggtacgccg ttacttttag ttgacaaaag ttcattaacc
      721 gttacagata cttttggtgt ttacaactcg actttgacac aagaaggaga cggaagagct
      781 gtcatctcat cactttccta cgatgatgat ggcaatttat ggatgattaa caaaggtaat
      841 agcaagcctt taaaattgtt aactcctgat gggcaatggt acgagtttag tttaggagca
      901 actgctacta ataaaaccac ccgtaagctt ttttgtgatt acaacggcat tgtgtggact
      961 tccattttca acggtggatt ggtgggttat catcccggtt cttctgtaag ttcagcttct
     1021 gatgatcaat tgattgtact caattcagga gaatatacgg gtaaactacc atccaatgtt
     1081 gtaacagcca ttgccatgga ttttgataat cgattgtgga ttggaaccga cagtggtttt
     1141 ggaattttat actcgccaga aaatgctttt acagcgggcg caggaaatta caacgtgcaa
     1201 cgaccaaaaa ttgatgttaa cggcgaagta gattacatcc tcggaagttt atacatcaca
     1261 gatattgaag tggatggtgg caacagaaag tggattgcaa ccgtcaattc gggaatgata
     1321 ttgctttctg ctgatggact aacagttatt aaacatttta cgaccgaaaa ttcgcccttg
     1381 atttccaatt ctatcatgga tttagaaatt gatcaaaaca caggtgaaat ttttattgtt
     1441 accgaccgcg gattgatgtc gtatagaggt gatgcatcgt atgaaaatga aaaatacgat
     1501 gacgtaaaaa tcttcccaaa tcctgctcgc cccgattttg atggactgat aaccattcaa
     1561 ggcattcgtt acgattcgga tataaaagtg accgatgtag ctgggaatgt agtgtataaa
     1621 acgacatcca atggtggaac ggcaacatgg aacggcaaga attttaatgg agaaaaagtg
     1681 gcttcggggg tttacatgtt ttggaccgca tccaacattc acaaaggacg ctacgtgggc
     1741 aaagttgttg tagtaaacta acaatgaaac aaacagatct gggtttattc ttgcaccgca
     1801 ttgcctactc tgaatcgagt ttaattacca ctttttatac ccaaaataac ggtatacaaa
     1861 aattggtgtt tcaaggggca aagaagaaaa aaaccaattt gtttccattg gcaatttgtg
     1921 aattaaccta ttaccatcgt cccgattctg aattaggaaa attaacacaa gtggatgcag
     1981 tagttccgtt gtacgacatt ctgctaaatc ccgtaaaaag tttgatagca ttttttattg
     2041 ctgacatagt aaaacaaaca ttaaaaacaa acgaaaaaga gcaagaaatc tttgattttt
     2101 tagtaaaaac aactaaaaat cttgaaaatt catccgattt aacccttttt ccactcattt
     2161 ttcttgcaga atacaccaca tacatcggca tacaacccca ttttgacaca acagaacaac
     2221 ccaactattt caatttaaaa gaaggtgaat ttcactccga ttttcgtcct ggcgaatggt
     2281 gcgaagaagg tgaaaatgca caacagttat atcaactttt tagccaacaa acgtgtttgc
     2341 ccacttttaa gaaaaaagga ttggaattgt tgctcaacta ttatcaattg catattcccc
     2401 gttttgatgt tgacaattcg ttggccatca ttcgagaggt gttgctgtag atttaaaaaa
     2461 acaagccaat tgttttagta tatttgacaa tttatagaaa aaaacaacaa tttaaaagat
     2521 gaaaatcaac tttttaatta catttttagt tgtatggaca gccactattt ccgttgcaca
     2581 aaagcaactg accgtaatgg atgaatcggg aatggagcct atgccgtttg taaaagtttt
     2641 ccccaatgtt ggtcagccat tttataccaa tgttgaagga actgcaatag ttggagacag
     2701 tgttgaatct attttattat cctttgcggg ctaccaagac acaactgttt ttgttaaaga
     2761 tgtgaacgcc atcactgtca aaatgcaaac ggaggcaaaa tcgtttaatg aagtggttat
     2821 tttaccggga gttaaccctg ctttgcgcat catcaacaac accattgaaa atagaaagct
     2881 caaccatcca ctcaaaaacg atgcttttac ctatgacagc tattcaaaat ttgtgtttga
     2941 tgttgacacg gccttgcaaa ataaaattgc taaaatgccc atcgatccaa aggatacggt
     3001 taccaacatg ttgcgcaaac aacatgtgtt tttattggaa agtgccaccg aacgtaaatt
     3061 cattccacca gcacgtgata aagaaaccat taccgcctat aaaatttctg gtttaaacaa
     3121 tcccttgttt tcttcgtttg cacaaaccac gcaatctttt catttttacg acaatcagtt
     3181 tgaattgttg caaagcaaat atttgagtcc cattgcattt ggtgctccaa gcaaatatta
     3241 ttacttgata caagacacca ccattgttgg aaacgacacc acatttatta tttcctttcg
     3301 cccacgtcct gaaacatcgg ttgaatccat gaaaggattt ttattcatca acaccaacgg
     3361 gtttgcattg gaaaaagtaa tagcagaacc agccaacgat acaactgggt ttaagattaa
     3421 aattattcaa gaatatcaat ttattgatgg taaaaaatgg tttccaatca atttaaaaac
     3481 tattggtgaa ttaaagagtt ttcagatttc tgctggaaaa gaaaaaggct atttggttgg
     3541 aaagggtacc acctatatct caaatgtaaa aatcaatccc gaagacatga aaaaatcggg
     3601 gttcaacaac atcaacctaa caacagaatt gaatgcaggc aaaaaatcgg aaagcgaatg
     3661 gaatgccttg cgaaaagatt ctttaacaac gcgtgatgcg aatacctatc acgtgttaga
     3721 tagcgttagc aaggcaaata attttgatag aaaaatcaac acattgattg cattgatgac
     3781 tggtaaaatt aatgttggtc cggtagcatt tcctatcaat cgtgtgatta actataacat
     3841 gcacgaaggt taccgattgg gcttaggttt ggaaacgtct gaacgattga tgaaaaacat
     3901 tactgttggc ggttattttg cttggggaac acgtgataaa gattggaaat atggtggtta
     3961 ttctgttttt catatctaca aacgaatggg catgagcatc gggttgaagt ttcagcaaga
     4021 tgttctcaac agaggttcca atgaattgtc aaacagtggg tgggatttaa cctcaccgat
     4081 gttgtttacc gatttctatc agcgctacat ggaccgtcaa cgcttggctg aaattaacta
     4141 caccattgcg ccacttggca atttatcgtt gaatttaatt ggaaattacc aacgtattga
     4201 ttttacccat aaataccaat ttacaaaggt tgacgga
//
LOCUS       DAOQVQ010000169         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23393_length_4237_cov_3.554998, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000169 DAOQVQ010000000
VERSION     DAOQVQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23393_length_4237_cov_3.554998"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="pnuC"
                     /locus_tag="PKN22_06220"
     CDS             complement(<1..407)
                     /gene="pnuC"
                     /locus_tag="PKN22_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNS42336.1"
                     /translation="MEITIFTAMEQFFTNFLQGIHQTSPFEWIAVCCSICYLLLAIKE
                     SIYCWMFAFISSSIYVYIAYSVQLHLDALLQLFYVAMAVVGFVNWNKQKEDKKIKVED
                     TEYDAQFIDLLSKIKVVLKDKIKNLAEFGDFYVG"
     gene            complement(507..1649)
                     /locus_tag="PKN22_06225"
     CDS             complement(507..1649)
                     /locus_tag="PKN22_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNS42337.1"
                     /translation="MNFELSEEHLAVKAAARDFAQNVLKPGVIERDRDQRFPAEEIKQ
                     LAELGFMGMMVDPKYGGSGMDALSYVLAMEEISKVDASTSVCMSVNNSLVCWGLEEYG
                     TEEQKQKYLVPLAKGEKIGAFCLSEPEAGSDATSQRTTAVDMGDHYLVNGVKNWITNG
                     SSASTYLVICQTHPEKGHKGINALIIERGMEGFVVGAKEDKMGIRGSDTHTLMFNNVK
                     VPKENRIGEDGFGFTFAMKVLSGGRIGIASQALGIAAGAFELATAYAKERAAFGKPIG
                     QHQAIAFKLADMATDIEAARLLVYRAALDKDAHRNYDQSSAMAKLFASQVAMKHTVEA
                     VQIHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQKIVISRNVLKD"
     gene            1705..2796
                     /locus_tag="PKN22_06230"
     CDS             1705..2796
                     /locus_tag="PKN22_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNS42338.1"
                     /translation="MQQKVNRSYKIIGLMSGTSLDGLDICCANFSLSESNDWSFEIEA
                     TQFITYPTELLKQLQQATILSGLEFMLLDKNLGVFYGEKVNAFLQEKKIDGSAIDAIA
                     SHGQTIFHQPEIGFTCQIGCGQSIATSTGIQTINDFRKKDVLHGGQGAPLVPIGDKLL
                     FSSFADAFLNIGGFANMTKIDRQNRVVAFDICPANIAINHYTQQLHLAFDKDGAIAKN
                     ARIDASLLIQLNDLEIYKQQQPPSLGWEWVEATVLPLVEATQLTVEEKIATLTEHAAY
                     QIAQRLNNTASQNVFVTGGGAKNQFLMERLAHYFTGKVVVPSTEIIDFKEALIFGFLG
                     ALFLENTPNCLPEVTGASKAVVGGVLHRP"
     gene            complement(2909..3685)
                     /locus_tag="PKN22_06235"
     CDS             complement(2909..3685)
                     /locus_tag="PKN22_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNS42339.1"
                     /translation="MNDQLQIIPTDLLEIYSQQVPKTLQSSFEALEDAEISTDTFSFY
                     TSVSSVFSSKIEGEDIDLDSYIKHKKFGVEFLPDYTKKIDDLYNAYTFAKTNELNQKN
                     ISEAHKILSKSIVATNWQGKLRSQNMYVTTPDGRIEYVATSPFDLENEMNKLYSDIAI
                     LLNEKLSIEEVFFFASLIHLVFVKIHPWNDGNGRTARLLEKWFLAQKLGHNAWFIQSE
                     KMYYSQHNHYYKNIRLLGLEYTELDYSQALPFLLMLPASL"
     gene            complement(3943..>4237)
                     /gene="glyA"
                     /locus_tag="PKN22_06240"
     CDS             complement(3943..>4237)
                     /gene="glyA"
                     /locus_tag="PKN22_06240"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386457.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNS42340.1"
                     /translation="DLRSKGLNGKDAENALVKADITVNKNMVPFDTESPFVTSGIRLG
                     TPALTSRGVKDNEIPTIVDLIDKVLMNMNNDTVIESVKKDVIALMGNRKLHVG"
BASE COUNT         1318 a          810 c          712 g         1397 t
ORIGIN      
        1 ccaacgtaaa aatccccgaa ttcggccaga ttttttattt tatcctttaa aacaactttg
       61 atttttgaaa gtaaatcgat aaattgagca tcatattcag tatcttcaac ctttattttt
      121 ttatcttctt tttgtttgtt ccaattaaca aaaccaacaa cggccattgc tacgtaaaat
      181 agttgcaata aagcatccaa atggagttgc accgaatagg caatgtacac gtaaatagac
      241 gaactgataa aagcaaacat ccaacaataa atactttctt ttatggctaa tagcaaataa
      301 caaatgctgc aacatacagc tatccattca aatggagatg tttggtgaat tccttgtaaa
      361 aaattggtaa aaaattgttc cattgctgta aaaatagtta tttccattaa agtggatatg
      421 atttttttag cttttagaaa atcaatgttg caaaacaaaa aaagctgcct tcaacagaca
      481 gcttttcatt atgaaaaaga gaaagattaa tctttcaata catttctaga aatcacgatt
      541 ttttgaactt cagaagttcc ctcgtaaatt tgtgtaattt tcgcatcgcg catcaaacgc
      601 tcaacgtggt attctttaac gtaaccgtat ccaccgtgga tttgtaccgc ttcaactgtg
      661 tgtttcattg ccacttgaga agcaaacaat tttgccattg cagatgattg atcgtagttt
      721 ctgtgtgcat ctttatccag cgcagcacgg taaaccaaca aacgagcagc ctcgatgtca
      781 gtagccatat ctgccaattt gaatgcgatt gcttggtgtt gaccaattgg ttttccaaaa
      841 gcagctcttt ctttagcata agcagttgcc aattcaaatg cacctgcagc aattcctaaa
      901 gcttgagaag caataccaat acgtccgcca gaaagtactt tcattgcaaa tgtaaatcca
      961 aaaccatcct caccaatacg gttttctttt ggaactttaa cgttgttgaa catcaaagta
     1021 tgtgtatcac ttccgcgaat ccccatttta tcttcttttg ctcccacaac aaagccttcc
     1081 atgcctcttt caataatcaa tgcattgatt cctttgtgtc ctttttctgg gtgtgtttgg
     1141 caaataacta aataagttga agccgacgaa ccattggtaa tccagttttt aacaccgttt
     1201 accaagtaat ggtcgcccat atcaacagct gtagtgcgtt gtgaagttgc atccgaacca
     1261 gcttctggct ctgacaaaca aaatgcaccg attttttcgc cttttgcaag tggaactaag
     1321 tatttttgtt tttgctcttc tgttccgtat tcttccaatc cccaacaaac caatgagttg
     1381 ttaacggaca tacaaacaga tgttgaagca tccacttttg aaatttcttc cattgccaat
     1441 acatacgaaa gggcgtccat accgctacct ccatattttg gatctaccat cattcccata
     1501 aatccaagct ctgctaattg cttgatttct tctgctggaa aacgttgatc tctatcgcgc
     1561 tcaattactc ctggttttaa tacattttga gcaaaatctc ttgcagctgc ttttactgct
     1621 aaatgttctt ctgataattc aaaattcata gttatatttg gtttcatttt cgactgaatg
     1681 caaatttaaa cattttataa aattatgcaa cagaaagtta acagaagcta taaaataatc
     1741 ggactcatgt cggggacttc gcttgatgga ttggatattt gttgtgcaaa cttctcatta
     1801 tcagaaagta atgattggag ttttgaaata gaggctacac aatttattac ctatccaacc
     1861 gaattattaa aacaattgca gcaagcaacc atactttctg ggcttgaatt tatgttgttg
     1921 gataaaaatc taggtgtatt ttatggtgaa aaagtaaacg catttttgca agaaaagaaa
     1981 attgatggtt cagcgattga cgcaatagca tctcacggac aaaccatttt tcatcaacca
     2041 gaaattggtt ttacttgcca aattggttgt ggtcaatcca ttgcaactag tacaggtatt
     2101 caaaccatca atgattttag aaaaaaagat gtattgcacg gcggacaagg agccccgttg
     2161 gttccaattg gcgataaatt gcttttttca tcttttgcag atgccttttt aaacattgga
     2221 gggtttgcca atatgacaaa aattgatcgt caaaataggg tagtggcttt tgatatttgt
     2281 ccagccaaca ttgcaattaa tcattacacc caacaactcc atttagcatt tgataaagat
     2341 ggcgcaattg ccaaaaatgc acgtattgat gcatcgctat tgatacaatt gaatgatttg
     2401 gagatttata agcaacaaca acctccttct ttgggatggg agtgggtaga agctaccgtt
     2461 ttaccacttg ttgaagctac tcagctaact gttgaagaaa aaatagctac gttaaccgaa
     2521 catgcagcct atcaaattgc gcaacgattg aacaacacag cttcacaaaa cgtttttgta
     2581 actgggggag gggcaaaaaa tcaattttta atggagcgat tggcacatta tttcactggc
     2641 aaagtggtgg tgccatccac cgaaattatt gactttaaag aagcgttgat ttttgggttt
     2701 ttaggtgctt tgtttttgga aaacaccccc aattgtttac ccgaagtaac aggcgcatcg
     2761 aaagcagttg ttggaggagt tttgcaccgt ccataatgtt tctcgtcatg aaaaggtggt
     2821 gaggataaac actaaacttc aaccgaagat aataaattta aagaagagat tgaacttgct
     2881 tccctccatc attttctcat agatttacct atagagaagc aggtaacatg agtaaaaagg
     2941 gcaaagcttg tgaataatct aattctgtat actccaaacc taacaaacga atatttttgt
     3001 agtaatggtt gtgttgactg taatacattt tttcactttg aataaaccat gcattgtgtc
     3061 ccagtttttg ggctaaaaac catttctcaa gcaaccttgc agttctgccg ttgccatcat
     3121 tccaaggatg gattttcaca aaaactaaat gaatgagtga agcgaaaaag aaaacttctt
     3181 ctatacttag tttttcattt agcaaaattg ctatatcgct ataaagcttg ttcatttcat
     3241 tttccaaatc aaacggtgaa gttgctacat attctatacg tccgtcaggt gttgtgacat
     3301 acatattttg agaacgcaat ttcccctgcc aatttgtggc tacaatgctt ttactcaata
     3361 ttttatgagc ttctgaaatg ttcttttgat taagttcatt ggttttagca aaagtatagg
     3421 cattgtataa atcgtcaatc ttttttgtat aatctggcaa aaactcaacc ccaaattttt
     3481 tatgtttgat gtagctatcc aaatctatat cctcgccctc tattttacta ctgaaaacag
     3541 atgacactga agtataaaaa ctaaatgtat cggttgaaat ttcggcatct tccaaagcct
     3601 caaaactcga ttgcaaagtt ttgggaactt gttggctata aatttcaagc aaatctgttg
     3661 gaattatttg taattgatca ttcactttgc aaagttgttt tcagttctta tttttattat
     3721 gtactcgccc caatttgctc ccattgattt gatttttaca aaagtaataa tataattatt
     3781 cctgaacaat gtctaaaatt tctttgggtg acatggatta gtgggaatgt ataatcaaat
     3841 gtacttaaac ttactttaac tggagctcaa attctattat aaaacaacaa aaaggctgct
     3901 cagtagtttg aacagcctct tggtattttt atgttgaata gcttatccaa cgtgtaattt
     3961 tctgttgccc atcaatgcga tgacatcttt tttcaccgat tcaatcactg tatcgttgtt
     4021 catgttcatc aacactttgt caattaaatc tacaatggtt ggaatttcat tgtccttcac
     4081 accacgagaa gttaaagcag gcgttcctaa acgaatacca gaagtaacaa atggcgattc
     4141 agtatcaaac ggaaccatgt ttttgttcac tgtaatatcg gctttaacca atgcattttc
     4201 agcatcttta ccgttcaatc ctttagagcg caagtca
//
LOCUS       DAOQVQ010000170         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23844_length_4177_cov_3.632460, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000170 DAOQVQ010000000
VERSION     DAOQVQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23844_length_4177_cov_3.632460"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PKN22_06245"
     CDS             complement(<1..786)
                     /locus_tag="PKN22_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42341.1"
                     /translation="MNFTRLAAGMLVLTLPLVACKKEGCTDATATNYNEKAKKDDGSC
                     EYAIAGELVGSITGTRTLEAGISYTITGPVIVEDGGVLNIPAGTVIKAKKGFNNYILV
                     LQGGKINVNGTAEKPVKITADIANAQAGHWGGLIINGRAPLAGGTIGSTEISSAHQYG
                     GTIANDNSGSITYLILEYTGARSSADVEHNGLTLNGVGSGTVIENVFIPHGSDDGIEF
                     FGGTVNVKNLLVVNSDDDMFDFTQGYAGTLENAYGIWEVGHTSS"
     gene            complement(905..3601)
                     /locus_tag="PKN22_06250"
     CDS             complement(905..3601)
                     /locus_tag="PKN22_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42342.1"
                     /translation="MKIKGIYHIFTLLICSLFTTISFSQNASIQGKITDAQTNEGLMG
                     AKVQLINTTKGAICDLDGNYVIANLEPGSYIVSVSLISYQTHNSDKITVKAGETVTFD
                     AALNVDEVSIEEVEIVAKVNRESEVGLLLEQKKSMLATQSIGASELSRKGIGDAESAV
                     ASVSGISKQEGVKNVFVRGLGDRYNSTLLNGLPVPSEDPEYKNIALALFETDIIKNIG
                     VDKVFSSSSHSDVGGAIITIQSKELQDNYEFGVSVSTGINSRAIGTNFKKSDGFNYFG
                     ISNISQPKNEEFNFNNKLDPQAVKFPMNESYRISGGKKFDFKKSSLSLFGVATHASDY
                     SYTEEIVRNTTTDGTIYQDQIGKKYSGKKSQLALANINYTIANAHSISYNFMLLHATN
                     HYVGEFLGKHAEKFQDGATDQGYLRRQQINDNLLLTNQLLTSWKLSEKWRINLDAAVN
                     QIKGSEPDRRENYLSQKADGSYGLTGSNRQKRFYSRLEENDYNAKLEVRYQLNDKQKS
                     NNSALSFGYNGRFSSNHFEATEYNFSAVSGKLTIDNLSLDNMYNAANFQEERFTMTQS
                     FPSSYNVSRNNHSAFALASYQFSSKWSGALGFKYDFVNLSVLYDVPGQKGMDSIRQAF
                     YLPSVNLKYDITEKHSLRFGASKTYTLPQSKEISPYQYVNIGFTSEGNHNLRHSDNYN
                     ADLKWDYYLTPSEIITFGVFYKYIVNPIGRVDKGNSAGLLTYDNIGKAAQVFGAEFEL
                     RKTIFHLKNNNFTKENKLTIGTNVSYIFTSTTLNLTNTPERNSQLEGASPLLINADLT
                     YNFSKNEKGLTTSLVFNYFSDRIFTIGTMGYNDIIEKGVPTLDIVLAGKISKRIGLKL
                     KASNVLDPNFRLTRKVAATGETVLLNQYKKGVNISLGLSFNL"
     gene            complement(3815..>4177)
                     /locus_tag="PKN22_06255"
     CDS             complement(3815..>4177)
                     /locus_tag="PKN22_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNS42343.1"
                     /translation="SFNKHKGMEESTIKSEMKTRQSVSLTPLAIPMLAGPGTISLLIS
                     LQKQHTQFQEQLIIIGAIFIVCLLTYFLLKESHYIVKLLGASGLNAISRIIGFIVIAI
                     GIEYISSSVIKIVSSLLH"
BASE COUNT         1203 a          789 c          726 g         1459 t
ORIGIN      
        1 tgaactggtg tgtccaactt cccaaatacc ataagcattt tctaaagttc cagcatatcc
       61 ttgtgtgaaa tcaaacatat catcgtcgga gtttacaacc aacaagtttt ttacgttaac
      121 agttccacca aaaaactcaa taccatcgtc agatccatga ggaatgaaca cgttttcaat
      181 tacagttccg cttccaactc cgtttaatgt caaaccattg tgttccacat ctgcacttga
      241 acgtgctcca gtgtattcta aaattaaata ggtaatgctt ccagagttgt cgtttgcaat
      301 tgttccacca tattgatgag ccgagctaat ttctgtacta ccaattgtac caccagccaa
      361 aggagctcta ccattgataa tcaatccacc ccagtgtcct gcttgtgcat ttgcaatatc
      421 agctgttatt tttactggtt tttcagctgt tccgttaaca ttgattttac caccttgtaa
      481 taccaaaata taattgttaa atcctttttt tgctttaatt actgtaccag caggaatgtt
      541 taacacacca ccgtcttcta caatcactgg acctgtaatt gtgtagctaa tacctgcttc
      601 caatgttctt gtacctgtga tgctaccaac caattcgcca gcaattgcat attcacaaga
      661 accatcgtct ttttttgctt tttcgttgta atttgtagct gttgcatccg tacaaccttc
      721 ttttttacaa gccactaaag gaagtgtaag aacaagcata cctgctgcta atcttgtaaa
      781 attcattttt cttgttttca tttttttaat ttttgtttaa acttttatcc tttttttaca
      841 ctacaaatgt cgagcttcca tattaccttc tcgttgctct ggtattgaga ttttgttacg
      901 ttttttataa gttgaaacta agtcctaagc taatgttgac tccttttttg tattggttca
      961 acaaaacagt ttcacctgtt gcagcaactt tacgtgttaa tctgaagtta ggatccaata
     1021 cattagaggc ttttagtttc aaaccaatgc gtttgctaat ttttccagcc aatacgatgt
     1081 ctaatgttgg aacacctttt tcaataatgt cgttataacc catggttcca atggtgaaaa
     1141 ttctatcgct gaagtagttg aacactaagg atgttgttaa acctttttcg tttttactga
     1201 aattgtaagt taaatcggca ttgataagca atggagatgc tccttccaat tgtgaattgc
     1261 gctcgggtgt attggttaaa ttcaatgttg tggaagtgaa aatatacgaa acattggttc
     1321 ctattgttaa cttattttct ttagtaaaat tgttgttttt taggtggaaa atggtttttc
     1381 gcaattcaaa ttcagctcca aaaacctgtg cagctttgcc aatgttgtca taagttaata
     1441 aaccagccga atttcctttg tcaacacgtc caatagggtt tacaatgtat ttgtaaaaca
     1501 ccccaaaagt gatgatttcg gaaggagtta agtaataatc ccatttcaaa tcggcattgt
     1561 aattatcgga atggcgcaag ttgtgattac cttcactggt gaaaccaata ttaacgtatt
     1621 ggtaaggtga aatttctttt gattgaggca atgtgtatgt tttacttgct ccaaaacgca
     1681 agctgtgttt ttctgtaatg tcgtatttta aattaacgct tggcaaatag aacgcctgac
     1741 gaatgctgtc cattcccttt tgacctggaa cgtcatacaa cacgctcaaa ttcacaaaat
     1801 catatttgaa acccaaagca ccgctccatt tgcttgaaaa ttgatagctt gccaatgcaa
     1861 atgcagaatg attgttgcgc gatacattgt agctactagg aaaactttgt gtcattgtaa
     1921 aacgctcttc ttgaaaattg gctgcattgt acatgttgtc taacgataaa ttatcgatag
     1981 ttaactttcc agatacagct gagaaattgt attcggtggc ttcaaaatgg ttggatgaga
     2041 aacgaccgtt gtatccaaat gaaagtgcag aattgttgct tttttgtttg tcgttgagtt
     2101 ggtatctaac ctctaatttt gcattataat cgttttcttc taaccttgaa taaaaacgtt
     2161 tttggcgatt actacctgtt aaaccatagc ttccatcggc tttttgagat aaatagtttt
     2221 ctcttctatc gggttccgaa ccttttattt gattaacagc agcatcaagg ttaatgcgcc
     2281 atttttctga aagtttccaa ctggttaaca attgattggt caacaataaa ttgtcgttta
     2341 tttgttggcg acgtaaatac ccttggtcgg tggcaccatc ttgaaacttt tcggcatgtt
     2401 ttcctaaaaa ttcacctacg taatgattgg ttgcgtgcaa caacatgaaa ttgtaagaaa
     2461 tactgtgtgc atttgcaatc gtgtaattga tgtttgccaa tgccaattga cttttctttc
     2521 cactgtattt tttcccaatt tgatcttgat aaattgttcc atctgttgtg gtattgcgca
     2581 caatttcttc ggtataagaa tagtcggagg cgtgcgttgc aacaccaaaa agggataaac
     2641 tacttttctt aaaatcaaat tttttgccac ccgaaatgcg gtagctttca ttcattggaa
     2701 atttgacagc ttgcggatcc aatttattgt taaagttaaa ttcctcgttt ttaggttggc
     2761 taatgttgga tattccaaaa taattgaaac catcggattt tttgaaatta gtaccaattg
     2821 ctcgcgagtt aattcctgtt gaaaccgaaa caccaaattc atagttgtct tgcaactctt
     2881 ttgattggat ggtgattatt gcaccaccta catccgaatg cgatgaagaa gaaaacactt
     2941 tatctacacc aatgtttttt ataatgtctg tttcaaacaa cgccaaagca atgtttttat
     3001 actctgggtc ttcggatgga actggtaatc cgttcaataa ggtggagttg tatcggtcgc
     3061 ccaatcctct cacaaatacg ttttttacgc cttcttgttt tgaaataccc gaaacagagg
     3121 caacagcaga ttctgcatca ccaattcctt ttcgggataa ttcggaggct ccaatgcttt
     3181 gtgtggcaag catggatttt ttttgctcca acaacaaacc aacttccgat tcacggttaa
     3241 cttttgcaac tatttccact tcttcaatag acacttcatc cacgtttagt gctgcatcaa
     3301 acgtaactgt ttcacccgct tttacggtga ttttatctga attgtgagtt tgataggaaa
     3361 tcaacgaaac tgaaacaata taagaacctg gttctaagtt tgcaatgaca taatttccat
     3421 ccaaatcgca aattgcacct ttggttgtgt taatcaattg cacttttgct cccatcaacc
     3481 cttcgttggt ttgtgcatcg gttatttttc cttgaatact agcgttctga ctaaagctaa
     3541 ttgtggtgaa taaactgcaa attagcagtg tgaaaatgtg ataaatacct tttattttca
     3601 tttttttaga atttacttga gttatttttt ctcactgcaa aattctaaac ttgttgttgc
     3661 agttttgtta cgcaaaaact acattttccc taatcttaaa atgaaaatat tacattctta
     3721 caacaagcgt gcgtgggggc ggttttgtgt tgaaaaaggc aggttagtgt agtaaaaagt
     3781 aaagattata aacggctttg ttgcaaaaaa gtaattagtg taaaagcgat gaaacaattt
     3841 ttatcaccga tgaactgatg tattcaattc ctatagcaat aacaataaat ccaatgatgc
     3901 gtgaaatggc attcaacccc gatgcgccca aaagttttac gatgtaatgc gattctttta
     3961 ataggaaata ggtcagtaag cacacaataa aaattgctcc aataataata agttgttcct
     4021 gaaattgggt gtgttgtttt tgcaacgaaa tcaataaaga aatggttcca ggaccagcca
     4081 acatcggaat tgccaatggc gtaagtgaaa ccgattgtcg ggttttcatc tccgatttta
     4141 tggttgattc ttccatccct ttgtgtttgt taaaaga
//
LOCUS       DAOQVQ010000171         4171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23886_length_4171_cov_3.320943, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000171 DAOQVQ010000000
VERSION     DAOQVQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23886_length_4171_cov_3.320943"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKN22_06260"
     CDS             <1..680
                     /locus_tag="PKN22_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNS42344.1"
                     /translation="VWFYAILGIFGVWTSFLLSGVHATISAVLAAFIIPSDVKIKEKV
                     YIEKLQHHLNAFKNIDPKEEIPTLTNEQLEILDELKKDTNEAIPPLQKLEHAMHPAVT
                     FIILPIFAIANAGVSLLDIDPEILLSTNVMLGVALGLLFGKVIGVVGFTWLAAKLKIA
                     QFPTGMNLRNLFGLGLLAAIGFTMSLFVTSLAFSHQEYVMQAKIGIFSASIIGGVLGY
                     LVLKKQH"
     gene            838..2793
                     /gene="gyrB"
                     /locus_tag="PKN22_06265"
     CDS             838..2793
                     /gene="gyrB"
                     /locus_tag="PKN22_06265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNS42345.1"
                     /translation="MSEEIKKNQDYSADNIQILEGLEAVRKRPAMYIGDVGIRGLHHL
                     VYEVVDNSIDEALAGYCDTIDISINEDNSITVKDNGRGIPTAIHSKEKRSALEVVMTV
                     LHAGGKFDKDTYKVSGGLHGVGVSCVNALSTHFRAEVHREGKIFEQEYSIGKPLYSVR
                     EIGTSNVTGTIVTFKPDASIFEDTVYNYDTLAARMRELAYLNKGIRITLTDLREKDDN
                     GNYNSTQFYSEGGLKEFVQYLDRNREALVQDVIYFEGEKDGIPVEVALTYNTSYTENI
                     QAYVNNINTHEGGTHLSGFRRGLTNTLKKYATDSGMTAKEKVEIEGDDFREGLTAVIS
                     VKVAEPQFEGQTKTKLGNREVTAPVSQAVSEMLADYLEEHPNEAKQIVYKVILAAKAR
                     HAARKAREMVQRKNVMSGSGLPGKLADCASKDAAECELFLVEGDSAGGTAKQGRDRHF
                     QAIMPLRGKILNVEKAMQYKIFENEEIKNIYTALGVRIGTEEDSKALNMEKLRYHKLI
                     IMTDADVDGSHIETLILTFLFRYMKPLIELGYVYVATPPLYQVKKGNKSDYAWNDDQR
                     DLLIQQFKGAGADSSVNVQRYKGLGEMNAEQLWETTMNPEGRTLRQITIENAAEADQI
                     FSMLMGDDVPPRREFIEKNAKYAKIDA"
     gene            2905..3876
                     /gene="pfkA"
                     /locus_tag="PKN22_06270"
     CDS             2905..3876
                     /gene="pfkA"
                     /locus_tag="PKN22_06270"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNS42346.1"
                     /translation="MKKIGVLTSGGDAPGMNACIRAITKFCLNHKIECVGIMDGYDGL
                     IHQKAINLGYDEVNNIIQRGGTILGSARSKEFQTVEGRKKAHAFITQLQIDGLIIIGG
                     DGSLTGAKIFSDEFNFPIIGIPGTIDNDILGSDHTIGYDTAVNTVIEAVDKIRDTASS
                     HKRIFFVEVMGRDSGLIALNSAIASGAESVLIPEKNTDIEKLVEDIKEVHKGKRSAII
                     IVAEGDDAGDAVTIMKKVKPLLSDYDLRATVLGHIQRGGNPTAYDRIIATRMGIKAVE
                     LLLENKSKLMIATEGSKLVTRSIDFAEKKSPDTALTKLQIIEQLRTK"
     gene            3878..>4171
                     /gene="tsaE"
                     /locus_tag="PKN22_06275"
     CDS             3878..>4171
                     /gene="tsaE"
                     /locus_tag="PKN22_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNS42347.1"
                     /translation="MQQLITNIQELPAFATQFLDKYSHHRIFLFEAEMGCGKTTFIQE
                     LLRQMGVENPNGSPTYSIVNEYTTPNYGKIYHFDLYRIQDEEEIYDIGIEDMLY"
BASE COUNT         1413 a          719 c          847 g         1192 t
ORIGIN      
        1 gcgtttggtt ttatgctatt ttgggaatct ttggtgtttg gacttcgttt ttattatcgg
       61 gagttcatgc aactatttca gctgtattgg cagcttttat catcccttct gatgtgaaaa
      121 ttaaagaaaa agtgtacatc gaaaaattgc aacatcatct aaatgcattt aaaaatatcg
      181 atcccaaaga agaaattcca acgctaacca acgagcaatt ggaaatttta gacgaattga
      241 aaaaagacac caacgaggcc attcctcctc ttcaaaaatt agaacacgca atgcatccgg
      301 cagtaacatt tatcatactc ccaatttttg ccattgctaa tgcgggtgta tccctattag
      361 acatcgaccc tgaaatttta ttgagcacca acgttatgct cggcgttgcc ctaggtttac
      421 ttttcggaaa agtgattggc gttgttggtt ttacatggtt ggcagccaaa ttgaaaattg
      481 cacaatttcc tactggaatg aaccttagaa acttgtttgg tttaggatta ctagccgcca
      541 ttggatttac catgtcgttg tttgttacat cgctggcatt ttcgcaccaa gaatacgtta
      601 tgcaagccaa gattggtatt ttctcagctt caattattgg aggtgtttta ggttatttgg
      661 tactaaaaaa acaacactaa aaacaacgaa ttaataaaaa gaagacaaaa ataccttctt
      721 cgttaattaa ttgttgataa gtctttttgc aagtcgactt ttttgctgtt attacaactt
      781 ttaattgctt aaatttgcaa gctataataa tatttcagaa caatacaaaa caacacaatg
      841 agtgaagaaa taaaaaagaa tcaagattat tcagcagata atattcaaat tcttgaggga
      901 ttagaagctg taagaaaacg tcctgccatg tatattggtg atgttggaat tcgaggttta
      961 caccatttag tatacgaagt tgtagacaac tctatcgacg aagctttggc tggatattgt
     1021 gatacaattg atatttctat taacgaagat aattccatta ctgtaaaaga taatggtcgt
     1081 ggtattccta cggctattca ctccaaagaa aaacgttcag cgttggaggt tgtaatgaca
     1141 gttttgcacg ccggaggtaa atttgataaa gacacttata aagtatctgg aggtttgcac
     1201 ggtgtgggag tatcttgtgt aaacgcattg tccactcatt ttagagcaga agttcaccgc
     1261 gaaggaaaaa tatttgaaca agaatattca atcggtaaac cattgtattc cgttcgcgaa
     1321 attggtacat ccaacgttac tggaaccatt gtaactttca aaccagacgc ctcaattttc
     1381 gaagatactg tttacaatta cgatacatta gcagccagaa tgcgtgaatt agcgtatttg
     1441 aacaaaggaa ttcgtatcac cttaacagat ttaagagaaa aagacgataa cggaaattat
     1501 aattcaactc agttttattc ggaaggtgga ttgaaagaat ttgttcaata cttggacaga
     1561 aaccgtgaag cattggtgca agatgtgatt tattttgaag gtgaaaaaga cggaattccg
     1621 gtagaagttg ccttgaccta caacacatcg tatacagaaa atattcaagc gtatgtaaac
     1681 aacatcaata ctcatgaagg tggaacacac ttatcagggt ttagaagagg tttaaccaat
     1741 accttgaaaa aatatgcaac tgattctgga atgactgcaa aagaaaaagt agaaattgaa
     1801 ggcgacgact tccgtgaggg gttaacagcc gtaatttctg ttaaagttgc agaaccacag
     1861 tttgaggggc aaacaaaaac aaaattagga aaccgtgaag ttactgctcc tgtgagccag
     1921 gcggtttctg aaatgttggc ggattatttg gaagaacatc caaacgaagc caagcaaatt
     1981 gtttacaaag ttattttggc cgcaaaagct cgtcatgctg ctcgtaaagc acgtgaaatg
     2041 gtgcaacgta aaaacgtgat gtcgggttct ggacttccag gaaaattggc tgattgtgct
     2101 tctaaagatg ctgctgagtg tgagttattc cttgtcgagg gagactctgc gggtggtact
     2161 gcaaaacaag gtcgcgaccg ccacttccaa gctatcatgc cgttgagagg taaaatcttg
     2221 aacgttgaaa aagcaatgca atacaagatt tttgaaaatg aagaaatcaa aaatatctat
     2281 acagctttag gagttagaat cggtacagaa gaagattcca aagctttgaa tatggaaaaa
     2341 ttgcgttacc acaagttgat catcatgacc gatgccgacg tggatggtag ccacattgaa
     2401 acattgattc ttacattctt gttccgttac atgaaaccgt tgattgaatt gggttacgtg
     2461 tacgttgcaa cacctccttt gtaccaagtg aaaaaaggaa acaaatccga ttatgcttgg
     2521 aacgacgacc aacgcgattt gttgattcaa cagttcaaag gtgctggtgc tgattcttct
     2581 gttaacgtgc aacgctacaa aggtttgggt gagatgaacg cagaacaatt gtgggaaaca
     2641 accatgaatc cagaaggaag aacattgcgt caaattacta ttgaaaatgc tgccgaagca
     2701 gatcaaattt tctcaatgtt gatgggcgac gatgttccac ctcgtagaga atttattgag
     2761 aaaaatgcaa aatatgctaa aattgacgca taagacctca ttttaataga aaaaagcctg
     2821 tttttataat gaaaacaggc ttttttaatg cgatttatgt aaaaatcacg tatttttatt
     2881 gttagaataa aagttatgaa aatcatgaaa aaaattggtg ttttaacttc tggtggcgat
     2941 gctcctggaa tgaacgcttg catacgtgcc atcacaaaat tttgtttgaa tcacaaaatt
     3001 gaatgtgtag gaattatgga tggatacgac ggattaattc accaaaaagc aatcaatttg
     3061 ggatacgatg aagtcaataa catcattcaa cggggtggaa ccatccttgg gtcagccaga
     3121 agtaaagaat ttcaaaccgt tgaaggaaga aaaaaagcgc atgcattcat tactcaactc
     3181 caaattgatg gtcttattat tattggtgga gatggttcat tgactggagc aaaaattttt
     3241 agcgatgaat ttaactttcc aattattgga attcctggaa cgattgacaa cgacatactt
     3301 ggcagtgacc ataccattgg ctacgacaca gctgtaaaca cagttataga agctgttgac
     3361 aaaattcgcg atacggcaag tagtcataaa cgcatctttt ttgttgaagt gatggggcgc
     3421 gattctggat tgattgcctt aaattctgcc attgcatcgg gtgccgaatc tgttttaatt
     3481 ccagaaaaaa ataccgatat tgaaaaatta gtagaagaca tcaaagaagt gcacaaagga
     3541 aaacgcagcg ccatcatcat tgtggctgag ggcgatgacg ccggtgatgc tgtaaccatt
     3601 atgaaaaaag taaaaccact tctgtccgat tacgatttaa gagctaccgt tttgggacac
     3661 attcaacgag gtggtaaccc aacggcttac gacagaatca ttgcaactcg tatgggaatc
     3721 aaagccgttg aactgttgtt agaaaacaaa tcaaaattga tgatagcaac cgaaggcagt
     3781 aaattggtaa cccgttccat tgattttgca gaaaaaaaat cacccgacac ggcattgaca
     3841 aaacttcaaa ttattgaaca attaagaacc aaataaaatg caacaactca taaccaatat
     3901 tcaagaattg cccgcatttg ctacccaatt tttagataaa tatagccatc accgcatttt
     3961 tctttttgaa gcagaaatgg gatgcggaaa aacaacgttt attcaagaat tgttacgtca
     4021 aatgggagtt gaaaatccca acggttcgcc aacctactcc attgttaatg aatatacaac
     4081 gccaaactat ggtaaaattt atcattttga tttgtaccgc attcaagatg aagaagaaat
     4141 atacgacatt ggtatagaag acatgttata t
//
LOCUS       DAOQVQ010000172         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23966_length_4161_cov_3.492207, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000172 DAOQVQ010000000
VERSION     DAOQVQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23966_length_4161_cov_3.492207"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..733
                     /locus_tag="PKN22_06280"
     CDS             131..733
                     /locus_tag="PKN22_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42348.1"
                     /translation="MKWIGERISVVDDKNKTTIVIYPEKRALITGLMGAWIFMWYAIG
                     ITIVWSLFVLKLTDNEKVMLFVFMSFWAYYAVRVTRAFLWLMWGSENIKINEIGVTVK
                     NSIKKAGRAKIYYLENIKKARLEIPKQRSIQNAWESSPWINGGERIAFDYQGKVKRLG
                     RKLSEKDATLLFNLITKRIEEHLKNKTKKEKAAERQFQQD"
     gene            complement(730..1269)
                     /locus_tag="PKN22_06285"
     CDS             complement(730..1269)
                     /locus_tag="PKN22_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42349.1"
                     /translation="MLGTLLKKKLSDNQVANIFINALFETIDNGFPEVAQLINEDSAF
                     VSSPNIDANNNGEFALITIIGNLSFLESTFEPEQAARIEALIIDKLATIYQTTPSEFK
                     KIIADYKTFIQRVNHPSKNMIYGMSKAIFHKYQLNDFQDEYFKRMQAPNPLFLKRMDE
                     VVASFVWDWDAFFKKYRLN"
     gene            complement(1464..2246)
                     /locus_tag="PKN22_06290"
     CDS             complement(1464..2246)
                     /locus_tag="PKN22_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNS42350.1"
                     /translation="MITIFVAKLDFGVSENELFELFKQYGTVAKVTVAKDKETNKSRG
                     FAFVEMPNRDQAQQAIDALDGYSFNGRKCAVKEAENRSNTSSEQRGGGQANRFENRGP
                     RPENRGGRDENRGNRDEKKFFKPRTDDNRPKEAASIRVNDDNDDEPIIGKAEFTISDK
                     PSTAKKKKEDKPKTLDNTADGKNKKQKMDAYKKSGKDNIYFEDEDEEDLDLFGRNEEE
                     EEEEDYRKYLVNQDDEDEEWDEDEDEDWDEDDEDWDDEDDEY"
     gene            complement(2380..2769)
                     /gene="mscL"
                     /locus_tag="PKN22_06295"
     CDS             complement(2380..2769)
                     /gene="mscL"
                     /locus_tag="PKN22_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNS42351.1"
                     /translation="MLKEFRDFILRGNVIDLAVAVVIGAAFNAIVSSMVADIITPLLL
                     NPIMKASGAENLNSLGWNGVLYGKFIATVINFVVVGFVLFMVIKAMNKLQNLKKKKEA
                     EVTEEAPAPPTKDQELLTEIRDLLKKQ"
     gene            complement(2823..3434)
                     /gene="recR"
                     /locus_tag="PKN22_06300"
     CDS             complement(2823..3434)
                     /gene="recR"
                     /locus_tag="PKN22_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNS42352.1"
                     /translation="MNIPSKHLENVVEQLASLPGIGKRTALRLALHLFNRSKDEIDTF
                     AHSFIEMKEYVQKCNSCGNITDTPTCIICSNPKRDHSLICVVEDIRDILAIEATESFK
                     GIYHVLGAIISPMDGIGPNDLNIDSLIEKAKSGEVTEIIFALSATMEGDTTNFYLYKK
                     LKDFPISITTLSRGVSIGSELQYADELTLSRSITNRQPFKIQQ"
     gene            complement(3448..>4161)
                     /locus_tag="PKN22_06305"
     CDS             complement(3448..>4161)
                     /locus_tag="PKN22_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNS42353.1"
                     /translation="ILMDIQTEYARITTLIQKINGNHTESLRIAIIPTISNYLIADLF
                     ELVRKEYPHLQLTIEELKSEDLVEAMDNGLFDIGIMAGPQTSRRMRTVHLYQEEIFIY
                     FPQSTKNKITPEDLSNVQPWLLSEGNCLRTQMVNFCSIDKNSEHQWNYSGGNIDILTK
                     MVDLNGGYTLIPEYYNHQLKGIKRLQDTHTKEFPARQIIALTPHKSSKKDSIDALLRL
                     IQGNYNTQKKDDLRILDWK"
BASE COUNT         1246 a          712 c          737 g         1466 t
ORIGIN      
        1 agatttcacc tgaattaaag gggggcgaac acaaagttgt tttagtagta aaagatgctt
       61 gtggcaatat ttctacttgg gaaagaatga taacgtttca ataaactgaa tttttcacta
      121 atttcgcacc atgaaatgga taggcgaaag aataagtgta gtagatgaca aaaacaaaac
      181 aactattgtt atctatcccg aaaaacgagc gttaataaca ggattgatgg gcgcatggat
      241 ttttatgtgg tatgccatcg gaatcaccat tgtgtggtcg ttatttgttt taaagttaac
      301 tgacaacgaa aaagtaatgt tgtttgtttt tatgtctttt tgggcttact atgctgtgcg
      361 agtaacccgt gcattcttat ggttgatgtg gggttctgaa aatattaaaa tcaacgaaat
      421 tggagtaacg gttaaaaact caataaagaa agcaggtaga gccaaaattt attacttaga
      481 aaatattaaa aaggcacgct tagaaattcc aaaacaacgt tccattcaaa atgcgtggga
      541 atcctctccg tggattaatg ggggagagcg aattgctttt gattatcaag gaaaagtaaa
      601 acgtttggga cgaaaattgt ctgaaaaaga cgcaactttg ctattcaatt tgataacaaa
      661 acgcatagaa gagcatttaa aaaataaaac caagaaagaa aaagccgcag agcgacaatt
      721 tcagcaagat taattcaaac ggtatttttt aaagaaggca tcccaatccc atacaaaact
      781 agcaacaacc tcgtccatac gttttaaaaa caatgggttt ggtgcttgca tgcgcttaaa
      841 atattcgtct tggaaatcat tcaattggta tttatggaaa atagctttcg acataccata
      901 aatcatattt tttgatggat gattgacacg ttgaatgaag gttttataat cagcaataat
      961 ctttttgaac tcgcttggag ttgtttgata gatggttgct aatttatcga taatcaacgc
     1021 ttcaatacga gcagcttgtt cgggttcaaa agtactttcc aaaaatgaaa ggtttccaat
     1081 aatggtgatt agtgcaaatt ctccattgtt gtttgcatca atattgggtg atgaaacaaa
     1141 cgctgaatct tcattgatga gttgcgctac ttctggaaaa ccattgtcaa tcgtttcaaa
     1201 caacgcattg atgaaaatgt tggcaacttg attatccgat aatttctttt ttagcaaagt
     1261 ccctagcatg aacaaatgtt ttgtgttatt acaaatttaa gtagattgtt atatgtttaa
     1321 taagtagttg ttttgaagtt tattaacgaa gttattaaca aaaatgttga tagcttaaaa
     1381 aaagcagttt attaattttc agtgatttat ctttttaagc tcatgttagt aaaataaaaa
     1441 aagcaccaat ttggtgcttt ctcttaatat tcatcatctt catcatccca atcttcgtcg
     1501 tcttcgtccc agtcttcatc ttcatcttcg tcccactctt cgtcttcgtc gtcttggttg
     1561 actaagtatt ttctgtaatc ttcttcctct tcctcttctt cgtttctgcc aaacaagtct
     1621 aaatcttcct cgtcttcatc ctcgaaataa atattgtctt ttccgctttt tttgtaagcg
     1681 tccatttttt gctttttgtt tttaccgtcg gcagtattat ccaacgtttt tggcttgtct
     1741 tcttttttct ttttagcagt tgatggcttg tcagaaatag taaattcagc tttaccaatt
     1801 ataggttcat cgtcattatc atcgttaact ctaatggatg cggcttcttt aggtctatta
     1861 tcatcggtgc gtggtttgaa aaatttcttt tcatcacgat tgccacggtt ttcatcgcgc
     1921 ccgcctctat tttctggtct tggtccacga ttttcaaaac gattagcttg tccgccacca
     1981 cgttgttcag atgaagtgtt agatctgttt tctgcttctt taactgcaca ttttctacca
     2041 ttgaaagaat agccatctaa ggcatcaata gcttgttggg cttggtctct atttggcatt
     2101 tcaacaaagg caaaacctct tgatttgtta gtttccttgt cttttgctac tgttactttt
     2161 gctactgttc cgtattgttt aaatagctca aataactcat tttcagaaac gccaaagtct
     2221 agtttggcaa caaaaattgt aatcattatt tacgttaaac acttaattta tgggagctaa
     2281 gataaaatat ttcttttttc ttgcaaaata attttttaat aaaaaaaaat agttgccaaa
     2341 tcaattgaca actacttgtt ttataataag tttatgatct tattgttttt ttaataaatc
     2401 gcgaatttca gtcaataatt cttgatcttt tgttggtgga gctggtgctt cttcagtaac
     2461 ttctgcttct ttcttctttt tcaaattttg tagtttgttc atggctttga ttaccataaa
     2521 caatacaaaa cctacaacaa caaagtttat aactgttgca atgaatttac catataaaac
     2581 accgttccaa cctaaactgt ttaagttttc ggcacccgat gctttcataa ttggatttaa
     2641 taataatggt gtaataatgt ctgcaaccat tgaagaaaca attgcgttga aagctgctcc
     2701 aattacaact gcaactgcta aatcaattac gtttccacgt aaaataaaat ctctaaattc
     2761 ttttaacata ctactaattt taaggtttga ttatggacaa atatagataa aaaataaaac
     2821 tattattgct gaattttaaa aggttgtcgg ttggtgatgg atctactcaa ggtcaattca
     2881 tcggcatatt gcaactccga accgatagaa acaccacggg aaagcgtggt aatggaaatc
     2941 ggaaaatctt ttaatttttt gtataaataa aaattggtag tatcaccttc catggttgcg
     3001 ctcaatgcaa aaataatttc tgtaacttct ccagattttg ctttttctat aagtgaatca
     3061 atgttgaggt cgttgggacc aatgccgtcc atcggtgaaa tgatagcacc caaaacatga
     3121 taaattcctt tgaacgactc cgtagcttct atggccagaa tatcgcgaat atcctctaca
     3181 acgcaaatta atgaatgatc gcgtttaggg ttagagcaaa taatgcacgt gggagtatcg
     3241 gtaatattgc cacatgaatt gcatttttga acgtattctt tcatttctat aaatgaatgg
     3301 gcaaacgtat caatttcgtc ttttgagcgg ttaaataaat gcaaggctaa acgcaaagct
     3361 gttcgttttc caatgcctgg aagcgaagcc aattgttcaa ccacgttttc taaatgtttg
     3421 gatggaatat tcacggtgca aatgtattta tttccaatct aaaatgcgca aatcgtcttt
     3481 cttttgtgtg ttgtaatttc cttgaatgag tcgcaacaac gcatcaatac tatctttttt
     3541 tgacgattta tgtggggtga gtgctatgat ttggcgtgct ggaaattctt tggtgtgtgt
     3601 gtcttgcaag cgttttattc cctttaattg atggttgtaa tattctggaa taagcgtgta
     3661 gccaccgttt aaatccacca ttttggtaag aatatcaatg ttgcctccac tgtaattcca
     3721 ttggtgttca ctgtttttat cgatgctgca aaaatttacc atttgagtgc gtaagcaatt
     3781 accttcactc aacagccaag gttgaacatt tgataaatct tcgggggtaa ttttattttt
     3841 tgtgctttgt ggaaaataaa tgaaaatttc ttcttgatac aagtgtactg ttcgcatgcg
     3901 gcgactagtt tgtggacccg ccattatgcc tatgtcgaac aatccattgt ccattgcttc
     3961 cactaaatcc tcggatttta attcttcgat ggtaagttgc aagtgcggat attcttttcg
     4021 taccaattca aacaaatcag caatgaggta attactaatg gttggaatga tagcaatgcg
     4081 caacgattct gtatgattgc cattgatttt ttgaatcagc gttgtgatgc gtgcatattc
     4141 tgtttgaata tccattaata t
//
LOCUS       DAOQVQ010000173         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_23970_length_4160_cov_3.793423, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000173 DAOQVQ010000000
VERSION     DAOQVQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23970_length_4160_cov_3.793423"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4160)
                     /locus_tag="PKN22_06310"
     CDS             complement(<1..>4160)
                     /locus_tag="PKN22_06310"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42354.1"
                     /translation="PVAITYLPEITQTTTQDCQAGTVSTTVQGGAPAINGSNFTASNL
                     QPATASFTTSTVGNNGTIVVSGLQDGDNYSFDITDANGCPITVTGTFTGVTPSGFTYP
                     QTAYCKDAANPTPTITGATGGTFSGTAGLVINATTGVINIASTPAGTYTVTYASPGAP
                     CNSTSTFVVTINPLPTITVPSVAICIGSTATLNASGADTYTWSPATGLSATTGASVTS
                     TVTATQTYTITGTVTATGCTNTGTTTVTVNPLPTVNAGADVAICSGASTTLTASGAST
                     YSWDNGLGAGASHTVSPTVTTTYTVTGTNANGCVNTDQVTVTVNPLPTVTAGADVAIC
                     IGASTTLTAGGASSYSWSNGLGSGAIKTVSPTVTTTYTVTGTDGNGCVNTDQVTVTVN
                     PLPTISAGPDVAICAGSSTNLTASGGSTYTWDNGLGAGASHTVSPAATTTYSVTGTDA
                     NGCVNTDQVVVTVNPNPTPVISGSTEYCVGSVATVQTTQPYASYSWSNGQITPSINVT
                     QADNPIQVTVTTAAGCSGTSATFTVTEQSVITTTGTIEICQGQSAVIHGVTQTVAGVY
                     SNTQPSANGCDSTSNITLVVHALPTINAGADIAVCTGISVTLNATGAPTIVWSPTQTN
                     GIPFVQPVGSVTYTATGTDIHGCVNTDQVIVTVNPLPTVSAGTDVAICIGASTTLGAF
                     GATSYTWDNGLGAGANPTAAPTSTTAYTVTGTDANGCVNTDQVTVTVNPLPAVNAGPD
                     VAICIGASTILNATGAATYSWSHGLGTSATPSASPTTTTTYTVTGTDANGCVNTDQVT
                     VTVNPLPLVNAGVDVAICDGASTNLLASGANTYSWDNGLGAGAAKTVNPTTTTTYTVT
                     GTDGNNCVNTDQVVVTVNPLPTMAQQPNLTYCNGVTTNVITLNSNIAGSTFQWTNNNT
                     AIGLTSNGSGNILAFNATNTTSSAITATISVTPTSPLGCVGAPMSFTITVMPSPTATI
                     ANSAQVCINGTSPVVTFTGGTGTAPYTISYNINGGATQTITTTGNTATLNAPTNVAGI
                     FNYNLVSVQESGQSCTATITGNIAITVYDLPVISAGNDAAICIGSSTTITASGAGMGG
                     SYTWDNGLGVGASKTVNPTVTTTYTVTGTDNHGCQNTDAVVVTVNTPNGVDAGPNQTI
                     CIGENVTLNASSSDAASTFVWNNNVQNGVAFAPNQSTTYTVTATDGNGCISSDVMDVI
                     VNSLPIIEAGNNISGCAGDQFTFTASGAGQGGTYVWSNGIQNGVPYTPQAGDQQFYVT
                     GTDANGCVGYDSLFAKIQAFPVISFYADQEQNCAPVSATFYNTTQYPDAFSCMWYFDD
                     GTTYNGCGPVSHVFTSPGTYGATLVVTTDLAGCQSELYLPNLVVADSRPLAHFTPNPQ
                     IGTQINNQ"
BASE COUNT         1173 a          835 c          830 g         1322 t
ORIGIN      
        1 ttggttatta atttgtgtac caatttgtgg gtttggtgta aagtgagcta atggacgtga
       61 atctgcaact actaagtttg gcaaatacaa ttcactttga caacctgcta aatcagttgt
      121 taccactaac gttgcaccgt aagttccagg tgatgtaaat acgtgtgaaa ctggtccgca
      181 accattataa gttgttccat catcaaagta ccacatacat gagaatgcat ctggatattg
      241 tgtagtatta tagaatgtag cagatacagg agcacaattt tgttcttgat cagcatagaa
      301 tgaaatcact gggaatgctt ggattttagc aaataaagag tcatatccta cacatccatt
      361 ggcatctgtt ccagttacat aaaactgttg atctccagct tgcggtgtgt atggcactcc
      421 attttgaatt ccattgctcc aaacataagt tcctccttgt ccagcacctg atgccgtaaa
      481 ggtaaattga tcacctgcac aaccagaaat attgtttcca gcctcaataa ttggtaaact
      541 atttacgata acatccatca catcacttga aatacatccg ttaccatctg ttgctgtaac
      601 agtatatgtt gtagattgat taggtgcaaa agctactcca ttttgaacat tgttattcca
      661 aacaaacgtt gaagcagcat ctgatgaaga agcattcaag gttacgtttt ctccaataca
      721 aattgtttga tttggacctg catctactcc atttggtgta tttacagtta ccacaactgc
      781 atctgtattt tgacatccat gattatcagt tccagttacc gtataagttg ttgttacagt
      841 tggattcaca gttttagatg caccaacacc taatccatta tcccaagtat aagagccacc
      901 cattccagca ccagaggcag ttattgttgt tgaactacca atacaaattg ctgcatcgtt
      961 gcctgcacta attactggca agtcgtaaac tgttattgca atattacctg ttatagtagc
     1021 ggtacaggat tgtcctgatt cttgtacact caccaagttg tagttaaata tccctgcaac
     1081 attggtaggt gcattcaatg tagctgtgtt accagttgtt gtgatagttt gagttgcacc
     1141 accgttaata ttgtaggaaa ttgtataagg tgctgttcct gtaccaccag taaatgtaac
     1201 aactggagaa gtaccattaa tacaaacttg tgcactattt gcaattgttg cagttggcga
     1261 tggcattaca gtaattgtaa aactcattgg cgcccccaca cagcctagag gtgatgttgg
     1321 agtaaccgaa attgttgctg taattgcact agaagttgta ttggtagcat tgaaagctaa
     1381 aatatttcca ctaccattcg atgttaatcc aattgctgtg ttgttattgg tccactggaa
     1441 tgttgaaccc gcaatattgc tattcaaggt aataacattc gttgtaacgc cattacaata
     1501 tgttaaatta ggctgttgtg ccattgttgg caatggatta actgtaacaa ctacttgatc
     1561 agtattaaca cagttattac catctgtacc agttactgta tatgttgtcg ttgttgttgg
     1621 attcaccgtt tttgctgcac cagcacccaa tccattatcc caagaatatg tatttgctcc
     1681 agacgctaac aaattggttg aagctccatc acaaattgcc acatctaccc ctgcatttac
     1741 caatgggagt ggatttactg taacggttac ttgatctgta ttcacacaac cattagcatc
     1801 tgtaccagtt acggtatatg ttgttgtagt tgttggtgaa gctgaaggtg ttgcacttgt
     1861 tcctaatcca tgactccatg aataagtagc agcgcctgtt gcatttaaaa ttgttgaagc
     1921 tccaatacaa attgccacat ctggtccagc atttactgca ggaagtggat tcactgttac
     1981 tgttacttga tcggtattta cacatccatt agcatctgtt cctgtaacgg tatatgctgt
     2041 tgttgatgtt ggtgctgcag ttggattagc tccagcgcct agaccattgt cccaagtata
     2101 agaggtagct ccaaaggctc ctaatgtcgt tgaagcccca atacaaattg ctacatctgt
     2161 tccagcactt accgttggaa gtgggttcac tgttactatt acttgatcgg tatttacaca
     2221 cccatgaata tcagtaccag ttgcagtata agtaactgag cctacaggtt gcacaaaagg
     2281 aattccgtta gtttgcgttg gactccaaac aattgttgga gcacccgtag cattcaaagt
     2341 tacagagatt ccagtacata cagcaatatc tgctcctgca ttaatagttg gcaatgcatg
     2401 aaccaccaaa gttatgtttg aagtactatc acaaccattt gcagaaggtt gtgtatttga
     2461 atagacacct gctactgttt gcgttactcc atgaataaca gcactttggc cttgacaaat
     2521 ttctatagtt cctgttgtag tgattacaga ttgctcagtt acagtaaatg tggcagatgt
     2581 tccagaacaa ccagcagcag ttgtaactgt tacttgaatt gggttatcag cttgcgttac
     2641 gttaattgaa ggagtaattt gtccattcga ccagctataa cttgcataag gttgagtagt
     2701 ttgtactgtt gcaactgaac ctacacaata ttctgtacta cctgaaataa caggcgtagg
     2761 attaggatta acggttacta ctacttggtc tgtattcaca caaccattgg catctgttcc
     2821 tgttaccgaa taagtggttg ttgctgctgg agaaacagta tgacttgctc ctgcccctaa
     2881 accattgtcc catgtataag tggaaccacc tgatgctgtt aaatttgttg atgagcctgc
     2941 acatattgcc acgtctggac cagcagaaat tgttggtaat ggattgacag taactgtaac
     3001 ttggtcggta tttacacatc catttccgtc tgttccagtt acagtataag ttgttgttac
     3061 tgttggcgaa actgttttta ttgcccctga acccaaacca ttactccatg aataagatga
     3121 agcaccacct gcagttaaag ttgttgaagc accaatacaa attgctacat cagcacctgc
     3181 agtgacagtt ggtaatggat ttacggttac tgtaacttga tcggtattaa cacatccgtt
     3241 agcatttgtt ccagttacag tatatgttgt tgttactgtt ggagaaacag tgtgactagc
     3301 tccagctccc aacccattat cccatgaata agttgaagca ccgcttgctg ttaaggttgt
     3361 tgaagcacca ctacaaattg ccacatcagc acctgcgttt acagttggca aaggatttac
     3421 cgttacggtt gttgtccctg tatttgtaca tccagttgct gtaacagttc ctgttattgt
     3481 ataagtttga gttgcagtta ctgtagaagt aacagaagca cctgttgttg cacttaaacc
     3541 agttgcaggc gaccatgtat aagtatctgc acctgatgca ttcaaagttg cagttgaacc
     3601 aatacaaata gcaacactag gaactgtaat ggttggcaat ggattaattg ttactacaaa
     3661 tgttgaagtt gagttacatg gtgcacctgg agatgcgtat gttaccgtgt aagtcccagc
     3721 tggagttgaa gcaatattga ttactcctgt agttgcatta atcaccaaac cagcagtacc
     3781 tgagaaagtt cctcctgtag ctccagttat tgttggtgtt gggttagcag catctttaca
     3841 ataagcagtt tgtggatacg taaacccaga aggtgttaca cctgtaaaag ttcctgttac
     3901 tgtaattgga caaccattag catctgtaat atcaaacgaa taattatcac catcttgtaa
     3961 accagaaaca acgatagttc cattgtttcc tactgtactt gtagtgaaac tagcagttgc
     4021 tggttgcaaa ttggatgctg taaagttaga accatttatt gcaggtgctc caccttgcac
     4081 agttgttgaa acagtacccg cttgacaatc ttgcgttgtt gtttgtgtaa tttctggtaa
     4141 ataagtgatt gctacaggtg
//
LOCUS       DAOQVQ010000174         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_24146_length_4137_cov_3.358403, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000174 DAOQVQ010000000
VERSION     DAOQVQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24146_length_4137_cov_3.358403"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="PKN22_06315"
     CDS             <1..2135
                     /locus_tag="PKN22_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42355.1"
                     /translation="CGSQASYYHIGECYLGLKNNLSARAAFQKASEIASDPQLQEDAL
                     YQFAVLSYKLDVNPYNEAIRAFETYLNNYPNSKRKEDIYQYLVNVYTSTNNYQKAIES
                     LDRVNNKDITLQKAYQLIAYNYGVELSQKEQYQAAVDAFELVKKYPINPQLSAKAVFW
                     TGDANYELKKYGPSLANYRNYLGMIGANSEEMKSDAYYNMGYANLYLGDTVSAIENFR
                     TYEQRKPMNKQKLGDVYMRLADSYFMRRENQIAIKYYDLAIQSNVSNQDQALYYKALT
                     LGVENDKNGKIKALLDIVNNYPKSKYLQDALYEIAFSYKLLPDYDKALKYFNQLLTDY
                     PNPKKDADIHIEIADIYYKQNNYKKSEQEYLLVLEKYNYESVCGAVGDGLQNVFAATK
                     QVAKLEEYAKKYPCLNINAMTLENLVYNPAENLYTAKNYSEAIPKFEEYLAKYPQGYH
                     RKKAVYYLADAYYEIDSTAKSIALYEQILTEPTSNYTELAAIRAARYFYNNGNYAKAI
                     PYYERTVQATSDPEVIFNAETGLMRSYFQTKDYVPAAASAKNVLKSGLLKTENKLEAQ
                     YVLGVSCYQNKEYADALTALSFVSRNTTKAIASEAQFLIASCYFEQDKLKDADKQAQQ
                     VLKMKPTYDYWIAKALILQTKIQMKNNDLFQAEQTILSVIQQYPEENDGIKQEAQVVY
                     DELMQIKNMPKSTVVPTEATEVEINGGK"
     gene            2141..3841
                     /locus_tag="PKN22_06320"
     CDS             2141..3841
                     /locus_tag="PKN22_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42356.1"
                     /translation="MKKLVTLLLIGGSCTAFGQDVILQGQTRREIEKAQRIAARPVFD
                     DTVVQQKVVDYPLLVTQYKTQTEVDRIEPASIKIKEQLDQLYTTYVKLGFGTQLMPLG
                     EVYFNNRRSRKFIYGANVKHLSAWSNIPKYERNTFDRTSVNLYGGINEKRYQLFGDMH
                     YRNQGLHYYAIQAPKDSLGKANTRQRYNDFGFQLNYNGKAKIDTLGLDYRVGGQYNYF
                     NTLKMPVDSVTKWNASENFFALNGGVDYRLKGNVISVELGVKHNGYKYGNDSISPIDT
                     AITRSNTIINLYPSYNLFLLNNRFKASMGVDLTVDVDKKTKFYAYPMIEVSYSLFNNM
                     FIPYVGVRGNLKQNTLRSLTLENEFLRPNLAMSNENTVIDVYGGFKGSFSKSVTFNIG
                     GGYAMVKNLALFVNDTTYSPGNRFNVVYDNSKIFTLEGSISYQLLEKWKVDVIARYRS
                     YEMKTFAKAWNKPNFELITRGSYNLFDKVYFNLDFKLELGRKALVYAPGDKVKEENLQ
                     YFKALDPIYDINLGVEYRYTKRMSVFVQFNNIAAQRYQRWMKAPVHSFQFLGGLTFRF
                     "
     assembly_gap    3903..3912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3988..>4137
                     /locus_tag="PKN22_06325"
     CDS             3988..>4137
                     /locus_tag="PKN22_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790618.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HNS42357.1"
                     /translation="MSVRNLEPKELWNHFADINAVPRPSKREERIVQYMVNFGNSLGL
                     ETKTDA"
BASE COUNT         1439 a          703 c          779 g         1206 t
ORIGIN      
        1 attgtgggtc gcaagcatcc tattaccata taggtgaatg ttacttagga ttgaaaaaca
       61 acttatcggc acgtgcggca tttcaaaaag cgtcagaaat tgctagcgac ccacaattgc
      121 aagaagatgc cttgtatcaa tttgctgttt tgtcgtataa attagatgtg aatccctaca
      181 acgaagctat tcgtgcattt gaaacgtatt tgaacaatta tcctaactca aaacgcaaag
      241 aggatattta ccagtatttg gtgaatgttt acacgtcaac caacaattat caaaaagcca
      301 ttgaaagttt ggatcgtgtc aataacaaag acattacctt gcaaaaagcc tatcagttga
      361 ttgcctataa ttatggtgtt gagttatcgc aaaaagaaca ataccaagcg gcggtagatg
      421 cttttgaact tgttaaaaaa taccctatca atcctcaatt gtctgcaaaa gctgtttttt
      481 ggacaggtga tgcaaattat gaattgaaaa aatacggtcc ttctttggca aactatcgaa
      541 attatttagg aatgataggc gccaattccg aagaaatgaa atccgatgcg tattacaaca
      601 tgggttatgc gaatttgtat ttgggtgata cggtttctgc aatagaaaac tttagaacct
      661 atgagcaacg caaaccgatg aataaacaaa aattgggcga tgtgtacatg cgtttagccg
      721 atagttactt tatgcgtaga gaaaatcaaa tagctattaa atactatgat ttggccattc
      781 aatcaaacgt atctaaccaa gaccaagcct tgtattacaa agcattaacc ttgggggtgg
      841 aaaacgataa aaatggaaaa atcaaagcct tgttggatat tgtgaacaat tatcccaaat
      901 caaaatattt gcaagatgcg ttgtatgaaa ttgcattctc ttacaaatta ttgccagatt
      961 atgataaagc gttgaaatat ttcaatcaat tgttaaccga ttatccaaat cctaaaaaag
     1021 atgcggatat tcatattgaa attgccgata tttattataa acaaaacaat tacaaaaaat
     1081 cggagcaaga atatttgttg gtgcttgaaa aatacaacta cgaatcggtt tgtggtgcag
     1141 taggagatgg tttgcaaaac gtttttgcgg caaccaaaca agtggcaaaa ttggaagagt
     1201 atgccaaaaa atatccgtgt ttgaacatca acgcgatgac tttggaaaac ttggtgtata
     1261 atccagcaga aaatctttat acagctaaaa attactccga agccattcct aaatttgaag
     1321 aatatttagc aaaatatcct caaggatacc accgtaaaaa agcggtttat tacttggccg
     1381 atgcttatta tgaaattgac agcaccgcaa aatcaattgc gttgtatgag caaattttaa
     1441 ccgaaccaac gtctaattat accgaattgg cagccattcg cgcagcaagg tatttttaca
     1501 acaatggcaa ttatgcaaaa gcaattccgt attacgaaag aacggtgcaa gcaaccagcg
     1561 acccagaagt tattttcaat gcagaaacag gcttaatgcg tagttatttc caaactaaag
     1621 attatgttcc ggcagctgca tcggctaaaa atgtgttgaa aagtggcttg ttgaaaacag
     1681 aaaataaatt agaagctcaa tatgttttag gagtttcttg ctaccaaaac aaagaatatg
     1741 cagatgcgtt aacagccctc tcatttgtaa gcagaaacac tacaaaagca attgcttctg
     1801 aagctcaatt cttaattgca agttgctatt ttgaacaaga taaattgaaa gatgccgaca
     1861 agcaagcaca acaagtgttg aaaatgaaac caacctatga ttattggatt gcaaaagcat
     1921 tgatcttgca aacaaaaatt caaatgaaaa acaacgattt gttccaagca gaacaaacaa
     1981 ttttgtcggt tattcaacaa tatccagaag aaaatgatgg tatcaaacaa gaggcgcaag
     2041 tagtttacga tgaattgatg caaattaaaa atatgcctaa aagtacagtt gttccaacag
     2101 aagcaacaga agtagaaatt aacggaggaa agtaatgagt atgaaaaagt tagtaacctt
     2161 attattgatt ggcggtagtt gcactgcttt tggtcaagat gttattttac aaggtcaaac
     2221 acgtagagaa attgaaaaag cacaacgcat tgctgctcgt ccggtatttg atgatacggt
     2281 ggttcaacaa aaagtggttg attacccgtt gcttgtaacg caatacaaaa cgcaaacaga
     2341 ggtagatcgc attgaaccag cttctataaa aatcaaagaa caattggatc aattgtatac
     2401 cacttatgtt aaattaggat tcggaacgca attgatgcct ttgggcgaag tgtattttaa
     2461 caaccgccgt tcgcgcaagt ttatttatgg tgccaacgta aaacacttga gtgcgtggag
     2521 caatattcca aaatacgaac gcaatacgtt tgaccgtact tctgttaatt tatacggtgg
     2581 catcaatgaa aaacgttacc aattgtttgg tgatatgcat taccgcaatc aaggcttgca
     2641 ttattatgcc attcaagcac caaaagattc gttgggaaaa gcaaatactc gccaacgtta
     2701 caacgatttt gggtttcaac tcaactacaa cggcaaagca aaaattgata ccttgggatt
     2761 agattatcgt gttggtggac aatacaatta tttcaatact ttaaaaatgc ctgttgattc
     2821 ggtgactaaa tggaatgcct ctgaaaattt ctttgcattg aacgggggag tggattaccg
     2881 tttgaaaggt aacgtaattt cagttgaact aggagtaaaa cacaatggct ataaatatgg
     2941 taacgattcc atcagcccaa ttgatacagc aatcacgcgc tccaacacca ttattaattt
     3001 gtatccaagc tacaatttgt ttttgctcaa caatcgtttt aaagcatcta tgggggtaga
     3061 tttaacggtg gatgtggaca agaaaacaaa attctatgct tatcctatga ttgaggtgag
     3121 ctattccttg ttcaacaaca tgtttattcc ctatgtaggt gtgcgcggaa acttgaaaca
     3181 aaatacattg cgtagcctaa cacttgaaaa cgaattttta cgccccaacc ttgccatgag
     3241 taatgaaaat acagtaattg atgtttacgg tggttttaaa ggaagtttta gtaaaagcgt
     3301 tactttcaac attggtggtg gatatgccat ggtgaaaaat ttagcacttt ttgtaaacga
     3361 cactacctat tctccgggca accgatttaa tgtggtgtac gataactcaa aaatcttcac
     3421 tttagaaggt tctatttcat accaattact tgaaaaatgg aaggtagatg ttattgccag
     3481 ataccgttcg tatgaaatga aaacatttgc caaagcatgg aacaaaccta attttgaatt
     3541 gataactcgc ggaagctaca atttgtttga taaagtatat ttcaacttgg attttaaatt
     3601 agaattgggc agaaaagcat tggtttatgc ccctggcgat aaagtaaaag aagaaaattt
     3661 acaatacttc aaagcattgg atccaattta cgacatcaat ttaggagtag aataccgtta
     3721 caccaaacgc atgtctgttt ttgttcaatt caataacatt gctgcacagc gataccaacg
     3781 ttggatgaag gctcctgtgc acagcttcca atttttgggt ggtttaacct ttagatttta
     3841 aatagtaatt taactagtaa attcaactcc gtagtggttt tctgctacgg agttttttat
     3901 gcnnnnnnnn nnatgcacta acttcttttc tgttgcattt cttttcattc tcaatcattt
     3961 cattaaattt gtgtaaaaat caaatttatg tcagtgcgta acttagaacc gaaagaattg
     4021 tggaaccatt ttgcagatat caacgctgtt cctcgtccat caaaaagaga agaaagaatt
     4081 gttcaataca tggtaaattt tggtaattca ttgggattgg aaacaaaaac cgatgcc
//
LOCUS       DAOQVQ010000175         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_24206_length_4131_cov_3.387390, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000175 DAOQVQ010000000
VERSION     DAOQVQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24206_length_4131_cov_3.387390"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKN22_06330"
     CDS             <1..231
                     /locus_tag="PKN22_06330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS42358.1"
                     /translation="ISSNTGGIPEVNEHGFSGFTSNVGEVQDMANNTLKILQSDETLT
                     QFKKNAKLQAQKFELDKILPMYEDLYQQVVNL"
     gene            360..713
                     /locus_tag="PKN22_06335"
     CDS             360..713
                     /locus_tag="PKN22_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42359.1"
                     /translation="MNKIKFLLFSLFIIFSSFISSEEVFQQFSNLDKFVATTGVENVD
                     QLQNTTDSPVEITIIKYRKLRRGNTSQNFSIQTEIESLNNEHSINSIAQLNFEVTTCS
                     LLPKETSFLHRYQLF"
     gene            777..1871
                     /locus_tag="PKN22_06340"
     CDS             777..1871
                     /locus_tag="PKN22_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS42360.1"
                     /translation="MNKVFYLAFVVSGLFFTACSDDNQQHNTNDIKTLPILKVALNDT
                     IVSNKFVADIQAKKNVEIHARIAGLMEKVFVNEGQAVKKGQTLFKLSDAELQIELLKA
                     KATLKNAQADFRMTSVELRQMKALFDKKIIADNELELAQAKNEASEAKVAYAMAEKNA
                     IEQQISFTKIVAPFDGVIDRIPLKEGSLVQNGSLLTTVSELDEIYAYFSIQENTYFQL
                     IAENKLGNHRDIHLVLPNGVKYAHQGVLETAEGEIDRQTGTIQYKAKFANTEGMIKHG
                     TSGKLIISESREDVIIIPQKSVFSIQDKRYVFVVDKNNVVHMTPVVIGATLEDSFIID
                     KGLKVGDVIVQAGTQSLRDGEKIKRKKSTK"
     gene            1894..>4131
                     /locus_tag="PKN22_06345"
     CDS             1894..>4131
                     /locus_tag="PKN22_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS42361.1"
                     /translation="MVELFIRRKVLSLVISIFFVLLGVLAIFQLPITQFPDIVPPSVI
                     VQAKYTGANAEVSANAVALPLERAINGVPGMTYMSTVTSNDGLTMIQIFFEVGTDPDL
                     AAVNVQNRVTTILDELPEEVIRAGVTTEKEVNSMLMYLNIVSTDEDQDEQFIFNFTDI
                     NILQELKRIDGVGRAEIMGQKEYSMRVWLDPEKMVAYNIAANEVIDALQKQNIAAAPG
                     KVGEGSGKIKSELQYVIKYTGKFFEPHQYEEIPLRSNPDGTILKLKDVAQIEFGAMSY
                     GMVSKTDGRPSASIMMKQRPGSNASEVIANVKAKMAELKETSFPPGMDYNLAYDVSRF
                     LDASISEVLKTLIEAFILVGIVVFLFLQDWRSTLIPVLAVPVALVGTFAFMSMLGFSI
                     NLLTLFAMVLAIGIVVDNAIVVVEAVHVKMVEQHMPPLEATISATKEIAGAVVAITIV
                     MAAVFIPVAFLDGPVGVFYRQFSLTLAISIVISGINALTLTPALCALILKPHNPTTKK
                     GWLGKFFDKFNAWFDRVTNGYANILSKFATRPVVTIGILLAFVGLTWGTASILPSGFI
                     PMEDQGMLYVNVTTPQGASVERTEKVLDEVAAMAKEIEGFENVTTLAGYNIVSEIAGA
                     SYGMGMINLKDWKDRSITVDEFIAQLQEKTEDIADASIEVFAPPTVPGFGNTSGFELR
                     LLDKSGGTIENTDEIAKKFVADLNASPEIKNTFTNFDATFPQYMIHIDYDMAAKKGVS
                     VDNAMS"
BASE COUNT         1364 a          683 c          810 g         1274 t
ORIGIN      
        1 atttcatcca atacgggtgg catcccagaa gtgaacgaac atggatttag tggttttact
       61 tccaatgtag gtgaagtgca agatatggct aacaacactt tgaaaatttt acaatcggac
      121 gaaactctaa ctcaatttaa gaaaaacgct aaactacaag cgcaaaaatt tgaattggat
      181 aaaattttac cgatgtatga agatttgtat cagcaagttg tcaatttata aggattttac
      241 aggcgattaa ataaaattac acagctttca attaacttat tttaacaaat cagtttttaa
      301 agaagctcac ttttgatgtt aattttgtaa actttttatg cagaaagcaa ccaaaaaaaa
      361 tgaataaaat taaattttta cttttttctc tttttatcat tttttcttca ttcatttcga
      421 gtgaagaggt gtttcagcaa ttttcaaatc tcgataaatt tgtagcaaca acaggagtcg
      481 aaaatgttga tcaattacaa aatacaacag attcaccagt agaaatcact atcataaaat
      541 acagaaaatt aagacgtggt aatacaagtc aaaatttctc tattcagact gaaattgaat
      601 cccttaacaa cgaacatagt atcaattcaa ttgctcaatt gaattttgaa gtaactactt
      661 gttcacttct tcccaaagaa acatcttttc tgcatcgtta tcaattgttt tgatttaaaa
      721 taagacttcc aaactacttc tgtagattaa ctagttttta ttttaaataa ttgataatga
      781 ataaagtatt ttaccttgca tttgttgtaa gcggtttatt tttcacagct tgtagtgatg
      841 ataatcagca acacaacaca aacgatatta aaacacttcc tatattgaaa gtggcattaa
      901 atgacaccat tgtttccaat aaatttgttg ccgatattca ggctaaaaaa aatgttgaaa
      961 tccatgcccg aattgccggt ttgatggaaa aagtatttgt aaacgaaggt caggcagtaa
     1021 aaaaaggaca aacacttttt aaattaagtg atgctgaatt gcaaatagaa ttgctcaaag
     1081 caaaagcaac attaaaaaat gcacaagctg attttcgtat gacttcggtt gaattacgtc
     1141 aaatgaaagc tttgtttgat aagaaaatta ttgccgataa tgaattagaa ttggcgcaag
     1201 caaaaaatga agcttcggaa gcaaaagttg cctatgctat ggcagaaaaa aatgccatcg
     1261 agcaacagat cagctttaca aaaattgtag ccccttttga tggtgtaatt gacagaattc
     1321 cattaaaaga aggaagtttg gtgcaaaacg gttcattgct tacaacagtt tctgagttgg
     1381 atgagattta tgcctatttc tctattcaag aaaatactta ttttcaatta attgctgaaa
     1441 ataaattagg aaatcaccgc gatattcact tggttcttcc aaacggtgta aagtatgcac
     1501 atcaaggtgt gttggaaaca gcagaaggtg aaattgaccg ccaaacaggt actattcaat
     1561 acaaagcaaa atttgccaac accgaaggaa tgataaaaca cggtacatcg gggaaattaa
     1621 ttatttctga aagtagagaa gatgtcatta ttattcctca aaaatcagta ttttccattc
     1681 aagataaacg ttatgttttt gtagttgata aaaataatgt tgttcacatg actccagtag
     1741 taattggcgc aaccttggaa gattctttca ttatcgataa aggattaaaa gtaggtgatg
     1801 ttattgttca agcaggaaca caatcactgc gcgatggaga aaaaatcaaa cgcaagaaaa
     1861 gtaccaagta gtttttctta aaaatcaagc tttatggtag aattatttat tcgacgaaaa
     1921 gtactttcgt tagtaatttc aatatttttt gttttgttgg gggtattggc tatttttcaa
     1981 ttgccgatta cgcaatttcc tgacattgtg cctccatccg ttattgtgca agccaaatat
     2041 actggtgcaa atgccgaggt gtcggccaat gcagttgcct tgccattaga acgtgctatc
     2101 aacggggtgc cgggaatgac ttatatgtca actgtaacat ccaacgatgg attgacaatg
     2161 attcaaattt tctttgaagt tggaacagat cccgatttgg ctgcggtaaa cgtacaaaac
     2221 cgcgtaacta caatcttgga tgagttgccc gaagaggtta ttcgtgctgg tgttactacc
     2281 gaaaaagagg tgaacagtat gttgatgtac cttaatattg tgagtacgga tgaagatcaa
     2341 gacgagcaat ttattttcaa tttcacagat atcaacattc ttcaagaatt aaaacgtatt
     2401 gatggagttg ggcgtgccga aatcatggga caaaaagaat attccatgcg tgtttggtta
     2461 gacccagaaa aaatggtggc ctacaacatt gctgcaaacg aagtaattga tgcattgcaa
     2521 aagcaaaaca ttgccgcagc accaggaaaa gtgggtgaag gctctggtaa aattaaaagt
     2581 gaattgcaat acgttatcaa gtacacagga aagttttttg aaccccacca atacgaagaa
     2641 attcctttac gttccaatcc tgatggaaca atattgaaat tgaaagatgt tgctcaaatt
     2701 gaatttggag ccatgagcta cggaatggtg tcaaaaacgg atggtcgccc ttctgcttcc
     2761 ataatgatga aacagcgccc tggttccaat gcttcggagg ttattgccaa tgtaaaagct
     2821 aaaatggccg agctaaaaga aacttctttt cctccaggaa tggactataa tttagcctat
     2881 gacgtttcgc gttttttgga tgcttccatt agcgaagttt taaaaacttt aattgaagcc
     2941 tttatcttgg ttggtatagt ggtattcttg tttttgcaag attggcgctc tacgttgatt
     3001 cctgtattgg cggttcctgt tgctttggtt ggaacgtttg catttatgtc catgctcggt
     3061 ttctctatca acttgttaac cttgtttgca atggtgttgg ctatcggtat tgtggtggat
     3121 aatgccattg tggtggtaga agccgtgcac gtaaaaatgg tagaacaaca catgccacct
     3181 ttagaagcta ccattagtgc aaccaaagaa attgcaggag cagttgtagc tattaccatc
     3241 gtaatggcgg ctgtgtttat tcctgttgcg tttttggatg gaccagtggg tgtgttttac
     3301 cgccaatttt cactaactct tgccatcagc attgttattt cgggtattaa cgcgttaaca
     3361 ttgacaccag ctttgtgtgc tttgatttta aaaccacaca atccaacaac caaaaaagga
     3421 tggctgggca aatttttcga taaattcaat gcttggtttg atcgtgtaac gaatggttat
     3481 gccaatattt tgtcaaaatt tgcaacacgt ccagttgtaa caattggaat tttattagca
     3541 tttgttggtt taacttgggg aacggcttcc attttacctt ctgggtttat tccaatggaa
     3601 gaccaaggta tgctgtatgt taacgttacc actccacaag gagcatcggt agaacgaact
     3661 gaaaaagtgt tggacgaagt tgccgctatg gcaaaagaaa ttgaagggtt tgaaaacgta
     3721 acaacattgg caggttataa cattgtttct gaaattgcag gtgcttctta cggaatggga
     3781 atgataaacc ttaaagattg gaaagacaga tcaattacgg tggatgaatt tattgcacaa
     3841 ctgcaagaaa aaactgaaga cattgccgat gcaagtatag aagtgtttgc accaccgaca
     3901 gttccaggtt ttggtaatac cagtggtttt gaattgcgcc tattggataa atcaggagga
     3961 acgattgaaa acaccgatga aattgcaaag aaatttgttg cagacctaaa tgcgtctcct
     4021 gaaatcaaaa atacgtttac caatttcgat gcaacctttc cacaatacat gattcatatt
     4081 gattatgaca tggcagccaa aaaaggtgtt tcggtggata atgcaatgtc a
//
LOCUS       DAOQVQ010000176         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_24276_length_4123_cov_4.588741, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000176 DAOQVQ010000000
VERSION     DAOQVQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24276_length_4123_cov_4.588741"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..2391)
                     /locus_tag="PKN22_06350"
     CDS             complement(172..2391)
                     /locus_tag="PKN22_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42362.1"
                     /translation="MKKNILFAFALLFSLVANAQINGIIVGSDNGKMEPLLNAKAFLV
                     HAKTGALSHEDGRFELILPRQLPDTLVFSYPGYFSDTLVVTKEDRFSNLKVVLFSDQT
                     LPEFIVEYRKEAKSISKLKVLAVEELSLGELRKAACCNLSESFETNVSVDVNFTDAVS
                     GAKQIQMMGLEGVYTQIQFENIPYLRGLEQPFGLSSFPGTWINSIQITKGTGTVVNGY
                     ESMAGLVNIEMFKPQDMDRFFINGYGGVYGRGELNLHGSMHVGKKEKWSTALFAHGSG
                     VFVENNYNKDNFMDMPMGYLAAFNNRWNYQGKKMEAQIGVNSYLESKYGGEVGYKRGS
                     NKGLFGMETDAKHLDIYAKTGFFMKKPYNSIGIIYNLKYQENDALFGLRRFSGTEKRG
                     YINGMFEGIMGTTIHKYKVGLSGVFADFKQRMDTLTDNRLEIVPGAFFEYTYTIPRFT
                     LVLGARGDYHNLYKFQFAPRMHAKFAITEYTDLRVTAGKGWRVPNYMIDNISLLATSH
                     RWVAPTETLPEISWNFGGSLVQSFKLFGNPASVVVDYYHTLFERQLIVDRDQDMTAIV
                     FKNMKGKSFSNSVQVEFSFQPAKRFDIRLAYKFLDVRAEYGGKLQQKVMVPKHRGFIN
                     LFYTTRNKKWEFNLTTSVFGEARLPVNQLDSLNFTTDNKSKVYPQLSAQVTYKHKRWD
                     FYIGGENLTNYRQKNPIIDADNPFGTYFDATRIWAPITGVNVYVGFRFVIEHKEHKD"
     gene            complement(2446..2922)
                     /locus_tag="PKN22_06355"
     CDS             complement(2446..2922)
                     /locus_tag="PKN22_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42363.1"
                     /translation="MFSKKYRHIVHRAVVALSLVAFLLANVGMLVYTHSCTISGTEKT
                     LFAASEDPCEEDIHLVKEPCCSEENTTNSQHSTKIDEGCCTNNADYVALNIDLHTDFH
                     PLHFALIPFDANAFPTPVWENVASDATVSYGEHYIKSPPDIYQGRDLQSIHQVYII"
     gene            complement(3027..3974)
                     /locus_tag="PKN22_06360"
     CDS             complement(3027..3974)
                     /locus_tag="PKN22_06360"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42364.1"
                     /translation="MKKIVLTFCILLIGFIGFSQKQTKYIGFYNIENLYDTINQANDD
                     EEFLPNGENKWTSERYLEKLKNLNQVIDSMGNMVLLGFAEVENKTVLQDLNKASKTRK
                     KYKIVHFDSPDKRGVDASMIYNPKFLKLVDSGKIGLVLEVGKSDTRDIVWAKFTHKKD
                     TLFAMVNHWPSRRGGEKESEPNRIKAATTAAVFIDSVMKASPASKIVFMGDLNDYPTN
                     VAPKMIAERLTPMISKASGKFGGSYNYKNEWDVLDHIMVSPNAFSGKLQVKENSGEIV
                     SNDFLLVEYKGNIVPKRNYAGSKYLKGYSDHLPVRIQLK"
BASE COUNT         1214 a          822 c          763 g         1324 t
ORIGIN      
        1 ttttgtgtag ttcaatttgt cttctaagcg gactttgcaa tcgttgcatt ctgccgaagt
       61 ttgaataact acttcttcta cttttttcaa ttcttgcgca aagctgtttc caataaaaag
      121 caatgcaaaa gcgactaata aatttttcat gttctgttta ttttaattgt tttaatcttt
      181 atgttctttg tgttcaatca caaaacggaa tcccacatac acgtttacac ccgtaattgg
      241 cgcccaaatt ctggtggcat caaaatatgt tccaaacgga ttatcggcat caataatagg
      301 atttttttgg cgataattgg tcaagttttc tccaccaatg taaaaatccc aacgtttgtg
      361 tttgtatgtt acttgtgcac tcaactgcgg atataccttg ctcttgttgt cggttgtaaa
      421 gttaagtgaa tctaactggt taactggcaa acgagcttct ccaaacacag atgttgtgag
      481 gttaaactcc cattttttgt tgcgggttgt ataaaacaaa ttgataaatc cgcggtgttt
      541 tggcaccatt actttttgtt gcaatttacc accatattct gctctaacgt ctaagaattt
      601 ataggctaaa cgaatgtcaa aacgtttggc tggttggaaa gaaaattcaa cttgcacgct
      661 gtttgaaaac gattttcctt tcatgttttt aaacacaata gccgtcatgt cttgatctct
      721 gtccacaatc aattggcgtt caaacaaggt gtggtaataa tcaaccacca ccgaagctgg
      781 gttgccaaat agtttgaacg attgcaccaa cgaaccacca aaattccacg aaatttcggg
      841 caatgtttca gttggagcca cccagcggtg cgatgttgcc aacaaggata tattgtcaat
      901 catgtagtta ggaaccctcc agccttttcc agcagttaca cgcaagtcgg tgtattctgt
      961 gatggcaaat ttggcgtgca tgcggggtgc aaattggaat ttatacaagt tgtggtaatc
     1021 gccacgtgca cccaaaacca acgtaaagcg tggaatggtg taggtgtatt caaaaaatgc
     1081 accgggcact atctccaaac ggttgtcggt tagggtatcc atgcgttgtt tgaaatcggc
     1141 aaaaacaccg ctcaatccta ctttgtattt atggatagtg gttcccataa ttccttcaaa
     1201 cattccgttg atgtaaccac gtttttcggt tccagaaaat cgacgtaagc caaaaagcgc
     1261 atcgttttct tggtatttta agttgtaaat tatcccgatg gagttgtaag gtttcttcat
     1321 aaaaaaccct gttttggcat aaatatccaa gtgtttggca tcggtttcca taccaaataa
     1381 acctttgttt gaaccacgtt tgtagccaac ttcgccccca tatttagatt ctaaataaga
     1441 gttaacccca atttgtgctt ccattttctt tccttggtaa ttccaacggt tgttaaacgc
     1501 tgctaagtaa cccatgggca tatccataaa attgtctttg ttgtagttgt tttctacaaa
     1561 aacacccgaa ccgtgggcaa acaaggcagt gctccatttt tcttttttac ctacgtgcat
     1621 acttccgtgg aggttcaatt cgcctctacc ataaacaccg ccataaccgt tgataaaaaa
     1681 acgatccatg tcttggggtt taaacatttc aatattcacc aaccctgcca tagattcgta
     1741 gccattgaca acagttcctg ttcccttggt aatttgaatg gagttgatcc acgtgcctgg
     1801 aaaagaggac aacccaaatg gttgctccaa tccacgcaaa tagggaatgt tttcaaactg
     1861 aatttgggta taaacacctt ccaatcccat catttgaatt tgtttggctc ccgaaacagc
     1921 atctgtaaag ttgacatcca ccgaaacatt ggtttcaaac gattctgaca agttacaaca
     1981 agctgctttg cgcaattcgc ccaacgatag ttcttcaact gccaacactt tgagtttgga
     2041 aatggatttt gcttctttgc ggtattccac aataaattcg ggcaatgttt gatcagaaaa
     2101 caacaccact tttaagttag aaaaacgatc ttctttggtt accaccaagg tgtcggagaa
     2161 ataacccgga taagaaaaca ccaaagtatc aggcaattga cgcggtagaa tcaattcaaa
     2221 acgaccatcc tcgtgcgata atgcacctgt tttggcatgt actaaaaatg cttttgcatt
     2281 caatagtggc tccattttgc cgttgtcgga gcctacaata attccgttaa tttgtgcatt
     2341 tgctacaagt gaaaacaata gcgcaaatgc gaataaaata ttttttttca ttgattattt
     2401 gattaaaatt caattaatta agttgcctta aaaatgaatt ttaaatcaaa tgatatagac
     2461 ttggtgtatg ctttgcaaat cgcgcccttg gtatatatcg ggtggcgatt tgatgtagtg
     2521 ttcaccataa gaaacagttg catctgaggc tacattttcc caaactggag taggaaatgc
     2581 gttggcatca aaaggaatga gtgcaaaatg tagcggatga aaatcggtgt gcaaatcaat
     2641 gttgagcgca acataatcgg cgttgttggt gcaacatcct tcgtcaattt tagttgaatg
     2701 ttgcgaattg gttgtgtttt cttccgaaca gcaaggttct tttaccaaat gtatatcttc
     2761 ttcgcatgga tcttcggatg cagcaaacaa ggttttttct gttcccgaaa tggtgcaaga
     2821 atgggtataa accaacattc caacattggc caacaaaaag gcaaccaacg aaagtgcaac
     2881 aactgcacgg tgaacgatat gtctatactt ttttgaaaac attaaggcaa aaataaagaa
     2941 actaacggct gaaagaataa aaaaatcgtt aaaaatgccg tttacacaaa atcatgtaac
     3001 aaaccagcaa aattttaaaa acggtattat ttcaactgaa tgcgaaccgg taaatggtcg
     3061 gaataccctt ttaaatactt ggatccggca tagtttcttt tcggaacaat gtttcctttg
     3121 tattcaacca acaaaaagtc gttggataca atctctcccg aattttcttt tacttgcaat
     3181 ttaccagaaa aagcatttgg cgaaaccatg atgtggtcta acacatccca ctcgtttttg
     3241 taattgtagg aaccaccaaa tttaccagaa gctttagaaa tcattggtgt taaacgttct
     3301 gcaatcattt ttggagcaac gttggttgga taatcgttca aatcacccat aaacacaatt
     3361 tttgaagcag gcgatgcttt catcaccgaa tcaataaaaa cggctgctgt agttgctgct
     3421 ttaatacggt tgggttcgct ttctttttca ccacctctac gcgaaggcca atgattgacc
     3481 attgcaaaca aggtgtcttt tttgtgtgta aattttgccc atacaatatc gcgggtgtct
     3541 gattttccta cctccaatac taatccaatt tttccgctgt ccaccaactt taaaaatttt
     3601 gggttgtaaa tcattgaagc atccacacca cgtttgtcgg ggctatcaaa atgtacgatt
     3661 ttgtattttt ttctggtttt agacgctttg tttaaatctt gcaataccgt tttattttcc
     3721 acttcggcaa agcccaacaa caccatgttg cccatggagt cgataacttg attcaaattt
     3781 tttagctttt ccaaatagcg ttcactcgtc catttgtttt cgccattggg caaaaattct
     3841 tcatcatcgt tggcttggtt gatggtatcg tataagtttt cgatgttata aaaaccgatg
     3901 tatttagttt gtttttgcga aaaacctata aatcctatca ataaaatgca aaaagtaagg
     3961 acaatttttt tcatagtttt aagtgtgtgt taaaattaat catcgcgtct gtccttgcgt
     4021 ttttcgggtg gagaaaacgg cagcggattg gcaaaaattt ctttgtgtaa tttattgttt
     4081 ttgtaaatat ccatagctaa ataaatggca cttctaagcg att
//
LOCUS       DAOQVQ010000177         4092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_24542_length_4092_cov_3.764677, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000177 DAOQVQ010000000
VERSION     DAOQVQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24542_length_4092_cov_3.764677"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..2367
                     /locus_tag="PKN22_06365"
     CDS             274..2367
                     /locus_tag="PKN22_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS42365.1"
                     /translation="MAEATAKTVKNKIVTDKETLLKAFELMATAKTLAEKYEANKEVT
                     SKYVHATSRGHEAIQLAVGMQLLPQDWVAPYYRDDSILLGIGMAPYDLMLQVFAKKDD
                     PFSGGRTYYSHPSLKRDDMPKIPHQSSATGMQAIPMTGVAMGIQYKEMQGLAEDFGGK
                     NPVVVCSLGDASCTEGEVSEAFQMAALKQFPIVYLVQDNGWDISATAAETRAQDITHY
                     AQGFNGIEVRTIDGTDFDLSFQTVKEVFEIVRKERRPFIIHAKVPLLGHHTSGVRKEW
                     YRNDLEEAAKRDPYPKIVAKLMELGVEETTLDTVENQVKTLVDTDYTRALNAEEPDTA
                     SVTDFIFAPSPITEEKGEREPKGKKKTVMVDSALFAVREILEKHPEALLYGQDVGGRL
                     GGVFREAATLAQQFGDNRVFNTPIQEAFIIGSTVGMSAAGLKPIVEVQFADYIWPGLN
                     QLFTEVSRSNYLSNGKWPVSCVIRVPIGAYGSGGPYHSSSVESVLTNIRGVKTVYPST
                     GADLKGLLKAAYYDPNPVVILEHKGLYWSKIKGTEGAMSVEPDEDYVIPLGKGRIVQH
                     ADEKVVKNGESCVVITYGRGVYWTLEACETFKDKVEIVDLRTLHPLDFELINEMAQKH
                     SKVLLVTEESVEASFTLGLAGRIQRDNFKALDAPVAIVGSVDTPAIPMNSSLEATLLP
                     NADKVKKALEDLLAY"
     gene            complement(2424..3032)
                     /locus_tag="PKN22_06370"
     CDS             complement(2424..3032)
                     /locus_tag="PKN22_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNS42366.1"
                     /translation="MKLIIESPNIDDYLAEIPPIITFNTPMISQEIEWIKEQTSNPEK
                     QARIAFEIARDDIFHSFDTKNPLVTINAEDVLKSSEGICFSKSHLLVALLRGLSIPAG
                     FCYQRVLRVPTDENSGFALHGLNAIYLREYGWFRVDPRGNKPGVNSQFSIEPEQLAYT
                     IHPHLGEVDYPTVYAKPLDSVIQSMQESATTQELFFNRPDSI"
     gene            3158..4009
                     /locus_tag="PKN22_06375"
     CDS             3158..4009
                     /locus_tag="PKN22_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNS42367.1"
                     /translation="MNKLLTTILAASVVSLASAQVKTPKASAHAELEATVGLTEIEVE
                     YSRPNKNNRVVFGDVVPFDKIWRTGANKNTTIEFSDDVTVGGAPLKAGKYAIYTKPGK
                     SAWEVYFYTDTENWGNPQTWDDAKVAAKAVAKVEQLSQPVETFTISVDDLKMDKANLT
                     FSWDKTRAVLPIVVPTDAKVMETIEKTMNAEPKAGDFIAAANYYASTGKDAQLAKDWM
                     DKGMSLMKEPQYYHYHQQAVVYANAGDFKGAVEIAKKSLEGAKAAGDDAYVKKNEVAI
                     KEWANKK"
BASE COUNT         1327 a          660 c          894 g         1211 t
ORIGIN      
        1 gtaggcaaac cttaaattcg tttatcaatg caaatttatg ggacgaagca cgtgttttaa
       61 ctggaaatgt ggaatggaaa gaaggtgtat ttgccccacg tttgccgcat caatattttg
      121 ctgaagaatt tcaactaggc caagataaag ttacaattta tcgccacttt aatagttaat
      181 attttaacgt tttactattt tttcaaacag attaaacatt ttgttttttt ttgtacatta
      241 ctttttgtaa ttttgaaggg taattattaa agaatggcag aagcaacagc aaaaaccgta
      301 aaaaataaaa ttgtaacaga caaagaaacc ttactcaaag catttgagtt aatggcaacc
      361 gcaaaaactt tggctgagaa atacgaagca aacaaagaag taacatctaa atatgtgcat
      421 gcaacatcta gaggccatga agcaattcaa ttggcagtgg ggatgcaatt gttaccgcaa
      481 gactgggtgg caccatatta ccgcgatgac agtattttgt tggggatagg gatggcgcct
      541 tatgatttga tgttgcaggt atttgcaaaa aaagacgatc ctttttctgg aggaagaact
      601 tattattcgc atccttcatt gaaacgcgat gatatgccaa aaattccaca tcaatcttct
      661 gcaacaggga tgcaagctat tcctatgaca ggggttgcga tgggaatcca atacaaagaa
      721 atgcaaggat tggctgaaga ttttggtgga aaaaatccag ttgttgtttg ttcattgggt
      781 gacgcttctt gtacggaagg cgaggtttct gaagcgttcc agatggctgc tttgaagcaa
      841 tttcctattg tttacttggt gcaagacaac ggttgggata tttctgctac ggcagctgaa
      901 acccgtgcac aagatatcac gcattatgca caaggattta acggcataga ggttcgcaca
      961 attgatggca ccgatttcga tttgtcattt caaacagtaa aagaagtgtt tgaaattgtg
     1021 cgtaaagagc gccgtccgtt tatcattcat gcaaaagttc cattgttggg tcaccacact
     1081 tctggtgttc gtaaagaatg gtaccgcaac gatttggaag aagctgcaaa acgcgatcct
     1141 tatccaaaaa ttgttgcaaa attaatggaa ttgggggtag aggaaactac cttggatacg
     1201 gttgaaaacc aagtaaaaac tttggttgac accgattata cacgtgcgtt gaatgccgaa
     1261 gagccagata cagcaagtgt aactgatttt atttttgcgc catctccaat taccgaagaa
     1321 aaaggagaac gtgaaccaaa aggaaagaaa aaaacagtaa tggtagattc tgccttgttt
     1381 gctgttcgtg agatattaga aaaacatcca gaagcattgt tgtatggaca agatgtgggt
     1441 ggacgtttag gcggtgtgtt ccgtgaggct gcaaccttgg cacaacaatt tggtgataac
     1501 cgtgtgttta acacgccaat tcaagaggca tttatcattg gttcaactgt gggaatgtcg
     1561 gctgctggat tgaaacctat tgttgaagtt caatttgccg attacatttg gccaggactc
     1621 aaccagttgt ttaccgaagt ttcgcgctcc aattatttat ccaatggaaa atggccggtg
     1681 agttgtgtta ttcgtgtgcc aattggtgct tatggttcgg gcggacctta ccattcatcg
     1741 agtgttgaat cggttttaac aaatattcgt ggggtgaaaa cggtttatcc ttcaactggt
     1801 gcggatttga aagggttgtt aaaagccgct tattacgatc caaacccagt agtaattttg
     1861 gagcacaaag gtttgtattg gtcaaaaatc aaaggaactg aaggtgccat gtcggttgag
     1921 ccagatgaag attacgttat tccgctaggt aaaggtagaa ttgtacaaca tgccgatgaa
     1981 aaagtagtta aaaatggaga atcgtgtgtt gtaattacct acggtagagg tgtttattgg
     2041 acgttggagg cttgcgaaac gtttaaagat aaagtagaaa ttgttgattt acgcaccttg
     2101 catccgttgg attttgagtt aatcaatgaa atggctcaaa aacacagcaa agtattgttg
     2161 gtaaccgaag aatcggtaga agcatcgttt actttaggat tggcaggaag aattcaacgc
     2221 gataacttta aagcattgga tgcgccagtt gcaattgttg gttctgttga tacaccagca
     2281 attccgatga attcatcgtt ggaagcaact ttattgccaa atgccgataa agtgaaaaaa
     2341 gctttggaag atttattagc ttactaaaaa taacatttaa tagttggaaa atccgtaatg
     2401 cttaagtgtg ttgcggattt tttttatatt gaatctggtc tgttgaaaaa caattcttgt
     2461 gttgtagccg actcctgcat ggattgaatt acagaatcaa gtggtttggc ataaaccgtt
     2521 ggataatcta cttcacctaa gtgcggatga atagtgtagg caagttgctc cggttcaata
     2581 gaaaattgag aatttactcc aggtttatta ccacgaggat ctactctaaa ccagccgtat
     2641 tctctcaaat aaatagcatt caatccatgc aaggcaaaac ctgagttttc atctgtcgga
     2701 acacgtaaaa cacgttgata gcaaaaccct gctggaattg aaagtccgcg caatagtgcc
     2761 accaataaat gtgatttaga aaagcaaatt ccttcggatg atttcagcac atcttcggca
     2821 ttgatggtta ccaacggatt tttggtatca aacgaatgaa aaatatcgtc gcgtgcaatt
     2881 tcaaatgcaa ttctagcctg tttttcaggg ttagaggttt gctctttaat ccattcaatt
     2941 tcttgactta tcataggtgt gttgaacgtt atgataggtg gaatttcagc taaataatcg
     3001 tcaatattgg gtgactcaat gataagtttc atgtcagtga tttttgtttg ataaagttac
     3061 agatatttta agaaaattac aattttgggt tgatttaact atttttatgt ttatcaactt
     3121 actatttgtt ttaattttgg aaataaacaa tattaaaatg aataaattat tgactaccat
     3181 tttagccgct tctgttgtga gtttagcgag cgcgcaagta aaaacaccaa aggcaagtgc
     3241 acatgcagaa ttggaggcaa ctgttggttt aacagaaatt gaagttgagt attctcgacc
     3301 aaacaaaaat aaccgtgtgg tttttggcga cgtagttccg tttgataaaa tttggagaac
     3361 aggagccaac aaaaatacaa ccattgaatt ttcggatgat gtgacagtgg gtggtgctcc
     3421 actaaaagca gggaaatatg ccatttatac caaaccagga aaatctgctt gggaagttta
     3481 tttttataca gatactgaga actggggaaa tccacaaact tgggacgatg caaaagttgc
     3541 cgctaaagca gtagcaaaag ttgaacaatt gtcacaacca gttgaaacat tcactatttc
     3601 tgttgatgac ttgaaaatgg acaaagcaaa tttgacgttc tcatgggata aaacacgtgc
     3661 agttttacct attgttgttc caacagatgc aaaagtgatg gaaacaattg aaaaaacaat
     3721 gaatgcagaa ccaaaagcgg gtgattttat tgcagctgcc aattattatg caagtacagg
     3781 aaaagatgct caattggcaa aagattggat ggataaaggg atgagtttaa tgaaagaacc
     3841 tcaatattat cattaccacc aacaagcagt agtatatgcc aatgcaggcg attttaaagg
     3901 agccgttgaa atagctaaaa aatcattgga aggcgcaaaa gctgctggcg atgatgctta
     3961 tgtaaaaaag aatgaagttg ccattaaaga atgggcaaat aagaaataaa ctcaattttt
     4021 acaatcaaag aatcgtgtaa aaaaacgcga ttctttgatt tattctaaac tatttttatg
     4081 aatacttatt tt
//
LOCUS       DAOQVQ010000178         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_24658_length_4078_cov_3.595575, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000178 DAOQVQ010000000
VERSION     DAOQVQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24658_length_4078_cov_3.595575"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..517)
                     /locus_tag="PKN22_06380"
     CDS             complement(125..517)
                     /locus_tag="PKN22_06380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNS42368.1"
                     /translation="MEKRIIDPWVWGPQTNSAQAVEVTNVAGTLYCSGQVALDENGVP
                     SNADMRTQLLQTIKNLEQLIQEAGYDCKNIVRLNIYTTNMQEFFATCMDVYVPFIQQN
                     GIKQATSMIEVKGLFATLTVELEATVVK"
     gene            complement(593..1285)
                     /locus_tag="PKN22_06385"
     CDS             complement(593..1285)
                     /locus_tag="PKN22_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNS42369.1"
                     /translation="MQDTDLKRLSRLTAILTQLQTKRLLTASSLAEKFNVSTRTIYRD
                     IKALEDAGVPIITEEGKGYSLMEDYKIPPIMFTENQANALILAEQLVLTTKDISFIKD
                     YTEAIEKVKAVLKYSLKDKVNLLSERTRFQRNINRERNSNNLSDLQFALTNHILVKIE
                     YTNEKGENSTRTIEPFALLNTENWLLVAYCRLRKEFRYFRLDRIQKMQFLEEHFEPHK
                     MTLQEYFEKYYD"
     gene            complement(1298..1453)
                     /locus_tag="PKN22_06390"
     CDS             complement(1298..1453)
                     /locus_tag="PKN22_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42370.1"
                     /translation="MLLGTPRIIIRNFILDDLTDFHRYRSNPEVTKYQGFAKVAMLNR
                     EWNDIKN"
     gene            complement(1492..2046)
                     /locus_tag="PKN22_06395"
     CDS             complement(1492..2046)
                     /locus_tag="PKN22_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNS42371.1"
                     /translation="MQSENLIQSLIEQTRQVINQAEKFQQLEINSLTWKANPTSWSVL
                     ECLEHLNLYGDFYLPEMKQKIQASSTQPEVSFKPGLLGDYFAKSMLPKEKLNKMKTFK
                     DKNPLNAALDKSVIDKFIHQQMELLDLLNASRKVSLNKVKIQTSISSLIKLKLGDTFR
                     FYINHIIRHLKQADNVLQNMNSTN"
     gene            complement(2102..2608)
                     /locus_tag="PKN22_06400"
     CDS             complement(2102..2608)
                     /locus_tag="PKN22_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS42372.1"
                     /translation="MNAIFDLSHKTIQLERNEFLKVKGSIDTNVYFVENGSLRIFILD
                     DYEEQTIRFGYQGNIIVSLDSFLSGKPSDLYIQALKKTTVKVISKSNFLKFIEEKENR
                     DWWLNTLENLVLQQMEREVDLLTNSPKERFERVLKRSPQLFQEIPHKYIANYLRMSAE
                     TLSRLKKS"
     gene            2708..3628
                     /locus_tag="PKN22_06405"
     CDS             2708..3628
                     /locus_tag="PKN22_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance efflux transporter family
                     protein"
                     /protein_id="HNS42373.1"
                     /translation="MMRNKQWAITISLVSALFFATTFVLNRQISLNGGSWMWSASLRY
                     LWMIPFLLIIVLLKKQLRPLFIEIKAQPKQWFLWSFVGFGVFYSLLTFSASYAPSWLV
                     ASTFQITIIAGMLMAPFINKTSYSSTFSLPTFIFSILMLIGVLITQLNQAKAVSLNEL
                     LLGTVPVLIAAFAYPLGNRKMMQITKGRLNSVQRTLGMTLCSLPLWIGLIMVEISQNH
                     LPSYSQLSQTLIIGLFSGVVATTMFFWATDLVRKNEESLAAVEAVQSSEVVFAVLGEV
                     FILKAQFPDTISMIGITLIIVGVVLHSLKK"
     gene            complement(3810..>4078)
                     /locus_tag="PKN22_06410"
     CDS             complement(3810..>4078)
                     /locus_tag="PKN22_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42374.1"
                     /translation="TTVSLHEQAALKAIQVYPNPASSVISIASEQTEIVALTLMDNAG
                     RIVYHAAPTNTNYQIPVAQLTNGLYVLHLETKQGTTVRLVEVQH"
BASE COUNT         1201 a          661 c          715 g         1501 t
ORIGIN      
        1 aaatttaccc gatgctttgg tatgaaaaac aagccataca gccgtttcag aaagatgatt
       61 ttgtgctaac cattcttccc acgctgcacg acttgctgga tagaaaacta aggtttctga
      121 catgttattt taccactgtt gcttctaatt caacggttaa agtagcaaat aatcctttaa
      181 cttcaatcat tgaagtagct tgtttgatac cattttgttg aatgaaagga acataaacat
      241 ccatacacgt tgcgaaaaac tcttgcatat tggtcgtgta aatattcaac cgaacgatgt
      301 ttttgcaatc gtaacctgct tcttgaatca gttgctctaa gttcttaatg gtttgtagca
      361 attgtgtacg catatcagca ttgcttggca ctccgttttc atcaagtgct acttgcccag
      421 aacagtaaag agttcccgct acattggtta cttcaacagc ttgtgccgag ttggtttgtg
      481 gaccccaaac ccaagggtcg atgattcttt tttccatttt aattttgttt taatttttat
      541 atactgcaaa actaaaatgg ggtagtgaca gccttatgtc ataggttgaa aatcaatcgt
      601 aatatttttc aaaatactct tgcaacgtca ttttgtgcgg ttcaaaatgc tcttctaaaa
      661 actgcatttt ctgaattctg tctaaacgga aataacgaaa ttcttttcgc aaacgacaat
      721 aggcaactaa taaccaattt tcggtgttga gtaaagcaaa cggttcgatg gttcgtgtag
      781 agttttcacc tttttcgttg gtatattcaa tcttgacaag aatatgattg gtaagtgcaa
      841 actgtaaatc cgaaagattg ttactattgc gttctcgatt tatatttcgt tggaatctgg
      901 tacgttcaga cagtaggttt accttgtctt ttaggctgta ttttaaaacg gctttcacct
      961 tttcaatggc ttcggtgtaa tctttaatga acgaaatgtc tttggtggtc aagaccaatt
     1021 gttcagctag aatcaaggca ttggcttggt tttctgtaaa cataatcggt ggaattttat
     1081 aatcttccat tagcgaatat cctttgcctt cctctgtgat gataggcact ccagcgtctt
     1141 ctaatgcttt gatgtcgcgg taaatggttc gggtactgac attaaatttt tcagccaaag
     1201 aacttgccgt caatagcctt ttagtttgca attgggtgag tattgcagtc agtcgagaaa
     1261 gtcgttttaa atcggtatct tgcatggatt ggttaactta gttttttata tcgttccatt
     1321 cacgatttag cattgctact tttgcaaatc cttggtattt tgtaacttca ggattagacc
     1381 gataacgatg aaaatcggtt aaatcatcca agataaagtt tctaattatt attcgcggag
     1441 ttccgagtag cattttataa aacggttttc aacaaaatta atcatttcaa tctaatttgt
     1501 tgaattcata ttttgcaata cattgtctgc ttgttttaaa tgacgaataa tgtgattgat
     1561 gtaaaaacga aatgtatcgc ctagtttaag ttttattaga cttgaaatag aagtttgaat
     1621 ttttactttg ttcaaactta cttttctaga cgcattcaat aaatctaaca attccatttg
     1681 ttgatggata aatttgtcaa tcactgattt gtctaaagct gcgttgagag gatttttatc
     1741 cttaaaagtt ttcattttgt tgagtttttc tttaggtaac atactttttg caaagtagtc
     1801 acctaaaaga cctggtttaa atgaaacttc tggttgagtt gatgaagctt gtattttttg
     1861 tttcatttct ggtagataaa aatctccgta caaattcaaa tgctccaaac attccaaaac
     1921 actccaagat gtagggtttg ctttccatgt taacgagttt atttcaagtt gttggaattt
     1981 ttctgcttga ttaataactt gtctcgtttg ctcaattaag gattgaatta aattttctga
     2041 ttgcataact attttttttt gcaaagttct tatgttagaa ttacttaaat cttgattcaa
     2101 atcaagattt tttcaaacga gataaggttt ctgcactcat tcgtaaataa tttgcaatgt
     2161 atttatgagg aatttcttga aacaattgtg gacttcgctt taagacacgt tcaaagcgtt
     2221 ctttcggtga attggttaat aaatccactt cgcgttccat ttgctgaagc actaagtttt
     2281 ctagtgtatt tagccaccaa tctcggtttt ctttttcttc aataaatttt aaaaaatttg
     2341 acttactaat aactttaacg gttgttttct ttaatgcttg aatgtataaa tcggaaggtt
     2401 ttccagaaag aaatgaatct agtgaaacga taatattgcc ttgataacca aaacgaatgg
     2461 tttgttcttc gtaatcatct aagataaaga ttctcaaact cccattttca acaaaataaa
     2521 cattcgtgtc aatactccct tttactttaa ggaattcatt acgttctaac tggatagttt
     2581 tgtgtgaaag atcgaatata gcattcattt agaaagttca gaattaaaag ttttttattt
     2641 caaaaatcaa gcttgtacta caatattaaa tagttttgtc gtaaaattct acgttttatt
     2701 aaattttatg atgagaaaca aacaatgggc tattacaatt agcttagtgt cagctttatt
     2761 ttttgcaacc acatttgtat tgaataggca aatctcatta aatggaggca gttggatgtg
     2821 gagtgcttct ctgaggtatt tatggatgat accgttttta ttgatcattg tcttattaaa
     2881 aaagcaatta agaccattgt ttatcgaaat aaaagcacaa ccaaagcaat ggtttctgtg
     2941 gagttttgta gggttcgggg tgttttattc tttacttact ttttctgcta gttatgctcc
     3001 ttcgtggttg gttgcctcaa cgtttcaaat aacgataatt gccggaatgt tgatggctcc
     3061 ttttattaat aaaactagct attcatctac tttttcattg cctacattta ttttttcaat
     3121 cttgatgtta ataggtgttt taattacaca gcttaatcaa gctaaagcag taagtttaaa
     3181 tgagttatta ctaggaaccg ttccagtttt aattgctgcg tttgcctatc ctttagggaa
     3241 tagaaaaatg atgcaaatca caaaaggaag actcaattct gttcaacgaa ctttaggaat
     3301 gaccttatgc agtttacctt tgtggattgg actaataatg gttgaaattt cacaaaatca
     3361 tttaccttct tacagccaat taagccaaac attaataata ggacttttct caggtgtggt
     3421 agcaactacc atgttttttt gggctacaga tttagtaaga aaaaatgagg aatctttagc
     3481 agcagtagaa gcagttcaat cttcagaagt tgtttttgca gtattaggag aggtattcat
     3541 cttgaaagct caattcccag acacaatttc gatgattggg attacattaa ttattgttgg
     3601 agttgtattg catagcttaa aaaagtagaa acctcagttc aatttttttt tgacttttct
     3661 cataaaataa tcttctcaat attgcttgaa tgtcaagatg tctgtcaaac ttggctttta
     3721 aatcgttgga aataatgagt tcttctacga tgttgtataa aaaaacggcc atccatttcg
     3781 gacagccgtt catgattttt gttcaaaaat taatgttgta cttctactaa acgaaccgtt
     3841 gttccttgct tggtttcaag gtgcaataca tacaatccgt tggttaattg cgcaactggt
     3901 atttggtaat tcgtgtttgt tggtgctgca tgataaacga ttcttcctgc attgtccatc
     3961 aacgttaggg caacaatttc cgtttgctca cttgcaatag aaataacact tgacgctgga
     4021 ttaggataaa cttgaatggc ttttaacgcc gcttgttcgt gaagtgaaac agttgtaa
//
LOCUS       DAOQVQ010000179         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_24811_length_4061_cov_3.535197, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000179 DAOQVQ010000000
VERSION     DAOQVQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24811_length_4061_cov_3.535197"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3055
                     /locus_tag="PKN22_06415"
     CDS             <1..3055
                     /locus_tag="PKN22_06415"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42375.1"
                     /translation="LTYTYIIAATGCEASDNKIVTVVNPAQANAGLDQVICVVNSTVQ
                     VVGTPATGTWSGVGVDAAGNFTTATVGNFELVYTNGQGNCLTRDTMYFTVHPLPIVNA
                     GPDVDICVSSSSITLNATPTGGVYAGTGIVNTTTGEFNPTLATVGSHTIVYTYTDANT
                     CVNYDSLVINVQPLPVVDFDVDSIVCLNTSVPFTNNSTLIAQANWNFGDGATSNAINP
                     THTYTLSGIYTVQLVITSPFGCVDSISKNLEVYVPPVAAFVVTPDSLCGPLTAAFTNN
                     STGPDVSYLWDFGNGTTSTVANPPAVVYPAGILADTTYTISLTVSNYCGQVTTQNNVI
                     VMPQPVAVFGTNFNSYCSPWTPEFANTSYGLPDTYSWDFGNGTTSNTSDDLFQIPVYT
                     TGTDTTSYTITLTVTNECGTSTAQHTITVLPNTVNSFFNTSVVEGCENLSVDFTQYTL
                     GSTSYHWDFGDGNTSTDYSPSHTFTQAGTYNVSLMVNNGCSYDTTTVAIVIHSSPNVA
                     FIHQPDSVCSFTPIQFINQSDVLANYAWSFGDGGTSNLTNPTHTYAATGNYTVTLTGT
                     SLDGFCTVTATEIVHINAPPTANFTINPSSGCVPLTVQFTNQSVGAAYSYWDFGDGNT
                     TVNTNPQHTYTTAGTYTVLLIVQGVNGCVDTLAQYVNVNPIPTAAFSYQPAGAMCGPN
                     LQAQFTNASTGAVSYEWSFGDGGVSNQNNPNYTYQTIGTYTIQLIATNQFGCKDTTQQ
                     VITIHQTPVASFTTPSVLGCQNYDIPFTSTSQFADSLIWNFGDGNTANGTAVTHSYSE
                     VGSYTVSIIAIGSGGCADTFVMTTPIDIQTSPTAAFTYETSIENITNATVIFTNQSTN
                     YTSSVWDFGNGESSTETNPIHNYYEHSDFFVMLVAINDNGCTDTVELLVKNPLQQGLF
                     VPNAVSPSHSDYEVSHFLPKGIGLKEYHVQIYDDWGNLIWESTKLDELGRPVEGWDGT
                     YRGEPVQQDAYVWKISATFLDESIWEGKEYPKGKIKKSGTVTVIR"
     gene            3059..>4061
                     /locus_tag="PKN22_06420"
     CDS             3059..>4061
                     /locus_tag="PKN22_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS42376.1"
                     /translation="MKNVILYIAIIFTAVQLNAQDPNFSQFYNNPVYYNPGMVSLNSG
                     YSVKLHARNLWAPVPGRFNTFAASFEGQIIPKLAMAANVYTDVAGEALLRTTGGYLTY
                     AYRPVETKNFMLQVGVSGGIINKFIDWSKLRFSDNYHEIQGEVGTTAFIPPSYRSTTY
                     ADFGSGIVVRFNHEARKYSGYRKMMATFGFSAMHLTQPKEGLMFGGKLPIKLVGSFNT
                     AILVNETIISPAVIYETQNKFQTATIGFSVIQRPIVVGVWVRNEGLFRRANKFDSFIL
                     NFGVNFPTKSKQTIKVMYSVDFTISKLRSASFGSHELSLIYDWDDRFMSQKHRMKRSR
                     "
BASE COUNT         1274 a          742 c          784 g         1261 t
ORIGIN      
        1 attgacatac acctacatta ttgcggcaac aggatgtgag gcatccgata ataagattgt
       61 caccgttgta aatccagcac aagccaatgc aggattagat caagttatat gtgtggttaa
      121 ttcaactgtg caagttgttg gaacgccagc tacaggtacg tggtcgggtg ttggtgttga
      181 tgcagcgggg aattttacaa ctgcaacggt tggtaatttt gaattggttt acaccaacgg
      241 tcaaggcaac tgtttaacac gagatacgat gtattttaca gtgcatccat tgccaattgt
      301 gaatgccggt cctgatgtag atatttgtgt ttcatcatct tcaataacat taaatgcaac
      361 gccaacagga ggagtatatg ctgggactgg aattgtgaat accacaacag gtgaatttaa
      421 ccctacatta gcaactgttg gttcacacac cattgtttat acttacacag atgcaaatac
      481 atgtgtaaac tatgattctt tagtcatcaa tgttcaacct cttccagttg tagattttga
      541 tgttgactct attgtttgtc tcaacacaag tgttccattc accaacaaca gtacgttaat
      601 agcgcaggca aattggaatt ttggtgatgg tgctacatcc aatgccatta atccaacaca
      661 tacttatact ttaagtggga tttatacagt tcaattggtt attactagtc cttttggatg
      721 tgtagattct atttctaaaa acttagaggt gtatgtaccg ccagttgcag cttttgttgt
      781 aacgccagat tcattgtgtg gaccgttaac tgctgcattt acaaacaatt caacaggacc
      841 agacgtaagt tatttatggg attttggaaa tggaactaca tcaactgttg caaatccacc
      901 tgctgttgta tatcctgctg gaattttagc agatacaacc tacaccattt cattaacggt
      961 tagtaattat tgtggacaag ttacaactca aaacaatgtt attgtaatgc cacagcctgt
     1021 tgcggtattt ggaacaaact ttaattcata ttgttcgcca tggactccag aatttgcaaa
     1081 cacaagttat ggattaccag atacatattc ttgggatttt ggaaatggaa cgacttctaa
     1141 tacttcagac gatttgtttc aaattccagt ttatacaaca ggaaccgaca ccacttccta
     1201 tactatcact ttgactgtaa caaatgaatg tggtacaagt acagctcaac acaccataac
     1261 tgtattgcct aatactgtaa attcattttt caatacatcg gttgttgaag gatgtgaaaa
     1321 tttatcggtt gattttacac aatatacatt aggaagtacg agctatcatt gggattttgg
     1381 agatggaaac acatcaacag attacagtcc atcgcacaca tttacgcaag ctggaaccta
     1441 caatgtatcg ctgatggtaa ataatggatg tagctatgat acaacaacag ttgctattgt
     1501 tattcattcg tcaccaaatg tggcatttat tcaccaacca gattcagttt gtagtttcac
     1561 cccgattcaa tttatcaacc aatcggatgt gttagccaat tatgcttggt cgtttggaga
     1621 tggaggaaca tcaaatttaa ccaatcctac acacacatac gcagctacag gaaactatac
     1681 tgttacttta actggaactt cattggatgg tttctgtact gttactgcta cagaaattgt
     1741 acacattaat gcaccaccaa ctgctaattt tacaattaat ccgtcgtcag gatgtgtgcc
     1801 actaactgtt cagtttacca atcaaagtgt gggagcagcc tattcttatt gggattttgg
     1861 tgatggaaat actacggtga ataccaatcc acaacatacc tacacaacgg caggtacgta
     1921 tacggtttta ttaattgtac aaggagttaa cggatgtgtt gataccttag cgcaatatgt
     1981 aaatgttaac ccaataccaa cagcagcatt tagctaccaa ccagcgggtg caatgtgtgg
     2041 accaaatctt caagctcaat ttaccaatgc atcaactggc gcagtaagtt atgaatggag
     2101 ttttggtgat ggtggggttt ccaatcaaaa taacccaaat tatacctatc aaacaattgg
     2161 aacatataca atccaattaa ttgcaacgaa ccagtttgga tgtaaagaca ccacacagca
     2221 agtaattact atccatcaaa caccagttgc aagttttaca acaccatcgg tattaggatg
     2281 tcaaaattat gatattcctt tcacttcaac cagccagttt gcagattcgt tgatttggaa
     2341 ttttggcgat ggcaatacag ctaatggaac tgctgttaca catagctata gtgaagtagg
     2401 ttcctatacc gtatcaatca ttgcaattgg ttctggagga tgtgctgata cattcgtaat
     2461 gacaactcct attgatattc agacatcacc aacggctgca tttacctatg aaacgtctat
     2521 tgagaacatc accaatgcaa cagtgatttt taccaatcaa tcaaccaatt atacatcatc
     2581 agtttgggat tttgggaatg gagaatcatc cacagaaaca aacccaattc ataactatta
     2641 tgagcacagt gatttctttg taatgcttgt tgcaattaat gataatggtt gtacggatac
     2701 agttgaatta ctagtaaaaa accctttgca gcaaggtttg tttgttccta atgcagtatc
     2761 gccaagtcac agtgattacg aagtttctca tttcttaccc aaaggaattg gcttgaaaga
     2821 ataccatgtt cagatttacg atgattgggg caacctgata tgggaatcaa ccaagttgga
     2881 tgaattagga agaccagttg aaggttggga tggaacttac cgtggagaac ccgttcagca
     2941 ggatgcttat gtttggaaaa tttcggctac attcttagat gaaagtattt gggaaggaaa
     3001 agaatatcct aaagggaaaa ttaaaaaatc aggaacagta acagtaatca gatagaaaat
     3061 gaaaaacgtt atcttatata ttgcaattat ttttacagct gttcagttga atgctcaaga
     3121 ccccaatttt tctcagtttt ataataatcc cgtttattat aatcccggta tggtgtcgtt
     3181 aaacagcgga tattcggtta aattgcacgc acgtaatttg tgggcacccg ttccaggtag
     3241 gtttaatact tttgcagctt cgtttgaagg acaaataata cccaaactag caatggcagc
     3301 aaatgtttat accgatgttg ctggagaagc attacttaga acaacaggtg gttatttaac
     3361 ctatgcttat cgccctgtag agacaaaaaa ctttatgtta caagtggggg taagtggtgg
     3421 aattatcaat aaattcattg attggtcaaa attgcgtttt tctgataatt accacgaaat
     3481 tcaaggtgag gtgggtacaa cagcatttat tccacctagt tatagaagta ccacctatgc
     3541 cgattttggt tcgggaatag tcgtgcgttt taaccacgaa gcacgtaaat acagtggata
     3601 tcgtaaaatg atggctacat ttggattttc agcaatgcat ttaacacaac ctaaagaagg
     3661 tttaatgttt ggagggaaat tgccaatcaa attagttggt tcttttaata cagctatttt
     3721 ggttaatgaa acaattatct cacctgctgt gatatatgaa acccaaaata agtttcagac
     3781 tgcaaccatt ggtttttctg taattcaacg ccccattgta gttggtgttt gggttagaaa
     3841 cgaaggtttg ttccgtcgcg ccaataaatt tgattcgttt atcttaaatt ttggtgttaa
     3901 tttcccaact aaatcaaaac aaacaataaa agtgatgtat agtgtggatt ttactatttc
     3961 caaattgcgg tcagcttctt ttggttcgca tgaattatca ttgatttatg attgggacga
     4021 tcgttttatg tcacaaaaac accgtatgaa acgcagtaga a
//
LOCUS       DAOQVQ010000180         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25224_length_4013_cov_4.080344, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000180 DAOQVQ010000000
VERSION     DAOQVQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25224_length_4013_cov_4.080344"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKN22_06425"
     CDS             complement(<1..736)
                     /locus_tag="PKN22_06425"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNS42377.1"
                     /translation="MPSSVINPPTNFSLDPFVGVWTASEASHLLRRTLFGPTYQNIAD
                     SVANGMDATVATLLTPTSTDLPLTYDAGEAVASFGQPWNLTPQSSNASLIPPTETARN
                     KSLGAWAMKNLNNESMSITEKMCLFWHNHFAAPFSVDARSTLKYHQLIRQYCLGNFKD
                     FVKAMTINTSMLEFQNGATNNVYSPNENYAREFLELFTIGKGLQVGPGDYSNYTEEDV
                     AAGAKIFTGYYLQGIQSSTDNDVTSAF"
     gene            917..3121
                     /locus_tag="PKN22_06430"
     CDS             917..3121
                     /locus_tag="PKN22_06430"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNS42378.1"
                     /translation="MSNKSKIFYTKTDEAPLLATYSFLPIVNAFASKANIEVELKDIS
                     LAGRILANFTNYPDALTELGKLTANADTNIIKLPNISASIPQLKGAIAELQKAGHAIP
                     NYPDDAKTEEELKVKATYAKILGSAVNPVLREGNSDRRAPKAVKNYAKANPHSMGAWS
                     AASKTKVAHMTGGDFYGTEKSVTVENDTQFKIEFVAQDGSVKELKGLSALKAGEVIDS
                     SVMNLNALKAFVAQTIEETKKTGVLLSAHLKATMMKVSDPIIFGAIVEVYFKEVFEKY
                     AAVFAELNINKNNGLGEVFAKIAGHSKETEIKAAIDAAITNGPDLAMVNSDKGITNLH
                     VPSDVIVDASMPAMIRIGGKMWDKNGKEQDTIAMIPDRSYAGVYVAVIDDCKKNGAYD
                     PTTMGSVPNVGLMAQKAEEYGSHDKTFQAEANGTIRVTDANGVVFMEQAVEAGDIFRM
                     CQTKDAPIQDWVKLAVNRARLSETPAVFWLDENRAHDREIIKKVNKYLANYDTTGLDI
                     RILNPVDATLFSVERIRKGLDTISVTGNVLRDYLTDLFPILEVGTSAKMLSIVPLMNG
                     GGLFETGAGGSAPKHVEQFLEEGYLRWDSLGEFLALGASLEHLSQVENNPKAMVLAET
                     LDQATEKFLANDKSPARRVGQIDNRGSHFYLAMYWAQALAAQTKDAELASQFSAVAND
                     LTSNEAKINEELIGAQGKAQNIGGYYFPSDELAAKAMRPSATLNQAIDSLTK"
     gene            3271..3552
                     /locus_tag="PKN22_06435"
     CDS             3271..3552
                     /locus_tag="PKN22_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42379.1"
                     /translation="MENVSKFQKQVQWFEFYRYGAMANMLIFISCLGAVASMLSIKAH
                     FDMGMYIAAVVSMANLVALIAQVKAEYCLKIFYGALAVNVSIMLATLFM"
     gene            3557..3940
                     /locus_tag="PKN22_06440"
     CDS             3557..3940
                     /locus_tag="PKN22_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNS42380.1"
                     /translation="MKKEITNIEDVKLLVNEFYDRVRHDEKLGYIFDDVIQDRWPMHL
                     EKMYSFWQTVILGDHTYFGAPFPPHAKLPVEKAHFDRWMELFYGTLDDYFEGESVDKA
                     KWQAKRMAYVFQEKIEAIKNNQLSI"
BASE COUNT         1371 a          645 c          805 g         1192 t
ORIGIN      
        1 gaaacgcact tgtcacatca ttatctgtac tgctttgtat tccttgtaga taataacccg
       61 taaaaatttt agcacctgca gctacatctt cttcggtgta gttagaataa tcgcctggtc
      121 caacttgcaa tccttttcca atagtaaaca attccaaaaa ttcacgtgca taattttcat
      181 tgggagaata cacgttgttg gttgctccgt tttgaaattc caacatactg gtatttatcg
      241 tcattgcttt gacaaaatct ttaaaatttc ctaagcaata ttgacgaatt aattgatggt
      301 atttaagtgt agaacgtgca tctaccgaaa aaggcgcagc aaaatggttg tgccaaaaca
      361 aacacatttt ttcggtgata ctcattgatt cgttattcaa atttttcatt gcccatgcgc
      421 ccaatgattt gtttcgagcg gtttctgtgg gtggaattaa tgacgcattg gatgattgcg
      481 gagttaaatt ccatggctga ccaaaacttg caacagcttc acctgcgtcg tacgttaatg
      541 gtaaatcggt gcttgttggt gttaataatg ttgcaacagt tgcatccatc ccgttagcaa
      601 cggaatcagc aatgttttga tatgttggac caaacaacgt tcttctcaac aaatgagaag
      661 cttctgatgc tgtccacacc cctacaaacg gatctaaact gaagtttgta ggcggattga
      721 ttacagaact tggcataaac agttagttta aatagttaac caaatataaa acaaaatgat
      781 agttacacaa aatttttaac aaaatgttct atttttttca aaacatctaa taaaaaagac
      841 ttgtaaatct agttagatac attaaatttg tacgcgtagt taaataatag tagttaatcg
      901 aatataaaaa cataaaatga gcaataaatc aaagattttt tacaccaaaa cagatgaggc
      961 gccattattg gcaacgtatt cttttttacc aattgtaaat gcatttgcat caaaagcaaa
     1021 cattgaagta gaattaaaag atatttcttt agcgggaaga attttagcca attttactaa
     1081 ctatccagat gcgttaacag aactaggaaa gttaacagct aatgcagata ccaacatcat
     1141 taaattaccg aatatttctg catctattcc gcagttgaaa ggagctattg ctgaattgca
     1201 aaaagcggga catgcaattc ctaactatcc agacgacgca aaaacagaag aagaattaaa
     1261 agtaaaagca acgtatgcta aaattttagg gtcggctgta aatcccgttt tacgcgaagg
     1321 aaactctgat cgtagagctc ccaaagcagt aaaaaattac gcaaaagcaa acccacattc
     1381 aatgggtgct tggtcggctg cttctaaaac aaaagttgca cacatgactg gtggtgattt
     1441 ttacggaaca gaaaaatcgg taactgtaga aaatgataca cagtttaaaa ttgaatttgt
     1501 tgctcaagac ggaagtgtaa aagaattgaa aggcctttca gcattaaaag caggagaagt
     1561 tattgactca tcagtaatga accttaatgc attgaaagct tttgttgcac aaacaattga
     1621 agaaacgaaa aaaacaggtg ttttactttc agcacactta aaagcaacga tgatgaaggt
     1681 ttcagaccct atcatttttg gtgcaattgt ggaagtttat ttcaaagaag tttttgaaaa
     1741 atatgcagct gtttttgcag aattaaatat taataaaaac aatggtttag gcgaggtttt
     1801 cgctaaaatt gcaggtcact cgaaagaaac cgaaattaaa gcggctattg atgcagcaat
     1861 tacaaatggt cctgacttgg ctatggttaa ctccgacaaa ggaattacca acttgcacgt
     1921 gccatctgat gtaattgtgg atgcatccat gcctgcaatg attcgtatcg gcggaaaaat
     1981 gtgggacaaa aacggaaaag agcaagatac tattgctatg attccagatc gttcttatgc
     2041 aggggtttat gtagctgtaa ttgacgattg taagaaaaat ggagcttatg accctacaac
     2101 tatgggttct gttccaaacg tgggtttaat ggcacaaaaa gcagaagaat acggatcaca
     2161 cgataaaact ttccaagcag aagccaacgg aaccattcga gtaacggatg caaacggagt
     2221 tgtgtttatg gaacaagctg tagaagctgg cgatattttc cgtatgtgtc aaactaaaga
     2281 tgctccaatt caagattggg taaaattggc agttaacaga gcacgtttat cagaaactcc
     2341 agcagtattt tggttagacg aaaacagagc acacgacaga gaaatcatca aaaaagtaaa
     2401 taaatattta gcaaattacg ataccacagg attggatatc cgtattttaa acccagttga
     2461 tgcgacattg ttctctgttg agcgcattcg caaagggttg gatactattt cagtaacagg
     2521 aaacgtgttg cgtgattatt taacagactt attcccaatt ttagaagttg gaacatcagc
     2581 aaaaatgttg tccatcgttc cattaatgaa cggaggtggt ttgtttgaaa cgggagctgg
     2641 tggttctgca cctaaacacg tggagcaatt cttagaagaa ggctacttgc gttgggattc
     2701 attgggtgaa tttttggcgc tgggagcatc tttggagcat ttatcgcaag tagaaaacaa
     2761 tccaaaagca atggtgttgg cagaaacgct tgatcaagca acggagaaat tcttagcaaa
     2821 cgataagtcg cctgcacgta gagtggggca aattgacaac cgtgggtcgc atttctattt
     2881 ggcaatgtat tgggctcagg cattggcagc acaaacaaaa gatgcagaat tggctagtca
     2941 gtttagtgct gttgcaaatg atttgacatc aaacgaagcg aaaattaatg aagaattaat
     3001 tggtgcgcaa ggcaaagcac aaaacattgg tggatattat ttcccaagcg atgaattggc
     3061 agcaaaagca atgcgtccga gtgctacttt gaatcaagcg atagatagct taacaaaata
     3121 agacataaat tattcaaaga aattttaata tttcttatat atttgcaatt tagccgtgct
     3181 tatgtgcggc taaattattt atttaaaagc aatttattca gcgttaactc gttgaattaa
     3241 aataaattac aaaatttaaa ataaaaagtt atggagaatg taagtaagtt tcaaaaacaa
     3301 gttcaatggt ttgaatttta ccgttatgga gctatggcta atatgcttat tttcataagc
     3361 tgtttaggtg ctgttgcgtc aatgttatca attaaagctc attttgatat gggaatgtac
     3421 attgcagcag tagtttcaat ggcaaacctt gtagcattaa ttgcgcaagt gaaagcagaa
     3481 tattgtttga aaattttcta tggagcatta gcagtgaatg tttcaataat gcttgctaca
     3541 ttgttcatgt aatcttttga aaaaagagat aacaaatatt gaagacgtaa aattgctcgt
     3601 taatgaattt tacgatcgtg taagacatga cgagaaattg ggttacattt ttgacgatgt
     3661 gattcaagac agatggccaa tgcatttaga aaagatgtat tcgttttggc agacagtgat
     3721 tttgggcgac cacacctatt ttggcgctcc atttccaccg catgctaagt tacctgttga
     3781 gaaagcacat tttgatagat ggatggaatt gttttatggt acgctggatg attattttga
     3841 aggtgaatct gttgacaaag ccaaatggca agcaaaaaga atggcgtatg tttttcaaga
     3901 aaaaattgag gctattaaaa acaaccaact gtcaatttga taaataattt ttttcttttt
     3961 ttgcaataat taaagaaaca tagcgttaag tgttttaaga attaaaattt aat
//
LOCUS       DAOQVQ010000181         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25228_length_4013_cov_3.593987, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000181 DAOQVQ010000000
VERSION     DAOQVQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25228_length_4013_cov_3.593987"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="mutL"
                     /locus_tag="PKN22_06445"
     CDS             complement(<1..825)
                     /gene="mutL"
                     /locus_tag="PKN22_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNS42381.1"
                     /translation="MKGIISVLPDHIANQIAAGEVIQRPASVVKELVENALDAGATRI
                     VVNIKDAGRTLIQITDDGKGMNATDAELCFLRHATSKIHTAADLFNLSTKGFRGEALA
                     SIAAIAHVTLQTKEENADTGTLIQIEGNKIISKEEVVCNKGTNFEVKNLFYNVPARRN
                     FLKSNDVEFRHIRDEFERIVLAHPNVNFVLIHNDTEVYNLRSAVLRKRIVDVIGDKQN
                     ERLVPIFEETSIAKITGFVIKPEYAKKTRGEQFFFVNNRFFKDAYFNNAVSKAFDNL"
     gene            complement(830..1417)
                     /locus_tag="PKN22_06450"
     CDS             complement(830..1417)
                     /locus_tag="PKN22_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNS42382.1"
                     /translation="MKYNLSEITDLIRNRRTIFPEQYSSRKVHKEQVELILNNAIWAP
                     THGKTQPWKFHVFMEDGLQRLSDFLGKTYLEITPKEEQNDMKLAKLVARPLRSSVVIA
                     VSLDRQPTSKINELEEIEAIACAIQNMSLTCTAYGLGSFWATPKLIYSDEMKNFLGLK
                     SEDKCLSLFFIGYPEGEWPKAQRRPIEYSTNWVTE"
     gene            1812..2243
                     /locus_tag="PKN22_06455"
                     /pseudo
     CDS             1812..2243
                     /locus_tag="PKN22_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072243.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
     gene            2289..3242
                     /locus_tag="PKN22_06460"
     CDS             2289..3242
                     /locus_tag="PKN22_06460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNS42383.1"
                     /translation="MSIFDKRVNYKPFEYPEVLQFVDAINKSFWVHSEIDFTADIQDF
                     KSSLTPVEKEIVKRSLLSIAQVEVGVKTFWGDLYKHFPKPEINGLGATFAECEFRHSE
                     AYSRLLEVLGYNEEFQKLLEIPVFSKKLELIENSLYSSKDIISKLLFFTIVIENASLF
                     SQFANILSFTRFKGYLKNTSNIIAWTSIDEQTHANAGIYLLNKLEEEGFVTQEIKEQM
                     AKNVLEYIQYESELLDWIYEAGELSFFSKTDMLNFMKYRVDLSLEKIGLKKLFNITDE
                     QYKPMLWFDEDVFANELDDFFAKRPTAYTKHDKSITADDLF"
     gene            3275..>4013
                     /locus_tag="PKN22_06465"
     CDS             3275..>4013
                     /locus_tag="PKN22_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNS42384.1"
                     /translation="MEQEIIEKELKKDTMERLWWKNEESEQILNRGYLLKGETVEGAI
                     DRVASAAARRLYKPELKEAFVEMIERGWMSLSSPIWANMGTERGLPISCFNVHVPDSI
                     ESITHKLGEVIMQTKTGGGTSGYFGDLRARGSAVTDNGMSSGAVSFMRLFDTAMDTIS
                     QGGVRRGAFAAYLDIDHPDCEEFLQIKDIGSPIQNLFNAVCVPDYWMQEMIDGDVEKR
                     ALWAKVLESRQQKGLPYIFFTDNVNRNK"
BASE COUNT         1275 a          659 c          804 g         1275 t
ORIGIN      
        1 caaattatca aacgctttgc tcaccgcatt gttgaaatag gcatctttaa aaaaacggtt
       61 gttcacaaag aaaaactgtt cgcctcttgt ttttttagcg tattcaggtt ttatgacaaa
      121 cccagttatt tttgcaatac tggtttcttc aaaaattggc accaagcgtt cattttgttt
      181 gtcgccaatt acatccacaa tgcgttttct caatacggca ctgcgcaagt tgtacacttc
      241 ggtgtcgtta tggattaaaa caaaattcac attgggatgt gccaaaacga tgcgttcaaa
      301 ttcatcgcgg atatggcgaa attccacatc gttggatttt aaaaaattgc gacgagcagg
      361 aacgttgtaa aacaagtttt ttacctcaaa attagttcct ttgttgcaca ccacttcttc
      421 tttcgaaatg attttgttac cttcaatttg tatgagtgtg cccgtatcgg cgttttcttc
      481 tttggtttgc aacgtaacat gggcaatagc agcaatactg gccaacgcct cgcctctaaa
      541 acctttggtg ctgaggttga ataagtctgc tgcggtatga attttactgg ttgcatggcg
      601 caaaaaacac aattcggcat cggttgcatt cattcccttg ccatcgtcgg taatttgaat
      661 gagtgtgcgc ccagcgtctt taatgtttac cacaattcgt gtagctccgg catcaagtgc
      721 attttccacc aattctttaa caacagaagc cgggcgttga atcacctctc ctgcagcaat
      781 ttgattggct atgtggtccg gaagtacaga aatgattcct ttcatacgct tattcggtaa
      841 cccagttcgt tgaatattct attggtcttc gttgagcttt tggccattca ccttcgggat
      901 aaccaatgaa aaataaactc aagcatttgt cttctgattt taaacccaag aagtttttca
      961 tttcatcgga atatataagt ttcggtgttg cccaaaagct tcccagtcca tacgcagtgc
     1021 atgttaaact catgttttga atagcacaag caatggcttc aatttcttcc aattcgttga
     1081 ttttactcgt aggttgtctg tcaagtgaaa cagcaataac aacagatgaa cgcaaagggc
     1141 gcgccaccaa ttttgccaat ttcatatcgt tttgctcttc ttttggagta atttctaaat
     1201 atgtttttcc taaaaaatca cttaaacgtt gcaagccatc ctccataaat acatgaaatt
     1261 tccacggttg tgtttttcca tgtgttggcg cccaaatagc attgtttaaa attaattcaa
     1321 cttgttcttt gtgtactttt ctagaactgt attgttctgg aaaaatggtg cgtcggttgc
     1381 gtattaaatc ggttatttca gaaagattgt atttcatttg aatattgtgt ccttttaaag
     1441 tgcaaaaata acaaatttaa agagaagatt tacgtttcac cactagaaag aaaaattgtc
     1501 aataaaaaat cattttttta gtttgaatgt tacaaataat tgtttagatt gtaaatgcta
     1561 aaattcaaat attttttatt ttttgtaaac tttttgattg ttgataaatt ttatatagct
     1621 gataatcaat gtgtaagtat aaattgtttg taaacttttt aaaaatatat ttttttcaaa
     1681 tgtttattaa tttatatttg ttcaccgaat aattgaaaat agatttctaa atcactcaaa
     1741 ttaaaatgga agcaacattg aacgaatcaa tacctgtagt tgatgcagaa aagttggagc
     1801 agcatttgcc ttttccaact acgcgaccag tggctttgga tgctgaagta gttcgttttc
     1861 aaaacaacaa agaaaaatgg attgctttta tcggtttaat tgatgggcga ccgtatgaaa
     1921 tttttacagg tcttttagac gatgaagatg gtattgcaat tccacgttgg gttgaagaag
     1981 gattgattat taaaggaaaa gaaaacgatg gttcatcgcg ctatgatttt cagtataaaa
     2041 atacgcgtgg ttacaaaaca accattgagg gactttccta caaattcaac cctgaatatt
     2101 ggaactatgc caaattgatt tcagggacct tgcgctatgg gatgcccatt gaaaaagtag
     2161 ttgatttgat caatagtttg caattagaag gaaatataaa tacttggaaa ataggggtgg
     2221 caagagcttt gaaacgctat gttccaggcg caacagatga agattaataa cattaaatag
     2281 aacaagacat gagtattttt gataaacgag tgaattacaa accttttgaa tatcctgagg
     2341 ttttacaatt tgtggatgca attaataaat cattttgggt gcacagtgag attgacttta
     2401 ctgctgatat tcaagatttt aagagcagtt taacaccagt tgaaaaagaa attgtgaaac
     2461 gttctttgtt gagcattgca caagtggaag tgggcgtaaa aacattttgg ggtgatttgt
     2521 acaagcattt tccaaaacca gaaattaacg gtttgggggc aacctttgca gaatgtgagt
     2581 tccgccactc agaagcttat tctcgtttgt tagaggtgtt ggggtacaat gaagaatttc
     2641 aaaaattatt ggaaattcct gtgtttagca aaaaattgga attaattgaa aatagtttgt
     2701 attcaagcaa agatattatt tcaaaattat tgttctttac cattgtaatt gaaaatgctt
     2761 cattgttctc gcaattcgct aatattcttt cgtttacacg ttttaaaggg tatttaaaaa
     2821 atacgtcaaa tatcattgct tggacctcta tcgacgagca aacacatgcc aatgcaggaa
     2881 tttatttgtt gaacaaattg gaagaagaag gatttgtgac gcaagaaatt aaagaacaaa
     2941 tggctaaaaa tgtattggaa tacattcaat acgagtcaga actgttggat tggatttatg
     3001 aagcaggtga attgtcattc ttttccaaaa cagacatgtt gaattttatg aaataccgcg
     3061 tggacttgtc attggagaaa atcggattga aaaaattatt caacattaca gatgaacaat
     3121 acaagccaat gctatggttt gatgaagatg tttttgcaaa tgaattggat gacttctttg
     3181 ctaaacgccc aacggcatac accaaacatg ataaaagtat aactgctgac gatttattct
     3241 aatcgtccta ataaaaccga aaaaaacaac aacaatggaa caagaaatta ttgaaaaaga
     3301 actgaaaaaa gacacaatgg aaagactgtg gtggaaaaat gaagaaagtg agcaaattct
     3361 caatcgtggt tacctattaa aaggggaaac ggtagaagga gcaattgatc gcgtggcatc
     3421 agcagctgca cgtcgcttgt ataaacctga attgaaagaa gcgtttgttg aaatgattga
     3481 acgtggatgg atgagcttga gctcgccaat ttgggccaac atgggaaccg aacgtggctt
     3541 gccaatttct tgtttcaatg tgcatgtgcc cgatagtatc gaatcaatta cgcacaaact
     3601 gggcgaagta atcatgcaaa caaaaactgg tggtggaaca tcgggttatt ttggtgattt
     3661 gcgtgccaga ggaagtgcgg taaccgacaa cggaatgagt agtggtgcag tgagctttat
     3721 gcgtttgttt gacactgcga tggataccat ttcgcaagga ggagtaagac gtggtgcttt
     3781 tgcagcttat ttggatatcg accacccaga ttgtgaagag tttttgcaaa tcaaagacat
     3841 cggtagtcct attcaaaact tgttcaatgc ggtttgtgta cccgattact ggatgcaaga
     3901 aatgattgat ggagatgtgg aaaaacgtgc tttgtgggca aaagttttgg aaagtcgcca
     3961 acaaaaagga ttgccttata ttttctttac ggataacgtc aatcgcaata aac
//
LOCUS       DAOQVQ010000182         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25403_length_3996_cov_4.085004, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000182 DAOQVQ010000000
VERSION     DAOQVQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25403_length_3996_cov_4.085004"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1297
                     /locus_tag="PKN22_06470"
     CDS             251..1297
                     /locus_tag="PKN22_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42385.1"
                     /translation="MKPLFLSIALLANGCLLAQSKVRTIDDILNQPEKKTAVYKIANS
                     YLKSAVINMSFGESSVLSVMNKTELKSADIVRIDLVFTDYPKTMDLKELNRGRIQKAL
                     EIRPDLVKDSLISWQMVRQMSCNNESEAKLLFHGVVIYYVAPQNAALSQQEQLEYQNL
                     PKDGSYKVTPKDLKSFKDSTVLAAFTRNKQWKHPTIVADVTCSMYPYISQTAFWFLLK
                     MNRKENCNIVFFNDGDAEENGIEAILEAQEKFPYSKEIIFIADNYADLRDFELISKIK
                     IPVRVILCGVEFGVNVQYINLAHKTGGSLHTMEKDLNNLVDMSEGKTFVFLGKTYIIL
                     NGEVVLKSNVIKQS"
     gene            1394..3172
                     /locus_tag="PKN22_06475"
     CDS             1394..3172
                     /locus_tag="PKN22_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNS42386.1"
                     /translation="MSNSKSLDFVQPQVLGHPSGLFVLFFTEMWERFSFYGMRVLLIQ
                     YLTAAVIAGDARSGLGWTVESASALYGTYVMLLYITPVFGGVLADKVLGSWKSVIIGA
                     IIMTIGHASMAIESNATFFIGLACLVLGTGFFKPNMPSIIGEMYKNTPEKKDGGYTIF
                     YMGVNAGAFFGMMLCGYFAERFGWHYGFGLAGIFMLLGTIQFWLAKPLFGTIGDVKKQ
                     TSEDDNEDNYKHPDEKIRNPFKKLDIILISIVSVLGLLYAFNDPISKNIPDMDMFKFL
                     DISTGNGGVFRGQFVVIIATLILFFYLLVSRIMRYGKVVRDRMFAIIILAGSLLFFFM
                     SFEQGASSLVIVARDYVDRTLVGSALTTFNIVNALLAVVPLSIISWVLIKLARVTWDK
                     ISVSNVILFVCFALIWMAVLTMLYKEFTAEASEITVSWFSTLNSFFIILLASSISKIW
                     DSKHNPAVAFKYGFGLIFVAIGFLVLAIGSWGISEGVKISMVWLVLTYLFHTIGELFI
                     SPVGLSYVSKLVPVRMLAFMYGIWYLAIAIAQKLAAVLGGQVEAIKESYSLSHFFLLF
                     TAIPLVVAIIIMLLHPVLKKLLHGVR"
     gene            3300..>3996
                     /locus_tag="PKN22_06480"
     CDS             3300..>3996
                     /locus_tag="PKN22_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNS42387.1"
                     /translation="MSTTVEKGHPKGLYLLFFTEMWERFSYYGMRAILILYLTKKLFE
                     GGLAMDPQSATLLYGYFTGLVYFTPLIGGWLADKYLGKRLSITIGGITMMIGQFTLFA
                     FTNMTGLYLGLFLLIIGNGFFKPNISTLVGGLYKDGDSRRDSAFSIFYMGINLGALIA
                     PFIIGYFTDNLFMTENADGTISYGYRYGFLASGVGMLIGQLIFNTLGQKYLGDLGKKP
                     ISSKKKVKPANITD"
BASE COUNT         1189 a          648 c          787 g         1372 t
ORIGIN      
        1 tgaaattttt gcgttgcttg atggctgtgg atatcgatga acatttgtgc ttttttcttg
       61 cctttaaaat taagaaaaac accctacttg tttaattttt aaaataaatt tttttcaaga
      121 aaataatgca atcatcctat cattgtttat atgcaatctc ttcgttttaa gattttaaca
      181 atctacttcg tttcacattt ttttgtaaca tcaagtgcta atagcgttat atttagtaaa
      241 ctctcgaatc atgaaaccct tatttctatc aattgcgctg ttggcaaacg ggtgtttgtt
      301 ggcacaaagt aaagttcgta ctatcgacga cattttaaat caaccggaga aaaaaaccgc
      361 agtttacaaa attgccaatt cctatcttaa atcggcagtt atcaacatgt cgtttggaga
      421 atcttcggtg ctatcggtta tgaacaaaac cgaactaaaa agtgcagata tagtccgcat
      481 tgatttagtt ttcacggatt atcccaaaac aatggatttg aaagaattga accgcgggcg
      541 tatccaaaaa gcattagaaa ttcgaccaga tttggtaaaa gacagtttaa tttcttggca
      601 aatggtccga cagatgtcgt gcaacaatga aagcgaagcc aaattactgt ttcatggtgt
      661 tgtaatttac tacgtggcac ctcaaaatgc agcgttgagt caacaagaac aattggaata
      721 ccaaaactta cccaaggatg gtagttacaa agtaacgcca aaagatttaa aatcatttaa
      781 agattcaacc gtattagccg cttttacacg caacaaacag tggaaacacc ctaccatagt
      841 tgccgatgtt acttgtagca tgtatcctta tatttcgcaa actgcttttt ggtttttact
      901 gaaaatgaac cgcaaagaaa attgcaacat tgtgtttttt aacgatggcg atgcagaaga
      961 aaatggtatt gaagcaatat tggaagcgca agaaaaattt ccttattcca aagaaatcat
     1021 attcattgcc gataattatg ccgatttacg ggactttgag ctcatatcaa aaataaaaat
     1081 cccagtgcgc gtgatacttt gtggcgttga atttggcgta aatgtgcaat acatcaactt
     1141 ggcgcataaa accggtggat cattacacac catggaaaaa gatttgaata atttagtaga
     1201 catgtcagaa ggaaaaacat ttgtattctt gggaaaaaca tacatcatac tcaacggaga
     1261 agtggtgcta aaaagcaatg taattaagca atcctaaagg atttttaagt tgctattgct
     1321 caaaaactgg tcatttattg aaaaaaaact gtattttcgg agattttaat tacacaaaaa
     1381 tcaataaaaa ccaatgagca atagcaaatc tcttgacttc gttcaacctc aagttttggg
     1441 tcacccaagc ggattattcg ttttgttttt caccgagatg tgggagcgtt tttcattcta
     1501 cggaatgcgt gttttgttaa tccaatacct caccgctgct gttattgcag gtgatgcaag
     1561 atctggattg ggttggacag tagaatcggc atctgccttg tatgggactt atgtaatgtt
     1621 actttacatc actccagttt ttggtggtgt tttggcagat aaagtacttg gctcgtggaa
     1681 atcggttatc attggtgcta tcatcatgac cattggacat gccagcatgg ccattgaaag
     1741 caatgctacg ttttttatag gattggcttg tttggtatta gggactggat tctttaaacc
     1801 caatatgcct tcaattattg gtgaaatgta taaaaacaca cctgaaaaga aagacggtgg
     1861 atataccatt ttttacatgg gtgtaaacgc aggtgctttc tttggaatga tgttgtgcgg
     1921 ttactttgca gagcgctttg gttggcatta tggttttgga ttggcaggta tttttatgtt
     1981 gttgggaacc atccaatttt ggttggcaaa accattgttt ggaaccattg gtgacgtaaa
     2041 aaaacaaact tcggaagatg acaacgaaga taattacaag catcccgatg aaaaaatcag
     2101 aaatccattc aaaaaattgg atattatttt aatcagcatt gtatctgttt taggattatt
     2161 atatgccttc aacgacccta tttctaaaaa tattccagac atggatatgt tcaagttttt
     2221 agatatttcc acaggtaatg gaggagtttt tagaggtcag tttgtagtca ttattgcaac
     2281 gttgatattg tttttctatt tattggtgag tcgtattatg cgctatggaa aagtagtgcg
     2341 cgatagaatg tttgccatca tcattttggc aggttcactt ctatttttct tcatgagttt
     2401 tgagcaaggt gcttcttcat tggtaattgt tgcccgcgat tatgttgacc gtactctagt
     2461 gggcagtgcc ttaacaacat ttaatatcgt aaatgcattg ttggctgttg ttccgctttc
     2521 cattatttca tgggttctta tcaaattggc acgcgtcact tgggataaaa tcagtgtttc
     2581 aaacgttatt ttattcgttt gttttgcctt gatttggatg gcggtgttaa ccatgttgta
     2641 caaagaattt acagcagagg cttctgaaat taccgtttct tggttctcaa cgctcaattc
     2701 atttttcatc attcttttag cctcttctat ctcaaaaatt tgggattcta aacacaatcc
     2761 agctgttgct ttcaaatatg gttttggatt aattttcgtt gccatcggat ttttggtgct
     2821 tgccattggt tcgtggggca tttccgaagg agtgaaaatt tcaatggttt ggttggtgct
     2881 cacctattta ttccatacca ttggggagtt gtttatttct ccggttggac tttcgtatgt
     2941 aagtaaattg gtgccggtgc gcatgttagc gtttatgtat ggaatttggt acttggccat
     3001 tgccattgca caaaaattag cagcagtatt gggcggacaa gtagaagcca tcaaagaatc
     3061 gtattcatta agtcactttt tcttattgtt tacggccatt ccgttggtgg tagcaattat
     3121 cattatgttg ttgcatccag tattgaaaaa attattacac ggagttagat aattaattgt
     3181 cagttattta atttaaacac cttgtaattt ttattcgtta ctttttaaaa aaagtagtgg
     3241 aaaagtattt tttttctaaa aattgttaac ttcacgactt tcaattttaa aacttaaaaa
     3301 tgagtacaac agtagaaaaa ggacatccaa aaggtttata tcttttgttt ttcactgaga
     3361 tgtgggaacg tttcagttat tacggaatgc gcgccattct aattctttat ttaacgaaaa
     3421 aattatttga aggagggttg gcaatggatc cgcaaagtgc aacgttgttg tacggatatt
     3481 ttacaggttt agtttacttc acgccgttaa ttggtggttg gttagccgat aaatatttag
     3541 gtaaacgctt gtcgattaca attggtggta tcaccatgat gatagggcaa tttacattat
     3601 ttgcatttac aaatatgacg gggctttacc ttggtttgtt ccttttaatt attggtaacg
     3661 gtttcttcaa accaaacatt tctacattgg ttggtggatt gtacaaagat ggcgatagca
     3721 gaagagactc ggcattctca attttctata tgggaattaa ccttggagct ttaattgcac
     3781 cgttcatcat tggttatttt acagacaact tattcatgac agaaaatgca gacggaacga
     3841 tttcttacgg ataccgttat ggattcttag cttctggtgt tggtatgttg attggtcagt
     3901 taatttttaa cacacttgga caaaaatatt taggtgattt aggtaaaaaa ccaatttcat
     3961 ctaaaaagaa agttaaaccc gctaatatta ccgata
//
LOCUS       DAOQVQ010000183         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25440_length_3991_cov_3.018039, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000183 DAOQVQ010000000
VERSION     DAOQVQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25440_length_3991_cov_3.018039"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..253)
                     /locus_tag="PKN22_06485"
     CDS             complement(14..253)
                     /locus_tag="PKN22_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42388.1"
                     /translation="MGNLTDLLRNDHHSFDLAQLDEITDGSPFELFEKWMQHAIDAKC
                     LEPNAAIIATVSSDNQPSTRICYLKELIDESLLYL"
     gene            complement(255..848)
                     /locus_tag="PKN22_06490"
     CDS             complement(255..848)
                     /locus_tag="PKN22_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HNS42389.1"
                     /translation="MNWFTVKIKFTKQLEDGTFKKVSEPYLLSALSFTDAEARIYDEL
                     GSMITGEFNVSSIARTDFHDIFAFDGAEYWWKCKIMFESGDIDAGKSKKVAQTFLVSA
                     NSAREAFENIGESLKTMLVDFEITSIMATPIVDVFPPKENLDREISRRPLEDYDMEPT
                     SPGKGVMYSASGSDIDDEELLEEEIESDIEDEYSTEE"
     gene            complement(953..2173)
                     /locus_tag="PKN22_06495"
     CDS             complement(953..2173)
                     /locus_tag="PKN22_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNS42390.1"
                     /translation="MQQTSENLMKLLSPAKLNGITLRNRIIKAATFESMLDTNNNITD
                     QCIHFHESFAKGGVGMTTLAYCAPEADGRMVDSYMYIREEIKPQLKQLAKSVHQHGAM
                     LSGQIAHCGGFSRNKKLQRKRPIAPSRVLNMVGLLYGLFFTQEMDKQLMQEVANSYAK
                     TARIMKECGFDAVELHFGHGYLLSQFISPITNKRNDEYGGSIENRMRYPIEVLNAVRQ
                     AVGPDFPILAKITMYDDLKGGISLDDGIASAKILEEAGIDGIILSAGTSSQNPMLLFH
                     GDSILPGLLHYEKNPLLKLGMKMVAKSMFRHYPYRELYLLEAAQKIRKAVKCNLIFIG
                     GANKIESLQKIMDVGFDFVQLGRPLLRDPEMVNHLHQLNENYVNGCNHCNQCAPLMND
                     PKGIRCVLPKWSNE"
     gene            2263..3135
                     /locus_tag="PKN22_06500"
     CDS             2263..3135
                     /locus_tag="PKN22_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNS42391.1"
                     /translation="MASSIFALLDDIAALMDDVAVTSKIATKQTAGILGDDLAVNAEK
                     ATGFLSSRELPVLWAITKGSFLNKLIIIPLIFVLNFFLPLAIPIILVLGGVYLAYEGV
                     EKIVEFLFHRHDKKENIIEELEKTENDPEDEKTKVKAAITTDFILSLEIVIIALSTVL
                     NKPFTIQLITVFIVSIIATVGVYGLVALIVRMDDLGYRLIRFSKGKKFFTAVGNLLVK
                     ALPLIIRSFSVIGTIALILVSGGIFVHNIHQIHEFFPTMPSLLLEVLTGLAVGLIAVL
                     VVTFVKKIITLIKK"
     gene            3199..3885
                     /locus_tag="PKN22_06505"
     CDS             3199..3885
                     /locus_tag="PKN22_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42392.1"
                     /translation="MKNIFFTSAIALLVLVSCTKETSDNINQDKIWTEYQLIYNDATH
                     ITSVKAIFKFSNALGTLLELKSPAYVLFNSDTLNYNPTMGYYEKEYPTFVNSGTFTYK
                     DLDNNVFTNAVHLADTVGFPVGLDTISKSAPFQLTWVGAAVKANETISAWLNSNVEGD
                     SRFQFTNSIGNTNLIFPLNQLTQLGVGPYGVLLMERLYSTTTMNGTSAGGVINLKYVP
                     KGLSQIEIVP"
BASE COUNT         1205 a          709 c          667 g         1410 t
ORIGIN      
        1 tgtgatgata tcttcaaagg taaagcagag attcgtcgat taattctttc aaataacaga
       61 tgcgcgtaga tggttgatta tcactgctta ccgtagcgat aattgctgca ttgggctcca
      121 aacattttgc atcaattgca tgttgcatcc atttttcaaa caattcaaac ggacttccat
      181 ctgtaatttc gtcaagttga gctaaatcaa aactatggtg atcgttgcgc aacaaatctg
      241 ttaaatttcc catgttattc ttctgttgaa tattcgtcct cgatatcgct ctctatttct
      301 tcctccaaca actcttcatc atcaatgtca gaaccagagg cactatacat cactcctttt
      361 cctggtgaag taggttccat gtcgtaatct tcaagtgggc ggcgactaat ttcacggtct
      421 aaattttctt ttggtgggaa aacatctaca attggcgttg ccataataga agtgatttca
      481 aaatcaacca acattgtttt caaggattca ccaatatttt caaaagcttc tctagcagaa
      541 tttgcagaca ctaaaaatgt ttgtgcaact ttttttgatt ttccagcatc aatatcgccc
      601 gattcaaaca tgattttgca tttccaccaa tactcagcac catcaaacgc aaaaatatcg
      661 tgaaaatctg ttcttgcaat actagataca ttaaactcac ccgttatcat tgaaccaagt
      721 tcatcataaa tacgtgcttc ggcatcagta aatgaaagtg ctgaaagtaa ataaggctct
      781 gaaacttttt tgaaagttcc atcttctaat tgttttgtaa atttaatttt tacggtaaac
      841 cagttcatac gtttgaattt aatgtaagga ttgcaaagat aatgaatttg tgttttgtat
      901 catttttttt tgaaagcttt cattggtgat tttttttaca acattcattc attcattcat
      961 tcgaccattt tggtaagaca catctaatac ctttagggtc gttcattaat ggagcacatt
     1021 gattacaatg attgcagcca tttacataat tttcatttaa ttgatgtaga tgattaacca
     1081 tttcaggatc gcggagtaat ggacgaccta attgcacaaa atcaaatccc acatccatta
     1141 ttttttgcag gctctctatt ttgtttgcac caccaataaa aataaggtta cattttacag
     1201 cttttcttat tttttgtgcc gcttctaaca ggtacaattc gcggtaagga taatgcctaa
     1261 acattgattt tgctaccatt ttcatcccaa gttttagaag tggatttttt tcataatgca
     1321 ataatcctgg taaaatggaa tcgccatgaa aaagcaacat tggattttgg ctactcgtgc
     1381 cagcactaag tataatccca tcaataccag cttcttctaa tatttttgct gatgctattc
     1441 catcatccaa acttatacct ccttttaaat catcatacat tgtaattttg gctagaatag
     1501 gaaaatcggg acctacagct tgtctgactg cattcaacac ttctatagga tagcgcattc
     1561 tgttttctat gctgccacca tactcgtcat ttcgcttatt ggttatgggg ctgatgaatt
     1621 gactcaacaa ataaccatga ccaaagtgta gctctacggc gtcaaaccca cattctttca
     1681 ttattcttgc tgtcttagcg tatgaatttg ctacttcttg catcaattgt ttatccattt
     1741 cttgggtaaa aaacaaaccg tacaataagc caaccatgtt gagtactcga gacggagcaa
     1801 taggacgttt gcgttgtaat tttttatttc tactaaaccc accacaatgt gcaatttgac
     1861 cagatagcat tgccccatgc tgatgtacac tttttgcaag ttgtttaagt tgtggtttta
     1921 tttcttcacg tatgtacata taactatcta ccatacgacc atctgcttca ggagcacaat
     1981 aagccaaagt agtcatgcca acaccacctt ttgcaaagct ttcatgaaaa tgaatgcatt
     2041 ggtcggtaat gttgttgtta gtgtccaaca tcgactcaaa ggtggctgct tttatgatac
     2101 gatttctaag tgtgatacca tttaacttag caggtgaaag aagtttcatt aagttttcag
     2161 aagtttgttg catagcattt gttttttgtt aaaaataaga ttttttctga ttttttattc
     2221 caatttatac gaatgtttat ttttgcattc acaatataaa atatggcctc aagtatattt
     2281 gcactcttag acgatattgc agcattaatg gatgatgttg ctgttacaag taaaattgct
     2341 acaaagcaaa ctgctggtat tttaggcgat gatttagctg taaatgctga aaaagcaact
     2401 ggatttttat cttctcgaga attacctgtt ttgtgggcca ttacaaaagg ctcttttttg
     2461 aataagttga ttattattcc actcattttt gtgttaaatt tcttcctgcc gttagcaatt
     2521 cctatcattt tggttttagg aggtgtttat ctggcgtatg aaggtgttga aaaaattgtt
     2581 gaatttctat ttcaccgtca cgataaaaaa gaaaatatca tcgaagagtt ggaaaaaaca
     2641 gaaaacgacc cagaagacga aaaaacaaaa gtaaaagcag ccattacaac cgattttatt
     2701 ttatcgttgg aaatcgtcat catcgcactt tctactgtgt taaataagcc ttttaccatt
     2761 caattaatta ctgtttttat tgtatccatt attgcaaccg ttggtgtgta tggattagtg
     2821 gcgttaattg ttagaatgga tgatttaggc taccgattaa ttcgtttttc gaaagggaaa
     2881 aagtttttta ccgcagttgg taatttattg gtaaaagcat tgcctctcat tattcgctct
     2941 ttttctgtaa ttggaaccat cgctttgatt ttagtttcgg gaggtatttt tgtacacaac
     3001 atccatcaaa ttcacgaatt ttttccaact atgccatcct tgttgttgga agttttaact
     3061 ggtttagctg tcgggttgat tgctgttcta gtggttactt ttgttaaaaa aataattaca
     3121 cttataaaaa aataagtttt tcttttttta tctacaagtt tcattttaca tttgttagag
     3181 ataaaatact attgacaaat gaaaaacatt ttttttactt ccgctattgc gttacttgtt
     3241 ttggttagtt gcaccaaaga aacatccgat aatattaatc aagacaaaat ctggacagaa
     3301 taccaattga tttacaatga tgcaactcac atcacatcgg tgaaagcaat ttttaaattt
     3361 agcaatgcac tcggcacttt gttggagtta aaatcaccag cttatgtact atttaatagt
     3421 gatacgttga attacaatcc aactatgggt tattatgaaa aagaatatcc aacttttgta
     3481 aatagtggta cgtttactta caaagacttg gataacaatg tgtttacaaa tgcagttcat
     3541 ttagctgata cagttggatt tccagtaggt ttagatacca ttagcaaatc agctccgttt
     3601 cagttaactt gggttggagc agcagtgaaa gcaaatgaaa ctatttctgc atggttgaat
     3661 agtaatgtgg aaggcgactc tcgctttcag tttactaatt caatcggaaa taccaattta
     3721 attttcccac ttaaccaatt aacacaattg ggtgttggac cttatggtgt tttgttgatg
     3781 gaacgtttgt atagcacaac taccatgaac gggacaagtg caggtggggt tataaaccta
     3841 aaatatgtac caaaaggatt gtcgcaaatt gaaattgtac cctaatcatc attattgatt
     3901 cacgcataaa agaagctgct ttaaatctat tttgaagcag cttctttgtt ttatgctact
     3961 gctatttgtt aagagaactt aaaaatgata c
//
LOCUS       DAOQVQ010000184         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25457_length_3989_cov_3.989578, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000184 DAOQVQ010000000
VERSION     DAOQVQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25457_length_3989_cov_3.989578"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="groL"
                     /locus_tag="PKN22_06510"
     CDS             complement(<1..1178)
                     /gene="groL"
                     /locus_tag="PKN22_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989998.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNS42393.1"
                     /translation="MAKEIYFNVEAREKLKKGVDTLSNAVKVTLGPKGRNVVIGKKYS
                     APHVTKDGVTVAKEIELKDQIENIGAQIVKEVASKTADIAGDGTTTATVLAQAIINAG
                     LKNVAAGANPMDLKRGIDKAVAEVVSQLKKMSKEVGSDNNKIKQIASISANNDEEIGS
                     LIASAMKVVGKEGVITVEEAKGTVTEVKTVEGMQFDRGYLSPYFVTNTDKMIAEMDHP
                     YILICDKKISSMKELLPVLEPVAQSGKPLLIIAEDVDGEALATLVVNKIRGAIKVAAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEERGYTLENISIEFLGTAEKVEIDKENTTIVN
                     GAGKKEEIKARVNQIKAQMEATTSDYDREKLQERLAKLAGGVAVLYVGATTEVEMKEK
                     "
     gene            complement(1206..1481)
                     /locus_tag="PKN22_06515"
     CDS             complement(1206..1481)
                     /locus_tag="PKN22_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNS42394.1"
                     /translation="MAISFKPLADRVLVEPTPAEVKTASGIFIPDTAKEKPVKGTIVA
                     AGKGKKDEPMTVKVGDEVLYGQYSGTEIKLDGKTYLIMREADIYGIL"
     gene            1693..>3989
                     /locus_tag="PKN22_06520"
     CDS             1693..>3989
                     /locus_tag="PKN22_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42395.1"
                     /translation="MIKYFTLLLTGLLLTCNAVFAQKLKHEQVSPFFFGLNAGATWQS
                     TDVKTKVDLGAGFVFGASFNRDYGNIFSYDLKFRFLGGSWYGQNIRKTDFSDYDNTTL
                     STVPTDYKSTLGYSINNFKSKSREFNLELSIHLNRLTERTGWDPYIFGGIGATYFRTY
                     GNLLDKDGFTYNYDQQESYSKTSLSQLMDKTYETGLDGSTTFGNLEMMGHLGFGIGYY
                     FRPNIAVGFEHKTTFTGGDRFDGVFANKGILQKDIYHYSGLYLKIYFKRGRYKQENNK
                     PTTPQEPVVTPSNPTNPTNPTNPTPPVDCPTPVLSVQTTPATVNQASFQVVGTVLNNS
                     SSGVEATLNGVNTGAIDYFGASNFTATFQLQPGLNTIVLTAINDCGRDSKTIQVNYEA
                     CKTPVVTFVQPTTSNSISVNPTVAITANIADLGNGNIQYVVNNQASTNFSFNASTQQL
                     SSVVNLQNGNNTIHITATNACGSNSQTIYVQYNPVVQCDKPVINIQAPTNYSGNVSTI
                     ELAALITNISSAAQVDVYVNGTKQSAGTYISRLKGFSKIINLNNGRNQILIRATNDCG
                     STEQSFIYDYNLPCNEPVVNLTTPSGSRVTSLVSTYVVSGHISNLKSVQNIVFKVNGV
                     AISAYNFSLQTGLFSATVTLKAGENTIELSATNECGKASANSVIVYNQPCDKPVVTFI
                     KPLTSTISVSKGDYTIEASILNIHTANEVVLTVNGVVQSVGTYNSTTRVYSKTVQLNE
                     GSNLIQIAAKNSCGEAVSSATISYK"
BASE COUNT         1191 a          785 c          665 g         1348 t
ORIGIN      
        1 tttttctctt tcatttcaac ttctgtagtt gcaccaacat aaagaacagc aacacctcca
       61 gccaatttag ctaaacgttc ttgcaatttt tcacggtcgt aatcagaagt tgttgcttcc
      121 atttgtgctt taatttggtt tacacgcgct ttaatttctt ctttcttgcc cgcaccgttt
      181 acgattgtgg tgttttcttt atcaatttca actttttcag cagttcctaa aaactcaatt
      241 gaaatgtttt ctaatgtata tcctctttct tcagaaatta cttgtccacc agtcaaaatt
      301 gcgatgtctt ccaacatagc ttttcttctg tcgccaaaac ctggagcttt aacagctgcc
      361 actttgattg ctcctctgat tttatttaca accaatgtag ccaacgcttc tccatccaca
      421 tcttcagcaa tgattaacaa cggttttccc gattgagcaa ctggttccaa tacaggaagc
      481 aattccttca tgcttgaaat ctttttgtcg caaatcaaaa tgtaagggtg atccatttct
      541 gcaatcattt tgtcggtatt ggtaacaaaa taaggagaaa gataacctct gtcaaattgc
      601 ataccttcta cagttttaac ttctgtaaca gtaccttttg cttcttcaac agtaatcact
      661 ccttctttac caacaacttt cattgcagat gcaatcaacg aaccaatttc ttcatcgttg
      721 tttgctgaaa tagaagcaat ttgcttgatt ttgttgttgt cagaaccaac ttcttttgac
      781 atttttttca attggctaac aacctctgca actgccttgt cgattccacg ttttaaatcc
      841 attggattag caccagcagc aacgtttttc aatccagcat tgataattgc ttgcgctaaa
      901 acggttgccg ttgttgttcc atcaccagca atatccgctg tttttgaagc aacttctttt
      961 acgatttgtg caccaatgtt ttcaatttgg tcttttaatt caatttcttt tgcaacagtt
     1021 acaccgtctt ttgttacatg aggtgcactg tattttttac cgataacaac gtttctacct
     1081 tttggtccca acgttacttt tactgcattg gataatgtgt caactccttt ttttaatttt
     1141 tcgcgagctt caacattgaa ataaatttct tttgccattt tgtattcgat tttttaattg
     1201 tctgattata aaattccgta aatatctgct tcacgcataa ttaaataggt ttttccgtcc
     1261 aatttaattt cagttcctga atattgtccg tataaaactt catcaccaac ttttacggtc
     1321 attggctcgt cttttttccc ttttcctgct gcaacaattg ttccttttac aggtttttct
     1381 tttgctgtat cagggatgaa aattccactt gctgttttaa cttctgccgg tgtaggttca
     1441 accaaaacac ggtcagctaa aggtttaaat gatattgcca taattcttct attttttgtt
     1501 acgcctatct ttcgaccaat tttatgccat tcatccaaaa tgcaattttt gacagaataa
     1561 acatttttta aactgacata ctgacacttt taacgtttcc aatcagatga atgcactcca
     1621 attcaatttt tttttccgaa ttttggcacg aaaaatgaaa agtcgctcat aagacaccct
     1681 aaattataca gtatgataaa atattttaca cttcttttaa ctggtttatt gctaacatgt
     1741 aacgcagttt ttgcacaaaa attaaagcat gaacaagttt cgccattttt ctttgggctg
     1801 aatgccggag caacttggca aagtacagat gtaaaaacaa aagttgactt gggtgcaggt
     1861 tttgtttttg gagcttcttt taaccgcgat tatggcaata tttttagcta cgaccttaaa
     1921 tttcgatttt tgggtggtag ctggtatgga caaaacattc gtaagactga ttttagcgat
     1981 tatgataata caactttgag cactgttcca acagattata aatcaacgtt gggttattct
     2041 attaacaact ttaaaagtaa gtcaagagaa tttaatttag aactttccat tcacttgaat
     2101 cgtttgaccg agcgtacagg ctgggatccc tacatttttg gtggaatagg agcaacttat
     2161 tttagaacct atggaaattt attggacaag gatggattta cctacaatta cgatcagcaa
     2221 gagagctatt ccaaaacatc gttgagtcaa ttgatggata aaacctatga aacagggttg
     2281 gatggaagta caacttttgg aaaccttgaa atgatggggc acttaggttt tggaattggc
     2341 tattatttcc gccccaatat tgctgttgga tttgaacaca aaacaacatt tacaggtggc
     2401 gatcgctttg atggtgtttt tgctaataag ggaattcttc aaaaagacat ttaccattat
     2461 tcgggcttgt atttgaaaat ttacttcaaa cgaggaagat acaaacaaga aaacaataaa
     2521 ccaacaacac cacaagaacc tgttgttaca ccgtccaatc caacaaatcc aacaaacccg
     2581 accaatccta caccaccagt tgattgtccg acacctgttt tgagtgttca aactacacct
     2641 gcaacagtca atcaggcaag ttttcaagta gttggaacag ttttgaataa ttcgagtagt
     2701 ggagtagaag ctacattgaa cggagttaac actggagcaa tcgattattt tggagcgtca
     2761 aattttacag ctacttttca acttcaaccg ggattgaaca ccatagtttt aacggcaatt
     2821 aacgattgtg gaagagattc taaaaccatt caagttaatt atgaagcatg caaaacccct
     2881 gtggtaacgt ttgtgcaacc aactacttct aattctattt ctgtaaatcc aacagttgca
     2941 ataactgcca atattgccga tttaggaaat ggaaacattc agtatgtggt taacaatcaa
     3001 gcttctacta attttagttt caatgcatct acacaacaat tgtcgagtgt ggttaatttg
     3061 caaaatggca ataataccat tcacattacg gcaaccaatg cctgtggttc caactcacaa
     3121 actatttatg tgcagtacaa tccggtggtg caatgcgaca aaccagtcat taacattcag
     3181 gcaccaacca attatagtgg caatgtgagc accattgaat tagctgcttt gattaccaat
     3241 atttcaagtg ctgcgcaagt tgatgtgtat gtaaatggaa ccaaacaatc ggctgggact
     3301 tatatttcac gtttgaaagg tttttctaaa atcattaatt taaacaacgg acgcaatcaa
     3361 attctgattc gcgcaaccaa tgattgtggc tcaaccgaac agtcgtttat ttatgattac
     3421 aatttgccgt gcaatgaacc agttgttaac ttgacaactc cttcgggatc tagagtaact
     3481 tcgcttgttt caacctatgt ggtgagtggt cacatttcta atcttaaatc agttcaaaat
     3541 attgtgttta aggtgaatgg cgttgcaatt tctgcctata attttagttt gcaaacagga
     3601 ttgttttcag caactgttac attgaaagct ggagaaaaca ccatcgaatt gagcgcaacc
     3661 aacgaatgtg gtaaagcaag tgcaaattct gtaattgttt acaatcaacc atgtgataag
     3721 ccagttgtta ctttcattaa accattgact tcaactattt ctgtttcaaa aggagattat
     3781 accatcgaag catctatttt gaacattcat acagcaaacg aagttgtttt aacggtgaat
     3841 ggtgttgtgc aatctgttgg aacgtataac agcactacac gtgtatatag caaaaccgtt
     3901 caattgaatg aaggctcaaa tttgatacaa atcgctgcaa aaaacagttg tggagaagct
     3961 gtttcaagtg ccactatttc ctataaaaa
//
LOCUS       DAOQVQ010000185         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25459_length_3989_cov_3.390442, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000185 DAOQVQ010000000
VERSION     DAOQVQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25459_length_3989_cov_3.390442"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKN22_06525"
     CDS             complement(<1..785)
                     /locus_tag="PKN22_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNS42396.1"
                     /translation="MEVQNELFQHLISFAASTEYGKKYQFDSISSYADFKEKVPLTNY
                     DDLKPYIDRVMEGEQGLLWPTSTKWFAKSSGTTSTKSKLIPVTRESLEDCHYKGGKDL
                     LSIYYNNYENRKLYNGKHLIVGGSAQVNHLSDDSYFGDLSAIIVKNLPWWAEIRRTPA
                     KEITLMNEWEEKLEKMAHSTLDEDVYIIAGVPSWTMVLIKRVLQLSGKKTLKEVWPNL
                     ELYMHGGVSFEPYRHEFEKLIGFDDMHYVETYNASEGFFGIQD"
     gene            complement(1196..1534)
                     /locus_tag="PKN22_06530"
     CDS             complement(1196..1534)
                     /locus_tag="PKN22_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42397.1"
                     /translation="MKSIKFISGLSFSIAILLMILMLLELPFTVNAQLNLSYASMAFG
                     FIGIVLNLINVKDSKHRVSFSIVFWIATALLTVGIWWWAIDSTSVLAKIILFTGIASM
                     LIALALPKKR"
     gene            complement(1538..2599)
                     /locus_tag="PKN22_06535"
     CDS             complement(1538..2599)
                     /locus_tag="PKN22_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42398.1"
                     /translation="MKRAVLFLVILFFGVFTSVWTQDLAQQEQELAELLSNLRAAKNN
                     AEKQKANTVFKAKMEATIQLKGAFEYPFSQLKTVGTIKSPDNVFRFFNWNVEQDDMTQ
                     KYYCYILKFNDRTEQYDVIELKDKSFMLPRRPQDILESDMWYGALYYKIIPFKKGSKE
                     VYTILGWMGNNSSSTMKVIDVLSFSGKVARLGSPVFKNGKELQKRVFFEFAEKTSMYL
                     NYEEKYNRIIFDHLSPETPELKGMYSMYLPDLSYDAYQFENGKWVLKEDVIGVNNKEA
                     DAIKVQVKDEKTGKIKEIQVKNKWEGPSTSHVATTPEEQMKGVENKENTPKTKEKKQK
                     GKTPQSYNPVDNIKHKKKR"
     gene            complement(2631..2960)
                     /locus_tag="PKN22_06540"
     CDS             complement(2631..2960)
                     /locus_tag="PKN22_06540"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNS42399.1"
                     /translation="MSKELTSEFGKGFGERVVFLMRQFYLTFPDFQIVRTLCAQLTWS
                     HFKRVLSVTNEKAQMYYLQEAAAQTWSIRTLDRNISTLYFNKILHLVIKSWRNFCSAL
                     KNWLVVV"
     gene            complement(3069..>3989)
                     /locus_tag="PKN22_06545"
     CDS             complement(3069..>3989)
                     /locus_tag="PKN22_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNS42400.1"
                     /translation="GRFGNEVEEMFQNTDFSNIQKILYRPFEERFTYYTGTTKGFHCM
                     PRSETMQHLFKQNNVALICPKQVPDKEESGVFITKYIAGHKTSSAYNINNVFPLYLYP
                     EPTLGAESERVPNLNMEIVEKIEKDLNHSFLHDDNIVCDLAAGWDGTFSPLDILDYIY
                     AVLHSPTYRETYKEFLKIDFPRVPYPKNQESFWKLVELGAEIRQIHLLESSKVEKYIT
                     QYPVDGDNVVGKPKYENGKVFINETQYFDNVPQVAWEFYIGGYQPAQKWLKDRKDRTL
                     DFEDILHYQKIIVALTETDRLMKEIDKIIL"
BASE COUNT         1184 a          750 c          641 g         1414 t
ORIGIN      
        1 ttatcttgaa taccaaaaaa accttcggat gcgttgtagg tttctacata atgcatgtcg
       61 tcaaagccaa ttagtttctc aaattcatga cgatagggtt caaaactcac gcctccgtgc
      121 atgtacaatt ccaggttggg ccaaacttct ttcaacgttt ttttacccga tagttgaagc
      181 acccgtttta tcaaaaccat ggtccaactt ggcactcctg caataatata cacatcttca
      241 tctagggttg aatgggccat tttttctaat ttttcttccc attcgttcat caatgttatt
      301 tctttggctg gtgtacgacg aatttctgcc caccaaggta aatttttcac aatgatggca
      361 ctcaaatcac caaaatacga atcatccgaa agatggttaa cttgtgcact tccgccaaca
      421 attaaatgtt ttccgttgta taatttccga ttttcgtagt tgttgtaata aatagacaac
      481 aaatctttac cacctttgta gtggcaatct tccaaagatt cacgggttac gggaatcaac
      541 ttactttttg tagaggtggt gccacttgat tttgcaaacc atttagtaga tgttggccac
      601 aacaagcctt gctcgccctc catcacacgg tcgatataag gttttaaatc gtcgtaattg
      661 gtgagcggaa ctttttcttt aaaatcagca taggaagaga tgctatcaaa ctgatatttt
      721 tttccatatt ctgttgatgc tgcaaaagaa ataaggtgtt gaaaaagctc gttttgaact
      781 tccaacggaa attttttaaa aagctctatt tggtgaatac gcttttttac aatccaagaa
      841 aatattgaat taaacggcat aaagttagtc taatttgttg caatatgttt aactaatgta
      901 ataaaaatat tacaaacgcc aacaatttag acaaaaacaa actatttatt gattaaccga
      961 agaatacgat gtatcctaca attgctaaac aaatgattgt ataaatagtt cctaaagcta
     1021 ctgttgatcc ttcgaagaaa ttatctttat ttgccatttt gaatattatt ttttttcaaa
     1081 agtagtgttt taattgttat ttgcaaccat tgaaatgact ttttttggtt ttaaaaacaa
     1141 acattgaatt gttgaaattc gttctatcca ttcaaacgaa ttggatttct gttatttatc
     1201 gttttttagg taatgccaaa gcgataagca tgctggcaat tccagtgaac aaaataattt
     1261 ttgcaagaac agaggtagaa tcaattgccc accaccaaat accaacagta agcaatgctg
     1321 ttgctatcca aaatacgatg ctaaaactca cgcggtgctt gctatctttt acgttgataa
     1381 ggtttaaaac aataccaata aatccaaaag ccattgaagc ataagacaga ttaagttgcg
     1441 cattaaccgt aaaaggcaat tctaaaagca ttaaaatcat cagcaagatg gcgatgctaa
     1501 acgataaacc cgaaatgaat tttatggatt tcattgccta tctttttttc ttgtgcttaa
     1561 tgttgtcaac aggattgtag ctttgagggg tttttccttt ttgctttttt tcttttgttt
     1621 ttggcgtatt ttctttgttt tcaacgcctt tcatctgttc ttcaggagtt gtagcaacat
     1681 gcgatgtgga tggtccttcc cacttgtttt taacttgaat ttcttttatt ttccctgttt
     1741 tttcgtcttt cacctgcact tttatggcat ctgcttcttt gttgttaacg ccaattacgt
     1801 cttctttcaa aacccattta ccgttttcaa attgataggc atcatacgat aaatcgggca
     1861 aatacataga atacattcct tttaattcgg gtgtttcggg cgataggtga tcaaaaataa
     1921 tgcggttgta tttttcttcg taattcaaat acatggatgt tttttcggca aattcaaaaa
     1981 atacacgttt ttgcagttct ttgccatttt taaaaaccgg actacccaag cgtgccactt
     2041 ttccgctaaa agaaagtacg tcaataactt tcatggtgct ggatgaattg ttgcccatcc
     2101 atcccaaaat ggtgtacacc tcttttgaac cttttttgaa cgggatgatt ttatagtaca
     2161 aagcaccata ccacatatcg ctttctaaaa tgtcttgcgg tcgacgtggc aacataaaac
     2221 ttttatcttt cagctcaata acatcgtatt gctcggttct atcattgaat tttagaatgt
     2281 agcaatagta tttttgggtc atatcgtctt gctccacatt ccaattaaag aaacgaaaaa
     2341 cattatcggg gcttttgatg gtacccactg tttttaactg agaaaaagga tattcaaaag
     2401 caccttttaa ttggatggtt gcttccatct ttgctttaaa aactgtattt gctttttgtt
     2461 tttcagcatt attttttgct gcacgcaggt tggataataa ctcagccaac tcttgttctt
     2521 gttgtgctaa atcttgcgtc cacacagagg tgaacactcc aaaaaataaa ataaccaaaa
     2581 ataaaacagc tcttttcata cttctttaaa acggatagaa cggtgaattg ttacacaact
     2641 accaaccaat tttttagagc agaacagaaa ttacgccaac tttttattac cagatgcaat
     2701 attttgttga aatataaggt agaaatattt ctatccaatg ttctaataga ccaagtttgt
     2761 gcggcagctt cttgcaagta atacatttga gctttttcgt ttgtaacgct taaaactctt
     2821 ttaaaatgtg accatgtcaa ttgtgcacac agtgttcgca caatttgaaa atctggaaag
     2881 gttaaataaa actgtctcat taaaaagaca actctctctc caaatccttt cccaaattct
     2941 gaggttagtt cttttgaaat tgattaaatc aattcttttc catattctgc tctgtctttt
     3001 ccgttttgtt cttctagcac aatgcgttca ctaattttcc aataagcttc aaccattgca
     3061 gtgtaaatct acaatatgat tttatcaatc tccttcatca atctatcggt ttctgtcaat
     3121 gccacaataa ttttttgata atgcaaaata tcttcaaaat ctaaagttcg gtctttgcgg
     3181 tctttaagcc atttttgtgc cggttggtag ccaccaatgt aaaactccca agccacttgc
     3241 ggaacattgt caaaatattg ggtttcgttg ataaatactt ttccgttttc gtattttggt
     3301 ttaccaacta cattgtctcc atcaactgga tattgagtaa tatatttttc aacttttgag
     3361 ctttccagca aatgaatttg gcgtatttct gcacctaact ccaccaattt ccaaaacgat
     3421 tcttggtttt ttggataagg cactctcgga aaatctattt tcagaaattc tttgtaggtt
     3481 tctctgtaag ttggcgaatg taaaacggca tagatatagt ctaaaatatc taacggagag
     3541 aatgtgccat cccaaccagc tgctaggtca catacgatat tatcatcatg gagaaaacta
     3601 tgattgaggt ctttttctat tttctccaca atctccatat tcaaattagg aactcgctcc
     3661 gactctgctc ctaatgttgg ctcgggataa aggtagagag ggaatacatt gtttatgttg
     3721 tatgcactac ttgttttgtg tcctgcaata tattttgtga taaatactcc agattcttct
     3781 ttgtcaggaa cttgttttgg acaaattaat gcaacgttat tttgtttaaa tagatgttgc
     3841 attgtttcac ttcgaggcat acaatgaaac ccttttgttg tccctgtata ataagtaaat
     3901 ctttcttcaa atggacgata aagtattttt tggatatttg aaaaatcagt attttggaac
     3961 atttcttcaa cttcattccc aaagcgccc
//
LOCUS       DAOQVQ010000186         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25653_length_3969_cov_3.267757, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000186 DAOQVQ010000000
VERSION     DAOQVQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25653_length_3969_cov_3.267757"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..2226)
                     /locus_tag="PKN22_06550"
     CDS             complement(85..2226)
                     /locus_tag="PKN22_06550"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNS42401.1"
                     /translation="MNKLKVYFLTFLVSFSFLFARADEGMLLPLLIGRSYEQMKKYGL
                     NLTQEEIYSINHSSLKDAIISFNGYCTGEVISKNGLILTNHHCGYDAIAAASTPEHNY
                     LDNGFWAKNHQEEIKTDLFATFIVRIEDVTNKVNTVLNANMTEAERAAKAQEVGKIIA
                     KEATNGTHYEAFVRDFYDGNEYYLFVTEKFTDIRFVGTPPQSAGKFGGDTDNWMWPRH
                     TADFSIFRIYSGRDGKPAPYSADNVPYSPKHHLPISLKGVKENDFAMIMGFPGRTDRY
                     LSSWGVEQTVSLEKPKRVELRAAKMDVMKRYMDQDVAVRLKYSSEYASVANYWKNFQG
                     EILQVKNNKVVAKKQAIEKQFEAFANDKDEYKNVLSTFASSFKTLESVVLIKAYQSEF
                     VFSVNAALLAYRYKMYKDAANSGNKERADMILAFTNNAANEYFANSSMAIESDIVAEI
                     LKQYVKDIPASQQGEFTKKIAAKGEKGINSFVKTMKAKSIFFNKALFDKFVANPSIKT
                     LDKDPLFKLIADISAAYEKALATPEIQKATDDLKKANRDFTRGVRLMMPNKEFYPNAN
                     STLRLTYGQVLPYSPKDAVYYDYVTSLDGMIQKEDLTNPEFHIDPRIKEVYAKKDWGQ
                     YADKERGYVVANFLSNNDITGGNSGSPVINADGHLIGTAFDGNWEAMSGNIFFEEKVQ
                     RTISCDIRYILWLVDRVYGAQNLIDEMTLVK"
     gene            2516..3364
                     /locus_tag="PKN22_06555"
     CDS             2516..3364
                     /locus_tag="PKN22_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42402.1"
                     /translation="MSNSFAQVLNNSKGEAFENKPFFNQTFIAKNKIKSIKGQFSYKK
                     SGKAMLTTNYYYAYNFNPKGELESTFETKTDDGTVDTTWNVYLYNELGLLAEHKQGTR
                     QAKTGVVYQYDSKNRVVSEDYSTESIDSTGQKVMLLINTETMSYEDYGKQVKKTILNS
                     YGLPYITEMSYYDSNGYLLEVEEKYIRTSNFIKKLYSYNDSGLLVGIKIFHKGHEAPV
                     EEMTFKYDDFGNLLEKHFFKHGEFITDTQVVYNEKSKLMTAVIIRDVPTNFIMVIRYG
                     DYEFYE"
     gene            complement(3355..3717)
                     /locus_tag="PKN22_06560"
     CDS             complement(3355..3717)
                     /locus_tag="PKN22_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase"
                     /protein_id="HNS42403.1"
                     /translation="MLLLDSLYRVDALRMEENQLIANIHLNENDAIFKGHFPGNPITP
                     GVAQLEIIKELLAMHYQRSIQLKSISNCKYLAIMNPIEHPQIEVKITISESEEGIKTN
                     TVFSAGDKTFTKSAGIYS"
     gene            complement(3717..>3969)
                     /locus_tag="PKN22_06565"
     CDS             complement(3717..>3969)
                     /locus_tag="PKN22_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNS42404.1"
                     /translation="GDFLNQKDFSISYEENSKLYRLTLSPKSPRMTKYIAKIELLFSR
                     SSLLLEEMTFFEKDSQKVHYTFSDIETNVKIDDSKFNQK"
BASE COUNT         1280 a          668 c          626 g         1395 t
ORIGIN      
        1 ccttttttca tgtcctataa attctgcggc acaaaaaaac tgcaccaatt tcttgatgca
       61 gtttttgaat gaattcctta tttattattt caccaatgtc atttcgtcaa ttaggttttg
      121 tgcaccatac acacggtcaa ctaaccataa aatgtaacga atatcgcatg aaattgtacg
      181 ttgaactttc tcttcaaaga aaatatttcc tgacattgct tcccagtttc catcaaatgc
      241 agttccaatc aaatgaccat ctgcgttgat aactggagaa ccagagttac ctcctgtaat
      301 gtcgttgtta ctcaaaaagt tggcaactac ataacctctt tctttgtcgg catattgtcc
      361 ccaatctttt ttagcatata cttctttgat gcgagggtca atgtgaaact caggatttgt
      421 taaatcttct ttttgaatca ttccgtccaa acttgttaca tagtcataat aaaccgcatc
      481 ttttggagaa tagggtaaaa cttgaccgta agttaaacgc aacgtagagt tagcatttgg
      541 atagaattct ttattcggca tcatcaaacg aacacctcta gtaaaatctc tgtttgcttt
      601 tttcaaatca tcagttgctt tttgaatttc tggagttgct aatgcttttt cataagccgc
      661 agaaatgtca gcaattaact taaacaaagg atctttatcc agcgttttta tagaaggatt
      721 tgctacaaat ttgtcaaaca acgctttatt aaagaaaata gattttgctt tcatagtttt
      781 tacaaaacta ttgatgcctt tttctccttt agcagcaatt tttttagtga attcaccttg
      841 ctgacttgca ggaatatctt tcacatattg ttttaagatt tcagcaacaa tatccgattc
      901 aattgccata gaagagttcg caaaatattc atttgcagca ttgtttgtaa atgctaaaat
      961 catatccgct ctttctttgt tacccgaatt agcagcatct ttatacattt tatagcggta
     1021 tgccaataac gcagcattaa cagaaaatac aaattctgat tgatatgctt tgattagcac
     1081 cacactttcc aacgttttaa agctagatgc aaatgtactc aatacgtttt tgtattcatc
     1141 tttgtcattt gcaaatgctt caaattgttt ttcaatggct tgtttttttg caactacttt
     1201 gttgttttta acttgcaaaa tttctccttg gaagtttttc caatagttag caactgatgc
     1261 atattcagaa gaatatttca aacgaacagc aacatcttga tccatgtagc gtttcattac
     1321 gtccattttt gctgcacgca attcaacgcg tttaggtttt tctaagctta cagtttgctc
     1381 aacaccccaa gatgataaat aacgatctgt tcttcctggg aatcccataa tcattgcaaa
     1441 atcattttct tttactcctt ttaacgaaat aggtaagtgg tgtttaggag aataaggaac
     1501 gttgtcagca gaataaggtg ctggttttcc atctcttcca gaataaattc taaaaattga
     1561 aaagtcagca gtgtgacgag gccacatcca gttgtctgta tcgccaccaa atttacctgc
     1621 tgattgtgga ggagttccta caaaacgaat gtctgtaaat ttctctgtaa caaataaata
     1681 atattcgttg ccgtcataaa aatctctcac aaatgcttcg taatgtgtac catttgtagc
     1741 ttcttttgca ataattttac caacctcttg cgcttttgca gctctttcag cttcggtcat
     1801 attagcattc aaaacagtat ttactttatt ggtaacatct tcaattctta caataaatgt
     1861 tgcaaacaaa tccgttttga tttcttcttg atgattttta gcccaaaatc cattatctaa
     1921 ataattgtgc tctggagttg atgcagcagc aattgcatca taaccacagt ggtgattcgt
     1981 taaaatcaaa ccatttttag aaataacttc acctgtacaa taaccattaa aagaaataat
     2041 agcatctttt aatgatgaat ggttgatgga gtaaatttct tcttgagtta agtttaaccc
     2101 atattttttc atttgctcat aacttcttcc aatcaataaa ggaagtaaca ttccttcgtc
     2161 ggcacgtgca aacaaaaacg aaaagctaac taaaaacgtt agaaaataga cttttaattt
     2221 attcataaca gatctaaatt ttattttgga tagcaaaaat aggaatatta aaccgtaaca
     2281 aaaattattt ttcacactca atgcgtgtaa tttttcaaaa ttctttaaac gcatgttaat
     2341 aattgtacaa aatattcaat tcaactactt gtaaatgaac taaaacgatt tttacttcgt
     2401 tttgtttatt aaaaaaatgg tgttattaat tatttagcat tgtttttgaa tgcgtcaatt
     2461 gttttgtcat ttttgtaatg tgagaaatag tgtaattttt attttgtgtc tgattttgag
     2521 taattctttt gctcaagtgt tgaataattc aaaaggggag gcttttgaaa acaaaccgtt
     2581 ttttaaccaa acttttatag caaaaaataa aattaagtcc atcaaaggac agtttagcta
     2641 taaaaaatcg ggtaaagcaa tgttgacaac aaactattat tatgcttata attttaaccc
     2701 aaaaggtgag ttagagagca cgtttgaaac caaaacagat gatggaacgg tagatacaac
     2761 ttggaatgtt tatctctaca atgaattagg attgttggca gaacataaac aaggtacacg
     2821 ccaagccaaa acaggggttg tttatcaata cgattcaaaa aatagagtag tatctgaaga
     2881 ttattctacc gagtcaatag attcaacagg gcaaaaagta atgttactca tcaacaccga
     2941 aaccatgtcg tatgaagatt atgggaaaca agtgaaaaag acgattttaa acagctatgg
     3001 attgccttat attactgaaa tgtcttatta tgacagcaat ggctatttgt tagaggtgga
     3061 agagaaatac attcgtacat ccaattttat taaaaaatta tattcttaca acgatagtgg
     3121 cttgttggtt ggaattaaaa tttttcacaa aggacatgaa gctccagttg aagaaatgac
     3181 ctttaaatac gatgattttg gaaatttatt ggaaaaacat ttctttaaac acggtgagtt
     3241 tattaccgat acccaagtag tttacaacga gaaatcaaag ttgatgactg ctgtaatcat
     3301 ccgtgatgtg ccaactaatt ttattatggt tattcgctat ggtgactacg agttttacga
     3361 ataaatacca gcgcttttag taaaagtttt atcgcctgca ctaaaaacag tgttggtttt
     3421 tatgccttct tcgctttctg aaatggtaat ctttacctct atttgtgggt gttctatcgg
     3481 attcattatt gctaaatact tgcaattaga aatgcttttc aattgaatag aacgttggta
     3541 atgcattgcc aataattctt taatgatttc taattgtgca acacctggtg taattgggtt
     3601 tccgggaaaa tgacctttaa aaatagcatc gttttcattc aaatgaatgt ttgcaattag
     3661 ttgattttcc tccattcgaa gcgcatcaac acggtataat gaatcaagta acagcattat
     3721 ttttggttaa atttagaatc gtctattttt acatttgttt caatgtccga aaaagtatag
     3781 tgtacttttt gactgtcttt ttcaaaaaac gtcatttctt ctaataaaag tgaagaacga
     3841 ctaaacaata attcaatttt agcaatgtat ttagtcattc gcggactttt aggtgataac
     3901 gttaaacgat aaagcttgga gttttcttca tagctgatgg aaaaatcttt ttggtttaga
     3961 aaatcgccc
//
LOCUS       DAOQVQ010000187         3960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25745_length_3960_cov_4.075544, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000187 DAOQVQ010000000
VERSION     DAOQVQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25745_length_3960_cov_4.075544"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /locus_tag="PKN22_06570"
     CDS             <1..1822
                     /locus_tag="PKN22_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNS42405.1"
                     /translation="ENDEILAEDRMSRRPELGRIFKAYSDRCFKAGAMDFDDLLYQMY
                     VLLRDFPDVLGYYQQKFQYILVDEYQDTNYAQYTIVKMLAAAYENICVVGDDAQSIYS
                     FRGANIQNIFNFRADYPDFKLFKLEQNYRSTRNIVEAANSVIARNVDQIKKEVWTENG
                     EGALLNVIRTLTDNEEGKTVVNRLFDIKTQEGIEFSDFAILYRTNRQSRSFEEALRKM
                     NIPYKIYGGISFYQRKEIKDMLAYFRLTANQHDEEAFKRVINYPKRGIGKTTIENITI
                     ASTNYGVSIWDVVTNLDQYPVQMNSGTRTKINEFTTMILSFAAQLEKSNAYELGQHIA
                     KSSGILKELYDEKEKGPEEVERYQNIEELISGLKEFTVSKAETDEFASLSEFMMDVAL
                     LTDADNDNSEDKNYVTLMTIHSSKGLEFPHVFLVGLEENLFPSQMALQSRTETEEERR
                     LFYVAVTRAMKSCTLTYASSRFQWGNLTSSEPSRFIDEIDPKYLHFEAPQRGQGRSLS
                     SGITSNRSMGGLNKTFDAPRPAVAATPRNLKPIATVRGGASDSLEYKVGYNVLHERFG
                     KGKITKIEGQGADKKATIFFPHHGAKTLLLRFANLTVVEA"
     gene            complement(1824..3143)
                     /gene="mtaB"
                     /locus_tag="PKN22_06575"
     CDS             complement(1824..3143)
                     /gene="mtaB"
                     /locus_tag="PKN22_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNS42406.1"
                     /translation="MIQFKKVAFYTLGCKLNFAETSTIARLFENAGFAKVEFEDTPDI
                     YVINTCSVTEKADSKCKQIVKKCLKVNPNAFIVVVGCYAQLKPEEIAKIWGVDLVLGA
                     NEKFNILDYVDETLEKKDKALVSFENIKKTKEFVPAFSFGDRTRTFLKIQDGCDYFCT
                     FCTIPLARGKSRNASIAETVVQAQKAVDEGAKEIVLAGVNIGDFGQGGEENFYGLIQE
                     LDKVKGVERIRISSIEPNLLSNEIIHFCLENSNHFVPHFHIPLQSGSNKQLQKMRRKY
                     LRELFAERIETIKNHNADCCIGVDVIVGFPGETDEDFEDTVNFIKDLTVSYLHVFTYS
                     ERANTTAIKMGDSVPMALRHERSKRLHILSDKKKRAFYEENVGKTKAVLFENDNEDGF
                     MHGFTDNYVKVKFPYNAQLTNTVHMVQLTEIDRDGVMKCEMVEKVEA"
     gene            complement(3227..>3960)
                     /locus_tag="PKN22_06580"
     CDS             complement(3227..>3960)
                     /locus_tag="PKN22_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS42407.1"
                     /translation="FAYSINVILKQKRLSDIKNDFINNMTHELKTPISTIGLSSEMLL
                     KGTITEKEDIQRYAGVIFKENNRLKNQVERVLNIAKLDKEKLELKKEVFHLHEILEEA
                     KDIFEFNQLTHDGKITLQLDAVSDTINGDSVHIGNVIYNLIDNAVKYCKNKPEVIVRT
                     YNEKKGVCLEIEDNGIGIKREHLKMIFDKFYRVSTGNIHDVKGFGLGLYYVGLVVEAH
                     HGTIDVKSTPGKGSTFKIWLPTCVK"
BASE COUNT         1208 a          754 c          754 g         1244 t
ORIGIN      
        1 ggaaaacgat gaaatattgg cagaagatcg catgtcacgt cgtccagaac ttggtcgaat
       61 tttcaaagct tattccgatc gctgttttaa agctggggca atggattttg atgatttatt
      121 gtaccaaatg tatgtgttgc tcagagattt tccggatgta ttgggttact accaacaaaa
      181 gtttcaatac attttggtgg atgagtacca agataccaac tatgcgcagt acaccatcgt
      241 taaaatgttg gctgcagcgt atgaaaatat ttgtgtggtg ggcgatgatg cgcaaagtat
      301 ctactcgttt cgcggtgcca acattcaaaa tatcttcaat tttagagccg attaccccga
      361 tttcaaattg tttaaattgg agcaaaatta ccgcagtaca cgcaatattg tagaagctgc
      421 aaacagtgta attgcgcgca atgtggacca aattaaaaaa gaagtttgga ccgaaaatgg
      481 agaaggagcg ttgctaaacg tcatccgaac attgactgat aatgaagaag gaaagactgt
      541 tgttaaccgt ttgtttgaca taaaaacgca agaaggaatt gaatttagcg attttgcaat
      601 cttgtaccgc accaatcgtc aatcacgttc gtttgaggaa gccttgcgca agatgaatat
      661 tccatacaaa atttatggtg gtatttcgtt ctaccaacgc aaggaaatta aagacatgtt
      721 ggcctatttt agattaaccg ccaaccaaca cgatgaagaa gcgtttaaac gtgttatcaa
      781 ttatcctaag cgtggaattg gaaaaacaac catcgaaaat attaccattg catccaccaa
      841 ttatggagtt tctatttggg atgtggtaac caatttagac caatacccgg tgcaaatgaa
      901 cagcggaaca cgcaccaaaa tcaatgaatt tacaaccatg attttgagtt ttgctgcaca
      961 attggaaaaa tcaaacgcct atgaattggg gcaacacatt gctaaatcga gtgggatttt
     1021 aaaagagttg tacgacgaaa aagaaaaagg tccagaagaa gttgaacgtt accaaaacat
     1081 cgaagaactt atttctggtt tgaaagaatt tacagtttct aaagccgaga cggatgaatt
     1141 tgcatcgctt tcagaattta tgatggatgt tgcacttcta acagatgccg acaacgataa
     1201 tagtgaggat aaaaattatg ttaccttgat gaccatccac tcgagcaagg ggttggagtt
     1261 tccgcacgtg tttttggttg gcttggaaga gaatttattt ccatcgcaaa tggctttgca
     1321 atcgcgaaca gaaactgaag aagaacgccg tttgttttat gttgccgtta cacgcgccat
     1381 gaaatcgtgc acacttactt atgcgtcatc tcgttttcag tggggcaatt taaccagttc
     1441 ggaacccagt cgttttatcg atgaaattga ccctaaatat ttgcatttcg aagcaccaca
     1501 acgcggtcaa ggtcgctcat tgagttcggg cattacctcc aatcgttcaa tgggaggttt
     1561 aaacaaaacg tttgatgctc cacgtccggc tgttgctgca acacctcgca atttgaaacc
     1621 cattgcgaca gttcgaggag gtgcatcgga tagtttagaa tacaaagtgg gctacaatgt
     1681 attgcacgaa cgttttggaa aaggaaaaat tactaaaatt gagggacaag gagctgataa
     1741 aaaggctacc atcttttttc ctcatcatgg agcaaaaacg ttattgttgc gctttgccaa
     1801 tttaacggta gtagaagctt agtttaggct tctacttttt ctaccatttc acatttcatc
     1861 acaccatcac ggtcaatttc agttaattga accatatgaa cggtattggt gagttgcgca
     1921 ttgtagggaa atttcacttt tacataattg tcggtaaatc catgcatgaa gccatcttcg
     1981 ttatcgtttt caaacaacac tgctttagtt ttacccacat tttcttcata aaaagcacgt
     2041 tttttcttat ccgaaagaat gtgtaaacgt ttactgcgtt catgacgcaa agccataggc
     2101 acagaatcgc ccattttgat tgctgttgtg ttagctcttt cagaataggt aaacacgtgc
     2161 aggtacgaaa cagttaaatc tttaatgaaa ttcacagtat cttcaaaatc ctcatccgtt
     2221 tcgcctggaa agccaacaat gacatccaca ccaatgcaac aatcggcatt gtgatttttg
     2281 attgtttcaa tgcgctcagc aaacaattca cgcaagtatt ttctacgcat tttttgcaat
     2341 tgtttgttag agcctgattg caatggaata tggaaatgcg gcacaaagtg gttggagttt
     2401 tctaagcaaa aatgaatgat ttcattgctt aataagttgg gttcgatgga tgaaatacga
     2461 atgcgttcaa cgccttttac tttgtctaat tcttgaatta aaccatagaa attttcttca
     2521 ccaccttgac caaaatcacc aatatttaca ccggcaagta caatttcttt tgcaccttcg
     2581 tcaacagctt tttgtgcttg aacaacagtt tcggcaatgg atgcatttct actttttccg
     2641 cgggcaagag gaatagtaca aaacgtacag aaataatcac aaccgtcttg aatttttaag
     2701 aaagtgcggg ttctatcacc aaatgaaaaa gcaggaacaa attcttttgt ttttttgatg
     2761 ttttcaaaag aaaccaaagc tttgtctttc ttctccaagg tttcgtccac ataatccaga
     2821 atgttgaatt tttcgttggc acccaatact aagtcaacac cccaaatttt tgcaatttct
     2881 tcgggtttta attgagcata acaccccacc accacaataa atgcgttggg gtttactttc
     2941 aaacattttt tgacaatttg cttgcacttg ctatcggctt tttctgtaac cgagcaggtg
     3001 ttgataacat aaatatctgg agtatcctca aactctacct tagcaaaacc agcgttttca
     3061 aacaatctgg caatggtgga tgtttccgca aaatttaact tacatcccaa ggtataaaac
     3121 gcaactttct taaactgtat cataatgaat ggtacaaaat tacatagatt ttagggttta
     3181 ttctataaaa cggaatgaat tttagaagca gtctaaataa tagtccttat ttaacacagg
     3241 ttggcaacca aattttgaat gtacttcctt tacctggcgt actttttaca tcaattgttc
     3301 cgtgatgcgc ttccacaacc aaaccaacat agtaaagtcc caatccaaat cctttcacat
     3361 cgtgaatatt gcctgtagaa acacggtaga atttatcaaa aatcattttc agatgttcgc
     3421 gttttattcc aatgccgtta tcttcaattt ctaagcaaac tccttttttc tcattgtagg
     3481 tgcgaactat aacttcgggt ttgtttttac aatattttac agcattgtcg atgaggttgt
     3541 agataacgtt gccaatatga acagaatcgc cgttgatggt atcgctcaca gcgtctaatt
     3601 gaagggttat ttttccatcg tgggtaagtt ggttgaactc aaaaatatct ttggcttctt
     3661 ccaaaatttc gtgcaaatga aagacttctt ttttcagttc caatttttct ttgtctaatt
     3721 ttgcaatgtt caaaacacgt tccacctgat tttttagtcg attattttct ttaaaaatca
     3781 caccggcata gcgttgaatg tcttcttttt ctgtaattgt tcctttcaac agcatttcac
     3841 tcgataatcc gatggtggaa ataggtgttt ttagctcgtg cgtcatgttg ttgataaaat
     3901 catttttgat gtcagacaag cgtttttgtt tgagaatgac attgatagag taggcaaaaa
//
LOCUS       DAOQVQ010000188         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_25900_length_3941_cov_3.112198, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000188 DAOQVQ010000000
VERSION     DAOQVQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25900_length_3941_cov_3.112198"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..2091)
                     /locus_tag="PKN22_06585"
     CDS             complement(187..2091)
                     /locus_tag="PKN22_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNS42408.1"
                     /translation="MLGILLFESPFVVGFTLFSIFLMLSALVFRKRLENFQFYLLFGP
                     LFTLSGYLMMNNYYPLENKELGQVETYYTGKIVEQMSNDKTWNSALVELQSIRKNKTW
                     EPTSEKVLLVIENSGITYAKNDLLLFNGIFNTIQVRNNPGEFNATMYWLSKGVRYQAF
                     IGENQIRVLQTGTLNWLENVLEQSRNYSNKMLEEWIGEADAPLIKAILLGDKSDIDIQ
                     TKRAFINTGTMHMLAVSGLHIGVIVMLLGLFFKYLFFHRGRHIASIIMLVTLWFYAFL
                     TGFSASVVRAVVMFSILILARFINRDYQPINALSLSAFVILLINPLSIYDIGFQLSYL
                     AMVGIFVYYPLLESVLTIKNKFITTVWQGTAVGLAAQVFTVPISLYYFYQFPNYFFLS
                     NLGVMLFSGIMLGFSIALLLIGKVAIIAAPVGWILALSCVVFVGFIAWIETFYGAVAL
                     GFSPSVWWVLLTYILVLIAIQWVERLKWIWVGLGVLPLLVWIQLERYDNLNKQEWLIF
                     NTNHPTLLINNAGQQMCFYGGGEKGKKNALRLVDDYQKLHPGKVEFLPLEKGVYQVFS
                     NQKNTLQIINKISFLEIKYKNDSYALVVNNSIKELEEFSRKSKLITMQPLEVAATFHD
                     LSKGALRVEL"
     gene            2369..2719
                     /locus_tag="PKN22_06590"
     CDS             2369..2719
                     /locus_tag="PKN22_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS42409.1"
                     /translation="MAIVITDECINCGACEPECPNNAIYEGGAQWRYSDGTSLTGMIT
                     TLDGKDVNADEEFDAIDMDIYYIVPDKCTECEGFHDEPQCAAVCPVDCCVDDPNYRES
                     QDELLAKKTFMHLS"
     gene            2800..>3941
                     /locus_tag="PKN22_06595"
     CDS             2800..>3941
                     /locus_tag="PKN22_06595"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNS42410.1"
                     /translation="MKNKEELIARREAAQLGGGQARIDKQHQGGKLTARERLTLLLDE
                     NSFNEIGQFVEHRATSFGLDKQKFPGDGVVTGYGTIHGRLVYVFSQDFTVLGGSLSET
                     HAAKICRIMDMAMKNGAPVIGLNDSGGARIQEGVVSLAGYADIFYRNTRASGVIPQIS
                     VIMGPCAGGAVYSPALTDFIFMVEDTSYMFVTGPNVVKTVTHEEVSSEDLGGAKAHAE
                     KSGVNHFTASNDVECLKKVRDLLTYMPQNAYELPPQPEKFEFNALKLEAIKSIIPDNA
                     NLPYDIRKVVDAVIDDNSFREVHEDYAKNIVVGFARLEGKTIGIVANQPASLAGVLDI
                     EASRKGARFVRFCDAFNIPLLVFEDVPGFLPGTDQEWNGIITHGAK"
BASE COUNT         1319 a          733 c          689 g         1200 t
ORIGIN      
        1 cggatttgtg gtttttctgg gtttggatgc gccccctgaa aagcttaata ttaaaagtta
       61 tgaatatact tggtcaggag gtggatgaaa actacccggg attaaaagtg attctctact
      121 cagatggaac tacgttaaag aaatattaat aattttaaag agcgttggat tgtccagcgc
      181 ttttttttac aactccactc tcaatgctcc tttgcttaaa tcatgaaaag tagcagctac
      241 ttctaagggt tgcattgtaa ttagtttgct tttgcggcta aattcttcta attctttaat
      301 cgaattattc actaccaacg cataggaatc gtttttgtat tttatttcta aaaaacttat
      361 tttattaata atttgcaatg tgtttttttg atttgaaaaa acttgataaa ctcctttttc
      421 caaaggcaga aactccactt ttccaggatg taatttttgg taatcatcca ccaatctgag
      481 tgcatttttc tttcccttct ctcccccacc ataaaaacac atttgctgcc ctgcattatt
      541 aatcagcaat gttgggtgat tggtattaaa aatcaaccat tcttgtttgt ttaaattatc
      601 atagcgttcc aattgtatcc ataccaaaag aggcaataca cccaatccca cccaaatcca
      661 ttttaaccgc tcaacccatt gaatggcaat taacaccaat atataagtga gcaaaaccca
      721 ccatacagat ggtgaaaacc ccaaagctac tgctccataa aacgtttcaa tccatgcaat
      781 aaaaccaaca aatacaacac acgacaaagc taaaatccaa ccaactggag ctgcaattat
      841 ggcaaccttc ccaataagta gcaaagcaat agaaaaacct aacatgattc ccgaaaaaag
      901 catcacaccc aagttcgata gaaaaaaata attaggaaat tggtaaaaat aatacaatga
      961 aattggcaca gtaaacactt gtgcagcaag accaacggcg gttccttgcc ataccgttgt
     1021 tataaattta ttcttaattg ttaacactga ttctaatagt ggataatata caaaaatgcc
     1081 caccatagct aaatacgata actgaaatcc aatatcgtaa atagataaag gattgatgag
     1141 taaaattaca aaagcagata gcgacaaagc gttgataggt tggtaatccc ggtttataaa
     1201 tcgagcaaga attagtatgc taaacatcac aacagcccta acaaccgatg ctgaaaaccc
     1261 tgtcaaaaaa gcataaaacc ataaagttac tagcatgatg atggatgcaa tgtgtctccc
     1321 cctatgaaaa aacaggtatt taaaaaacaa acccaacaac atcacaatca caccaatatg
     1381 caatccagaa acagccaaca tgtgcatggt tccagtattg ataaacgctc tttttgtttg
     1441 aatatctata tcacttttat cgcccaacaa aatagctttt atcaaaggag cgtcagcctc
     1501 acctatccat tcttcaagca ttttgttaga ataattgcgt gattgttcca atacattttc
     1561 caaccaattt aaagtacctg tttgtagaac acgtatttga ttttcaccaa taaatgcttg
     1621 gtagcgcact ccttttgata gccaatacat ggttgcatta aattctccag gattattgcg
     1681 cacttgaatg gtattaaata ttccattaaa aagtagaaga tcatttttag catacgtaat
     1741 ccctgaattt tcaatcacta aaaggacttt ttcagatgta ggctcccatg ttttgttttt
     1801 acgaatggat tggagttcta ccaacgctga attccacgtt ttatcattgc tcatctgctc
     1861 aacgatttta cctgtataat aggtttcaac ctgtcctaat tccttattct ccaaaggata
     1921 ataattgttc atcattaagt atcccgacaa agtaaacagt ggaccaaata gtagataaaa
     1981 ttgaaaattt tcaagtcttt tcctaaaaac aagtgcgctt agcattaaaa agatagaaaa
     2041 cagtgtaaaa cctactacaa aaggcgactc aaacagcaaa ataccaagca taaagcataa
     2101 aagccaaaaa agcaatgatg attcagttag tattttcata aaagttgtaa taaaaaccaa
     2161 acatactaaa aatttagagt aggtcgcttt ttttgttctc ttgttcttga atgttttttg
     2221 aagttaacaa gtagtagagt tgtttttaaa aatttcttga agaaaaaaaa ccttattcat
     2281 actaattcta attagcaaat ccaataacta aaatcaggta ttttcattaa attttcagta
     2341 tatttgcgca aaatttaaga atcaaaaaat ggcaatagtt attaccgatg aatgcatcaa
     2401 ttgtggtgct tgcgagccgg aatgtccaaa caatgcaatt tatgaaggtg gtgctcaatg
     2461 gcgctattct gacggaactt cattaactgg aatgataaca actttggacg gtaaagatgt
     2521 taacgcagac gaagagtttg acgcaattga tatggatatt tactacatcg tccctgataa
     2581 atgtacggaa tgtgaaggtt ttcatgatga acctcaatgt gcagctgttt gtccggttga
     2641 ttgctgtgta gacgacccta actaccgcga atcgcaagat gaattgttgg cgaagaaaac
     2701 ttttatgcac ctttcttaat cgtttcagct aaatcattat atttgttaaa tccttacata
     2761 ttattacgcc tattgattta aaacattacg cacgctaaaa tgaaaaacaa agaagaacta
     2821 attgctcgta gagaagctgc tcaattgggt ggcggacaag cacgaattga taaacaacac
     2881 caaggcggaa aattaactgc tcgtgaacga ctaacacttt tattggatga aaactcattc
     2941 aatgaaattg gtcagtttgt agaacaccgt gcaacctctt ttggtttgga taaacaaaaa
     3001 tttccaggtg atggtgttgt aacaggttat ggtaccattc acggacgttt ggtttatgtt
     3061 ttttcacaag attttacagt actaggtggc tctttgtcgg aaacacacgc tgcaaaaatt
     3121 tgtagaatca tggatatggc catgaaaaat ggtgctcctg tcattggctt aaacgattcg
     3181 gggggggcac gtatccaaga aggggtggtt tctttggctg gttacgctga tattttttat
     3241 agaaatacac gtgcttcggg ggtaattcct caaatttcgg taatcatggg accatgtgct
     3301 ggaggtgccg tttattctcc tgctcttacc gattttatct tcatggtaga agatacttcg
     3361 tatatgtttg ttaccggacc aaacgttgta aaaacagtaa cacacgaaga agtttcttct
     3421 gaagatttag gtggtgctaa ggcacatgct gaaaaatcag gggttaatca tttcactgct
     3481 tcaaacgatg ttgaatgttt gaaaaaagtg cgtgatttat tgacttacat gccgcaaaac
     3541 gcctacgaac ttcctccaca accagaaaaa tttgaattta atgccttgaa attagaagcc
     3601 attaaatcca tcattcctga taatgccaat ttaccctacg acattcgcaa agtagttgat
     3661 gcggtaattg acgataactc gtttagagaa gtgcacgaag attacgctaa aaatattgtt
     3721 gtaggttttg cacgtttaga aggaaaaact attggtattg ttgccaatca acctgcttct
     3781 ttggctggtg tgttagacat tgaagcgtcg cgtaaaggtg ctcgatttgt acgtttttgt
     3841 gatgcgttca acattccact tttggtattt gaagacgtac caggattctt accaggaacc
     3901 gatcaagaat ggaacggaat tatcacgcac ggagcaaaat t
//
LOCUS       DAOQVQ010000189         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26014_length_3926_cov_3.536037, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000189 DAOQVQ010000000
VERSION     DAOQVQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26014_length_3926_cov_3.536037"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKN22_06600"
     CDS             <1..705
                     /locus_tag="PKN22_06600"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42411.1"
                     /translation="HQTDLVVVLAPGKGSFYPDYFPSQYNGVQPTTTNLEVFKKYFTK
                     NGINYIDANSWFIDLKKTVHQKHKLYSKTGIHWSKYGEYLMADSLIKYLKATTQKPFP
                     SIQLTKLEYSNQLRDTDNDVWKGLNICSTLEDFKMTYPQWKAAHTEFNLAKTLVIGDS
                     YYFGLFKDGFSTNYLNNGEFWYYCKEVYQNVNKTSQPLNLESIKEQALKNDVIIIMCT
                     DATLPRFGFGFIENFK"
     gene            862..1239
                     /gene="rpsL"
                     /locus_tag="PKN22_06605"
     CDS             862..1239
                     /gene="rpsL"
                     /locus_tag="PKN22_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNS42412.1"
                     /translation="MPTIQQLVRKGRTAVAKKSKSAALDSCPQRKGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIGGEGHNLQEHSLVLVRGGRVKDLPGVRYHIVRGAE
                     DTAGVEGRKQRRSKYGAKRPKQK"
     gene            1259..1729
                     /gene="rpsG"
                     /locus_tag="PKN22_06610"
     CDS             1259..1729
                     /gene="rpsG"
                     /locus_tag="PKN22_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNS42413.1"
                     /translation="MRRRKAKKIAVLPDPRFQDVQVTKFVNNLMYDGKKSVAFKIFYD
                     AIELVDKKVEEATGLDTFKKALENVAPPVEVRSRRVGGATFQIPTPVRETRKQSLAMK
                     WLIGYARKRNEKTMAAKLASEIIAASKEEGAAYKKKEDTLRMAEANKAFSHFRF"
     gene            1735..3840
                     /gene="fusA"
                     /locus_tag="PKN22_06615"
     CDS             1735..3840
                     /gene="fusA"
                     /locus_tag="PKN22_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNS42414.1"
                     /translation="MARDLKYTRNIGIAAHIDAGKTTTTERILYYSGVNHKIGETHEG
                     GATTDWMAQEQERGITITSAAVTVPWKYNGNTYQINVIDTPGHVDFTVEVNRSLRVLD
                     GLVFLFSAVDGVEPQSETNWRLANNYNVPRLGFVNKMDRQGADFLNVCRQVKDMLGSN
                     SLPLQLPIGAEDDFKGVVDLINFRGIVWNEADKGMTFTEVEIPADILDEATQMREHLL
                     EQVAEFDDTLMEKYFEDPNSITEEEVLTALRQAVISGKVVPMMCGSSFKNKGVQAMLD
                     MVMALLPSPLDQEAIEGTDPKTGDVISRKPSFDEPFCGLAFKIATDPYVGRLCFVRAY
                     SGKLPAGSYIYNPRTNAKERISRIFQMHANKQNQIDELGAGDIGAVVGFKDIRTGDTL
                     CAEGHGIILESMDFPDPVIGIAIEPKTQADTDKLSIGLNKLAEEDPTFQVHTDEDTGQ
                     TVISGMGELHLDIIIDRLKREFKVEVNQGAPQVSYREAITASVDHREVYKKQSGGRGK
                     FADIVVKISPQDDQEKTGLEFINSIKGGNIPKEFIPSVEKGFKESMKNGVLAGFLVEA
                     MKVELVDGSFHAVDSDSLSFELAAKMAYREALKKCRPILLEPIMKIEVVTPEENMGDI
                     VGDLNRRRAVVRGMDDRNGAKVVKADVPLSEMFGYVTQLRTLSSGRATSSMEFSHYAE
                     APKNVAEEVIAKVNGKVKA"
     gene            3853..>3926
                     /locus_tag="PKN22_06620"
     CDS             3853..>3926
                     /locus_tag="PKN22_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNS42415.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIV"
BASE COUNT         1354 a          615 c          795 g         1162 t
ORIGIN      
        1 caccaaaccg atctggtggt tgttttagct ccaggtaaag gaagttttta tcccgattat
       61 tttccatctc aatacaacgg tgttcaacca acaactacca atttagaagt ttttaaaaaa
      121 tattttacta aaaatggaat caattacatc gatgccaaca gttggtttat agatttaaaa
      181 aagacagtac accaaaaaca caagttatat tctaaaactg gaatacattg gagtaagtat
      241 ggagaatacc taatggctga ttcattaata aaatatttaa aagctacaac tcaaaaacca
      301 tttccatcca ttcaattaac taaactagaa tattcaaacc agttaagaga taccgataac
      361 gatgtatgga aaggattgaa catctgttca acacttgaag attttaaaat gacctatcct
      421 caatggaaag ctgcgcatac ggagtttaat cttgcaaaaa ccttggtaat tggtgatagt
      481 tattattttg gattatttaa agatggtttt tcaacaaatt atttgaataa tggcgagttt
      541 tggtattatt gtaaagaggt ttatcagaat gtaaacaaaa cttcacaacc attaaattta
      601 gaaagcatca aagaacaagc attaaaaaat gatgtcatta ttataatgtg caccgatgca
      661 actttgccac gatttggttt tgggtttata gaaaacttta aatagtttta aaacgattct
      721 ttttttgtaa accgttgaaa atctttcatt ttttttcgca aactattgtt ttgtaaaata
      781 tttgtcatat atttgcaccc cctaaagtgt atttagggga gaattatata ttaattaaat
      841 tttaaaaatt aagagtattt tatgccaact attcaacaat tagttcgcaa gggaagaaca
      901 gcggtagcta agaaatctaa gtctgctgca cttgattcat gtccacagcg taaaggagtt
      961 tgtgtgagag tttacacaac tactccaaag aagccgaact cagcaatgcg taaagttgca
     1021 agggtacgtt tgactaacgg aaaagaggtc aatgcgtata tcggtggaga aggtcacaat
     1081 ttgcaagagc actcattagt gttagtaaga ggaggaaggg taaaagattt acctggagtt
     1141 cgttaccaca tcgtacgtgg tgctgaagac actgctggtg ttgaaggaag aaaacaacgc
     1201 cgttctaaat acggtgctaa gagacctaaa caaaaataac attttaagct gttaagaaat
     1261 gagaagaaga aaagcaaaaa agatagccgt acttcctgat ccacgttttc aggatgttca
     1321 agttacaaag tttgttaaca acttgatgta tgacggaaag aaaagcgttg catttaagat
     1381 tttttatgat gcaatcgaat tagtagataa aaaagtagaa gaagctactg gattggatac
     1441 gtttaaaaaa gcattggaaa atgttgcgcc tcctgttgag gtacgttcta gaagagttgg
     1501 tggagctact ttccaaattc ctactccagt tcgtgaaact agaaagcaat cgttagctat
     1561 gaaatggttg attggttatg ctcgtaaacg taacgagaaa acaatggcag ctaaattagc
     1621 ttcagaaatc atcgctgcat ctaaagaaga aggtgctgct tacaagaaaa aagaagatac
     1681 attaagaatg gctgaagcaa ataaagcatt ctctcacttt agattttaat aacgatggca
     1741 agagatctta aatatacaag aaacattggt attgcagcac acattgatgc tggtaaaaca
     1801 actacaacag agcgtatttt gtattactct ggggtaaacc ataaaattgg tgaaacacac
     1861 gaaggtggtg ctacaactga ctggatggcg caagagcaag aaagaggtat tactattact
     1921 tctgcagcag ttacagttcc atggaaatac aacggaaata cataccaaat taacgtaatt
     1981 gacactccgg gacacgttga ctttacagtt gaggtgaatc gttcattacg tgtattggat
     2041 ggtttagtat tcttattttc tgcagttgat ggtgttgagc ctcaatcaga aactaactgg
     2101 cgtttggcaa acaactataa cgtacctcgt ttaggtttcg ttaataaaat ggaccgtcaa
     2161 ggtgcagatt tcttaaacgt ttgtcgtcaa gttaaagata tgttaggaag taactcttta
     2221 ccattacagt tgccaatcgg tgctgaagat gatttcaaag gagttgttga cttaattaac
     2281 ttccgtggta ttgtttggaa tgaagcagat aaaggtatga ctttcacaga agttgaaatt
     2341 cctgctgata tcttagacga agctactcaa atgcgtgagc acttattgga gcaagttgct
     2401 gagttcgatg atacattaat ggaaaaatat tttgaagatc caaattcaat tactgaagaa
     2461 gaagtattga cggcattacg tcaagctgta atttcaggta aagttgttcc aatgatgtgt
     2521 ggttcatctt tcaaaaataa aggtgtacaa gcaatgttgg atatggtaat ggcattgttg
     2581 ccttctccat tggatcaaga agcaattgaa ggaacagatc ctaaaacagg agatgttatt
     2641 tctcgtaaac cttcttttga cgaacctttc tgtggtttag catttaaaat tgcaacagac
     2701 ccttatgtag gtcgtttgtg tttcgttaga gcttattcag gtaaattgcc agcaggttct
     2761 tatatttaca acccacgtac aaacgcaaaa gagcgtattt ctcgtatctt ccaaatgcat
     2821 gctaacaaac aaaatcaaat tgatgagtta ggtgctggtg atatcggtgc ggttgtagga
     2881 tttaaagata tccgtacagg agatacttta tgtgctgaag gtcacggaat tatcttggaa
     2941 tctatggact tcccagatcc agtaatcggt attgcaatcg agcctaaaac acaagctgat
     3001 acagataaat tatcaattgg attgaataaa ttggctgaag aggatccaac tttccaagtt
     3061 catacagatg aagatacagg acaaactgta atttcaggta tgggtgagtt gcacttagat
     3121 atcatcatcg accgtttgaa acgtgagttt aaagtagaag ttaatcaagg tgctcctcaa
     3181 gttagctacc gtgaggcaat tacagcttct gttgatcacc gtgaagtgta taaaaaacaa
     3241 tcaggtggac gtggtaaatt cgctgatatc gttgttaaaa tctctcctca agacgatcaa
     3301 gaaaaaacag gattggaatt cattaactct atcaaaggtg gtaacattcc aaaagaattt
     3361 atcccttctg tagaaaaagg attcaaagaa tcaatgaaaa atggtgtatt agctggattc
     3421 ttggttgaag caatgaaagt agaattggtg gatggttcat tccacgctgt cgattcagat
     3481 tcattgtctt tcgaattagc ggctaaaatg gcataccgtg aagcattgaa aaaatgtcgt
     3541 ccaatcttgt tagagcctat catgaaaatt gaagtggtta ctccagaaga aaacatgggt
     3601 gatatcgtag gtgacttgaa ccgtcgtcgc gctgttgttc gtggtatgga tgatagaaac
     3661 ggagcaaaag ttgttaaagc tgacgttcca ttgtctgaaa tgtttggtta cgtaactcaa
     3721 ttacgtacat tgtcttctgg tcgtgcaaca tcatcaatgg aattctctca ctatgcagaa
     3781 gctccaaaaa atgttgctga agaagtaatc gcaaaagtta acggaaaagt aaaagcataa
     3841 tacaatagta taatgagcca aaaaattaga attaaattaa aatcatacga tcacaattta
     3901 gttgataaat cagctgagaa aatcgt
//
LOCUS       DAOQVQ010000190         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26090_length_3917_cov_3.947178, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000190 DAOQVQ010000000
VERSION     DAOQVQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26090_length_3917_cov_3.947178"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="PKN22_06625"
     CDS             <1..1678
                     /locus_tag="PKN22_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42416.1"
                     /translation="QNNAIMGTKDTLTYSYYSGGVFDSVDIAFTTHSSTLPGGGFSKN
                     ENVLPYNFYIQNNGGAIYNQFNDWKKMRFSALPHLGFGYVFGTQATQVVKASYAQAFN
                     SQTLLNVDYDMHKGKNFMRAGDFGKHDVQLQFEFQSRFYSLGVKGQFVSRNIAQNDGL
                     LVDSLIDYYGLSFAPIRKENAQSKYRGGRVELSHYFDFLAKDSANAMGLYVDNQLRIF
                     NRKYKEKSDTLTAIYPLIYYNADSTGDQYQLSEIVNAAGLFYSREKLFFKVGVLNNWW
                     NYFNLGNKIAQSEVNLDGTIGLNFKSVAFRNHTNFNLIGANREWYTYSNLDFKWKGLS
                     LNAKANISHLLPEQFQRFYSANNVYYNLLNGNLDKQFKLDVGGHLKYTHQQHQVGVFV
                     NNATVSSNYWFYNNTWRNDTLKTVNAFSVGLTGRTGYKVIFFDFKGSFNKSDWMPSVL
                     VQGRLYVQGRLFKSKKLLAQIGVESSYRTGYKLLEHLPYMDVFNLTNVSTKPMANLHV
                     FGGFEIQRFRFFFRVENLGYFWSDKTTRIALNQTIPSLNIRVGITWDFYN"
     gene            complement(1767..2723)
                     /locus_tag="PKN22_06630"
     CDS             complement(1767..2723)
                     /locus_tag="PKN22_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNS42417.1"
                     /translation="MKILANDGISAVGKKQLEEAGYTVVTDKVNQEDLINAINAENYE
                     ILLVRSATTARKELIDACPNLKLIGRGGVGMDNIDVAYARENGRIVINTPASSSQSVA
                     ELVMGHLFAVSRFLNDSYKNMENGDFNQLKKNYGKGVELRGKTLGIIGFGRIGQSLAS
                     YALGVGMNVIANDQFEYTAEIPVKIGTSQEVIVPIKTSTDLAAFLPQCDYISVHVPKQ
                     ADGSAVLGKNEFALMKNNVRLVNAARGGVIDETALIEALDSGKVAGCALDVFENEPTP
                     NKALLSHPKIACTPHIGAATGEAQDRIGSELAELIIKEFGKK"
     gene            complement(2727..3797)
                     /gene="serC"
                     /locus_tag="PKN22_06635"
     CDS             complement(2727..3797)
                     /gene="serC"
                     /locus_tag="PKN22_06635"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNS42418.1"
                     /translation="MKYNFGAGPCILPQNVFEQAAKGVLDFNGLSILEVSHRHKDFVA
                     VMEEARELVKKALNVPAGYTVLFLQGGATLGFTISALNMTKGGKAGYINTGVWASGAI
                     KEAKLVGVDVEVLASSEDKNFSYIPKNIEVPTNLDYLYYCSNNTIYGTQFHETPKSSV
                     PLVCDMSSDIFSKEINVADYDLIYAGAQKNLGPAGATLYIVKEDALGKSTPKFMPTYL
                     NLAKQHEKESMLNTPPVFSVYVAMLNLKDLLATGGVKAAEERNNAKAAALYNEIDANP
                     LFTTNVAKEDRSKMNVTFVLNEQYAALEEEFNKVWQDAGIVGLKGHRSTGGYRASLYN
                     ALPLSSVQVLVDVMKDFASKKA"
BASE COUNT         1224 a          758 c          688 g         1247 t
ORIGIN      
        1 gcaaaacaat gcaattatgg gaacaaaaga tacgttaacc tacagctatt attcgggtgg
       61 tgtgtttgat tcggtggata ttgcattcac aacccacagt tctaccttgc ccggtggagg
      121 tttttctaaa aacgaaaacg ttttacccta taatttttat attcaaaaca acggtggtgc
      181 aatttacaat caattcaacg attggaaaaa aatgcgcttt tctgctttgc cccacttggg
      241 atttggatat gtgtttggaa cgcaagccac acaagttgta aaagcaagct atgcacaagc
      301 gttcaactca caaacactct tgaacgttga ttacgacatg cacaaaggca agaactttat
      361 gcgagcaggt gattttggaa aacacgatgt gcaactacaa tttgaatttc aatcacggtt
      421 ttattctttg ggtgttaaag gacaatttgt aagcagaaac attgcacaaa acgatggctt
      481 attggtggat tctctgatag attactacgg actttcattt gcacccatta gaaaagaaaa
      541 tgcacaaagt aagtaccgtg gaggacgtgt agaattatcc cattattttg attttttagc
      601 aaaagattcg gcaaatgcaa tgggattgta tgtggataat caattgcgta tattcaaccg
      661 taaatacaaa gaaaaaagcg atacgttaac agctatttac ccgctgatat actacaatgc
      721 agattctaca ggcgaccaat accaactgtc agaaattgtg aatgccgcag gtttgtttta
      781 cagtcgcgaa aaattgtttt tcaaagttgg ggtgctcaac aattggtgga attatttcaa
      841 tttgggcaat aaaattgcgc aatcagaagt gaatttggat ggaacaatag ggttgaattt
      901 caagtcagtt gcttttagaa atcataccaa ttttaatctt attggcgcca atcgagaatg
      961 gtacacctat tccaatttag attttaagtg gaaaggattg tcgttgaacg caaaagcgaa
     1021 tatttcgcac ctacttccag aacaatttca acgcttttat tcggcaaaca acgtgtacta
     1081 caatttgcta aatggcaatt tagataaaca atttaaacta gatgttggtg gacatttgaa
     1141 atacacccac cagcaacacc aagttggcgt ttttgtaaac aatgctaccg tttctagcaa
     1201 ttattggttt tataacaaca catggcgcaa cgatacttta aaaacagtca acgcattttc
     1261 ggttgggcta acaggaagaa caggttacaa agtaattttc ttcgatttta aaggaagttt
     1321 caataaaagc gattggatgc ctagtgtttt ggtgcaagga aggttgtatg tacaaggacg
     1381 tttgtttaaa agcaagaaat tgttggcaca aattggggta gaatcttcct atcgtacagg
     1441 atataaatta ttggagcacc ttccatacat ggatgttttc aatctaacaa atgtttcaac
     1501 caagccaatg gcaaatttac atgtgtttgg aggatttgaa attcaacgtt ttcgtttctt
     1561 ttttagggta gaaaatttgg gatatttctg gagcgacaaa accacccgaa ttgccctcaa
     1621 tcaaaccatt ccttcgttga acattcgagt aggaattacg tgggattttt acaattaatc
     1681 tatcaaccaa gtttaaattt cagttaaaag caaaaaaaaa tgggatttca atgaagaaac
     1741 cccacttaat tattggttga aacaatttat ttttttccaa attctttgat gattaattct
     1801 gctagttcag aaccaatacg gtcttgagct tcgccagttg cagcaccaat gtgaggtgta
     1861 caagcaattt ttggatggct caacaaagct ttgtttggtg tcggttcgtt ttcaaaaaca
     1921 tccaatgcac atccagcaac ttttccgcta tccaacgctt caattaaagc tgtttcatca
     1981 atcacaccgc cacgagcagc gttgaccaaa cgaacgttgt ttttcatcaa agcaaattca
     2041 ttttttccta gtactgcact tccgtcggct tgttttggca cgtgaactga aatgtaatcg
     2101 cattgaggta aaaatgcagc taaatcggtt gacgttttaa ttggaactat cacttcttgt
     2161 gatgtaccga tttttacagg aatttcggcg gtgtattcaa attgatcgtt tgcaatcaca
     2221 ttcataccca caccaagtgc gtaagaagct aagctttgac caatgcgacc aaatccaata
     2281 attcccaatg tttttccacg caattcaact cctttaccgt agtttttctt caattggttg
     2341 aaatcgccat tttccatgtt tttatacgaa tcgtttaaaa aacgagaaac agcaaaaaga
     2401 tgtcccatta ccaattcagc taccgattgt gatgaagaag ccggtgtgtt gataacaatt
     2461 cttccatttt cacgagcata agccacgtca atgttgtcca taccaacgcc tccacgtcca
     2521 atcaatttta aattggggca agcatcaatt aattccttgc gtgctgttgt ggcacttcta
     2581 accaaaagaa tttcataatt ctctgcatta attgcattga ttaaatcttc ttggttaact
     2641 ttgtcagtaa caactgtata tccagcttct tccaactgtt ttttccctac agcagaaatg
     2701 ccgtcgtttg ctaatatttt cattttttat gcttttttac ttgcaaaatc cttcatcaca
     2761 tccaccaata cttgcacact tgaaagtggt aacgcattgt acaaagaagc acggtaacca
     2821 cctgttgaac ggtgaccttt taatccaaca atgcctgcat cttgccatac tttgttgaat
     2881 tcttcttcta acgctgcata ttgttcattt aaaacaaatg taacgttcat tttagaacga
     2941 tcttcttttg caacgttggt tgtaaacaaa ggattggcat caatttcatt gtacaaagca
     3001 gctgcttttg cattgttgcg ttcttctgcc gctttaacgc caccagttgc caacaaatct
     3061 tttaaattca acatggcaac ataaacagag aatacaggag gagtattcaa catggattct
     3121 ttttcgtgtt gttttgccaa attaaggtat gtaggcataa acttaggagt agatttaccc
     3181 aaagcatctt ctttaacaat gtaaagtgtt gctccagctg gacctaaatt cttttgagca
     3241 ccagcgtaaa tcaaatcgta atctgcaacg ttgatttctt ttgagaaaat atccgaagac
     3301 atatcgcata ccaacggaac agatgatttt ggagtttcgt ggaattgtgt tccataaatt
     3361 gtattgttag aacaatagta caaataatcc aaattagttg gaacttcaat gttttttgga
     3421 atgtaagaga agtttttatc ctcagacgaa gccaaaacct ccacatcaac acctactaat
     3481 tttgcttctt tgattgcgcc acttgcccat acacctgtgt tgatataacc cgcttttcca
     3541 cctttagtca tgttcaacgc agaaattgta aatccaagcg ttgcaccacc ttgcaaaaat
     3601 aaaaccgtat atcctgctgg aacattcagg gcctttttaa ccaattctct tgcctcttcc
     3661 attacggcaa caaaatcttt gtgtctgtga gatacttcta aaatggatag accgttaaag
     3721 tctaaaacac cttttgcagc ttgttcgaaa acattctgag gtaagatgca tggacctgct
     3781 ccaaaattat atttcatttt tcttaaaatt attatttact aatcaataca atagcaatca
     3841 acaagtgttt tttaacttat tgaaaagcag tgactaacaa aacagaagct attaatcttc
     3901 ttttttaact ctctcat
//
LOCUS       DAOQVQ010000191         3881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26418_length_3881_cov_3.723471, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000191 DAOQVQ010000000
VERSION     DAOQVQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26418_length_3881_cov_3.723471"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKN22_06640"
     CDS             <1..628
                     /locus_tag="PKN22_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42419.1"
                     /translation="LTDPVEILPFPGQKLRDKQNKIPAQKPSVAPTNPSISQRMYEKP
                     EPKVLQKAGEKLVTTNLSIKKIVAEQTNNQAQQAAIQNEPAEEFTIDQVNMFWRQFAN
                     RMKDEGKETFYNALVKRNPKQITNVLYQLEVDNNVQIEVIKTDFDRFMTFMRNSLKNF
                     AFFVEVKVTDNQSEDAKHLNGKDKFAILSRKFPYINTLKDTFNLDIDY"
     gene            complement(629..2626)
                     /locus_tag="PKN22_06645"
     CDS             complement(629..2626)
                     /locus_tag="PKN22_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42420.1"
                     /translation="MKITMLFNVLGVKSTLLLLFLVICYSANSQVLVYNFDDSPTANP
                     YTTAPSPVDAQLSNFSWTNTVGAFSNIGGNTGSSLSISNSSGTPSYTLTFDIAAGCEL
                     NLSGFSFWARRSNTGAQNWDLKVNGTSVGTGTVPTSGSVISGTCSMLLTGTVTITLEL
                     SGGSSVTGSFRLDDFTLNGSVSCLSSNTITASNLSSTSYSVDCTTGANSSIDFTSDGV
                     FNAGNTFTAQLSNATGSFASPTSIGNIALSGTDPSGTINFTIPAQTASGSNYLIRIVS
                     SNPVTTSNSTTTITITLTGAPCVLTQPHITSLIYNGCNSIPSCSGVTEGITEIVFANT
                     GGYSLLAISNNIDINYPGSPAYDIMGSVVNNSTTTAAINTQAGCGTLYLDGYNQTIPP
                     NSIMLFLPSNIPVCAFDWSSMCGQGPVYVIYGQSGSSGDTWHTGGNFGNSSGVKNFSV
                     SFTTTDGTTHTNPYSYNGGSGDGAYATYGSNPVGGSAATQGVFTNCAFDPIIVLGSEV
                     VSFTGEKNNGVAYLNWRVETERNNDYFSIHRSTDGVNWTLIDNVDGRGSATTLKDYQL
                     IDFEPEMGVNYYRLTSTEFNGQRHTHGIIPLEFNQTMVHYDAANALLKFKDNVVATIY
                     AVNGDLVAKVNNQNSINFTKKGMFIIHYEETGTMERLIIAE"
     gene            complement(2724..3437)
                     /locus_tag="PKN22_06650"
     CDS             complement(2724..3437)
                     /locus_tag="PKN22_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42421.1"
                     /translation="MQIGTDFPKIEFQLFGLDLLEPNGFIGNFLIFLLSMYFYFKVKK
                     IEQQGSNDFLKNWRLFYLFFGFSFFLGGFGHLLYNYFQVAGKIPSWFFSLFAPYYIEN
                     AMISIYPNEKRKPLYRKISLLKLVILIAAEAILLINMDISHKPEIGLIIPSLASVLGL
                     VLCLGVLGIYYKTKIHDSFINLWYAMLLLFFSLLPQIFKFNIHPYWDRNDLSHFLLLF
                     TLVLYYQAVKKHHEFELTH"
     gene            complement(3537..3607)
                     /locus_tag="PKN22_06655"
     tRNA            complement(3537..3607)
                     /locus_tag="PKN22_06655"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3573..3575),aa:Cys,seq:gca)
BASE COUNT         1381 a          710 c          637 g         1153 t
ORIGIN      
        1 cttaactgat cccgtagaaa ttctaccttt tccaggccag aaattacgcg ataaacaaaa
       61 taaaattcca gcccaaaagc cttcggtagc gccaaccaat ccttcgataa gtcagcgcat
      121 gtatgaaaaa cccgaaccaa aagtattgca aaaggcgggt gaaaagctgg taacaaccaa
      181 tttatccatc aaaaaaattg ttgccgagca aacaaataat caagcacaac aggctgctat
      241 tcaaaatgaa ccagcagaag aatttacaat agaccaagta aatatgtttt ggcgtcagtt
      301 tgccaatcgc atgaaagatg aaggaaaaga aacgttttac aatgccttag tgaaacgcaa
      361 tccaaaacaa attaccaatg ttttatacca attagaggtt gacaacaacg tgcaaattga
      421 agttataaaa acagattttg atcgttttat gacgtttatg cgcaactccc taaaaaactt
      481 tgctttcttt gttgaagtta aagttacaga caaccaatcc gaagatgcaa aacacttaaa
      541 cggcaaagat aaatttgcaa ttctttctcg taaattccct tatatcaaca cattaaaaga
      601 cacattcaat ttagatattg attattgatt attcagcaat gatcaatcgt tccattgtac
      661 ctgtttcttc gtagtgaatg ataaacattc cttttttagt aaagttgatg ctattttgat
      721 tatttacttt tgcaaccaaa tcaccgttta ctgcataaat agttgcaaca acattatctt
      781 tgaatttcaa caatgcattt gccgcatcgt aatgcaccat tgtttgatta aactcaagag
      841 gtataattcc atgcgtatgt ctttgtccat taaattctgt agatgttaaa cggtaatagt
      901 tgacacccat ttcaggctca aaatcaatca attgataatc ttttagggta gttgcagaac
      961 ctctaccatc tacgttgtca attaaggtcc aattaacacc gtcagtggaa cgatgaatag
     1021 aaaaataatc gttgtttcgt tctgtttcaa cacgccaatt taagtaagca acgccgttgt
     1081 ttttttcacc cgtaaaacta acaacttcac ttcccaacac aattattgga tcaaacgcac
     1141 aattagtaaa cacaccttgc gttgcagcac ttccacctac tggattggaa ccatacgtgg
     1201 cataagcacc atctccactt cctccattat aggaatatgg attggtatgt gttgttccat
     1261 ctgttgtggt gaaagaaaca ctaaagtttt taacccctga tgaatttcca aagttaccac
     1321 ctgtatgcca agtatctcca cttgagccag attgtccata aataacatac acaggtcctt
     1381 gaccacacat cgagctccaa tcaaaagcac acaccggaat attacttggc aaaaacaaca
     1441 ttattgaatt gggtggaatg gtttggttat agccatctaa ataaagtgtc ccacaaccag
     1501 cttgtgtatt tattgcagct gtagttgtag aattatttac aacacttccc ataatatcat
     1561 aagctggact accaggataa ttaatatcaa tattgtttga aatcgcaagc aaactatacc
     1621 ctcctgtatt tgcaaaaacg atttctgtaa tcccttcagt gacaccagaa caagatggaa
     1681 tactattgca tccattataa attaaacttg tgatatgtgg ttgtgttagc acacaaggcg
     1741 ctcctgttaa tgtgattgtt attgtggtag ttgaattact tgtagttact gggtttgaac
     1801 taacaatacg aatcaaatag ttgcttccac ttgccgtttg agctggaatg gtaaaattaa
     1861 tcgtaccact aggatctgtt ccactcaagg cgatgtttcc aatggatgtt gggctagcaa
     1921 aactccctgt tgcatttgac aattgagcgg tgaaggtatt accagcatta aacacaccat
     1981 cagatgtaaa atctatagag ctatttgctc cagttgtaca atctacagaa taagaggtag
     2041 aagataaatt ggaggctgta attgtgttag acgacaagca cgatacactt ccgttgagtg
     2101 taaaatcatc caaacgaaat gaacctgtta cgctactccc tcctgataat tcaagagtaa
     2161 tggtaactgt gccagtaagt agcatagaac aagtacctga aattacactt ccactcgttg
     2221 gtacagtacc agtaccaacc gaagtcccat tgacttttaa gtcccaattt tgcgctcctg
     2281 tgttacttct tctcgcccaa aaactaaaac cacttaaatt caattcacaa ccagcggcaa
     2341 tatcaaatgt gagggtataa ctaggtgttc ccgaagaatt tgaaatagac aatgaactcc
     2401 cggtatttcc accaatatta ctaaatgcac caactgtatt ggtccaagaa aaattggata
     2461 gttgtgcatc tactggcgaa ggagctgttg tatagggatt tgctgtaggg ctgtcatcaa
     2521 aattatacac caaaacttgt gaatttgcac tgtaacaaat aactagaaat aatagtaaca
     2581 acgtactttt cacacctaaa acattaaaaa gcatagtaat tttcatgttt ctaatttatt
     2641 aaaaaaaatt gatttgtatc aaaattattt aataaaataa caaataattg aaagtagtaa
     2701 aaaggtaact aaaaagtaat tctttaatgc gttagctcaa actcatgatg ctttttaacc
     2761 gcttgataat aaagaactaa agtaaacaga agtaaaaaat ggctcaaatc gtttctgtcc
     2821 cagtatggat gaatgttgaa tttaaaaatt tgtggaagga gggagaagaa aagcaacaac
     2881 atagcatacc acaaatttat aaaagaatcg tggatttttg ttttgtaata aatacccaat
     2941 acacccaggc aaagcaccaa accaagcaca gatgccaaag aaggaatgat tagtccaatt
     3001 tctggtttat gagaaatatc catattaatc aataaaattg cttctgcagc gataagtatc
     3061 actagtttta gtagtgaaat tttacgatac aacggttttc ttttttcgtt tggataaata
     3121 gaaatcattg cgttttctat ataataaggc gcaaacagtg aaaagaacca cgatggaatt
     3181 ttgcctgcaa cttgaaaata gttgtacaat aaatgtccaa atccacctaa aaaaaagcta
     3241 aaaccaaaaa acaagtagaa aagtcgccaa ttttttaaaa aatcattgct tccttgttgt
     3301 tctatttttt ttactttaaa ataaaaatac atggaaagta gaaaaattaa gaaattacca
     3361 ataaaaccgt tgggttctaa taaatccagt ccaaacaatt gaaattctat tttaggaaag
     3421 tcggtgccaa tttgcataat gtgtgtttgc gttttatgtg tattgctgca aaaaacggtt
     3481 aaattaggaa tttagtataa aaaaaaggct cttttaacag agcctttaat ttgtagaggt
     3541 cccgagcgga ttcgaaccgc tgtagacggt tttgcagacc ggtgcctagc ctctcggcca
     3601 caggaccctt tttattgggg acacaaactt acaaattatt tctgaaataa ctaataaaat
     3661 tcacaaaaag acactaaaaa agtaatttca aaaaaaatga agtagttaac ttattaattt
     3721 taaatgtgtt ttaaggttta attaaaataa aattaaaaaa gttcgtcaaa acaattgtta
     3781 aattaaaata aatacataac tttgcacccc acaaacagta aaagaactgt ttttagattc
     3841 cgtagctcag ctggtagagc aatacacttt taatgtatgg g
//
LOCUS       DAOQVQ010000192         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26444_length_3878_cov_3.792571, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000192 DAOQVQ010000000
VERSION     DAOQVQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26444_length_3878_cov_3.792571"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1010
                     /locus_tag="PKN22_06660"
     CDS             99..1010
                     /locus_tag="PKN22_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNS42422.1"
                     /translation="MKYIFLFILCLCAFKANAQQDEQMSIYMYNPLYFNPAYAGSKDA
                     ISMTAVGRFQWIKFSGAPRSQWFSIHAPLLFKSLGIGAHMVNDKIGKRNRTSVFADIS
                     GSIQVSKKNQSRIALGLSVGADVMAFDFTSSVVNDVNDPFFGQRINTTKPNIGAGIYY
                     YGNKHYIGISSPRLLEASIKTDLDLVKQINARHFFLAAGYVFDLNSVLKLKPSTLVKF
                     TPHAPITFDVNVSLLSYERLWTGLMYRFNESIGANIGIIIKDFTFGYVYDFPINGLMK
                     YQSGSHEIFLQLDIHTKRIPYTSPRYF"
     gene            1012..3162
                     /locus_tag="PKN22_06665"
     CDS             1012..3162
                     /locus_tag="PKN22_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS42423.1"
                     /translation="MKLIITSIFIVVSVACFGQLKQKVADNLFKRMEYYECVEMYNEL
                     ANKTIKGKGVENLENVRKAAISNYELFRMKEAISYFEFLDKKANLSEKDREFYIQALR
                     YSSNYDKAEKQVGIAYSLYPNNTYFSQLKNELKAFNQLFADSRNISVKKTNISSIFGD
                     FSPVYYKNGLIYATKSKNTEVLNGRYKWDNSYYVSLMESNFASDSTIGNGKLLRHQFL
                     DKAHDGPVAFTKDQQKMVITKNKFGKRKGKDVVVLALYFSELVDGKWTKLVPFEYNND
                     AYNVGHGCFSEDGSTLYFASDMEGGYGQTDIYKTQWVDGKWSKPENLGSHINTNLQEM
                     FPFVVEDRLFFASSGHFGLGGLDLFEASLTKNTTPVNLGYPINTSTDDFGMICELQTD
                     GIKGFFSSNRKDNIDGIYAFTKKDVQLELVVNVYEKYTTNEPVAQQPISLTNQTTGEK
                     QEFFTNNEGNVRLMIRKNENYVVSTAKSDFKLLKEENVSSEGIQKDSVLTADLFLLPT
                     KITIALRVIAKDTRKPLNEAATTVSHFSTNWDTTLVTNEHGLVTLVVDRNKNFWAHAA
                     KKGYIDADRGFNTSNEDGKIIELELELTPIKKGEKFKLENIFYDLNKATLRPESMASL
                     DKLADFILKNELKIELSAHTDSRGSDAYNLKLSQARAQSCVNYLLTKGVKPAQIRAKG
                     YGETQLVNRCKNGVTCTEDEHQENRRTEVKILDY"
     gene            3356..>3878
                     /locus_tag="PKN22_06670"
     CDS             3356..>3878
                     /locus_tag="PKN22_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNS42424.1"
                     /translation="MQRVRDQQRDGGGQQFRGRSAQEIRDYFQHRAGRTDVPTILMAL
                     SAAVMKADGKVLKIELDYVKAFFKQQFGDQFNVLHLQSLKNYLESDIPLNDICSDVRL
                     HLRPESRIQLIHYLFGIANVDGGVSQSELHVIADIAYKMGIPQVDFDSVKNMFFRDVN
                     SDYKILGITEPATD"
BASE COUNT         1308 a          641 c          750 g         1179 t
ORIGIN      
        1 caactggaac ctatttttat gtgttgattt tgaacgatgc tgataatacc cctccgttca
       61 aaggatacat tgaagttgaa tactaaaact actttttgat gaaatatatt tttctattta
      121 tactatgctt atgtgcgttt aaggcaaatg cacagcaaga cgagcaaatg tccatctaca
      181 tgtataatcc gttgtatttt aatccagcgt atgcaggtag taaagatgcc attagcatga
      241 cagctgttgg acgtttccaa tggataaaat ttagcggtgc tccacgctct caatggttta
      301 gtattcatgc tccactttta ttcaaatcgt tgggaattgg agcacacatg gtgaacgata
      361 aaattggtaa acgcaaccgc acatctgtgt ttgccgatat tagcggaagc atccaagtga
      421 gtaagaaaaa ccaaagtaga atagcgcttg gactttcggt tggtgcagat gtaatggcat
      481 ttgattttac ttcatcggtt gtgaacgatg tcaacgaccc attttttggt cagcgaataa
      541 acaccacaaa acccaacatt ggtgctggaa tttattatta tgggaataag cattacatcg
      601 gtatttcctc tccacgttta cttgaagcat ccatcaaaac tgatttggat ttagtaaaac
      661 aaatcaatgc tcgtcacttc tttttggcag ctggttatgt gtttgactta aattctgtat
      721 tgaaattaaa accttcaaca ttggtaaaat ttacaccgca tgcaccaata acctttgatg
      781 taaatgttag tttattatcc tatgaacgtt tgtggacagg tttaatgtat cgtttcaacg
      841 agtcaatagg agccaacatt ggaattatta tcaaagattt tacttttggt tatgtctatg
      901 attttccaat caacggattg atgaaatacc aaagtggatc gcatgaaatt tttctgcaat
      961 tagatatcca taccaaacgc ataccttata cttcaccaag atatttttaa gatgaaacta
     1021 ataattacaa gcatatttat tgttgtgtcg gttgcttgct ttggtcaatt gaagcaaaaa
     1081 gttgccgaca atcttttcaa acgcatggaa tattatgaat gtgttgaaat gtacaacgaa
     1141 ttagcaaaca aaaccataaa aggcaagggt gtagaaaact tagaaaatgt aagaaaagca
     1201 gccatttcta actatgaact ctttcgcatg aaagaagcca tttcgtattt tgagtttttg
     1261 gataaaaaag ccaatctaag tgaaaaagat cgcgaatttt acattcaagc gcttcgttat
     1321 tccagcaatt atgataaagc tgagaaacaa gtgggaattg catacagttt gtatcccaac
     1381 aatacctatt tcagtcagct taaaaatgaa ttaaaggcgt ttaaccaatt atttgcagat
     1441 tccagaaaca tttcggtcaa aaaaaccaat atttcatcca tttttggtga tttttcgcct
     1501 gtttattaca aaaatgggct tatttatgcc acaaaatcaa aaaatacaga ggtactaaac
     1561 gggcgctaca aatgggataa ttcctactat gttagcttga tggaaagtaa ttttgcatcg
     1621 gattcaacta ttggaaatgg taaattgttg cgacatcaat ttttggataa agcgcacgat
     1681 ggcccagttg catttaccaa agaccaacaa aaaatggtta ttaccaaaaa taaattcgga
     1741 aaacgaaaag gaaaagacgt ggtggtttta gcactatatt tttctgaatt ggtggacgga
     1801 aaatggacga aattagtacc gtttgagtac aacaacgatg cctataacgt tggtcacggt
     1861 tgtttttcgg aagatggtag taccttgtat tttgcttcgg atatggaagg aggatatggt
     1921 caaacagata tctacaaaac tcaatgggta gatggaaaat ggtcgaagcc agaaaatttg
     1981 ggatcgcaca tcaataccaa cttgcaagaa atgtttcctt ttgtggtaga agatcgtctg
     2041 ttttttgctt cgtcagggca ttttggattg ggaggtttgg acttgtttga agcgagctta
     2101 acaaaaaaca cgacaccagt taacttgggt tatccaatca atacctctac cgatgatttt
     2161 gggatgattt gtgaactaca aaccgacgga ataaaaggat ttttctcctc caaccgaaaa
     2221 gataacattg atggaattta tgcttttacc aaaaaggatg ttcaattgga attagtggta
     2281 aacgtgtatg aaaaatatac taccaatgaa cctgtggcac aacaaccaat ttcgctcaca
     2341 aaccaaacaa caggtgagaa acaagagttt tttaccaata acgaaggaaa tgtacgtttg
     2401 atgattcgta aaaatgaaaa ctatgtagtg agcaccgcaa aatctgattt taaactactc
     2461 aaagaagaaa atgtttcatc agaaggtatt caaaaagatt cggtgctaac agctgatttg
     2521 tttttattgc caactaaaat cacaattgct ttgcgcgtta ttgcaaaaga taccagaaaa
     2581 ccattaaacg aagctgcaac aaccgtttct catttttcaa ccaattggga tacgacttta
     2641 gtaacaaacg aacatggttt ggtgactttg gtagtagata gaaataaaaa tttctgggcg
     2701 cacgcagcaa aaaaaggcta cattgatgcc gatagaggct ttaacacttc taatgaagac
     2761 ggaaaaataa ttgaattgga attggagtta acgcccatta aaaaaggaga gaaatttaag
     2821 ttggaaaata ttttctatga cttaaataaa gctactttgc gaccagaaag tatggcttca
     2881 ttggataaat tggccgattt tattcttaaa aatgaattaa aaattgaatt aagcgctcac
     2941 accgattcac gaggtagtga tgcttacaac ttaaaactat cgcaagcacg tgctcaaagt
     3001 tgcgttaatt atttgctcac caaaggtgta aaaccagcac aaattcgagc aaaaggttat
     3061 ggtgaaacgc aattggtaaa ccgttgtaag aatggtgtaa catgtacaga agacgaacac
     3121 caagaaaaca gaagaacaga agtgaagatt ttggattatt aagagtccaa agaattttgt
     3181 tacatttttt taagaaaaag tacgcaagat atcccatcaa aaaagattat tttcgcactt
     3241 ttattttaaa atacactttg tgtttggttt aattttagga ttcgtttttt attttgctac
     3301 taaaagtttt tttggtgcgt tgtttggatt ttttataggt tcatacattg acaatatgca
     3361 acgtgtccgt gaccaacaaa gagatggtgg cggacaacaa tttagaggac gttcggcaca
     3421 agaaatcaga gattattttc aacatcgcgc aggaagaacg gatgttccaa ccattttaat
     3481 ggcgctttct gccgctgtga tgaaagccga tggcaaggta ctaaaaattg aattggatta
     3541 tgtcaaagca tttttcaaac agcaatttgg cgatcaattc aatgtgttgc atttgcaaag
     3601 ccttaaaaat tatttagaat ccgatattcc actcaacgat atttgctccg atgtacgctt
     3661 gcatttgcgt ccagaatccc gcatccaatt gattcactat ttatttggta ttgccaacgt
     3721 tgatggtgga gtatcgcaaa gcgaattgca tgtaatagcc gatattgcct ataaaatggg
     3781 gattccacaa gtggattttg attcggtgaa aaacatgttc ttccgcgatg tgaattcaga
     3841 ttacaaaatt ttaggaatta ccgagccagc aactgatg
//
LOCUS       DAOQVQ010000193         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26526_length_3870_cov_5.452163, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000193 DAOQVQ010000000
VERSION     DAOQVQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26526_length_3870_cov_5.452163"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PKN22_06675"
     CDS             <1..1533
                     /locus_tag="PKN22_06675"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNS42425.1"
                     /translation="DEFRDYILGFIFYKYLSEKMEMFANNILQQDKITFRNITPAMPQ
                     ADEYLEAIKDEALEKLGYFLRPEELFTEVAKRGMGNNEDMDQLDESSNDTINRVFILE
                     DLQKILTNIQLSTMGTDSEEDFDNLFEDMDLNSTKLGKTPDARNTIIAKVLSHLDKID
                     FKLENTELDVLGDAYEYLIGQFASGAGKKAGEFYTPQEVSKILAKIVTTGKQKLKSVY
                     DPTCGSGSLLLRVAREVKDVAMFYGQEMNRTTYNLARMNMILHDVHYRKFDIKQEDTL
                     EHPQHLDDLPFEAIVANPPFSAKWSANPIFTSDDRFSQYGKLAPSSKADFAFVQHMIY
                     HLAENGTMAIVLPHGALFRGGAEQHIRKFLIENKNYLDAVIGLPANIFYGTSIPTCIM
                     VFKKCRENPDDVLFIDASNDFEKVKTQNILREQHINKIIDTYRNRAEIDKYSKKAGLQ
                     EIASNDYNLNIPRYVDTFEEEEQIDIDAIALELKTLDETIEKTDASIAVFCKELGIET
                     PF"
     gene            1549..2148
                     /locus_tag="PKN22_06680"
     CDS             1549..2148
                     /locus_tag="PKN22_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNS42426.1"
                     /translation="MDKFKNKYRIPSARLQNWDYGSNGAYFITICTQNREHFFGEIVD
                     GKMQLNELGQLAEKYWLEIPDHFPFIELGNFVIMPNHTHGILILDSDSDTGSDTGSNC
                     RDAINRVSTIGTTADNTTTGGVTGNKNPMLHQNISRIIRWYKGRCSFEMRKIHADFAW
                     QSRFHDHIIRDTPSFKRIQTYIDNNPMNWNKDKFYENNN"
     gene            2132..3466
                     /locus_tag="PKN22_06685"
     CDS             2132..3466
                     /locus_tag="PKN22_06685"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNS42427.1"
                     /translation="MKTTINKNQNIPPIRFPEFTGEWEKKKLGEVATNKSGKYNPEKE
                     SSSVKCIELEHLSSETGQLIGYIDGKKSGSIKNTFEKGDVLFGKLRPYLKKYLLAPFD
                     GVCSSEIWVLKGMKISNDFLYRIVQTDRFIDLANQSSGSKMPRADWNVIEKGVFSFPS
                     LPEQTKIASFLTAVDEKIQALKQKKNGLEQYKKGVMQRIFCLHHDSLDVHDEHDFEEE
                     TQENTQEKNQGNQENHKKSRFRQLRFRQDDGSDFPKWEVKKLGEVCFEHQLKNKNNET
                     NEVFSVAKHKGVINQIEHLGRSFSAKEIFHYKLIYPGDLVYTKSPTSDFPFGIIKQNR
                     TGRIGVVSPLYCVFKPQTYALGYILHEYFNSAINTLNYLSPLVQKGAKNTMNINNDTF
                     MNGARLLLPTDEKEQEKIANFLSALDEKINRTQTQIEQTERWKKGLLQQMFV"
     gene            3654..>3870
                     /locus_tag="PKN22_06690"
     CDS             3654..>3870
                     /locus_tag="PKN22_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42428.1"
                     /translation="MAKQSEQILEEQLVVNLQKLGYQYLSINDEKGLLTNLKNQLEKH
                     NQISLSDAEFDKVLNILNKGSVFEKAKT"
BASE COUNT         1388 a          653 c          733 g         1096 t
ORIGIN      
        1 gatgaatttc gcgactatat cttgggcttt attttctaca aatacctgag cgagaaaatg
       61 gagatgtttg ccaacaatat tttgcaacaa gacaaaatca cctttaggaa tattacacct
      121 gcaatgcctc aggccgatga gtatttggaa gctatcaaag atgaagcatt ggaaaaatta
      181 ggttattttc ttcgcccaga agaacttttt accgaagtgg caaaaagagg aatgggcaac
      241 aatgaagaca tggaccaatt ggatgaaagc agtaatgaca cgattaatcg cgtttttata
      301 ttggaagacc ttcaaaaaat actaaccaac atccaattaa gtacaatggg tacagacagc
      361 gaagaggatt ttgacaacct ctttgaagat atggatttga acagcactaa actcggtaaa
      421 acgcccgatg caagaaatac aattattgcc aaagtacttt cgcatcttga taagattgat
      481 tttaaattgg agaacaccga attggatgtt ttgggcgatg cctacgaata tctgattgga
      541 cagtttgcca gtggcgcagg aaaaaaagct ggtgaatttt acacccctca ggaagtttct
      601 aaaatattgg caaaaattgt taccacagga aaacaaaaac taaaatcggt gtatgatcct
      661 acgtgtggct ctggttcttt gttgttgcgt gtagcccgag aagtgaaaga cgtggctatg
      721 ttttacggtc aagaaatgaa ccgtactacg tacaacttgg ctcgcatgaa tatgattttg
      781 cacgatgtcc attatcgaaa atttgacatc aaacaagaag atacgttgga gcatccgcag
      841 catttagacg atttgccttt tgaagccatt gttgccaatc cgcctttttc tgccaaatgg
      901 agtgccaacc caatttttac gagtgatgac cgttttagtc aatacgggaa attagcacca
      961 tcaagcaagg ccgattttgc ttttgtgcaa cacatgattt atcatttggc tgaaaatgga
     1021 acgatggcca ttgttttgcc acacggtgca ttgtttagag gtggcgcaga acaacacatt
     1081 cgtaaatttc taattgaaaa caaaaactat ttagatgctg tgattggctt gcctgccaat
     1141 attttttatg gcaccagtat tcctacttgc attatggttt tcaaaaaatg tagggaaaat
     1201 ccagatgatg ttttgtttat cgatgccagc aatgattttg aaaaagtaaa aacacaaaat
     1261 attttacgcg agcagcacat caacaaaatc attgacacct atcgcaatcg tgcagaaatt
     1321 gataaataca gcaaaaaagc cggtttgcaa gaaattgcaa gcaatgatta taatcttaac
     1381 attccacgct atgtggatac ctttgaggaa gaagaacaaa ttgacattga tgccattgcc
     1441 cttgaattga aaaccttgga cgaaactatt gaaaaaacag atgcatccat tgccgtattt
     1501 tgtaaagaat tggggataga aacaccattt taataggtat tttgaaaaat ggataaattc
     1561 aaaaataaat accgcatacc gtcagcccga ttgcaaaatt gggattatgg cagcaatggc
     1621 gcctatttta ttaccatttg cacccaaaac agggaacatt tttttggtga aattgtggat
     1681 ggaaaaatgc aattgaacga attgggacaa ttggcagaaa aatattggtt ggaaataccc
     1741 gatcattttc catttattga attgggaaat tttgtgataa tgcccaacca tacgcatggg
     1801 atattgattt tggattccga ttccgatacc ggttccgata ccggttccaa ttgtagagac
     1861 gcgattaatc gcgtctctac aattggaacc accgccgata ataccaccac cggcggcgtt
     1921 acaggaaata aaaatccgat gttgcaccaa aatatttccc gaattattcg atggtacaaa
     1981 ggacgttgtt cgtttgaaat gcgtaaaatc cacgccgatt ttgcctggca atcccgtttt
     2041 cacgaccata ttattcgtga tacaccatca tttaaacgca tacaaaccta tattgataac
     2101 aatccaatga attggaataa ggataaattt tatgaaaaca acaattaata aaaaccaaaa
     2161 cataccacca atccgcttcc ctgaatttac aggcgaatgg gaaaagaaga aattaggtga
     2221 agtggcaaca aataaatcag gtaaatacaa ccccgaaaaa gagtctagtt ctgtaaaatg
     2281 tatagaatta gaacatcttt cttctgaaac tggacaatta attggttata tagatggaaa
     2341 aaaatctgga agtataaaaa atacatttga gaaaggtgat gtgctttttg ggaagttacg
     2401 accttatttg aaaaagtatt tattggcacc atttgatggt gtttgctctt cagagatttg
     2461 ggttttaaaa ggcatgaaga tatcaaatga ttttttgtat agaattgttc aaacagatag
     2521 gtttattgat ttagctaatc aatcaagtgg gtcaaaaatg cctagagctg attggaatgt
     2581 aattgaaaaa ggtgtctttt ccttcccctc tctccccgag caaaccaaaa ttgcctcctt
     2641 tctcacagcc gtagatgaaa aaatacaagc cctcaaacaa aagaaaaacg ggctggagca
     2701 atacaaaaaa ggggtgatgc agaggatatt ttgtctgcac catgattcac ttgatgtaca
     2761 tgatgaacat gattttgaag aagaaactca agaaaatacc caagaaaaaa atcaaggcaa
     2821 tcaagaaaat cataaaaaat caaggttcag acagttgcga ttcagacaag atgacggcag
     2881 cgattttcca aaatgggaag tgaaaaagtt gggggaggtg tgttttgaac atcaattaaa
     2941 gaataaaaac aatgaaacaa atgaggtgtt ttcagtcgca aaacataaag gagtaataaa
     3001 tcaaattgaa catttaggtc gttcattttc tgcaaaagaa atttttcact ataaattaat
     3061 ttatccggga gatttagttt atactaaaag ccctacatct gattttcctt ttggaattat
     3121 aaagcaaaat agaacaggtc gaattggtgt tgtttcacca ttatactgtg tatttaaacc
     3181 acaaacttat gctttaggtt atattcttca cgaatatttt aattctgcaa ttaatacctt
     3241 gaattattta agcccattag tacaaaaagg tgctaagaat acaatgaata taaataatga
     3301 tacatttatg aatggtgcaa ggcttttgtt accaacagat gaaaaagaac aagaaaaaat
     3361 tgctaatttt ctatctgcgt tagacgaaaa aataaatcgt acccaaacgc aaatagaaca
     3421 aacagaaagg tggaaaaaag ggttgttgca acaaatgttt gtgtaaacca caatgtatca
     3481 aatggataat tttaaaaata aataccgtat aacgtcggcc cgattacaaa attggggttt
     3541 tggattaaat tccaattccg attccaattc caattgtaga tacgcgatta atcgcgtatc
     3601 tacaattgga atcgccgtca ataatccatt aaattggaat aaagataaat ttcatggcta
     3661 aacaatccga acaaatatta gaagaacaat tagtagtcaa tcttcaaaaa ttgggctatc
     3721 aatacctttc cattaacgat gaaaaagggc tgttgaccaa tctgaaaaat caattggaaa
     3781 aacacaatca aatttcgctt tcagatgctg aatttgataa ggtgctgaat attctcaaca
     3841 aaggttctgt ttttgaaaag gcaaaaacct
//
LOCUS       DAOQVQ010000194         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26944_length_3828_cov_3.380599, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000194 DAOQVQ010000000
VERSION     DAOQVQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26944_length_3828_cov_3.380599"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..727
                     /locus_tag="PKN22_06695"
     CDS             77..727
                     /locus_tag="PKN22_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42429.1"
                     /translation="MLLDSKELGMSDRPVIMAIKGMHGIIDPRFYADPLKWCCSEMIA
                     LQPNEIKKVEVTYPLQPARSFSVENFGNGKYSVKQQNIQLQSIDTQYVMLYLNKFKKI
                     HFEMPNYILNQQQVDSVRRSSPFCILKIDEMNGKSSKFRMFRIPSEEPKINELGQPVA
                     YEIDRFWAELPSGELVKCQYFVFDPLTLGHLYFPLDLRSLDLNNYEVKSPDQYKKD"
     gene            823..1941
                     /gene="dnaN"
                     /locus_tag="PKN22_06700"
     CDS             823..1941
                     /gene="dnaN"
                     /locus_tag="PKN22_06700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNS42430.1"
                     /translation="MDFIISSTTLLRHLQSISGVLSTSNTLPILDNFLFDMEDGRLTI
                     SASDLETTMQTSLEVEANEGGKIAIPAKLLLDVLKNLPDQPCTFSVDYSNFGIEISYD
                     NGKSKMVGFNGEEFPKIPTIENSSSVKVSGDIISSAINKTLFATGNDDLRPVMSGVFC
                     QFTPQDIIFVATDAHKLVRYRRTDSTADSTASFILPKKPLNMLKSNVSSDMEVLIEYN
                     DANAVFTFGDTVLVCRLIDGKYPNYEAVIPKENPNVLVVDRAQFLGSIKRVSIFANKT
                     THQIKLKLAGSELALSAEDIDFANEANERLTCSYSGEDMEIAFNSRFLLEMLNNIETK
                     EVKLEMSIPSRAGLLMPAEVTNPDEDILMLVMPVMLNK"
     gene            complement(2010..3011)
                     /locus_tag="PKN22_06705"
     CDS             complement(2010..3011)
                     /locus_tag="PKN22_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNS42431.1"
                     /translation="MARLSLPSDRFQEQESFYGKRCLHCYEPLKEPNTDVHQACNKRF
                     YGQLKTPELLYDFENLKELATQVIQSQMAVTGVQAKVSLSWYRKAEKNLTKKLTIVGL
                     YGDYILKPPSEFYPHLPELESVSMHMAAKCGLQTVPHSLIYLQDGTLCYITKRVDRTR
                     KGKLHMEDMCQLSERMTEDKYKGSHEQVAKLILNYSNSPLLDVTNFFELVLFSFFTGN
                     ADMHLKNFSLLENPGQGFTLSPAYDLVPTALVNPADTEELSLTLNAKKRKLKYTDFLA
                     AFENCGLSKKVLDNTLELFVYAKPEMLEVLNKSFVSDEYKLKYMSLINARYKQLGLE"
     gene            complement(3022..3348)
                     /locus_tag="PKN22_06710"
     CDS             complement(3022..3348)
                     /locus_tag="PKN22_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNS42432.1"
                     /translation="MQRSGNVYMNNVFCGTISENEDGYFFAYDSDYLSSANAMAVSLT
                     LPLSSKPYTSKTLFAFFDGLIPEGWLLDIAEKNWKINYKDRMGLLLTCCKDTIGAVSV
                     QANEKL"
     gene            complement(3350..3577)
                     /locus_tag="PKN22_06715"
     CDS             complement(3350..3577)
                     /locus_tag="PKN22_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Y4mF family
                     antitoxin"
                     /protein_id="HNS42433.1"
                     /translation="MKENLTELSKHVKLKRKLNRLTQPELALKAGVGLRFVRDLEQGK
                     TTLRMDKVNDVLRLFGETLGPVPMNREDLLK"
BASE COUNT         1242 a          684 c          655 g         1247 t
ORIGIN      
        1 tctccatacc tggaaggact agtaaacgga cgtgtggtat attgggcaag caacaagaga
       61 ccatttaggt caaattatgc tgttagattc aaaagaatta ggaatgtctg atcgtccggt
      121 aattatggct atcaaaggca tgcatggaat tattgaccct cgtttttatg ccgatccttt
      181 aaaatggtgt tgttcagaaa tgatagcatt gcaacccaat gaaattaaaa aagtagaagt
      241 tacctatcca ttgcaacctg cacgtagttt ttcggtggaa aattttggta atggaaaata
      301 cagcgttaaa caacaaaaca ttcaattgca atccattgat acgcaatacg taatgctgta
      361 tttaaacaaa tttaagaaaa ttcattttga aatgccaaat tacattttaa atcaacaaca
      421 agtggatagt gttcgacgtt catcaccatt ttgcatccta aaaatagatg agatgaatgg
      481 caaatcttca aaatttagaa tgtttagaat tccatctgaa gaaccaaaaa taaacgaact
      541 tggtcagcct gttgcctatg aaatagatcg tttttgggca gaattacctt ctggagagtt
      601 ggtaaaatgt caatattttg tttttgatcc attaacattg gggcatttat atttcccgtt
      661 ggatttgcgt tcattggact taaacaacta cgaagtgaag tcacctgacc aatacaagaa
      721 ggattaattt aacaaattgt tgataactaa ggtaaattat tttgcaaaga atttttttat
      781 ttagtatatc atttttagtt ttgaacaaca tttaataaag ttatggattt tataatttca
      841 agtaccacac ttttaagaca tttgcaaagc atttcaggtg ttttaagtac aagcaacacc
      901 cttccaattt tagataattt tctatttgat atggaggatg gtcgtttaac catttcagct
      961 tctgacttgg aaacaactat gcaaacatca ttggaagtgg aagcaaatga aggaggtaaa
     1021 attgcaattc ctgcaaaatt attattggat gtattaaaaa acttacccga tcaaccgtgt
     1081 acattttcag ttgattattc caactttggg attgaaatca gttatgacaa tggtaagtcg
     1141 aaaatggttg gttttaatgg cgaagagttt ccaaaaattc caactattga aaacagtagc
     1201 tctgtaaaag tatctggtga tatcattagc tccgctatca acaaaacatt atttgcaact
     1261 ggaaacgatg atttacgccc agttatgagc ggtgtatttt gtcaatttac acctcaagat
     1321 attattttcg ttgcaacaga tgctcacaaa ttggtgcgtt accgcagaac agactcaaca
     1381 gcagattcaa ctgcttcgtt tattttgcca aaaaaaccgt tgaacatgtt gaaatcaaac
     1441 gtttcatcgg atatggaagt attgattgaa tacaacgatg caaatgctgt tttcacgttt
     1501 ggtgatacag ttttggtgtg ccgcttgatt gatggaaaat atccaaacta cgaagccgtt
     1561 attccaaaag aaaaccctaa tgtgttggta gttgacagag cacaattctt aggttctatc
     1621 aaacgtgtat ccatctttgc taacaaaaca acgcatcaaa tcaaattaaa attagctggt
     1681 tctgaattag cattatcagc agaagatatt gattttgcaa acgaagcaaa cgaacgttta
     1741 acttgtagtt acagcggtga agatatggaa attgctttca actctcgttt cttattggaa
     1801 atgttgaaca acattgaaac aaaagaagtg aaattggaaa tgtctattcc aagtcgtgct
     1861 ggacttttaa tgccagcaga agtaaccaat ccagatgaag acattttgat gttggtaatg
     1921 ccagtgatgt tgaataaata accaaaaaaa tcatagaaaa agccagattg aatgctcaat
     1981 ctggcttttt tattttgtag aatatttatt tattccaatc ctaattgttt gtacctcgcg
     2041 ttgattaaag acatgtattt aagtttgtat tcatcgctaa caaagctttt gttgagaacc
     2101 tccaacattt ctggttttgc ataaacaaaa agctctaagg tgttatccaa aacttttttg
     2161 ctcaaaccac aattctcaaa agcagccaaa aaatcggtgt attttagctt tcttttttta
     2221 gcgttgaggg ttaaagataa ttcttcggta tcggctgggt ttaccaatgc cgtaggcacc
     2281 aaatcatagg ctggagaaag tgtaaaacct tgccctggat tttcaagcaa cgaaaaattt
     2341 ttgaggtgca tgtctgcatt tccagtaaag aaactaaaca acaccaattc aaaaaaattg
     2401 gtaacatcca acagtggcga attggaataa ttgaggatta gcttagcaac ttgctcatgg
     2461 ctacctttgt atttatcctc tgtcattcgc tcgctcaatt ggcacatatc ctccatgtgt
     2521 aatttgcctt tacgtgttct atccactctt tttgtgatgt agcacagcgt tccatcctgc
     2581 aaataaatta aactatgcgg aacagtttgc aaaccacatt ttgctgccat gtgcatagaa
     2641 acgctttcta gttcgggcaa atgagggtaa aactcagatg gtggtttgag gatgtaatct
     2701 ccgtatagac caactattgt tagcttttta gttaaattct tttcggcttt tctataccaa
     2761 ctcaacgaca ccttagcttg cacaccagta accgccattt gactttggat cacctgagta
     2821 gcgagttctt ttaggttttc gaaatcatag agcaattcag gtgttttcaa ttgcccataa
     2881 aaccgtttat tgcatgcttg gtgcacatct gtattgggct ctttaagtgg ttcgtagcaa
     2941 tgcaagcatc ttttaccata aaagctctcc tgctcctgaa aacgatcaga aggaagcgat
     3001 aaccgagcca aatagttttt tttaaagttt ttcatttgct tgcacactaa cagcaccgat
     3061 ggtgtccttg caacatgtta acagcaaacc cattcggtct ttataattta ttttccaatt
     3121 tttctctgcg atatccaaca gccaaccctc cggtattaag ccatcaaaga atgcaaaaag
     3181 cgttttactt gtataaggtt tcgatgaaag tggtaatgta aggctaacag ccattgcgtt
     3241 tgcactagaa agatagtcgc tatcgtatgc aaaaaagtaa ccatcttcat tttcagagat
     3301 agtgccacaa aacacattgt tcatatatac atttccactt ctttgcatct tacttcaata
     3361 aatcttctct attcatagga acggggccta atgtttctcc aaacaaacgc aacacatcgt
     3421 ttactttatc cattcttaat gtagttttac cttgttctaa atcgcgaaca aatcgcaacc
     3481 caacaccagc ttttaatgcc agctctggtt gagtaagacg atttaacttg cgtttaagtt
     3541 tgacgtgttt acttaattcg gttagatttt ccttcatttt tatacctttt acactacaaa
     3601 tatacatttt tcctgatatt taatactaca ttgggtatat tttaacaaaa aacacctaat
     3661 cctataccct tttgagtata tttttaaaga ttgctataaa ataaaagtcc gaaaataaac
     3721 tattttcgga cttttaaagt agcatatagt agtacctaaa acactaataa atcattaaat
     3781 tcaagttata aatgcaactt ttagatttta cgtcaaaata acttcaaa
//
LOCUS       DAOQVQ010000195         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_26963_length_3826_cov_3.903209, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000195 DAOQVQ010000000
VERSION     DAOQVQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26963_length_3826_cov_3.903209"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PKN22_06720"
     CDS             complement(<1..1724)
                     /locus_tag="PKN22_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42434.1"
                     /translation="MFFSVFSVWGQDKESIVQQRIEFISEQLETEELDLTDVIQTLYF
                     RYDNPLNLNNCTKEDLEELNLLTDIQVNDLLLHRQKFGKLINIYELQSLEFWSLETIY
                     LVLPFVRVEDRTDQLHVSFKEAIKRGKFEWYARYQRVLEAKKGYQAPNDSVLKASNSY
                     YHGNPDKYYTRFRYSYRTNISIGLTAEKDPGEQFFKGSQKQGFDFYSAHAFYKGGKYL
                     RAVAIGDFQTQIGQGLNMWTGYAFGKTADVVNTKKSAIPLKPYTSVDENRFLRGAGVD
                     LGYKDFSLTLFGSVKTVDGATTTVDTSSTDDSDFFVPEETVSSINTTGFHRTNSEISR
                     KGSLTEIIYGANFRYNHKNLHIGAAAVNWGYNKPYVKPVEPYNQFDFRGKNTTSISVD
                     YNYVIRNFNFFGEVSYVTHSNSWATLHGVVMALHSRVSMSLVYRHYQRGYSTFYNTAF
                     AASGRTENATNESGLYMGLSVGITKTFGINMYFDYYKFPWMKYQVNQPSYGHDLLIQP
                     TFKPSKSLEIYARFRQHLRQKNSRNSDGTIIGIEDVYQRNYRLNVTYKVSEDFTLKSR
                     VEYVTINRP"
     gene            complement(1741..2568)
                     /locus_tag="PKN22_06725"
     CDS             complement(1741..2568)
                     /locus_tag="PKN22_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42435.1"
                     /translation="MKIILLFTTLLLQSIVFSQGWVPVGSRSNALANASVTLVDAWAY
                     HHNPGALGEITSPTVGISYENRFLLKEFQSQGIAYAHPLKYGVISVGGQFYGYKLFRT
                     QRVGVGYSLKLAKRFYAGVQINYQGLQLNSNYGNKNTVTGEIGLQALLTDNWRLGFSV
                     LNVGATKLNSYQNERFATHFRLGMSYLLANKVLFLLEGSKTIVDKVKIKGAVEYEPFK
                     NFFIRAGVASAPLEITFGFGYKWKVVSFDIGSAYHNVLGWSPNISITYSANKKQHVD"
     gene            2736..>3826
                     /locus_tag="PKN22_06730"
     CDS             2736..>3826
                     /locus_tag="PKN22_06730"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HNS42436.1"
                     /translation="MFQKVKYIVYSLMVAPFVLASCTTSENNQTNHKVHVARVGDIHS
                     YANLDKIRTKHLHLELDVNFPNKTIYGIARHQMERLKKTDTAIFDIKYLNIQKVTTGK
                     GNEKDADYVIGETDSILGAPLLVAIDSTVEYINIYYKTTEKTEALDWLDPELTEGKKH
                     PFLYSQGESILTRTWLPVQDSPENRITYSADVKVPKEYLAIMSAENPQKNNKTGLYHF
                     EMKQPIPAYLIALAVGNIEFKSIGENCGVYSEPELLDACANEMVDLPKMIQAAENQFG
                     KYQWGRYDVVVLPYSFPFGGMENPRLTFANPTLITGDRSLVTVIAHELAHSWSGNLVT
                     NASWEDFWLNEGFTVYLENRIMEQLYGKDV"
BASE COUNT         1233 a          734 c          669 g         1190 t
ORIGIN      
        1 ggtcggttaa tggttacata ctctacccta cttttgagcg taaaatcttc cgaaactttg
       61 taggtaacgt tcaatcggta attgcgttga tatacatctt ctattcctat gatagttcca
      121 tccgaattgc ggctattttt ttgacgcaag tgttggcgaa aacgtgcata tatctccaac
      181 gatttagaag gtttaaaagt aggttgaatc aacaaatcat gaccataact aggttgattt
      241 acttggtatt tcatccatgg aaatttgtaa taatcaaaat acatgttgat gccaaatgtt
      301 ttggttattc ctacgctcaa tcccatgtat aaaccacttt cattggttgc attttcggtt
      361 cttcctgatg ctgcaaaagc ggtattgtaa aacgtactat aacctcgttg ataatgacgg
      421 taaacaagac tcattgaaac acgcgaatgc aatgccatca ccactccgtg caatgttgcc
      481 cagctgttgg aatgtgtaac ataactcacc tcgccaaaaa agttgaaatt gcgaatcaca
      541 taattgtaat ccaccgaaat ggaagtagta ttttttcctc taaaatcaaa ttgattgtaa
      601 ggttctactg gttttacata gggtttgttg tacccccaat tcactgctgc cgcaccaatg
      661 tgcaagtttt tatgattgta acgaaagtta gctccataaa tgatttcggt taatgagcct
      721 tttctactaa tttcagagtt agtacggtga aaaccagttg tattgatact tgaaacggtt
      781 tcttcgggta caaaaaaatc agaatcatcg gttgaagacg tgtcaactgt tgtggttgca
      841 ccatctaccg tttttacaga accaaatagg gtgagtgaaa aatctttgta gcccaaatca
      901 acccctgctc ctcttaaaaa tcgattttca tccaccgaag tatagggttt taatggtatg
      961 gcgcttttct tcgtgttcac cacatcggct gttttaccaa aagcatatcc cgtccacatg
     1021 tttaaaccct gaccaatttg tgtttgaaaa tcgccaatgg caacagcacg caagtatttt
     1081 ccgcctttgt aaaaagcatg tgccgaataa aaatcaaatc cttgtttttg agaaccttta
     1141 aaaaattgct cgcccggatc tttttcagct gttaaaccaa tgctgatgtt ggttcgatac
     1201 gaataacgaa aacgggtata atatttatcg ggattgccgt gataatacga attactggct
     1261 tttaaaacag aatcattggg tgcttgatag cctttttttg cttctaaaac gcgttgataa
     1321 cgtgcatacc actcaaattt tcctcgcttg atggcttctt taaatgaaac gtgcaattgg
     1381 tcggtgcgat cctccactct cacaaaaggc aacaccaaat aaatggtttc caaactccaa
     1441 aactccaaac tttgcaactc gtagatgtta atcaacttgc cgaatttttg gcggtgcaac
     1501 aacaaatcat tgacttgtat atcggttaac aaattcaatt cttccaaatc ttctttggta
     1561 cagttgttta aattcaacgg gttatcatat cgaaaataaa gtgtttgaat cacatcggtc
     1621 aaatccaatt cttcggtttc taactgttct gaaataaact caatgcgttg ttgcacaata
     1681 ctttctttgt cttgccccca aacagaaaag acactaaaaa acattatgaa cgcaatgaat
     1741 ttaatccaca tgttgttttt tgtttgcaga ataagtgatt gaaatgttag gcgaccaacc
     1801 caagacgttg tggtaggcag aacctatatc aaacgaaacc actttccatt tgtatccaaa
     1861 tccaaacgtt atttctaacg gagcacttgc aacacctgcg cgtataaaga aattcttaaa
     1921 cggttcgtat tctaccgcac ctttgatttt aactttatcc acaattgttt tagagccttc
     1981 taataaaaac aagactttat tggctaataa ataactcatt cccaacctaa aatgcgtagc
     2041 aaaacgttca ttttggtaag aatttaattt cgttgctccg acattaagaa cactaaaacc
     2101 caaacgccaa ttgtcggtta acaatgcttg caagccaatt tcacccgtca ccgtgttttt
     2161 attgccataa ttggaattca attgcaaacc ttggtaatta atttgcacac ctgcatagaa
     2221 acgtttggcc aattttaagc tgtaaccaac tcctacgcgt tgcgttcgaa acaatttata
     2281 gccgtaaaat tgtccaccaa cagaaataac accatatttt aaagggtgtg cataagcaat
     2341 tccttgactt tgaaattctt taagtaaaaa acgattttcg taacttatac caactgttgg
     2401 actggttatt tcgcctaaag caccaggatt gtgatgatag gcccaagcat ctaccagcgt
     2461 taccgaagca tttgccaaag cgttggagcg agaaccaaca ggaacccaac cttgcgaaaa
     2521 tacgatagat tgcaaaagaa gcgtagtaaa cagaagtata attttcattt agttcttttc
     2581 tatttcttac aaatctataa aaaaagcaat atcaacttaa caaacgctaa agtttattta
     2641 ttctcatcaa ttgtttataa gtctgaaaaa tagtcgaaaa aacgatttat ttatcatttt
     2701 ttatttttat atatttggca actttaattt taaacatgtt tcaaaaagta aaatacattg
     2761 tttattcatt gatggttgcg ccttttgttt tagcatcttg cacaacatct gaaaataatc
     2821 aaaccaatca taaagtacat gtggcacgag tgggcgacat acattcgtat gccaatttgg
     2881 ataaaattag aaccaaacat ttgcatttag aactcgatgt caattttccg aataaaacta
     2941 tttatggcat tgctcgacac caaatggagc gattgaaaaa aaccgatact gccatttttg
     3001 acattaaata cctgaatatc caaaaagtta caacaggaaa aggcaatgaa aaagatgccg
     3061 attatgtgat tggtgaaacc gattctattt taggagcccc cttgctagtt gccattgatt
     3121 ctactgtaga atacatcaac atttactata aaacaaccga aaaaacggaa gcattggatt
     3181 ggttggatcc tgaattgaca gaaggaaaaa aacatccttt cttatactcg caaggcgaaa
     3241 gtattttgac acgtacttgg cttcctgttc aagatagtcc tgaaaacaga attacctatt
     3301 ctgcggatgt caaagtacca aaagaatatt tggccatcat gagtgccgaa aatccacaaa
     3361 agaataacaa aactggtttg tatcattttg aaatgaagca acctatacct gcttatttaa
     3421 ttgcacttgc tgttggtaac atagaattta aaagtatagg cgaaaattgt ggggtgtatt
     3481 cagaacctga attattggat gcttgtgcca atgagatggt ggatttacca aaaatgattc
     3541 aagccgctga aaatcaattt ggaaaatacc aatggggacg atacgatgtg gtagttcttc
     3601 cctattcttt tccgtttgga ggaatggaaa atccacgttt aacgtttgca aatccaacgc
     3661 taataactgg tgatagatca ttggtaacag ttattgcaca cgaattagca cattcatggt
     3721 cgggcaattt ggttaccaac gcttcgtggg aagatttttg gttaaacgaa gggtttaccg
     3781 tttatttgga aaaccgcata atggagcaac tttatggaaa agatgt
//
LOCUS       DAOQVQ010000196         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27101_length_3811_cov_4.272630, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000196 DAOQVQ010000000
VERSION     DAOQVQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27101_length_3811_cov_4.272630"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKN22_06735"
     CDS             <1..715
                     /locus_tag="PKN22_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNS42437.1"
                     /translation="DLNEASPKLVIEANYIATDSLVRVKVSYTGNYFDAYSSNLVNNA
                     VISIKASNGASVSVPFIGNGMYELANYAPTYGATYTIQVLHEGIAYEASSLLMPTMDF
                     TPASYEYQEETLFSEAGYWVTYRFQDPPGIGNCFKIIETYNDTTYNKFGDFRFGDDKN
                     TDGNIRELKIYEAFAIGDTIELELQSINQSVFNYYDQLSTNTSAFTAAQGNPDYYWTN
                     EALGYFSAYGYKKQQIVIQ"
     gene            complement(766..2385)
                     /locus_tag="PKN22_06740"
     CDS             complement(766..2385)
                     /locus_tag="PKN22_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42438.1"
                     /translation="MKRITLSVFSLVALTGMSFGQTLGDAIKKTDNERYEEAAKDFRS
                     LAKQQGISLGDVYFFYGDNFYQADDVDSAKIIWNKGAEVAPENPLSLVGKAKAMWVSG
                     DTTNASTLFQTILKDTKRKNAEVIRQIAAVMVESKIKSLNQAVGLLNKAIELEPKNQR
                     NYLLMGDAQVEINPRNSTEAMKYYNKAGDINSNARVFVSKAKIYQRAQNPKLADSLYK
                     VAQSMEPNYAPAYRERAELNMRFGQTDQSISNWKKYLELNDSDHARYRYATSLFLGKK
                     YSDAIKEIDLLHSRGFKNMYAERILAYSIYEDNAANNADSSAYVVGLNALNNYFKMAP
                     KDKVIGSDYRYEGLYYQKLNQPAKYFECMQKAAEIDPEVRGDIYGELAATYMKDKNYQ
                     GAIDMFTKKMGSDSSNLTLAEYYELGRAYYFGPKDYAKADQAHAKVLEQAPTYAMSYL
                     WRARSNSYLDENKTTWAAKTYYDGFLANLSDDQIKGQFKSMAIEAYKYLGDYYVNSAD
                     KNMDKAKEVWGKVQALDPNDAQAKGFFKTVQ"
     gene            complement(2493..3413)
                     /locus_tag="PKN22_06745"
     CDS             complement(2493..3413)
                     /locus_tag="PKN22_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNS42439.1"
                     /translation="MKQVILFFSVLIALSSCKNSMQGKNTNSSGDIVVYLEESFKPLF
                     ETSIYTFEGQYPLANILPQYVNETAAIDAFMNRKTSTICITRDFTKEEKDALRKQNNI
                     EVRSTKMAIDALALVVHPENADTLMTVDRLKNILQGKDSLWTGTKNVINVVFDNPNSA
                     NFLYLKNLAQVEQMPVNVFAVNSNEEVINYVKSHKNALGVIGVNWISDEEDPQALKFR
                     DGLNVVSIAKSEGEEYFKPYQAYIYEGYYDNKAYPLVREVWIITKAGRSTLDAGLVNF
                     MVGEKGQLIILKSSLIPADMQARMLNIRTE"
BASE COUNT         1176 a          747 c          601 g         1287 t
ORIGIN      
        1 tgatttaaac gaagctagtc ccaaattggt gattgaagcc aactacattg caaccgatag
       61 tttggtgagg gtgaaagttt cctataccgg caattatttt gatgcctatt cttctaattt
      121 agtcaacaac gctgtaattt ctattaaggc aagcaatgga gcttcggttt ctgttccttt
      181 tattggcaac ggaatgtatg aactagccaa ctatgctcca acttatggcg ccacttacac
      241 gattcaagtt ttacacgaag gcattgccta cgaagcatcc tcgttgttaa tgcctacaat
      301 ggattttaca cctgccagct atgaatacca agaagaaaca ttgttttctg aagcaggtta
      361 ttgggtaacc tatcgttttc aagaccctcc aggaattggc aattgtttta aaatcattga
      421 aacttataac gatacaacct acaataaatt tggagatttc cgatttggtg acgataaaaa
      481 caccgatggc aatatccgtg aattgaaaat atacgaagca tttgcaattg gcgatacaat
      541 agaactggaa ttgcaaagca tcaatcaatc ggtttttaat tattacgatc aattatccac
      601 caacacatcg gcatttacgg cagctcaagg aaacccagat tattactgga ccaacgaagc
      661 attgggttat ttttcagctt atggctacaa gaagcaacaa attgtgatac aataaaaaag
      721 acgagctaat aactaactcg tctttctcaa taattttaaa aagaattact gcactgtctt
      781 aaagaatcct tttgcttgtg catcgtttgg atctaaagct tgcactttac cccacacttc
      841 ttttgcttta tccatgtttt tatcagcaga gtttacatag taatcgccca aatatttgta
      901 agcttcaatt gccatgcttt taaactgacc tttaatttgg tcgtctgata aatttgctaa
      961 aaacccatca taatatgttt ttgcagccca tgttgtttta ttttcatcca agtatgaatt
     1021 agaacgtgct ctccacaaat aactcatcgc ataagttggt gcttgctcca acacttttgc
     1081 atgtgcttgg tctgcttttg cataatcttt tggaccaaaa taatatgctc tacctaattc
     1141 ataatattct gccaaagtca agttagatga atcacttccc atttttttag tgaacatatc
     1201 aattgcacct tggtagtttt tatctttcat ataagttgca gccaattcac cataaatatc
     1261 tcctctaact tcaggatcaa tttcagcagc tttttgcatg cactcaaaat atttagctgg
     1321 ttgatttaat ttttggtaat acaacccttc gtaacggtaa tccgaaccaa taactttatc
     1381 ttttggagcc attttgaaat aattgttcaa ggcattcaaa ccaacaacat aagctgatga
     1441 atctgcattg ttagccgcat tatcttcgta aattgaatat gctaaaatac gctctgcata
     1501 catgttttta aatcctcttg aatgcaacaa atcaatttct ttaattgcat cactgtactt
     1561 tttacctaag aataacgatg tagcataacg gtaacgtgca tgatcactat cgtttaattc
     1621 taagtatttt ttccagttgc tgatggattg gtctgtttgt ccaaaacgca tgttcaactc
     1681 agctctttcg cggtatgcag gtgcatagtt aggctccata gattgtgcaa ctttgtataa
     1741 actatctgcc aatttagggt tttgggcgcg ttggtaaatt ttagccttgc taacaaaaac
     1801 acgagcattg ctattgatat cacccgcttt gttgtaatac ttcatagctt ctgtactgtt
     1861 tcttggattg atttccactt gcgcatcccc catcaacaaa tagttgcgtt gatttttcgg
     1921 ctccaactca attgctttat tcaacaaacc tacagcctga ttcaaacttt tgattttaga
     1981 ctctaccatt acagctgcaa tctgacgaat aacttctgcg tttttacgtt tggtatcctt
     2041 taaaattgtt tgaaaaagtg tacttgcatt ggttgtatca ccagaaaccc acattgcttt
     2101 tgcttttcca actaatgaca atggattttc gggcgcaact tcagcacctt tattccaaat
     2161 aatttttgca gaatcaacat cgtctgcttg gtaaaaatta tctccgtaaa agaaataaac
     2221 atcgcccaaa gaaatacctt gttgttttgc taaagaacga aaatcttttg ctgcttcttc
     2281 ataacgttcg ttgtctgttt ttttgatagc atcacccaaa gtttgtccaa aagacattcc
     2341 tgttaatgct actaatgaaa acactgataa tgtaattcgc ttcattttat ttaattttct
     2401 agttacctat tttttcaagt ttgaaaaatc aaaattcttg ccaaaagcaa aagttaattt
     2461 taaaacggtt gctaatatac tatttttttt gcttattctg ttcggatatt caacatacgt
     2521 gcttgcatat ctgcaggaat taaagacgat ttaagaataa ttaattgtcc tttttctccc
     2581 accataaaat tcactaaacc agcatctaaa gtagaacgac cagctttggt gattatccac
     2641 acttcgcgca ctaaaggata ggctttgtta tcgtaatacc cttcgtaaat atatgcttga
     2701 tacggtttga aatactcctc accttctgat tttgcaatgg aaacaacgtt caatccatct
     2761 ctaaacttta atgcttgcgg gtcttcttca tcactgatcc aattcacacc aatgactcct
     2821 aacgcatttt tgtgcgattt tacatagttg ataacctcct cgtttgagtt cacagcaaac
     2881 acgttgacag gcatttgttc cacttgagcc aagtttttca agtacaagaa attggcagaa
     2941 ttcgggttat caaagacaac attaatcaca tttttggtac ccgtccacaa cgaatctttt
     3001 ccctgaagaa tgtttttcaa tcggtcaaca gtcatcaatg tatcggcatt ttctggatgc
     3061 accaccaatg ccaaggcatc aattgccatt ttggtacttc taacctcaat gttattttgt
     3121 ttgcgcagag cgtctttttc ttctttagta aaatcgcgcg taatgcagat tgtagatgtt
     3181 tttctgttca taaaagcatc aattgctgct gtttcattaa catattgcgg aagaatgttt
     3241 gccaacggat attgcccttc gaaggtataa atactggttt caaacaaagg cttaaacgat
     3301 tcttcaagat atacaacaat atctccagag ctgttggtgt tttttccttg catgctgttt
     3361 ttgcacgatg ataatgcaat aaggacggag aaaaataaaa ttacttgttt catatcgatt
     3421 aattttcaga ttctttagaa tttttatcgg ctttatatag gttgtaaacg cgatatgagc
     3481 gaaaaagtgc atacaacaac aataaaacaa tgaaagattt acgttgaaaa ttggataaat
     3541 tcattaaaaa ctcctcctta aaaagaagaa aaaagcacca aaccaagcaa attacgataa
     3601 caaatgctga gattaggtaa ttgataattc ttgcgttcac tttaaaatat ttcttcaaaa
     3661 ataatcaata tcaattggaa atggaaaact ctttgttaag aattgtcaaa agaaagaaaa
     3721 aaaggcaaat ctataagccg ggttctgtat tctaaaacta gaacgtttat catttatcta
     3781 gccgcacaat cacttgtacg atctatcaac c
//
LOCUS       DAOQVQ010000197         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27280_length_3793_cov_3.760835, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000197 DAOQVQ010000000
VERSION     DAOQVQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27280_length_3793_cov_3.760835"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..751)
                     /locus_tag="PKN22_06750"
     CDS             complement(224..751)
                     /locus_tag="PKN22_06750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNS42440.1"
                     /translation="MGLVTLLLFPIPAFVTLHFLEGTTFHDFLFSTPFDWDVFLIGIL
                     VGCVYAIAANKLMQLPVFDNTPLKIDELVNSLNLTIADALFLSLCAGIGEELLFRVGF
                     QHYFGVILTSVFFVAIHGYLNPFNWRHSLYGIIVLPLSFLLGWGYELLGLWFAIAVHT
                     SYDFTLFIAFSKNKR"
     gene            891..2180
                     /locus_tag="PKN22_06755"
     CDS             891..2180
                     /locus_tag="PKN22_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNS42441.1"
                     /translation="MKESIINWIKSIYEHTINGEKVFNSELYASHTILWSLGMFAVLG
                     ISFLIFWFLFKLVLVKITHSYFHKSKVKWDDYLVKNKVFKAIAYLIPSLFLDFFFSIV
                     FYEYPSVYSVFSMLSKLVIVFIFIVVVNRILVTIKDILLDNVKFKDKPVQSYIQVIQI
                     IVTIVLIAVMISILTGVKPINLLASFGAASAILMLVFKDTILGFVGSIQLGGNDIVRI
                     GDWITMEKFGADGTVEEINLTTVKVRNFDKTITTIPTYSMVSDSFKNWRGMEESDGRR
                     IMRSVKININSIKYADQELIEKLKKFIILKDYLESKQAEIDQYNQKNGLIGEYAIGGR
                     RQTNIGIFRKYMEFYITNKPEINKNMPILVRQLESTDTGLPIQIYCFALAKTSVDYEP
                     IQSDIFDHIFAVIHEFELNVFEQPSGNDMKIWNREKN"
     gene            2486..3067
                     /locus_tag="PKN22_06760"
     CDS             2486..3067
                     /locus_tag="PKN22_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42442.1"
                     /translation="MMIISLSSKELYAQNNYSPAKQVSNVLVNSNLESKYLWEKEAFG
                     NRAQSSEIIELRDANSKHFKNENGDVTAFITAGNINYLENGKWKTIFHSIVSDANGFS
                     NTTNFHKTYYPQTAAGAITTILPSGETLVDMKDMRMYYLVGNSVVQPLTIAPNTGTVD
                     FNELTYSNVYGNGIDLKLTQHTTSEKWIISFKI"
     gene            3043..>3793
                     /locus_tag="PKN22_06765"
     CDS             3043..>3793
                     /locus_tag="PKN22_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42443.1"
                     /translation="MDYIIQNLNALATIPTGAEYLVFEEKVVLPNGWNATLTDENYIL
                     VTGTNGLKAVFNKPVFIESPSMHTYEVNGESHTHSGAERIDGTYQLEQNGTVLTIKTL
                     VPIAWLTAAERTFPVTIDPSVNMYPNNANWWTGTIHTYGNTPTVYTETDIYDAYDDEM
                     IIGKGNYDPWYGYYESDDCYHSWAKFNLTTLPSTCLSTVSFHFYTADDAGVIGSGCFM
                     NFNVKPLAYDPVPSSNATVLADIRDGSLYGSF"
BASE COUNT         1327 a          570 c          641 g         1255 t
ORIGIN      
        1 aatgtagcgt taagcgtgtt gtaacgttct acagcacgag ttccagctgc agccaaatcc
       61 tgcgccataa ctaattggca agcaaaagca aaaccgaatg ttaaaataaa atttttcata
      121 ttaataattt tcaacttttc ttacgaatat tgtaccaatg tactttcgta tatatgacga
      181 caaagttaca aaaaagttgc tatgttgcat atttctttaa ttttcagcgt ttatttttac
      241 taaaagctat gaataacgtg aagtcatagc tagtgtgcac cgcaattgca aaccataacc
      301 ccaacaattc atacccccaa ccaagcagaa aagaaagtgg taaaacgatg atgccatata
      361 gagaatgtct ccaattaaat ggatttaagt atccgtgtat ggcaacaaaa aataccgagg
      421 ttaaaataac tccaaaataa tgttgaaacc caactctgaa gagcaattct tcaccaatac
      481 cagcgcaaag ggataaaaat aaagcatcgg cgatagttaa attcaatgag ttaactaatt
      541 catcaatttt taatggagta ttgtcaaaaa cagggagttg cattaattta ttggcagcaa
      601 tggcataaac acatcccacc aatattccta ttaaaaacac atcccaatca aatggagttg
      661 aaaataaaaa atcgtggaaa gttgtcccct ccaaaaaatg cagtgtaaca aatgcaggga
      721 ttggaaacaa caataatgtt accaagccca tcaagtatat gtatttttta ttcaacgtgt
      781 gagtttgtgt gaatggaagc tttaagtagt ccactaattt agagcaaaaa tatacgaata
      841 caattaaata gccaactttt aatttttatt tttgcaaaaa cttaatttgc atgaaagaat
      901 caataatcaa ttggataaaa agtatttatg agcacacaat aaatggcgaa aaagttttta
      961 atagcgagtt gtatgcttct cacacaatct tgtggagttt aggaatgttt gcagttttag
     1021 gtatttcatt tttaatattt tggttcttat ttaagttggt tttagttaaa attacccatt
     1081 catattttca caaatcaaaa gtaaaatggg atgactattt ggttaaaaac aaagtgttta
     1141 aggctattgc ctatttgata ccttcattgt ttttagattt tttcttttcg attgtattct
     1201 acgaatatcc aagtgtttat agtgtatttt caatgctatc taaacttgta attgttttta
     1261 tttttattgt tgtagtaaac agaattttag taactatcaa agatatatta cttgataatg
     1321 taaaatttaa ggataaaccg gttcaatcct atattcaagt aattcaaatt atagtaacca
     1381 ttgtattaat tgcagtgatg attagtattt taactggtgt gaaaccaatt aatttattgg
     1441 catcttttgg tgctgcttct gcaattttaa tgctcgtttt taaagataca attcttggtt
     1501 ttgttggtag tattcaattg ggagggaatg atattgttcg aataggcgat tggattacga
     1561 tggaaaaatt tggggcagac ggaacagtag aggaaatcaa cttgacaaca gtaaaagtta
     1621 gaaattttga taaaacaatt accacaattc caacctattc aatggtttcc gactcgttta
     1681 aaaactggcg aggaatggaa gaatcggacg gtagaagaat catgcgctcg gtgaaaataa
     1741 acatcaattc cataaaatat gccgaccaag aattaatcga aaaattgaag aaatttatca
     1801 ttcttaaaga ttatttggaa tcgaaacaag cagaaattga tcaatacaac caaaaaaatg
     1861 gcttaattgg tgaatatgca attggtggaa gaagacaaac gaatattggg atttttagaa
     1921 aatacatgga attctatatt accaataagc cagaaattaa taaaaacatg cccattttgg
     1981 tgcgccaatt agaatctact gatacaggtc tgcctattca aatttattgt ttcgccctcg
     2041 cgaaaacaag tgttgattac gaacctatac aatccgatat ttttgaccat atttttgctg
     2101 ttattcatga gtttgaatta aatgtgtttg aacaaccttc tggaaatgac atgaaaatat
     2161 ggaatagaga gaagaattga tttgttaatt aaatgaatcg ctaaagaaaa tatgttaaaa
     2221 aaataacatt gtttctaatg atttatttcc tcttttttcg atgaaagccc tttatttttc
     2281 gatgaattta ttgaataatt tcataataag atgtttttat cttgtattta attgatagat
     2341 agtgttttat tttttttcat ttatatttga caaaaaattg ataaaccatt aattattaca
     2401 ctatttacac tatatgaaaa agatgtataa ctgctataga ctacgctgta ttcctttttt
     2461 tgcaatgact aaaattatcg tttttatgat gattattagt ttgtcttcca aagaactata
     2521 tgcacaaaac aactactcgc cagcaaagca agttagtaat gttttagtaa attctaattt
     2581 agaatcgaag tatttatggg aaaaggaggc atttggaaac agagcacaat cgtcagaaat
     2641 tattgaatta cgcgatgcca actcaaagca tttcaaaaat gaaaatggag acgtaacagc
     2701 ttttattaca gctggtaaca ttaattattt agaaaatgga aagtggaaaa ctatttttca
     2761 cagtattgtt tcggatgcca atgggttttc caatacaacc aattttcata aaacatatta
     2821 tcctcaaaca gcagcaggtg ctatcacaac aattttacca agtggtgaaa ccttggtgga
     2881 tatgaaggat atgcgcatgt attatttagt tggtaattct gtagttcaac ctttaactat
     2941 tgcacctaat acaggaactg ttgattttaa tgagttgacc tattccaacg tttatggaaa
     3001 tggaattgat ttaaaactta cgcagcatac cacctccgaa aaatggatta tatcattcaa
     3061 aatttgaatg ctttagctac tattcctact ggtgcagaat acttagtgtt tgaagaaaaa
     3121 gtagttttgc caaacggttg gaacgctact ttaaccgacg aaaattacat tttagttaca
     3181 ggaacaaacg gattgaaagc tgtatttaac aaacctgttt ttattgagtc accttctatg
     3241 catacctatg aagttaatgg agaatcacac acgcacagtg gggcagaaag aatagatggt
     3301 acttatcaat tggagcaaaa tggcacagtt ttaaccatta aaacattggt accaatagct
     3361 tggttaactg ctgcagaacg cactttccca gtaaccatcg acccttcagt taatatgtat
     3421 cctaacaatg caaattggtg gacaggtaca atccatacat acggtaatac accaactgta
     3481 tatacagaaa cagatattta tgatgcatac gatgatgaaa tgattattgg aaaaggaaat
     3541 tatgatccat ggtatgggta ttatgagtct gatgattgtt atcactcttg ggcaaagttt
     3601 aacttaacta cattgccgtc aacttgcttg agtactgtta gttttcattt ttataccgct
     3661 gatgatgctg gagttattgg ttcgggttgc tttatgaatt ttaatgtaaa accgttggcc
     3721 tatgatcctg tgccttcttc aaatgctact gtattagcag acattcgtga tggttctctt
     3781 tatggttctt tta
//
LOCUS       DAOQVQ010000198         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27336_length_3789_cov_3.622389, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000198 DAOQVQ010000000
VERSION     DAOQVQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27336_length_3789_cov_3.622389"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PKN22_06770"
     CDS             <1..936
                     /locus_tag="PKN22_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNS42444.1"
                     /translation="YTYWNDVYKGEKGDLPLSFYVLKNNLKVAQTHFLEVYRMLEAFE
                     DWLGPYPFYEDGYKLVQVPYLGMEHQSAVSYGNGFTNGYLNSDLSETGWGMNWDFIII
                     HESGHEWFGNSITSSDIADMWIHEAFTTYTEEIFTEYYWGPTAGDEYIRGIRHRILND
                     EPIIGKYNVQYSGTSDMYFKGANLLHTIRQIVNNDAKWKQFFRDFNLVFRHKITTTED
                     VEKFMSAYFNIDLTSVFNQYLRTTNVPSLYLKKKGKYWVYKWNNVVNDFTMPLHVMVD
                     DTLQTIVPTLKQQKLKAKCIAINNNYYIKVNGRFF"
     gene            complement(1075..2304)
                     /locus_tag="PKN22_06775"
     CDS             complement(1075..2304)
                     /locus_tag="PKN22_06775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNS42445.1"
                     /translation="MKTRHQLFIDNYIVKPFVYLLNFLVRLVGKLLSIDHSLDRDFKR
                     IVICKFKGMGSIIQATPMIEAIRSRYPEAEITFVSTQSNRAILQKIKTINHIVTINDK
                     NVFILSKTVLQALFKLIRHRPEVYIDLEIYSNFSTLFTLLTFSKNRIGFYLRSSSFRM
                     GIYTQMMFFNVKAPISEVYMQIARLFGKPAIAPKLFPIYTNIQVSNFDFPKEKYILIN
                     PNASDLRIERRWGRKKFKELIHLINDTFPQYKVLIIGSKNEWQYSQYVTENTNSQLTQ
                     NIAGKTSIDELIYLIKNATLMVTNDTGPMHIGFACETPSICLFGPCSPDQYGWTSNVE
                     IIYKNVFCSPCVHDFNIAPCNGEKTCMKLITVEEVFEAVKKRLNGEANTKDSNKKSPI
                     IYHTEYTPSLGLVKRTL"
     gene            complement(2294..>3789)
                     /locus_tag="PKN22_06780"
     CDS             complement(2294..>3789)
                     /locus_tag="PKN22_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42446.1"
                     /translation="LPIMLVLSNEKINYQKIAKAVVFLLALLVSMLGIALLLRNGSYI
                     YDNFMAALHYVKANQAHGYTSIANSYPQQFYLYYFIYPAISVLLIIWIFYLLMKDKTK
                     QVFSPQNKKILHAALFLFILSLANGQRGIVRHGFAEMGDLTVNATFFLACSLVLIYIL
                     RVNGKQQIQTSFYGFSFAVFLLLKFFPHQPEKGMFERAITETAFLTLHTDLHTKKPFT
                     RTVVNESISEMTQEFKSFLDANLKPNQTFLDFSNSPILYSYSGRKVPGYFCQNLQNTV
                     DDYLQFHLLKSVDTKTVPVVVFSNYPPTWFDKTDDVPNTMRYYWIAEFIYKNYTPYAI
                     IGGKDIWIANDFRLKQDKEYSHDNRLLISKNYENKHLPRLTYLFHEKEGFEGLEFAET
                     LTLKNDTIAAFNLQKTNFHHHCYVLISYKEMKNNEKKTVSLCDEKGNEYESFSFYGQE
                     NVSNYMLRISSARFWHSGLVTSLKIKSEYGKDMLHKITVIKDNRIEN"
BASE COUNT         1247 a          603 c          658 g         1281 t
ORIGIN      
        1 tacacctact ggaacgatgt ttacaaaggt gaaaaaggcg atttaccatt gagtttttat
       61 gtgcttaaaa ataatttaaa ggttgctcaa acccattttt tggaagttta ccgtatgttg
      121 gaggcctttg aagattggtt gggtccctac cctttttatg aagatggcta taaattagtg
      181 caagtgccct atttaggtat ggaacaccaa agtgctgttt cttatggcaa tggttttacc
      241 aacggttatt tgaattcaga cctatctgaa acaggttggg gaatgaattg ggattttatc
      301 atcattcatg aaagtggtca cgaatggttt ggaaacagca ttactagtag cgatattgct
      361 gatatgtgga tacacgaagc ttttacaacc tatacggaag aaattttcac agaatattat
      421 tggggcccaa cagcgggtga tgaatacata agagggatcc gacatcgaat tttaaatgac
      481 gagcctataa ttggcaaata caacgtgcaa tattcgggta cttcggacat gtatttcaaa
      541 ggagccaatt tattgcatac cattcgccag attgttaaca acgacgcaaa atggaaacaa
      601 ttttttcgtg atttcaactt ggtttttcgt cataaaatta caacgactga ggatgtggag
      661 aaatttatga gtgcttattt caacattgat ttaacttctg tattcaatca gtatttaaga
      721 accacaaacg ttccatcgct gtatttgaaa aagaaaggca aatattgggt gtataaatgg
      781 aacaatgttg ttaacgattt tacaatgcct ttgcacgtga tggttgacga cacactacaa
      841 accatcgttc caacattaaa gcaacaaaag ttgaaagcaa aatgtatcgc cattaacaac
      901 aattactaca taaaagtgaa cggtcgtttc ttttagtaac gctattaaat cgtcttgtta
      961 ttcaatatta tagggcacag ataaaatcca agtaagacaa tatctttttt gtaaaattca
     1021 aagagtgata ataaagtttt tttaactgat tgaggttgtt atttccttac acatttaaag
     1081 tgttcgtttt accaatccca aagaaggtgt gtattctgta tgataaataa tcggactttt
     1141 tttattgcta tcctttgtgt ttgcttctcc attaagtcgt ttttttacag cttcaaacac
     1201 ctcttccaca gtgataagtt tcatacatgt tttttctcca ttacatggtg caatgttgaa
     1261 atcatgtacg caaggactac aaaatacatt tttataaata atttccacat tgctagtcca
     1321 accatattga tctggagagc aaggaccgaa aagacaaatt gaaggagttt cacaagcaaa
     1381 accaatgtgc atcggacctg tatcgttggt aaccattaat gttgcatttt taatcaaata
     1441 gattaattcg tcgatgctcg ttttcccggc aatgttttgg gtaagttgtg aatttgtgtt
     1501 ttcagttacg tattgtgaat attgccattc atttttacta ccgattatca atactttgta
     1561 ttgtggaaaa gtgtcattaa ttaaatgaat taactccttg aatttctttc gtccccacct
     1621 tctctcaata cgtaaatcgg aagcattagg attaattaag atatactttt cctttggaaa
     1681 atcgaaattt gaaacctgaa tatttgtgta aattggaaac aattttggcg caattgctgg
     1741 ttttccgaac aatcgtgcaa tttgcatata aacttctgaa attggtgctt ttacattgaa
     1801 aaacatcatt tgtgtataaa ttcccattcg aaaagagctt gagcgaagat agaagccgat
     1861 tctgtttttt gaaaatgtaa gtaacgtaaa aagcgtactg aaattcgaat aaatttctaa
     1921 atcaatataa acttcagggc gatgacgaat caatttgaat aatgcttgta aaaccgtttt
     1981 tgatagaata aaaacatttt tatcattaat agtaacaatg tgattgatag tctttatttt
     2041 ttgaagtatt gcccgattag attgtgtcga aacaaaggtt atttctgcct caggataacg
     2101 acttcttatc gcttcaatca ttggcgttgc ttgaatgatg cttcccattc ctttgaattt
     2161 acaaattacg atgcgcttga aatcacggtc aagactgtgg tcgatagata acaatttacc
     2221 aaccaacctt accaaaaagt ttagaaggta aacaaaaggc ttgacaatat agttatcaat
     2281 aaatagttga tgtctagttt tcaattcgat tgtcttttat tacggttatt ttatgaagca
     2341 tatcttttcc atattccgat tttattttca gtgatgtcac caatcctgaa tgccaaaagc
     2401 gagcagatga aatccgtagc atataattgg atacattttc ctgaccataa aatgagaaag
     2461 attcatattc atttcctttt tcatcacata gtgatacggt tttcttttca ttatttttca
     2521 tttctttata agagataaga acataacaat gatggtgaaa atttgttttt tgtagattaa
     2581 acgcagcaat agtatcgttt tttaaagtta acgtttctgc aaattccaat ccttcaaaac
     2641 cttccttttc atggaataaa tatgttaaac gaggcaaatg tttattttca taatttttag
     2701 agataagaag tctgttgtcg tgcgaatatt ctttatcttg tttgagacga aaatcattgg
     2761 ctatccagat gtctttacca ccaatgattg catatggtgt atagttttta tagataaatt
     2821 ctgcaatcca ataatagcgc atcgtattgg gcacatcatc tgttttatca aaccacgttg
     2881 gtggataatt agaaaataca acaacaggaa cagtctttgt gtcaactgat tttaaaagat
     2941 gaaattgcaa ataatcgtca acagtgtttt gcaggttttg acaaaaatat cctggaactt
     3001 ttctgcctga atagctgtat aaaattggag aatttgaaaa atctaaaaag gtctggttag
     3061 gtttcaaatt tgcatctaaa aagcttttaa actcttgtgt catttctgaa attgactcgt
     3121 tgacaaccgt tcgagtaaaa ggtttttttg tatgtaaatc agtatgaagg gttaaaaaag
     3181 ctgtttctgt aattgccctt tcaaacattc ctttttctgg ctgatgagga aaaaacttta
     3241 gaagtaaaaa taccgcaaaa gaaaaaccat aaaaagaagt ttgaatttgt tgcttgccgt
     3301 ttactcgcaa tatgtatatc aagacaagac tacaagctaa aaagaacgtt gcatttactg
     3361 taagatctcc catctccgca aaaccatgtc taacaattcc tcgctgacca tttgctaaag
     3421 acaagatgaa taaaaataac gctgcgtgca gaattttctt gttttgcgga ctaaaaactt
     3481 gtttggtttt atccttcatc aacaagtaaa aaatccagat aatgagtaat acgcttatgg
     3541 caggatatat aaaatagtaa agataaaact gttgaggata actatttgcg atagaagtat
     3601 atccatgtgc ttgatttgct tttacatagt gcaaagccgc cataaaatta tcgtagatat
     3661 aagagccatt tctcaacagt aatgcaatac ctagcataga aaccaaaagt gcgagcaaaa
     3721 atactacggc ttttgcaatt ttttgatagt ttattttttc attggagaga acgagcatta
     3781 tcggtaaat
//
LOCUS       DAOQVQ010000199         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27354_length_3787_cov_4.656752, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000199 DAOQVQ010000000
VERSION     DAOQVQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27354_length_3787_cov_4.656752"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKN22_06785"
     CDS             complement(<1..239)
                     /locus_tag="PKN22_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42447.1"
                     /translation="MKHFVFLLLSLLLFACSNSQQEKARTHHQTAVKYAKHFRFATQQ
                     GKEVLEIISPDTKEVVQTLQPLTENKKSIAISATI"
     gene            complement(323..769)
                     /gene="rplI"
                     /locus_tag="PKN22_06790"
     CDS             complement(323..769)
                     /gene="rplI"
                     /locus_tag="PKN22_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNS42448.1"
                     /translation="MEVILKKNVDKLGYANEIVTVKPGYGRNFLIPQGYAVLATASAK
                     KAHEEVMRQKAHKESKILAEAQEVAGKLEAVSVSIVTKAGENGKIFGSVNTVQLSEAL
                     KKEGFDIDRKSLKIKEEPIKEVGTFEAVANLHKEVIATFKFSVVAE"
     gene            complement(807..1070)
                     /gene="rpsR"
                     /locus_tag="PKN22_06795"
     CDS             complement(807..1070)
                     /gene="rpsR"
                     /locus_tag="PKN22_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNS42449.1"
                     /translation="MAQNDIRYLTPINIEIRKDKYCRFKKSGIKFIDYKDADFLLKLV
                     NEQGKILPRRITGTSAKYQKRVNKAIKRARHIALLPYVGDMLK"
     gene            complement(1096..1431)
                     /gene="rpsF"
                     /locus_tag="PKN22_06800"
     CDS             complement(1096..1431)
                     /gene="rpsF"
                     /locus_tag="PKN22_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNS42450.1"
                     /translation="MNFYETVFILTPVLSDDQAKEAVQKIENDIKSFGGVIKHSENWG
                     LRKLAYPIQKKSTGFYFLVEFEAESPAIAGIETELKRDERVIRFLTVKMDKDHVAYAE
                     RRRAKLAKA"
     gene            complement(1529..>3787)
                     /gene="uvrA"
                     /locus_tag="PKN22_06805"
     CDS             complement(1529..>3787)
                     /gene="uvrA"
                     /locus_tag="PKN22_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNS42451.1"
                     /translation="VDGEIVDIQKGMKLDRYKIHDIELVIDRLLIDPKDRKRIYDAVV
                     LSMKKGDKQMMVMDFDTNEVRHFSRLLMCPTSGISYPEPEPNLFSFNSPYGACSTCNG
                     LGEVSEASLDKIIPDASKSIKQGGLAPLGEYKNTWIFGQLENYLTAEGFSIKTPLKDI
                     PEEVLNVILYGNSDLERTSKNAVTFDGIMHILDRHSEESSAAIQRWAQSFMNQVVCPT
                     CNGHRLKKEAMYFKIADKNIGELAALDLSELSIYFSDIEHHLSVDQQVIATEILKEIR
                     ARLGFILDVGLEYLTLNRSAKTLSGGEAQRIRLATQIGSELMNVLYILDEPSIGLHQR
                     DNTRLINSLKRLRDTGNSVIVVEHDKEMIESADFIVDIGPKAGKHGGKIVNATTLDKL
                     MESDSLTTQYLKGTKKIEIPTTRRKGNGNFLELKGATGHNLKNVNLKIPLGTITCVTG
                     VSGSGKSSLINQTLYPLLLAEIYNGVRKPLPYKSIHGLEHLDKVIEIDQSPIGRTPRS
                     NPATYTKVFDEIRTLFSSLPEAQIRGYKPGRFSFNVAGGRCETCKGGGMKLIEMNFLP
                     DVYVECETCNGKRYNRETLEVRYKGKSISDVLEMTISEAVVFFESVPKIYRVLDTLQS
                     VGLGYIAIGQSSTTLSGGEAQRIKLAAELSKKGTGKTIYILDEPSTGLHFEDIHNLLD
                     VLQRLVNEGNTVLVIEHNLDIVKVADYIIDVGPEGGKGGGQIIATGTPEEVVKKYSKQ
                     SHTARYLEMELK"
BASE COUNT         1096 a          780 c          648 g         1263 t
ORIGIN      
        1 ataatggtag cagaaatggc aatacttttt ttattctctg ttaatggttg caaggtttgc
       61 acaacttctt tggtgtctgg gctaatgatt tccaacactt ctttaccttg ttgcgtagca
      121 aagcgaaaat gtttggcgta tttaactgcc gtttgatgat gagttcttgc tttttcttgt
      181 tgtgaattgg aacaagcaaa caaaaggagg cttaacaata aaaatacaaa atgcttcata
      241 ggttgtgaag ttataaaatt tggtttaaat gcataaaaaa agtcccgaat aaatcgggac
      301 ttctcaatat tggtgattgc tattattcag ctacaacact aaatttgaaa gtagcgataa
      361 cttctttgtg taagttagcc actgcttcga aagtaccaac ttctttgatt ggttcttctt
      421 taattttcaa tgatttacga tcaatgtcaa atccttcttt tttcaaagct tcagacaatt
      481 gaacagtgtt tacagaacca aagatttttc cattttcacc tgctttagta acgatagaaa
      541 cagatactgc ttccaattta ccagcaactt cttgtgcttc agccaaaatc ttagattctt
      601 tgtgtgcttt ttgacgcatt acttcttcgt gtgctttttt agcagaagca gtagccaaaa
      661 cagcgtaacc ttgaggaatc aaaaagttac gtccatatcc tggttttacg gtaacgattt
      721 cgttagcgta acccaattta tctacatttt tctttaaaat tacttccatt ttcttaatat
      781 tttattgcaa attcaatatg taaggcttat ttcaacatat ctccaacgta tggaagaaga
      841 gcgatatgac gtgctctttt gatagccttg tttactcttt tttggtattt tgctgaagta
      901 ccagtaattc tacgaggtaa aattttacct tgttcgttaa ccaatttcaa caaaaagtca
      961 gcgtctttgt aatcaataaa tttgattccg cttttcttga atctacagta tttgtctttt
     1021 ctaatttcga tgttaatcgg tgttagataa cgaatatcat tttgagccat ttctctaaat
     1081 tttaatgatt aataattaag cttttgccaa tttagctctt cttctttctg cgtaagctac
     1141 atggtctttg tccattttca cagtcaagaa acgaatcaca cgctcatcac gttttaattc
     1201 agtttcaatt ccagcgatag caggactctc agcttcaaat tctactaaga aataaaatcc
     1261 agtagatttt ttttggatag ggtaagccaa tttgcgtaat ccccaattct ccgagtgctt
     1321 aattacacct ccaaatgact tgatgtcatt ctcgattttt tgcactgctt cctttgcctg
     1381 atcgtcagac aaaacgggag ttaaaatgaa aacagtttcg taaaaattca taatttttat
     1441 cttttaaatt tgatttataa gggcgcaaag ataaggattt taattaataa accaaaggaa
     1501 tgacaatatt ttacacaaaa ttaagtcctt actttaattc catctccaaa tagcgcgcag
     1561 tgtgcgattg cttgctgtat tttttcacta cttcttcggg tgtacccgtt gcaatgattt
     1621 gtccacctcc ttttccacct tctggtccca catcaataat gtaatcggct acttttacga
     1681 tgtctaaatt gtgctcaata accaatacgg tattgccttc gtttaccaag cgctgcaaca
     1741 catccaacaa attgtgaatg tcttcaaaat gcaaaccggt tgaaggctcg tctaaaatat
     1801 aaatggtttt tccagttcct tttttagata actcagctgc taatttgatg cgttgtgcct
     1861 ctccaccaga taaagtagtt gatgactgac caatggcaat atatcccaac ccaaccgatt
     1921 gcaaagtgtc tagtactcgg tagattttag gaacggattc aaaaaacaca acggcttctg
     1981 aaattgtcat ttccaacaca tcgctgatgg atttcccttt gtagcgcacc tccaaggttt
     2041 cgcggttgta acgcttgcca ttgcaggttt cacattccac atacacatcg ggcaaaaagt
     2101 tcatttctat gagtttcata ccgccaccct tgcaggtttc gcatcgtcca cccgccacgt
     2161 taaatgaaaa acgaccgggt ttgtatcctc taatttgtgc ttcggggagg gaactaaaca
     2221 aggtgcgaat ttcgtcaaaa actttggtgt aggtagctgg attggaacga ggtgtgcgtc
     2281 caattggcga ttgatcgatc tcaattactt tatccaaatg ctccaacccg tgaatggact
     2341 tgtagggcag aggtttgcga acgccattgt aaatttctgc caacaacaaa ggatacaaag
     2401 tttggttaat caacgatgac ttgccgctgc ccgatacacc cgtaacacac gttatagttc
     2461 ctaacggaat ttttaaattc acatttttga ggttatgtcc tgttgctcct tttaactcta
     2521 agaaattacc attgcctttt ctacgtgttg ttggaatttc aatttttttg gttcctttta
     2581 aatattgggt ggtcaacgag tcggattcca tcaatttatc caaagtggta gcattaacaa
     2641 ttttcccgcc gtgttttcca gctttcggac caatatctac aataaaatcg gcactttcaa
     2701 tcatttcttt gtcgtgctcc accacaatta cggaattccc tgtgtcacgt aaacgtttca
     2761 acgaattgat taatcgtgta ttatctcgtt gatgcaagcc aatggatggt tcgtctaaaa
     2821 tgtacaaaac attcatcaac tcagagccaa tttgggtagc caaacgaatg cgttgtgctt
     2881 cgcctcctga aagtgttttt gctgaacgat tgagggttaa atattccaac cctacatcta
     2941 agataaatcc taaacgtgcc ctaatttctt ttaaaatttc ggttgcaatc acttgttggt
     3001 caaccgataa gtgatgttca atatctgaaa aataaatgct gagttccgac aaatccaaag
     3061 cagccaattc accaatattt ttatcggcaa ttttaaaata catggcttct tttttcaaac
     3121 ggtgtccgtt gcaagtaggg caaaccactt gattcataaa actttgcgcc caacgttgaa
     3181 tcgcagccga gctttcctcc gagtgccgat caaggatgtg cataatacca tcaaaagtaa
     3241 cagcattttt tgaagtacgc tccaaatcag aattgccata caaaataacg tttaacactt
     3301 cttctggaat atcttttaac ggtgttttta tactaaaacc ttcagcagtt aagtaatttt
     3361 caagctgacc aaaaatccat gtgtttttat attctcccag cggcgccaaa cctccctgtt
     3421 tgatggattt ggaagcatct ggaatgattt tatcaagtga tgcttcagaa acttcgccca
     3481 aaccattgca ggtggaacaa gccccataag gcgagttgaa cgaaaacaag ttgggctctg
     3541 gttctgggta actaattcca gaagttggac acattaacaa acgcgaaaaa tgacgcactt
     3601 cattggtgtc aaaatccatc accatcattt gtttgtcgcc ttttttcatc gacaaaacca
     3661 ctgcatcgta aatgcgtttt ctatcttttg gatcaatcaa caaacggtca atcaccaatt
     3721 caatgtcgtg aattttgtaa cggtcgagct tcattccctt ttgaatatca acaatttcac
     3781 catcaac
//
LOCUS       DAOQVQ010000200         3739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27834_length_3739_cov_4.065961, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000200 DAOQVQ010000000
VERSION     DAOQVQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27834_length_3739_cov_4.065961"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKN22_06810"
     CDS             <1..860
                     /locus_tag="PKN22_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNS42452.1"
                     /translation="ITMQPEKSRKIPCLNQDLKQWGYSSGYLFSGDLKYGNIGSYFTD
                     HGFDVVEDENDFPSDLPRGKLNYYDEDLYKQLILRMNKLKEPFLQCGFTGSTHSPYDF
                     PSSKKYQKFDGVEGKFQNSLLYADECLFQFLQNAKKEKWYKNTLFVFVADHGHASNHQ
                     QNPSASAFNRIPCLIYGEPLKQEYRGKRIDKLGSQIDIVRTLLYQMGGDYQKYNWSKD
                     LMNPKAPDFALHAIIRGYGWVRPTGNFTYLMENKVYIENTFPAESFNKELQHCHSLLS
                     LIYEEFKNL"
     gene            867..2690
                     /locus_tag="PKN22_06815"
     CDS             867..2690
                     /locus_tag="PKN22_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNS42453.1"
                     /translation="MTVDQLIELLKLESKAQEERYSVKDKAGFKQLKADGLLLQPIRV
                     ARKSYGFADYPEFEFFLPYPAETNQFKNGSNIQLICGDEQPIAGSLLYLEGNKGEIRL
                     FTSDFPDWLEEKNCGVQLMVDQRTNEIQLKALQALSNSKKMMRLFEYFHQSQPSNTVE
                     SQQPTIAFKNTQLNASQQNAIVHALQTEEVEIIHGPPGTGKTTTLVELIYQAHQQGKK
                     MLVTAPSNTAVDNIGVRLAALGIEFLRVGNNVKVNDAILPYTIQGNIDKSNLQQTIKK
                     MRIQSEQLRKMAHQYKRNFGKDERDQRKLLLNEVKNIRNEIRALQQHFETSLYEKNNI
                     ILGTPIGIYDCDFKPETFDFLIIDEAGQCLEPLTWVVLPLAQRFIFAGDPFQLPPTVI
                     SNEAQRKGFGVSLLETIVNNNHSVHLLDTQYRMEAVIAEFSNRYFYHGKLKSIQQHQP
                     DGQQLFFYDTAGADYTEEEHEDTASLLNLEELYFIKRLVSAPEFDEKEAVFITPYNGQ
                     LQKAKEVLIDTPIKRFSTIDSFQGQEAACIILSLVRSNPSQQIGFLNDYRRINVALTR
                     AQKKLYVVGDSSTIGADDFYSKMLDYFEEIGAYHSVFEIQD"
     gene            complement(2687..3220)
                     /locus_tag="PKN22_06820"
     CDS             complement(2687..3220)
                     /locus_tag="PKN22_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS42454.1"
                     /translation="MNKKLKLWELNRVSEEEFKAQQKFPLIVILDDIRSLNNIGSFFR
                     TADAFNVEKIYLCGITATPPHREIQKIALGATDSIDWEYRNSITELIAELKENDVKVV
                     TIEQTEQTTLLQNIHTLDGNKFALVFGNEVNGVNQTIVNASDYVVEIPQFGTKHSLNV
                     SVCAGVVLWEFAKRFIP"
     gene            complement(3217..>3739)
                     /locus_tag="PKN22_06825"
     CDS             complement(3217..>3739)
                     /locus_tag="PKN22_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNS42455.1"
                     /translation="HGKSLSGTTLESLKRYGVIKEIIAGRQFPAIVIAPQTANGWNPA
                     SIKEVIDSVRKKYKTDERRLYVVGMSMGGYGTINFTGTYPEMVAAAVAMCGGGDTRLA
                     EGLSTVPLWIRHGNKDYVVPISESQKIVNAIKKITDKNLIFTVDNGLNHGNMERFFRG
                     EEIYNWLFEKVKP"
BASE COUNT         1229 a          722 c          669 g         1119 t
ORIGIN      
        1 caattaccat gcaacccgaa aaatcacgta aaattccttg cttaaaccaa gatttaaaac
       61 aatggggata cagctcgggc tatttattca gtggcgattt gaaatacgga aacattggaa
      121 gttattttac cgaccacgga tttgatgtgg tggaagatga aaacgatttt ccgtccgatt
      181 tgcctcgtgg aaaattaaac tattacgatg aagatttgta caaacaactc atcttgcgaa
      241 tgaacaagct aaaagagcca tttttacaat gtggttttac tggaagcacc cattcaccgt
      301 atgattttcc atcatccaaa aaataccaaa aatttgatgg ggtagaagga aaatttcaaa
      361 attcattgct ctatgctgat gaatgcttgt ttcaattttt gcaaaatgct aaaaaagaaa
      421 aatggtataa aaataccttg tttgtttttg ttgccgacca cggacacgct tccaatcacc
      481 aacaaaatcc atcggcaagt gcatttaatc gcataccatg tttaatttat ggagagccct
      541 tgaagcaaga atacagagga aaacgcatcg ataaattggg ttcacaaatt gatattgtgc
      601 gcactttgct ttatcaaatg ggaggcgatt accaaaaata caattggtcg aaagatttaa
      661 tgaatccaaa agcacctgat tttgcacttc atgccatcat tcgtggctat ggttgggtgc
      721 gaccaacagg aaatttcact tatttgatgg aaaataaagt ctatattgaa aacacatttc
      781 cagcagaaag ttttaacaaa gaattacagc attgccactc cttgctttcg ttgatttacg
      841 aagaattcaa aaatttataa ccacgtatga ctgttgacca actaattgag ttgttgaaac
      901 ttgaatccaa agctcaagaa gaacgatatt ctgttaaaga taaagctggc ttcaaacagt
      961 taaaagccga tggattattg ttgcagccca ttcgtgttgc ccgaaaatcg tatggttttg
     1021 ccgattatcc cgagtttgaa tttttcttgc cctatccagc cgaaaccaat caatttaaaa
     1081 atgggagtaa cattcagtta atttgtggcg atgaacaacc aattgcaggt agtttgttgt
     1141 atttggaagg caataaagga gaaattcgcc tgtttacttc cgactttcca gattggttgg
     1201 aagaaaaaaa ttgtggcgtt caattaatgg tggatcaacg caccaacgaa attcagctaa
     1261 aagccttgca agcattaagc aattctaaaa aaatgatgcg tttgtttgaa tattttcatc
     1321 aatcgcaacc gtcaaataca gttgaatcgc aacaacctac cattgcattt aaaaacacac
     1381 aattaaatgc atctcaacaa aatgccattg ttcatgcatt acaaacagag gaagtagaaa
     1441 ttattcatgg accgcccgga acaggtaaaa caaccacgtt ggtagagctc atctatcaag
     1501 cgcatcagca aggaaaaaag atgttggtta cagcgccaag caatacagca gtggataaca
     1561 ttggtgtgcg tttggctgca ttgggcattg aatttttgcg agtgggcaat aatgtaaaag
     1621 taaatgatgc aattttaccc tataccattc aaggaaatat cgacaaaagc aatttgcaac
     1681 aaaccatcaa aaaaatgcgc attcaatccg aacaattgcg caaaatggca catcaataca
     1741 agcgaaattt tggcaaagat gaacgcgatc aacgcaaatt gttgctcaac gaagtaaaaa
     1801 acatacgcaa tgaaattcgt gcactacaac agcattttga aacttcgttg tatgaaaaaa
     1861 acaacatcat tctcggtacg ccaataggta tttacgactg tgattttaaa cccgaaactt
     1921 ttgatttttt aatcatagat gaagcagggc aatgtttaga accattaaca tgggtggtgt
     1981 tgccattagc acaacgtttt atttttgctg gcgatccatt tcaattgccg cctactgtta
     2041 tttccaacga agcacaacgc aaaggttttg gcgtatcgtt gttagaaacc atcgtaaaca
     2101 acaaccattc ggtgcattta ttggacactc aatacagaat ggaggctgtg attgcagaat
     2161 tttccaaccg ttatttttat catggaaaac taaaatcaat ccaacaacac cagcccgatg
     2221 gtcagcaatt gtttttttac gatacagctg gtgctgatta caccgaagaa gaacatgaag
     2281 atacggcatc gttgctcaat ttagaagaat tgtattttat caaaagattg gtttcagcac
     2341 ccgaatttga tgagaaagaa gctgttttta ttacgcccta caacggacaa ttgcaaaaag
     2401 caaaagaagt tttgatagat acaccaataa agcgtttttc taccattgat agttttcaag
     2461 gacaagaagc tgcgtgcatt attttatcgt tggtgcgttc taatccttcg caacaaattg
     2521 gatttttgaa cgattacaga agaatcaacg tggcattgac aagagctcaa aaaaaattat
     2581 acgttgttgg tgattcatct accatcggtg ctgatgattt ttatagcaaa atgttagatt
     2641 atttcgaaga aataggtgct taccacagcg tttttgaaat acaggactaa ggaataaaac
     2701 gtttggcaaa ttcccacaac accacacctg cacaaacaga aacgtttaaa ctgtgcttgg
     2761 tgccaaattg cggaatttca accacataat ccgatgcatt tacaattgtt tgatttaccc
     2821 cattgacctc gtttccaaaa accagtgcaa atttattgcc atccaacgtg tgaatatttt
     2881 gaagtaatgt tgtttgctcc gtttgctcaa tggttaccac tttcacatcg ttttctttta
     2941 attctgcaat caattcagta attgaatttc ggtattccca atcaatagaa tcggtggcgc
     3001 caagtgcaat tttttgaatt tcgcgatgcg gtggagtagc agtaattcca cagaggtaaa
     3061 ttttttcaac attaaaagca tctgccgttc tgaaaaaaga accaatattg tttaaactac
     3121 gaatatcgtc gagaattaca attaacggaa atttttgttg tgctttgaat tcctcttcag
     3181 aaacacgatt taactcccac aactttagtt ttttattcat ggttttactt tttcaaacaa
     3241 ccaattataa atttcttccc ctctgaaaaa acgttccata ttgccatggt tcaagccatt
     3301 atcaactgta aaaattaagt ttttatcagt aatttttttg atagcattta caattttttg
     3361 agattcagaa attggtacta cataatcttt gttaccatga cgaatccaca gtggaacggt
     3421 tgacaatcct tcggccaagc gcgtatcgcc accaccacac atcgccactg ctgcagctac
     3481 catttctggg taagttcccg taaaattaat cgttccatag ccacccatac tcattccaac
     3541 aacatataag cggcgttcgt ctgttttgta tttttttctt accgaatcaa tcacttcttt
     3601 aatcgatgct ggattccaac catttgcagt ttgaggtgca ataacgattg cgggaaattg
     3661 acgacctgct attatttctt taatcacacc atagcgttta agactttcca aagtggttcc
     3721 cgataaactt tttccgtgc
//
LOCUS       DAOQVQ010000201         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27881_length_3736_cov_3.209726, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000201 DAOQVQ010000000
VERSION     DAOQVQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27881_length_3736_cov_3.209726"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..355
                     /locus_tag="PKN22_06830"
     CDS             101..355
                     /locus_tag="PKN22_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNS42456.1"
                     /translation="MLVTSVSDFRKDIKSYIDRVIKNVETLVINRGKDTGVVIMSLDE
                     YNSLMATNHELSSKKNEQRLDAAIEKLKKGQSFENDLIEE"
     gene            356..613
                     /locus_tag="PKN22_06835"
     CDS             356..613
                     /locus_tag="PKN22_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNS42457.1"
                     /translation="MRYIFVDESWEDYLYWQKHDKKKLMRINDLLKDIPRNPFEGIGK
                     PEPLKHKYAGFWSRRIDEEHRLIYRFEEASILIAKCRFHYD"
     gene            complement(627..1379)
                     /locus_tag="PKN22_06840"
     CDS             complement(627..1379)
                     /locus_tag="PKN22_06840"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNS42458.1"
                     /translation="MALVHSFETSGNKLFRYRGQIPVILFILAIPVVYFTDYEFIKDN
                     DILYYTLLITCALISVSGQIIRSIAIGTSNKNTSGRNTKEGQVAEALNTKGIYSTLRH
                     PLYLGNYLMWIGIVGFTFNVYYVIIVSLLFWIYYERIMFAEERFLERKFGEDYINWSM
                     KVPAFIPSCKNKVKSDIPFSFKTTLRREYSGITATILGFLFVDFLRDWFTSGQVQWKT
                     THLIVLIVALGISLVFRTLKHNTTLLNEDDRS"
     gene            1440..1829
                     /locus_tag="PKN22_06845"
     CDS             1440..1829
                     /locus_tag="PKN22_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNS42459.1"
                     /translation="MKGKTNFELEYVLKTSPKVLNNVLFTPDGLSDWFCSDVNIKDDI
                     YAFDWDGYIEEARLISAKPGQYIKWQWIEDEEDGHDTYFGFSYSTDPITKAVILKVSG
                     SADIEEEEEAKALWEQHVNDLRRLIGA"
     gene            1944..3335
                     /gene="lpdA"
                     /locus_tag="PKN22_06850"
     CDS             1944..3335
                     /gene="lpdA"
                     /locus_tag="PKN22_06850"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNS42460.1"
                     /translation="MNYDIIVIGSGPGGYVTAIRASQLGLKTAVVERDNLGGICLNWG
                     CIPTKALIKSANVFDYLNHASEYGINVKDASIDFNAIIERSRGVASGMSAGIQFLMKK
                     NKIDVIKGTGVVKPGKKVLVTGEDGKQTEYTADKGIIIATGARSRELPNLPQDGEKVI
                     GYRQAMTLKSQPKKLVVVGSGAIGVEFAYFYNAIGTEVTIVEYLPNIVPIEDEEVSKQ
                     LEKSFKKAGVKIMTNASVEKVDTSGKGCVVTVKTDKGEEKIEADVVLSAVGIQANIEN
                     IGLEEVGIATDRGRILVNDFYETNIPGYFAIGDVIPTAALAHVASAEGIICVEKIAGH
                     HPEALNYGNIPGCTYCTPEVASVGMTEKAAKEAGYDIKVGKFPFSASGKASAAGAKDG
                     FVKLIFDAKYGELLGGHMIGANVTEMIAEIVAIRKLEITGEELIKTVHPHPTMSEAVM
                     EAAAAAYGEVIHL"
     gene            3357..3710
                     /locus_tag="PKN22_06855"
     CDS             3357..3710
                     /locus_tag="PKN22_06855"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNS42461.1"
                     /translation="MPFLVERGTFFVPTNYFQFFCIFDSIEFLELLNMSTIQREDIHL
                     IARNSDIPTDETQQLLKKHVYTNAHDWKKFLQLFSISAGTGFVVFGIVFFFAYNWADL
                     HKFVKLGLIVALLLH"
BASE COUNT         1279 a          613 c          718 g         1126 t
ORIGIN      
        1 gcacacattg tacaagggat ttttttgtta tttgaagttt taaaaaattg tgttttaagt
       61 tttttgtaca tatatttgta cataattaaa tacaaatcca atgttagtca caagtgtttc
      121 agatttcaga aaggatataa aatcatacat cgacagggtt ataaaaaacg tagagacctt
      181 ggttatcaac cgtggaaaag acacaggggt tgttatcatg tcattggatg aatacaattc
      241 gttaatggca accaatcacg agttatcttc taaaaagaac gagcaacgat tagatgctgc
      301 cattgaaaaa ttaaaaaagg gacaatcttt tgagaacgat ttaattgaag aataaatgcg
      361 ctatatattc gttgatgaat cgtgggaaga ttatctgtat tggcaaaaac acgacaagaa
      421 aaaattaatg cgtatcaatg atttattaaa agacattcct agaaatccct ttgaaggcat
      481 tggcaaaccg gagcctttaa aacacaaata tgcaggtttt tggtcaagaa gaatagacga
      541 agaacaccga ttaatttatc gatttgaaga agcctctatt ttaattgcaa aatgccgctt
      601 tcattatgat taattcaatc agccacttac gagcgatcgt cttcgtttaa caaagttgta
      661 ttgtgtttca aggttctaaa taccaacgaa atacccaaag caacaatcaa cacaattaaa
      721 tgcgttgttt tccattgcac ttgtccagat gtaaaccaat cgcgtaaaaa gtcaacaaac
      781 aagaatccca aaatcgttgc tgtaataccc gaatattctc ttcttaaggt tgttttgaac
      841 gaaaaaggaa tatctgattt tactttgttt ttgcaacttg gaataaacgc tggtactttc
      901 atcgaccaat tgatgtaatc ttccccaaat ttacgctcta aaaaacgttc ttcggcaaac
      961 ataatgcgct cgtaataaat ccaaaataac aacgaaacga tgatgacata atacacgtta
     1021 aacgtaaagc caacaattcc aatccacatt aaatagtttc ctaaatacaa cggatggcgc
     1081 aacgttgaat aaattccttt ggtgttcaaa gcctctgcca cttgcccctc tttggtattt
     1141 cttcccgaag tatttttatt ggatgttccg atagctatcg atctaataat ctgaccacta
     1201 acagaaatta atgcacaagt tattaacaac gtatagtaca agatgtcgtt atctttaata
     1261 aactcataat cagtaaaata aactactgga attgctaaaa taaataaaat tacagggatt
     1321 tgtcctctgt atctgaacag cttgttgccg cttgtctcaa aagaatgtac taatgccatt
     1381 tgaaaaaata tgtatattgc aaaaaaattg ctacgaaggt ataataaatt acgacaacta
     1441 tgaaaggaaa aactaatttt gagttagaat atgtattgaa aacatcgcca aaggtgctta
     1501 acaacgtgct ttttaccccc gatggacttt ctgattggtt ctgcagtgat gttaacatca
     1561 aagacgatat ttatgctttt gattgggatg gctatattga agaagcacgc ttaattagtg
     1621 ccaaacctgg acaatacatc aaatggcaat ggattgaaga tgaagaagat ggacacgata
     1681 cttattttgg tttcagctat tctaccgacc caataactaa agcggtgatt ttaaaagtaa
     1741 gtggttctgc cgacatcgaa gaagaagaag aagcaaaagc attgtgggag caacatgtta
     1801 acgatttaag acgattaatt ggcgcataac acctcgtttt ttttaacatt attcacttga
     1861 ttcacttttt tattctacaa atggttagaa tacgcaatta tttcactaca ttcgcaatgc
     1921 aaaattttta acagtataaa aatatgaatt acgatattat tgtaataggt tcaggacctg
     1981 gaggatatgt tacagcaatt agagcttctc aattgggctt aaaaactgct gttgttgaac
     2041 gtgacaactt aggaggaatt tgcttaaact ggggatgtat tcctacaaaa gcattgatca
     2101 aaagtgcgaa tgtttttgat tacttgaacc atgcatctga atatggaatc aatgtaaaag
     2161 atgcttctat cgattttaat gcaatcatcg aaagaagtcg cggagttgct agcggaatga
     2221 gtgctggtat ccaattctta atgaaaaaga acaaaattga tgttatcaaa ggaactggtg
     2281 tggtaaaacc tggtaaaaaa gtgttggtaa ctggtgaaga cggaaaacaa acagaatata
     2341 cagccgataa aggaatcatc attgcaacag gtgcacgttc tcgtgaatta cctaacttgc
     2401 cacaagatgg tgaaaaagtg attggttacc gccaagcaat gaccttgaaa tcacaaccta
     2461 aaaaattagt tgttgttggt tcgggtgcta taggtgttga gtttgcttat ttttacaatg
     2521 caattggaac agaagtaaca attgttgaat acttgccaaa cattgttccg attgaagacg
     2581 aagaagtgtc aaaacaattg gaaaaatcgt ttaaaaaagc aggtgtaaaa atcatgacca
     2641 atgcttctgt tgaaaaagtg gatacttctg gaaaaggatg tgttgttaca gtaaaaacag
     2701 acaaaggaga agagaaaatt gaggcggatg ttgtactttc tgctgttggt attcaagcaa
     2761 acattgaaaa cattggttta gaagaggttg gtatcgctac agatagaggt cgtattttag
     2821 taaacgattt ctacgaaaca aatatcccag gttattttgc aattggtgac gtaattccaa
     2881 ctgctgcatt ggcacacgtt gcttctgctg aaggaattat ttgtgttgag aaaattgctg
     2941 gacatcaccc agaagcgttg aactatggaa atattcctgg atgtacctat tgtacgcctg
     3001 aggttgcttc tgttggtatg acagaaaaag cagctaaaga agcaggttac gatatcaaag
     3061 ttggtaaatt cccattctct gcatctggta aagcaagtgc tgctggtgca aaagatggtt
     3121 ttgtgaaatt aatttttgat gcaaaatacg gtgaattgtt gggcggtcac atgataggtg
     3181 ccaacgttac tgaaatgatt gctgaaattg tggctatccg caaattggaa atcacaggtg
     3241 aagaattgat taaaacagtt caccctcacc caactatgtc tgaagcagtt atggaagctg
     3301 cagcagcggc atacggagaa gtaattcact tgtaagcagc tataaacaat tttaaagtgc
     3361 cttttttagt tgaaagaggc actttttttg ttcctaccaa ttatttccaa ttcttttgta
     3421 tatttgatag tattgaattt ttagaattac tcaacatgag caccattcaa cgagaagaca
     3481 ttcacctcat tgcacgcaac agcgacattc caacggatga aacgcagcaa cttttaaaaa
     3541 aacacgttta taccaatgca cacgattgga aaaagttttt gcaattgttt agcatcagcg
     3601 ctggaactgg ttttgtagtc tttggaattg tattcttttt tgcctacaac tgggccgatt
     3661 tacacaaatt cgtgaagttg ggtttaattg tagcattgtt actccattag agagtataca
     3721 actcccacga tcgagg
//
LOCUS       DAOQVQ010000202         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_27940_length_3731_cov_4.342764, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000202 DAOQVQ010000000
VERSION     DAOQVQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27940_length_3731_cov_4.342764"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="PKN22_06860"
     CDS             <1..1513
                     /locus_tag="PKN22_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS42462.1"
                     /translation="RELGKLKDKPFVFTAEITGDFPPHIYPIDISLQLKVGAQVMFVK
                     NDVSFDKQYYNGKMARVIELNNLEVVVECLEDKKRIEVTKHEWENIRYVTDANTGEIK
                     EEVIGTFVQFPLKLAWAITVHKSQGLTFDKAILDVQDVFAPGQAYVALSRLRSLEGLI
                     MLSPMRITGISTDNSIIQYSDSALSPQQLAAILPKAIKNNLYVQLIKTYDWVEMAAQW
                     ASFEKSCQAHSKSSIKGELTPWIKAQNFSIQNVLDPARKFRNQLDALFLAPNYSPEKV
                     LERVQASVNYFFPIFDTVYQSLIKKIIEIGRVKKNKALLEELQELEEALLETILNLKK
                     TAIVVEHIAFGKKIDKETCFGEEIKNYKIARIAKVKHSLINANELFMATNQDDQHDSL
                     FFNSSTSKKKNAPKEKKKHTSEITLEYFESGLSVSDIALKRQLTTNTIYGHLADLVKN
                     EKIAIEQLLDEETLEFLNRTFGKQSLTLTEMKEMVNDDVTYDVLRLYRSHLLR"
     gene            complement(1510..2361)
                     /locus_tag="PKN22_06865"
     CDS             complement(1510..2361)
                     /locus_tag="PKN22_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNS42463.1"
                     /translation="MGILSKYYNIFGLPETASTVEIKRRYRQLVLKYHPDKMGGNQQK
                     FLEIQEAYEYLTGERKVAASTPTSTSRSTSSARNQSVDDRIKRAKQQYKENLYKEYVA
                     KERYFQQLTSGWKWKALSIISKVGAFVAFVLILELFLPYRIESDRIVGSDYRTMSGLT
                     SAGEVRNYYLATGNSIYFEDIPRYLYHTPEILIYKSYIFHNKRKVQFIFSSGKEEFKV
                     QYDWGGHAYLLLPFFLIPLGVVYIQKRTYSYVFFFYLSLYVSSALLLIFFMTQNRIAH
                     LLTIGNY"
     gene            complement(2336..3355)
                     /locus_tag="PKN22_06870"
     CDS             complement(2336..3355)
                     /locus_tag="PKN22_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS42464.1"
                     /translation="MKLTFLTNNEKIKIQVASFLELLTSNSSHFEAKTSGSTGKPKTI
                     SIKKEFAFNSAKATLSFLNIKEQENALLCLSTDTIGGKMMLVRAQFHNLHLIVVEPSA
                     NPLKNISEPIDFMAIAPIQLHSILEENPEKLKSIRSIIVGGGMISDSTIELLKQHQIT
                     VYQTFGMTETISHIALRKVGFENEEYYSTLENIHVYEENNQLVIKAPMLGIDALTTND
                     LVELKNEHQFKWLGRADFVINSGGVKIQIEALENELHQKIKNHFFITSGKDERLGQKV
                     ILVVEGAPVRELEQKSFYSFLKNKYHVPKEIVFVEHFEYSKSDKINRIATLIAVKNGD
                     FKQVL"
     gene            3416..>3731
                     /locus_tag="PKN22_06875"
     CDS             3416..>3731
                     /locus_tag="PKN22_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNS42465.1"
                     /translation="MKKLLGLSMIIGAIALSFSFTNTKYNEKPIPVVVNQEEVGIKFH
                     QLTFDEALKLSKKTGKPIFIDCYTVWCGPCKMLSKNTFTDAKVGEFFNKNFINIKVEM
                     EKD"
BASE COUNT         1293 a          625 c          624 g         1189 t
ORIGIN      
        1 gcgtgaatta ggaaaattaa aagataaacc gtttgtgttt acggcagaaa ttacgggtga
       61 ttttcctcct catatttatc caatcgatat tagtttacaa ctcaaagttg gcgcacaggt
      121 tatgtttgtg aaaaatgatg tatcgtttga caaacaatat tacaacggaa aaatggcaag
      181 agtaatcgag cttaataatt tggaagttgt tgttgaatgc ctcgaagata aaaaacgaat
      241 agaggtgaca aaacacgaat gggaaaatat ccgctatgtt accgatgcaa atacgggtga
      301 aattaaagaa gaggtaatcg gaacatttgt acaatttcca ttaaaactgg catgggcaat
      361 tacggttcat aaaagtcaag gtttaacatt tgacaaagca attttggatg tgcaagatgt
      421 gtttgcaccc ggacaagcgt atgtagccct atcgcgcttg cgttctcttg aaggtttaat
      481 tatgttgagc ccaatgagaa ttactggaat ttcaaccgat aattctatta ttcaatacag
      541 cgatagtgca ctttctcccc aacaattggc ggctatttta ccaaaagcaa taaagaataa
      601 cctgtatgtt cagctgatta aaacttatga ttgggtagaa atggctgcgc aatgggcaag
      661 ctttgaaaaa agttgtcaag cacattccaa aagttcaata aaaggtgagt tgactccgtg
      721 gattaaagca caaaatttca gcattcaaaa tgtattagac ccagcacgca aattcagaaa
      781 tcaattggat gcattgtttt tagctcccaa ttattctcca gaaaaagtac tggaacgtgt
      841 gcaggcatcg gtcaactatt tctttcccat ttttgataca gtttaccaat cgttgataaa
      901 gaaaatcatt gaaattggta gagtgaaaaa aaataaagca cttttggaag aattgcaaga
      961 attagaagaa gcgttgctgg aaactatttt aaacctcaaa aaaactgcaa ttgttgttga
     1021 acacattgct tttggaaaga aaattgacaa ggaaacgtgc tttggtgaag aaattaaaaa
     1081 ctataaaatt gcacgtattg ccaaggtcaa acattctttg atcaatgcaa acgaattgtt
     1141 tatggcaacc aatcaagacg atcaacacga tagtttattc tttaattcaa gcacttcaaa
     1201 aaagaaaaat gccccaaagg aaaagaaaaa acacacttca gaaataacct tagaatattt
     1261 tgaatcggga ctttctgtta gcgatatagc attaaaacgt cagttaacta caaatacaat
     1321 ttatggacac ttggcagatt tagttaagaa tgaaaaaata gctattgagc aattgttaga
     1381 cgaagaaaca cttgaatttt taaaccgaac ttttggcaag caaagcctga cattaaccga
     1441 aatgaaagaa atggtcaacg atgatgttac ttatgatgta cttcgattat accgttcaca
     1501 cttattgcgt tagtaatttc caattgttaa taggtgcgca attcgatttt gtgtcataaa
     1561 gaaaatcaat aataaagctg aagaaacata caaactcagg taaaagaaaa atacatacga
     1621 atacgttctt ttttgaatat aaacaacgcc caacggaatc aagaaaaagg ggagaagtaa
     1681 ataggcatgt ccgccccaat catattgtac tttaaattct tctttgccac tgctaaaaat
     1741 aaattgcacc tttcttttat tgtgaaatat gtaagatttg taaattaaaa tttcaggtgt
     1801 atgatacaga taacgaggaa tgtcttcaaa ataaatggaa ttgcccgttg ctaaataata
     1861 attgcgcact tcgccagcag atgttaaccc cgacatcgtt ctataatccg accctacaat
     1921 tctgtcgctt tcaatacgat agggcaaaaa caattcaaga attaacacaa atgctacaaa
     1981 tgctccaact tttgagatga tgcttaaagc tttccatttc catcccgaag ttaattgttg
     2041 aaaataccgc tcttttgcaa catattcttt gtacaaattt tctttatatt gttgcttagc
     2101 acgttttata cggtcatcaa ccgattgatt tctggcgcta ctagtacttc tactggtaga
     2161 tgttggtgta gaagcagcaa cctttctttc accggttaaa tactcatacg cttcttgaat
     2221 ttccaaaaac ttttgctgat taccacccat tttatcggga tggtatttta aaaccaattg
     2281 ccgatagcgt cgtttaatct caaccgtcga tgctgtttcg ggtaaaccaa agatgttata
     2341 atacttgctt aaaatcccca tttttaacag caattaaagt tgcaattcgg tttattttat
     2401 cactttttga atattcaaaa tgctccacaa aaacaatttc tttaggaaca tggtatttat
     2461 tttttaaaaa cgaataaaaa cttttttgtt ccaattcacg aacaggggcg ccttccacca
     2521 caagtatcac tttttgtccc aatcgttcat cctttccaga tgtaataaaa aagtgatttt
     2581 ttattttttg atgcaattcg ttttccaacg cttcaatttg aatttttaca ccaccactat
     2641 tgattacaaa atcggctctt ccaagccatt tgaattggtg ctcgtttttc aattcaacca
     2701 aatcgttggt tgttaacgca tcaatcccca acatcggcgc tttgataact agttgattat
     2761 tttcttcgta aacgtggatg ttttctaaag ttgaataata ttcttcgttt tcaaagccaa
     2821 cctttcgcaa agcaatgtga gaaatagttt cagtcatccc aaatgtttga taaacagtaa
     2881 tttgatgctg cttaagtaat tcaatagttg aatcagaaat cattccacct ccaacaatta
     2941 ttgagcgaat ggattttaat ttttccggat tttcttctaa aattgaatgc aactgaatag
     3001 gggcaatggc cataaaatca attggttccg aaatattttt aagcggattt gcacttggtt
     3061 caacaactat taaatgcaag ttgtgaaatt gtgcacgaac caacatcatt ttcccaccga
     3121 tggtatctgt acttaaacac aaaagcgcat tttcttgctc tttgatattg agaaaactca
     3181 acgtagcttt tgccgaatta aaagcaaatt ctttttttat cgaaattgtt tttggtttgc
     3241 cagtagaacc tgatgtttta gcttcaaaat ggctagagtt gcttgttaat aattctaaaa
     3301 aagaggcaac ttgtattttt attttttcgt tatttgtgag aaaagttaat ttcatttatg
     3361 atatttggaa caatatttga atgattcaag caaatttatt taaaaaattg aaaccatgaa
     3421 aaagttatta ggattatcaa tgattattgg cgcaattgca ctatcatttt cttttacaaa
     3481 tacaaaatac aacgaaaaac caattcctgt ggttgtaaat caagaagaag ttggaattaa
     3541 attccatcaa ttaacctttg atgaagcatt gaaattatcc aaaaaaacag gaaaaccaat
     3601 ttttattgat tgttataccg tttggtgtgg tccatgtaaa atgctttcaa aaaatacatt
     3661 tacagatgca aaagttggtg agtttttcaa taaaaacttc atcaatataa aagtagaaat
     3721 ggaaaaagat g
//
LOCUS       DAOQVQ010000203         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28132_length_3713_cov_4.539092, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000203 DAOQVQ010000000
VERSION     DAOQVQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28132_length_3713_cov_4.539092"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PKN22_06880"
     CDS             complement(<1..1200)
                     /locus_tag="PKN22_06880"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS42466.1"
                     /translation="MKLSVQKVAKKRVVIIGGGFGGLELSQQLSKSNYQVVLIDRNNY
                     YQFQPLLYQVATCGLESSSIVFPFRKIFHGVKNFHFRLSNVDLILPEENKIQTGLGEL
                     EYDYLVLATGAGNNFFGNKNIEKYALPMKSLNESLHLRNVILERFEQALSAPENEQEG
                     YLTFVVAGGGPTGTELAGALAEMKSNILPKDYPELDFSKMKIVLVEGGTRVLNAMHVE
                     SSERAHQYLKDLGVEIMLSTFVDDFDGNTVTFKGGESIATKSLIWSAGIKGNMIDGLC
                     NAEVLPGNRLKVNEFNQVVGYSTIFAIGDIACMASDNLPKGHPQMAQPAIQQGRNLAK
                     NLIAMSNKQPLNAFKYNNLGSMATVGRNKAVVELPKKRFGGFLAWATWMFVHLRSIFG
                     IKNKWIIF"
     gene            complement(1301..3415)
                     /locus_tag="PKN22_06885"
     CDS             complement(1301..3415)
                     /locus_tag="PKN22_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS42467.1"
                     /translation="MKQIFSLFIAAIISTSLFAQDIPFDKSAFKDNKDGLKEALKNIK
                     AGDEFYLQQPFADYKAAMPFYEKAYNFNPNNAELNYKLGVCNIHTNFRTRALPYFEKA
                     YNLNPLVSADVKYYIGQGYQLLQKWDEAIKNYNEFASKNAGTPNSDPELLKSATKKIQ
                     ECKSGKQLEKKTIRVFIDNMGNNINTEYPEYCMIMNADGSEMYFTSRRNTSTGGLTDA
                     INGGYFEDIFHSKSVGESWSKATNVGEPINTKGHDATVALSPDGSKMLVYIDDNGDGN
                     LYESVRKGDVWSKPKKMDDIICSPYHESSAWYSNDGKRLYFVSSRPLPGSKDKMEKDR
                     DIYYVTWNAEKQKWDNLVRLPDNINTPYDEDGLFVHPDGKTIYFSSKGHNSIGGYDIF
                     STELRPDGTFSDPINIGMPVNTPDDDVFFVLSASGRYGYMTSFREDGKGEKDLYKITF
                     LGDEKQPILNSEDILLAGLNQVVVEKPVEPIVSNRRGELAILKGTIYDDKTKQPLFAK
                     IELIDNVKGEKLAEFDSDPVNGRFLVSLPAGKNYGIAVKADGYLFHSENFDIPKGADY
                     QEYNKDVYMKKVEVGQVIVLKNIFYDLAKYSLRPESKTELDRLIKLLNDNPTIKIQIS
                     GHTDSRGSDEMNKELSRNRAKEVVTYLVKAGIPESRLKYEGYGEDMLIHTDAEIAKLK
                     TEKEKEAAHQENRRTEFKIIAK"
     gene            complement(3426..>3713)
                     /locus_tag="PKN22_06890"
     CDS             complement(3426..>3713)
                     /locus_tag="PKN22_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42468.1"
                     /translation="IADFDLTIALKNDYAAAYNNKASVYLKQEKWNDAVKMCDKAIQL
                     NAELGSAYYNRGIAYEMLRNPKQSCQDWSKAAELGIEAANSYYSNSECYKF"
BASE COUNT         1036 a          756 c          632 g         1289 t
ORIGIN      
        1 gaagataatc catttatttt taataccaaa aatagagcgc aagtgcacaa acatccacgt
       61 tgcccaggct aaaaatccgc caaaacgttt tttaggcaat tctactactg ctttgttgcg
      121 cccaacggtt gccatggaac ctaaattatt gtacttaaag gcattcaatg gttgtttgtt
      181 gctcattgca attaggtttt tagccaaatt tcgtccttgt tgaatagcgg gttgtgccat
      241 ttgcggatgt ccttttggta aattatcgga tgccatgcac gcaatatcgc caattgcaaa
      301 aatagtggag taacctacaa cttggttaaa ttcattgact ttaagtcgat tgcctggcaa
      361 aacttctgca ttgcacaaac cgtcaatcat gttgccttta attccagccg accaaatcaa
      421 tgatttcgta gcgatggatt cacctccttt aaaagtaacg gtatttccat caaaatcatc
      481 tacaaatgtt gaaagcatga tttctacacc caaatctttc aaatattggt gggcacgttc
      541 actcgattct acatgcattg cattaagcac gcgtgttcca ccctctacca aaacaatttt
      601 cattttagaa aaatccaatt ctggataatc ttttggaaga atgttgcttt tcatttcggc
      661 taatgctccc gccaattcgg ttccagttgg tccgccacca gccacaacaa acgttaaata
      721 accttcttgt tcgttttcag gagcacttag agcttgctca aaacgttcta aaatcacgtt
      781 gcgcaagtgc aatgattcat tcaaactttt cattggcaag gcatattttt caatgttttt
      841 gttgccaaag aagttgttgc cagcaccggt tgccaaaact aagtagtcgt attccaactc
      901 gcccaaacca gtttgtattt tattttcttc tggtagaatt aaatccacgt ttgaaaggcg
      961 gaaatggaag tttttaacgc catgaaatat ttttctaaac ggaaaaacaa tggacgacga
     1021 ttccaaacca cacgttgcca cttgatagag caatggttga aactggtaat aattgtttct
     1081 gtcgattaat acgacttgat aatttgattt tgataattgt tgagaaagtt ctaatcctcc
     1141 gaaacctcct ccgataataa ctacgcgttt tttcgctact ttttgaacac ttaacttcat
     1201 gctacaatag attattgttg cgcatggttt ggatgcaaaa ttacaaaaaa tcggatgata
     1261 aacacagtta ccatccgatt aatttattat ttaagttcaa ttattttgca ataatcttaa
     1321 actccgtacg acggttttct tgatgcgccg cttctttttc tttttctgtt ttcaattttg
     1381 caatttcagc atcggtgtgg atgagcatat cttctccgta accttcgtat tttaaacggc
     1441 tttcaggaat tcctgctttc actaaatagg taaccacctc tttggctcta ttgcgcgaaa
     1501 gttctttgtt catttcatca gatccacgag aatccgtgtg tccgctgatt tgaattttaa
     1561 tcgttgggtt atcgttcaac aatttaatca aacggtccaa ctctgttttt gattcgggac
     1621 gcaatgaata tttagctaaa tcgtagaaaa tattttttaa gacaatgact tgtcccactt
     1681 caactttctt catgtacaca tctttgttgt attcttgata atctgctcct tttggaatgt
     1741 caaagttttc tgagtgaaac aagtaaccat ctgctttaac ggcaataccg tagttttttc
     1801 cagctggtaa tgataccaag aaacgtccgt taactggatc ggagtcaaat tctgctaatt
     1861 tttcaccttt tacgttgtcg atgagttcaa tttttgcgaa caacggttgt ttggttttat
     1921 catcataaat agttcctttc aaaatagcca attcacctct tctgttggat acaattggtt
     1981 ctacaggttt ttcaaccact acttgattca aaccggccaa taaaatatcc tctgaattca
     2041 aaattggttg tttttcatca cctaagaacg tgattttata caagtctttt tcaccttttc
     2101 catcttcacg aaacgaagtc atgtagccat aacgccccga tgcagaaagt acaaaaaaca
     2161 catcatcatc gggcgtattc actggcatac caatgttgat agggtcgcta aacgttccat
     2221 cgggtctcaa ttcagttgaa aagatatcgt atccaccaat tgaattgtgg cctttggatg
     2281 aaaaataaat ggttttaccg tcagggtgaa caaacaatcc gtcttcatca taaggtgtat
     2341 tgatgttatc tggcaagcgt acaagattgt cccatttttg cttttctgca ttccaagtta
     2401 cgtagtaaat atctctgtct ttttccattt tatctttgct tccaggcaat gggcgactag
     2461 agacaaaata caatcgtttt ccatcgttgc tataccaagc cgatgattcg tggtaaggag
     2521 aacaaatgat gtcgtccatt tttttaggtt tgctccacac atcacctttg cgtacacttt
     2581 cataaaggtt tccatctcca ttatcgtcaa tgtacaccaa cattttagat ccatctggac
     2641 tcaaagccac cgttgcgtcg tgtcctttgg tgttgatagg ttcacccaca tttgttgctt
     2701 ttgaccacga ttcacctacg ctttttgaat gaaaaatatc ttcaaaatat ccaccgttaa
     2761 tggcatccgt tagtccgcca gtactggtgt ttcttctgga agtaaaatac atttccgatc
     2821 cgtcggcatt cataatcata caatattcag gatattcggt attgatgttg tttcccatgt
     2881 tatcgatgaa cacacgaatt gtttttttct ctaactgttt acccgattta cattcttgaa
     2941 ttttcttggt tgccgattta agcaattcag gatcagaatt gggagttcca gcgttctttg
     3001 atgcaaattc attgtaattt ttaatggctt cgtcccactt ttgcaacaat tgataacctt
     3061 gcccgatgta gtattttaca tcagcactca ccaacggatt taagttgtat gctttttcaa
     3121 aataaggcaa tgcacgcgtt ctgaaattgg tgtgaatgtt acaaacgccc aatttatagt
     3181 tcaactcggc attgttgggg ttaaaattgt atgctttttc ataaaagggc attgcagctt
     3241 tataatcggc aaatggttgt tgaaggtaaa attcatcacc tgcctttatg tttttcaacg
     3301 cttcttttaa tccgtcttta ttgtctttaa atgcactttt atcaaatgga atatcttgcg
     3361 caaacaatga agtactaatg attgctgcta taaatagtga aaatatctgt ttcataaata
     3421 gttgattaaa atttataaca ctcgctgttt gaataataag aattggctgc ttctattccc
     3481 aattcagctg cttttgacca atcttgacaa gattgtttag ggttgcgcaa catttcatag
     3541 gctattccac gattatagta ggcacttcct aattctgcat tgagctgaat ggctttatcg
     3601 cacattttca cagcatcgtt ccatttttct tgtttcagat aaacagatgc tttgttgtta
     3661 tacgctgctg cataatcatt ttttagtgct atagttaaat caaaatcggc aat
//
LOCUS       DAOQVQ010000204         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28344_length_3694_cov_3.309975, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000204 DAOQVQ010000000
VERSION     DAOQVQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28344_length_3694_cov_3.309975"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKN22_06895"
     CDS             <1..760
                     /locus_tag="PKN22_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42469.1"
                     /translation="GTDICDVKLFFPGTNYEINGFDILDYNTTTRVITIQIDIWNLVK
                     PELYLSRSFLRNSPALDACDDSNFTSLVDIWVDTVDTCKPMIEFVYSDKTNCTPTCVD
                     TKSPGCAIMLDSCNGMFKVSPQIYDEEGCVVSGNNICDVPYKMIIYYQAGCHSDVCIN
                     GCNNDCFCKELEDIVFKIATARYPAPLCDCKCVQSVMMSMAQETSLFVKNEGRTLRYP
                     GWIMNEAMFGTRVGEIEAAIQLLAVKEKFCNFSD"
     gene            1199..2572
                     /locus_tag="PKN22_06900"
     CDS             1199..2572
                     /locus_tag="PKN22_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS42470.1"
                     /translation="MQRTRTTGIRATLIPTNCSKEDVLKWMNAQRKLTNYASKHLLRL
                     MKESPSIAEIVFSNNSHYLAYEVHHNILLKHTDFRNYRIELGLRTSVNERLAAALAGR
                     FYHGYGQRNGDMAEGKWEYKLPNLPAITRQNKAIHVKDSVLRKFVDGDEIITSTKQTK
                     NEQVLIENVRFELQGLFGKIYEFKVLIPNNQVHLLKFLSNKSNVDCNIFFINGNLTIV
                     FKVEEEYELAYEPVSTLGLDLNKRENVFAAMSDGEIIQLTSDLRDNILLVKSLDEKIH
                     EVKESKLRRNYRIQWKKARKELSKKFEPIMKRIISKVEDNKYLLAIDGVTQAKNGSFG
                     HAELKEELVRICKKKNLPFIIVPSAHTSSDCSYCFEISKEYNQVSRSKDFKHISCNVC
                     EKTYHADINAARNIAHDGEYIFNNSTMDFPKDKFGRALRSKRPPTSVANRQKKLKFVD
                     YNSNDLF"
     gene            complement(2860..>3694)
                     /locus_tag="PKN22_06905"
     CDS             complement(2860..>3694)
                     /locus_tag="PKN22_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42471.1"
                     /translation="DNVAYYDPDYVTPENVKGVIFPLGGRGTGWGTTDAYAVATGIYE
                     VDPVEDLVVCNDILYILGKIETYGIRTSIIGFTSYGQACGVLKYISTAENAEVGTMLP
                     DFSFAINASNALQICYAGGMICSSEKLEMERIYGSFQLTSSVSTVTSQLSIPSVIELC
                     DADLPTEPRFLIRGPGRLLEIANQSYNFSLYFDYTIVADEIISLDLSQSPIKVFSSIS
                     GDITYALLPASTPGSFKLFPGDNRLIAKFVSNSTTSATYATVAFTRSALAAETLCCDC
                     G"
BASE COUNT         1360 a          507 c          595 g         1232 t
ORIGIN      
        1 aggtacagat atttgcgatg taaaattatt ctttcctggt acaaattatg aaataaatgg
       61 atttgatatt cttgattata atacaacaac aagagtaata actattcaaa ttgacatttg
      121 gaatcttgta aaacctgaac tttatctttc cagatcgttc ttgagaaatt ctcctgcttt
      181 agatgcttgc gatgattcta attttacttc tcttgttgat atttgggttg atactgttga
      241 cacatgtaaa ccaatgattg aatttgttta ttcagataaa actaattgta ctcctacctg
      301 tgttgataca aagtctcctg gttgcgcaat aatgttagac tcttgtaatg gaatgtttaa
      361 agtttcacca caaatttatg atgaagaagg ttgtgttgtt agtggtaata atatctgtga
      421 tgttccttat aaaatgatta tttattatca agctggttgc cattcagatg tttgtatcaa
      481 cggttgcaat aacgattgtt tttgtaaaga attagaagat attgttttta aaattgcaac
      541 agcaagatac cctgccccat tatgtgattg taaatgtgtt caaagcgtaa tgatgagtat
      601 ggcacaagaa acttctctct ttgttaaaaa cgaaggtaga acgttgagat atccaggatg
      661 gataatgaat gaagcaatgt ttggcacaag ggttggtgaa attgaagctg caatacaact
      721 tttagctgta aaagaaaagt tttgtaattt tagtgattag aacttgacaa ataaaaatat
      781 tgtgatatta taagaacata aataaatatc taagatgatt ttgtattttt tgttcatttt
      841 cttttaaaaa cttttgattt aaaaacacat ctatttttta gatgtgtttt tattttaaat
      901 tgatttaaat tggtgcttga caaataaaaa tattgtgata taataatttt atcgtttacc
      961 actaaaaagt atggttttgc cgttccgtaa ctgagagtga acgacgtagt aaaaatggca
     1021 aaaatatcgc aactcactaa aagatacgct ttactgaagt acgtattcga gaagttgtcg
     1081 tatgctcata aatcagtaat ttaacccatt ctaatcgatg ggttaaattt ataccatttt
     1141 taagttttac aataagacac aaaattttgc ttgtattgaa aaaagaaaga agataataat
     1201 gcaaagaaca agaaccacag gaattagagc aacattaatt ccaactaact gtagtaaaga
     1261 agatgtttta aaatggatga atgcgcaaag aaaattaacc aattacgcat caaaacattt
     1321 acttcgtttg atgaaagaat ctccatcgat tgctgaaatt gtatttagta ataactctca
     1381 ttatttagct tatgaagttc atcacaatat tctattaaag catactgatt ttagaaatta
     1441 cagaattgaa ttaggtttac gaacctctgt gaatgaacga ttggctgctg cattagcggg
     1501 aagattttat catggatatg gtcaaaggaa tggagatatg gctgaaggta aatgggaata
     1561 taagttgcct aatttaccag caattacacg acaaaataaa gcaattcatg taaaggatag
     1621 tgtattaaga aagtttgttg atggtgatga aattattact tcaacaaaac aaactaaaaa
     1681 tgaacaagta cttattgaaa atgttcgttt tgaattgcaa ggtttatttg gaaaaattta
     1741 tgaatttaaa gtattaattc caaataatca agttcattta ttaaaattct tatcaaataa
     1801 atcaaatgtt gattgtaata ttttctttat taatggaaat ttaactattg tatttaaagt
     1861 tgaagaagaa tatgaattag cgtatgaacc agtatctaca ttaggtttag atttaaacaa
     1921 aagagaaaat gttttcgctg cgatgagcga tggcgaaatt attcaattaa cttcagattt
     1981 aagagataat attctcttag ttaaatcttt agatgaaaaa attcacgaag ttaaagaatc
     2041 aaaattacgt cgcaattata gaattcaatg gaagaaagca agaaaagaat tatctaaaaa
     2101 atttgaacct atcatgaaac ggattatctc taaggttgaa gataataagt atcttttagc
     2161 aattgacggt gtaacacaag ccaaaaatgg ttcgtttggt cacgcagaac ttaaggaaga
     2221 acttgttaga atttgcaaaa agaaaaattt acctttcatt attgttccat cagcacatac
     2281 ttcaagtgat tgctcttatt gttttgaaat aagcaaagaa tataatcaag tttcaagaag
     2341 taaagatttc aaacatattt cttgcaatgt atgcgaaaaa acttaccatg ctgatatcaa
     2401 tgctgcaaga aatattgctc atgatggtga atatattttt aataattcta caatggattt
     2461 tcctaaagat aaatttggaa gagctttaag atccaaaaga ccaccaacat cagttgcaaa
     2521 tagacaaaag aaattaaaat ttgttgacta taattctaat gatttatttt gacaaataga
     2581 aaaagtatga tataatgatt tcatcatctt taaaataaaa ggtacaggca ggctgtaacc
     2641 gattaaagat gccttgtcaa cctgaataaa aaatccctaa tttttaaggg atttttttat
     2701 ttaaaatgaa tagattttca ctctaaattc aacgaaaaaa gtttttaata gttaatatac
     2761 tattgtgcat tttttaatcg aatttagagt gaaattctct ttaaaatgaa tgaaaaaacg
     2821 gtctatttta gcacattttt gaaaagatca agaagaaaat catccacaat cacaacataa
     2881 tgtttcagca gccaatgctg accttgtaaa cgcaacagtt gcataagttg cacttgttgt
     2941 tgaattagaa acaaacttag caattaatcg gttatcacca ggaaataatt taaatgaacc
     3001 tggtgttgat gctggcaata atgcataagt gatatcgcca gaaattgaac taaaaacttt
     3061 aattggagat tgagaaaggt ctaaacttat tatctcatct gcaacaattg tatagtcaaa
     3121 gtataatgag aaattataag attgattggc aatttctaat aatcttcctg gtcctctgat
     3181 taagaatctt ggctctgttg gtaaatcagc atcgcatagt tcaatcactg atggaattga
     3241 taattgactt gttactgttg aaacagaaga agttaattga aaactaccat aaattctttc
     3301 catttctaat ttttcactac tacaaatcat accaccagcg taacaaatct gtaatgcgtt
     3361 tgaagcatta atagcaaatg aaaagtcagg aagcattgtt ccaacttctg cattttctgc
     3421 tgtgctaatg tattttaaca caccgcaagc ttgaccataa gaagtaaaac caataattga
     3481 tgttcttatc ccataggttt caattttacc taaaatatag agaatatcat tgcaaacaac
     3541 taggtcttca acaggatcaa cctcatatat tcctgttgca accgcataag cgtctgttgt
     3601 tccccatcct gtacctctcc cgccaagagg aaagataaca ccttttacgt tttcaggagt
     3661 aacataatca ggatcataat atgcaacgtt atca
//
LOCUS       DAOQVQ010000205         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28458_length_3684_cov_3.692753, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000205 DAOQVQ010000000
VERSION     DAOQVQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28458_length_3684_cov_3.692753"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2299)
                     /gene="secD"
                     /locus_tag="PKN22_06910"
     CDS             complement(<1..2299)
                     /gene="secD"
                     /locus_tag="PKN22_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNS42472.1"
                     /translation="MRNRGFFWFITILLVVVSIYQISFTFVSNSVEKKVEKEVIGELA
                     NYKELAKKTGDTIVIDGILIDFRKSEGEENAKAALISKKLKEMGETSVYPIIGSSFSE
                     AKKRSLAFGLDLVGGMSVTMEINIPEMVKSFAKERDPKFRKVYDAAVAEYTKTGGDFI
                     KIFADKHKQLNGGTKLVSLFSLIDNEELNIKSTDEEVVAFLYAREKASMNGVEEIINR
                     RVNQFGVSQPNIQKDEKNNRLYVELPGVQDEKTVADQLQSTANLQFFEVYTGQEIASS
                     WQNAVTLSTQKESKVEEIDTTTTNDSVSVKNDTAADISLDNLGDLSNTKTKGLGELIT
                     PLSDNPFMAFATIDDRTLVDQILARKDIQQVFPDDLKFMWSADAEKIDKKMGYMLYAI
                     KIPANGKARVGGKDIEKAMADVNERGQRTVNLRMTTDGAEKWALMTGENVGKAVAITM
                     DNIVYSAPIVQNVISQGNTEITGNFSFEEANRFAGLLNGGALPAPSVIKEQVKVGPTV
                     GADNSRSSILSFAISFLVVLAYVIFYYGKAGRVASIVLVANILFIFGTLASFGAVLTL
                     AGIAGIVLSFAMAIDANVLIYERVREELAAGKDQATAISDGFKKSLNPIIDSNVTSML
                     TAIILVVLGSGPIESFGVALIIGLFTQLFAALVITRLIFDIQMKKGKKITFDIKLTEG
                     AFKDTKIDFAGKRKVFYMISGAFALISLVFLFTKGLNPSVEFSGGRTFAVKFEKSLSG
                     NLDLLEKNLRNSFDSPEAAITLKTKS"
     gene            2563..3300
                     /locus_tag="PKN22_06915"
     CDS             2563..3300
                     /locus_tag="PKN22_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNS42473.1"
                     /translation="MKNIGAIVLIFIIIGFVLNLYLSFFTNHGEKVVVPNLIDKNVGQ
                     IAQLMEELGLEYEVSESVYDPSKPEGTILFQDPLPTSKSKLAVKSGRTIRIKVSLKSQ
                     MVEVPNCVDKSQRFAESILKNRGFKYVVEYVPSTESAGAVIKQLYKGKVIDNKTKVSM
                     GATITLVVGKRSADEQFMLPNLVGLTICDAKNRLTPDMNVNLIVNCVGCATSADSCSA
                     VVSSQSPEFIEGANISGGATITIHATK"
     gene            3312..>3684
                     /locus_tag="PKN22_06920"
     CDS             3312..>3684
                     /locus_tag="PKN22_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42474.1"
                     /translation="MKINHIVLSFGLLTTSLAFSQNNGEGVMPITHNVELLNATKSQF
                     QLKSGSSIDSTFIYTVDTLNIPLFDDFSSNKIQQYNAGYTDPGVTSTLYYRLKDMTNV
                     PLHVDSVYTTQQTFRKTYDINT"
BASE COUNT         1190 a          653 c          590 g         1251 t
ORIGIN      
        1 ttgattttgt ttttaatgta attgccgctt caggagaatc gaaagaattt ctaagatttt
       61 tctccaataa atctaaatta ccgctcaatg atttttcaaa tttcacagca aatgtacgac
      121 caccagagaa ctcaacactt ggattcaatc cttttgtgaa taagaacaca agagaaatta
      181 aagcaaatgc accagaaatc atatagaaca ctttacgttt accagcaaag tcaatttttg
      241 tatccttgaa tgcaccttct gttaatttga tgtcaaaagt aattttctta ccttttttca
      301 tttgaatatc aaaaatcaaa cgagtaatta ccaatgcagc aaacaactga gtaaacaaac
      361 cgatgattaa tgcaacccca aatgattcaa ttggaccaga acccaaaaca accaagataa
      421 ttgcagttaa cattgaagta acgtttgagt cgataattgg attcaatgat tttttgaaac
      481 catcactaat tgccgttgct tgatcttttc ctgctgccaa ttcttcacgg acacgctcgt
      541 agatgaggac attcgcatca attgccattg caaatgaaag aacgataccc gcaataccag
      601 ctaatgttaa taccgctcca aaagaagcta gtgttccgaa aataaacaaa atgtttgcaa
      661 ccaatacaat acttgcaaca cgaccagctt tgccataata gaaaattaca taagccaata
      721 ctactaagaa agatattgca aaagataaga tacttgatct tgagttgtct gctccaactg
      781 ttggaccaac ttttacttgc tctttaataa cacttggagc aggtaaagca ccaccattta
      841 ataagcctgc aaatcggttt gcttcttcaa atgagaagtt acctgtgatt tcagtatttc
      901 cttgagagat tacgttttgt acgattggag cactgtaaac gatgttgtcc attgtaattg
      961 ctaccgcttt tccaacgttt tctcctgtca ttaaagccca tttctcagca ccatcagttg
     1021 tcatgcgtaa gttaactgtt ctttgacctc tttcgtttac atctgccatt gctttttcaa
     1081 tgtcttttcc accaacacgt gctttaccgt ttgctggaat tttaattgca taaagcatgt
     1141 atcccatttt cttatcgatt ttttcagcat ctgccgacca catgaatttt aaatcgtctg
     1201 ggaacacttg ttgaatatct ttgcgagcca aaatttggtc aaccaatgtt ctgtcatcaa
     1261 ttgtagcaaa tgccataaat gggttgtcgc tcaatggcgt gattaattca cctaaacctt
     1321 ttgttttggt attgcttaaa tcacctaaat tatccaatga aatatcagct gctgtatcgt
     1381 ttttaactga aacagaatca tttgttgttg tagtatcaat ttcttcaact ttactttctt
     1441 tttgagtaga aagcgttaca gcattttgcc agctagatgc gatttcttgt cctgtataaa
     1501 cttcgaaaaa ctgaaggttt gcagttgatt gtaattggtc tgcaacggtt ttttcgtctt
     1561 gcacacctgg taattcaaca tacaaacggt tgtttttctc gtctttttga atgtttggtt
     1621 gagaaacacc aaattggttc acacgacggt tgatgatttc ttcaacacca ttcattgaag
     1681 ctttttcgcg tgcatataaa aaggctacaa cttcttcgtc tgtagatttg atatttaatt
     1741 cttcattatc aatcaaagag aataacgaaa ctaattttgt gccaccattt agttgtttgt
     1801 gtttgtcagc aaaaatctta atgaaatcgc cacctgtttt tgtatattct gctactgctg
     1861 cgtcatacac ttttctgaat ttaggatctc tttcttttgc aaaagatttc accatttcag
     1921 gaatgttgat ttccatggta acagacatac caccaactaa gtccaaacca aaagccaatg
     1981 agcgtttttt agcttcagaa aaagaagaac caattatagg ataaacagaa gtttctccca
     2041 tttctttcaa tttcttacta attaatgccg cttttgcatt ttcctcaccc tctgattttc
     2101 tgaaatcgat taaaattcca tcaattacga tagtgtcacc agttttttta gccaattctt
     2161 tataattagc caattcacca attacctctt tttctacttt cttttctact gaattagaaa
     2221 caaaagtaaa tgagatttga tagatagaaa ctactactag tagtatcgta atgaaccaaa
     2281 aaaatcctct attacgcatg ttagtttatt tcgttttatt taatttacaa agtcgcaaat
     2341 atagaattta caaatgtgat agcaccatta attcgaaaaa aaatataaac aaattttatt
     2401 tttagttaag aaattcaatt ttttgtggtt ttttgatttt taaaaactaa tgaatgaatt
     2461 aactgtttta aataagaaat tctatctttg ttttttttat aatgaaaaaa ggcaatggga
     2521 ttatcgcaaa agattaaaaa cttcgtgttt acaaagcaat tcttgaaaaa tattggagca
     2581 attgttttaa tattcatcat cattggattc gttctcaatt tatatctaag tttttttact
     2641 aaccatggag aaaaagtagt agttcctaat ttgattgata agaatgtggg gcaaattgct
     2701 cagttaatgg aagaattagg gttggaatac gaagtatcag aatcggtata tgatccatct
     2761 aaaccagaag gtaccattct ttttcaagat ccacttccaa cttcaaaatc aaaattggcg
     2821 gtaaaaagtg gtagaacaat tcgcatcaag gtttctttaa aatcgcaaat ggttgaagtt
     2881 cctaattgtg tggataaatc acaacgtttt gctgaaagta tattgaaaaa tagaggtttt
     2941 aaatatgtag tggaatatgt gccttctaca gagtcggctg gtgcggttat caaacaattg
     3001 tataaaggca aagtaataga caataaaact aaggtttcaa tgggagctac aatcacatta
     3061 gtggttggaa aacgctcggc tgacgaacaa tttatgcttc caaatttggt agggttaaca
     3121 atatgtgatg caaaaaaccg tttaactcct gatatgaatg taaatttaat tgtgaattgt
     3181 gtgggatgtg caacaagtgc tgattcttgt tctgctgttg tttcttctca aagcccagaa
     3241 tttattgaag gagctaatat ttctggagga gcaacaatta ccattcatgc tactaaataa
     3301 tgggaagaac tatgaaaatt aatcacatcg ttttatcgtt tggcttgcta actacatcgc
     3361 ttgcattttc tcaaaataat ggagaaggag taatgccgat aacgcataat gttgaattat
     3421 tgaatgccac aaaatcgcaa tttcaactta aaagtggttc gtctatcgat agcacgttta
     3481 tttatacagt ggatacgtta aatatcccat tatttgatga tttttcttcg aataaaattc
     3541 aacaatacaa tgcaggatac accgatccag gtgtgacgtc aactttgtat tatcgtttaa
     3601 aagatatgac taatgttccg ttgcatgttg attctgttta cacaacgcaa caaacattcc
     3661 gcaaaacgta tgatatcaat acag
//
LOCUS       DAOQVQ010000206         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28535_length_3676_cov_4.075394, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000206 DAOQVQ010000000
VERSION     DAOQVQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28535_length_3676_cov_4.075394"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3464
                     /locus_tag="PKN22_06925"
     CDS             <1..3464
                     /locus_tag="PKN22_06925"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNS42475.1"
                     /translation="RSRMWEAPDMPLLSESEFKAHFPYLSNSTTSQQFKEVESEIARL
                     AFDTEKSTNVKLNTSNWKTGEYKIETTTKDKYGVEVKDVQFITLTDMRATEVPFPTIF
                     NAYSLKSSYEPGETVKIPFASSLNNQLVLVEVFAAGKLISQQHVRLSNEQKVFEFKVE
                     EQHRGGLNIQLSTIRYGKSYHQEFSILVPYSNKDLKVNFETFRNKLVPGEKEEWRLRI
                     TGPKKEKVAAELLLTMYDASLDVFRTQSMFFSPYAQSTYVDSRHYVGMGVSYGQSYHY
                     RTYGSYNPIGVPQLNFYNFYLNNYYYIDGIPVVKEYSVTMSVRGARDDEAYMSEKKEM
                     APPLEESANAEIDADYTGGLASKNVESLTKTATQTGKEIPSLRTNFNETAFFLPQLKT
                     DSKGDVLVTFTAPESLTSWKILGIAHTKDLQFANIENEAVTQKELMVQTNLPRFVRTG
                     DEVVLTSKISNLTEKGISGSAQLQLFDAATMENVSARFELNQSAVNFSSEAKQSTVAS
                     WKIKVPADFMRSLVVRVTAKAANHTDGEEMTLPVLTDKVLVTETMPMTSNGVGTKAFT
                     FEKLFNNSSTTLTHHSVTMEYSSNPAWYVIQALPYMMEYPYECSEQLFTRFYANAIAS
                     NIVRSTPKIKEVFEKWKSSNPDAFLSNLEKNQELKAVLLQETPWVLDAKNETERKKRI
                     ALLFDFNKMDNELANNLQKLVKAQVSNGGFPWFPGMPESRYITQYIVSGMGHLNELGI
                     TSINEYNDVQNMIKNAINYLDGQLVKDYNYWKKEMAKNKNIQYEPTNLQYLYARSFFN
                     SIKMSKSVQEAFDFFRANTEKRWTEYSLNSQAMLALATHRLGNGTLANDIVKSIMERS
                     ITTEEMGMYWKENTSGYYWYQAPIETQSLIIEALTFITKDVKAINNAKIWLLRNKQTS
                     DWKTTTATAHAAYALLLGGNSFAATPENAIIEINGKQIKPEDFGTQAEAGIGYFKTTW
                     TGKEVSKELAKVKVTRKDDGFSWGAMYWQYFESMEKVTSATTNLKLKKQLFVVQQTNA
                     GEVIVPLADGSKVNRGDKIRVRIELNTDRNLEYVHMKDYRAAGFEPLNVLSSYKWQDG
                     LGYYESTKDVATHFFFDYLPKGTYVFEYDLRVFHSGNFTNGFAEIECMYAPEFKSHSN
                     GVRVVVE"
BASE COUNT         1276 a          644 c          752 g         1004 t
ORIGIN      
        1 cacgctctag aatgtgggag gctcccgaca tgcctttgtt gtctgaaagt gaatttaaag
       61 cacattttcc gtatttgagt aattcaacca cttctcaaca attcaaagaa gtggaaagtg
      121 aaattgcacg attagcattt gacaccgaaa aatcaacaaa cgtaaaatta aacacatcca
      181 attggaaaac aggagaatac aaaatagaaa ctacaaccaa agataaatat ggtgtggaag
      241 taaaagatgt gcaatttatc accttaaccg atatgcgcgc tacagaagtt cccttcccta
      301 ctattttcaa tgcttatagt ttaaaatcgt cttatgaacc gggtgaaacg gtgaaaattc
      361 catttgccag ttcgttgaac aatcaattgg tattggtaga agtttttgct gctgggaaac
      421 ttattagtca acagcacgtt cgcctctcaa acgaacaaaa agtatttgaa tttaaggtgg
      481 aagaacagca ccgtggtgga ctcaatattc aattatcgac cattcgctat ggaaaatcgt
      541 atcatcaaga atttagcatt ttggtaccgt attccaacaa agatttgaag gtaaattttg
      601 aaactttccg caataaattg gtacctggcg aaaaagaaga atggcgtttg cgcatcacag
      661 gacctaaaaa agaaaaagta gctgccgaat tgttgcttac tatgtacgac gcttcgttgg
      721 atgtattccg aacacaaagc atgttctttt ctccgtatgc acaaagcacc tatgttgatt
      781 cacgacatta tgttggcatg ggcgtttctt atggtcaatc gtatcattac agaacgtatg
      841 gttcatataa tcctattggt gttccacaat tgaatttcta caatttttat ttgaataatt
      901 attattacat agatggaata ccagtggtga aagaatattc cgttacaatg agcgttagag
      961 gagccagaga tgatgaagca tatatgtcgg agaaaaaaga aatggcacca ccacttgaag
     1021 aaagtgcaaa tgcagaaatt gatgccgact atactggcgg tttagcgagt aaaaatgtag
     1081 aatcattgac taaaactgct acacaaacgg gcaaagaaat tccatcgttg agaaccaatt
     1141 ttaatgaaac ggcgtttttc ttaccacaat taaaaaccga ttccaaaggt gatgtgttgg
     1201 taacatttac agcacctgaa tcattgacca gttggaaaat tttaggaatt gcccacacca
     1261 aagatttgca atttgccaac attgaaaacg aggcagtaac ccaaaaagaa ttgatggtgc
     1321 aaaccaattt acctcgtttt gtaagaactg gcgatgaagt ggtgttgacc tcaaaaataa
     1381 gcaacctgac agaaaaagga ataagcggca gtgctcaatt gcaacttttt gatgctgcaa
     1441 caatggaaaa tgtgagtgct cgttttgagt tgaatcaaag tgctgtcaat ttttcatccg
     1501 aggcaaaaca aagcacggtg gcatcgtgga aaattaaagt tccagccgat tttatgagaa
     1561 gtttggtggt gcgtgttacg gcaaaagctg ccaaccatac cgatggcgaa gaaatgacac
     1621 ttccagtgtt gaccgacaaa gttttagtaa ccgaaacaat gccgatgacc tcaaacggtg
     1681 taggaacaaa agcatttacc tttgaaaaat tgttcaacaa tagttcaaca acattaaccc
     1741 atcattcggt aaccatggaa tattcatcca accctgcgtg gtatgttatt caagctttgc
     1801 cttacatgat ggaatatccg tatgaatgct cggagcaact atttactcgt ttttatgcca
     1861 atgctattgc aagtaacatt gttcgttcta ctccaaaaat taaagaagtg tttgaaaaat
     1921 ggaaatccag caatcccgat gctttcttgt ccaacttgga gaaaaaccaa gagctaaaag
     1981 ctgttttatt gcaagaaaca ccgtgggttt tggatgctaa gaacgaaacg gagcgtaaaa
     2041 aacgaattgc tttgttattt gatttcaata aaatggataa cgaattggcc aacaacttgc
     2101 aaaaattagt aaaagcacaa gttagcaatg gaggttttcc gtggtttccg ggcatgccag
     2161 aaagtcgcta cattactcaa tacattgtaa gcggtatggg gcacctcaac gaattaggca
     2221 tcacttccat caacgaatat aacgatgtgc aaaacatgat taaaaacgcc atcaattatt
     2281 tggatgggca gttggtgaaa gattacaact attggaaaaa ggaaatggcg aaaaacaaaa
     2341 acattcaata cgaaccaacc aatttgcagt atttgtatgc ccgttcgttt ttcaattcca
     2401 ttaaaatgtc taaatcggtg caagaagcct ttgatttttt ccgtgcaaat accgaaaaaa
     2461 gatggacaga atacagcttg aacagtcaag caatgttagc tttagcaacg caccgtttag
     2521 gaaatggcac attggcaaac gacatcgtta aatcaatcat ggagcgttcc atcactaccg
     2581 aagaaatggg aatgtattgg aaagaaaata ccagtggcta ttattggtac caagcgccaa
     2641 ttgaaaccca atcgttgatt attgaagcgt tgactttcat cacaaaagat gtaaaagcaa
     2701 tcaacaatgc aaaaatttgg ttattgcgca ataaacaaac cagtgattgg aaaacaacaa
     2761 ccgccactgc tcatgctgct tatgcgttgt tattgggtgg aaactcgttt gcagcaacac
     2821 cagaaaatgc gataatagaa atcaatggta aacagataaa accagaagat tttggcacgc
     2881 aagcagaggc tggaataggt tatttcaaaa caacttggac aggaaaagaa gtttcaaaag
     2941 aattggcaaa agtaaaagtt acccgcaaag acgatggatt tagttgggga gcaatgtact
     3001 ggcaatattt tgaatcgatg gagaaagtta caagtgctac caccaactta aaattgaaaa
     3061 aacagttgtt tgtggtgcaa caaaccaatg ccggcgaggt gattgttcct ttagccgatg
     3121 gaagcaaggt aaatcgaggc gataaaatca gagtgcgcat tgagttgaac accgatagaa
     3181 atttagaata cgtgcacatg aaagattatc gtgcagcagg ttttgaacca ctcaatgttt
     3241 tatcgagcta caaatggcaa gatggtttgg gctattacga aagcaccaag gacgttgcaa
     3301 cgcatttctt ctttgattat ttaccgaaag gaacttacgt ttttgaatac gatttgcgtg
     3361 tttttcatag tggtaatttt accaatggtt ttgcagagat tgaatgcatg tatgcgcctg
     3421 agtttaaatc tcactccaac ggtgtacgag ttgtggtaga ataagtgtga tgaattatat
     3481 cgggtggagg attaaaatag aaagtggaat tgctgaggca ctttgttagt tcaagaaagt
     3541 agcaaagaag ttaaaagcat taatttattt cactcaccct aactgtttaa gggtaaaaag
     3601 aactattgaa attagaatct gccctatagt aaataaaaat gttctatcat ttctaatgaa
     3661 ataataattt actatc
//
LOCUS       DAOQVQ010000207         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28669_length_3665_cov_4.126316, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000207 DAOQVQ010000000
VERSION     DAOQVQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28669_length_3665_cov_4.126316"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKN22_06930"
     CDS             complement(<1..412)
                     /locus_tag="PKN22_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42476.1"
                     /translation="MKRFIKIIAESGGTKTDWLAKDESGQFITFTTVSYHPANVNEAF
                     IATQKKIWEQYDLSQADLHFYGSGCLQVAKQEEMKQVFRQLGFCNAISVQSDLFAAWD
                     AVNGASEMVAICGTGSVVFKIENNHCFTKNNLLYD"
     gene            503..1345
                     /locus_tag="PKN22_06935"
     CDS             503..1345
                     /locus_tag="PKN22_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42477.1"
                     /translation="MGENKRRKFGDVFLLESADGEKYILKKANYQQVTQRAIQQLKNE
                     GNFSFQHPGLPFVCSTDETSDSFSLLLNHKNGINLTEFWKSVSKKQRLAFTQQFVQKA
                     VELLDFIHQQAIYHCDIKPSNFIIEGTLDDFNVHLIDFGLAINQHQLEERNLIFPLGF
                     AAPELILNKLHLINPTTDFYALGISVYQLWTGSLPLTHPNPSVFTNLQLAHPLPEHDA
                     LPKKLMQWLSKVCHKPQWRTAPNLMDDNEVEQLLLQSFDKRFTSSHELVEALQQIKQK
                     RWFW"
     gene            complement(1349..2482)
                     /locus_tag="PKN22_06940"
     CDS             complement(1349..2482)
                     /locus_tag="PKN22_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNS42478.1"
                     /translation="MKIALADNGLHKRFAPLTLTRPVGDLRIGILTNTERWKLLANAN
                     IYYQTETYLQGKFPATNDWDILVNAALIPSKEIAQAILTLPDNAALYYNDVELARKGN
                     GSDKNEWLGSQPLIISQRWHLYQLNDEALKQDFTLITKGRTSQPISSTNTIIGNPAHI
                     FLEEGATVEACILNTNAGPIYVGKDAEIMEGCIVRGGLAMGEHSALKLGTKVYGAVTL
                     GPHCKVGGEVNNVVFQSYSNKGHDGFLGNALIGEWCNLGADTNTSNLKNNYGNVSTYS
                     YEAGKEVKTDIQFMGVCMGDHSKCGINTMFNTATVVGVSCNVFGGDFPSKFIPSFSWG
                     GQTIVPFKFEKAVEYSNNMMERRGLQLTTDEVAILKHICDNNW"
     gene            complement(2479..>3665)
                     /locus_tag="PKN22_06945"
     CDS             complement(2479..>3665)
                     /locus_tag="PKN22_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNS42479.1"
                     /translation="EERFGYISYKTGASYFMLSRTLGSAIRLFLATEVLHLFLFGPMN
                     VPYWVTAVVTLLLIWIYTFKGGIKTIVITDTFQTFFLISAVIICTIVIAQALNVDFSG
                     LYNKIAATTTNNGHSMAKILFFDDPNSKLFFPKQFFGGMFIAIAMTGLDQDMMQKNLS
                     CKNIKDAQKNMYSFTTILVITNFLFLLLGGALYVYAFEMGVDLPMKGDKIITDYVFPS
                     LALNNFGLVAGTFFLLGITASSYASADSALTALTTGFCIDFLNFKKREEKERQRLKLI
                     VHIGFSVVFLLIILGTRWYIQKNPGTDLIGLILTLAGYTYGPLLGLFFFGILTKRKMK
                     EVLVPVVCILVPLICYILDKNSVQWLNGYRFGYEMLILNGLLTFGGLYLISNKPIKTN
                     EQ"
BASE COUNT         1168 a          746 c          633 g         1118 t
ORIGIN      
        1 tgtcatataa taaattattt tttgtaaaac agtgattgtt ttctatttta aaaactacac
       61 tgcccgttcc acaaattgcc accatttcag aagctccgtt taccgcatcc cacgcagcaa
      121 ataaatccga ctgaacactg atagcattgc aaaaaccaag ttgacgaaat acctgtttca
      181 tttcttcttg cttggcaact tgcaaacatc ccgaaccata aaaatgtaaa tcggcttgcg
      241 ataaatcgta ttgttcccaa attttttttt gtgttgcaat aaatgcctca ttcacattgg
      301 caggatggta cgaaacggtt gtaaaagtta taaactgccc actttcatcc tttgctaacc
      361 aatcggtttt ggtgccacca ctttctgcaa taatttttat gaatcgtttc attggacaac
      421 aaatgcaaat acaactactt gcaaaactaa gaatttatta cttttgattg ctatgaagtt
      481 gtctgtaaaa aattgtgtgc ctttgggaga gaacaaacgt cggaaatttg gcgatgtttt
      541 tttgcttgaa tctgctgatg gcgaaaaata cattctaaaa aaagccaact atcaacaggt
      601 aacgcaacgc gccattcaac aattaaaaaa tgaggggaat ttttcttttc aacatcctgg
      661 attgccattt gtgtgttcaa cagatgaaac ttctgacagt ttttcgttgc tattaaacca
      721 taaaaacggc atcaatttga ccgaattttg gaaaagcgtt tctaaaaaac aacgattagc
      781 atttacccaa caatttgtgc aaaaggcggt tgaacttctt gattttattc atcaacaagc
      841 tatttatcat tgcgacataa aaccttctaa ttttattatt gaaggaactt tggatgactt
      901 taacgtacac cttattgatt ttggattggc catcaatcag caccaattgg aagaacgaaa
      961 tttaattttt ccgttgggat ttgctgctcc agagttgatt ctcaataaat tgcatttaat
     1021 caatccaact accgattttt atgcgttggg aattagtgtt tatcagttgt ggaccggttc
     1081 attgccacta acgcatccca atccaagtgt ttttaccaac ctacaattgg cacatccctt
     1141 gcccgagcac gatgctttgc ctaaaaaatt aatgcaatgg ttgagcaaag tttgccataa
     1201 accacaatgg cgaacagccc ctaatttaat ggatgacaac gaggtggagc aactactctt
     1261 gcaatcgttt gataaaagat ttacctcctc tcacgaattg gttgaagcct tgcaacaaat
     1321 caaacaaaaa cgttggtttt ggtaagaatt accaattgtt atcgcaaata tgctttaaaa
     1381 ttgcaacctc atcggtggtt aactgtaagc ctctacgttc catcatgttg ttggaatatt
     1441 caactgcttt ttcaaattta aacggcacaa ttgtttgtcc gccccatgaa aacgaaggaa
     1501 taaacttgga aggaaaatca cctccaaaaa cattgcacga aacgccaaca accgttgccg
     1561 tattgaacat ggtgttaatg ccacatttgg aatgatcgcc catgcacact cccataaatt
     1621 gaatgtcggt ttttacttct ttgcctgctt cataggaata agtggaaaca ttgccataat
     1681 tgtttttcaa gttggacgta ttggtatcgg cccccaagtt gcaccattca ccaatcaagg
     1741 cattgcccaa aaatccgtcg tgccctttgt tggaatacga ttgaaaaaca acattgttta
     1801 cttctcctcc aactttgcaa tgtggtccga gggttacagc accatacact tttgttccca
     1861 acttcaatgc gctgtgttct cccattgcca aaccaccacg aacaatacat ccttccataa
     1921 tttcggcatc tttgccaaca taaattggtc cggcattggt gttcaaaata caagcttcaa
     1981 cggtggctcc ttcttctaaa aaaatatgtg caggattgcc aataatggta ttggtagaag
     2041 aaattggctg acttgttctt cctttagtta tcaaggtaaa atcttgcttt agcgcctcat
     2101 cattgagttg atacaagtgc caacgctgac taataatcaa cggttgactt cccaaccatt
     2161 catttttatc actaccattt cctttgcgtg ccaattcaac atcgttgtaa tacaaagcag
     2221 cattatccgg caaggtgagt atggcttgtg caatttcttt agacggaatt aacgctgcat
     2281 ttaccaaaat atcccaatca ttggttgcag gaaattttcc ttgcaaataa gtttctgttt
     2341 ggtaatagat gttggcattg gcaagtaact tccaacgttc ggtgttggtt aaaattccaa
     2401 tacgcaaatc gccaactggt cgggttaatg ttagcggtgc aaaacgtttg tgcaatccat
     2461 tatcggctaa agcaattttc attgttcgtt tgtttttatt ggtttattgg aaatcaaata
     2521 caatcctcca aaagtaagca atccattcaa aataagcatt tcgtagccaa aacggtaacc
     2581 attgagccat tgaacagaat ttttatctaa gatatagcaa attagaggta caagaatgca
     2641 aaccacaggc accaacactt ctttcatctt tcttttggtt aatatcccaa agaaaaatag
     2701 tcccaacagt ggaccataag tgtatcctgc taaggttaaa atcaatccta ttaaatcagt
     2761 tccaggattt ttttgaatgt accaacgtgt tcctaaaata atgagcaaaa acactacaga
     2821 aaatccaatg tgcacaatta atttcaaacg ttgacgttct ttttcttcgc gttttttgaa
     2881 gttaagaaaa tcgatacaga aacccgttgt taatgccgtt agtgcactat ctgctgatgc
     2941 atacgatgaa gcggtaattc cgagtaagaa aaaagttccg gccaccagcc caaaattgtt
     3001 caacgcaagc gaaggaaaca catagtcggt gatgatttta tcgcctttca ttggtaaatc
     3061 cacccccatt tcaaacgcat aaacatacaa agcaccaccc aacaataaaa acaagaaatt
     3121 ggttattact aaaatagttg taaacgaata catatttttt tgagcatcct tgatgttttt
     3181 gcacgataga tttttttgca tcatatcttg atcaagtccc gtcattgcaa ttgcgatgaa
     3241 cattcctcca aagaattgtt taggaaagaa aagtttagag ttgggatcgt caaaaaataa
     3301 aatcttagcc attgaatgtc cgttgttggt ggttgtagct gcaattttat tgtacaaacc
     3361 tgaaaaatca acatttaaag cttgtgcaat gacgatggtg caaataatta ccgcactgat
     3421 taagaaaaaa gtttgaaaag tatcagtaat tacaattgtt ttaatgcctc ctttaaacgt
     3481 ataaatccaa atgagtaaga gcgtaacaac ggccgttacc cagtagggaa cattcatcgg
     3541 gccaaataaa aacaaatgca gcacttcggt agccaaaaac aagcgaatgg cagagcccaa
     3601 ggtgcgcgat aacatgaaat aacttgctcc ggttttatag ctgatatagc caaaacgttc
     3661 ttcca
//
LOCUS       DAOQVQ010000208         3663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28691_length_3663_cov_3.913803, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000208 DAOQVQ010000000
VERSION     DAOQVQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28691_length_3663_cov_3.913803"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PKN22_06950"
     CDS             complement(<1..1539)
                     /locus_tag="PKN22_06950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNS42480.1"
                     /translation="MQQFLNLLFLLSVLNSFAQENLRYSNYCYPVNIPVALAANFGEL
                     RKNHFHMGIDIKTQGKEGFDLLAVEDGFVSRVKVSTYGYGKVVYIDHPNGKTSVYAHC
                     SKFVGRLDSLVKEEQFRTQNFEVELYFTPEQLPVKKGEVFALSGNTGGSTAPHLHFEI
                     RDTKTEHALNPLVYAYDLPDNRKPTIKRVKIFSLNEQGYADYSKTLEKYIKQVNDTLK
                     IVSDTFAIPANFCSSFGGIGFAFDATDKMDGAENTNGIYGSFLVVDGDTVFGHKMNEI
                     SFDNSRYINAHRDLTVAGANYHKSFRNVSNPLEIYTNNGLGVIPIAPGQAKNIVYGAY
                     DEKNNLSKIQFVVVADSGEMGLNFNPSYSNHWYPEDPFEMKRKEWEVVADSFTIYEPY
                     RLMENSDAHICHIGTHLQKKVQVRMRLNNPTKAIEKYYIAVGNKALSTKYNDGWLEAS
                     TNVAGNLTIKTDEQLPVIKPVNASSIVKSSVLKFYISDAHSGLADYDLFIDDKWMLLE
                     YEYKN"
     gene            complement(1549..2514)
                     /locus_tag="PKN22_06955"
     CDS             complement(1549..2514)
                     /locus_tag="PKN22_06955"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNS42481.1"
                     /translation="MKKIGVICGGFSSEYEISLKSGNTIISNFPTNCQAIKIVLSKTS
                     CEASWNNETYPIQLHQKGFQANDEWVELDAYLIYTHGNPGENGKLQGMFDMYNIPYIN
                     SGALASELSFDKWYCNQFLRNFGINVATSQLLLKGDVVNPTTIVEQLGLPLFVKPCDS
                     GSSYGISRVNKVEDLQKAIDVAFNEGDSVVIESFLDGTEVTCGVYRSKDGVQALPLAE
                     IVSENEFFDYEAKYLGKSQEIVPARISEEATLKVQEVAKRVYGILRLRSIIRIDFMLV
                     NNEPYVIEVNTTPGFTEESIVPRMLRCKEVTLRDFWTSIIDFEMS"
     gene            2735..3598
                     /locus_tag="PKN22_06960"
     CDS             2735..3598
                     /locus_tag="PKN22_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42482.1"
                     /translation="MRKIYLLVASVFVGASVFGQTNLSYEDAVNHPYAAAGIPEIGTT
                     NGWALGLYDASTDFSNGAQSVKLTTVTNANFAMAGLPTQLPGQIFQDIDIDATAISNN
                     GADITGTVDVKTNLLGNAAGLDSALIIFEAYDTLAAGSADDVLLYSGSLIITQNTSTW
                     TTVPLSMEDAGNTGTINSLVIGGMSGYKRLTDGSTLMLDNWTIDLGYLSVNNTSNVTF
                     SAYPNPTKNVLNITASEEVSSVSILSLDGKVVTTSTSNTVDVSALTNGVYMYEVTTVN
                     GKKAINKFVKE"
BASE COUNT         1168 a          710 c          575 g         1210 t
ORIGIN      
        1 atttttatat tcatattcca acaacatcca cttgtcgtct ataaacaaat cataatcggc
       61 caagccagaa tgtgcatccg aaatataaaa cttgagaacc gatgatttaa caatcgaact
      121 tgcatttaca ggttttatta cgggcaattg ctcatctgtt tttatagtta aatttccagc
      181 aacattggta gatgcctcca accatccatc gttgtatttg gtagaaagcg ctttattccc
      241 aaccgcaatg tagtattttt caattgcttt tgttggattg tttaatcgca ttcttacttg
      301 tacttttttc tgtaaatggg ttcctatatg acaaatatga gcatctgaat tttccattaa
      361 acggtagggt tcataaatag taaacgaatc tgcaactacc tcccactctt ttcttttcat
      421 ttcaaatgga tcttctggat accaatgatt actgtaggat ggattgaaat tcaatcccat
      481 ttctccagaa tcggcaacca caacaaattg aattttcgat aaattgtttt tttcatcgta
      541 agcaccataa acaatgtttt ttgcctgtcc aggtgcaatc ggaatcactc ccaatccatt
      601 attagtgtaa atttccaacg gattgctaac atttctaaac gatttatggt aattcgcgcc
      661 agcaaccgtt aaatcgcgat gcgcattgat gtaacgcgaa ttatcaaacg aaatttcatt
      721 cattttatgc ccaaaaacag tatcgccatc caccactaaa aaggaaccat aaattccatt
      781 ggtattttct gcaccgtcca ttttatctgt tgcatcaaag gcaaaaccaa tacctccaaa
      841 cgaggaacaa aagttggcgg gaattgcaaa agtatcactt acaattttaa gggtatcatt
      901 gacctgttta atatactttt ccaacgtttt tgagtaatct gcatagcctt gttcgtttaa
      961 actaaaaatt tttactcgtt ttatagttgg ttttctatta tcaggcaaat cgtaggcata
     1021 cacaagcgga ttgagtgcat gctctgtttt ggtatctcta atttcaaaat gcaaatgagg
     1081 agccgtagaa ccacccgtgt tgcccgacaa agcaaacact tctccttttt taaccggaag
     1141 ttgctcggga gtgaaataaa gttccacttc aaaattttga gtgcgaaatt gttcttcttt
     1201 caccaatgaa tcaaggcgac caacaaattt gctacaatgc gcatatacgc tggtttttcc
     1261 attgggatga tcaatataca ccactttccc atagccatag gtagaaactt ttacgcgcga
     1321 cacaaaacca tcttcaacag caagtaaatc aaaaccttct tttccctgtg tcttgatatc
     1381 aatccccatg tggaaatgat ttttacgcaa ttcaccaaaa tttgcagcaa gcgctactgg
     1441 aatatttacc ggatagcaat agttggaata acgtaaattt tcttgagcaa agctattcaa
     1501 tacacttaat agaaacagaa gattaagaaa ttgttgcatt ggttactatt agctcatttc
     1561 aaaatcaata atagaagtcc aaaaatcgcg caaagttact tccttacaac gcaacatgcg
     1621 aggaacaatg ctttcttcgg taaaaccagg tgttgtattt acctcaatta cataaggttc
     1681 gttgttcacc aacataaaat cgatgcggat aattgaacgc aaacgcaaaa tgccatacac
     1741 ccgttttgct acttcttgca cttttagggt tgcttcttcg gaaatgcgtg ccggcacaat
     1801 ttcttgtgat ttacccaagt atttagcttc gtaatcaaaa aattcatttt cacttacaat
     1861 ttctgccaat ggcaacgctt gaacaccgtc ttttgaacga taaacgccac atgttacctc
     1921 tgtgccatcc aagaacgatt caatcaccac gctatctccc tcgttaaacg caacgtcaat
     1981 ggctttttgc aagtcttcca ctttatttac acgagaaatt ccataactag aacccgaatc
     2041 gcatggtttt acaaacaacg gcaaaccaag ttgttcaaca attgttgttg gatttaccac
     2101 atctcctttt aacaacaatt gcgaagtcgc cacgttgata ccaaaattgc gtaaaaactg
     2161 attgcaatac catttatcaa aagacaattc agaagctaac gcacccgagt tgatataagg
     2221 aatattgtac atatcaaaca tgccttgcaa ctttccattc tcacctgggt ttccatgtgt
     2281 ataaattaaa taggcatcca actccaccca ttcatcgttt gcttgaaatc ctttttgatg
     2341 caattgaata ggatacgttt cattgttcca agatgcttca caactagttt tgctcaatac
     2401 aatttttata gcttggcaat tggttggaaa attagaaata atggtattac cactttttaa
     2461 tgaaatttca tattcagaag aaaatccacc acagattacg cctatttttt tcatgtcttt
     2521 tgattttatt caaaaataag tcggcaaacc ttaagaacac ctgaagcaac ttaaatttta
     2581 ttcgctattc attgttgaca agtcaaaatt taatttaata tcgagttgaa tttaatcaaa
     2641 aaaaaacttt ttgtgttaat tttcaacaat acatttgatt tattcctttt ttcaattaat
     2701 tttacgcaat gtttaaactt taaaactttt ttatatgaga aaaatttact tattagttgc
     2761 ttctgtattt gtaggagcaa gcgtttttgg acaaactaat ctttcttacg aagatgcagt
     2821 taatcaccct tatgcagcag caggcatacc tgaaattggt acaactaacg gctgggcatt
     2881 aggtttgtat gatgcttcaa ctgatttctc taatggagca caatcagtta aattaacaac
     2941 tgtaactaac gcaaactttg ctatggcagg tttaccaaca caattaccag ggcaaatttt
     3001 tcaagatatt gatatcgatg caacagctat atcaaataat ggtgctgaca ttactggaac
     3061 tgttgatgtt aaaactaatt tattaggtaa tgctgctggt ttagattcag ctttgattat
     3121 ttttgaagca tacgacacat tggctgctgg atctgctgat gacgtattgt tatattctgg
     3181 ttcattaata attacacaaa atacttctac ttggacaact gttcctttat caatggaaga
     3241 cgcaggtaat actggaacaa tcaattcatt ggtaattgga ggtatgtcag gatataaacg
     3301 tttaacagat ggttctacat taatgttgga caattggaca attgatttag gttacctatc
     3361 tgttaataac acaagcaatg ttactttcag cgcatatcca aacccaacta aaaatgtatt
     3421 gaacattaca gcttctgaag aagttagctc agtatctatt ttatctttag atggtaaagt
     3481 agttacaact tctacttcta acacagttga tgtttctgca ttaacaaatg gtgtttatat
     3541 gtatgaagtt actactgtta atggtaaaaa agctattaac aaattcgtga aagaataata
     3601 aataacacga tatttgaaag gctgttcgat tggacagcct ttttttatta tatttgctcc
     3661 ata
//
LOCUS       DAOQVQ010000209         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28765_length_3657_cov_4.003609, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000209 DAOQVQ010000000
VERSION     DAOQVQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28765_length_3657_cov_4.003609"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKN22_06965"
     CDS             <1..245
                     /locus_tag="PKN22_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HNS42483.1"
                     /translation="KAGVIVQDLTPETLLQTTLFERRNERHIPLMQVVDKINTTYGQQ
                     KIRLASQDQKRVWKMKQQQLSPRYTTKLTDIITINV"
     gene            complement(364..510)
                     /locus_tag="PKN22_06970"
     CDS             complement(364..510)
                     /locus_tag="PKN22_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42484.1"
                     /translation="MTKEAKSERRNKIVKGLEKAYEKMLEFKKKNNSEIAVIKKNKIV
                     RIKP"
     gene            complement(494..1081)
                     /locus_tag="PKN22_06975"
     CDS             complement(494..1081)
                     /locus_tag="PKN22_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNS42485.1"
                     /translation="MKEKNLYIIAGCNGAGKTTASFTILPEILDCKEFVNADEIAKGL
                     SPFQPESVSFEAGRIMLSRIDELFKHDEIFAFETTLATKTYKQKILKASSCGYNITLL
                     FFWLKNVDLAKERVEIRVKEGGHNIPKDVIERRYLNGINNLFNIYLNIVNQVFIFDNS
                     EGHHDLIAEKYLDEDIVIYNQKKYNELKSYHDKRS"
     gene            1164..1289
                     /locus_tag="PKN22_06980"
     CDS             1164..1289
                     /locus_tag="PKN22_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42486.1"
                     /translation="MSRIHNTKKRMPVIIAPENEQDWLQGMPLALQNSRIIATAI"
     gene            1408..2109
                     /locus_tag="PKN22_06985"
     CDS             1408..2109
                     /locus_tag="PKN22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNS42487.1"
                     /translation="MTKTNVLIAGFISAIMAACGTAKVAETKTMYVSATTQPCSAGVM
                     EKECLQVKWDKNQASWENFYNEIEGFTYEAGNEYELLVKESKVANPPADASSLKYTLT
                     KIVSKNKVATAKKPYTSSHVFTAQFDGIQFHKCMGLTSLCPEECGHSGNMAVFTVKSY
                     QEFVVNGQGGTEKLETYRVLISDFHKKDLNQPYVAPIKSLKPGDIVTIAVDYVYDTTK
                     STVQTVENILSITKK"
     gene            complement(2179..3231)
                     /locus_tag="PKN22_06990"
     CDS             complement(2179..3231)
                     /locus_tag="PKN22_06990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNS42488.1"
                     /translation="MTTDTAESLKTSPQIAEKIKQLWNLVGNTPMLEISYLYQGKARK
                     IYAKCEHYNLTGSIKDRMALYVMQKAYEQGAIQPSNVIVEATSGNTGIAFSAIGKSLG
                     HDVKIIMPNWMSQERKDIISSLGAEIIEISKEEGGFVGSIELSQKMAVLDENIFLPKQ
                     FENKYNCEAHEATTAKEIWMQLESIGKKPAAFVAGVGTGGTVMGVGSFLKKQDPAIKV
                     HPLEPAESPVLTAGKKVGHHRIQGISDEFIPSIVELDKLDSIVQASDGDAILVAQQLS
                     KKLGLAVGISSGANFIGAVKLQNELGDDAVVVTIFSDSNKKYLSTDLMKNEPVKDGYL
                     APAIELVDFKSIKRCF"
     gene            3396..>3657
                     /locus_tag="PKN22_06995"
     CDS             3396..>3657
                     /locus_tag="PKN22_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS42489.1"
                     /translation="MKKKAVFNWSGGKDSALALQTILNDNQLEVIALLTTINEDTKTS
                     SIHAIPTSLLEKQAACIGIPLYMVPLAKDTKTYEQGMLEAVEH"
BASE COUNT         1213 a          689 c          558 g         1197 t
ORIGIN      
        1 aaaaagctgg tgtaattgtg caggatttaa cacccgaaac actgttacaa accacgttat
       61 ttgaacgtag aaacgagcgc catattccac tcatgcaagt cgttgacaaa atcaacacca
      121 cttatgggca acaaaaaata cggttggctt cgcaagatca aaaaagagtg tggaaaatga
      181 aacaacaaca gctctcaccg cgttatacaa ccaagttaac cgatattatc accataaatg
      241 tatagttatg tgaaaagcgc cttaaatgcc aaaattgaaa ccttgcacga aaaaccgaga
      301 tttaaaaaag gacgaagatt tgaatacttt ttgtactagg actgttgtta gctgctgtac
      361 aatttacggt ttgattcgta caattttatt ctttttaatt acagcaattt cactattatt
      421 ttttttcttg aattcaagca ttttctcata agctttctca agacctttga cgattttatt
      481 tcgtctttca gatttagctt cttttgtcat gataagattt taattcatta tatttttttt
      541 gattataaat cacaatatct tcatcaagat atttttctgc aattaagtca tgatgccctt
      601 cagaattatc aaatataaaa acttgattta caatattcaa ataaatatta aaaagattat
      661 taataccatt caaatatctt ctttcaatta catctttagg aatattgtgt cccccttctt
      721 tcactctaat ttctactctt tctttagcta aatccacatt tttaagccaa aaaaataata
      781 gagtaatgtt atatccgcaa gaactagctt tcagtatttt ttgtttataa gttttagttg
      841 caagtgttgt ttcaaaagcg aaaatctcat catgtttaaa taattcatca attctactta
      901 acataattct tcctgcttca aatgaaacac tttctggttg gaaaggcgat agtcctttag
      961 caatttcatc agcgtttaca aattccttac agtctaaaat ttcaggtaaa atggtaaaag
     1021 atgctgttgt tttcccagcg ccattacaac ctgcaataat gtaaagattt ttttctttca
     1081 taaatacaaa tttatgattt tattcttgaa gcggtaaatt ttattttggt tactaggttg
     1141 ggtgtattgc agccaacgaa cttatgagtc gcattcacaa tacaaaaaaa cgaatgcccg
     1201 tgattattgc tcctgaaaat gaacaagatt ggctccaagg tatgccgtta gctttacaaa
     1261 acagccgaat tattgcaaca gcgatttaag tgcaaatgtt taactttgtg cacgcataac
     1321 aatttggcgt ttaaacaaat taaatgatat agaaagcttt cttctactta tgaatccttg
     1381 ctatgtaaca tttaattaaa aaacaaaatg acaaaaacaa atgtattgat tgcaggattt
     1441 attagcgcaa tcatggccgc atgtggcaca gctaaagtag cagaaactaa aacaatgtat
     1501 gttagcgcaa caactcaacc ttgttcagca ggcgttatgg aaaaggaatg cttgcaagta
     1561 aaatgggaca aaaaccaagc gtcttgggaa aatttctaca acgaaattga aggttttact
     1621 tatgaggctg gtaacgagta tgaattattg gtaaaagaat caaaagttgc caatcctcca
     1681 gcagatgctt ccagtttgaa atacacgttg acaaaaattg tgagcaaaaa caaagttgca
     1741 actgcaaaaa aaccatatac ttcatcgcat gtgtttacag cacaatttga tggcattcaa
     1801 tttcacaaat gcatgggatt aacttcactt tgtccagaag aatgtggaca ctctggaaac
     1861 atggccgttt ttactgtaaa atcctaccaa gagtttgttg ttaacggaca aggaggaaca
     1921 gaaaaattag aaacataccg tgttttgatt agtgatttcc acaaaaagga tttaaaccag
     1981 ccctatgttg caccaattaa aagtttaaaa ccaggcgata tcgttacaat tgctgttgat
     2041 tatgtgtatg acacaactaa atcaaccgtt caaacagtag aaaacatcct cagcattact
     2101 aaaaaataag tggattttta agacacaaaa aaagctgact ttcaacaagt cagctttttt
     2161 aatgcgttgc catttagctt aaaaacatct tttgatactt ttgaaatcta ccaattcaat
     2221 agcaggtgct aaatagccat cttttacagg ttcgtttttc atcaaatccg tgcttaagta
     2281 ttttttattg ctatcgctga aaatagtaac aactacagca tcatcgccca attcattttg
     2341 aagtttaaca gctccaatga aatttgcccc agaagaaata ccaacagcca atcctaattt
     2401 tttagataac tgttgtgcta caagaatggc atctccatcc gatgcttgca caatgctatc
     2461 caatttatcc aattcaacaa tagaaggaat aaactcatcc gaaatacctt gaatgcggtg
     2521 gtggccaact tttttacctg ctgttaatac aggcgactct gctggttcca acggatgaac
     2581 tttaattgct ggatcttgtt tttttaagaa gctacccact cccatcactg ttcctccagt
     2641 tccaacacct gccacaaaag ccgctggttt tttgccgatg ctttctaatt gcatccaaat
     2701 ttctttggct gttgttgcct cgtgtgcctc acagttatat ttattttcaa actgcttcgg
     2761 aagaaaaata ttctcatcca aaacagccat tttttgtgac aattcaatgc ttccaacaaa
     2821 tcccccctct tctttagaaa tttcaatgat ttcggcacct aaacttgaaa taatatcctt
     2881 gcgttcttgg ctcatccaat tgggcataat aatttttaca tcgtgcccca atgattttcc
     2941 aattgcacta aaagcaatcc ccgtgtttcc acttgttgct tcaactataa cattggatgg
     3001 ttgaatagct ccttgctcat aggctttttg cataacataa agcgccattc tatctttgat
     3061 acttcctgtc aagttataat gctcgcattt cgcataaatc tttcttgctt tcccttgata
     3121 caaataagaa atttctaaca ttggagtatt tccaactaaa ttccataatt gtttaatttt
     3181 ttctgcaatt tgtggtgaag ttttcaacga ttcagcagta tctgttgtca tatattttta
     3241 aatttttatg gcgcaaaatt agagcaatta aactgaatta tagaactatt ctaattttaa
     3301 atttaggacg tattcttgta aagctatcca tcttaaaagc agctaaatca ttttgtttta
     3361 tacatttgca agcaatccta tttttagtat acaaaatgaa gaaaaaagcg gtatttaatt
     3421 ggagtggcgg aaaagattct gcattagcat tacaaaccat tttaaacgac aatcagttag
     3481 aggtaattgc cttgttaaca accattaacg aagacacgaa aacatcctcc attcacgcca
     3541 ttccaacttc acttttagaa aaacaagccg cttgtattgg aattccactt tatatggtac
     3601 cactagccaa ggatacaaaa acctatgagc aaggcatgtt ggaagcggtt gaacatt
//
LOCUS       DAOQVQ010000210         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_28817_length_3652_cov_4.086461, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000210 DAOQVQ010000000
VERSION     DAOQVQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28817_length_3652_cov_4.086461"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKN22_07000"
     CDS             <1..663
                     /locus_tag="PKN22_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNS42490.1"
                     /translation="KHYGVTIADLSAHYKDNDFNKQLRKTLDNSYLGVTVTGTAVVAA
                     EGTRYLIDSLFDGILFAVITVSVLMALLFRNWGIVLVSIVPNLIPLILTGGIMGLLNI
                     PLKPSTLLVFNISFGITSDDSIHFLAKYRQELKKNTGDIAKSVIESLKEVGLSMFYTS
                     IILFFGFSVFMLSQFGGTKALGVLVSTTLIFAISINLLLVPSILYDISRLVNKFKRKK
                     AE"
     gene            complement(665..1000)
                     /locus_tag="PKN22_07005"
     CDS             complement(665..1000)
                     /locus_tag="PKN22_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42491.1"
                     /translation="MKNKNLFQLKTFVAIFDVMNIPKYLKNKYIIALGVFGIYTLFLD
                     DVDIFVIVRQNMKLSKIEQQKEDTQTLLKDTKATLEKLHTLNGIERYAREKKLFKKDN
                     EDIFVISYE"
     gene            1049..1564
                     /locus_tag="PKN22_07010"
     CDS             1049..1564
                     /locus_tag="PKN22_07010"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNS42492.1"
                     /translation="MNFLKLSFILVLVQLFVGCNPSDERKNKLVISKMPHQELSINWL
                     EGLWQCDDKEGVLYEDWKKLNDTLFLGKSYYLADDDTLFVENIQLTLVGDSLSYSSLK
                     RDTLQKNKSFKGVLSTNNGFRVENLQHGFPKSILYNLLNDTLMMIEISGEIDGLVETQ
                     PYQMVKINSKR"
     gene            1564..2124
                     /locus_tag="PKN22_07015"
     CDS             1564..2124
                     /locus_tag="PKN22_07015"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNS42493.1"
                     /translation="MKLDVAPIVEQLKNGATMLYPTDTIWGLGCDATNETACQRVLAL
                     KNRPENKSFIVLVDSFLMLERYIPDFNPICYDLVDLATRPLTIIYPNAKGLAPSVLAE
                     DGSVGIRITKDPICLQLIRGLRKPIVSTSANLSGEKSPTSFATISPQIKSGVDLIVEQ
                     RLNEKMDKASQIIKIELNGTVHVIRK"
     gene            2187..3344
                     /locus_tag="PKN22_07020"
     CDS             2187..3344
                     /locus_tag="PKN22_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNS42494.1"
                     /translation="MQATIEQINACVLIPTYNNEKTLRRVIDGVMQHVSPKHIIVVND
                     GATDATSTILSDYQNSITVLNNEKNRGKGYSLRKGFKHANEKGYSYAITIDSDGQHSP
                     DDLPKIIQACLEHPGAVIMGSRNMQQEGVPGKSSFGNKFSNFWFKVETLISLPDTQTG
                     FRAYPLPPLKKMRLFTTKFELEIEVIVRLAWKGVKFIPVPISVKYDPTERVSHFRPGK
                     DFFRISVLNSVLFIGAMLYFFPKKLLSKGTWEAIKHEAIKPEESNMRKAVSLGFGVFM
                     GIFPIWGFQLLVGIPLAILMRLNKILFIAAANISIPPMIPFIIYASFLVGQYFTADAI
                     DPAQFFHFDLDTIKANTFQYFVGAILLSIIAFLIVTPLSYMILALFRKKRG"
     gene            complement(3325..>3652)
                     /locus_tag="PKN22_07025"
     CDS             complement(3325..>3652)
                     /locus_tag="PKN22_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42495.1"
                     /translation="NKKKIKVKPSLFDLYINENQVGTVQLDQTIEIQPNGVFPIHVPI
                     TLNLQQGAIPVIIAGALKQTAKIRVVGKLKGSMYGITKSKKIDETKEISLKNLGDNKL
                     FNLSFF"
BASE COUNT         1155 a          610 c          647 g         1240 t
ORIGIN      
        1 aaacattatg gagtcacaat cgctgattta tctgctcatt ataaagacaa tgactttaac
       61 aaacaactaa gaaaaacatt agacaatagt tatttgggag ttactgtaac cgggacggct
      121 gttgttgctg ctgaaggtac acgttatttg atagacagtt tgtttgacgg aattttattt
      181 gctgtcataa cagtgagtgt attaatggca ttgctgttta gaaattgggg tatcgttttg
      241 gtttctatcg ttcctaattt aattccttta attttaactg gcggaataat gggcttgctc
      301 aatattccat taaaaccgtc tacattattg gttttcaata tttcatttgg aattacttct
      361 gatgattcta ttcacttttt agccaaatac cgtcaagagt taaagaaaaa cactggtgat
      421 attgccaaaa gtgttattga gtcattaaaa gaagtgggat tgagcatgtt ttacacgtcc
      481 attattctgt tcttcggatt ttccgtattc atgctttcac aatttggtgg cacaaaagca
      541 ttgggagttt tagtttctac aacccttatt tttgccattt ccatcaactt attgttggtg
      601 ccatcaattc tatacgacat tagtcgattg gtcaataaat ttaaaagaaa aaaagcagaa
      661 taaactattc gtaagaaatt acgaaaatat cttcattgtc ttttttaaac aattttttct
      721 cgcgcgcata gcgctcaatt ccattaagag tgtgcaactt ttcaagtgtt gctttggtat
      781 ctttcaacaa cgtctgtgta tcttcttttt gttgctcaat tttggaaagt ttcatgttct
      841 gacgtacaat cacaaaaata tctacatcgt ctaaaaacaa tgtataaata ccaaatacac
      901 ccaatgcaat gatgtattta ttttttagat actttggaat attcatcaca tcaaaaatag
      961 ctacaaatgt ttttagttgg aataagtttt tatttttcaa ttgacattct caggttaata
     1021 agtacatttg ttctataaat cctcgacgat gaatttttta aaactatcgt ttattcttgt
     1081 tttagttcaa ttatttgttg gatgcaatcc ttcggatgaa cgaaaaaaca aacttgtaat
     1141 ttccaaaatg ccccatcagg aactttcaat aaattggttg gaaggcttgt ggcaatgtga
     1201 tgataaagaa ggcgtattgt atgaggattg gaaaaaacta aacgatacgt tatttttagg
     1261 aaaaagctat tatttggcag atgatgacac attatttgta gaaaatatac agttaacact
     1321 tgttggcgat tcgctcagct attctagctt aaaaagagat acgttgcaaa aaaacaaatc
     1381 gtttaaaggt gttttatcaa ccaacaacgg atttagagtg gaaaatctac aacacggttt
     1441 tccgaaaagc atactttata acttgctcaa tgatacgttg atgatgattg aaatttctgg
     1501 tgaaattgat ggcttagttg aaacacaacc atatcaaatg gttaaaataa attctaaaag
     1561 ataatgaaat tggatgtagc acctatcgtt gaacaattga aaaatggagc caccatgctc
     1621 tatcccaccg atactatttg gggattgggc tgtgacgcca ccaatgaaac tgcttgccaa
     1681 cgtgttttag cactcaaaaa tcgcccagaa aataaaagct ttattgtgtt ggtagatagt
     1741 tttttgatgt tggaacgcta cattccagat tttaatccca tttgctacga tttagtggat
     1801 ttagctacgc gtccactcac cataatttat cccaatgcaa aaggacttgc gccatctgtg
     1861 ttggcagaag acggaagcgt tggcatacgc atcacaaaag atcctatttg cttgcaatta
     1921 attcgcgggt tgagaaaacc tattgtaagc acctcggcta atttgagtgg agagaaaagt
     1981 cccacttcat ttgcaaccat ttcaccacaa ataaaatctg gggtggactt aattgtggaa
     2041 caacgattga atgaaaaaat ggacaaagcg tcgcaaatca tcaaaattga attgaatgga
     2101 actgtgcatg taattcgtaa ataacttgta ggcacaattc ttattttctc aaattctttg
     2161 ttatttttgg atcaaattta acgccattgc aagcaaccat cgaacaaata aacgcctgtg
     2221 ttttaattcc cacatacaac aatgaaaaaa cgttgagaag ggttattgat ggagtgatgc
     2281 aacatgtttc tccaaaacac ataattgttg tcaacgatgg tgcaacagat gcaacctcaa
     2341 caattctttc cgactatcaa aatagcataa cagttttgaa caatgagaaa aatcgaggga
     2401 aaggttattc attgcgcaaa gggtttaaac atgccaatga aaaaggatat agttatgcca
     2461 ttaccattga ctcggatgga cagcattctc ccgacgattt acccaaaata atacaagcgt
     2521 gtttagaaca tccgggagct gttataatgg gctcacgcaa catgcaacaa gaaggtgttc
     2581 ctggaaaaag ttcttttggc aataaatttt ctaatttttg gttcaaagta gaaacactca
     2641 ttagtttgcc agatacacaa acaggatttc gtgcttaccc gcttccgcca ctaaaaaaaa
     2701 tgcgtttgtt taccacaaaa tttgagttag aaattgaggt gattgtgcgc ttggcatgga
     2761 aaggagtaaa atttattccc gttccaatta gtgtgaaata cgatcctaca gaacgtgttt
     2821 cgcattttcg tccgggtaaa gatttcttcc gcatttcggt gctaaacagt gtgttgttta
     2881 ttggtgctat gttatatttc tttcctaaaa aattattgtc caaaggtact tgggaagcca
     2941 ttaaacacga agccattaaa cccgaagaat ccaacatgcg caaagcagtt tcattaggat
     3001 ttggagtgtt tatggggatt tttcctattt ggggatttca attattggtt ggtattcctt
     3061 tggcaatatt aatgcgatta aataaaattt tgtttatcgc agcagccaat atttccattc
     3121 caccaatgat tccgtttatt atttatgcaa gttttttggt tggacaatat tttacagcgg
     3181 atgccattga tccagctcaa ttttttcact ttgatttaga tacaataaaa gccaatacat
     3241 ttcaatattt tgttggagct atattgcttt caattattgc atttttaatt gttactccgt
     3301 taagctatat gattttagct ttgtttagaa aaaagagagg ttaaaaagtt tattatcacc
     3361 caaatttttt aaagaaattt ccttggtttc atcaattttc ttgcttttgg ttattccata
     3421 catcgagcct ttgagttttc ccacaacccg aattttagct gtctgtttta acgctccagc
     3481 aataatcaca ggtatggctc cttgttgtaa atttaaagta ataggaacat gaattggaaa
     3541 aacaccattg ggctgtattt caattgtttg atccagttga acagttccaa cttgattttc
     3601 atttatatac aaatcaaata atgagggctt tacttttatt tttttcttgt tt
//
LOCUS       DAOQVQ010000211         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_29052_length_3631_cov_3.283557, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000211 DAOQVQ010000000
VERSION     DAOQVQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29052_length_3631_cov_3.283557"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PKN22_07030"
     CDS             complement(<1..750)
                     /locus_tag="PKN22_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNS42496.1"
                     /translation="MKLISFNVNGIRAITQKTFYKDMLDLNADVICLQETKATVEQTK
                     EALAQLSDYEVYANEAQRKGYSGTAIFTRVQPKNVFYDMGVEEHDQEGRITGLEFEDF
                     YLVTAYVPNSGSELLRLGYRQTWDAAFLAYLKELEKTKPVVVCGDLNVAHKAIDLARP
                     KENYNKAAGFMQEEIDGMDAYHANGLLDTFRAVNGDQVKYSWWSYRGGARQKNVGWRI
                     DYFLVSEQLLPKVKDAFILNEVMGSDHCPVGI"
     gene            complement(762..1664)
                     /locus_tag="PKN22_07035"
     CDS             complement(762..1664)
                     /locus_tag="PKN22_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS42497.1"
                     /translation="MKVVFQSIVPIFILAVALIACKKETITPQPDDTNTPPAVAVYDS
                     TQSYTLTDISYGNDPQQKMDIYLPANRSKDSTKVFILIHGGGWSSGDKADFVYFFNTL
                     KLYYPHHAIVNINYRLGTYLSPGYPKQIQDIQQAINHIQHPNYQLESEYFIFGNSAGG
                     HLGMFYGYRYDTLHQVKAICNTVGPADFTDVSYTGNSVYDYALSSFVGPITYAQNPTL
                     YAEVSPVKYITANSPKTISFYGDNDPLIPATQMGILHNELTTKGVYNESTQYAGEGHG
                     NWNQTNSNDYTAKIANFINQHFNY"
     gene            1793..3196
                     /gene="lpdA"
                     /locus_tag="PKN22_07040"
     CDS             1793..3196
                     /gene="lpdA"
                     /locus_tag="PKN22_07040"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNS42498.1"
                     /translation="MSNYDVTIIGSGPGGYVAAIRCAQLGMKTAIIEKYDTLGGTCLN
                     VGCIPSKALLDSSEHYHNAVHNFAEHGIQISAPKADIKQMIERKNGVVEQTCSGIKFL
                     MDKNKIDVFHGVGSFVNATTIKVTDSKGKETQITSKNTIIATGSKPNYFPGMEPDKKR
                     IITSTEALSLKEVPKKMIVIGGGVIGLELGSVYNRLGTEVEVVEFAPNIIPTMDGTMS
                     KELTRVLKKDGFKFYMEHKVQSVENKGKKVVLTALNKKGEEVKLEADYCLVAVGRKPY
                     TAGLGLENAGLKATDRGQIEVNNHLQTAVPNIFAIGDVVRGAMLAHKAEEEGVYVAEF
                     LAGQKPHINYDLIPGVVYTWPEVASVGKTEEQLKAEGANFKVGSFPIKALGRARASMD
                     TQGLVKILADKETDEILGVHMIAPRAADLIMEAVVAMEYRASAEDIARICHPHPTYAE
                     AVKEAALDAAFGKPIHV"
BASE COUNT         1147 a          614 c          770 g         1100 t
ORIGIN      
        1 aatgcccacc ggacaatggt cggaacccat tacttcgttg agaataaatg catcttttac
       61 ttttggtaat aattgttcgg aaactaaaaa atagtcaatc cgccagccaa cgttcttctg
      121 acgagctcca ccgcgataac tccaccaaga atattttacc tgatcgccgt ttaccgctcg
      181 aaaagtgtcc aacaaaccat tggcatgata ggcgtccatt ccgtcaattt cttcttgcat
      241 aaaaccagct gctttgttgt aattttcttt tggtcgcgct aaatcaatgg ctttatgtgc
      301 cacgtttaaa tcgccacaaa caaccacggg ttttgttttt tccaattctt ttaaataagc
      361 taaaaatgca gcatcccacg tttgacggta tcctaatcgc aacagttcgc taccagaatt
      421 gggaacataa gccgttacca aataaaaatc ttcaaattct aaacctgtaa tgcgtccttc
      481 ttggtcgtgt tcttccacgc ccatgtcgta aaacacattt ttaggttgaa cacgtgtaaa
      541 aatagctgtt ccagaatagc ctttgcgttg agcttcgttg gcatatactt cgtagtcact
      601 taattgcgcc agtgcttctt tggtttgttc aactgtagct ttggtttctt gtaaacaaat
      661 cacgtcggcg tttaagtcca acatgtcttt gtaaaatgtt ttttgtgtaa tggcgcgaat
      721 tccgttaacg ttgaaggaaa ttaatttcat ggatgttgct gttaataatt aaaatgttga
      781 ttgataaagt tagcaatttt agccgtgtaa tcatttgaat tggtttgatt ccagttgcca
      841 tgaccttcac ctgcatactg cgtagattcg ttgtaaacac ctttggtagt taattcgttg
      901 tgcaaaattc ccatttgggt agctggaatt aatgggtcgt tgtcgccata aaaggaaatg
      961 gttttaggtg aattggctgt aatgtattta acgggactca cctcggcata cagtgttggg
     1021 ttttgtgcat aggtgatggg tccaacaaaa cttgataaag cataatcgta cactgaattt
     1081 ccggtgtagg aaacatctgt aaaatctgct ggaccaaccg tgttgcatat agctttcact
     1141 tggtgcaagg tgtcgtaacg ataaccgtaa aacatgccca aatgtccgcc tgccgaatta
     1201 ccaaaaataa aatattcact ttcaagctgg taattaggat gttgaatgtg attgattgct
     1261 tgttgaatgt cttgaatttg cttaggatat cccggactca gataagttcc taatcggtaa
     1321 ttaatgttaa caatggcatg gtgtggatag tacaatttca acgtattgaa aaaatacaca
     1381 aaatcagctt tatcgcctga actccaacca ccaccatgaa tgagaataaa aactttggta
     1441 gaatctttag aacgattggc tggcaaataa atatccattt tttgttgtgg gtcattgcca
     1501 taggaaatgt ccgttagcgt gtaggattgt gtagaatcat ataccgccac agcaggaggt
     1561 gtgtttgtat catcgggttg tggggtaatt gtttcttttt tacaagcaat caatgcaact
     1621 gctaaaataa aaataggtac gatggattga aaaacaactt tcatggtttt aatttataac
     1681 gtaaatttaa taaaattgaa tgatgaaaat caaaattcga tgaaatcatc caaatttttc
     1741 ttccttctct cagtaaaagt cattacattt gtcgtctaaa atttgacaaa ttatgagcaa
     1801 ttacgacgtt acgattatag gttctggacc aggaggatat gtggcagcaa ttcgttgtgc
     1861 acagttggga atgaaaacag caattattga gaaatacgac acattgggtg gaacgtgctt
     1921 gaatgttggt tgtattccat ctaaagcgtt attggattca tcagaacatt atcacaatgc
     1981 ggttcataat tttgcagaac acggaattca aatcagcgca ccaaaagccg acatcaaaca
     2041 gatgattgaa cgtaaaaatg gagtggtaga gcaaacgtgt agcggtatca aatttttgat
     2101 ggacaaaaac aaaattgatg tttttcacgg agtaggttct tttgtgaatg caaccactat
     2161 taaagttaca gacagcaaag ggaaagaaac gcaaattaca tctaaaaata ccattattgc
     2221 aacgggttca aaaccaaatt attttccagg aatggagccc gataaaaaac gcatcattac
     2281 atcaacagaa gcattgagct tgaaagaagt tccaaagaaa atgattgtta tcggtggtgg
     2341 tgttattggt ttggaattag gatctgttta caaccgtttg ggaacagagg tggaagtagt
     2401 tgagtttgca cctaatatta tcccaaccat ggatggaacg atgtctaaag aattgacacg
     2461 cgttttgaaa aaagatggtt tcaaattcta tatggaacac aaggttcaaa gcgttgaaaa
     2521 caaaggtaaa aaagtggtgt tgacggcatt gaacaaaaaa ggggaggaag taaaattaga
     2581 agccgattat tgtttagttg ccgttggtag aaaaccatat actgctggtt tggggttgga
     2641 aaacgcaggt ttgaaagcta cagacagagg tcaaattgaa gtaaacaatc atttacaaac
     2701 agcagttcca aacatttttg caattggtga tgttgttcgt ggagcaatgt tggcacacaa
     2761 ggcagaagag gaaggggtgt atgttgctga atttttggca ggacaaaaac cacacattaa
     2821 ctacgattta attccgggag ttgtttatac ttggccagaa gttgcaagtg ttggtaaaac
     2881 agaagagcaa ttaaaagccg aaggtgctaa ctttaaggta ggttctttcc caatcaaagc
     2941 gttgggtagg gcaagagcat ctatggatac gcaaggttta gtaaaaatat tggcggataa
     3001 agaaaccgat gaaattttag gtgttcacat gattgcacca cgtgcagccg acttgattat
     3061 ggaagctgtt gtggcaatgg aataccgtgc atcggcagaa gatattgcgc gcatttgtca
     3121 cccgcaccca acgtatgcag aagcagtgaa agaagcagct ttggatgcag cgtttggcaa
     3181 accaattcac gtgtaagaaa aacaaataga tagattgaaa agagttgtcg aaaggcagct
     3241 cttttttgtt gggataaggc gtgttcaaaa aaaataaaaa gtgctacaaa cttccctact
     3301 tgagaaggga aagagggagg tgattgcaca catagatagc ttttcgcatt gtattatcca
     3361 cgattaaaat ttgtattttg atgatggtga gattctttta ttcatctcct taaatatcta
     3421 gtggaatatt aggaaaacaa ttgggtttta tgaacatgaa atgagttttt attattgggt
     3481 tgccttccaa tttttatttc tttacaaaag tcacctccct taatattttg attttaagaa
     3541 tagtatgtta atgatgagtg tgttgttaga atatctcagt tatttcaaaa gtcacctccc
     3601 ttaatccctc ctcaaggagg gagacatatt c
//
LOCUS       DAOQVQ010000212         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_29312_length_3609_cov_3.733821, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000212 DAOQVQ010000000
VERSION     DAOQVQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29312_length_3609_cov_3.733821"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..354)
                     /locus_tag="PKN22_07045"
     CDS             complement(34..354)
                     /locus_tag="PKN22_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNS42499.1"
                     /translation="MIVTTTNTIEGKSIKEYKGIVSGETIIGANIFKDFFAGIRDIVG
                     GRSASYEKVLREAKDIALQEMQQRAKELNANAILGVDLDYETVGGNGGMLMVTASGTA
                     VVIE"
     gene            complement(371..1228)
                     /locus_tag="PKN22_07050"
     CDS             complement(371..1228)
                     /locus_tag="PKN22_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNS42500.1"
                     /translation="MKLRVGGVPEHFNLPWKLALEDGEFKKEGIEVHWTDMPGGTGQM
                     LRGFENKTLDVAVMLTEGITKGILQGLNAKIVDVYVTSPLHWGIHVPFHSNYTKVEQL
                     ENQTFAISRMGSGSHLMAYVCADRMGWDTTNLKFNVIGDVYGGIWALEHNEAQLFLWD
                     KYMTHPFTTQEKCRYIDEVITPWPCFVIAAREEVIQENPEAIKLISKIVNERALVMKN
                     SEHIVEIISWRYNLRSNQVQNWLSETDWNYNLIKYPLAFEKTVRYLVKLNLITETEAE
                     NWREKLFFE"
     gene            complement(1239..3014)
                     /locus_tag="PKN22_07055"
     CDS             complement(1239..3014)
                     /locus_tag="PKN22_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS42501.1"
                     /translation="MSQQKKINIQVFFRLLSYANAYKKHYWIAIVSTLLLAILAPYRV
                     KLIGEVIDKYIVASPNAKLLLIWIAIILLMLVVEGVLQFVSSYFSNLLAQSVIYDIRV
                     KLFRQVTSFRMHFFNKHPIGNIVTRLVSDLEAITDVFSSGLMSVLGDILMLLITIVMM
                     FYADWELSLYVLVPIPLLIIGTRIFARAMRKSFQLESQQVAKLNAFVQERITGMNLVQ
                     LFNRQKQENAKFQEINAGHRTAHIKAIWANSIFFPYVEMLNSLSIAFLYIITVVGLTG
                     DTSADMSSKYGEIMAFTLWVNQLYRPIRMLADKFNILQRGTVRAERVFDLLDTGHQNV
                     QGEGKCIDCNFNQDITFNNLYFAYDDAKWILKNINLKIEAGSMVAFVGATGAGKTSIV
                     NLLGRFYDYQKGNIQIGDTEITDIDLKYLRRNIAIVLQDVFLFSDTIFNNITLRDASI
                     TKEQVIEAAKAVGAHEFIEKLPGGYDYQVGERGGVLSVGQRQLLAFIRAYVYNPQILI
                     LDEATSSVDNESELLIQKATEKLTSGRTSIVIAHRLSTIQKANKIVVMEKGEIVEQGS
                     HKELLQIEGKYKQLFDMQFGVENKV"
     gene            complement(3186..>3609)
                     /locus_tag="PKN22_07060"
     CDS             complement(3186..>3609)
                     /locus_tag="PKN22_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS42502.1"
                     /translation="KDDECPATPGPASNKGCPVIEEAVKEILKTAFDNLEFETGKDII
                     KEVSKPSLDELAGVLVKKPEWKLQIAGHTDNVGNAQSNLILSKKRAEAVKNYLSSKGV
                     DASRFSVLYFGQTMPIADNNTPEGRQKNRRVEMSIIFE"
BASE COUNT         1052 a          757 c          614 g         1186 t
ORIGIN      
        1 catcactcct ctttttgttt cattcagttt gcattactct attacaacgg cagttccact
       61 ggctgttacc atcaacattc caccattgcc tcccacagtt tcataatcta aatccactcc
      121 aagaattgca ttggcattta attctttagc tctttgttgc atctcttgca aagctatatc
      181 tttggcttca cgcaaaactt tttcatacga tgcagatcgt cctccaacaa tatcgcgaat
      241 tcctgcaaaa aaatctttga aaatattagc accaattatg gtttcaccag acacgatgcc
      301 tttgtattcc ttaattgatt ttccctcaat ggtattggtt gttgttacta tcatgttata
      361 aaaatttaag ctattcaaaa aataattttt ctcgccaatt ttctgcttct gtttccgtaa
      421 tgaggttcaa tttcactaaa taacgcaccg ttttttcaaa agcaagtggg tatttaatca
      481 aattatagtt ccaatcggtt tctgagagcc aattttgcac ttggttagaa cgtaaattat
      541 agcgccaaga aataatttcg acaatgtgtt cgctgttttt cataacgagt gcacgttcgt
      601 tcacaatttt agaaattagc tttattgcct ctgggttttc ttgaatcact tcttcacgtg
      661 cagcaatcac aaaacacggc caaggtgtaa tcacttcgtc gatgtaacgg catttttctt
      721 gggttgtgaa tggatgcgtc atgtatttat cccacaaaaa caattgagct tcgttgtgct
      781 ccaaagccca aattccacca taaacatcac caataacgtt gaatttaaga ttagtagtgt
      841 cccaccccat tctgtcggca caaacatatg ccattaagtg cgaaccagaa cccattcgcg
      901 aaatagcaaa cgtttggttt tctagttgtt ccacttttgt gtagttggaa tgaaatggca
      961 cgtgaattcc ccagtgcaaa ggcgacgtta cgtaaacatc tacaattttt gcgttcaatc
     1021 cttgcaagat tcctttggta attccttcgg taagcatcac agcaacatcc agcgttttgt
     1081 tctcaaatcc gcgcaacatt tgccctgtgc ctccaggcat atccgtccag tgcacctcaa
     1141 ttccttcttt tttgaattca ccatcttcca atgccaattt ccacggcaag ttaaaatgtt
     1201 cgggcactcc gccaactctt aatttcatac tttttatttt aaactttgtt ttcaacacca
     1261 aattgcatgt cgaaaagttg tttgtatttt ccttctattt gcaacagttc tttgtggcta
     1321 ccttgttcca caatttcacc tttttccatc accacaattt tattggcttt ttgaatggtt
     1381 gaaaggcggt gagcaattac gattgatgtt cttccgctgg tcaatttttc ggttgctttt
     1441 tgaatcaata gttccgattc attatccaca cttgatgttg cttcgtccaa aatcaatatt
     1501 tgcgggttgt aaacataagc acgaatgaat gctaataatt gacgttgccc caccgataat
     1561 acgccaccac gttcgcccac ttgataatcg tatccacccg gaagtttttc gataaattca
     1621 tgtgctccaa cggcttttgc agcttcaatt acttgttctt tggtgatact ggcatcacga
     1681 agtgtaatgt tattgaaaat agtatcggaa aataaaaata catcttgcaa cacaatggct
     1741 atgtttctgc gcaaatattt gaggtcgata tcggtaattt ctgtatctcc aatttgaata
     1801 tttccttttt gataatcgta aaatctgccc aataaattaa cgatggatgt ttttccagca
     1861 cctgttgctc caacaaaagc caccattgat cccgcttcaa ttttgaggtt gatgtttttc
     1921 aaaatccatt tggcatcgtc gtaagcaaaa taaaggttat tgaaagtaat gtcttggtta
     1981 aaattgcaat cgatgcattt tccttcgcct tgcacgtttt gatggccggt gtctaacaaa
     2041 tcaaaaacac gttcggctct cacggttccg cgttgtaaga tgttgaattt atccgccaac
     2101 atgcgaatag gacgatacaa ttgattgacc cacaatgtaa acgccatgat ttctccgtat
     2161 tttgacgaca tatcggcaga ggtgtcaccc gtgagtccaa caacggttat gatgtacaaa
     2221 aaagcaatag aaagtgagtt gagcatttcc acataaggaa aaaagatgga gttggcccaa
     2281 attgctttga tgtgtgcggt gcggtgtccg gcgtttattt cttgaaattt tgcattttct
     2341 tgtttttgtc ggttgaacaa ttgcacaagg ttcattcccg ttatgcgttc ttgcacaaag
     2401 gcgtttaatt tagccacttg ttgactttcc aattgaaacg atttgcgcat agctcgagca
     2461 aaaatgcgtg ttccaataat caacaaaggg atgggcacca aaacatagag cgataattcc
     2521 caatcggcat aaaacatcat cactatggtt ataagcaaca tgagtatatc acccagcaca
     2581 ctcattaatc ctgatgaaaa cacatcggtg atagcctcta aatcagaaac tagtcgtgtt
     2641 acaatatttc caatgggatg tttgttaaaa aagtgcattc gaaacgaggt aacttgccgg
     2701 aaaagtttca ctcgaatgtc gtaaataacc gattgagcca acaagtttga gaaataggaa
     2761 gaaacaaatt gcaatacgcc ttccacaacc aacatcaata ggatgattgc aatccaaatc
     2821 aacaacaatt ttgcgttggg cgatgcaacg atgtatttgt caattacctc gccaattaat
     2881 tttacgcgat agggagctag gatagccaac aacaacgtcg aaacaatagc tatccaataa
     2941 tgttttttat aggcattggc atagctcaat aagcgaaaaa atacttgaat attgattttc
     3001 ttttgttgcg acataaggaa tacaaagtta tcaaaaatag ggagctatta gatttaataa
     3061 catcaaatta acgaaagatt ttaggctttt ttatcatatc gacatatttc gatatagcga
     3121 tatcaccaac aaaaaagggc ttgaagtttt acctccaagc cctttatgaa ttttaaatga
     3181 ttgaattatt caaaaataat tgacatttca actcgtctat ttttctgacg accttctggc
     3241 gtgttgttgt cagcaattgg cattgtttga ccaaaataca atacactaaa tctacttgcg
     3301 tccactcctt tagaagataa gtagtttttc actgcctcag ctcttttctt agaaagaatc
     3361 aagttgcttt gtgcattacc tacgttatct gtgtgaccag cgatttgtaa tttccattct
     3421 ggttttttca ccaatacacc agccaactca tccaaagatg gttttgaaac ttctttaatg
     3481 atgtctttac cagtttcaaa ttccaagtta tcaaatgctg ttttcaagat ttctttaaca
     3541 gcctcttcaa taacaggaca acctttattg gatgctggtc caggtgttgc cggacattca
     3601 tcatcttta
//
LOCUS       DAOQVQ010000213         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_29502_length_3591_cov_3.926188, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000213 DAOQVQ010000000
VERSION     DAOQVQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29502_length_3591_cov_3.926188"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PKN22_07065"
     CDS             complement(<1..1158)
                     /locus_tag="PKN22_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42503.1"
                     /translation="MLRQLLFRNQDKKQLIIAIVGSFLGITFLITSIHYLIKVNEFGK
                     GTEILGPNTLIVQKKVNAGAIMGLAKTDFSDSEIEKMKKERFISDVKPVISNNFDVSF
                     QTDDPHVPYFRSDVFIQTVDPQFLDVKSDKWKWKEGDTVVPIILPRDFIVMLNTFMSA
                     SGIPQVSDELAKEIRFKFTIKNEERKEFIAAKNIGFTNEVASILVPQSFMEYGNKNFA
                     LGADKKITQIMIAGKESEFGLVEEYLVKHGLESKNSQMVVGKLKSIVSTLIVVILGIS
                     IIAVFVSCLVLIQYLQLLMSKNAYEVRTLIRIGYPPHSIVKTFFVYFLKIFGIITLSG
                     FAVFFIFKFFLDNILIAGGLAISSTVSLYSIIALVVAYCIFAWASYSTAKRG"
     gene            complement(1162..1803)
                     /locus_tag="PKN22_07070"
     CDS             complement(1162..1803)
                     /locus_tag="PKN22_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS42504.1"
                     /translation="MKISLNKVMPIPLASINHGANSIWGNEVELLPGKKIMLNAASGK
                     GKSTFTYTTTGLRNDYSGAILYDDRDVKTFSVEEWTTIRQTKISTVFQDLQLFPTLTV
                     KENLLLKNKLTSVFSESELKEKLDQLGIADKWEQRCGLLSMGQQQRVAIIRALAQPFE
                     WLIMDEPFSHLDEANTQLCLAMINKRADELNAGFVLTSLGDDHAFQYDYTLNL"
     gene            complement(1874..3241)
                     /locus_tag="PKN22_07075"
     CDS             complement(1874..3241)
                     /locus_tag="PKN22_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS42505.1"
                     /translation="MKSILLIVVVVLSVVAHAQTADSAFIRRIYDKALTESEAYSNLR
                     YLCKNIGHRISGSAAAQQAVEWSLKLMDSYGFDNAYLQPVMVPYWKRGTKEEGWYKTG
                     MGELQKVHLLALGGSVGTNGTIEAEIVEFKTLEDLKKATKAQVEKKIVFVNEPMNSKH
                     ISTFNAYGGCVSIRAYSAEEAGKLGALAVIIRSVGMPIDEHPHTGSMYYGEDAKVKIP
                     AAAISTQDAENLSALLQQQKVKFVLRMDCEYVGMVQSFNVIAEMKGTVHPNKIITVGG
                     HMDSWDTGEGAHDDGAGLMHSLEALRILKNLNYKPKNTLRVVFFMNEENGNYGGEMYA
                     KKAKESGEEHIAAIESDAGGFVPRGFHLDGNSQQLAFLQSLAPVLKPYDLHVFEKGYG
                     GVDIGPLKRQFDSIPLFGFIPDSQRYFDVHHSPNDVFETVNKRELELGCAAIASLLYL
                     IDANF"
     gene            complement(3254..>3591)
                     /locus_tag="PKN22_07080"
     CDS             complement(3254..>3591)
                     /locus_tag="PKN22_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42506.1"
                     /translation="LSLFALPFSLAAVTLLYAPWSSEWQCARGIHYHQQQDFLTAETY
                     YKKALSIDSQNKLAKENYTLIQVDRLSKQAYKAHMAKDYTDARRLYLKILALKKNNHW
                     ARENLKELP"
BASE COUNT         1142 a          777 c          546 g         1126 t
ORIGIN      
        1 tccacgtttt gcagttgaat aactcgccca agcaaatata caataagcca caacaagtgc
       61 tataatgctg tataaactta ccgttgaact gatggctaat ccacctgcaa tcaaaatgtt
      121 atctaagaaa aatttaaaga taaaaaatac agcaaatcca ctcaaggtga taatcccaaa
      181 aatctttaaa aagtaaacaa aaaacgtttt cacaatggaa tgtggcggat atccaatacg
      241 tatcaacgtg cgaacctcgt atgcattttt agacatcaac aactgcaaat attgaatcaa
      301 taccaaacaa gacacaaaca ccgcaattat agaaatacct aaaataacga caatcaacgt
      361 gctgacaata cttttcaatt ttcctaccac catttgtgag tttttagact ccaatccgtg
      421 ttttaccaaa tattcttcca ccaagccaaa ctcactttct tttccagcaa tcattatctg
      481 agtgattttc ttgtcagctc ctaatgcaaa gttcttgtta ccatattcca taaacgattg
      541 aggcaccaaa atagaggcta cttcattggt aaacccaata ttttttgcag caataaattc
      601 ttttctctcc tcgtttttga tggtaaattt aaaacggatt tcttttgcca attcgtcaga
      661 tacttgcgga attccactag cgctcataaa cgtgttgagc atcactataa aatcacgcgg
      721 taaaataatt ggaacaacgg tatcgccttc tttccacttc catttgtccg attttacatc
      781 caaaaattgt gggtcaacag tttgaataaa aacatccgaa cgaaaatagg gaacatgcgg
      841 atcatcggtc tgaaacgaca catcgaaatt gttcgatatc accggtttta catccgaaat
      901 aaaacgctct tttttcattt tttcaatttc tgaatctgaa aaatcggttt ttgccaatcc
      961 catgatggct cctgcattca cttttttctg aacaattaac gtgttgggtc ccaaaatttc
     1021 tgttcccttg ccaaattcat tgactttaat caaataatgg atagacgtta tcaaaaacgt
     1081 aatgcccaaa aacgaaccaa caatggcaat aatcaattgt tttttatctt gatttctaaa
     1141 taataactgg cgtaacatgg ctcaaagatt taatgtgtaa tcgtattgaa atgcgtgatc
     1201 atctcctaaa gacgtcaaaa caaaacctgc atttagttca tctgcacgtt tgttaatcat
     1261 cgctaaacac aattgtgtat tggcttcgtc taaatgagaa aaaggttcgt ccataatcaa
     1321 ccattcaaaa ggttgcgcca atgcacgaat aatggctaca cgctgttgct ggcccatgga
     1381 caacaaacca catcgttgct cccatttatc ggcaattcct aattgatcca atttttcttt
     1441 caattcactt tcagaaaaaa cagaggtcaa cttatttttg agcaataaat tttctttgac
     1501 cgttaaggtt ggaaacaact gtaaatcttg aaaaaccgta gaaatttttg tttggcgaat
     1561 cgttgtccac tcctccacag aaaaggtttt tacatctcta tcatcgtaaa gaatcgcacc
     1621 cgaataatca tttcgcaaac ctgttgttgt atatgtaaaa gtagatttcc cctttccact
     1681 tgcggcatta agcatgattt ttttcccagg caacaactcc acttcattcc cccaaatact
     1741 atttgcccca tgattgatgg acgctaaggg aatgggcatt actttgttta aactaatttt
     1801 cattttgcat tgtttgaaaa gtgcaaggca attattgttc caaaaagaaa atagttgtgc
     1861 aaactactaa aaatcaaaag ttagcatcaa tcaaatagag caacgacgca atggctgcac
     1921 aacctaattc caattcgcgt ttgttaaccg tttcaaaaac atcattgggt gaatgatgta
     1981 catcaaaata gcgttgtgaa tcgggaataa aaccaaataa tgggatggaa tcaaattgac
     2041 gtttgagtgg accaatatca acaccaccat atcctttttc aaacacatgc aaatcgtatg
     2101 gttttaaaac tggagctaaa ctttgcaaaa aagccaattg ttgcgaattg ccgtccaaat
     2161 gaaatcctct aggcacaaac ccacccgcat cactttcgat agccgcaatg tgttcttcgc
     2221 cactttcttt tgctttttta gcatacattt ctcctccata atttccattt tcttcgttca
     2281 taaagaaaac aacacgcaaa gtatttttag gtttataatt taagttttta agaatgcgta
     2341 atgcttctaa actatgcatt aatcctgcac catcatcgtg tgctccttcg cctgtatccc
     2401 aagaatccat atgtccacca acagtaatga ttttattcgg atgtactgtt cctttcattt
     2461 ccgcaatcac attaaacgat tgcaccatcc ccacgtattc acaatccata cggagcacaa
     2521 atttcacttt ttgttgttgc aataacgccg ataaattctc agcatcttgt gtggatattg
     2581 cagcagcagg tattttcacc tttgcatctt ctccataata cattgaaccc gtgtgtggat
     2641 gttcatctat gggcattcca actgaacgaa taatcactgc caaagcaccc aatttacctg
     2701 cttcctctgc gctatacgca cgtatagaaa cacaacctcc ataggcatta aatgtactga
     2761 tatgtttcga attcatcggt tcgttgacaa acacaatttt cttttcaacc tgtgcttttg
     2821 ttgctttttt taaatcttcg agtgttttaa actctacaat ttccgcttca atggtaccgt
     2881 tagtgcctac acttcctcca agcgccaata aatgaacctt ttgaagttca cccatcccag
     2941 ttttatacca accttcttct ttggtccccc ttttccaata aggcaccatc actggttgaa
     3001 gataagcatt gtcaaaccca taagaatcca tcaatttaag cgaccattca acggcttgtt
     3061 gagcagcagc agaacccgaa atacggtggc caatattttt acacaaataa cgcaagttag
     3121 aataagcttc actttcggtt aaagctttgt cataaattct tcggataaat gctgaatcag
     3181 cagtttgtgc atgtgctaca acagatagta caaccacaac tattagtaaa attgatttca
     3241 taaacaaaag attttatggc aattctttta agttttctct tgcccaatgg ttattttttt
     3301 tcaacgccaa aattttcaaa tacaagcgtc gtgcatctgt atagtctttt gccatgtgtg
     3361 ctttataagc ttgtttagag agacgatcta cttgaattaa cgtataattt tctttggcta
     3421 atttattctg actatcaata ctcaatgctt ttttgtaata ggtctcagcc gttaaaaaat
     3481 cctgttgctg atgataatgg attccacgtg cgcattgcca ttcactcgac caaggagcat
     3541 aaagcaaggt aacagcagcc aatgaaaatg gcaaagcaaa tagagataaa t
//
LOCUS       DAOQVQ010000214         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_29638_length_3580_cov_4.360284, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000214 DAOQVQ010000000
VERSION     DAOQVQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29638_length_3580_cov_4.360284"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3580
                     /locus_tag="PKN22_07085"
     CDS             <1..>3580
                     /locus_tag="PKN22_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNS42507.1"
                     /translation="LLGRLFTFFVSDVTNVAVWINTMSALSSSFTILFMFWSITLLVK
                     KMVLERKKELSSGDVIAIMGSGVVGALAYTFSDTFWFSAVEGEVYAMSSLFTAIVFWA
                     ILKWDEEMALLQGGKLSIEAAPNRWLLFILFMIGLAIGVHLLGILLVPAIGYVIYFRL
                     WKEIKAKTFILVGIISVVVLGVIQEGVIPGTISFASSFEIFFRNSIGLPFLSGTIFFF
                     ALLIGGLIYGARYARKKGKTVLYNSIFALIFLFIGYGSFAVIVIRSNADTPMDQNNPE
                     NLVNLRSYLKRDQYGAAPLLSGPYWNSKELGGRFDGDGTWDAYADRSGWGDRDAVYQR
                     RFIAQKNEQNLKTFLKKEDAEKYAKENGAEVKELFYEVNADSRLKSEPAYQQSTIFPR
                     MYSPDEPRKVEGYKYWSNYDASRRDGEMGKDNLPLPTFGENLKYFFSYQCNFMYFRYF
                     MWNFAGRQNDIQGDGNQMAGNWKSGISFVDDARLGDQSKAPYYTTNNKSNNSLFFMPL
                     ILGLIGLFFHAYRSPKDAFVVFLGFLLTGLAIVVYLNQKVYEPRERDYAYAGSFYFFA
                     IWIGFGVYALYHAFTSFTKEHFKKAGVIALAGLVLFFIFDLGSEASNPNTRAWLVIAL
                     IGLLMLGIMRVLGKVLKSENAGAVLATVLGLIAPVIMGAQEWDDHDRSGKTTAHDVAY
                     NYMSSVSPNGIIFTNGDNDTFPLWYIQEVEGFRSDVRVCNLSLMQTDWYTEQMMRKSY
                     ESDPLPIKFTRDQVLMYTGGTDQIQFVTLTDLYFSNSASAETLRSVIEMRLKNNKSAA
                     INAINGFASSMSTVIGSLEVTQPGAIQRVNQLKAILVRPAQDADLVNDIHARFTAMRE
                     IFNALQNRVITANESAIQGVQNGLQQLNAGWESMDLTEVMAFVRNDANMLTTQANTFR
                     FFPTNTFSLKVNKENLLKSGVVPSNTDKSKIMDQVQFTLSARAITREEIMMLDVLANN
                     DWKRGIYFSSPYGSNVSKALLNAGALKQTGSTYEVNPIQQEAPMDVEEMYESLMKKQH
                     FGNMKDPNVLTDYYARRHVVQYRSQFASLVKYYLNSNDNIKRAQSYPEEYLKTSAQAG
                     NADAAKLLELRKNADQTIAENNKHISALLNRAEEVMPIDVVLDSGEPGMVGSFKYQGS
                     EYPRYSDGVVSDFVEMYYSAGDKKQGDALGLKMEKQLRQAIEYFLTC"
BASE COUNT         1063 a          581 c          788 g         1148 t
ORIGIN      
        1 gttgttggga cgtttgttta ccttttttgt tagcgatgta accaatgttg cagtatggat
       61 taacaccatg tcggcacttt ctagctcatt taccattctt tttatgttct ggtccattac
      121 cttgttggtg aaaaaaatgg ttttggaacg caaaaaagaa ctttcaagtg gcgacgtgat
      181 cgctattatg ggaagtggtg ttgttggagc cttggcttat acattttctg atacgttttg
      241 gttctcggca gtagaaggtg aggtgtatgc catgtcatct ttgtttactg ccattgtgtt
      301 ttgggctatc ttgaaatggg acgaggaaat ggccctgttg caaggaggaa aactatccat
      361 tgaagcagct ccaaatagat ggttattgtt catcttgttt atgattggtt tggctattgg
      421 ggtccactta ttgggtattc tattggtgcc agctatcgga tatgtaatct atttccgttt
      481 gtggaaagaa attaaagcta aaacatttat cttagttggt atcatttcgg ttgttgtttt
      541 aggtgttatt caagaaggag ttattcctgg aacaatatct tttgcttctt cgtttgagat
      601 tttctttaga aattcaatag gtttaccgtt cttatcgggt accattttct tctttgcctt
      661 gttgattggt ggattgattt atggtgcacg ctatgcccgt aaaaaaggaa aaacagtatt
      721 gtacaacagt atttttgctt tgattttctt atttattggc tatggttctt ttgccgttat
      781 cgtgattcgt tccaatgcag atacaccaat ggaccaaaac aacccagaaa acttggtgaa
      841 cttacgttcc tacttaaaac gcgatcaata tggtgctgct ccacttttat ctggacctta
      901 ttggaattct aaagaattag gcggacgttt tgatggagat ggaacttggg atgcttatgc
      961 agaccgttct ggttggggcg atagagatgc tgtttatcaa cgtcgcttta ttgctcaaaa
     1021 aaatgaacaa aaccttaaaa catttttgaa aaaagaagat gcagagaaat atgccaaaga
     1081 aaatggagct gaggttaaag aattgtttta cgaagtaaat gctgattcac gtttaaaatc
     1141 agaaccagct tatcagcaat ctactatttt cccacgtatg tatagcccag atgaaccgcg
     1201 taaagtggag gggtataaat attggtccaa ctacgatgct tcacgcagag atggagagat
     1261 ggggaaagac aatttgccgt tgccaacttt tggtgaaaac ttgaagtatt tttttagcta
     1321 tcagtgtaat ttcatgtatt tccgttattt tatgtggaac tttgcaggtc gtcaaaatga
     1381 tattcagggc gatggtaacc aaatggcggg taactggaaa tctggaattt cttttgtaga
     1441 cgatgcacgc ctaggcgatc aatctaaagc accttactat acaaccaata ataaatccaa
     1501 taacagcttg tttttcatgc cactcattct tgggttgatt ggtttatttt tccatgctta
     1561 tcgttctcct aaagatgcat ttgtggtttt cttaggattc ttgttaactg gtttggcaat
     1621 tgtggtttat ttgaatcaaa aggtttatga gccaagagaa cgtgattatg cgtatgctgg
     1681 ttcgttctat ttctttgcta tttggatagg ctttggtgta tatgcacttt atcatgcatt
     1741 tacatcattt accaaagaac atttcaaaaa agctggagta attgcattgg caggtttggt
     1801 gttgttcttc atttttgact taggaagtga ggcttctaac cctaacacac gcgcgtggtt
     1861 ggtaattgca ttgattggtt tgttgatgct tggcatcatg cgtgttctgg gcaaagtatt
     1921 aaaaagtgaa aatgcagggg cagtacttgc aacggtgctt ggactaattg ctccagtgat
     1981 tatgggggct caagagtggg atgaccacga tagaagcgga aaaactacag cgcacgatgt
     2041 tgcctacaat tacatgagtt cggtttctcc aaacggaatt atttttacca atggtgataa
     2101 cgataccttc ccattgtggt atattcaaga agttgaaggt ttccgctcgg atgtgcgtgt
     2161 atgtaactta tcgttgatgc aaacagattg gtacaccgaa caaatgatgc gtaaaagcta
     2221 tgaatcggat ccattgccaa tcaagtttac aagagatcaa gtgttgatgt acacaggtgg
     2281 cactgaccaa attcagtttg ttactttaac cgatttgtat ttctctaatt cggcatcggc
     2341 tgaaacattg cgaagcgtca ttgaaatgcg tttgaaaaac aataaatcag cagcaattaa
     2401 tgcgattaat ggttttgctt cttctatgtc aaccgtaatt ggaagtttag aagtaacaca
     2461 acctggggca attcaacgag ttaaccaatt gaaagctatt ttggttagac cggctcaaga
     2521 tgctgattta gtaaacgata tccatgcacg tttcactgca atgcgcgaaa tatttaatgc
     2581 gttgcaaaat cgtgttatca cagccaatga aagtgcaatt caaggggttc aaaatggatt
     2641 gcaacaatta aatgcaggat gggaatctat ggatttaaca gaggtaatgg catttgtacg
     2701 taacgatgct aatatgttga caacgcaagc aaatactttc cgtttcttcc ctaccaacac
     2761 gttctcttta aaagtaaata aagaaaactt attgaaatcg ggggttgtac catccaatac
     2821 agataaatca aaaatcatgg atcaagtgca gtttacattg tctgcacgag caattactcg
     2881 cgaagaaatc atgatgttgg atgtattggc aaacaacgat tggaaacgag gaatttattt
     2941 ctcaagtcct tatggaagca atgtttctaa agcgttgtta aatgcggggg cattgaaaca
     3001 aacaggttct acttatgaag tgaatcctat tcaacaagag gcgccaatgg atgttgaaga
     3061 aatgtatgaa agtttgatga aaaaacaaca ttttggcaac atgaaagatc caaacgtgtt
     3121 gacagattac tacgctcgtc gtcacgttgt tcaatataga agtcaatttg cttcattggt
     3181 gaaatactat ttgaatagca atgataatat caaacgcgca caatcgtatc cagaagaata
     3241 tttaaaaaca agtgctcaag ctggaaatgc ggatgctgct aaattgttag aattgcgtaa
     3301 aaatgcagat caaactattg ctgagaacaa caagcatatt tcagcattgt tgaatagagc
     3361 agaagaagta atgccaattg atgtggtgtt ggatagtgga gaaccaggta tggttggatc
     3421 attcaaatac caaggttcag aatatccacg ttattcagat ggtgttgttt ctgatttcgt
     3481 agaaatgtat tacagtgcag gagataaaaa acaaggcgat gcattgggct taaaaatgga
     3541 aaaacaattg cgccaagcta ttgaatattt cttgacatgt
//
LOCUS       DAOQVQ010000215         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_29916_length_3557_cov_4.501428, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000215 DAOQVQ010000000
VERSION     DAOQVQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29916_length_3557_cov_4.501428"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2165)
                     /locus_tag="PKN22_07090"
     CDS             complement(<1..2165)
                     /locus_tag="PKN22_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42508.1"
                     /translation="MIAFILAIDLSFGQCPSGASPYSGQTTIQSNEVFCMTADLTVAS
                     LTIASGGKLYVSNGKKLTVSGNVVVSGTLQLANEASVSMNKLTIGNGTVSNALVNLGK
                     KAYLKITNSLVQTPSTSPFVSKLEMWSGSVVENCGTHHQGVIDYPFIKYLGSESVKSY
                     YIVKKAVTGAGAGAHFSHDNNIVVIAMHSVSGVTAGLATYCGEYAEPAYCPSWPSGLS
                     NNPNECNNVTTILANLPEPETACSENTFTKSTDPNTIEYDNIVCGFHATILKESNGDI
                     KVWGDEANANGTSSVTAPRLVTPANGYNYTGSPLRVTMGSYAGSSLTSVVHQFALLTT
                     TGLYVWGKPGTLVHSDIKGNSAFGSITINGKNDGLPAGVSPSDVKMLFGSYKTLAIVT
                     YSGHAWVLSFNGSKNGDGSNQDEDNNNKKWHRVKTSNTTNLNNVVAVRGNTNALVALT
                     SDGKVYTWGSKTYANDNNGPSSRTYATEITLPSGLSTKMIGMAPNGNYNSYYVLGTNG
                     QLWAMGNNDVRQLGNFSTSNSNTWVRVKSASNTNMENIAWFSPQEHDNNYAAVNALTS
                     SGKLYSWGSNDGQMIGMGSATSSNPVYMGRGLNNNDVLMAVETGGHTTMIVRQCTFKY
                     GYLGHRVDGSMGDGTDVNTNESEFNFSNTAIINLCGAPTAPITKDLKMCEGLGESVNL
                     QDAFIGSVPAGENLVWQDENGNPVTNFVISEEKTFNAVFT"
     gene            complement(2380..>3557)
                     /locus_tag="PKN22_07095"
     CDS             complement(2380..>3557)
                     /locus_tag="PKN22_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42509.1"
                     /translation="LDSIAWFSPQEHDNNYAAVNALTVDGKLYSWGSNAGQMIGMGGA
                     TASNPFYMGRGLAANDYLLAVETGGHITMIVRECTFRYGYLGHRVDGSMGDGTDDNTN
                     ESIFNFSSTAVVNLCGAPTAPITKDLKLCQGLGQTVNLQDAFIGDVPAGESLVWLDSN
                     GDPVTNFIMDSIGTYSAVFQGGVCPVDPSSVVNVSFYTPADVPMYNLCLTILGKDLTA
                     FNAQCKDGFVDLNWTTASEKNLNRFEVERSTDGIKWELVSRVFATGDSHYEKLYSARD
                     FTNSQTTYYRLKIVDNDDHYERSYPIVSQCQSSGVSCTVFPVPASDFTTVTIGADEDE
                     QVNLLITDVDGRNVLQQAVKLQSGTNSFPLDISHLKNGIYFLSTDQKDFTPVKLVVQ"
BASE COUNT         1125 a          753 c          563 g         1116 t
ORIGIN      
        1 gtaaatacgg cattaaatgt tttttcttct gaaataacaa aattggttac cggatttcca
       61 ttttcatctt gccaaactaa attttctcca gctggaacac ttccgataaa tgcgtcttgc
      121 aaattaaccg actcacccaa tccttcacac atttttaaat ctttagttat aggagcagtt
      181 ggagcaccac acaagttgat aattgctgta ttggagaagt taaattcact ttcattggta
      241 ttcacatccg ttccatctcc catacttcca tctactcggt gacccaaata accgtatttg
      301 aatgtacatt gacgaacaat catagtagtg tgccctcctg tttcaactgc cattaacacg
      361 tcattattat tcaatccacg ccccatataa actgggttcg atgaagttgc actacccata
      421 ccaatcattt gaccatcatt actaccccat gaatacaatt tacctgagga tgtcaaagca
      481 ttcacggcag cataattatt atcgtgctct tgcggactaa accatgcaat gttttccatg
      541 ttagtattgg atgcgctttt tactcttacc catgtattgc tattactcgt agagaaattc
      601 cccaattgac gaacatcatt attgcccatt gcccacaatt gaccattggt gcctaacaca
      661 taataagaat tataattacc atttggtgcc attcctatca ttttggtaga taatcctgat
      721 ggcaaagtaa tttcggttgc gtatgttcta ctggaaggtc cattattatc atttgcatag
      781 gtcttagaac cccaagtata aacctttcca tcggatgtta acgctactaa cgcattagtg
      841 tttccacgca cagcaaccac attattcaaa ttagtggtgt tggatgtttt tacacggtgc
      901 cattttttat tgttattatc ttcatcttga ttagagccat ctccattttt acttccattg
      961 aaagacaaca cccaagcatg accagaataa gttacaatag ccaatgtttt gtatgatcca
     1021 aaaagcattt tgacatcaga aggtgaaact ccagctggca aaccatcgtt tttaccatta
     1081 attgttatag aaccaaaagc agaatttcct tttatatctg aatgaaccaa agtaccaggt
     1141 ttaccccaca catacaaacc agtggttgtc aataaagcaa attggtgaac tacagaagtt
     1201 aatgagctac cggcataact tcccattgtt actcttaaag ggcttccagt ataattatat
     1261 ccatttgcag gagtaaccaa cctaggagca gtaacagaag atgttccatt tgcatttgct
     1321 tcatctcccc aaactttaat atcaccgttg ctttctttta gaatagttgc atgaaaacca
     1381 caaacaatgt tgtcgtattc aatagtgttg gggtctgtac tttttgtaaa tgtattttca
     1441 gagcaagcag tttctggctc tggtaagttt gctaaaatag ttgtaacatt gttacactca
     1501 tttggattgt tactcaaacc acttggccaa cttggacaat atgcaggttc agcatattca
     1561 ccacaataag ttgctaaacc agctgtaact ccagaaaccg aatgcattgc aatcacaaca
     1621 atgttattat cgtgcgagaa atgcgcacca gctccagcac ctgtaactgc ttttttaaca
     1681 atgtagtatg attttacaga ctccgaaccc aaatatttga taaaagggta atcaataact
     1741 ccttggtggt gtgtgccaca attttcgaca acagaaccag accacatttc taatttagaa
     1801 acaaatggac ttgtagatgg tgtttgcact aaactgttgg taatttttaa ataggctttt
     1861 ttacctaagt taacaagtgc gttgctaaca gttccgtttc cgattgttag cttattcata
     1921 gaaacacttg cttcgttagc taattggaga gtgccactaa ctacaacatt tccagaaact
     1981 gttaactttt ttccattaga aacatacaat tttcctccag atgcaatcgt taaactagca
     2041 acagtcaaat cagctgtcat acagaacacc tcatttgatt gaatggtagt ttgtcccgaa
     2101 tacggagaag ctcccgatgg acattgtcca aaagataagt cgatagccaa tatgaacgct
     2161 atcataaaat agaatacact ttttacttta aacataggta ttagaaattt aatcgataaa
     2221 ctaaaatttt gtaaaaaaaa gtgcaaaagt acatatttca ttttaattat ttaacatcaa
     2281 cttaataagt gagttatttt aattaataaa tgagtaaaat gcataaaaaa accccgacac
     2341 aattgcatcg gggttaaagt atatatgaag aaaacttctt tattgcacca ccaattttac
     2401 aggagtaaaa tctttttggt cggtgctcaa gaaatatatg ccatttttaa gatgtgaaat
     2461 atccaaagga aacgaatttg ttccactttg caatttcacg gcttgttgca acacatttct
     2521 tccatctaca tcagtaatga gtaaattgac ttgttcatct tcatctgctc ctatggttac
     2581 ggttgtaaaa tccgatgctg gaacagggaa aacagtgcaa gaaacacctg aagattgaca
     2641 ttgcgaaaca ataggataag aacgctcgta atgatcgtcg ttatctacaa ttttcaaacg
     2701 gtaataagtt gtttgactgt ttgtgaaatc gcgtgctgaa tagagttttt cataatgaga
     2761 atcgccagtt gcaaacacac gtgacaccaa ctcccattta attccatcag ttgaacgttc
     2821 aacttcaaaa cggtttaagt ttttctctga agcagttgtc caattcaaat ccacaaaacc
     2881 atccttgcat tgcgcgttaa aggcagttaa atctttgcct aaaattgtta agcacaaatt
     2941 atacataggc acatctgcag gcgtgtaaaa agacacattt acaacagatg atggatcgac
     3001 tggacaaaca cctccttgaa acaccgcact atatgttccg atgctatcca ttataaaatt
     3061 ggttacagga tctccgttag aatctaacca aaccaagctt tcacctgctg gcacatctcc
     3121 aataaatgca tcttgcaagt taacggtttg ccctaatcct tgacacaatt ttaagtcttt
     3181 tgtaattggt gctgtaggtg caccacataa attcacaaca gctgtactcg agaagttaaa
     3241 aatactttca ttggtattat catccgttcc atcccccata cttccatcca ctcggtgacc
     3301 taaataaccg tatctaaatg tacattctct aactatcatc gtgatgtgtc cacctgtttc
     3361 aactgccaat aaataatcat ttgctgctaa gcctctaccc atgtaaaaag gatttgaagc
     3421 agttgctcct cccataccaa tcatttgacc agcattgctt ccccatgaat acaatttccc
     3481 atctacagtc aatgcattca cagctgcata attattatcg tgttcttgtg gactgaacca
     3541 tgcaatactg tctaaaa
//
LOCUS       DAOQVQ010000216         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_29952_length_3554_cov_4.243784, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000216 DAOQVQ010000000
VERSION     DAOQVQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29952_length_3554_cov_4.243784"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1668)
                     /locus_tag="PKN22_07100"
     CDS             complement(247..1668)
                     /locus_tag="PKN22_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNS42510.1"
                     /translation="MKSRYYNLVIGIATVGMLSGCGVITKKYEKPTISEEQQKGLFRD
                     HTSQDTTTLADVKWQDFFQDEHLKALIQEGLDKNYDLQNAILQISTAENTLKQSKQAF
                     APSLDFTPQVTYNKMSQNALNFPSNVNINLQTTSVSLGFSTNWEIEIWGKLTAAKRSA
                     QAAWMKSIASQNAVKTALIATIAESYYTLLALDKQLAITEETINIREKSVAAIKALKE
                     SGSLSGADVVQAESNLYAAQVTIPDLKRSIRELENTLCVLTVKPLQPIARGEFDSQSM
                     PMMLHVGVPAQLLANRPDVTAAELGFRQAFEVTNVARASFYPSLRLTSGTGGISALTT
                     RTLFDPASIFVNLVGGLVQPIFQRGVLKTNLRNAKVAQQQAWNTFEKTLLTAGQEVTN
                     ALYAFETAKEKQVTRVKQIEALEKAVAFRMSLLEFSSNTNYNDVLTSEQNLISAQLTG
                     VNDKLQEFKAMIALYRSLGGGWK"
     gene            complement(1690..>3554)
                     /locus_tag="PKN22_07105"
     CDS             complement(1690..>3554)
                     /locus_tag="PKN22_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS42511.1"
                     /translation="EQGEKDPKKAAHSAMNEICGAIISITLVMAAVFIPVTFISGSAG
                     VFYKQFGITLAVAIFISAINALTLSPALCALILKPHEHEEGKKKNFVRRFFDYFNIAF
                     EKTTEKYKKSAQFFIHKKWLAFTVIAVFAGLFVLIMNSRPKAFVPNEDVGGVMSDITL
                     PPGTSVEESERVLLDIENKIKDIPGISNILRISGFSLISGSGSNYGMIIIRLKNWNER
                     KEAGQDQASIVQELFKRTASVKDAKVIFFPRPTIMGFGFSSGFEFELQDQKGGTIEEL
                     NEVNQKLLAALNARPEIQFAATSFSPNYPQYQIDLNVPAIKRAGLTVKDVLSTLQGYF
                     GGVYASDFNRFGKQYRVMYQAEPEFRANTESLNHIMVRNNKGEMAPISQFIELKKVFG
                     PQAINRFNLFTSVHIQGGPSEGYSTGDAIKAIEEVAKETLPLGYGYEFSGMSREEINA
                     GSQTIYIFLLCLVFVYFLLAAQYESYMLPLAVILSLPVGLAGAFIFAWMFDVSNNIYV
                     QITLIMLIGLLAKNAILIVEFAHEARKKGAAIFDAAIEGARSRFRPILMTSFAFIFGL
                     LPLMFAKGAGAIGNNAIGTSAIGGMLIGTLLGVFVIPGLYVVFQILHEKVSSKK"
BASE COUNT         1089 a          718 c          630 g         1117 t
ORIGIN      
        1 ttctaagcat attgaattgg aagaatccgc tcgaaagggc ggattttttt gtgagtatta
       61 ttgaaaattg gctactactg aaacctaaga tagattagga ttgtctttga atttaaggct
      121 taatagacaa aattaatgct caaaatcgtt caatccctta ctctgactaa ctataaagtc
      181 aatcaaaggt tataaaaaaa gtgcactcaa acgaatgcac tttaattatt tttaactgct
      241 attgaattac ttccaaccgc caccaagtga acggtaaagc gcaatcattg ctttgaattc
      301 ttgcaattta tcgtttacac ctgtcaattg agctgaaatt aagttttgct cagatgttaa
      361 cacatcgttg taattggtgt tgctactaaa ttctagcaaa ctcattctaa acgccactgc
      421 tttttccaat gcttcaattt gctttacacg tgttacttgt ttttctttag cagtttcaaa
      481 agcatacaaa gcattggtaa cttcttgacc tgctgttagc aatgtttttt caaacgtatt
      541 ccaagcttgt tgttgggcaa cttttgcatt tcttagattg gtttttaaaa caccacgttg
      601 aaaaataggt tgaaccaaac ctcctactaa atttacaaag atactagctg ggtcaaacaa
      661 cgtacgtgta gttaaagcac ttattcctcc tgttcccgat gtaaggcgca aagaagggta
      721 aaatgaagca cgtgctacgt tggtaacttc aaatgcttgt ctaaacccaa gttcggcagc
      781 tgtaacatcc ggacgatttg ccaataactg cgcaggaaca cctacatgaa gcatcatagg
      841 cattgattgg ctatcaaatt caccacgtgc aattggttgc aaaggtttaa ccgtcaaaac
      901 acacaacgta ttctctaact cacgaattga acgttttaaa tcgggaatgg taacttgagc
      961 agcgtataaa ttggattctg cttgcacaac atctgcaccc gacaaacttc cagattcttt
     1021 caatgctttg atggcagcaa ccgatttttc acgaatatta atggtttctt ctgtaattgc
     1081 caattgcttg tccaaagcca ataaggtata atacgattca gcaatggtgg caattaacgc
     1141 cgttttaact gcattttgcg atgcgattga tttcatccat gcagcttgag cacttctttt
     1201 tgcggctgtt aatttacccc aaatttcaat ttcccaattg gttgaaaatc caagtgaaac
     1261 agaagttgtt tgcaagttga tattgacatt ggaaggaaaa ttaagtgcat tttgtgacat
     1321 tttattatag gtcacttgcg gtgtaaaatc caagctgggc gcaaatgctt gtttggattg
     1381 tttgagcgta ttttcagcag ttgaaatttg aagaattgca ttttgcaagt catagttttt
     1441 atccaatcct tcttgaatca acgcttttaa gtgctcgtct tgaaaaaagt cttgccattt
     1501 gacatctgca agggttgtag tgtcttgcga ggtgtggtcg cgaaacaacc ctttttgttg
     1561 ctcttcagaa atggtcggtt tttcatactt ttttgtaatc accccacaac cagatagcat
     1621 accaaccgtt gcaataccaa ttactaaatt ataatatctc gatttcattg tttttacaat
     1681 tttgaattct tattttttac ttgatacttt ttcatggaga atttggaaca caacgtacaa
     1741 ccccggaatt acaaacactc ctaacaatgt accgatgagc atccctccaa ttgcacttgt
     1801 accgattgcg ttgttaccaa ttgcaccagc accttttgca aacattaaag gcaataaccc
     1861 aaaaataaat gcaaaagagg tcattaaaat tggacggaaa cgtgaacgag ctccttcaat
     1921 tgctgcatca aaaattgctg cacctttttt gcgggcttca tgggcaaatt ctacaatcag
     1981 aatggcattt ttggccaata atccgatgag cataattagt gtaatttgca cataaatgtt
     2041 attgctcaca tcaaacatcc acgcaaaaat gaatgcacca gctaatccca caggcaatga
     2101 aagaattact gccaaaggca acatgtaact ttcgtattgc gctgctaata agaagtaaac
     2161 aaataccaaa cacaataaga aaatatagat ggtttggcta cctgcattga tttcctcacg
     2221 gctcatacca gagaattcgt aaccataccc cagtggaagt gtttcttttg ccacttcttc
     2281 aattgctttg atggcatctc ctgtactata accttcactt ggaccacctt gaatatgaac
     2341 agaagtaaac aagttgaaac ggttaatggc ttgcggacca aacacttttt tcaattcgat
     2401 gaactgtgaa attggagcca tttcgccttt attgtttcta accatgatgt ggttgagtga
     2461 ttctgtattg gctctaaact caggttctgc ttgatacatt acacggtatt gtttaccaaa
     2521 acggttgaaa tcagaggcat aaaccccacc aaaataacct tgcaaggtac tcaaaacatc
     2581 tttaactgtt aatccagcac gtttgatcgc aggaacattc aaatcaattt ggtattgagg
     2641 ataattaggt gagaaggatg ttgctgcaaa ctgaatttcg ggacgcgcat tcaatgctgc
     2701 caataatttt tgattaacct cattcaattc ttcaattgtt ccaccttttt gatcttgtaa
     2761 ttcgaattca aatccactgc tgaaaccaaa tcccatgatg gttggacgag ggaagaaaat
     2821 cacttttgca tcttttacac ttgctgttcg tttaaacaac tcttgcacaa tggatgcttg
     2881 gtcttgtccc gcttctttac gttcgttcca gttttttaat cggatgataa tcattccata
     2941 gttggaacca gaaccactga ttaagctaaa tccagagata cgaagaatgt tgcttatgcc
     3001 tggaatatct ttgattttat tttcaatatc caacaacaca cgttcagatt cttcaaccga
     3061 tgtaccagga ggcaatgtaa tatccgacat cactccacca acgtcttcgt ttggaacaaa
     3121 tgcttttggt ctagaattca tgattaacac aaacaaacct gcaaaaacag caataacggt
     3181 aaaggccaac cattttttgt gaatgaaaaa ttgagccgat tttttatatt tctctgttgt
     3241 tttttcaaaa gcaatgttga aataatcaaa gaaacgacga acaaaattct ttttcttacc
     3301 ctcttcgtgt tcgtgcggtt ttaaaatcaa tgcacaaaga gctggagaca acgttagggc
     3361 gttaatagct gaaataaaaa tcgcaaccgc caaggtgata ccaaactgtt tgtagaaaac
     3421 ccctgccgag ccactgatga acgtaacagg aataaacacg gctgccataa ccaaggtaat
     3481 ggaaataatg gcaccacaaa tttcgttcat ggcgctatga gctgcttttt tagggtcttt
     3541 ttcgccttgt tcca
//
LOCUS       DAOQVQ010000217         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30226_length_3532_cov_3.012942, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000217 DAOQVQ010000000
VERSION     DAOQVQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30226_length_3532_cov_3.012942"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKN22_07110"
     CDS             complement(<1..887)
                     /locus_tag="PKN22_07110"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNS42512.1"
                     /translation="MSKKVYIVAAARTPMGSFLGSLATVTATKLGATAIEGALKKGNV
                     DPTTIDEVFMGNVLQAGVGQAPARQAALGAGISNHVPCTTVNKVCASGMKAIIMGAQS
                     ILAGDNHLVVAGGMESMSQVPHYMSARNGVKYGDAKIIDGLANDGLIDAYDRIPMGNC
                     AEVCATEHNITREDQDNFAIASYQKAAAAWQAGKFNDEVVPVSVPQRKGDPIIFAEDE
                     EYKNVKLDKIPALNPAFDKNGTITAANASTINDGASAVILASEEAVEKYGLKPIGRIV
                     SYGDAAHEPVWFTTAPAKAV"
     gene            1099..2265
                     /locus_tag="PKN22_07115"
     CDS             1099..2265
                     /locus_tag="PKN22_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNS42513.1"
                     /translation="MSDNRYNLRGVSAGKEDVHNAIKNVDKGLFPQAFCKIVPDYLSN
                     DDEYCIVMHADGAGTKSSLAYAYWKETGDLSVWKGIAQDALIMNIDDLLCVGATNNIL
                     LSSTIGRNKNLVPGEVISEIINGTEELLTELRNQGVGIHSTGGETADVGDLVRTIIVD
                     STVVCRMKRSDVISNHTIQPGDVIVGLASFGQASYETEYNGGMGSNGLTSARHDVFNK
                     SVAEKYPETFDAAVPADLVYSGSYNLTDWPGDYPLSVGKLVLSPTRTYAPIISKILEK
                     HRSNIHGMVHCSGGAQTKVLHFVGNVHIIKDNLFETPLLFRIIHEESGTDWKEMYKVF
                     NMGHRMEIYCSPEHAQEIIEISKSFNVDAQIVGRVEASDAKKLTIISEHGTFEY"
     gene            2445..3095
                     /gene="rpe"
                     /locus_tag="PKN22_07120"
     CDS             2445..3095
                     /gene="rpe"
                     /locus_tag="PKN22_07120"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNS42514.1"
                     /translation="MSQIISPSVLSCDFANIQRDVEMINNSSADWFHVDVMDGVFVPN
                     ISFGFPVVEAIKKHATKPLDVHLMIVNPDQYIETFKKAGADLLTVHYEACTHLHRTIQ
                     VIKANGMKAGVALNPHTPVSVLEDVIEELDLVLIMSVNPGFGGQKFIPHACKKVAQLK
                     KMIQEHNASTLIEVDGGVNLETGKLLLDAGADALVAGSFVFNSANPTQTIQDLKDL"
     gene            3240..>3532
                     /locus_tag="PKN22_07125"
     CDS             3240..>3532
                     /locus_tag="PKN22_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HNS42515.1"
                     /translation="MTLEQYINNINQRYKQGNATEHTFRGDLQQLLESLVSDIRATNE
                     PKRQSCGAPDYILTKKDIPVGFIEAKDIGDKDLEGAKKTGNKEQFDRYEASLN"
BASE COUNT         1089 a          638 c          687 g         1118 t
ORIGIN      
        1 acagcttttg ccggtgcagt tgtaaaccaa actggttcgt gtgcagcgtc accataagaa
       61 acaatgcgac caattggttt taatccatat ttttcaaccg cttcttctga tgctaaaatt
      121 acagcagaag caccgtcgtt tatagttgaa gcattggctg cagtgattgt tccgttttta
      181 tcaaaagcag gattcaatgc agggatttta tccaatttaa cgtttttgta ttcttcatct
      241 tcagcaaaaa tgattggatc tcctttacgt tgaggtacgc tcacaggaac aacttcatcg
      301 ttaaatttac cagcttgcca tgcagcagcc gctttttggt aagatgcaat agcaaaatta
      361 tcttggtctt cacgcgtgat gttgtgttct gttgcacaaa cttcagcaca gttccccatc
      421 gggattctgt cgtaagcatc aatcaatcca tcgtttgcca atccatcaat gattttagca
      481 tcaccatatt taacaccgtt acgtgcactc atgtagtgag gaacttgcga catgctttcc
      541 attccacctg caacaactaa atgattgtcg cctgctaaaa tagattgagc tcccatgatg
      601 atagctttca ttccagatgc acatacttta tttacggttg tacaaggaac gtggttgcta
      661 attccagctc ctaatgctgc ttgacgtgca ggcgcttgtc caacaccagc ttgtagcaca
      721 tttcccataa aaacttcgtc aattgtagta gggtcaacat ttcctttttt caatgcaccc
      781 tcaattgctg ttgctcctaa ttttgtagct gtaactgtag ctaacgaacc taagaaagaa
      841 cccataggcg tacgtgcagc tgcaacgata taaacttttt tactcatgat tttatctggt
      901 tttattattt tcaaaaacta agttcacaaa tttaagcaaa ttatcctttc aaaaaataag
      961 tactataaat ttatcaatgc cagtgcatac atttaacgga ttagaactat tttgcaatta
     1021 ttttaatttt tgttctttct aaaaatccac tctttttctc tggttttttg attacttttg
     1081 tggtcaaata gtttaaatat gtctgataat cgctacaatc ttagaggagt ttctgcaggt
     1141 aaagaagatg tgcacaatgc cataaaaaat gttgacaaag ggttgtttcc acaagctttt
     1201 tgtaaaatcg ttcctgatta tttatccaat gatgatgaat attgcatcgt tatgcatgcc
     1261 gatggtgcag ggacaaaatc ttctttggcg tatgcgtatt ggaaagaaac tggcgattta
     1321 tcggtttgga aagggattgc acaagatgca cttatcatga acattgatga tttgttgtgt
     1381 gtgggtgcaa ccaataatat tttactttct tcaactattg gacgcaacaa gaatttagtg
     1441 ccaggcgaag ttatttctga aatcattaac ggaactgagg aattgttgac cgaattgaga
     1501 aatcaagggg ttggtatcca ttccacaggt ggagaaacag ctgatgtagg tgatttggtg
     1561 cgcacgatca ttgtagattc tactgttgtt tgtagaatga aacgctcgga tgttatttcc
     1621 aatcatacca ttcaaccggg agatgtgatt gtaggattag catcatttgg tcaggcttcc
     1681 tacgaaacag aatacaatgg cggaatgggg agcaatggtt taacatctgc acgccacgat
     1741 gtgtttaaca aatcagtagc cgaaaaatac ccagaaacgt ttgatgctgc tgttcctgct
     1801 gatttagtgt attctggctc ctacaattta accgattggc caggcgatta tcctttgtct
     1861 gtaggtaaat tagtgctttc tccaacgaga acctatgcac cgattattag caaaatatta
     1921 gaaaaacacc gttcaaacat tcatggaatg gtacattgtt cgggtggagc gcaaacaaaa
     1981 gtgttgcatt ttgttggaaa tgtgcacatc attaaagata atttgtttga aacaccgttg
     2041 ttgtttagaa ttatccatga agaatcgggc accgactgga aagaaatgta caaagtgttc
     2101 aacatggggc accgtatgga aatatattgc tctccagaac atgctcaaga aattattgaa
     2161 atttcaaaat cattcaatgt ggatgcacaa attgtaggtc gagtagaagc atcagatgcc
     2221 aaaaaattga ccattatttc agaacacggg acatttgaat attaaattat acgatagcat
     2281 aattttgaat tgttaaaaat aagggcgaac aagcaactat taagtagcct acacgatttc
     2341 aattgagtaa aatagagttg aagtaagttc ttttttttaa gttcaatcct tttattgtgg
     2401 cattttctta tttccttttt atctttgcag aaaaaaatta agatatgtcg caaattattt
     2461 ctccttctgt tttatcctgt gattttgcca acattcaacg cgatgttgaa atgattaaca
     2521 actcatctgc tgattggttt catgtggatg tgatggatgg tgtttttgtg cctaacatat
     2581 cgtttggatt tcctgttgtt gaagccatta aaaaacatgc aacaaaaccg ttggatgtgc
     2641 atttgatgat tgtaaatccc gatcaataca ttgaaacgtt taaaaaagca ggagctgatt
     2701 tgttgaccgt tcattatgaa gcgtgcaccc acctacaccg caccattcaa gttatcaaag
     2761 ccaatggaat gaaagccggt gtggcactta accctcatac gcccgtgtct gtgcttgaag
     2821 atgttatcga agaattggat ttggtgttga ttatgtctgt caacccaggt tttggcggac
     2881 aaaaatttat tcctcatgcg tgtaaaaaag tagcgcaact caaaaaaatg attcaagaac
     2941 acaatgcatc tacgttaatt gaagtggatg gcggtgtcaa cttagaaaca ggtaaattgt
     3001 tgttggatgc aggtgctgat gctttagtgg caggaagttt tgtgttcaat tcagccaatc
     3061 caacacaaac tattcaggat ttgaaagatt tgtaaatcat tggtgtgcat catcgttagg
     3121 ttttgttcta atccaattgg tatagatata aaattcgaat ttgaagaact attttttata
     3181 aatagtaaca aacgaaattt tatcttaact tagttggctg taaaaagaat aacttaataa
     3241 tgaccttaga gcaatacatc aacaacatca atcaacgcta caagcaagga aatgcaaccg
     3301 aacatacttt ccgtggtgat ttacaacaat tgttagaaag tttggtttcg gacatcagag
     3361 caaccaatga acccaaacgc caatcttgtg gagctcccga ttacattctt accaaaaaag
     3421 atattcctgt tgggtttatt gaagccaaag atattggcga taaagaccta gaaggagcaa
     3481 agaaaactgg caataaagaa cagtttgacc gatatgaagc atcgctcaat aa
//
LOCUS       DAOQVQ010000218         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30243_length_3531_cov_2.817031, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000218 DAOQVQ010000000
VERSION     DAOQVQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30243_length_3531_cov_2.817031"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKN22_07130"
     CDS             <1..868
                     /locus_tag="PKN22_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HNS42516.1"
                     /translation="VNGQPYKKRYIAHWDLVGAKTLEFVMGPFPSSEYRTYEVAPSID
                     SIPTSFVPVPYFTNTTSMFEKSTKVAIASPMKDLKYYYTLDGSTPTLKSKKYTKPFSI
                     KKTTTVKVFAYNPKTAQKSQVITNEFRIKPSDLSLQLNSEYAKQYSASGNNALIDGLY
                     GSNDFRSGDWQGFYNNNVNGVVTFSKTKNLKTIGLSALQGTRSWIFPPKAVEFKITYV
                     DGEVETKMVDLEKHPSGDWSPEQPVKFEVTPKNKAIQSVEFTAYNYGVNPTWHLSPGY
                     PTFIFLDEIYFK"
     gene            999..1072
                     /locus_tag="PKN22_07135"
     tRNA            999..1072
                     /locus_tag="PKN22_07135"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1033..1035,aa:Ile,seq:cat)
     gene            complement(1235..1311)
                     /locus_tag="PKN22_07140"
     tRNA            complement(1235..1311)
                     /locus_tag="PKN22_07140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1275..1277),aa:His,seq:gtg)
     regulatory      1492..1676
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1793..2764
                     /locus_tag="PKN22_07145"
     CDS             1793..2764
                     /locus_tag="PKN22_07145"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HNS42517.1"
                     /translation="MKKVYFFAVALLATSTVFAQNDTLTFEQYDLGSNTYYNGADGAG
                     NITIGNYTLSNNYNTQYGYMENGFAISKVQDITTAGFTNQYASYANGGANNSEKYGVC
                     YSGEITFSSLRKIKSLMVTNTTYAGLSMRDGDSFGKQFGSPNNAAGTPDGTNGEDWFL
                     LQIIPLDENNQVVGDTVDFYLADFRFADNNQDYILNTWETVQMNDVLAKKLTFKLSSS
                     DVGQYGMNTPAYFALDNLVTAPNDASVKSVANAAVSIYPNPANDVLNIVTNEATSIEV
                     LNAVGKVVKTASVNGKTTLNVSELPTGVYYVSMNSTAGKSIQKVVIK"
     gene            2772..>3531
                     /locus_tag="PKN22_07150"
     CDS             2772..>3531
                     /locus_tag="PKN22_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42518.1"
                     /translation="MKFYLVVVICWLMAFPTLSQKEKEKEETQLQEINVIATKLNKYF
                     VDSSQVYISRHEIEALQPDDLGELLKKLPGVNVKSYGGLGGFKTVSIRGLSGQHTSFV
                     VDGFSQTNAQTGQINMGQIQLDNIESVVVQRGGASELIVPAIAQLSGNAIVLETFQSQ
                     SPTIPFQQKLMAKFGAFGQNDYNYIGKTGSKKLFGGIYFKYRNTHGEYPFKYMNYKTQ
                     LTGVRKNNDYSDINGGFNLSYQPVDNHKLNFHIQY"
BASE COUNT         1201 a          636 c          632 g         1062 t
ORIGIN      
        1 ggtaaatgga caaccttata aaaaacgtta cattgcgcat tgggatttgg ttggtgctaa
       61 aacgttggag tttgttatgg ggccatttcc atcctctgaa tacagaacct atgaagtagc
      121 tccgagtata gattctattc caacttcatt tgtgccagtt ccctatttta caaacacaac
      181 ttccatgttt gaaaaatcta ctaaagtggc cattgcaagt ccgatgaaag atttgaaata
      241 ttactacacg ttggatggtt ctacaccgac attaaaatca aaaaaataca caaaaccatt
      301 ttctattaaa aaaacaacaa cggtaaaagt gtttgcatac aatccaaaaa cagcacaaaa
      361 aagccaagtt attaccaatg aatttagaat taaaccaagc gatttaagtt tgcaattaaa
      421 ttctgaatac gccaaacaat attcagcttc gggcaacaat gcgttgattg atggtttgta
      481 tggttctaac gattttagaa gtggcgattg gcaaggattt tacaacaaca atgtgaatgg
      541 agttgtaaca tttagcaaaa caaaaaattt aaaaacaata ggtttatcgg cacttcaagg
      601 tacgcgttca tggattttcc ctccaaaagc agttgaattt aaaatcacgt atgtggatgg
      661 tgaggttgaa actaaaatgg ttgatttaga aaaacatcct tctggagatt ggagcccaga
      721 gcaacctgtt aaatttgagg ttacaccaaa aaataaagca atccaatcgg ttgaatttac
      781 agcctacaat tatggtgtaa atccaacgtg gcatttaagt ccgggctacc ctacttttat
      841 ctttttggat gaaatttatt ttaagtaaaa tgcaagcaaa ctctaattaa tctggcaact
      901 ttttttctga taatgttgct attttaaatg gatgtattat atttgccagc cgaatggaat
      961 aagcgattcc aatagcaaaa cgaaaatatg attttcgagg tcctatagct cagttggtta
     1021 gagcacctga ctcataatca ggtggtcgct ggttcgagcc cagctgggac cacaggtaaa
     1081 gagtaagatt aatttcttac tctttttttt ttgagttttt ttggcgagaa gtttatgccg
     1141 tcgtaatttt caaggaggta cgacgtagaa aataagaacg gtaagcccag ctgggaccat
     1201 aaaaaaagag gctgcctata aagacaacct cttttggggt ggaagatcgg tctcgaaccg
     1261 acgaccttcg gaaccacaat ccgacgctct aaccaactga gctacaacca ccataaaaca
     1321 actgtaaaac caattgcgag tgcaaatata gctaaacttt agaaaaaaaa tacacctaca
     1381 tcaaaaaatt tcaacttttt ttttcgatta atcatcacaa agcaaccatt aacattgaat
     1441 tttaatcgtt tagctaaatc actctcgtat ttaacattta aaatcgacaa ctatctccta
     1501 ttaatttatt atctttgcaa cgcttttagt aaaggaattt ggtgaaaatc caaaactgta
     1561 actgcagctg taagtgctgt gaaacaattt caacaaaagc cactgttcat actttgaatg
     1621 ggaaggcgaa attggttagc acaagtcaga atactatact atatagctta atttaacgaa
     1681 ggcttcagat taaagtcggg cgttactcaa taactcgctt ccgttattgt ttgatttcct
     1741 ccatttaact gattcttcac ttttatttat taacttttta aaatttaaaa gcatgaagaa
     1801 agtttatttt ttcgcagttg ctcttttggc aacatctaca gttttcgcgc aaaacgacac
     1861 acttacattt gaacagtatg atttaggttc aaacacgtat tacaatggtg ccgatggtgc
     1921 aggtaatatt accattggta actacacatt gtccaataac tacaacaccc aatatggtta
     1981 tatggaaaat gggtttgcta tttcaaaagt acaagacatc acaactgctg gtttcaccaa
     2041 ccaatacgct tcttacgcta atggaggagc aaataactct gaaaaatacg gtgtatgtta
     2101 cagtggtgaa attacttttt catcacttcg taaaatcaaa agcttgatgg taaccaacac
     2161 tacctacgct ggtttatcaa tgcgcgatgg ggatagcttt ggaaaacaat ttggttcacc
     2221 aaacaatgct gctggaactc cagatggtac caatggcgaa gactggtttt tattgcaaat
     2281 catccctctt gatgaaaaca atcaagtagt tggagatacc gttgattttt atttagcaga
     2341 tttccgtttt gctgacaaca accaagatta catcctaaac acttgggaaa cagtgcaaat
     2401 gaacgatgtg cttgctaaaa aattgacttt caaattgtct tcttctgacg ttggtcaata
     2461 tggtatgaat actccagctt attttgcatt ggataatttg gttacagcac caaacgatgc
     2521 atctgttaag tcagtagcta atgcggcagt tagtatttat cctaatccag ctaacgatgt
     2581 attaaacatt gttaccaacg aagctacttc aattgaagtg ttaaatgcgg ttggaaaagt
     2641 ggttaaaact gcatctgtta acggtaaaac tacattgaat gtaagtgaac ttccaacagg
     2701 tgtttactat gtttcaatga attcaactgc tggaaaatca attcaaaaag ttgtaattaa
     2761 ataagattca aatgaaattc tatttggtag tagtgatttg ttggttgatg gccttcccta
     2821 ctctttctca aaaggaaaaa gaaaaggaag aaacacaatt gcaagaaatc aacgtcattg
     2881 ctaccaaatt gaacaaatat tttgttgact catcgcaagt gtatatttcg cgccatgaaa
     2941 ttgaagcttt gcaacccgat gacttgggcg aattattgaa aaaattgccc ggtgtaaacg
     3001 ttaaatcgta tggtggatta ggcggattta aaaccgtttc catcagagga ttgagcggac
     3061 aacataccag ttttgttgta gatggttttt cgcaaaccaa tgcacaaacg ggacaaatca
     3121 acatggggca aattcaatta gataatattg aatctgtggt agtgcaacgt ggtggagcat
     3181 ccgaattaat tgttcctgca attgcacaat taagtggaaa tgcaattgta cttgaaacct
     3241 ttcaatccca atctccaacc atcccttttc aacaaaaatt aatggccaaa tttggcgctt
     3301 ttggacaaaa cgattacaac tatatcggaa aaacaggaag caaaaaacta tttggaggta
     3361 tttactttaa atacagaaac acacacggtg aatatccttt caaatacatg aattacaaaa
     3421 cgcaattaac tggcgttcgt aaaaacaatg attactcaga catcaatggt gggttcaatc
     3481 tgtcttacca accagttgat aatcataaac ttaattttca cattcagtac c
//
LOCUS       DAOQVQ010000219         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30312_length_3525_cov_3.879827, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000219 DAOQVQ010000000
VERSION     DAOQVQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30312_length_3525_cov_3.879827"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..439)
                     /gene="rplP"
                     /locus_tag="PKN22_07155"
     CDS             complement(11..439)
                     /gene="rplP"
                     /locus_tag="PKN22_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNS42519.1"
                     /translation="MLQPKRTKFRKAQKGRNRGMAHRGSTIAFGSFALKTLEPAWITS
                     RQIEAARIALNRYMKREGKVWIRIFPDKPVTSKPAEVRMGKGKGAPSHWVAVIKPGCI
                     IFEADGVPYEIAKEAMRLASQKLPVKCKFIVRNDFVIPEN"
     gene            complement(463..1197)
                     /gene="rpsC"
                     /locus_tag="PKN22_07160"
     CDS             complement(463..1197)
                     /gene="rpsC"
                     /locus_tag="PKN22_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNS42520.1"
                     /translation="MGQKTNPIGNRLGFIHGWESNWYGGDNYKDKLVEDDKIRKYLQA
                     RLSRASIAKIIIERTLKLVTVTINTARPGVIIGKGGQEVDKLKEELKKITKKEIQINI
                     FEVKRPELDARLVADGIARQLEARISFRRAIKMAIASTMRMGAEGIKVKISGRLNGAE
                     MARSEMYKDGRTPLHTFRADIDYAISEAHTTYGRLGIKVWICKGEVFGKRDLSPNVGQ
                     AQSAGKASAGGAKKKFAAGGAKKRKK"
     gene            complement(1207..1602)
                     /gene="rplV"
                     /locus_tag="PKN22_07165"
     CDS             complement(1207..1602)
                     /gene="rplV"
                     /locus_tag="PKN22_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNS42521.1"
                     /translation="MGARKQLRAKQIKEEKASTAFARLNDSPIAPRKMRLVADLVRGV
                     EVNKALNILQHNPKAASVSLEKLLRSAIANWEQKNEGKSIEDEKLVVRSIQVDGGAMM
                     KRIQAAPQGRAHRIRKRSNHVTIIVDGVK"
     gene            complement(1626..1904)
                     /gene="rpsS"
                     /locus_tag="PKN22_07170"
     CDS             complement(1626..1904)
                     /gene="rpsS"
                     /locus_tag="PKN22_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNS42522.1"
                     /translation="MSRSLKKPPFVHYKLMMRVDDAQASGKKDVIKTWSRASTITPDF
                     VGLTFAVHNGNKFIPVFVTENMVGHKLGEFSPTRSFRGHGGNKKDKKR"
     gene            complement(1912..2736)
                     /gene="rplB"
                     /locus_tag="PKN22_07175"
     CDS             complement(1912..2736)
                     /gene="rplB"
                     /locus_tag="PKN22_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNS42523.1"
                     /translation="MSLKKFKPTTPGQRHKINSDYSEVTAAKPEKSLVVGKSKTGGRN
                     HDGKMTMRYIGGGHKQKYRIIDFKRSKQGIPATVKSIEYDPNRTARIALLYYADGEKS
                     YIIAPEGLKVGDTVMSGKGATPNVGNALFLSDIPLGTVIHNIELTPGKGGAIARGAGA
                     YAQLNARDGRYAIVKMPSGETRLILTTCLATIGSVSNSEHSLVVSGKAGRKRWLGRRP
                     RVRGVVMNPVDHPMGGGEGRNAGQHPRSRKGTPAKGYKTRAPKKYSDKLIIERRKK"
     gene            complement(2750..3046)
                     /gene="rplW"
                     /locus_tag="PKN22_07180"
     CDS             complement(2750..3046)
                     /gene="rplW"
                     /locus_tag="PKN22_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNS42524.1"
                     /translation="MKTVIKKPIITEKATGQSEKQNRFSFVVDREANKLEIKSAIEKL
                     YGVQVTGVHTMNYGGGKSSVKYTNKGIVEQRSKQWKKAVVTVADGETIDLFSNL"
     gene            complement(3054..3296)
                     /gene="rplD"
                     /locus_tag="PKN22_07185"
     CDS             complement(3054..3296)
                     /gene="rplD"
                     /locus_tag="PKN22_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNS42525.1"
                     /translation="MNFNNAKTKDFTALLNGLNLNDQKVLFILGSDNENVYKSSRNLR
                     KVKVVTADSFNTYDVLNAKKVVLATSSIEAIEKILN"
     gene            3283..>3525
                     /locus_tag="PKN22_07190"
     CDS             3283..>3525
                     /locus_tag="PKN22_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42526.1"
                     /translation="MLKFTSSITINDQEVAYYWRAVHKDNGKISPNQGQIVGFNLIQD
                     GFILGRSNPYSTNDDKSYEPIKLVQNYLNWYIGEIHI"
BASE COUNT          969 a          749 c          550 g         1257 t
ORIGIN      
        1 atactaaagg ctaattttct ggaattacaa aatcattacg aacgataaac ttacatttaa
       61 ctggaagttt ttgagatgcc aaacgcatag cttcttttgc tatttcgtaa ggaacaccgt
      121 ccgcttcaaa aataatacat ccgggtttaa taactgcaac ccagtggctt ggagcacctt
      181 tccctttacc catacgtact tcagctggct tagacgtaac tggtttatct gggaatatac
      241 gaatccatac ttttccttca cgcttcatgt aacggttaag cgcgatacga gcagcttcga
      301 tttggcgtga agtaatccac gctggctcca aagtttttaa tgcaaatgat ccgaaagcaa
      361 tcgtactacc tctatgggcc ataccacggt ttcttccctt ttgcgctttt ctaaatttgg
      421 ttctttttgg ctgtaacatt tttctaaaac tttactgttt tattactttt ttcttttctt
      481 agcaccacct gctgcgaatt tcttcttagc accacctgca cttgctttac cagcagattg
      541 agcttgacca acatttggtg ataaatcacg ttttccgaat acttcaccct tacatatcca
      601 cactttgata cctaagcgac cataagtcgt gtgagcttca gaaattgcat aatcgatgtc
      661 agctctgaac gtatgtaacg gagtacgtcc atctttgtac atctctgaac gagccatctc
      721 tgcaccattc aaacgtccag aaatcttaac tttgattcct tctgctccca tgcgcattgt
      781 actcgcgata gccattttaa ttgctcttct gaaagaaatt ctcgcttcta attgacgagc
      841 aataccatct gcaactaaac gagcatccaa ttcaggtctt tttacttcaa agatgttgat
      901 ttggatttct tttttagtta tcttttttaa ctcttctttt aacttgtcaa cctcttgtcc
      961 acctttaccg atgatgacac ctggtcttgc agtgttaatt gtaactgtga caagtttcaa
     1021 ggtacgttcg atgataattt tagcgataga agctcttgaa agacgtgctt gtaaatattt
     1081 acgaatttta tcgtcttcaa ctaacttatc tttgtagtta tcaccaccat accagttaga
     1141 ttcccatcca tggatgaaac ctaaacgatt acctattgga tttgtttttt gtcccatttt
     1201 caaaaattat ttaacaccat caacgataat ggtaacatgg tttgatcttt ttctaattct
     1261 atgcgcacgt ccttgtggag cagcttgaat tcttttcatc attgcaccac cgtctacttg
     1321 aatagagcga actactaatt tttcgtcctc aatactttta ccttcatttt tttgttccca
     1381 attggcaatt gcagaacgta aaagtttctc taagctaaca gacgcagctt taggattgtg
     1441 ttgcaaaata tttagagcct tatttacttc tactcccctt acaagatctg caactaatct
     1501 catctttctt ggagcaatcg gagaatcatt caaacgtgcg aatgcagtcg aagctttctc
     1561 ttctttaatt tgctttgctc ttaattgttt tctagcaccc ataatttgct aataacttat
     1621 taatactatc ttttcttatc ttttttattt cccccgtgtc cacggaaact acgagttggt
     1681 gaaaactcac ctaacttatg tccaaccatg ttctcagtta cgaaaactgg aatgaatttg
     1741 tttccattgt gtacagcgaa tgttaatcca acaaaatcag gagtaattgt acttgctctt
     1801 gaccaagttt tgataacatc tttctttcct gatgcttgtg catcgtccac tctcatcatt
     1861 aatttataat gaacaaacgg tggttttttc aacgaacgac tcatacctta cttatttttt
     1921 tcttctttcg atgatcaact tatctgaata tttcttaggc gcacgagttt tgtatccttt
     1981 agctggagta cctttacgtg agcgtgggtg ttgtcctgcg ttacgacctt caccaccacc
     2041 cattgggtga tctactgggt tcataactac accacgtaca cgtggacgac gacctaacca
     2101 acgttttcta cctgctttac ctgaaacaac taagctgtgt tcagagttag aaacagaacc
     2161 aatagttgcc aaacaagttg ttaaaatcaa tctagtttca ccagaaggca ttttaacgat
     2221 tgcataacga ccatcacgag cgttcaattg agcgtatgca ccagctccac gagcgatagc
     2281 accaccttta ccaggtgtta attcaatgtt atgaattaca gtacctaatg gaatatcgct
     2341 taagaataaa gcatttccta cattaggtgt agctccttta ccagacatta cagtatcccc
     2401 tactttcaat ccttccggag caataatata tgacttttca ccgtcagcgt aatacaataa
     2461 ggcaatacgt gcagtacggt ttggatcgta ttcaattgat ttaaccgttg ctggaatacc
     2521 ttgtttactt cttttaaaat cgatgatacg gtatttttgt ttgtgaccac caccaatgta
     2581 gcgcatcgtc attttaccgt catgattacg tccaccagtt ttactctttc caactaccaa
     2641 agatttttct ggctttgcag ctgttacttc agagtaatca ctgttgattt tgtgtctttg
     2701 ccctggagtt gtaggcttaa attttttaag actcatttcg ttttacttct tataaattac
     2761 taaataaatc aatcgtttct ccatctgcaa cagttacaac agcttttttc cattgtttgc
     2821 tgcgttgctc aacgatacct ttgttagtgt acttaacaga ggatttacca ccaccataat
     2881 tcattgtatg tactcctgtt acttgaactc catacaattt ctcgatagca cttttaattt
     2941 ccaatttatt tgcctctctg tcaacaacaa atgaaaaacg gttttgtttt tcgctttgtc
     3001 ctgttgcctt ttctgtaata attggctttt taataactgt cttcatcttt aaatcaattt
     3061 agaatctttt cgattgcttc tattgaactt gttgccaaaa caactttttt agcatttaat
     3121 acatcgtatg tattgaatga atcagctgtt acaactttaa cttttctaag gttacgagaa
     3181 gatttgtaaa cattctcatt atcacttcct aaaatgaaca atactttttg atcgttcaag
     3241 tttaaaccgt ttaataatgc tgtaaaatct tttgtttttg cattgttaaa gttcacatca
     3301 tcaataacga ttaatgacca agaggttgca tattattggc gagcagttca taaagataat
     3361 gggaaaattt ctccaaatca agggcagatt gtgggtttta atttgataca agatggattt
     3421 attttaggtc gatcaaatcc atattccaca aatgatgata agagctatga acctattaag
     3481 ttagttcaaa attatttgaa ttggtatatt ggcgaaatcc acatt
//
LOCUS       DAOQVQ010000220         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30466_length_3514_cov_3.228101, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000220 DAOQVQ010000000
VERSION     DAOQVQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30466_length_3514_cov_3.228101"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKN22_07195"
     CDS             complement(<1..803)
                     /locus_tag="PKN22_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNS42527.1"
                     /translation="MKPFGTKNIVVLGAGESGVGAAVLAKKQGWNVFVSDFGTIKDTF
                     KEELNSYQLEWEEGKHTELRVLDAQIVVKSPGIPDKAPIIKKLKEKNIPVISEIEFAG
                     YYNTAKTICITGTNGKTTTTMLTYHILKKAGIKVGLAGNVGKSFAKQVAEEQFDWYVL
                     ELSSFQLDGMFEFKADVAILLNITPDHLDRYDYQLQNYVDSKFRILQNMGPNDWFIYN
                     ADDAILNQEVEKRTITANLAPFSLTKELLQGGFIKDKQLIDDIVEYINS"
     gene            complement(803..2047)
                     /gene="mraY"
                     /locus_tag="PKN22_07200"
     CDS             complement(803..2047)
                     /gene="mraY"
                     /locus_tag="PKN22_07200"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNS42528.1"
                     /translation="MLYYIFNYLDQINVPGAGVFHYISFRAAMALITSLVISIIIGKR
                     IINVLRKQQIGETVRDLGLQGQLEKSGTPTMGGIIILSAIFIPTLLFAKLDNIYIITM
                     LLSTIWLGLIGFLDDYIKVFKKNKEGLKGKFKIVGQIGVGILVGSMLYFSDDVKVHKK
                     VPADYVLQSDEHIVSHMHDNSEPNTKWIESDHITTSIPFVKGNEFNYNWLIGENGTSP
                     YGWLIFIPVVIFIVTAVSNGANMTDGLDGLATGTSAIIGIALAVLAYVSSHVTFADYL
                     NVMYIPRAEEMVIFISAFVGACIGFLWYNSFPAQVFMGDTGSLALGGIIAVFAIAIRK
                     ELLIPVLCGIFLIENLSVMMQVGYFKYTKKRFGEGRRIFKMAPLHHHYQKSGLHEAKI
                     VSRFWIVAVLLAVISIVTLKMR"
     gene            complement(2062..3096)
                     /locus_tag="PKN22_07205"
     CDS             complement(2062..3096)
                     /locus_tag="PKN22_07205"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNS42529.1"
                     /translation="MLDITQHSDYHKLSEQIFEKIKIAQSKALFKVNAEMLTLYWEIG
                     SLIISNQNQHGWGSKIIEFLSKDLAKSFPNNSGFSIRNLKYMKAFAEAYPEFPIVQVG
                     LAQKNEGKVQVELAQISWYHHISLLTKIKDVNKRAFYIVETAKNEWSRNIMLLQIQNN
                     YYERKGKATNNFDKTLPSHQSELAKEVFKNPYNFEFLMLSERVREYEIEKLLTEKITS
                     FLLELGKGFAFVGRQYPITIDNTDYKIDLLFYHTILHAYVVVELKAGEFMPEYVSKLN
                     FYTNAIDDKLKTNKDENTIGLLLCASKSDVKVEYAMRGMEKPLGVATYQLAKLVKENM
                     KQFITLNNEE"
     gene            complement(3096..>3514)
                     /locus_tag="PKN22_07210"
     CDS             complement(3096..>3514)
                     /locus_tag="PKN22_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNS42530.1"
                     /translation="PDALENVLKTITNIRTKNETLFTIIGCGGDRDKAKRPEMAAIAC
                     ELSDKVIFTSDNPRTEDPEQILDDMSKGVPGHHFKKTLTVVDRKQAIKTAISMAQKGD
                     IILIAGKGHEKYQEINGVKHDFDDRKIAMELFEKMN"
BASE COUNT         1028 a          679 c          589 g         1218 t
ORIGIN      
        1 ttactattga tatattcaac aatatcatca attaattgtt tatctttaat aaaacctcct
       61 tgcagaagtt cttttgtcag tgagaatggc gccaaattgg ctgtgatggt gcgtttttca
      121 acctcttgat taagaatagc atcatcagca ttgtaaataa accaatcgtt gggtcccatg
      181 ttttgaagaa tgcgaaactt actgtccaca tagttttgca attggtagtc gtatctatcc
      241 aaatggtcgg gagtgatgtt gagcaaaata gcaacatcgg ctttgaactc aaacataccg
      301 tccaattgaa aagaacttaa ttccaacaca taccaatcaa attgttcttc ggcaacttgt
      361 ttggcaaagc ttttacccac gtttcctgct aatcctactt tgataccggc ttttttcaaa
      421 atgtggtagg ttagcatggt ggtggttgtt tttccatttg tacccgtgat acagatggtt
      481 tttgcggtat tgtaatatcc tgcaaattca atttctgaaa taacaggaat gtttttttct
      541 ttgagttttt taatgatggg tgctttgtcg ggaataccag gtgatttcac aactatttga
      601 gcatccagta cgcgcaattc ggtgtgtttt ccttcttccc actccaattg atagctgttt
      661 aactcttctt taaaagtatc cttgattgtt ccaaaatccg aaacaaaaac attccaccct
      721 tgcttttttg caagtacagc agcacctacg ccagattcac cagcgcccaa cacaacaata
      781 ttttttgttc caaagggttt cattatctca ttttaagcgt tacaatggag attacagcaa
      841 gtaaaaccgc tacaatccaa aagcgggaaa caattttagc ttcgtgcaat cccgattttt
      901 gatagtgatg atgcaaaggc gccattttaa agatgcgtcg tccttcaccg aatcgttttt
      961 tggtgtattt gaaatatcct acctgcatca ttaccgataa attttcaatt aagaaaatgc
     1021 cacacaatac tggaatcaac aattctttgc gaatggcaat ggcaaacaca gcaatgattc
     1081 ctcccaatgc taaacttcct gtatcgccca taaatacttg agcagggaag gagttgtacc
     1141 acaaaaatcc aatacatgcg cccacaaatg ccgagatgaa aattaccatt tcctccgctc
     1201 gtggaatgta catcacattg aggtaatcgg caaaggttac gtgcgagctt acataagcca
     1261 aaacagcaag agcaatccca ataattgccg atgttccggt agccaatccg tccaaaccat
     1321 cggtcatgtt agcaccattt gaaacggctg ttacaatgaa aataactact ggaataaaaa
     1381 tcaaccaacc ataaggcgat gttccgtttt cgccaatcaa ccagttgtag ttgaattcat
     1441 tgccctttac aaatgggatg gaagttgtta tgtgatccga ttctatccat ttggtgttgg
     1501 gttcactgtt atcatgcatg tgcgaaacaa tgtgttcgtc ggattgcaaa acatagtctg
     1561 caggcacttt tttatgcact ttcacatcgt ctgagaagta aagcatggaa cctaccaaaa
     1621 tgcccacacc aatttgacca acgattttaa attttccttt taatccttct ttgttctttt
     1681 taaaaacttt gatgtaatcg tccaaaaagc caatcaatcc caaccaaatt gtagataaca
     1741 acatggtgat aatgtagatg ttatccaatt ttgcaaacaa caacgttgga ataaaaatgg
     1801 cactcaaaat gatgatccca cccatggttg gcgtacccga tttttccaat tgtccttgca
     1861 aacctaggtc gcgcacagtt tctccaattt gttgtttgcg caacacgttg atgatgcgtt
     1921 ttccgatgat aattgaaata accaacgagg taatcaatgc cattgctgca cggaacgaaa
     1981 tgtagtgaaa cacacctgct cccggcacat tgatttgatc taagtaatta aaaatatagt
     2041 acaacatgtg tttttgttta tttattcttc gttatttaat gttatgaatt gcttcatatt
     2101 ttcctttaca agttttgcaa gttgataggt agcaacgcca agaggtttct ccattcctct
     2161 catagcatac tctactttaa catcgctttt tgatgcacat aaaagcaatc caattgtgtt
     2221 ttcatcttta tttgttttta gtttgtcatc tattgcattt gtgtagaagt ttaattttga
     2281 tacatattca ggcatgaatt cacctgcttt taactctaca acaacatatg cgtgcaagat
     2341 ggtgtgataa aacaataaat ctattttata atcagtatta tctattgtaa tagggtattg
     2401 tcttcctacg aatgcaaatc cttttccgag ttctaaaaga aatgaagtaa ttttttctgt
     2461 caaaagtttt tcaatttcat attctctaac cctctcagaa agcattaaaa attcaaaatt
     2521 gtatggattt ttaaatactt ctttagccaa ttctgattga tgagaaggta atgttttatc
     2581 aaaattattg gttgccttcc cctttcgctc gtaatagttg ttttgtattt gaagtagcat
     2641 aatatttcta ctccattcat ttttagctgt ctcaactata taaaaagctc ttttgtttac
     2701 atcttttatt tttgttagta agctaatgtg gtggtaccaa gaaatttgtg caagttccac
     2761 ttgcactttt ccctcattct tttgtgcaag tcccacttgc acaattggga attctgggta
     2821 agcttcagca aaagctttca tatattttaa gttgcgaata gaaaatccag aattatttgg
     2881 aaatgatttt gctaaatctt ttgatagaaa ttctattatt ttactacccc aaccatgttg
     2941 attttgattt gaaataatta acgaacctat ctcccaataa agcgtaagca tctctgcatt
     3001 aactttaaat aatgcttttg actgagcaat ttttattttt tcaaaaattt gctcagataa
     3061 tttatgataa tcgctatgtt gagtgatatc taacattaat tcattttttc aaataattcc
     3121 attgcaattt tacggtcgtc aaagtcgtgt tttacaccgt ttatttcttg gtatttttcg
     3181 tgtccttttc cggcaattaa gatgatatcg cctttttgag ccatagaaat tgcagtttta
     3241 attgcttgtt tacggtcaac aacggtcaat gtttttttga aatgatgacc aggcacacct
     3301 ttgctcatat cgtctaaaat ttgttctgga tcttcggtac gaggattgtc ggatgtaaaa
     3361 atgactttgt cgctcaattc acatgcaatt gcagccattt ctggacgttt tgctttatct
     3421 ctatcgccac cacaaccaat gatggtaaac aaagtttcgt tttttgtgcg gatgttggta
     3481 atggttttta atacattttc caaggcatcg ggtg
//
LOCUS       DAOQVQ010000221         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30505_length_3511_cov_3.515046, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000221 DAOQVQ010000000
VERSION     DAOQVQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30505_length_3511_cov_3.515046"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKN22_07215"
     CDS             complement(<1..561)
                     /locus_tag="PKN22_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNS42531.1"
                     /translation="MPSESKEVHRKVLPKVKNIIAVASGKGGVGKSTITANIAGGLLK
                     AGYTVDIVDADIYGPSMPTMFDVVNMRPKMLTIDGVPKIDPVETHGVKLLSIGFFTEQ
                     DNAVVWRGPMAAKALTQMFTDAEWGELDYLLIDLPPGTGDIHLSLVQNVPLDGAVIVS
                     TPQEVALADARKGINMFKLDTINVPVI"
     gene            complement(625..801)
                     /locus_tag="PKN22_07220"
     CDS             complement(625..801)
                     /locus_tag="PKN22_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNS42532.1"
                     /translation="MELTRENVLEVLGRIQDPELKNDIVSLSFVEELKIEDNTVSVKV
                     LSSNPALHARKRLH"
     gene            complement(1057..1896)
                     /locus_tag="PKN22_07225"
     CDS             complement(1057..1896)
                     /locus_tag="PKN22_07225"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNS42533.1"
                     /translation="MNFKIKRQTGEVSEFDVEKLKYSLTKSGASDIEVAEVLENMLPR
                     IHDGMSTKAIYQLAFNQLKKVNGTFAARYSLKRALMELGPAGFYFEQWIARFLESYGY
                     ETCTNVLVQGEAVTHEADVIAKKGEEMLWIECKFRNTMDSKISVTTPMYVLSRVNDIS
                     QQTYPFFGGAHRFTHGWLITNAYFTSDAIAFGEHYGLKMLSWDYPAKRSIKNLVDQKA
                     LYPITCLTSLTKNEKDYLLSKGFILVKDLYGNDEVFESNFIKGKNRQKVKFEIEELIQ
                     GNE"
     gene            complement(1903..2775)
                     /locus_tag="PKN22_07230"
     CDS             complement(1903..2775)
                     /locus_tag="PKN22_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42534.1"
                     /translation="MKFALNFLVFLFVCNTITYSQSFKEKVESIYNFKIATLTPDEAI
                     KKNEELRLFWKEMDKDTANFLPQIRKVLTIPNQPSYLYFDLCSYLNIQSFKPSDNALI
                     EQAIANINWADMNQWELIEKYRTFALSGINVVNGVKTLFKTTKINVLNPLINKTFNQG
                     EIAAYLLLPLKPQKYVTPLAEIYDTISTEAQRTVITILWLSNTQVGDEKLQLFAKTTK
                     DIDVRSYANRLLYRFPPSQELIVPLEAMNEQQRTADLMQNYFQSVAEWKADSWQKIIQ
                     TTQLMHYFKLARSL"
     gene            2958..>3511
                     /locus_tag="PKN22_07235"
     CDS             2958..>3511
                     /locus_tag="PKN22_07235"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687877.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNS42535.1"
                     /translation="MNNYKHQVRENFIRYAKIDTTADPNSTTFPSSMKQKDLAQLLVK
                     ELQDMGASEVEMDEWGYVFATIPSNTDKVTSTICFCSHMDTAPDVTGTNVKPIVHEKY
                     NGQPIVLPDDPTQIITTEMHPYLKEKIGDDIITASGTTLLGADDKAGVAIIMDFANYL
                     LTHPEVKHGKVRILFTPDEEVGRGV"
BASE COUNT         1140 a          671 c          537 g         1163 t
ORIGIN      
        1 tataactgga acgttaatag tgtctaattt aaacatattg attccttttc ttgcatcggc
       61 taatgccact tcttgaggag ttgaaacaat aacagctcca tccaaaggta cattttgcac
      121 taaagataag tgaatatcac ctgtacctgg aggaaggtca atcaataaat aatccaactc
      181 accccactct gcatctgtaa acatttgcgt caaagctttt gcagccattg gtccacgcca
      241 aacaaccgca ttatcttgct ctgtaaaaaa tccaattgac aataatttaa caccatgcgt
      301 ttctacaggg tcaatttttg gaacaccatc aatggttaac attttaggtc gcatattaac
      361 aacgtcgaac attgtaggca tagaaggtcc ataaatatcc gcatcgacaa tatcaactgt
      421 atagccagct tttaacaaac cacctgctat attggctgta attgttgatt ttcccacacc
      481 accttttcca gaagcaactg caataatatt tttaactttt ggcaatactt tacgatgcac
      541 ttctttcgat tctgatggca aaggaatagt tgaacattta acaacaatgt ttggaaactc
      601 tctttttaag ttaaactcaa cggcttagtg caagcgtttg cgcgcatgca atgcaggatt
      661 agagcttaat actttaacag aaacagtatt atcttcaatt tttaactcct ctacaaaact
      721 taaagaaaca atatcgtttt tcaattctgg atcttgaatt ctacctaaca cctccaagac
      781 attctctctt gtcaactcca tatataaata attttgctgt gtaaaattac atattttttc
      841 tttaaactat ttgtattaat tctaaataaa gtaaaaaaga atccacaata atctgttaac
      901 ttgcttaaaa tcggataacc gactactttt tattttgcga gaagccttca atatactaat
      961 ttcaaacaaa tgaaataact ataaatttat aagatgaagc taaaaacaac taatgattaa
     1021 acatttcaaa caaagagatt tattgggtgc actaagctat tcgtttcctt gaattaattc
     1081 ttctatttca aatttcacct tttgacggtt tttccctttg ataaaattgc tttcaaaaac
     1141 ttcatcgttg ccatacaagt cctttaccaa aataaatcct ttgctgagca aataatcttt
     1201 ttcatttttg gttaatgagg ttaaacaagt aatgggatac agcgcttttt gatcaaccaa
     1261 gttcttaata cttctttttg caggataatc ccacgatagc atttttaagc catagtgctc
     1321 gccaaaagca atggcatctg aggtaaaata ggcatttgtt attaaccaac catgtgtaaa
     1381 acgatgagct ccaccaaaaa atggatacgt ttgctgtgaa atatcgttca cgcgcgataa
     1441 aacatacata ggtgtggtaa cgctgatttt agaatccatg gtatttctaa acttgcattc
     1501 tatccataac atctcctcgc cttttttggc aatcacatct gcttcatgcg tcacagcctc
     1561 tccttgtact aatacatttg tacatgtttc atagccatag ctttctaaaa aacgtgcaat
     1621 ccattgttca aaataaaacc cagcaggtcc taattccatc aatgctcgtt ttaaactata
     1681 acgtgctgca aacgtaccat ttaccttttt caattgattg aatgccagtt gataaatagc
     1741 tttggttgac attccatcat gaatgcgggg caacatattt tctaaaactt cggccacttc
     1801 aatatcggat gcacccgatt ttgtgagtga atatttgagt ttttctacgt caaattcaga
     1861 tacttcgcca gtttgtcgtt ttattttaaa attcatagtc aattagagtg aacgtgccaa
     1921 tttaaaatag tgcatcaatt gtgtggtttg aataattttt tgccacgaat cggctttcca
     1981 ttcggcaacg gattgaaaat aattttgcat taggtcggca gttcgttgtt gctcattcat
     2041 tgcctccaat ggcacaatta gttcttgcga tggtggaaaa cgatacaaca atcgattggc
     2101 ataactccta acatcaatat cttttgtggt tttagcaaac aattgtaatt tttcatctcc
     2161 cacttgcgta tttgacagcc acaatatggt tattactgtt cgttgcgcct cggttgaaat
     2221 ggtatcgtat atttctgcta aaggagtcac atatttttga ggtttcaaag gcaataacaa
     2281 ataggccgca atttcacctt gattgaacgt tttattgatt aacggattca acacattgat
     2341 tttagttgtt ttaaacaatg tttttacacc attcaccaca ttaatgccac ttagcgcaaa
     2401 agttctatat ttttcaatca attcccattg gttcatgtcc gcccaattga tgtttgcaat
     2461 tgcttgttca attagtgcat tgtcagaagg tttaaacgat tgaatattaa ggtaagagca
     2521 caaatcaaaa tacaaataag aaggttgatt aggaattgtt aatacttttc taatttgagg
     2581 taaaaaatta gccgtatcct tatccatttc tttccaaaac aatcgcaatt cttcgttctt
     2641 ttttattgct tcatctggag ttaaagtagc aattttgaaa ttgtaaatag attcaacttt
     2701 ttctttaaat gattgtgaat aagtaattgt attacaaaca aataaaaaaa ctaaaaaatt
     2761 taaagcaaat ttcataatcc tactttttat aaatttaatt ctcttctttc ataccttata
     2821 ccacaattca aattcagcat gtatttcaca caaatttagt gattatttgt aacaacctat
     2881 tttttgttaa tatctcttga caacttcttg ttgttgctaa tgctataatt cttatttttg
     2941 acataaatca aaacacgatg aacaattaca aacatcaagt aagagaaaat tttattcgct
     3001 acgcgaaaat agacacaaca gcagatccta attcaacaac ttttccttct tctatgaagc
     3061 aaaaagactt agcacaattg cttgtaaaag aattgcaaga catgggggct agtgaagttg
     3121 aaatggacga atggggctat gtttttgcaa ccattccaag taacaccgac aaagtaacat
     3181 caactatctg tttttgttcg cacatggaca cagcgccaga tgtaactgga accaacgtta
     3241 aaccaattgt gcacgaaaaa tacaacgggc aacctattgt tttgccagac gatccaacgc
     3301 aaatcatcac tacggaaatg catccgtatt tgaaagaaaa aattggtgac gatatcatca
     3361 cggcaagtgg aacaacgctt ttgggtgctg acgacaaagc aggtgttgcc atcattatgg
     3421 attttgccaa ttatttatta acacatcctg aggtaaaaca cggaaaagta cgcattttat
     3481 tcacacccga cgaagaagtt ggtcgcgggg t
//
LOCUS       DAOQVQ010000222         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30518_length_3510_cov_3.272359, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000222 DAOQVQ010000000
VERSION     DAOQVQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30518_length_3510_cov_3.272359"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKN22_07240"
     CDS             <1..449
                     /locus_tag="PKN22_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNS42536.1"
                     /translation="LTMGSSVSKQMYLFRGDTDIDGMAATQKGIFKLGVKWFGKKIIG
                     EYPYKDLYMLEAARQFQPIVKNAQLILLGGINDFTHISKAFQEGFDFVAMGRALLREP
                     EIINNIIKDPYKSGLCIHCNKCMFSVYSTTNCVFTKEYATSSQQAV"
     gene            complement(528..986)
                     /locus_tag="PKN22_07245"
     CDS             complement(528..986)
                     /locus_tag="PKN22_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNS42537.1"
                     /translation="MGIVQALNLPPAPLKIEKRKGELYVWCIVRKKFLVLTPEEWVRQ
                     HFIHYLIVVVEIPIERIASEYGITVNNLTRRCDVVVFDKSGKAKMIVECKATTVEVNQ
                     SVFFQIAQYNRQLNVDFLVLTNGLTHFFARINRETNQLEMLDGIQKEWLM"
     gene            complement(986..2218)
                     /locus_tag="PKN22_07250"
     CDS             complement(986..2218)
                     /locus_tag="PKN22_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNS42538.1"
                     /translation="MKTDPELLKHLMQQGTLLPQEEMLEVANRKGSLYIGIPREISFQ
                     ENRVALVPEAVSLLVSNGHRVKIETKSGEGSKFTDRDYSEAGAEICYDREEIFKCNII
                     FKVTPPTDKEIDLMPGNQTLISALQITTQPKETLQKLMNKKITAVAWDYIRDEQGVFP
                     VVRTMGEIAGTTSVLIAGELLSSYNEGKGMMLGGVAGVQPSEVVVIGAGSVGEYATRA
                     ALGLGANVRVFDNSLSRLRRLQNEIGQRINTSVIQPKVLEKAIKRADVAIGALRASLG
                     RTPCVVTEEMVQGMKPGSVIVDVSIDQGGCFETSRITNHKKPTFVEYGVIHYCVPNIA
                     SRISRTASFALSNIFSPILLEMGNVGGCENLIANDPGFRSGVYIYKGNLTSEVLGKVF
                     DLKYKSIELLIMGLKRNQ"
     gene            complement(2369..2677)
                     /locus_tag="PKN22_07255"
     CDS             complement(2369..2677)
                     /locus_tag="PKN22_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS42539.1"
                     /translation="MKTYSLEELTDHFIGEKGTVKRDAFENELRLDLIGNAIKQARLE
                     QQLTQEELGQLVGVKKAQISKIENSATDARFTTILKVFEALGAKVSFNVELNHKKLAI
                     "
     gene            complement(2677..3024)
                     /locus_tag="PKN22_07260"
     CDS             complement(2677..3024)
                     /locus_tag="PKN22_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNS42540.1"
                     /translation="MNEYKFRVLFLEDAKEFLGNLNEKTREKVIYNIWKSRTVNDKEL
                     LKKLKGEIWEFRTKYNKMYIRLFAFWDKTDKTDTLVVSTHGIIKKTGAIPKHEIEKAE
                     SLREQYFKTKNKK"
     gene            3226..>3510
                     /locus_tag="PKN22_07265"
     CDS             3226..>3510
                     /locus_tag="PKN22_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNS42541.1"
                     /translation="MSIVGREFPSIEVKAIDKSGSTFTLDVFEKAVSEKKKIVLFWYP
                     KDFTFVCPTELHAFQAAVAEFEKRNTLVIGASCDSAEVHFAWLTTEKNKGG"
BASE COUNT         1079 a          700 c          564 g         1167 t
ORIGIN      
        1 aattgacaat gggttcttct gtatctaaac aaatgtatct tttccgtggt gatacagata
       61 ttgatggaat ggcagctact caaaagggaa tttttaaatt aggtgttaaa tggtttggta
      121 agaaaataat tggagaatat ccttataaag acctatacat gttggaggct gctagacaat
      181 ttcaacctat tgtaaaaaat gctcaactta ttttgttagg cggaatcaat gattttaccc
      241 atatttcaaa agcgtttcaa gaaggatttg attttgttgc gatgggtaga gctttgttga
      301 gagaaccaga aatcatcaac aacattatca aagatcctta taaaagtgga ttgtgcattc
      361 actgcaataa atgtatgttt tcggtgtatt caactaccaa ttgtgtattc acaaaagaat
      421 atgcaacttc ttctcagcaa gcagtttaaa ttagcagtta aacaaattta aaaatagtaa
      481 ccatcattaa cttttaggag ttggtgatgg tttttttatt cttcaactta catcaaccac
      541 tctttttgaa tcccatccaa catctccaac tgattggttt cacgattaat gcgtgcaaaa
      601 aaatgcgtca acccattggt gagcaccaaa aaatcaacgt tcaattgacg attgtattga
      661 gcaatttgaa aaaaaacgga ctgatttacc tccaccgtgg ttgctttaca ttcaacaatc
      721 attttggctt tgcccgattt atcaaacacc accacatcgc accgtcgggt tagattattg
      781 accgtaatgc catattccga agctatgcgt tcaatcggaa tttcaaccac cacaattaaa
      841 taatgaataa aatgttgacg tacccattct tcgggagtaa gtaccaaaaa tttcttgcga
      901 acgatgcacc aaacatacaa ttcgcccttg cgtttttcga tttttaaagg agctggagga
      961 agattcagcg cttgaacaat ccccattatt gattgcgttt taatcccata atcaacaact
     1021 cgatgctttt gtattttaag tcaaagactt ttccaagcac ctcactggtt aaatttcctt
     1081 tgtagatata aacgccactt ctaaaacctg ggtcgttggc aattaagttt tcgcaaccac
     1141 ccacatttcc catttccaat aaaataggtg aaaaaatatt ggacaaagca aacgatgctg
     1201 ttctggaaat gcgcgatgca atgttaggca cacaatagtg aattactccg tattcaacaa
     1261 aagttggttt tttatgattg gtaatgcgtg aggtttcaaa acaccctcct tggtcgatgc
     1321 taacatccac aattaccgaa cctggcttca ttccctgcac catttcttcg gttaccacac
     1381 aaggtgttct tcccaacgat gcacgcaagg caccaatggc aacatcggca cgttttattg
     1441 ctttttccaa tacttttggt tgaatcaccg aggtattgat gcgctgacct atttcatttt
     1501 gtaaacgtcg caagcgcgat agagaattgt caaacacacg cacattggca cccaagccca
     1561 aggcagcacg tgtggcatat tcaccaaccg aaccagcacc aataaccacc acttctgagg
     1621 gttgcacacc tgccacacct cccaacatca ttcctttacc ttcgttgtaa gaagacaaca
     1681 attcaccagc aattaatacg gatgttgtac cagcaatttc ccccatggtg cgcaccaccg
     1741 gaaaaactcc ttgctcatcg cgaatataat cccaagctac tgctgtaatc tttttattca
     1801 tcaatttttg caaggtttct ttgggctgcg ttgtaatttg caaagcagaa atcagcgttt
     1861 gattgcccgg cattaaatca atttctttat cggttggtgg tgttacctta aaaatgatgt
     1921 tgcatttaaa aatttcttca cggtcatagc aaatttcggc acctgcctcg ctgtaatcac
     1981 gatcagtaaa cttggagcct tcaccacttt ttgtttcaat ttttacacgg tgaccgttgg
     2041 aaactaacag agaaaccgct tcgggtacca acgccacacg attttcctga aatgaaattt
     2101 cgcgcggaat tccaatgtat aaacttcctt tgcgattggc aacttccaac atttcttctt
     2161 gtggcaacaa agttccttgc tgcatcaagt gtttcaataa ttcaggatcg gttttcatat
     2221 ctctctttgg ggttaattcc acttacgaat atacgaccaa aaagcgtgaa aatcgaatat
     2281 tttctgttat tttttaacaa atcgcctttt gaaaacttat ttttaacgtg ggaagtggaa
     2341 gaattacttt ctaaaagcaa ttcctaactt aaattgctaa ttttttatga ttcaattcta
     2401 cattaaagct cacttttgcg cctaaggctt caaaaacttt caaaatcgtt gtgaaacgag
     2461 catcagttgc actgttttca atcttggaaa tttgtgcctt cttcacgcca acaagttgac
     2521 ccaactcttc ttgagttaac tgctgttcta gtcgagcttg tttaatggca tttccaatta
     2581 aatcaagtct taattcattt tcaaacgcat cgcgcttaac agttcctttt tctccaataa
     2641 aatggtctgt caactcttct aaactatacg ttttcattat tttttgtttt ttgttttaaa
     2701 atattgttct cttaaacttt ctgctttttc aatttcatgt tttggaatag ctccagtttt
     2761 ctttattatt ccatgtgttg atacaaccaa agtatctgtt ttatctgttt tatcccaaaa
     2821 agcaaaaagt cgtatataca ttttattata cttagttcta aactcccata tttcaccctt
     2881 taatttcttt aaaagttctt tatcattaac agtccgtgac ttccaaatgt tgtaaataac
     2941 tttttcccta gtcttttcgt ttagattgcc taaaaactct ttagcatctt ctaaaaatag
     3001 tacacgaaat ttatattcat tcatttggca gtagctttac acaaaaataa atgtttctta
     3061 tttaggaaac aagttttttg atttttttct ctatttttat aaagttcaca caaattccct
     3121 cccataaatt ttatttatac gattataaac atttattatg aaaactttga gaaaagtaac
     3181 gtatctttgg aatgaaattt ttgtaaaaca ttaaaataaa aagatatgag cattgtagga
     3241 agagaattcc catctatcga agtaaaagca atcgataaat ctggaagcac atttacactt
     3301 gacgtgtttg aaaaagcagt aagcgaaaaa aagaaaatcg ttttgttttg gtatccaaaa
     3361 gacttcacgt ttgtttgtcc aactgaatta catgctttcc aagctgcagt tgcagaattt
     3421 gaaaaaagaa atacattggt aattggtgct tcttgcgata gcgctgaagt acactttgct
     3481 tggttgacaa ctgaaaaaaa taaaggtgga
//
LOCUS       DAOQVQ010000223         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30544_length_3507_cov_3.757532, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000223 DAOQVQ010000000
VERSION     DAOQVQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30544_length_3507_cov_3.757532"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKN22_07270"
     CDS             <1..549
                     /locus_tag="PKN22_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42542.1"
                     /translation="FKVGKDEKPSYLQLSFQNLGIGFINKNIRNYSMDTVLHHDGYTL
                     SDIINGTTIFGKDKNIGDELGLKRDTVSKVVALPFTFQIGKIIDEHNTKMFQFYYGAK
                     VYVQNGSLPLIYAGAHYRSKKWFRLGLGASYGGFTGFRANLYVQGVWKQFNVGLATSD
                     LIGMIGLGRGYAASLNLSYRFN"
     gene            556..2019
                     /locus_tag="PKN22_07275"
     CDS             556..2019
                     /locus_tag="PKN22_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42543.1"
                     /translation="MKALIILFFLVVAFSINAQTPFYKQFSGSGYDYGQGIDELPDSS
                     YIITGSSSSFTDGPSQMFLLKIDSLGNYLWSSSYGGTETDWGRRVKHVANEGYFVGGF
                     TNSSGNGAYDFALWKIDETGNELWFKTFGTDGWERMHDMAITSDSGFVLVGETNKTTD
                     GLTDVYVVRTDASGNELWTKQFANDGEDNAFSIKMLDDSTFIVAGTRFIGTKNEAWFM
                     RLQDNGDTLWTNHHSINSFDFKVNDIEIVSGVIYGIGAIKDNVNNTYNHHIILKVNAT
                     NGSLIGSDISGSNYMQGQAITTFAQNGLFCVATSMFEEGVSFGQDDVIFYGYNNIPVY
                     YGNIGAVEYATQQVLGDMVTTYSFGAIAVGYNEEIGPGGSSVYVIRLKPWSSFYTSND
                     DFTTNPLVNIQSINTDNSAVVVYPNPASSVINVQTKKSNDYQIQLIDVLGNLVFNQTY
                     MSTKQLSIDATHLENGIYLLQLISNEGKSTLKIEIAK"
     gene            complement(2121..2447)
                     /gene="ssb"
                     /locus_tag="PKN22_07280"
     CDS             complement(2121..2447)
                     /gene="ssb"
                     /locus_tag="PKN22_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNS42544.1"
                     /translation="MTTIRNHVTLIGNIASERRTTQFENGNKIVRFDVATDKEYRANN
                     GKMKSTQEWHKVFAWGNIANFIERFGEKGKKVAIHGRLITRSYFSPEGVKRTITEVEV
                     KHILGL"
     gene            complement(2460..2771)
                     /gene="ssb"
                     /locus_tag="PKN22_07285"
     CDS             complement(2460..2771)
                     /gene="ssb"
                     /locus_tag="PKN22_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNS42545.1"
                     /translation="MNHVNLIGKMCSAPKIVELTNGRKLAKFSMSTNEMVLTADGETK
                     KRTQWHRMSAWGKWVKILEEMGEAGMELAIEGKLITHFYNSNGKRKCVSEVEVNDLVI
                     L"
     gene            complement(2843..3187)
                     /locus_tag="PKN22_07290"
     CDS             complement(2843..3187)
                     /locus_tag="PKN22_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNS42546.1"
                     /translation="MNNLRNKVSLIGRLGMQPEVTTTSNGKTLARFSLAINSSYKDKD
                     GNWKEETQWMNINAWGNVAELVKKLLSKGQEIVVEGRLVNNSYESKTGEKRFSTNIEM
                     NEFLLLTPKADK"
BASE COUNT         1073 a          592 c          636 g         1206 t
ORIGIN      
        1 tttaaagtag gtaaagatga aaaaccatct tacttgcaat taagctttca aaacttaggc
       61 ataggtttta tcaataaaaa cattagaaat tattcgatgg atactgtttt gcaccacgat
      121 ggttacacct tgtcggatat tattaatgga acaaccattt ttggaaaaga taaaaacatt
      181 ggtgatgaat tgggcttgaa aagagatacc gtttctaaag ttgtcgcttt gccatttacg
      241 tttcaaattg gtaaaatcat agatgaacac aataccaaaa tgtttcagtt ttactatggt
      301 gctaaggtgt atgtgcaaaa cggatcgttg ccgttgattt acgctggagc ccactaccga
      361 agtaaaaaat ggtttcgttt aggattgggc gcttcttatg gaggctttac aggttttaga
      421 gcaaatttgt atgtgcaagg tgtgtggaaa caattcaatg ttggattggc aaccagtgat
      481 ttaattggga tgattggact tggaagagga tacgctgcat cattgaattt aagttatcga
      541 tttaattaat aaattatgaa agcattgatt attttattct ttctggttgt agccttttca
      601 ataaatgcac aaacaccgtt ctacaaacaa ttctcgggta gtggctacga ttacggtcaa
      661 ggaattgatg aattacccga ttcgagttac atcattacgg gttcatcctc tagttttaca
      721 gatggccctt ctcaaatgtt tttgcttaaa attgatagcc taggcaatta tttatggtca
      781 agtagttatg gaggcactga aaccgattgg gggagaaggg taaaacatgt tgccaatgaa
      841 ggttattttg ttggaggttt cactaattca tcaggcaatg gtgcgtatga ttttgcattg
      901 tggaaaattg atgaaacagg caatgaattg tggttcaaaa catttggaac agatggttgg
      961 gagcgcatgc acgatatggc tatcacctct gatagcggct ttgtattggt tggtgaaacc
     1021 aataaaacaa ctgatgggtt aactgatgtt tatgttgtta gaactgatgc cagtggaaat
     1081 gagctttgga ccaagcaatt tgccaacgat ggcgaagaca atgctttttc cattaaaatg
     1141 ttggacgatt ctactttcat agttgctgga acacgtttta ttggcaccaa aaatgaagct
     1201 tggttcatgc gtttgcaaga taacggtgat accttgtgga ccaatcacca ttccatcaat
     1261 tcatttgatt ttaaagtaaa tgatatcgaa attgttagtg gtgtcattta tggaattggt
     1321 gcaattaaag acaatgttaa caacacctac aatcaccata ttattttaaa agtaaacgct
     1381 accaatggtt cattaatcgg ttcggatatt tcaggaagta attacatgca aggacaagca
     1441 atcaccacat ttgctcaaaa cggtttgttt tgtgttgcca catcaatgtt tgaagaaggt
     1501 gtttctttcg gtcaagacga tgtcattttt tatggttaca ataatatacc agtatattat
     1561 ggcaacattg gcgcggttga atatgctact caacaagtgt tgggagatat ggtaacaacc
     1621 tatagttttg gtgcaatagc tgtaggatat aacgaagaaa ttggtccagg cggttcaagt
     1681 gtgtatgtta ttcgcctaaa accttggtcg tctttttaca cttccaatga tgatttcaca
     1741 accaatcctt tggttaatat tcagtctatt aataccgata attctgccgt tgttgtttat
     1801 cctaatccag caagtagtgt aatcaatgtt cagacaaaga aatcaaatga ttatcaaatt
     1861 caattaattg atgtattagg aaatttagtt ttcaatcaaa cttatatgtc aacaaagcaa
     1921 ttgtctattg acgcaactca tttggaaaat ggaatttatt tacttcaatt aataagtaat
     1981 gaaggcaaat caaccttaaa aattgaaatc gctaaataac agtatttata atttataaat
     2041 tgaaaaaacc aatggaacgg tcaatgtcca ttggtttttt tagttgtaag ataggaggtt
     2101 ctatttgaaa ggagtgaaaa ttacaagccg agtatgtgtt ttacttctac ttcggttatt
     2161 gttcttttca ccccctctgg actgaaatac gagcgggtta tcaaacgtcc atgtatcgcc
     2221 actttctttc ccttctctcc aaaacgctcg ataaaatttg caatgttgcc ccaagcaaac
     2281 accttgtgcc attcttgagt gcttttcatt ttaccattat ttgctctgta ttctttgtcc
     2341 gttgcaacgt caaaacgcac aattttgttt ccgttttcaa attgggtagt tcttctttca
     2401 gaagcaatgt tgccaatcaa tgttacgtga tttctaattg ttgtcatatc gctctgtttt
     2461 taaagaatta ccaaatcatt tacttccact tcagaaacac acttgcgttt tccgttgcta
     2521 ttgtagaaat gggtaatcaa tttaccttct atagccaatt ccattcctgc ttcgcccatt
     2581 tcttccaata ttttcaccca tttcccccaa gcactcattc ggtgccattg tgttcttttt
     2641 tttgtttctc catctgccgt taataccatt tcattggttg acattgaaaa ctttgctaat
     2701 tttcgtccgt tggttaactc aacaattttt ggtgccgagc acatttttcc gattaaattt
     2761 acgtgattca tacttgtttg tttttagtgt ggggagtaat acgttgatcg gtcaaactcc
     2821 ccacgtcatt gttactattt aattatttgt ctgcttttgg cgttaagagt aaaaattcat
     2881 tcatctcaat gttggttgaa aatctttttt cacctgtttt gctttcatag ctgttgttca
     2941 ctaagcgccc ttcaaccaca atttcttgtc cttttgataa aagttttttt accaattctg
     3001 ccacattacc ccatgcatta atattcatcc attgggtttc ttctttccaa ttaccatctt
     3061 tgtctttgta agaagaatta atcgcaagtg aaaaacgcgc taacgttttt ccattacttg
     3121 ttgttgtaac ttctggttgc atacctaagc gaccaatcaa actaactttg tttcttaaat
     3181 tgttcataat gtaaaatttt aaaagtttga aaaatttgtt tattgctgcc gattaccatc
     3241 aatcgacgat gcaaagttgg aggagcaaca aaaaaacatt cggttaataa ctaattgtag
     3301 tcgaaaaaca ttcgtttgta aacgataaaa accgaataaa acacagaaaa acaagttgat
     3361 aaagaaaaat tctaaatatt taaaaaccat aaaataatga ttgtaaattt gaaataataa
     3421 aaaaatgggc ataaaaaaag gaatccgaag actcacgaat ggtcggaaga aattccggcc
     3481 attttttatg ttttaaaaaa tagcttt
//
LOCUS       DAOQVQ010000224         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30584_length_3504_cov_3.537257, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000224 DAOQVQ010000000
VERSION     DAOQVQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30584_length_3504_cov_3.537257"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..478)
                     /locus_tag="PKN22_07295"
     CDS             complement(17..478)
                     /locus_tag="PKN22_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS42547.1"
                     /translation="MVIIDETSVESIYYNATNRPTELDKHGIIKFVSSQFSSDTNSKE
                     NIRKALDYATKERMSFGGFVIVQKWFGEIIGVSIVNHTGMEGYMPENILVYIAVAEQH
                     QNKGLAKEMIQQVLNYAKGEVAVYLRDRNDLIPAFEHLGFKKTIEGSVIVR"
     gene            complement(640..1230)
                     /gene="nadD"
                     /locus_tag="PKN22_07300"
     CDS             complement(640..1230)
                     /gene="nadD"
                     /locus_tag="PKN22_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNS42548.1"
                     /translation="MKIGLYFGSFNPIHVGHLIIANHMVENTDLDQVWFVVSPHNPFK
                     EKKTLLADHHRLALVKEAIDDNPKLRASDVEFHLEQPNYTVKTLMVLKENYPSHTFVL
                     LMGEDNLASFHKWKNYEYILEHYQLFVYPRVHSEELTVAENNIATHPSVTLLKDVPIM
                     NISSTFIRNGIKNGKDVRYLLTPPVAQYVDEMGFYK"
     gene            complement(1227..>1577)
                     /locus_tag="PKN22_07305"
     CDS             complement(1227..>1577)
                     /locus_tag="PKN22_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNS42549.1"
                     /translation="EVYTDNFYGTLKSELERIWAKGHCVIFDVDVIGGLNLKKILGDS
                     ALAVFVQPPSVEVLKERLKSRSTETEEKINMRISKAEYELSFSNQFDQIILNEHLETA
                     CNEALKIVTDFIKQ"
     assembly_gap    1578..1587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1588..1816)
                     /locus_tag="PKN22_07310"
     CDS             complement(<1588..1816)
                     /locus_tag="PKN22_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNS42550.1"
                     /translation="MSTLNKLGKSIIFSAPSGAGKTTLVHYLLEQLPQLEFSISACSR
                     APRTNEVDGKDYYFIGVEGFKKKVEEDAFLVW"
     gene            complement(1816..2682)
                     /locus_tag="PKN22_07315"
     CDS             complement(1816..2682)
                     /locus_tag="PKN22_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNS42551.1"
                     /translation="MLMSMTGFGRSTNTFESKKISVEVRSLNSKGLDLNVRIHNYYKE
                     LEPEVRKKVTELLERGKIDLYINLDSTGDSKNFSINTELAAAYFKDLQAINAAIGQKT
                     DDYLSVLMRMPDIYINEQKEMTDDEKQFIIKLVEDACQKLNEFRRQEGIALENEFTQR
                     IENIRRLLNEVPKFEKERIETVKERIRKGLEEISGANYDQNRLEQELIFYIEKLDVSE
                     EKMRLANHLDYFLETMKVVNAGKKLGFIAQEIGREINTLGSKSNHAEMQKLVVDMKDD
                     LEKIKEQVLNTL"
     gene            complement(2782..2979)
                     /locus_tag="PKN22_07320"
     CDS             complement(2782..2979)
                     /locus_tag="PKN22_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42552.1"
                     /translation="MNATQMHMIYYNRKRAEEALNQHKKLGAASKLSIIEVAQLMDIL
                     QINTNKPNQHSVQKLAPIHWN"
     gene            complement(3019..>3504)
                     /locus_tag="PKN22_07325"
     CDS             complement(3019..>3504)
                     /locus_tag="PKN22_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNS42553.1"
                     /translation="VSTAGVSKMIKKLGDDGVKFNFALSLHAANDEKRSKIMEINDTN
                     DLESLKEALAYFYEKTGTRVTFEYIIFKDFNDSIEDARELVVFSRCVPCKINIIEYNP
                     IDDAGFQRADPKKTDEFAAYLESKGLVVNIRRSRGKDIDAACGQLANKNKAVAERTFK
                     N"
BASE COUNT         1043 a          700 c          523 g         1228 t
ORIGIN      
        1 cttttcttga aatttattac cgcacaataa cacttccctc tattgttttc ttaaagccta
       61 agtgttcaaa tgctggaatt aaatcgtttc tatctcttaa ataaactgca acctcgcctt
      121 ttgcataatt caaaacttgt tgaatcattt cttttgccaa acctttattt tgatgttgct
      181 ctgcaactgc aatgtacact aaaatatttt cgggcatata gccttccata cctgtatgat
      241 taacaattga aacaccgatt atttcaccaa accatttctg aacaattaca aaaccaccaa
      301 aagacatgcg ttctttggtt gcataatcca acgcttttcg tatgttttct ttactattgg
      361 tgtctgaaga aaattggcta ctaacaaact taatgatgcc atgtttatcc aactctgttg
      421 gcctgttcgt tgcattgtaa taaatgcttt ctacagaagt ttcatcaata attaccataa
      481 ccaagtagtt taaaagttac ttaagtcttc ttcaataatt aatgattatt taggatattg
      541 caatttaagc attttatatt gatttttgaa taaaaaggct cgttatatgt gcaaattaca
      601 cgtgaaaatg aatatttatt gctatttaac gaaagtttgc tatttgtaaa atcccatttc
      661 gtccacatat tgagcaacag gtggcgttaa aagataacgc acatcttttc cattttttat
      721 cccattgcgg ataaacgtag atgaaatatt cattattgga acatctttta ataaggtaac
      781 agatggatgt gtcgcaatgt tgttttcagc gactgttaac tcttctgaat gaacgcgagg
      841 ataaacaaaa agttggtaat gctctaaaat gtattcgtag tttttccact tatgaaacga
      901 tgccaaattg tcttcaccca tcaaaagcac aaaagtatgc gaaggataat tttctttaag
      961 caccatcaat gttttaaccg tataattggg ttgctctaag tgaaattcaa cgtcggatgc
     1021 ccttaatttt ggattatcgt caattgcttc tttcaccaaa gccaaacgat ggtgatcggc
     1081 caaaagtgtt tttttttcct taaacggatt gtgtggcgaa accacaaacc atacttggtc
     1141 caaatcggta ttctcaacca tgtgattggc aataatcaaa tgaccaacat ggatgggatt
     1201 aaacgaacca aagtacaatc ctattttcat tgctttataa aatcggttac aatttttaat
     1261 gcttcgttgc aagccgtttc taaatgttcg ttgagaataa tttgatcaaa ttgattgcta
     1321 aaactcaact catattcagc cttgcttatg cgcatattaa ttttttcttc ggtttctgtg
     1381 gaacgcgatt tcaaacgttc tttcaacacc tcaacagatg gcggttgaac aaaaacagcc
     1441 aatgcactat cgcccaaaat cttttttaag tttaaacctc caattacgtc cacatcaaaa
     1501 ataacacaat gtccttttgc ccaaatgcgt tccaattcgg atttcaaagt gccataaaaa
     1561 ttatcggtat acacttcnnn nnnnnnnccc ataccaaaaa agcatcttcc tctacttttt
     1621 tcttaaaccc ttctactcca atgaaatagt aatctttgcc atctacttca ttggtgcgtg
     1681 gcgctctgct acatgccgaa atggaaaact ctaattgtgg aagttgctcc aataaataat
     1741 gcaccaacgt ggttttaccc gctccggatg gtgctgaaaa aataatactt ttacctaatt
     1801 tatttaaagt tgacattaca atgtgttcaa tacttgttct ttaatttttt ctaaatcgtc
     1861 tttcatatca acaaccaatt tttgcatttc ggcatggttg cttttacttc ctaaagtgtt
     1921 gatttcgcga ccaatttctt gggcaataaa ccctaatttc ttacctgcat tcactacttt
     1981 cattgtttcc aagaaataat ccaaatggtt agctaaacgc attttttctt cagaaacatc
     2041 caatttttca atgtaaaaaa tcaattcttg ctccaaacga ttttggtcgt agttggcacc
     2101 agaaatttct tctaatcctt tgcgaatacg ctcttttact gtttcaatac gttctttttc
     2161 aaactttgga acttcattca ataaacgacg aatgttttca atgcgttgag taaattcgtt
     2221 ttccaatgct attccttctt gacggcgaaa ttcgttcaac ttttggcaag catcctccac
     2281 caattttatg ataaactgtt tttcatcatc cgtcatttct ttttgttcgt tgatataaat
     2341 atcgggcatg cgcattaaaa ccgataaata atcatctgtt ttttgtccaa ttgcagcatt
     2401 aattgcttgc aaatctttaa aataagctgc tgctaattca gtattgatgg aaaagttttt
     2461 ggaatctcct gtgctgtcca aattaatgta taaatcaatc tttcctcgct ccaataactc
     2521 ggtaactttt tttctaactt ctggctctaa ctctttgtaa tagttatgaa tacgcacgtt
     2581 taagtccaac cctttgctgt ttaacgaacg cacttcaact gatatttttt tagattcaaa
     2641 cgtgtttgtg gaacgtccaa atcccgtcat tgacattaac atagacctaa tttttgacaa
     2701 agctactcaa aaaaaatgtg tttgacgaat ttcaactcca aacgaccggc ttaaaattag
     2761 agaatgtgct atacaagtcg attaattcca atgaattgga gccaattttt gcacagaatg
     2821 ttgattgggt ttgttggtat tgatttgaag aatgtccatc aattgggcca cttcaataat
     2881 agaaagttta cttgcagcac ctaacttttt gtgctggttc aacgcttcct ctgccctttt
     2941 tcggttgtaa taaatcatgt gcatttgtgt cgcattcaat tgcaacagtt tacgaaatga
     3001 tgctcaaata gttacaactt aattcttaaa ggttctttca gctaccgctt tgtttttatt
     3061 ggccaattgt ccgcatgccg catcaatgtc ttttcctcga ctacgtcgaa tattcaccac
     3121 caagccttta ctttctaaat aggcagcaaa ttcatcggtt ttctttggat cggcacgttg
     3181 aaaacctgcg tcatcaattg gattgtattc aataatgttg attttgcacg gaacacaacg
     3241 agaaaaaaca accaattcac gtgcatcctc aatggaatca ttgaaatctt tgaaaataat
     3301 gtattcaaat gttacgcgtg tccccgtttt ttcataaaaa taagccaatg cctctttgag
     3361 cgactctaaa tcattggtat cattgatttc catgatttta ctgcgtttct catcgttggc
     3421 agcatgcaat gaaagtgcaa aattaaattt cacgccatca tcgcccaatt ttttaatcat
     3481 tttcgaaact cctgctgttg aaac
//
LOCUS       DAOQVQ010000225         3502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30609_length_3502_cov_3.289527, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000225 DAOQVQ010000000
VERSION     DAOQVQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30609_length_3502_cov_3.289527"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PKN22_07330"
     CDS             complement(<1..1635)
                     /locus_tag="PKN22_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNS42554.1"
                     /translation="MKVQVLVLTALLSLSNGVFAQSNIIPTPERFLSTKAAAYRFHTI
                     AFEQENLNQEELAVMQSWAAKFTPPNSGKQATVLNISFDPFKTPQSNADFYEASFVNN
                     KVIVRYTSNASKLYAVSSILQLIDQKNDELFITPFKIQDKAKFSWRGLHLDVARHFFN
                     VDELKRFIDIMAFYKYNKFHWHLTDDQGWRIEIKKYPKLTEIGAFRDSTVNGHYSDVP
                     RTYKKERYGGFYTQEQIKEVIQYAAARSVTVVPEIEMPGHARAAIAAYPELSCTKQQM
                     PVEGLWGVFDDIFCSDEKTVNFLKDVLTEVAALFPSEYIHIGGDEAPKTRWKACEVCQ
                     NNIKKNNLKDEHELQSWFIREIDAFMTKKGKKIIGWDEILQGGLSPNATVMSWQGNEG
                     GILAAAQGHDVIMTPVNFCYFDYYQAESRYEPLAIGGLVNLEKVYSFGVIPSEISPEN
                     RKHILGAQANLWTEYIPTYDKVEYMEYPRALAMSQNLWSLDKGSYEDFVKAIVEKQSS
                     VLKAFKINYSQSFARPTLQPSLIENGVRLYGTSPNKK"
     gene            complement(1689..>1868)
                     /locus_tag="PKN22_07335"
     CDS             complement(1689..>1868)
                     /locus_tag="PKN22_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNS42555.1"
                     /translation="AKVKSKMLLQVHDELIFDVHKDEIEKMSDLIHNAMEKAVNLVVP
                     MKAEVGVGQNWLEAH"
     assembly_gap    1869..1878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1892..>3502)
                     /locus_tag="PKN22_07340"
     CDS             complement(1892..>3502)
                     /locus_tag="PKN22_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNS42556.1"
                     /translation="FKTIETEKPNYQLIQTANDRKELLDLLLQQTEVCFDTETTGLDA
                     LHADIVGMSFSFKAKEAFYVALPLHFEEAKSIVEEFAPFFTSSSIVKIAHNAKYDIKV
                     LNRYGIKVAYPMFDTMLAHYLISPESKQNMDFLAEFYLGYKPISIEELIGKKGKNQGN
                     MGDLSPEEVYNYACEDADITFQLKQIFAPEIEKEHLKSLFYDMEMPLVEILAAMEIEG
                     INIDQSFLNDYSKTLGGLLDNLEKEIKTLAGMDFNVDSPKQLGDVLFEHLKITSKAKK
                     TKTGQYATSEDVLDGLKDAHPIVPKILEYREYRKLKSTYVDPLPTLADEVDGRVHTQF
                     MQTVTATGRLSSNNPNLQNIPVRTTAGREIRKSFIPKNEDYVIISADYSQIELRIIAA
                     LSEDENMINAFKAGQDIHAATAAKVYGIPLEEVTKEQRSSAKAVNFGIIYGQSAFGLS
                     QNLGISRTEAKQIIDSYFEQYPTIRAYMDKQIAQAREKGFVETIFKRRRYLPDINSAN
                     AVVRGFAERNAINAPIQGSAADIIKLEH"
BASE COUNT          986 a          667 c          639 g         1200 t
ORIGIN      
        1 ttttttgttg ggagaagttc catataaacg caccccgttt tcaataagtg atggttgcaa
       61 agttggacgt gcaaacgatt gcgaataatt gatcttaaac gcttttaaaa cagaactttg
      121 tttttcaact attgctttaa caaaatcttc ataactacct ttgtctaagc tccataggtt
      181 ttgactcatt gccaaagcac gagggtattc catgtattca actttgtcat aagttggaat
      241 gtattctgtc cacaaattgg cttgtgcacc aagaatatgt tttctgtttt cggggctaat
      301 ttcacttggg ataacgccaa aactatacac cttttctaaa tttactaaac caccaatagc
      361 tagtggttcg tatctgcttt cggcttggta ataatcaaaa tagcaaaaat taacaggtgt
      421 cataataaca tcgtgacctt gagcagcagc taaaattcca ccttcgtttc cttgccagct
      481 cattacagtg gcgtttggac ttaaaccacc ttgtaaaatt tcatcccaac caatgatttt
      541 tttccctttt ttggtcataa aagcatcaat ttcacggata aaccagcttt gcaattcgtg
      601 ttcgtctttc aaattgtttt ttttgatgtt attttggcaa acttcacatg ctttccaacg
      661 tgttttaggt gcttcatcgc caccaatgtg aatgtattct gatggaaaca aggctgctac
      721 ttctgtcaaa acatctttta aaaagttgac cgttttttca tccgaacaaa aaatatcgtc
      781 aaaaacaccc cacaaacctt caacgggcat ttgttgtttg gtgcaagaaa gctcaggata
      841 ggctgcaatt gccgcacgtg catgtccagg catttcaatt tctggaacaa cagtaacact
      901 tcgtgctgct gcatattgaa ttacttcttt tatttgttct tgggtgtaaa aacctccgta
      961 acgttctttt ttataagtgc gtggcacatc gctgtaatgg ccgtttactg ttgaatctct
     1021 aaatgctcca atttctgtaa gttttggata ttttttgatt tcaattctcc aaccttgatc
     1081 atcggttaag tgccaatgaa atttgttgta tttgtaaaac gccatgatgt caataaaacg
     1141 ctttagttca tcaacattaa aaaaatgacg agcaacatct aaatgcaacc ctctccaact
     1201 aaattttgct ttgtcttgaa ttttaaatgg agtgatgaat agttcgtcat ttttttggtc
     1261 aattaactgc aaaatggagc tcactgcata caatttacta gcattggatg tataacgaac
     1321 aatcactttg ttgttgacaa aagacgcttc gtagaaatca gcatttgatt gaggtgtttt
     1381 gaagggatca aaactgatgt ttaaaacagt tgcttgcttg cccgagtttg ggggagtaaa
     1441 tttagctgcc cacgattgca ttacagcaag ttcttcttgg tttaaatttt cttgttcaaa
     1501 tgcaatggta tggaaacgat atgcagctgc ttttgttgat agaaagcgtt ctggcgttgg
     1561 gataatgttg ctttgtgcaa aaactccatt gctcaatgat aacaatgcag ttaaaactaa
     1621 aacttgtact ttcatgtgtt ttgttttaca attaaaattt gtttcttttt aagtgaaagt
     1681 gattggtttt aatgggcttc taaccaattt tgtccgacac caacttcggc tttcattggt
     1741 accactaagt taaccgcttt ttccattgcg ttgtgaatta aatccgacat tttttcaatt
     1801 tcatctttgt gaacatcaaa aattaattcg tcgtgcactt gcaacagcat tttggatttt
     1861 actttggcnn nnnnnnnnga acgaaatgct atcaatgctc caatttgatg atatcggcag
     1921 ccgaaccttg aattggagca ttgatagcat ttcgttcggc aaaaccacgc acaacggcat
     1981 tggcagaatt gatatcgggc aaataacggc ggcgtttaaa aatagtttct acaaatcctt
     2041 tttcacgtgc ttgtgcaatt tgtttgtcca tgtaggcacg aatggttgga tattgctcaa
     2101 aatagctgtc gattatttgt tttgcttcgg tgcgcgaaat gcctaaattt tgtgacaaac
     2161 caaaggcaga ttgaccgtaa ataattccaa aattgaccgc ttttgcagag ctacgttgtt
     2221 cttttgtaac ttcttccaac ggaattccat acacttttgc agcggttgca gcgtgaatat
     2281 cttgtccagc tttaaaagca ttaatcatat tttcatcctc acttaaagcg gcaataatgc
     2341 gcaattcaat ttgcgaataa tcggcagaaa tgatcacata atcttcattt ttagggatga
     2401 acgacttacg aatttcacga cctgctgttg tgcgtacagg aatgttttgc aagttgggat
     2461 tgttagaact caaacgccct gtagcggtta ctgtttgcat aaattgtgtg tgtacgcgac
     2521 catctacttc gtctgccaac gttggaagcg gatctacata agtgcttttc aacttgcggt
     2581 attcgcggta ttctaatatt tttggaacaa tagggtgggc atctttcaat ccatccaaca
     2641 catcttcgga agtggcatat tgtcctgttt ttgttttctt ggctttggag gtaattttta
     2701 aatgttcaaa caacacatcg cccaattgtt taggcgaatc tacattgaaa tccattccag
     2761 ccaaggtttt gatttctttt tccaaattat ccaacaatcc gcccaatgtt ttggagtaat
     2821 cgttcaaaaa agattgatca atattgattc cttcgatttc catggcagcc aaaatttcaa
     2881 caagtggcat ttccatgtcg taaaacaacg atttgaggtg ttctttttca atttctggtg
     2941 caaaaatttg ctttaattga aaggtgatat cggcatcctc gcaagcgtaa ttgtacactt
     3001 cttctggcga taaatcgccc atatttcctt ggtttttgcc ttttttgcct attaattctt
     3061 caatagaaat gggtttgtat cctaagtaaa attctgctaa aaagtccatg ttttgtttgg
     3121 attctggact aattaaataa tgcgccaaca tggtatcaaa catcggatag gcaaccttaa
     3181 ttccgtagcg atttaaaacc ttaatgtcgt atttggcatt gtgggcaatt ttcacaattg
     3241 aagaggaagt gaaaaaaggt gcaaattctt caacaatcga tttagcttcc tcaaaatgga
     3301 gtgggagcgc cacataaaac gcttctttcg ccttaaaact aaacgacatt ccaacaatat
     3361 cagcatgcaa tgcatccaaa cctgttgttt cggtgtcaaa acatacttcg gtttgttgaa
     3421 gcaataaatc caacaattct ttacggtcgt ttgccgtttg aataagttgg tagttaggct
     3481 tttctgtttc tattgtttta aa
//
LOCUS       DAOQVQ010000226         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30662_length_3497_cov_3.427368, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000226 DAOQVQ010000000
VERSION     DAOQVQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30662_length_3497_cov_3.427368"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKN22_07345"
     CDS             <1..938
                     /locus_tag="PKN22_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNS42557.1"
                     /translation="LLPMSNKPAYSIAEQIALLEQRGMLFKDENKAHHFLENISYYRL
                     KGYWWDVQSDVKFHTFHSNTYFEDIIDRYNFDRRLRLLLFDAIERIEIALRTKMIYYL
                     SIRYGSLWYLDASLFNSATVTIKGVSKTIHQNTIDELHKEFNRSQEIFIKDHRKRFPN
                     QEADAWKILEVASMGTLSKLYKNINHQLPEKALIAKAMGLNLHSELSSWLEAITYVRN
                     IIAHHSRLWNRNMVKRPIVNINNPIGSWFEHPLTEVQQKKPFLIISCMIYLCNEVTPG
                     HQIKSKILELFENNPTIPIYKMGFLNNWSKEQLWK"
     gene            1249..1968
                     /locus_tag="PKN22_07350"
     CDS             1249..1968
                     /locus_tag="PKN22_07350"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNS42558.1"
                     /translation="MNSVVVDAGNTAIKIGFFIEGELQQFSRISWDDFMAENPLTEKI
                     KKYKGIFSSVLTPEKNQQIREHYPDLIQFSSQHKLPIELDYKTPASLGVDRLCNAIAA
                     WRLNPNKNSLIVDVGTCLKFDFVSQNGIYQGGSISPGIRLRYQSLNDYTANLPLLSAV
                     DKTNLIGKSTTESIHSGVINGINAEIERLIQQYEENFDSLTIFVTGGDAKYFDFDLKN
                     NIFVTENLTLYGLYETYLFNA"
     gene            1961..3304
                     /locus_tag="PKN22_07355"
     CDS             1961..3304
                     /locus_tag="PKN22_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42559.1"
                     /translation="MHKNIVALFILCCAFVANAQINNPYTSFGVGERTNSLHPVFSGM
                     GNQSVTYVHPSVLNVSNPASYSFLRYQYPIFSLGVSSRLSFNNDNGVKEFNNATNISE
                     IAFGMSFAKRFGIAFGFKPMYKKNYSFVETKKLIDDSVRYEYEGKGALNNAFVGFSIN
                     LLNYDSLKWSIGGNVGALFGAMTDERRSSIVSTLTNAGGNETRRQQVKSFYYEVGTLL
                     EYRFKKENKLTVGASVEPMQNIKSVYDRQLSYSATDVTNPNTFQLLSESGEIKGKIVF
                     ATSYSVGASYARYITTNKKDGNKGKAHLTFSLGYNATDFSKYKEVYDTVTYSYGFNNT
                     ASYQFGMQFIPETQIVGSAIPKLFERSSYRLGFHYSTLPYMHLGKNVNEWAITCGLGL
                     PVLVDKRLDSSIQLGIGVGQRGTANALKENFVTINLGLLIAPSINDRWFIKRKVD"
     gene            3313..>3497
                     /locus_tag="PKN22_07360"
     CDS             3313..>3497
                     /locus_tag="PKN22_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42560.1"
                     /translation="MKFKSFGIVSVLFVLLFSCTNDLNSIKKITYDPKSPNEVSVNLN
                     IYYADSGYAKLNIFAAHA"
BASE COUNT         1169 a          569 c          606 g         1153 t
ORIGIN      
        1 ttttactacc tatgtcaaat aaacctgcat acagcatagc tgaacaaata gctttattag
       61 aacaacgtgg aatgttgttt aaagatgaaa ataaggcaca tcatttttta gaaaacataa
      121 gttattaccg tctaaaaggt tattggtggg atgttcaaag tgatgttaaa ttccatacat
      181 ttcactcaaa tacatatttt gaagatatta ttgatagata caattttgat cgtcgtttaa
      241 gattactatt atttgacgcc attgaacgga ttgaaattgc tttgcgaaca aaaatgattt
      301 attacttatc gataaggtat ggaagtttgt ggtatttgga tgcaagttta tttaattcag
      361 caacagttac aattaaagga gtaagcaaaa caattcatca aaatacaatt gacgagttgc
      421 acaaagaatt taatagaagt caagagattt ttataaaaga tcatcgaaaa cgttttccca
      481 atcaagaggc agacgcttgg aagattttgg aagtagcatc gatgggaact ctttcaaaat
      541 tatataaaaa catcaaccat caactcccag aaaaagcatt gattgccaaa gctatggggt
      601 tgaatttaca tagtgaatta tcaagttggt tagaggcgat tacctatgtt cggaatataa
      661 ttgctcacca ttcacgtcta tggaatagaa acatggtgaa acgaccaatt gtaaacatca
      721 ataatcctat aggtagttgg tttgaacacc cattgacaga agtacagcaa aaaaaacctt
      781 ttttaataat ttcttgtatg atttatttgt gtaatgaagt aactcctggt caccaaataa
      841 aatcaaaaat tttagaatta tttgagaata atccaactat tccaatatat aaaatggggt
      901 ttttaaataa ttggagcaag gaacagttgt ggaaataatg gaaagttagt atccactttt
      961 gtcttgattc ctcattgcgc tcattgcggt ttaaaaaaag cttaaccgcg gagttacgcc
     1021 aagaacacgc agagttcgca gagataaaca ttgcgtccat tgcggttatt atttaattcc
     1081 tctttgcgtt cattgcgcct tctttgcgct cgttgcggtt atacttcaat tcccctttgc
     1141 gttcattgcg ccttctttgc gctcgttgcg gttaaaaaca tttatcgaaa ctacttgctt
     1201 cttgcttttc aataatatta atttactatt tttgttacta tgaataaaat gaatagtgta
     1261 gttgttgatg ctggaaatac ggctattaaa attggctttt ttatagaagg agaattgcaa
     1321 caattttcac gaatttcatg ggatgatttt atggcagaaa accctttaac agaaaaaatt
     1381 aaaaaatata aaggaatttt ctcatctgta ttaactcccg aaaaaaatca acaaattaga
     1441 gaacactatc ctgatttaat acaattcagc tcccaacata aactaccaat tgagttggat
     1501 tataaaactc cagcatcttt aggagttgat cgactctgta acgcaattgc agcttggcga
     1561 ttaaatccca ataaaaattc attaattgtt gatgttggta cgtgtttaaa atttgatttt
     1621 gtatcccaaa atggcattta tcaaggggga tcaattagcc ctggcatacg cttgcgctac
     1681 caatcgctaa atgattatac cgccaatttg cccttgcttt ctgctgtaga taaaacaaat
     1741 ttaatcggaa aatcaaccac agaatcaatc cattctggtg taatcaatgg aataaatgcc
     1801 gaaatcgaac gtttaattca acaatatgaa gaaaatttcg attcattaac tatttttgtg
     1861 actggtggag atgcaaaata ctttgatttc gatttaaaaa ataacatctt tgtaaccgaa
     1921 aatttaacct tatatggttt atacgaaact tatttattca atgcataaaa acatagttgc
     1981 cttattcatc ctttgttgcg cttttgtagc aaatgctcaa attaataatc cttatacatc
     2041 ttttggtgtt ggtgaacgca caaattcttt acatcccgta ttttctggaa tgggaaatca
     2101 aagtgtaacc tatgttcatc cttctgtttt gaatgtatcc aaccctgcat cttattcttt
     2161 tttaagatac caatatccaa tcttttcttt aggtgtttct agtcgtttgt cgttcaataa
     2221 cgataacgga gttaaagaat ttaacaatgc aaccaatata tctgaaattg cttttgggat
     2281 gtcgtttgcc aaacgtttcg ggattgcctt tgggtttaaa ccaatgtata agaaaaacta
     2341 ttcgtttgta gaaactaaaa aattaattga tgatagcgtt cgttatgaat acgaaggaaa
     2401 aggagcgctt aacaatgctt ttgttggatt ttcaatcaat ttattgaact acgattcttt
     2461 aaaatggtca attggtggaa atgttggtgc tctttttgga gctatgaccg atgagcgtag
     2521 aagctctatt gttagtacac ttaccaatgc tggaggaaac gaaacaagac gtcaacaagt
     2581 caaatcgttt tattacgaag ttggaacctt gctagaatac cgctttaaaa aggaaaataa
     2641 attaaccgtt ggagcaagtg ttgaaccaat gcaaaacatc aaatcggttt acgatagaca
     2701 attgtcttat tctgcaaccg atgtaaccaa tccaaatacc tttcaattgt tatcagaatc
     2761 gggtgaaata aaaggaaaaa ttgtttttgc aacttcgtat agcgtaggag cttcttatgc
     2821 gcgttacatt acaactaata aaaaagatgg aaacaaagga aaagcacatt taactttttc
     2881 tcttggttac aacgcaactg acttctcaaa atacaaagaa gtgtacgata cggtgactta
     2941 ttcgtatggg tttaacaaca ccgctagcta ccaatttgga atgcaattca ttccagaaac
     3001 acaaattgtt ggtagtgcta tccctaaatt atttgagcgt tctagttacc gtttaggttt
     3061 ccattattct acattgccct acatgcactt aggcaaaaat gtgaacgaat gggctataac
     3121 ttgtggatta ggcttgcctg ttttggttga taaacgcttg gattcttcta ttcaattagg
     3181 aattggtgtt ggtcaaagag gtactgctaa tgcgttaaaa gaaaattttg ttaccatcaa
     3241 tttaggattg ttaattgctc caagcatcaa tgatagatgg ttcattaagc gtaaagtgga
     3301 ttaatggttg acatgaagtt taaatctttt ggaatagtat ccgtattatt tgtgctatta
     3361 ttttcatgca ccaatgattt aaacagcatt aaaaaaatta catacgaccc caaatcgccc
     3421 aacgaagttt ctgtgaattt aaacatctat tatgccgatt cgggatatgc caaattaaac
     3481 atttttgctg ctcatgc
//
LOCUS       DAOQVQ010000227         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30833_length_3482_cov_4.456959, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000227 DAOQVQ010000000
VERSION     DAOQVQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30833_length_3482_cov_4.456959"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..923
                     /locus_tag="PKN22_07365"
     CDS             144..923
                     /locus_tag="PKN22_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNS42561.1"
                     /translation="MSTAQAVDQGHVEFSIAASGIATIEFGHPLSNSLPGKILQKLAD
                     TITELGNNDTVKVIVIRSSGEKAFCAGASFDELISIKDLETGKQFFSGFAKVINACRK
                     APKLIIGRVHGKAVGGGVGVASAVDYCFATSNADVKLSELAVGIGPFVVGPAVERKIG
                     VSAMSELAINATEWRSAEWAYRKGLYAQLFETAAEMDAEIDVLATKLANSNPEAMAML
                     KQIFWQGTENWDELLAERAGMSGKLVLSDFTVNAINQFKKK"
     gene            990..1925
                     /locus_tag="PKN22_07370"
     CDS             990..1925
                     /locus_tag="PKN22_07370"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNS42562.1"
                     /translation="MLQKTIILTAFLSSLICFGQKDSVPTVHTINSSPQTFTFNKGRT
                     IGVSVGVGAAWAGSMIGLHQVWYKGMEKAPFHTFDDSREWMQMDKMGHFYTANKISNL
                     VGDMYTWAGWQNKHAALLGFGVGFGYQFTLECLDAMGQRWGFSWSDMGANTLGAGMYL
                     AQQLAWKEQRFILKFSYHNSPYAQYRPNTLGGTFAERLLKDYNGQTYWMSISPGAFLK
                     NSKFPKWLCFSVGYGVEAKLHGIDDVYTVSTGNSALTFKAQRQVLFSLDIDFSKIPIK
                     NKWLKAIVKQFNYLKLPFPTLIVTGNKWSGKWLYF"
     gene            complement(1922..2404)
                     /locus_tag="PKN22_07375"
     CDS             complement(1922..2404)
                     /locus_tag="PKN22_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42563.1"
                     /translation="MFGIFKNKKKQPEAEVKQEIVALKPIDYEAKIILAWAKAVEGDA
                     KFTLWLKDNYFELFMATQAIYLKEEARDWLMKNGYAHLMAFINAAEGNEVAQKWLKAH
                     QFDLLYHMALAIESEQESWTWLLKNATQDLFILTKAIKEVKDKIEENHNDIHSFRKDL
                     "
     gene            complement(2411..>3482)
                     /locus_tag="PKN22_07380"
     CDS             complement(2411..>3482)
                     /locus_tag="PKN22_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNS42564.1"
                     /translation="LCLWMGIMKVGEEGGAVKLLSKAVSPLFSKLFPEVPKDHPAVGS
                     IMMNFSANMLGLDNAATPIGLKAMKELQEINPDKERASNAQIMFLVINTAGLTIIPVS
                     ILAFRSAAGSSSPSEIFIPILFTTFFSLLIGISVVAIKQKINLFNKTVIAYLGGMSAL
                     LVGLLLLIVYKPQYTEYVSKVGGNIFILAIIVLFIVLAMRKKINVYETFIDGAKGGFQ
                     VAIDIIPYLVAILAAIALLRSSGSLDAFQDGIKWCLYNIGVHADAVVDALPVAYMRPF
                     SGSGARGLMLEIFDTYGVDSFTAKLAATFQGSTETTFYVLAVYFGSVKIKNTRYAAGA
                     GLIADIAAAIIAIIMALLFYPH"
BASE COUNT         1102 a          621 c          717 g         1042 t
ORIGIN      
        1 aagtgaaaac actggttctt cttctgaaag agttttgcgt ccacgtagaa gaacaacaga
       61 aggtggggaa gaaagaactt acgaaaaagt ggagcgtttt actaaaagaa tagacgataa
      121 caaagaataa taaattaaaa cttatgtcaa cagcacaagc agtagatcaa ggacacgtag
      181 aattttcaat tgcagcatct ggtattgcaa ctattgagtt tgggcatcca ttgagcaact
      241 cgttacctgg taaaatctta caaaaattag cagataccat tacagaatta ggaaataatg
      301 ataccgtaaa agtgattgtt attcgttctt cgggtgaaaa agcattttgt gcaggagcta
      361 gctttgatga attgatttcc atcaaagatt tggaaactgg aaaacaattt ttctctggat
      421 ttgctaaggt gattaatgct tgtagaaaag cacccaaatt aattattggt cgcgtgcacg
      481 gaaaagctgt tggtggtggc gtaggtgtag cttcggctgt agattattgt tttgcaacta
      541 gcaatgcaga tgtaaaactt tctgagttgg cagtaggaat cggaccattt gtggttggac
      601 ctgcggttga acgtaagatt ggtgtttcag caatgagcga gttggctatc aatgcaactg
      661 aatggcgctc tgctgaatgg gcttatcgta aagggttgta tgctcaattg tttgaaacag
      721 ctgctgaaat ggatgctgaa attgatgtgt tggcaaccaa attggcaaat tctaacccag
      781 aagcaatggc tatgttgaaa caaatatttt ggcaaggaac agaaaattgg gacgaattgt
      841 tggctgaaag agctggtatg agcggaaaat tggtgttgag cgattttacg gtgaatgcca
      901 tcaatcagtt taaaaagaaa taaacgtttt cttaaaaata taaatccctc ggtattttta
      961 tcgggggatt ttttttgtac tttcgttttg tgttacagaa aactatcata ttaaccgcat
     1021 ttttatcttc actgatttgt tttggacaaa aggatagtgt tccaactgtt cacacaataa
     1081 attcatcacc ccaaacattt acatttaata aaggcagaac cattggagta tctgttggtg
     1141 ttggtgcagc atgggcagga agcatgatcg gcttgcacca agtgtggtat aaaggaatgg
     1201 aaaaagcacc ttttcacact tttgatgatt cacgcgagtg gatgcaaatg gataagatgg
     1261 gacattttta tacggccaat aaaatttcaa atttggttgg tgatatgtac acctgggcag
     1321 gttggcaaaa caaacatgct gcattgctag gttttggagt tggttttggc tatcaattta
     1381 ccttagaatg tttggatgct atgggacagc gttgggggtt ttcatggagc gatatgggag
     1441 caaatacttt gggagctggg atgtatttag cgcaacaatt ggcgtggaag gagcaacggt
     1501 ttatattgaa attttcttat cacaattccc cctatgcgca ataccgtcca aacactttgg
     1561 gaggcacttt tgcagaacgt ttattgaaag attacaacgg tcaaacctat tggatgagca
     1621 ttagcccggg agcttttttg aagaattcaa aatttccaaa atggttgtgt ttttctgttg
     1681 gttatggagt tgaagccaaa ttgcacggta tagacgatgt ttatacggtc agtactggaa
     1741 attctgctct aacatttaaa gcacaacgcc aagttttgtt ttcgttggac attgatttta
     1801 gtaaaatacc aattaaaaac aagtggttaa aagcaattgt aaaacagttt aattacctta
     1861 aattaccttt cccaacgctt atcgttacgg gcaacaaatg gagtggtaaa tggctgtatt
     1921 tctaaagatc ttttctaaac gagtgaatat cgttgtggtt ttcttcaatt ttatctttca
     1981 cttccttgat agcttttgtg agaatgaata aatcttgagt agcatttttg agcaaccaag
     2041 tccaactttc ttgttcactt tcaatggcca atgccatgtg gtacaataaa tcaaattgat
     2101 gggcttttaa ccatttttgc gccacttcat tgccttctgc tgcattgata aacgccatga
     2161 ggtgtgcata tccgtttttc atcaaccaat cgcgggcttc ttcctttaga taaatggctt
     2221 gtgtagccat aaaaagctca aaataattgt cttttaacca caaagtaaac tttgcatcgc
     2281 cctcaactgc ttttgcccat gctaatataa ttttcgcttc ataatcaatg ggttttaaag
     2341 caacaatttc ttgttttact tctgcttcgg gttgcttttt tttattttta aaaatgccaa
     2401 acatagttca tcaatgagga taaaataata acgccataat gatggcaatt attgcagcag
     2461 ctatatcggc aattaatcct gcgccggctg cataacgggt atttttaatt tttacagagc
     2521 caaaataaac cgccaacaca taaaacgttg tttcagtgct tccttggaaa gtggccgcca
     2581 attttgcggt gaatgaatct acgccatagg tgtcaaaaat ttccaacatc aaaccacgtg
     2641 cgcccgaacc gctaaatgga cgcatgtagg caactggaag agcatcaaca acagcatcgg
     2701 catgcacgcc aatgttatac aaacaccatt ttattccatc ttgaaaggca tccaacgaac
     2761 ccgaagaacg caacaaagca atggccgcca aaatagccac taagtagggg ataatgtcaa
     2821 tagctacttg aaatccaccc tttgcgccat caataaaagt ttcataaaca ttgatttttt
     2881 tacgcatggc aagtacaata aacaaaacaa tgatggccaa aataaaaatg ttgcctccta
     2941 cttttgaaac gtattcggta tattgtggtt tgtaaacaat taagagcaat aagccaacca
     3001 ataaagccga cattccaccc aaataggcga taacagtttt attgaaaagg ttaatttttt
     3061 gtttgattgc taccaccgaa ataccaatta aaagcgagaa aaaggtggta aacaaaatag
     3121 gaataaaaat ctctgaaggg gaggaggagc ctgctgccga acgaaacgcc aaaatagaaa
     3181 ccggaataat ggttaaacca gcagtgttta tcaccaaaaa catgatttgt gcattgcttg
     3241 ctcgttcttt atcaggatta atttcttgca attctttcat ggcctttaaa ccaatgggag
     3301 tagcagcatt gtctagtccc aacatattgg cgctaaaatt catcataata gaacctacag
     3361 ctggatgatc tttgggtact tctggaaaca atttggagaa caacggagaa acagcttttg
     3421 ataacagttt aacagcgcca ccttcttcgc ccactttcat aattcccatc cacaaacaca
     3481 ag
//
LOCUS       DAOQVQ010000228         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30841_length_3482_cov_2.734753, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000228 DAOQVQ010000000
VERSION     DAOQVQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30841_length_3482_cov_2.734753"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..870
                     /gene="aqpZ"
                     /locus_tag="PKN22_07385"
     CDS             181..870
                     /gene="aqpZ"
                     /locus_tag="PKN22_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNS42565.1"
                     /translation="MRKLVAEFIGTLWLVLGGCGSALISAAYPELGIGFLGVALAFGL
                     TVVTMAYAIGHISGCHLNPAVSVGAWIAGRLDKKDLIPYIAAQVLGAIAGAGILYLLA
                     SGKHGFEIGGFAANGYGEHSPGGYNMTAAFLSELVMTFIFLIVILSSTHSKAPSGFAG
                     LAIGLTLTIIHLVSIPITNTSVNPARSTSQAIFVGDWAINQLWLFWLAPIVGAILAGL
                     VYKFFLDEKVD"
     gene            983..1804
                     /gene="pyrF"
                     /locus_tag="PKN22_07390"
     CDS             983..1804
                     /gene="pyrF"
                     /locus_tag="PKN22_07390"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNS42566.1"
                     /translation="MNKASIISQIKKKGSFLCVGLDPDLSKIPAHLLNTEDPIFEFNK
                     AIIDATKDFCIAYKPNTAFYECYGVKGWQSLQKTIDYIPNDCLIIADAKRGDIGNTSD
                     YYAKTFFQELGADAVTVAPYMGEDSVKPFLQYENKWVIVLALTSNAGALDFQFTTDAS
                     GEELYKKVLKKVAAWGSPENTMFVVGATRAEGIGEVRKIVPNNFFLVPGVGAQGGSLE
                     DVANYGWNNDCGIIVNSSRGIIYASNGLDFAQKAAESAQQMQAEMRQILLQKGFI"
     gene            complement(1786..2619)
                     /locus_tag="PKN22_07395"
     CDS             complement(1786..2619)
                     /locus_tag="PKN22_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS42567.1"
                     /translation="MKKYTNLIYQGSDNRSSLYDLFLVDDSKDQALVVFIHGYTGYKD
                     WGCWNLMGDAIANNGYSFAKFTITHSATTVEHSTEFVDLEAFGNGGYLKELRDLQYFL
                     DHLEKTHGFKKFILVGHSRGGGAVILGGNDERVKQIHCLAPISDIAARFPKGEKMEEW
                     KKTGVYFHKNSRTGTELPHYFCQYEDFLAHQEELTIEIAAKKLQKPVFVYHGSDDTSV
                     EPWEGKLVAEWTNGTYYNIEGTAHTFDTKEPWTENKMPETFAKVVQMIVTTISNQINP
                     F"
     gene            complement(2652..>3482)
                     /locus_tag="PKN22_07400"
     CDS             complement(2652..>3482)
                     /locus_tag="PKN22_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS42568.1"
                     /translation="PLLTEEDAIISDALNHASIIDGVRLCKAQRFRYANADMAELEEQ
                     LKAAQSCRFRLIVTDGVFSMDGNVCPLDKIYELANRYNAMIMVDESHSAGVVGKTGRG
                     VTELYQLRGKVEILTGTLGKAFGGAVGGFTTGKKEIIDLLRQRSRPYLFSNSLAPSIV
                     GAANKVFDLLSSTTELRDRLEENTNYFKKHIIEAGFDIKPGDSPIVPIMLYDAALSQQ
                     FADELLKEGVYAIGFFYPVVAKGQARIRTQISAAHTKEDLDKAINAFIKVGKNLGVIK
                     "
BASE COUNT         1009 a          662 c          660 g         1151 t
ORIGIN      
        1 ttaaaggtta ataaatacaa aaaagtcgga agaaatttct tccgaccatt cgtgccaatt
       61 tggtgacctt tttgtttttt tattatattt ataacctcaa ctcatctgat acttagtgtt
      121 tttttaaatt tcctattgtt taaaatggga ctaatgttta attttttaaa atataaaagt
      181 atgagaaaat tagtagctga atttattgga accctatggt tggttttagg tggatgtggg
      241 agtgccctta tatctgctgc ctatcctgaa ttaggaatag gttttttagg tgtggcgtta
      301 gcctttggtt taacagttgt aacaatggcc tatgcgattg gacacatttc aggatgtcat
      361 ctaaatcctg cggtatcagt tggggcttgg attgcgggtc gtttggataa aaaagattta
      421 attccctaca tcgctgcgca agttttgggc gctattgctg gggcaggaat cttgtattta
      481 ttggcaagtg gaaaacatgg ttttgaaatt ggtggttttg ctgctaacgg ttacggtgaa
      541 cattctccag gaggttacaa tatgactgca gcttttcttt ctgaattagt aatgaccttt
      601 atcttcttaa ttgtcatctt aagttctaca cattcaaaag caccaagtgg ttttgcaggc
      661 ttagcaattg gtttaacgtt aactatcatc catttagtga gtattccaat taccaatacg
      721 tctgtcaacc ctgcaagaag tactagccaa gcaatttttg ttggcgattg ggctataaat
      781 caactttggt tgttttggct ggctcctatt gttggtgcaa tattagctgg tttggtttat
      841 aagtttttcc ttgacgaaaa ggtagattaa taagtaaaat attcaataat gaagaggact
      901 tttagttctc ttttttgttt tattccaatc ttgttctaga aggatgtaat tttctgtcta
      961 tatttgcacc ataattagca atatgaacaa agcatcaatt atttcgcaga tcaaaaagaa
     1021 aggttctttc ttgtgtgtgg gcttagaccc tgatttatca aaaattccag ctcatttgtt
     1081 aaacacagaa gacccaattt ttgagttcaa caaagccatt attgatgcaa caaaagattt
     1141 ttgtattgcc tacaaaccca acacagcatt ttatgaatgt tacggtgtca aaggatggca
     1201 atctctgcaa aaaaccattg attacattcc caatgattgt ttgattattg ccgatgcaaa
     1261 acgtggcgat attggcaata cttcggatta ttatgccaaa acattttttc aagaattagg
     1321 tgccgatgcg gttacagttg caccatacat gggcgaagac agtgtaaaac cttttttaca
     1381 atacgaaaac aaatgggtta ttgtgttggc tttaacgtct aatgctggtg ctttggattt
     1441 tcaattcaca acagatgctt cgggcgaaga attgtataaa aaagtcctta aaaaagttgc
     1501 tgcttggggt tctcccgaaa atacaatgtt tgtagtagga gcaacccgtg ccgaaggaat
     1561 tggtgaagtg cgcaaaattg ttcccaataa tttcttttta gttccgggtg ttggtgcaca
     1621 aggtggatcg ttggaagatg ttgccaatta tggatggaac aacgattgtg gtatcattgt
     1681 caactcttcg cgcggtatta tttatgcgtc aaatggactt gattttgccc aaaaagcagc
     1741 tgaaagtgca caacaaatgc aagcagaaat gcgtcaaatc ttacttcaaa aaggatttat
     1801 ttgattagaa atagtggtga ctatcatttg aacaacttta gcaaacgttt cgggcatttt
     1861 attttctgtc caaggttctt tggtgtcaaa tgtatgtgcc gttccttcta tgttgtaata
     1921 agtgccgttg gtccattcag ccactaattt gccttcccat ggttcaaccg atgtgtcgtc
     1981 gcttccatgg taaacaaata cgggtttctg caactttttt gcggctattt caatggttaa
     2041 ttcttcttga tgtgccaaaa aatcttcgta ttgacaaaaa tagtgcggta attcagtacc
     2101 cgttctactg tttttatgaa agtagacacc cgtttttttc cattcctcca ttttttcacc
     2161 ttttggaaaa cgcgctgcaa tgtcagatat aggtgctaaa caatgaattt gctttactcg
     2221 ctcatcattt ccacccaaaa taaccgctcc accaccacgc gaatgtccaa ctaaaatgaa
     2281 ttttttgaac ccatgtgttt tttctaggtg atctaaaaaa tactgcaaat cgcgcaattc
     2341 ttttaaatat ccgccatttc caaaagcttc taaatccaca aattcagttg agtgttcaac
     2401 agttgtggca ctgtgcgtaa tggtaaactt ggcaaacgaa taaccattat ttgcaattgc
     2461 atcgcccatt aaattccaac aaccccaatc tttgtagccc gtgtagccgt gaataaacac
     2521 aacaagagct tggtcttttg agtcatcaac taaaaacaag tcatacaacg atgaacggtt
     2581 gtcagaacct tggtaaatta aattcgtata ttttttcata acaaaaaagg ctcctcgatg
     2641 gagcctttgt cttatttgat aacacctaga ttttttccta ctttgataaa cgcgttgatt
     2701 gctttgtcca aatcttcttt ggtgtgagca gccgatattt gcgttctgat gcgtgcttgc
     2761 cccttagcaa caacagggta gaagaagcca attgcataca ccccttcttt taacaattca
     2821 tctgcaaatt gttgtgataa tgctgcatca tacaacataa ttggcacaat tggagaatcg
     2881 cctggtttga tgtcaaaacc tgcttcaata atgtgttttt tgaaatagtt ggtattttct
     2941 tccaatcggt ctctcaattc tgttgttgag ctcaacaaat caaatacttt attggcagca
     3001 ccaacaattg atggagcaag tgaattggaa aacaaataag gacgagaacg ttgacgcaac
     3061 aaatcgataa tttctttttt gcccgttgta aatccaccaa ctgcgccacc aaaggctttg
     3121 cccaaagttc ctgttaaaat ttccacttta ccacgtaatt ggtacagttc agtaactcca
     3181 cgtcctgttt taccaaccac accagccgaa tggctttcat caaccataat catggcatta
     3241 tagcgattgg ctaattcgta tattttgtct aacggacata cattgccatc cattgaaaac
     3301 acaccatcag tcacaatcaa tcgaaaacga caggattgtg ccgctttgag ttgctcttct
     3361 agctccgcca tatctgcatt tgcgtaacga aaacgctgtg ccttacataa acggacacca
     3421 tcgataatgg aagcgtgatt caaagcatcg gaaataatag catcttcttc tgtcaacaag
     3481 gg
//
LOCUS       DAOQVQ010000229         3478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_30887_length_3478_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000229 DAOQVQ010000000
VERSION     DAOQVQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30887_length_3478_cov_3.666667"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKN22_07405"
     CDS             complement(<1..333)
                     /locus_tag="PKN22_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNS42569.1"
                     /translation="MGAIGVFDSGYGGLTILQSFLQKLPEHDFIYYGDNQRAPYGTRS
                     FEEVYDFTLESVVKLFEKGCPLVILACNTASAKALRTIQQKYLPLHYPDRRVLGVIRP
                     SAEVVGQLS"
     gene            445..1731
                     /locus_tag="PKN22_07410"
     CDS             445..1731
                     /locus_tag="PKN22_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS42570.1"
                     /translation="MKLIVTLFMAFTVGWSWSQETYNNCNNALELCPNNPVTVNNYSA
                     NKTFCPNCEDDFTFCMTPNNSIWLKFTTNELGGDIQVNFNNPLFQSQVGQDNRYNAAL
                     IQANIPCNAASYTQVGNCVTTATGNQVINALGLPANTAYYIVLSGEKSGAGITLAAEF
                     SMTVAVSGTAIDRPVPTLYLGIEDTICANTLAFVSGSRTNCTDAGEFRWYLNNQLIAT
                     TTDSIIFTDKINNGDIITVETDCFTTCPLTITQTTLPLVVHSVIADAGIDRTIRPGES
                     VQIGGGITVNDTLVWLPSYALSDAQVAFPIANPQETTTYQLLVTDTTTGCRASDYITI
                     TVDKGLFFPTTFSPNGDGANDTWEILGIEAYPDCFVSIFDRWGQKVFEAVGYNKEKAW
                     NGTGKMGKLNEGVYFYKIQLHDKEKQQMTGSITLIR"
     gene            1736..2662
                     /locus_tag="PKN22_07415"
     CDS             1736..2662
                     /locus_tag="PKN22_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS42571.1"
                     /translation="MRYLFVAYLVICCSCVSLAQQIPQYSQWLNHQFAINPAHAGIKQ
                     CIDVHALFRAQWVGIKGAPMSGFATASFGLASKRKKYLSARHGMGVKFEADKIGQFNN
                     NRFNVAYAGHFNFNEYNRLSLGIYAGIVQMGYNHATSTTVSPDPVVMHETSIIRPDAH
                     FGAWWNGKNYYIGFMANQLIPTKWNLGSNSQFRFHFAFNGGYRFILSDKLGLIASSMV
                     RIPVVGPVNADFNLNFDIQNIVNLGVGFRTQDAVIFMAGFKINQQFSLNYSFDFTVSG
                     LKKVSGNTHEIGISFITCKPENRSTSRCPLFE"
     gene            complement(2665..>3478)
                     /locus_tag="PKN22_07420"
     CDS             complement(2665..>3478)
                     /locus_tag="PKN22_07420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNS42572.1"
                     /translation="FINDDAFLYLNNSLSLPQIIAVFIEVVCAFMVVFYIYHLFTSMD
                     RFSKNKLKIANELLVAQNQKIVIQNEEKSTLAKEIIHRVKNNLQIVVSLLQIQKNEIH
                     DKTSKEKINDAIQRIVAISMYHDKLFNTNKLTNKAFADLITNIFENNLKIQLKNKKFN
                     LKLDASIVEINAQTLVPIVLIINELAANSIKYESTEVEKGIIDLTISSFDENKLLIQY
                     HDNENWHFAVDGEECFGLQLINSLVEQLDGNISIDKTNGGTLFVIEVQKIND"
BASE COUNT         1081 a          635 c          637 g         1125 t
ORIGIN      
        1 ggataattgt cccaccactt cagcacttgg acgaataaca cccaacactc tcctatcggg
       61 ataatgcaag ggcaaatatt tttgttgaat ggtacgcaag gctttagcag aagctgtgtt
      121 gcaagctaga attaccaacg gacaaccttt ttcaaacaat tttacaaccg attctagtgt
      181 aaaatcatat acttcttcaa aagagcgagt tccataaggc gctctttgat tgtcaccgta
      241 gtaaataaaa tcgtgttctg gtaatttttg caagaaagac tgtagaatgg ttaaaccacc
      301 ataccctgaa tcaaaaacgc caattgctcc catgcttcaa aattacaaaa gaaacgcgaa
      361 gctcaacaat caaatactat tttttacgtt atatagtgca caaagtgcat tgaaataaca
      421 ttcaaagcat taaatttgta gagcatgaaa ttaatagtta ccctttttat ggcatttaca
      481 gtcggttgga gttggtcgca agaaacctac aacaactgca acaatgcact ggaattatgt
      541 cccaacaatc cggtaacagt caacaattac agtgctaaca aaacattttg cccgaattgc
      601 gaggacgatt ttacattttg catgactccc aacaacagca tttggttaaa atttaccacc
      661 aatgaattgg ggggcgatat tcaagttaat tttaataatc cgttgtttca atcgcaagta
      721 ggacaagaca atcgttacaa tgcagcgttg attcaagcca acattccgtg caatgctgca
      781 agttacacgc aggttggcaa ttgtgttaca acagctactg gcaatcaagt gataaatgct
      841 ctaggtttgc ctgccaatac ggcgtattac attgttttaa gtggcgaaaa atcgggagct
      901 ggcattaccc tagcagcaga attttcaatg acggttgctg tttcaggcac tgccatcgat
      961 cgtcctgttc ctacacttta tttgggtatt gaagacacca tttgtgccaa cacattggcg
     1021 tttgtttctg gaagtagaac caattgcacc gatgcgggtg aatttcgttg gtacctcaac
     1081 aaccaattaa ttgccaccac aactgattcc attattttca ccgacaaaat taacaatggc
     1141 gatataatta cagtagaaac cgattgtttt accacttgtc cactcaccat tacacaaaca
     1201 acacttccgt tggtggtgca cagtgtcatt gctgatgcag gtattgacag aaccatacgc
     1261 cctggagaaa gtgttcaaat aggtggtgga attactgtaa acgacaccct agtttggttg
     1321 ccaagctatg ctttatcgga tgctcaagtt gcttttccaa ttgccaatcc acaagaaaca
     1381 acaacgtatc aattattggt aaccgacacc acaacaggat gtagagcaag tgattacatt
     1441 actatcacag tggataaagg cttatttttt ccaaccacat tttcgcccaa tggtgatggt
     1501 gccaacgata cgtgggaaat tttaggaatt gaggcctatc ccgattgttt tgtaagcatt
     1561 tttgatcgtt gggggcaaaa agtatttgaa gctgttggct ataacaaaga aaaagcgtgg
     1621 aacggtacag gtaaaatggg caagctcaac gaaggtgttt atttttacaa aatacagttg
     1681 cacgataaag aaaaacagca aatgactgga agtattacat taattcgata actgagtgag
     1741 atatttattt gttgcatact tagttatttg ttgttcttgt gttagtttgg cacaacaaat
     1801 cccccaatat tcacaatggt taaaccatca atttgccatc aatcctgcac atgctggaat
     1861 aaagcaatgt attgatgtgc atgctttgtt tcgtgcacaa tgggttggca taaaaggagc
     1921 acccatgagt ggttttgcaa cggctagctt tggtttggca tcaaaacgca aaaaatattt
     1981 gagtgcccgt catggtatgg gggtgaaatt tgaggccgac aaaattggtc agtttaataa
     2041 caaccgtttt aatgtggcgt atgcgggtca ttttaatttt aatgaataca accgtttatc
     2101 gcttggaatt tatgctggta ttgtgcaaat gggatacaat catgctactt ccactactgt
     2161 ttcacccgac ccagtggtga tgcacgaaac cagtatcatt cgccccgatg cacattttgg
     2221 tgcttggtgg aatggaaaga attactacat aggttttatg gccaaccaat tgataccaac
     2281 taaatggaat ttaggtagca attctcaatt tcgatttcat tttgcgttca atggtggata
     2341 tcgctttatt ttgagtgata aattaggatt gatagcttcg tcgatggtgc gcattccagt
     2401 tgttggtcct gttaatgccg atttcaattt gaattttgac attcagaaca tcgttaattt
     2461 aggagttggt tttagaactc aagatgctgt aatttttatg gctggattta aaatcaacca
     2521 acaattctca ctcaactatt catttgattt caccgtttca gggttaaaga aagttagcgg
     2581 taacacgcac gaaattggaa tcagttttat tacttgcaag cctgaaaatc gttccacctc
     2641 ccgttgtccg ttgtttgaat aattttaatc gttgattttt tgcacttcga ttacaaacag
     2701 cgtacctcca tttgttttat caatacttat gttaccatct aattgttcaa ccaacgaatt
     2761 gattaattga agaccaaagc actcttcacc atcaacagca aaatgccaat tttcattatc
     2821 gtggtattga ataagtagtt tattttcatc aaaacttgaa attgttaaat caattattcc
     2881 cttttctact tcggtagatt catatttgat agagtttgca gccaattcat taataattaa
     2941 aactataggc accaatgttt gagcgttaat ttcaactata gatgcatcca gttttaaatt
     3001 gaatttttta ttttttaatt gaatttttaa attgttttca aaaatgttgg taattagatc
     3061 tgcaaatgct ttattggtta atttattggt attaaacaat ttatcatgat acattgaaat
     3121 agccacaatt cgttgtatag catcattaat tttttctttc gatgttttgt cgtgaatttc
     3181 atttttttga atttgcaaca aactcaccac aatttgcaaa ttgtttttaa cgcggtgtat
     3241 aatttctttc gctaaggttg atttttcttc gttttgaatg actatttttt ggttttgtgc
     3301 taccaataat tcgttggcaa tttttagctt gtttttagaa aatctatcca ttgaagtgaa
     3361 aagatgataa atgtaaaaaa ccaccataaa cgcacagaca acttcaataa atacggctat
     3421 tatttgaggt aatgacaaag aattattcaa atataaaaat gcatcatcat taataaaa
//
LOCUS       DAOQVQ010000230         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31016_length_3469_cov_3.073814, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000230 DAOQVQ010000000
VERSION     DAOQVQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31016_length_3469_cov_3.073814"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKN22_07425"
     CDS             <1..546
                     /locus_tag="PKN22_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42573.1"
                     /translation="FLLTLSKIIDSKGIKIQLNLYGVNKSEEIIAFIDKCNPKLKTTV
                     SIFPRIPNSDLLSKIAKENVMLLFNYYHFNGTKIYDYLGLKRQILFCFDNDKDALKLK
                     QKYFDTDECKNNNDRPQRDIIMKHNAGIIVKDQQHLYEVLENLIEEFEQNGKIGCNSD
                     NVEIYSRKIQAKHLADIIKNL"
     gene            543..1157
                     /gene="hisH"
                     /locus_tag="PKN22_07430"
     CDS             543..1157
                     /gene="hisH"
                     /locus_tag="PKN22_07430"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNS42574.1"
                     /translation="MTIAIINYGMGNLFSIEKRLKRMGVETIVASNAADLMRADKFIL
                     PGVGHFGKAMQQLQQLDLVEPLQDLVLQQKKPILGICLGMQLLANYSEEGETKGLGWI
                     NADVKRFQINDSKKYKIPHTGWNEVIPQKSSPLFENCAAPMEFYFVHSYYFNCAEEQD
                     VLAISNHEKNFVCAVQKNNIFGVQFHPEKSHEIGNTLLRNFVNY"
     gene            1158..1916
                     /locus_tag="PKN22_07435"
     CDS             1158..1916
                     /locus_tag="PKN22_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS42575.1"
                     /translation="MNRPRIIPVLLLQNGGLVKSIQFKNHQYIGDPINAVKLFNDLKA
                     DELVLLDIDATKEGRSISLDLVKQIGEEANMPFSVGGGIKDIATIQQLIQAGAERVII
                     NTAAFEQPNFIKEASSMFGTSTISVCIDYKRTLFRKEFATIKGGTTNSSYSLMEAVAF
                     AANQGAGEIIVQSIEKDGTMSGFDCTTLQKINDSVSIPVVALGGGGSKSDFESLYQAT
                     PLNGIAAGSYFVFQGKLRGVLIQYPPVSNYTFAQ"
     gene            1913..3031
                     /locus_tag="PKN22_07440"
     CDS             1913..3031
                     /locus_tag="PKN22_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNS42576.1"
                     /translation="MNTTRTYQLCNRCVMDTSDVNITFDEKGNCNHCEGYIEALQQYT
                     YRPGVSEDQWKSILATIKEKGKGKPYDCVIGISGGVDSCYTAHLCKEFGLRALLIHVD
                     NGWNTEISVKNVKTMVEKLGFDYQSCVIDWNEFKEIQLAFLKSSSVDLELPTDIAILA
                     NNYQTAVKYKIPTIISGGNFSAEGILPHQWGYHVFADFKYYNSIVKKYSKVPLKKVPT
                     VGFYGQFYYKIVKGIKTYYPLNYIQFDKDKARQFLIENYGWESYGGKHHESVITAFWQ
                     SYVMPVKFNMDYRRATLSSQICSGQVTREQAMEILKEPAYNAEKIQETKQYIAKKFGI
                     SIEELEHYLQLPPKTYVDFPNQKQFIERMFNWYKKIKG"
BASE COUNT         1235 a          550 c          641 g         1043 t
ORIGIN      
        1 tttctattga ctttatcaaa aataattgat tctaaaggaa taaaaattca attaaatcta
       61 tatggtgtca ataaaagtga agaaatcatt gcatttatag ataaatgcaa tccgaaacta
      121 aaaactactg tttccatttt tccaagaatt ccgaactcgg atttactttc aaaaattgct
      181 aaagaaaatg taatgttact tttcaattac tatcatttca acggtactaa aatttatgat
      241 tatttaggat taaaaagaca aattctattt tgctttgata atgataaaga tgcattgaaa
      301 ctaaaacaaa aatactttga tacggatgaa tgtaaaaata acaatgaccg tcctcaaaga
      361 gatattatca tgaagcacaa tgctgggata attgtaaaag accaacaaca tttatatgaa
      421 gttcttgaaa atttaattga agaatttgaa caaaatggaa aaataggatg taattctgat
      481 aatgttgaga tttattcacg taaaatacaa gcaaaacacc ttgcagatat aataaaaaac
      541 ctatgaccat cgctattatc aattatggaa tgggaaattt gttttccatt gaaaaacgcc
      601 tcaaacgcat gggagttgaa accattgttg cctccaatgc tgcagattta atgcgggctg
      661 ataagttcat ccttcctgga gttggtcatt ttggtaaagc aatgcaacag ctgcaacagt
      721 tagatttagt tgaacctctt caagatttgg tgctccaaca aaaaaagccg attttaggaa
      781 tttgcttagg gatgcaattg ctagccaatt attcagaaga aggtgaaaca aagggtctag
      841 ggtggatcaa tgctgatgtg aaacgctttc aaataaacga ttctaaaaaa tacaaaattc
      901 cacacactgg ttggaacgaa gtaattcccc aaaaatcgtc accattattt gaaaattgcg
      961 ctgctccaat ggaattttat tttgttcatt cttattactt taattgcgct gaagaacaag
     1021 atgttttggc aatttctaat catgagaaaa attttgtttg tgctgtgcaa aaaaacaata
     1081 tatttggcgt acaatttcat cctgaaaaga gccatgaaat tggaaataca ctgcttagaa
     1141 attttgtaaa ctattaaatg aaccgtccac gcattattcc ggtactcttg cttcaaaacg
     1201 gaggattggt aaagtctata caatttaaaa atcaccaata tattggcgat cccatcaacg
     1261 cggttaaact tttcaacgat ctcaaagcgg atgaattggt attgttggat attgatgcca
     1321 caaaagaagg acgaagcatt tcacttgatt tggttaaaca aataggcgaa gaagcgaata
     1381 tgcctttttc agtcggaggt ggaattaaag atattgccac cattcaacag ttgattcaag
     1441 cgggtgccga acgtgttatc atcaacacag cagcatttga acaaccaaat tttattaaag
     1501 aggcaagttc tatgtttggt acatcaacca ttagtgtttg catcgactat aaacgtacat
     1561 tatttcgaaa agaatttgca acaattaaag gtggaaccac caattcttcc tactcattaa
     1621 tggaagcagt tgcttttgct gctaatcaag gtgctggtga aattattgtt caaagcatcg
     1681 aaaaagatgg gacaatgagt ggatttgatt gcactacgct ccaaaaaatt aacgatagtg
     1741 tttccatccc tgttgtggca ttgggtggtg gtggctctaa aagtgatttt gaatccttgt
     1801 accaggcaac accgttaaat ggaattgccg caggaagtta ttttgttttt caaggaaaat
     1861 taagaggtgt tttaattcaa tatcctcccg tttcaaatta tacatttgct caatgaatac
     1921 tactagaaca tatcaactat gcaaccgttg cgtaatggac acaagcgatg tcaacattac
     1981 gtttgacgaa aaaggaaatt gcaaccattg cgaaggatat attgaagctt tgcaacaata
     2041 cacctatcgt ccaggtgttt ctgaagacca atggaagtca attctagcaa ctataaaaga
     2101 aaaagggaaa ggcaaaccct acgattgtgt aataggaatc agtggcggag tggatagctg
     2161 ctataccgct catttgtgca aagaatttgg attgcgtgcc ctccttattc atgttgataa
     2221 tggctggaat acagaaattt ctgtcaaaaa tgtcaaaact atggttgaaa aactaggttt
     2281 tgattaccaa agttgtgtga ttgattggaa cgagttcaaa gaaattcaac tggcattctt
     2341 aaaatcatct agtgtagatt tagaattacc aacagacatt gctattttgg ccaacaacta
     2401 tcaaactgct gtaaagtata aaataccaac aattatttcg ggtggtaact tctctgccga
     2461 aggaattcta ccgcatcaat ggggatacca tgtttttgct gattttaaat actacaactc
     2521 cattgtcaag aaatacagca aagttccttt gaaaaaagta cctactgttg gcttttatgg
     2581 gcagttttac tataaaatag tgaaaggcat taaaacgtat tatccgttga attacatcca
     2641 gtttgacaaa gacaaagcac gtcagttttt gatagaaaat tatggttggg aaagctatgg
     2701 aggcaagcac cacgaatcgg ttattactgc tttttggcaa tcgtatgtga tgcctgtaaa
     2761 attcaacatg gattatagac gagctacctt gtcttctcaa atttgctcgg ggcaagttac
     2821 gcgtgaacaa gctatggaaa ttttgaaaga accagcttac aatgcagaaa aaatccaaga
     2881 aacaaagcaa tacatagcta agaaatttgg aatcagcatt gaggaattgg aacattattt
     2941 acaacttcct cccaaaacgt atgtggattt tcctaatcaa aagcaattta tagaacgaat
     3001 gtttaattgg tataagaaaa ttaaagggta atttcagaaa cggttgggat ttttgaggtt
     3061 actgcgtttt ttttaaccgc aatggacgca aagaaggcgc aataaacgca aaggagagta
     3121 aaattcaaaa attggagttc caactaattt ttgagtagga atatatctcc ctccttgagg
     3181 agggattaag ggaggtgact tttgtaataa ctgaaatatt ctaacaatac actcaatatt
     3241 tatatactat tttaaattga tattcaaact aataattaaa taagagtata tctcttccga
     3301 gaagcggaaa gggattaaag gtggtgaagt gtaaagaaat aagtaagtta caacccaatt
     3361 atttacctag attccactat atatttaagg agatgcaata aaagaatgta accttcatca
     3421 agatattaaa tttaatcgtg gataatacat tgcgaaaagc agcttatgt
//
LOCUS       DAOQVQ010000231         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31020_length_3468_cov_4.509229, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000231 DAOQVQ010000000
VERSION     DAOQVQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31020_length_3468_cov_4.509229"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKN22_07445"
     CDS             <1..858
                     /locus_tag="PKN22_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS42577.1"
                     /translation="KIYPLNTGNFKLDGGAMFGVVPKSIWSKTNPADENNMCNWAMRS
                     MLIEDSGRLMLIDTGIGDKQSEKFFSHYYLHGNDSLHGNLQKLGFHADDITDVFLTHL
                     HFDHCGGAIKWNKDRTGFEPVFKNAIYWSTVNHWKWATIPNAREKASFLTENILPIQE
                     SHQLKFVQRIGDFTQNAIPNMDVLFVDGHTESMMIPHIQYKEKTVVFMADLLPSVGHI
                     PLPYVMGYDTRPLLTLSEKEKFLIEAAQKGYYLFLEHDSVNELCTVKETEKGIRLDQT
                     FSFAEIFGN"
     gene            873..2189
                     /locus_tag="PKN22_07450"
     CDS             873..2189
                     /locus_tag="PKN22_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNS42578.1"
                     /translation="MNNYLLELFKEENTVIIPGFGALTMINRATNELMLMPYLKHNDG
                     TLVKFIAAKEGIDETTAKSKIDAFVDEISSATSNGGNFELKGIGVLSKDASGDFVLSQ
                     SKEEESTNNSLEEVKPTPVETPVEVVEVAPIVEEPIIEEPIVEEVVVETPVITENSVE
                     EIQEEVQSTPVVEAVNDVANEPAIEVPSIESVVTSPIVEEPAQQAEKKETTSQMVSEE
                     EQWNDDLDVLPLNYKPERPKQPILEKAKKDGMAPHKKKTLVFAILAFLIVGGAAFLGF
                     NKDILNKIPFLASNKTEVVEQTPAVEEKVEQEEQPEEVVQETTQEAEDVPSEEPTSET
                     TPVEQTPVVEQKTPAIKQPQTPTVVTAGNLQFNESLPVQVIVGSFSVESNATRLVEKL
                     KSMGFPAGVINAGGMSMVAAASFQSMEEYQANLGQLKSLGNYWVKK"
     gene            2212..3045
                     /locus_tag="PKN22_07455"
     CDS             2212..3045
                     /locus_tag="PKN22_07455"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNS42579.1"
                     /translation="MNWTTIKEYQDITFKQCNGVARIAFNRPECRNAFRPKTVSELLE
                     ALIIAHESQEIGVVLISGEGPSPKDGGWAFCSGGDQRVRSTTGYKGEDGVNRFNILDV
                     QRQIRFMNKVVIAVVPGWAVGGGHSLHVVCDLTLASKEHAIFKQTDVDVASVDGGFGS
                     AYLARQVGQKRAREIFFLGKSYTAQEAFEMGMVNAVVPHDELEQTAYDWAQEILTKSP
                     TAVKMAKFAFNLIDDGLVGQQVYAGEATRLIYGTDEAVEGRNAFLEKRKRDFSKFPKF
                     P"
     gene            complement(3111..>3468)
                     /locus_tag="PKN22_07460"
     CDS             complement(3111..>3468)
                     /locus_tag="PKN22_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42580.1"
                     /translation="KLGIDEDKHAGVMEQFNDLMHSKVSSSPKQLSSLTSSKPNTAKD
                     NAFQSAMQNDFLKNLISKVGLSETQAKSVAGALPDLLKNMDMNTLTNLIGMFSNSKKG
                     GLTGMLGGILGGFFKK"
BASE COUNT         1149 a          581 c          733 g         1005 t
ORIGIN      
        1 aaaatatatc ctttaaatac tgggaatttt aaattggacg gaggtgccat gtttggtgtt
       61 gttcctaaat ccatttggtc aaaaacaaat cctgccgatg aaaacaatat gtgcaactgg
      121 gctatgcgca gcatgttgat tgaagatagc ggacgtttga tgttgattga cacaggtatt
      181 ggtgataaac aaagtgaaaa atttttctcg cactattatt tacatggtaa tgattctttg
      241 cacggcaact tgcaaaaatt aggttttcat gccgatgaca ttaccgatgt gtttttaacg
      301 catttacatt ttgaccattg cggaggcgcc attaagtgga acaaagatag aacggggttt
      361 gaacctgtat ttaaaaatgc aatttattgg agcaccgtca accattggaa gtgggcaacc
      421 attcccaatg cacgtgaaaa agcttctttt ttaaccgaaa atattttacc cattcaagaa
      481 agccatcagt tgaaatttgt gcaacgaatt ggcgatttta cgcaaaatgc aattcctaat
      541 atggacgtat tgtttgtgga tggacacacc gaaagcatga tgattccgca cattcaatac
      601 aaggaaaaaa cagttgtttt tatggccgat ttattgccaa gtgttggtca cattccgctg
      661 ccttatgtga tgggttatga cacacgtccg ttattgacct tgtctgaaaa agagaaattt
      721 ttaattgaag cagcacaaaa aggctattat ttgttcctag aacacgatag cgtcaatgaa
      781 ttgtgtacgg tgaaagaaac tgaaaaaggc atccgattgg atcaaacgtt ttcatttgct
      841 gaaatttttg gaaattaaaa aactatacta caatgaataa ctatttatta gaacttttta
      901 aagaagaaaa tacggttatc attcccggat ttggtgcgtt aacgatgatt aacagagcaa
      961 ccaatgaact gatgttgatg ccctatttaa aacacaacga tggcacgttg gttaaattca
     1021 ttgccgcaaa agaaggtatt gacgaaacaa cggctaaatc aaaaattgat gcttttgttg
     1081 atgaaatctc ttctgcaacc agcaatggtg gtaactttga attgaaaggg attggcgttt
     1141 tgtcaaaaga tgcttctggc gatttcgtgc tatcgcaatc aaaagaagaa gagtcaacta
     1201 ataattctct agaagaagta aaacctacac cagttgaaac tcctgttgaa gtagtagagg
     1261 tagctccaat tgttgaagaa ccaattattg aagaaccaat tgttgaagaa gttgtagttg
     1321 aaacaccagt tataactgaa aatagtgtgg aagaaattca agaagaagta caatccacgc
     1381 ctgttgttga agctgttaat gatgtggcaa atgagccagc aatagaagtg ccttcaatag
     1441 aatcagttgt tacatcacct attgttgaag aacctgcaca acaagctgag aaaaaagaaa
     1501 ctacttcaca aatggtttct gaggaagagc aatggaacga tgatttggat gtgttgccgt
     1561 tgaattacaa accagagcga ccaaaacaac ctattttaga aaaggctaaa aaagacggga
     1621 tggctccgca taaaaagaaa acgttggttt ttgcaatctt agcgttctta attgttggtg
     1681 gagcggcttt tttaggattc aataaagata ttttgaataa aataccattc ctggctagta
     1741 ataaaaccga agttgttgaa caaactccag cagtagaaga gaaagtagaa caagaagaac
     1801 aacctgaaga agttgttcaa gaaactacac aagaagctga ggatgttcct tcagaagaac
     1861 caacttctga aactactcca gttgaacaaa cacctgttgt tgaacaaaaa acaccagcaa
     1921 taaaacaacc ccaaacgcca actgttgtaa ctgcaggaaa tttacaattc aacgaatcgt
     1981 tgccagtgca agtaatcgta ggttctttta gtgtggagag caatgccaca cgtttagtcg
     2041 aaaaattaaa atcaatgggc ttccctgcgg gagttatcaa tgcgggtggc atgtctatgg
     2101 ttgcagctgc ttcttttcaa tcgatggaag aataccaagc caatttggga caactaaaat
     2161 cgttgggtaa ttattgggtt aagaaataaa ttaaagtaat aaaaataaaa aatgaattgg
     2221 acaaccatta aagaatatca agacattaca tttaaacagt gcaatggtgt ggcaagaatt
     2281 gcattcaacc gacctgaatg tagaaatgct tttagaccaa aaacagtttc agaattatta
     2341 gaagccttaa ttattgccca cgaatcgcaa gaaattggtg ttgttttgat ttctggtgaa
     2401 ggaccatcac caaaagatgg aggatgggct ttttgttctg gtggcgatca acgcgttcgt
     2461 tccacaacgg gttacaaagg cgaagatggt gtcaaccgat ttaacatttt ggatgtgcaa
     2521 cgtcaaattc gttttatgaa caaagtggtg attgctgtgg tgcctggttg ggcagttggt
     2581 ggtggtcaca gcttgcatgt ggtgtgtgat ttaacattgg cgagtaaaga acacgcaatt
     2641 tttaaacaaa cagacgtaga tgttgccagt gttgatggtg gatttggatc ggcttattta
     2701 gcacgtcaag tgggacaaaa acgagcacgt gaaattttct ttttaggaaa atcatatacc
     2761 gctcaagaag cgtttgagat gggaatggtc aatgcagttg ttccacacga tgaattggag
     2821 caaacagcct acgattgggc acaagaaata ttaaccaaaa gtccaacagc cgtaaaaatg
     2881 gcaaaatttg cattcaactt aatagatgat ggtttggtgg ggcagcaagt atatgctgga
     2941 gaagccacac gattgattta cggaacagat gaagctgttg aaggacgcaa tgccttttta
     3001 gaaaaacgaa aacgagattt ctcgaaattc ccaaaatttc cataatgaaa aaagggtgtt
     3061 tgaaaatatc agacaccctt ttttagtttt ttttcggatt tgaagtttta ttacttctta
     3121 aaaaatcctc cgagtattcc acctaacata cctgtcaacc ctcctttttt agagttggag
     3181 aacatgccaa ttaagttggt gagagtattc atatccatat ttttcaataa gtcgggtaat
     3241 gcacctgcaa cacttttagc ttgcgtttcc gataatccta cttttgaaat taaatttttc
     3301 aaaaaatcgt tttgcattgc cgactgaaaa gcgttgtctt ttgcagtgtt gggctttgat
     3361 gatgttagac tggaaagctg ttttggtgaa ctgcttactt ttgaatgcat taagtcattg
     3421 aattgttcca tcacaccagc gtgcttgtct tcatcaattc ccagtttt
//
LOCUS       DAOQVQ010000232         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31141_length_3459_cov_2.821680, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000232 DAOQVQ010000000
VERSION     DAOQVQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31141_length_3459_cov_2.821680"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKN22_07465"
     CDS             complement(<1..228)
                     /locus_tag="PKN22_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNS42581.1"
                     /translation="MKKYIVFLLALVFFVSCKEQPKVVEELPSDSIFNLTSNWQDQNG
                     KDLKLADLRGKTLVVVMIYTTCKSACPILVSD"
     gene            complement(237..1088)
                     /locus_tag="PKN22_07470"
     CDS             complement(237..1088)
                     /locus_tag="PKN22_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNS42582.1"
                     /translation="MRLKIAYNFFIVLCAIGMGSCTKQNPVPSLENTSSNKEVSQEIV
                     PIESTTKMVEITGGKYMPFYSADDSLVFVASFLMDETPVTNQDFLEFVKANPAWRKSN
                     IKKIYTDSTYLRDWVSDTVLPKGSNPNAPVCFVSWFAAKAYAKAVGKRLPTMDEWEYV
                     AMADETEPNARGKSSYSNNILNSYLIKDRQLKAVKQSKPNYWGVYNMFDLVWEWTDDF
                     NSIMVSGESRDGGNGDKNLVCAAGATNATDVMNYSAFMRFGLRTSLKANYTVANLGFR
                     CVKEIEK"
     gene            complement(1119..2147)
                     /gene="nirK"
                     /locus_tag="PKN22_07475"
                     /pseudo
     CDS             complement(1119..2147)
                     /gene="nirK"
                     /locus_tag="PKN22_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
     gene            complement(2185..>3459)
                     /locus_tag="PKN22_07480"
     CDS             complement(2185..>3459)
                     /locus_tag="PKN22_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42583.1"
                     /translation="QKRNNSFHTIRILSGQIKPRAELRYGNFSPFEKGDNPAALISQR
                     TRLNLFYEYKDIFSVKISPQMVNIWGQDGNTQGLGITNGFSLFEGWARINSSKSTHFK
                     IGRQIISLDDERFYGALDWAQGGRTHDALSFHWKHKIVELSTFLAYNQNYKQLYGNNI
                     NNPVGNLYSPIGATPYKWMQTLWAKFNITDKDAISLLATNLGFQTMLTANDTARNYNN
                     QTYGINYFHTGKKWNINASGYYQMGQNAGGRKISAYLAAVGVSYKIANKWKIGIGSEL
                     VSGNKVGVAPATISNAFTSYFVTGHKFYGHMDYYYAGNGHKEAGLSDNFLTLGFIPSQ
                     KWDLFLNMHQFIAPYGVNNGIENLSNNLGQEFDLGFNIKINKFIKINGGYSLYITTPT
                     VAFLKNKTNTRMDQHWGWLTLDITPVFLNLKH"
BASE COUNT         1035 a          664 c          548 g         1212 t
ORIGIN      
        1 gtcagaaaca agtatcggac aagcagattt acaagttgta taaatcataa caacaactag
       61 tgtttttcca cgtaaatcag ctagctttaa atcttttcca ttttgatctt gccaattact
      121 ggttaaatta aaaattgaat cagaaggtaa ttcttcaaca actttaggtt gttccttaca
      181 ggatacaaaa aacaccaatg caagtaggaa tacaatatac tttttcataa cactttttat
      241 ttttcaattt ctttaacaca tctaaaacct aagtttgcaa ctgtgtagtt cgcttttaaa
      301 ctggttctta aaccaaaacg cataaaagca gagtagttca tcacatcggt tgcatttgtt
      361 gcacccgctg cacaaactaa atttttatcg ccatttccac catctctaga ttcaccagaa
      421 accatgatgg agttaaaatc atctgtccac tcccatacta ggtcaaacat gttgtaaaca
      481 ccccaatagt taggttttga ttgttttaca gcttttaatt ggcgatcttt gattaaataa
      541 gaatttaaaa tattgttgga ataggagctt tttccacgag catttggttc tgtttcatct
      601 gccatagcta catattccca ttcgtccata gttggtaatc gtttgcctac agcttttgca
      661 taagcctttg cagcaaacca tgagacaaaa cagacaggag cgttgggatt gctacctttt
      721 ggcagaacgg tatctgatac ccaatctctt aaataggtgc tgtctgtgta tattttttta
      781 atattgcttt ttctccaagc agggtttgcc tttacaaatt caagaaagtc ttgattggta
      841 acaggcgttt cgtccattaa aaaagaggct acaaatacca gtgaatcatc agcgctataa
      901 aaaggcatgt atttacctcc ggtaatttct accattttag ttgttgattc aattggtact
      961 atctcttggc taacttcttt attggatgaa gtgttttcaa gagatggtac gggattctgc
     1021 ttggtgcagg atcccatacc aattgcacat agtacgataa aaaagttata tgcaattttt
     1081 aatctcatga taatcaattt tatttattag tgagttcctt ttttgaaaac ttttgcattt
     1141 tcatctccaa caactttaat ttgagccaat gcccctttgt taaatgttct tgacaaagag
     1201 tggtcaacga tgttgtaaac tccaggaacc tcaactttaa actcaacaat tgcagaacca
     1261 ccagcaggga tgattgtagt ttgtacgttg tgagttactg tagaaccacc ttctaaataa
     1321 acgttgtcaa agatttctcc aattacgtgg aaagaagata ctaagtttgg tccaccgtta
     1381 ccaacaaata aacgaactgt ttcaccaact ttagcttcca atgcatttgg tccaaccatc
     1441 aatccttttt taccgttgaa caatacgtaa tcaggatttt cagcaacagc tttgtcgttg
     1501 tcaaattcta aagttccagt tgcacctgct tttgtataga attcaccttg catgatgtag
     1561 tactcacggt caacttttga taaaccacct gctggctcaa ccaaaattaa accatacata
     1621 ccgttaccaa tgtgcatagg tacaggaggt gcagcacagt ggtaaacata caatcctggg
     1681 ttcaacgctt taaatgtaaa aaccgcttct ttacccggtg cagcaaaagt tgcttcagca
     1741 ccaccacctg gtccgtttac agcgtgtaag tcaatgttgt gcggcattac actgtttgaa
     1801 gcatttttca agtgtaattc cacttcatcc ccttcacgta cacgaatgaa gcttcctgga
     1861 gattgattga atgtccaaaa tgtatatttt tggccatcag caatttcagc ttcgatttct
     1921 tctgttgtta aatgtacgat tactttttta ggagctctgt caccaattgg tgcaggtaca
     1981 ttaggagctg ctgttaattc taattcaaca ggtgatcctt ctgcaaccat atcaattgca
     2041 gctgttccac tttttggagc agatacatct cctccttcag ttttactttt acacgctaca
     2101 aaaagtaggg gtaaagccag tccaaataaa attgcttttt ttgtcatgat gatttgtttt
     2161 tattgtttaa gaatttatta ctaattaatg ttttaaattt aaaaaaacag gagttatatc
     2221 taaagttaac catccccaat gttggtccat tctggtattc gttttatttt taagaaaggc
     2281 aacagttgga gttgttatat ataatgaata cccgccattt atttttataa atttattaat
     2341 ctttatgtta aaacccaaat caaattcttg acccaagtta tttgataaat tttctatgcc
     2401 attattaact ccataaggcg ctataaattg atgcatgttt aaaaataaat cccatttttg
     2461 tgagggaata aaaccgagcg taagaaagtt gtctgacaat ccagcttctt tgtgaccatt
     2521 gcctgcataa taataatcca tgtgtccata aaatttatgt cctgtcacaa agtaagaggt
     2581 aaatgcattg cttatcgttg ccggagctac acctacttta tttccactca ctaattcact
     2641 tcctatccca atcttccatt tatttgctat tttgtatgaa acacctacag cagctaaata
     2701 ggcactaatc tttcttcctc ctgcattttg tcccatttga taataacctg atgcgttgat
     2761 attccatttc ttccctgtat gaaaataatt gataccatac gtttggttgt tatagtttct
     2821 tgctgtgtcg ttagcagtta gcattgtttg aaaacctaag ttagttgcca ataaactaat
     2881 tgcatctttg tctgtgatat taaacttagc ccataaagtt tgcatccatt tgtagggagt
     2941 tgctccaatt ggagaatata aattacctac aggattgtta atgttgttgc catacaattg
     3001 tttgtaattt tgattgtaag ctaaaaaagt acttaattcc acaattttat gtttccaatg
     3061 aaatgaaagt gcatcatgtg ttcttcctcc ttgtgcccag tccaaagctc cgtaaaaacg
     3121 ttcatcatct agtgaaataa tttgtcgacc aattttgaaa tgtgttgatt tacttgagtt
     3181 tattcttgcc catccttcaa ataatgaaaa tccatttgta attcctaaac cttgagtgtt
     3241 tccatcttgt ccccaaatat taaccatttg tggcgatatt tttactgaaa aaatgtcttt
     3301 atattcataa aacaaattta atcgtgtgcg ttgactaatt aatgcagctg gattatctcc
     3361 tttttcaaat ggagaaaaat ttccatatct taattcagcc ctaggtttta tttgtccact
     3421 taaaatgcga attgtatgaa acgaattatt tcttttttg
//
LOCUS       DAOQVQ010000233         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31152_length_3458_cov_2.940641, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000233 DAOQVQ010000000
VERSION     DAOQVQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31152_length_3458_cov_2.940641"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKN22_07485"
     CDS             complement(<1..284)
                     /locus_tag="PKN22_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42584.1"
                     /translation="MRNSILNTKALLLCGAMLVGSTVKAGNEDRVGQAGAAHLLINPW
                     ARSSALGDVGVSFTKGLEASFTNIAGLASTEKTQIKFNYTNWMGSMTGLS"
     gene            complement(313..>3458)
                     /locus_tag="PKN22_07490"
     CDS             complement(313..>3458)
                     /locus_tag="PKN22_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42585.1"
                     /translation="DYVGCDVSRGLGYCYNGTPNDVTNGSQIGYGQNPPAIGVDFFEG
                     PYQDPDNMDNPGPVWDATTQSWITPTVSDAILNKGIVYNGIGTGYGDGVIDNERFGMR
                     RFTYFTNAASYPYTDPSVANQYYNFMSGSWADGSNITFGGTGAGGSILTDYVFPGDSD
                     PLHWGTAGVDPGNSTPWTEASAGNAVGDRRFVQSAGPFTLVPGASNNITVGIVYGRAA
                     GGDLMASVDLMKTADTKAQALFDACFRIIDPPNAPKLQIQELENELILTLSNPTTSNN
                     YLEKYEEIDDINIPVTETDRKYRFEGYQIFQLVDDVASVADIHDLSKARLVAQCDIKN
                     GVKDLTNFTLNEQYGILEGKRMVTGADAGIQHTFRLTKDAFALGDDRLVNHKTYYYVA
                     VAYGYNQFKPYDQNDPTKLDGQKKPYISSRLSYDGTSIQSVPGTPHNPRPEAGGTFQL
                     AEYGTTPRITRLDGYGNGNRALELTTESRNTIINSGSLAKLKYDFGGGPVNVKVIDPL
                     NVVNGYFELEFNDYTPTSSIGSYNSADTASWVIRRYASEGGALIDTISSEHTIKVDNE
                     QIIPQWGVSVQIYQTKYTNTSTKSQEAYAMPISSSITFADSSKRWLMSITDNDLYQPT
                     NWIRSGTAAAGTADPTAPAWLNPANYGDELAAGGDPDQHYEKVLNGGIAPQRVTGYNV
                     EFMPMAYMPNVTPSYSRELSSISYLPSVDIVITSDKSKWTRVPVIEMGRDNTMTFGGA
                     SAGQMRKSQSVDKNGNPDGTGTGMGWFPGYAIDLETGNRLYMAFGENSYMTQDNGRDM
                     IWNPSSRMVDNNGNFVMGGVHPVWIYGSEYQKINGVPNHLRDMPAYDPSVAVSNNFLE
                     EKLREIEAGTNVTNNTRFVYGNLAWIAYPMLTPGQTLLSTDVTIKLRVNKEYKNYVAT
                     NANAGKPKYSWNMDEIATKTGNRQALSEVLEMINIVPNPYLAYSEYEKSRLDTRVKIV
                     NLPEVCTVTIYSSNGKLIRQYKKDSPVTSIDWDLNNHSRIPVASGMYLIHVKVPDVGE
                     RVLKAFIGVRQVDLQGI"
BASE COUNT         1062 a          714 c          573 g         1109 t
ORIGIN      
        1 aatgataatc cagtcataga tcccatccag ttggtatagt taaatttgat ttgagttttc
       61 tctgtgctag ccaatccagc aatgtttgta aaagatgctt ccaatccttt agtaaaagaa
      121 acaccaacat cgccaagagc agaacttcta gcccaagggt ttatcaataa gtgagcggca
      181 ccagcctgac caaccctgtc ttcgttacca gcttttactg tagagccaac taacatggct
      241 ccacaaagta aaagtgcttt agtatttaaa attgaatttc tcatgttctt caattaatct
      301 gattaatatt gattaaatac cttgtaagtc aacttgacgt acaccaataa atgctttcaa
      361 tacgcgttca cctacatctg gtactttaac gtgtatcaag tacataccag aagcaactgg
      421 aatacgtgaa tggttattca aatcccaatc aatagatgta accggactat ctttcttgta
      481 ttggcgaatc aatttaccat ttgatgaata gatagtaact gtacatactt caggtaagtt
      541 tacaattttc acacgagtat ccaaacggct tttttcgtac tctgaatatg ccaagtaagg
      601 attaggaacg atattaatca tttctaacac ttcagataaa gcttgtctgt taccagtttt
      661 tgttgcaatc tcatccatgt tccaagaata tttaggttta ccagcattag cgtttgttgc
      721 tacgtagttt ttatattctt tattaacacg taatttaatt gtaacatcag tactcaacaa
      781 tgtttgacct ggtgttaaca ttggataagc aatccaagct aagttaccat aaacaaaacg
      841 agtgttgtta gttacgttag taccagcttc aatttctctt aatttttctt ccaagaaatt
      901 attagaaacg gctactgatg ggtcataagc tggcatgtct cttaagtggt ttggcacacc
      961 attgattttt tggtattcag aaccataaat ccaaactgga tgaacacctc ccattacgaa
     1021 gttaccattg ttatccacca ttcttgaaga tgggttccaa atcatgtcac gaccgttatc
     1081 ttgtgtcatg tatgagtttt caccaaacgc catgtataaa cggttacctg tttccaagtc
     1141 aattgcataa cctgggaacc atcccatacc agttcctgtt ccatcagggt ttccgttttt
     1201 atcaacactt tggctttttc tcatttgacc agcacttgca ccaccaaaag tcattgtgtt
     1261 atctctaccc atttcaatta caggtacacg tgtccactta ctcttatcag atgtgataac
     1321 aatatcaaca cttggtaagt aagaaattga tgacaattct ctagagtaac ttggcgttac
     1381 gttaggcata taagccatag gcataaactc aacgttgtaa ccagtaactc tttgaggcgc
     1441 aataccaccg tttaatactt tttcatagtg ttgatctgga tctccacctg cagccaactc
     1501 atcaccgtaa tttgcagggt ttaaccatgc tggagctgtt ggatctgccg ttccagctgc
     1561 tgcagtacct gaacgaatcc agtttgttgg ttgatacaaa tcattatcag taatagacat
     1621 taaccaacgt ttagaactat cggcaaaagt tattgaagaa gaaataggca ttgcatacgc
     1681 ttcttgagat tttgttgaag tattggtgta ttttgtttga taaatttgaa cagacacacc
     1741 ccattgagga ataatttgtt cattatctac tttaatcgta tgttcacttg aaatagtgtc
     1801 aatcaaagca ccaccttcac ttgcatatct tctaattacc caacttgcag tatctgcaga
     1861 gttatatgaa ccaatgctac ttgtaggagt ataatcattg aactctaatt caaagtatcc
     1921 atttactaca tttaacgggt caattacttt aacgttaact ggtccaccac cgaaatcata
     1981 tttcaactta gctaatgaac cactgttaat aattgtgttt ctagattctg tagttaattc
     2041 caacgctcta ttaccatttc cataaccatc taatctagta atacgtggag tagtaccata
     2101 ttcagctaat tggaatgtac cacctgcttc tggtctagga ttatgaggtg tacctggtac
     2161 agattgaata gaagtaccat cataagacaa acgagaagag atatatggtt ttttctgacc
     2221 atctaatttt gttgggtcat tttggtcata cggtttgaat tggttataac cataagctac
     2281 agcaacataa taatatgttt tatggttaac caaacgatca tcacctaaag caaatgcatc
     2341 ttttgttaat ctgaaagtat gttggatacc tgcatcagca cctgtaacca tacgtttacc
     2401 ttccaaaata ccatattgct cgttcaatgt aaagttagtt aaatccttaa caccattttt
     2461 aatatcgcac tgtgcaacaa gacgtgcttt agataaatcg tgaatatcag ctactgatgc
     2521 aacatcatcc accaattgga aaatttggta accttcaaaa cggtattttc tatctgtttc
     2581 agttactgga atattgatat catcaatttc ttcatatttc tctaaatagt tattagatgt
     2641 tgttgggttt gacaatgtca aaatcaattc attttccaac tcttggattt gcaattttgg
     2701 agcatttggt ggatcaatga tacggaaaca tgcatcaaac aatgcttgtg cttttgtatc
     2761 tgctgttttc attaaatcaa ctgaagccat taaatcacca cctgcagcac gaccataaac
     2821 gataccaact gtaatgttat tacttgcacc tggaaccaat gtaaatggac cagctgactg
     2881 cacaaaacgt ctatcaccaa cagcattacc tgcagatgct tctgtccatg gagttgagtt
     2941 tcctggatca acacctgctg ttccccaatg caatgggtca gagtcacctg ggaatacata
     3001 gtcagttaat attgaacctc cagcacctgt tccaccaaat gtaatgttag aaccatcagc
     3061 ccagctaccc gacataaagt tataatattg gttagctaca gaagggtctg tatatggata
     3121 agaagcggca tttgtaaagt aagtgaatcg acgcatacca aaacgctcat tgtcaataac
     3181 tccatcacca taaccagtac ctatcccatt ataaacaata cctttattta aaattgcatc
     3241 actcactgtt ggagtaatcc aagattgtgt agttgcatcc caaactggac ctgggttatc
     3301 catattatct ggatcttggt aagggccttc aaagaagtca acaccaattg caggcggatt
     3361 ttgaccatat ccaatttgtg aaccgttggt tacgtcattt ggtgtaccat tataacagta
     3421 acctaaacca cgagaaacgt cacaaccaac atagtcgt
//
LOCUS       DAOQVQ010000234         3457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31157_length_3457_cov_4.531158, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000234 DAOQVQ010000000
VERSION     DAOQVQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31157_length_3457_cov_4.531158"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..744
                     /locus_tag="PKN22_07495"
     CDS             73..744
                     /locus_tag="PKN22_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNS42586.1"
                     /translation="MELIHSFLDFFLHLDEHLHSMITNYGSWIYIILFIIIFVETGLV
                     VMPFLPGDSLLFTAGAFCAGVVNEAGNVANLNLWLVLVLLLIAAILGDATNYMIGKRL
                     GLKMLKWQVGGKQLVKQKYIDQTHDFFEKHGPKTIIIARFVPIVRTFAPFVAGIGEMT
                     YAKFFRFNIIGGTLWIVSLTLLGYFFGNLEFVKKNFEIVVFGIIGISLLPMIFEIIKA
                     KTAKK"
     gene            744..1487
                     /locus_tag="PKN22_07500"
     CDS             744..1487
                     /locus_tag="PKN22_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNS42587.1"
                     /translation="MAHIQKYGLIGKKLGHSFSKQFFTDYFSEKGSAATYENLEFETN
                     EELATFFQSTVFEYHGLNVTIPYKEAVLPFLGELSDEVQQIKAVNLIQVNNGKLKGYN
                     TDAYGFQQSIKPFLTNQHERALILGTGGASKAVHYVLKQLGIDCIFISRNPNGNKQFA
                     YNQINNHMLNACKLIVNCTPVGMYPHVNDCIDLPFDCLTPQHLVVDLIYNPTKTQLLQ
                     KSEDAGAQILNGDSMLKQQALKAIEILGL"
     gene            1597..2247
                     /gene="upp"
                     /locus_tag="PKN22_07505"
     CDS             1597..2247
                     /gene="upp"
                     /locus_tag="PKN22_07505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNS42588.1"
                     /translation="MMIHNLSKTNSIFNTFLAEMRDTSIQNDAMRFRRNLERIAEIMA
                     YEVSKQLEYETTNVTTPLGEIETAQLKEQPVLATILRAGLGMHQGVLNYFDKAQSAFI
                     SAYRKHSSAEDYEIYVEYIAAPVIDDKIVIITDPMLATGNSMVTVYKALLQRGKPKKV
                     YILSAVAHADAIDFVRKHLPANTEIWVGAIDAELTAQSYIVPGLGDAGDLAYGLKV"
     gene            2250..2732
                     /locus_tag="PKN22_07510"
     CDS             2250..2732
                     /locus_tag="PKN22_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42589.1"
                     /translation="MKWVALSTVFFSAMIKFMVSPLLGPLLELNYWETYFANLLGAFV
                     SMVVFFFAADFFNKRSMQRRLKKAKQEALEGKPIKRKPVFTRRNKAIIRIKNKVGIVT
                     FCFFAPFFLSIPIGSIIVAKFYGKKKKTFPLMILGIIITNTLTVSIAYLLKYEAEYLF
                     "
     gene            2710..3414
                     /locus_tag="PKN22_07515"
     CDS             2710..3414
                     /locus_tag="PKN22_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNS42590.1"
                     /translation="MKQNIFFDLDKTLWDFETNSERALKYLFDSTQDKHQLPSFHKFL
                     QIYKEVNATLWVKYGKKKITKEELRTNRFKTTFFKLGLFNNEISQYFEEEYIRISPLQ
                     TTLFPNTIETLKMLQNDDYQMHIITNGFKEVQYIKLENSGLLPFFDKIICSEEVGVNK
                     PNIEIFQHALQLANAKAEKSVMIGDDWEVDVMGAERAGMHGVHFDAEKKYQRSKVENR
                     IHNLNELPDLLPWLLK"
BASE COUNT         1127 a          607 c          623 g         1100 t
ORIGIN      
        1 atcactggtg gcattatagc atggaaagaa gaagtagata attcattaaa tctttaaatt
       61 tcacacaact aaatggaatt aattcactcg tttttagact tttttcttca cttagacgaa
      121 cacctccatt caatgataac caactatggg agttggattt acattatttt gttcatcatt
      181 atttttgtgg aaacaggatt ggttgtcatg ccatttttac caggcgattc gctcttgttt
      241 actgcgggtg cattttgtgc tggtgtagta aatgaagcag gcaatgtggc aaatttaaat
      301 ctttggttgg tgcttgtttt gctgttaatt gctgctattt taggcgatgc aaccaattat
      361 atgataggta aacgactggg actaaaaatg ctaaaatggc aagtgggtgg caagcaattg
      421 gtaaaacaaa aatatattga tcaaacccat gatttttttg aaaaacatgg accaaaaacc
      481 atcatcatag ctcgttttgt acctattgta cgcacatttg ccccatttgt tgctggtatt
      541 ggcgaaatga cctatgcaaa attctttcgt ttcaacatca tcggtggcac tttgtggatt
      601 gtgtcgttaa ccttgcttgg atactttttt ggcaacctag aatttgttaa aaagaacttt
      661 gaaattgtag tatttggaat catcggtatc tcgttgcttc ctatgatttt tgaaatcatc
      721 aaagccaaaa ctgcgaaaaa ataatggcac acattcaaaa atatggattg attggcaaaa
      781 aactcggtca ttctttttct aaacagtttt ttaccgacta tttttcagaa aaaggtagcg
      841 ccgcaactta tgagaattta gaatttgaaa ccaatgaaga attggcaact ttctttcaat
      901 ctactgtttt tgaataccac ggattaaacg ttaccattcc ctacaaagaa gctgttcttc
      961 cgtttttggg cgaactctct gatgaggtgc aacaaataaa agccgtcaat ttaattcaag
     1021 taaacaacgg gaaattaaaa ggctataaca ccgatgctta tggttttcag caatccataa
     1081 aaccgttttt aaccaatcag catgagcgtg ctctaatttt aggtacaggt ggtgcttcca
     1141 aagcggttca ctatgtttta aaacaattag gaattgattg catttttatc tcgcgcaatc
     1201 ccaatggaaa taagcaattt gcttacaatc aaatcaataa tcacatgcta aatgcctgca
     1261 aattaattgt caattgcaca cctgttggta tgtaccccca tgtgaacgat tgcatcgatt
     1321 tgccatttga ttgtttaaca ccacagcatt tggtggtaga tttaatttac aatcccacca
     1381 aaacacaact cttgcaaaaa agtgaggatg ctggagctca aatcctcaat ggcgattcca
     1441 tgttgaaaca acaagcatta aaagccattg aaattcttgg tctgtaaccc cttaaagcta
     1501 ttggtggtta atatttccta aaatttaaag gttgacataa aaatatgtgg caattatcag
     1561 attttaattg taaattagcc catatatgca caactaatta tgattcacaa tttatcaaaa
     1621 actaattcca tcttcaacac atttctggca gaaatgagag acacaagtat tcaaaacgat
     1681 gccatgcgct tccgtcgaaa tttagaacgc attgcagaaa ttatggctta tgaagtgtca
     1741 aagcagttgg agtatgaaac gactaatgtt acaactccct tgggcgaaat agaaacagct
     1801 caattgaaag aacaaccagt tttggcaacc atcctacgtg caggtttagg catgcatcaa
     1861 ggtgtattaa attattttga caaggcgcaa agtgctttta tttctgccta tcgcaagcat
     1921 tcatctgccg aagattatga aatttatgtg gaatacattg ccgctcctgt aattgatgat
     1981 aaaattgtaa tcatcaccga ccctatgttg gcaacaggta actcaatggt tacggtttac
     2041 aaagcccttt tgcagagagg aaaacctaaa aaagtgtata tcctttcggc agttgctcat
     2101 gcagatgcca ttgattttgt acgtaaacat ttacctgcca ataccgaaat ttgggtaggt
     2161 gcaattgatg cagaattaac ggctcaatcc tatattgtac ctggacttgg cgatgcaggt
     2221 gatttagctt atggtttaaa agtgtaagca tgaaatgggt agctctcagc acggtttttt
     2281 tctctgctat gatcaaattc atggtgagtc cgctgttggg gccattgctc gaactaaact
     2341 attgggaaac ttatttcgca aatttattag gtgcttttgt ttctatggtt gttttctttt
     2401 ttgctgccga tttttttaat aaacgaagca tgcaaagacg tttaaaaaaa gcaaaacaag
     2461 aggcattgga aggtaagccc attaaacgca aaccagtgtt tactcgacga aataaagcca
     2521 tcattcgtat aaaaaataaa gtaggtattg taacgttttg tttctttgca ccgtttttcc
     2581 tttccatccc cattggttcc atcattgtgg ccaaatttta tggaaagaaa aaaaagacat
     2641 ttcctttaat gattttagga ataattatta ccaacacatt gacggtgagt atagcatact
     2701 tattaaaata tgaagcagaa tatctttttt gatttagata aaacattgtg ggattttgaa
     2761 accaattctg aacgtgcatt aaaatatctt tttgactcca cacaagacaa gcatcaattg
     2821 ccgagttttc ataaattttt gcaaatttat aaggaagtaa atgctacgtt gtgggttaaa
     2881 tacggcaaaa agaaaatcac caaagaagag ttacgcacca atcgttttaa aacaactttt
     2941 tttaaattag gcttgtttaa taatgagatt agccaatatt ttgaagaaga atacatacgt
     3001 atttcaccct tgcaaacaac gttgtttcca aataccattg aaaccctaaa aatgttgcaa
     3061 aacgatgatt atcaaatgca catcatcacc aacggattta aagaagtaca atacatcaaa
     3121 ttggaaaaca gtggattatt accttttttt gataaaataa tttgttcaga agaagtaggt
     3181 gttaacaaac ctaatattga aatttttcaa catgcgcttc aacttgccaa tgccaaagct
     3241 gaaaaatcgg taatgattgg cgatgattgg gaagtggatg tgatgggtgc cgaacgtgct
     3301 ggcatgcatg gcgttcattt tgatgctgaa aaaaaatacc aacgcagtaa agttgaaaat
     3361 agaatacaca atttaaacga actacctgat ttattgccgt ggctgttaaa ataaattact
     3421 caaactcaac ttccactttc ttcttctctt cgccgtt
//
LOCUS       DAOQVQ010000235         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31286_length_3448_cov_3.185971, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000235 DAOQVQ010000000
VERSION     DAOQVQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31286_length_3448_cov_3.185971"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="gldL"
                     /locus_tag="PKN22_07520"
     CDS             complement(<1..596)
                     /gene="gldL"
                     /locus_tag="PKN22_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNS42591.1"
                     /translation="MKPGSKSWKKFMAKLYGFGAAVVIVGALFKIQHYPGASLMLVLG
                     LSTEALIFIFSAFEPIHEDPKWELVYPELALGDSEELDKDALPVASGRGGRGGSGTGV
                     TEHLDNLLAEAKIDGELIGRLGDGMRRLGDNAAQLSSVTTAAAATDSYVNSLQAASQK
                     VSSLSEAYERASVSISGLTSTTAEGESFGEQMQKVSKN"
     gene            complement(656..2203)
                     /locus_tag="PKN22_07525"
     CDS             complement(656..2203)
                     /locus_tag="PKN22_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNS42592.1"
                     /translation="MKKLLLIGFVGAIFASCQTRTGHLTGTLGRPVYHPQIPLGMVYI
                     KAGSYQMGEDDQDVPFLHQTRAKTASVQAFYMDQTEISNNEYRQFVDWVRDSIAREKI
                     YLSDLMEDEDASKFINYSELYFDEGALEYVDFEPSDRDINSSIFSLNWDRKFSYEDPM
                     LVPILADMYYPQPQRFYKRREIDTRKLMFRYYWIDLVEAARRGRIDIVQDGYDFQGTK
                     QADNHRTLNTGPHPFTQEPQGQDLDMGGFNKKGQNNAIRGHENRQRFIIDEIINVYPD
                     TLCWVRDFTYSFHDPMTNMYFWHPAYDNYPVVGVTWQQAKAFSVWRTQLLNSWLAEMG
                     ELFVNDFRLPTETEWERAARGDLNMSQYPWGGPYVRNESGCFLGNFKPMRGRYFEDGG
                     FHTVKVYSYNPNGFGLYCMAGNVSEWCESAYDESMYEFSHDLNTEYRYDAMDWDPPSM
                     KRKVTRGGSWKDIGYYIRTATRSYEYQDTAKSFLGFRNVATHLGRGGSDFSKEGGEEI
                     RSDINLR"
     gene            complement(2315..3253)
                     /locus_tag="PKN22_07530"
     CDS             complement(2315..3253)
                     /locus_tag="PKN22_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS42593.1"
                     /translation="MKKRLLVISAFLFTFSGGVMAQQDKLITHFIYDKMSVNPGATGF
                     DRDLCATLMYRNQWDKVNGAPNSVVFNLEANMRRWFPGGLGLSFYHDAIGFNRQNNLL
                     LNYSYPLDIGSAGTLGIGVGIGMINFGQNPTWVPPTTQNDISLPVGFNSTNLDLNFGL
                     YFKGNADWYAGISSTHLSESLLKKKINNVDHAYQTKRHYYIMGGKTFNNIGKNDKIDL
                     QTLIRTEFTKFSADINARYIWRDMLYGGITYRTIDAVGLMVGYMPINGLVISYSYDIT
                     TNKLASVSRGSHEIALRYCYKLPPVPIQTSRHPRWL"
BASE COUNT         1072 a          709 c          526 g         1141 t
ORIGIN      
        1 aagtttttag aaactttttg catttgctca ccaaatgatt caccttcagc agttgttgaa
       61 gttaaaccag agatagaaac agatgctctt tcgtaagctt cagataaaga agatactttt
      121 tgagatgctg cttgtaagct atttacataa gaatcagttg ctgctgcagc tgtagttaca
      181 gaagacaatt gtgctgcatt gtcacctaaa cgacgcatac catcacctaa tcttccaatt
      241 aattcaccat caattttagc ttcagccaac aaattatcta agtgttctgt aacaccagtt
      301 ccagaaccac ctcttcctcc tctaccacta gcaactggta aagcatcttt gtctaattct
      361 tctgaatctc ctaaagctaa ttctgggtaa actaattccc acttcggatc ttcgtgaatt
      421 ggttcaaatg cagagaaaat aaagatcaat gcctcagtac tcaaacccaa tacaagcatc
      481 aacgacgccc ctgggtagtg ctgaatttta aataacgctc ccacgattac aactgctgca
      541 ccaaaaccgt acaacttagc cataaacttt ttccaacttt tacttcctgg tttcataatt
      601 cttctagttt tttttggttt ttaattaatt aattttagat tgtttttgtt tattcttagc
      661 gaaggtttat gtcgctacga atttcttcgc ctccctcttt cgagaagtca cttcctcctc
      721 ttcctaagtg tgttgccacg tttcggaatc ctaagaatga cttagcagta tcttggtact
      781 cgtaactacg agttgcagtt ctaatataat atcctatatc tttccatgaa ccaccacgtg
      841 taactttacg tttcattgaa ggtggatccc agtccattgc atcatatctg tattctgtat
      901 tcaggtcatg tgagaattcg tacattgatt catcgtacgc tgattcacac cattctgaaa
      961 cgtttccagc catacagtac aatccaaatc cgttagggtt atacgaataa acttttactg
     1021 tgtggaaacc accatcttcg aagtagcgac ctctcattgg tttaaagttt cctaagaaac
     1081 atcctgattc gttacgaaca tatggtccac cccaagggta ttgagacata ttcaagtcac
     1141 ctcttgcagc tctttcccac tcagtttctg ttggtaaacg gaaatcattt acaaacaatt
     1201 cacccatctc tgccaaccag ctgttcaaca attgcgttct ccaaactgag aaagcttttg
     1261 cttgttgcca agttacacct acaactgggt aattatcata agcaggatgc cagaaataca
     1321 tatttgtcat tggatcgtgg aacgagtaag tgaaatcacg aacccaacat aatgtatctg
     1381 gataaacatt tattatttca tcaatgataa aacgttgacg gttttcgtgt ccacgaatag
     1441 cattgttttg tcctttttta ttgaacccac ccatatccaa atcttgacct tgaggttctt
     1501 gtgtaaacgg atgtggccct gtgttcaacg tacggtgatt atctgcttgt ttagtgcctt
     1561 ggaaatcata accatcttgc acgatatcaa ttcgtcctct tctagcagct tctactaaat
     1621 caatccaata ataacggaac attaattttc ttgtatcgat ttcacgacgt ttatagaaac
     1681 gttgtggttg tggataatac atatccgcta aaatcggcac gagcataggg tcttcataag
     1741 agaactttct atcccaattc aagctaaaaa tactactatt gatatctcta tctgaaggtt
     1801 caaaatcaac atattccaat gcaccttcat caaaatacaa ttcagaatag ttgatgaatt
     1861 ttgacgcatc ttcatcttcc atcaaatctg ataagtaaat tttttctcta gcaatagagt
     1921 cacgcaccca atctacgaat tggcgatact cattattgga gatttccgtt tggtccatat
     1981 aaaatgcttg gacagacgca gttttagcac gcgtttggtg taaaaacggt acatcttgat
     2041 cgtcttcacc catttgatag gaaccagctt taatgtaaac catcccaagt ggaatttgtg
     2101 gatgataaac cggtcttcca agtgttccag ttaagtgccc agttcttgtc tgacaagaag
     2161 cgaaaattgc accaacaaaa ccaattaaca aaagtttttt cattctacct cgttttaatt
     2221 tgccgaatca aaaattacgt actacaggat caattctttt ttgatgtaac ggcattgcct
     2281 gaaaaaggtt acaaatatat tttatttttt ttaattacaa ccatcttggg tgacgcgaag
     2341 tttggatcgg caccggtggt aatttataac agtatctcaa tgcgatttca tgtgaacctc
     2401 ttgaaacact agctaattta tttgttgtaa tatcataaga ataactgatt accaagccat
     2461 tgattggcat ataacctacc atcaaaccaa ctgcatcaat tgttctatag gtaataccac
     2521 catacaacat atctctccaa atataacgtg cgttgatatc tgcagaaaat tttgtaaatt
     2581 cagtacgaat taatgtttgc aaatcaattt tatcattttt tccgatgtta ttaaacgtct
     2641 taccccccat gatgtaatag tgacgttttg tttgataagc gtgatcaacg ttattaattt
     2701 tctttttcaa taacgactcg ctcaagtgtg tagatgaaat acctgcatac caatctgcat
     2761 ttcctttgaa gtataaaccg aagtttaaat ccaagtttgt tgaattaaat ccaacaggca
     2821 aggaaatatc attttgtgtt gttggaggaa cccatgttgg attttgacca aaattaatca
     2881 taccaatacc aacaccaatt cctaaagtac cagcacttcc gatatccaaa ggataagaat
     2941 agtttaacaa caagttgttt tgtctgttaa aaccaattgc atcgtgataa aaagacaaac
     3001 ctagtcctcc aggaaaccat cttctcatgt tggcttctaa attgaaaaca acagagtttg
     3061 gcgctccatt tactttatcc cattgatttc tgtacatcaa cgttgcgcac aaatctctat
     3121 cgaaacctgt agcaccagga tttacactca tcttatcata aataaaatgg gtaattaact
     3181 tatcctgttg agccattact ccacctgaaa aagtgaataa aaaagctgag ataactagta
     3241 atcttttttt catataagta ttatttttag gcaatatcat tcaatattta tccaaaatta
     3301 gtaaacaagt tggcgactaa agtaatgata tttttttatt tattcagatt tttttaacaa
     3361 aatcgtaaaa aaaatatttt tccatttttt tagaaattaa attcaagtta taaatgcaac
     3421 ttttagattt tacgtcaaaa taacttca
//
LOCUS       DAOQVQ010000236         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31471_length_3433_cov_3.835998, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000236 DAOQVQ010000000
VERSION     DAOQVQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31471_length_3433_cov_3.835998"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKN22_07535"
     CDS             complement(<1..1033)
                     /locus_tag="PKN22_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNS42594.1"
                     /translation="MKKTLLLISSIFIGYSTFAQTQRCATVEYMNYRENLQNGYIQAT
                     QSVFNTAKKSNQQKSDETYIIPVVVHVVYKKAQENIADSVILDQIRVLNEDYNRLNAD
                     SVNLRNEFLPYVGNAKIEFRLAEFDPNGNPTTGITRTQTTLNSFLEMGSGLAEKVKST
                     ADGGIDPWNQSRYLNIWVCNMALTWGTTEIPMVLGYATPPANLPNWPAGSTGNMSDGV
                     VIQYQVFGTNNPTPLSGGTPYVVKGRTVTHEVGHYLGLRHIWGDGDCTQEDGIDDTPN
                     ADAQSQSDCNKTKNTCVDAIDGEDLPDLVENFMDYSAEDCQNTFTKGQVELMRAVIEN
                     NRWDLVNDND"
     gene            complement(1094..2284)
                     /locus_tag="PKN22_07540"
     CDS             complement(1094..2284)
                     /locus_tag="PKN22_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNS42595.1"
                     /translation="MKKIVLSIFSFVLIASINAQTTTNAPDSEYKFTKIAHLDATPVQ
                     SQGNTGTCWSFSALSFFESELIRKGNKNPDVLSEMFIVRKAYESKADKYIRMDGKTNF
                     SQGGAFHDIPYVVKRYGIVPQEVYSGLINGAKKYNHDELFAVLEGIVKGVNNGSKPIS
                     TSWKIAFNSILDAYLGQDVKEFEWKGKKYTPQSYAQAIGLNMDDYISLTSFTNHEMNK
                     PCMLEIQDNWAWGSSYNVSLEDLNNAAIYALKNGYTLAWGSDVSERGFSFSNGLGIVP
                     EDPETIKVKGRDNSNFSDGGAERTSDAFKTPVKELTITQELRQEGYDNKTTTDDHGMH
                     IVGLYKDQNGTNYYLVKNSWGTGNYPQGYLYVSENFFKYKTINIYLHKDGLSKEMKKK
                     LNIQ"
     gene            complement(2407..>3433)
                     /locus_tag="PKN22_07545"
     CDS             complement(2407..>3433)
                     /locus_tag="PKN22_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNS42596.1"
                     /translation="MFIAKLDANGNYLWAKSAGESSLDFGFSIAVDNQGNIYVTGRFR
                     GSVAFGSTTLATYGSLQIFITKLDTNGNFLWAKQAGDWNGNATGCSIAVDNQGNVFVS
                     GYFSFLATFGSTTFTSNNSNDDIFITKLDANGNFLWAKQAGGNNLENGKGIAVDSQGN
                     SYLAGYFEDIAAFGSATLTSNGNQDIYITKLDPNGNFIWIKQAGGSYKDICRGIAVDN
                     QGNSYVTGVIQDTVTFGSTTLTGNGSLNIFVAKINNSPLSVPTFESFTNILIYPNPAT
                     QQITVESSNALKVIELQDMSGRLITKKNVAGEKTNINVSNLNKGVYFVKVVDNQNKVK
                     VVKVAKE"
BASE COUNT          987 a          680 c          573 g         1193 t
ORIGIN      
        1 cgtcgttgtc atttaccaag tcccaacggt tattttcaat tacagcacgc attagctcaa
       61 cttgcccttt tgtaaacgta ttttggcaat cttcggcaga ataatccata aaattttcaa
      121 ctaaatcagg caagtcttca ccgtcaatag cgtccacaca tgtgtttttg gttttgttac
      181 aatctgattg tgattgagca tctgcatttg gagtatcatc aattccatct tcttgtgtgc
      241 aatcaccatc accccaaata tgacgcaatc ctaagtaatg acctacttca tgagtaacag
      301 ttctaccttt aacaacatac ggagtacctc cagaaagtgg agtaggattg ttggttccaa
      361 atacttggta ttgaatcaca acaccatcgc tcatattgcc agtacttcca gctggccagt
      421 taggtaaatt agcaggtggt gttgcataac ctaataccat aggtatttcg gttgtacccc
      481 aggtcaaagc catgttgcat acccagatat tcaaataacg agattggttc catgggtcga
      541 tacctccatc agctgtagat tttactttct ctgccaatcc agaacccatt tctaaaaatg
      601 aatttaaggt tgtttgagtt cgtgtaattc cagttgttgg attgccattc gggtcaaatt
      661 ctgctaaacg aaattcaatt tttgcattgc caacataggg taagaattca ttgcgcaagt
      721 tgacagaatc cgcattcaaa cggttgtaat cttcgttcaa cacgcgaatt tgatctaaaa
      781 ttacagaatc ggcaatgttt tcttgcgctt tcttgtaaac tacatgcaca actacaggga
      841 tgatgtaagt ttcatccgat ttttgttgat ttgatttttt tgccgtgtta aataccgatt
      901 gtgtagcttg aatgtacccg ttttggaggt tttcacggta attcatgtat tcaactgtag
      961 cgcatctttg cgtttgagca aatgttgaat agccaataaa aattgaactg attaaaagta
     1021 aagttttctt catagaaata gtttttaaaa aagagccata atagtagtga cgctaaaatg
     1081 gctcttatta agattattga atatttaatt ttttcttcat ctctttcgac aaaccgtctt
     1141 tgtgcaagta gatgttgatg gttttgtatt taaagaagtt ttcagacaca tacaaataac
     1201 cttgcggata gtttcctgtt ccccaagagt ttttcaccaa gtaataattg gttccgtttt
     1261 ggtctttata caacccaacg atgtgcattc catggtcgtc tgttgtagtt ttgttatcat
     1321 acccttcttg acgtaattct tgcgtgatgg ttaactcttt tacaggagtt ttaaacgcat
     1381 ccgatgttct ttctgcacca ccatctgaaa aatttgaatt gtctctacct tttactttaa
     1441 tcgtttcagg atcttctggc acaattccca aaccgttaga aaaagagaat cctctttcag
     1501 aaacatccga tccccaagca agtgtgtagc catttttcaa tgcgtaaata gccgcattgt
     1561 ttaaatcttc taaagacacg ttgtaagaag aaccccaagc ccagttgtct tgaatttcca
     1621 acatacaagg tttgttcatc tcatggttgg taaacgatgt taatgaaata taatcatcca
     1681 tgttcaaccc aattgcttgc gcatacgatt gtggtgtata ttttttaccc ttccactcaa
     1741 attctttaac atcttgccct aaataagcat ccaaaattga attgaaagct attttccaag
     1801 atgttgaaat aggcttactt ccgttgttaa cacctttaac gataccttct aataccgcaa
     1861 acaattcgtc gtggttgtat ttttttgcac cattaattaa tccgctataa acttcttgtg
     1921 gaacgattcc gtatctttta actacataag gaatatcgtg aaaagcacct ccttgagaga
     1981 agtttgtttt cccgtccata cgaatgtatt tatctgcctt gctttcataa gctttacgca
     2041 caataaacat ttctgaaagt acatctggat ttttattgcc tttgcgaatc aactctgatt
     2101 caaagaacga taaggcagaa aaactccaac aagttcctgt atttccttga ctttgaacag
     2161 gtgttgcgtc taaatgagca atttttgtaa atttatattc gctatctgga gcatttgttg
     2221 ttgtttgtgc atttatactt gcaataagta caaatgaaaa gatacttaat actatttttt
     2281 tcattttctt tattttttaa agagtgaaat ttatccacgc cccccaaaaa taaaaataaa
     2341 aatagacttt tgataattga agcgatttat tgtgctacaa aaatccaaat taacctttct
     2401 attttcttac tccttcgcca ctttcactac ttttactttg ttttggttat caaccacttt
     2461 tacaaaataa acgcctttgt tgaggtttga aacatttatg tttgttttct ctcctgcaac
     2521 attttttttg gtaattagtc gaccgctcat gtcttgtagt tctattactt ttagcgcatt
     2581 actactttca acggtaattt gctgtgtagc agggttgggg taaataagga tgttggtgaa
     2641 agattcaaaa gtaggaacag aaagaggtga gttgtttatt tttgcaacaa aaatgtttaa
     2701 agatccatta cccgttaaag tagtacttcc aaaagttaca gtgtcttgta taactccggt
     2761 tacatagcta ttcccttggt tatctactgc gatgcctcta catatgtctt tataacttcc
     2821 ccccgcttgt tttatccaaa taaagttgcc gttggggtct aatttcgtga tataaatatc
     2881 ttggtttcca ttacttgtta aagtcgcact tccaaaagca gcgatgtctt caaaatagcc
     2941 tgctagatag ctgtttcctt ggctatctac tgcgatgcct ttaccatttt caagattatt
     3001 gcccccggct tgtttagccc aaagaaaatt cccgttagca tctaatttgg ttatgaaaat
     3061 atcatcatta ctattattgc ttgtaaaggt cgtacttcca aatgttgcga ggaatgaaaa
     3121 atatcctgac acaaagacat ttccttggtt atctactgcg atactgcaac cagtagcatt
     3181 gccattccaa tcacctgctt gtttagccca aaggaagttt ccattggtgt ctaatttggt
     3241 tataaaaatt tgaagactac cataagttgc taaagtagta cttccaaaag ctacggaacc
     3301 tcgaaaccgt cccgttacat aaatgttgcc ttggttatct actgctatgc taaaaccaaa
     3361 atcaagacta ctttccccag ctgatttggc ccaaagatag ttgccattag catctaattt
     3421 ggcgatgaac ata
//
LOCUS       DAOQVQ010000237         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31665_length_3418_cov_3.581624, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000237 DAOQVQ010000000
VERSION     DAOQVQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31665_length_3418_cov_3.581624"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKN22_07550"
     CDS             <1..703
                     /locus_tag="PKN22_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNS42597.1"
                     /translation="FSCSENTNNLEDNQSPSLIEISALDFQKKIDELKEPQIVDVRTP
                     DEYAGGHIENAINIDWNGNNFNEEIQKLDKTQPILVYCLSGGRSGKAVHEMQQLGFNN
                     IIEMEGGMLDWRKNKLPETKNTSSTTKNTALTLEKYKALVNNPDKLVLVDFYAEWCGP
                     CKRMEPYINKFAESEKEKLEIIRIDADEYADLCTQLNVTALPTMKLYKNNAIVWENVG
                     FVSEADLLKVFQKNY"
     gene            869..2869
                     /gene="nosZ"
                     /locus_tag="PKN22_07555"
     CDS             869..2869
                     /gene="nosZ"
                     /locus_tag="PKN22_07555"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNS42598.1"
                     /translation="MRNLNLLKLSVLAVAAVAVGCKPKGVETVVSGNAAQKVYVAPGK
                     YDEFYNFVSGGFSGQMGVYGLPSGRLLKVIPIFSVNPENGYGYSEETKPMLETSYGFI
                     PWDDQHHLELSMTNGVHDGKWIFANANNTPRVARVDLKSFKTVEILEIPNSAGNHSSP
                     FATENTEYIVAGTRFSVPTDDSNGDVPIDSYKQNFKGVVSFISVDKETGDMNLSFQVE
                     APGVNFDLSRAGKGVSKDWFFFTCYNSEQANTLLEVNASQFDKDFIMALNWKKAEALV
                     KAGKGKKIKTKYAHNKLSEETHTATSTMKEDVTVLTIDDLKDAIYMIPCPKSPHGCDV
                     NPTGEYIIGSGKLAALIPVFSFDKIQKSISSKDFAGNYGGIPIIKYESALYGEVQKPG
                     LGPLHTEFDANGNAYTSMFLSSEVVKWNLKSLKIVDRQPTFYSTGHLMVCGGNTAKPY
                     GKYLVAYNKITKDRYLPTGPELTQSAQLFDISGEKMELLLDFPTIGEPHYAQAAPADL
                     ISKNQIKMYKIDHNKHPYVSMGEAKTKVVREGNKVHVYMTSIRSHFAPDNIEGIRVGD
                     EVYFHVTNLEQDWDLPHGFAIKGNQNAELLIMPGETSTLKWVPNKAGMFPMYCTDFCS
                     ALHQEMQGYVRVSPAGSNTPLIYSLNNKKDNASSPVKQGETK"
     gene            2922..>3418
                     /locus_tag="PKN22_07560"
     CDS             2922..>3418
                     /locus_tag="PKN22_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42599.1"
                     /translation="MNTNNLSQTSKLTFYLLIVCAIGFIVSIITPIWSIYLLAPQYPE
                     GLSMYLHANKLAGDVEIINGLNHYIGMKELHADDFWEFTYLPTILGVLSFITLLVAFL
                     KKKKFLYAFTIFYALFGIAFMVDFWWWEYQYGHNLDPSAAIIIPGMSYQPPLFGKKVL
                     LNFTA"
BASE COUNT         1197 a          560 c          623 g         1038 t
ORIGIN      
        1 gtttagctgt tctgaaaaca caaacaacct agaagataat caatctccat ctttaattga
       61 aatatctgct ttggattttc aaaagaaaat tgatgaatta aaagaacctc aaatagttga
      121 tgtcagaact cctgatgaat atgcaggtgg acatattgaa aacgctatca acatcgattg
      181 gaatggtaat aacttcaatg aagagattca gaaattagac aaaacccaac ctattttggt
      241 atattgttta agtggtggta gaagtggaaa agctgttcat gaaatgcaac aacttgggtt
      301 taacaacatt atagaaatgg aaggtggaat gttggattgg cgcaaaaaca aacttcctga
      361 aacaaaaaac acttccagca ctacgaaaaa tacagctttg acacttgaaa aatataaagc
      421 gttagttaac aatccagata aacttgtttt ggtagatttt tatgctgaat ggtgtggacc
      481 ttgcaagcgt atggaaccct acatcaataa atttgctgaa agcgaaaaag aaaaattaga
      541 aattattcgc attgatgccg atgaatatgc agacttatgc acacaattaa atgtgacggc
      601 acttccaaca atgaaattgt ataaaaacaa tgcaatcgtt tgggaaaacg taggttttgt
      661 tagcgaagca gacttgctaa aagtttttca aaagaattat taaaaagata gtctaaaaat
      721 taaaaatccc gagttagaaa taattcggga tttttttatc cttattttta aaaaacatga
      781 ctaaaatcat atttttaatt tatatttgag aataaatttg tatcgaataa atgtaaataa
      841 acgatttaat catttaaaac aaaatcaaat gagaaatttg aatctactta aattatcagt
      901 cttagctgtt gcagcagttg cagtaggctg taaacctaag ggagtagaaa cggtagtgag
      961 cggtaatgcg gctcaaaaag tttatgttgc ccctggaaaa tacgatgaat tctacaactt
     1021 tgtaagtggt ggatttagcg gtcagatggg agtttatgga cttccaagcg gacgtttgtt
     1081 gaaagttata ccaatattct ccgttaaccc tgaaaatggt tatggttaca gtgaagaaac
     1141 caagcctatg ttagaaacat cttatggttt tattccgtgg gatgatcagc accacctaga
     1201 actttctatg acaaacggtg tgcatgatgg aaaatggatt tttgccaatg caaataacac
     1261 cccacgtgtt gctcgtgttg acttaaaatc gtttaaaaca gtagagattt tagaaattcc
     1321 aaattctgct ggaaaccact cttctccgtt tgcaactgaa aacacagaat acatcgttgc
     1381 aggaacacgt ttctcagtac ctacagatga ttcaaatgga gacgttccaa ttgatagtta
     1441 caagcaaaac tttaagggcg tagtttcttt tatcagcgtt gataaagaaa ctggagacat
     1501 gaatttaagt ttccaagttg aagctccagg tgttaacttc gacttatctc gtgctggtaa
     1561 aggtgtttca aaagattggt tcttctttac ttgttataat tctgagcaag caaatacatt
     1621 gttagaagta aatgcttctc aatttgacaa agactttatc atggctttaa actggaaaaa
     1681 agcagaagca ttagttaaag ctggtaaagg aaaaaaaatc aaaacaaaat atgcacataa
     1741 taaattaagc gaagaaacgc atactgcaac ttctacaatg aaggaagatg tgacggtgtt
     1801 gactatcgat gatttgaaag atgcaattta catgattcct tgtccaaaat ctccacacgg
     1861 atgtgatgtt aaccctacag gagaatacat cattggttct ggaaaattag ctgcattaat
     1921 tcctgtattt agctttgaca aaattcaaaa aagtattagc tcaaaagact ttgcaggaaa
     1981 ttatggtggt attccaatta ttaaatatga atctgcattg tatggtgaag ttcaaaaacc
     2041 tggtttaggg cctttgcaca cagagtttga tgcgaatgga aatgcatata catccatgtt
     2101 cttatcttct gaagtggtta agtggaattt gaaatcattg aaaattgttg atagacaacc
     2161 aacattctac tctactggtc acttaatggt ttgtggtggt aacacagcta aaccttatgg
     2221 taaatacttg gttgcataca acaaaatcac aaaagaccgt tatttaccaa ctggtccaga
     2281 gttgacacaa tctgctcaat tatttgatat tagcggagaa aaaatggagt tattgttaga
     2341 cttcccaact atcggagaac ctcactatgc acaagcagct ccagctgatt tgattagcaa
     2401 aaatcaaatt aaaatgtata aaatagacca caacaaacat ccttatgtgt ctatgggtga
     2461 ggctaaaact aaagttgtaa gagaaggaaa taaagtacac gtttacatga catcaattcg
     2521 ttctcacttt gcaccggata atattgaagg aattagagtt ggagacgaag tttacttcca
     2581 cgttactaac ttagagcaag actgggattt accgcatggt ttcgcaatca aaggtaacca
     2641 aaatgcagag ttattaatta tgcctggtga aacaagtaca ttgaaatggg ttccaaacaa
     2701 agcgggaatg ttcccaatgt attgtacaga cttctgttct gcactacacc aagaaatgca
     2761 aggttatgtt cgtgtttctc cagcaggtag caacacaccg ttaatctaca gtcttaataa
     2821 caaaaaagac aatgcaagca gccctgttaa acaaggtgaa acaaaataaa aatcacaaaa
     2881 aggggcttga aaaatagccc ctttttatta atccttttat catgaatacc aataatttat
     2941 cccaaacaag caaattaacc ttttatctat taatcgtttg tgccattggt tttatcgtta
     3001 gtatcattac gccaatttgg tctatttacc tacttgctcc ccaatatcct gaaggtttaa
     3061 gtatgtattt acacgccaac aaattagcag gtgatgttga gattattaat ggtttaaatc
     3121 attatatagg aatgaaagaa ttgcacgcag acgatttctg ggaatttact tacctcccaa
     3181 caatccttgg tgtactaagt tttattacac ttcttgtagc attccttaaa aagaaaaaat
     3241 tcctttatgc gtttacaatt ttctatgcat tgtttggaat tgcttttatg gttgattttt
     3301 ggtggtggga ataccaatac ggtcacaatt tagatccatc agcagcaatt attatcccag
     3361 gaatgtctta tcaaccacct ttgtttggta aaaaagtttt actcaacttc actgcttt
//
LOCUS       DAOQVQ010000238         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31757_length_3411_cov_3.841478, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000238 DAOQVQ010000000
VERSION     DAOQVQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31757_length_3411_cov_3.841478"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKN22_07565"
     CDS             complement(<1..670)
                     /locus_tag="PKN22_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNS42600.1"
                     /translation="MKNDTPAVLILEDGTIFHGKAIGKIGTTTGEIAFNTGMTGYQEV
                     FTDPSYYGQILVMTTSHIGNYGVHDEEVESDAMMIAGLVTKKFSETFSRASASGSMEN
                     LMQQNEKVGISDVDTRALVRHIRSKGAMNAIISSDYTSIEDLKAQLAQVPSMSGLELS
                     SKVCTKEAYEVKPENPICKISVVDFGIKKNIVRCLVERGAHVKVFPMHTTLAELDTFQ
                     PDGYM"
     gene            complement(715..>3411)
                     /gene="carB"
                     /locus_tag="PKN22_07570"
     CDS             complement(715..>3411)
                     /gene="carB"
                     /locus_tag="PKN22_07570"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNS42601.1"
                     /translation="GIEVTLINSNPATIMTDKVVADNVYLQPLEPESIIEILKNHKID
                     AVLPTMGGQTGLNLAMKCEEMGIWEEYGVRMIGVDTTAIAITEDREQFRELMGKIGVP
                     MAPQKTAKSFLEGKEIAQEFGFPLCIRASYTLGGAGASIVYDSKEFDSLLERGLQLSP
                     IHEVMIDKAMMGWKEFELELLRDANDNVVIVCSIENFDPMGIHTGDSITVAPAMTLSD
                     TTFQKMRDMAIKMMRSIGNFAGGCNVQFAVSPDDKEEIIAIEINPRVSRSSALASKAT
                     GYPIAKIASKLAIGYRLDELKNQITKTTSAMFEPTLDYVIVKIPRWNFNKFPGTDRRL
                     GLQMKSVGEVMGIGRSFQEALQKACQSLEINRNGLGADGKELTDQREILHSLENASWN
                     RLFHIYDAIKLGIPFKTIQEKTKIDLWFLKQIEELIDLERKMEKYHLENIPKELLFEA
                     KQKGYADRQVAHLLRCLESEVHKKREEFGIKRVYKLVDTCAGEFEAQTPYYYSTFEYE
                     NESVVSDKKKIVVLGSGPNRIGQGIEFDYSCVHGVLAAKECGYETIMINCNPETVSTD
                     FDTADKLYFEPVFWEHIYDIIQHEKPVGVIVQLGGQTALKLAEKLNRYGIPIIGTSYE
                     ALDLAEDRGRFSKLLEENNILFPKYGVVESAEQALELSKDLGFPLLVRPSYVLGGQKM
                     KIVINEEELEHHVVDIIGEMGANQILLDHFIGGAIECEADAICDGEDVYIIGIMQHIE
                     PAGIHSGDSYAVLPPYNLGDLVMTQIEEITKKVAKALKTVGLINIQYAVKDDKVYVIE
                     ANPRASRTVPFIAKAYDEPYVNYATKVMLGEKKVKDFNFNPKKEGYAIKIPVFSYEKF
                     PDVKKELGPEMKSTGEGIRFIKDLRDPFFMKIYAERNMHLSK"
BASE COUNT          978 a          751 c          530 g         1152 t
ORIGIN      
        1 acatataacc atctggttga aaggtatcca gttctgccaa cgttgtatgc attggaaaca
       61 ctttaacgtg tgccccacgc tccaccaagc aacgaacaat gtttttctta attccaaaat
      121 caactacaga aattttgcaa attggatttt ctggttttac ttcgtaggct tcttttgtgc
      181 aaacttttga agacaattcc aacccactca ttgaaggaac ttgagccaat tgtgctttca
      241 aatcttcaat cgatgtgtag tcggatgaaa taatggcatt catggcacct ttactgcgaa
      301 tatgacgcac caatgctctt gtatccacat ccgaaattcc aactttttca ttttgctgca
      361 tcaagttttc cattgatcca cttgcagatg cgcgtgaaaa cgtttcagaa aactttttgg
      421 ttacaagtcc agcaatcatc attgcatcag actccacctc ttcatcgtgc acaccataat
      481 tcccaatatg tgaagttgtc attaccaaaa tttgcccata ataggaaggg tcggtaaata
      541 cttcttgata acctgtcata cctgtattga aagcaatctc accggttgta gtgccaattt
      601 tcccaatggc tttaccatga aatattgttc cgtcttcaag tattaaaact gctggtgtat
      661 cgtttttcat ttcttttatt ttattaggag taatttgctc aaattatcta ttaattactt
      721 cgataaatgc atgtttcttt ccgcataaat cttcataaag aatgggtctc ttaagtcttt
      781 tatgaatcga attccttctc cggttgattt catttctgga cctaattctt ttttcacatc
      841 tgggaatttt tcataagaga aaactggaat tttaattgca tacccttctt ttttaggatt
      901 gaaattaaaa tctttcactt ttttctctcc caacatcact tttgttgcgt aatttacata
      961 aggttcatcg tatgcttttg caataaatgg aacggttctc gatgcacgtg gattggcttc
     1021 aatcacatac actttatcgt cttttactgc atattgaata ttgattaaac ctactgtttt
     1081 taatgctttt gctacttttt tagtaatttc ttcaatttga gtcattacca aatcacctaa
     1141 attgtatgga ggcaataccg cataagagtc acccgagtga attccagctg gctcaatgtg
     1201 ctgcatgatt ccgatgatgt acacatcttc tccatcgcaa atagcatcgg cctcgcactc
     1261 aatggcacca cctataaaat gatccaacaa aatttggttg gcacccattt caccaataat
     1321 gtccacaaca tggtgctcca attcttcctc gttgataaca attttcattt tttgtccacc
     1381 caatacataa gaaggacgaa ccaataacgg gaatcctaag tcttttgaca attccaatgc
     1441 ttgttcagca ctttcaacca caccgtattt agggaacaat atgttgtttt cttccaataa
     1501 tttagaaaaa cgacctctgt cttcggctaa atccaatgct tcgtagcttg taccaataat
     1561 tggaataccg tagcgattta atttttcagc taatttcaat gctgtttgac cacccaattg
     1621 aacaattaca cccactggtt tttcgtgctg aataatgtca taaatatgct cccagaatac
     1681 tggttcaaag tacaatttat cggctgtatc aaagtcggtt gaaacggttt ccgggttaca
     1741 gttaatcatg atggtttcat aaccacattc tttagctgcc aacactccgt gaacacatga
     1801 gtaatcaaac tctattccct gtccaatacg gtttggtcca gaacctaaaa ctacaatttt
     1861 ctttttatcc gaaacaacgc tttcgttttc gtattcaaac gttgagtaat aataaggtgt
     1921 ttgtgcttca aactcaccgg cacacgtatc cactaattta tagacacgct tgataccaaa
     1981 ttcttcacgt tttttatgta cttccgattc caaacaacgc aatagatgcg ctacttgacg
     2041 atcggcataa cctttttgtt ttgcttcaaa caacaattct tttggaatgt tttccaaatg
     2101 atatttttcc attttacgtt ccaaatcaat caactcttcg atttgtttta agaaccacaa
     2161 gtcgattttt gttttttctt gaattgtttt gaacggaatt cctaatttaa tggcatcata
     2221 aatatgaaac aagcggttcc acgatgcatt ttcaagcgag tgcaatattt cacgttgatc
     2281 agtcaattct tttccatcgg cacccaaacc atttctgttg atttcaagtg attgacatgc
     2341 tttttgcaac gcttcttgga acgagcgtcc aattcccatc acctcaccta ctgatttcat
     2401 ttgcaatccc aaacgacggt cagttccagg gaatttatta aagttccaac gcggtatttt
     2461 aacaattaca taatccaacg ttggctcaaa cattgccgaa gttgtttttg taatttgatt
     2521 tttcaactca tccaaacggt atccgattgc caattttgaa gcaatttttg caattggata
     2581 accagttgct tttgatgcta aggccgatga acgcgataca cgtgggttga tttcaatagc
     2641 aataatttct tctttatcat ctgggctaac agcaaactgc acgttacaac cacctgcaaa
     2701 attcccgatt gaacgcatca ttttgatggc catatcacgc attttttgga aagttgtatc
     2761 cgacaatgtc atggcaggag ctactgtaat tgaatctcca gtgtgaattc ccattgggtc
     2821 aaaattctca atcgaacaaa caataacaac gttatcattg gcatcgcgca ataattccaa
     2881 ctcaaattct ttccatccca tcattgcttt gtcaatcatc acttcgtgga taggtgataa
     2941 ttgcaaccct ctttctagta aactatcaaa ttcttttgaa tcataaacaa tagaagctcc
     3001 agccccacct aaggtataag aagcacgaat acacaacggg aaaccaaatt cttgtgcaat
     3061 ttcttttcct tctaagaacg attttgctgt tttttgaggc gccattggaa ctccaatttt
     3121 acccatcaac tcacgaaatt gctcacgatc ttcggtaatg gcaatggcag tggtatccac
     3181 accaatcata cgcacaccat attcttccca aatacccatt tcctcgcatt tcatagcaag
     3241 gttcaaccct gtttgacctc ccatggttgg caacacagca tcaattttgt ggttttttag
     3301 aatttcaata atggattcag gttcaagagg ttgtaagtaa acattatccg ctacaacttt
     3361 atcagtcatg atagttgctg gattggaatt aattaatgta acttctattc c
//
LOCUS       DAOQVQ010000239         3406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31824_length_3406_cov_3.198448, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000239 DAOQVQ010000000
VERSION     DAOQVQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31824_length_3406_cov_3.198448"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKN22_07575"
     CDS             <1..600
                     /locus_tag="PKN22_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS42602.1"
                     /translation="DAIRMLLEKGIWKNVSESMPEAQLIVVGAYQNNTISQYCDMSIK
                     VKIAGQIATISEVYAPIKISLAPLRFGAGIKGKILEAMEQGVPFITTSIGAEGLFLDA
                     SWDACIADDWQEFEQKAITLYTDKEKWLHFQSKGWEHLQRYFSKQKHEQPFIDFVSNQ
                     LKNYAANRKKRYFSQLVQHHSLMSTRYLSKWIMEKNKNN"
     gene            complement(601..828)
                     /gene="xseB"
                     /locus_tag="PKN22_07580"
     CDS             complement(601..828)
                     /gene="xseB"
                     /locus_tag="PKN22_07580"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNS42603.1"
                     /translation="MKQKKSNFTPMTEEIKYTEAFEELQTIVADLEDGEISVDELSIK
                     IKRASELIKICKKKLVTTEEDVNQILKELEE"
     gene            complement(809..2200)
                     /gene="xseA"
                     /locus_tag="PKN22_07585"
     CDS             complement(809..2200)
                     /gene="xseA"
                     /locus_tag="PKN22_07585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNS42604.1"
                     /translation="MNEQRKIFTLKQVVSSIRKTIEERYQQFYWIKAEMHKLNLYPSG
                     HAFPELVYKEDGKIVAQINASIWKHNLQKINQQFVQILKEPLKEGSTLLLCAKISFSE
                     SYGISLQIIDIDPSYTLGELQRERQETLLRLQKEGLINKNQQVEFPLLPQRIAIISAD
                     TSKGLSDFMKVLNQNKFGYAFFTHLFFAYLQGDNAVDSITKQLQAIEKVKRHFDIVVI
                     VRGGGGEVGLACYNNYELCKAIANCSLPVLTGIGHSTNLTVAEMISYRNAITPTELGE
                     YLLQVFHEFSVPVDRALEKIQQLPLQLLAQNKLAFTQTVKHLKLNVQNNLLTHHNRWQ
                     TISRKLNTKTELSIQRQRERIGFLKQSLSSLTKRYCLEKQNYLNDASMFLRQNVQKKF
                     QQENDALILAQKHIQLLDPKNVLKRGYSIARYNGKTIQANNTPQTGEQLEIETFNYTI
                     SATTTVVNETKKE"
     gene            2362..2835
                     /locus_tag="PKN22_07590"
     CDS             2362..2835
                     /locus_tag="PKN22_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42605.1"
                     /translation="MKQKKTLTKTNDNLLEIRQETLKNVSNETIRPHGKLWGMDVFTW
                     NNPDVSMLASTIQAFPFPVVLLTPASLLMTVVDFDSTVKLNINATIVSIDDNCTLNMQ
                     LLNAIPTIALTESMEESLEELQQCKIPRGIVLFCQEGQDTQINTKKFLEFLTLHQ"
     gene            2839..>3406
                     /locus_tag="PKN22_07595"
     CDS             2839..>3406
                     /locus_tag="PKN22_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNS42606.1"
                     /translation="MDTTKEKAFVPSQFFSVNRLLNLLGGDKVIWIITLLLLAFSLLT
                     VFSFVPILVKVEGGTPFRYLFKHFIYIIISFVVMLWMHKRDPKYFSQVAKLSLYVGIG
                     LLFLTLLMPNEVNNAKRWIKVPFIGLTFQASDFAKVALIMFLSRQLAKKKDVFLDWKE
                     GFVPIASAILGVFILIFKDNFSTAAIIFA"
BASE COUNT         1029 a          565 c          642 g         1170 t
ORIGIN      
        1 gatgcaattc gtatgttgtt ggaaaaagga atttggaaaa atgtgagtga atccatgcca
       61 gaagcgcaat tgatagtggt tggagcgtat caaaataaca ccatatcgca atattgcgat
      121 atgtcgataa aggtgaaaat tgcaggtcaa attgcaacta tttcagaggt ctatgcaccc
      181 ataaaaatca gcttggcacc cttgcgtttt ggcgccggaa ttaaaggtaa aattttggaa
      241 gcaatggaac aaggagtgcc gtttattacc acttccattg gtgccgaagg tttgttttta
      301 gatgcgagtt gggatgcttg cattgctgat gattggcaag aatttgaaca aaaagcgatt
      361 acattatata ccgataaaga aaaatggttg cattttcaat ccaaaggatg ggagcacctg
      421 caacgttatt ttagcaagca aaaacacgag caacccttta ttgattttgt ttctaatcaa
      481 ctgaaaaatt atgcagccaa tcgcaaaaaa cgttatttct cgcaattggt tcagcaccat
      541 agtttaatga gcacgcgcta tttatccaaa tggattatgg aaaagaataa aaacaattaa
      601 ttattcttcc aattctttaa gtatttgatt gacgtcctct tcggtagtta ccaatttctt
      661 tttgcagatt ttaattagtt cggaggcacg ttttattttg atggaaagtt catcaacaga
      721 aatttcgcca tcttctaaat cggctacgat ggtttgtaat tcttcaaaag cttcggtata
      781 ttttatttct tctgtcattg gtgtaaaatt actctttttt tgtttcattc acaacggttg
      841 ttgttgcaga aatggtatag ttaaatgttt caatttctag ttgctcaccc gtttgaggtg
      901 tgttgttggc ttgaatagtt tttccattat aacgtgcaat gctgtatcct cttttcaata
      961 catttttagg gtcgaggagt tgaatgtgtt tttgtgccaa tattaaagca tcattttctt
     1021 gttgaaattt cttttgtaca ttttgacgta aaaacatgga cgcatcgttg aggtaattct
     1081 gtttttcaag gcaataacgt ttggttaacg aacttaaact ttgtttcaaa aacccaattc
     1141 gttcgcgctg acgttgaata gaaagctcgg ttttggtgtt taattttctg ctgatggttt
     1201 gccaacggtt gtgatgggtc aataaattgt tttgaacatt caatttaagg tgtttaaccg
     1261 tttgggtgaa ggctaattta ttttgcgcta acaattgtaa tggaagttgt tgaatttttt
     1321 ccaacgctct atccacggga acgctgaatt catgaaaaac ctgcaataaa tattcgccca
     1381 attctgttgg tgtaatggca ttgcgatacg aaatcatttc ggcaacagta aggttagtgg
     1441 agtgtccaat accagttaaa acaggcagcg agcaattggc aatggctttg cataactcat
     1501 aattgttgta gcaagcaagc ccaacttcgc caccgccacc acgaacaatc accacaatat
     1561 caaaatggcg tttaactttt tcaatggctt gcagttgttt tgttatggaa tcaaccgcat
     1621 tatcgccttg cagataagca aaaaagaggt gggtaaaaaa agcatagcca aatttatttt
     1681 ggttcaatac tttcataaaa tccgacaatc ctttactcgt atcggcagaa attatggcaa
     1741 tacgctgagg aagtaatggg aactctacct gctgattttt gttgatgagt ccttcttttt
     1801 gcaagcgcaa caaagtttct tggcgttcac gttgcaattc gcccagggta taacttggat
     1861 ctatgtcaat aatttgcaat gaaatgccgt aagactctga aaaactaatt tttgcacaca
     1921 ataaaagtgt ggaaccttct ttgagcggtt cttttaaaat ttgtacaaat tgctgattaa
     1981 ttttttgcaa gttgtgcttc caaatagatg cgttgatttg tgcaacaatt ttcccatctt
     2041 ctttatacac caattctgga aatgcatgac cacttggata taaattgagc ttgtgcattt
     2101 ctgctttgat ccaataaaat tgttgataac gttcttcaat tgtcttccga atagaggaaa
     2161 caacttgctt caacgtaaat atttttcttt gctcattcat actttgaaat tagtaaaaac
     2221 ttatggataa caaccaaatt attttcttga aaatccattt ttattttatt accaacaaac
     2281 tgttcataat ctgttttaaa cgacttcaaa ttgcatgttc ttatcattta tttttgttag
     2341 gttaaacatg aataaaagag catgaaacag aaaaagaccc taactaaaac taatgataat
     2401 ttactggaaa taagacaaga gacgttaaag aatgtgtcaa acgagaccat tcgtccacac
     2461 ggaaaattgt ggggaatgga tgtgtttacg tggaataatc cagatgtaag catgttggca
     2521 tccaccattc aagcatttcc atttccagtt gttttgttaa cacctgcatc cttgttgatg
     2581 acagttgtag attttgattc aactgtaaaa ttaaatatca acgcaacgat tgtttctata
     2641 gacgataatt gcacgttgaa catgcagctt ttaaatgcca ttccaacaat tgcactaact
     2701 gagtcaatgg aagaatcgtt ggaagaatta cagcaatgca aaattccacg tggcatagtt
     2761 ttgttctgcc aagaagggca agatacgcaa ataaatacaa agaaattttt ggagttttta
     2821 accctacatc aataacaaat ggatacaaca aaggaaaaag catttgttcc ttcccaattt
     2881 ttttcagtaa atcgtttgct aaatttactg ggaggcgata aagtaatttg gattattact
     2941 ttgttgttgc ttgctttttc attgttaacc gtttttagct ttgttccaat tttggtgaaa
     3001 gtggaaggtg gcactccatt tcgttatttg ttcaagcatt ttatttacat tatcatcagt
     3061 tttgtggtga tgttgtggat gcataaacgc gatccgaaat atttttcaca agttgctaaa
     3121 ctttcattgt atgttggaat tggcttgttg tttctcacac tcttaatgcc caatgaggtt
     3181 aataatgcaa aacgctggat aaaagtgcca tttattggtt taacctttca agcatccgac
     3241 tttgcaaagg tggcgcttat catgttttta tcgcgtcaat tagctaagaa aaaagatgtt
     3301 tttttagatt ggaaagaagg gtttgtgcct attgctagtg ctattcttgg tgtttttata
     3361 ttaattttta aagataactt ttcaacagca gccatcattt ttgcgt
//
LOCUS       DAOQVQ010000240         3400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31889_length_3400_cov_4.257100, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000240 DAOQVQ010000000
VERSION     DAOQVQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31889_length_3400_cov_4.257100"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKN22_07600"
     CDS             <1..495
                     /locus_tag="PKN22_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42607.1"
                     /translation="NPTDSTEIKNYNYQIANNNELHLVLPDFKGERVKITLQSGAIKA
                     SQGWTQQEYSAVFTKRFEKEFGTLNVNIQHYTQPIIFELLLAGKTVKKEMVAGDKKLQ
                     FTWLEPGEYSYRITVDANNNQRWDTGDFEQKIQPEEIHLYSTTTKVRANWEVDVELEP
                     IEKK"
     gene            complement(506..1657)
                     /locus_tag="PKN22_07605"
     CDS             complement(506..1657)
                     /locus_tag="PKN22_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS42608.1"
                     /translation="MKNEIEIKIIKLIESKREDDYWDFKREPHDNNASLLHDIICLSN
                     SLHKGNRYLIIGVSDPKDGAELIGLTKGQANRKSQVEYIDFIRTKPFSGHCRPEIELH
                     TIKIRDIEIDVLEIFDYPLKPYYLTEDYRDKDKNVRANFIYSRTNDTNTPIDKSTDVG
                     IIEKMWRQRFGLDLPPLERIKILLRQPQDWFKDIGNKPYAYHLDFPEFRIEFSETHEF
                     WEVFSYFFTNGKSYLGTATFKYHSTTLFELEYMYCDEMRIELAIPKIAIVRLKEYVNW
                     FYYYDLQELNGIFLQFITNKLTHLHSRNSAFPFVIFDNENERKEFIDYVVKNEDDLHN
                     IDPDNWSKQAKSEMEKQDKSSVIDPIFIDKITQLHKKWARHNSSFGNPS"
     gene            complement(1798..1995)
                     /locus_tag="PKN22_07610"
     CDS             complement(1798..1995)
                     /locus_tag="PKN22_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42609.1"
                     /translation="MSKTINHSNLKSLDQFVDEQYGKRGTKKREAFEQGFEAFKIGFL
                     TQQARLDKGIDTRRVCRKGGN"
     gene            complement(2001..>3400)
                     /locus_tag="PKN22_07615"
     CDS             complement(2001..>3400)
                     /locus_tag="PKN22_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNS42610.1"
                     /translation="IGGADFKRRYIGAYLSEDERFLFISAANTTNGNELYVKDLKNNS
                     DFIALQKGYDYNTDVIDTKGDFIYVVTDKNAPNKQLQKIDFNRPEKWYDVIPTNENVL
                     NVSTGGGYLFAHYMKDALSYIEQYDYEGKRIRVVELPGNGTVGGFGGKEKQQELYFTF
                     TNYITPGTIYKYDVNTGKSVIYQRPKVNFNPDDFVSEQVFFTSKDGTKIPMMISYKKG
                     IKLNGKNPTLLYSYGGFNISLQPSFSVVNAVWMENGGIYAVPNIRGGGEYGKAWHDAG
                     TKMQKKNVFEDFIAAGEYLKAKGYTSSKYLAISGRSNGGLLIGATMTMQPDFAKVAFP
                     GVGVLDMLRYNKFTAGAGWAYDYGTAEDNKEMFEYLHSYSPVHNVKKNVCYPSTMIIT
                     SDHDDRVVPAHSFKFGAELQEKQACNNPILVRIEVNAGHGAGRSTDQVIGENADLISF
                     ALKEMGVKKLKKAKK"
BASE COUNT         1035 a          657 c          532 g         1176 t
ORIGIN      
        1 aatccaacag attctacaga aattaaaaac tataattatc aaattgccaa caacaatgaa
       61 ttgcatttgg ttttacctga ttttaaggga gaacgtgtaa aaatcacctt gcaatcgggt
      121 gcaataaaag ccagccaagg gtggacgcaa caagaatatt ctgctgtgtt taccaaacgt
      181 tttgaaaaag aatttggcac tttaaacgta aacattcagc attataccca accgattatt
      241 tttgaattgt tgttagccgg aaaaacagtt aaaaaagaaa tggttgcagg cgataaaaaa
      301 ctccaattta cgtggttaga accaggagaa tatagttacc gcatcacagt agatgccaac
      361 aacaaccaac gttgggatac aggagatttt gaacaaaaaa ttcaacccga agaaattcat
      421 ttgtattcta caactaccaa agtgagagcc aactgggaag tggatgtgga attagagcct
      481 attgagaaga aatagtttta agtttttatg acggattacc aaacgagctg ttatgtcttg
      541 cccatttttt atgtaattgt gtgattttat cgataaaaat cgggtctata actgaacttt
      601 tatcttgctt ttccatttca ctttttgctt gttttgacca attatcagga tcgatattat
      661 gtaaatcatc ttcatttttt acaacataat caataaactc tttcctttca ttttcattgt
      721 caaaaataac aaaaggaaaa gcagaatttc ttgaatgtag atgagttaac ttgttggtta
      781 tgaattgaag aaaaattccg ttaagctctt gtaaatcata atagtaaaac caattcacat
      841 attcttttaa tctcacaatt gcaatctttg gaatagccaa ctcaattctc atctcatcgc
      901 aatacatgta ttctaattca aacaatgttg tagaatgata tttgaaagtc gcagttccta
      961 aataagattt tccgtttgta aaaaagtaac taaatacttc ccaaaattca tgtgtctcag
     1021 aaaattcaat tctaaattct ggaaaatcta agtgatatgc atatggttta ttacctatat
     1081 ctttaaacca atcttgcggt tggcgtagca agatttttat acgctccaaa ggtggtaaat
     1141 ccaacccgaa tctttgtctc cacatttttt caattattcc gacatccgtg gacttatcta
     1201 tcggagtatt tgtgtcatta gttcttgaat aaataaagtt tgctctgaca tttttgtctt
     1261 tatccctata atcttctgtt aagtagtatg gttttaatgg atagtcaaaa atttctaata
     1321 catctatttc aatgtctctt attttaattg tatgaagttc aatttcaggt ctacaatgac
     1381 cagaaaaagg ttttgttctt ataaagtcaa tatattcaac ttgtgacttc ctgttcgcct
     1441 gcccttttgt caacccaatc agctcagctc catcttttgg gtcagaaaca ccaataatta
     1501 ggtatctatt acctttatgt aaactattag ataagcaaat aatgtcgtgc aaaagtgatg
     1561 cattgttatc atgaggctct cgtttgaaat cccagtaatc atcttccctt ttggattcta
     1621 ttagttttat tatttttatt tcaatttcat ttttcatcta tgcggttcgt ttcttgggtt
     1681 agccataact ttattgatag ttccaaatga ccaccaaatc ctttttcaat tattttttga
     1741 agtgttgata aacgtatgtc tttaatattg ttttcaatac gtgagatata ccctttgtta
     1801 gttcccacct tttctgcaaa ctcttcttgt gtcaattcct ttatcaagac gtgcctgttg
     1861 ggttaaaaaa ccaattttaa acgcttcaaa accttgttca aacgcctctc tttttttagt
     1921 tcctctttta ccatattgtt catcaacaaa ttggtcaagc gattttaaat tagaatgatt
     1981 tattgtttta ctcatcactt ttattttttt gcttttttca attttttcac tcccatttct
     2041 ttcaatgcaa agctgattaa atcggcattt tctccaataa cttggtcggt tgagcggcca
     2101 gcaccatgtc cagcatttac ttcgatgcgc accaaaatag gattattgca cgcttgtttt
     2161 tcttgcaatt cagctccaaa cttaaacgag tgcgcaggta ctacacgatc atcgtggtca
     2221 ctggtaataa tcatggttga cggataacaa acattctttt tcacgttgtg cacaggagaa
     2281 taggaatgca aatattcaaa catttcttta ttgtcttcgg ctgtaccgta atcgtaggcc
     2341 caacctgcac ctgccgtaaa cttgttgtag cgcaacatat ccaatacacc cacaccagga
     2401 aaggcaactt ttgcaaaatc aggttgcatg gtcattgttg caccaatcaa taaaccaccg
     2461 ttagaacgac cagaaattgc taaatacttg cttgaagtat agccttttgc tttcaaatat
     2521 tcgcctgcag caataaaatc ttcaaaaaca ttctttttct gcatttttgt accggcatcg
     2581 tgccatgctt taccatattc accacctcca cggatgttgg gaacagcata aatgccacca
     2641 ttttccatcc aaaccgcatt aactaccgaa aatgaaggct gcaaactgat gttaaatcca
     2701 ccataagaat acaaaagtgt agggttttta ccgttgagtt ttatcccttt tttgtagctt
     2761 atcatcatag ggatttttgt tccatctttt gacgtaaaaa atacttgctc cgacacaaaa
     2821 tcatcggggt tgaaattcac ttttggtcgc tggtaaataa ccgatttccc cgtattcaca
     2881 tcgtatttat aaattgttcc aggtgtaatg taattggtaa acgtgaaata caattcttgt
     2941 tgtttttctt taccgccaaa tccgccaacc gtaccatttc cgggcaattc tacaacacga
     3001 atgcgcttgc cttcataatc gtattgttca atataagaaa gtgcatcttt catgtagtgg
     3061 gcaaataggt agccgccacc agtgcttaca ttcaacacgt tttcgttggt tggaattaca
     3121 tcataccatt tttctggacg attgaaatca attttttgca attgtttgtt gggcgcattt
     3181 ttatcggtta caacgtaaat aaaatcacct ttagtatcaa taacatcggt gttgtaatcg
     3241 tatccttttt gaagtgcaat aaaatccgaa ttgtttttca aatctttcac atacaactca
     3301 tttccgttag tcgtattggc tgccgaaata aacaaaaagc gttcgtcttc ggataaatag
     3361 gcaccaatgt aacggcgttt aaaatctgcc cctccaatca
//
LOCUS       DAOQVQ010000241         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31927_length_3398_cov_3.017350, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000241 DAOQVQ010000000
VERSION     DAOQVQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31927_length_3398_cov_3.017350"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKN22_07620"
     CDS             <1..451
                     /locus_tag="PKN22_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS42611.1"
                     /translation="NPFVCLNFQKEVEQKCWEAAGKTQKVPAQRMLDFVQGKKSTEFP
                     RSSYMPGIVSVDLTTVLPPFIAKRLQQAFKDFGVKMKGYLTNDAVLHAPESRTSSPIQ
                     IPRNPETLEHVEIAGLYPCAEGAGYAGGIISAAIDGMKCADAIASKM"
     gene            486..1925
                     /locus_tag="PKN22_07625"
     CDS             486..1925
                     /locus_tag="PKN22_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42612.1"
                     /translation="MKRIFQSALFIFAVSLLLIACGKKSTARPVAESVSSFLNGNTNI
                     ISFGAFNLNDVLNKTGYQDEPKIKILVNETFSQLQSSVNLETPVFYAVEGPLKDNNPA
                     ATYLFLEVKNSDSLKTNLVKNGFEVTDNKEFQYVSDGDMCLAFDKNIVTVIIKSGITD
                     EKALLTAVRKRLTGDVSTGYVADILNQKNDFTFGVNLANLYGTSNTDLADLSAEKQKE
                     LTDMMTNSFISGALNFNNGEMAFKVKNHFSDALKNKLFFLKDNNAKIVGNLGAGAPKA
                     GLSLNLDTKKLQEFLNEYAPNAIDDLTEEIGGPFALAMLAADGDISKLIDGRIGALVF
                     GDANNLMSGLTPDLNFFIGLAGQGKKFGETIKEQLADKFKKVELTNAGIAGSSSLDFV
                     GNSVQLPASAANFGKNSVDFFLDLSTVDAASFDLDGGMKFLELLKSVSLTYNSDGGEL
                     IIKTKDNKDNILKQVLQKALQSFEDQLAF"
     gene            1925..2830
                     /locus_tag="PKN22_07630"
     CDS             1925..2830
                     /locus_tag="PKN22_07630"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNS42613.1"
                     /translation="MNNDFITFNTKELEIPTLHRHLLGAVGPRPIALASTIDENGVAN
                     LAPFSFFNVFSANPPIVVFSPANSGRTADPKDTYFNVIKTPEVVINVVTYEMVQQVNV
                     ASCPYPAGTDEFVKSGFTPLKSETVQPFRVAESPVQLECNVLEVKELGTGGAAGNLVI
                     CEVTRIHISKNVMDETGMIDQHKIDLISRMGGPWYCRADKNSMFKLVRPLTVCGVGFD
                     NLPEDILKSTVLTANNLGQLAVIESIPDETAVNDYKLLELSDFFLSLEQNPEELELAL
                     HRHAKELLEEDKIEEAWMTLLAFNR"
     gene            2861..3256
                     /locus_tag="PKN22_07635"
     CDS             2861..3256
                     /locus_tag="PKN22_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNS42614.1"
                     /translation="MYKLTGTVKVISPTVQVSEKFAKREFVVTESSSMYPQDILFQLT
                     QDKCNLIDSVNVNDSIEVSFNLRGRVWTSPQGEVKYFNTLEAWRIERVGQPMGGGMPQ
                     GGPSEMNLNPTLPPIEKATAESEDDDLPF"
BASE COUNT         1150 a          567 c          672 g         1009 t
ORIGIN      
        1 caacccgttt gtgtgcttga attttcaaaa agaagtggag caaaaatgtt gggaagcagc
       61 cggaaaaaca caaaaagtac cagcacaacg catgttggat tttgtgcaag gaaaaaaatc
      121 aaccgaattt ccacgaagtt cctacatgcc aggcattgtt tcggttgatt taacgacggt
      181 tttacctccg tttattgcca aacgtttgca acaagcattc aaagattttg gcgtaaaaat
      241 gaaaggttat ttaaccaacg atgctgtttt gcatgcgccc gaatcgcgca cttcatcgcc
      301 tatacaaatt ccacgcaacc ctgaaacatt ggaacacgtt gaaattgctg gactttatcc
      361 atgtgctgaa ggagcaggat atgcaggagg aatcatctct gctgcaattg acggcatgaa
      421 atgtgcagat gccattgcat cgaagatgta attttattaa ttttgaaacc gaaaatcaat
      481 ttattatgaa aagaatattt caatcagcgt tatttatttt tgctgtatca ctactattaa
      541 tcgcttgtgg aaaaaaatca accgctcgtc ctgttgccga aagtgtttcg tctttcttaa
      601 atggcaacac aaatatcatc agttttggag catttaactt aaacgatgtt ttaaacaaaa
      661 caggatacca agatgaacca aaaattaaga ttttagttaa cgagactttt agtcagttgc
      721 aatcaagtgt gaatttagaa acacctgttt tttatgctgt tgaaggtcct ttgaaagata
      781 acaatccagc tgcaacctat ttgtttttag aagtaaaaaa cagtgattca ctaaaaacta
      841 acttggttaa aaacggtttt gaagtaactg ataacaaaga atttcaatac gtttctgatg
      901 gcgacatgtg cttggcattt gataaaaaca ttgtaacggt tatcattaaa tcggggatta
      961 ctgacgaaaa agcattgtta acagctgtca gaaaacgttt aactggagat gttagcacgg
     1021 gttatgttgc cgatattttg aaccaaaaaa acgatttcac ttttggtgtt aatttggcta
     1081 atctttatgg cacctccaac accgatttgg ctgatttaag tgctgaaaaa caaaaagaat
     1141 taaccgatat gatgaccaac tcattcattt ctggagcact gaatttcaac aatggagaaa
     1201 tggcttttaa agtaaaaaat catttttcgg atgcgttgaa aaacaaatta tttttcttga
     1261 aagacaacaa tgcaaaaatt gttggtaatc taggtgcagg ggctccaaaa gctggtttat
     1321 cactaaatct tgacactaaa aaattacaag agttcttgaa tgaatatgct ccaaatgcaa
     1381 ttgatgactt gaccgaagaa attggtggtc cgtttgctct tgcaatgctt gctgcagatg
     1441 gcgatatttc taaattaatt gacggtagaa ttggtgcctt ggtgtttggt gatgccaaca
     1501 atttgatgag tggcttaaca cccgacctta atttctttat aggattggca ggtcaaggca
     1561 aaaaatttgg agaaacaatt aaagaacagt tagccgataa attcaaaaaa gttgagttaa
     1621 ccaatgctgg aattgcaggt tctagctcac ttgattttgt tggaaacagt gttcaattgc
     1681 cagcaagtgc agctaatttt ggaaaaaact cggttgattt cttcttagac ttaagcactg
     1741 ttgatgctgc ttcttttgat ttggatggag gaatgaaatt tttagaatta ttaaaaagtg
     1801 tttcgctcac ctacaacagt gatggtggtg aattaatcat taaaacaaaa gacaataaag
     1861 acaatatttt aaaacaagta cttcaaaaag cattgcaatc ttttgaagat caattagcat
     1921 tttaatgaac aacgatttta taacatttaa caccaaggaa ttagaaattc ctacattgca
     1981 ccgtcatttg ttgggagctg tgggtccacg cccaattgca ttagcctcta ccattgatga
     2041 aaacggagtt gccaatttag cgccttttag ttttttcaat gtgtttagtg ccaatccacc
     2101 aattgtagtg ttttcgcctg caaacagtgg aagaacagcc gatccaaaag atacatattt
     2161 caacgtcata aaaacgccag aagtggtgat taacgttgtt acctatgaaa tggttcaaca
     2221 agtaaacgtt gcaagttgtc cctatcctgc tggaacagat gaatttgtga aatcggggtt
     2281 cacacctttg aaatcagaaa cagttcaacc atttagagtg gccgaatcgc cagttcaatt
     2341 agagtgtaat gtattagaag tgaaagaatt gggaacagga ggagcagcgg gtaatttggt
     2401 aatttgcgaa gttacacgca ttcatatctc caaaaatgtg atggatgaaa caggcatgat
     2461 agaccaacat aaaattgatt taatttctcg catgggtggt ccgtggtatt gcagagccga
     2521 caaaaattcg atgtttaaat tggttcgtcc gttaaccgtt tgtggtgttg gttttgacaa
     2581 tcttccagag gacattctca aaagcaccgt gttgacagca aacaatttag gccaattagc
     2641 agtaattgaa agcattccag acgaaactgc tgtaaatgat tacaaattat tggaattaag
     2701 tgattttttc ctatctttag aacaaaatcc ggaagaatta gagttggcat tacatcgtca
     2761 tgcaaaggaa ttacttgaag aagacaagat tgaagaagca tggatgacct tactagcatt
     2821 caacagataa taacgaacaa caataaaatt aataattagt atgtataaat taacaggaac
     2881 agtaaaagtt atcagcccta ctgtacaagt ttcagaaaaa tttgcaaaaa gagaattcgt
     2941 agttacggaa tcatcttcaa tgtatccaca agacattcta tttcaattaa cacaagacaa
     3001 gtgtaattta attgattcgg ttaatgtaaa cgattcaata gaagtttcgt tcaacttacg
     3061 tggaagagtg tggacaagtc cgcaaggaga agtaaaatat ttcaacacat tagaggcttg
     3121 gcgcattgag cgtgttggtc agccaatggg tggaggaatg ccgcaaggtg gaccatcaga
     3181 aatgaattta aaccctactc tacctccaat tgaaaaagca acagcagaaa gcgaggacga
     3241 cgatttaccg ttctaattag ctaaaaatat taaaaaatgc tgtaaggtgt cgaggttaac
     3301 ttggcacctt ttttaatttt tatgttccaa gacgtgaatt tgcaagtttt ttgcttcaaa
     3361 atttacccga ttggggttcc aaacaaagaa tttcaatt
//
LOCUS       DAOQVQ010000242         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31943_length_3397_cov_3.829144, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000242 DAOQVQ010000000
VERSION     DAOQVQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31943_length_3397_cov_3.829144"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..480)
                     /locus_tag="PKN22_07640"
     CDS             complement(76..480)
                     /locus_tag="PKN22_07640"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNS42615.1"
                     /translation="MKTSRVEYMGGLRTKCTHLRSGSEIITDAPLDNNGKGEAFSPTD
                     LVATAYASCMMTIIGIFCNEHGLNFTHAVAEVTKIMGTAPRRISEIVIELDMTENGWN
                     EEEKVRVLRAGKACPVAKSVHENIVITFDIKL"
     gene            596..856
                     /locus_tag="PKN22_07645"
     CDS             596..856
                     /locus_tag="PKN22_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42616.1"
                     /translation="MSYYKTIDGKKYDGKLIELADELIAGSGDGRISMADAEKLLAAV
                     KDGDSYTDIEKETMAYLRENYKWTEAADEWFRTEIRKWAATK"
     gene            1026..1880
                     /locus_tag="PKN22_07650"
     CDS             1026..1880
                     /locus_tag="PKN22_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNS42617.1"
                     /translation="MQAFVGEISAYAKALKPGFVVIPQNGIQLAFNNAKSENGINLNY
                     LEAIDGFALEELFYNKKALDVDERYDYLQELKQYKKIMVSEFVKNSDDIADAYQKNNN
                     ENFACFVRSNDNYYYNQIPSSVYNENSNNIAQLAEIKNFLYLINAENYSSKQQLIDAL
                     RNTNFDLLLIDLFFNDEQISAADIAQLKTKKNGAQRLVISYMNIGSAENYRYYWKSDW
                     KLHHPKWIKKRYKGYKDEYYVEFWNQEWKDVIYGNNESYLKKIIDTGFDGVFLDNVEA
                     YYFLYKNN"
     gene            1977..3260
                     /gene="eno"
                     /locus_tag="PKN22_07655"
     CDS             1977..3260
                     /gene="eno"
                     /locus_tag="PKN22_07655"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNS42618.1"
                     /translation="MTYIVKIVGRQILDSRGNPTVEVDVVTSNGIVGRAAVPSGASTG
                     IHEAVELRDGDKAVYMGKGVLKAVANVNGPINEALEGMDVTEQKIIDKVLLELDGTDN
                     KSKLGANAILGTSLAVARAAAQESGLPLYQYVGGVGAVTLPVPMMNILNGGSHADNKI
                     DIQEFMVMPFGASSFSEGLRWGTEIFHNLKNVLKEKGMSTNVGDEGGFAPNLGSNEEA
                     IQVVLEAITRAGYKPGEDVWIALDAASSEFYKDGKYHFESTGESRTSEEMVAFWKEWV
                     EKYPIISIEDGLAEDDWAGWKLLTETIGSKVQLVGDDLFVTNTKRLKQGIEANTANSI
                     LVKVNQIGTLTETIEAVQLATASSYTSVMSHRSGETEDNTIADLAVALNCGQIKTGSA
                     SRSDRMSKYNQLLRIEAELGDAAYYPGKNFKFIKK"
BASE COUNT         1197 a          565 c          616 g         1019 t
ORIGIN      
        1 cagcacgtac tccaaaaata atgtctgtat cttctttttt ctctttgcgt tgaaattttc
       61 gttgttcctc tctcattaca atttaatatc aaaggtaata acaatgtttt cgtgcaccga
      121 tttagccacc ggacaagctt ttccagctct taacacacgt actttttctt cttcattcca
      181 accgttttcg gtcatatcca actcaatcac aatttcagaa atgcgacgtg gagctgttcc
      241 cattattttt gtaacctcag ccactgcatg tgtaaaattc aaaccatgct cgttgcaaaa
      301 aattccaatg atggtcatca tacacgaagc ataagccgtt gcaactaaat cagtaggtga
      361 aaatgcttct ccttttccat tattatccaa cggtgcgtct gtaataattt cagaacctga
      421 acgtaaatgc gtacactttg ttctaagtcc acccatgtat tcaactcgtg atgttttcat
      481 gttaattttg aatatttaga caaaagtact caaataaaaa ccattttttt agaaacactt
      541 gcattaattt acaattatcc ttacatttac taaaacattt taaaaaccct attttatgag
      601 ttattacaaa acaattgacg gaaaaaagta cgatggaaaa ttaattgaac ttgcagatga
      661 attaattgct ggttctggtg atggaagaat ttctatggct gatgctgaaa aattattggc
      721 agctgtaaaa gatggtgatt catataccga cattgaaaaa gaaacgatgg cttatttgcg
      781 tgaaaattac aaatggaccg aagcagctga cgaatggttt agaactgaaa tcagaaaatg
      841 ggctgcaaca aaataaatgc tccatttctt tcgttattaa aaggtgttat attagcacct
      901 tttttttgtt ctacttttaa attacaaaca tgaaaattat ctcttccata tttaaccttt
      961 taataatcag tatctttctc ttcagttgta ataaagaaga aaaaatcgaa aaagcagctt
     1021 tagaaatgca agcttttgta ggtgaaattt cagcttatgc aaaagctctt aagccaggat
     1081 ttgttgtcat tccccaaaac ggcattcaat tagctttcaa caacgcaaaa tctgaaaacg
     1141 gaatcaacct taattacctt gaagcaattg atggctttgc attggaagaa ttgttctaca
     1201 acaaaaaagc acttgatgtg gacgaacgtt acgattattt gcaagaactc aaacaataca
     1261 aaaaaataat ggtgtctgaa tttgtaaaaa acagcgatga cattgccgat gcttatcaaa
     1321 aaaacaacaa cgaaaatttt gcctgttttg taagaagcaa cgataattat tattacaacc
     1381 aaatcccatc ttcggtttat aatgaaaata gcaacaacat tgcccaacta gctgaaatta
     1441 aaaacttttt atacctcatc aatgcagaaa actacagttc gaaacaacaa ttaattgatg
     1501 ctttgcgtaa caccaatttt gatttactgc tcatcgattt atttttcaat gatgaacaaa
     1561 taagcgctgc tgacattgca caactcaaaa caaagaaaaa tggtgcacaa cgattggtta
     1621 tttcctacat gaacattgga tcggcagaaa actaccgtta ttattggaaa agcgattgga
     1681 agttgcatca ccccaagtgg attaaaaaac gctacaaagg ttacaaggac gagtactatg
     1741 tagaattctg gaatcaagaa tggaaagatg taatttatgg caacaacgaa tcctatttaa
     1801 agaaaatcat tgacactggg tttgatggcg ttttcttaga caatgttgag gcttattatt
     1861 ttttatacaa aaacaattaa gtaattaaca ataaatatgt ttttgtaaat atctttctcg
     1921 ttgattcgtc aaaaaaatta aaacgaaatc ataactttgc aaaaaaaatt aaaagcatga
     1981 cttatatcgt taaaattgtt ggaagacaaa ttcttgattc aagaggaaat cctactgtag
     2041 aagtagatgt tgttacgtca aacggaattg ttggtagagc tgccgttcca tcgggtgcat
     2101 ctacaggtat tcacgaagca gttgaattgc gtgatggtga caaagctgtt tacatgggaa
     2161 aaggtgtttt aaaagcggtt gcaaacgtaa atggaccaat caatgaagca ttggaaggaa
     2221 tggatgtaac ggagcaaaaa atcatcgata aagtactttt ggaattagac ggtacagata
     2281 ataagtcaaa attaggtgcc aatgccattt taggaacatc gttggcggtt gcacgtgctg
     2341 ctgcacaaga atctggatta cctttgtatc aatacgttgg tggtgttgga gcggttactt
     2401 taccagttcc aatgatgaac attttgaatg gaggttcaca tgccgacaat aaaattgaca
     2461 ttcaagaatt catggtaatg ccttttggtg cttctagttt ttctgaagga ttgcgttggg
     2521 gaacagagat ttttcacaac ctaaaaaatg tattaaaaga aaaggggatg tcaaccaatg
     2581 taggtgacga aggtggattt gctcctaact tgggttctaa cgaggaggca attcaagtag
     2641 tattagaagc aattacaaga gctggataca aaccaggaga agacgtttgg attgcattag
     2701 atgctgcatc ttctgaattt tacaaagacg gaaaatacca ttttgaatcg actggagaat
     2761 caagaacttc tgaagaaatg gtcgcatttt ggaaagaatg ggttgaaaaa tatccaatta
     2821 tttctatcga agacggattg gcagaagacg attgggctgg atggaaatta ttgacagaaa
     2881 ctattggttc gaaagtacaa ttagtaggcg acgatttatt tgttaccaac accaaacgtt
     2941 tgaaacaagg tattgaagca aacacagcca attccatctt ggtaaaagta aatcaaattg
     3001 gtacgttaac cgaaactatt gaagcagtac aattagccac tgcaagttca tacacttctg
     3061 taatgagcca ccgttctgga gaaacagaag acaataccat tgctgattta gcagtagcat
     3121 tgaactgtgg tcaaatcaaa actggttctg catctcgctc tgatcgtatg tcaaaataca
     3181 accaattatt gagaattgaa gcagaattgg gtgatgcggc ttattaccca ggaaaaaact
     3241 ttaagtttat taaaaagtaa ttacaaaaca tataaaatgg agttcaaatt gttgggctcc
     3301 atttttttgt aacgatttag ttaaaaaagc gttgctttta aaagcaacta atactaaaaa
     3361 ctaacgtcat tgaaaagaca caacagcaat aaaatga
//
LOCUS       DAOQVQ010000243         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_31997_length_3393_cov_3.496105, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000243 DAOQVQ010000000
VERSION     DAOQVQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31997_length_3393_cov_3.496105"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..944)
                     /locus_tag="PKN22_07660"
     CDS             complement(33..944)
                     /locus_tag="PKN22_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42619.1"
                     /translation="MKRVAVIDLGTNLFNLLIAAVEPTKFDVLFHTKSGVAIGMGGIN
                     NGIITNDAIQRAIQTLKEFKQHCDTYQVDEIKAFGTSAIRDAKNNQEFCAFIHQETGI
                     EVQIIDGLQEAQLIYQGVKWTYDFSEPTVVVDIGGGSTEFIVANQTGMQTAFSLNIGI
                     SRIFQQLTLSDPLTPEDIATIENWLEKHSEGKLKSIACSTVVGASGTFETLYQIQYQQ
                     EFPKSFECIEMTMDNISEILEEIIHSTLHERQLNTLIPPIRQIMMAIAGVKIRWIFKQ
                     LNAQKFFISPNSLKEGALYSASNLKKT"
     gene            complement(949..2286)
                     /locus_tag="PKN22_07665"
     CDS             complement(949..2286)
                     /locus_tag="PKN22_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42620.1"
                     /translation="MKNTWIVVSREIKERIASRSFVLFSVFGPLVILGLIYLLFKFGG
                     NEAEKWNVLVTDKAGLLENKMLAHPDKNVNYSFANDYIEHTNFADGKRYQQFDAMVEI
                     NEKVLSNKTAYVFYRTKPSFNLQAKVQYQVERRLEELMIKQFTQLSVAKFREIKQPLK
                     FNFRNVYDPYDQTSDIRTWVGFAFGVIILVFIALFGMTILRSVTSEKSNRIVEVLLAT
                     IQPKQLLFGKMAGIGIAAFIQFIIWIAIIGGGLFLMRAFLFPSIYDPSAMDIPALSDG
                     ANLLSAQDKLYNGGTFNAVVDLVYERVNFPVMLTYFFLFFVGGYLFYGSFFAAIGSTA
                     GTESDGQQFVLPILLILSLSAYAGYFAMSNPESELSSWLHYIPFTSPVVVMVKLAYGY
                     GAGEAYQLWLSFIVLLLSAFACLSIAARLYKNGVLQFGHRLRIKHLFQWLKMK"
     gene            complement(2290..3207)
                     /locus_tag="PKN22_07670"
     CDS             complement(2290..3207)
                     /locus_tag="PKN22_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS42621.1"
                     /translation="MLSVENISKSFLKKQALDAVSFKVNKGEVFGLLGPNGAGKTTLI
                     RIINQIIEPDSGVVRFNNDIATRRHLENIGYMPEERGLYKSMTVEDHALFLGRLRGLT
                     KNDARKKLNYWLEKFEITDWKNKRIEELSKGMAQKVQFICTVLHEPDLLILDEPYSGF
                     DPVNMELIKNELITMRNNGKTIILSTHNMKSVEEVCDSVALIHHSKKLLDGKVSNIQA
                     ERKTGVYAIKFRGNMIAFANALWAGYEVIDTSNPSDNLFVVKVKMRNDNNFDDLLRTL
                     IGQVQLEAAWEVMPSMQDIFIDIVQPKIA"
BASE COUNT         1078 a          661 c          599 g         1055 t
ORIGIN      
        1 caattgaata aggcgttaaa tttaagaatt atttaagttt ttttaaggtt tgaagcagaa
       61 taaagtgcac cttctttcaa cgagttgggt gaaatgaaga atttttgtgc attcaattgt
      121 ttgaatatcc aacgaatttt aactcccgca atggccatca taatttgacg aataggaggg
      181 atgagcgtgt tcaattgccg ctcatgcaag gtggaatgaa tgatttcttc taaaatttct
      241 gatatgttat ccattgtcat ttcaatgcac tcaaaagatt ttggaaattc ttgctgatac
      301 tggatttgat acaaggtttc aaacgttcca gaagctccaa ccaccgttga acaagcaatg
      361 ctttttaatt ttccttcgga atgtttttcc aaccaatttt ctatggttgc aatatcctct
      421 ggtgtcaatg gatcgctgag tgttaactgc tgaaaaatgc gtgaaattcc aatgtttaaa
      481 ctaaaagctg tttgcatacc cgtttggttg gcaacaataa attcggtact tcctccgcca
      541 atgtcaacaa caacggtggg ttcgctaaaa tcataggtcc atttcacacc ttgataaatg
      601 agttgcgctt cttgcaaacc atcaattatt tgcacttcaa ttccagtttc ttggtgaatg
      661 aatgcacaaa attcttggtt gttttttgca tcgcgaatgg ccgaagtccc aaatgctttg
      721 atttcatcta cctgataagt atcgcaatgt tgtttgaatt cttttaaggt ttgaatagct
      781 cgttgaatag catcgttggt gataattcca ttgttgatgc ctcccatgcc aatagcaaca
      841 cccgatttgg tgtgaaataa tacgtcgaat tttgtaggtt caactgctgc aatgagcaaa
      901 ttgaataaat tggttcctaa gtcaataacg gcaactcgtt tcatcaaatt atttcatttt
      961 caaccattga aacaagtgct taatgcgcaa gcggtgacca aattgcaata caccattttt
     1021 gtataaacgg gctgcaatag acaaacaagc aaatgccgaa agcaacaata caataaacga
     1081 caaccacaat tgatacgctt cgccagcacc ataaccatag gctaatttca ccattaccac
     1141 cacaggagaa gtgaatggaa tatagtgcaa ccaactgctc agttcacttt ctggatttga
     1201 cattgcaaag taacctgcgt atgctgataa ggataaaatc aaaagtatag gcaaaacaaa
     1261 ttgttgacca tcactttctg ttccggctgt tgagccaatt gctgcgaaaa atgaaccata
     1321 aaacaaataa cctccaacaa aaaataaaaa gaagtaggtg agcattactg gaaaattgac
     1381 gcgctcatat accaagtcaa caactgcatt aaaagtgcct ccgttgtata atttatcttg
     1441 tgccgaaagt aaattggctc catccgaaag agctggaata tccattgctg atgggtcgta
     1501 aatgctagga aataaaaatg cacgcattaa aaacaaacct ccaccaatga tggcaatcca
     1561 aatgatgaat tgaataaaag ccgcaattcc aatcccggcc attttgccaa acaacaactg
     1621 cttgggttga atagttgcca ataaaacttc tacaatgcga ttgctttttt cagaggtaac
     1681 gctgcgcaaa atagtcatac caaataaagc aataaacacc agaatgatga cgccaaatgc
     1741 aaatccaacc cacgtgcgga tgtcggaagt ttgatcgtag gggtcgtaca catttctaaa
     1801 attaaacttt aatggttgtt tgatttcgcg gaatttggca acagaaagtt gtgtaaattg
     1861 tttgatcatc aactcttcca aacggcgctc aacttgatat tgcaccttgg cttgcaagtt
     1921 aaacgaaggt ttggttctgt aaaaaacata agctgttttg ttgctcaata ctttttcgtt
     1981 gatttcaacc atggcatcaa actgttggta tcgtttaccg tcagcaaaat tggtgtgttc
     2041 aatataatcg ttggcaaaag aataattgac gtttttatct ggatgagcca acattttgtt
     2101 ttccaacaaa cctgctttat cagtaaccaa aacattccat ttttcggctt cattacctcc
     2161 aaatttaaac aacaggtaaa tcaaacctag aataacaagt ggaccaaaaa ctgaaaaaag
     2221 tacaaacgaa cgcgaggcaa ttctttcttt aatttcgcgc gatacaacta tccaagtatt
     2281 tttcattgat taggcaattt taggttgaac aatatcgata aaaatatcct gcatcgatgg
     2341 catcacttcc catgcagctt ctagttgcac ttgaccgata agagtgcgca ataaatcatc
     2401 aaaattattg tcgttgcgca tttttacttt caccacaaac aaattatccg aaggattgga
     2461 agtgtctatt acttcatagc ctgcccacaa agcgttggca aaagcaatca tatttcctct
     2521 aaatttaatg gcataaactc cagttttgcg ttcagcttgt atgttgctca cttttccgtc
     2581 caacaatttt ttagaatgat gaatcaatgc aacactgtca cacacttctt ccacactttt
     2641 catgttgtgg gtagaaagaa tgattgtttt tccgttattg cgcatagtaa tcaattcatt
     2701 tttaatcaat tccatgttaa cagggtcaaa accactgtat ggctcgtcta aaatcaacaa
     2761 atctggttcg tgcaatacgg tgcaaataaa ctgaactttt tgcgccattc ctttggaaag
     2821 ctcttcaata cgtttgtttt tccagtcggt aatttcaaat ttttccaacc aataatttaa
     2881 ctttttgcgg gcgtcatttt tggttaaacc tcttaatctg cctaaaaaaa gtgcatgatc
     2941 ttcaaccgtc attgatttat acaaaccacg ttcttcgggc atatagccga tgttttcaag
     3001 gtgccgacga gttgcaatat cgttgttaaa acgcaccacg ccagaatcgg gttctataat
     3061 ttgattaata atgcgaataa gcgttgtttt tccggctcca tttggaccca atagtccaaa
     3121 aacttcacct ttattcactt taaacgaaac ggcatcaagg gcttgtttct ttaaaaagct
     3181 tttggatata ttttctactg atagcataac tatttttgca gggttggatt tcaaagataa
     3241 aaataatcgg ttgtctttgg tggtaggagc aaaaaaatag gtgaatgaaa ttacaaatct
     3301 gcatattcca tggcatacaa atcaattttt tcgtctaaat aaatttcaaa atcggctttt
     3361 tcttcgggag caaaataggt tggaatattg agc
//
LOCUS       DAOQVQ010000244         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32102_length_3384_cov_3.965755, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000244 DAOQVQ010000000
VERSION     DAOQVQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32102_length_3384_cov_3.965755"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKN22_07675"
     CDS             complement(<1..1163)
                     /locus_tag="PKN22_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNS42622.1"
                     /translation="MSQEINNSNEIICPNCHKAFKVDQAGYASILKQVRDHQFEEEVK
                     KRLDLAQKEKENAVQLAQATIKNELQELIAKKDRELAELRAQKELELTQKITQKENEL
                     VQLQSKIEAAELNKKLAVTEATQKVEKERDELKSQLNIKETEKALLEKSLNEQFASKL
                     QEKEAIIRMKDEEIEMRKDLKMKMSTKMLGETLEQHCELEFNKLRATAFQKAYFEKDN
                     DARTGSKGDFIYREMDDAGNEIISIMFEMKNEGDETASKKKNEDFFKELDKDRTEKKC
                     EYAVLVTMLEAENEYYNTGIVDVSHRYEKMYVVRPNFFIPIITLLRNAAMNSLQYKAE
                     LALVRNQNIDISNFEDKINAFKEGFARNYELASRKFKTAIEEIDKTILHLQKT"
     gene            complement(1298..2473)
                     /locus_tag="PKN22_07680"
     CDS             complement(1298..2473)
                     /locus_tag="PKN22_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42623.1"
                     /translation="MKKRVLILSLFIATSGIFAYAENNVQTTTKQEISKAGVTFTVNV
                     KSDKGPVVGALVKVTLGNTVLGAGTTDAAGNAKITIASYNKQLVRIDVSHTLYRNDSK
                     SEVVLENDGIIPFVLKSKTESIDDAAAREAEYKAKQEAANKAAEEAKKQEDAIKQSTQ
                     QTQSEAEATKAAAEKKAAEAKAQQEALDKQRKEMEERLAAEKAAREEAAKKQQEQAQK
                     NAENKVVDASKEAKLTAEDAKREQKEQEAKLKEAQAQAKAQKEAAASEAERLKAAEKA
                     KKEAAKEQKAAEKAKKEAEKAAKEAEKEAKAAEKAKAQAEKLQANIEKLDEQQAKLNA
                     NRDKLNKQQQKLQSQLDKGKGDAAKIQTKMAKLDLSHAKLDESQLKIDKKREKLLKK"
     gene            2569..>3384
                     /locus_tag="PKN22_07685"
     CDS             2569..>3384
                     /locus_tag="PKN22_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNS42624.1"
                     /translation="MQFSEVIGQHHLKQLLINEINSKKISHAKLFLGKEGHGVLPLAL
                     AYVQYLFCKNKQENDSCGTCDSCLKVQNLQHPDLHFIYPIALAEKKSCADLYPKWRSK
                     IKENPYFSLEDWTNFIDPKGRRLIIGVDASTEILKSLTLKAFEGRQRVVVIWMAEEMN
                     DSAANKILKVLEEPPLGTYFILVGRQHDKFLTTILSRTQFYRVPKIETTDLSIHLREA
                     FELNISKADSITLRSEGDYLEAVQMLHDSSDINEERELFIELMRSCYKKDVNKM"
BASE COUNT          851 a          629 c          535 g         1369 t
ORIGIN      
        1 tttgtttttt gcaagtgtaa aatcgttttg tcaatttctt caatggctgt tttaaatttt
       61 ctacttgcta attcataatt tcgagcaaat ccttctttaa acgcattgat tttatcttca
      121 aaattagaaa tgtcaatatt ttgatttctc accaaagcta attcggcttt gtattgcagt
      181 gaattcatag ctgcgttgcg caataaggta ataattggaa tgaagaaatt tgggcgcacc
      241 acatacattt tctcataccg gtgagacaca tctacaattc ctgtgttata atattcgttt
      301 tcagcttcca acatggttac caaaacagca tattcacatt ttttttctgt tcgatctttg
      361 tccaattctt tgaaaaaatc ttcgtttttc tttttagagg cagtttcatc gccttcgttt
      421 ttcatttcaa acataatgga aatgatttca ttgccagcgt cgtccatttc acgataaata
      481 aaatcgcctt tacttcctgt gcgggcgtcg ttatcttttt caaaataagc tttttgaaat
      541 gctgttgcgc gcaatttatt gaactccaat tcgcaatgtt gctccaaagt ttcgcccaac
      601 attttggttg acattttcat ctttaggtct ttgcgcatct caatttcttc atctttcatt
      661 cgaatgatgg cttctttttc ttgtagtttt gaggcaaatt gttcgttgag cgatttctcc
      721 aataatgctt tttcagtttc ctttatgttt aattgacttt tcaattcatc gcgttctttt
      781 tcaacttttt gagtggcttc tgttactgcc aattttttat tcaattcagc agcctcaatt
      841 ttggattgca attgaaccaa ttcattttct ttttgggtga ttttttgggt taattccaat
      901 tctttttgtg ctctcaactc ggccaactct ctatcttttt ttgcaattag ttcttgcaat
      961 tcatttttta ttgtagcttg agcaagttgc actgcatttt ctttttcttt ttgagccaaa
     1021 tccaaacgtt ttttaacttc ctcttcaaat tggtggtcgc gcacttgttt taagatagaa
     1081 gcatatcctg cctggtccac cttaaaagct ttgtggcagt tgggacaaat aatttcgttg
     1141 ctgttattta tttcttgtga catgttttat gttaattaga ttaggaaact tgttgtaata
     1201 tttgagttta aatttaagaa agattttgaa ttaatctaca aaaaaacaaa ggccgctcaa
     1261 aaattgaaca gcctttgcga atcaaaattg ttgaaattta ttttttcaat aatttttctc
     1321 ttttcttatc aattttcaat tgactttcat ccaattttgc gtggcttaag tccaattttg
     1381 ccatttttgt ttgaatttta gctgcatcgc cttttccttt atctaattgg ctttgtaact
     1441 tttgctgttg tttgttcagc ttatcacgat tagcgttcaa cttagcttgt tgctcatcta
     1501 atttttcaat gtttgcttgc aatttttcag cttgagcttt tgctttttct gcggctttcg
     1561 cttctttttc tgcttcttta gctgcttttt cagcttcttt tttagctttt tctgctgctt
     1621 tttgttcttt agctgcttct tttttcgctt tttctgctgc ttttaatcgc tcagcctctg
     1681 aagcggcagc ttctttttga gcttttgctt gcgcttgtgc ttctttcaat ttagcttctt
     1741 gctctttttg ttctctttta gcatcttctg cagttaactt cgcttcttta gatgcatcta
     1801 caactttgtt ttcagcattt ttttgagctt gttcttgttg tttttttgct gcttcttctc
     1861 ttgctgcttt ttctgctgct aagcgttctt ccatttcttt gcgttgcttg tcaagagctt
     1921 cttgttgtgc ttttgcttct gctgcttttt tctcagccgc tgcttttgtt gcttcagcct
     1981 cgctttgcgt ttgctgcgtt gattgtttta tagcatcttc ttgtttttta gcttcttcgg
     2041 ctgctttatt tgctgcttct tgttttgctt tgtattccgc ctcacgcgct gctgcatcat
     2101 caatagattc tgttttagac tttaatacaa atggaataat tccatcgttt tctaatacaa
     2161 cttctgattt actatcgttt ctatataatg tatgcgatac gtcaatacgc accaattgtt
     2221 tgttgtacga agcaattgta attttagcat ttcctgccgc atctgttgtt ccagctccta
     2281 ataccgtgtt gccaagggta accttaacta aagctccaac aactggacct ttatcacttt
     2341 tcacattaac tgtaaacgta actcctgctt ttgaaatttc ttgcttagtt gttgtttgta
     2401 cattattttc agcgtatgca aaaatacctg aagtagcaat aaataatgac aaaataagta
     2461 ctctcttttt cattttactt gatttatttt aaacttaaag ttaaacaaat ttattaacaa
     2521 atttaccttt ttaacaaata ctattccaca aataattaat tttacttcat gcaattttca
     2581 gaagtaattg gtcaacatca tttaaagcag ctattaatta acgaaatcaa tagcaaaaaa
     2641 ataagtcacg caaagctttt tttaggaaag gaaggacatg gtgttttgcc tttggctttg
     2701 gcgtatgtgc aatatttatt ttgtaaaaac aaacaagaaa atgacagttg cggaacgtgt
     2761 gattcttgtc taaaagttca aaatttacag catccagacc ttcattttat ttatccgatt
     2821 gctttagctg aaaaaaaatc gtgcgcagac ctttatccta aatggcgtag taagataaaa
     2881 gaaaatccat atttctcctt agaagattgg accaatttca tcgatccaaa aggaagaagg
     2941 ctaattatag gtgtagatgc aagtacagaa attttaaaaa gtttaacact taaagctttt
     3001 gaaggtcgcc aacgtgttgt tgtaatttgg atggctgagg aaatgaatga ttctgccgct
     3061 aataaaattt tgaaagtttt ggaagaacct cctttgggca cttattttat tttagtgggg
     3121 cgccaacacg ataaattcct taccaccatt ttatcacgca cacaattcta tcgagttccc
     3181 aaaattgaaa ccactgattt aagcatacac ttgcgcgaag catttgaact caacatttca
     3241 aaagctgatt ccatcacgct gcgttcagaa ggagattatt tggaagcggt acaaatgtta
     3301 catgattcta gtgatattaa cgaagaacgt gaattgttta tcgaattaat gcgctcgtgc
     3361 tataaaaaag acgtaaacaa aatg
//
LOCUS       DAOQVQ010000245         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32129_length_3382_cov_3.306282, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000245 DAOQVQ010000000
VERSION     DAOQVQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32129_length_3382_cov_3.306282"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2837)
                     /locus_tag="PKN22_07690"
     CDS             complement(<1..2837)
                     /locus_tag="PKN22_07690"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS42625.1"
                     /translation="MRLILLLASIIWCFSTFSQRYFFKTYSTPDGLAQSQVASICQDS
                     MGYLWIGTFGGVSRYNGNNFTNFSTDDGLLNNRINVVKWINAQLFIGHQGGISIYKNN
                     KISKFPLSPDNFNTNVTDIVLFNNKIIISTNGDGLYIFENGKLKPLKLLSDEGNFIRD
                     LLVWNDKLYIATRNGVVVTSNLNTVDALIADGFSISSLTTYNNQLVVSTFYDGIQFYK
                     NHQLVHSILENDELLINYIATDSKNNLWICSKNGLIKYANKTNTFYTTAIGLPINVIS
                     TIFEDSDQNIWIGTQGKGLVKAGVDAISYFDKSTGLPSDIVLCGVQNKKGDYFFGSID
                     NGIFKTRDFKNYTIFPTTTSIWSAIADVDGKSWFGTKDGLYAIDDNNKVSIQHFNDEA
                     PGYKITAFHKIDANRMYVGGSHGIALYEKGKFTPIGSNHQQIGTIRSITLRNQQLLVG
                     TDIGLYFLDNQNKFQLIGNINHATLSLVKTVSNRIFFGSEDGLFELLKNNSIVRVRFS
                     TDVASNYVNFLSSHTHNLIVGTNNGVYIIDTQNGSKFTQRIGASEGLLDPETNLNSSF
                     IDKKNNLWFGTASALIKFDLNKFNLSVGKVKLVLTSILLDYKPFDYTQFGAKMQDEIP
                     QNMSFSYQKNNLQFNLDAVALSDFDNLLFQFKVNGLNDAWMPASKTSHIILNELAPGD
                     YKLHARVMLENGKILDQLTIKFTIRQAFYKTWWFICLAVAFLAAMVFWWIKTKIKREQ
                     EKYRLEQAEMQSRLVLLEQESLNASMNRHFIFNSLNSIQYFINISDKLSANKYLSNFA
                     KLIRKNLDTSSEHSSLVGLNEEIERIRLYLSLEAMRFNEKFTYTISTNNVDLESTMIP
                     AMMIQPFVENSIIHGILPQTDRKGEISIDVNESDSYLTILIKDNGIGIEQSIHQKSNI
                     SGDHQSKGMEITTKRVKLMRQLLSKNMEIIGP"
     gene            complement(2834..>3382)
                     /locus_tag="PKN22_07695"
     CDS             complement(2834..>3382)
                     /locus_tag="PKN22_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS42626.1"
                     /translation="FQVVFVTAFKDYAIKAFNASAVYYLLKPIDIEELQLAVKKLVES
                     KEIYTENPSNYSTYFDSLKNLSYNLLRNKPNNRIAISHTKGLKIVEDDTILHLEASGN
                     CTTIYFKDGTRYLDTRTLKTYEEMLDESKFARIHKSHIINLNELSEYLNEEGHFAILK
                     NGLKLPIARNRVTDFIKMLKQK"
BASE COUNT         1056 a          557 c          604 g         1165 t
ORIGIN      
        1 ggcccaataa tttccatgtt tttacttaat aattgacgca tcaacttaac gcgtttggta
       61 gttatttcca ttcctttgga ctgatgatcg cctgaaatat tgcttttttg atggatgctt
      121 tgttcaatgc ctataccatt atcctttatt aaaatggtta aataggagtc tgattcattg
      181 acatcaatac taatttcacc ttttcggtcg gtttgaggta aaattccatg aattatgctg
      241 ttttcaacaa aaggttgaat catcattgca gggatcattg ttgattctaa atctacattg
      301 ttggtggaaa tggtgtaggt gaatttctca ttgaatcgca tggcttctag agataaatat
      361 aaacgaatgc gctcaatttc ttcgttcaac ccaaccaaac ttgaatgttc ggaagatgta
      421 tcgagatttt ttcgaattaa tttggcaaag ttggaaaggt atttattagc cgataattta
      481 tcggatatat taataaagta ttgaatactg tttaaggaat tgaaaataaa atggcggttc
      541 atactggcat tcagcgattc ttgttccaat agtactaaac gcgactgcat ttcggcttgt
      601 tctaaacggt atttttcttg ctcgcgctta atttttgttt ttatccacca aaataccata
      661 gcagcaagaa atgcaactgc taaacaaata aaccaccaag ttttgtaaaa tgcttgacga
      721 atggtgaatt ttatggtaag ttgatcgagg attttcccat tttcaagcat aacacgagcg
      781 tgcaatttgt aatctccagg cgccaattca tttaatataa tgtgtgatgt tttggaagca
      841 ggcatccaag catcgttaag tccattcacc ttaaattgaa acagtaaatt gtcaaaatca
      901 cttagtgcta ctgcgtctaa attgaattgt aaattatttt tttgatagga aaaactcatg
      961 ttttgcggaa tttcatcctg catttttgct ccaaattgag tgtagtcaaa tggcttgtaa
     1021 tccaataaaa tcgaagtgag caccaatttt actttgccaa ctgaaaggtt gaatttattg
     1081 aggtcaaatt tgattaaggc agaggcagtt ccaaaccaaa ggttattctt tttgtcaata
     1141 aaacttgaat taagattggt ttctggatca agtagtcctt ctgaagcacc aatgcgttgg
     1201 gtaaatttgc tgccattttg agtgtcaatt atgtaaacac cgttattggt tccaacaatt
     1261 agattgtgcg tgtgactgga taaaaagtta acataattgg atgccacatc tgttgagaat
     1321 cgaacgcgaa caatggaatt attcttcagt aactcaaaca aaccatcttc tgaaccaaaa
     1381 aagatgcgat tggaaactgt ttttacgagc gatagcgtag cgtgattgat atttcctatt
     1441 aattgaaact tattttgatt atctagaaaa tacaacccaa tatctgttcc aaccaaaagt
     1501 tgctgattac gcaatgttat acttcgtatt gttccaattt gttgatgatt tgacccgatt
     1561 ggagtaaatt tacccttttc gtataaagca ataccgtgtg aacctccaac atacatgcgg
     1621 ttggcatcga ttttatgaaa cgcagtaatt ttataaccgg gtgcttcgtc gttgaaatgt
     1681 tgaatactta ccttgttgtt atcgtcaata gcgtataagc catcttttgt accaaaccac
     1741 gattttccat ccacatcagc aatagcactc caaatggatg tggttgttgg gaagatggtg
     1801 tagtttttaa aatcacgtgt tttgaaaata ccgttgtcaa tggagccaaa aaaatagtcg
     1861 ccttttttat tttgtacacc acacaaaaca atgtcagaag gaagccctgt agatttatca
     1921 aaatacgaaa tagcgtcaac acctgctttt accaaccctt ttccttgtgt tcctatccaa
     1981 atattttggt cagaatcttc aaaaatagta ctaataacat taataggtaa tccaattgct
     2041 gttgtataaa aggtgtttgt tttgttggcg tatttgatta agccattctt actgcaaatc
     2101 cataaattat ttttagaatc ggttgcaatg taattaatca acaactcatc gttttctaaa
     2161 atagaatgaa ctagctggtg atttttgtaa aattgaatgc cgtcataaaa agtgctaacc
     2221 accaattgat tgttataggt ggtgagagaa gagatgctaa acccatcagc aattagtgca
     2281 tcaacggtgt ttaaattact tgtaacaact acgccatttc gagtggcaat gtataattta
     2341 tcgttccaaa caagcaaatc acgtataaaa tttccttcgt ctgacaataa tttgaggggc
     2401 tttaattttc cattttcaaa aatatataag ccatcgccgt tggtagaaat aattatttta
     2461 ttgttgaata aaacgatatc ggtaacgttg gtgttgaaat tatcgggact taaagggaat
     2521 tttgaaattt tattgttttt ataaatagaa attccacctt gatgccctat gaataattgt
     2581 gcgtttatcc atttcaccac attaatgcgg ttgttgagca agccgtcgtc ggttgaaaaa
     2641 ttcgtaaaat tattaccgtt atagcgcgat acgccaccaa aagtgcctat ccacaaatag
     2701 cccatgctgt cttgacaaat tgacgcaact tgcgattgtg ccaaaccatc tggggtggag
     2761 taggttttga aaaaatagcg ttgtgaaaat gttgaaaaac accaaataat actcgctaat
     2821 aacagtatta gtctcatttt tgtttaagca ttttgatgaa atctgttact ctgttacgtg
     2881 caattggcaa ttttaatcca ttttttagaa tggcaaaatg gccttcttcg ttaaggtatt
     2941 ctgagagttc gtttaaattt attatgtgcg acttgtgaat gcgggcaaat tttgattcat
     3001 ccaacatttc ttcatacgtt tttagagtgc gtgtatctaa atagcgtgta ccgtctttaa
     3061 aataaatggt tgtacagtta ccagatgctt ctaaatgcaa aatggtgtcg tcttctacaa
     3121 tttttaatcc ttttgtatgc gaaatggcaa ttctattgtt gggtttgtta cgtaataaat
     3181 tgtaggataa atttttaagc gaatcaaaat aagtagaata gttagaagga ttttcagtat
     3241 aaatttcttt cgattccact aattttttaa cggccaattg caattcttca atatcaattg
     3301 gtttaagtaa ataatatacg gcacttgcgt taaatgcttt gattgcataa tctttaaaag
     3361 cagtaacaaa taccacttgg aa
//
LOCUS       DAOQVQ010000246         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32143_length_3381_cov_2.875526, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000246 DAOQVQ010000000
VERSION     DAOQVQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32143_length_3381_cov_2.875526"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKN22_07700"
     CDS             complement(<1..522)
                     /locus_tag="PKN22_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS42627.1"
                     /translation="MSKELKSTEFGLEKIFEGAQDFLPIMGTDYVEFYVGNAKQAAHY
                     YKTAFGFQSEAYAGLETGVRDRASYVLKQDKIRLVLTTALNSNSPIGEHVKKHGDGVK
                     VVALWVEDARYSYEETVKRGAKSFMEPVVEKDENGEVVRAGIYAYGETVFIFVERKNY
                     NGLFLPGYKKWESS"
     gene            complement(555..1715)
                     /locus_tag="PKN22_07705"
     CDS             complement(555..1715)
                     /locus_tag="PKN22_07705"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNS42628.1"
                     /translation="MPVYHKLGKIPPKRHIQFRKDDGNLYYEELFCTAGFAGVYTNMY
                     HIHRPTMIKSYGKAYSVAPKIVDKNNVKPYLLEGFQVPPTPDFLESRKIVFANSSTYV
                     VLAAPTNKTMDYFYKNTGADELIFIHKGSGKLRTFLGNLDFGYGDYLIIPRGTIYQIE
                     FDTDDNRLLIVESKDPFFTPKRYRNNFGQLLEHSPFCERDFRFPEELESHDELGDFLM
                     KTKKNDEIIEVVFAAHPFDVIGYDGYNFPYAFNIKDFEPITGRIHMPPTIHQHFETAS
                     SVICSFVPRLFDYHPQAIPAPYNHSNIDSDELLYYVEGKFMSRPHVKAGNITLHPAGF
                     THGPQPGAAEASIGKKDTEEYAVMVDTFAPLMVTEEAIKIADPEYYKTWLDN"
     gene            1936..2226
                     /locus_tag="PKN22_07710"
     CDS             1936..2226
                     /locus_tag="PKN22_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42629.1"
                     /translation="MKFKLFIVGALVLASCSPKTTADSKEKSMEFPNAKVEEGYGLYA
                     QHCVKCHKWKKVDNFTREQWDKILPAMAKKAKISAELEATINEYVNWELSKK"
     gene            2294..3355
                     /locus_tag="PKN22_07715"
     CDS             2294..3355
                     /locus_tag="PKN22_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNS42630.1"
                     /translation="MDLKELVIIDASTVLAGPSVGMFFAELGAKVIKVEHPLHGDVTR
                     KWKLPTEDKNSSVSAYFSAVNYKKEYQKLDYTVEADYNQFLKLIEQADILITNFKKGD
                     AEKFNLTDDVLFAKNPQLIHGKISGFGKHSDRVAYDLIVQAESGFMSMNGTPESGPVK
                     MPVALIDVLTAHQLKEGLLVALINRISTNKSYVVEASLYESAVASLINQATNYLHEKF
                     IPQRIGSLHPNIAPYGEIFKTKDQHYITFAIGSNTHFEKLCSFLNLSELIKDARFSDN
                     QSRVINRFLLKDILQAKIELVAADEILEAMHRLFVPVGKIKALDEVLNDKNTQHLVLE
                     EQIDGVPTKRLKSVVFHLN"
BASE COUNT         1108 a          594 c          588 g         1091 t
ORIGIN      
        1 ggatgattcc cattttttat atcctggcaa gaacaaaccg ttgtaatttt ttctttctac
       61 aaaaatgaaa actgtttcac cataagcata gatacctgca cgaacaactt caccattttc
      121 gtctttttca acaactggct ccatgaacga tttagcacct cttttcactg tttcttcata
      181 cgaataacgt gcatcttcaa cccataaagc aacaactttt acaccgtctc cgtgtttttt
      241 aacgtgctca ccaattggag aattagaatt caacgctgtt gttaaaacca aacgaatttt
      301 atcttgtttt aatacatacg aagcgcgatc tcttacacct gtttccaaac ctgcataagc
      361 ttcagactgg aatccaaatg cagttttgta ataatgcgcc gcttgttttg catttccaac
      421 atagaactca acgtaatctg ttcccatgat tggaaggaaa tcttgcgcac cttcaaaaat
      481 tttctctaaa ccaaactccg ttgattttaa ttctttactc atactctcaa cttttatatt
      541 ttaaacactc tttcttaatt atctaaccac gttttgtaat actcaggatc ggcaatttta
      601 atcgcttctt ctgttaccat caatggagca aatgtatcta ccattacagc gtattcttcc
      661 gtgtcttttt tacctatact tgcttcggca gcgccaggtt gcggaccatg tgtaaatcct
      721 gcaggatgca atgttatgtt acctgctttt acgtgtgggc gcgacatgaa ttttccttcc
      781 acataataca acaactcatc cgaatcaatg ttggaatggt tgtatggtgc cggaattgcc
      841 tgcggatggt agtcgaacaa acgaggaacg aacgaacaaa taacagacga agccgtttca
      901 aaatgctggt gaatagttgg tggcatgtga attctgcctg taattggttc aaaatcttta
      961 atattgaacg catacgggaa gttgtaacca tcatatccaa tcacatcaaa tggatgagct
     1021 gcaaaaacaa cctcaattat ttcgtcattt ttctttgttt tcatcaagaa atcacccaat
     1081 tcatcatgtg attctaactc ttcaggaaaa cggaaatcgc gttcgcaaaa tggcgaatgt
     1141 tctaacaatt gtccaaaatt atttctgtaa cgttttggcg taaaaaacgg atcttttgat
     1201 tcaacaatca acaaacggtt gtcgtctgta tcaaattcta tttggtaaat ggtgcctcgt
     1261 ggaataatta aataatcgcc ataaccaaag tctaaattcc ctaaaaatgt acgtaattta
     1321 cctgaacctt tgtgaatgaa aattaattca tcagctcccg tgttcttgta aaaataatcc
     1381 attgttttgt tcgtcggcgc tgcaagcaca acataagttg aactattagc aaaaactatc
     1441 ttacgcgatt ctaagaagtc gggtgttggt ggaacttgaa aaccttctaa taaatatggt
     1501 tttacgttgt tcttatctac aatttttgga gcaactgaat aagcttttcc ataactttta
     1561 atcatcgttg ggcgatgaat gtggtacatg ttggtgtaca ctccagcaaa tcctgcagta
     1621 caaaataact cttcatagta taaattgcca tcgtcttttc ggaactgaat gtgtcgtttt
     1681 ggtggaattt tccccaattt gtgatataca ggcatttttt agatttttat agtttattag
     1741 atttcttttt atttcaaaac aaatataact aaaaataaaa gcgctgattc gtgaatgtgt
     1801 aattattaac aaagattttg ggttttgtga taatataaaa aaacaactgc tagagcaatt
     1861 atcaaatttc ttaatatttc ttaagattta ttgaactcaa ttattttttg taatttagca
     1921 gaaatgaaca actttatgaa atttaaatta tttatcgtag gagctttagt attagcttca
     1981 tgtagtccta aaacaacagc agactcaaaa gaaaaatcga tggaatttcc aaatgcaaaa
     2041 gtggaagaag gatatggatt gtatgctcaa cattgtgtaa aatgccacaa atggaaaaaa
     2101 gtggataatt ttacacgtga acagtgggat aaaattcttc ctgcaatggc taaaaaagct
     2161 aaaatttctg ctgaattgga agcaacgatt aacgagtatg ttaactggga gttaagcaaa
     2221 aagtaatgaa ttaaaaaaat gaaaagaggt aactaaagga ttgaaagttg cctctttttt
     2281 ttatgaaata gagatggatt taaaagaatt ggtaattatt gatgcttcta ccgttttggc
     2341 tggaccatca gtagggatgt tttttgcaga attaggtgcc aaagttataa aagtagaaca
     2401 cccactacat ggcgatgtta ctcgtaaatg gaaattgcct acagaggata aaaacagttc
     2461 tgtttctgcc tatttttcgg ctgtcaacta caaaaaagaa tatcaaaaat tagattatac
     2521 agttgaagca gattataatc aatttttaaa gttaattgaa caagccgata ttttaattac
     2581 caattttaaa aaaggtgatg ctgaaaaatt taacctaacg gatgacgtgc tttttgcaaa
     2641 aaatccccaa ctcattcacg gaaaaatttc tggttttggc aaacattctg acagggtggc
     2701 ttatgactta attgtgcaag cagaatcagg ttttatgagt atgaatggaa caccagaaag
     2761 tggtcctgtt aaaatgcccg ttgctttaat tgatgtgttg actgctcacc aattaaaaga
     2821 aggtttatta gtagctttaa ttaacagaat atcaacaaat aagagttatg ttgttgaagc
     2881 ttcactttat gaatcagcag tggcaagttt aatcaaccaa gcaaccaatt atttgcacga
     2941 aaaattcata ccgcaacgca tcggaagctt gcatcctaac attgcacctt atggcgaaat
     3001 ttttaagaca aaagaccaac actacatcac atttgccatt ggaagcaaca cacattttga
     3061 aaagttgtgt agctttttaa atttgtcgga gttgattaaa gacgctcgtt tttctgacaa
     3121 tcaatcgcga gtgattaatc gttttttact aaaagatatc ttgcaagcta aaattgaact
     3181 tgttgctgcc gatgaaattt tagaagctat gcaccgattg tttgtcccag ttggaaaaat
     3241 caaagcattg gatgaagtgt tgaacgataa aaacacccaa catttggtgc ttgaagagca
     3301 aattgatggt gtgcctacca aacggttgaa atcagtggtt tttcatttga attagatgga
     3361 atttagagcg ccacaaacag c
//
LOCUS       DAOQVQ010000247         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32200_length_3377_cov_3.685430, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000247 DAOQVQ010000000
VERSION     DAOQVQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32200_length_3377_cov_3.685430"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..839
                     /gene="folE"
                     /locus_tag="PKN22_07720"
     CDS             246..839
                     /gene="folE"
                     /locus_tag="PKN22_07720"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNS42631.1"
                     /translation="MKESIQYNDKVTENISEHYVDILKQIGEDPQREGLLKTPERVAK
                     ALQFLTHGYDLNPDEIMQEAIFHENYSEMVIVKDIEVYSMCEHHMLPFFGKAHVAYIP
                     KGKIVGLSKIPRIVDAYARRLQVQERLTLEIRDCIKRTLDPVGVAVVLECSHMCMQMR
                     GVEKQNSTTTTSAFTGIFLNNDSTRKEFINLVQANLH"
     gene            1013..1729
                     /locus_tag="PKN22_07725"
     CDS             1013..1729
                     /locus_tag="PKN22_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNS42632.1"
                     /translation="MTGFTFGPAMVIGLVFMALGLLVSFILKRKFAKYSQIPSQRGLS
                     GREVAEMMLKDNGIYDVRVISVEGQLTDHYNPMNKTVNLSHDVYNGRNAAAVAVAAHE
                     CGHAVQHAKAYAPLNWRSKLVPLQHASGMILNVIMMATIFGGAFLHSIFPVKTMLLVV
                     VIAYGAIALFSVVTLPVEFDASKRALAWIKDSGVATHQEYEQSKDALKWAATTYVVAA
                     LGAITTVLYYLSMLLGRNED"
     gene            1812..2003
                     /locus_tag="PKN22_07730"
     CDS             1812..2003
                     /locus_tag="PKN22_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42633.1"
                     /translation="MGRPPSKPARLKDGFYIEVKNPGSGTIILRRDTEQQMLLAAKEY
                     ARTKEVVVLGEMKNDKWID"
     gene            2626..>3377
                     /locus_tag="PKN22_07735"
     CDS             2626..>3377
                     /locus_tag="PKN22_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42634.1"
                     /translation="MHRFFKIFKFYQTDDIDFRLYKKREIDFKRLAILNSIVYVVVFP
                     LLLFYLNNSEISKVYYNTSIIYLFLFPFILILSWLLPAIRNKLYLFVLIFIYTFTLYI
                     FLDLVEHNFQFVDVLSFFVLYTVCIFLIQQFSHGYIYIIYVVLLTLYSFIFIDNNQPI
                     SKNLFLFYILSISFMGVFSIFLRNKTLKSINNINKYLRTISRGNKFGFFIMKIEGGYL
                     KLIDYDNDFTLSILEDVKDINSVEEITKLINA"
BASE COUNT         1158 a          444 c          507 g         1268 t
ORIGIN      
        1 tttttgggat aaaatatttg gaacttacat aaataccgat aaaaaaatca caattggtgt
       61 gaatggttac gttacaaaag gtttttggat ggatatgttt gaaggcaatc gttcttttat
      121 aagtgctgtt ttaaaattaa ttaaacccaa gaagtacaat tcctccataa agtaaacatt
      181 aaaaattttg ctaactttgt ttttttaaaa taaaaagtaa acttttaatt tatcctctaa
      241 taatcatgaa ggaatctatt caatacaacg acaaagtaac ggaaaacatt tcggagcatt
      301 acgtagatat tctaaaacaa attggcgaag atccacaacg cgaagggttg ttaaaaactc
      361 ctgaacgtgt ggctaaagcc ttgcagtttt tgacacatgg ttatgacctt aatcccgatg
      421 aaatcatgca agaagcaatt tttcatgaaa actattcgga aatggttatt gtaaaggata
      481 ttgaagttta ctcgatgtgt gaacaccaca tgttgccatt ttttggaaaa gcacacgtag
      541 cctacattcc caaaggaaaa attgttggtt tgagtaaaat tccacgcatt gtggatgctt
      601 atgcacgcag gttgcaagtg caagaacgcc taacattgga aattcgcgat tgcatcaaac
      661 gcacactaga cccagttggt gtagcagttg ttttagaatg ctcacacatg tgtatgcaaa
      721 tgcgtggggt tgaaaaacaa aactctacaa ctaccacttc agcatttaca ggtattttct
      781 taaacaacga ttctacacgt aaagaattca tcaatttggt gcaagcaaac ttgcattaat
      841 tccttgaata actatcaatt attacaatta cacatttgtt ttattgacag attgtcatac
      901 tatttttatt gattttacgc tttttgtcac tgtttttact aaaaaatgtg aaaaaacact
      961 gcatttcaag aatttgtatt ttatttgata taaatacaga aaataattga atatgacagg
     1021 atttacattt ggtccggcaa tggttatcgg actcgttttt atggcacttg gtttacttgt
     1081 aagttttata ttgaaaagaa aatttgcaaa atacagccaa attccttctc aacgtggttt
     1141 atcgggtagg gaagtggccg aaatgatgtt gaaagataat ggaatttatg atgttcgagt
     1201 gatttctgtt gaaggtcaac ttaccgacca ttacaacccg atgaacaaaa ccgtaaattt
     1261 atcgcacgat gtgtataacg gaagaaacgc agctgctgtt gcagttgcag ctcacgagtg
     1321 cggacatgct gttcagcatg caaaagctta tgcaccatta aattggcgtt caaaattggt
     1381 tccattgcaa catgctagtg gtatgatact caacgtgata atgatggcaa caatttttgg
     1441 tggtgctttt ttacattcca tttttccagt aaaaactatg ttgttagttg tagttattgc
     1501 ctatggtgcg attgctttat ttagtgtggt tacacttcca gtagagtttg atgcatccaa
     1561 acgagcattg gcatggataa aagacagtgg agttgcaact caccaagaat atgagcaatc
     1621 aaaagatgca ttaaaatggg cggctacaac ctatgtagtt gctgctctag gtgctattac
     1681 aactgttctt tactatttat ctatgttgtt gggaagaaat gaggattagt ttaatttttg
     1741 ttaatttttt ttcaatctaa ataaatttaa ctagttttat attgttagtt taaatttaaa
     1801 atcaaatagt tatgggaaga ccaccttcaa aacctgctcg cttaaaagac ggtttttata
     1861 ttgaagttaa aaacccaggt agtggaacaa ttatattacg aagagacact gaacagcaaa
     1921 tgttattagc tgcaaaagaa tatgctagaa caaaagaagt tgttgtttta ggtgaaatga
     1981 aaaacgataa atggattgat taatcacttt attttaaatt taaaagcggt tgttttgtta
     2041 ataaagcaac cgcttttgtt ttattaactt ttgtgttctt ttattcactt ttaaaaaatt
     2101 agttatttta taactagttg atttttatat ttttaaaata ggtagtttta aacgattttt
     2161 acagctaaat tgttaattac tgtttataaa attcaataaa attttctttg aaatataaaa
     2221 tacctgtata tacaagattg aaaatgaaaa atcaaaacaa gttagccatt ttttaaacac
     2281 tttttttcag caattcatta acttttttta agacgaaaat aacgataaaa taataaacat
     2341 ttggttgttt aacattgaat ggtgtaattt gttaattact ttataaatag tttattttta
     2401 aaaggttaaa ctattattat tgtaaataaa aagttaataa gtattaatat cttaataaaa
     2461 gaataaattt ttaaaaattc tattcacata ttaacaacaa taataatgaa taataaaaat
     2521 tttaaaaatt aatttttctt tatttttttt tatttatcta aaatctattt ttttctgtat
     2581 tttcgatttt ttataatata acaatcactg tttatcaatt aaataatgca tcgttttttt
     2641 aaaatattca aattctacca aacggatgat attgatttca ggctctataa aaaacgtgaa
     2701 attgatttta aacgattagc aattctaaac agtatcgtat atgttgttgt ttttccactt
     2761 ttactttttt atttaaataa ttccgaaata tcaaaagttt attataatac cagtataatt
     2821 tacttgttct tatttccatt tatcctaatt ttaagttggt tgttaccggc cattagaaat
     2881 aagctttatt tgtttgtact tatttttata tacacattta cattgtatat atttttagat
     2941 ttggtagaac ataattttca atttgtagat gtacttagtt tctttgtttt atatactgtt
     3001 tgtatttttt taattcagca gttttctcat ggctatatat acattattta tgttgtttta
     3061 ttgactttat attcctttat atttattgat aacaatcagc caatttctaa aaatttattt
     3121 ttattctata ttttaagtat ctcatttatg ggagtttttt ctatattcct tagaaataaa
     3181 accttaaaat ctattaacaa catcaataaa tatttacgca ctatttcaag aggtaacaaa
     3241 tttgggtttt ttattatgaa aattgaaggt ggttacttaa aacttataga ttatgataat
     3301 gattttactt tatccatcct tgaagatgta aaagacatta attcagttga agaaattact
     3361 aaattaatta atgctat
//
LOCUS       DAOQVQ010000248         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32217_length_3376_cov_3.593797, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000248 DAOQVQ010000000
VERSION     DAOQVQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32217_length_3376_cov_3.593797"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKN22_07740"
     CDS             complement(<1..500)
                     /locus_tag="PKN22_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNS42635.1"
                     /translation="MRKVFITGMGSISSIGLNIAEQLSNLRACKTGIGKARHFQSIYT
                     ETLPFAEINESDEQLKQMTGYAEYGATRTTLIAIKAFQEAVENSGLTQEQLSNPKTAF
                     ISSSTVGGMCYTDALYADANNKGEPSEFFSSYEGSNHTLQLVKHFHIKGYTDTINTAC
                     SSSANA"
     gene            complement(507..776)
                     /locus_tag="PKN22_07745"
     CDS             complement(507..776)
                     /locus_tag="PKN22_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNS42636.1"
                     /translation="MEISRDELTIEFKQHLITYLNLLDTKVEDIKNDEPLFGGDLGLD
                     SIDSVEMIVLLDREYGIKIKNPTEGRQVLVDVNTIVDYIIANRTK"
     gene            complement(779..2062)
                     /locus_tag="PKN22_07750"
     CDS             complement(779..2062)
                     /locus_tag="PKN22_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42637.1"
                     /translation="MRKLRATLIKELQLLVHDKVGLLLMYAMPVVLVFIITLVQDSAF
                     KIVNNNQLEIIVANHDKGTLGDTLVKMLEKSGTFIVEKNNRLSEKALQQKGITDGKLA
                     SIYIPTHFSASIENNSGNVTKLILVEFGMSEDSVKAAESTTITNLIVYFDPVLQENYQ
                     YTLKTNLQTLLSGMENQVLLRKLFKEMGYDAIPEHIASKMTGEQTAIVAKQASINGKT
                     AVVPNATQHNVPAWSIFAMFFMVVSLGGNIVKERNSGSFVRLLTIPPSFSLTLISKMV
                     LYLGVAISQLALLFAIGYWIFPFLELPALQMPGSIFQLFVVALLSGMAAVSYAVLIGT
                     YAKTQEQANGFGAISIVIFAAIGGIWVPTFVMPTYLQNIALISPLHWCLEGFYSLFLK
                     NGDWNSLSFSIIFLSLFTIICQILSFAKLKSQQYL"
     gene            complement(2059..2778)
                     /locus_tag="PKN22_07755"
     CDS             complement(2059..2778)
                     /locus_tag="PKN22_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS42638.1"
                     /translation="MAKKYKNSKVNAVDEVQLNIVEGEKLGVFGPNGAGKTSLMAMLC
                     GIVLPSSGSIYYHIDGKKLTCKDALPFIGYVPQDFAFYPELSAQQNLIYFGKMYGIPT
                     NELNQRIDLLLEKLGLSAFRYQLLSTFSGGMKRRINLAIGILNNPKVLFLDEPTVGVD
                     VQSKYAIDQLLEELNKNGTTIVFTSHHLKESEQFCDRLVLLDKGKIIANGTMEALKQQ
                     YMVTDLEQLLIQLTGKELRDS"
     gene            2959..>3376
                     /locus_tag="PKN22_07760"
     CDS             2959..>3376
                     /locus_tag="PKN22_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42639.1"
                     /translation="MIKHMILVASILCCTTGVYSQEDVKVLSQTEVEQLKTQMPLAAE
                     TAYNEGILAFQAKNYTVAAEKYSAAINAYPAFEMAFYNRGLCYEALKNNDNALADFQK
                     AVELNPNNSDALYKLSSMYDATGNKEKSEEYLNKAIA"
BASE COUNT         1084 a          639 c          591 g         1062 t
ORIGIN      
        1 atagcattgg ccgatgaaga acaagcagta ttgatagtgt cggtataacc ttttatatga
       61 aaatgtttga caagttgcaa agtgtggttg gaaccctcat aagatgaaaa aaattctgac
      121 ggttcacctt tgttatttgc atccgcataa agagcatcag tatagcacat gcccccaact
      181 gttgaagagg aaataaatgc tgtcttggga ttgcttagct gttcttgcgt aagaccgcta
      241 ttctcaaccg cttcttgaaa tgctttgatg gctattaacg tggtgcgagt ggcaccatat
      301 tctgcatatc ccgtcatttg tttaagctgt tcatcacttt cgttgatttc agcaaaaggc
      361 aatgtttcgg tatatatgga ttgaaaatga cgtgcttttc caattcctgt tttgcaggca
      421 cgcaaatttg ataattgctc ggctatgttt aaaccaatag aagatattga acccattccg
      481 gtgataaata cctttctcat agcggtttat ttggtgcgat tggcaatgat ataatcaact
      541 atggtgttga catccactaa aacttggcgt ccctcagttg ggtttttaat tttaatgccg
      601 tattctctat ccaacaaaac aatcatttcc acagaatcaa ttgaatctaa acccaaatca
      661 cctccaaata atggttcatc gtttttaatg tcctcaactt tggtgtccaa caagttcaaa
      721 taagtgatta aatgttgctt aaattcaata gttaattcgt ctcttgatat ttccataatt
      781 ataaatattg ttgtgatttt aatttggcaa aactaagtat ttgacaaatt attgtaaaca
      841 aagaaagaaa aattatactg aaactcaatg aattccagtc gccatttttt agaaataagg
      901 aataaaagcc ttctaaacac cagtgtaacg gggaaataag tgcgatattc tgtaaataag
      961 ttggcatcac aaaagttggt acccaaattc cgccaattgc agcaaaaata actatggaaa
     1021 ttgctccaaa accattggct tgttcttgcg ttttagcata tgttccaatc aaaacggcat
     1081 aactcacagc ggccattcca gataaaagcg caacaacaaa taactgaaaa atagaacctg
     1141 gcatttgcaa agcaggtaac tccaaaaaag gaaaaatcca ataaccaatg gcaaacaaaa
     1201 gtgctaactg actaatggca acccctaaat acaacaccat tttgctgatt agggtcaatg
     1261 aaaaactggg tggtatggtt agcaaacgca caaaacttcc tgagttgcgt tcttttacaa
     1321 tattgccccc caaagaaaca accataaaaa acattgcaaa aattgaccaa gccggaacgt
     1381 tgtgttgtgt ggcgtttgga accacagctg ttttgccgtt gatagatgct tgcttggcaa
     1441 caatagccgt ttgttcgcct gtcattttac ttgcaatgtg ctctggaatt gcatcatagc
     1501 ccatttcttt aaacaacttg cgcaacaata cttgattttc catcccagaa agtagcgttt
     1561 gcaagttggt ttttagtgtg tattgataat tttcttgcaa tacggggtcg aaataaacaa
     1621 ttagattagt tatggtggtg ctttcggctg ccttcacaga atcttcactc atgccaaatt
     1681 caactaaaat aagtttggta acgttgcccg aattgttttc aatagatgct gaaaaatggg
     1741 tcggaatata aatactagcc aattttccgt cggtgatgcc tttttgttgc aacgcttttt
     1801 cggataaacg attgtttttt tcaacgataa acgttcccga tttttccaac attttcacaa
     1861 gtgtatcacc caatgtaccc ttgtcatgat tggcaactat aatttccaat tgattattgt
     1921 tcacaatttt aaaggcgcta tcctgcacca gcgtaatgat aaaaacaagc acaacaggca
     1981 tggcatacat caaaagcaag cccactttgt cgtgtaccaa caattgcagt tcttttatta
     2041 atgttgccct caattttctc atgaatcgcg caattctttt cctgttaatt gaatcaataa
     2101 ttgttctaaa tcagtcacca tgtattgttg ttttaatgct tccatagttc cgttggcaat
     2161 gatttttccc ttatcgagca aaaccaaccg atcacaaaac tgttcagatt ctttcaaatg
     2221 gtgcgaagta aaaacgatag ttgttccatt tttattcagt tcttccaaca attggtcaat
     2281 cgcatatttg ctttgcacat ccaccccaac agttggctcg tctaaaaaca acactttagg
     2341 attgtttaat attccaatgg ctaaattaat tctgcgtttc attccaccag aaaaagtaga
     2401 aagcaattgg tagcgaaatg ctgaaaggcc taatttttct agtaacaagt caatgcgttg
     2461 attcaattca ttggttggaa ttccatacat tttaccgaaa taaatcaaat tttgttgtgc
     2521 cgataactcc ggataaaatg caaaatcttg aggcacatat ccaatgaagg gcaatgcgtc
     2581 tttgcaggtg agtttttttc catcaatgtg atagtaaata ctgcccgagg atggtaatac
     2641 gataccacac aacatagcca ttaaactggt ttttccagct ccgtttggac caaatacacc
     2701 tagtttttca ccttcaacaa tgttcaattg cacttcatca acagcgttta ccttactgtt
     2761 tttgtacttt ttggccaatg cttgtatgtg gataatgtct tgcaagtgaa ataaaaaagc
     2821 gaagataata atatttaaaa aaggtgtgtt taaattttgg aatggagaat tgaatttgta
     2881 gaaaatttca aaaataacca ccaacttttc aatgggttga tttttttatt atttttgatt
     2941 gaatacaaaa cgctatttat gataaaacat atgattttgg tggcttctat tttatgttgc
     3001 acaacaggag tctatagcca agaagacgtc aaagtacttt ctcaaacaga agttgaacaa
     3061 ctcaaaacac aaatgccttt ggctgctgaa acagcctata acgaagggat tttagcattt
     3121 caagcaaaaa attacacggt tgctgcagaa aaatattcgg cagctatcaa tgcctatcca
     3181 gcgtttgaaa tggcatttta caaccgtggt ttgtgttacg aggcactcaa aaataacgat
     3241 aacgccttag ctgattttca aaaagcagtt gaattaaatc ccaataattc cgatgcgctg
     3301 tataaattga gttccatgta tgatgccact ggaaataaag aaaaatcaga agaatatttg
     3361 aacaaagcaa ttgccg
//
LOCUS       DAOQVQ010000249         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32229_length_3375_cov_3.721386, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000249 DAOQVQ010000000
VERSION     DAOQVQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32229_length_3375_cov_3.721386"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1067
                     /gene="mnmH"
                     /locus_tag="PKN22_07765"
     CDS             15..1067
                     /gene="mnmH"
                     /locus_tag="PKN22_07765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNS42640.1"
                     /translation="MAIQALPIEEFIELSNNNLVIDVRSPSEFNYAHFPQAYSIPLFD
                     DEQRKVVGTAYKQISRQEAIKIGLDYFGIKMRSVVEQVEQLVQASNASCVLVHCWRGG
                     MRSQAVAWLLDLYGFKVFTLKGGYKAYRNWVLQQFQNKHKLLILSGFTGSGKTEILHE
                     LSNLGHPTIDLEHLSQHKGSAFGALGMPAQPSNEQFENLIALHLHQFKNSTLPIVLES
                     ENPRIGNVLIPRHFFEQMKNAPCLRIVVPVDKRLEKIVSDYGQFETEDLKASVQRISK
                     RLGGLETKRVLNFLDENNIQAAFELLIAYYDRNYLLHTPFQQPFGELELQTTNAHTNA
                     QIISDFLNRSTCVKTK"
     gene            1049..2107
                     /gene="selD"
                     /locus_tag="PKN22_07770"
     CDS             1049..2107
                     /gene="selD"
                     /locus_tag="PKN22_07770"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNS42641.1"
                     /translation="MCENEIKLTKYAHGAGCGCKIAPQVLNEILKENTSLSVSCDKLL
                     VGNSSKDDAAVYQLNETEALISTTDFFTPIVDDPFIFGQVAAANAISDVYAMGGTPLF
                     ALAILGWPIDKLPTAVANKVMEGAKSICKEANIPLAGGHSIDSPEPIFGLSVNGLVAL
                     KNLKQNNTGQIGDVLLLTKPLGVGVLATATKREQISATDLELLHKTITQLNKIGQKIG
                     QIEGVHALTDITGFGLTGHLIEMCEGAQLSATIEYATLPKIEGLSTYLSKNTIPDATY
                     RNWKAYSDKIKINNPAIGLEAFQLLPDPQTNGGLLIAVAPESLSELQQLLVQEGYADF
                     IQPIGRFTEKKEFCITVE"
     gene            complement(2176..2751)
                     /locus_tag="PKN22_07775"
     CDS             complement(2176..2751)
                     /locus_tag="PKN22_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNS42642.1"
                     /translation="MKKVILSLFVAVSTFVSAQTLTNDAFHSRLGFEVPHLMVSNISG
                     HFSDFKSTVTFTKEDLSDAKFTLTAKVATINTGVEPRDNHLRSADFFDAEKYPEMKFV
                     STSVTKEFGNKYKMEGDLTMHGVTKKVSLTVIYNGLVTNPMNKVKTHGFTVKGSLNRK
                     DFNVGANFNNAVVGETIQIVSNLEFPVEAGK"
     gene            3059..3211
                     /locus_tag="PKN22_07780"
     CDS             3059..3211
                     /locus_tag="PKN22_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42643.1"
                     /translation="MKKKANTVRLASLHSVNKKFKNKELSSFDTALQNLGVVNQSIKN
                     KTKVEI"
BASE COUNT         1125 a          568 c          628 g         1054 t
ORIGIN      
        1 aatagatttg caacatggca atacaagcac taccaattga agaatttatt gagttatcaa
       61 ataataattt ggtgattgat gtgcgcagtc cgtcggaatt caattacgcc cattttccgc
      121 aagcatactc aattccgctt tttgatgatg agcaacgtaa agttgtgggt actgcttaca
      181 aacaaattag tcgacaagaa gccataaaaa ttggtttgga ttactttgga attaaaatgc
      241 gaagtgttgt ggaacaagtt gaacaattgg tacaagcttc caacgcatcg tgtgttttag
      301 tgcattgctg gcgtggtggt atgcgaagtc aagcggttgc gtggttgttg gatttatatg
      361 gttttaaagt ttttacactc aaaggaggtt acaaagcgta tcgaaattgg gtactgcaac
      421 aatttcaaaa caaacacaaa ttgttaattc tcagtgggtt tacaggttct ggaaaaacgg
      481 aaattttaca tgagttatct aatttgggac atccaaccat tgatttagaa cacctttcac
      541 agcacaaagg ttctgctttt ggagcattgg gaatgcctgc acaacctagc aatgagcagt
      601 ttgaaaattt aattgcccta catctgcatc aatttaaaaa tagtactctg cctattgttt
      661 tagaaagcga aaatccacga attggcaacg ttttaattcc acgtcatttt tttgagcaaa
      721 tgaaaaatgc tccttgtttg cgtattgttg tgcctgttga caagcgattg gagaaaattg
      781 taagtgatta tggacaattt gaaaccgaag atttaaaagc gtctgttcaa cgaatttcaa
      841 aacgattggg tggattggaa accaaacgtg tgttgaattt cctggatgaa aacaacattc
      901 aagcagcatt tgagttactg attgcttatt atgatcgcaa ttatttgttg cacacccctt
      961 ttcagcaacc ttttggtgag ttggagttac agacaaccaa tgcgcacacc aatgctcaaa
     1021 ttatttctga ttttttaaac cgttcaacat gtgtgaaaac gaaataaaat taaccaaata
     1081 tgctcacgga gctggttgtg gttgcaaaat tgcacctcaa gtattgaatg aaatactaaa
     1141 agaaaacaca tcattatcag ttagttgcga taagctattg gtaggcaata gctcaaaaga
     1201 cgatgcggct gtgtatcagt taaacgaaac agaagcgttg atttcaacta ccgatttttt
     1261 tactccaatt gtggatgatc cttttatttt cgggcaagtt gcagcagcta atgccattag
     1321 tgatgtgtat gcaatggggg gcactcctct atttgcattg gctattttag gttggccaat
     1381 agataaattg cctactgctg ttgccaacaa agtgatggaa ggtgcaaaaa gcatttgtaa
     1441 agaagctaat attcctttgg ctggggggca cagtattgat agtccagaac ctatttttgg
     1501 gttatctgtc aacgggttgg tggctttaaa aaatttaaaa caaaacaata cagggcaaat
     1561 tggcgatgta ttgcttctta caaaaccatt gggagttggc gttttggcta ctgccacaaa
     1621 aagagaacag atttcggcta cggatttaga attgttacac aaaaccatca cgcaactcaa
     1681 taaaattggg caaaaaattg gacaaattga aggagtgcat gcgttgacag acattacagg
     1741 atttgggttg acaggtcatt taattgaaat gtgcgaagga gctcaactat ctgcaacaat
     1801 tgaatatgca acgctcccaa aaattgaagg attgtcgaca tatttatcta aaaacaccat
     1861 tccagacgca acctatcgca attggaaagc atatagcgac aaaattaaaa tcaataaccc
     1921 tgcaattggg ttggaagcat ttcaactact acccgaccca caaaccaatg gtggattgct
     1981 gattgctgtt gccccagaga gtttaagtga gttgcaacaa ttgcttgtgc aagaagggta
     2041 tgcagatttc attcagccaa ttggtcgatt tacggagaaa aaggaatttt gcattactgt
     2101 tgaataaaat taaagcatca aaaaagggca acattcataa gaacattgcc cttcattaac
     2161 attaaactta aacaattatt ttccagcttc tacggggaat tctaagttgg atacaatttg
     2221 aattgtttca ccaactacag cgttgttgaa atttgctcca acattaaaat cttttctgtt
     2281 caacgaacct ttaactgtaa aaccatgtgt tttaactttg ttcattgggt tggttaccaa
     2341 accgttgtaa atcaccgtta aactcacttt tttagttact ccgtgcatgg ttaaatcacc
     2401 ttccattttg tatttgttcc caaattcttt tgttacagaa gtagaaacaa atttcatttc
     2461 tgggtatttt tccgcatcaa agaaatcagc agaacgcaag tggttatcac gaggctcaac
     2521 acctgtattg atggttgcaa ctttggcagt taatgtaaat ttagcatccg ataaatcttc
     2581 tttggtaaac gtaactgttg atttgaaatc agaaaaatgt ccgctaatgt ttgaaaccat
     2641 taagtgagga acttcaaatc ctaatcttga atggaatgca tcgttagtta atgtttgagc
     2701 tgaaacaaac gttgaaacag ctacaaataa agataaaatt acttttttca tggtatgatg
     2761 ttttttaatt tctacaaagt tacgacttcc atttgcccta ccaattgatg tatgttaaga
     2821 aaacttaaaa aataggacaa tactatctta ataaaccaat ttttaaggtt caaaaatgaa
     2881 tagaattttt gattattcat ttttaaaatc aattcaattg aaaaaaatag catttaaaac
     2941 accaaaaaag tacgtttaat aaacgagctc aataacgtga caactttaca ggcaatttag
     3001 gcgttataat agtggcacat aaattgattt taccattaaa agctaaaact tagaaatcat
     3061 gaagaaaaaa gcaaacacag taagattggc atctcttcat agtgtaaaca agaagtttaa
     3121 aaacaaggag ttgtcatcat ttgatactgc tcttcaaaat ttaggagtag taaatcaatc
     3181 aattaaaaat aaaacaaagg tggagattta aaagtctcca cctcctcctt cattatcttg
     3241 ttgcttttgt tgcttttgag agctttccat ttttccaaac ttataagtca gtgttaaata
     3301 aacccttctt gtttgaaaat caaatttgga agtttgtgac aaatcaccac tgttcaattt
     3361 cattctaaag ttttt
//
LOCUS       DAOQVQ010000250         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32417_length_3360_cov_2.527080, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000250 DAOQVQ010000000
VERSION     DAOQVQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32417_length_3360_cov_2.527080"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKN22_07785"
     CDS             <1..308
                     /locus_tag="PKN22_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42644.1"
                     /translation="YRTMLCYLYGVDLEMRVTHKGITPDKCINLISDVGKKAGFFGKM
                     YANKTLNRLEEGLNILDAFILGVCEQTNYQIDEVMQFNVAKLRERHNNGKFDASYVQ"
     gene            432..>3360
                     /locus_tag="PKN22_07790"
     CDS             432..>3360
                     /locus_tag="PKN22_07790"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HNS42645.1"
                     /translation="MTQIKYDIPDYNIFKEWRPEQHFQRIDKYSNFLHDEGRLETWEE
                     VVERTVDALRLISKDKLLDEEYQTIFDMIYKLEIMPSMRLLSMPLKAIERCNTVLYNC
                     TYGLGDTFQSIAEAQYLSMSGCGVSWSVERKNVDKLPKIPQQTGMMYYHNVEDSQIGW
                     AKSTETLFNAWLKGNDITFQYDRIRPYGARLKTKGGYASGPQILIDTHNFIRQTLKNA
                     QGRKVTPLEMHDMFCYVLESGVSGGTRRCLSKDTNIITNNGLVKINEINASDYAWTPD
                     GYKKILNVFKQGKQSITRIKLSNGAFIKATENHRLAIPTNNGYDWKLVSDLNVGDKLL
                     FNGVEAEQGKNIEINIDPPGDFSKNYTKLEKDFIEINEEFAWVLGKFMADGSILHRTK
                     SGVDFKRGITIATHSDEIEQRQRIEKYFIQFGINIVHKKKKKENCYTTTIVNGQIGRT
                     FGDWKKANTSILIPHFIKESRSSVRAAFLAGLADGDGSLHNRNIVLVSTIYETLVNDV
                     VNLYASLGIAVHIRKTIRKVWKDIYSVTIKGLSNKENLVRLLSPYTLKKFYDFKLNMY
                     DYNYDDILLSNMQDKRLVPIEIVEIERQVEEIETFDIEVEENHCFFANGILSHNSAGM
                     CIFDADNLEIAQCKYDGFWNDPFHKVRANANNSAVWGDDLTQKDIDELTKQLFSTGSG
                     EPGLLKRNNAIRTSPEWRKFLHPEYTGQNPCFIEGTMVQTKNGHFPIESLVGKEVEIW
                     NGLEWTKINNFRVTGENQPVYTVTLHDGTEITATSYHTFILEGGERKELKDLVVGDKL
                     QISDAPLSHGNKKINGAYLKGFLMGDGTSTSDSVLLNLYEPKFMCEERLISSAEEIEP
                     GEVKTNAIVDVSFGDANQAKNSYRKSMRGINVRRHELYRYIIREYGLPQEIFEANYES
                     KCEFLAGLFDADGTASDTKNGFMYQYSSIHYQLLQDIQLLLKTIGINSTLRKNRESGV
                     KDFN"
BASE COUNT         1275 a          403 c          612 g         1070 t
ORIGIN      
        1 attatcgtac aatgttatgt tatctttatg gtgttgattt agaaatgcga gtaacccata
       61 agggtattac tcctgataaa tgtattaatc ttatttctga tgttggtaaa aaagctggat
      121 tttttggtaa aatgtatgcc aataaaaccc ttaatagact tgaagaagga cttaacatcc
      181 ttgatgcatt tattttagga gtttgcgaac aaactaatta tcaaatcgat gaggtaatgc
      241 aatttaatgt ggcaaaatta agagaacgtc acaacaatgg caaatttgat gcaagttatg
      301 ttcaataaaa tctaaaccaa aattgacaac ctcctagatg tagtgtataa taatttgaaa
      361 catacaaagc aacttgcttt tccctttaag caagttgctt ttttatcact aatttagtag
      421 aaagaaaaac catgacacaa attaaatatg acattcctga ttataatatc tttaaggaat
      481 ggcgacctga acaacatttt caaagaattg ataagtattc aaattttctt catgatgaag
      541 gacgtttgga aacgtgggaa gaagttgttg aaagaactgt tgatgcatta cgattaatta
      601 gtaaggataa attacttgat gaagagtatc aaactatttt tgatatgatt tataaattag
      661 aaattatgcc ttcaatgcgc ttattatcaa tgccactgaa ggcgattgaa agatgtaata
      721 cagttttata caattgtaca tacggtttag gtgatacttt tcaatcgatt gcagaagcgc
      781 aatatctttc tatgtctggt tgtggtgtat catggtcggt tgaaagaaaa aatgttgata
      841 agttgccaaa gataccgcag caaacaggaa tgatgtacta tcataatgtt gaagacagtc
      901 aaattggttg ggctaaatca acagagactt tatttaatgc ttggttaaaa ggtaatgata
      961 tcacatttca atatgataga attagacctt atggggcaag actgaagaca aaaggtggat
     1021 atgcttcagg accacagatt ctaattgata ctcataattt tattcgacaa acgttgaaaa
     1081 atgcgcaagg tagaaaagtt acaccactgg aaatgcacga tatgttttgt tatgttttgg
     1141 aaagcggtgt aagcggcgga acaagacgct gtttatcaaa agacacaaat attattacaa
     1201 ataatggctt agtaaaaata aatgaaataa acgctagtga ttatgcttgg actcctgatg
     1261 gttataaaaa gattttaaat gtatttaagc aaggaaaaca aagtattact agaattaaac
     1321 ttagtaatgg agcatttatt aaagcaacag aaaatcatcg attagcaatt cctactaata
     1381 atggatatga ttggaaatta gtatctgatt taaacgttgg agacaaatta ttatttaatg
     1441 gagtagaagc agaacaaggt aaaaatattg aaattaatat tgacccacca ggcgatttta
     1501 gtaaaaacta tacaaaatta gaaaaagatt ttattgaaat taacgaagaa tttgcatggg
     1561 ttttaggaaa atttatggca gatgggagta ttcttcatag aacaaaatca ggcgtagatt
     1621 ttaagcgtgg cattacaatt gcaactcatt ctgatgaaat tgaacaaaga cagcgtatag
     1681 aaaaatattt tattcaattt ggtattaata ttgttcataa gaaaaagaaa aaagaaaatt
     1741 gttatacaac tactattgta aatggtcaaa ttggtagaac atttggcgac tggaaaaaag
     1801 caaatacatc aatattaata ccacacttta ttaaagaatc aagatcatct gttcgtgccg
     1861 catttttagc aggtttggca gatggtgatg ggagtttgca taatagaaac attgtattag
     1921 ttagtacaat ttatgaaaca ctagtaaatg atgtcgttaa tttatatgct tctcttggta
     1981 ttgctgtaca tattagaaaa acaattcgta aagtatggaa agatatttac tcagttacta
     2041 ttaaaggatt atcaaataaa gaaaatttag tacgtttatt atcaccatat acacttaaga
     2101 aattttatga ttttaaatta aatatgtatg attataatta tgacgatatt cttttgtcca
     2161 atatgcaaga taaaagatta gttcctattg aaattgttga aatagaacga caagtagaag
     2221 aaatagaaac ttttgatatt gaagtcgaag aaaatcattg tttttttgca aatggtatcc
     2281 tttctcataa tagcgctggt atgtgtattt ttgatgcaga taatttagaa attgcacaat
     2341 gtaaatacga tggattttgg aatgatccat tccataaggt tagagctaat gcaaataata
     2401 gtgctgtttg gggtgacgat ttaacacaaa aagatattga tgaattaaca aaacaattat
     2461 tttcaacagg tagtggcgag ccaggattgc taaaaagaaa taatgcaatt agaacttcac
     2521 ctgaatggag aaaattttta catcctgaat atacgggaca aaatccttgc tttattgaag
     2581 gtacaatggt tcaaactaaa aatggtcatt ttccaattga aagtttagtt gggaaagaag
     2641 ttgaaatttg gaatggttta gaatggacaa aaataaataa tttcagagta acaggtgaaa
     2701 atcaacctgt ttataccgtt acattgcatg atggaacaga aattacagca acatcatatc
     2761 atacatttat tttggaaggt ggagaaagaa aagaattaaa agaccttgtt gtaggtgata
     2821 aattacaaat ttctgatgct cctttatcac atggtaataa aaaaattaat ggagcatatt
     2881 taaaaggatt tttgatgggt gatggtacaa gcacatcaga tagtgtatta cttaatttat
     2941 atgaaccaaa atttatgtgc gaagaacgct taattagttc tgctgaagaa attgaaccag
     3001 gagaagtaaa aacaaatgca attgtagatg tttcatttgg tgatgccaat caagctaaaa
     3061 attcttatcg taagtcaatg cgtggtatta atgtgcgacg ccatgaatta tatagatata
     3121 ttattcgtga atatggatta ccacaagaaa tttttgaagc taattatgaa agtaaatgtg
     3181 aatttttagc aggtttgttt gatgcagatg gaacagcaag tgacacaaaa aatggattta
     3241 tgtaccaata ctcatcgatt cattatcaac tattacaaga cattcaatta ctattaaaaa
     3301 caattggtat taatagtaca ttacgtaaga atagagaatc aggtgtaaaa gattttaatg
//
LOCUS       DAOQVQ010000251         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32731_length_3336_cov_3.418165, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000251 DAOQVQ010000000
VERSION     DAOQVQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32731_length_3336_cov_3.418165"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKN22_07795"
     CDS             <1..397
                     /locus_tag="PKN22_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNS42646.1"
                     /translation="ELIQLLKKNGYEPVLLGGEQEHEKNSMLAQTTGATYLGYFNLQQ
                     FINLMYQMDLIVTQVTMAMHITIALQKKIVLMNNIFNPHEFELYGRGVLVEPSKECIC
                     YFDGTCKNGNSCMKDLAPQTIFEEIKKQL"
     gene            398..1600
                     /locus_tag="PKN22_07800"
     CDS             398..1600
                     /locus_tag="PKN22_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS42647.1"
                     /translation="MNVLQLTYRVPYPATDGGAIGIYTITKGLNDNGCTVDLVAINTP
                     KHSQPADAMQHLVRNQIDVFVDTSISPFKLLTNLLFRSIPYNIERFVSKEVEKQLETI
                     LTNNTYDFIHIDGAFVAYYIDKIRQFSQAPIIVRPHNIEYVIWERLAINEKNPLKKWF
                     FAHLAKRLKRFEADYYNKADGIAAITPEDKNRLLNLNVKKPIDVVPVGVLFKSNSVQQ
                     SIQQEQDTIFSISALDWMPNIEGLNWFLENVWPLLLQKKPTVQLHIAGKSTPDWLKNA
                     TYPNTTVHGFVDDAQAFKQHYQLMLVPLLSGGGMRVKIIEGLAEGKCIISTTIGAEGV
                     AYTDKENIVIADTPTQWVDAICFYLENEEARKNIEQKAAQLAHQVYENTIVTKGYVDL
                     YNKLTSLK"
     gene            1597..2751
                     /locus_tag="PKN22_07805"
     CDS             1597..2751
                     /locus_tag="PKN22_07805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS42648.1"
                     /translation="MNNLPKLVVVLSRFPFPLEKGDKLRSYYQIKELAQHYEIHLIAL
                     SDVDVAISSKNELANYCKTITVYRLSKISVAFNSLVALLGKKPVQVGYFYNYFVARKI
                     QRQLQHLQPDHLFSQLIRTAEYTKNYMGCPKTLDYMDAFSKGFERRAEKSPWYSKWFF
                     QLEYKRLQVYERLMFDYFDKKTIISEQDKRYIFHPNRSEIVCVPNGIDASFFESRSLS
                     KKYDFVFVGNMNYAPNVEAVLYIAKEILPSFPMSQFLIAGANPHPRVKRLAEQNSNIA
                     VSGWMDDIRDAYASAKIFLAPMKTGTGMQNKLLEAMAMGIPCITSSLANNAIGAHHKE
                     SIVVCDTTEAAINALNLLLQNEDERLAIGKAGQQFVQENYSWKKAAKNLI"
     gene            complement(2748..>3336)
                     /locus_tag="PKN22_07810"
     CDS             complement(2748..>3336)
                     /locus_tag="PKN22_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNS42649.1"
                     /translation="IEPFERNNVDPWYAQAILLIESPGKLAKSISGAYGAFQLMAGVA
                     RAQGIRVDKYVDERKDFDRSAYAASNLIKRVCIPETKKILDKYQLHYNETDIWFRLLV
                     LHVYHAGALNVAAAVDKINPTTGGQSIITRLWQTKAASFGNNSQNYTQLALASQLILD
                     DLIHNNCDYIHSCAEDVELAGNTSPNPSKNKLLID"
BASE COUNT         1077 a          641 c          611 g         1007 t
ORIGIN      
        1 tgaactcatc caattattga aaaaaaatgg gtatgaacct gttttattgg gtggcgagca
       61 agaacatgaa aaaaacagca tgcttgcaca aacaacaggc gcaacctatt tgggctattt
      121 taatttgcaa caattcatca atttgatgta tcaaatggat ttgattgtaa cgcaagtaac
      181 catggcgatg cacattacca ttgctttgca aaagaaaatt gtgttgatga acaacatttt
      241 taaccctcac gaatttgaac tttatggtcg tggtgtgttg gttgaacctt ccaaagaatg
      301 catttgttat ttcgatggaa cttgtaaaaa tggcaattcg tgcatgaaag atttggcgcc
      361 ccaaactatt tttgaagaaa ttaaaaaaca gttgtaaatg aacgttttac aacttacata
      421 ccgcgtacct tatcctgcaa cagacggcgg tgctattggc atttacacca tcaccaaagg
      481 gcttaatgac aacggctgca cggttgattt agttgccatt aacactccaa aacacagtca
      541 gcctgcagat gcaatgcaac accttgttcg caatcaaatt gacgtttttg ttgatacttc
      601 tatttcgcct tttaagttgt tgaccaacct cttgtttcgt tccattccct acaacattga
      661 acgttttgtt tcaaaggaag tggaaaaaca attggaaacc attttaacca acaacaccta
      721 cgattttatt catatcgatg gtgcttttgt ggcttattac atagataaaa ttcgtcaatt
      781 ctctcaagcg cccattattg ttcgtccaca caatattgaa tacgttattt gggagcgact
      841 tgccatcaat gaaaaaaatc cacttaaaaa atggtttttt gcgcatcttg ccaagcgttt
      901 aaagcgtttt gaagccgatt attacaacaa agcagatgga attgcagcca ttacgcctga
      961 agataaaaat cgattattga atctaaatgt taaaaaacca attgacgttg ttccagtagg
     1021 cgtgcttttt aagtccaatt cagttcaaca atcaattcag caagaacaag atactatctt
     1081 ctctatcagt gcattggatt ggatgccaaa tattgaagga ttaaattggt ttttggaaaa
     1141 tgtatggcca ctgttgttgc aaaaaaagcc gacagttcag ttgcatattg ctggaaaatc
     1201 aaccccagat tggctgaaaa acgcaactta tcctaacaca actgtccatg gatttgttga
     1261 cgatgctcag gcgtttaaac agcactacca attaatgttg gttccacttt tatcgggtgg
     1321 cggaatgcga gtgaaaatca ttgaaggatt ggcagaaggc aaatgcatca tcagtacaac
     1381 cattggtgca gaaggtgttg cctataccga taaagaaaac attgtaattg ccgacacgcc
     1441 aacacaatgg gtagatgcca tttgttttta ccttgaaaac gaagaagccc gcaaaaacat
     1501 agagcaaaaa gctgcacaat tggcacacca agtatatgaa aatacaatag taaccaaagg
     1561 ttatgttgat ttgtataata aattaacttc tttgaaatga ataatttacc caaattagtt
     1621 gtagtgcttt ccagatttcc ttttccatta gaaaaaggcg ataaattgcg ctcgtactat
     1681 caaatcaaag agttagcaca acactatgaa attcatttaa ttgccttgag cgatgtagat
     1741 gttgcaatta gcagcaaaaa tgaattggcc aactattgca aaacaattac agtttatcgt
     1801 ttgtcaaaaa tttcggttgc tttcaattca ttggtagcgt tattgggcaa aaaacctgtg
     1861 caagttggct atttttacaa ttattttgta gcacgaaaaa ttcaacgtca gttacaacat
     1921 ttgcaacccg atcatttgtt ttcgcaatta attcgcacag ctgaatatac aaaaaattac
     1981 atgggttgcc ccaaaacact ggactacatg gatgcctttt caaaaggttt tgaacgaaga
     2041 gcagaaaaat ctccttggta ttccaaatgg ttttttcaac ttgaatacaa acgtttgcaa
     2101 gtttacgaac gcttgatgtt tgattatttt gataaaaaaa ccattatttc agagcaagac
     2161 aaacgctata tttttcatcc caatcgttct gaaattgtat gcgtacccaa cggaattgat
     2221 gcatcttttt ttgaatcgcg ttcactttca aaaaaatacg attttgtttt tgtaggcaac
     2281 atgaattatg cccccaacgt ggaggctgtt ttatacattg ccaaagaaat tcttccttcc
     2341 ttccctatgt cacaattttt aattgctggt gccaatccac atccacgtgt aaagcgatta
     2401 gcagaacaaa attcaaacat tgcggtttct ggttggatgg acgatatacg cgatgcttat
     2461 gcaagtgcaa aaatatttct cgctccgatg aaaactggca caggcatgca aaacaaatta
     2521 ttggaagcca tggcgatggg gattccgtgc atcacttctt cgttggccaa taacgccatt
     2581 ggtgctcatc acaaagaatc tatcgttgtt tgcgacacta cagaagcagc catcaatgca
     2641 ttgaatttat tgctacaaaa cgaagacgaa cgtttggcaa taggaaaagc aggacaacaa
     2701 tttgtgcaag aaaattacag ttggaaaaaa gctgctaaaa atctaattta gtcaattaat
     2761 aacttatttt ttgatggatt gggagaggta ttcccagcca attccacatc ttctgcacac
     2821 gagtggatgt aatcgcaatt gttgtgaatt aaatcatcca aaatcaactg tgaagccagt
     2881 gctaattgcg tataattttg agaattatta ccaaaacttg ccgcttttgt ttgccacaaa
     2941 cgcgtgatga tggattgtcc gcctgtggtt ggattaattt tatcaacagc tgcggcaaca
     3001 ttcaacgctc cagcatggta cacgtgtaaa accaacaaac ggaaccaaat atcggtttca
     3061 ttgtagtgaa gctggtattt atccaatatt tttttggttt ctggtatgca aactcgctta
     3121 attaaattag atgcagcata agccgaacga tcaaaatctt tgcgttcatc cacgtattta
     3181 tccacacgta ttccttgtgc acgcgccaca cctgccatca attgaaaagc accataagct
     3241 ccagagattg attttgccaa tttgccaggt gattcaatca ataaaatagc ttgtgcatac
     3301 caaggatcaa cattatttct ttcaaatggt tcgatt
//
LOCUS       DAOQVQ010000252         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32771_length_3333_cov_3.276998, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000252 DAOQVQ010000000
VERSION     DAOQVQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32771_length_3333_cov_3.276998"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKN22_07815"
     CDS             complement(<1..620)
                     /locus_tag="PKN22_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNS42650.1"
                     /translation="MKFVLVEFFKDIHPVLAALIATLFTWFLTALGAALVFFFKKENR
                     GLLDAMLGFTGGVMIAASFWSLLSPAIEMSEKTYPDSLKWMPAAVGFLMGALFLFILD
                     KKMPHLHINFDTKEKEGIKTDLHNTTLLILAITLHNIPEGLAVGVLFGATAVGGEELL
                     IPAITLAIGIGIQNFPEGFAVAMPLRRAGVSRAKSFWYGQLSAVVEP"
     gene            complement(986..1777)
                     /locus_tag="PKN22_07820"
     CDS             complement(986..1777)
                     /locus_tag="PKN22_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS42651.1"
                     /translation="MKAEVEDLEKAQSHYLLAKIGKRVLRPGGVEMTKQLIERLNITE
                     SDTLVEFAPGLGYTASLVVKKLPSLYIGIEADSDHVLLLRKKVRAINGTRVEFVQGDA
                     ENTGLNADSCDKLFGEAVLSMHANQRKSRIIKEASRILKKGGLYAIHELELNLKGEEI
                     SKEAQIQRELALVSNVNARPLTISEWTELLKSEGFVIRNIERRPLRVLEPQRILEDEG
                     LFNTLRIVFNVLRLPKARKRILEMRRTFKANDAYLNAVSIIAEKL"
     gene            complement(1840..2850)
                     /locus_tag="PKN22_07825"
     CDS             complement(1840..2850)
                     /locus_tag="PKN22_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS42652.1"
                     /translation="MIETDIIIIGAGPVGLFTVFEAGLLKLKCHLIDSLPQPGGQCSE
                     IYPKKPIYDIPGFPSILAGDLTTNLMEQAAPFKPGFTLGEAAVSIDKTDDDKFIVTTV
                     KGTQHIAPVVMIAGGLGVFEPRKPPIDNIVDFEDKGVEYIIKDPEFYRGKRCVIAGGG
                     DSALDWSIFLADKNIASEVVLVHRSASFRGHLDSVQKVINLAEAGKIKLVTEAEVVGL
                     NGEGQLQNVVIKHNSGEEFIKETDHFIPLFGLKPSLGPIANWGLEIEGGAIKVNTEDY
                     STNIPGIFAIGDINTYKNKLKLILCGFHEGTLAVQSAFARIHPDKKNILKYTTVNGVH
                     GF"
     gene            complement(2930..>3333)
                     /locus_tag="PKN22_07830"
     CDS             complement(2930..>3333)
                     /locus_tag="PKN22_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS42653.1"
                     /translation="NPTSQSTTATVSETTNYTVFITDGVCTKSDTVWVKAYPFVCAEP
                     FVFVPNAFTPNNDGENDILYVYGAMVKGILFRIYDRWGELVFETTDRHVGWNGVFRGK
                     QLDPDVYDYYLEVDCIDGGSNIIKGNITLMR"
BASE COUNT         1080 a          691 c          463 g         1099 t
ORIGIN      
        1 ggctctacaa ctgcagataa ttgtccatac caaaaacttt ttgctctaga aacacctgct
       61 cttctcaatg gcattgcaac tgcaaatcct tctggaaaat tttgaatacc tataccaatt
      121 gctagtgtaa ttgcgggaat taataactcc tcacctccta cagctgttgc tccaaacaat
      181 actccaacag ccaatccttc tggaatattg tgaagggtta ttgctaaaat caataaagtt
      241 gtattatgca aatctgtttt aatcccttct ttttcttttg tatcaaaatt gatgtgcaaa
      301 tggggcattt ttttatctaa aataaacaaa aacaaagctc ccatcaaaaa accaacagca
      361 gctggcatcc attttaatga atctggataa gttttctcag acatttcaat tgcaggactt
      421 aaaagcgacc aaaaacttgc tgcaatcatt actccacctg taaaacccaa catagcatcg
      481 agcaaacctc tattctcctt cttaaaaaag aatactaaag cagcacccaa ggctgttaaa
      541 aaccacgtaa ataatgttgc aattaaagcc gctaatactg gatgaatgtc tttaaaaaac
      601 tctactaaca caaacttgat attttaaaca aaaatataaa aatgtattct caaacaatcg
      661 cttaattaat aattattatt aagtaccttt tttatatcat tggattttta gcattttcca
      721 tcgttgatta catgctttta tgtcaatatc gaacgatttc gatattgcga tttcaacaaa
      781 aaaatactgt attttttttt cgaaatcttc tttgcaatgc catatcgaca tatttcgata
      841 tcaacatttt ttgatataat ttctattgtt aaatttgagt tcacaagaac atattcacat
      901 atcgaaatag ttcgatatca tgatttaaca atcaattaaa taccaattac ttacacctat
      961 atcgaaatat cgaaatagtt cgatattaca atttctctgc aataatcgaa actgcattca
     1021 aatacgcgtc atttgcttta aaagtcctgc gcatttccaa aattcgtttt cttgcttttg
     1081 gcaatcgaag aacattaaat acaatccgca gggtattaaa caaaccttca tcctctaaaa
     1141 ttcgttgagg ctcaagcacg cgcaaaggtc gtcgttctat atttcttatt acaaaacctt
     1201 cacttttcaa aagctctgtc cattcagaga tggtgagcgg acgtgcatta acatttgaaa
     1261 ccaaagccaa ttctcgctgt atttgtgcct cttttgaaat ttcttctcct ttgagattga
     1321 gttccaactc atgaattgcg tagagtccac ctttttttag tatgcgacta gcttctttaa
     1381 ttattcttga ttttcgttgg ttggcatgca tgctcaacac tgcttcacca aaaagtttat
     1441 cacatgaatc tgcatttaga ccagtatttt ctgcatcgcc ttgaacaaat tctacacgtg
     1501 taccattaat tgccctaact ttctttcgta aaagcagaac atgatcacta tctgcttcaa
     1561 ttccaatata taaagaagga agttttttaa caactaatga agcggtgtac cccaaaccag
     1621 gtgcaaattc aactaaagta tccgattctg taatgtttaa tcgctcaatc aattgcttgg
     1681 tcatttctac tccacctgga cgtaaaacgc gttttccaat cttggcaagt aaataatgac
     1741 tttgcgcttt ctctaaatct tctacttcgg ctttcatttt ccattttttc acaaacttac
     1801 tatttagaat aattctaaac aagtgtttct gcgaaaaatt taaaatccgt gaacgccgtt
     1861 aacagttgta tattttaaaa tatttttctt gtcagggtgt atacgtgcaa aagcagattg
     1921 aacagccaat gtaccttcat ggaaaccaca caaaatcaat tttaatttgt ttttataggt
     1981 atttatgtcg ccaattgcaa aaataccagg aatattggtt gaataatctt cggtatttac
     2041 cttaattgct ccaccttcaa tttctaagcc ccaattagca attggaccca aagatggttt
     2101 taagccaaat aatggaataa aatgatcggt ttcttttata aactcttcgc ccgaattgtg
     2161 ttttataaca acattttgca attgtccttc tccattcaaa ccaacaacct ctgcctcagt
     2221 taccaatttg atttttccag cttcggctaa gtttataact ttttgaactg aatctaaatg
     2281 acctctaaac gatgccgaac ggtgtaccaa aacaacttca cttgcaatgt ttttatctgc
     2341 taagaaaata gaccaatcca atgcagagtc acctcctcca gcaattacac atcgttttcc
     2401 tctataaaat tcagggtctt tgatgatgta ttctacacct ttatcttcaa aatctacaat
     2461 gttatcaata ggtggttttc taggttcaaa aacacctaaa ccaccggcta tcattacaac
     2521 aggtgcaata tgttgtgtac ctttaacagt ggtaacaata aatttatcat cgtcagtttt
     2581 atcaatagaa acagctgctt ctcctaaagt aaacccaggt ttaaaaggcg ccgcttgctc
     2641 catcaaattg gttgtcaaat cacctgctaa aatggatgga aatccaggaa tatcataaat
     2701 tggtttttta gggtaaatct ctgaacattg acctccaggc tgtggaagtg agtcaattaa
     2761 atgacatttt aacttcaaaa gtcctgcctc aaaaactgta aaaagtccca ccggaccagc
     2821 tccaataata attatatctg tttctatcat cgcaaaggtt tcatttattc gaccacaaaa
     2881 ttagtcaaaa caaatggttt aaccttgtta aaaagataaa aatatccaac tatcgcatca
     2941 acgtgatgtt tcctttaatg atgttgcttc caccatcaat acaatcaact tctaaataat
     3001 agtcataaac atctgggtcc aattgcttac ctctaaaaac tccgttccag cctacatgtc
     3061 ggtctgttgt ttcaaaaacc aactcgcccc aacggtcata aattctgaac aaaatgcctt
     3121 ttaccattgc accgtataca taaagaatgt cgttttcgcc atcattatta ggtgtaaatg
     3181 cattgggaac aaatacaaat ggttctgcac aaacaaatgg atatgctttc acccaaactg
     3241 tatcactctt cgtgcaaacg ccatctgtaa taaaaacggt ataatttgtt gtttcactaa
     3301 ccgttgctgt tgtagattga gaagttggat ttt
//
LOCUS       DAOQVQ010000253         3330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32800_length_3330_cov_3.330382, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000253 DAOQVQ010000000
VERSION     DAOQVQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32800_length_3330_cov_3.330382"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN22_07835"
     CDS             <1..516
                     /locus_tag="PKN22_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNS42654.1"
                     /translation="LTKVIAGAKWMIEAKKELKSATPHLIFQFLAVAPNEHEIPGVFA
                     LGKEMGIDEVRIKTAQLYDYKNGNKLMPKNEKYARYKLQSDGTYALKWKTGNHCWRMW
                     SSCVLTWDARVVPCCFDKDALHQLGDLTQQTFDEIWHSKRYGGFRNAILRNRNTIDIC
                     KNCSEGAKVWA"
     gene            complement(513..1130)
                     /locus_tag="PKN22_07840"
     CDS             complement(513..1130)
                     /locus_tag="PKN22_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNS42655.1"
                     /translation="MKTKLLLLLGLVVLSFGCKKGQANITVEGNVYDATFSKALANAQ
                     IVVTTSSKGESKHILTTQTDSEGNYTFDVKRERFETLNITISKENYFDIQSSVLFDDL
                     SVKNKNVHNFSSTGKSWAKIHLKHTGDNTTKLDIVKSIGKSGCEECCSSDYQQFVGNL
                     DTSYYCINDANTNFEVTYFKQFSSTNGKKNAITPFMDTVEILIEY"
     gene            complement(1140..1412)
                     /locus_tag="PKN22_07845"
     CDS             complement(1140..1412)
                     /locus_tag="PKN22_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNS42656.1"
                     /translation="MIKFVRNMAIAEGISYLAFALTMPLKYIWGIKEPNFIVGSAHGF
                     LFIAFCLLVVVAALKFKWDAKKTGILLLSSLIPFGTFWSEKKYLRH"
     gene            1546..1980
                     /locus_tag="PKN22_07850"
     CDS             1546..1980
                     /locus_tag="PKN22_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNS42657.1"
                     /translation="MKKLFYLVLSVLLFIACDEIPSKESQEKYDVSKGIGKFNEQNVD
                     ISTFDLKRAENGKLISETKCASCHKFSNERLVGPGWKGVTKRRKHYWLMNFITNPDAM
                     IDNDPILAQELEICLTRMPNQNLSDDEARDILEFMRQIDGAH"
     gene            2066..3259
                     /locus_tag="PKN22_07855"
     CDS             2066..3259
                     /locus_tag="PKN22_07855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNS42658.1"
                     /translation="MLEFSDRLNNMEESATLAMSRISRELRAQGKDIISLSLGEPDFD
                     TPQFIKDAALEAMNNNFTRYTPVNGYEDLRESISKKFKRDNNLNYSKNQIVVSTGAKQ
                     SIANVVLSLINEGDEVIVPTPYWVSYIEIVKLAGGVPIEVETGIETDFKITPAQLESA
                     ITSKTKMMIFSSPCNPSGTVYSKEELEQLAQVIAKYPQLVVVSDEIYEHINFVGKHAS
                     LAQFESIYNQVVTVNGASKAWAMTGWRIGFIGAPQNIADACTKMQGQFTSGTCSIAQK
                     ATIAAMEANPTVLKDMVAAYKGRRDLVLEKLKAIDGIKVNVPEGAFYVFPDVSSYFGK
                     SYNGKTIHTAEELCMFILNEALVALVTGEAFGDKKCIRISYATSEEVLIEALDRIKMA
                     LQKLK"
BASE COUNT         1173 a          531 c          599 g         1027 t
ORIGIN      
        1 ttaaccaaag ttattgcggg tgcaaaatgg atgattgaag caaaaaaaga gttgaaatca
       61 gcaactcccc atttaatatt tcaatttttg gcagtggcac ccaacgagca cgaaatacca
      121 ggtgtttttg cacttggaaa agaaatggga attgatgaag tgcgcatcaa aacagcacaa
      181 ctctacgatt ataaaaacgg taacaagtta atgcctaaaa atgaaaaata tgcccgctat
      241 aaattacaat ccgatggcac ttatgcgctc aaatggaaaa cgggtaatca ctgctggaga
      301 atgtggtcga gttgcgtact aacgtgggat gcaagagttg ttccctgttg ttttgacaaa
      361 gatgctttgc accaattagg cgatctaact caacaaacct ttgatgaaat atggcattcc
      421 aaacgatatg gaggttttag aaatgcgatt cttagaaaca gaaatacgat tgatatttgt
      481 aaaaattgct cagaaggagc aaaagtttgg gcttaatatt caattaaaat ttcaaccgta
      541 tccatgaatg gagtaatggc atttttcttt ccattggttg atgaaaattg cttgaaatag
      601 gtcacctcaa aattggtgtt ggcatcgttg atgcaataat aagaggtgtc taaattccct
      661 acaaattgtt ggtaatcact agaacaacac tcttcacatc ccgattttcc aatcgattta
      721 acaatatcca attttgttgt gttgtcgcct gtatgtttta aatgaatttt tgcccacgat
      781 tttcctgttg acgaaaaatt atgcacattt ttattcttaa cactcaaatc atcaaacaaa
      841 accgatgatt gaatgtcaaa ataattctct ttgctgatgg tgatgttcag cgtttcaaaa
      901 cgttctcttt ttacgtcaaa tgtgtaattg ccctcactgt ctgtttgagt tgtcaaaatg
      961 tgtttgcttt ctcctttaga agatgttgta acaacaattt gagcatttgc caaggcctta
     1021 gaaaaagtag catcataaac atttccctca acggtaatgt tggcttgacc tttcttacaa
     1081 ccaaaagaca aaacaacaag tccaagtagt aacagcaatt ttgttttcat acatgcaaat
     1141 tagtgacgta aatatttttt ttctgaccaa aaagtaccaa atggtatcaa cgaagaaagt
     1201 aataaaatgc ctgttttttt agcatcccat ttgaatttta atgcagccac aacaaccaac
     1261 aagcaaaaag ctataaataa aaatccgtga gcgcttccaa caataaagtt aggttctttt
     1321 attccccaaa tgtattttaa aggcattgtt aatgcaaatg ctaaatagga aattccttcg
     1381 gcaatagcca tgtttcttac aaatttgatc atttcttttt tttaggcaaa tatacgaaaa
     1441 caaccaacat aaaacagctt aaaaactacc tgattgttgt gattgtaatc atattttatt
     1501 tgcaatcaat attttatatt tgtaaatcgt taaaacaata aacttatgaa aaaactattt
     1561 tatcttgttc tatctgtttt gctttttata gcatgcgatg aaattcctag taaagaaagt
     1621 caagaaaaat acgatgtatc taaaggaata ggtaagttca atgaacaaaa tgtagatatt
     1681 tcaacatttg atttgaaacg tgcagaaaat ggaaaactga taagtgaaac caaatgtgca
     1741 tcttgccata agttttcaaa tgaacgattg gtcggaccag gatggaaagg agttaccaaa
     1801 cgcagaaaac attattggtt aatgaatttt ataaccaatc ctgatgcaat gattgacaac
     1861 gatcctattc ttgcccaaga attagaaatt tgtttaacac gaatgcctaa tcaaaattta
     1921 agcgacgatg aagcacgtga tatattagaa tttatgcgtc aaattgatgg tgctcattag
     1981 tttctaagaa taattggtct aaaaaattaa taaatagtta ttttatctca gttctttgtt
     2041 tagttttgtg taaaaacaaa ataatatgtt ggaattttcc gatcgattaa acaatatgga
     2101 agaatctgct acactagcga tgtctagaat tagtagagaa ttaagagcgc aaggaaaaga
     2161 tatcatttct ttgagtttag gggaacccga tttcgacacg cctcaattta ttaaagatgc
     2221 ggcgctcgaa gcaatgaaca ataattttac tcgatataca cctgttaacg gttacgaaga
     2281 cttacgcgaa agcatttcta aaaaattcaa acgcgacaat aatttgaatt attcaaaaaa
     2341 ccaaattgtt gtttcaacag gtgcaaaaca atccattgcc aatgttgtat tgagtttaat
     2401 taatgaagga gatgaagtta ttgtgccaac accttattgg gtttcttaca ttgaaattgt
     2461 aaaacttgca ggaggtgttc ctattgaagt agaaacagga atcgaaaccg attttaaaat
     2521 tacaccggca caattagaaa gtgcaattac atccaaaacc aaaatgatga ttttttcatc
     2581 gccttgcaat ccatctggaa cggtttatag caaggaagaa ttggaacaat tagcacaagt
     2641 aattgcaaaa taccctcaat tagttgtggt ttctgatgaa atttatgaac atattaattt
     2701 tgttggaaaa cacgcaagct tggcccagtt tgaatccatc tataaccaag tggttacagt
     2761 aaacggagct tctaaagcgt gggcaatgac tggttggaga attggtttta taggtgcacc
     2821 tcaaaatatt gcagatgctt gtacaaaaat gcaaggtcag tttacatctg gaacatgttc
     2881 aattgcacaa aaagcaacaa ttgcggcaat ggaagcaaat ccaacagtgt taaaagacat
     2941 ggttgcagcg tataaaggac gtagagattt agtactcgaa aaattaaaag ccattgacgg
     3001 aattaaggtc aatgtgccag aaggagcttt ctatgtattt ccagatgttt cctcctattt
     3061 tggaaaatcg tataacggaa aaacaattca tacagcagag gaattgtgta tgtttattct
     3121 caacgaagca ttggtagcac ttgttactgg tgaggcattt ggagataaaa aatgcattcg
     3181 tatttcttat gctacatcag aagaagtttt aattgaagcc ttggatagaa taaaaatggc
     3241 attgcaaaag ttaaaataat ggaattagat cttctatttc aaaagtttaa tcaacttaaa
     3301 gtagcagttg ttggcgatgt aatgattgac
//
LOCUS       DAOQVQ010000254         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32813_length_3329_cov_3.968235, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000254 DAOQVQ010000000
VERSION     DAOQVQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32813_length_3329_cov_3.968235"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /gene="lnt"
                     /locus_tag="PKN22_07860"
     CDS             <1..1473
                     /gene="lnt"
                     /locus_tag="PKN22_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNS42659.1"
                     /translation="ASPLLFIAITPLFIGLHEIIKSEKKNAFIVLFYIYLFKLGWVFT
                     QMFWLKGVTFDTFYLAVLTHSLIFVIILTPILYFLKSAKKDFAFYYILLAWLLFEYLM
                     QNLPLLSPFYILGTAFGDVPLLIKSYSIIGIEGGSLWILLTNFFLFKLLINRKNYVKQ
                     LVALVVVLITPLFLGLLFNSSHSSSKQIKVAALHTNFNPLNYNYALRPTEIIDSLWNL
                     SKNVSKETSALVWPETVITNLGWAHELNFNPNVDSLQQKLVPYPNLSLIFGANLYALG
                     KDTTDKQLNYDPVNNYYYYVYNMSFILTSQKAIEYRSKEIFVPFQEQIPYVNQFPFLK
                     KLITVVGNPNYYAENKNDFNVLRLQNGVSYSPLLCYEICYPHFTSKMSEDVGFIALSG
                     NEHWNTSPIGSRIYFNILKSIAAQNACAIVKSSNNGISAIVDKNGVAIQEKKFNDTGL
                     LEAKIDVKENSNFYSFVSGYTSFLALILAPFLFILARRKK"
     gene            1560..2174
                     /locus_tag="PKN22_07865"
     CDS             1560..2174
                     /locus_tag="PKN22_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNS42660.1"
                     /translation="MHIAIAGNIGSGKTTLTQMLAKHYGWDTHFEDVEQNPYLNDFYE
                     DMQRWSFNLQIYYLNSRFTQIQEIKQSNTSVVQDRTIYEDAFIFAPNLHSMGLMTTRD
                     FENYFSLFNLMDSFVSAPDLLIYLRASVPTLVSHIQQRGRDYEESIRLDYLKRLNERY
                     EAWISTYDKGKLLIVDIDNNNFPENQEDLGKIINSIDAEIHGLF"
     gene            2186..3151
                     /gene="rfaD"
                     /locus_tag="PKN22_07870"
     CDS             2186..3151
                     /gene="rfaD"
                     /locus_tag="PKN22_07870"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNS42661.1"
                     /translation="MIVVTGAAGFIGSCLVSYLNQEGYPDIVVVDDFSKTEKAPNLVG
                     KTIAAKVSRNDFNAWFKDFASEIDFVFHIGARTDTTEFNKAIFDELNERFSQEVWKTC
                     VEHQIPLVYASSAATYGLGEFGYKDDHAIIEKLQPLNPYGDSKNNFDKWVLKQTAFPP
                     FWAGLKFFNVYGPNEYHKGRMASVIFHAFHQIMKTGEMKLFRSHNPNYSDGGQLRDFV
                     YVKDVISVCKFLMENKPQSGIYNLGSGKARTFLDLAKNTFKALNKEEHISFIDTPADI
                     RDKYQYFTEADMSKLIAQGYTKPFYTLEEGVADYVENYLSAEKYY"
     gene            3188..>3329
                     /locus_tag="PKN22_07875"
     CDS             3188..>3329
                     /locus_tag="PKN22_07875"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685894.1"
                     /note="catalyzes the formation of 2-amino-3-oxobutanoate
                     from acetyl-CoA and glycine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNS42662.1"
                     /translation="MYGKMKDFLASELKAIEEGGIYKRERIITSPQGANVQVSTGDNV
                     VIM"
BASE COUNT         1098 a          541 c          565 g         1125 t
ORIGIN      
        1 gcatctcccc tgctattcat tgcaataact ccattattta ttggtcttca tgaaataata
       61 aaatcagaaa aaaagaatgc ctttattgtt ttattctata tttatttatt caagttagga
      121 tgggttttta cgcaaatgtt ttggctcaaa ggcgtcactt ttgacacttt ttatttagcg
      181 gtattaactc attcacttat ttttgttatt attttaactc ctatacttta cttcctcaaa
      241 tccgctaaaa aagattttgc gttttactat atcctactgg cttggttgct atttgaatat
      301 ttaatgcaga acttgccttt gttaagtcca ttttatatac ttggaacagc ttttggcgat
      361 gtacctctac taataaaaag ctactcaata attggaattg aaggaggaag tttgtggata
      421 ttgttgacca atttttttct tttcaagctt ctaattaaca gaaaaaacta tgtaaaacaa
      481 ctagtcgcct tagtagttgt gcttataact cctctatttt taggtttgct tttcaattct
      541 tctcattctt cttctaagca aataaaagtg gctgctttgc acactaattt caatccgttg
      601 aattacaatt atgcattacg gccaacagaa atcatcgatt cgttgtggaa tttatccaaa
      661 aacgtatcca aagaaacatc agctttggta tggccagaaa cagtgattac taatttagga
      721 tgggcacatg aattaaattt taaccccaac gttgattcac ttcaacaaaa attagtgcca
      781 tatccaaatt tatcattgat ttttggtgcc aatttatatg ctttaggaaa agatactact
      841 gacaaacaat tgaattatga tcctgtcaac aactattatt attatgtata taatatgtca
      901 ttcattctta cttcgcaaaa agccattgaa tacagaagta aagaaatttt tgttccattt
      961 caagaacaaa ttccctatgt caatcaattt ccattcttaa aaaaattgat taccgtggtt
     1021 ggaaatccta attattatgc ggaaaataaa aatgatttca atgtgttacg cttgcaaaat
     1081 ggagtttctt attcgcccct attgtgttat gaaatttgct atcctcattt tacctcaaaa
     1141 atgagtgagg atgtaggttt tattgcactt tcgggaaatg agcattggaa cacaagccca
     1201 attggctcca gaatttattt caatatactt aaatccatag ctgcccaaaa tgcttgtgca
     1261 attgttaaaa gctccaacaa cggaatttcg gcaattgttg acaaaaacgg tgttgcaatt
     1321 caagaaaaaa aattcaacga cacaggctta ttggaagcaa aaattgatgt gaaagaaaac
     1381 tctaactttt attcatttgt tagtggttac acttcatttt tagcccttat attagcaccg
     1441 tttttattca ttttagcacg tcgaaaaaaa tgaattagtg gatttaaaaa agatttgaca
     1501 aatttcaatt tgctaatttg ttcaattatt gtaaatttaa cctctcaaat taattgatta
     1561 tgcacattgc aatcgctgga aatattggat ctggaaaaac aacgttaaca caaatgttgg
     1621 caaaacatta tggttgggat actcattttg aagatgttga acaaaatcct tacttaaatg
     1681 atttttatga agacatgcaa cgttggtcgt tcaaccttca aatttattac ttgaattcgc
     1741 gttttacaca aattcaagaa attaaacaat caaataccag tgtggttcaa gacagaacaa
     1801 tttatgaaga tgcgttcatt tttgcgccta acctccattc aatggggtta atgacaacgc
     1861 gtgattttga aaactatttt tcattgttca atttgatgga ttcatttgtt tctgctcccg
     1921 atttattgat ttatttgcgt gcaagtgtgc ctacattggt gagccatatt caacaacgcg
     1981 gtcgcgatta cgaagaaagc atccgtttgg attatttgaa acgcttaaac gagcgctacg
     2041 aagcttggat ttctacttat gacaaaggaa aacttttaat tgttgacatt gataacaata
     2101 acttcccaga aaatcaagaa gatttaggga aaattatcaa ttcaatagat gcagaaattc
     2161 acggtttatt ttaatacaac aataaatgat agtagttaca ggagcagcgg gttttattgg
     2221 aagttgcttg gttagttatc ttaatcagga ggggtatcct gatatcgtag tggtggatga
     2281 tttttctaaa accgaaaaag ccccaaattt ggtgggtaaa actattgccg ctaaagtatc
     2341 tcgcaatgat ttcaatgctt ggttcaaaga ttttgcttct gaaatagatt ttgtttttca
     2401 catcggtgca cgaacagaca caacagagtt taacaaagct atttttgatg aattgaacga
     2461 acgtttttcg caagaagttt ggaaaacatg tgttgaacat caaattcctc ttgtttatgc
     2521 tagttctgct gccacttatg gtttaggaga atttggctac aaggacgatc atgctatcat
     2581 tgaaaagttg caaccattaa atccttatgg tgattctaaa aataattttg ataagtgggt
     2641 gttgaaacaa actgcttttc ctccgttttg ggctggattg aagtttttta atgtttacgg
     2701 acccaacgaa taccacaaag gcagaatggc tagcgttatt tttcatgctt tccatcaaat
     2761 catgaaaacg ggagagatga aattgttccg ttcacacaat ccaaactata gtgatggtgg
     2821 acaattgcgt gattttgtat atgttaaaga cgtaattagt gtttgtaaat tcttaatgga
     2881 aaataaaccg caaagcggaa tttataactt aggttctgga aaagcacgca catttttaga
     2941 tttagcaaaa aacacattca aagcactcaa caaagaagaa cacatttcat ttattgacac
     3001 gcctgccgat attcgtgata aataccaata tttcacagag gccgacatgt ctaaattgat
     3061 tgcgcaaggt tacacaaaac cgttttatac attagaagaa ggtgttgcag attatgtaga
     3121 aaactacctt tctgcagaaa aatattatta ataattattc ggatttactc cacaaaaaca
     3181 aagaattatg tacggtaaaa tgaaagactt tttagcatct gaattgaaag ctattgaaga
     3241 aggaggaatt tacaaacgcg agcgtattat tactagtcca caaggtgcta atgtgcaagt
     3301 gagtactggc gacaacgtag taattatgt
//
LOCUS       DAOQVQ010000255         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_32833_length_3328_cov_4.230370, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000255 DAOQVQ010000000
VERSION     DAOQVQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32833_length_3328_cov_4.230370"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKN22_07880"
     CDS             <1..328
                     /locus_tag="PKN22_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42663.1"
                     /translation="PLQNEKINTIEYTYTVPASYLLHIKNVFCLNQNDIFRYRVYWIY
                     RTGLSNWSNWSQHQFGCYEAVNLTKGYNDYLIYEYEVERNGVLNTYLDTFYINPDYVD
                     TLKIYY"
     gene            775..>3328
                     /locus_tag="PKN22_07885"
     CDS             775..>3328
                     /locus_tag="PKN22_07885"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42664.1"
                     /translation="MRFTLLFTLIFLLFNLHDVNAQCVAKAGLNDTICAGGSVTLGGT
                     STGNGTITYAWTPSAGLSCDDCASPIATPSSTTTYHLTITDSDGCSSSDDVTVVVNPL
                     PNSGFTFSNNNVCANLPIVFTANSIVPGYSYSWNFGNPASGGNNTASTATTIHEFVSY
                     GTGSTTYTVTLTVTTPQGCQTSTTQTVTINNLPQATLMDPISSFKNCDGTNFNMTIYD
                     ATPNTGTNYQIIWGDGTSDYNSTTAPSGESHTYTISDIFDLYYIVTGTNGCIDTAQYI
                     VSNITNPAIGAANPGATTGCGPLTLCFPLSSYSANHHSTIYIVDYGDNTPVDTFTHAA
                     LPPQICHTYTESSCGKPGNAFTFEIQAQNQCDISTTTVTPIRIYTGPQPEFANPPVAC
                     VNTPVSFTNNTIPGYNTTCATTTIYTWDFGDGTANLITPIKTNPTHTYTSPGTYTITL
                     SSQNSCGTFSIAHTICIEAIPNPLYDITPLTSCVPFNVVVTNNSDTADMCEFTFNWQL
                     LNFSSSCQSAQNFSFDNGTTATTWHPQFLYESAGEYTMRLSITNSCGTFNKDTLVKGL
                     DIPRIALAPLDSICLGNSVTPNLTLNDCYDPINTYSWTFNSGTPTASSDENPGPISYT
                     MSGTFPITVEVTNICGSVDSTKTITVFDPPVADAGPDVSYCSGGSAVLGGATAVTGVS
                     YQWSPTTNLTSTNQPTATANPTNTGDTPVIIQYVLTASTASTCLDRDTVNVTVNPLPH
                     LTVNNPTICLGDNTQLIVSATTTPMNYLWNTTADLSCLPCDNPTLSPSVTTTYTVVAT
                     NSFGCIDSISSTVTVNPLPVVNAGNDSTLCDQPIPFNLTGTPAGGSWSGSVNV"
BASE COUNT         1063 a          642 c          547 g         1076 t
ORIGIN      
        1 tcctttacaa aatgaaaaga tcaacacaat agaatatact tataccgttc cagcatcgta
       61 tttgttacat attaaaaatg tattttgtct taatcaaaac gatatattta gatatcgagt
      121 ttactggata tatagaactg gattatctaa ctggtcaaat tggtcacaac atcaatttgg
      181 ttgttatgag gctgttaatc tgacaaaagg atataatgac tatctaatct acgaatacga
      241 agtggaacgt aatggtgtat taaatacgta tctagataca ttttatataa atccagacta
      301 tgtagataca ctaaaaatat attactaatg aaaccacaga aacacataaa gttattgttc
      361 gttagttcta ataaatttga taagatttga gagataacaa agacctcatc cctacgagat
      421 ttttattggt aaggttcttg ttatttctac acaagtcctg ggactgaaaa gcgtttaatc
      481 atagtaaacc agcatcaagc actgtaggtg cgatagcttt gtagaacaat tagtacccat
      541 ttacatcaag ctccatcgga gcgataccgt tgtagaaaat tgttcgttcg tttatgacta
      601 gctattgctg tttaaaaaat ttgaaaatag atcagactaa cactctcttg tcgaattaag
      661 tagaggtatt tatataatat ctacgataaa tgacgtagat tgcttttttt ttcgtaaaaa
      721 aatatttttt ttccatatct tcgcaaccta attcgcttgg aacattttgt ctttatgaga
      781 tttacattat tatttacgct aatctttttg ttgtttaatc ttcatgatgt aaatgctcag
      841 tgtgttgcaa aagcaggatt aaatgacact atttgtgcag gtggttctgt tacattggga
      901 ggcacatcaa caggaaatgg gacaataact tatgcttgga caccctctgc tggattaagt
      961 tgtgacgatt gtgcttcacc tattgccacg ccatcttcaa caactactta tcatttaaca
     1021 attacagata gtgatggttg ttcttcgtct gatgatgtta cagttgtagt gaatccttta
     1081 ccaaattctg gttttacatt ttcgaataac aatgtgtgtg caaatcttcc tatagttttt
     1141 acggcaaatt caattgttcc aggatattct tattcttgga attttggaaa tcctgcttct
     1201 ggtggaaata atacagcatc cacagcaaca actatccacg aatttgtgtc gtatggaaca
     1261 ggaagcacta cttatacggt gacattaacc gttaccacac cccaaggttg ccaaacatca
     1321 accactcaaa cggttacaat caataattta ccacaggcta cactgatgga tcctatttca
     1381 agttttaaaa actgcgatgg aactaatttt aatatgacta tttatgatgc tactccaaac
     1441 acaggtacaa actatcaaat aatttgggga gatggcactt ctgattataa tagtacaaca
     1501 gctccatcgg gtgaaagcca tacctataca atttctgata ttttcgactt atactatatt
     1561 gtaactggaa cgaatggatg tatcgataca gctcaataca ttgtttcaaa tattacaaac
     1621 cctgctattg gtgctgccaa tcctggggca accactggat gtggtccact aactttatgt
     1681 tttcctttat ccagttatag tgccaatcac cattcaacaa tctacattgt agattatgga
     1741 gataacacac ctgttgatac atttactcat gctgctttac ctccacaaat ttgccatact
     1801 tatacggagt cctcttgtgg taaaccagga aatgctttta cttttgaaat tcaagctcaa
     1861 aatcaatgtg atatttcaac aactactgtc acaccaattc gtatttatac aggaccgcaa
     1921 ccagaatttg caaatccacc tgtagcttgt gtgaatacac ctgtttcgtt cacaaataat
     1981 acaataccag gttataatac aacttgcgca acgactacta tttatacatg ggattttggt
     2041 gatggtacag ctaacttaat tacacctata aaaacaaatc caactcatac ctatacttct
     2101 ccaggaactt ataccataac actaagttct caaaactctt gtggaacttt ttctatagct
     2161 catactatct gtatagaagc tattcctaat cctctatacg atattacacc tttaacaagt
     2221 tgtgtgcctt ttaatgtagt tgtaacaaac aatagtgaca cagctgatat gtgtgaattt
     2281 acattcaatt ggcaattatt gaatttctca tcaagttgtc aatctgcaca aaactttagc
     2341 tttgacaatg gaacaactgc cacaacttgg catccacaat ttttatatga atcggcagga
     2401 gaatatacca tgcgtttatc tattacaaat tcttgcggta cttttaataa agatacgctt
     2461 gtaaaaggtt tggatatccc gagaattgca cttgctccat tggattctat ttgtttagga
     2521 aactctgtaa cgccaaattt aacgttgaat gattgttacg atcctataaa tacgtattca
     2581 tggacattta attcaggaac gccgacagct tcttcagatg aaaatccagg accaatatct
     2641 tatactatgt caggtacttt tccaataaca gttgaagtta ctaacatctg tggaagtgtg
     2701 gattcaacaa aaacaataac tgtatttgac ccaccagttg ccgatgctgg tccagatgtt
     2761 tcctattgtt ctggtggaag tgctgtatta ggtggtgcaa cagcagtaac aggagtttcc
     2821 taccaatggt cgccaacaac aaatttaacg tcaaccaatc aaccaacagc tacagcaaac
     2881 ccaacgaata caggtgatac accggtaatc attcagtatg ttcttacagc ttcaacagca
     2941 agtacttgct tggatagaga tacagttaat gtaacggtta atccgctacc tcatttaacg
     3001 gtaaataatc caactatttg tttgggtgat aacacacaac taatagtttc tgcaacgaca
     3061 acaccaatga attacttgtg gaacactact gctgatttat cgtgtttacc ttgtgataat
     3121 ccaacgcttt cgccctccgt tacaactacc tatacggttg tggctacaaa ttcatttgga
     3181 tgtatagatt ctatttcaag tacagttact gttaacccac taccagttgt caatgcagga
     3241 aatgattcca ctttgtgtga ccaaccgatt ccattcaatt taacaggaac gccagcaggt
     3301 ggttcatggt cgggaagtgt taacgtaa
//
LOCUS       DAOQVQ010000256         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_33438_length_3288_cov_3.560161, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000256 DAOQVQ010000000
VERSION     DAOQVQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33438_length_3288_cov_3.560161"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKN22_07890"
     CDS             <1..453
                     /locus_tag="PKN22_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42665.1"
                     /translation="VPYNKTIFKNIESIKAIDLYTGVDITPKAKEQGKLLIESLIKDI
                     ERFGLEKPEPTEKESFIRVDVNQTNQQTQSTTVSINIEFILEILKGELRNSEIEELKE
                     ILDSSDKPKEKKNRFIEKIKSFGSDVASNILANLLTNPQVYENLGKML"
     gene            521..1045
                     /locus_tag="PKN22_07895"
     CDS             521..1045
                     /locus_tag="PKN22_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HNS42666.1"
                     /translation="MNNWIYKHHASSNTRYVLGEWGINNLICIGINPSTATPENLDPT
                     IRRVKKFAQDNGYDGWFMLNVYPQIETNPKLIDEHINNSLHEENIKEIKQLISEFPTS
                     YIWAAWGNNIQRKPYLFQCLKDVVATINADFQKRWVHLNDLTTKKHPKHPLYLPNNAN
                     FKEFDIHEYLKLHA"
     gene            1102..1908
                     /locus_tag="PKN22_07900"
     CDS             1102..1908
                     /locus_tag="PKN22_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42667.1"
                     /translation="MSLLPIVEIAEFIEKPLTGGTTRPMMIIGNDGKQYVLKIFSKTD
                     AKQRSYTVAEAVANILAKEFDLVVPNGIYTKIDHLTIKKIEQSQPTLFKLLEEKETDK
                     LLFASHFQEGCPIFSPAIKHKSLEISEMETILAFDMLIANDDRRKDKPNILKGKEHYI
                     LIDHEKCFEGLAIHLENMNKGILPHFFKNHLFYTRLNKFAKKSKNNVNFATFEEYFRN
                     LNLEKVCTNVHFLIENGYNEDECNNWLHYLHTQKENCHKFVALLKHKIRE"
     gene            1901..2743
                     /locus_tag="PKN22_07905"
     CDS             1901..2743
                     /locus_tag="PKN22_07905"
                     /inference="COORDINATES: protein motif:HMM:NF022681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNS42668.1"
                     /translation="MNKSFSYSILTYNHSLVLGEVINVGVLLIFPDENVVEFHYPQRL
                     NRIKDLYHNFNESLVKDYLKAFEIKARTLNQYLQDYIFGYHELISNNLLTENASALQF
                     EAVKVGFYTGNYETVAKQYVDLLLGNYKSETVGATNRITTEKLIQQVKSTIYELNPQS
                     KNYLKFDDQRILKNNHVEFKSDFYWQNGAIHHAKAVSFDVNKENNIIDSALLLNAKLR
                     QLEKTSLKNTHIDFIIQPPLQLKFNDAYQEACSILNENEVNKQLYTNSLEYCEIVANQ
                     IKPM"
     gene            2833..>3288
                     /locus_tag="PKN22_07910"
     CDS             2833..>3288
                     /locus_tag="PKN22_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNS42669.1"
                     /translation="MAKGIHTEQTFEEAIVASLIENGGYVQGRSKDFDANLGWFPSYI
                     IQFLKTSQPTLWQKIETIHKADVETKVLQRLAKELDTRGSLDVLRNGFVDYGVKFKMA
                     FFSPETTLNPDAALLYNSNHMSVTRQVYYERNGKNSLDLVLALNGLPIAT"
BASE COUNT         1273 a          505 c          533 g          977 t
ORIGIN      
        1 gtaccgtata ataaaactat ttttaaaaat atagaatcaa taaaggctat agatttatat
       61 acaggagttg atatcacacc aaaagcaaaa gaacaaggaa aattattaat tgagagtttg
      121 attaaagaca ttgaacgttt tggcttggag aagccagagc cgaccgaaaa agaaagtttt
      181 atacgagttg acgtaaatca aactaatcaa caaacccaat caactacagt tagtattaac
      241 atagagttca tcttggaaat tttaaaaggc gaattacgca attctgaaat cgaagagtta
      301 aaagaaattc tggattcatc agataaacca aaagagaaga aaaatcgatt tattgaaaaa
      361 atcaaatcat ttggttctga tgtggcttcg aatattttag caaatctatt gacgaatcct
      421 caagtgtatg aaaatttagg aaaaatgctg taattaaact agtatagaac gctttattac
      481 taaaacgcct ttgagtcatt tttaacaaat tattttgtca atgaataact ggatatacaa
      541 acaccacgca agtagcaaca cccgctatgt tttgggagaa tgggggatca ataatctaat
      601 ttgcattgga atcaacccca gcactgccac accagaaaat ttagacccca ccatacgcag
      661 agtaaaaaag tttgcgcaag acaatggcta tgatggatgg tttatgctga acgtgtatcc
      721 gcaaatagaa accaacccaa aacttattga tgaacacatc aacaattcct tacacgaaga
      781 aaatataaag gaaataaaac aactcatttc agagtttccc acgtcataca tttgggctgc
      841 ttggggaaat aatatacaaa gaaaacccta tttgtttcag tgtttaaaag acgttgtggc
      901 tacaatcaac gcagattttc aaaaacgctg ggtacattta aacgacctaa caactaaaaa
      961 acaccccaaa catcccttgt atttgccaaa taacgctaat tttaaggagt ttgacattca
     1021 tgaatatttg aaattgcatg cataaaagta tttcaaaaaa gatgttctca aacagagaaa
     1081 ggtaaataag atgctaagat tatgagtttg ttaccaatag tagaaatcgc tgaatttata
     1141 gaaaaacctc taactggcgg tacaactcga cctatgatga taataggtaa tgatggcaaa
     1201 cagtatgtat taaaaatttt cagtaaaaca gatgcaaaac aacgtagtta cactgttgca
     1261 gaagctgttg ccaatatttt agcaaaagaa tttgatttgg ttgttcctaa tggaatttat
     1321 acaaaaatag accatttaac gattaaaaaa atagaacaaa gtcaaccaac gctattcaaa
     1381 cttttagagg aaaaagaaac cgataaacta ttatttgcaa gtcattttca agagggatgc
     1441 ccgatttttt ctccagcaat aaagcataag tcgctagaaa taagtgaaat ggagactata
     1501 ttggcatttg acatgttaat tgctaacgac gatagaagaa aagataaacc caatatttta
     1561 aaaggtaaag agcattatat tttaatagac cacgaaaagt gctttgaagg tcttgcaata
     1621 catcttgaaa atatgaacaa aggaatttta cctcattttt ttaaaaatca tttgttttat
     1681 acaaggctta ataaattcgc taaaaaatca aaaaacaatg ttaattttgc aacttttgaa
     1741 gaatattttc gtaatttaaa tttagaaaag gtttgcacca acgtccattt tttaatagaa
     1801 aatggttaca acgaagatga atgcaataat tggcttcatt atcttcatac acaaaaagaa
     1861 aattgtcata aatttgtagc acttttaaaa cacaaaattc gtgaataaat cattttcata
     1921 tagcatttta acttataatc attctcttgt tttaggggaa gttataaatg tgggtgtgct
     1981 tttgattttt cctgatgaaa atgtggttga gtttcattat cctcaaagac taaatcgaat
     2041 taaagattta taccataatt ttaatgaaag tttggtaaaa gattatttaa aagcatttga
     2101 aataaaagca agaactttaa accaatattt acaagattat atatttggtt atcacgagct
     2161 catttcaaat aatttactca ccgaaaatgc atctgcttta cagtttgaag ccgtaaaagt
     2221 tggtttctat acaggtaatt atgaaacagt tgcaaagcaa tatgttgact tattactagg
     2281 taattataaa tcagaaacag ttggtgctac aaatagaatt accacagaaa aattgattca
     2341 acaggttaag tcaacaatat atgaactaaa ccctcaatca aaaaactact taaagtttga
     2401 tgatcagcgt attttaaaaa ataatcacgt tgagtttaaa tcagatttct attggcaaaa
     2461 tggagcgata catcacgcaa aggcggtaag ctttgatgtt aataaagaaa acaatattat
     2521 tgatagtgct ttgttactta atgcaaaatt gagacaacta gaaaaaacct ctcttaaaaa
     2581 cacgcacatt gattttatta tccaaccccc tctacaatta aaattcaatg atgcttatca
     2641 agaagcatgt tcgattttga atgagaatga agttaataaa caattgtata ctaattcatt
     2701 agagtattgt gaaatcgttg ctaatcaaat aaaaccaatg tagttttcac aatatttctt
     2761 atcaaaaatg tcttttctat aaaactgtaa ttatatttac atagaacttg aatttacaaa
     2821 aatcataaag aattggcaaa aggaatacac acagaacaaa cttttgaaga agcaatagtt
     2881 gcaagtttaa tagaaaatgg aggatatgtg caaggtcgtt ccaaagactt tgatgcaaat
     2941 ttgggatggt ttccaagtta cattattcag tttctgaaaa cttcacaacc aacgttgtgg
     3001 cagaaaatag aaaccatcca taaagccgat gtggaaacaa aagtgctgca acgcttagca
     3061 aaagagttgg atacacgcgg ttcattggac gtgttacgca atggttttgt tgattatggt
     3121 gtaaaattta aaatggcgtt ttttagtcca gaaaccacac tcaaccccga tgcggcattg
     3181 ctctataatt ccaatcacat gagcgttaca cgccaggtgt attacgaacg caatggcaaa
     3241 aactcactgg atttggtgct tgctctaaac ggacttccta ttgctacg
//
LOCUS       DAOQVQ010000257         3282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_33526_length_3282_cov_3.621630, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000257 DAOQVQ010000000
VERSION     DAOQVQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33526_length_3282_cov_3.621630"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKN22_07915"
     CDS             complement(<1..267)
                     /locus_tag="PKN22_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42670.1"
                     /translation="MGDYKGVISDFIGVKIMLFLQKSCSYGTKKRLIIGNRHKAPSER
                     YRCRKIFINHIKHRRCDAFVKILIRKNNIEDTSFTLNNKQISIFQ"
     gene            complement(384..2501)
                     /locus_tag="PKN22_07920"
     CDS             complement(384..2501)
                     /locus_tag="PKN22_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNS42671.1"
                     /translation="MNKIVCAFFLVIASFSFGQKELTLKDAVLQQYRSFYPERPVSFN
                     WYSSRQEYSYLKDYTTLMKSSVKAKTDETLITIQDVNSIAGVKFAWFSGFEWLNPSEF
                     LINNGQQYVRVNPAAKTATIFNELQENADNALLSSASLYVAYTIENNVYVSLGKGNAV
                     QVTNFSDKNIVSGQAIARSEFGISGGLFWSPNGKILAFYQKDETDVHTYPLVNINETP
                     AELMPIKYPMTGQKSEKPRVGIYNTISKNTVYITPRAASDDYLTNLTFTPDNNFVVIA
                     EVNRDQNHMQLNVYDATTGAFVKTLLEEKNDKWVEPEHPAFFPSNKSNNFVWISEKDG
                     FNNLYYYDFNGKLIKQLTANKFVAKEILTAKKDVIYFSATGENPLNTLIYSVTLKGKQ
                     KLLSQTEGTHEFALSLDGSLYYDGYSNHSTPFKAGIFSQSGKEVKHLLTASNPLADYK
                     IGKTEIGSISAKDGSKLYTRLIKPSNFDASKKYPVLVYVYGGPHAQLITNSWYDGASL
                     WMNWLAEQGYLVFTVDGRGSAERGFAFESQIHRQLGTVEIEDQLSGVDYLKSLNYVDG
                     NRIAVHGWSFGGFMTTSLLLRHPGVFTCGVAGGPVTDWKYYEIMYGERYMDRPEQNEE
                     GYKQASLLTHAANLKDDLLLIHGTVDDVVVMQHNFALVKKFVDLGIQMDFFPYPMHKH
                     NVTGKDRVHLMTKVLNYVMEHNK"
     gene            complement(2509..>3282)
                     /locus_tag="PKN22_07925"
     CDS             complement(2509..>3282)
                     /locus_tag="PKN22_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNS42672.1"
                     /translation="KTTDGKDMLVWMILPPNFDATKKYPTLLYCQGGPQSEVSQFFSY
                     RWNFMVMASQGYVVVAPNRRGLPGFGQEWNDAISKDWGGQSINDYLSAIDAATELPYV
                     DKDRLGAVGASYGGYSVYMLAGVHQKRFKTFIAHCGLFNLESWYGMTEELFFATWDVG
                     GPYWQQENKEAFLKNSPHRYVQNWDTPIMVIHGGMDFRVPESEGMQAYQVAQLKGLKS
                     RFLYFPTEGHWVTKPQNALVWHREFYKWLAEDLKPELNK"
BASE COUNT          993 a          656 c          517 g         1116 t
ORIGIN      
        1 ttgaaaaatt gaaatctgtt tgttattcaa agtaaatgac gtgtcttcta tgttgttttt
       61 tctgattagt atttttacaa aggcgtcaca cctacggtgt tttatgtggt tgatgaatat
      121 ttttctacaa cggtatcgct ccgatggagc tttatgtcga tttcctatga ttaaacgctt
      181 tttagtccca taggaacatg acttttgtag aaacaacatg atttttacac caataaaatc
      241 cgagatgaca cctttgtaat cacccaaatt tattagcact aaggaagtta aagatgattc
      301 tttagttaaa ttttaaccta ttaattataa atcacttatt gctattttgt tttttgtttt
      361 aacaaataat aggtcaaact atcttactta ttgtgctcca tcacatagtt taaaaccttt
      421 gtcattaaat gaactctgtc cttacctgta acattgtgtt tgtgcatcgg ataggggaag
      481 aaatccattt gaatgcctaa atcaacaaac ttttttacca atgcaaaatt gtgttgcatc
      541 accactacat catcaacagt tccatgaatt agtagcaaat catcttttaa attggctgca
      601 tgggttagta aacttgcttg tttgtaacct tcttcgtttt gctcaggacg gtccatgtaa
      661 cgttcaccat acattatttc ataatacttc caatcggtca ctggtccacc tgctacacca
      721 catgtaaata ctccagggtg tcgtagtaat aaagaagttg tcataaatcc accaaacgac
      781 caaccatgaa cagcaatgcg atttccatca acataattca atgatttcaa ataatccact
      841 cccgataatt ggtcttctat ttcaactgtt cccaattggc ggtgaatttg cgattcaaat
      901 gcaaaaccac gttctgcaga acctctacca tcaactgtaa aaactaaata tccttgttct
      961 gccaaccaat tcatccataa agaagcacca tcgtaccatg aattggtaat taattgtgca
     1021 tgtggtcctc cataaacata caccaaaaca gggtattttt tcgacgcatc aaaattacta
     1081 ggcttaatca agcgtgtgta caattttgaa ccatctttag cgctgattga accaatttct
     1141 gtttttccaa ttttataatc tgccaatggg tttgatgccg tcaataaatg ttttacttct
     1201 ttgcctgatt gtgagaaaat tcctgcttta aatggcgtag aatgattgga ataaccatcg
     1261 taatacaaag aaccatctaa tgacagtgca aattcatgcg ttccttcagt ctgtgatagt
     1321 aatttttgtt ttccttttaa agtaactgag taaataagtg tattcaatgg attttctcca
     1381 gttgctgaaa aataaatcac atctttttta gccgttaaga tttctttagc aacaaattta
     1441 ttggctgtaa gttgtttgat taattttccg ttgaaatcgt aataataaag gttgttaaac
     1501 ccatcttttt ctgaaatcca aacaaaatta tttgatttat tagatgggaa aaatgctgga
     1561 tgttctggtt ctacccattt atcgtttttt tcttccaaca atgtttttac aaacgcacct
     1621 gttgttgcat cataaacatt caactgcatg tgattttgat ctcgattaac ttcagcaatt
     1681 acaacaaaat tattatccgg tgtaaaagtt aaattggtca aataatcatc acttgctgca
     1741 cgtggtgtaa tgtaaaccgt atttttagaa atagtattgt aaattccaac gcgcggtttt
     1801 tctgattttt gtcccgtcat tggatatttt ataggcatca actctgccgg cgtttcattg
     1861 atatttacca acggataggt atgcacatct gtttcgtctt tttggtaaaa tgctagaatt
     1921 tttccattag gcgaccaaaa caaaccgccc gaaataccaa attcactacg tgctattgcc
     1981 tgaccagaaa cgatattttt atcactaaaa ttggttactt gcaccgcatt tcctttgcct
     2041 aatgaaacat acacgttgtt ctcaatggta taggccacat aaagtgatgc tgatgaaagt
     2101 aaagcattat cagcattttc ttgcaattca ttaaaaatag ttgctgtttt tgctgcaggg
     2161 ttaacacgca catattgctg accattattg attaaaaact cacttggatt taaccattca
     2221 aagcccgaaa accatgcaaa ttttactcct gcaatactgt ttacatcttg aatagtaatc
     2281 aatgtttcat ccgtttttgc cttaacgctg gatttcatca atgtggtgta atctttcaaa
     2341 tatgaatact cctggcgcga tgaataccaa ttaaaagaca ctggtcgctc tggataaaac
     2401 gagcgatatt gctgaagaac agcatctttt aaggttaatt ctttttgtcc aaatgaaaaa
     2461 cttgcaatta ctaagaaaaa tgcgcaaact attttattca tactgttttt atttattcaa
     2521 ttctggtttt aaatcttctg ccaaccactt gtaaaattca cggtgccaca ccaatgcatt
     2581 ttgtggtttt gttacccaat gtccctctgt tgggaaatat aaaaatctac ttttcaatcc
     2641 ttttaactgg gccacttggt aggcttgcat tccttctgat tcaggtactc taaaatccat
     2701 tcctccatga ataaccataa ttggtgtatc ccaattttgc acatagcggt gcggtgaatt
     2761 ttttaagaat gcttctttgt tttcttgttg ccaatatggt ccgcccacat cccacgttgc
     2821 aaaaaacaac tcttcggtca tgccatacca gctttccaag ttaaacaaac cacaatgtgc
     2881 aatgaatgtt ttgaaacgtt tttggtgaac tcctgccaac atatacaccg aataaccacc
     2941 ataagaggcg ccaacagcac ccaatctatc tttgtcaaca tacggtaatt cagtagcagc
     3001 atcaattgct gatagataat cgttgatgga ttgacctccc caatcttttg aaatcgcatc
     3061 gttccattct tgtccaaaac caggtaaacc tctacgattt ggagcaacaa ctacatatcc
     3121 ttgagaagcc atcaccataa aattccagcg gtaagagaaa aactgactca cctcgctttg
     3181 tggtccacct tgacaataga gcaaagttgg atattttttg gttgcgtcaa agttgggcgg
     3241 aagaatcatc cataccaaca tatctttgcc atcggtagtt tt
//
LOCUS       DAOQVQ010000258         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_33787_length_3264_cov_3.710813, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000258 DAOQVQ010000000
VERSION     DAOQVQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33787_length_3264_cov_3.710813"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PKN22_07930"
     CDS             <1..2070
                     /locus_tag="PKN22_07930"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNS42673.1"
                     /translation="NICNPSGFDLTPYLPLTSVRVKANDLDGYGDNVTINASVTTIYN
                     VQDPAITINWYDAATNGTAQGTGHTINGLGTSVLPTAVDGTYQFYAESYLDGCVSTTR
                     TNVTLNVSSTHVELLPVDASCNNGADGSFVLSNVYCGTAPFTYAVDGASTFGAIPTDL
                     STGQHTVVVKDNAGNTSATYTITIGGAAGPSGVVVSSYNNDQATVNWVANGAETSWTI
                     EWGVPGFTPGTGTQIGTANAADTFYTITGLEGDSTYDIYVTTNCGSPYVDWGSTSVHT
                     DCDPMAALGYCESFEDINALGCWKVINANGDADTWAINTTPSYANTGVQSLNIYTDGN
                     SGNNDDYVLMPMMTLTGNEVLSFHYRVLSDSEPNDFEILLSTTGSNPADFVNTIYTDT
                     VSNEVYQDTSINLSSFTGDVYIAFHVPNGGLDGWRLLIDDVCINICTPVPGNDGMETV
                     CRSQNTIDLSTVITSPYSNGTWEFPANQSLINGSIMNISTLAAGTYHINYIVDGACTD
                     DTTIAVINVQNPSSAGTNGLITACKNQPIDLYGGLSGNVDFGGTWYRPNGTAMTGSYF
                     TTGTLVGQQVYKYVVTNGVCGADTAEVTVNIQNCDYAGLEDLSVLDNVTIAPNPNTGN
                     FQILGIPGADFTYEVLDLNGRLVVNTTNITSTITDVKLTDVQDGVYLVRIKGNNSERM
                     IRVIKQN"
     gene            complement(2185..2748)
                     /locus_tag="PKN22_07935"
     CDS             complement(2185..2748)
                     /locus_tag="PKN22_07935"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNS42674.1"
                     /translation="MKLVVGSKSPRRKQLLEEMGYSFEIRIKETDETFPDSYSPSQAV
                     CHIAEQKAKVLQTTLTNDEVLLTADTIVCVDQQILGKPKDVADAQRMLQLLSGRAHQV
                     ITAVCVATNTSLKTEFCTTQVTVKTLSEEEINYYINNYQPFDKAGSYGIQEWFGATSV
                     EKIEGSYNNVVGLPTHLVYQLLRPFKF"
     gene            complement(2745..>3264)
                     /locus_tag="PKN22_07940"
     CDS             complement(2745..>3264)
                     /locus_tag="PKN22_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNS42675.1"
                     /translation="KTLLMGNFFVSCIVTSAVIVVPLFMDADKIHYSYPHDTLDPVFQ
                     TFNWNVVWGFALLAFLQNLAREIIKDVEDMAGDKKINATTFPIVYGEKFAIEFFGIVT
                     LVYPLLLCYYFFAHSLHISVYLIPLIISAFINIFVGYLAFFDKSLKAKRWMKNFTKYA
                     MIIGLLYLFLPR"
BASE COUNT         1052 a          552 c          628 g         1032 t
ORIGIN      
        1 aatatttgta atccttcagg ttttgacttg acaccatact tgcctttaac ttctgttaga
       61 gtgaaagcaa atgatttgga tggatatggt gataatgtga caattaatgc aagtgttaca
      121 actatctata atgttcaaga tcctgcaatt actattaatt ggtatgatgc tgcaacaaat
      181 ggtacagctc aaggaactgg tcatacaatt aatggattag gtacatctgt attacctaca
      241 gcagttgatg gtacctacca attctatgca gagtcttacc ttgatggatg tgttagtaca
      301 acaagaacaa atgttacttt aaatgtttct agtacacacg ttgaattatt accagttgat
      361 gcgtcttgta acaatggagc agatggttca tttgtattaa gcaatgttta ttgtggtact
      421 gctccgttta cttatgctgt tgatggtgcg tctacatttg gtgctatccc tacagattta
      481 tctacaggtc aacacacagt tgtagttaaa gataatgcag gaaatactag tgcaacatac
      541 acgattacaa ttggaggtgc ggcaggtcca agtggagtgg tagtttcttc atacaacaac
      601 gatcaagcaa ctgtaaattg ggtagctaat ggtgcagaaa catcatggac aatagagtgg
      661 ggtgtgcctg gatttactcc tggtactgga acacaaattg gaactgcaaa tgctgctgat
      721 actttctaca ctattacagg tttagaagga gattctacgt atgatattta cgttacaact
      781 aactgtggta gcccttatgt agattggggt tcaacttctg tgcatacaga ttgtgatcca
      841 atggctgcat taggttactg tgaaagtttt gaagacatca atgctttagg atgttggaaa
      901 gtgattaacg caaacggtga tgcggatact tgggcaatta acacgactcc atcttatgca
      961 aacacaggtg ttcaatcatt gaatatttat acagatggta attcagggaa caatgatgat
     1021 tatgttttaa tgccaatgat gacattgaca ggaaacgaag tattgtcatt tcattataga
     1081 gttttaagcg attcagaacc taatgatttt gaaatcttat tgtcaactac aggatctaat
     1141 ccagctgatt ttgttaatac tatttatact gatacagttt caaatgaggt ataccaagat
     1201 acaagtatta acttaagttc atttactgga gatgtgtaca ttgcattcca tgttcctaat
     1261 ggaggattag atggatggag attattaatt gatgatgtat gtatcaatat ttgtacacca
     1321 gttccaggaa atgacggaat ggaaactgtt tgtcgttctc aaaatacaat tgatttgagt
     1381 acagttatca cgtctccata ttcaaatggt acatgggaat tcccagctaa ccaaagtttg
     1441 atcaatggtt ctattatgaa tatttctaca ttggctgcag gaacatacca catcaattat
     1501 attgtggatg gtgcttgtac ggatgataca acaattgcgg taatcaatgt tcaaaatcct
     1561 tcttctgctg gtacaaacgg tttaattaca gcttgtaaaa accaaccaat tgacttgtac
     1621 ggaggtttgt caggaaacgt tgactttggt ggtacttggt acagaccaaa cggaacagca
     1681 atgactggtt cttactttac aactggtaca ttagttggac aacaagttta caaatacgtt
     1741 gtaacaaatg gtgtttgtgg agctgataca gctgaagtaa ctgtaaatat ccaaaactgt
     1801 gattacgcag gtttagaaga cttgtcagta cttgataacg taactatcgc accaaaccct
     1861 aatactggaa acttccaaat cttaggtatc cctggagctg actttacgta tgaagtgtta
     1921 gacttgaacg gtcgcttggt agtaaataca actaatatta cttctacaat tacagatgtt
     1981 aaattaacag atgtacaaga cggagtttac ttagtacgta ttaaaggaaa taactctgaa
     2041 agaatgattc gtgtgattaa acagaattaa tctaatcaga taaaataatc caaaaggagg
     2101 ttgtcagaaa tggcaacctc tttttttgtt taatacatga ctagtcctaa ataaccgata
     2161 cagattattt aggactagcc acaattaaaa cttaaacggc cttaataatt gatacaccaa
     2221 atgtgttggc aatcccacta cgttgttata cgaaccttca attttttcaa cgcttgttgc
     2281 gccaaaccat tcttgaatac cgtaactacc tgctttatca aacggttggt agttgttgat
     2341 gtaataattg atttcttctt ctgaaagggt ttttacagta acttgagtag tgcaaaattc
     2401 ggtttttaaa gatgtatttg tagccacaca aacagctgta ataacttgat gcgctcttcc
     2461 ggataacaat tgcaacatgc gttgagcatc ggcaacatct ttgggtttac ctaaaatttg
     2521 ttggtcaaca cacacaatcg tatcggcggt taacaacact tcatcatttg ttaatgttgt
     2581 ttgtagcact tttgcttttt gctcggctat gtggcaaacc gcttgtgatg gtgaatagga
     2641 gtcggggaat gtttcatcgg tttcttttat tcttatttca aaagaatagc ccatttcttc
     2701 caacaactgt tttcttcggg gcgattttga gccaacaact aatttcatcg aggtaaaaac
     2761 aaataaagta aaccaattat catggcatat ttagtaaaat ttttcatcca gcgttttgct
     2821 tttaacgatt tatcgaaaaa tgccaaataa cccacaaaaa tattgatgaa tgcactgata
     2881 atgagtggaa ttaaatacac ggaaatgtgc agtgaatgtg cgaagaaata ataacacagc
     2941 aaaagcggat aaaccaaggt aacgatgcca aaaaattcta tggcaaattt ttcaccataa
     3001 acgataggaa atgtagttgc attgattttt ttatcaccag ccatatcttc cacatctttg
     3061 atgatttcgc gtgctaaatt ttgcaaaaat gccaacagag caaatcccca aaccacgttc
     3121 caattaaatg tttgaaaaac tggatctagt gtgtcgtgtg gataactata atgaattttg
     3181 tctgcatcca taaacaatgg aacaacaata accgctgagg taacaatgca agaaacaaaa
     3241 aagttgccca tcaacagtgt tttc
//
LOCUS       DAOQVQ010000259         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_33845_length_3261_cov_3.859638, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000259 DAOQVQ010000000
VERSION     DAOQVQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33845_length_3261_cov_3.859638"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKN22_07945"
     CDS             <1..1203
                     /locus_tag="PKN22_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS42676.1"
                     /translation="KETGAAIVYGPTAQPDFEAIIGEDNQLFELGDVKIKLLHTPGHT
                     LESSCFLLIDENGNETALFSGDTLFLGDVGRPDLAQKAANMTQDQLAGLLYDSLYTKI
                     LPLNDDIIVYPAHGAGSACGKNMMKETVDTLGNQKQMNYALNQPNKESFIKAVTDGLL
                     KPPAYFAMNVAMNKKGYKNFDNVLKSALTPIAAVDFEAQAEQTGALILDTRSDKEFTK
                     GFVPQSINIGLNGDFAPWVGALIIDVNQPILLVTEKGKEEETITRLSRVGFDNVIGYL
                     DGGFESWANANQEVDQVNRITAEQFSKDFYDNSKVIDVRKESEFNAEHVESAENLPLA
                     DINEWIHSINPNEHFYLHCAGGYRSMIAASILQARGYRNFTEVEGGFNAISLTNIPKT
                     NYVCQSKS"
     gene            1185..1562
                     /locus_tag="PKN22_07950"
     CDS             1185..1562
                     /locus_tag="PKN22_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS42677.1"
                     /translation="MSKQILKLIGYGLVAIIAINLFQNIYAQTDNGAVQKAIKEGAFL
                     VDVRTPQEFAEGSAKGAVNIPLNEINARLSEFKGKKSIVVFCRSGARSNQAKNILEQN
                     GYKNVINGGTWENVNKLTQGNKK"
     gene            1587..2549
                     /locus_tag="PKN22_07955"
     CDS             1587..2549
                     /locus_tag="PKN22_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNS42678.1"
                     /translation="MFKQKIPGIVLAVVLMLIAKMLSGVLPSLGTALIALILGIIVRL
                     IVVKFDRFTSGVVWSEKYILETAIVFIGFGFEISKFNEIGFSTLSIIVGSIVITILFA
                     LLLNKIVGNKNKKLFWLLGAGSAICGSSAIGATAPIIQSKEEETGASLAVINVMGLIG
                     MILLPTIATALNYSNLETGIFLGGILQSVGHVVGAGYAVNDEVGQFATVVKMGRVAFL
                     IPFLIILYFVFRKNSDGSKVKFPIFILFFLLAVIISQSTLIQPDALKLLSKSGDILLN
                     IGMAAIGLKIKLQSLWKISGKAFLSTSAIFFVQILIYMLYIAYL"
     gene            2725..>3261
                     /locus_tag="PKN22_07960"
     CDS             2725..>3261
                     /locus_tag="PKN22_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS42679.1"
                     /translation="MNINTYFRKKSIASINQCSLEDAHQNSLAKHLGVRDLTSLGIAA
                     IIGAGIFTTIGQASADGGPAVAFLFIFTSIACGFAAFAYAEFASVVPVSGSAYTYSYV
                     AFGELFAWVIGWALIMEYAIGNITVAIGWSGYFTELLASMGIHFPAWTCVDYFTASKG
                     FVEVQGLLASGVTMDSLTF"
BASE COUNT         1065 a          508 c          640 g         1048 t
ORIGIN      
        1 aaagaaacgg gtgctgcaat tgtatatgga cctacggcac agcccgattt tgaggcaatt
       61 attggagaag acaatcaact atttgaattg ggggatgtta aaattaaact tcttcacaca
      121 cctgggcaca cgcttgaaag ttcttgtttt ttattaattg atgaaaatgg aaatgaaact
      181 gctttgttta gtggcgacac attgttttta ggtgatgtag gaagacccga tttggcgcaa
      241 aaagcagcaa acatgacaca agatcaacta gcaggcttgt tgtatgatag cttgtacact
      301 aaaattttac ctttaaacga tgacattatt gtttatccag cgcacggtgc aggatcagct
      361 tgtggaaaaa acatgatgaa agaaacggtt gacaccttag gcaatcaaaa acaaatgaat
      421 tacgctttaa atcaacctaa taaagaatca tttattaaag ccgttacaga cggattatta
      481 aaaccacctg cttattttgc aatgaatgtt gctatgaata aaaaaggtta caaaaatttt
      541 gacaacgtat taaaaagtgc tttaacacca atagctgctg ttgattttga agctcaggca
      601 gagcaaactg gtgcattaat tttagacaca cgaagtgata aagaatttac aaaaggtttt
      661 gttcctcaat caataaacat tggtttaaat ggtgattttg caccttgggt tggcgcttta
      721 atcattgatg ttaatcaacc tattttgttg gtaacagaaa aagggaagga agaagaaaca
      781 attacacgat taagtagagt tggttttgac aatgtgatag gctatttaga tggcggattt
      841 gagagttggg ctaatgctaa tcaagaagtt gaccaagtaa atcgtatcac cgctgagcag
      901 ttttcaaaag atttttatga taacagcaaa gtaattgatg ttagaaaaga aagtgagttt
      961 aatgcagaac acgtagaaag tgctgagaat ttaccattag ccgacatcaa tgaatggatt
     1021 cattcgataa atccaaacga acatttttat ttacactgtg caggaggata cagaagtatg
     1081 atcgctgcaa gtattttaca agctagagga taccgcaatt ttacagaagt tgaaggtgga
     1141 ttcaatgcaa tttctttaac taatatacca aaaacaaatt acgtatgtca aagcaaatct
     1201 taaaattaat cggttatgga ttggtggcaa ttattgcaat caaccttttt caaaacattt
     1261 acgcacaaac ggataacggt gcggttcaaa aagcaataaa ggaaggtgct ttcttagtgg
     1321 acgttagaac gccacaagaa tttgccgaag gtagtgctaa aggagctgtc aacatccctt
     1381 taaacgaaat aaacgctcgt ttaagtgaat ttaaaggtaa aaaaagcatc gttgtgtttt
     1441 gtagaagtgg tgccagaagc aaccaagcaa aaaacatttt agaacaaaat ggttataaaa
     1501 atgttatcaa tggtggcact tgggaaaatg taaacaaact gacacaagga aataagaagt
     1561 agaaaacatt caaaaaatcc aatactttgt ttaaacaaaa aatcccagga atagtattag
     1621 cagttgtgtt aatgctaatt gcaaaaatgc tatcaggtgt attgccttca ttaggaacgg
     1681 cactcatcgc tttgatttta ggaataattg ttcgattaat cgttgtcaaa tttgaccgtt
     1741 ttacttcagg cgttgtttgg tcggaaaaat acattcttga aactgcaatt gtgttcattg
     1801 gttttggttt tgaaatttca aagtttaatg agattggatt ttctacactt tccataattg
     1861 ttggaagtat tgttattacc attctttttg ccctgctttt aaataaaatt gtaggaaata
     1921 agaataaaaa actattttgg ttacttggtg cgggaagcgc aatatgtgga tcatctgcaa
     1981 ttggagcaac cgcaccaatc atccaatcaa aagaagaaga aactggagcc tcactagctg
     2041 ttatcaatgt catggggctt attggaatga tattactacc tactattgct actgctctta
     2101 actattccaa tttagaaaca ggtatttttt taggtggaat tttgcaatct gttggtcatg
     2161 ttgttggtgc tggatatgcc gttaatgatg aagttggaca atttgcaaca gttgtcaaaa
     2221 tggggcgagt agcattttta attccttttt taattatttt atattttgtt tttcgaaaaa
     2281 acagcgatgg ttcaaaagtg aaattcccaa ttttcatttt atttttctta ttggctgtta
     2341 ttatttctca atccacactc atccaacccg atgcattgaa acttttgtca aaatctggtg
     2401 atattttatt aaacatagga atggcagcca tagggttaaa aataaaactt caatctcttt
     2461 ggaaaataag tggcaaagca tttctatcaa cctcagccat cttttttgtg caaattttaa
     2521 tttacatgct atacattgct tatttgtaaa ctaaataacc tatttaacaa aaagcagaag
     2581 tctatttatc aagtatattt tttccataat tttttcatta gcagaaactg atttactgaa
     2641 aatttgtatt tttcgggtca caagcaaact aacacaatca tttttaatcc atacaaaatg
     2701 attgaataag accgaaaaat caatatgaac ataaatacat atttcagaaa aaaaagcatt
     2761 gcaagcatca atcaatgttc actcgaagat gctcatcaga actctcttgc caaacatttg
     2821 ggcgttagag accttacatc tttagggatt gcagcgataa ttggtgcggg aatttttacc
     2881 accattggac aagcaagtgc agacggtggt cctgcagttg cttttttgtt cattttcact
     2941 tctattgctt gtgggtttgc cgcttttgcg tatgctgagt ttgctagtgt tgttcctgtt
     3001 tctgggagcg cctacaccta cagttatgtt gcatttggtg agttgtttgc ttgggtgata
     3061 ggctgggctt tgattatgga atatgccatt ggaaacatca ccgtggcaat tgggtggagt
     3121 ggctatttca cagaactact ggccagtatg ggaattcatt ttccagcttg gacttgcgtt
     3181 gattacttta ctgccagcaa aggttttgtt gaagttcaag gattgttggc ttctggtgtt
     3241 actatggata gcttaacatt t
//
LOCUS       DAOQVQ010000260         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34154_length_3243_cov_3.419072, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000260 DAOQVQ010000000
VERSION     DAOQVQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34154_length_3243_cov_3.419072"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKN22_07965"
     CDS             complement(<1..206)
                     /locus_tag="PKN22_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS42680.1"
                     /translation="MIPFSPPRIDQKVVDEVTAALLSGWITTGPRTKNFEKRITEYTG
                     CKATVAVNSWTMGMQVLLHWWGIK"
     gene            complement(287..727)
                     /locus_tag="PKN22_07970"
     CDS             complement(287..727)
                     /locus_tag="PKN22_07970"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNS42681.1"
                     /translation="MLSPFNDEYFMKQAILEAQKALEKDEVPIGAVIVANNQIISRGH
                     NLTECLNDTTAHAEMQAITAASGNIGAKYLNECTLYVTMEPCVMCAGALYWSQIDRIV
                     FGASDEKRGAGRFAGIYHPKTKVEGGLLAEQCSKLVVDFFKSKR"
     gene            846..2132
                     /locus_tag="PKN22_07975"
     CDS             846..2132
                     /locus_tag="PKN22_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42682.1"
                     /translation="MKKKINTLISLSSGIVLLVILWGLYASLFLYSFQSKAPEHHFVV
                     PENASLVVELDGRDVLFDFLNSTFIKGEGDEIVQLIKNSNKKTDETQQFGINWLQPVI
                     YFQSTYKQQPVKGIIVQIINAHEWKNNSTNFFNQRCVSQSDENSGIIVHSNQLSEKEL
                     QQFAQQLYKKEVLVGKKLADNHIVQLKNNSNNWQSNLDVQINDTAIVSKGSVSLDKSY
                     TSTYVQYKLTPADFHFTTDVITDQLNDSLQKLIGTNLALTGVSINYRGIIVDNINNEM
                     ALIPDADFVLSFKQATTMEQFVSNIPNAVFSFDKETVTIGKRIFHIRQLSKNSIYFGK
                     TKAPELHPNRQHVGLYIKGALKPLITIEGSKLVQAIVRMSAPYVLATELSNEVVNCTI
                     ALTSTGKNTYDLDAAFRFKGKEARLSLLKVVLKAAQ"
     gene            complement(2188..>3243)
                     /locus_tag="PKN22_07980"
     CDS             complement(2188..>3243)
                     /locus_tag="PKN22_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS42683.1"
                     /translation="FNFPVAVWSWNTALAWVCGDVTIWKPSEKTPITALACQQIIKQV
                     FDANAVPEGVSNLVIGDATLGKLMAEDKRVPLVSATGSTRMGKSVGAAVGARLGRSLL
                     ELGGNNAIIITPSADLKMVVPGAVFGAVGTCGQRCTSTRRLIIHEEVYDKVKEAMVKA
                     YGQLKIGNPLDQNNHVGPLIDQLAVDAFTNALKQVKEEGGTVITGGEVLTGEGYESGM
                     YVKPAICEVQNHFQIVQHETFAPILYLIKYKGGVENAIAIQNDVPQGLSSAIMTNNLK
                     EAEKFLSQAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKVYMRRQTNT
                     INYSDSLPLAQGIKFDL"
BASE COUNT          991 a          664 c          568 g         1020 t
ORIGIN      
        1 tctttaattc cccaccaatg caacaatact tgcattccca ttgtccacga gtttacagca
       61 accgttgctt tacaacctgt gtattctgta attcgttttt caaagttttt ggtacgtgga
      121 cctgttgtta tccatccgct taacaaagca gcggttactt catctacaac tttttggtcg
      181 atgcgtggtg gtgaaaaagg aatcataatt tattgatttt attgttgcga aagtagtaaa
      241 aaactacatt gattgttgtg ttgaatgttg aatttataaa aaaatactag cgcttggatt
      301 tgaaaaaatc aaccaccaat ttactgcatt gttcggccaa taaaccgcct tcaacttttg
      361 tttttggatg gtaaattccg gcaaaccttc ctgctcctct tttttcatcg cttgctccaa
      421 aaacaatacg gtctatttgc gaccaataca acgctccggc acacattacg caaggctcca
      481 tggtaacata caacgtacat tcgtttaaat attttgctcc aatgttgcca cttgccgcag
      541 ttatggcttg catttcagca tgagcggtag tatcgttcaa acattctgtc aagttgtggc
      601 cgcgtgaaat gatttgatta ttggcaacaa ttaccgctcc aatgggcact tcatcttttt
      661 caagtgcttt ttgtgcctct aggatggctt gtttcatgaa atattcgtcg ttgaacggag
      721 aaagcataac tttttagtgt cgaaattacg ttatttatgc tgattttggc acaaaaatga
      781 acgatttcaa taaaatttta taaaaataaa gagctaacat aagagtttct ctatctttgt
      841 tttgcatgaa aaagaaaata aatacactta tttcactcag ttcgggcatt gtgctcttgg
      901 tgattttgtg gggattgtat gcctctttgt ttttatattc ttttcaaagc aaagctcccg
      961 aacatcattt tgtggtgcca gaaaatgctt cgttggtagt tgaattggac ggacgcgatg
     1021 tgttgtttga ttttctcaac agtactttta tcaaaggtga aggcgatgaa attgtgcaac
     1081 tcattaaaaa cagtaacaaa aaaacagatg aaacccagca atttggtatc aattggttgc
     1141 aacctgttat ttattttcaa tcaacttata agcagcaacc tgtaaaagga ataattgtgc
     1201 aaatcatcaa tgcccatgaa tggaaaaaca atagcacaaa cttcttcaac caacgttgtg
     1261 tttcacaatc cgacgaaaat tcgggtatta ttgttcattc caaccaactt tctgaaaaag
     1321 aattgcaaca atttgctcaa caattgtata aaaaagaagt gttggtgggt aaaaaattag
     1381 ccgacaacca catcgttcag ctaaaaaaca attccaacaa ttggcaatcc aacttagatg
     1441 tgcaaataaa cgatacagcg attgtttcaa aaggtagtgt atcgcttgat aaatcgtaca
     1501 catcaactta tgtgcaatac aaattaacac ccgctgattt tcattttact acggatgtaa
     1561 ttacagacca gctcaacgat agtcttcaaa agctcattgg aaccaattta gcattgacgg
     1621 gtgtgagtat taattaccga ggaattattg tggacaacat caacaatgaa atggcattga
     1681 ttccagatgc cgattttgtg ttgagtttta agcaagcaac aacgatggag caatttgtga
     1741 gtaatattcc caatgctgtt ttttctttcg ataaagaaac agtaaccatt ggcaaacgta
     1801 tttttcacat tcgccaattg tctaaaaact ctatttattt tggaaaaaca aaagctccag
     1861 agcttcatcc caaccgtcag catgttggtt tatacataaa aggagcgttg aaaccgttaa
     1921 ttacgattga gggtagcaaa ttggtacaag caattgtgcg catgagtgct ccctacgtgt
     1981 tggctacaga attatcaaac gaagttgtca attgcaccat tgcattaacc tctactggaa
     2041 aaaacacgta tgatttagat gcggcttttc gattcaaggg caaagaagct cgtttatcac
     2101 ttttaaaagt agtgttaaaa gctgcgcaat aaaaaaatgg gatgtcgttt gcacaacacc
     2161 ccatttcgta tccattcaat tcttgtttta caaatcaaat ttaattcctt gtgctaatgg
     2221 caagctatcg gaatagttga tagtatttgt ttgacggcgc atatacactt tccaagcgtc
     2281 agaacctgat tcacggcctc cacctgtttc tttttcgcca ccaaaggcac caccaatttc
     2341 agcacccgaa gtaccgatgt ttacattggc aataccacaa tctgaaccag cttgcgacaa
     2401 gaatttttct gcttctttta aattattagt cataattgca gaagacaaac cttgcggcac
     2461 atcgttttga atagcaattg cattttcaac accacctttg tatttaatta aatataaaat
     2521 tggtgcaaat gtttcgtgtt gaacaatttg gaaatgattt tgaacctcac aaattgcagg
     2581 tttcacatac attccagatt catatccttc gccagttaaa acttcaccac ctgtgatgac
     2641 agttccacct tcttctttta cttgtttcaa cgcatttgta aacgcatcaa cagccaattg
     2701 gtcgataagc ggtccaacat ggttattttg atccaatggg ttaccaattt tcaactgacc
     2761 ataagctttc accattgctt cttttacttt gtcatatact tcttcatgga tgattaaacg
     2821 acgtgtagat gtacaacgtt gtccacatgt tcccacagca ccaaatacag cacctggaac
     2881 aaccattttc aaatcagcac ttggcgtaat gatgatggca ttgttaccac ccaactccaa
     2941 taaagagcga cccaagcgtg cacccactgc agcacctaca gatttaccca ttcttgttga
     3001 acctgttgca gaaactaacg gaacgcgttt atcctctgcc attaattttc ctaatgttgc
     3061 atcgccaatt accaagttag aaacaccttc tggtaccgca tttgcgtcaa aaacttgttt
     3121 aataatttgt tgacaagcaa gcgctgtgat aggtgttttt tcggatggtt tccaaattgt
     3181 aacgtcacca caaacccaag ccaatgctgt gttccagctc caaacggcaa ctgggaagtt
     3241 gaa
//
LOCUS       DAOQVQ010000261         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34160_length_3243_cov_2.861982, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000261 DAOQVQ010000000
VERSION     DAOQVQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34160_length_3243_cov_2.861982"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1223)
                     /locus_tag="PKN22_07985"
     CDS             complement(120..1223)
                     /locus_tag="PKN22_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNS42684.1"
                     /translation="MFIDVLQQRILGNTVEDYLWFAGLILAGLIFKNLISRILTFLLF
                     NVLRKYFKTIGVKKFVELTQKPFSTTLFLIFIYIACRYVHFPDEWKLASESEFGVRMT
                     IKLIFQIATVFFITWTLLRIIDYIGLVMRARANKTESKMDDLFVPFFVSSIKALTVII
                     SLFFILGVVFKLNVASIIAGLGIGGLAIALASKDTLENLLGSFLIFMDKPFVVGDLVK
                     TDNLRGHIEEVGFRSTKVRTIEKTLVIVPNKKMMDAELENQTERTFYRHRFVIGLVYS
                     TTADQIQTISQQILDMLNEHPLVNENPNVYFTNFGASALEIEVIYFVKTQELSDFENT
                     RQEVNFAIMKIVAANKSDFAFPSTSVYIEKEVK"
     gene            complement(1223..2449)
                     /locus_tag="PKN22_07990"
     CDS             complement(1223..2449)
                     /locus_tag="PKN22_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNS42685.1"
                     /translation="MLDLLKKFEEKRPEIVFEWSDAETGAEGWLVINSLRNGSAGGGT
                     RMRKGCDKREVESLAKTMEVKFTVSGPPIGGAKSGINFDPADPRKNQVLERWFKAIKP
                     LLKSYYGTGGDLNIDEIHDVIPICNKLGILHPQEGTAVGHFNDGEENIKKRISNLQKG
                     VSLELKDSNLSPDLDNKYRVADMITGYGVAYAVKYYYELWGGKISEKRAIIQGWGNVA
                     AAAGFYLAQMGVKIVGIIDRDGGLINEKGYSFEEIKTLFNNRKGNKLHADNMLSPQQM
                     AEKVWNLDAEIFIPGAASRLVTQEQMQSLISKKLEVISCGANVPFADKEIFFGPIGMY
                     CDKHVSVIPDFIANCGMARVFAYLHNANAVIDDRLIFDDAANTIKNALQEVIATHKDK
                     KGIASAAFEIALKKLL"
     gene            2583..2765
                     /locus_tag="PKN22_07995"
     CDS             2583..2765
                     /locus_tag="PKN22_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42686.1"
                     /translation="MRNQRVEQKKSIKDNWETDNYHAKKHKMSNHREEKNWKNKILKE
                     EEEVPDEDFFRFDDEE"
     gene            complement(2802..>3243)
                     /locus_tag="PKN22_08000"
     CDS             complement(2802..>3243)
                     /locus_tag="PKN22_08000"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNS42687.1"
                     /translation="NTALFDKLNALEFYRTPYPRSLGAEWFNEYFLPVLNASSASLTD
                     KTTTVTHHIAKQIGDSLTISDNKLKSTLLVTGGGSKNKFLVQKIQENTSVHIIIPDEK
                     IIDFKEALIFALLGYLRINNHKNTLATVTGAKKDSVGGAVYLMA"
BASE COUNT         1060 a          622 c          529 g         1032 t
ORIGIN      
        1 tttatggtta aaagaaactt ttaaagcagc ctgatttttc taggggaaat agatagcgtg
       61 ggtattttct ttcttgggag aattttaaaa actgttactc aatgccccaa aatggttggt
      121 tatttcactt ccttttcaat ataaacagaa gtgctgggaa atgcaaagtc acttttattg
      181 gctgccacaa ttttcataat ggcaaaattg acttcttgtc ttgtgttttc gaaatcgctc
      241 aactcttgag ttttaacgaa ataaataacc tcaatctcta aagcacttgc cccaaaattg
      301 gtaaagtaaa catttggatt ttcattaacc aatgggtgct cgttaagcat atccagaatt
      361 tgttgcgaaa tggtttgaat ctgatcggct gttgtagaat ataccaaacc aataacaaac
      421 ctatggcggt aaaaagttct ttcagtttgg ttttctaatt cagcatccat cattttttta
      481 ttcggaacta tcaccaatgt tttttcaatg gtacgtactt ttgttgatcg aaatccaacc
      541 tcttcaatat ggccacgaag attatcggtt ttaactaaat cgcctaccac aaaaggcttg
      601 tccataaaaa ttaagaaaga acctaacaga ttttctagtg tatctttaga agccaaagca
      661 atggccaaac caccaatacc taaaccggca attatgctgg ctacattgag tttaaacaca
      721 acgcccaaaa taaaaaacag actgatgata actgttaaag ctttaatgga ggatacaaaa
      781 aaaggaacaa acaaatcgtc cattttcgat tctgttttgt tggcacgtgc acgcattacc
      841 aaaccgatgt aatcaattat ccgaagcaat gtccaggtga taaaaaagac tgtggcaatc
      901 tgaaaaatca atttaattgt cattcttaca ccaaactcac tctctgaagc aagtttccat
      961 tcatcaggaa aatgcacata acgacaagca atgtaaatga aaatcagaaa aagtgttgta
     1021 gagaaaggtt tttgtgtcag ctctacaaat ttttttacac caatggtttt aaaatatttt
     1081 cttaaaacat tgaagagtaa aaaagtcaga attctggaaa taagattctt gaaaataagt
     1141 cctgccagaa taagtcctgc aaaccacaga taatcctcaa cggtgtttcc taatatcctt
     1201 tgttgcagaa catctataaa cattacagca gttttttaag agctatttca aaagcagcag
     1261 aagcaatacc ttttttatct ttatgtgttg caatcacttc ctgcaatgca tttttgatag
     1321 tgtttgctgc atcatcaaaa atcaatctgt catcaataac ggcatttgca ttgtgcaagt
     1381 aagcaaaaac tctagccata ccacaattag caataaagtc gggaataacg cttacatgct
     1441 tgtcgcagta cattccaatg ggaccgaaga aaatctcttt atcggcaaat ggtacgttgg
     1501 caccacatga aatgacttca agttttttgc tgatgagcga ttgcatttgt tcttgcgtta
     1561 ccaatcgcga agcggcaccg ggaataaaaa tttctgcatc aaggttccac actttttctg
     1621 ccatctgttg tggtgatagc atattatcag catgcaattt gtttccctta cgattgttga
     1681 ataaagtttt tatttcttca aaggaatatc ctttttcgtt gattaaaccg ccatctctgt
     1741 caatgattcc aacaattttt acacccattt gtgcaagata aaaaccggca gcagctgcaa
     1801 cattacccca tccctgtatg atagctcttt tctcagaaat ttttccaccc cacaattcat
     1861 agtaatattt tacagcataa gctacaccat agcctgtaat catatcggct actctgtatt
     1921 tgttgtctaa atcaggtgac aagttcgaat ctttcaattc aagagataca cctttttgca
     1981 gattgctgat acgtttttta atattttctt ctccatcgtt aaagtgacct acagcagtgc
     2041 cttcctgcgg atgtaatatt cctaatttat tgcagattgg aatcacgtca tgaatttcgt
     2101 caatgtttaa atcaccaccg gttccataat atgatttcag caatggcttg atagctttaa
     2161 accagcgttc aagaacctga ttttttctgg ggtcagcagg gtcgaagtta atacccgact
     2221 tagcaccacc aattggagga cctgacaccg taaacttcac ctccatagtt ttggcaagcg
     2281 attccacttc tctcttatca catcctttac gcatacgagt gccgcctccg gcagagccat
     2341 tacgcagact gtttataacc agccaccctt cagcgcctgt ttcagcatca ctccattcaa
     2401 atacaatttc aggacgcttt tcttcaaatt tctttagtaa atccagcatt atgtttaaaa
     2461 aatttgcacg aaaatattaa aattttccgc ttaaaaccct tgaaaaaaac ttctgtttct
     2521 gaaacctcaa caacagtttt acagtaaaat agtttattat tctaattttt attttcaatg
     2581 acatgagaaa tcaaagagtt gaacaaaaaa agtcaataaa agacaattgg gaaacggaca
     2641 actatcatgc aaaaaagcat aagatgtcta accatcgtga agaaaaaaac tggaagaaca
     2701 aaatcttgaa ggaagaggaa gaggttcctg atgaagattt tttcaggttt gatgatgagg
     2761 aataatccca acggataaga ttcttaatta aagtttgtgg tttaagccat taagtaaacg
     2821 gcaccaccaa cagaatcttt ttttgcacct gttacagtgg caagtgtgtt tttatggttg
     2881 tttattctca aatatcctaa aagtgcaaaa atcaatgctt ctttaaaatc aattattttt
     2941 tcgtcaggaa taataatatg tactgatgta ttctcctgaa ttttttgtac aagaaattta
     3001 ttctttgatc caccaccggt aactagtaaa gtagatttta acttattgtc cgaaatggtt
     3061 aatgaatcac caatttgttt tgcaatatga tgtgttactg ttgttgtttt atcagtcaat
     3121 gaagctgaag atgcatttaa aacaggcaga aaatattcat taaaccactc agcacctaaa
     3181 gaacgcggat aaggtgtacg ataaaattct aatgcattta gcttatcaaa tagtgctgta
     3241 ttc
//
LOCUS       DAOQVQ010000262         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34320_length_3233_cov_3.488357, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000262 DAOQVQ010000000
VERSION     DAOQVQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34320_length_3233_cov_3.488357"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKN22_08005"
     CDS             <1..920
                     /locus_tag="PKN22_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS42688.1"
                     /translation="CLAYPHIELLFIFLLIRFAHGFSAGFFPTSATAMATDLLPDDKR
                     GQGMGIWGIFISVGFGLGQMLTQPLVAMFGYNSLFYVAFGITLLAAILILPIQETLHE
                     KQRVAFNPQLLMVKANDVVDPPVRPAAIVMLLASTCSGFVYTITPDIAQFLALENKGI
                     FFGIYAISSLVVRLFTSSLSDRIGRRKTLVVAVIVLFFAMLMTGFADNPHTFYIAAIL
                     YGFASGISSPALMAWMADLSLPHRRGVGSGTLYIALEGGFMLGAGLSILFYDSTKASV
                     LTTFIVASTFAFLALLYLIWHLITFKESK"
     gene            complement(912..1217)
                     /locus_tag="PKN22_08010"
     CDS             complement(912..1217)
                     /locus_tag="PKN22_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNS42689.1"
                     /translation="MEELENKIIEVLKTIYDPEIPVDIFELGLIYEVNIDDDKNVEIV
                     MTLTSPNCPVAESLPKEVEDKVGEIEGITTAKVAVVFDPPWDKDMMSEVAQLELGLL"
     gene            complement(1220..1633)
                     /locus_tag="PKN22_08015"
     CDS             complement(1220..1633)
                     /locus_tag="PKN22_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNS42690.1"
                     /translation="MTLEGKQQEIIDEFSIYSDWMEKYEYIIELGKNLPIIDEDKKTE
                     GRLIEGCQSRVWVADEFRDGKMYFTADSDAIITKGIIALLIQVLNEETPEAIVNADLH
                     FIKDIGLQDHLSPTRANGLLSMVKHLKKSALIALK"
     gene            complement(1674..2891)
                     /locus_tag="PKN22_08020"
     CDS             complement(1674..2891)
                     /locus_tag="PKN22_08020"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNS42691.1"
                     /translation="MGFSVEEVRKQFPTLHQEVYGKPLIYFDNGATSQKPQVVIDVIE
                     HYYAKENANIHRGVHYLSQKATDAYEEARRIVQRFIGAKHEYEVLFTKGTTDSINLVA
                     YSFGELLTAGDEIIISEMEHHSNIVPWQLLCERKKSVLKVAPVLENGELDLAAYEKML
                     SPKTKLVAITHASNTLGTINPIKQMVQQAHAVGAKFMVDGAQSIQHLKIDVQALDCDF
                     YAFSSHKVFGPTGAGVLYGKEDVLNAMPPYQGGGDMIDKVTFEKTTFNVLPFKFEAGT
                     PHIIGGIATGVALNYLSQLDLDAIHQHESALMHYAQTEMQKIEGLKIIGTAANKTSVI
                     SFVVDGVHPFDIGTLLDKQGIAVRTGHHCTQPLMDKFGIVGTTRASFAFYNTIEEVNV
                     FLNALKRSLELLK"
     gene            3070..>3233
                     /locus_tag="PKN22_08025"
     CDS             3070..>3233
                     /locus_tag="PKN22_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNS42692.1"
                     /translation="MNIENTQAQMKKGILEFCILSILKRKEAYPSEILDELKEARLIV
                     VEGTLYPLLTR"
BASE COUNT          904 a          630 c          550 g         1149 t
ORIGIN      
        1 cttgtttagc ctaccctcac attgagcttt tatttatttt cttgttgatt cgttttgcac
       61 atggttttag tgctggtttc ttcccaacga gtgccactgc aatggcaaca gatttacttc
      121 cagacgacaa acgtggtcaa ggaatgggta tttggggaat ttttatttcg gtgggttttg
      181 gtttgggaca aatgttaaca caacctttgg tggcaatgtt tggctacaat tcactttttt
      241 atgtggcctt tggaataacg ttgctggctg cgatattgat tttaccaatt caagaaactt
      301 tacatgaaaa acaacgggta gcgtttaatc ctcaattatt aatggtgaaa gccaatgatg
      361 tggttgaccc tcctgtgcga ccagcagcca ttgtgatgtt gcttgcctca acatgttctg
      421 ggtttgtgta caccattaca cccgatattg ctcaattttt agctcttgaa aacaaaggaa
      481 ttttctttgg aatttatgcc attagttcgt tggttgtccg cctgtttacc tcttcacttt
      541 cggatagaat aggcaggaga aaaaccttgg ttgttgcggt tattgtttta ttctttgcaa
      601 tgctaatgac aggttttgcc gataatccac atacatttta catagcggca attttatatg
      661 gttttgcatc tggaatatcg agcccggctt taatggcatg gatggccgat ttatcattac
      721 ctcatcgtag aggtgttgga tcgggaacgt tgtacattgc tttggagggt ggttttatgt
      781 tgggcgcagg tttgtctatc ttattctatg atagcacaaa ggcaagtgtt ttaacaacat
      841 ttatagttgc atcaacattt gccttcttag cattacttta tttaatttgg cacttgatta
      901 ccttcaagga atcaaagtaa acctaattct aattgagcca cttcgctcat catgtcttta
      961 tcccaaggtg gatcaaaaac cactgcaact ttagctgtag ttatcccttc tatctcacct
     1021 actttgtctt ccacttcttt tggtaaagat tctgctactg gacagttagg tgatgtcaaa
     1081 gtcataacga tttccacatt tttatcgtcg tcaatattca cttcataaat caatcccaat
     1141 tcaaaaatat ccacaggaat ttctggatcg tagatggttt ttaatacctc aattatttta
     1201 ttttctaact cttccataac tattttaaag cgattaacgc tgattttttt aaatgtttaa
     1261 ccatactcaa caaaccatta gcacgtgtag gcgataaatg gtcttgcaag ccaatatcct
     1321 taataaaatg taaatctgca ttaacaattg cctcgggtgt ttcttcgttc aaaacttgaa
     1381 ttaacaaagc aattattccc tttgtaataa tcgcatccga atcggctgtg aaatacattt
     1441 ttccgtctct aaattcatct gccacccaaa cgcgcgattg acatccttca atcaagcggc
     1501 cttctgtttt tttatcttcg tcaataattg gtaaattttt ccctaattcg atgatgtatt
     1561 cgtatttctc catccaatca gaataaattg aaaattcatc aattatttcc tgttgttttc
     1621 cttctaatgt catttttatt gaattgcact tttcttttca actacaaaaa taactatttc
     1681 aaaagttcta aacttctttt tagtgcattt aaaaatacat tcacctcttc aatggtgttg
     1741 taaaatgcaa aggaagctct tgttgtgcct acaattccaa atttatccat caagggttgt
     1801 gtgcagtggt gacctgttct aacggctata ccttgcttat ccaacaacgt tccaatatca
     1861 aacggatgta cgccatccac cacaaaagaa ataacgcttg ttttgttggc agctgttcca
     1921 attattttca agccttcaat tttttgcatt tccgtttggg cgtaatgcat taatgctgat
     1981 tcgtgttggt gaatggcatc taaatccaat tgcgataaat agttcaacgc cacacctgtt
     2041 gcaattccac caattatatg cggcgttccg gcttcaaact taaaaggtaa cacattaaac
     2101 gttgtttttt caaacgtaac tttatcaatc atatcaccac caccttgata gggaggcatg
     2161 gcatttaata catcttcttt tccataaagc accccagcgc ctgttggtcc aaatactttg
     2221 tggctggaaa aagcataaaa atcacaatcc aatgcttgca cgtcaatttt taaatgttga
     2281 atagactgtg ctccatccac cataaattta gctccaacag catgtgcttg ttgcaccatt
     2341 tgtttgatgg gattaattgt acccagtgta ttgcttgcat gagtaatggc aaccaatttt
     2401 gttttgggcg ataacatttt ttcatacgct gcaagatcca actcaccgtt ttcaagtaca
     2461 ggcgctactt tgagaaccga ttttttgcgt tcacacaaca actgccaagg aacaatgttg
     2521 gaatgatgtt ccatttcact gattatgatt tcatcgccag cagttaaaag ttcaccaaaa
     2581 ctataggcta ccaagttaat actgtcggtt gtgccttttg taaacaatac ttcgtattcg
     2641 tgttttgctc caataaatcg ttgcactatt cttcgcgctt cttcgtaggc atccgttgct
     2701 ttttgactca agtaatgcac cccacgatga atgttggcat tttcttttgc ataataatgt
     2761 tctatgacat caattaccac ttgcggtttt tgcgacgttg ctccattatc aaagtatatc
     2821 aacggtttgc catacacttc ttggtgaagc gtaggaaatt gttttctgac ctcctcaaca
     2881 ctgaatccca tttctagttt attaaaattc tatgcaaagg tacgtatttt gatttgtatt
     2941 gaatcattct aaataagtta aaatttattt aactgtattt caatgtttta aataatattt
     3001 taaaaatatt tagtactatg ttatacatac tactgttttt tatatatatc tttgcataaa
     3061 ataattaata tgaatatcga aaatacacaa gcgcagatga agaaaggaat tttggaattc
     3121 tgcattttat cgatattaaa aagaaaggaa gcatatcctt cagaaatatt ggatgagtta
     3181 aaagaagctc gcttgatagt ggttgaagga actctttatc cgttgttaac gcg
//
LOCUS       DAOQVQ010000263         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34477_length_3223_cov_4.728220, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000263 DAOQVQ010000000
VERSION     DAOQVQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34477_length_3223_cov_4.728220"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKN22_08030"
     CDS             <1..309
                     /locus_tag="PKN22_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNS42693.1"
                     /translation="TVFLAQMCGVNMSIGEQIGICLVLMLTSKGVAGVRGASFVILAG
                     AATTIEGVTIDKVSVILAVDALMDMARTSVNVLGNCLATVVIAKSEGEFHPSALKEEV
                     "
     gene            397..2604
                     /locus_tag="PKN22_08035"
     CDS             397..2604
                     /locus_tag="PKN22_08035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS42694.1"
                     /translation="MAIIGKIREKSWLLVGLVGLAMFAFILTDYNKGMFGGKEQIGYG
                     TIDGEVIDPKLYEEASRNYQQADQQEFAQQGREYTVRDQVQSDNKAWNAIVDSIILQK
                     EFNALGLSVSEREFDAFLYGHDGFTVLSSIAESFKDPATGLYSEKLLRKRVDELKNSS
                     KAEEQQAWNQTKVSLTLQRLQGKYFQLLSQGAYATNLEAKEQYIAQKRIKDISFVLKR
                     FSEIPDADIKITDEELRKYYEKNKDDVKYRVKSNSRDIKYFTVDIAPSKADSAAFNNE
                     LTKLKEGFAKQTSAKNDSIFVVKNSEWPYYVPKAGFRSENTPKANPNFTYPAQMDSVF
                     AAAGVGTVVGPYLDKGKYRLAKVLGVETTIYSVRHILLSAQKTDTLGSKAKIKQADSL
                     LQVINKDNFESFVTQYSQDPGSAKEGGKIENFIYSEMVPEFSDFAKNNPVGKIGRVQS
                     DFGVHIIEVLEKKAGRVPNLAIIEKTLKPSANTMDDINLLANDLVAEVVDVVDAKTTL
                     KAKSDAFDTIAIRKGYTPLSMTIDEKNTQVYGMKTPYAEDRLITLAYSDKSEAGMVNL
                     SPIKDEDRYIIAMLSVVREKGVPSFEAAEMAMRRELMNDKKANIFKSKLTGKSLEGIA
                     SKNGLEIQKAEITFANPSIMGGGYDPEIVGALYSKGLKDGMTTLPLKGRNAVYVVRVN
                     KTIAEPATKTYETEKQQLVGQARSSMQNDAITGLREKVNVVDNRRFLKIGIRH"
     gene            2750..>3223
                     /locus_tag="PKN22_08040"
     CDS             2750..>3223
                     /locus_tag="PKN22_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42695.1"
                     /translation="MKKLILSLFIAGGVGFGFSQMTVTGVSPASIVGNYTFSYGETTS
                     GWGSTMNFSTPGVHVQGYLALVEDGTTGTNAQGHPISQEGCSPLTNGSSVTGKIAVVY
                     RNTCEFGDKAKNAQDAGAIAVIVVNRDNEVIAMGGGTQGPNVTIPVVMLTSLDGAL"
BASE COUNT         1141 a          489 c          648 g          945 t
ORIGIN      
        1 accgtgtttt tagcacaaat gtgtggtgtt aatatgagca ttggagaaca aattggaata
       61 tgtttggtgt taatgctaac ttctaaagga gttgcaggtg ttcgtggtgc ttcttttgtg
      121 atattagccg gagctgcaac aacaattgag ggagttacga ttgataaagt cagtgtgatt
      181 cttgcagttg atgcattaat ggatatggca cgcaccagtg ttaatgtatt gggcaactgt
      241 ttggcaactg ttgtgattgc aaaatcagaa ggagagtttc atccaagcgc tttaaaagaa
      301 gaggtttaaa acttgaaata acaaaaattt acaaaccgaa atttttattt atcaaatcta
      361 ctgtttatat tcgcaccctt gtaattacaa tatcttatgg caataattgg aaaaattcgt
      421 gagaaatcat ggttattagt tggtcttgtt ggactagcaa tgtttgcttt tattcttact
      481 gactacaaca aaggaatgtt tggtggaaaa gagcaaattg gttatggaac aattgatgga
      541 gaagttatcg atccaaaatt gtatgaagaa gcaagtagaa actaccaaca agcagaccag
      601 caagagtttg cgcaacaagg aagagaatat actgtaagag atcaagtaca gtcagacaac
      661 aaagcgtgga atgcaattgt tgactcaatt attttgcaaa aagaattcaa tgcacttggc
      721 ttatccgtaa gtgagagaga attcgatgca ttcttgtatg gtcacgacgg ttttactgtt
      781 ttatccagca ttgcagaaag ttttaaagat cctgcaactg gtttatacag tgaaaaattg
      841 ttacgcaaac gtgttgatga attgaaaaac tcttcaaaag cagaagagca acaagcatgg
      901 aatcaaacaa aagtatcgtt gactttacaa cgtttgcaag gaaaatattt tcaactattg
      961 agtcaaggtg cttatgctac aaaccttgag gcaaaggaac aatatattgc tcaaaaaaga
     1021 attaaagaca tttcatttgt tttaaaacgt ttttctgaaa ttccagatgc tgatattaaa
     1081 atcaccgatg aagaattaag aaaatactac gaaaaaaata aagacgatgt taagtataga
     1141 gtgaaatcaa attcacgcga tattaaatat ttcacagtag atattgcacc ttctaaagct
     1201 gactcagctg catttaacaa tgaattgaca aaattaaaag aaggttttgc aaaacaaaca
     1261 tcagcaaaaa acgactctat ttttgttgtt aaaaacagtg agtggccata ttacgtacct
     1321 aaagcaggtt tccgttcaga aaacacacca aaagcaaatc caaactttac atacccagca
     1381 caaatggatt ctgtttttgc agcagcagga gttggaacag ttgttggacc ttatttagac
     1441 aaaggtaaat accgtttggc aaaagtatta ggtgttgaaa caactatcta cagtgttcgt
     1501 cacattttat taagtgcaca gaaaacagat acactaggaa gtaaagcaaa aatcaaacaa
     1561 gcagactctt tgttgcaagt aatcaacaaa gataattttg aatcatttgt tacacaatac
     1621 agccaagacc caggttctgc aaaagaagga gggaaaattg aaaacttcat ttatagcgaa
     1681 atggttccag aattcagtga ttttgctaaa aacaatcctg ttggaaaaat tggtcgtgta
     1741 caatcagact ttggtgttca catcatcgag gttttagaga aaaaagctgg tagagttcca
     1801 aacttggcaa ttattgaaaa aacattgaaa ccaagtgcta acactatgga cgatatcaac
     1861 ttattggcaa atgatttagt tgctgaagtg gtagatgttg tggatgcaaa aactactttg
     1921 aaagcaaaat cagatgcctt tgatacgatt gctatcagaa aaggatatac acctttgagc
     1981 atgacaattg atgaaaaaaa cacacaagtg tatggtatga aaactcctta tgcagaagat
     2041 cgtttaatca cattggcata tagtgataag agtgaagctg gaatggtgaa tttatctcca
     2101 attaaagatg aggatcgtta catcattgca atgctttctg ttgtaagaga aaaaggtgtt
     2161 ccttcatttg aagcagctga aatggctatg cgtagagagt tgatgaacga taaaaaagca
     2221 aacattttca aaagcaaatt gactggaaaa tcattagaag gaattgcttc taaaaatggt
     2281 ttagaaattc aaaaagccga aattacattt gccaacccat ccattatggg tggtggttat
     2341 gaccctgaaa ttgttggtgc tttgtattct aaaggattaa aagacgggat gactacttta
     2401 cctttaaaag gtagaaatgc tgtttatgtg gttcgtgtta acaaaacaat tgcagagcca
     2461 gcaacaaaaa catacgaaac agaaaaacaa caattggttg gtcaagcacg ttcttcaatg
     2521 caaaacgatg caattacagg attgcgtgaa aaagtaaatg tagtggataa cagaagattc
     2581 ttaaaaatcg gaattcgcca ttaatttttt ctaagaaatc attttttaaa cgaccttggg
     2641 tttccaaggt cgttttttat ttaacacatt tcattgataa tctttagcaa ttgacattgt
     2701 ataataaaaa attatttcta ttttagtgca ttaatactaa atttaagtta tgaaaaaatt
     2761 aatactttct ttatttattg caggaggggt aggctttggt ttcagtcaaa tgactgtaac
     2821 aggagtatca cctgcaagca tcgtcggcaa ctatacattt agctatggtg agactactag
     2881 cggttggggc tctacgatga acttttctac accaggtgtt catgtacaag gttatttagc
     2941 acttgttgaa gatggtacta caggtacaaa tgcccaagga catccaattt cacaagaagg
     3001 atgttcacct ttaacaaacg gctcgtctgt tacaggtaaa attgcggttg tttacagaaa
     3061 cacatgtgag tttggtgaca aagctaaaaa cgcacaagat gctggtgcaa ttgcagtcat
     3121 tgttgttaac cgcgataacg aagtaattgc tatggggggt ggtacacaag gaccgaacgt
     3181 tacaattcca gttgtaatgt tgacaagtct tgatggtgct ctt
//
LOCUS       DAOQVQ010000264         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34490_length_3223_cov_2.879419, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000264 DAOQVQ010000000
VERSION     DAOQVQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34490_length_3223_cov_2.879419"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKN22_08045"
     CDS             complement(<1..293)
                     /locus_tag="PKN22_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42696.1"
                     /translation="MEEVISSKNKLWTLEQIMSSIDDLDTEENITCVIFTHMIPFQIK
                     RKRNRHFVFCNGKNLEKIMGLESIFPFVIIENMKAFTEDASHAIKDFLLAPNNL"
     gene            complement(293..1966)
                     /locus_tag="PKN22_08050"
     CDS             complement(293..1966)
                     /locus_tag="PKN22_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42697.1"
                     /translation="MASRNCRVAEKSKNLIYASRKFTDLARETAIKIQLNKSALVKRK
                     IDNPVDDMQVRSEHFEKYFDNVADYALDFFGIHFWQKQQEIATALRDKNFVCVRSAHS
                     TGKSYLLGILINFYFDTVYPLVGIGTAPTKALVSSVMFSYARQFRNTNLQILGDYFTG
                     PVMPKLMTTEGHYFEGIVTSDPTSIQGRHGPNVVILLDEAVGITSEMFESLESLMVGD
                     YVKVLAIYNPTDPSSYVAQLEKSAGWHTITMSAYDHPNIWTGVERLAQGRKATEDLPF
                     PGAINLTRFEQLLKQWSFRINKSDYNPSRDIILPTSLLHNELEYYRPGPIASARLLGR
                     WSEVSMNAIFTDYTVDQSIYNILPTTFDEPVTIGVDVARFGSDFSAFCVRHGGKILEL
                     FEVNGLSTVAVTGKVIDLCKKYSERFEIAAQTIDIAVDVIGVGSGVYDNLVDAGYNAH
                     EINVSERAWNAESYGNLRSELWFEVHNLFLTGQVSLGNIASTTIFDLKKQLLAPLFTY
                     DKLGRRQVEPKDDTKKRLDRSPDLADAIMLAFAVNTEFTSGITANRDEG"
     gene            complement(1956..2642)
                     /locus_tag="PKN22_08055"
     CDS             complement(1956..2642)
                     /locus_tag="PKN22_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42698.1"
                     /translation="MTTDLLNLMGDGKEYFDKIDFNELMVDIEIDKIFERSLQEDTLT
                     VEEIMESAPWKQLEGESELQYDTFKYYINLNIDEWEAVNVLRFIDIDEANAVEWCDEF
                     QWKERRLAYLKYQEWLRRKKSELKHTEDIEEFRDSQAELLKSAGSATLNLISKLALRI
                     EDLDPNEIKATDIPKFVSAISSFLDMTADAQARFLSVNELLALYENDLDADVIRQHVL
                     LSEQGKVNGK"
     gene            2744..3106
                     /locus_tag="PKN22_08060"
     CDS             2744..3106
                     /locus_tag="PKN22_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42699.1"
                     /translation="MVKEEVNRFLKYTNKFKKINPELLEFDGIKYVFIVENDNMNDET
                     EVEILIRQSLIEDFHFIIIMKSIPSEPIDNTLLWVINQINDEMSIIIRVLYFDEVTEN
                     YVLLKTENVDKKVKIWQN"
BASE COUNT         1106 a          573 c          441 g         1103 t
ORIGIN      
        1 aggttgtttg gtgctaataa aaaatctttt atagcatgag aagcatcttc tgtaaacgct
       61 ttcatatttt caataatgac aaatgggaat atagactcta atcccataat cttctcaagg
      121 ttttttccat tacagaaaac aaaatgtcta tttctttttc ttttgatttg aaatggaatc
      181 atatgtgtaa aaataacaca tgttatattt tcttctgtat ctaaatcatc aatagaagac
      241 ataatttgct ctaatgtcca taatttattt tttgaagata ttacttcttc cattatccct
      301 catctctatt cgcagttatt ccgcttgtaa attctgtatt aacagcaaat gctaacataa
      361 ttgcatccgc taagtctgga cttctatcta atcttttctt tgtatcatct tttggttcaa
      421 cttgtctacg ccctaattta tcataagtaa ataatggtgc aagaagttgt ttttttaaat
      481 cgaatattgt tgttgaagca atattaccta aagagacttg accagttaag aaaaggttat
      541 gaacttcaaa ccataattca cttcttaaat taccataact ttcagcgttc catgctcgtt
      601 cagaaacatt aatttcatgg gcattataac cagcatcaac taagttatca taaacaccag
      661 atccaacacc aatcacatca actgcaatat caattgtctg tgctgcaatt tcaaagcgtt
      721 cactatactt cttacataga tcaattacct tgcctgttac agcaacagtg cttaatccat
      781 ttacttcaaa taattcaagt attttacccc catgtctaac acaaaatgct gaaaaatctg
      841 aaccaaaacg agcaacgtca acaccaatcg taacaggttc gtcaaatgtt gttggtaaga
      901 tgttgtagat tgattggtca accgtataat cagtaaaaat agcattcata cttacttctg
      961 accatctccc aagtaaacgt gcagaagcga ttggaccagg gcgataatat tcaagttcat
     1021 tatgtaataa tgatgttggt aaaataatat cccttgatgg attataatca gacttattaa
     1081 ttctaaatga ccactgcttt aacaattgtt caaaacgagt taagttgata gcaccaggaa
     1141 aaggtaaatc ttctgttgct tttcttcctt gcgccaatct ttctacacca gtccaaatat
     1201 taggatggtc atatgcactc attgtaatgg tatgccaacc agcagacttt tcaagttgtg
     1261 caacataaga agaaggatct gttgggttgt aaattgctaa tactttaaca taatcaccaa
     1321 ccattaaact ttccaacgat tcaaacattt cagatgtgat accaacagct tcatctaata
     1381 agataacaac attaggacca tgacgacctt gaatgcttgt tgggtctgat gttacaattc
     1441 cttcaaaata atgaccttcc gttgtcataa gtttaggcat tacaggacca gtaaagtaat
     1501 caccaagaat ttgtaaattt gtatttctaa attgtcttgc gtaagaaaac atgaccgaac
     1561 taacaagagc tttagttggt gctgttccta ttccaaccaa agggtaaacg gtatcaaaat
     1621 agaaattaat caaaatacca agaagataac tttttcctgt gctgtgagca gaacgaacac
     1681 atacaaaatt tttatctctt aatgctgttg caatctcttg ctgcttttgc caaaagtgaa
     1741 tcccaaaaaa gtctaaagca taatcagcaa cattatcaaa atacttttca aaatgttctg
     1801 aacgaacttg catatcatca acagggttat ctatttttct tttaactaat gcgctcttat
     1861 ttaactgaat cttgattgct gtctctcttg caagatcagt gaattttctt gaagcatata
     1921 ttaaattttt tgatttttct gctactctac agtttctact tgccattcac tttaccttgt
     1981 tctgataaga gaacatgctg tctaattaca tcagcatcta aatcattttc atataacgct
     2041 aaaagttcat ttacagataa aaatctagcc tgtgcatcag cagtcatatc aagaaatgaa
     2101 gaaatagctg aaacaaattt tggaatatcg gttgctttga tttcattagg atcaagatct
     2161 tcaattctta aagcaagttt ggaaataaga ttaagtgttg cactaccagc acttttaaga
     2221 agctcagctt gactatctct aaattcttca atatcttctg tatgtttaag ttctgatttc
     2281 tttcttctca accattcttg atatttaaga tatgctaatc gtctttcttt ccattgaaac
     2341 tcatcacacc attcaacagc attggcttca tcaatatcaa taaatcttaa tacatttact
     2401 gcttcccatt catcaatatt aagattgatg tagtacttaa atgtatcgta ttgtagttca
     2461 ctttcccctt ctaattgttt ccaaggagca gattccatta tctcttcaac agttaatgta
     2521 tcctcttgca aactacgctc aaagattttg tcaatctcaa tatcgaccat taattcatta
     2581 aaatctatct tatcaaaata ttctttacca tcacccatta agtttaataa gtcagttgtc
     2641 atatttgcct ttcttttacc atgtcaattc acaatttgac atgtaatata attatggtat
     2701 aattcaatta tcaaatcaat aaacagacaa tagaaagagt attatggtta aagaagaagt
     2761 aaatagattt cttaaatata ctaataagtt taagaagatt aatcctgaat tattagaatt
     2821 tgatggtatt aaatatgtat tcattgttga aaatgataat atgaatgatg aaacagaagt
     2881 cgaaatttta attagacagt cattaattga agatttccac ttcatcatta taatgaaaag
     2941 tattccatca gaaccaattg ataatacatt gttatgggtt attaatcaaa tcaatgatga
     3001 aatgtcaatt atcattagag tattatactt tgatgaagta acagaaaatt acgtcttact
     3061 gaaaacagaa aatgttgata agaaagtgaa aatatggcaa aattaactcc aattgaagca
     3121 gcaaatgtga agaaccttca attgcgtcat ccacatacag taaatgtacg ttggaattat
     3181 tttcctcata caggaaataa gatgcaactg tatactaaac aaa
//
LOCUS       DAOQVQ010000265         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34535_length_3220_cov_4.017378, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000265 DAOQVQ010000000
VERSION     DAOQVQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34535_length_3220_cov_4.017378"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /gene="miaB"
                     /locus_tag="PKN22_08065"
     CDS             complement(<1..1399)
                     /gene="miaB"
                     /locus_tag="PKN22_08065"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNS42700.1"
                     /translation="MGSSINIRESRIKVIDEARQGEATLGVETPENPNGKKLFIESYG
                     CQMNFSDSEVVASIMTKNGYSTTKELDNADVVLINTCAIRDNAETRVRNRLSEFKKRK
                     SDNPNLVVGILGCMAERLKTNLLEEEQLVDLIAGPDAYRDLPNLVEEVGSGQRAVNVL
                     LSREETYADISPVRLDIGGINAFVTIMRGCDNMCSFCVVPFTRGRERSRDPQTIVSEC
                     VDLFNKGYREVTLLGQNVNSYRWNMTSKGEVKDETIATTNFAQLLEMVAKVSPDLRVR
                     YSTSHPKDMTDEVLEIMAKYENICEYIHLPVQSGNTQVLARMNRNYTREWYMNRINAI
                     RKYMPNCAISTDIITGFCGETDEEHNDTISLMKEVRYEYAYMFKYSERPKTLAERKFE
                     DDVPEEVKSKRLNEIIDLQQADSLYWNKKQIGTVTKVLVEGTSKRSEDEWCGRDGRNS
                     MVIFPKGNSEKGQYIM"
     gene            1614..2123
                     /locus_tag="PKN22_08070"
     CDS             1614..2123
                     /locus_tag="PKN22_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009746623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNS42701.1"
                     /translation="MKKVAYSLTVALSLIVLASCGGKQEPAAETPATTTEETATTPAE
                     PITLELNAGDDMKYDKTEFTVKAGQKVTLNFHHTGKMAKEAMGHNFVLLQEGVDVAAF
                     GQAAAGAAATGYIPESMKGDIIAHTDLIGGGESTTLEFTAPTTAGNYAFICSFPGHYG
                     MMKGIFIVE"
     gene            2255..>3220
                     /locus_tag="PKN22_08075"
     CDS             2255..>3220
                     /locus_tag="PKN22_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS42702.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLQTTGTISDREMQDQALDNM
                     DLERERGITIKSHAIQMNYKFEGENYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ASQGIEAQTISNLYLAIEHDLEIIPILNKMDLPGAMPEEVTDQIVDLLGCDPSEVIPA
                     SGKTGLGVDKILEAVIKRIPAPKGDENAPLQAMIFDSVFNPFRGIIAYYRIKNGVLKK
                     GQQVKFFNTGKEYIADEIGTLKMDLMPKNEVKAGDVGYIISGIKQANEVKVGDTITSK
                     TNPCSEAIKGFEDVKPMVFAGIYPVDTEDYEELRYSMEKLQLNDSS"
BASE COUNT         1033 a          602 c          569 g         1016 t
ORIGIN      
        1 ccatgatgta ttggcctttt tcagaatttc ctttcgggaa aatcaccatc gaatttcttc
       61 catcgcgacc acaccattcg tcttctgaac gtttagaggt accttcaacc aatacttttg
      121 ttactgtgcc aatttgtttt ttattccaat acaatgaatc cgcttgttgc aaatcgatga
      181 tttcgttcaa acgtttgctt ttcacctctt ctggaacatc gtcttcaaac ttacgttcag
      241 ccaacgtttt agggcgttca gaatatttaa acatataagc atattcataa cgcacttctt
      301 tcattaagct aatcgtatcg ttgtgttctt catccgtttc gccacaaaat ccagtgatga
      361 tgtcggtaga aatagcacaa tttggcatgt attttcgaat ggcgttaatt ctgttcatgt
      421 accactcgcg tgtgtaattt ctgttcatac gagccaatac ttgcgtgttt ccagactgaa
      481 caggaaggtg aatgtattcg caaatgtttt catattttgc catgatttcc aatacttcat
      541 cggtcatgtc ttttggatgc gaggttgaat aacgcacacg taaatccggt gatacttttg
      601 caaccatttc cagcaattga gcaaaatttg ttgttgcaat tgtttcgtct ttcacttcac
      661 cttttgaggt catgttccaa cgatagctgt taacattttg tcccaaaaga gtaacttctc
      721 tatatccttt attgaataaa tcaacgcatt cactaacaat ggtttgcggg tctcttgagc
      781 gttctctacc tctggtaaat ggaactacac aaaacgaaca catgttgtcg caaccacgca
      841 taatggttac aaatgcatta attccgccaa tatctaagcg aacgggtgaa atatcagcat
      901 acgtttcctc acgagaaagc aacacgttta cagctcgttg tcccgaacca acctcttcca
      961 ccaaatttgg taaatcacga taagcatctg gtccagcaat taaatccacc aattgctctt
     1021 cttccaataa gttggttttc aaacgctcag ccatacagcc aaggatacca acaaccaaat
     1081 taggattatc agattttcgt tttttaaatt cagacaagcg gtttctaaca cgtgtttcag
     1141 cattgtcacg aatagcgcaa gtattgatta ataccacatc agcattatcc aattcttttg
     1201 tagtagaata cccatttttg gtcataattg aagccacaac ttcactgtcc gaaaagttca
     1261 tttggcatcc ataactttca ataaaaagct ttttaccatt tggattttca ggtgtttcaa
     1321 ctcctaaagt tgcttctcct tgacgtgctt cgtcaattac tttaattcta ctctctctaa
     1381 tatttatgct tgaacccatt ctttacttta aaatagagtg caaagatagc aataaataaa
     1441 attctgccaa attgtcacaa tgatttaact atttttaaaa taagttgtcc aacagccatt
     1501 gttttcaagt ggtttataaa taaataagat atttttgtat tattttatta tttagattta
     1561 ttttaaataa taattaattc gtaaatttgc tgaaacttta aaatttttaa aagatgaaaa
     1621 aagtagcata ttctttaaca gtggcattgt cactaatcgt tttggcttct tgtggtggaa
     1681 aacaagaacc agcagcagaa actccagcaa caacaactga agaaacagca acaactccag
     1741 cagaaccaat tacattggaa ttaaatgctg gtgatgacat gaaatacgac aagacagaat
     1801 ttaccgtaaa agctggtcaa aaagtaacat tgaacttcca ccatacagga aaaatggcaa
     1861 aagaagcaat gggacacaat tttgtattgt tgcaagaagg tgtagatgtt gcagcatttg
     1921 gtcaagcggc agctggtgca gcagcaacag gttacattcc agaatctatg aaaggagata
     1981 tcattgcaca tactgattta attggtggtg gagaatctac aactttagaa tttacagcac
     2041 caacaacagc aggaaattat gcatttattt gttctttccc tggtcactat ggaatgatga
     2101 aaggaatttt cattgttgaa taagacacaa aaataaattt ttgaaaagac cgtaattgaa
     2161 agttgcggtc tttttttata gtcataatcg agcctatcta tacaaatatt aaaattttag
     2221 cttacttttg catcccatag ccagattgta gtttatgaag aacattcgaa atttttgtat
     2281 tatcgcccac attgaccacg gtaaaagtac cttggctgat agattgttgc aaaccaccgg
     2341 tacaatttct gaccgtgaaa tgcaagatca agcattagat aatatggacc ttgaaagaga
     2401 acgtggtatt accatcaaat ctcacgcaat tcaaatgaac tacaaatttg aaggtgaaaa
     2461 ttacgtgttg aacctaattg acactccggg acacgtggat ttttcttacg aagtatctcg
     2521 ttccattgct gcatgtgaag gagctttgtt gattgtggat gcatcgcaag ggattgaagc
     2581 acaaaccatt tccaacttat atttggcaat agaacacgat ttggaaatta ttcctatcct
     2641 caataaaatg gacttaccag gagcaatgcc agaggaagtt actgatcaaa ttgttgacct
     2701 tttggggtgc gatccaagcg aagttattcc agcgtctggt aaaacaggtt tgggtgttga
     2761 taaaatttta gaagcagtta ttaaacgtat tcctgcacca aaaggcgatg aaaacgcacc
     2821 gttgcaagcc atgatttttg actctgtatt taatccattt agaggaatta ttgcttatta
     2881 ccgtattaaa aatggggtac ttaaaaaagg acaacaagtt aaattcttta atacaggaaa
     2941 agaatatatt gctgatgaaa tcggtacgtt gaaaatggat ttaatgccaa aaaacgaagt
     3001 aaaagcgggt gacgttggtt acattatttc gggtattaag caagccaatg aggtaaaagt
     3061 gggggatacc atcacttcca aaacaaatcc ttgttcagaa gcaattaaag gttttgagga
     3121 tgtaaaacca atggtatttg ctggtattta cccagttgat acagaagatt acgaagaatt
     3181 gcgttattca atggaaaaat tgcaattaaa cgactcatcc
//
LOCUS       DAOQVQ010000266         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34566_length_3218_cov_4.127727, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000266 DAOQVQ010000000
VERSION     DAOQVQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34566_length_3218_cov_4.127727"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKN22_08080"
     CDS             complement(<1..198)
                     /locus_tag="PKN22_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS42703.1"
                     /translation="MNNLFTVVVTLLFLNTIGYSQKIKGSDTVLPLTEKAAQAYMKKN
                     SSKKIAVTGGGTGVGIEALIEG"
     gene            complement(287..1558)
                     /locus_tag="PKN22_08085"
     CDS             complement(287..1558)
                     /locus_tag="PKN22_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42704.1"
                     /translation="MHSLIIYMTFRILFIIILLSFVNNSYSQKKDSLVSDTNRIHFEQ
                     KKSFFDHFKLTGYAQIQFQHGEENASLSVGSKNSSNAPFNRIGIRRGRIKLQAERGIA
                     LGVFQIDITEEGVGIKDLYVDITDPWLKSNSIRVGVFNRNFGNEIVFSSSRRLNPERS
                     KVTTTLFPQERDMGAMLTLQAPKKSRWNPLKLELAVVAGNGDKLEIDNKKDFIGSFTY
                     TKTFKNNFNFGTGFSYYYGKTHQGSANVYRMQGKSMVVTTDSSFIGQYAKREYFGVEA
                     LFSFKSRIGSTQIDCEYIFGTQPGAINNSKSPNFSSLPTTDTYIRKFQGGYIYLYQSL
                     GKLPLYLVLKYDFYDPNIQVKKNDIGLNGTSKGDVLFQRIGIGLTWDATSNLRIFAYY
                     DFINNETSANLLNYNKDLKDNVFTLRLQYRF"
     gene            1684..2415
                     /locus_tag="PKN22_08090"
     CDS             1684..2415
                     /locus_tag="PKN22_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648145.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNS42705.1"
                     /translation="MKKQLKTIILSLTVIIAALVLGNAYKFKYKQQDRVFVTGLGTED
                     FTSDLIVWQANFSKKGSDLKTISSQLSADREKIKNYLMSKGISDKEIVFAAVNINKEF
                     TSNYDANGNYMGSTFAGYSLTQNLRIESKEVDKIERISREVSELIDQNIELTSYDPEY
                     YYTKLDELKLRMIENATKDAKERAEIIAKTAGSKLSDLKNASMGVIQITAKNSSEDYS
                     WGGSFNTTSKLKTASITIRLEYSLK"
     gene            2468..>3218
                     /locus_tag="PKN22_08095"
     CDS             2468..>3218
                     /locus_tag="PKN22_08095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS42706.1"
                     /translation="MDISVVIPLFNEDESLPELHAWIKRVMDAHQFSYEVVFVDDGSK
                     DKSWQVIEQLAQENKEVRGIKFQRNYGKSAALHTGFEAVIGKVVITMDADLQDSPDEI
                     PELYRMITEQDFDVVSGWKKKRYDPISKTIPTKLYNWTARRLSGIYLHDFNCGLKAYK
                     LAVVKSIEIYGDMHRYIPVLAKFAGFSNIGEKVVQHQARKYGSTKFGLNRFVHGPLDL
                     ISVVFMGKFAKKPMHFFGAVGIVMFLIGFGFS"
BASE COUNT         1081 a          531 c          552 g         1054 t
ORIGIN      
        1 accttcaatt aatgcttcaa ttccaacacc tgttccacca cctgtaacag caattttctt
       61 agaactgttt tttttcatgt aggcctgcgc cgctttttca gttagaggta aaactgtgtc
      121 agaccccttg attttttggc tataaccaat tgtgtttaaa aaaagtagag ttacaactac
      181 tgtaaataaa ttattcatat tgttttaatt tacagcaaaa ttagatggat gatattacgt
      241 cgggttttag ataaaattac aaaacggtta agaaatagta ttgatgttaa aatctatatt
      301 gcaaccttaa agtaaaaaca ttgtctttta agtctttgtt gtaattcaat aagtttgccg
      361 atgtttcatt atttatgaaa tcataatatg caaagattct taagttacta gttgcatccc
      421 aagttaaacc tattccaatg cgctgaaata atacatctcc tttacttgtt ccatttaagc
      481 caatatcgtt cttttttacc tgaatgtttg gatcgtaaaa atcatatttt agtaccaagt
      541 agagtggcag tttccctaaa ctttgataca aataaatgta gccaccttga aattttctta
      601 tgtaagtatc agtagtgggt aacgaactaa aattggggct tttcgaatta tttatagcac
      661 ctggctgtgt cccaaagata tactcacaat caatttgagt tgaacctatt ctacttttaa
      721 aagaaaataa tgcttctaca ccaaaatatt ctctttttgc gtattgtccg ataaaagatg
      781 aatcagttgt gacaaccatt gattttcctt gcattcgata cacatttgca ctcccttgat
      841 gtgtttttcc ataatagtag gagaaacccg ttccgaagtt gaaattattt ttgaatgttt
      901 tggtataggt gaaacttcct ataaaatctt ttttgttgtc aatttcaagt ttatcgccat
      961 ttcctgcaac aactgcaagc tctaatttca atggattcca acgagatttt ttgggagctt
     1021 gtagagttaa cattgcaccc atatcacgtt cttgaggaaa taatgttgta gtaaccttag
     1081 agcgctcagg gtttaaacgg cgagaggaag aaaatacaat ttcattccca aaatttctat
     1141 taaaaacacc aactcttatg gagttagatt tcaaccaagg atcagttata tctacatata
     1201 aatctttgat tccaacacct tcttccgtaa tatcaatttg aaaaacccca agtgcaatac
     1261 ctcgttcagc ttgcagttta attcgtcctc tacgaatacc tattctatta aatggagcat
     1321 tagaagaatt tttactccct acagataagg atgcattctc ttcgccatgt tgaaattgaa
     1381 tttgtgcata tcctgtgagt ttaaagtggt caaaaaacga ttttttttgc tcaaagtgaa
     1441 tacgatttgt atcacttacc aaagagtctt tcttttgaga ataactgttg ttaacgaaag
     1501 aaagtaaaat gataataaaa agtattctaa atgtcatata tattattaaa gagtgcaaaa
     1561 aaatctattt attgttgcaa gcaagtatcg aaaatgttac gattttgtta caattatttt
     1621 ttacactgta aaaactgttt ttataaaata aaatcctact tttactgaaa tcaataattt
     1681 actatgaaaa aacagctaaa aacaatcatt ttatcactaa ctgtcatcat tgcagcgttg
     1741 gtgttgggca atgcttataa attcaaatac aaacaacaag atagagtgtt tgtaaccgga
     1801 ttgggtaccg aagattttac gtcagattta atagtgtggc aagctaactt ttctaaaaaa
     1861 ggaagtgatt tgaaaacaat ttcaagtcag ttgagtgccg atagagagaa aattaaaaac
     1921 tacctgatgt caaaaggaat ttcggacaaa gaaattgtgt ttgcagccgt taacatcaat
     1981 aaagaattta ctagtaatta cgatgcaaat ggtaattata tgggatctac ttttgcgggc
     2041 tattccctca ctcaaaactt gcgaattgaa tccaaagaag tggataaaat tgaacgaatt
     2101 tcgcgcgaag tgtccgaatt aattgatcaa aatatcgaat taacttctta tgatccagaa
     2161 tattattaca ccaagttgga tgaattgaaa ttacgcatga ttgaaaatgc aaccaaagac
     2221 gccaaagaac gagcagaaat tattgcaaaa acagcaggta gcaaattgtc agacttaaaa
     2281 aatgcatcaa tgggcgtgat tcaaattaca gcaaaaaatt catcggaaga ttacagctgg
     2341 ggaggttcgt ttaacacaac atcaaaacta aaaacagctt ccattaccat tcgtttggaa
     2401 tattcactca agtaattcag caattctttt atatttgtac tcttaaaatt ataaagtgga
     2461 agcaaagttg gatatttctg ttgtaatacc tttgtttaac gaagatgaat cgttgcccga
     2521 attgcatgcg tggattaaac gcgtgatgga tgcacaccaa ttctcctatg aagttgtttt
     2581 tgttgacgat gggagtaagg ataaatcgtg gcaagtcatt gagcaattag cacaagaaaa
     2641 taaagaagtt agaggcatta aatttcaacg aaattatggc aagtcagctg ctctccacac
     2701 tggatttgaa gctgtaatag gcaaagttgt cattacaatg gatgccgatt tgcaagattc
     2761 tcccgatgaa attcctgaat tgtatagaat gattaccgaa caggattttg atgttgtttc
     2821 aggatggaag aaaaaacgct acgatccaat ttctaaaacc attccaacta aattgtataa
     2881 ctggacagcg cgccgtttgt cgggaattta tttgcacgat tttaattgcg gactaaaagc
     2941 ctataaactt gctgttgtta aaagcattga aatttatggc gatatgcacc gatacattcc
     3001 agtattggca aaatttgcag gtttttcaaa cattggcgaa aaagtggtgc aacaccaagc
     3061 acgaaaatac ggatccacta aatttggtct taaccgtttt gtgcacggac cattggattt
     3121 aatctccgta gtgtttatgg gtaaatttgc taaaaaaccc atgcatttct ttggagcggt
     3181 tggaattgta atgttcctca tcggatttgg cttttctt
//
LOCUS       DAOQVQ010000267         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34831_length_3202_cov_3.475056, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000267 DAOQVQ010000000
VERSION     DAOQVQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34831_length_3202_cov_3.475056"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKN22_08100"
     CDS             <1..201
                     /locus_tag="PKN22_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42707.1"
                     /translation="MLIPSVQVPIFGIYEWRKGASDFKWFDSNYYPMLVKVKWTYLFE
                     KKVKGCAPARVNDQDKNTMKNQ"
     gene            201..551
                     /locus_tag="PKN22_08105"
     CDS             201..551
                     /locus_tag="PKN22_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNS42708.1"
                     /translation="MKRIYHLKTCSTNQRILKELNTDGFELINIKENNIDEQTLDWLA
                     QKVGSYEALFSKKSLKYKTLGLNEQQLSESDYKRWMLEEYTFLKRPFIINGDAVFIGN
                     AKETVAMAQQSLNK"
     gene            578..1528
                     /locus_tag="PKN22_08110"
     CDS             578..1528
                     /locus_tag="PKN22_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS42709.1"
                     /translation="MNNKIKAHIALFSVNAFYGANNIIAKDVMPTYLTPNVFIAFRVL
                     GATFLFWLISLLSKREKVERKDLILLAICGIFGVAINQLCFFHGLNRSSAINSGIIMT
                     INPIAVAIMAYFILKESLNATKIIGIFIGAIGASLLAIQSNSTGGGTLLGDVLLLINA
                     LSYAVYLILVKPLMSKYSALTVTTYVFSFGALYVFIFPQTLLYFASIDFAAIPWDVWL
                     RIAYVIVCVTFLTYLLTMYAMKFVSTSVTSSYIYLQPILVIVFAYLFYYLGISGDYTK
                     SITTTKIALMFLVFFGVYLVIRSEKISTNFQQIKGKFKRN"
     gene            complement(1531..>3202)
                     /locus_tag="PKN22_08115"
     CDS             complement(1531..>3202)
                     /locus_tag="PKN22_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42710.1"
                     /translation="TTNLEKATVHGGATNSATGASIAGSLDLVRKRAGFGAQQFKGAV
                     FTGIETNNWQKIGGTSLSYSRPKFYTDFDFTFRDAENYKAGKRKEVLYSQYTKFNTST
                     NLGVKLGEHRQLEGTFIYDHALNIGYPALPMDVSTAKAFIGAIEYSQHHISEKLHLIK
                     AKVYYNDVTHIMDDSKRPIVPIRMDMPGWSKTAGFYTLLEGNANDFLAWKFNISGHHN
                     QSLAEMTMYPNNPNEKEMFMLTWPGVHTNYGDLYGDIAILRYDDIKIAINAGIGVHHN
                     RIYDDFGYKSLAIFYPQFNPDKVRVLPRTAINFTYKKYNKAFWEYTAGINYGERAPSV
                     SEGYGFYLFNSFDKFDYIGNPLLKNEKSASFATSAAFIHSKLTVRLSANYFFIKDYII
                     GRQDSILSAMTIGANGVKVYEQLNYAHLFNGNLNIEYRFVDHWKWNNKLSIRRGVGEQ
                     SINLPMIQPFTYSSALECNYGGFSSQLEVAGAAKQYFASKEFGESAVPAYAVLNFSAA
                     YKLEIKRHTLAFKAGVENILDSYYTTFADWNRIPRMGRNFYLNIVWGF"
BASE COUNT          947 a          605 c          581 g         1069 t
ORIGIN      
        1 atgctcattc catcggtgca agttccaatt tttggaattt atgaatggcg caaaggtgct
       61 tcggatttca aatggtttga ttccaactat tacccaatgt tggttaaagt aaaatggacg
      121 tatttgtttg agaaaaaagt taaaggttgc gcccctgcac gtgtcaacga tcaagataaa
      181 aacaccatga aaaaccaata atgaaacgca tttatcatct caaaacgtgt tctaccaatc
      241 aacgcattct caaagaattg aataccgatg gttttgaact cattaacatt aaagaaaata
      301 acattgatga gcaaacattg gattggttgg ctcaaaaagt aggttcttac gaagctttgt
      361 tttcaaaaaa atccttgaaa tacaaaactt taggactgaa tgagcaacaa ctatccgaat
      421 cggattataa acgttggatg ttggaagaat acactttttt gaaacgccct tttattatca
      481 acggcgatgc agtttttatt ggtaacgcaa aagaaactgt ggcaatggct caacaatcgt
      541 tgaataaata acgatatttg ctgccaaaac agtggggatg aacaacaaaa tcaaagcaca
      601 cattgctctt ttttcggtca atgcatttta tggtgccaac aacatcattg ccaaagatgt
      661 tatgcctact tatcttactc ccaatgtttt tatcgcattt agagtacttg gtgcaacgtt
      721 tcttttttgg ctaatttcat tgctatcaaa acgtgagaaa gttgaacgaa aagacctcat
      781 cttattagca atatgtggaa tttttggtgt tgcaattaac caattgtgct tttttcatgg
      841 attgaaccgt tcttcggcca tcaattcggg aattattatg accattaatc caattgctgt
      901 ggctatcatg gcttatttca ttttaaaaga atctctgaac gccacaaaaa ttattggaat
      961 ttttattgga gccattggag ccagtttgtt agccattcaa tccaactcaa ctggtggtgg
     1021 cactttgcta ggcgatgttt tattgctcat caatgctttg agttatgccg tttatctcat
     1081 cttagttaaa ccattgatga gtaaatattc ggcattaacg gttacaacct atgttttttc
     1141 gtttggtgca ttgtatgttt ttatttttcc gcaaacgttg ttgtattttg catctattga
     1201 ttttgctgcc attccgtggg atgtgtggtt gcgcattgca tacgttattg tctgtgtaac
     1261 gtttttaacc tatttactca ccatgtatgc catgaaattt gtgagtacat cggtaaccag
     1321 ttcctacatt tatttgcaac cgattttggt catcgttttt gcttatttgt tttattattt
     1381 aggaatttct ggcgattaca caaaatccat cacaactacc aaaatcgcac ttatgttttt
     1441 ggtgtttttt ggcgtttatt tggttattcg ttccgagaaa atcagcacca atttccaaca
     1501 aataaagggg aaatttaaaa gaaattaagc tcaaaatccc cacacaatat tcaaatagaa
     1561 gttacgtccc atacggggaa tgcgattcca atcggcaaag gtggtatagt aactatccaa
     1621 tatattctca acacctgctt tgaaagccaa tgtgtgtctt tttatctcca atttataggc
     1681 tgccgaaaaa ttaagaacgg cgtatgctgg cactgctgat tcaccaaact ctttactggc
     1741 aaaatattgt tttgcagctc ctgcaacttc caactgacta ctaaaacctc cataattgca
     1801 ttctaatgca gaagaatagg taaaaggttg aatcattggt aaattaatgg attgctcacc
     1861 cactcctcta cggatactta atttattatt ccatttccag tggtccacaa aacggtattc
     1921 aatgtttaga tttccgttaa acaaatgagc ataatttaac tgttcatata ctttcacccc
     1981 gtttgcacca atggtcatgg cacttaaaat actatcttgt cgcccaatga tgtaatcctt
     2041 gataaaaaag taattagccg acaaacgcac agttagtttt gaatgaataa atgctgccga
     2101 agttgcaaaa gaagctgatt tttcgttttt aaggagtgga ttgccaatgt aatcaaactt
     2161 atcaaaacta ttgaacaaat aaaatccata tccttcggaa acgcttggag cacgttcgcc
     2221 ataatttata ccagcggtgt attcccaaaa tgctttgttg tattttttgt aagtgaaatt
     2281 aattgcagta cgaggtaaaa ctcgcacttt atcggggtta aattgcgggt aaaaaattgc
     2341 aaggcttttg tagccaaaat catcgtaaat gcggttgtgg tgaacgccaa tacctgcgtt
     2401 gattgctatc ttaatatcgt catatcgcaa tattgcgata tcaccataaa ggtcaccata
     2461 attggtgtgc acaccaggcc aagtcaacat aaacatttcc ttttcattgg gattatttgg
     2521 atacatggtc atctctgcca acgattggtt gtggtgtcca cttatattaa atttccacgc
     2581 taaaaagtcg tttgcattcc cttccaacaa ggtataaaat ccagcggttt tactccatcc
     2641 aggcatatcc attcgaatag gtacaattgg tcgtttggaa tcatccatga tatgagttac
     2701 atcattgtag tacacttttg cttttatcaa atgtaatttt tcagaaatgt ggtgttgact
     2761 gtattcgatg gctccaataa atgcttttgc ggtggaaacg tccatgggca aggccggata
     2821 gccaatgttg agggcatgat cgtaaataaa tgttccttcc aactgacggt gttctcccaa
     2881 ttttacgcct aaattggtcg atgtgttgaa ttttgtgtat tgagaataca gcacttcttt
     2941 tcgttttcct gctttgtaat tttcagcatc tcgaaacgta aaatcaaagt cggtgtagaa
     3001 tttgggacgc gaataggaca aacttgtacc acctattttt tgccaattat tggtttcaat
     3061 tccagtaaaa acagctcctt taaattgttg tgcgccaaaa ccagctcttt ttcgtaccaa
     3121 gtctaaactt ccagcaatgg atgcacctgt tgcagaattg gttgctcctc catgcaccgt
     3181 tgctttttcc aaatttgttg tt
//
LOCUS       DAOQVQ010000268         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_34999_length_3192_cov_4.066624, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000268 DAOQVQ010000000
VERSION     DAOQVQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34999_length_3192_cov_4.066624"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="atpH"
                     /locus_tag="PKN22_08120"
     CDS             complement(<1..478)
                     /gene="atpH"
                     /locus_tag="PKN22_08120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNS42711.1"
                     /translation="MKGTKSASRYAKALLELAVEGNQVDAVLNDMKYLASVANSSRDF
                     QLLLQSPIINSDKKVAIFNELFGSFQKLSTAFIELITKKGREALIADIANSFEDQVNL
                     YKGITPATITSAVPLHEDAKKKILEKIKQITSGNVEITEKIDASLIGGFIVRAGDKQ"
     gene            complement(485..979)
                     /locus_tag="PKN22_08125"
     CDS             complement(485..979)
                     /locus_tag="PKN22_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNS42712.1"
                     /translation="MELITPDLGLLFWTGLVFVLLLFILTKFAWKPILNMVNTREQKI
                     TEALELAEKTKAEMQQLAAQNEALLKEARAERDTMIKEAKATADKMVDDAKSKAKEEA
                     EKVLEGARASINAEKAAAVAELKAQMAAFSIEIAEKVVRENLSSDEKQKTLADKLVEE
                     INLN"
     gene            complement(1052..1258)
                     /gene="atpE"
                     /locus_tag="PKN22_08130"
     CDS             complement(1052..1258)
                     /gene="atpE"
                     /locus_tag="PKN22_08130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNS42713.1"
                     /translation="MTGTVAAIGAGLAVLGAGLGIGRIGGSAMDAIARQPEASGKIQT
                     AMIIAAALIEGVALFGVVVGFLGL"
     gene            complement(1294..2409)
                     /gene="atpB"
                     /locus_tag="PKN22_08135"
     CDS             complement(1294..2409)
                     /gene="atpB"
                     /locus_tag="PKN22_08135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNS42714.1"
                     /translation="MIGQNTIKSIVLAVAVLLGCTVSYAETTHKEDELELVHEFNVSE
                     FIMHHIKDAHEIHLWGGAHDGVSIYLPMILLDNGTKVFSSSLLYHGEEKIAVDHATHD
                     SIHYTVGVGAAEGYALYHEKVYKLENGELHFQDGHPHNAKPTSLSITKNVVVLFLVSL
                     IVCWFAFSTAKHYKKNGAVAPKGIAKFIEPLVIFVRDDIAKDNIGEHKYQKYTPYLLT
                     VFFFVLTGNLLGLVPMLAANLTGNITITFFLAVCTLLATVLSGNKNYWQHIFATPGVP
                     KPLLIIMMPIELVGILTKPFALMIRLFANMTAGHVIILAFIGIIFINKNIAWGALSVP
                     MALFISVLEILVAFLQAYLFAMLSALFIGAAVEEAHH"
     gene            complement(2496..2888)
                     /locus_tag="PKN22_08140"
     CDS             complement(2496..2888)
                     /locus_tag="PKN22_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42715.1"
                     /translation="MTKSKYISLIVAATLICLLLFGALHYFKILSLPIKLFGLINVVI
                     PALFLGSIYVVSGDKSKVEVFVQRFMILTTFQLLTNLFLIAAVWYVAKPYLKQFGLQL
                     ISVFILLMAIQSILLIRFSVTQSNKKNN"
     gene            complement(2875..3123)
                     /locus_tag="PKN22_08145"
     CDS             complement(2875..3123)
                     /locus_tag="PKN22_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009746048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNS42716.1"
                     /translation="MSQSKEPASKKNFNTFARFSTVGIQMGVIIGGFAFLGDYLDKKY
                     NNSTPWWTLGLVLFGVAAGLTLVIREAMRFSERQNDKK"
BASE COUNT         1040 a          657 c          447 g         1048 t
ORIGIN      
        1 tttgcttatc acctgctcta acgataaatc caccaattaa tgaagcatca attttttcag
       61 tgatttccac attacctgat gtaatttgtt taattttttc caatatcttt ttcttagcgt
      121 cttcatgcaa aggtacagca gatgtaattg tagctggtgt aattcctttg tataaattca
      181 cttgatcttc gaaagaattt gcaatatctg caatcaatgc ttctctacct tttttggtaa
      241 tcaactcgat aaaagccgta ctcaactttt gaaatgaacc aaataattca ttaaaaattg
      301 ctactttctt atcagaattg ataattggtg attgtaaaag caattgaaag tcacgagaac
      361 tattcgcaac agatgcaaga tacttcatat cattcaaaac tgcatcaact tgattgcctt
      421 caacagccaa ttcaagcaaa gcttttgcat atctcgatgc tgattttgta cctttcatct
      481 tttattaatt caaattaatt tcttcaacta atttatcagc caatgttttt tgtttttcat
      541 cgcttgataa attttcacgc accacttttt cagcaatttc aattgagaat gctgccattt
      601 gtgctttcaa ttctgcaact gctgctgctt tctcagcatt gatagatgca cgagcacctt
      661 ccaatacttt ttcagcttct tctttagctt ttgattttgc atcatcaacc attttatcag
      721 ctgtagcttt tgcttcttta atcattgtgt cgcgttctgc acgtgcttct ttcaacaagg
      781 cctcgttttg agctgccaac tgttgcattt ctgcttttgt tttttcagct aattctaatg
      841 cttctgttat cttttgctca cgtgtgttca ccatattcaa aataggtttc cacgcaaatt
      901 ttgtcaaaat aaacaacaat agtacgaaaa caagtcccgt ccaaaataac aatcctaaat
      961 ccggtgtaat taattccatt ttatttttat ttgctattaa cgtttaaata aatcggtttg
     1021 caaccaaccg ttgcaaaccg acttggcttt cttaaagacc aaggaaacca acaacaacac
     1081 cgaaaagagc taccccttca atcaatgctg ccgcgataat catagccgtt tgaattttac
     1141 ctgaagcttc tggttgacga gcgattgcgt ccattgctga accaccgatt ctaccaatac
     1201 ctaatcctgc tcctaatact gcaagacctg ctccgattgc tgctactgtt cctgtcattt
     1261 tactttattt taagttaaca aattactatt taattagtgg tgcgcttctt ctactgctgc
     1321 cccgataaac aatgctgaaa gcatcgcaaa taagtaagct tgtaaaaacg ctaccaatat
     1381 ctccaatacc gaaataaaca atgccatagg aactgataac gctccccaag caatattttt
     1441 attgataaag ataattccga tgaatgctaa gataattacg tgacctgctg tcatgttagc
     1501 aaacaaacga atcatcaagg caaatggttt tgttaaaata cctaccaact caattggcat
     1561 catgataatt aacaaaggtt ttggcactcc tggtgttgca aaaatgtgtt gccagtaatt
     1621 tttattaccc gacaatactg ttgctaacaa tgtacaaaca gctaaaaaga aagtaatggt
     1681 aatattacct gttaaatttg ctgccaacat tggaaccaac cccaacaagt ttcctgtcaa
     1741 tacgaaaaag aataccgtta ataaatacgg cgtgtatttt tggtatttat gctcaccgat
     1801 attatctttc gcaatatcat cacgcacaaa aattactaaa ggctcaataa atttagcaat
     1861 accttttggt gcaactgctc catttttctt ataatgctta gctgttgaaa acgcaaacca
     1921 acacacaatc aacgaaacca aaaacaatac aactacgttt tttgtaattg acaaacttgt
     1981 tggttttgca ttgtgcggat gcccgtcttg aaaatgcaat tctccgtttt ctaatttata
     2041 tactttttca tgatacaacg catatccttc tgcagctcca acacctactg tatagtgaat
     2101 agaatcgtgt gttgcatgat caacagctat tttctcttca ccgtgataca ataacgacga
     2161 agaaaaaact tttgtaccat tatccaacaa aatcattggc aaataaattg aaacaccatc
     2221 gtgtgctcca ccccacaaat gaatttcgtg cgcatcttta atgtgatgca taatgaattc
     2281 actcacgtta aactcatgca ctaattctaa ctcatcttct ttatgcgttg tttcagcata
     2341 agataccgta caacctagca aaactgctac ggctaatact attgatttta ttgtattttg
     2401 tccaatcatc acaccaaaaa ctttggatat actttaaaaa tttggcgcaa aggtagctaa
     2461 actttcttaa gaattggtat aaataagtaa gttttttaat tatttttttt gtttgattgg
     2521 gtgacactaa atctaatcaa caagattgat tgaattgcca ttaaaagaat aaacacacta
     2581 attaattgaa gtccaaattg ctttaaataa ggttttgcaa cataccaaac tgccgcaatt
     2641 aaaaataaat tggtcagcaa ttgaaaggta gtaagaatca taaaccgttg tacaaatact
     2701 tctaccttgc ttttatcgcc cgaaacaaca taaatacttc ctaaaaacaa tgctggaatc
     2761 acaacattaa tcaacccaaa caattttata ggcaaactaa gtattttaaa ataatgcaaa
     2821 gcaccaaaga gcaacaaaca aattaaagtt gctgcaacta tcaaagaaat gtatttactt
     2881 tttgtcattt tgtcgttcac taaatctcat tgcttcgcga ataaccaaag ttaaacccgc
     2941 tgcaacgcca aataaaacca acccgagggt ccaccatggt gttgaattat tatacttttt
     3001 atccaaataa tcgcctaaaa atgcaaagcc accaatgata acacccattt gtatcccaac
     3061 agttgagaat cgggcaaagg tattaaagtt ttttttggat gctggctctt tgctttgtga
     3121 catgagtgaa attttctata aagatagaaa aaaacactaa atccccataa aatctttcgg
     3181 acattgatac ct
//
LOCUS       DAOQVQ010000269         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_35302_length_3175_cov_3.316026, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000269 DAOQVQ010000000
VERSION     DAOQVQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35302_length_3175_cov_3.316026"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PKN22_08150"
     CDS             complement(<1..121)
                     /locus_tag="PKN22_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNS42717.1"
                     /translation="MKKAIHIEGAPQPIAPYSQAIQSGNFIFVSGQIPVNPFTG"
     gene            184..750
                     /locus_tag="PKN22_08155"
     CDS             184..750
                     /locus_tag="PKN22_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNS42718.1"
                     /translation="MKVLIIDNYDSFTYNLYHYIQKCGVEVVVKRNDELQLAEMDAYD
                     KLVLSPGPGLPSETTLMFDCIEKYANSKPILGVCLGMQGIANYYGGELINQAKVKHGV
                     EALVNIDTTSPLFKNLDEQITVGLYHSWCVDEQTLPSDFKVIGKSAEQVVMAIEHKKL
                     PIYGVQFHPESIMTPNGLQIIANFLAIQ"
     gene            839..1429
                     /locus_tag="PKN22_08160"
     CDS             839..1429
                     /locus_tag="PKN22_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNS42719.1"
                     /translation="MLGLKMPTDPRWLNIAEKNLEEVLIDHAFCEQKAASNAISMIVQ
                     YPHLTDLVEEMTAISIEEMQHFEMVHKELQRRGMKLGFERKDPYVNDLAEFLKHHKND
                     RSREVHFVNQMLFAAMIEARSCERFRILSEELKDHELRVFYRELMESEARHYTTFLKY
                     ARKYGGKVDVDARWKEFLEFEASLMDKYGKQETIHG"
     gene            1431..2288
                     /locus_tag="PKN22_08165"
     CDS             1431..2288
                     /locus_tag="PKN22_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNS42720.1"
                     /translation="MKLQLKKPLAIFDLEATGLDVTNDRIVEIAILKVEVDGSTKEYV
                     KRVNPTISISAEAQSVHGISNEDVKNEQTFLELAPEIAAFIGDADLAGYNSNKFDIPL
                     LAEEFLRAGSDIDISAKRFVDVQNIFHKMEQRTLVAAYAFYCNKDLSNAHQAMADVTA
                     TWEVLQAQLEKYENLESNVEFLADFTKVGNYDLLDFAGRIAINEKGEAIYNFGKHKGK
                     TVRAVMVEEPGYYGWMMDANFPLYTKQVLKKEMEKIRSEHNNKKQAQKPEDEGDLQAK
                     LEALKNKFK"
     gene            2291..2901
                     /locus_tag="PKN22_08170"
                     /pseudo
     CDS             2291..2901
                     /locus_tag="PKN22_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            complement(2953..>3175)
                     /locus_tag="PKN22_08175"
     CDS             complement(2953..>3175)
                     /locus_tag="PKN22_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42721.1"
                     /translation="ATLHFTEEQKDKVRSLINGIEEKQEYVKSEVNLTAEQKVEFTYK
                     NELSFQQVLKSFLTESQKETYEQMLATEE"
BASE COUNT         1078 a          512 c          621 g          964 t
ORIGIN      
        1 cacctgtaaa cggatttacg gggatttgac cgctaacaaa aataaaattt cctgattgga
       61 tggcttgact atatggagca attggttgtg gagctccttc tatgtggata gcttttttca
      121 tattgtatat ttttctattc tctttattaa ctttgtgcaa gtttaagcat ttaacttgaa
      181 aaattgaagg tactgattat agataattac gattctttta cttacaactt gtatcattac
      241 attcaaaaat gtggggtaga agtggtggta aagcgcaacg atgagttaca actcgccgaa
      301 atggatgcgt acgataaatt agtgctttct cccggacccg gattgccctc tgaaacaacg
      361 ttgatgtttg attgcattga aaaatatgcc aactccaaac caattttagg tgtttgtttg
      421 ggcatgcaag gaattgcaaa ttattatggc ggagaattaa tcaatcaagc aaaagtaaaa
      481 catggtgttg aagcgttggt aaatatcgat acaacctctc cgctttttaa aaatttagat
      541 gagcaaatta ccgttggact ctaccacagt tggtgtgtag atgaacaaac attgccttcc
      601 gattttaaag tgattggcaa atccgccgaa caagtggtaa tggcaataga gcataaaaaa
      661 cttcctattt atggcgttca gtttcacccc gaatccatca tgactcctaa tggtttgcaa
      721 atcattgcta attttttagc gattcaataa aagagtttta tttgcattct acattcattt
      781 tttcaattga tgaataaata gctatctttg tcagaagtct aaaattaata acaagaatat
      841 gctgggatta aaaatgccaa cagacccacg ttggttgaac attgccgaga aaaatttaga
      901 agaagtattg attgatcatg cattttgtga acaaaaagcg gcatcaaatg ccatttctat
      961 gattgttcaa tatccacatt tgacagattt agtggaggaa atgacagcaa tttccatcga
     1021 agaaatgcaa cattttgaaa tggtacacaa agaattgcaa cgacgtggta tgaagttggg
     1081 ttttgaacgc aaagatcctt atgtgaacga tttggcagaa tttttaaaac accataaaaa
     1141 cgaccgctca agagaagttc attttgtcaa tcaaatgttg tttgcagcta tgattgaggc
     1201 aagaagttgt gagcgtttta gaattttgag cgaagaatta aaagatcatg aattacgtgt
     1261 tttttaccgt gaattgatgg aaagtgaagc gcgtcattac actacatttt taaagtatgc
     1321 tcgaaaatat ggaggtaaag tagatgtgga tgctcgttgg aaggaatttt tggaatttga
     1381 agcttcgttg atggataaat acggaaaaca agaaactatt catggataag atgaaattac
     1441 aacttaaaaa accactagca atttttgatt tggaagcaac cggactggat gtaaccaatg
     1501 atcgcattgt agaaattgcc attttaaaag tggaagtgga tggttctaca aaagaatatg
     1561 taaaacgtgt aaacccaacc atttcaattt ccgcagaagc acaaagtgtg cacggcatat
     1621 ctaacgaaga cgtgaaaaac gaacaaactt ttttagaatt ggcaccagaa atagctgcat
     1681 ttattggcga tgctgatttg gctggatata attccaataa atttgatatt ccgctattag
     1741 cggaagaatt tttaagagcc ggaagcgata ttgatatatc tgccaagcga tttgtagatg
     1801 ttcagaacat tttccataaa atggaacaac gcacgttggt ggctgcgtat gctttctact
     1861 gcaataaaga tttatccaac gcacatcaag caatggcgga tgttactgca acatgggaag
     1921 tgctgcaagc acaattggaa aaatacgaaa atcttgaatc aaacgttgaa tttttagccg
     1981 atttcacaaa ggttggtaac tacgatttac ttgattttgc gggccgcatt gccattaacg
     2041 aaaaaggaga agcgatttac aattttggta aacacaaagg caaaacagtt cgtgcagtga
     2101 tggttgaaga acccggctac tatggctgga tgatggatgc taattttcca ctttatacca
     2161 agcaagtttt gaaaaaagag atggaaaaaa ttagaagcga acacaataat aaaaaacaag
     2221 cgcaaaaacc agaggatgaa ggggatttgc aagcaaagtt agaagcatta aaaaacaaat
     2281 tcaaataaaa atgaaaatca tttgtattgg acgaaatttt gctgatcatg caaaagaatt
     2341 gaaaaacgag ttgccaaccg agccgttgtt tttcttaaag cccgacacgg cacttttaag
     2401 agataacgat gctttttatt atccttcgtt ttcaaaagac atccattatg aatgtgaagt
     2461 ggtgattaaa atctgcaaag tagggaagaa cattcaagag aaatttgcac ataaatatta
     2521 cgagcaacta tcgttgggta ttgatttcac agcacgcgat attcaacaaa tttgcaaaga
     2581 aaaagggttg ccttgggaaa aagcaaaggc ctttgaacac agtgcgcctc tttccaatac
     2641 gtttattaat aaaaacgaac tcgatttgac caatttatcc tttcatttag aaaaaacggt
     2701 cagattgttc aacaaggaaa caccgctcaa tggattttta acatcgatgc catcattgct
     2761 tatgtatcgc aattcatgac cttaaaaacg ggcgatttaa tttttacagg aaccccagca
     2821 ggcgtaggtc caatagccat tggtgacagg ttgacaggct atattggcac acaaaaaatg
     2881 tttgattttg aggtgaaata aaaaagacca atcaaaattg actggtcttt agcaattgaa
     2941 tatttttgta gtttactctt cggttgctaa catttgctca taagtttctt tttggctctc
     3001 agtcaagaaa ctttttaata cttgttgaaa agaaagctcg ttcttataag taaattcaac
     3061 tttttgttca gcagttaaat ttacttcact tttaacgtat tcctgttttt cttcaattcc
     3121 attaattaag cttcttactt tatctttttg ctcttcagta aaatgcaatg tagcg
//
LOCUS       DAOQVQ010000270         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_35776_length_3145_cov_3.764078, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000270 DAOQVQ010000000
VERSION     DAOQVQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35776_length_3145_cov_3.764078"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /gene="grpE"
                     /locus_tag="PKN22_08180"
     CDS             <1..110
                     /gene="grpE"
                     /locus_tag="PKN22_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNS42722.1"
                     /translation="IPADENMKGKVIDNVEKGYYLNDKVIRFAKVVVGQ"
     gene            121..1239
                     /gene="dnaJ"
                     /locus_tag="PKN22_08185"
     CDS             121..1239
                     /gene="dnaJ"
                     /locus_tag="PKN22_08185"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNS42723.1"
                     /translation="MSEKRDYYEVLGVSKNADDAEIKKAYRKLAIKYHPDKNPDDKNA
                     EEKFKEAAEAYEVLSDQQKRAQYDRFGHAGMGGAGGFGGGGMNMDDIFSHFGDIFGGH
                     FGGGSFGGARGGGSVRTKGTNLRVKIKLTLEEIANGAHKKIKVSKLVNAEGVTYKTCS
                     TCNGSGRITRVAQTFLGAMQTQSACTTCQGTGKIIDKKPADANAEGLKRKEDIIELDI
                     PAGVEDGMQLSVTGKGNAGPFNGIPGDLIVVIEEIPHAEIRRDGEHLHYDAYVNFVDA
                     VLGESIEIPTISGKVKIKVDPGTQSGKTLRLKGKGLPVLQGYGSGDFFIHINVWTPKK
                     VSKEEKELLEKLRKSENFIPNPDENEKGFFQRVKDMFQ"
     gene            1435..2505
                     /locus_tag="PKN22_08190"
     CDS             1435..2505
                     /locus_tag="PKN22_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNS42724.1"
                     /translation="MQLWNILSKEELEKKWREKGYEWVTISFYQYAKIDNPQVFRDYL
                     YSIWQKIDVLGRVYVATEGINAQIAVPQQNFEQFRKELYEISFLDGVRLNVAVENGDT
                     EFPFLKLTIKVRDKILADGLNDDTFDVTNKGKHLSAEEFNAITNDPNTILIDFRNHYE
                     SEVGFFQNAITPDVDTFRESLPYIEEKYLKGQEDKNLVMYCTGGIRCEKASAWFKHKG
                     FENVYMLNGGIIKYTNDCKEAGLPNKFIGKNFVFDERRAERISDDVVAHCHQCGEPAD
                     THVNCANEACHLLFIQCSSCNEKMEGCCCDDCKEIIKLPADVQRNMRKGLVYSNKIFK
                     KGRSKKLKFKLNNDKPIELKKL"
     gene            2627..>3145
                     /locus_tag="PKN22_08195"
     CDS             2627..>3145
                     /locus_tag="PKN22_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNS42725.1"
                     /translation="MKDTPLHEEFSFLKGPRTRWREFVYTLGVVAQFIKGFRKMHFLG
                     PTITVFGSARFNENESHYKNARELSAMLAKEGFAIMTGGGPGIMEAANRGAKDVGGIS
                     VGCNIVLPHEQKENPYLDKFITIDYFFVRKELLRKYSFAFIIFPGGFGTLDKTFETIT
                     LIQTGKMEKLPLV"
BASE COUNT         1106 a          480 c          641 g          918 t
ORIGIN      
        1 acattcctgc agatgaaaac atgaaaggaa aagtgattga caatgtagaa aaaggatatt
       61 acttgaacga taaagtaatt cgttttgcta aagttgtagt tggtcaataa gacaaacagt
      121 atgagtgaaa aaagagatta ttacgaagta ttaggtgttt ctaaaaatgc ggacgatgca
      181 gaaattaaaa aagcataccg caaattagcc attaaatacc atcccgacaa aaatccggat
      241 gataaaaatg ccgaagaaaa atttaaagaa gctgccgaag cctatgaagt gttgagcgac
      301 caacaaaaac gtgctcaata cgatcgtttt ggtcacgctg gtatgggcgg agctggaggt
      361 ttcggcggag gtggcatgaa tatggacgac attttctctc acttcggtga tatttttggt
      421 ggtcattttg gtggcggtag ttttggtggt gcacgaggtg gtggttctgt tagaacaaaa
      481 ggaaccaatc ttcgtgtaaa aatcaaactt actttggaag aaattgccaa tggtgcgcat
      541 aaaaaaatca aagtcagcaa attagttaat gcggaagggg taacctataa aacatgttca
      601 acttgtaatg gttctggaag aatcacacgt gtggcacaaa catttttagg tgctatgcaa
      661 acccaatcgg cttgtacaac ttgtcaagga actggaaaaa tcatagataa aaaaccagcc
      721 gatgccaatg ccgaaggatt gaaacgcaaa gaagatatta tagaattgga tatccctgct
      781 ggtgttgaag atggaatgca attaagcgtt acaggaaaag gaaatgctgg tccattcaat
      841 ggaattccag gagacttaat tgtagtgatt gaagaaattc cacatgcaga aattcgtaga
      901 gatggcgaac acttgcatta tgatgcttat gtaaactttg tggatgctgt tttgggagaa
      961 tcaattgaaa ttcctaccat ttctggaaaa gtgaaaatta aagtagatcc gggtacacaa
     1021 agtggtaaaa ccttgcgcct aaaaggaaaa ggattgccag ttcttcaggg ttatggttca
     1081 ggtgattttt tcatccatat caatgtttgg acacctaaaa aagtgagcaa ggaagaaaaa
     1141 gaacttttag aaaaactaag aaaaagtgaa aatttcattc ccaatccaga cgaaaacgag
     1201 aaaggatttt ttcaacgtgt gaaagatatg tttcaataaa caaaatgtta agatggagta
     1261 aaaagtaggt ttatcccatc gcattttgtt aattttgaaa aaaaattgta attgtaattt
     1321 attcattatc aataaaaagt gttttttaac aaattttatg actcaaatca tattatttat
     1381 tgatttttgt tatttatctt tgtctcactt tttattttaa atttagaaca tagcatgcaa
     1441 ctgtggaata ttttaagtaa agaggaattg gaaaaaaaat ggcgtgagaa aggttacgaa
     1501 tgggtaacaa tttctttcta tcaatatgcc aaaatagaca atcctcaggt ttttagagat
     1561 tacttatata gtatctggca aaaaattgac gtactcggtc gagtatatgt agcaacagaa
     1621 ggaataaacg cccaaattgc tgttccccaa caaaattttg aacaattcag aaaagaactt
     1681 tatgaaattt cttttttaga tggcgtgcgt ttaaacgtag ctgtagaaaa tggagataca
     1741 gaatttcctt ttctaaaatt gaccatcaaa gttagagaca aaattttggc agacggttta
     1801 aacgatgata cgtttgatgt taccaataaa ggaaagcatc tatcggcaga agagttcaat
     1861 gcaatcacca acgatcccaa caccatttta attgatttta gaaatcacta tgagtcggaa
     1921 gttggttttt tccaaaatgc cattactccg gatgttgata cattcagaga atcattacct
     1981 tatattgaag aaaaatactt aaaaggtcaa gaagataaaa acttggtgat gtattgcacg
     2041 ggtggtattc gttgtgaaaa agcttcggct tggtttaagc acaaaggatt tgaaaatgtg
     2101 tacatgttaa acggaggtat cattaaatac accaacgatt gcaaagaagc aggtttaccc
     2161 aataaattta ttggaaaaaa ctttgtattt gacgaacgaa gagcagaacg catttcggat
     2221 gatgtcgttg cacattgtca tcaatgtggt gaaccagcag acacacacgt caactgtgcc
     2281 aacgaagctt gccatttgtt attcattcag tgtagttcat gcaacgaaaa aatggaaggt
     2341 tgctgttgcg atgattgcaa agagataatt aaacttcctg cagatgtgca acgcaatatg
     2401 cgtaaaggat tggtttatag caataaaata ttcaaaaaag gtcgttctaa aaagttgaag
     2461 ttcaagctca ataacgacaa acctattgaa ttgaaaaaat tatagaaaac catacagcct
     2521 cgatgattcg ggactttttt atttatcgtc atatcgtaat attgcgatat gacgatatca
     2581 atcattttct tacattttta ttatctttag acgctaaaaa ttcagaatga aagacactcc
     2641 tctacacgaa gaatttagtt ttttaaaagg tccacgcaca cgttggcgcg agtttgtata
     2701 tactcttggc gttgttgcgc aattcatcaa aggttttaga aaaatgcact ttttaggccc
     2761 aaccatcacc gtatttgggt cggcacgatt caatgaaaat gaaagtcact ataaaaatgc
     2821 acgtgaactt tctgcaatgt tagccaaaga aggctttgca attatgacag gtggtggtcc
     2881 aggcatcatg gaagcggcca acagaggagc taaagatgta ggtggaattt ccgttggttg
     2941 caacatcgta ttgccgcacg aacaaaaaga aaatccttat ttggataaat tcataaccat
     3001 cgattatttt tttgtgcgca aagaattgtt gcgcaaatat tcgtttgcat ttatcatttt
     3061 tcctggtgga tttggaacat tggataaaac gtttgaaact attaccctaa ttcaaactgg
     3121 taaaatggaa aaactacctt tggta
//
LOCUS       DAOQVQ010000271         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_35897_length_3138_cov_2.622446, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000271 DAOQVQ010000000
VERSION     DAOQVQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35897_length_3138_cov_2.622446"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKN22_08200"
     CDS             complement(<1..835)
                     /locus_tag="PKN22_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42726.1"
                     /translation="MNIRQIKGLIIAVASTFTFTAVHAQKSEIKVDSPEYHQAKANGT
                     LDQYSIVLDLQNQVEIGAAKPDLTNIQKSSRSSDCDCYVTPDATYSLALAPNDDGSSG
                     LINLPFSFNFYGTTYNSLYINNNGNITFGSPLSTYSSTAFPSANNKIIAPFWADVDTR
                     AGNGQVLYKVTPHAIFVNWVNVGYYSMQGDKLNTFQLTLTDGTDPSVPDGKNIAFCYK
                     DMQWTTGSASSGVNGFGGTPATCGANKGDNVGYFLIGRFDHAGIDFDGPLGVNDGISY
                     LD"
     gene            complement(967..2817)
                     /locus_tag="PKN22_08205"
     CDS             complement(967..2817)
                     /locus_tag="PKN22_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS42727.1"
                     /translation="MTVTGPAPNSCVNSDTVVIVAGGYNPIVSIDPAIDLDCATPTTT
                     LVGHVSNSSGNQTGLSFGWTTTNGNMTSNPNSETVDINSPGTYTLTATDIASTCVTSQ
                     VIQINGDLTQPVIDVQTPLGTVVTCTNPIIPIAANSDAGVSGTWTTAAPGNIINVLDS
                     LGYNIANVNTAGTYTYSVTNPTNHCSATQVVTITVNNTLPIANAGPDMEICPSGTITI
                     QGSGSTTSGGTLVYSWDGTDGNVTMYDFSNIPTPSVPTAATYILHVTDQSNGCSASDT
                     MLAYNQIVPVIFADTTVCASSFNIPADSIQTIALGQWSMTPANSGTFSPSTSVLAPTF
                     TPNNGVINVTLTYTNGCGSDNANLIFAPQPSVATPPAFSCNDMSEILTSNSYGPGIWT
                     VQDNPSTAWLEDTALVFVTGPTTNGGSVNENVMVSVIPNAGQYNLTFTDNTCGYSQQI
                     TLNFVDYPWVEINDTTLCLGVQHTLTANAFNATDYSWNTGATTESIQVAQTGNYIVTV
                     SNQCYTAIDTAVITFNACEIEAPNVISISSQAGNNFWHVKADGIADFNCIILNRWGTV
                     VFEYNDATSGWDGKDKQGNLVTEGVYFYKINATREGGEKLVKQGFIHVVH"
     gene            2831..>3138
                     /locus_tag="PKN22_08210"
     CDS             2831..>3138
                     /locus_tag="PKN22_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42728.1"
                     /translation="MVLTTGLVVVCPEIKPVFPPYISVHWRVEPGVAEDPESWKVLVV
                     GSKQLNTAGETTVTFGSAMILTFNTAGGAHTPAEGVNVYGVDPTIDVLIVAGDQVPEI
                     P"
BASE COUNT          984 a          592 c          562 g         1000 t
ORIGIN      
        1 tatctaagta agaaatccca tcattcactc ctaacggtcc atcaaaatca attcctgcat
       61 ggtcaaaacg accaattaag aaatacccaa cattatctcc tttgtttgcc ccacatgttg
      121 caggagtacc accaaatcca ttaactcctg acgaagcaga acctgttgtc cattgcatgt
      181 ctttgtaaca aaaagcaatg ttttttccat ctggcacaga agggtctgtt ccatctgtaa
      241 gcgtcaactg gaatgtattt aatttatctc cctgcatact gtagtaacca acatttaccc
      301 aattcacaaa aattgcatgg ggtgttactt tgtataatac ttgtccattt ccagcacgtg
      361 tatccacgtc agcccaaaac ggggcaataa ttttattgtt agcagatgga aatgctgtag
      421 atgaataagt acttaaaggg cttccaaaag taatattccc attgttattg atatacaatg
      481 agttgtaggt agttccatag aaattaaatg aaaacggcaa gttaattaaa ccacttgaac
      541 catcatcatt tggagctaat gctaaagagt atgttgcatc tggtgtaacg tagcaatcac
      601 aatcagaaga gcgagagctt ttttggatat ttgttaaatc aggctttgcc gcaccaattt
      661 caacttgatt ttgcaaatcc aaaacaattg aatattgatc caatgttcca tttgcttttg
      721 cttggtgata ttctggggaa tccactttta tttcactttt ttgcgcatgc acagctgtaa
      781 aagtaaaagt agaagcaaca gcaataatca atcctttaat ttgtcgaata ttcataatat
      841 ctatttctaa tcattaattt aactacagac tacaaaaata tcaaaatagt tgcgcgaaaa
      901 aaataaaaaa agggataaat gtttattttt ttcatttacc cctttaaaaa atttaaaata
      961 ataacactag tgaactacat gaatgaagcc ttgtttcacc aatttttcac ctccttctct
     1021 agttgcatta attttataga aataaacacc ttccgttacc aaatttcctt gtttatcttt
     1081 tccatcccaa ccgcttgtgg catcattgta ttcaaaaaca acagttcccc aacggtttaa
     1141 gataatacaa ttaaagtctg caatcccatc tgcttttaca tgccagaaat tgtttccagc
     1201 ttgtgatgaa atggaaataa cgtttggagc ttcaatttca cacgcattaa atgtaataac
     1261 agcagtatct atcgctgtat aacattgatt acttactgta acaatgtagt ttccagtttg
     1321 tgcaacttga atgctttcgg ttgtagcccc cgtattccat gaataatctg ttgcgttgaa
     1381 tgcatttgca gttaatgtat gttgaactcc caagcacaac gttgtatcgt tgatctcaac
     1441 ccaaggataa tcaacaaagt taagtgtaat ttgctgagag taaccacacg tattatcagt
     1501 aaatgttaaa ttatattgtc ctgcatttgg aattacagat accataacat tttcattaac
     1561 agagcctcca ttagttgttg gtccagtaac aaatactaaa gctgtatctt ctaaccaagc
     1621 agttgatgga ttgtcttgaa cagtccaaat tcctggccca tacgagttgg atgttaatat
     1681 ttctgacata tcgttacacg aaaatgctgg tggcgtagca acagaaggct gaggtgcgaa
     1741 aattaaattc gcattatctg atccacatcc attggtgtat gtcaatgtta catttatcac
     1801 tccgttatta ggtgtaaatg ttggagccaa tacagaagtt gatggactaa aagttccgct
     1861 atttgcaggt gtcatagacc attgaccaag tgcaattgtt tgaatactat cagctggaat
     1921 attaaatgaa cttgcacaaa cagttgtatc cgcaaaaata acaggtacaa tttgattgta
     1981 tgccaacatt gtatcggatg cagaacagcc attgctttga tctgtaacat gtaaaatata
     2041 ggttgctgcc gttggtaccg atggtgttgg aatgtttgag aaatcataca ttgttacatt
     2101 tccatccgtt ccatcccatg aataaactaa tgttccaccc gatgttgtgc ttccagaacc
     2161 ttgaatagta attgttccag aaggacaaat ttccatatct ggtccagcat tagcaattgg
     2221 caatgtattg tttactgtaa ttgttactac ttgagttgca ctacaatgat ttgttggatt
     2281 tgttacgcta tatgtataag taccagctgt attgacattg gcaatgttgt atcccaaaga
     2341 atcaagcaca ttaatgatat ttccaggtgc tgcggttgtc cacgtaccac tcactccagc
     2401 atcagaattt gctgcaattg gtatgattgg atttgtacaa gttacaacag ttccaagagg
     2461 agtttgcaca tcaattactg gttgtgttaa atctccattt atttgaatta cttgcgaagt
     2521 aacacatgta ctagcaatat ctgtagccgt taatgtataa gttccagggc tattaatatc
     2581 aactgtttca ctatttggat tgcttgtcat atttccattt gtagttgtcc aaccaaaaga
     2641 caatcctgtt tggttaccag aagagtttga cacgtgtcca accaatgttg tagttggtgt
     2701 tgcacaatcc aaatcaattg ctggatcaat agaaacaata ggattataac ctccagcaac
     2761 aatgacaaca gtatcagaat taacacatga atttggtgct ggacctgtta cggtcaaaat
     2821 ataggagcca atggtgttaa ctactggact cgtggttgtt tgtccagaaa taaaaccagt
     2881 gttccctcca tatatatctg tccattggag agttgagcca ggtgtggccg aggatccaga
     2941 aagttggaaa gtgttagttg ttggatctaa acagttaaat actgcaggag aaacaacagt
     3001 tacatttggt tctgcaatga tattaacatt taatacggca ggaggagcac acacacctgc
     3061 agaaggcgta aatgtgtatg gtgttgatcc aacaattgat gtattaattg ttgcaggcga
     3121 ccaagttcct gaaattcc
//
LOCUS       DAOQVQ010000272         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36064_length_3127_cov_3.816732, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000272 DAOQVQ010000000
VERSION     DAOQVQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36064_length_3127_cov_3.816732"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKN22_08215"
     CDS             <1..466
                     /locus_tag="PKN22_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS42729.1"
                     /translation="ISASGVAVVSDSADIANARIEFDNGCVANVTASRISMKNMRKSR
                     FFQKDAYISVDFLSKEMEVVRMETIEGEPNPFDIVFNMGDGKPVKKIYFDKPQLEEVN
                     AIKEELRTFAQAITNNTNTVVTIEDGYEALNVAYQIIEKLNASMTLMSNNLI"
     gene            518..1366
                     /locus_tag="PKN22_08220"
     CDS             518..1366
                     /locus_tag="PKN22_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42730.1"
                     /translation="MKNIFLLFTLSILLGGCYKNIPNPSWIKIDSFSVVANPNISEGE
                     LTLNNFSNGWVYINDKFIGIFELPCKIPVNITGSASIRVYPTILNNGISATKKNYPFT
                     RAHEEVVTLVQNDTITINPVTNYLDGTSFTVEDFQGGATKFTPGTNTTTNLLIESDGN
                     NNYGRVFLNSSANYWSAYMSKAGDNEPFTFPIGSQVFLEIECKNDQPFITTFIYGNTA
                     GTIKEQNNIKISNINKDWKKIYIELSELITASGGSYFWFGFTFTLPEGQTNATVLLDN
                     IKVVYR"
     gene            1363..2766
                     /locus_tag="PKN22_08225"
     CDS             1363..2766
                     /locus_tag="PKN22_08225"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNS42731.1"
                     /translation="MMFSKKKLGALLLIIDWLVAIAAWASFYYVRKVYIEHSEFSIGS
                     AYYPGLFIIPLFWIAIFALQGTYLDARRLHRMKVLSLTFFGSIVGTIVLFFFTLIDDE
                     VDDHTLFYQSILWLFSIHFIYFLIARTIFASILVKSIHARKNGFRTLIIGGNEKAVEI
                     VNELNHMKKSSGQVLVGFVNLNGTDKLLEGTLPYFGKADDKLETIIRKHNIEEVVIAL
                     ENKEHDKIHTLVARMGGSDLKINVLSDMFDILSGSVKMTNIYGALLIEVNADVMPVWQ
                     RVFKRFIDIAASLIAIIILIPFYIVTAILVKLSSPGPVLFFQERIGRNGVPFKIIKFR
                     TMYVDAEKAGPQLSSSTDKRITPIGKIMRKLRLDEFPQFFNVLIGDMSLVGPRPERQF
                     FIDKIVETDPQFLQLTKVRPGITSWGQVKYGYAETVDQMLQRMKYDLLYMKNRTLALD
                     FKIMLYTIGTIIKAKGK"
     gene            complement(2768..>3127)
                     /locus_tag="PKN22_08230"
     CDS             complement(2768..>3127)
                     /locus_tag="PKN22_08230"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS42732.1"
                     /translation="HLVSNFSVILLIGPILEKQLGWQKLLLISVVTTIAIGVVHVVFS
                     DSGLIGASGLVFLYIVLVSLSNSNDKEIPLTFILVVILFLGQEVLASFNIDNISHLAH
                     IGGGVMAILFKYVFRNV"
BASE COUNT         1069 a          562 c          530 g          966 t
ORIGIN      
        1 tatttcggct tcaggagttg cagtcgtaag tgattctgct gatattgcta atgctcgaat
       61 cgaatttgat aacggttgtg tggctaatgt tacagcaagt agaatatcaa tgaaaaacat
      121 gcgtaaatcg cgattctttc aaaaagacgc ctacatttct gttgattttc tttcaaaaga
      181 aatggaggtc gtgcgtatgg aaaccattga aggcgaacca aacccttttg atattgtttt
      241 caatatgggg gatggtaaac ccgtgaaaaa aatctatttt gacaaacctc aactagaaga
      301 agttaacgcc attaaagaag aattacgaac ttttgctcaa gcaattacaa ataatacaaa
      361 cacagtagtt acaatagaag atggctacga agcattaaac gtggcgtacc aaattattga
      421 aaaactaaac gcttcaatga ccctcatgtc aaacaattta atataattaa aaaaccgtta
      481 caataaaagg agttttctag cgttatttgt gtaaactatg aaaaatatat ttttactttt
      541 caccctatcg atattattag gaggttgtta caaaaacatc cccaatcctt catggataaa
      601 aatcgactct ttttctgtag ttgctaaccc caatatttct gaaggtgaac taacgctcaa
      661 taatttctca aacggttggg tctatattaa tgataaattc attggaatat ttgaattgcc
      721 ttgcaaaatt ccagtaaaca tcaccggaag cgcatcaata cgcgtatatc caactatctt
      781 aaacaacggt atttcagcaa caaagaaaaa ttatccattc actagagcac atgaagaagt
      841 agtaactttg gttcaaaacg atacaataac aatcaaccct gttaccaatt atttggatgg
      901 aacaagtttt actgttgaag attttcaagg tggggcaaca aaatttactc caggcacaaa
      961 tacaactacc aaccttttaa ttgaaagcga tggtaacaat aactatggac gtgtttttct
     1021 taattcttcc gctaattatt ggtcggcata tatgagtaaa gctggtgaca atgaaccttt
     1081 tacattccct attggctcac aagttttctt ggaaattgaa tgcaaaaacg accaaccatt
     1141 cattacaact tttatctatg gcaatactgc tggaaccatt aaagaacaaa acaatattaa
     1201 aatttcaaac attaataaag attggaaaaa aatctacatt gaattgagtg aacttattac
     1261 agcttcgggt ggtagctatt tctggtttgg attcacattt accctgccag aaggtcaaac
     1321 aaatgcaact gttttactag acaatataaa agtagtctat cgatgatgtt ttcaaagaaa
     1381 aaattaggcg cgctactttt gattattgac tggcttgtag caattgcagc ttgggcttct
     1441 ttttattatg tccgtaaagt ttacattgaa catagcgagt tttctattgg ctcagcctat
     1501 tatcctggct tgtttattat tcctcttttt tggattgcca tttttgcact acaaggaacc
     1561 tatttagacg ctaggagatt gcacagaatg aaagtgttaa gcctcacatt ttttggttcc
     1621 atcgtcggta caattgtctt atttttcttc actcttattg atgatgaagt agatgatcac
     1681 acactctttt atcaatctat tttatggttg tttagcatcc attttattta tttcttaatt
     1741 gctcgaacca tctttgctag tattttggtt aagagcattc atgcccgtaa aaatggattt
     1801 cgcacgctaa ttattggagg aaatgaaaaa gcggtggaaa ttgtcaacga gctcaatcac
     1861 atgaaaaaaa gtagcggaca agtgcttgtt ggttttgtga acctaaacgg aaccgataaa
     1921 ttattagaag gtactctccc ctattttgga aaagctgatg ataaactcga aaccattatt
     1981 cgcaaacaca atatcgaaga agtggtcatt gcattggaaa acaaagaaca tgataaaata
     2041 cacacattgg ttgcccgaat gggaggtagt gatttaaaaa tcaatgtttt atctgacatg
     2101 ttcgacattc tttctggctc tgttaaaatg accaatatat atggagctct tttaatagaa
     2161 gtgaatgctg atgtgatgcc agtgtggcaa cgtgttttta aacgatttat agatattgcg
     2221 gcatcattaa ttgctataat aatcctcatc cctttttata tagtaacagc tattttggta
     2281 aaactatcat cgccaggacc tgttttgttt tttcaagaaa gaattggcag aaacggtgtg
     2341 ccttttaaaa ttattaagtt cagaacgatg tatgttgatg cagaaaaagc tggtccacaa
     2401 ttatcgtctt ctaccgataa aagaataact ccaattggga aaataatgcg taaattacga
     2461 ttggatgaat ttccacagtt ttttaatgtt ctaatcggag atatgtcgtt ggttggaccg
     2521 cgaccagaac gtcaattctt catcgataaa attgtagaaa cagaccctca atttttgcaa
     2581 ctcaccaaag ttcgcccggg aattacctca tggggacaag taaaatatgg ctatgcagaa
     2641 acggttgacc aaatgttgca acgcatgaaa tatgacttgt tgtacatgaa aaaccgaact
     2701 ttggccttgg attttaaaat catgctttat accattggca ccatcatcaa agccaaagga
     2761 aaataaacta cacgtttcta aaaacgtatt tgaacaaaat agccatcaca ccaccaccaa
     2821 tgtgtgctaa gtgcgaaata ttgtcaatat taaaactcgc caaaacttct tgaccaagaa
     2881 ataatatcac cactaaaata aaggttagag gaatttcttt atcgttggaa ttggataaac
     2941 tcaccaaaac aatgtataga aacaccaaac cacttgcacc aatcaatccg ctatctgaaa
     3001 aaacaacatg cacaacacct attgcaatgg tcgttaccac actaatcaac aataattttt
     3061 gccaacccaa ttgtttttct aatattggcc caataagcaa aatcacacta aaatttgaaa
     3121 ccaaatg
//
LOCUS       DAOQVQ010000273         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36284_length_3113_cov_3.957162, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000273 DAOQVQ010000000
VERSION     DAOQVQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36284_length_3113_cov_3.957162"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..586)
                     /locus_tag="PKN22_08235"
     CDS             complement(116..586)
                     /locus_tag="PKN22_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42733.1"
                     /translation="MKIILFLALSSVFFACGAKKDATNTTKKEQVSINRPDLETMQIK
                     ATVGKQAATSDPIMSIDFVEVKGNTLIIDLTYGGGCKEHQFEVIGSEAIAKSLPPIRG
                     IQLVHHANEDNCRAIVKKRLEVDITDLAYLKEKDSEIYLALDGWDQKILYKYKK"
     gene            complement(658..1557)
                     /gene="xerD"
                     /locus_tag="PKN22_08240"
     CDS             complement(658..1557)
                     /gene="xerD"
                     /locus_tag="PKN22_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNS42734.1"
                     /translation="MSNWEHYINDFIAYLKIEKGLAENSIDAYTRDVNKLRDFSESIP
                     KNATAVQHEDLVQFIAELYDLGLSNRTQARIISGIKQFYNYLFLEEEINDNPAELIEQ
                     PNLGRKLPEVLSIEEIDAMIAAIDLSKAEGERNRAILETLYSCGLRVSELVNLKFEDL
                     HFDKGFVRVIGKGNKERLVPISPSVISEITTYKNNSRAHLPIQPQASKIVFLNRRGAQ
                     LTRVMIFTMIKQTALLAGITKTVSPHTFRHSFATHLLEGGANLRAIQEMLGHESITTT
                     EIYTHLDERFLRDAILTYHPRNK"
     gene            1561..1968
                     /locus_tag="PKN22_08245"
     CDS             1561..1968
                     /locus_tag="PKN22_08245"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNS42735.1"
                     /translation="MKLLIINGPNLNLLGKRDTQIYGSQSFEDYFLELKSKFPTIDFF
                     DFQSNIEGAIIDCLQQTQVDGVIINAGGYSHTSVAIRDCIDAIDVPVVEVHISNVAAR
                     ESFRHESLLTPVCKGCVFGFGMKSYELAVLSFL"
     gene            2115..>3113
                     /locus_tag="PKN22_08250"
     CDS             2115..>3113
                     /locus_tag="PKN22_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNS42736.1"
                     /translation="MKILKLTLLLLGFLFTFNSIYSQCSYTPISKTGWSVAYVDSEET
                     TGEGANNGKAIYAIDGDTTKFWHTQWQGAQPTYPHEIQINLGASHDVSGISILSRYNN
                     PYGKPKQYEIYVSTDGVSWQTAQAIGDFTYPDVNAAAQRTYVYFGAVAAQYVRIKMLS
                     SYGNYYYAVISEIDIYEDLTCPATGQINQLASLDAVPKKYTTDVPFNLNATTNSGLPI
                     TYSVVSGPATIVGNTVTLNGTAGTVVVKAEQAGDGTYYSWEQTQTFQVVDLALIQPQI
                     TTRFTANKDVQMPTLSAYLLHAYTAIDEPAQLSVTALEYIIDGTSYPAVFKNNSWQY"
BASE COUNT          960 a          540 c          554 g         1059 t
ORIGIN      
        1 cattaagtca ttgaattgct ccatcacacc agcgtgtttg tcttcatcaa ttcccagttt
       61 tgataatgtt tctgttacaa aaccattgtt taatagtgag gttaaatcca tcttcttatt
      121 ttttgtattt gtaaagaatt ttttgatccc acccatcaag tgctaaataa atttcactgt
      181 ctttttcttt caaataagcc aaatcagtga tatccacttc taagcgtttt ttaacaatag
      241 cacggcaatt atcttcgttg gcatggtgca ccaattgaat gccacgaatt ggagggagtg
      301 atttagcaat ggcttcactg cctatcactt caaattgatg ttctttgcat ccaccaccat
      361 acgttaagtc aataattaag gtgttgcctt ttacttcaac aaaatctata ctcataatcg
      421 gatcagatgt agctgcttgt ttgcctacag ttgcttttat ttgcatagtt tccaaatcag
      481 gtcgattaat tgatacttgt tctttttttg ttgtgtttgt tgcgtctttc tttgctccac
      541 aagcaaaaaa cacagacgat aatgctaaaa ataggatgat tttcatgatt ttaatttttt
      601 attcctcttt tcaaatataa taccaacaat tgtttttacc aataacttta tttaaaatta
      661 tttatttcta gggtgatagg ttaaaatagc atcccgcaga aaacgttcgt ccaaatgggt
      721 gtagatttct gtcgtggtaa tggattcgtg gcccaacatt tcttgaatag cccttaaatt
      781 ggctccacct tctaataaat gagtggcaaa cgaatgacga aaagtgtggg gcgaaaccgt
      841 ttttgtgatg cctgccaaaa gtgctgtttg tttaatcatt gtaaaaatca tcacacgggt
      901 taattgtgct ccacgacgat ttaaaaaaac aattttactt gcttgcggtt gaatcggaag
      961 gtgggcacgt gagttgtttt tgtaggtggt aatttctgaa ataacgcttg gactaatcgg
     1021 caccaatcgt tctttgtttc ctttcccaat gacacgcaca aatcctttat caaaatgcaa
     1081 atcttcaaat ttaaggttca ccaattcaga aacacgaagt ccacaactgt agagtgtttc
     1141 caaaattgca cggttgcgtt caccttctgc ttttgaaagg tcaatagcag caatcattgc
     1201 atcaatttct tcaatagaca aaacttcggg gagttttcta cctaaattag gttgctcaat
     1261 caattcggca ggattatcgt tgatttcttc ttccaaaaac aaataattgt aaaattgttt
     1321 gatgcctgaa ataatacgtg cttgtgtgcg attgctcaat ccaaggtcgt ataattctgc
     1381 aataaactgc actaaatctt cgtgttgaac ggccgttgca tttttgggga tggattcaga
     1441 aaaatcgcgc aatttgttga catcacgcgt gtaagcatca atagaatttt ctgctaatcc
     1501 tttttcaatt ttcagatatg caataaaatc attaatatag tgctcccaat tcgacatgat
     1561 atgaaacttt taataataaa cggtccaaat ttaaacttac ttggtaaacg cgatacacaa
     1621 atatacggtt cgcaatcgtt tgaagattat tttttagaat taaaaagtaa gtttccaaca
     1681 attgattttt ttgattttca atcgaatatc gaaggagcga ttattgattg tttgcagcaa
     1741 actcaagtag atggggtgat tattaatgct ggaggatact cgcataccag tgttgctatt
     1801 cgtgattgta ttgatgcgat agatgtgcct gttgttgagg tacatatttc caatgttgca
     1861 gcccgcgaat cctttcgcca cgaatctttg ttaacccctg tttgcaaagg atgtgtattt
     1921 ggttttggaa tgaaatctta tgagttggca gttttatcct ttttgtgatt attatttttg
     1981 agtttccaag agttaggtga tttttttatc gcattatttt ttttatttca aataatgtag
     2041 gatatttgta aaacgattaa gtaagctatt taatcctttt tgtaagtaac actaattaat
     2101 atttgttata ggaaatgaaa attttaaaac tcacgttatt attactagga ttcctattca
     2161 cattcaattc aatttattct caatgttcat atacaccgat atctaaaact gggtggagcg
     2221 ttgcttatgt tgatagtgaa gaaactactg gtgaaggtgc taataatggc aaagcaattt
     2281 atgcaattga tggtgataca acaaaatttt ggcacacaca atggcaagga gctcaaccaa
     2341 cttaccctca tgaaattcaa attaatttag gggcaagcca cgatgtgagt ggtatttcta
     2401 tattgagcag atacaataat ccgtatggaa aaccaaaaca atacgaaatc tatgtcagta
     2461 ctgatggggt atcttggcaa actgcacaag caataggtga ttttacatat cctgatgtta
     2521 atgctgctgc tcaacgaacc tatgtttatt ttggagctgt agctgctcaa tatgtacgca
     2581 ttaaaatgtt atcttcttat ggtaattatt attatgctgt aatttcagaa attgatattt
     2641 atgaagattt aacgtgtcca gcaacgggtc aaattaacca actagcaagc ctagatgctg
     2701 ttccaaaaaa gtacacaaca gatgttccgt ttaacttaaa tgcaacaacc aattcgggat
     2761 tgccaattac atattcagta gtttctggtc cagcaaccat tgtaggaaac acggtaacat
     2821 taaacggtac tgctggaacg gtagttgtaa aagcagaaca agcaggtgat ggaacctatt
     2881 attcgtggga gcaaacacaa acatttcaag ttgttgattt agctttaatt cagccacaaa
     2941 ttacaacacg ttttacagca aataaagatg ttcaaatgcc aacattgagc gcttacttgt
     3001 tgcacgcata tacagctatt gatgaaccag cacaattgag cgtaacagca ttagaataca
     3061 taattgatgg aacaagttat cccgcagtat ttaaaaataa ttcttggcaa tat
//
LOCUS       DAOQVQ010000274         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36287_length_3113_cov_3.464356, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000274 DAOQVQ010000000
VERSION     DAOQVQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36287_length_3113_cov_3.464356"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..>3113)
                     /locus_tag="PKN22_08255"
     CDS             complement(288..>3113)
                     /locus_tag="PKN22_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS42737.1"
                     /translation="DAIMSDLPESIKTPSIKKFSFDDLPIVSIAATSDKMSDVEFNDL
                     IDKRIQPLLARIEGVARVISIGGREREFQVNLDAKKLETYNLSILQVQQAILSSNMDF
                     PTGSVKSQEQSMLIRLEGKYKTIEELGNLVIVQSNQSNGQVRLKDIADVQETQQDAEK
                     IARLNRKESIALQVLKQSDANAVSVSEKTQKVLKQIEEEYKGYGVKMQIANDTSLYTL
                     ESADAVVHDLIMAIILVAAIMLLFLHSLRNALIVMIAIPISLIATFIGMFFLGFSLNL
                     MSLLALSLVVGILVDDAIVVIENIYRHMEMGKNRVRASVDGTREIGFTVISITLVIVV
                     VFLPIGLSTGMVANILREFCLTIVIAVLLSLFTSFTIVPWLTSRYGTLEHFSGKNLGG
                     KIILAFEGWLKKFTNWISRILEWALRHKVSTLVIVVTLLISSFGLVIGGFIGAEFFAQ
                     GDRGELLVQVELPKDASLEQTNQMLMKAEEHLSKKPEVKTLITTVGQSSDGMSGISKT
                     LYKGEIAMKLVNKNEREDATNILAAKYKKELSKILVGAKVTTAPISIMGMAESAPLEM
                     AIIGSDINEVYAFADKVLDTLRNIPGVAEAKVSSEAGTPEIAIEIDRDKMAALGLSMQ
                     VVGGTLQTSFNGNTDGKFRRGEYEYDINIRLDAFNRKNIEDVKNVSFVNNMGQIVHLS
                     QFANVELKSGPNKLERRDKTASVKVQSQAVGKPVSAIAAELEAKMAKMDKPLGIDYKW
                     GGDMERQQDGFGSLGIALTVSVLLVYFIMVMLYDSFVHPLVVMFSIPLAIIGAFLALA
                     LTNNLLSIFTILGMIMLIGLVAKNAIILVDFTNHLKSEGKSTHEALILANHARLRPIL
                     MTTIAMVIGMLPIAIASGAGSEWKNGLAWVIIGGLISSLFLTLIVVPVVYQTFDSILN
                     KFGLNKPPLDFEKLTTEPYDHKDVLEY"
BASE COUNT          997 a          645 c          481 g          990 t
ORIGIN      
        1 tgtcaatcac tgatttatca ggaaaagtaa tttctgaacg ttcgtatgaa ttgaatggca
       61 acgttagttt accaatcaat acaacaactt ttgcagaagg aacttacatt gtaacattgc
      121 aaagaaatgg ttctatccaa caaaagaaat tgattgttag ataactcaat tgattttaaa
      181 attgaaaagc cgatgaagtt gattcatcgg cttttttatt ggtaattcac ataaaaaaat
      241 agttgccctt tattgagcaa ctatttttct ttgtagtata tgtaggatta gtattctaac
      301 acatctttat ggtcataagg ttctgttgtt agtttttcaa aatccaatgg tggtttgttt
      361 aaaccaaatt tgtttaaaat actatcgaat gtttgataaa ctacaggaac tacaatcaat
      421 gttaggaaca acgaagaaat caatcctccg ataattaccc aagccaaacc gtttttccat
      481 tcagaacctg cacccgatgc aattgcaatt ggcaacatac caatcaccat ggcaatggta
      541 gtcatcaaga ttggacgcaa acgtgcatgg ttggccaaaa tcaacgcttc gtgggtagat
      601 tttccttcag attttagatg gttggtgaaa tcgactaaga taatcgcatt ttttgccacc
      661 aaaccaatca acataatcat accaagaatg gtaaagatac tcaacaagtt gttggttaat
      721 gccaatgcta aaaacgctcc gataattgcc aaaggaattg agaacattac cacaagtggg
      781 tgcacaaaac tgtcgtacaa cattaccatg ataaaataaa ccaacaatac ggaaacagtt
      841 aaagcgattc ctaatgatcc aaaaccatct tgttgacgtt ccatatcacc accccatttg
      901 taatcaatcc ccaatggttt gtccattttt gccatttttg cttctaattc agcagcaata
      961 gcactaactg gcttaccaac agcttgtgat tgtactttta cagatgctgt tttgtcacgt
     1021 ctttccaatt tattaggtcc agatttcaat tcaacatttg caaattgcga taaatgaacg
     1081 atttgcccca tgttgtttac aaacgataca tttttaacat cttcaatgtt tttacggtta
     1141 aacgcatcta aacgtatgtt gatatcgtat tcatactcac cacgtctaaa tttaccatct
     1201 gtatttccgt tgaatgaagt ttgtaacgtt cccccaacaa cttgcatgct caaacctaaa
     1261 gctgccattt tatcacggtc aatttcaatt gcaatttctg gagttccagc ttcggatgaa
     1321 acttttgctt cggcaacacc tggaatgttt ctcaaggtgt ccaatacttt atctgcaaaa
     1381 gcgtaaacct cattgatatc tgaaccaata atagccattt caagtggagc actttcagcc
     1441 atacccatga tactaatagg cgctgttgtt acttttgctc ccaccaaaat tttagacaac
     1501 tcttttttgt atttagcagc caaaatgttg gtcgcatctt cacgctcatt tttgttaacc
     1561 aatttcatag caatctcacc tttgtagagc gttttgctaa ttcccgacat accgtcggat
     1621 gattgaccaa ctgttgtaat caacgtttta acctctggtt ttttcgataa atgctcttcg
     1681 gctttcatca acatttggtt ggtttgttcc aatgatgcat cttttggcaa ctctacttgc
     1741 accaatagct ctcctctatc tccttgtgca aagaattcag caccgataaa tccaccaatt
     1801 accaatccaa acgatgaaat taacaaggta acaacaatca ctaatgtgga aactttatga
     1861 cgcaatgccc attccaaaat tcgagaaatc cagttggtga attttttcaa ccatccttca
     1921 aatgctaaaa taattttacc acctaagttt ttacccgaaa aatgctccaa agttccataa
     1981 cgagatgtta accatggtac gattgtaaac gatgtaaata aactgagtaa tacggcaatt
     2041 acaatggtta aacagaattc acgtaaaata ttagctacca tccccgtact taaaccaatt
     2101 ggtaagaata ccaccacaat tactaaggta atggagataa cggtaaatcc aatttcacgc
     2161 gttccatcta cagaagctct caccctattt ttacccattt ccatgtggcg ataaatattt
     2221 tcaataacca caatggcgtc atccaccaag attccaacaa ccaatgaaag tgctaataat
     2281 gacattaagt taagtgagaa tcctaagaaa aacattccaa taaaggttgc aatcaatgaa
     2341 attggaatgg caatcataac gattaacgca tttcttaaac tgtgtaagaa caacaacatg
     2401 atagctgcca ccaaaatgat agccataatc aagtcgtgaa caactgcatc tgcagattct
     2461 aatgtgtaca aagatgtatc gtttgcaatt tgcattttaa cgccataccc tttgtattct
     2521 tcttcaattt gctttaaaac tttttgtgtc ttttcactca cagaaacagc attggcatcc
     2581 gattgtttta atacttgaag tgcaatagat tcttttctat ttaaacgagc aatcttttca
     2641 gcatcttgtt gtgtttcttg tacatcggca atgtctttca aacgtacttg tccgttgctt
     2701 tggttggatt gcacaattac caagttaccc aactcttcaa tggttttata tttaccttcc
     2761 aaacgaatca acatgctttg ttcttgcgat ttcacactac ccgtagggaa atccatgtta
     2821 gaagacaaaa tagcttgctg aacttgcaaa attgataagt tatacgtttc caatttttta
     2881 gcatccaaat tcacttggaa ttcacgttca cgtcctccaa tagaaataac acgcgcaacc
     2941 ccttcaattc gtgccaataa aggctgaata cgtttgtcaa tcaaatcgtt aaattctaca
     3001 tcagacattt tatcagaagt tgccgcaatc gaaacaattg gcaagtcgtc gaatgagaat
     3061 tttttaattg acggtgtttt aatggattct ggtaaatctg acataatagc gtc
//
LOCUS       DAOQVQ010000275         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36413_length_3106_cov_3.550639, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000275 DAOQVQ010000000
VERSION     DAOQVQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36413_length_3106_cov_3.550639"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKN22_08260"
     CDS             <1..1031
                     /locus_tag="PKN22_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42738.1"
                     /translation="HLKEHLYPKIDTLGAKEKVKKGSINFIPLFTTVKDAPGLTSDYL
                     MENGLYTWFKYSCRLPGEAPGKDKTASTTWIDTQLRAGTRNGTLNDPGVTLGLDSDTR
                     KFIPGFGVKGLGFQDPKAMTTTIRVPIILGVTQNEDDDENCDPTSGELALFEMNASQF
                     KKLVEAMKVYNKANVTYSSGKGDAVKSERYAYKGYGFVYNLNKDTSRGFDTYQWTKTD
                     IVVSKVQWESKLEEAQKAFAEYKEGAEKYGRYYSHIIDDCVNGQIDFATAEKMVVAEI
                     AAKVLSTIGITFEDSVDGILEAFDVNLPLISLVQGAGISQAGTNPQRVTMGDEEPEPE
                     PKVEKKLF"
     gene            1104..1619
                     /locus_tag="PKN22_08265"
     CDS             1104..1619
                     /locus_tag="PKN22_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42739.1"
                     /translation="MDVAKKLGIAILDKDSLVVYTIEGSPLFQISKILPLVTKDTIIV
                     IEDFSYFSTPNPITTATLNQRLGYIFWRLKETETNVVTYNVNTVRKHLGVSSRKKGEQ
                     KLMVQTQVNQVSQESGIKFTNDETDAIALLLYHIRKQIGFLNKLCIQRLKSEKEKNVS
                     TSKYKETVSKV"
     gene            1576..1884
                     /locus_tag="PKN22_08270"
     CDS             1576..1884
                     /locus_tag="PKN22_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42740.1"
                     /translation="MFLHPNTKKPLVKYRIPLISTLVIEEDEDNLWEERQEYINSNYP
                     DMILSIIQVEETTTFDKIKVILDEYGIATDLGFVLKGVDELVKLGKIEKLSKFTYKLL
                     "
     gene            2004..>2504
                     /locus_tag="PKN22_08275"
     CDS             2004..>2504
                     /locus_tag="PKN22_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNS42741.1"
                     /translation="MKTLDELKYAPFHNHTSYSDGFPTVIKSVNQAVKCNLPGLGITD
                     HGTCAGIPEHWMCCNEHNIKPILGSEIYLRIPESWQELGESRNSKSGRYHATLITTNF
                     EGYKRLIAINNAAHRNMEESRGKKYPITTIDMLQEYAGEGLIALTGCVASVTFHDQIL
                     IAEEYVN"
     assembly_gap    2505..2514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2526..>3106
                     /locus_tag="PKN22_08280"
     CDS             2526..>3106
                     /locus_tag="PKN22_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42742.1"
                     /translation="MPHIITRFDEQQLNGFDRPLYLSEKFGIKTVYSTDAHASTKDEL
                     PLLASYTKAMKGYEFTAASIQSKEDDFKDAVAVIGKEKALIAFNGNDEIFNRIEEVNF
                     KRNFKLPESSKEICVMKEYLKVKFEDDIDEFSGKMAHDGSLITRNILEKQFQQEWNIL
                     EKYEFWGYFAVLWDILKAGHEQGALTVARGSASG"
BASE COUNT         1106 a          441 c          596 g          953 t
ORIGIN      
        1 cacatttaaa ggagcattta taccccaaga tcgacacatt aggtgcaaaa gaaaaagtaa
       61 aaaagggaag tattaatttc attccacttt tcacaacggt taaggatgca ccagggttaa
      121 catcagatta tttgatggaa aacggtcttt atacttggtt taaatattct tgccgtttac
      181 ctggtgaagc tcctggtaag gataagacag cttcaacaac ttggattgat acgcagcttc
      241 gtgctggaac acggaacggt acactgaatg accctggtgt tactcttggt ttagatagtg
      301 atactcgtaa atttattcct ggttttggtg taaaggggct agggttccaa gatccaaaag
      361 ctatgacaac aacaattcga gttccaatta tcttgggtgt tacacagaac gaagatgatg
      421 atgaaaactg tgacccaaca tctggtgaac ttgcattgtt tgaaatgaat gcttcgcaat
      481 ttaagaagct tgttgaagca atgaaagtct ataacaaggc gaatgttact tattcatctg
      541 gtaaaggtga cgcagtaaag tctgaacgat atgcttataa aggctatggc tttgtataca
      601 atttaaataa agatacgtct cgtggttttg acacgtacca atggactaaa acagatattg
      661 ttgtttcaaa agttcagtgg gaaagcaaac tggaagaagc acaaaaggca ttcgcagaat
      721 acaaggaagg tgctgaaaag tatggtcgtt actacagtca tattattgat gactgtgtaa
      781 atggacagat tgactttgcg actgctgaaa aaatggttgt agctgaaatt gcagcaaaag
      841 tgctttcaac aatcggtatt acttttgaag attcagttga tggaatcctt gaagcatttg
      901 atgtaaattt accattaatt tcactagttc aaggtgctgg tattagccaa gctggaacta
      961 atccccaacg tgtgacaatg ggcgatgaag aacctgaacc tgaacctaaa gttgaaaaga
     1021 aattgtttta aatgtaatta ataataggga gtgaataact ccctattatt ccaaaccagg
     1081 aatttgtttt gaatataatc gcattggatg tggcaaaaaa attaggtata gctattcttg
     1141 acaaagattc attagtagtt tatactatcg aaggttcacc tttatttcaa ataagtaaaa
     1201 ttttgccgtt ggttactaaa gatacaataa ttgtaatcga agatttttct tattttagta
     1261 cacctaatcc tataactaca gcaactttga atcaaaggtt aggttatatc ttttggagac
     1321 ttaaagaaac tgaaactaat gttgttacat acaatgttaa tacagtaaga aaacatttag
     1381 gtgtatcatc aagaaaaaaa ggtgaacaga aattaatggt tcaaacccag gtaaatcaag
     1441 tttcacaaga atctggaatt aaatttacta acgatgaaac agatgcgatt gcattattac
     1501 tgtatcatat caggaaacaa attgggtttt taaataaatt gtgcattcaa cgcttaaagt
     1561 cagaaaaaga aaagaatgtt tctacatcca aatacaaaga aaccgttagt aaagtataga
     1621 ataccattaa tttcaactct tgtaatagaa gaagatgaag ataatttgtg ggaagaaaga
     1681 caagagtata ttaattccaa ttatcctgat atgatacttt caattattca agttgaagag
     1741 actacaacgt ttgataaaat aaaagtaatt ttagatgagt atggaattgc tactgattta
     1801 ggttttgttt taaaaggtgt tgacgagctt gttaaattag gcaaaataga aaagctttct
     1861 aaatttactt ataagttatt ataattattc acattattct attcatcgaa aataagctat
     1921 aatttatctt atagcttatt ttttttactc atttgacgga aatcaatgtt tatgatataa
     1981 tgagttattc taaggagtaa aaaatgaaaa cattagatga attaaagtac gctccatttc
     2041 acaatcatac atcatattca gatggatttc caacagtaat aaaatctgta aatcaagcgg
     2101 tgaagtgcaa tcttccaggg ttaggaatta cagatcatgg gacttgcgct ggaattccag
     2161 aacactggat gtgctgcaat gaacacaaca ttaaacccat tcttggctca gaaatttact
     2221 tgcgtattcc tgaaagttgg caagagttag gtgaaagtag aaatagtaag agcggacgtt
     2281 atcatgctac tcttatcact actaactttg aagggtacaa gcggttaatt gcgattaata
     2341 atgcagcaca tcgtaatatg gaagagtcaa gaggcaagaa atacccaatt acaacaattg
     2401 atatgctgca agaatatgct ggtgaaggtt taattgctct tactggctgt gttgcaagcg
     2461 ttacttttca tgaccaaatt cttatcgcag aagaatatgt aaacnnnnnn nnnnatgctg
     2521 agatcatgcc gcacattatt acacgttttg atgaacagca gttaaatggg tttgacagac
     2581 cattatattt atctgaaaaa tttgggatta aaacagttta ttcaactgat gctcatgcat
     2641 caactaaaga tgaactacca ttgcttgcgt catatactaa agcaatgaaa ggctatgagt
     2701 ttacagcagc atctattcaa tcaaaagaag atgattttaa agatgcagtt gcagtaatcg
     2761 gtaaagaaaa agcgttgata gcttttaatg gtaatgatga aatttttaat agaattgaag
     2821 aagtaaattt taaaagaaat tttaaattac cagaatcttc taaagagatt tgtgtcatga
     2881 aggaatatct taaagtaaaa tttgaagatg acattgacga attttctggc aaaatggcgc
     2941 atgatggaag tctaattaca cgcaatattt tagaaaaaca attccagcaa gaatggaata
     3001 ttttagaaaa gtatgaattt tggggatatt ttgctgttct ttgggatata ttaaaagctg
     3061 gtcatgaaca aggtgcatta actgttgctc gtggtagcgc ttcagg
//
LOCUS       DAOQVQ010000276         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36561_length_3098_cov_3.484390, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000276 DAOQVQ010000000
VERSION     DAOQVQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36561_length_3098_cov_3.484390"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKN22_08285"
     CDS             complement(<1..217)
                     /locus_tag="PKN22_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42743.1"
                     /translation="MKFRYFILIVACVSLFKTGVAQSDYLKFRSFKPYTWMIGAGWSF
                     VDNDGRSHSHFIDIKGALHTFRVKRKDG"
     gene            complement(305..1657)
                     /gene="tig"
                     /locus_tag="PKN22_08290"
     CDS             complement(305..1657)
                     /gene="tig"
                     /locus_tag="PKN22_08290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNS42744.1"
                     /translation="MRVIREDIDALNAVLKVEVEAADYDANVKKELERYRKTAKIPGF
                     RPGTVPAAMIKKQYGKGVLAEELNKIVNKALYDYIQENKISILGNPIPKEGVNDKGDF
                     DNPSTFEFSYEIGLSPEISIKLSSKDKFDYNKVKVDDELIGKQISDLQRRYGKLTSAD
                     KTEDTDMIMGLFVELNDDETIKEGGVMHSSTISVEFVEDKATKKALKGLKVGDKVVVD
                     PAKVSRGEKDMAAMLGVTAEQAKELSKKFQLTVNEIKRIELAELNEELFTKLFGPEVV
                     KTEEELKAKIAEDLKNMFANDSDRLLTKSVYEDLMKKTTVELPNEFLKRWIKLSNEKP
                     VTDEQIAAEYNSYAEGLKWQLIQGEIFKSNDIKLDQQEVIDFTKGLLVSNYAQYGIPA
                     PEDAELTKSAMQVLQNKEEVNRIYDMLAESKLTTYFKNTVKLNEKEVSYDDFVALANS
                     "
     gene            complement(1719..1800)
                     /locus_tag="PKN22_08295"
     tRNA            complement(1719..1800)
                     /locus_tag="PKN22_08295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1764..1766),aa:Leu,seq:tag)
     gene            1988..>3098
                     /locus_tag="PKN22_08300"
     CDS             1988..>3098
                     /locus_tag="PKN22_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42745.1"
                     /translation="MKRFIVLFSTIALALILLSGGCDEKKTNSENTTTTMNENKKYTF
                     TDYDASWKKVDSLDAKNLPKSALDLVNQIMAKATSDNNQPMQFKALMYQLKYSQVLED
                     NDFNAILAKINQLQARNQAPLSQLLSSLKAELIWNYYSNNRYQIHNRSQTQNFELNDI
                     TTWDLKKLMNEVFTNFNASLTNKEHNQSIALQAWDYILTNNTPETYLSAPTVYDFLAH
                     RALSVLQNDELSITRPADKFAVNDSTYFGSDKQFLAINTQSEDSLSTQLHYIKTLQEL
                     VRFHQTNNYPVAKVRNQLKRYLFVYQNSTSEQKSTWYERALQSMQKEFANNPSIGEVN
                     AALASLYIDLSANAAKNSPHYSLLKKAHELCTSTIK"
BASE COUNT          965 a          637 c          438 g         1058 t
ORIGIN      
        1 aaccgtcttt tcttttaacc ctgaatgtat gcaatgctcc ttttatatct ataaaatgtg
       61 aatgagagcg accgtcgttg tcaacaaaac tccatcctgc acctatcatc catgtataag
      121 gtttaaacga tctgaatttt aaataatcgg attgtgcaac acctgttttg aacaggctaa
      181 cacatgccac tattagaata aagtatctaa atttcatagg taatagagtt tatttatggc
      241 gaatataaac aaaaaaactc ggaccaaagc ccgagttttc ctagtatctt caaaagaaca
      301 tttcttagct attcgccaat gctacgaaat catcgtatga aacttctttt tcgtttaatt
      361 taacggtatt tttgaagtaa gttgtcaatt ttgactcagc caacatatcg taaatacggt
      421 ttacttcttc tttgttttgc aacacttgca ttgcactttt tgttaactca gcatcttctg
      481 gcgctggaat tccgtattgt gcataattgc tcaccaacaa tccttttgtg aaatcaataa
      541 cttcttgttg gtccaattta atatcatttg atttgaagat ttcaccttga atcaattgcc
      601 attttaaacc ttctgcatac gaattgtatt ctgcagcaat ttgctcatcc gttaccggtt
      661 tttcgttgga taatttaatc catcttttca agaattcatt tggtaattca accgttgttt
      721 ttttcatcaa atcttcgtaa actgattttg tcaacaaacg atctgaatca ttagcaaaca
      781 tatttttcaa gtcttcagca atttttgctt tcaactcttc ttctgtttta acaacttctg
      841 gtccgaataa tttagtaaac aactcctcat tcaattctgc caattcaatg cgtttgattt
      901 cattgacagt caattggaat tttttagaca attcttttgc ttgctcagct gttacaccca
      961 acattgctgc catgtctttt tctcctcttg aaacttttgc agggtcaaca acaactttgt
     1021 cgccaacttt caaacctttc aatgcttttt tagttgcttt gtcttcaaca aactcaaccg
     1081 aaattgtaga tgaatgcatt acaccacctt ctttgattgt ttcgtcgtcg tttaactcaa
     1141 caaaaaggcc cataatcatg tctgtatctt ctgttttgtc agccgaagtt aattttccat
     1201 aacgacgttg caaatcactg atttgtttac caatcaactc atcgtccacc ttaactttgt
     1261 tgtaatcaaa tttgtcttta gacgataatt taattgaaat ttctggtgat aaaccaattt
     1321 catacgaaaa ttcaaatgta gatggattgt caaaatcacc tttgtcattc actccttctt
     1381 ttggaattgg atttcctaaa atactgattt tgttttcttg aatgtaatcg tacaatgctt
     1441 tgttaacgat tttattcaac tcctcagcca aaacaccttt tccatattgc tttttaatca
     1501 ttgcagcagg aacagttcct ggacgaaatc ctgggatttt agctgttttt ctatatcttt
     1561 ctaattcttt tttaacgttt gcatcataat ctgcagcttc tacttctact ttcaatactg
     1621 cattcaatgc atcgatgtct tcacgaataa ctctcatcta aatcaaattt taatcaataa
     1681 aaaatggcat aagtagcact tacaccattt agttttcgtg cggatggagg gactcgaacc
     1741 cgcacacctc tcggcactag atcctaagtc tagcgtgtct accaatttca ccacatccgc
     1801 aattgtttat ttcaatggta aagtgctcat caccttacac ccaactttct tttgggtttg
     1861 caaagatact atttttttat tttaaacaag gggttgacca ctttttttta atttttagaa
     1921 aaataatttg aaccaaaacg cattttaagt gtatggatta ctatactttt ataaaaaaca
     1981 aatggctatg aaacgtttta ttgtcctatt ctctacaatt gcacttgctc ttatcttgct
     2041 ttcgggtggc tgcgatgaga aaaaaacaaa ttcagaaaat acaaccacaa ctatgaatga
     2101 aaataaaaaa tatactttta cagattacga tgctagctgg aaaaaagtgg actcgctaga
     2161 tgctaaaaat cttccaaaat ctgcccttga tttggttaat caaattatgg caaaagcaac
     2221 atctgacaat aatcaaccga tgcagttcaa agcactgatg tatcaattaa aatacagtca
     2281 agtgttggaa gacaacgatt tcaatgccat tctagctaaa atcaatcaat tgcaagcacg
     2341 caaccaagca cccttatcgc aacttttatc ttctttaaaa gctgaattga tttggaatta
     2401 ttattccaac aaccgttatc aaattcacaa ccgttcacaa acgcaaaatt ttgagttgaa
     2461 cgatattaca acatgggatt taaaaaaatt aatgaacgaa gtttttacaa atttcaatgc
     2521 ttcattaacc aataaagaac acaatcaatc tattgcgttg caagcttggg attacattct
     2581 caccaacaat acgccagaaa cctatctctc tgcaccaaca gtttatgatt tcttggctca
     2641 ccgagcattg tctgttttgc aaaatgacga actttcaatt acccgcccag ccgataaatt
     2701 tgcggttaac gatagcacct attttggctc cgataaacag ttcttggcca tcaatacaca
     2761 atcggaagat tcgctttcaa cacagctaca ctacataaaa accttgcaag aattggtgcg
     2821 ctttcatcaa acaaacaatt atccagttgc caaagtgcgc aatcaattaa aacgttattt
     2881 atttgtttat caaaacagta cttcggagca aaaatcaact tggtatgagc gcgcattgca
     2941 atctatgcaa aaagaatttg caaacaatcc ttctatagga gaagtcaacg ccgctttggc
     3001 cagtttgtat attgatttaa gcgccaatgc agctaaaaac agccctcatt attcactatt
     3061 gaaaaaagca catgagctgt gtacttctac tattaaaa
//
LOCUS       DAOQVQ010000277         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36600_length_3096_cov_2.556396, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000277 DAOQVQ010000000
VERSION     DAOQVQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36600_length_3096_cov_2.556396"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKN22_08305"
     CDS             <1..261
                     /locus_tag="PKN22_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNS42746.1"
                     /translation="FRVDKQFFKNKPMLLLSTTTVENGSVNVMKTATTFFPLLGADLR
                     ESFIFPNFNENFNSEKNVIVNESLKNELNEIVQRFKQGININ"
     gene            643..>3096
                     /gene="gcvP"
                     /locus_tag="PKN22_08310"
     CDS             643..>3096
                     /gene="gcvP"
                     /locus_tag="PKN22_08310"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNS42747.1"
                     /translation="MNKFSRRHIGPNEVEQASMLKTIGVASVAELIDKTIPAHIRLNG
                     ELAIADALTEQEYLQHIAQKADKNKIFKSFIGMGYNETITPSVILRNVLENPGWYTAY
                     TPYQAEISQGRLEALLNFQTMILDLTGLELANASLLDEASAASEAMIMFYNSRSREEI
                     KNGVNKFFIAEDVFPQTKDVVLGRAKNLGIELVEGNIDTFNSDGTYFGALVQYPNKNG
                     EIKDVKGFTARQKENGVKVAVVADILSLVLLEAPGKFGADVVCGSSQRFGVPMGFGGP
                     HAAFFATKEDYKRHIPGRIIGVSRDADNNIALRMALQTREQHIKRDKATSNICTAQAL
                     LAVMAGMYAVYHGPNGLKVIAQNIHTLACTAANGLKESGFNVSETPFFDTIWIKGVNV
                     SKIKALAEEKEMNFNFPTSSDVTLSFGETHTIEDVKTILEIFAAAEGKSVGSTSNNTS
                     SIQQFLRKDEILTHPTFNSYHSESLMMRYLKRLENKDLSLVHSMISLGSCTMKLNAAS
                     EMMPITNPKFANLHPFAPADQVQGMVEVIKQLEKDLCASTGFAGVSVQPNSGAQGEYA
                     GLMVIKAYHESRGDFNRTKIIIPSSAHGTNPASAVMAGFDVVVTGCDEKGNINIDELR
                     AKAVELKDVLAGIMVTYPSTHGVYEEGIKEITSIIHENGGQVYMDGANMNAQVGLTNP
                     GNIGADVCHLNLYKTFAIPHGGGGPGVGPIGVAAHLVDFLPTNAVVKTGGDKAIHGIS
                     SAPYGSALVLLISYGYIKMLGAKGLRNATETAILNANYIAASLKNYYPILYTGTNGTV
                     AHELILDCRDLKRTSEVEVA"
BASE COUNT         1040 a          483 c          592 g          981 t
ORIGIN      
        1 tttagagtag ataaacaatt ctttaaaaac aagccgatgt tattgctttc cactacaaca
       61 gtggaaaacg ggagtgtaaa tgtgatgaaa acagcaacta catttttccc attactcggt
      121 gcagatttaa gagaatcatt tatcttccct aatttcaatg aaaatttcaa ttcagaaaaa
      181 aatgtgattg taaacgagtc attgaaaaat gaattgaatg aaatcgttca gcgctttaaa
      241 caaggaatta atatcaatta atttatattc agagttaaat gaaaaagagg ctgttctaaa
      301 ttttgagaca gcctcttttt tttctttgct agaagaaaat aattaaataa ccagtttaac
      361 tacagtaata acgaaatggt taatgtgttg ttacattatt tttttgataa ttgtatgaag
      421 tgtagctttg gttgattaaa tagcaattct gattgaatat tttgttttta taacaaaagc
      481 agttgatatg tcaatatcga actatttcga tatcgacata tttcgatatc agcatataat
      541 taaactgttt tttttcacac tttaagccca aatatttact atttaatact tattaatttt
      601 gtatctttgc aaccaaaatt tttaatttaa taatacgttc aaatgaataa attttctcgc
      661 cgtcacattg gacctaatga agtagagcaa gcttcaatgt tgaaaacaat tggtgtagct
      721 tcagttgccg agcttattga taaaacaatt ccagctcata ttcgcttaaa tggtgagttg
      781 gcaattgcag atgctttgac tgaacaagag tatttacaac acatcgctca aaaagcagat
      841 aaaaataaaa ttttcaaatc atttattgga atggggtata atgaaacaat tactccttct
      901 gttattttgc gcaatgtttt ggaaaatcca ggatggtaca cagcttatac tccttaccag
      961 gcagaaattt ctcaaggacg tttagaagcg ttgttgaatt tccaaacaat gattttggac
     1021 ctaacaggct tagagttagc taacgcatct ttgttagatg aagcatccgc tgcttcagaa
     1081 gcaatgataa tgttttataa ctcaagatca agagaggaaa ttaaaaacgg tgttaataaa
     1141 ttctttattg cagaagatgt tttcccgcaa acaaaagacg ttgttttagg tcgtgcaaaa
     1201 aacttaggaa ttgaattagt agaaggtaac atcgacacgt ttaattctga tggaacatat
     1261 tttggtgcct tggtgcaata tcctaacaaa aatggtgaaa ttaaagatgt gaaaggattt
     1321 acagctcgcc aaaaagaaaa tggtgtaaaa gtagctgtag ttgccgatat tctttcttta
     1381 gttttgttag aagctccagg taaatttggt gcagatgtag tttgtggttc ttctcaacgc
     1441 tttggtgtgc caatgggctt tggtggacct catgctgcgt tctttgcaac aaaagaagat
     1501 tacaaacgcc atattccagg tagaattatt ggtgtttctc gagatgctga taacaatatt
     1561 gccctaagaa tggcattgca aacacgtgag caacatatca aacgtgataa agcaacttca
     1621 aatatctgta cggctcaagc attgttagct gttatggctg gaatgtatgc tgtatatcac
     1681 ggaccaaatg ggttgaaagt aattgctcaa aatattcaca cgttggcttg cactgctgca
     1741 aatggattga aagaaagcgg atttaacgta agtgaaacac cattttttga tacaatttgg
     1801 attaaaggtg taaacgtttc aaaaatcaaa gcgttggcag aagaaaaaga aatgaacttc
     1861 aacttcccaa ctagctcaga tgtaacatta agcttcggtg aaacacatac aattgaagac
     1921 gttaaaacta ttttagaaat atttgcagca gcagaaggaa aatcagttgg aagcacgtca
     1981 aataacactt catctattca acaattttta agaaaagatg aaattttaac acatccaact
     2041 tttaattctt atcattcaga atcgttaatg atgcgctact tgaaacgtct tgagaataaa
     2101 gacttgtcat tagtgcactc aatgatttca ttaggttctt gtaccatgaa attgaatgcc
     2161 gcttcagaaa tgatgccaat caccaaccct aaatttgcca acttacaccc atttgcgcca
     2221 gcagatcaag tgcaaggaat ggttgaagtt atcaaacaat tggaaaaaga tttgtgtgct
     2281 tcaactggat ttgcaggtgt ttctgttcag ccaaactctg gtgcgcaagg tgaatacgct
     2341 gggttaatgg tgattaaagc ttaccacgaa tcaagaggtg attttaatag aacaaaaatt
     2401 atcattcctt catctgctca cggaacaaac cctgcatctg ctgtaatggc tggatttgat
     2461 gtggttgtta ctggttgcga tgaaaaagga aatatcaata tcgatgaatt acgtgctaaa
     2521 gcagttgaat taaaagatgt tttagcgggt attatggtaa catatccttc aactcatgga
     2581 gtttacgaag aaggtatcaa agaaattaca tcgattattc acgaaaatgg cggacaagtt
     2641 tacatggatg gtgcaaacat gaacgcacaa gttggtttga ctaatccagg taatattgga
     2701 gctgatgttt gtcacttgaa cttgtataaa acatttgcaa ttcctcatgg tggaggtgga
     2761 ccaggagttg gaccaattgg tgttgccgct catttagttg attttttacc aactaatgca
     2821 gttgttaaaa cgggtggaga taaagctatt cacggtattt cttctgcacc ttatggaagc
     2881 gctttggtgt tgttgatttc ttatggatac atcaaaatgt taggtgctaa aggattgaga
     2941 aatgctactg aaacagcaat cttaaatgct aattacattg cagcatcttt gaaaaattat
     3001 tatcctattt tgtacactgg aactaatgga acagtagcac acgaattaat tttggattgc
     3061 cgtgatttaa aacgtacttc agaagtggaa gttgcc
//
LOCUS       DAOQVQ010000278         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_36916_length_3079_cov_2.900132, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000278 DAOQVQ010000000
VERSION     DAOQVQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36916_length_3079_cov_2.900132"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKN22_08315"
     CDS             complement(<1..573)
                     /locus_tag="PKN22_08315"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNS42748.1"
                     /translation="MQMRSIIEAAWENRELLNNSETIQAINQVIEDIDKGVLRVAEPL
                     ENGEWQVNEWVKKAVVMYFPIRKMETIEVGPFEFHDKMALKRNYAELGVRVVPHAIAR
                     YGAYVAPGVIMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     LQAAPVIIEDNAFIGSRCIVVEGVRVGKEAV"
     gene            complement(622..999)
                     /locus_tag="PKN22_08320"
     CDS             complement(622..999)
                     /locus_tag="PKN22_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNS42749.1"
                     /translation="MKQFFLLILLFVALHIQAQPVMKVDKALHVFPDAKEGELLTHYY
                     KITNVGNQPLLLSEYKVACSCTKVELPNKPILPQETYSLKLTFDTHAKYYQQDRTVLL
                     QTNTKKNTEKLRFKAFVIPKEEK"
     gene            complement(1063..1506)
                     /locus_tag="PKN22_08325"
     CDS             complement(1063..1506)
                     /locus_tag="PKN22_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNS42750.1"
                     /translation="MKKLLFSIALTAVAAFSAKTAVAQEVTAGPKIEFKKDTHDYGNI
                     KYGADGSCSFEFKNTGNAPLIISNAKGSCGCTVPEWPKEPINPGKTGVIKVKYDTKRP
                     GVINKSVTITSNAVDEPTKILRIKGEVGPRPESATPVNTSGAPAN"
     gene            complement(1620..2927)
                     /gene="murA"
                     /locus_tag="PKN22_08330"
     CDS             complement(1620..2927)
                     /gene="murA"
                     /locus_tag="PKN22_08330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNS42751.1"
                     /translation="MASFEIYGGKPLKGELIPQGAKNEALQVLCAPLLTAEKVTISNI
                     PDIVDVNKLINLLQAMGVKVEKVETGTFIFQAKNIDLDYLKSEDFKNRASSLRGSIMI
                     VGPLLARFGQGFIPKPGGDKIGRRRLDTHFEGFAMLGAKFNYDAGEHFYSVEAKKLKG
                     TYIHLDEASVTGTANVVMAAVMAEGKTTIYNAACEPYLQQLCKMLNLMGAKISGIGSN
                     MLHIERVKELGGCYHRILPDMIEIGSFIGLAAMTKSEITIKDVSWDNLGIIPNVFRKL
                     GIQLERRGDDIFIPAQDIYEIDTFIDGSILTIADAPWPGFTPDLLSIILVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLERKHALKGISMTSPDIRAGV
                     SLLIAALSAEGKSVIHNIEQIDRGYQNIEYRLNNIGADIRRIG"
BASE COUNT          915 a          642 c          481 g         1041 t
ORIGIN      
        1 cactgcttct ttaccaacac gaacgccttc aacaactatg caacgtgaac caataaaagc
       61 attatcttca atgattacag gagctgcttg caaaggttcc aatacacctc caattccaac
      121 tccacccgat aagtgaacgt ttttaccaat ttgtgcacat gaaccaactg ttgcccacgt
      181 gtcaaccatt gttccagagt ctacataagc accaatgtta acatacgaag gcatcataat
      241 aacgcctgga gcaacgtacg caccataacg cgcaattgca tgtggcacaa cgcgcacacc
      301 caattcggca taattgcgtt tcaaagccat tttatcatga aattcaaacg gaccaacttc
      361 aatggtttcc atcttacgaa taggaaaata catcacaacc gcttttttca cccactcgtt
      421 tacttgccac tcgccatttt ccaatggttc ggcaaccctt aaaacgcctt tgtcgatgtc
      481 ttcaattact tgattgattg cttgtattgt ttctgaattg tttaacaact cacgattttc
      541 ccatgccgct tctatgatag atctcatttg aatttgaatt aaattttgag caaaaataag
      601 aaaaccaacc caaaatgcat gttatttttc ttctttcgga ataacaaatg ctttaaatct
      661 taacttttcg gtgtttttct tggtatttgt ttgaagcaac acggttctat cttgctgata
      721 atacttcgca tgcgtgtcaa acgttaactt taacgaatag gtttcttggg gtaaaatagg
      781 tttgttgggc agttctactt ttgtgcacga acatgcaact ttatattccg acaacaacaa
      841 aggttgattg cctacattgg tgattttata ataatgtgtt agtaattctc cttctttagc
      901 atctggaaaa acatgtaatg ctttgtccac cttcattaca ggctgagctt gtatatgcaa
      961 ggcaacaaac aaaagtatca gcaaaaaaaa ttgtttcata cagtaaatat aataaaaaaa
     1021 gctacccaat tggatagctt ttctatgaat tttatctatt gattagtttg ctggtgctcc
     1081 tgaagtatta actggagttg cgctttctgg acgcggacca acttcacctt taattcttaa
     1141 gatttttgta ggttcatcaa cagcatttga agtgatagtt acacttttgt tgattacacc
     1201 tgggcgttta gtgtcgtatt taactttgat aacacctgtt tttcctgggt taattggttc
     1261 ttttggccat tcaggaactg tacatccaca agatccttta gcgtttgaaa taatcaaagg
     1321 tgcattacct gtgtttttga attcaaatga gcaagaacca tctgctccgt atttaatatt
     1381 accgtaatcg tgtgtgtctt ttttgaactc gatttttgga ccagctgtta cttcttgagc
     1441 aactgctgtt tttgcagaaa atgcagctac tgctgtcaat gcaattgaga ataataactt
     1501 tttcatgttt tttcgttttt aatttagttt tacactacca aagatagtac ctattttgtt
     1561 ttagcttctt tttttaacag cgatttaaca gctaatttaa ttttctgtta acgcttcaat
     1621 tatccaattc tacgaatatc cgcaccgata ttattcaatc ggtattcaat attttgatac
     1681 cctctatcta tttgctcaat gttgtgaatg acacttttac cttcggcaga aagagcggcg
     1741 attaacaaag aaactcctgc acgaatatca ggtgaagtca tcgaaatacc ttttaaagca
     1801 tgcttgcgtt ccaacccaat cactgttgct ctgtgtgggt cacaaagaat aatttgagca
     1861 cccatgtcaa ttaatttgtc aacgaagaac aaacgtgatt caaacatttt ttggtgaatc
     1921 aacacgcttc ctttggcttg ggttgccact actaaaataa tggaaagcaa atcgggggta
     1981 aatccaggcc atggagcatc ggcaattgtt aaaattgaac catcaataaa cgtatcaatt
     2041 tcataaatat cctgagccgg aataaaaata tcgtcgcctc tacgctccaa ttgaattcct
     2101 aattttctaa aaacattagg aatgattcct aaattgtccc aacttacatc tttaatagta
     2161 atttcagact ttgtcatggc agccaatcca atgaaactac caatttcaat catatctggt
     2221 aaaatacggt gatagcaacc acccaattct ttcactctct caatgtgcaa catattggaa
     2281 ccaattcctg aaatttttgc acccatcaag tttaacattt tacacaactg ttgcaaataa
     2341 ggctcacaag cagcattgta aatagtcgtt tttccttcag ccatcacagc cgccatcaca
     2401 acgtttgctg taccagttac cgaagcttcg tccaagtgaa tgtatgttcc ttttaatttt
     2461 ttagcttcta ccgaataaaa atgctctcct gcatcgtagt taaactttgc cccaagcatt
     2521 gcaaaccctt caaagtgggt atctaaacga cgacgaccaa ttttatcgcc tcctggtttg
     2581 gggataaatc cttgaccaaa acgtgccaac aatggaccaa caatcataat tgatccacgc
     2641 aaagaacttg ctctgttttt aaaatcttcc gactttaaat agtctaaatc aatatttttt
     2701 gcttgaaaaa taaacgttcc agtttctact ttttccactt tcacacccat tgcttgcaac
     2761 aaattgatta atttattgac atccacaata tctggaatat tagaaatggt aactttttca
     2821 gctgttagta aaggtgcaca taaaacttgc aatgcttcgt tttttgctcc ttgtggaatt
     2881 aattctcctt taagtggttt tcctccgtag atttcaaaag aagccatagg ctattatttt
     2941 ttaaatttct tctgtttcgg ctaatattgt ttgtatgcct tttcatgcgc ttctttaatt
     3001 tttttaacca atgattgtgg ttcaatcact ttcatactgt cgccaaatga aagtaattcc
     3061 atgattaaat cataagtta
//
LOCUS       DAOQVQ010000279         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37209_length_3062_cov_3.560359, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000279 DAOQVQ010000000
VERSION     DAOQVQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37209_length_3062_cov_3.560359"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKN22_08335"
     CDS             <1..425
                     /locus_tag="PKN22_08335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNS42752.1"
                     /translation="QATTTTAAEHMGNYWKTKLELTDLFLTDGSGLSRSNAVSALAFC
                     RLLTYMQQATDAPIYFESLPISGESGTLKNVAKNQAASGKIHAKSGTMKRVKSYAGYA
                     ETQSGRRIAFAFILNNYTCTSKQATQQLEILMNELVGM"
     gene            complement(461..913)
                     /locus_tag="PKN22_08340"
     CDS             complement(461..913)
                     /locus_tag="PKN22_08340"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNS42753.1"
                     /translation="MNLAIIIMTTVLVITLFSPIGVYLGINAAKKKNLKSHRKIQNVI
                     FIICVLGVLVLEGLIKSVGGSGSLASQSDYYETNFFKYTLFSHIIVAILSYLIWAILI
                     VVSNIAYTKSLPGKLSNFHKIVGFVIFIGLVYTAITALLVYLMTLNLI"
     gene            complement(1022..1972)
                     /gene="ftsY"
                     /locus_tag="PKN22_08345"
     CDS             complement(1022..1972)
                     /gene="ftsY"
                     /locus_tag="PKN22_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNS42754.1"
                     /translation="MAFFGLFSKEKKESLDKGLEKTKQSFLSKLARTIVGKSKVDDEV
                     LDNLEEVLITSDVGVETTLKIIERIEERVARDKVLGADELNSILKEEIAALLEENKST
                     DGTNFEVEKKDGLPHVVMVVGVNGVGKTTTIGKLAHQYKQAGKKVVLGAADTFRAAAV
                     DQLIIWSERVGVPIVQQGMGADPASVAFDTLQSAKSQNADIVFIDTAGRLHNKVNLMN
                     ELSKIKRVMDKVIPGAPHEVLLVLDGSTGQNAYEQAKQFSLATDITALAITKLDGTAK
                     GGVVIGISDQMKIPVKYIGVGEKMEDLQVFDKKEFVDSLF"
     gene            complement(2046..2198)
                     /locus_tag="PKN22_08350"
     CDS             complement(2046..2198)
                     /locus_tag="PKN22_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HNS42755.1"
                     /translation="MAKKVVASLQKGGGKAHTKVIKMSKNAKGTYSFKEEVVLNEKVK
                     EWFDKN"
     gene            complement(2208..2393)
                     /gene="rpmG"
                     /locus_tag="PKN22_08355"
     CDS             complement(2208..2393)
                     /gene="rpmG"
                     /locus_tag="PKN22_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNS42756.1"
                     /translation="MAKSKENRIQVILECTEHKESGMPGTSRYITTKNRKNTPDRIEI
                     KKYNPILKRYTVHKEIK"
     gene            complement(2448..2684)
                     /gene="rpmB"
                     /locus_tag="PKN22_08360"
     CDS             complement(2448..2684)
                     /gene="rpmB"
                     /locus_tag="PKN22_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNS42757.1"
                     /translation="MSKVCQLTGKSVMVGNNVSHSNRKTKRRFLPNLVVKKFYLPEED
                     RFITLKISTSALRTVNKKGISACVKEAREKGYLK"
     gene            complement(2823..>3062)
                     /locus_tag="PKN22_08365"
     CDS             complement(2823..>3062)
                     /locus_tag="PKN22_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42758.1"
                     /translation="DKFCQWLLEDFEYNGQTVMMAPATGFYSTKGIGTNQVRLAYVLN
                     KESLTNAIACLKVALEQYPGRTEVQAATKTLSSIG"
BASE COUNT          920 a          583 c          483 g         1076 t
ORIGIN      
        1 tgcaagcaac cacaactact gctgcagaac acatggggaa ttattggaaa acaaaattag
       61 aattaacaga tttgttttta accgatggaa gtggcttatc gcgctccaat gctgtttctg
      121 ctcttgcttt ttgtcgtttg ttgacctata tgcaacaagc tacagatgca cccatttatt
      181 ttgaatcatt gccaatttcg ggtgaaagtg gtacgttaaa aaatgtagca aaaaatcaag
      241 ctgcgagtgg aaaaattcat gcaaaaagtg gcactatgaa acgtgtaaaa tcgtatgcag
      301 gatatgccga aacacaatcg gggcgaagaa ttgcttttgc atttattttg aataactata
      361 cctgcaccag caagcaagct acacaacaat tagaaatttt aatgaatgaa ttggtgggaa
      421 tgtaactaac tcttgttctc tggtatttta aaataactac ttaaattaaa ttcaatgtca
      481 ttaaatatac caagagtgca gttattgctg tgtagacaag tccaataaaa attacaaagc
      541 caacaatttt atggaaattt gacaatttac caggaagact tttggtataa gcaatatttg
      601 aaactacaat taaaatggcc caaattaaat aggataaaat ggcaacaatt atatgtgaaa
      661 ataaggtgta tttaaagaaa ttagtttcgt aataatcgct ttgggaagcc aagctaccag
      721 agcctccgac agatttaatt aacccctcta acactaatac tcccaaaaca caaataataa
      781 aaattacgtt ttgtattttg cgatgagatt ttagattctt ttttttagct gcatttatac
      841 ctaagtatac accaattgga gagaataatg taatgactaa caccgtagtc ataatgataa
      901 ttgctagatt catattgtag tttattaaaa attggtatca aaattacgta tagttttttg
      961 atttctgcat tcaatgagaa ataattactg gtaataaaaa aggggggtac aagcgtaatg
     1021 tttaaaacaa cgagtcaaca aattcttttt tgtcaaacac ttgtaaatct tccatttttt
     1081 cacccacacc gatgtatttt actgggattt tcatttggtc agaaatgcca atcaccacac
     1141 cacctttggc tgtgccgtct aatttggtaa tggcaagtgc tgtaatgtct gttgccaaag
     1201 aaaattgttt ggcttgttca taagcgtttt gacctgttga gccgtccaaa accaacaaaa
     1261 cttcgtgtgg agcgcctgga atgactttgt ccatcacccg tttgattttt gataactcgt
     1321 tcatcaagtt aactttgttg tgcaagcgac ccgccgtatc aatgaaaacg atatctgcat
     1381 tttgagattt tgccgattgc aacgtgtcaa atgccaccga tgcggggtcg gcacccattc
     1441 cttgttgaac aattggaaca ccaacgcgct ccgaccatat aattaattga tcaactgctg
     1501 ctgctctaaa cgtatcggct gcacctaaaa cgactttttt tccggcttgt ttatattgat
     1561 gtgctaattt tccgatggta gttgtttttc caacaccatt aactcccacc accattacaa
     1621 cgtgtggaag tccgtctttt ttctcaacct caaaattagt tccatccgtt gatttatttt
     1681 cttccaaaag ggcagcaatt tcctctttta agatagaatt taattcatct gctcccaaaa
     1741 ctttatctct ggcaacacgt tcttcaatgc gctcaataat ttttaacgtt gtttctacac
     1801 ccacgtcaga agtaatcaat acttcttcaa ggttgtccaa tacttcgtcg tcaacttttg
     1861 attttccgac aatggtgcgt gccagcttgg ataaaaaact ttgttttgtt ttctccaaac
     1921 ctttgtccaa agattctttt ttttcttttg aaaataaacc gaaaaaagcc atagtttaaa
     1981 tataacaaaa gctttcactc aacaatagaa tgaaagctaa tcttttagaa tttatcaatt
     2041 aagaattagt ttttgtcaaa ccactcctta actttctcgt taagaactac ttcttctttg
     2101 aatgaataag ttccttttgc gtttttagac attttaatca cttttgtgtg agccttacct
     2161 ccaccttttt gaagtgatgc tactactttc tttgccatga ttcaatctta tttaatctcc
     2221 ttatgaaccg tgtaacgttt caaaatagga ttgtattttt taatttcaat tctgtccggt
     2281 gtgttttttc tattcttcgt tgtgatataa cgagaagttc ccggcattcc tgattctttg
     2341 tgctctgtgc actcaagaat aacttgaatt ctattttctt ttgactttgc cattttctaa
     2401 ttgtttacgc cttacccttt aagcatcttc tgggaagccg gattatatta ttttaaatat
     2461 cctttttcac gtgcttcttt tacacacgcg ctaattcctt ttttatttac cgttctcaat
     2521 gctgaagtag aaatcttcaa cgtgatgaaa cgatcttctt ctggaagata aaattttttt
     2581 actaccaagt ttggtaaaaa tcttcttttt gttttgcggt ttgagtgaga aacattattt
     2641 cctaccatta ccgactttcc tgtaagttga caaacttttg acatttctta attgtattaa
     2701 tcctaaaaat tctaaaatgg ggtgcaaatt aagttattat tctgcacata tccaaatttc
     2761 tagctttatt tttttaactt ttaaaaattc cgttgttaat aactcctcta tctgcgtgat
     2821 tattagccga tagaagaaag tgtttttgtt gctgcttgaa cttctgttct tcccggatat
     2881 tgctccaaag ccacttttaa acatgcaata gcattggtta acgactcttt gtttaacaca
     2941 tacgccaaac gaacttgatt cgtgccaatt cctttggttg aataaaaacc tgttgctgga
     3001 gccatcatca ctgtttgacc gttgtattca aaatcttcca acaaccattg acaaaattta
     3061 tc
//
LOCUS       DAOQVQ010000280         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37214_length_3062_cov_3.160625, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000280 DAOQVQ010000000
VERSION     DAOQVQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37214_length_3062_cov_3.160625"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKN22_08370"
     CDS             complement(<1..204)
                     /locus_tag="PKN22_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNS42759.1"
                     /translation="MKVTVVGAGNVGATCADVLAYREIANEVVLLDIKEGFAEGKAMD
                     IWQKAPINLYDTRTVGSTNDYSKT"
     gene            complement(328..2949)
                     /locus_tag="PKN22_08375"
     CDS             complement(328..2949)
                     /locus_tag="PKN22_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS42760.1"
                     /translation="MKKQVYILLLFTIISLVLQVNAQQVPVNCELAIPGCTTPNFTVN
                     GTNPTYDYEDFGTGTITNPSNNPQNGNSGCLLTGETVSTFITISIVNSGTLEWSLIGV
                     NAAGNPTNNGCFDWIMWPNVNNNGCNGIHNNTLPPVACNWNGMCNGNTGMASAANYPP
                     GASSTSYQPPLNVNAGDQFILCLSNFSYTNQNVNLNFFGTAQVTCNPTTPDQTICLGS
                     SATVNILAIGLTNPTYNWLVTTGVSNPTSGSDVIVTPTDTTMYVVEITSIEGVFLDTF
                     YINVVPPPTPNAGPDQILCQGTAIHLSGTLSNAASTYTWGHITTGITPNPTVTYIPNP
                     PSLTPTVVVNQAGTYRFVLTENNGVCPAVKDTVQVLVSKTTHTTSWTGPSCAGMADGT
                     ITITNPDAVEYSFDGGVTWVTNATQGGFAMGTHAVQSRNQYGCIFSSNVTITDPAQLQ
                     INVSNDTLICQNGYADVSAWLGVQGLNEIYHWSATASTAGNVHLGPYTTDQVVTVYAE
                     GQSGCLSDTQTVNITVRQPLAGIISPNDTICPGYPTTVNVAAITGGLAPYTITWSTGD
                     TQTGVTQMEIPANPPQTQVYTVTVNDVCETTPLVLTTQVYVAPLPVPLMSVVEPILCE
                     PAIFEIHNETDTTMMSSYSWEYPYDNTAINEPVIFTDELSAGTYDVALVVVSPLGCID
                     SIRMKDFLTVQPKPVSNFAWSPNPVLEFNTTVYFQDLSELAHEYDWSFPGATPSYSQV
                     ERPKVTYPDGETGTYPVTLIVTSELGCKDTLTQNLVVSPEVTIFIPNTFTPDGDEFNQ
                     SWKIYINGIDLHSFNLQVFNRWGEMVFESLDPSIGWDGTYKGKVVEAGTYVWKISARD
                     RQNDGKYEWNGHVNIIK"
BASE COUNT          909 a          592 c          586 g          975 t
ORIGIN      
        1 tgtttttgaa taatcatttg ttgaacctac tgttcttgtg tcgtacaaat tgattggagc
       61 tttttgccaa atatccatgg ctttgccttc tgcaaaacct tctttgatgt ctaataaaac
      121 tacctcattg gcaatttcgc gataagcaag aacatcagca catgttgcac caacatttcc
      181 cgcacctact accgtaactt tcatgttatt tgatattaaa ttattgaatc tattttaatt
      241 gatgcgaatt taatacaaat gtgtcaaaaa tggtaataaa tcaataaaga aatttagatg
      301 tgttagaaca atcgaaaaac aactgtttta ttttataata ttaacatgcc cgttccactc
      361 gtatttacca tcgttttgtc gatcgcgtgc agaaattttc catacataag ttcctgcttc
      421 taccactttt cctttataag ttccatccca tccaatggaa gggtctaaac tttcaaaaac
      481 catttcaccc caacggttaa atacttgcaa attgaaagag tgcaaatcaa taccgttgat
      541 gtatattttc caactttggt taaactcatc accatctgga gtaaaggtat tggggatgaa
      601 aatcgtaacc tctggactga ctactaaatt ttgagtcaat gtgtctttac aacctaattc
      661 agatgttacg atcaaagtta caggataagt acccgtttct ccatcaggat aagtaacttt
      721 tggtctttca acttgcgaat aggaaggcgt tgcacccggg aaactccaat cgtattcgtg
      781 tgctaattct gataaatctt ggaaatacac cgttgtgttg aattctaaca ctgggttggg
      841 actccacgca aaatttgaaa ccggttttgg ttgaacagtt aagaaatctt tcatgcggat
      901 tgaatctata caacccaaag gcgaaaccac taccaaagcc acatcatagg ttcctgcact
      961 caattcatcg gtgaaaataa caggttcatt aattgcagta ttatcatatg gatattccca
     1021 tgaatagctc gacatcattg tagtgtcagt ttcattgtga atttcaaaaa ttgcaggttc
     1081 acacaatatt ggttcaacca cactcattaa aggtactggc aatggagcca cataaacttg
     1141 agttgttaaa accaaaggag tggtctcaca aacatcatta accgtaactg tataaacttg
     1201 tgtttgaggt gggttggcag gaatttccat ttgtgttact cctgtttgtg tatcacctgt
     1261 gctccaagtt attgtatatg gtgccaaacc tcctgtaatt gctgcaacat taacggttgt
     1321 tggataacca ggacaaatag tgtcgtttgg tgaaataata cccgccaatg gttgtctaac
     1381 agtaatgttt accgtttgag tatcggataa acatccgctt tgtccttctg catatactgt
     1441 tacaacttgg tctgtagtat aaggacctaa gtgtacgtta ccagctgtac ttgcagtagc
     1501 actccaatga taaatttcgt tcaacccttg tacacccaac cacgcactca catctgcata
     1561 accattttga caaatcaagg tgtcgtttga aacattaatt tgtaattgag caggatcggt
     1621 gatggtaacg ttggagctaa atatacaacc gtattgattt cttgattgaa cggcatgcgt
     1681 acccattgca aaaccacctt gtgtagcatt ggttacccac gtaacaccgc cgtcaaagct
     1741 gtattctacc gcatctggat tggttatagt aatggtacca tctgccatac ccgcacatga
     1801 aggaccagtc caactggtgg tgtgggtagt tttagaaaca agtacttgaa cggtgtcttt
     1861 tactgctgga caaacaccat tgttttctgt taatacaaat cggtaagttc ctgcttggtt
     1921 caccactact gttggtgtta aagatggtgg gttgggaatg tatgtaaccg taggattggg
     1981 tgtaatacct gttgtaatgt gtccccatgt ataggttgat gcggcattac tcaatgttcc
     2041 acttaaatga attgcagtac cttgacaaag aatttgatcg ggaccagcgt tgggtgttgg
     2101 aggtggaact acattgatgt aaaatgtatc caaaaacaca ccttcaatgg aagtgatttc
     2161 aacaacatac atggttgtgt ctgttggcgt cacaataaca tcggaaccac tggtagggtt
     2221 agaaacccct gttgtaacca accagttata cgttggattg gttaacccaa tagctaaaat
     2281 attaactgtt gccgaacttc ctaaacaaat ggtttggtcg ggagttgttg gattacacgt
     2341 aacttgagcc gtgccaaaga aatttaaatt aacgttttgg ttggtatagc taaagttgga
     2401 taaacataaa ataaattgat cgcctgcatt cacatttaat ggcggttggt agctagtact
     2461 tgatgcgcct ggaggataat tggcagctga tgccattcct gtatttccat tacacatccc
     2521 attccaatta caagctactg gaggcaatgt gttgttgtgt atgccattgc acccattatt
     2581 gtttacgttg ggccacataa tccagtcaaa acagccatta tttgttgggt ttccagcagc
     2641 atttacgcca attaaagacc attccaaagt accagaatta acaatggaaa ttgtaataaa
     2701 tgtggacacg gtttcacctg ttaataagca acctgaattt ccattttgag gattgttgga
     2761 aggatttgtg attgtcccag ttccaaaatc ttcgtaatca taagttggat tggttccatt
     2821 cactgtaaag ttgggagtag tacaccctgg tattgctaat tcacagttaa ctggaacttg
     2881 ttgtgcatta acttgtaaaa ctagtgaaat aatggtgaat aatagtaata tgtatacttg
     2941 ttttttcatg gcttattcat tgcaccctac aaaaggatat ttattaatcg tatcaactcc
     3001 gtatgttcct tgttgaacac attttatagt atttccataa ataccaaacc aactggacaa
     3061 cg
//
LOCUS       DAOQVQ010000281         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37328_length_3056_cov_3.252249, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000281 DAOQVQ010000000
VERSION     DAOQVQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37328_length_3056_cov_3.252249"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2330)
                     /locus_tag="PKN22_08380"
     CDS             complement(<1..2330)
                     /locus_tag="PKN22_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNS42761.1"
                     /translation="MFTKKIFLFLLSAIPSFAISQTIVSGEVLDSMSNKPIEFASFKL
                     FSQKDSTLKSGAYTDEKGKFLIENIQDGNYYGVLTFSGFEEKTIHNIRIAGQPSYSVG
                     KIKMQTVNIKNIEELVIEGKLETLSAGIDKKVYNVDQDISSKSAGADEVLNNIPSISL
                     DEDGKVSLRGNGSVTILIDGQPSNMTGDGGSFLSSIPANSIERIEVVTNPSARYSPDG
                     TAGIINIVLKKNKLRGFNGLISASGGIPGNDHKLNASLSYRNDKINVFGSYGFNYMDG
                     YRNNYSTMERRLSDTTNMKLNQERVGNHIKRGHSARVGFDWFINNNNTIGLSLNGQFN
                     SENRGGDQLNTQYFTTDVIANQWERKSFEPETRKALDANLYHTFNLKDNSGKLSSSVY
                     YSLSNRVDQGRFEQYYSIFNYATSNANSLYQEQKASNDDGTFSAQIDFEKIVKKINAR
                     YEVGAKASLGSQHLLSNSSSLNILTNQMEPDTFAIYNYKYKEDVYSAYGVFGQELGKF
                     KYQVGLRAEYVLQEPRLLSTAQSFKKEYFQLYPSAHIRYEVAKNAELSLGYSRRINRP
                     KSWDLNPFAIYSDPYNLRMGNPDLKPEFIQSVDLGYTQTFKVISITASVYYRYTNDVI
                     SRIKSFITNNTAAVTMGNISTSQTVGGELVFQIRPTSWWRNTLSFNANYIDYKSKTTA
                     GLNNSGFNWGAKYMGSFDFWKKTASVQINAVYNAPRTTPQGVVYMWNYVDLSVQKSFF
                     NKKFTVGLKLADVFNTKNFRMKLNSGDLSQTSKFHS"
     gene            2462..>3056
                     /locus_tag="PKN22_08385"
     CDS             2462..>3056
                     /locus_tag="PKN22_08385"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNS42762.1"
                     /translation="MGNLISLMTPEQFLYKISTNEQLPGIEAHQLMIPGKRALQRSEV
                     ENLHEYRDSAVAIVCYPIQNQVNCVLIQRPNYEGSHGGQISFPGGKKELTDSSLEYTA
                     RRETFEEIGWQLDSNNFICELTELFIPVSKFSVQPHLYFCEKPQLFVPNQREVDEILH
                     FPVEILLNDAIIKHTDLQASSKIRLKNVPYFEINNRIV"
BASE COUNT          954 a          557 c          520 g         1025 t
ORIGIN      
        1 ttagaatgaa atttggaagt ttgtgacaaa tcaccactgt tcaatttcat tctaaagttt
       61 ttcgtattaa acacatctgc caacttcaaa ccaactgtaa actttttatt aaaaaagctt
      121 ttttgaactg ataaatccac gtaattccac atataaacta caccttgagg agttgttctt
      181 ggtgcgttgt aaacggcatt aatctggaca cttgctgttt ttttccaaaa gtcaaatgat
      241 cccatgtatt ttgctcccca gttaaaacca ctgttattta accctgctgt tgttttgctt
      301 ttgtaatcaa tgtaattggc gttaaacgat aaggtatttc tccaccacga tgttggacga
      361 atttgaaata ccaactcacc tcctactgtt tggctggtgc taatattacc catggttaca
      421 gcagctgtat tgttggtgat aaatgatttg attctactga ttacatcgtt ggtgtaacgg
      481 taataaacac ttgctgtaat agaaattact ttaaatgttt gcgtgtatcc taaatcaacc
      541 gattgaataa actctggttt taaatcagga ttccccattc ttaaattgta agggtcggaa
      601 taaattgcaa atggattcaa atcccacgat tttggtcgat tgattcttct ggaatatccc
      661 aaacttaatt ctgcattttt agccacttca taacgtatgt gcgcagaagg atacaattga
      721 aaatattctt ttttaaacga ttgagctgtg gaaagcaagc gcggttcttg caatacatat
      781 tcggcacgca atcctacttg gtacttaaat ttacccaatt cttgtccaaa aacaccataa
      841 gcactataca catcttcttt gtacttgtaa ttgtaaattg caaaagtatc cggttccatt
      901 tgattggtaa gaatgttcaa actggatgaa ttggagagta aatgttgact tcctaacgat
      961 gcttttgctc ccacttcata gcgggcattg attttcttaa ctattttttc aaaatctatt
     1021 tgagcagaaa aagttccgtc gtcattgctt gctttttgtt cttgatacaa tgaatttgca
     1081 ttggaagtag cataattgaa aatagaataa tactgttcaa aacgtccttg atctactctg
     1141 ttggatagcg agtaataaac cgaagacgaa agtttccctg aattatcttt tagattaaac
     1201 gtatgataca aattggcatc caacgctttt cttgtttcgg gctcaaacga ttttctttcc
     1261 cattgattgg caataacatc tgtggtgaaa tattgggtat ttagttggtc tcctcctcta
     1321 ttttcagaat taaattgtcc gttgagtgaa agcccaattg tattgttgtt attaataaac
     1381 caatcaaaac caacacgtgc agagtgacct ctttttatgt gatttccaac tctttcttga
     1441 ttcaatttca tatttgtagt gtcagaaagg cggcgttcca tggtgctata attattgcgg
     1501 tagccatcca tataattgaa tccatacgaa ccaaaaacat tgattttatc gttgcgatag
     1561 ctcaaagaag cattgagttt gtgatcgttt cctggaatac cacccgaagc agaaatcaag
     1621 ccattaaaac cgcgtaactt attctttttc aatacaatgt tgataatacc cgcagttccg
     1681 tcaggactat aacgtgccga tggattggta acaacttcaa tgcgttcaat ggaattggca
     1741 ggaatggaac tcaaaaaact tccaccatca ccagtcatgt tgctaggttg tccatcaatt
     1801 aaaatcgtta cgcttccgtt tcctctcaaa gaaaccttac cgtcttcatc caatgaaata
     1861 gaaggaatgt tgtttaaaac ttcatcagct ccagcacttt tggaagaaat atcttgatcc
     1921 acattgtaca cttttttgtc aattccagcg ctcaatgttt ccagtttacc ttcaatcacc
     1981 aattcttcaa tgtttttgat gttgacagtt tgcattttta tttttccgac agaataagaa
     2041 ggttgacccg caatgcgaat gttgtgtatt gttttttctt caaatccaga aaaagtaagc
     2101 acgccataat agttgccatc ttgaatgttt tcaatcaaaa attttccttt ttcatccgta
     2161 taggcgccac ttttaagtgt agaatctttt tgagaaaata atttgaatga tgcaaattcg
     2221 ataggtttat tgctcatgct atccaaaact tcgccagata caatggtttg agaaattgca
     2281 aaagatggaa ttgcacttaa gagaaataag aagatttttt tagtaaacat taatataaag
     2341 tgttgtgttc tgtaaacgaa caaatttaag cgataatgcc taaacaaaag catttataac
     2401 aatttaataa gtttttttaa caaacagaag tgtttcaatc taccgcaaaa tatgattatt
     2461 tttggggaac ttaatttcac tcatgacacc cgagcaattt ttatataaaa tttcaactaa
     2521 cgagcaatta cccggcattg aagcccatca attgatgatt ccaggtaaaa gagcgttgca
     2581 acgttctgaa gtggaaaatt tgcacgaata ccgtgattct gctgttgcaa tagtgtgtta
     2641 tcctattcaa aatcaggtga attgtgtttt aattcaacga cccaactatg aaggaagtca
     2701 tggcggacaa attagttttc cgggaggaaa aaaagaatta acagatagtt cattagaata
     2761 tacagctcga agagaaactt ttgaagaaat tggttggcaa ttggatagca acaatttcat
     2821 ttgtgagttg accgaattat ttattcccgt aagcaaattt tcggtgcagc cccacctcta
     2881 tttttgtgaa aaaccacaac tttttgttcc taaccaacgc gaagtggatg aaatacttca
     2941 ttttccagta gagattttat tgaacgatgc aatcatcaag cataccgatt tgcaagcaag
     3001 tagtaaaatt cgactgaaaa atgtccccta ttttgagatt aacaaccgaa ttgttt
//
LOCUS       DAOQVQ010000282         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37533_length_3045_cov_4.055518, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000282 DAOQVQ010000000
VERSION     DAOQVQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37533_length_3045_cov_4.055518"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="PKN22_08390"
     CDS             complement(<1..1486)
                     /locus_tag="PKN22_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNS42763.1"
                     /translation="MRIFNLVLATIALAFSCFSQKDISVESIYKTYQFYPKGVQGFNG
                     MKDGMSFTQISETDQGDAITKHKITAYQDKGEVLLSLSDLKYNGEAIEVDNYSFNDDE
                     TKLLLATQTTSIYRRSYTAVFYIYDLTTKKLEILDTQHQPQTLATFSPDGSKIAYISD
                     NNLYYKDLKTQQIETITTDGKFNRIINGTTDWVYEEEFGITQAFGWSPDSKNIAFLRF
                     DESNVKEFTMEYNIGELYPELYTFKYPKAGEDNSKVTAHIFSLNNKQTVGVQLGAYEY
                     IPRISWSNINNTLILQTLNRHQNKVDYYKVEQQANTWVATSFYTEQNNTYIDIDDNLI
                     FLNSGTAILRTSEKDGYKHIYQLGFDGKETQITKGNWDVIDFCGIDTKNKFVYYTAAK
                     KGAIHQGIYKVDLKSKKDLAISLELGQNSADFTPGMNYFIKSYSNANTPPIYTLCDNK
                     GKELTVMEDNNELVEKIQQHHFAKKEFVTFDMGAYTLNGWIIKPS"
     gene            complement(1518..2315)
                     /locus_tag="PKN22_08395"
     CDS             complement(1518..2315)
                     /locus_tag="PKN22_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42764.1"
                     /translation="MAKDIFDSILEKEFHQLTPQELTELNDYCQTATEFDAFKKVLQH
                     TRTLANAPKITPLAATKDKLDDLFASTHAPKGILFYQTPIFKIAASLLLLIGVGIGFY
                     YINSTPQKVQLAENTQKETPAQEKNEIAPPTKAIKATTDSSTATEKSTATKKTPEDKQ
                     LIAPATAASPVQLMESSINEESLTRLSQDAISSMDNDKMDANTQKAKAEMPPPVSEKA
                     HPTFSTPATSVQDIQVISTKRAQNKSVATSVNLAQQNNVLKFLVVKY"
     gene            complement(2308..2907)
                     /locus_tag="PKN22_08400"
     CDS             complement(2308..2907)
                     /locus_tag="PKN22_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS42765.1"
                     /translation="MRLFKKAYDKCSDEELMKRLTSGEKQAFDVLYTRYATLLLNYFF
                     RMLWKDREKAEDFVHDLFAKIIQNPTSFDTTRNFKTWIYSVANNMCKNEYKKQAVRAG
                     TKNGLEDYMAVPEETDGVFKQTHFTLFKNEFEEKLNQLDDKHREAFIFRHIEGLSIKE
                     IADLLQINEGTIKSRIFYATKQLAEELAHYQPEKSKHHG"
BASE COUNT          807 a          500 c          580 g         1158 t
ORIGIN      
        1 tgcttggttt aattatccat ccgttcaaag tataggcacc catatcaaag gttacaaatt
       61 ctttttttgc aaaatggtgt tgttgaattt tttctactag ttcattgttg tcttccatga
      121 cagtcaattc cttgcctttg ttatcgcaaa gtgtatagat tggtggcgta ttggcgttgc
      181 tgtagctttt aatgaaatag ttcatgcctg gtgtaaaatc ggcactgttt tgtcctagtt
      241 ctaagctaat ggcaaggtct tttttacttt tcaaatccac tttgtaaatt ccttggtgaa
      301 ttgccccttt tttagccgcg gtgtagtaca caaatttgtt tttggtatcg atgccacaaa
      361 aatcaatcac atcccaattt cctttagtga tttgggtttc ttttccatca aaacctaatt
      421 gataaatgtg tttgtacccg tctttttcac ttgtgcgcaa aattgctgtt ccactgttga
      481 ggaaaatcaa attgtcatca atgtcaatgt aggtgttgtt ttgctcggta taaaaagatg
      541 ttgccaccca tgtgtttgct tgttgctcca ctttgtaata atcaaccttg ttttggtgac
      601 gatttaatgt ctgcaaaatc aatgtgttgt tgatgttgga ccaagaaata cgtggaatgt
      661 attcgtaagc acccaattgc actccaactg tttgtttgtt gttcaacgaa aaaatgtggg
      721 cggtaacttt ggagttatct tcgcctgctt tgggatattt aaaagtgtac aattctggat
      781 acaattcacc aatgttgtat tccattgtaa attctttaac atttgactcg tcaaaacgca
      841 agaaagcaat gtttttactg tcgggcgacc aaccaaaggc ttgagtaatg ccaaattctt
      901 cttcatatac ccaatccgtt gttccattta tgatgcgatt gaattttcca tccgttgtaa
      961 ttgtctcaat ttgttgagtt tttaaatctt tgtaatacaa attattgtca ctaatgtagg
     1021 cgattttact tccgtctggt gagaatgtag ccaaggtttg cggctgatgt tgggtatcta
     1081 gaatttctaa ttttttagtt gttaaatcat aaatataaaa aaccgccgta tagcttcttc
     1141 tgtaaatgct tgttgtttga gttgccaata ataatttggt ttcatcgtcg ttaaacgagt
     1201 aattatcaac ttcaattgct tcaccgttgt attttaaatc gcttaacgaa agcaacactt
     1261 cgcctttatc ttggtatgct gtgattttat gtttggttat ggcatctcct tgatcggttt
     1321 cagaaatttg agtaaacgac ataccgtctt tcattccatt aaaaccttga actcctttag
     1381 gataaaattg gtaggttttg taaatagatt ctacagaaat gtctttttgg ctaaagcaag
     1441 aaaatgcaag agctattgta gctagaacaa ggttgaaaat acgcatacat taaaaatttg
     1501 atgagctaaa aatacaatta atattttaca acaaggaatt taagcacgtt gttttgttgc
     1561 gccaaattaa cagatgttgc aacgctttta ttttgagcac gttttgtaga aattacttga
     1621 atgtcttgaa cagatgttgc aggtgtgcta aaagttggat gtgctttttc gctaacagga
     1681 ggaggcattt ctgcttttgc tttttgggtg tttgcatcca ttttgtcatt atccattgaa
     1741 ctaattgcat cttgggacaa tcgtgttagt gattcttcgt tgatggaaga ttccattaat
     1801 tggacaggtg acgcagcagt tgcaggagcg attaattgtt tgtcctcggg tgtttttttt
     1861 gttgcagtgg atttttctgt ggcagtagat gaatctgttg ttgctttaat tgcttttgtt
     1921 ggaggagcta tttcattttt ttcctgagct ggtgtttctt tttgtgtatt ttctgctaat
     1981 tgcacttttt gaggagtaga attgatgtaa taaaatccaa ttccaacgcc aattagcaat
     2041 aggagagaag ctgcaatttt aaaaattggt gtttggtaaa acaagatgcc ttttggtgcg
     2101 tgtgtgcttg caaacaaatc atccaattta tcttttgttg ctgcaagcgg agtaattttg
     2161 ggtgcatttg caagtgtgcg tgtgtgttgc aatacttttt tgaatgcatc aaattctgtt
     2221 gcagtttggc aataatcgtt cagctcagtt aattcttgag gtgttaactg gtgaaattct
     2281 ttttccaaaa tactatcaaa aatatcttta gccatgatgc tttgattttt caggttgata
     2341 atgtgctaat tcttctgcca attgtttggt agcataaaaa atgcgcgatt tgatggttcc
     2401 ctcgttgatt tgcaataaat cagcaatttc tttgatggac aatccctcga tgtgcctaaa
     2461 aataaatgct tcgcgatgtt tatcgtccag ttgatttaat ttttcctcaa attcattttt
     2521 aaacaatgta aaatgcgttt gtttgaacac gccatccgtt tcttcgggca cagccatata
     2581 gtcttccaaa ccgtttttgg ttcccgctct tactgcttgc tttttatatt catttttaca
     2641 catgttgttg gctaccgaat aaatccaagt tttaaaattg cgtgttgtat caaatgaggt
     2701 ggggttttga ataatttttg caaacaaatc gtgtacaaaa tcttcggctt tttcacggtc
     2761 tttccacaac attcgaaaga aataattgag caacaatgtg gcataacggg tgtataaaac
     2821 atcaaatgct tgtttttctc cagaagttaa tcgtttcatt aattcttcat cagaacattt
     2881 gtcgtatgcc tttttgaaaa gcctcattct tcgattccta aatcattgat taggtgattg
     2941 tttttgatta aagttccaac aaatttgatt ccgtttttta tttctgtagc catttttttg
     3001 ttattggaag tggtgtaaaa ttcatgcaag ttcaataaca atggc
//
LOCUS       DAOQVQ010000283         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37570_length_3043_cov_3.522423, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000283 DAOQVQ010000000
VERSION     DAOQVQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37570_length_3043_cov_3.522423"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKN22_08405"
     CDS             complement(<1..799)
                     /locus_tag="PKN22_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42766.1"
                     /translation="MKAFFQKNWIHFAAIAIMYIVTLIYCKPVTEGYGIKQHDIVQYH
                     GMAHETNEFRKLFDKEALWTNSMFAGMPTMQISVIYSGNWIGKVLNTYYTTTFGRPVD
                     IIFMHMLGFYIFAMLLGMNPLVGLFGAIAMAFASYEIIVIQAGHNSKAMATAFMAPVL
                     GAFIRFYKDKKLSLWMLALAGLFMAFELASNHVQVTYYLAFLLVGLGIYFLVKALKEK
                     ELKSFIIKSAALISVFLLAALINSGNLILTNDYAKHTIRGKNDVTINP"
     gene            complement(812..1990)
                     /locus_tag="PKN22_08410"
     CDS             complement(812..1990)
                     /locus_tag="PKN22_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS42767.1"
                     /translation="MSKILIIDDERAIRRALREILEFENFEVDEAEDGKQGVEKATSE
                     VFDIIFCDIKMPNMDGMEVLDALQEASVETPVIMISGHGTIETAVQALKKGAFDFIEK
                     PLDLNRILVTVRNASDKTALVEEKKVLKKTVQRFKGTSIIGESAEIMQIKAMIEKVAP
                     SDARVLITGGNGTGKELVARSLHDQSNRKDFPFIEVNCAAIPSELIESELFGHEKGAF
                     TSAIKQKKGKFELAEGGTLFLDEIGDMSASAQAKVLRALQENTIQRVGGEKDIKINTR
                     VVAATNKDLRKEIEEGRFREDLYHRLAVILIHVPSLNDRRDDIPVLAEHFLTMVCGEH
                     GIQRKNFSKDALEALKNTDWTGNIRELRNIVERLVILCDNTISAEDVKLFANPKAIKN
                     "
     gene            complement(2053..2718)
                     /locus_tag="PKN22_08415"
     CDS             complement(2053..2718)
                     /locus_tag="PKN22_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42768.1"
                     /translation="MMNRAFLLLFLGFSMHFTTHSQVFYFNNTSTTLVKTVNQSPAHW
                     YIEIFSNSATDVTLKWKAIFSQIPPQWVINFDDQTVNHTPVNDGDEANFTLFPTEEFP
                     QKLIIGATLNNTPGIGTAAFEISDPNTNYKDTIHYIFHVTDNLSVEKSDITLPYSIKN
                     GVLTIENDLENSVLIYDESGRVIDELNNCNSYTIKDLKKNTLYYIQLYCKQKVYSIKY
                     FNN"
     gene            2804..>3043
                     /locus_tag="PKN22_08420"
     CDS             2804..>3043
                     /locus_tag="PKN22_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNS42769.1"
                     /translation="MQSNTNYLENINFPSDLRGQFTIDDLPAISAEIRQYIIDVISEI
                     GNSHFSSSLGAVELAVALHFVYNTPTDQIIWDTGHQ"
BASE COUNT          955 a          574 c          487 g         1027 t
ORIGIN      
        1 ctggattgat ggttacatcg tttttaccac gaatggtatg tttggcataa tcgtttgtaa
       61 gaataaggtt gccagagtta atcaaagcag ctaataaaaa gacacttatt aatgcagctg
      121 atttaataat aaaacttttt aactcctttt ctttcaatgc tttcacaagg aaatagatac
      181 ccaatcctac caataaaaat gctaaataat aggttacttg aacatggtta ctggcgagct
      241 caaaggccat aaacaatcct gcaagagcca acatccacaa cgatagtttt ttatctttat
      301 aaaaacgaat gaatgcacct aacactggtg ccataaatgc cgttgccatt gcttttgaat
      361 tgtgcccagc ttgaataaca atgatttcat aacttgcgaa tgccattgca attgcgccaa
      421 acaaaccaac caaaggattc attcccaaca acattgcaaa aatgtaaaaa cctagcatgt
      481 gcataaaaat gatgtccaca ggtctgccaa aagtagttgt gtaataagta tttaacactt
      541 ttcctatcca gtttccagag tagataaccg aaatctgcat ggttggcatc cctgcaaaca
      601 tagagttagt ccacaaagct tctttatcaa ataattttct aaattcattt gtttcatgag
      661 ccattccatg atattgaaca atatcgtgtt gtttgatgcc atatccttct gtaacaggtt
      721 tgcaataaat gagcgtaaca atatacataa tggcaattgc cgcaaaatgg atccagtttt
      781 tttgaaaaaa tgctttcata gtattcaagt cttaattttt tatagcttta ggatttgcaa
      841 ataattttac gtcttcggca ctgatagtgt tgtcgcataa aataaccaaa cgttccacaa
      901 tattgcgcaa ctcgcgaata ttccctgtcc aatcggtgtt ttttaatgct tccaatgcat
      961 cttttgaaaa gtttttacgc tgaattccgt gctcaccaca taccatcgtt aaaaaatgct
     1021 ctgctaaaac aggaatatcg tctcgtctat cgttgagtga tggaacgtga attaaaataa
     1081 ccgccaaacg gtggtataaa tcttctctaa aacgtccttc ttcaatttct ttacgtaaat
     1141 ctttgttggt tgcagcaaca acacgggtgt taattttaat atctttttcg ccacctacac
     1201 gctgaatggt gttttcttgt aatgcacgca atacttttgc ttgtgccgat gccgacatat
     1261 caccaatttc atctaaaaac aaagtaccgc cttcggccaa ttcaaatttt cctttctttt
     1321 gttttatggc agaggtaaag gctccttttt catgaccaaa taattcgctt tcaatcaatt
     1381 cggaaggaat tgcagcgcag tttacttcga taaatggaaa atctttacgg ttggattgat
     1441 cgtgcaatga acgcgccacc aattcttttc cagtaccatt tccacctgtt atcaaaacac
     1501 gagcatcgga tggagcaact ttttcaatca ttgccttgat ttgcataatt tcagccgatt
     1561 ctccaatgat agatgtgcct ttaaaacgtt gaacggtttt ctttagtact tttttctctt
     1621 caaccaaggc tgttttatca gaagcattgc gaactgttac caaaatacgg ttcaaatcca
     1681 acggtttttc aataaaatca aaagcacctt ttttcaatgc ttgaacagct gtttcaatgg
     1741 ttccgtgccc actaatcata ataaccggcg tttcaacaga agcttcttgg agtgcatcca
     1801 aaacttccat tccatccatg ttgggcattt ttatatcgca aaaaatgatg tcaaaaactt
     1861 cagatgttgc tttttcaact ccttgtttgc catcttcggc ttcatcaact tcaaaatttt
     1921 caaattctaa aatttcacgc aatgctcttc gaattgcgcg ttcatcatca atgattaata
     1981 ttttgctcat ttaactttgt ttggtgtaaa tataataaga agcaatttat tccaattatt
     2041 cttcctaaaa aactaattat tgaaatactt aattgaatac actttttgtt tgcaatacaa
     2101 ttgaatgtaa tacaatgtat ttttctttaa gtctttaatt gtgtaggaat tacaattatt
     2161 taattcatca ataactctac ccgattcatc gtaaattaaa acactatttt ccaggtcatt
     2221 ttctattgtc aaaaccccat ttttaatcga ataaggcaat gtaatatctg atttttctac
     2281 cgataaatta tccgttacat gaaaaatata gtgaattgta tctttgtaat tggtgttggg
     2341 gtcagaaatt tcaaatgcag ccgtaccaat tccaggtgta ttgttcaagg ttgcgccaat
     2401 aattaatttt tgcggaaatt cttctgtggg aaacaaggtg aaattagctt catctccatc
     2461 gtttacaggt gtatgattaa ccgtttggtc atcaaaattt ataacccatt gcggtggaat
     2521 ttgagaaaaa atagctttcc attttagggt aacatctgta gcagaattgg agaaaatttc
     2581 aatgtaccaa tgagcaggcg attggttaac cgttttcacc aaagttgttg atgtattgtt
     2641 gaaataaaac acttgtgaat gggtagtaaa atgcattgaa aaccccaaaa acaatagtaa
     2701 aaaagcacga ttcatcatac cgatttaaaa taaatttgtc taaagataag ttatttttgc
     2761 ttttataggt gattagcact aattttgtgc aaattttata gcgttgcaaa gcaataccaa
     2821 ttatctagaa aacatcaact ttccttctga tttaagaggg caatttacta ttgatgactt
     2881 accagctatt tcggccgaaa ttcgtcaata cattattgat gtgatttcgg aaattggcaa
     2941 cagtcatttt agctctagtt tgggggctgt tgaactagct gttgcgcttc attttgtcta
     3001 taacacacct acagatcaaa tcatttggga tactggacat cag
//
LOCUS       DAOQVQ010000284         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37834_length_3028_cov_3.070972, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000284 DAOQVQ010000000
VERSION     DAOQVQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37834_length_3028_cov_3.070972"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="rnpB"
                     /locus_tag="PKN22_08425"
     ncRNA           complement(<1..305)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN22_08425"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(322..1404)
                     /locus_tag="PKN22_08430"
     CDS             complement(322..1404)
                     /locus_tag="PKN22_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42770.1"
                     /translation="MKTIALTLFLAVNFYFVTGQITIEDTDLPVAKDTLVSSQAVNFA
                     IDYASTGPDYQWDFSSLSFLSQITRIYQPLSGVPILINMAYGPFAVTKYRSSYYMPYM
                     DLPLGQIAQILPINFDLDDVNQYTRKTTTRLNLVGYSIGISGQGVPMKSDTIETKYVL
                     PLNYGDEYFSTGYTNFDLSQFIDAQWIQKRKRHTVVDGWGKITTPYGTFDVLRVQHRV
                     EETDTIGYNGTVFGLEIPVVYEYEWLAKNEKTPILRITTSEIMGNETVVSIEYKDLDY
                     LSTTTPKAPNQLAIYPNPASDELIVSWQEGMKNCTIHQLDGTLVKQFSFASTTQSIAV
                     DDLTPGMYLIQVECNGQSARMPFVKK"
     gene            complement(1459..2184)
                     /locus_tag="PKN22_08435"
     CDS             complement(1459..2184)
                     /locus_tag="PKN22_08435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNS42771.1"
                     /translation="MLIKNLHLDKVLFLDIETAPQVYLPQYLDDESRKLFGDKAHVSP
                     DDIGDFEAAYAKRASIFAEFGKIICISVGFVTETRTGRQMRLKSFYHDDEETLLLQFK
                     KLIEEHYNTQYHLLCGHNAKEFDFPYICRRMLVNGIEIPAVLNLAGKKPWEVMHLDTM
                     ELWKFGDYKAYTSLALLCHIFGIPTPKDDISGADVARVYYEDKDLERIKVYCEKDVVA
                     LIQLFLKMQGLPLIDEGEIYHSN"
     gene            complement(2270..>3028)
                     /locus_tag="PKN22_08440"
     CDS             complement(2270..>3028)
                     /locus_tag="PKN22_08440"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS42772.1"
                     /translation="QVVVTVNQLPLVNAGVDVTICTGESATITASGAATYSWDNGLGA
                     GSSHTTSPPITTVYTVTGTDIYGCVNTDQMTVFVNEMPSAAIAFVDSTIGCPTVVNFS
                     GTNSLNTASYQWTFPGGTPSTSNSASPSISFDEAGSITITLVTSNGCGTATQNFTFEV
                     AVNCAGLEELTNNFAITIDEAQKNILVFSSSGFDKDARVQLFNELGQTLTAKAIEANQ
                     TAVTIPIDNLAAGVYMVHIKNASFEKLFKVVKQH"
BASE COUNT          943 a          605 c          508 g          972 t
ORIGIN      
        1 tgtacgatct atcaacctac cctctgagtt gggcgagtaa ccctttcatt ttatattgct
       61 acaaaacatc cccagtttac atggtctttc aacgcgtaag gtttacctag ctacaaaaca
      121 tcactgcttg cacggtgagc tcttacctca ccttttcact atgaccccga agggctacct
      181 attttctgct gcactttctg ttgccgaaaa aatcgacacc ttcccgttag gaagtacgct
      241 actctatgtt gcccggactt tcctcttttt cgattgctcg aaacagcgat aaaccgattt
      301 gcctgttaca aaattataaa attatttctt cacaaacggc attcttgctg attgtccgtt
      361 gcattcaact tggataaggt acatcccagg cgttaaatca tctacggcaa tcgattgtgt
      421 ggtagaggca aacgaaaatt gttttacaag cgttccatcc aattggtgaa tggtgcaatt
      481 tttcattcct tcttgccaag aaacaattaa ttcatcgcta gcagggttgg gataaatagc
      541 caattgatta ggagcttttg gagtagttgt acttagataa tccaaatctt tgtattcaat
      601 ggaaacaact gtttcatttc ccataatttc tgaggttgta atgcgaagaa tgggcgtttt
      661 ttcgtttttt gccaaccatt catattcgta aacaactgga atttctaatc caaaaacagt
      721 tccgttataa ccaatggtat ctgtttcttc tacgcgatgt tgaacacgca atacatcaaa
      781 agttccataa ggcgttgtga tttttcccca accatcaacc actgtatggc gttttctttt
      841 ttgaatccat tgtgcatcaa taaattgact caaatcaaaa ttggtatatc ctgtagaaaa
      901 atattcatca ccataattga ggggcaacac gtattttgtt tcaatagtat ctgatttcat
      961 gggtactccc tgaccgctaa taccaataga ataacccact aaatttaagc gcgttgttgt
     1021 ttttcgagtg tattgattca catcgtctaa atcaaaatta atgggtaata tttgtgcaat
     1081 ttgtcccaac ggtaaatcca tatacggcat gtaataagac gaacggtatt tggtaactgc
     1141 aaaaggtcca tacgccatat taatcaaaat tggaacacca gaaagtggtt ggtatattct
     1201 tgtaatttga cttaaaaacg aaagggaaga aaaatcccat tgatagtcgg gacctgtaga
     1261 ggcataatca attgcaaaat taacagcttg actagacacc aaagtatctt ttgctaccgg
     1321 taaatcggta tcttctatgg taatttgacc tgttacaaag taaaagttaa cagccaaaaa
     1381 taaagttaat gcaattgttt tcataaagtt aaataaaaca cgaagataca ttttatattc
     1441 attcaatcaa aaaaaacatt aattggaatg ataaatttct ccttcatcaa tcaaaggaag
     1501 tccttgcatt tttaaaaaca gttgtatgag tgccaccaca tctttttcgc aatacacttt
     1561 gatgcgctct aaatctttgt cttcgtaata cacgcgtgcc acatctgcac cagaaatatc
     1621 atcttttggt gttggtattc caaaaatatg acacagcaaa gccaatgatg tgtatgcttt
     1681 gtaatctcca aacttccaca attccatcgt atccaaatgc attacttccc acggtttttt
     1741 acctgccaaa tttagcactg caggaatttc aattccattc accaacatac gtcggcagat
     1801 gtaagggaaa tcaaattctt tggcgttgtg accacacaac aaatggtact gtgtgttgta
     1861 atgttcttca atcaactttt tgaattgcaa caacaacgtt tcttcgtcgt cgtggtaaaa
     1921 ggattttaaa cgcatttgtc gcccagtacg cgtttctgtt acaaacccaa cagaaatgca
     1981 gataattttc ccaaactcag caaatatact tgctcttttg gcataagcgg cttcaaaatc
     2041 accaatatca tcgggtgaaa cgtgtgcttt gtctccaaat aattttcttg attcgtcgtc
     2101 taaatactgt ggaaggtaaa cttgtggagc tgtttctatg tctaaaaaca atactttgtc
     2161 gaggtgtaaa ttttttataa gcataaatct gtattttcct atctaagtta ataaaaaatg
     2221 tcttcccaaa acaaattgag aagacatttt ttgaatttta aaagaagatt taatgttgtt
     2281 ttacaacttt aaataatttt tcaaaagaag cattttttat gtgcaccata taaacacctg
     2341 ctgcaaggtt gtctattgga attgtaactg ctgtttgatt ggcttcaatt gcttttgcag
     2401 ttaaagtttg tcctaattca ttaaacaatt gaacacgtgc gtctttgtca aaacctgaag
     2461 atgaaaacac caatatattt ttttgtgctt catcaattgt aattgcaaag ttattggtta
     2521 actcctccaa tccagcacaa tttacagcca cttcaaaagt aaaattctgc gtagccgttc
     2581 cacagccatt agaagttacc aacgtaatgg taatgcttcc tgcttcgtca aaagaaattg
     2641 atggagaagc actgttagat gtacttggcg taccacctgg gaaagtccat tgataactag
     2701 ctgtattcaa tgagttggtt ccactaaaat taacaactgt tggacaacca atagtgctat
     2761 ccacaaaagc aattgcagca cttggcattt cgttcacaaa aacagtcatt tggtcggtat
     2821 taacacaacc atatatatct gtaccagtta ccgtataaac agttgtaata ggaggcgaag
     2881 tggtatgcga tgatcctgca cctaaaccat tatcccaaga ataagtagcc gctcctgatg
     2941 ctgtgattgt tgcgctttca cctgtacaaa tagttacatc aactccagca ttaacaagcg
     3001 gtaattgatt tacagtaacc actacttg
//
LOCUS       DAOQVQ010000285         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_37873_length_3026_cov_3.545271, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000285 DAOQVQ010000000
VERSION     DAOQVQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37873_length_3026_cov_3.545271"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..681)
                     /locus_tag="PKN22_08445"
     CDS             complement(151..681)
                     /locus_tag="PKN22_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNS42773.1"
                     /translation="MATTKWTIDPTHSEVSFKVKHLMISNVTGYFGEFQGGALTDEND
                     FSTAQFEFSAPIASLSTNNEQRDGHLKNADFFDMEKYPALTFKSKKVEKIDDETFTIL
                     GEFTIKDVTKEAKLNMELGGITKDPYGNTKAGLTLSGKINRTDYGLTYNAVMETGGVM
                     LSEEVRLNAEIQLVKM"
     gene            828..1961
                     /locus_tag="PKN22_08450"
     CDS             828..1961
                     /locus_tag="PKN22_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNS42774.1"
                     /translation="MHLKSLQIINFKNYEEAYLELNPGINCFIGMNGAGKTNILDAVH
                     YLSMCKSYLNTIDRQNIRFDQQFFSIIGGFETDEKNYEIHCALKVGAKKVFKKNKKEY
                     EKLAEHIGLFPTVIISPYDKDLISEGSELRRKWMDGIISQSDKGYLNDIQKYAKILVQ
                     RNALLKHMYENGFFERESMEVWNEQLVAVGKRIFQKRTDFLNDFIPVFQQHYNSIGNE
                     NELVALEYRSQLHEDSFENLLKNSERKDALSHYTNVGTHKDDLNFLIKGHPVKKFGSQ
                     GQQKSFVIALRLAQYDWLKRNLQLKPILLLDDVFDKLDRNRVQRLMDLVSSDFFGQVL
                     VTDTDEERIRQTFESNQLNGNVYKIEQGNVIPLPQLQVKYQNV"
     gene            1958..2260
                     /locus_tag="PKN22_08455"
     CDS             1958..2260
                     /locus_tag="PKN22_08455"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNS42775.1"
                     /translation="MTMDKRDAENTPLKDLIDRFLKSYRLDKKMKEMEVINGWKEMMG
                     VAVANRTERLYIKNKVLHIKLNSAVMREELANGKQIILLRVNEFAGFEIINDIWFE"
     gene            complement(2261..2815)
                     /locus_tag="PKN22_08460"
     CDS             complement(2261..2815)
                     /locus_tag="PKN22_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNS42776.1"
                     /translation="MKVTFKTAIICALVWIGFKMLSFGVGWNVPSNTALFVMTNMLLL
                     LAAVAIGLYLQKRKGVEDDNILTDIKNGMSAGVVYSMIISAFLYFYYEKIDPDYNRKM
                     VAEREAYFQSVIDNPVELKKIKANNKDLEIMSREEILQEFRKTPQMLFSGGFIMTYGI
                     LSMLMLSTFYSVIVAIIFRKVLFR"
     gene            complement(2820..>3026)
                     /locus_tag="PKN22_08465"
     CDS             complement(2820..>3026)
                     /locus_tag="PKN22_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNS42777.1"
                     /translation="NRRIYPAIDITASGTRREDLLVTKDVLQRVWLMRKFIADMNPVE
                     AMEFMKERMDATQSNEEFLVTMNS"
BASE COUNT         1019 a          529 c          499 g          979 t
ORIGIN      
        1 aagaatagct gtaacagcaa ataatacgta ttttttcatt ctattttatt aaaaaacgat
       61 acaaatttcg ttaattttca ttgtaaacca aaaaaaaatg gatgaaaagc aaaatttcca
      121 tccattctaa taatcaataa ttagttaata ttacatctta accaattgaa tttctgcatt
      181 taagcgcact tcttctgaga gcattacacc tcctgtttcc attactgcgt tgtaggtcaa
      241 accataatct gtacggttga tttttccact caatgttaaa ccagcttttg tatttccgta
      301 tggatctttg gttataccac ccaattccat gtttaatttt gcttctttag ttacatcttt
      361 aatcgtaaat tcacctaaaa ttgtaaacgt ttcatcgtca attttttcca cttttttact
      421 tttaaaagtt aaagcagggt atttttccat atcaaagaaa tcagcatttt ttaaatgtcc
      481 gtcacgttgt tcgttgttgg ttgaaagact tgcaattgga gcagaaaatt caaattgtgc
      541 agtagaaaaa tcattttcat ctgttagtgc ccctccttga aattctccaa aataacctgt
      601 tacattggaa atcattaaat gtttcacttt gaaagaaact tcgctatgtg ttggatcaat
      661 tgtccatttt gtagttgcca tattattctg tttttaaaat tatactgcaa aattacatcc
      721 ttgttaccac ctgtaaattg acctatgata agaaatgaat tgggtgttaa aaattttaat
      781 tcatcgcttt taaaaatcat cacactagtt ttactatttt tgtttttatg catctcaaat
      841 cacttcaaat tatcaatttt aaaaattacg aggaagctta tcttgagtta aatcctggta
      901 ttaattgctt tattggaatg aatggagccg gtaaaaccaa tattttggat gccgtgcact
      961 atttgagtat gtgtaaatcg tatttaaaca ccattgaccg tcaaaacatt cgatttgacc
     1021 aacagttttt ttccatcata ggaggatttg aaacggatga aaagaattac gaaattcact
     1081 gtgcattaaa agttggtgca aaaaaagttt ttaaaaaaaa taaaaaagaa tacgagaaat
     1141 tggccgaaca cattggactt tttcctaccg ttatcatttc tccctacgat aaagatttaa
     1201 tttcggaagg aagtgaattg cgcagaaaat ggatggatgg catcatttcg cagtccgata
     1261 aaggctactt gaacgatatt caaaaatacg ccaagatttt agtgcaacgc aacgccttgt
     1321 tgaaacacat gtatgaaaat ggattttttg aacgtgaatc aatggaggtg tggaacgaac
     1381 aattagttgc tgttggaaag cgtatttttc aaaaacgcac cgattttctc aatgatttta
     1441 ttcctgtttt tcagcaacat tacaacagta ttggcaatga aaatgaattg gtagcattgg
     1501 aataccgttc gcaattgcac gaagattctt ttgaaaattt acttaaaaac agcgaacgaa
     1561 aagatgcttt atcgcattac accaatgtag gcacgcacaa agacgatttg aattttttga
     1621 ttaaaggaca tccggttaag aaatttggtt cgcaaggcca acaaaaatcg tttgttattg
     1681 cgcttcgctt ggcgcaatac gattggttga aacgcaattt gcaactaaaa cccattttgt
     1741 tgctcgatga tgtgtttgat aaattggaca gaaaccgagt gcaacgcttg atggatttag
     1801 tttcatccga tttttttggt caggtattag taaccgacac cgatgaagaa agaatccgac
     1861 aaacgtttga gagcaatcaa ctcaacggaa atgtctataa aatagaacag ggaaatgtga
     1921 ttcctttgcc tcaactacaa gttaaatatc agaacgtatg acaatggaca aaagagatgc
     1981 tgaaaatacg ccgctaaagg atttgattga tcgctttttg aaatcgtatc gtttggataa
     2041 aaagatgaaa gaaatggagg tcatcaacgg ctggaaagaa atgatgggcg ttgctgttgc
     2101 caaccgaaca gagaggttgt atatcaaaaa caaggtgttg catattaaac tcaattctgc
     2161 tgtaatgcga gaagaattag ccaatggcaa acaaatcatt ttattgcgcg taaatgaatt
     2221 tgcaggtttt gaaataataa atgatatttg gtttgagtaa ctacctaaaa agaactttcc
     2281 taaaaatgat ggctacaata acgctataaa aagttgaaag catcaacatc gataaaatac
     2341 cataagtcat aataaaacca ccggaaaaca acatttgagg agtttttcta aattcttgta
     2401 aaatttcctc tctcgacatg atttccaaat ccttgttatt ggctttgatc ttctttagtt
     2461 caacagggtt gtcaattaca gattggaaat aagcttcgcg ctcagccacc atttttcggt
     2521 tgtaatcggg atcaattttc tcgtaataaa aataaagaaa agcgctgata atcatagaat
     2581 aaacaacccc agccgacatt ccatttttta tatccgtcaa aatattatca tcttcaacgc
     2641 cttttctttt ttgcaaatac aaaccaattg caacagctgc taacaacaat aacatattgg
     2701 tcattacaaa caaggccgta ttgctaggta cgttccaacc aactccaaaa ctcaacattt
     2761 tgaaaccaat ccaaacaagt gcacaaataa tggctgtttt aaatgttact ttcatggttt
     2821 tagctattca tcgtcaccaa aaactcttcg ttggattgcg ttgcatccat gcgttctttc
     2881 ataaattcca ttgcttcaac aggattcata tctgcaatga atttacgcat caaccaaaca
     2941 cgttgcaata catcttttgt aaccaataaa tcttctctac gtgtgcccga agcagtgata
     3001 tcaattgctg gataaattct acggtt
//
LOCUS       DAOQVQ010000286         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_38025_length_3018_cov_2.872764, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000286 DAOQVQ010000000
VERSION     DAOQVQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38025_length_3018_cov_2.872764"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PKN22_08470"
     CDS             <1..1419
                     /locus_tag="PKN22_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNS42778.1"
                     /translation="FFSINDIDFEKETIVRREITKQGKSRAFINDTPVSLAQLKEFTE
                     QLVSIHSQYNTLELKRKDFQFNTLDTLASTDKLHQTYLLQFNDLKKLQKEIAEQKELL
                     FQSAKEKDYNDFQLAELSELNIEKNDFSALEEQLQKVENANEIQQLSQEISQALASDE
                     GVIAQLQQLKSVVQKLARLDKNAGAIEERIASAVIELKDINEEMESFDSAESINEEKR
                     LQLMQLVDQFNHVLSKHRCANQEELLAFYKKLQQSTASLSELEEKIVQLEQKEQQLLK
                     QLNESTSVLHESRKKAIPQLEAQLAKLLVELKLPDTQLVFDLKETDHLHVFGKSTLVY
                     LFSANKGFSPVEIEKAASGGELSRVMLALQQLISEKVELPTIFFDEIDTGVSGDVAQK
                     IGNLLHQMGKNMQLFAISHLPQVAAKADFHLKVEKSNALDATQTTISVLNETERIEEI
                     ARLMSGEIINEAAIANAKALMN"
     gene            1500..2939
                     /locus_tag="PKN22_08475"
     CDS             1500..2939
                     /locus_tag="PKN22_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNS42779.1"
                     /translation="MTKANGYLFIFYFAITTFLLASCGEKTEDDASKQEVLESVKDYQ
                     EIMQRGYLKVLAENSSTSYFIYKGEKMGFEYEILREFAKEMGLELEVEIVANLDNLIP
                     MVNNHEGDIIACNYTVTRERTKEINFSTPYLQTPQILVQKKPDGWEKMTEEEIKKKLI
                     TSPTSLAKKHINVWENSSYYKRLLHLQEEIGDTIYINPVNGLTGSEELIEQVSLGIID
                     YTIAEQNIGLVNARFYDNIDVSTEISVKQNIAFGLRKSSDLLKIKLNKWLKNFLTSSK
                     YKYIYRKYFLIGVPGMDPNDTFSSIGKGEISPYDQFFKAAGKKYNVDWQLLAAIAYHE
                     SRFNASIEGFGGAYSMLQFMPNTGPKYGVYPDSPPEVQIYGGMKKLAADFSYYKDIKD
                     NEQKIKLILATYNAGRGHIDDAVRLAEKYGLDPQKWDDNVGKMVINISKQEFYRDEVV
                     KSGAMKGEFTSKYVDKVYKRYKEWKSTFK"
BASE COUNT         1129 a          458 c          548 g          883 t
ORIGIN      
        1 ttttttagta tcaacgacat tgattttgaa aaagaaacca ttgttagaag agaaattacc
       61 aaacaaggaa aatcacgcgc ttttatcaac gatacacctg tttcattagc acaattaaaa
      121 gaatttacag agcaattagt ttccatacat tcgcaataca atacgctcga actaaaacgt
      181 aaagactttc aattcaatac gttagataca ttggcttcaa cagataaatt gcatcaaact
      241 tatctattgc aattcaatga tttaaaaaaa ttacaaaaag aaattgcaga acaaaaagaa
      301 ctgttgtttc aatcggcaaa agaaaaagat tacaacgatt ttcaattggc tgaattaagt
      361 gagttgaaca tagaaaaaaa tgacttttcg gcgttagaag aacaattgca aaaagttgaa
      421 aatgccaatg aaattcagca attatcacaa gaaatttcac aagcattagc aagtgatgaa
      481 ggtgttattg cgcaattgca gcaactaaaa tcggttgttc agaaattagc ccgtttggat
      541 aaaaatgcag gtgcaattga agaacgtatt gcttcggctg ttattgaact gaaagacatc
      601 aacgaagaaa tggaaagttt tgattctgct gagtctatca acgaagaaaa acgtttacaa
      661 ttgatgcaat tggttgatca atttaatcat gtgttatcaa aacaccgatg tgctaatcag
      721 gaagaattgc ttgcttttta caaaaaattg caacaatcaa ctgcctcgtt gtctgagttg
      781 gaagaaaaaa ttgtgcaatt agaacaaaaa gaacaacaac tgttaaaaca actaaacgaa
      841 tctacatctg ttttgcatga aagccgtaaa aaagctattc cacaattgga agcacaattg
      901 gctaagttgc ttgtagaatt aaaattaccc gatacacagc tagtttttga tttaaaagaa
      961 accgatcacc ttcatgtctt tgggaaatca acattggttt atttattttc agccaacaaa
     1021 ggtttttcgc cagttgaaat agaaaaagca gcaagtggtg gagaattatc tagagtcatg
     1081 ttggcattgc aacaattaat ttcagaaaaa gtagaattgc ctactatttt ctttgacgag
     1141 atagacacgg gtgtttctgg ggatgtggct caaaaaattg ggaatttatt gcatcaaatg
     1201 ggaaaaaaca tgcagttgtt tgccatctct catttgcctc aagtggcagc aaaagcagat
     1261 tttcatttaa aagtagaaaa atcaaatgct ttggatgcaa cacaaactac tatttctgtt
     1321 ttaaatgaaa ctgaacgtat cgaagagatt gcacgtttaa tgagtggtga aataatcaat
     1381 gaggcagcaa ttgccaatgc aaaagcgttg atgaattaac aactgaaaaa gcataattct
     1441 ccctaaaaat ttaaaattct tattattttt tatatcttta atcactctaa gtatagtgaa
     1501 tgacaaaagc taacggttac ttatttatct tttattttgc aataactacc tttttgttag
     1561 ctagttgtgg tgaaaaaacc gaagatgatg ccagcaaaca agaagtactt gaatcggtta
     1621 aagattatca agaaattatg caacgcggct acctgaaagt tcttgcagaa aacagttcaa
     1681 cctcctattt tatttacaaa ggtgaaaaaa tggggtttga atacgagatt ttgcgtgaat
     1741 ttgctaaaga aatggggttg gaattggaag ttgaaattgt tgcaaatctt gacaatttaa
     1801 taccaatggt aaacaaccat gaaggcgaca tcattgcatg taattatacc gtaaccagag
     1861 aacgaactaa ggaaattaat ttttcaactc catatttgca aacacctcaa atattggttc
     1921 aaaaaaaacc tgatggctgg gaaaaaatga ccgaagaaga aattaagaaa aagttaatta
     1981 cttcgcccac tagtttagcc aaaaaacaca tcaatgtttg ggaaaattca agctattaca
     2041 aacgtttatt acacctgcaa gaagaaattg gtgatactat ttatatcaat ccggttaacg
     2101 gtttaacagg tagcgaagag ttgattgaac aagtatcttt aggaattatt gattacacca
     2161 ttgctgaaca aaatataggt ttagtcaatg cgcgttttta tgacaacatt gatgtttcta
     2221 ccgagatttc cgtaaaacaa aacattgcct ttggtttaag aaaatctagc gatttgttaa
     2281 aaataaaatt gaacaaatgg ctaaaaaact tcctaacttc gtctaaatac aaatacattt
     2341 atcgtaaata tttcttaatt ggtgttccgg gcatggatcc aaatgacacg tttagttcta
     2401 taggaaaagg tgaaatttct ccttacgacc aattttttaa agcagcagga aaaaaataca
     2461 atgtagattg gcagttattg gctgctattg cttatcatga aagtagattc aacgcctcaa
     2521 ttgaagggtt tggtggtgct tatagtatgt tgcaatttat gccaaatact ggtcctaaat
     2581 atggcgttta tcccgattct cctccagaag tacaaattta tggtggaatg aaaaaattgg
     2641 ctgctgattt tagttattac aaagatatca aggacaatga gcaaaaaatt aaactcatat
     2701 tagcaaccta taatgctgga agaggtcaca ttgatgatgc cgttcgattg gctgaaaaat
     2761 atggcttaga cccacaaaaa tgggatgaca atgttggaaa aatggtaatt aacatttcaa
     2821 aacaagaatt ttaccgcgat gaagtagtaa aaagcggtgc catgaaaggt gaatttacaa
     2881 gtaaatacgt agataaagta tacaaacgct acaaagagtg gaaatcaact tttaagtaaa
     2941 aattgataca atgaaagaat taattgtcat tggtggacct acagccagtg gaaaaacagc
     3001 gttgagtatt gctttggc
//
LOCUS       DAOQVQ010000287         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_38433_length_2997_cov_3.538069, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000287 DAOQVQ010000000
VERSION     DAOQVQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38433_length_2997_cov_3.538069"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKN22_08480"
     CDS             complement(<1..1182)
                     /locus_tag="PKN22_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42780.1"
                     /translation="MKKILITALYTLACLFSYVNAQSNDCFTANPFCSSNTYNFPNAT
                     SGTAPAGPNYGCLGSVPRPIWYYMEIGTSGTMQIGLSQTDNSGWGIDVDFAMWGPFSD
                     LASGCTQVMSGGVAPLQCSFSASDTETLGLGMQGGTGTGQSTPPAAVAGQVYIVLLTN
                     YDGDAGTIDFNQTGGTGSADCAIVNPCVINNLTGIVSACNTATNLYSVSGNVTISNPP
                     TTGNLIATACDGTQTVIATAPFGAASYPYTLNGLNSNGAACNVTVTFTDGACSQVLNY
                     TAPTCPAACNFTDINATIGSCEPGSVFDLTGSVQFVNAPATGQLIIQDCNGNSQTFNA
                     PFTSPTNFTINNITADGAACSVTATFTADAACTISMNYTNTTSCNCGANIGTFTVTND
                     GT"
     gene            complement(1195..1635)
                     /locus_tag="PKN22_08485"
     CDS             complement(1195..1635)
                     /locus_tag="PKN22_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42781.1"
                     /translation="MKKYHLFLLLLIASTTLYGQANDRPQQIKLTDKKDIKATQQPQT
                     KTTSNSTTTENSSNSNSQSYPISYTVDAAGNQETMDKAFYERQLEGVNDLIEAIDYKV
                     EYVKSNPEENAKALNSGWYEQMKRSRINAENRKLEIQEKIQSFK"
     gene            complement(1735..2484)
                     /locus_tag="PKN22_08490"
     CDS             complement(1735..2484)
                     /locus_tag="PKN22_08490"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNS42782.1"
                     /translation="MSQPTKIYFASDFHLGAPNYEKSLEREKKIVAWLDTIKKDATEI
                     FLVGDIFDFWFEYKKAIPRGFARLQGKIAEITDSGIPVHVFTGNHDMWIFDYLPKELG
                     VQLHREPIVREWNGKTFYIGHGDGLGPGDRGYKFIKKVFASPVCQWLFARLHPNFGIG
                     LADFFSRKSRAATGNSDEKFLGEENEWLYIYSKEYLQKQHIDYFVFGHRHLPLEMPVE
                     NNSIYYNLGEWINYQTYLVFDGNNCSLSKWV"
     gene            complement(2486..>2997)
                     /locus_tag="PKN22_08495"
     CDS             complement(2486..>2997)
                     /locus_tag="PKN22_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS42783.1"
                     /translation="SFGQSKKIQELATYYEQTKAKNEAANMFAPGKLAPDFEELKLDQ
                     KTKMKLSDLRGKVVLLDFWASWCGPCRRENPNVVNVYNKYKDKGFTVMSVSLDGDLES
                     WKRAIEQDGLIWPNHVSDLKKWSSAVGRIYQVNSIPFTVLIDQEGKIVTTNLRGPALE
                     EVVSKLVSQ"
BASE COUNT          894 a          586 c          519 g          998 t
ORIGIN      
        1 tgttccatca tttgtgacgg taaatgttcc aatattagcc ccacagttgc aggaggttgt
       61 attggtatag ttcattgaaa tcgtacaagc agcatctgca gtaaaggtgg ctgttacgct
      121 acatgctgct ccgtctgctg tgatattatt aattgtaaag tttgtaggac ttgtaaatgg
      181 cgcattaaat gtttgcgaat ttccgttaca atcttgaata atcaattgac ctgttgcagg
      241 agcgtttaca aattgcaccg aacctgttaa atcaaaaaca ctacctggtt cacaagatcc
      301 aatagtggca ttaatatctg taaagttaca agctgcggga caagtaggtg ctgtgtaatt
      361 taaaacttga gaacaagctc catccgtaaa agttacagtt acattacacg ctgcaccgtt
      421 agaatttagt ccatttaacg tataaggata agatgctgct ccaaatggtg ctgttgcaat
      481 aactgtttgc gtaccatcac atgctgttgc aattaaattc cctgttgttg gcgggttgga
      541 aatggttacg ttacctgaaa cagaatataa attagttgca gtattacaag ctgaaacaat
      601 tcctgttaag ttattgatga cacaagggtt gacaatagca caatctgcag aaccagtacc
      661 cccagtttgg ttaaaatcta tggtacctgc atctccatca taatttgtta acaatactat
      721 atatacttga ccagcaacag cagcaggtgg cgttgattgc ccagtgccag ttcctccttg
      781 cattcctagt cctaaagtct ctgtatcaga tgctgaaaaa ctacactgaa gtggtgcaac
      841 acccccagac ataacttgtg tacatccaga tgctaaatca gaaaagggac cccacatagc
      901 aaaatccacg tcaattcccc agcctgaatt gtcagtttgt gataatccta tttgcattgt
      961 tccagatgtc ccaatctcca tataatacca tattggtcta ggaacagaac caagacaacc
     1021 ataattaggt cctgctggag ctgtaccaga agttgcattc gggaaattat acgtattaga
     1081 agaacaaaac ggattggccg taaaacaatc atttgattgc gcatttacat acgaaaacaa
     1141 acaagctaaa gtatataaag cagtaattag tatctttttc ataagtttca atatttattt
     1201 aaatgattga attttttctt gaatttctaa ttttcgattt tcagcattta ttcgagagcg
     1261 tttcatttgc tcataccatc ctgaatttaa cgcttttgca ttttcttctg ggttgctttt
     1321 aacgtattcc actttgtaat ctatggcttc aatcaaatcg ttaacacctt ctaattgtcg
     1381 ctcgtaaaat gctttatcca tcgtctcctg atttccagca gcatcaactg tataagatat
     1441 tggataagat tgcgagttgg aattggatga gttttctgtt gtagttgaat tggatgtagt
     1501 ttttgtttga ggttgttgtg tagcttttat atctttttta tccgttagtt tgatttgttg
     1561 cggcctgtca tttgcttgac catacagcgt agtacttgcg attagtagca agagaaataa
     1621 gtgatacttt ttcatggttt tcaaaagtta attcataatc tgttaacaat attacgaatt
     1681 aatttaaaac cagttgcctg tcaattaaaa aaaaattaac atttttttaa acgactaaac
     1741 ccatttagac aaactgcaat tgttgccatc aaacaccaaa taggtttggt aattaatcca
     1801 ttcacctaaa ttgtagtaaa ttgagttatt ttctactggc atttcaagtg gcaagtggcg
     1861 gtgaccaaaa acaaagtaat ctatgtgttg tttttgtaaa tattctttgg aataaatata
     1921 caaccattcg ttttcttcgc ctaaaaattt ttcgtcggaa ttgcctgttg ctgccctact
     1981 ctttctggag aagaaatcgg ctaatccgat accaaagttg gggtgcaggc gtgcaaataa
     2041 ccactgacaa actggtgagg caaatacttt tttgatgaat ttataccctc tatcaccagg
     2101 gcctaaacca tcaccatggc caatgtaaaa tgtttttcca ttccattctc taacaatagg
     2161 ttcacggtgt aattgaacgc caagttcttt tggtaaataa tcaaaaatcc acatatcgtg
     2221 gttacctgta aatacgtgaa cgggaatacc gctatcggtg atttctgcaa tttttccttg
     2281 caagcgtgca aaacctcgag gaattgcttt tttgtattca aaccaaaaat caaaaatatc
     2341 gccaactaaa aaaatttctg tagcgtcttt tttaattgta tccaaccacg ccacaatttt
     2401 cttttcacgc tctaaacttt tttcataatt gggagctccc aaatgaaaat cggatgcaaa
     2461 ataaattttg gttggctgcg acattttatt ggctcactaa ttttgatacg acttcttcta
     2521 atgctggtcc acgcaaattg gttgtaacaa tttttccttc ttggtcaatc aacacggtaa
     2581 aaggaataga gtttacttga taaattctac caacagcact gctccatttt ttcaaatcgc
     2641 tcacatgatt aggccaaatt aaaccgtctt gttcaatagc acgtttccaa ctttctaaat
     2701 caccgtctaa agaaacactc attaccgtaa atcctttgtc tttgtattta ttgtaaacgt
     2761 ttacaacatt tggattttct ctgcggcatg gtccacacca cgatgcccaa aaatctagca
     2821 acaccacttt tcctcgtaag tcggaaagtt tcattttcgt tttttggtcc aatttcaatt
     2881 cttcaaaatc gggtgctaat ttacctggag caaacatatt agccgcttca tttttagctt
     2941 tcgtttgctc gtaatatgta gcaagttctt gaattttctt gctttgacca aagctag
//
LOCUS       DAOQVQ010000288         2982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_38710_length_2982_cov_4.935770, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000288 DAOQVQ010000000
VERSION     DAOQVQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38710_length_2982_cov_4.935770"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="PKN22_08500"
     CDS             complement(<1..1943)
                     /locus_tag="PKN22_08500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNS42784.1"
                     /translation="MNLDFSRILEVFSFQFNPDTAFLFTKVEFWIFFVALMIAYMLLE
                     ANTYVRTAVISTVVLLTSWLFQNGDVIVFGALCILNYAFLYALFKAQKKYNWLFLILS
                     INALGVYYFWFKLTYFQIYFTLRPIDVWLLFLLLIFVLSFFKQKLLVRSIFFTFVSLY
                     FYYKTSDKFVYLLLFSIVFNYSMGRWVFATISKAGKKWLVALAVIVNVGILAYFKYSY
                     FFTDSFNTIFGTQHVPTNVFAEWANWLLGKPTFVTDILLPVGVSFITFQTISYVVDVS
                     RGEVEPVKNIFNYAFFATFFPPLVSGPILRAKDFVPQIYKPFQLTKADYSLAVIMIVT
                     GLLKKVVMADYIAVHFIDKVADAPTAYPGFVSIMAMWGYSLQIYGDFSGYTDMATGIA
                     LLMGFRLPENFNSPYKSVSVADFWRRWHKSLGAWLKNYLYIPLGGNRSGGLGTFIATF
                     FILIFLVFITRWYELFYVYFGLMFIYIIAVRLIPAEKTKLYRDLNLLITLVVGGLWHG
                     ASENFVIWGAMNGVALVIYNYWKLISPYENKNGWLVHFWKVFITFNFITFTRIWFRLD
                     EAGMPNQMLDQIWNHFDFAWERFVLVLQTYQTSLWIILIGFCIHWLPTRWKDAYKNLF
                     IKMPVPLQLIAVSCIIFLMNKFL"
     gene            complement(2048..2572)
                     /locus_tag="PKN22_08505"
     CDS             complement(2048..2572)
                     /locus_tag="PKN22_08505"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNS42785.1"
                     /translation="MLMPFNLQKWIDENRDLLKPPVGNKNLYVDAGDFIVMIVGGPNA
                     RKDYHFNESEELFYQLEGDILVKIQHEGQAKDIHIKEGELFLLPANTPHSPIRGANTV
                     GLVIERVRKQTDLTDGLMWFCEKCNNKLHEYRFPLEDIERDFLPRFKEFYTSKDLRTC
                     KKCGHEMEVDPRFV"
BASE COUNT         1033 a          580 c          567 g          802 t
ORIGIN      
        1 tataaaaact tattcatcaa aaagatgatg caagaaacgg caatcaattg caacggaact
       61 ggcattttga tgaataagtt tttataggca tctttccatc gagtaggtag ccagtgtatg
      121 caaaatccga tgagaataat ccaaagtgat gtttgatagg tttgcaacac caacacaaaa
      181 cgttcccacg caaaatcaaa gtgattccag atttgatcga gcatttgatt gggcattcct
      241 gcctcgtcta aacggaacca aatacgggtg aaggtaatga aattaaaggt gataaaaact
      301 ttccaaaaat gcaccaacca tccgtttttg ttttcatacg gtgaaatgag tttccaatag
      361 ttgtaaatta ccaaggcaac accgttcatg gcgccccaaa ttacaaagtt ttcgcttgct
      421 ccatgccata aaccaccaac aacaagtgta atgagtaggt ttaaatcgcg gtataatttt
      481 gttttttcgg ccggaattaa acgaacggca atgatataga taaacatcag tccgaaataa
      541 acataaaaca gctcgtacca gcgggtgatg aacaccaaga aaatgagaat gaaaaacgtg
      601 gcaataaaag ttcctaaacc accacttcgg ttacctccca acggaatgta gaggtagttt
      661 tttagccaag cacccaacga tttgtgccaa cgacgccaga aatcggcaac gctaacagat
      721 ttgtagggtg aattgaagtt ttctggcaaa cgaaatccca tcaacaaagc aataccagta
      781 gccatatcgg tatatcccga aaagtcgcca taaatttgca aggaataacc ccacatggcc
      841 ataatgctca caaaacctgg gtaggctgtt ggtgcatcag ccaccttgtc aatgaaatga
      901 acggcaatat aatcggccat tactactttt ttgagcaaac cggtaacgat catgataacc
      961 gccaaactgt agtcggcttt ggtaagttga aacggtttgt aaatctgcgg aacaaaatct
     1021 tttgcgcgca aaattgggcc cgaaaccaaa ggtggaaaga acgttgcgaa aaaagcgtaa
     1081 ttaaaaatat ttttaactgg ttccacctca ccacgcgata catccaccac atagctgatg
     1141 gtttgaaacg tgatgaacga aacgcccact ggcaataaaa tatcggttac aaaagtaggt
     1201 ttgcccaaca accaattggc ccattctgca aatacgttgg ttggaacgtg ttgggttcca
     1261 aaaatagtgt tgaatgaatc ggtgaaaaaa taagagtatt tgaaataggc aagaattccc
     1321 acgttaacaa tcactgcaag ggctaccaac cattttttac ctgctttgga aatggttgca
     1381 aaaacccaac gtcccatgga atagttgaac acgatggaaa acaacaacag gtaaacaaat
     1441 ttatccgatg ttttgtagta aaaataaagg ctgacgaagg taaaaaagat ggagcgaacg
     1501 agtaattttt gcttaaaaaa cgacagcaca aagatgagca acaaaaacag caaccacacg
     1561 tcaatggggc gcagtgtgaa atagatttgg aaatacgtga gtttgaacca aaaatagtaa
     1621 acacccaagg cgttgatgct gagaattaaa aacagccaat tgtatttttt ttgtgctttg
     1681 aacaaggcat acaaaaaggc ataattgagg atgcaaagtg ctccaaaaac tatgacatca
     1741 ccgttttgaa acaaccaact ggttagcaac acaaccgttg aaatgacagc tgtgcgcacg
     1801 taggtatttg cttcgagcaa catgtaggca atcatcaatg ccacaaagaa aatccagaat
     1861 tcaacttttg taaataaaaa agccgtatca ggattaaact gaaaggaaaa aacctctaaa
     1921 attctgctaa aatccaaatt catattgtgc gtgctaaaca gcgttgcgaa aatagatatt
     1981 taggagggat aaatagcaaa ggtcggatga aaaatctttc acatctcctt tttaataatg
     2041 cctagcacta cacaaaacgt ggatccactt ccatttcgtg gccacatttc ttacaagttc
     2101 taaggtcttt tgatgtataa aactctttga atcgaggcaa gaaatcgcgt tcgatgtctt
     2161 ctagcgggaa gcggtattcg tgcaatttat tgttgcattt ttcgcaaaac cacatcaagc
     2221 catcggttaa atcggtttgt tttctaacgc gttcaatcac aagtccaacc gtatttgctc
     2281 ctctaattgg gctgtgtggc gtattggctg gtagtaaaaa cagttcccct tctttgatgt
     2341 gaatatcttt tgcttgtccc tcgtgttgta ttttcactaa aatatcgcct tcgagctggt
     2401 aaaacaattc ttcactttcg ttgaaatggt aatcttttcg ggcatttgga ccaccaacta
     2461 tcattacgat aaaatcgccg gcatccacgt ataaattttt gtttcccacg ggtggtttta
     2521 gcaaatcgcg gttctcatca atccattttt gtaagttaaa gggcattagc atagcattct
     2581 ttttatttta aattaatggt tgttcaccaa tgaaattttg ttgattcaaa tatacgtttt
     2641 ttattttaca aagtaaggtt tgttttgagg ggggatagtt ttttcgatct gagttatcca
     2701 taagttagct acaacccaaa agaacaggcg aacacacttt cctgagccta acgaaggaag
     2761 ccaacgctca aaaaaaacaa aagagccatt ttttttacca acacacagac atcgtttcga
     2821 cataaatact tatcttaacc aactgaaata gaaaatttat tttattagat gaaatctgct
     2881 tgcggattcg tgaacaccaa taaaaaaaca aaaagatctg tgaacaattt aaaagaattg
     2941 aaaccaagaa aggcactgaa caaagccttt ttaaaagtaa aa
//
LOCUS       DAOQVQ010000289         2982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_38719_length_2982_cov_3.245986, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000289 DAOQVQ010000000
VERSION     DAOQVQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38719_length_2982_cov_3.245986"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKN22_08510"
     CDS             complement(<1..492)
                     /locus_tag="PKN22_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS42786.1"
                     /translation="MLTVNNLSLQFGKRVLFDDVNVKFVNGNCYGVIGANGAGKSTFL
                     KILTGEQDPTTGRVELEPGKRLAVLNQNHFAFDSEQVLQTVIMGHRRLYEIMTEKDAL
                     YAKPDFSDEDGMRTAELEAEFADLEGWNAETDAATLLSNLGIDESKHYLLMEELSNDL
                     KVRV"
     gene            622..1209
                     /locus_tag="PKN22_08515"
     CDS             622..1209
                     /locus_tag="PKN22_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNS42787.1"
                     /translation="MKRSTIIILVVIGILMFWVIGVQRKAVTLSESIENSWGNVETAY
                     QRRNDLIGNLVKTVQGSADFERKTLEAVVNARAKATQTKIDPSNMTEEDFKRFEEAQA
                     NVKSSLDRLLVVVENYPDLKSSSNFLKLQDELASTENQILTARTRFNDEVKPYNTYIK
                     KFPTNLIAGMFGFTERQYFKSVEGADKAPDVEFNF"
     gene            1212..1655
                     /locus_tag="PKN22_08520"
     CDS             1212..1655
                     /locus_tag="PKN22_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNS42788.1"
                     /translation="MDAPTPSDFLSKEEVQEVVLAIKEAECNTSGEIRVHIDQEEVES
                     PFEHAKIVFDELEMYTTQDRNGILFYFNISTHSFVIIGDEGIHQKVGDEFWDIIRNAV
                     INEFTKGNYKVGLINGISEVGKQLKSHFPYQANDKNELPNEISGI"
     gene            1636..2415
                     /locus_tag="PKN22_08525"
     CDS             1636..2415
                     /locus_tag="PKN22_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNS42789.1"
                     /translation="MKFQASKLYRIFLLLLAIGWSLQLFAQFDIPPKPSKQTALYDYA
                     KLLTPEQATSLEQKLVHYFDTTGNQIVIAIVPTIKGEDIGLLTPKWGHEWGVGGKEND
                     NGVFILLAVEEHKIYIAPGYGLEHLLTAGINGEIIRNDIIPHFKQGDYYTGLMVGTDQ
                     LMKLFSGAYQPTAVEEPFPWGALLIFLGIVVLVFYLNYKNKKEGGGGFGTGGGAYTIG
                     RYGGGYYGGGSSRSSGGFGGGGFSGGFGGGGFSGGGAGGSW"
     gene            complement(2473..>2982)
                     /locus_tag="PKN22_08530"
     CDS             complement(2473..>2982)
                     /locus_tag="PKN22_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNS42790.1"
                     /translation="GTSIGVYKRFEQIGFKHIAGVMVKEDVLLGGEESGGIAVKGHIP
                     ERDGIWMGMIIWEFMAKSGKTLDELINEVYEIVGEFKFERNDLHISEELKQAIIKNCN
                     ENIYKEFGKYRINEVKTIDGFKFFFDDERWVMIRPSGTEPVLRIYAEAPTLDEAREIL
                     KATINQICN"
BASE COUNT          950 a          536 c          586 g          910 t
ORIGIN      
        1 aacacgtact tttaaatcgt tggacaattc ttccatcaat aaatagtgtt ttgactcatc
       61 aattccaagg ttgctcaaca aggttgcggc atctgtttct gcgttccatc cttccaaatc
      121 ggcaaattcg gcttccaatt cagcagtacg cattccgtct tcgtcagaaa aatcaggttt
      181 agcatacaat gcatcttttt cggtcatgat ttcatacaaa cgtctatgtc ccataatcac
      241 tgtttgcaac acttgctcgc tatcaaaagc aaagtgattt tggttcaata ctgccaaacg
      301 ctttcctggt tccaattcca ctctacccgt tgttggatct tgttcaccag ttaatatttt
      361 taagaatgtt gattttccgg ctccattagc gccaatgaca ccgtaacagt ttccgttaac
      421 aaacttaacg ttcacatcat caaataaaac tcgtttccca aattgaaggg acaagttgtt
      481 aactgtaagc atatttttga aaaatttagt gcaaagatag tgattttata tgaattggag
      541 cggtgaaaaa attatccgac taaagttgtt gaaatcaaat aatttctact aaatttatct
      601 gaaaaaataa aaagtaaaac tatgaaaaga agtacgatta tcattttagt tgtaattggg
      661 attttaatgt tttgggtaat tggcgttcaa cgcaaagccg taacattaag cgaaagcatt
      721 gaaaacagtt ggggaaatgt agaaacagct tatcaaagaa gaaatgattt gattggtaat
      781 ctagtgaaaa cagttcaagg ttcggctgat tttgaacgca aaacattgga agctgttgtt
      841 aatgcacgtg caaaggctac gcaaactaaa attgacccgt caaacatgac tgaagaagat
      901 tttaaacgtt ttgaagaagc acaagccaac gtaaaatcat cgttggatcg tttattggta
      961 gttgttgaaa attatcctga cttaaaaagc agtagtaatt tcttaaaact tcaagacgaa
     1021 ttggcaagta ctgaaaatca aattttaact gctcgcaccc gtttcaacga cgaagtgaag
     1081 ccttataata catacatcaa aaaattccca accaatctta ttgctggtat gtttggattt
     1141 acagaacgtc aatatttcaa aagtgtggaa ggtgctgaca aagctccaga tgtggaattt
     1201 aatttttaac aatggacgca cccactccat ccgactttct ttctaaagaa gaagttcaag
     1261 aagttgtttt agccattaaa gaagcagagt gtaatacgtc tggtgaaatt cgtgtgcaca
     1321 ttgatcaaga agaggtggaa tcaccctttg aacatgcaaa aatagttttt gatgaattgg
     1381 agatgtatac aacacaagat agaaatggaa ttttatttta tttcaatatt tccacccatt
     1441 cttttgtaat aataggcgat gaaggaattc atcaaaaagt aggtgatgaa ttttgggata
     1501 ttattcgaaa tgccgtcatc aatgaattta caaaaggaaa ttataaagtg ggattaatca
     1561 acggaattag tgaagtagga aaacaattaa aaagtcattt tccttaccaa gcaaacgata
     1621 aaaacgaact tccaaatgaa atttcaggca tctaaactat atcgaatatt tttgttgttg
     1681 cttgcaattg gttggagttt gcaactattt gcccaatttg acattccgcc caaaccgtca
     1741 aaacaaacgg ctttgtatga ttatgctaaa ttgctaacac ctgagcaagc aacaagttta
     1801 gagcaaaagt tggtacacta ttttgacacc accggaaacc aaatagttat agccattgtg
     1861 ccaaccatca agggtgaaga cattggtttg cttacgccaa agtggggaca tgaatggggt
     1921 gttggtggaa aagaaaacga caatggtgtg tttattcttt tagctgttga agagcacaaa
     1981 atatacattg caccaggcta tggtttagaa caccttttaa cggctggaat aaacggagaa
     2041 attattcgca acgacatcat accgcacttt aaacaaggag attactacac tggtttaatg
     2101 gtgggaacag atcaactaat gaaattgttt agcggagcct accaacccac agccgtagaa
     2161 gagccttttc cttggggagc tttgttgatt ttcttaggaa ttgtggtgct tgtgttttat
     2221 ttgaattata aaaataaaaa agaaggcggc ggtggttttg gaactggcgg aggtgcctat
     2281 actattggtc gctacggtgg cggatattat ggcggaggca gttctagaag cagtggcggt
     2341 ttcggaggag gtggattcag tggtggcttt ggcggtggcg gctttagcgg tggtggtgct
     2401 ggaggaagtt ggtagtgaca agacataaaa aaaggagcaa tcattgctcc ttttttagtt
     2461 attgaacatc tatcaattac aaatttgatt gatggttgct tttaatattt cgcgtgcttc
     2521 atccaaggtt ggcgcttcgg cataaatgcg caaaacaggc tctgttcctg aaggacgaat
     2581 catcacccaa cgttcatcgt caaagaaaaa tttgaatcca tcaatggttt taacttcgtt
     2641 gatgcggtat tttccaaatt ctttataaat gttttcgttg caatttttga tgatggcttg
     2701 tttcaattct tcggaaatgt gcaaatcgtt gcgttcaaat ttgaattcac ctacaatttc
     2761 atacacttca ttgatgagct cgtcaagagt tttgcccgat tttgccataa attcccagat
     2821 aatcatcccc atccaaatac cgtctcgctc tggaatatgt ccttttacgg caataccacc
     2881 cgactcttct cctcctaata acacatcttc tttaaccata actccagcaa tgtgtttaaa
     2941 cccaatttgc tcaaaacgtt tatacacacc gattgaagtg cc
//
LOCUS       DAOQVQ010000290         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_38898_length_2972_cov_3.320192, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000290 DAOQVQ010000000
VERSION     DAOQVQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38898_length_2972_cov_3.320192"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1341)
                     /locus_tag="PKN22_08535"
                     /pseudo
     CDS             complement(1..1341)
                     /locus_tag="PKN22_08535"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
     gene            complement(1473..2204)
                     /locus_tag="PKN22_08540"
     CDS             complement(1473..2204)
                     /locus_tag="PKN22_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS42791.1"
                     /translation="MRALYFKEIRSFLSSIIGFIFVFIFLVATGIFHWIVPGGTNFLE
                     GAEADLIPFFNLAPILFFILIPALTMRSIAEERRTGTIELLFTRPISDLKIILAKYFA
                     GVTLLVIALIPTVIYYISMYNLALEPEKFDHGATITSYFGLILLGAVFVAIGIFSSSL
                     TSSQIVAFIISMFLCWLFFDGLNRLGAFNLMGQFDVVLQYFGMVKHYDSIRKGVIDTS
                     DVIYFLSLIVFFIFSALTVIKTLKK"
     gene            2455..>2972
                     /locus_tag="PKN22_08545"
     CDS             2455..>2972
                     /locus_tag="PKN22_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNS42792.1"
                     /translation="MKKILIKNIKGLVQASEDLPTTISGKNMNNLPIIENAFLAIENG
                     EIVAYGPMEDWQGITDWRDLEVIDATDKFVMPAFIDAHTHTVFAKSREEEFVDRIKGL
                     SYEEIALKGGGILNSARRLGELSEDELYESAKIRVERMISYGTGALEIKSGYGLTVEN
                     ELKMLRVIKRLK"
BASE COUNT          951 a          517 c          543 g          961 t
ORIGIN      
        1 tgaatcgtat ctattggcaa taaaacgacc gtttccaacc aacatcactt tactttcttt
       61 ttcactttga ggtgtaaatt gtgcatctgg atttttggtg tactcatcaa caattcttgt
      121 tttaaaatac gattgaaaca ttccttctga taaacctgcc aagcattttt tattgctttc
      181 taatgttgga ttatcagtta aaattgggtt gttgccataa ttcaacggta aacctaaatt
      241 taccaaaggt gccaatcctg ttgtagtggc attggtggat gaagttaaaa ttggtgttaa
      301 aacggtgttg acagaattca caaattgcac ttcactagca tattttaacg aaaccgcctc
      361 tagatttcta gaaattggat gcgatgttgg tgttgccatt acgtgaaaaa accaaggtac
      421 tactggtcgg ttggcaaatg gaactccttt aggcgcacat ctggcatcca atacatagtt
      481 ttcgtttaat tttaatccgt agtcaaacag caagtcttcc aacccagttt tgatgcgcgt
      541 tgtatgcgtc attcccttta aattcaatga atcttcgttg atggaaagtg catccatgaa
      601 acacatcaaa cgtccgcctt tcatcaaaaa ttggtcgata acaaacaaat ctttcacatc
      661 aaacttttga cgaggattgg ctataatcaa tccttggaaa ttatccaacg catttaattt
      721 tccatcaatt gttacatctt ccacgctgta atatgacgta atcaatgcgc gtgcacgttg
      781 cgtttatgca aatgttaatt caccatgtcc ctgtaaaaat ccaatttttt gtttttcaga
      841 ttgaatggct ctacggatgg agctggttaa aatgtactcc aagttattga ttgcactgtt
      901 gatggattgc gaaatacctt ccaattggtt gggttttcca gccggtgttc caggtaaaaa
      961 ttgaatcaca tttacatcgg ttccgccata attgatgatt gctccaggcc aaagtaaaat
     1021 ctgactttgc gctccgtctt tttgataaat caagtttaaa ggtaaaattc cttgcccttg
     1081 gttgtataat tggtctttta attctttttg ctcttcttcg gtgccttggt tagggtcgat
     1141 gaacttatat tcaatacgat ttccagcata ttgtctaaat tcattgagtt tatcctgaat
     1201 ggcatttcta aagtttttaa tttctgccgg catcgttcct tccaaataaa tgcggatggt
     1261 aagcctgttt ttaatggcgt tttcgtcttc caacattttt attgttccac ttgaaagtga
     1321 ataacgttgg tcggctgtca tgtcaattcg tttgtacaaa taagcgctaa tcacgttaat
     1381 ggcaataatt cccaccatta caattagcaa caatgtccaa ttaaaaattc ctttgtatga
     1441 ttttttttca ctcatattga aagtaaaaaa gattattttt ttaatgtttt aattactgtt
     1501 aatgcagaga agatgaagaa cacaatgagc gataagaaat aaattacatc gctggtgtct
     1561 atcactcctt tacggatgga atcgtaatgt tttaccattc caaaatattg caaaacaacg
     1621 tcaaattgcc ccattaagtt gaatgctccc aatcggttca atccgtcaaa aaacaaccaa
     1681 caaagaaaca ttgaaatgat gaatgccaca atttggctac tggtcaatga cgatgaaaaa
     1741 attccgatgg ccacaaaaac agctccaagc aaaatcaatc caaaatagga agtgatggtt
     1801 gctccgtgat caaatttttc gggttccaat gctaaattat acattgaaat atagtaaata
     1861 accgttggaa ttaaagcaat aaccaacaag gtaacgccag caaaatattt ggctaagatt
     1921 atttttaaat cgctaattgg tcgtgtaaac aacaattcaa tagttcccgt tcttctttct
     1981 tcggcaatgc tacgcatggt aagtgccgga atgagtatga aaaacaaaat tggtgcaaga
     2041 ttgaaaaaag gaattaaatc ggcttctgct ccctctaaaa aattagttcc tccaggcact
     2101 atccaatgaa aaatacctgt tgcaactaaa aaaataaaca caaaaataaa accgataatt
     2161 gaacttaaaa aactgcgtat ttccttaaaa tagagtgctc tcattgattt tcaaatatta
     2221 tcaaacttca aaaataagat ttctcattga tttgattgct atctaatttt agaaactatc
     2281 tacaattgat tgttggttga gtgaaaagta gctttttttg tgtgattagc aatggattaa
     2341 caaggatgtg agtggaataa cataaatttt gttaaactgt tcaaagtttg atttgtatct
     2401 tcgcaattgg aagaatttat agcacatttc ataaaacagt gaaaatcatt tgccatgaaa
     2461 aaaatattaa tcaaaaatat taaagggttg gtacaagcca gcgaagattt gccaactacc
     2521 atttctggaa aaaatatgaa caatttgcct atcatcgaaa atgctttttt ggcgattgaa
     2581 aacggcgaaa ttgtggctta tggaccgatg gaagattggc aaggaatcac cgattggcgc
     2641 gatttggaag tgattgatgc taccgataag tttgtaatgc ccgcttttat tgatgcacat
     2701 acacacactg tttttgctaa aagtagggaa gaggaatttg ttgatcgcat caagggtttg
     2761 tcctacgaag aaattgccct caaaggtggc gggattttaa attctgctcg cagattgggt
     2821 gaactttctg aagatgaatt gtatgaaagt gccaaaatcc gtgtggaacg aatgattagc
     2881 tacggaacgg gtgctttgga aataaaatcg ggttatggat taacggttga aaatgagcta
     2941 aaaatgttgc gcgttatcaa acgtttgaaa ga
//
LOCUS       DAOQVQ010000291         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_39045_length_2964_cov_3.053283, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000291 DAOQVQ010000000
VERSION     DAOQVQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39045_length_2964_cov_3.053283"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKN22_08550"
     CDS             <1..629
                     /locus_tag="PKN22_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUMOD3 domain-containing DNA-binding protein"
                     /protein_id="HNS42793.1"
                     /translation="KYEQIWIDTFDFNKQLYNFCKVAGNTTGRVVTEETRKKISKKKK
                     GRKMSDAAKKKMSIAQKGRKQTQEAIDKTATAKQKRCVKIDKDTKEIIEIFASFRKAA
                     NSLGLIKAGDLISCARGKLKTSHGFIWKYIDTEDLHLNKDYISKPRTSGAKKINTYDK
                     TTNELFFTFYSATEATKGLKLDPRCIANICKAAVTKDKVAYGYKLEYA"
     gene            complement(688..2142)
                     /locus_tag="PKN22_08555"
     CDS             complement(688..2142)
                     /locus_tag="PKN22_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42794.1"
                     /translation="MSGYAETVLKSFNDQVRNIFVPAGVQNPDEFDVLKKAVGNTNAS
                     QLLYVEGGLFANCMLERPVMNVTITPKRSLANRIPVVRRNTQKSMYAFLTDIAEPSGS
                     LPDYPCDDPQKVGNLTAAFIEVEKGRVSLGSQTLELDKIIQRYHQGITTDLYIVGDVR
                     GVSAGIPAGMEDNLSLLSQAATRRQFQLIARGLQNEVLKQFWTGDPTNVALNTTNGGA
                     KQFWGLEFLVADDYGTKTNVTGTNKTQLNSDIKDFESTCIGGQNANTGLGLYGYMTEL
                     EDTLTGKASLYGYNNVEWVWVMHPTHWSALVKYLPCEMLGDGCGSFLAGGAVANTNGV
                     TVVSNDMGIATIRQQLQSTMRLTINGRSYMVILDDAMPLTITGTNPVSHTGDIYFIPL
                     SVEGVPTLFWDSADYRAVAPALEPIPGGLGGLHGWVDGGIKLSTARHNGFCFDTQTKM
                     ELGLVFIAPHLAGKIENVVACALQQKTIFNDVTP"
     gene            complement(2181..2819)
                     /locus_tag="PKN22_08560"
     CDS             complement(2181..2819)
                     /locus_tag="PKN22_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42795.1"
                     /translation="MGKLNVKKANDWINQGAPEQALDFLGKAVDTLNQTAIEKNIIFK
                     GVENVEVKDEVKSEVVTEEVVTKEETVVAQTETVTEVVDETQTANTQENFMEVIQKGI
                     TDAVLVALKEYNESVVSPLQAKLAELEAKFEPVATDTTTKSYARFQNVFLNASDFMPA
                     AAVSAAMKKEFGVVEKQEVGEVIVSQEEIAKTAVTEKTVTKSYDPNNVLAGF"
BASE COUNT         1005 a          545 c          500 g          914 t
ORIGIN      
        1 ataaatatga acaaatttgg attgatacat ttgatttcaa taaacaattg tataattttt
       61 gtaaagttgc tggtaataca acaggaagag ttgtcacaga agaaacaagg aaaaaaataa
      121 gtaaaaagaa aaaaggaaga aaaatgtcag atgcagcaaa aaagaaaatg agtatagcac
      181 aaaaagggcg taaacaaaca caagaagcaa tagataaaac tgctacggca aaacaaaaaa
      241 gatgtgttaa aattgataaa gatacaaaag aaattataga aatatttgct tcattcagaa
      301 aagcagcgaa ttcattagga ttgattaaag caggtgattt aattagctgc gctagaggta
      361 aattaaaaac ttctcatggt tttatttgga aatatattga cactgaggac ttacatttaa
      421 ataaagatta tatttcaaaa ccaagaacaa gtggagcaaa gaaaataaat acatatgata
      481 aaactacaaa tgaattattt tttacttttt attctgcaac agaagcgaca aaaggattaa
      541 aactggaccc cagatgtatt gctaatattt gtaaagcagc ggttactaag gataaagttg
      601 cctatggcta taaattggaa tatgcctaaa taaaaaagag agagaatgat ttattctctc
      661 tcctaactac ttaacttaat ctaaaaatta aggagttacg tcattaaata ttgttttttg
      721 ttgaagtgcg caagccacga cgttctcgat cttccccgca aggtgaggtg caatgaaaac
      781 gagtcctaat tccatttttg tttgcgtatc aaaacagaaa ccattatgac gtgctgttga
      841 aagcttaata ccaccatcaa cccatccatg caatcctccc aatccaccag gaataggttc
      901 aagagcagga gctacagcac gataatctgc gctatcccaa aacaaggtag gaacaccttc
      961 tacacttaga ggaataaagt aaatatcgcc agtgtgagac acaggatttg tacctgtaat
     1021 agtcaatggc attgcatcat caagaataac catgtatgag cgaccattga ttgttaggcg
     1081 cattgtactt tgaagttgtt gacggattgt agcaatgccc atatcattac ttacaacagt
     1141 tacaccatta gtattagcaa cagccccacc agctagaaag ctaccgcaac catcaccaag
     1201 catttcacaa ggaagatatt taacaagtgc tgaccagtgt gttgggtgca ttacccaaac
     1261 ccattcaaca ttgttataac cataaagtga agctttgcca gttagagtat cttcaagttc
     1321 agtcatgtaa ccataaagac ctaaaccagt gttagcattt tgtccaccga tacaagttga
     1381 ttcaaagtct ttaatatctg aattcaattg tgttttattt gtaccagtta cgttggtctt
     1441 agtcccataa tcatcagcga ctaggaattc taaaccccaa aattgtttag ctccaccatt
     1501 agttgtattt agagcaacgt ttgttggatc accagtccag aattgcttta ggacttcatt
     1561 ttgtagacca cgagcaatta attggaattg tctacgtgta gcagcctgtg aaagtaagga
     1621 aagattatct tccatgccag caggaatacc agcacttacg ccacgaacat cgccaacaat
     1681 ataaaggtca gttgtgatac cttgatgata acgttgaata atcttatcaa gttcaagggt
     1741 ttgtgagcca agagagacac gacctttttc aacttcaatg aaagcagcag ttaagtttcc
     1801 aaccttttgt gggtcatcgc agggatagtc aggtaacgaa ccagaaggtt cagcgatgtc
     1861 agttagaaaa gcatacattg atttttgtgt attgcgtcta acgacaggaa tacgattagc
     1921 caaacttcgt ttaggtgtga tagtaacatt catcacagga cgttctaaca tacaattggc
     1981 gaacagtcca ccttcaacat acaaaagttg tgaagcgttt gtatttccaa cagctttctt
     2041 tagaacatca aattcatctg gattttgaac accagcagga acaaaaatgt ttcgtacttg
     2101 atcgttgaac gattttagta ctgtttcagc atatccactc atttaattta attctcctat
     2161 ataatatatt ttattcacga ttaaaaacca gctaagacat tatttgggtc atagcttttt
     2221 gtaacagttt tttcagttac agcagtcttt gcaatttctt cttgagaaac aataacttca
     2281 ccaacttctt gcttttcaac aacaccaaat tctttcttca tagcagcact aacagcagca
     2341 gcaggcataa agtcagatgc atttagaaat acattttgga atcttgcata agattttgta
     2401 gttgtatcag tagcaacagg ttcaaactta gcttcaagtt cagcaagttt agcttgtaaa
     2461 ggcgatacaa cgctttcatt atattccttc aaagctacaa gaacagcatc agtgataccc
     2521 ttttgaataa cttccataaa gttttcttgc gtatttgctg tttgtgtttc atctaccact
     2581 tctgttacag tttcagtttg agcaactaca gtttcttctt ttgtaactac ttcttctgta
     2641 acaacttcac tctttacttc atctttaact tccacgtttt caactccttt aaaaataatg
     2701 tttttttcaa ttgcagtttg attcaaagta tcaaccgcct taccaagaaa gtctaaagct
     2761 tgttcaggag cgccctgatt aatccaatca tttgctttct ttacgtttaa tttccccatt
     2821 tttttctcct aatttgtgct aaacgcagtt aataaatttg cagcattctt atgtggtaag
     2881 aatgtaaatt caaatggttt atattcgata attgtattat ctgcgtcacg tttaatatct
     2941 ttggtataaa taccctgaga catt
//
LOCUS       DAOQVQ010000292         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_39147_length_2959_cov_2.791322, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000292 DAOQVQ010000000
VERSION     DAOQVQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39147_length_2959_cov_2.791322"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PKN22_08565"
     CDS             <1..1449
                     /locus_tag="PKN22_08565"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HNS42796.1"
                     /translation="CKLEILKEVGEKVDSYKDKDLESILWLKADGSKPIRMNLAAAQG
                     QDKYDEPMHVMGVAENIKAPTGNGAELWKRMIGLRAKLVELTGSYKWGETNFTVSTTP
                     INDFTDNKDLTKKVTAMIDKNTKGNLKEDRQVLIDLYMMLTKKEKNKVHDQDGVHWVG
                     MTFDHVPLVAAIAALSSLEQEALSARALALAHWKGKVSTGEYSFNKIVPLAYGDLIAN
                     TGDSAHVSVMMAAFDSDNQPKVVVTEGATDYKLETADGQGKLGFRVAGGTEQVLKGTV
                     AIKNKSGVWKTAQWEHKIVIMKPSGAISLPELNVLYKGYDNKVSAVASGFDQTMLTGN
                     GVTVSKSGEFWIAKPGQVREASLSVSGKNSVTGKTQNLLTQKFRVSNLPDPELYWGAS
                     KNGDKGSRVETKLFAKYPPEIPLNAQFRIVDWEVSIPGAQGAPPRGTGGVLSPAASAL
                     IKQAKPGMIVSFLCNVVGPDGIKRKKAGAFKL"
     gene            1471..2484
                     /gene="gldN"
                     /locus_tag="PKN22_08570"
     CDS             1471..2484
                     /gene="gldN"
                     /locus_tag="PKN22_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNS42797.1"
                     /translation="MKSLFINIAAILAVGFSYAQIEDAGPVNSPNPGIIDGVVLKEHI
                     PTKRMIPYEFVREADVMWSKRVWQSIDLRERMNHALYYPFDEITPAGEWIKHGERWSL
                     WTVIRHNILNGKLTVFLPYNPNNQFGEWDGDQLKYALYPEQGLTYYTDSVYREKILYC
                     LGKLGPQSDIPLTDEYGDPLVITLPDGTQEFKYPPRDTIWYNSKNIIEYRIKEEWFFD
                     KERSVIDQRILAIAPVVYKMEVDAQGNESVGGTMELFWLYFPHCRFVFNNYFCYNDQN
                     DAQWMSFDDLFWKRRFSSYIIKESNGFDRKIETYRSGVDALRESEKIKEGIRTFEHDL
                     WDF"
     gene            2572..2835
                     /locus_tag="PKN22_08575"
     CDS             2572..2835
                     /locus_tag="PKN22_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42798.1"
                     /translation="MNKNLYFVPHDLTEVGDSALNYALHICHKVDAEIRILHLISDKA
                     EAIKASAKLDAIINNLSPENKEKVTKQIAIGYDDEIIILTFEN"
BASE COUNT         1007 a          421 c          594 g          937 t
ORIGIN      
        1 tgtaaattag aaattttaaa agaagtaggt gaaaaggttg attcatataa agataaagac
       61 cttgaatcta ttttatggtt gaaagcagat ggttctaagc ctattcgtat gaatttagct
      121 gctgcacaag gtcaggataa atacgatgag ccaatgcacg ttatgggtgt tgctgaaaac
      181 atcaaagctc cgactgggaa tggtgcagaa ttatggaaaa gaatgattgg cttgcgtgct
      241 aagttggttg aattaactgg atcgtataaa tggggtgaaa ctaactttac agtttcaaca
      301 actccaatta acgatttcac agacaacaaa gacttgacaa agaaagttac tgcaatgatt
      361 gataaaaaca caaaagggaa tttgaaagaa gaccgtcaag tgttaatcga tttgtacatg
      421 atgttgacga aaaaggaaaa aaacaaagta catgaccaag atggtgtaca ctgggttggt
      481 atgacttttg accatgttcc tttagtagca gcaattgcag cattgtcttc attggaacaa
      541 gaagcattat ctgctagagc cttagctttg gctcactgga aaggtaaagt ttctacaggt
      601 gagtatagct ttaataaaat tgttccttta gcttatggtg atttgattgc aaatacaggt
      661 gattcagctc acgtttcagt tatgatggca gcatttgact ctgataacca acctaaagtt
      721 gttgttacag aaggtgctac agattataaa ttagaaactg ctgatggtca aggtaaactt
      781 ggtttcagag tagctggtgg tactgagcaa gtattgaaag gtacagttgc aattaaaaac
      841 aaatcaggag tttggaaaac tgcacaatgg gaacataaaa ttgttattat gaaaccttct
      901 ggagcaattt cattgcctga attaaacgtt ctttacaaag gatatgacaa taaagtatct
      961 gcagttgctt ctggttttga ccaaacgatg ttaacaggaa atggtgtaac agtttcaaaa
     1021 agtggtgagt tctggattgc aaaaccagga caagtacgtg aggcttcgtt atctgtttct
     1081 ggtaaaaact ctgttacagg taaaactcaa aacttgttga ctcagaaatt tagagtatct
     1141 aacttaccag atccagaatt atactggggt gcatctaaaa atggagataa aggaagtaga
     1201 gtagaaacta aattgtttgc taaatatcca cctgaaattc cattaaatgc acaatttaga
     1261 attgtagatt gggaggtttc tatcccaggt gctcaaggtg ctccaccaag aggtacagga
     1321 ggtgtattat ctccagcagc gtctgcttta attaaacaag caaaacctgg aatgatagta
     1381 tctttcttgt gtaatgttgt tggtccagac ggaatcaaac gtaaaaaagc aggagcattt
     1441 aaattataat tgtattttaa ttcaaaagtt atgaagagtc tatttattaa catagcggca
     1501 attttagcag taggattttc ttacgctcaa attgaagatg caggacctgt taatagccca
     1561 aatcctggga ttattgatgg tgttgttctt aaagaacaca ttcctactaa gcgtatgatt
     1621 ccttatgagt ttgttcgtga agcagacgta atgtggagta aacgtgtatg gcaatcgata
     1681 gatcttcgtg aacgaatgaa ccatgcgttg tactatccat ttgatgaaat tacacctgca
     1741 ggtgaatgga ttaaacatgg tgaaagatgg agtttgtgga cagtaatacg tcacaacatc
     1801 cttaatggaa aattaactgt ctttttgcca tacaatccaa acaatcaatt tggtgagtgg
     1861 gatggtgatc aactaaagta tgcgttatat cctgaacaag gtttaacata ctatacagat
     1921 tctgtttata gagaaaaaat tctttattgt ttagggaaac ttggaccaca aagtgatatt
     1981 cctttaacag atgaatatgg tgatccttta gtaatcactt tgcctgacgg aacacaagag
     2041 tttaaatatc cacctcgtga tacgatttgg tataattcaa agaatatcat tgaataccgt
     2101 atcaaagaag aatggttttt tgacaaagag cgttctgtaa tagatcaacg tattttagca
     2161 atcgcacctg ttgtgtacaa aatggaagtg gatgcacaag gaaatgaaag tgtaggtgga
     2221 actatggaat tgttctggtt gtattttcca cactgtcgtt ttgtcttcaa taactatttt
     2281 tgttacaacg atcaaaatga tgcgcaatgg atgagttttg atgatttatt ctggaaacgt
     2341 agatttagtt catacatcat taaagaaagt aatggttttg acagaaaaat tgaaacttac
     2401 agaagtggtg ttgatgcgct aagagaatct gaaaaaatta aagaaggaat tagaactttt
     2461 gaacacgatt tgtgggattt ctaatctaat ttaataaaca tttaaggctc gaatttattt
     2521 cgagcctttt ttttgatttt ttttcttata tttgattaca tatttagctt tatgaataaa
     2581 aacttatatt ttgtacctca cgaccttaca gaagtaggtg atagtgcgtt gaattatgcc
     2641 ttgcatattt gtcacaaagt tgatgcagaa ataagaattc ttcatttaat ttctgataaa
     2701 gcagaagcta ttaaagcatc agctaaatta gatgcaatta ttaataactt gtctccagag
     2761 aacaaggaga aagtaaccaa gcaaattgct ataggttatg atgacgaaat tataatccta
     2821 acgtttgaaa attaaatttc ataaattttt aaaaaatgaa taaaaattta cattctgata
     2881 atatttgcac ctattttgtt gagtctcaaa tctaagtttt ggtaacctct gtcaatttgt
     2941 tcaatatttt caatttggc
//
LOCUS       DAOQVQ010000293         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_39792_length_2928_cov_3.223808, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000293 DAOQVQ010000000
VERSION     DAOQVQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39792_length_2928_cov_3.223808"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKN22_08580"
     CDS             <1..361
                     /locus_tag="PKN22_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNS42799.1"
                     /translation="TLVKMNYHIHTDAIKNNLIPPNIAKNKIPFIYASEADLLNVALF
                     GITAKEWREKNSDKKGNIRDYATLEQLVVLSNMESINALLIDQGLTQGERLIQLNKVA
                     ITQMKSLLEGKSIKKLK"
     gene            641..>2928
                     /locus_tag="PKN22_08585"
     CDS             641..>2928
                     /locus_tag="PKN22_08585"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS42800.1"
                     /translation="MTPLAFGQKMHIAGVVQDSSGMALPNALATAVRVKDSLLLEFAR
                     ADKDGKFVLDNIEMDTFMLTVSHPRFDDRSFYIVGSPSNLEIDIPSIKMATKSKQFDD
                     LVIYAYKDPIHFKGDTLVYIADSFKTGPNAVVEDLLKKLPGIEVDSDGKIKSQGQEIK
                     KVLVDGDEFFGSDPTIATKNLGAKAIETVEVYEKEDDGEFGSDNKIKVLDLKLKDDAK
                     KGYFGKVSGASDFGIADKKKPFYEGEFLFNHYKSTQKISVFGLGTNTPRSNFGWGDAN
                     KFGLENEDVSGSPFGNPFENTSPNGIPKTWKAGIYFTDKVGKKKNTKIGFNYTYYNKE
                     LQAYSGSRTQYFLTDTTYFTDDSTNNLTRSQSHIINFNVESQLDSLTKLTIKPSFRVD
                     QQLTDNNTLTQYLAEDNTSTLQTFANNKNKSLGLDGDLTAKLNRRFKKPKREIELDYY
                     FNYIDNKTKGSILTRTESPLFPAVNDTTDQSKINYNSTMVNNAFASYTEPFGKYLKGT
                     IMYNYRNELLKQNRETNNFVNGSYSDLDSTLSNQFETKRNEHKAGLKLQFDKDKHMAF
                     VRVDVRNVDINNVNLFTNNSIHQNISNILPSVRYQYRASMSKQLRITYSTNSEQASIT
                     DLQPVPDNSNPNRIKVGNPNLRPNYSHELNAFFNSWNPINSGFIWAGMNASYTNNAFA
                     TSTTYDAFGRMNIQTVNVDGNFNSYIYGGGGIPLKGRTIQLHPNLLAGYSHFTNFINN
                     QKNVTDNWLLGGGGEFKFRWDS"
BASE COUNT         1034 a          488 c          507 g          899 t
ORIGIN      
        1 aactttggta aagatgaact atcacataca cacagatgct ataaaaaata atctcatacc
       61 accaaatata gctaaaaata aaataccttt tatttatgct tctgaagctg accttttaaa
      121 tgttgcgctt tttggaataa cggctaagga atggagagaa aaaaattctg ataaaaaagg
      181 aaacattaga gattatgcaa cattagaaca attagttgtt ttgagtaata tggaaagcat
      241 taacgctttg ttaattgatc aaggattaac tcaaggagaa cgacttattc aacttaataa
      301 agttgcaatt actcaaatga aatctttgct tgaaggtaaa tccattaaaa aattaaaata
      361 gttgggcaac aaagattaaa cacaaaataa caaagtattt tcttttaaca acatgaagta
      421 acagtagttt aaatttgttt tgatgctgtt ctatttcata tccaaaaacc attcattccc
      481 caataatttc ttcactttat ccttttattt ttccattcaa ccagaaatac agccactagt
      541 aatttctttt ttttggttta gtgtcaatta tttacatttg ttgtgttaat catccccatt
      601 tatgttaaaa aaacttttat ttgcttttat actagcaatt ttgactcctc ttgcatttgg
      661 acaaaaaatg cacattgcag gtgttgtgca agatagtagt ggtatggctt tgcccaatgc
      721 attggcaacc gctgttcgtg ttaaagactc gttgttgtta gaatttgcac gcgccgataa
      781 agacggaaaa tttgtgttgg ataatattga aatggatact ttcatgctta ccgtttcaca
      841 tccaagattt gatgatcgtt ctttttatat agtgggaagt ccaagcaatc tggaaattga
      901 tattccaagt attaaaatgg ctacaaaatc aaaacaattt gatgatttgg tgatttatgc
      961 ctacaaagac cctatccact ttaaaggtga taccttggtg tatattgccg attcgtttaa
     1021 aactggtccc aacgcagtag tggaagattt attaaaaaaa cttcctggta ttgaggtaga
     1081 tagtgatgga aaaataaaat cgcaagggca agaaattaaa aaagttttgg ttgatggtga
     1141 tgagtttttt ggttctgatc ctaccattgc aacaaaaaac ttgggtgcaa aagccataga
     1201 aactgtagaa gtctatgaaa aggaggatga tggcgaattt ggcagcgaca ataaaatcaa
     1261 agttttggat ttaaaactca aagacgatgc taaaaaaggt tattttggta aagtgtcggg
     1321 tgcatccgac tttggaattg ccgataagaa aaaaccattt tatgaaggtg aatttttatt
     1381 taaccattat aaaagcacgc aaaaaatatc tgtttttggt ttaggaacca acactcctcg
     1441 ctctaatttt ggttggggcg atgccaataa atttggatta gaaaatgagg atgtaagcgg
     1501 aagtccattt ggaaatcctt ttgaaaatac ctcgcctaat ggaattccaa aaacttggaa
     1561 agcaggtatt tacttcaccg ataaggttgg taaaaagaaa aacacaaaaa ttgggttcaa
     1621 ctatacctat tacaacaaag aattgcaagc ctattctggt agtagaacac agtatttctt
     1681 aacagatacc acttatttta cggatgattc taccaataat ttaacacgta gtcagagtca
     1741 tattatcaat tttaatgtgg aatcacaatt ggattctttg accaaattaa cgattaagcc
     1801 atcgtttaga gtggatcaac aattaacaga taacaacaca ttaacccagt atttggcaga
     1861 agacaacact tcaacattgc aaacatttgc caataacaaa aacaaatcgt tgggattaga
     1921 tggcgattta actgcaaaat tgaacagacg atttaaaaaa ccaaaacgtg agatagagtt
     1981 agactattat ttcaattaca ttgacaataa aacaaaaggt tctatcttaa ctcgcaccga
     2041 atctccgttg tttcctgcgg tgaacgatac tactgatcaa tccaaaatta actacaacag
     2101 cacgatggtt aacaatgctt ttgcatcata taccgagcct tttggcaagt atttgaaagg
     2161 aactatcatg tataattaca gaaatgaatt gttgaaacaa aaccgcgaaa ccaacaattt
     2221 tgtaaacggt tcctattcag atttagactc tacgttatcc aaccaatttg aaacaaaacg
     2281 caacgagcac aaggcaggtt taaaattaca atttgacaaa gacaaacaca tggcatttgt
     2341 gcgtgtggat gtgagaaatg tggacataaa caacgtgaat ctgtttacaa acaattccat
     2401 tcatcaaaac atttccaata ttttgccaag cgtacgttat caataccgtg caagcatgag
     2461 taaacaattg cgcataacct atagcaccaa ttctgagcaa gcttctatta ctgatttaca
     2521 acctgttccc gacaattcaa atccaaacag aattaaagtt ggtaacccca atttgcgtcc
     2581 aaattattcc catgagttga atgcgttttt caattcttgg aatccaataa acagcggttt
     2641 tatatgggcg ggaatgaatg cttcatacac caacaatgct tttgcaacat ccacaaccta
     2701 tgatgctttt ggtagaatga acattcaaac tgttaacgta gatggcaact tcaacagcta
     2761 tatttatggt ggtggaggta ttccgttaaa aggacgcacc attcagttgc accctaattt
     2821 gttagcaggt tatagtcatt ttactaattt tattaataac cagaaaaacg taaccgataa
     2881 ctggttgctt ggtggaggtg gtgaattcaa atttagatgg gattcatt
//
LOCUS       DAOQVQ010000294         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_39819_length_2926_cov_3.921282, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000294 DAOQVQ010000000
VERSION     DAOQVQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39819_length_2926_cov_3.921282"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKN22_08590"
     CDS             complement(<1..820)
                     /locus_tag="PKN22_08590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNS42801.1"
                     /translation="MKNVKEAANFIWSIADLLRGDYRQSDYGKVILPLTVLRRLDCVL
                     DATKAKVLKEYEQMKSSNIQNLDPILNRAAGLKFHNHSAFDFNKLIADPNNIAANLRN
                     YINGFSEEAREIIEQFEFDNQITKLDDNNLLFMVMKRFQEVDLHPKTVSSMDMGYMFE
                     ELIRKFAELSNETAGEHFTPREVIKLMVNILFMDDRQALTQKGIVKTIYDCCAGTGGM
                     LSVAEEYLNELNPDARLEVFGQELNPESYAICKSDMLIKGQNPSNIKKGNSISED"
     gene            complement(924..2375)
                     /locus_tag="PKN22_08595"
     CDS             complement(924..2375)
                     /locus_tag="PKN22_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNS42802.1"
                     /translation="MQTFRKNSWILFWSVCYLVIASILLWKRQEFLVLFPIGLLAVFA
                     AIYYTEQTFLALAFFTPLSVNIEQYTDSFGLFIPTEPLLFGFLLLFVMQQFLKNNIIR
                     EVWKSPIVWAVIFYLFWMLVASILSTHPIVSLKYLLARIWLIVPLLFFGPLIFAKISN
                     RTTFLWLFSIGMTIAMIYIILNHAQYSFGEKESHWVSWPFFKDHTIYGATIALQTPLV
                     IGLYFSKKYPPLTQVVLIGMIIVNLLGLYFSYTRAAWLSVIGAVGVLLLIRFKIKFSW
                     IALVVVIVGTVVAVSWTQINHYLEQNRYEHTTEDFGERMQSATNVTTDASNLERINRW
                     QCAVDMFLEKPMTGFGPGTYAFEYARFQRPENLTIISTNFGDLGNAHSEFLGALSEMG
                     IFGLLAVIALVATIFYQSITLYVRWPHEEKENRILLLSMILSMTTYFIHAFLNNFLDT
                     DKAAVPIWGMCAIVIAMQRELNERGKEKANTSN"
     gene            complement(2376..>2926)
                     /locus_tag="PKN22_08600"
     CDS             complement(2376..>2926)
                     /locus_tag="PKN22_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNS42803.1"
                     /translation="EKAAAMANRIVDLIDTVKNQMIEQRTVEAFKIAARKKDMLQSEL
                     KQIDIKIDSLANLGVMNVDARAGLYQALVDAKSPAEKTEIKQKLLINEKDGALYDKLE
                     HFRREKITAYELFMAIYEQAESDAHTQLNHKFVVERAVVPDKKDKPKRMIVVLLGTMG
                     ATIFSIFALLVRDKIRELKKIA"
BASE COUNT          921 a          593 c          534 g          878 t
ORIGIN      
        1 tatcctcaga aatactgttt ccttttttga tattggacgg attttgtcct tttatcagca
       61 tatccgattt acaaatagca tacgactctg ggttgagttc ttgcccaaac acttccaaac
      121 gcgcatcggg gttcaattca ttcaaatatt cttcggcaac agacaacatt ccacccgttc
      181 cagcacagca atcgtaaatg gtttttacaa ttcctttttg ggtgagcgct tgacggtcgt
      241 ccataaacaa aatattcacc atcaatttaa taacttcgcg aggagtaaag tgttctccag
      301 ccgtttcgtt ggatagttcg gcaaattttc ggataagttc ttcaaacata tagcccatat
      361 ccatagacga cactgttttg gggtgtaaat ccacttcttg aaagcgtttc atcaccataa
      421 acaacaaatt gttgtcatcc agcttcgtaa tttgattatc aaactcaaac tgttcaataa
      481 tttctcgtgc ttcttccgaa aaaccattga tgtagttacg caagtttgca gcaatgttat
      541 ttgggtctgc aattagttta ttgaaatcaa aagcactgtg gttgtgaaat ttcaatccag
      601 ccgctctgtt caaaatagga tctaaatttt gaatattaga tgatttcatt tgttcgtatt
      661 ctttgagaac ttttgccttg gtagcatcta aaacacaatc caagcgtctt aataccgtta
      721 gcggaagaat tacttttcca taatcacttt gtcggtaatc tccacgaagt aggtcggcaa
      781 tagaccaaat aaaattagca gcttctttaa cgtttttcat tcttttatta atcagttgag
      841 tattgaattt tctatgaaag tgactaattt aagaataaag tggaaatgcg caaactatta
      901 tgcgttcaat gtaaaaatga aaattagttg gaggtgtttg ctttctcctt ccctctttcg
      961 ttcaattcac gttgcatagc aataacaata gcacacatgc cccaaattgg aacggccgct
     1021 ttgtcggtgt ctaaaaagtt gttcaaaaag gcgtgaatga aataggtagt catggataaa
     1081 atcatgctta aaaggaggat gcggttttct ttttcttcgt gtggccagcg cacatacaag
     1141 gtgatggact ggtagaaaat agtagccacc aatgcaatga cagccaataa accaaaaata
     1201 cccatttctg atagtgctcc taaaaattcg ctgtgggcat tgcctaaatc gccaaaattg
     1261 gtggaaataa tggttaaatt ttcgggacgt tgaaagcggg catattcaaa agcataggtt
     1321 ccgggcccaa atccggtcat tggtttttcc aaaaacatgt caacggcaca ttgccaacgg
     1381 ttgatgcgct ctaagttgga ggcatctgtg gtaacattgg tagcagattg catgcgttct
     1441 ccaaaatctt cggtggtatg ttcgtaacgg ttttgttcaa gataatggtt gatttgtgtc
     1501 caactaacag ccaccactgt tccaacaatt actaccacaa gggcaatcca actaaattta
     1561 attttaaaac gaatgagcaa caacacaccc acagcaccaa taacgctcaa ccacgctgca
     1621 cgggtatatg aaaaatacaa gcccaacaag ttaacgatga tcattcctat caatacaact
     1681 tgggttagag gtggatattt tttggagaaa tacaaaccaa ttaccaacgg tgtttgcagg
     1741 gcaatggtgg cgccataaat ggtatggtct ttaaaaaatg gccaactcac ccaatgcgat
     1801 tctttttcac caaaactgta ttgtgcatgg ttaagaatga tgtaaatcat agcaatggtc
     1861 attccgatag aaaacagcca taaaaacgtt gttctgttgc ttattttagc aaaaatgagc
     1921 ggcccaaaaa acaaaagtgg cacaatcaac caaattcttg ccaacaaata tttaagagaa
     1981 acaattggat gggtactcaa aattgaggcc accaacatcc aaaagagata aaaaataacc
     2041 gcccaaacaa tgggtgattt ccaaacttcg cgaataatgt tgttttttaa aaactgctgc
     2101 atcacaaaga gcaataaaaa gccaaacaag agcggttcgg ttggaatgaa cagtccaaaa
     2161 ctatcggtat attgttcaat attaaccgat agtggcgtaa aaaaagccaa ggctaaaaat
     2221 gtttgttcgg tgtagtaaat tgctgcaaaa acagccaaca aaccgatggg aaaaagcact
     2281 aaaaattctt gtcgcttcca caacaaaata gaagcaatta caaggtaaca aacgctccaa
     2341 aatagtatcc agctattttt acggaaggtt tgcatttagg caattttctt aagttctctg
     2401 attttatcgc gcaccaacag ggcaaagatg gagaaaatgg ttgcgcccat tgtacccaac
     2461 aacaccacaa tcatgcgttt tggtttgtct tttttatcgg gaacaacagc tctttcaacc
     2521 acaaatttat ggttcaactg cgtgtgtgca tccgactctg cttgttcgta gattgccata
     2581 aacaactcgt aggctgtaat tttttcgcga cggaaatgct ccaatttatc gtaaagcgca
     2641 ccatcttttt cgttgataag caatttttgt ttgatttctg ttttttctgc tggactttta
     2701 gcatccacca acgcttgata caatcccgca cgagcatcca cgttcatcac acctaaattt
     2761 gccaagctat caattttaat atcaatctgt tttaattcgc tttgcaacat gtctttttta
     2821 cgcgctgcaa ttttaaaagc ctcaacggta cgttgttcaa tcatttgatt tttaaccgta
     2881 tcaattaaat caacaatacg attggccatt gcagcagctt tttctg
//
LOCUS       DAOQVQ010000295         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_40099_length_2913_cov_3.512246, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000295 DAOQVQ010000000
VERSION     DAOQVQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40099_length_2913_cov_3.512246"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKN22_08605"
     CDS             <1..1113
                     /locus_tag="PKN22_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS42804.1"
                     /translation="GQVLQKVKISGGGRCNVTNACDDIEAFSKFYPRGERQLKRAFYQ
                     FNQQSTIDWFASKGVQLYAQPDNRMFPTSNNSQTIIDCLMSECKTLGIELFLSSNIES
                     IQKKDDLFWLNTPTQTFIASKVIVATGGSAKESGFDWLKNLGFRIATPVPSLFTFNMP
                     KNPICELMGNSVAATTVKIEGTKLSSSGPLLITHWGMSGPAILQLSAWGARTLAEKNY
                     LFSIFVNWLHTTTEDELRHQFNQQKKANAEKLLINANPYAISTKISHFLLNKYGISSQ
                     LRWKELEGKNCNKLIQALLFDKYDVAGKTTFKEEFVTCGGVQLNEIDFQTMEAKKIKG
                     LYFAGEVLDIDGITGGFNFQAAWTTGYIAGINAKIN"
     gene            1131..1727
                     /locus_tag="PKN22_08610"
     CDS             1131..1727
                     /locus_tag="PKN22_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42805.1"
                     /translation="MNRIFSLICIMHFVEPHFGWRGYYTAEDDQLSPFYGRQYSEFEF
                     ENSVYNYCIHPQWDHFGSETLYLKLLFADYDDGYAIIEFIGEWNDAIENDIMVLKRDF
                     IDSLIHEGITKFILIGENVLNFHASDDSYYEEWIEDIEDGWVAMVNFHDHVSREMNSA
                     NIDHYFLSGGQLDDVEWRTFQPHQVFRFISKIVENRLN"
     gene            complement(1810..>2913)
                     /gene="pepD"
                     /locus_tag="PKN22_08615"
     CDS             complement(1810..>2913)
                     /gene="pepD"
                     /locus_tag="PKN22_08615"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HNS42806.1"
                     /translation="GVASIMAILASKNIPHPAIEAMFTIDEETGMTGALKLDGSNFQG
                     QILLNLDSEDDDELAIGCAGGIDTNTKYYYQEESVKPDAVFLTIAIRGLLGGHSGMDI
                     DKGRGNANKLMNRILYHLISLHEFQLISIDGGSLRNAIPRESTAVIAINKSILADVKE
                     AISSISAILLHEFKTIEKGLTINVEEKGEGDKAVTHEDILKILNVIYTMPNGVFRMSP
                     DIEGLVETSSSLARVIVNDGTFITQSLQRSSVDSSKADIANAIRSGFELAGMAVVQNG
                     DYPGWQPNMDSPILKLMHKMYVERFNEEPHVRACHAGLECGILGIHLPGVDMISFGPN
                     IRAPHSPDEKVQISSVQKFWGFLLQVLNEIPQK"
BASE COUNT          928 a          568 c          536 g          881 t
ORIGIN      
        1 ggtcaggtat tgcaaaaagt gaagataagt ggcggtggaa gatgtaatgt taccaatgct
       61 tgcgatgata tagaagcgtt ttcaaaattt taccctcgtg gcgaacgaca gctaaaacga
      121 gcattttatc aattcaacca acaatcaact atcgattggt ttgcaagcaa aggagtgcaa
      181 ctttatgcgc aacccgacaa ccgtatgttt ccaacaagca acaattctca aacaatcatt
      241 gattgcttga tgagcgaatg caaaacattg gggattgaac ttttcttaag ctctaatatt
      301 gaatccatcc aaaaaaagga cgatttattt tggttaaaca ctcccacaca aacatttata
      361 gcctccaagg taattgttgc aactggtggc agcgcaaaag aaagtggttt tgattggcta
      421 aaaaacctcg gtttccgtat cgccacgcct gtcccctctt tgtttacgtt caacatgcct
      481 aaaaatccaa tatgcgaatt gatgggcaac agtgtggcag caaccaccgt aaaaattgaa
      541 ggcaccaaac tcagctctag cggtccgttg ttgattaccc actggggaat gagcggtccg
      601 gcaattctac aactatccgc ttggggagca cgcaccttgg ctgaaaaaaa ctatctcttt
      661 tcgatttttg taaattggct ccatacaaca accgaagatg aattgcgcca tcaattcaac
      721 caacaaaaaa aagcaaatgc agaaaaatta cttatcaacg ccaatcctta cgctatttca
      781 actaaaattt cgcatttctt attgaataaa tatggaattt catctcaact tcggtggaaa
      841 gaattggaag ggaaaaattg caataaactg attcaggctt tgttgtttga caaatacgat
      901 gtagccggaa aaacaacttt taaagaagaa tttgtaacct gtggcggtgt tcaactaaac
      961 gaaattgatt ttcaaaccat ggaagcaaaa aaaattaaag gactttattt tgctggcgag
     1021 gttttggata ttgatggaat aacgggaggt ttcaattttc aagctgcttg gacaacaggt
     1081 tacattgcag gaataaatgc caaaatcaat taattggtgt cgtatgaaat ttgaatcgta
     1141 ttttttcgtt gatttgcatt atgcattttg ttgaacctca ttttggatgg cgcggttact
     1201 atactgcaga agacgatcag ttatctccgt tttatggtcg ccagtacagt gaatttgaat
     1261 ttgaaaacag cgtttacaac tattgcatac atccgcaatg ggatcatttt ggttcagaaa
     1321 ctttgtacct caaattattg tttgcggact acgacgatgg ctatgcaatc attgaattta
     1381 ttggtgaatg gaacgatgcc attgaaaatg acatcatggt attaaaacgt gattttattg
     1441 atagtttaat tcacgaaggc atcacaaaat ttattctcat tggcgaaaac gtattgaatt
     1501 ttcatgcttc agacgatagt tattacgaag aatggataga agacattgaa gacggttggg
     1561 tggcgatggt caatttccac gaccacgtga gcagagaaat gaacagcgct aatattgatc
     1621 attattttct ttctggcggt cagttagacg atgtagaatg gcgcacgttt caacctcatc
     1681 aagtttttcg atttatttca aaaattgttg agaaccgatt aaattaaatc attaacaaac
     1741 aaataatgcg taaactttca catttacgca ttattattca aatccaccta aaggtgaaaa
     1801 tacattagtt tatttttgag gaatttcgtt caacacttgc aacaaaaagc cccagaattt
     1861 ttgaacggat gaaatttgta ctttctcgtc tggtgaatgt ggcgcacgga tgtttggtcc
     1921 aaacgaaatc atatccactc ctggtaaatg aattcccaaa ataccacatt ccaatcccgc
     1981 gtggcaagca cgtacatgcg gttcttcgtt aaaacgttct acatacattt tatgcatcaa
     2041 tttcaaaata ggcgaatcca tattgggttg ccatcctggg taatctccat tttgaacaac
     2101 agccatacca gccaattcaa aaccagaacg aatggcgttg gcaatatctg ctttagatga
     2161 atcaacacta ctgcgttgta acgactgcgt gatgaaagtt ccatcgttga caatcacacg
     2221 tgccaaactt gatgaagttt caactaatcc ttcaatatcc ggactcattc tgaaaacacc
     2281 gttgggcatg gtgtaaataa cgtttaaaat ctttaatata tcttcatgag ttaccgcttt
     2341 gtcgccctca cctttttctt caacattgat ggttaaccct ttttcaatgg ttttaaattc
     2401 atgcaataaa attgcagaaa tggatgaaat tgcttcttta acatctgcca aaatgctttt
     2461 attaatggca atcacagcag ttgattcacg tggaatagcg tttcttaaac ttccaccatc
     2521 aatggaaatc aattgaaatt cgtgtaaaga aatcaaatgg tataaaattc tgttcatcaa
     2581 tttattggcg tttccacgtc ctttatcgat gtccattcca gaatgtccac ccaacaaccc
     2641 tctgatggca attgtaagga aaacagcatc tggtttcacg ctttcttctt ggtaataata
     2701 tttggtattg gtatcaattc caccagcaca accaatggct aattcgtcgt cgtcttcaga
     2761 atctaaattc aataaaattt gtccttggaa attagaacca tccaacttca aagctcccgt
     2821 catacctgtt tcttcatcaa ttgtaaacat ggcttcgata gcaggatgag gaatattttt
     2881 tgatgccaaa atcgccataa tagacgctac acc
//
LOCUS       DAOQVQ010000296         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_40199_length_2908_cov_3.970908, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000296 DAOQVQ010000000
VERSION     DAOQVQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40199_length_2908_cov_3.970908"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKN22_08620"
     CDS             <1..856
                     /locus_tag="PKN22_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42807.1"
                     /translation="TQFNVDVFNKIVAGTIDIPNTTVKLILENGIKMDGSGLIKHLKS
                     TNNQGNVVYLSGGNVGTTFTIDQPTGSFSTLVPSQRIIEFNSGNSNIEAFIENLGVKN
                     ELAYGIKINPWGNISGGWNEVFPQSKLKMTLEAQMPLNIKMDNLTIRDTFAFQLSQKK
                     DKSHIESGVLQLDLDNAFPFEGKIKLLFVDENGAVIETVNASQPIVSSLYGEQNTNGL
                     WHKTSTVEVPLSIVAVDKLNSTKNIIVEAIFNTPDPNTSSNQMVGIPVNAYLGIKAKA
                     KIQLKMKI"
     gene            1009..>2908
                     /gene="mutS"
                     /locus_tag="PKN22_08625"
     CDS             1009..>2908
                     /gene="mutS"
                     /locus_tag="PKN22_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNS42808.1"
                     /translation="MKQYNSIKSKHPGTLLLFRVGDFYETFGEDAIKTSKILGIVLTK
                     RGNGSESETALAGFPHHSLETYLPKLVRAGQRVAICDQLEDPKMAKGIVKRGVTELVT
                     PGVSFNDQVLEQKKNNFLCAIHFTKAGHGIAFIDVSTGEFLLAQGNDEYIDKLIQGFE
                     PKEIIFQKNKSKEFDALFGTKFYHFRLDDWVFTPDFALENLNKHFNTKSLKGFGVENM
                     DLGIVAAGAILHYLSETQNDKLGHITAVSRIEEDKYVWLDQFTLRNLELISSPHPEAT
                     TLSDILDKTQTAMGGRMMKRWMVLPLKNATNVQERLEAVNYLKNKEEIGEQLITCLRE
                     IGDLERLISKVAVGKVNPKEVIQLKRTILQIAPIKALLQATDVLLLDKIVDQLNPCQK
                     LIDLIENTLLDDPAIAIGKGRVIADGVNQELDEIRTLLNSSKDYLEDIQERESQNTGI
                     PSLKIAFNNVFGYYLEVRNTHKDKVPADWIRKQTLTSAERYITEELKEYETKILGAEE
                     KIGALEAQIYQNLLLSLTDFIQSVQHNAFLIAQLDCLAAFAVVAQANNYVQPTINDSF
                     VIDIKEGRHPVIEQKLPIGESYIANDVFLDNKSQQIIMITGPNMSGKSAILRQTALIC
                     LMAQMGSFV"
BASE COUNT          978 a          502 c          584 g          844 t
ORIGIN      
        1 gacacagttc aatgtggatg tgtttaataa aattgttgca ggcaccattg atattcctaa
       61 cacaactgtt aaactaattc tagaaaatgg aattaaaatg gacggctcgg ggttgattaa
      121 acaccttaaa agcacgaata atcaaggaaa tgtggtttat ctttcgggag gaaatgttgg
      181 aacaaccttt acaattgacc aaccaacggg tagtttttca acattggtac catctcaacg
      241 catcatagaa tttaactctg gaaatagcaa tattgaagcg tttattgaaa atctaggtgt
      301 taaaaatgaa ttggcttatg gaattaaaat caatccgtgg ggcaacattt ctggaggttg
      361 gaacgaagtg tttccgcaaa gcaaattaaa aatgacattg gaagcacaaa tgccattgaa
      421 cataaagatg gacaacctta ccattcgtga tacgtttgct tttcaactga gtcagaaaaa
      481 agataaatca cacattgaaa gtggtgtgct gcaattggat ttagataatg cttttccgtt
      541 tgaaggaaaa attaaacttc tttttgtgga cgaaaatggc gctgtaattg aaacagttaa
      601 tgctagccaa cctattgttt cttcgttgta tggtgagcaa aatactaacg gattgtggca
      661 caaaacaagc accgtagaag ttccattgtc aattgttgct gtggataaac tcaattcaac
      721 caaaaacatt attgttgaag ctatttttaa tacgccagat cccaacacgt catccaatca
      781 aatggtggga attcctgtaa atgcgtattt gggaatcaaa gccaaggcaa aaattcagtt
      841 aaaaatgaaa atttagtttt ttcgttaatt acttcttgat tttgttaata agaacttgca
      901 caaatgtgta aggtttccta ttgctttcat taattttacc aaccacaatt ctaaatcaat
      961 agcgtgagcg aaaacgacga caaaaagacg gccaatgtta cgccattaat gaaacaatat
     1021 aacagtatca agagcaaaca tccgggtact ttactcttgt ttcgtgtagg tgatttttat
     1081 gaaacttttg gagaagatgc catcaaaaca tctaaaattt taggcattgt tcttactaaa
     1141 cgaggaaatg gaagtgaatc tgaaacggct ttggcaggtt ttcctcatca ttcattggaa
     1201 acgtatttgc ctaaattggt gcgtgctggt caacgagtgg ctatttgcga tcaattggaa
     1261 gatcctaaaa tggccaaagg aattgtaaaa cgtggtgtta ccgaactagt aactcctggc
     1321 gtttcattca acgatcaagt gctggagcaa aagaaaaaca attttttgtg cgccattcat
     1381 tttaccaagg ctggacatgg aattgcattc attgatgttt ctacaggcga atttttgttg
     1441 gcgcaaggaa acgacgaata catcgataag ctcattcaag gatttgaacc caaagaaatc
     1501 atttttcaaa aaaataaatc caaagaattt gatgcgcttt ttggtacaaa attctaccat
     1561 ttccgtttag atgattgggt ttttacaccc gattttgcac ttgaaaattt gaataagcac
     1621 ttcaatacca aatcgctcaa aggttttggt gtagaaaata tggatttggg aattgttgcc
     1681 gctggagcca ttttgcatta tttatctgaa acacaaaacg ataaattggg gcacattacc
     1741 gctgtttctc gtatcgaaga agataaatac gtgtggttag accaatttac cttgcgtaat
     1801 ttagaattga tttcgtcgcc acatcccgaa gcaactacgc tcagcgatat tttggataaa
     1861 acacaaaccg ccatgggtgg gcgcatgatg aaacgttgga tggtgttgcc actaaaaaat
     1921 gcaaccaatg tgcaagaacg cctcgaagcg gtgaattatc tgaaaaacaa ggaagaaatt
     1981 ggtgagcaac tcatcacctg tttgcgtgaa attggcgatt tagaacgttt gatttctaaa
     2041 gtggcagtgg gaaaagtaaa tccaaaagaa gtcatccaac tcaaacgaac gattcttcaa
     2101 attgctccca tcaaagcatt gttgcaagca accgatgtgt tgttgttgga taaaattgta
     2161 gatcaactca atccgtgtca aaaactcatt gatttaattg agaatacttt gctcgatgat
     2221 cctgcaattg caataggcaa ggggagagta atagcagatg gtgtgaacca agaattggat
     2281 gaaattcgca cgcttcttaa ctccagtaaa gattatttgg aagatattca agaacgcgaa
     2341 agtcaaaata caggaattcc aagcttgaaa attgcgttca ataacgtttt tgggtattat
     2401 ttggaagtta gaaacaccca caaagacaaa gtgccagccg attggatacg caaacaaaca
     2461 ttgaccagtg ccgaacgcta catcaccgaa gaattaaaag agtatgaaac aaaaattttg
     2521 ggagctgaag aaaaaattgg tgctttagaa gcgcagattt accaaaattt attgctttca
     2581 ctcaccgatt tcattcagtc ggttcaacac aatgcctttt taattgctca attggattgt
     2641 ttagcagctt ttgcggtggt tgctcaagca aacaattacg tgcaacccac catcaacgat
     2701 agttttgtaa ttgacattaa agaaggtcgc cacccagtta ttgaacaaaa attgccgatt
     2761 ggcgaaagtt acattgctaa cgatgtgttt ttggacaata aatcccaaca aatcatcatg
     2821 attacgggac caaatatgag tggtaaaagt gctattttgc gtcaaacagc attgatttgt
     2881 ttaatggcgc aaatgggaag ttttgttc
//
LOCUS       DAOQVQ010000297         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_40250_length_2905_cov_3.662105, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000297 DAOQVQ010000000
VERSION     DAOQVQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40250_length_2905_cov_3.662105"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..514
                     /locus_tag="PKN22_08630"
     CDS             98..514
                     /locus_tag="PKN22_08630"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNS42809.1"
                     /translation="MNSFVSRYAIIATVLVLVSSCTSTEPVEIRVNKDQCANCKMTIT
                     ELNYASELVTSKGKTFAFDDIKCMDYFQNNHEDEAIKNAISYVIDFNTLEFVKKNEAF
                     LIQGGAIKSPMNGNIQAFKNKEDAQNAAQKLNAQPL"
     gene            524..1762
                     /gene="nosD"
                     /locus_tag="PKN22_08635"
     CDS             524..1762
                     /gene="nosD"
                     /locus_tag="PKN22_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNS42810.1"
                     /translation="MLRFLLAFICFFPLISFGKTLKVGKNEKYKTIVQAINNAVNGDT
                     VLVFSGIYKEGNINVTKSIALIGKDFPVIDGEHKYENISFKANKIVLKGFKIINSGED
                     EVKNVGAVRLYDSHYSIIEDNIFDNNYFGITIQRGYKCVVRNNKIVYRFKRSQEKIGD
                     GIHIWNSSHLKILNNYIEGTKDGIYLEKASHVIVDGNYSTRNLRYGLHFMFSNDNSYS
                     NNTFNNNYAGVAVMYSMHVDMYKNKFINNWGDGAYGVLLKEISFSKIEGNIFDNNTAA
                     VYMDGATKIDFFKNKFANNGWGVKINANCMENKFAYNHFENNTFDMSTNGSLVMNTFN
                     KNYWDKYQGYDLNKNKIGDIPYHPLSLYSVLSDKNPTIMLLYRSFIVDLLDRIEKMIP
                     SFTPDNFIDKFPLMKSPIKD"
     gene            1769..2485
                     /locus_tag="PKN22_08640"
     CDS             1769..2485
                     /locus_tag="PKN22_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS42811.1"
                     /translation="MITIKNLTKKFNKFIALNNVNLSFSQGEAIALIGPNGCGKTTLI
                     KSILGLNVVKSGTIEVLNNDISNNYTYRENIGYMPQIGRYPENMTIEQTINMIIEARN
                     VPKNQLDLELLEAYQLESIYSKKMSTLSGGTTQKVSAVLAFLFHPKIIILDEPTAGLD
                     PLAAEILKNKIIKEKNNGKLIIITSHLLSELDDIISQVVFIKEGTVIFHQSVDDLKKN
                     TASSKISEAIINVLKSNNHE"
     gene            2478..>2905
                     /locus_tag="PKN22_08645"
     CDS             2478..>2905
                     /locus_tag="PKN22_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42812.1"
                     /translation="MNRIIRFIVFDIIKNKIVLFYTILLFIISWSVLGIETNYTKSIL
                     SLLNVVLLVVPLVSVVFSTIYVYNSNQFIELLLSQPVPRSKIWLSMFLGLSSALSFAF
                     IIGCGLPILIYSSIEIGFSLVISGVFLSIIFTAIAMLAAI"
BASE COUNT         1000 a          452 c          493 g          960 t
ORIGIN      
        1 cataccctaa cataggtgga tggattatgt tttccattgc tattgtgcta atttttctta
       61 gctttagaga atataaatta tcaaaaaaat aaaaagtatg aattcatttg ttagtcgtta
      121 tgcaattatt gcaactgtat tggtattggt aagtagttgc acttcaaccg aacctgtgga
      181 aattcgtgta aataaagacc aatgtgctaa ttgtaaaatg acgattactg agcttaatta
      241 tgcttcggaa ttggtgacat caaaaggtaa gacttttgct tttgacgata ttaaatgcat
      301 ggattatttt cagaacaatc atgaagacga agccattaag aatgcaatta gctatgttat
      361 tgattttaac acactcgaat ttgtaaagaa aaacgaagcg tttttaatcc aaggtggcgc
      421 tattaaaagt ccaatgaacg gtaatattca agcatttaaa aacaaagaag atgcacaaaa
      481 tgctgctcag aaattgaatg cacaacctct ataattaaat gcaatgttgc gatttttgtt
      541 agcttttatc tgttttttcc ctttgatttc ttttggaaaa acactgaaag ttggaaaaaa
      601 tgaaaaatac aaaactattg ttcaagccat taataatgct gtcaatggtg atactgtgct
      661 cgttttttca ggaatttaca aagaaggaaa tatcaatgta accaaatcta tcgcattgat
      721 tggcaaagac tttcctgtga ttgatggtga acacaaatac gagaacattt cttttaaagc
      781 aaacaaaatc gtactcaaag gatttaaaat cattaattct ggtgaagatg aagtgaaaaa
      841 cgttggtgct gtccgccttt acgacagcca ttactccata attgaagaca atatttttga
      901 caataattat tttggaatca ccattcaacg gggctacaaa tgtgttgtca gaaataataa
      961 aattgtatat cgcttcaaac gttcgcaaga aaaaattggt gatggcattc acatctggaa
     1021 cagttctcac cttaaaatcc ttaacaatta tattgaagga actaaggatg gtatttacct
     1081 agaaaaagca tcgcatgtta ttgttgatgg caattattct actcgaaatt tgagatacgg
     1141 ccttcatttc atgttttcaa acgacaacag ctattctaac aatacattca ataacaatta
     1201 tgcaggtgtt gctgtaatgt atagcatgca tgtggatatg tataaaaaca aattcatcaa
     1261 caattgggga gatggtgcct atggcgtttt attgaaagaa atttctttta gtaaaattga
     1321 aggaaacatt tttgataaca acacagctgc tgtttatatg gatggagcta caaaaattga
     1381 tttttttaaa aataagtttg ccaacaacgg atggggcgta aaaatcaatg ccaattgcat
     1441 ggaaaataaa tttgcctaca accattttga aaacaacact tttgatatga gcaccaacgg
     1501 gtctttggta atgaatacct ttaacaagaa ttattgggac aaataccaag gatacgattt
     1561 aaacaaaaac aaaattggcg atattcccta tcacccactc agtttgtatt cagttttatc
     1621 cgataaaaat ccaacgatta tgttgctcta tcgttcgttt attgttgatt tattagatag
     1681 aattgagaaa atgattccaa gttttactcc cgataatttt atcgataaat ttcccttgat
     1741 gaaatcgcca atcaaagatt aatgcgccat gataacaatt aaaaatctca caaaaaaatt
     1801 caataaattc attgctctaa acaatgtaaa tttatccttt agtcaaggtg aagcaattgc
     1861 attaattggt ccaaatggtt gcggaaagac aacgttgatt aagagtattt tggggctgaa
     1921 tgttgtgaaa tcgggtacaa ttgaagtgct caacaacgat atcagtaaca actacaccta
     1981 tagagaaaac attggttaca tgcctcaaat tggtcgctac ccagaaaaca tgactattga
     2041 gcaaaccatc aacatgatta ttgaagcacg caacgttcct aaaaatcagt tggatttaga
     2101 actgttggaa gcctatcaat tggaaagtat ttattctaaa aaaatgagca ccttatctgg
     2161 tggtacaact cagaaagtga gtgctgtact ggcttttttg tttcatccta aaattattat
     2221 tttggacgaa ccaacggcag gattggaccc attggcagca gaaattttaa agaataaaat
     2281 catcaaagaa aaaaacaacg gtaaactcat catcattacc tcacatttgc ttagtgaact
     2341 tgatgatata atcagccaag ttgtttttat taaagaagga acggtaattt tccatcaatc
     2401 tgttgatgat ttgaaaaaaa atacagcaag ttcaaaaata tcggaagcaa ttattaatgt
     2461 attaaaaagc aacaatcatg aatagaatca ttcgttttat cgtatttgat atcatcaaaa
     2521 ataaaattgt acttttttat acgattttat tattcatcat ttcatggtcg gtgcttggaa
     2581 tagaaaccaa ttacaccaaa tccatattaa gcttactcaa cgttgttttg cttgttgtgc
     2641 cgctggttag tgttgtgttt tcaactatct atgtttacaa tagcaatcaa tttatagagt
     2701 tgcttttaag tcagcctgtt ccgcgttcaa aaatttggtt gagtatgttt ttgggcttat
     2761 cttctgccct atcttttgct tttattattg gttgtggact acctatttta atttacagtt
     2821 ctattgaaat aggtttttct ttggtgattt cgggcgtttt tttatccatc atatttaccg
     2881 caatagctat gttagctgcc atttt
//
LOCUS       DAOQVQ010000298         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_40269_length_2904_cov_3.798877, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000298 DAOQVQ010000000
VERSION     DAOQVQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40269_length_2904_cov_3.798877"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKN22_08650"
     CDS             <1..878
                     /locus_tag="PKN22_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42813.1"
                     /translation="YTITFTVNDSYISKSRFDAANSKPVVNSYTRPADQAQPYSEVRY
                     CTNFINANASRLAALGMKASISIDTSKAPSLSGKTIYAEVFKWNDAISITAPTFDNLE
                     SVASAEYNFVNEVDTEDVHFIFDDPLYLEDAQSYLFCLINYGEYFYIGYDRNVDFNAT
                     MQQYNEPMNPLYIMPGQNNQNWFLTGFGSDMTPSIAIHAINQAEAKLNALSSLSGSAY
                     PNPANDVINFNIALDGKATIVVTDVTGKQVASNNVSFVSNTASMNISNLVNGMYIVNV
                     NYENGASAKFNFVKK"
     gene            complement(949..1245)
                     /gene="raiA"
                     /locus_tag="PKN22_08655"
     CDS             complement(949..1245)
                     /gene="raiA"
                     /locus_tag="PKN22_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNS42814.1"
                     /translation="MDIKVNSVNFTPDKKLVSFVNEKVKKLQLFFDNITASEVFLKLD
                     KDQEKENKQAVIKILLPGKELLAKKQCKTFEEATDLACEALSRQVQKHKGKLTD"
     gene            complement(1248..2126)
                     /locus_tag="PKN22_08660"
     CDS             complement(1248..2126)
                     /locus_tag="PKN22_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNS42815.1"
                     /translation="MTPFLFSIEFEQYLQAEKRFSTHTVIAYQHDLMQFFDFTAITTP
                     QDLVEINSKLIRGWMVQLINEGYTNKSVNRKLSAVRAYFRFLKRNGVVAVNPTSGLTG
                     PKIEKRLPQFAKEQELDNQILNNSLSIRDALIVELLYQTGMRLNELIELKTSSLDDCK
                     LKVLGKRNKERIIPISKELVEQIKAYRQDCNQNGFQNSSLILTDKGNKLYPKFVYRKI
                     NAYLASATELQKCSPHVLRHTFATHMLNNGAGIETIKELLGHANLSATQVYTHNSFAQ
                     LTNIYSQAHPRGHKTD"
     gene            complement(2167..2358)
                     /gene="rpsU"
                     /locus_tag="PKN22_08665"
     CDS             complement(2167..2358)
                     /gene="rpsU"
                     /locus_tag="PKN22_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNS42816.1"
                     /translation="MLIVQVKEGESIERALKKYKKKYDKTGVVKQLRNRKEFTKPSVI
                     KRQALIKAVYKQKMQVQEA"
     gene            complement(2437..>2904)
                     /locus_tag="PKN22_08670"
     CDS             complement(2437..>2904)
                     /locus_tag="PKN22_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS42817.1"
                     /translation="IGEVGMGFIQAMQILDGGRISIAALSCGVARGAYEASVKYAKER
                     EQFGKPIATFQAIAFKLADMATEVEAAELLTFQAAYLKNTKQPLTRQGAYAKYYASEV
                     AVRCGNEAVQIMGGYGYTKEYPAEKFLRDAKLLTIGEGTSEIQKLVISREILK"
BASE COUNT          901 a          505 c          439 g         1059 t
ORIGIN      
        1 attatactat tactttcaca gtaaatgata gttatatttc aaaatctaga tttgacgcag
       61 ctaatagcaa acctgttgta aactcatata cacgcccagc agatcaagct caaccatata
      121 gtgaagttag atactgtaca aattttataa atgctaatgc aagtagatta gcagcacttg
      181 gaatgaaagc tagtatttct attgacacat ctaaagcacc aagtttaagt ggcaaaacta
      241 tctatgcaga agtttttaaa tggaacgatg caataagtat tactgcccca acttttgaca
      301 acttagagtc tgttgcaagt gcagaataca actttgtaaa cgaggtggat acagaagatg
      361 ttcattttat ttttgatgat ccattgtatt tagaagacgc tcaaagctat ttattctgtt
      421 taattaatta cggagaatat ttttacattg gatacgatag aaacgttgat tttaacgcta
      481 caatgcaaca atacaacgag cctatgaatc cattatatat tatgcctgga caaaacaatc
      541 aaaactggtt tttaactggt tttggttctg atatgactcc atcaattgct attcatgcaa
      601 ttaatcaagc agaggctaaa ttaaatgcgt tatctagttt aagtggttct gcttatccaa
      661 accctgcaaa tgatgtaatt aactttaaca ttgctttgga cggtaaagca actatcgtgg
      721 taacagacgt tacaggtaaa caagttgctt caaacaacgt tagctttgtg agcaacactg
      781 catcaatgaa catttctaac ttagtgaatg gaatgtatat tgtaaatgta aattatgaaa
      841 acggagcgag tgctaaattc aacttcgtta agaaataatt aggtatttac caataaatga
      901 aaagagcgac caaaagtcgc tctttttgtt ttaaatattt aacgtttttt aatctgttaa
      961 cttcccttta tgtttttgaa cttgtctact caatgcttca caagctaaat cagttgcttc
     1021 ttcaaaagtt ttgcattgtt ttttagctaa taattcttta cctggtaata atattttaat
     1081 gaccgcttgt ttattttctt tttcttggtc tttatccaat tttaagaata cttcacttgc
     1141 tgtaatgtta tcaaaaaaca gttgtagctt cttaaccttt tcatttacaa acgacactaa
     1201 ttttttgtct ggtgtaaaat ttaccgaatt cactttaata tccatggcta atctgttttg
     1261 tgcccacgtg ggtgagcttg tgagtaaata ttagttaatt gtgcaaatga attgtgagtg
     1321 taaacctgcg ttgccgataa atttgcgtga ccaagtagct cttttattgt ttctattcca
     1381 gcaccgttat tgagcatgtg ggtagcaaat gtatgtcgaa gaacgtgtgg gctacatttt
     1441 tgcaattccg ttgcactagc aagataagca tttattttac gataaacaaa ttttggatac
     1501 aatttgttac ccttatctgt taaaataagt gaagaatttt gaaagccatt ttgattgcaa
     1561 tcttgtcgat aagcttttat ttgctcaact agttctttag aaattggtat tattcgttct
     1621 ttattgcgtt taccaagcac tttaagttta caatcgtcta aactacttgt cttaagttca
     1681 attaattcat tgagacgcat acctgtttga tacagcaact caacaattaa tgcatcacga
     1741 atagataatg aattattgag aatttgatta tccagctctt gttcttttgc aaactggggc
     1801 aatctttttt caatttttgg tccagttagc cctgacgttg gatttactgc cactactcca
     1861 tttcttttta aaaaacgaaa ataagcacgc acagccgata attttctatt aacactttta
     1921 ttggtatagc cttcattaat taattgcacc atccagcctc taataagctt tgaattaatt
     1981 tctactaaat cttgtggggt tgttattgca gtaaaatcaa aaaactgcat taaatcgtgc
     2041 tgataggcaa taacagtatg agtagagaaa cgtttctcag cttgtaggta ttgttcaaac
     2101 tctatagaaa ataaaaaagg agtcatatag actccttata cgtatttttt gaaaatatgt
     2161 tatatcttaa gcttcttgaa cttgcatttt ttgcttgtaa acagctttaa ttaatgcttg
     2221 tcttttaatt acagaaggtt ttgtaaattc tttacgatta cgcaattgtt taacaacacc
     2281 tgttttgtcg tattttttct tgtatttttt caaagctctt tcgatgcttt caccttcttt
     2341 tacttgtact attaacatat ttttctattt tttataatct tatcaaaatc cttaatggac
     2401 cgcaaagtta taacattttt ttcaacaaac aactttttat ttcaaaattt ctcttgaaat
     2461 aactaatttt tgtatttcag aggtaccttc accaatagtt aacaacttcg catcacgcaa
     2521 gaatttctct gctggatact cttttgtata accataacca cccataattt gaacagcttc
     2581 attaccacat ctaacagcta cttctgatgc ataatatttt gcataagcac cttggcgtgt
     2641 taatggttgt ttggtgtttt tcaaatatgc tgcttggaaa gttaataatt ctgcagcttc
     2701 aacttctgtt gccatatctg ctaatttgaa tgcaatagct tgaaacgttg caattggttt
     2761 tccaaattgc tctctttctt ttgcatattt aacagatgct tcataagctc cacgtgcaac
     2821 accacaactt aatgccgcaa ttgaaatacg accaccatcc aaaatctgca tcgcttgaat
     2881 aaagcccatt ccaacttcac caat
//
LOCUS       DAOQVQ010000299         2888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_40620_length_2888_cov_4.025415, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000299 DAOQVQ010000000
VERSION     DAOQVQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40620_length_2888_cov_4.025415"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PKN22_08675"
     CDS             complement(<1..431)
                     /locus_tag="PKN22_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42818.1"
                     /translation="MHFKRPIAILFILISTVLFGQDKAMDYSSCEGTLNIFRTQKYNL
                     NFLGNTEPSTTFAEYPALSKITSGNQIWFSFIAPADGEISMSLESSSSNLSLLVFDTL
                     FSYNICNEIKMGLAEIIRMVTPTASKKVGLSKNVNDSYLYP"
     gene            complement(509..>2888)
                     /locus_tag="PKN22_08680"
     CDS             complement(509..>2888)
                     /locus_tag="PKN22_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNS42819.1"
                     /translation="TDLTFNRLKLNDSITLDNFKFITNGNGQNLVSQLVWNPQTENAT
                     DIQWRTKILSSKKFDFVFEPSFFTINKQHWEISKEALVSIDSTSIDVTNFKLSSGEQF
                     ILANGKVSKNDADKLKFELNEVDLNKLSLMVGLNKTLAGKLNGWGYISNPYKNLSYMG
                     DVKVLGLHVNNEEVGDVYAFSQWDKENNKVELSGDLLYRGLPTFQFKGMYDVSKKTDN
                     LDFKLVFDKTNISFVNAFLEPLVIGNIKGFINGNLDVKGSISRPEIEGEVQLDNASAK
                     VVMLGTTYKFNGPIKADKDGFYIDYIPVTDEEGNTGSMTGSIYHNDYKDWNFDLSLDL
                     EEDYYKRDPEKSWVKLPLQKFLAMNTDGKKGELYYGKAYATGTVGIFGYLKNLEIDVN
                     LKAQKGTWVDLSLFRQTELKEDNFIEFVSVDTTQQTPQKKINFSGVTLNLNFDVTPDA
                     QVKMIFNEQTNDAITAFGNGKINMKMDNLGQLSLDGSYSTVEGSKYNFVLGPIKETFY
                     IADGGTITWTGNPYNATLNLDAYYKLKANLGDLSPELLVSGNQEINCFLKLTESLMKP
                     AIQFDIKAPKAPDTDKSILAQLTSEPDELNRQFFSLLLWKKFQPSKGSARANGGAALD
                     LATNQINSLLSQVSQDYKLNVDMNSDNQGGSEYAFGIEKGFLDDKLVLSGSFGARNST
                     KGNQTQSSVIGDIELEYKLNKEGTFRINVFNESNDNRNLQVSNDRGMFKQGVGIYYKE
                     SFNNSKDFKLLQKFLDIFRKKQNKRYPVRRKKIQTPVPKEDEAQPQIQQDKN"
BASE COUNT          872 a          547 c          444 g         1025 t
ORIGIN      
        1 attgggtata aataactatc gttaacgttt ttgctaagcc ccactttttt actggccgtt
       61 ggtgttacca tacggatgat ttctgccaac cccattttaa tttcattgca aatattgtag
      121 gaaaacaagg tatcaaaaac caaaagtgat aaattagaac tcgatgattc taggctcatt
      181 gaaatttcac catctgctgg ggcgataaac gaaaaccaaa tttgatttcc cgacgtaatt
      241 ttggataacg ctggatattc tgcaaaagtt gtagatggtt ctgtgtttcc tagaaaattc
      301 aggttgtatt tctgtgttct aaaaatgttt aaagtacctt cacacgatga gtaatccatt
      361 gctttgtctt gaccgaacaa aacagtagaa attagtataa acaatatcgc aataggtctt
      421 ttaaagtgca taattctagg actaaattaa tgattttaat tgaaatacat tgttttttca
      481 atgaataacc aataaatatg cctaaaattt agtttttatc ttgctgaatt tgcggttgtg
      541 cctcatcttc cttaggaaca ggcgtttgta tttttttacg acgaacggga tagcgcttgt
      601 tttgtttttt tctaaaaata tccaagaact tttgcaagag tttaaaatct tttgaattgt
      661 taaaactttc tttataataa atacctacac cttgtttaaa catccctcta tcgttgctca
      721 cttgcaagtt gcgattgtcg tttgattcat taaaaacatt aattctaaac gttccttctt
      781 tgttcaattt gtattccaat tcaatatcac caataactga tgattgtgtt tggttacctt
      841 tggttgaatt tctagctcca aacgaacccg acaataccaa tttatcgtct aaaaatcctt
      901 tttctattcc aaaagcatat tcacttccac cttgattgtc tgagttcatg tccacgttta
      961 acttataatc ttgtgaaact tgtgacaaca aggagttgat ttgattggtt gctaaatcca
     1021 atgctgcacc tccatttgct cttgcactac ccttggatgg ttggaatttt ttccacaata
     1081 acaacgagaa aaactgacgg ttcaactcat ctggttctga ggttaattgg gcaagaatgg
     1141 atttatcggt atcgggtgct tttggtgctt taatatcaaa ctgaatggct ggtttcatca
     1201 acgattcagt taatttcaaa aaacaattaa tttcttggtt accactaact aataattctg
     1261 gcgataaatc acccaagttg gctttcaatt tgtaataagc atctaaattg agcgttgcat
     1321 tgtatggatt tccagtccac gtaatggttc caccatctgc aatgtaaaac gtttctttaa
     1381 ttggaccgag cacaaaatta tatttacttc cttcaacggt tgaataagaa ccatccaaag
     1441 aaagttgccc taaattgtcc attttcatat tgatttttcc gtttccaaaa gctgtaatag
     1501 catcgttggt ttgttcgtta aaaatcattt ttacttgtgc atctggcgtt acatcaaaat
     1561 ttaagttgag cgttacacct gagaagttaa ttttcttttg aggtgtttgt tgagtggtat
     1621 caactgaaac aaattcaata aaattatctt ctttcaattc ggtttgacga aacaacgata
     1681 aatccaccca agtgcctttt tgtgctttta agttgacatc aatttctaag tttttcaaat
     1741 agccaaaaat tccaacagta ccagttgcat aggcttttcc ataatacaac tccccttttt
     1801 ttccatcggt attcatggcc aagaattttt gcaaaggcaa tttcacccaa cttttttctg
     1861 gatcgcgttt atagtagtct tcttccaaat ccaacgacaa atcaaagttc caatctttgt
     1921 aatcattgtg atagatggat ccggtcattg agcccgtgtt tccttcttca tcggttactg
     1981 gaatatagtc aatataaaaa ccatctttat ccgcttttat tggtccgttg aatttgtagg
     2041 ttgttcccaa cataaccact tttgcagaag cattgtccaa ttgtacttca ccttcaattt
     2101 ctggtcgcga aatagaacct tttacatcca aatttccgtt gataaaccct ttgatattac
     2161 caattaccag tggttctaaa aatgcattta caaacgaaat gttggttttg tcaaacacta
     2221 atttaaaatc taaattatcc gtttttttac ttacatcata catgccttta aactgaaaag
     2281 ttggcaagcc acgatacaat aaatcacccg ataattctac tttattattt tctttatccc
     2341 attgagaaaa cgcatataca tcccccactt cttcattatt aacatgcaat cccaacactt
     2401 tcacgtcgcc catatacgac aagtttttat acggattgga aatgtagccc caaccgttta
     2461 gttttccagc caatgttttg ttgagaccca ccattaaact caatttattc aaatccacct
     2521 cattgagctc gaacttcaat ttatcggcat catttttaga aactttaccg ttggcaagaa
     2581 taaattgttc accactcgat aatttaaaat tggttacatc aattgacgtt gaatcgatgc
     2641 tcaccaaagc ttcttttgaa atttcccagt gttgtttgtt gatggtgaaa aaagaaggtt
     2701 caaacacaaa atcaaacttt ttggatgaaa gaatttttgt gcgccattga atatcggttg
     2761 cattttcggt ttgcggattc cataccaatt gagaaactaa attttgtccg ttaccattgg
     2821 tgataaactt aaagttatcc aaggtaatag aatcatttaa tttaagtcta ttaaaggtta
     2881 aatcggta
//
LOCUS       DAOQVQ010000300         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_40892_length_2874_cov_3.207166, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000300 DAOQVQ010000000
VERSION     DAOQVQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40892_length_2874_cov_3.207166"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PKN22_08685"
     CDS             <1..959
                     /locus_tag="PKN22_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42820.1"
                     /translation="QLWAQSDSLPYTSYRYRPIIFADIGFTAAPLDFNYPFSQDVKQL
                     QFRHNNKIMLGIGASYSWFAFRLGFAVIGNFIPVKHYGKSNYYDLGFRFSIKRFFSEW
                     DFRAYTNYALLNTYKWDSTFSKELPNDTRQKIDVFNVSAKMWYINNKNYKMDAFTGNR
                     GVFNKPTFTWYLGGRLDFYGIANKVGPIIPPMLQDSTYSKTRATVFSAVELGVLPGVG
                     YVTRYKTFQFGIMAGLGPMLQFKSYTIDGTPTVLGAVTLRYDFKMAFGYNVPRCFVMF
                     LFDVDTKSIGYDKLKYNQTFYSMRMQFGYRFNEKIPKKKRKK"
     gene            1284..2312
                     /locus_tag="PKN22_08690"
     CDS             1284..2312
                     /locus_tag="PKN22_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNS42821.1"
                     /translation="MTEIYKTLDDRKEHLPFYKVPSHYLNGFFTVIFGGSTGIEVSTV
                     VATAALGNNMYKRNISAHIYKREIVCAGVAAGVAILFSSPLAGWLFAIEVIARKLSKT
                     IVISCTTAVAVASIYIFFIDDTRILPYEITHWTWMAIPFFIVLSCMGGALSAYFTLLV
                     TRMKPLFSKLPNDYYRVVLGSLIVGVLLFIFPQLYGDSYKGLHDVIGDSMKIGGISLI
                     ALFLLALLKPLAAAVTLGAGGDGGVFAPSIVAGAFLGILFAQLCNTYFDTNFIMLNFA
                     LVGGASMLAASINAPMTTIVLISNIVPNGYQLALPLTLCCFVAVFIAKKIIPYTVYTY
                     HLFEKEKS"
     gene            complement(2322..>2874)
                     /locus_tag="PKN22_08695"
     CDS             complement(2322..>2874)
                     /locus_tag="PKN22_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNS42822.1"
                     /translation="ADSLIVDPHKGLFLPYGTGAVLIKNKAATLHSNYYEANYMQDAT
                     NDALVKSAANISPELTKHFRGLRVWLPLQLHGIAPFVACAEEKLVLVQYFRQRLVDLG
                     FEVGPTPDLSVSYFWFPFKENRDAKNKQLMDAIHQDGEVFLSSSIIKGQFVIRMAILS
                     FRTKKETIDRAVAMIERCLQKVK"
BASE COUNT          833 a          551 c          520 g          970 t
ORIGIN      
        1 atcaactttg ggcgcaaagt gattcacttc cctacacttc atatcgttat cgtccaataa
       61 tttttgcaga cattggattt acagcagctc cgttagattt caattatcct tttagtcaag
      121 atgttaaaca acttcaattt cgtcacaaca acaaaattat gttgggtatt ggagcttcat
      181 acagttggtt tgcttttcgc ttaggttttg ctgtgattgg taattttata ccggttaaac
      241 actacggaaa atcgaattat tacgatttag gtttccgatt ttctatcaaa cgcttttttt
      301 cggaatggga ttttagagcc tataccaatt atgcgctgct gaatacgtat aaatgggatt
      361 caactttttc caaagaattg cccaacgata cccgccaaaa aattgacgtt ttcaatgttt
      421 ctgcaaaaat gtggtacatt aacaataaaa actacaaaat ggatgctttt acaggcaaca
      481 gaggtgtatt caataaacca acttttacgt ggtatttggg tggacgcttg gatttttatg
      541 gaattgctaa taaagttgga ccaataattc cacccatgtt gcaagattct acctattcca
      601 aaacacgagc aactgttttt agtgctgttg agttgggagt tcttccaggt gttggttatg
      661 tgacgcgcta caaaacattt caatttggaa taatggctgg tttgggtccc atgttgcaat
      721 tcaaatccta tacaattgat ggcacaccaa ccgttttggg ggctgtaaca ctgcgctatg
      781 attttaaaat ggcatttggc tacaatgttc cacgctgttt tgtgatgttt ctgtttgatg
      841 ttgataccaa atccattgga tacgataagt taaaatacaa ccaaactttt tattcaatgc
      901 gcatgcaatt tggttatcgt ttcaacgaaa aaatcccgaa gaagaaacgt aagaaataat
      961 tcgatactac aatcaaaaaa ataaagaatc attttctttt tagagttcta caccttactt
     1021 ttgcatcgag tttagtctat gaaccaacgt aagaaaatag tatatcacaa ttatggcaag
     1081 ctaattttaa atgctgccct tgttggttta ctaagttctc tacttgctgt atctctaaaa
     1141 tttattaccg atttttttga acaccaactc attcatactg cattttctac ctctacttat
     1201 ttgtttttat tttaccactc attggtattt tcatcatcta ttttttacgt gttagttttt
     1261 ttaaaaacaa aaagaataaa gggattaccg agatttacaa aacgttggat gatagaaaag
     1321 aacatttgcc tttttataaa gttccctcgc attatttgaa tggttttttt accgtgattt
     1381 ttggcggttc aacgggaatt gaagtttcta cagttgttgc aacagcagct ttgggaaaca
     1441 atatgtataa acgcaatatc tcggcacata tatacaaacg cgagattgtt tgtgcaggag
     1501 ttgcggcagg cgttgctata ttattttcaa gtccgctggc aggttggttg tttgccattg
     1561 aagtcattgc tagaaaacta agcaaaacaa tagtcatcag ttgtacaacc gcagtagccg
     1621 ttgcaagtat ctacattttc tttattgacg atacccgcat tctaccttat gaaataaccc
     1681 attggacctg gatggcaatt ccatttttca ttgttctaag ttgcatgggc ggagcacttt
     1741 ctgcatattt cacactattg gtaactcgca tgaaaccatt gttttctaaa cttcctaacg
     1801 attactaccg cgttgtgcta ggctcgctaa tagttggagt attacttttt atctttccac
     1861 aattgtatgg agatagctac aaaggacttc atgacgtaat tggtgatagt atgaaaatcg
     1921 gtggtatttc attaattgca ttgtttttat tagccctact taaaccactt gcagcagctg
     1981 ttactcttgg tgctggtggt gatggcggtg tgtttgctcc aagcattgtg gcaggtgctt
     2041 ttttaggaat tctgtttgcg caattgtgca acacttactt cgacacaaat tttataatgc
     2101 tcaattttgc acttgttggt ggagcgtcca tgttagcagc atccatcaat gcgcccatga
     2161 ctaccatcgt tttgattagc aacatcgttc caaatggtta ccaattagca ctaccgctta
     2221 cactttgttg ttttgtagcg gtttttattg ctaaaaaaat cattccctac acggtttaca
     2281 cctaccacct ttttgaaaaa gagaaaagct aaagtttaca actattttac tttttgcaaa
     2341 caccgttcaa tcatcgctac cgccctatca atggtttctt ttttagtgcg aaatgataga
     2401 attgccatac gaatcacaaa ttgcccttta atgattgaag aacttaaaaa cacctcgcca
     2461 tcttggtgaa tggcatccat caattgtttg ttttttgcat ctctattttc tttaaaagga
     2521 aaccaaaaat aactcaccga taaatcaggg gtgggaccaa cttcaaaacc taaatctacc
     2581 aatcgttggc ggaaatattg caccaacacc aatttttctt cggcacacgc aacaaatggc
     2641 gctattccat gcaattgcaa aggcaaccat acacgcaaac ctctaaagtg tttggtgagt
     2701 tcgggcgaaa tattggctgc actttttacc aaagcatcgt tggtggcatc ttgcatatag
     2761 ttggcttcgt aataattgga atgcaaggtg gccgctttat ttttaattaa aacagcaccc
     2821 gttccgtaag gcaaaaacaa acctttgtga gggtcaacaa ttagcgaatc ggct
//
LOCUS       DAOQVQ010000301         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_41148_length_2862_cov_4.126470, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000301 DAOQVQ010000000
VERSION     DAOQVQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41148_length_2862_cov_4.126470"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKN22_08700"
     CDS             complement(<1..213)
                     /locus_tag="PKN22_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS42823.1"
                     /translation="MPKPNSQPVSCQTCEVRHLSLFKSLCAEGVNELNDIKDCIIYKK
                     NEPVFVEGAYPKGIFCIKAGKIKVFTI"
     gene            complement(278..1030)
                     /locus_tag="PKN22_08705"
     CDS             complement(278..1030)
                     /locus_tag="PKN22_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS42824.1"
                     /translation="MFAASISALILGLGASLHCLGMCGPLVMAVPISSKTWQGKAVGI
                     TQYHLGKTFTYALLGLVVGVIGISLQTLKWMQILSIITGVLIILFAWKKFIKLPASNK
                     IQQFLTRFSGKSLNKLFKSTIPFKPFFFGMINGLLPCGLIYIALLNSLLAGDPFSSMV
                     AMAFFGVGTIPILTLAKFASLKFNWQASKITPVLVTVVGLLIIVRGMNLGIPYISPKI
                     NTSIIASSDKDVKQTVSMDCCHKSDHCNTTKK"
     gene            complement(1034..1453)
                     /locus_tag="PKN22_08710"
     CDS             complement(1034..1453)
                     /locus_tag="PKN22_08710"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNS42825.1"
                     /translation="MNWGKGLTIAMALFIGFIITLTSIMMSKSTELEDEDYYAREVNY
                     EQEIQSYKNAATLGKATVNLVDDEIQIVFPEDLALSDGKVVLSRPNNQKLDQEFYFEE
                     GNSFVIPKSKLVAGKYNIEILYKINNQSCIQKEEITI"
     gene            complement(1466..>2862)
                     /gene="ccoG"
                     /locus_tag="PKN22_08715"
     CDS             complement(1466..>2862)
                     /gene="ccoG"
                     /locus_tag="PKN22_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNS42826.1"
                     /translation="DEEEFRNSIATVDKDGKRVWVYPKKPWGAYFTKRKIVSYSLLLI
                     LIGLPFIKVGGEPFLMLNIVERKFIIFGNIFWPQDFYIFALSMIIGVVFVIFFTIIFG
                     RLFCGWFCPQTIFMEFVFRQIEYWIEGDFKHQIKLNQQPWNREKILKKTAKFTLFYLI
                     AFFIANIFLSYIIGIDALWNIITSPVSEHVGGFIAIILFSFVFFWVFARLREQVCTTI
                     CPYGRLQGVLLDRNTMVIAYDYVRGEGEKGRAKFKKNEDRQAAGKGDCIDCKQCVYVC
                     PTGIDIRNGTQLECVNCTACIDACDHMMDSVGLERGLIGFLSEENIKNREKFKFTPRV
                     KAYSALLTALIITLVIMLVTRSDFQTNIARQRGTTFQITEDGKVSNIFEIYIANKTRK
                     GYDIQLKLEGNHGEIETAVKKLYLKPEKELKERFVVKIPYKEVSDKEVIYINVYGNGK
                     KIQHVKTKFIGPSF"
BASE COUNT          933 a          553 c          421 g          955 t
ORIGIN      
        1 aatagtaaat actttaattt ttccagcttt gatacagaaa atgcctttag gataagctcc
       61 ttcaacaaaa acaggttcgt ttttcttgta aataatacaa tcttttatgt cattcaattc
      121 atttactccc tccgcacaca gcgatttgaa taatgaaaga tgacgcacct cgcaagtttg
      181 acaacttacg ggttgactgt ttggtttggg cattgcactt tttttatgca aatatataga
      241 taaacttatt gataaatgaa atataaaaaa gtagttttta ttttttggta gtattgcaat
      301 gatcggattt gtgacagcaa tccattgata ctgtttgttt tacatcttta tctgatgaag
      361 caataatcga tgtatttatt tttggactta tgtacggtat ccctaagttc atgcctctta
      421 caatgattaa aagtccaaca acagttacca aaacgggtgt tattttactt gcctgccaat
      481 taaatttaag tgatgcaaat tttgcaagtg ttaaaatagg aatggtcccc acgccaaaaa
      541 atgccattgc caccatagaa gaaaacgggt cgccagccaa caagctgttt aaaagtgcaa
      601 tatagattag cccacatgga agcaacccgt taatcatccc aaagaaaaaa ggtttaaatg
      661 gaatagttga tttgaataat ttattcaatg atttaccaga aaatcgtgtt aaaaattgtt
      721 gaattttatt ggatgcagga agttttataa attttttcca agcaaaaaga ataattaaaa
      781 cacctgtgat gatggataaa atttgcatcc attttaaggt ttgcaaacta ataccaatca
      841 ctccaactac caatcccagc aatgcataag taaatgtttt tcccaaatgg tattgagtaa
      901 ttccaaccgc cttgccctgc catgttttag aagaaatagg cacagccatc accaacggcc
      961 cgcacattcc caagcagtgt aaagaagccc caaggcccaa aattaaagca cttattgacg
     1021 ctgcaaacat tttttaaatg gttatctctt ctttttgaat acaagattgg ttgtttattt
     1081 tatacaaaat ttcaatgttg tattttcctg ccaccaattt ggatttagga attacaaatg
     1141 agtttccttc ttcaaaataa aactcttggt ccaacttttg gttgtttggt cgactcaaca
     1201 ctactttccc atccgataaa gctagatcct caggaaatac aatttgtatt tcatcgtcca
     1261 ccaaattaac ggttgcttta cccagagttg cggcattttt ataagattga atttcttgct
     1321 cgtaatttac ttcacgagca tagtaatctt cgtcttctag ttcggtactt ttgctcatca
     1381 taatagatgt aagggtgata ataaacccaa taaaaagtgc catcgctatt gttaatcctt
     1441 tcccccaatt catttcaatt attttttaaa aacttggtcc aataaatttc gtttttacgt
     1501 gttgaatttt ctttccattg ccatatacgt tgatgtaaat cacctctttg tctgatactt
     1561 ctttatatgg aattttcacc acaaaacgtt ctttcaattc tttttctggt tttagataca
     1621 actttttaac agctgtttca atttcaccat gattcccttc taattttaat tgaatatcat
     1681 accccttacg tgttttgttg gcaatgtaaa tttcaaaaat attgctcact ttaccatctt
     1741 cggtaatttg gaaagtggta cctctttggc gcgcaatatt ggtttgaaaa tcactacgag
     1801 ttaccaacat tattacaagc gtaataatta aagccgttaa caaggccgaa taagctttta
     1861 cacgtggtgt aaatttgaat ttttctcggt ttttaatgtt ttcttcagac aagaaaccaa
     1921 tcaatcctct ttcaagtcca acactgtcca tcatgtggtc gcaagcatca atacaagccg
     1981 tacagttcac acattctaat tgcgtaccat tacgtatatc aataccagtt ggacaaacgt
     2041 acacacattg tttacaatca atacaatccc ctttccctgc tgcctggcga tcttcattct
     2101 ttttgaattt agctctacct ttttcacctt ctccacgcac ataatcgtaa gcaattacca
     2161 ttgtgtttct atcgagtaat actccttgca agcgaccata aggacaaatt gtagtacaca
     2221 cttgttctct tagtcgggca aatacccaaa aaaacacaaa tgaaaaaaga ataattgcaa
     2281 taaaaccacc tacgtgttca gaaaccggtg atgtgatgat gttccaaagt gcatcaatac
     2341 caataatata tgaaagaaag atattggcaa taaaaaatgc aataagataa aacaacgtga
     2401 actttgctgt tttcttaaga attttctcac ggttccaagg ttgttgattt aattttattt
     2461 gatgtttaaa atcgccttca atccagtatt caatttggcg gaatacgaat tccataaaaa
     2521 ttgtttgtgg acaaaaccac ccacaaaaca aacgaccaaa aataatggta aagaaaatga
     2581 caaacactac tccaattatc atggataagg caaagatgta aaaatcttgt ggccaaaaaa
     2641 tgtttccaaa aatgataaat tttctttcaa cgatgtttaa cattaaaaaa ggttcgccac
     2701 ctaccttgat aaatggcaaa cctattaata ttaaaagtaa agaataactt acaatttttc
     2761 gtttcgtaaa atatgcgccc cacggttttt taggatacac ccagactctc tttccgtctt
     2821 tatcaacagt tgcaatactg tttctaaatt cctcttcgtc tt
//
LOCUS       DAOQVQ010000302         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_41178_length_2861_cov_3.602994, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000302 DAOQVQ010000000
VERSION     DAOQVQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41178_length_2861_cov_3.602994"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1532)
                     /locus_tag="PKN22_08720"
     CDS             complement(180..1532)
                     /locus_tag="PKN22_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNS42827.1"
                     /translation="MKHTIYSTLFIALFSVSPLFAQRTITLQDALNYALENSEVMKQA
                     RLDVENGKAKVSESMASAMPQINLNSSITGNPIVQQFVLPKAMMGGSPDEFIAIRAGQ
                     NWSAMTQVTLSQQIYNQQVFAGLKAAKGSASFYELSAQLSEENVLQQVATNYYQLVVT
                     RAKMGVLDANIERVTQLEEILTSQLENGLVKKIDLDRVKVNKTNLIAQKIELENAVTQ
                     MANLLKYYMGMPISEQITVPETALSEMENFGGAISKDQPLNTENLLSMKVMNKQAELL
                     SLQTKVHKAGYIPSLYLSGHYTYNSQSNKFNMYSKKALSYDMSSISLNLTVPLFDGLA
                     RHSRVKSSEIQLKQLREEMTKTTNALTMAYENAKIQIDNSIRTIEVQEANKRMAKEVF
                     DVTQSNYQLGLASLTDLINAETELRSAENSYTEALLQYKVAELELIKSKGEISSLLNK
                     "
     gene            1711..2532
                     /gene="lgt"
                     /locus_tag="PKN22_08725"
     CDS             1711..2532
                     /gene="lgt"
                     /locus_tag="PKN22_08725"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNS42828.1"
                     /translation="MLFIHWDVTPEIFSGLKTPNYYGLLFVTGLILGYYTIRKMFRKE
                     GIADEKLDKLVLYVVIATIVGARLGHVFFYDWSYYSQHPGDIIKVWEGGLASHGGAIA
                     LVIALILYNKLVLKKDYMWIFDRISAPIAIAGTFIRLGNLMNSEIVGRPSDVPWAFEF
                     VHYINETTGQFDPTPRHPAQLYEAIMYLSIFGILMFLFWKKEAWKQPGLTFGVFLILL
                     FGGRFLVEFFKEGQTAYDNTLTINTGQQLSIPFVIAGIIILIWSYKNKSKNAPTA"
     gene            2545..>2861
                     /locus_tag="PKN22_08730"
     CDS             2545..>2861
                     /locus_tag="PKN22_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNS42829.1"
                     /translation="MANKTIDRIKLANFGNLELLAKQVVEGFITGLHKSPFHGFSVEF
                     AEHLLYNKGESTRHIDWKLYGRTEKLFTKKYEEETNLRCQIVIDCSGSMFYPKDVDFT
                     KYE"
BASE COUNT          855 a          497 c          470 g         1039 t
ORIGIN      
        1 ttatagcttc ttttttaact gtaaatactt tcacgggaat ttttccattt ccagtactaa
       61 ctacatcaac ttttgattga ttttcttttt tattgcttgc taatttcatt gcaattccaa
      121 cacctaaaac tgcaattact aatatgtata tgatggcttt tttcattttt gaaaactttt
      181 tatttattta ataacgaact gatttctcct tttgatttaa ttaactccaa ctctgcaact
      241 ttgtattgca ataatgcttc tgtatagcta ttttctgctg atcttaattc tgtttcagca
      301 ttaattaaat cggtcaaact agccaaacct aattggtaat tgctttgcgt cacatcaaaa
      361 acttctttgg ccattctttt gttggcttct tgtacttcaa tggtgcgaat gctattatca
      421 atctggattt tcgcattttc atacgccatt gtcaatgcgt ttgttgtttt tgtcatttct
      481 tcacgcaatt gtttcaattg aatctcagaa gacttaactc ttgagtgtct tgccaatcca
      541 tcaaacagag gaactgtaag gtttaaagaa atagaagaca tatcataact caatgctttt
      601 ttggaataca tgttaaattt gttgctttgt gaattatagg tgtaatgtcc tgataaatac
      661 aagcttggga tgtaacctgc tttatgcact ttcgtttgca atgacaacaa ttctgcttgt
      721 ttattcatca ccttcattga aagtaaattt tctgtattca atggttgatc ttttgaaatg
      781 gctcctccaa aattctccat ttcagataat gccgtttcag gaaccgtaat ttgttcacta
      841 attggcatcc ccatgtaata tttcaacaaa tttgccattt gtgtaaccgc attttccaac
      901 tcgatttttt gagcaatcaa attagtttta ttgactttca cacggtccaa atcaattttt
      961 tttaccaatc cattttccaa ttgtgacgtc aaaatctctt ccaattgcgt aacgcgctca
     1021 atgttagcat ctaaaactcc catttttgct cttgttacaa ccaattgata gtaatttgta
     1081 gccacttgtt gcaacacatt ttcttcactc aattgtgctg atagctcata aaaactagcg
     1141 ctaccttttg ccgcttttaa gcctgcaaac acttgttggt tataaatttg ttgactaagc
     1201 gtaacttgtg tcattgccga ccaattttgt cctgcacgaa ttgcgataaa ttcatctgga
     1261 ctccctccca tcatcgcttt tggcaataca aattgttgca caattgggtt acctgtgatt
     1321 gaagaattca gattaatttg tggcatagca gaagccattg attcggaaac tttcgctttt
     1381 ccattctcga catccaatcg agcttgtttc attacctcgc tgttttccaa tgcataattg
     1441 agtgcatctt gcaacgtaat agtgcgctga gcaaacaaag gtgaaactga aaacagagca
     1501 atgaataatg tactgtaaat tgtgtgtttc atttttccta ataaaaagtg gtgcaaattt
     1561 gatgataatt aatcaattaa taagatagtt tttagttgaa acggacaaaa aaaatgttaa
     1621 acctattatt actatgttca atcgataaaa aaaatggagt tcatcaacga atgtcgtgtt
     1681 tgttttatat ttttacataa aaaataaatt atgttgttta tacattggga tgtaactcct
     1741 gaaatttttt ctggtctaaa aacaccaaat tattatggac ttttatttgt aaccggttta
     1801 atacttggtt attacaccat tcgtaaaatg ttccgaaaag aaggtatagc tgatgaaaaa
     1861 ctggataaat tagtacttta cgtagtaatt gcgaccattg ttggtgctcg tttaggccac
     1921 gtattctttt atgattggtc gtattacagc caacatcctg gcgatattat taaagtttgg
     1981 gaaggcggtt tggcaagtca cggtggtgca attgcactgg tcatcgctct tattttatac
     2041 aacaaattag ttttgaaaaa agattacatg tggatctttg accgtatttc tgctcctatt
     2101 gccattgcag gaacatttat tcgtttagga aatttaatga acagtgaaat tgtgggtcga
     2161 ccatcagatg tgccttgggc ttttgagttt gtacattaca tcaatgaaac aacggggcag
     2221 tttgacccta ctccacgtca tccagcacaa ttgtatgaag ccatcatgta tctttcaatt
     2281 tttggtattt taatgttttt attttggaaa aaagaagctt ggaaacagcc aggtttaact
     2341 tttggcgtat ttctaattct tttgtttggt ggaagatttt tggttgaatt cttcaaagaa
     2401 ggacaaaccg cttatgacaa tacgttaaca attaatacgg gccaacaatt atctattcca
     2461 tttgttattg ctggtattat cattttaatt tggtcgtata aaaacaagtc caaaaacgct
     2521 ccaactgctt aaattgtaga taaaatggca aataaaacca tcgatcggat aaaacttgca
     2581 aattttggca acttagaatt gttggcaaaa caagtggtag aaggttttat tacaggactt
     2641 cacaaaagcc cgtttcatgg tttttctgtt gagtttgccg aacatttatt atacaacaaa
     2701 ggagagtcaa cccgtcatat tgattggaag ttatatggca gaacagaaaa attatttacc
     2761 aaaaaatacg aagaagaaac caatttgcgc tgtcaaattg taattgactg ttcgggttcc
     2821 atgttttacc ctaaagatgt agattttaca aaatatgaat a
//
LOCUS       DAOQVQ010000303         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_41514_length_2847_cov_3.509670, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000303 DAOQVQ010000000
VERSION     DAOQVQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41514_length_2847_cov_3.509670"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PKN22_08735"
     CDS             complement(<1..703)
                     /locus_tag="PKN22_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNS42830.1"
                     /translation="MLLKRVVVTGMGALTPIGNTLDEYWKALLAGKSGAAPIQHFDAT
                     LFKTHFACELKNFNIEDHIERKEARKIDPFAQYAMVSATEAVADSAILDSNPNLDRIG
                     VIWGSGIGGLKTFQDEAQAFFEGDGTPRFNPFFIPKMIADIAAGHISIKYGFRGPNYV
                     TVSACASSTNAIIDAFNMIRLGKADAIVTGGSEACVNEMGMGGFNALKALSTRNDSPE
                     TASRPFDVDRDGFVLG"
     gene            complement(746..979)
                     /locus_tag="PKN22_08740"
     CDS             complement(746..979)
                     /locus_tag="PKN22_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNS42831.1"
                     /translation="MSDIKTRVVAIIVDKLGVEESEVTTSASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAENIQTVGDAISYIEKNA"
     gene            complement(1182..2156)
                     /locus_tag="PKN22_08745"
     CDS             complement(1182..2156)
                     /locus_tag="PKN22_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNS42832.1"
                     /translation="MQNIQTSIFSISNDEQFNELALAVFHYQVENCAVYRQFVEQFPN
                     KQHPQHYTEIPFLPISFFKSHRIAKEHIKEQVVFKSSGTTGQTRSQHFVEDLALYQQS
                     FTKAYQQLIGNPEEQVILALLPNYIEQGNSSLVYMVDQLIQQTKNELSGFLLNQPEKI
                     VERYQAALQQNKKIVLFGVSYALLDLAEQNIHLQKATIIETGGMKGRRKELSKEELHQ
                     ALIKGLKVEYISSEYGMTELLSQAYSDKNGLFQHPAWMKILIRDVNDPFSLVQEGKSG
                     GINVIDLANINSCSFIATEDLGKMEGHLFKVLGRIDLSDIRGCNLLVQ"
     gene            2312..>2847
                     /locus_tag="PKN22_08750"
     CDS             2312..>2847
                     /locus_tag="PKN22_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42833.1"
                     /translation="MFRGFINALIAFFCGGTASAQSPQTPEINPMNVLFIGNSFTHMN
                     SMPVMFERLAISKNIKINVLMSAKSNHTFEMHSKRADLYEDIHKAKWDYVVLQGFSRE
                     FSYGQEHIDTAVIPYFQQIVDTLYQNNPCVKLLLYMTWGYENGYAYMNEIGTYEEMSA
                     AIESGYRYIAGKYNLAIVP"
BASE COUNT          905 a          522 c          477 g          943 t
ORIGIN      
        1 cgcccaatac aaaaccatct ctatccacat caaatggacg agaagctgtt tcaggagaat
       61 catttcttgt agataatgct ttaagtgcgt tgaaaccacc cattcccatt tcgttcacac
      121 acgcttcaga accacctgtc acgatggcat ccgctttgcc taaacgaatc atattgaacg
      181 catcaataat ggcgttggta gatgatgcac atgcagaaac tgtaacataa ttaggacctc
      241 taaacccgta tttgatagaa atatgaccag cagcaatatc tgcaatcatt ttagggataa
      301 agaacgggtt gaaacgtggt gtaccatcgc cttcaaagaa agcttgagct tcatcttgaa
      361 atgttttcaa accaccaatt ccagaacccc aaattacccc aattctatcc aaatttggat
      421 tactgtctaa gatcgcagaa tctgccacag cttcagttgc tgaaaccatg gcatattgtg
      481 caaatggatc tatttttctt gcctctttgc gctcgatgtg atcttcaata ttgaagtttt
      541 ttaactcaca cgcaaaatga gttttaaaca atgttgcatc aaagtgctga attggtgcag
      601 ctccgctttt accagctagc aatgctttcc aatattcatc taaagtatta ccaattggag
      661 ttagcgcccc cattccagtt acaacaactc tttttaacaa cataaatcta gtttcaagaa
      721 aaattaatac ttgttatctt attaattatg cgtttttctc aatgtaagaa atagcatcac
      781 caacagtttg gatgttttct gcttgatcat ctggaattgc aatgttgaat tctttttcga
      841 attccataat caattctact gtgtctaatg aatcagcacc aagatcattt gtgaagcttg
      901 cacttgttgt tacttcgctt tcttcaactc ctaatttgtc aacgataatc gctactactc
      961 ttgttttaat atcagacatt tttctaattt ttaaaggtta atcggcaaca aagaaaaaaa
     1021 caaacattca attatcaaaa ttatttgcat agttttctat tgaacgactt ttatttgttg
     1081 tgctaatata ttgaaaataa atataataaa tgattttttg ttgaaaaata tttggcaagt
     1141 attaacaact cgtcaaaaaa aggataaaaa tctacatttt tttattgaac tagtaggttg
     1201 caacccctga tatctgaaag atcaattcta cccaaaactt taaataaatg gccttccatt
     1261 ttccctaaat cttcagtagc aataaaacta caactattaa tattggccaa atctatcacg
     1321 ttgattccac cacttttgcc ttcttgcacc aatgaaaacg gatcgttgac atcgcgtatg
     1381 aggattttca tccatgctgg gtgctgaaac aaaccatttt tatcgctgta ggcttgcgac
     1441 aaaagttcgg tcatgccgta ttcggaagaa atgtattcta ccttcaatcc ttttattaat
     1501 gcttggtgca gttcttcttt tgaaagttct tttcttcgcc ccttcattcc tcccgtttca
     1561 ataattgttg ctttttgaag atggatgttt tgttctgcta aatccaacaa agcatacgaa
     1621 acaccaaaga gaacaatctt tttattttgt tgcaaagcgg cttggtaacg ttctacaatc
     1681 ttttcgggtt ggtttaataa aaaccccgac aactcatttt ttgtttgttg aattagttga
     1741 tccaccatat acaccaaact cgaatttcct tgttctatat aattaggtaa caatgctaaa
     1801 attacttgct cctctggatt accaatgagt tgttgatatg cttttgtaaa ggattgttga
     1861 tacaacgcaa gatcttctac aaaatgctgg cttcttgttt gccccgttgt tccgctactt
     1921 ttaaaaacta cttgttcttt gatgtgttct ttagcaatgc ggtgactctt aaaaaaagaa
     1981 atgggcaaaa aaggaatttc tgtataatgt tgcgggtgtt gtttgttggg aaattgctct
     2041 acaaactgac ggtaaacagc acaattttct acctgataat gaaaaacagc aagtgcaagt
     2101 tcgttaaatt gttcatcgtt ggagatagaa aatatagatg tttggatatt ttgcacagta
     2161 aaacaggtgt ataaattatt gtcaattaca aaataaataa aaaaatcaaa ataaatatta
     2221 atcaataaaa tgtaatttcg cattttacaa gccaaaagtg atttggtatt gtaattgtta
     2281 tgtcgtgatt atgagaaatt taaaacaact aatgtttcga ggatttataa acgctttaat
     2341 tgcttttttt tgcggaggta cagcttcggc acaatcgcca caaacacctg aaattaaccc
     2401 tatgaacgtg ttgtttatcg ggaatagttt tacacacatg aacagcatgc cggtaatgtt
     2461 tgaacgtttg gcaatttcaa aaaatattaa gataaacgtt ttgatgagcg ctaaaagtaa
     2521 ccacacattt gagatgcatt ctaaacgtgc tgatttatac gaagacattc ataaagcaaa
     2581 atgggattac gtggtattgc aaggatttag tcgcgagttt agttacggcc aagagcacat
     2641 cgacacagca gttattcctt attttcaaca aatagttgac accttgtatc aaaacaatcc
     2701 gtgtgtgaag ttattgttgt acatgacttg gggatacgaa aacgggtatg cgtatatgaa
     2761 tgaaattggt acgtatgaag aaatgagtgc tgccattgaa agtggatacc gttatattgc
     2821 tggaaaatac aacttggcta tagttcc
//
LOCUS       DAOQVQ010000304         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_41637_length_2842_cov_3.370649, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000304 DAOQVQ010000000
VERSION     DAOQVQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41637_length_2842_cov_3.370649"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="PKN22_08755"
     CDS             <1..1873
                     /locus_tag="PKN22_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNS42834.1"
                     /translation="KPVGHGRAIERFDGDMLVQQEPDASSFPNGGIRNTFEARGYTAW
                     DPSSPAFVIGKTLCIPCVFISYTGESLDFKMPLLKALHAVEKAAVDVCQYFDKNVTKV
                     VATLGWEQEYFLIDQAYYNARPDIMMTGRALFGQISAKGQQLDDHYFGTIPSRVTAFM
                     REFETESVRLGIPVTTRHNEVAPNQFECAPIFEECNLANDHNQLLMDIMNKVAIKHDF
                     RVLFHEKPFAGVNGSGKHNNWSLSTNTGVNLLAPGKNPKSNLQFITFFVNTIKAVHDN
                     ADLLRASIASSTNDYRLGANEAPPAIISMFIGTYMNDLLDELEKNIKAGKMTPEAKTE
                     LKLNIGKIPQILLDNTDRNRTSPVAFTGNKFEIRGVGSSANCGHPMFVMNTIMAKQLK
                     EFKIAVDKRIEKGDGKDEAILKELQKLIKESKKIRFEGNGYGEEWVKEAAKRGLSNHK
                     DTPKALEVWGEKWVSDLFEEMEVLSKRELLARQENEYESYVMKVQIESRILNELSQNQ
                     ILPAAVEYQNKLLLNVRELKELLGEKAGKEAAASQIKIIEKIATHLNSLISLNFEMLE
                     ARKVANKIEDYHKRAFAYCDTVKVHFDKIRYHADKLELLIEDELWPLPKISEMLFTK"
     gene            complement(2064..2435)
                     /locus_tag="PKN22_08760"
     CDS             complement(2064..2435)
                     /locus_tag="PKN22_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42835.1"
                     /translation="MKNALKKAGLFSLMLTFVAFTNLGCRKKKDTIAEITVKNSAGAV
                     VSGASVRLFPVPTQQGTLLWEFETTTNSDGVASFNFNDIYQLGQAGVVVANIEATANG
                     TTGKGVIKVDQETTSTATVFL"
     gene            complement(2453..>2842)
                     /locus_tag="PKN22_08765"
     CDS             complement(2453..>2842)
                     /locus_tag="PKN22_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42836.1"
                     /translation="VINTMKTLKSILPIVTILLSVTLLYSCKDKTPSVAKIFVRSNSN
                     ELLQDARVVIIADISKNDSDIEYVDTLMTNSSGFAEFNLQDYYNQVGKEVTVATFDVI
                     CKKAELTGEDEIRTRIHTTAVETIYVK"
BASE COUNT          945 a          455 c          558 g          884 t
ORIGIN      
        1 taaaccagtt ggacacggta gagcaattga gcgttttgat ggagatatgt tggtacagca
       61 agaacctgac gcatcgtcgt ttccaaatgg tgggattaga aatacatttg aagcgagagg
      121 ttacacagca tgggacccat cttctcctgc gtttgtaatc ggtaaaacgt tgtgtattcc
      181 atgtgtattt atttcctaca caggtgaatc gcttgacttt aaaatgccat tattgaaagc
      241 attgcacgca gttgaaaaag cagcagtaga tgtttgtcag tattttgata aaaatgttac
      301 aaaagttgta gcaactttag gttgggagca agaatacttt ttgattgatc aggcttatta
      361 caatgcacgt ccggatatta tgatgacagg tagagctttg tttggacaaa tttctgctaa
      421 aggacaacaa ttagatgatc attattttgg aacgattcca tcacgtgtta cagcttttat
      481 gcgtgagttt gaaactgaat ctgtacgttt aggaattcct gttacaaccc gtcacaacga
      541 ggtggctcca aaccaatttg aatgtgcacc aatttttgaa gagtgtaact tagcaaatga
      601 ccacaaccaa ttgttgatgg atattatgaa taaagttgct atcaaacacg attttagagt
      661 gttgttccac gaaaaaccgt ttgctggggt aaatggttct ggaaaacaca ataactggtc
      721 gttaagcacc aacacaggtg ttaacttatt ggcaccagga aaaaatccaa aatccaactt
      781 acaatttatt accttttttg tgaacaccat taaagcggtt catgataatg cagatttgtt
      841 aagagcatcc attgcatcgt caacaaatga ttaccgtttg ggagccaacg aagcaccgcc
      901 tgcaatcatc tccatgttta tcgggacata catgaacgat ttgttggatg aattggagaa
      961 aaacatcaag gctggtaaaa tgacgcctga agcaaaaaca gagttgaaat taaatattgg
     1021 taaaattcca caaattttgt tggacaatac tgatagaaat agaacttcgc cagttgcctt
     1081 tactggaaat aaatttgaga ttagaggcgt tggttcttct gcaaactgtg ggcatcctat
     1141 gtttgtgatg aataccatta tggcaaaaca attgaaagaa tttaaaattg cagttgataa
     1201 acgtattgaa aaaggtgatg gtaaagacga agcgatttta aaagaacttc aaaaattaat
     1261 caaagagtct aaaaaaatcc gttttgaagg aaatggttat ggtgaagaat gggtgaaaga
     1321 agctgcaaaa cgcggattat ctaaccataa agatacacca aaagcacttg aagtttgggg
     1381 tgaaaagtgg gtgtctgact tgtttgaaga aatggaagtg ttgtcaaaaa gagagttgtt
     1441 ggcacgtcaa gaaaacgagt atgaaagtta tgtgatgaaa gtgcaaattg aatcacgtat
     1501 tttgaacgag ttgtcgcaaa atcaaatctt accagcagca gttgaatacc aaaacaaatt
     1561 gttgttaaac gtgcgtgaat taaaagaatt gcttggcgaa aaagctggta aagaagccgc
     1621 tgcttctcaa attaaaatca tcgaaaaaat tgcaacacat ttgaacagtt tgatatcgtt
     1681 aaattttgaa atgttggagg cacgcaaggt ggcaaataaa attgaagatt accacaaacg
     1741 tgcatttgca tattgcgata cagtaaaagt tcactttgac aaaattcgtt atcacgctga
     1801 taaattagag ttgttgattg aagatgaatt gtggccgtta ccaaaaatca gcgaaatgtt
     1861 gtttactaag taattgcttc atttaacaga aaagcaagcg gatgaattga ttgattcatc
     1921 cgcttttttt atgtcttatt ttggaaaaga tagcgttttt gtgttcttat cccaacaaca
     1981 ttgatttaag aattttgtat tgtttagcaa taaaaaatcc gaccctgaat agagtcggat
     2041 ttactatttt gaattattgt ttcctataag aatacagttg ctgtagaagt tgtttcttga
     2101 tctactttta taaccccttt accagttgtt ccgttagctg ttgcttcgat gtttgcaacc
     2161 acaacaccag cttgtcccaa ttgataaatg tcgttgaagt taaagctagc aactccgtca
     2221 gaattggttg tagtttcaaa ctcccataat aaagtacctt gctgtgttgg aacagggaaa
     2281 agacgaacgc ttgcaccaga aacaactgca ccagcagaat ttttaacggt gatttcagca
     2341 atcgtatctt ttttctttct acaccccaag ttagtaaatg caacaaaagt gagcattaaa
     2401 ctaaacaaac cagctttttt aagtgcattt ttcatgttct tcaattttaa agttatttta
     2461 catatatagt ttcaacagca gttgtgtgaa tacgcgtgcg aatttcatct tcacctgtca
     2521 attctgcttt tttgcaaatt acatcaaatg tagcaactgt aacttctttg ccaacttgat
     2581 tgtaataatc ttgcaagtta aattcagcaa aaccagaact gttagtcatt aatgtatcta
     2641 cgtattcaat gtctgaatcg ttttttgaga tgtcagcaat gataacaaca cgtgcatctt
     2701 gcaaaagttc attgctatta ctgcgtacaa aaattttggc tacagaaggt gttttgtcct
     2761 tgcaagaata aagtaaagta actgataata aaatagttac tattggtaag atgcttttta
     2821 atgttttcat tgtattaata ac
//
LOCUS       DAOQVQ010000305         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42059_length_2822_cov_3.147452, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000305 DAOQVQ010000000
VERSION     DAOQVQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42059_length_2822_cov_3.147452"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKN22_08770"
     CDS             complement(<1..1010)
                     /locus_tag="PKN22_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42837.1"
                     /translation="MNFLKSKWFKIPYTIALYGFAAYGFFLVLTYLAMKFQWTKEGGM
                     VDKNNRYFQSMHDKYNQGFKVDSVSVSKRRHEILNRIILLNDYYPVNAEYIFSAYEKS
                     KDDKIALQMLDAVELQLVDNKKYQKERRELNAKLNSNTQSTGLSAFKWMNVQEWKYFK
                     EALVKDKKWIDSAAVVSGVSPRLIVACLVGEQVRLFNSRRERFKEYIAPLKTLALETN
                     LSFGVTGIKESTAIKIENYLKDKNSPFYLGEEYENVLDYDSLANYTNNLNDTMSVRLQ
                     RLVQFSNHYYSYLYAALYVRQINAQWERAGFPISYRPEIFASLFNLGYQKSKPKKNPE
                     AGG"
     gene            1139..>2822
                     /locus_tag="PKN22_08775"
     CDS             1139..>2822
                     /locus_tag="PKN22_08775"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNS42838.1"
                     /translation="MKKTLLLIGGLVLLNSAVFSQIFTDNFDSYTAGDFLVSSNPVDW
                     STWSGGAGGSTEDTKISSTQAASAPNSVYFSSTAANGGPSDIILKFDEVYTSGVFTLE
                     ANYFVESGKGAYFNLQQDHTPGNIWALDCYMNNAGTIKFKGNNNSVTLLTGTYPTNTW
                     FNLKLTIDLTSNLWEVFIDGTSIGSFSNAISSIGIWDIYPVNPTGEGGNNQAGFYIDD
                     VSYSHTPASLLPLNAGVSYVEQVEGLVGQTGSVTAKVRNLGTTAITSFDLEYTYNGNT
                     VTESITSVNIASLAFHNYTFTAPITLIAGNNPLTVKVKNVNGNANDDDATDDAKTVNL
                     NPVVPTAGKIVVGEEGTGTWCGWCPRGAVFMDMMADKYDGFWAGIAVHNGDPMVDAVY
                     DAGMATKVSGYPSALVDRGTTIDPSQMETDFITRITQPIKAVLTNGATFNSTTRELKV
                     SVKYVFNGTVPTSWKVACALTEDDVTGSGSGYNQANYYTGGGSGVMGGFETLPNPVPA
                     AQMVYNHVARKIEPSFAGGSGLLPSGITAGTEHTVCFTFILPATWDETKMHIV"
BASE COUNT          887 a          487 c          516 g          932 t
ORIGIN      
        1 ccacctgctt ctggattttt cttaggtttt gatttctgat aacctaaatt aaacaacgat
       61 gcaaaaattt ctggtcggta actaattgga aatcctgctc tctcccactg cgcattaatt
      121 tgacgcacat ataatgcagc atataaatag gaataataat ggttagaaaa ttgcaccaat
      181 cgttgcaaac gaacgctcat cgtatcatta agattgttgg tataattggc caaagaatca
      241 taatccaata cattttcata ttcttcgcct aaataaaacg gagaattttt gtctttcaaa
      301 taattttcaa tttttatggc cgtactttcc ttaattccag taacaccaaa ggataaattg
      361 gtttctaaag ccaatgtttt taatggtgca atgtattctt taaaacgttc tctacgcgaa
      421 ttaaacaagc gtacttgctc acctaccaaa caagctacaa tcaaacgtgg agatacaccc
      481 gaaacaacag ctgcagaatc aatccatttt ttatctttaa ccaatgcttc tttaaaatat
      541 ttccattctt gcacattcat ccatttaaac gcactcaagc cagttgattg ggtattggaa
      601 tttaattttg cgtttaattc tctacgttct ttttggtatt ttttattatc aaccaattga
      661 agttcaacgg catccaacat ttgaagtgca attttatcat cttttgattt ttcataagca
      721 ctaaaaatat attcggcatt tacagggtaa taatcgttca ataaaatgat gcgattcaaa
      781 atttcatggc gacgtttaga aactgaaaca ctgtctactt taaatccctg gttatattta
      841 tcgtgcatgg attgaaaata acggttgttt ttatccacca ttccaccttc ttttgtccac
      901 tgaaatttca tcgccaaata ggtcaacacc aaaaagaaac cataagcagc aaagccgtat
      961 agagcaattg tataaggaat tttaaaccat ttacttttta aaaaattcat cgcacttttg
     1021 ttaaagaatt cgcaaaatta atgaataaag aattgtattt cacattcttc aactgtttta
     1081 ttttattttg attttttttc ttaactttcg tttttattat gaaactaaat ttaatagtat
     1141 gaagaaaaca ttacttttaa taggtgggtt agttttactc aactcagctg ttttttctca
     1201 aatttttacc gataattttg acagctatac tgctggtgat tttttggtaa gttccaaccc
     1261 tgttgattgg agtacttggt ctggtggtgc tggtggaagt actgaagata caaagatttc
     1321 tagcacacag gcagcaagtg cacccaactc ggtgtatttt agctctacgg ctgcaaatgg
     1381 tggtccatcc gacattattt tgaaatttga cgaagtatat acttctggtg ttttcacact
     1441 agaagccaac tattttgttg aatctggtaa aggagcttat tttaacttgc agcaagacca
     1501 tacacctgga aacatttggg cgttggattg ttatatgaac aatgctggaa caatcaagtt
     1561 caaaggaaac aacaattctg taacgttgtt aaccggaact tatccaacca atacttggtt
     1621 taatttgaaa ttaactattg atttgactag caatttatgg gaggttttta ttgatggtac
     1681 aagcatcggt tcgttttcaa atgccatttc atcgattgga atttgggata tctatcctgt
     1741 aaatccaact ggtgaaggag gaaataatca agcaggattt tacattgatg atgttagtta
     1801 tagtcacaca cctgcttcac tattaccttt aaatgcgggt gtttcttacg tggagcaagt
     1861 tgaaggattg gttggtcaaa caggaagtgt aactgcaaaa gtgcgtaact taggtacaac
     1921 tgctattacc tcttttgatt tagaatacac ctataatgga aacactgtaa cagaatccat
     1981 cacaagtgta aacatagcaa gtttggcatt tcataattat acatttacag caccaatcac
     2041 tttaattgct ggtaacaatc cattaactgt taaggttaaa aatgttaatg gtaatgcaaa
     2101 cgacgatgat gctactgatg atgctaaaac agtgaatttg aatcctgttg ttccaactgc
     2161 tggaaaaatt gttgtaggtg aagaaggaac aggtacatgg tgtggatggt gtccacgcgg
     2221 tgctgtattt atggatatga tggcagataa atacgatggt ttctgggctg gtattgccgt
     2281 tcacaatgga gacccaatgg tagatgctgt ttatgatgct ggtatggcta caaaagtttc
     2341 tggctatcca agtgctttgg ttgaccgtgg aacaacaatt gatccatcac aaatggaaac
     2401 agattttatt acacgtatta ctcaaccaat taaagctgtt ttaacaaatg gagcaacatt
     2461 taatagcaca acacgcgaat taaaagtgag tgtaaaatat gtgtttaatg gaactgttcc
     2521 tacatcgtgg aaagtggctt gtgcattaac agaagacgat gtaactggaa gtggtagcgg
     2581 ttacaaccaa gcaaactatt atacaggagg tggtagtggt gttatgggtg gttttgaaac
     2641 tttacctaac cctgtaccag cagcacaaat ggtatacaac cacgtagctc gtaaaattga
     2701 accttcattt gctggtggct caggtttatt accttctggt ataacagctg gaactgaaca
     2761 tacggtgtgt tttacattca ttttacctgc aacatgggat gaaacaaaaa tgcacattgt
     2821 gg
//
LOCUS       DAOQVQ010000306         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42098_length_2820_cov_3.586980, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000306 DAOQVQ010000000
VERSION     DAOQVQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42098_length_2820_cov_3.586980"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1782
                     /gene="dnaB"
                     /locus_tag="PKN22_08780"
     CDS             220..1782
                     /gene="dnaB"
                     /locus_tag="PKN22_08780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNS42839.1"
                     /translation="MENNQSENKRKPAARISSSSPIINELGKVPPQALDLEQAVLGAM
                     MLEKNAVIDTIEILMVESFYDPKHQYIYGAIRELFGTSNPIDILTVVNLLKKKGELAA
                     AGGAAYVSSLTSKISSTANVEYHARIIAEKFIKRELIRMSSEVIKNAYDDTNDVFQVL
                     GDAEENLFKIAENNFGKAVDTMPNVVRMAIEEIENAAKESSGISGIPTGLFELDKITS
                     GWQRSDMIVIAARPAMGKTAFVLSMARNTAVDYGKGVAIFSLEMSSVQLVKRLIASES
                     RLSAEKLRKGDLAEHEFQQLHTRISKLAKAPLYIDDTPGISVFDLRAKCRRLKMQYNI
                     DMVIIDYLQLMTVGGPKGSGNREQEISTISRSIKEIAKELNVPIIALSQLSRSVETRG
                     GDKKPVLSDLRESGAIEQDADIVSFIYRPEYYNIDFDEKGESNKGIGEIIIAKHRNGG
                     LGTVRLRFIGEYARFENLNEFNDFDLDNKTSALNTASRSENFITIPSKMNAFDNSGSD
                     FDRSFDDDSIPF"
     gene            1789..>2820
                     /locus_tag="PKN22_08785"
     CDS             1789..>2820
                     /locus_tag="PKN22_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNS42840.1"
                     /translation="MKNFCFIVFLFIGFHLKAAQLLVPMDDSQSNHLKAYGVAYWSLQ
                     HEVVIEWLLNYRGGSFLFPHLKSVEQELIIRGIKYEILSDGQANAIRNKISNPEENME
                     IVQLEKAPKIAVYTPDGKMPWDDAVTLVMEYAEIPYDKIYDSAIVNGKLHEYDWLHLH
                     HEDFTGQYGKFYAAYRSYPWYRQQVADAEAMAARLGYSKVSQLKLAVAQSIKNFVLGG
                     GFLFTMCSGTDSFDIALAAQNTDICEQMYDGDPASPNCQKELDFNQTLAFKDFLLERD
                     PLKYEYSSIDGTELRRTQRVQETVDKFTLFEFSAKWDIVPTILTQNHTTIINGFMGQT
                     TAFNKDCIKP"
BASE COUNT          894 a          475 c          598 g          853 t
ORIGIN      
        1 gttttagaac aaattcacga ccaattggtt tccatcgaca tcaatacatt gactcctgta
       61 gaagctttaa tgaaattaaa cgacatcaaa aagttaatag gcggataatg aggtgttttt
      121 gcgattatta acaattgtta ataactgcta tggatatctt ttaatgcact tttttgattt
      181 tgatttcatc taaggttttt gtttgcttat atttgtatta tggaaaataa tcaatcggaa
      241 aataaacgaa aaccagcagc gcgcatttct tctagttctc ctattatcaa tgagttgggc
      301 aaagttccgc cacaagcgtt ggacttagaa caagctgttt taggggcaat gatgttggaa
      361 aaaaatgcgg tgatcgatac cattgaaatt ttgatggtcg aaagttttta tgatcctaaa
      421 catcaataca tttatggtgc cattcgtgag ttgtttggaa cttccaatcc gattgatatc
      481 ctgacagttg ttaacttact taaaaagaaa ggtgaattag cagcagcagg aggagcagcc
      541 tatgtttctt cgttgactag taaaatttct tcgactgcaa atgtggaata ccatgcccgt
      601 attatagctg agaaattcat aaaacgcgag ttgattcgca tgagttcgga agtgattaaa
      661 aatgcttacg acgacaccaa cgatgtgttt caagtgttgg gtgatgccga agagaacttg
      721 tttaaaattg ctgaaaacaa ctttggtaaa gccgttgata cgatgcccaa tgtggtgcgt
      781 atggctattg aagaaattga aaatgcagca aaagaaagca gtggtatttc tggaattcca
      841 accggtttgt ttgagttgga caaaattacc tcgggttggc aacgttccga tatgattgtt
      901 attgcagcgc gcccagcaat gggtaaaacc gcttttgtat tgtcgatggc acgcaacacc
      961 gctgttgatt acggaaaagg agttgcaata ttctcattag agatgtcatc tgttcaattg
     1021 gttaaacgtt tgattgcaag tgaatcgcgt ttaagtgccg aaaaattgag aaaaggtgat
     1081 ttagcagaac acgaatttca acagttgcat acccgtattt caaagcttgc aaaagcacca
     1141 ctttatattg acgacacacc gggaatttcg gtttttgacc ttcgtgcaaa atgccgtagg
     1201 ttgaaaatgc aatacaacat cgatatggtg attatcgatt acttgcaatt gatgactgtt
     1261 ggtggaccta aaggaagtgg aaatagagag caagagattt caaccatttc tcgctccatc
     1321 aaagaaattg caaaagaatt aaatgtgcca attattgcac tttcgcagtt gagtcgttcg
     1381 gtagaaacac gtggtggtga taaaaaacct gtactttccg accttcgtga gtcgggtgcc
     1441 attgagcaag atgctgatat tgtttcgttt atttaccgcc cagaatacta taacatagat
     1501 tttgatgaaa aaggagaaag taataaaggc attggagaaa tcattattgc taaacaccgt
     1561 aatggtggtt taggaacggt tcgtttgcgc tttattggtg aatatgcacg ttttgaaaac
     1621 ttaaatgaat tcaatgattt tgatttagac aataaaacat ctgcattaaa tacggcgtca
     1681 agatcggaga atttcattac tattccatca aaaatgaatg cttttgataa tagcggctct
     1741 gattttgata gatcgtttga tgatgatagc ataccatttt aaaagcacat gaaaaatttt
     1801 tgttttattg tatttttgtt cattggattt cacctgaaag cagcacaatt gctggtgcca
     1861 atggatgata gccaatccaa tcacttgaaa gcttatggcg ttgcatattg gtcgttgcaa
     1921 cacgaagtag tgattgaatg gttgctcaac tatagaggtg gttcttttct ttttccgcat
     1981 ctaaaatcag tagaacaaga gctaattatc agagggataa aatacgagat tctttcagac
     2041 ggacaagcca atgcaattcg caataaaatc tccaatccag aagaaaatat ggagattgtg
     2101 caactggaaa aagcacccaa aattgctgtt tacacgcccg atggaaaaat gccgtgggac
     2161 gatgccgtta cattggtaat ggaatatgct gaaattcctt acgataaaat ctacgattca
     2221 gcaattgtga acggcaagtt gcacgaatac gattggttgc acttgcacca cgaagatttt
     2281 acaggtcaat acggtaagtt ttatgcagct tatcgttcgt atccttggta ccgtcagcaa
     2341 gttgccgatg cagaagctat ggcggcacgt ttgggatatt caaaagtttc acaactaaaa
     2401 ttagcagtgg cgcaaagcat aaaaaacttt gtactcggag gtggcttttt gtttaccatg
     2461 tgtagtggta ccgatagttt tgatattgct ttagcggcac aaaacacgga tatttgtgag
     2521 caaatgtatg atggtgaccc tgcaagtccc aattgtcaaa aagaattgga tttcaaccaa
     2581 acgttggcat ttaaagattt tttattggaa cgcgacccat tgaaatacga atattcgtca
     2641 attgatggaa cagaattaag aagaacacaa cgtgtgcaag aaaccgttga caagtttacg
     2701 ctctttgagt tttctgccaa atgggatatt gtgcctacta ttttaacaca aaaccataca
     2761 acaattatca atggttttat ggggcaaacc actgctttta acaaagattg cattaaaccc
//
LOCUS       DAOQVQ010000307         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42126_length_2819_cov_3.294501, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000307 DAOQVQ010000000
VERSION     DAOQVQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42126_length_2819_cov_3.294501"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1562)
                     /gene="clpX"
                     /locus_tag="PKN22_08790"
     CDS             complement(330..1562)
                     /gene="clpX"
                     /locus_tag="PKN22_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNS42841.1"
                     /translation="MAKKDLSCSFCGRSREEAGELLSGVAGHICPNCVEQAHQIVLAE
                     FGRQSKKSSPKMKMNVLKPMEIKSHLDEYVIGQDDAKKVLSVAVYNHYKRLNQPETAD
                     EVEIEKSNVILVGRTGTGKTLLAKTIAKMLNVPFCIADATILTEAGYVGEDVESILSR
                     LLQAADYDVEAAEKGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGAIVNV
                     PPQGGRKHPEQKMIQVNTKNILFVCGGAFDGIERIIANRVNRQTIGFSSSDEEQVDGE
                     NLLKYVIPTDIKTFGLIPELIGRFPVLTYLQPLDAAALKRILVEPKNALVKQYEKLFD
                     LDGISLSFDAKVLDFIVEKALEFKLGARGLRSICEAILNDAMFEMPTSKEKELKITLD
                     YAKAKFDKSKIAQLKIAI"
     gene            complement(1636..2316)
                     /gene="clpP"
                     /locus_tag="PKN22_08795"
     CDS             complement(1636..2316)
                     /gene="clpP"
                     /locus_tag="PKN22_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNS42842.1"
                     /translation="MFDGNEFKKYATKHMGISSMHVSNYIDSSFKNMTPYIIEERPLN
                     MTQMDVFSRLMMDRIIFLGTGIDDQVANIINAQLLFLESVDPKKDIQIYLNSPGGSVY
                     AGLGIYDTMQYVRPDIATICTGMAASMGAVLMCAGAEGKRSALKHARVMIHQPLGGAQ
                     GQASDIEITAREIQKLKKELYEIIAHHSKQPYEKVWEDSDRDYWMTAEEAQAYGMVDE
                     ILVRNPQK"
     gene            complement(2356..>2819)
                     /locus_tag="PKN22_08800"
     CDS             complement(2356..>2819)
                     /locus_tag="PKN22_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS42843.1"
                     /translation="EAHYLSKLCKKVTMLVRRDEFRASKIMADRVKNTPNIEILFNTE
                     TVDVLGDGNVVTGVKVVNNQTNETKEIPCTGFFVAIGHQPNTEIFKDYINLDETGYIK
                     YEKPGTSLTNVPGVFVAGDAADRHYRQAITAAGTGCMAALDAERYLAALEG"
BASE COUNT          820 a          566 c          465 g          968 t
ORIGIN      
        1 aggaaaccat tgcaagtctt cccattcaag tgcatgcctt cttttcatat taaagcaaat
       61 tttgaatttc aaatatctga aaaaactttg tttaacgagt attcaatagt ttaaatttta
      121 ataggttgcg tcaatgttaa caagttgaaa gtggattttt gaaattttat acgttacatc
      181 gtattttact tcaattccta tttgttttgt gcgttatccc agaaaaataa tagtgctaca
      241 tggtggaact catgtcttaa aaacaaaaat taccaaaact tgcagttaac aggttttggt
      301 aattctatga gtattgaaaa aaatatgaat tagattgcaa tttttaattg tgcaattttt
      361 gatttatcaa attttgcttt ggcataatct aatgtaattt ttaattcttt ttctttgctt
      421 gttggcattt caaacatggc gtcgttgaga atggcttcac aaatagaacg caatcctctg
      481 gctcctaatt taaactcaag tgctttttca acaatgaaat ccaatacttt ggcatcaaaa
      541 gaaagtgaaa taccatccaa atcaaacaat ttttcgtatt gttttaccaa ggcattttta
      601 ggttcaacca aaatacgttt caatgcagca gcatccaacg gttgcaaata cgttaaaact
      661 ggaaaacgtc caattaattc gggaattaat ccaaaagttt taatatctgt tggaatgacg
      721 tatttcaaca agttttcacc gtccacttgt tcttcatccg acgacgaaaa accaatagtt
      781 tgacgattta cacggttggc aataatgcgc tcgattccat caaaagcacc accacaaaca
      841 aacaaaatat ttttggtgtt gacttgaatc attttttgct ctggatgctt tcttcctcct
      901 tgtggcggaa cgtttacaat tgctccttcc aataatttca acaaggcttg ttgcacacct
      961 tcaccagaaa catcacgggt aatggatgga ttgtcgcttt tacgtgcaat tttatcaatt
     1021 tcatcaatga aaacaatgcc tttttcggct gcttctacgt cgtaatctgc ggcttgcaat
     1081 aaacgggata aaatggattc cacgtcctca ccaacataac ctgcttcggt taaaattgta
     1141 gcatcggcaa tacaaaacgg cacattcaac atttttgcaa tggttttagc caataatgtt
     1201 ttacccgttc ctgtgcgacc aaccaaaata acgtttgatt tttcaatctc cacttcatca
     1261 gctgtttcag gttggttcaa tcgtttgtag tgattgtaaa ccgctaccga aagtactttt
     1321 ttagcgtcgt cttgaccaat gacgtattca tccaaatggg atttaatttc cattggtttc
     1381 aatacattca ttttcatttt tggagaagat tttttggatt gtcgtccaaa ttcagccaaa
     1441 acaatttgat gcgcttgctc cacacaattt gggcaaatat gccctgctac tcctgataaa
     1501 agctcacctg cttcttctct tgatcgtccg caaaacgaac aacttaaatc tttttttgcc
     1561 atttatgggg tctaaaaatt aaaaatgagt gcaacactac tgctgcactc tattttttta
     1621 tctgttgttt aaaaattatt tttgaggatt tctcaccaaa atttcatcca ccattccgta
     1681 agcttgcgct tcttcggcag tcatccagta atctctgtca gaatcttccc acactttttc
     1741 atacggttgt ttagagtggt gtgcgataat ttcgtacaat tcttttttca atttttgaat
     1801 ttcacgtgct gtaatttcga tgtcggatgc ttgcccttgt gcacctccca atggttggtg
     1861 aatcattaca cgagcatgtt tcaacgccga acgttttcct tcagcacctg cacacattaa
     1921 aacagctccc atcgatgcgg ccattcccgt acaaattgtt gcgatatctg ggcgtacgta
     1981 ttgcatggtg tcgtaaattc ctaatccagc atataccgaa cctcctggag agttcaaata
     2041 aatttgaatg tcttttttag gatctacaga ttccaagaat aacaactgtg cgttgataat
     2101 atttgccact tgatcatcaa ttcccgttcc taagaaaata atgcggtcca tcatcaaacg
     2161 cgaaaaaacg tccatttgag tcatgttcaa tgggcgctcc tcgatgatgt aaggcgtcat
     2221 gtttttaaat gaagaatcga tgtagtttga aacatgcatg gagctaattc ccatgtgttt
     2281 cgttgcatat tttttaaatt cgtttccgtc aaacatttgc tataaatttt gtttaaagtt
     2341 agtataaata attgattatc cttctaatgc ggctaaataa cgttcagcat ccaaagcagc
     2401 catacaacct gttccagcag cagtaattgc ctgacgatag tgtctgtctg cagcatcacc
     2461 ggcaacaaaa acaccaggaa cattggtcaa cgaagttcca ggtttctcgt atttaatgta
     2521 accagtttcg tctaaattga tatagtcttt gaaaatctct gtatttggtt ggtgtccaat
     2581 agctacaaag aagcctgtac aaggaatttc ttttgtttcg ttggtttgat tgttaaccac
     2641 tttaacacct gttacaacgt tgccatcgcc caatacgtcc actgtttctg tgttgaataa
     2701 aatttcaatg ttaggtgtgt ttttaacacg atcagccata attttagaag ctctaaattc
     2761 atcgcgacgt accaacattg ttactttttt acacaatttt gacaagtaat gtgcttctt
//
LOCUS       DAOQVQ010000308         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42153_length_2818_cov_3.564604, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000308 DAOQVQ010000000
VERSION     DAOQVQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42153_length_2818_cov_3.564604"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKN22_08805"
     CDS             <1..265
                     /locus_tag="PKN22_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNS42844.1"
                     /translation="GLTGPTLETPAEYKYVRAIGADAVGMSTVPEVIVARHMNIPCFA
                     ISIITDLGVPGKIKEVSLQDVIDVANRQEPKMTLIMRELITRI"
     gene            310..1755
                     /gene="gatB"
                     /locus_tag="PKN22_08810"
     CDS             310..1755
                     /gene="gatB"
                     /locus_tag="PKN22_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNS42845.1"
                     /translation="MNEKYEAVIGLEVHAQMLTKTKAYSNDINEYGAQPNTNVSVVSL
                     AHPGALPMMNKKTIEYAIRLGLACHCNIADYQYFARKNYFYPDLPKGYQITQDKTPIC
                     TGGAIIIKDENGNDKSIGLTRIHMEEDAGKSIHDVDVYDTLVDLNRAGVPLLEIVSEP
                     DIKTSQEAYNYLTEVRKLVRYLDICDGNMEEGSLRCDANISVMLKGSKVFGTKVEVKN
                     MNSIRNVQRAIEFEINRQIEALEKGEEIVQETRSFDALKGSTISMRSKEAANDYRYFP
                     EPDLQPLFVGKEQIEVVKNEMPPLPRDLFIKYTKELGLSDYDAYVLTDTKEVALYYEG
                     VIAHTKNYKAAANWVMGDVKSYINHRGLEITDFPVLPQQLAALIQLIDEGKISTSIAS
                     QKVFPALTENPTEAPLKIAEQLNVIQDSNADSIKEYILKVIELNPNEVARYKNGEKQL
                     TGFFMGQLMKVSQGKADPKQANQLMREMLEQ"
     gene            1813..>2818
                     /locus_tag="PKN22_08815"
     CDS             1813..>2818
                     /locus_tag="PKN22_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS42846.1"
                     /translation="MKHFLKKSNYSKLVVLFTLVIVTACNSSEKVDETANGKENNLFI
                     SGVISGAEGQGVFLEAASQNGVINVAETKITDGKFELATNIPGLGIYQLRLGDKSENA
                     LVLTANSGDKIKIEGDINTIALNAKISGVDWAKQYNEFMQLAANFSNAQNELMGMQGK
                     VTQEELVKRYLELKKPMDDYAKEQINKNPASAFNIVLSNSLMPMQGFIDYPKENVEIL
                     RKMADSYTEKYANSPVAQSLNEQVTQIESGYQEYLELKSGKKFAPDLIMKSPEGKEIK
                     LSSLKGKIVLVDFWASWCGPCRKENPNVVRLYNKYKNKGFTIYSVSLDTEKSSWVNAI
                     E"
BASE COUNT          991 a          480 c          570 g          777 t
ORIGIN      
        1 aggtttaaca ggaccaacat tggaaactcc agcagaatac aaatacgtgc gtgctattgg
       61 cgctgatgca gtaggaatgt caaccgttcc agaagttatc gttgcacgcc acatgaatat
      121 tccttgtttt gcaatttcta ttatcaccga tttgggtgtg cctggaaaaa tcaaagaagt
      181 aagtttgcaa gatgtaattg atgttgccaa tcgtcaagag ccaaaaatga cgttgattat
      241 gcgtgagttg attacacgta tttaatttaa aatcaacaga gtaggaagtc aatatttcac
      301 aatagaaaaa tgaacgaaaa atacgaagca gtaataggtt tagaagtgca tgcgcaaatg
      361 cttacaaaaa ccaaagcata ttcaaacgat atcaatgaat acggagcaca acccaatacc
      421 aatgttagcg ttgtgtcctt ggctcatcca ggtgctttgc ctatgatgaa caaaaaaacg
      481 attgaatatg ccattcgttt aggtcttgct tgccattgta atattgctga ttaccagtat
      541 tttgctcgta aaaattattt ctatcccgat cttccaaaag gatatcaaat tacacaagat
      601 aaaaccccaa tttgcactgg tggtgccatc atcattaaag atgaaaacgg caacgataaa
      661 tccatcggtc ttacacgcat acacatggaa gaggatgctg gtaaaagtat tcacgatgtg
      721 gatgtttacg acacgttggt ggatttgaac cgcgctggtg ttcccttgtt ggaaattgta
      781 tccgagccag atattaaaac ttcgcaagaa gcctataatt atttaaccga ggttagaaaa
      841 ttagtgcgtt acttggatat ttgtgatggc aacatggaag agggatcttt gcgttgcgat
      901 gccaatattt cggtaatgct caaaggctca aaagtatttg gaactaaagt agaggtaaaa
      961 aacatgaact ctattcgcaa tgtgcaacgt gccattgaat ttgaaatcaa tcgtcaaatt
     1021 gaggctttgg aaaaaggaga ggagattgtg caagaaacac gctcgtttga tgcgttgaaa
     1081 ggttctacta tttctatgcg ttccaaagaa gctgccaacg attaccgtta ttttccagaa
     1141 cccgatttgc aacctttgtt tgtaggcaaa gaacaaattg aagtggtgaa aaacgaaatg
     1201 ccaccactac cacgtgattt gtttatcaaa tacactaaag aacttggact ttcagactac
     1261 gatgcctacg tgttaaccga caccaaagaa gtggcgttgt actatgaagg agtaattgct
     1321 cataccaaaa attacaaagc agcagccaat tgggtaatgg gagatgtgaa atcatacatc
     1381 aatcaccgag ggttggaaat aactgatttt ccggtacttc cacaacaatt agcagccttg
     1441 attcaattga tagatgaagg gaaaatctct acatctattg cttctcaaaa ggtttttccg
     1501 gcattaaccg aaaatccaac agaggcaccg ttaaaaattg cagaacaatt gaatgttatt
     1561 caagattcca acgccgattc aataaaagaa tacattttaa aagtcattga attgaatcca
     1621 aacgaggttg cacgctataa aaatggagaa aaacagttga ctggattttt tatgggacaa
     1681 ttgatgaaag tttcgcaagg aaaagcagat cctaaacaag ccaaccaatt gatgcgtgaa
     1741 atgttggaac agtagtgtgt ttcaataagc aataattaat gattgtagta cgaaaaattt
     1801 ttaacagata aaatgaagca ttttttaaaa aagtcaaatt atagtaaact ggtcgtatta
     1861 tttacgttgg ttattgttac cgcttgtaat tcttcagaaa aagtggatga aaccgcaaat
     1921 ggaaaagaaa acaacttatt tatttcgggg gtaatttctg gcgctgaagg acaaggtgtt
     1981 tttttagaag ctgcatcgca aaatggcgta attaatgttg ctgaaacaaa aattacagat
     2041 ggaaaatttg aactggcaac caacattcca ggattgggaa tttatcaatt aagattggga
     2101 gataaatctg aaaatgcttt ggttttaacg gccaattcag gcgataaaat taaaatcgaa
     2161 ggcgatatca ataccattgc gttgaatgct aaaatttcgg gcgttgattg ggcaaaacaa
     2221 tacaatgaat tcatgcaatt ggctgcaaat ttcagcaatg ctcaaaacga attgatggga
     2281 atgcaaggca aagtaaccca agaagaattg gtgaaacgtt atttggaact taaaaaaccg
     2341 atggacgatt acgccaaaga gcaaatcaat aaaaatcctg caagtgcttt caatattgtg
     2401 ctttccaact cattgatgcc catgcaaggt tttatcgact atccaaaaga aaatgtggag
     2461 attttgcgca aaatggccga tagttacacc gaaaaatatg ccaattcacc cgttgctcaa
     2521 tcattgaacg agcaagtaac tcaaattgaa tcaggctacc aagaatattt ggaactaaaa
     2581 tcgggtaaaa aatttgctcc agatttaatc atgaaatcac ctgaaggaaa agaaatcaaa
     2641 ctttcttcgt tgaaaggaaa aatagttttg gttgactttt gggcatcgtg gtgtggacca
     2701 tgccgtaaag aaaatcccaa cgttgtgcgc ttatacaata aatacaaaaa caaagggttt
     2761 accatttatt ccgtttcttt ggataccgaa aaatcaagtt gggtaaatgc catcgaac
//
LOCUS       DAOQVQ010000309         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42172_length_2817_cov_3.561550, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000309 DAOQVQ010000000
VERSION     DAOQVQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42172_length_2817_cov_3.561550"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKN22_08820"
     CDS             complement(<1..517)
                     /locus_tag="PKN22_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS42847.1"
                     /translation="MPNLSHKATDMPASPIRRLVPFADKAQAKGVKIYHLNIGQPDIK
                     TPTTAITAMKNANIDVLEYSHSAGIASYRNKLAAYYQKIGINVTADDLIVTVGGSEAI
                     TFGFMTACNPGDEVIIPEPFYANYNGFAVMSEVKIVPVTAHIEDGFALPSIAEIEKKI
                     TPKTKAIVICNP"
     gene            655..1911
                     /locus_tag="PKN22_08825"
     CDS             655..1911
                     /locus_tag="PKN22_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS42848.1"
                     /translation="MDPYAALKYKEFRLFMVVRFALIFAWSMQFIIIEWQVYVMTKDP
                     FSLGLIGLAEIIPALSMALFAGHIVDQREKRNLLLLCIIAFSCISFGLFVLTLPQVLE
                     NYAQTTILIGIYCLIFLGGIVRSFIGPSIFSLVSLIVPKKVYPNAATWSSSVWQIGSV
                     GGPAIGGLAIHAFGVHWSLCFVFFCALVAIIFLVQIEKKPILNPKIGEPVLQSLKVGL
                     NFVFKTKEILGALSLDMIAVLFGGAVALLPLFAQDILHVGSQGFGFLRAAPAVGSFLT
                     LIATAYLPIAKNAGKKLLIAVFVFGLCIIAFGFSSIYWVSLVALFLSGVADGVSVVIR
                     QTILQLRTPENMKGRVSSVNSMFVGSSNELGTFESGLAAKLLGPVTAVVFGGVMTLLT
                     VGTTALISPTFRKLDLSKDIKENHQA"
     gene            1908..2672
                     /locus_tag="PKN22_08830"
     CDS             1908..2672
                     /locus_tag="PKN22_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNS42849.1"
                     /translation="MKKWLHISCFLMFIACSSQENQSVLVGTYQNEPPNADVPITVSK
                     KQCIDSIFATMNIVSIQSVDSSILVDAKYATTDNFMHQQLYEHIQTVYLQKDVAKRLA
                     KAQRYLKTLHPQWSLLVYDGTRPVDVQQRMWNALDSIPVHERVKFVSNPKNHSVHNYG
                     AAIDLTIVDERKVPIDMGAGYDDIRKIAYPSLEAQFLASGELTQVQVNNRILLRKVMK
                     SQGFINIPSEWWHFNAFRRDEVKQRYQVVLTEPSCE"
BASE COUNT          772 a          507 c          573 g          965 t
ORIGIN      
        1 ccggattgca aataacaatt gcctttgttt ttggagttat ttttttctca atttctgcaa
       61 ttgaaggtag tgcaaaacca tcttctatgt gcgcagtaac aggaacaatt ttcacttccg
      121 acatgactgc aaatccgttg taatttgcat aaaacggttc tggaataatc acttcatcac
      181 caggattgca cgctgtcata aatccaaaag ttatggcttc tgaaccacca acagtaacaa
      241 ttaaatcatc tgccgtaaca ttgatgccta ttttttggta atatgcagcc aatttgttgc
      301 gatacgatgc aattccagcc gagtgactgt attctaaaac gtcaatgtta gcatttttca
      361 tcgccgttat ggctgttgta ggtgttttta tgtcgggttg accaatgttt aaatggtaaa
      421 tctttacacc ttttgcttgt gctttatctg caaatggaac caatcttcta atcggagaag
      481 ctggcatatc tgtcgccttg tgtgataaat tgggcataac tctatttttt ttagactgca
      541 aagataggct tttaattggt tggattttta aaaagttgat aaaatgtcag cggtagtctc
      601 taaaatctta cttttgtaac acaatttatt tttagtgttg taaaatcatt caaattggat
      661 ccatacgccg cgctcaaata taaagaattt cgattgttta tggtggtgcg atttgcactc
      721 atctttgctt ggagcatgca atttatcatc atcgaatggc aagtttatgt tatgaccaaa
      781 gacccgtttt cgttggggtt gattggtttg gcagaaataa ttccggcact ttctatggct
      841 ttatttgctg gccatattgt ggatcaacgt gaaaaacgta atttgttgct cctgtgcatt
      901 attgctttta gttgcattag ttttggattg tttgtgttga ccttgcccca agtacttgaa
      961 aattatgcac aaaccacaat tttaatcggt atttattgtt tgatattttt aggaggtatt
     1021 gtacgctcat tcattggtcc aagtattttt tcgttggtat cgttgattgt gcctaaaaaa
     1081 gtgtatccca atgcggcaac gtggagcagc tcggtgtggc aaattggttc ggtgggcgga
     1141 cctgccattg gtggtttggc aattcatgca tttggcgtac attggtcact gtgttttgtg
     1201 tttttctgcg cacttgtagc catcattttt ttagtgcaaa ttgagaaaaa accgatttta
     1261 aatcccaaaa ttggcgagcc tgtattgcaa agtttaaaag taggattgaa ttttgttttt
     1321 aaaaccaaag aaattttagg agcgctatcg ttggatatga ttgcggtact ttttggtggt
     1381 gccgttgcat tgttgccttt atttgcacaa gatattttgc acgttggctc gcaaggattt
     1441 ggttttctgc gtgctgcacc agctgttggt tctttcctta cgttaattgc aactgcctat
     1501 ttgcccattg ctaaaaatgc aggaaaaaaa ttgctgattg ccgtttttgt ttttggtttg
     1561 tgcatcattg cttttggctt ttcatccatt tattgggttt ctttggttgc tttgttttta
     1621 agtggtgttg ccgatggtgt ttcggtggtt attcgccaaa ctattttgca attgcgcacc
     1681 ccagaaaata tgaaagggcg tgtttcttcg gtgaattcaa tgtttgttgg ttcatctaac
     1741 gaactcggta cttttgaaag tgggttggca gctaaattat tgggacctgt tacagctgtt
     1801 gtttttggag gtgtaatgac cttgctgact gtgggaacaa ccgctttgat ttcaccgact
     1861 tttaggaaat tggatttatc aaaagacatt aaagaaaatc atcaagcatg aaaaaatggt
     1921 tgcacataag ttgttttttg atgttcattg cctgttctag ccaagaaaat caaagtgttt
     1981 tagttggaac gtatcaaaac gaacccccta atgctgatgt tccaataacc gtttccaaaa
     2041 aacaatgtat tgattccatt tttgcaacca tgaacattgt gtccatccaa tcggttgatt
     2101 cttccatttt agtggatgca aaatatgcaa ctaccgataa ttttatgcac caacaattgt
     2161 atgagcacat acaaaccgtt tatttacaga aagatgtagc aaagcgattg gcaaaggcac
     2221 aacgctattt aaaaacacta catccacaat ggagtttgtt ggtttatgac ggaactcgcc
     2281 cagtggatgt gcaacaacgt atgtggaatg ctttggacag tattccagta cacgagcgcg
     2341 taaaatttgt ttccaatccc aaaaatcaca gcgtacacaa ttatggagca gccattgatt
     2401 taaccattgt agatgaacgg aaagttccta tagatatggg cgcaggttac gacgatatcc
     2461 gtaaaattgc ctatcccagc ttagaagctc aatttttagc atctggagaa ttaacccaag
     2521 tacaagtcaa caaccgcatc ttgcttcgaa aagtaatgaa aagtcaaggg tttatcaata
     2581 ttccatcaga atggtggcat ttcaatgcat ttagaagaga cgaagtaaaa caacgctatc
     2641 aagttgttct caccgaacca tcgtgtgagt agtttttgat ttggcggtga acgcgcaaac
     2701 tagcagtttt tccattgcgt tctaccatcc caaaccacct caaatcctac cttaccaccg
     2761 taataaaacc ttcttttgtt aaaaaaacgc aattgtaatt ttttaaagga ttcgagt
//
LOCUS       DAOQVQ010000310         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42208_length_2815_cov_3.394565, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000310 DAOQVQ010000000
VERSION     DAOQVQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42208_length_2815_cov_3.394565"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2743
                     /locus_tag="PKN22_08835"
     CDS             <1..2743
                     /locus_tag="PKN22_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42850.1"
                     /translation="SLDCHLSSASANYPVLADTLILDQGKLIANYDIYVRILLKLPSN
                     VPHSIKNGRKIQLQPAYSGVSSYLPSCPGGGTLSTTGAKAELNGSCASSFYITGGTFE
                     NNSPYPLNVDYAVITDNTVTGSNTPGISTNSILSGVTTGWTGAAAQARKLHWVNSSAT
                     VGDTGDFYDGNNWEQLLPFYAAAPQCPPTVIDTVLFDNTSFSAANQVVQLTGTPDVAS
                     MYWENIPSGMSPKLSGLATTQMSIRASLHFHDNMVNNQLGPFLFRGIPTTDFPAFTIR
                     GGTNKFLNKIEFLGDTDTTKWKLTDSLVQIYNNTFANTGYMYFKRGHIITNGKTVNVN
                     GYLSTGVLKRHLDMTNSIVKTKFPWQISGTNVGNTTAEYTQTSDGSTVIINPPNTFFN
                     EAFHIYFGGEKYNRVIANINCGNYGDLLSTNLTDSINYLEVNSINSKQYVGLKTVVKH
                     AVFNNNIVLVTDRGVYDTLIFNNNATINTSNKYNNLLHFEPGRSYTLQQSTVQWVQNN
                     AELELNGLSTATPIQFYGSPTGQQAYIRKDSGLVCADYVNIRDMWAIGNGNDAFGTCS
                     PNNYCTHTNPWIPSSCDTIVDLLSSCGPWTINPADHNRGRADFNGGEYADFQGGNTFG
                     WDKRAYPPAPLVSLTNLKDSICRMDSVAIQVSGVGTLPFIITWKENGVYHADTIYSAA
                     ELDSYNPTTSEFEIITWYYPMADISITDFAIKTERCLGSDSPVDNGVASITMLPCYLG
                     VELLDFKATLKNTDVLLNWSTASELNNDYFELQYTHDGVNWEVVDKVKGKGTTHTLNN
                     YSYVHYRPFGQSNTLYYRLKQVDFDGAFDYSPIRSVALNNGEGYVVYPNPATNVLYVS
                     GKGAFNYQMLDASGKVVAEGASSGQDEISLFQLNPGVYMLEITDDQGVKEIFKIVKI"
BASE COUNT          919 a          474 c          548 g          874 t
ORIGIN      
        1 ctctttagat tgtcatttgt cttcagcttc agctaattat ccggtgcttg cagatacgtt
       61 gattttagat caagggaaac taattgccaa ttatgatatt tatgtcagaa ttctattaaa
      121 attaccgagt aatgttccgc attcaataaa gaatggaaga aaaattcagc ttcaaccagc
      181 atatagtggt gttagctcct acttaccttc ttgtccaggt gggggaacac tttcaacaac
      241 tggagctaaa gcagagttaa atggttcttg tgcatcaagt ttttatatta ctggcggaac
      301 gtttgaaaat aattctcctt atcctttaaa tgtagattat gcggtaataa cagacaatac
      361 agttacgggt tctaacacac cgggaatttc tacaaactca attttgtcag gtgtaacaac
      421 gggttggact ggtgcggctg ctcaagcacg taaactacat tgggtaaatt catcggcaac
      481 agttggtgat actggtgatt tttatgatgg aaataattgg gaacaacttt taccttttta
      541 tgcagcagcc cctcaatgcc cacctactgt catcgatact gtattatttg ataatacctc
      601 attctctgca gctaatcaag tggtgcaatt aacgggaaca cctgatgtag catctatgta
      661 ttgggaaaat attccaagtg gcatgagtcc taaattaagt gggttggcta ctacacaaat
      721 gtcgataaga gcaagtttgc actttcacga taacatggta aataatcaat tgggaccttt
      781 cttgtttaga gggattccaa ctacagattt tcccgcgttt acaattcgtg gtggtacaaa
      841 taagtttcta aataaaatag aatttttagg tgatacagat actaccaagt ggaaattgac
      901 ggatagctta gtacaaattt acaataacac atttgcaaat acgggctata tgtattttaa
      961 aagaggacac atcataacta atggaaaaac tgtaaatgta aacggatatt tgtcaacagg
     1021 tgtgttaaaa cgccatttag acatgaccaa ttctattgtt aaaactaaat ttccttggca
     1081 gatttctgga acaaatgttg gtaatacaac agcagaatat actcaaacaa gtgatgggtc
     1141 aactgttatt attaaccctc caaatacatt tttcaatgaa gcttttcata tttattttgg
     1201 aggtgaaaaa tataaccgtg ttattgcaaa tattaattgt ggtaattatg gagatttatt
     1261 atctacaaat ttaactgatt caataaatta tttagaagta aactctatca attctaaaca
     1321 atatgttggt ttaaaaacag ttgtaaaaca tgctgttttt aataacaaca tcgttttagt
     1381 aacagataga ggagtttatg atacattgat tttcaataat aatgcaacca ttaacacctc
     1441 caacaaatac aacaacctat tgcattttga accaggaaga agctacacgc ttcaacaaag
     1501 cacggtgcaa tgggtgcaaa acaatgccga gttggaatta aatggcttga gcacagccac
     1561 accaattcag ttttatggtt cgccaacagg gcaacaagca tacatccgca aagactctgg
     1621 tttggtttgt gccgattatg tcaacattcg cgatatgtgg gcaattggaa acggaaacga
     1681 tgcttttgga acgtgttcgc ccaacaatta ctgtactcat accaacccat ggataccatc
     1741 atcgtgtgat acgattgtag atttgttgag ctcttgcggt ccgtggacca tcaaccctgc
     1801 agatcacaac cgtggtagag ctgattttaa tggaggagag tatgccgatt tccaaggagg
     1861 aaatactttt ggttgggata aacgtgccta tccacctgct ccattggtga gtttaaccaa
     1921 tttaaaagat tctatttgta gaatggacag tgttgccatt caagtttctg gagttggtac
     1981 tttgccattt atcattacat ggaaagaaaa tggtgtttac catgccgata ccatttattc
     2041 tgctgcagag ttagattctt acaacccaac aacaagtgaa tttgaaataa taacttggta
     2101 ttacccaatg gcagatattt ctattactga ttttgctata aaaacagaac gttgtcttgg
     2161 aagtgatagc cctgtggata atggagttgc atcaattact atgttgccgt gctatttagg
     2221 tgtagaactg ttggatttta aagctacact taaaaacaca gatgtgctct tgaattggtc
     2281 aacagcaagc gaactcaaca acgattattt tgagttgcaa tatacccacg atggcgtaaa
     2341 ttgggaagta gtggataaag taaaaggcaa aggtacaacc cacaccttga acaattatag
     2401 ctatgttcac tatcgaccat ttggacaaag caacaccttg tattatcgac taaaacaagt
     2461 tgattttgat ggtgcgtttg actattcacc gatacgttct gtagccctca ataatggtga
     2521 agggtatgtg gtatatccta atcctgcaac caatgtgttg tatgtaagtg gaaaaggagc
     2581 ttttaactac caaatgctgg atgcatcagg aaaagtagtt gcagaaggcg caagtagtgg
     2641 tcaagatgaa atttcattgt ttcaattaaa tccaggagtt tatatgttag aaataacaga
     2701 tgatcaagga gtaaaagaaa tatttaagat tgttaagatt taagatttaa aatttaaaat
     2761 aaggttaggt tctcataagt atattaagtg tgagttattt ttaggttatc cacta
//
LOCUS       DAOQVQ010000311         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42221_length_2814_cov_4.015585, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000311 DAOQVQ010000000
VERSION     DAOQVQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42221_length_2814_cov_4.015585"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKN22_08840"
     CDS             <1..403
                     /locus_tag="PKN22_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42851.1"
                     /translation="ADGNLDGNFPTQTGQSNFTVKNITFDLRLAASADSDVSNLTDKM
                     QDVIKVRRGANVSITNALVKGSGTVVDLIDFNDGKGTGDVNAAVSLTNSLTNPFLGKE
                     VNPTTGFPGGKVEAGNSGCSTNNFSWTNYNF"
     gene            529..1224
                     /locus_tag="PKN22_08845"
     CDS             529..1224
                     /locus_tag="PKN22_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS42852.1"
                     /translation="MKGQKILLVDDDQNISEILEFNLKSEGFDVTSVYSAEEALQQPI
                     ASFNLLLLDVMMGGMSGYKLAEKLRNDNITVPIIFLTAKDTENDMLTGFSVGGDDYIS
                     KPFSIKEVIARIKAILKRSNAIETPKQENASNLIIGDLKIDFTLKEIYIANEKIGATK
                     TEFEILSLLAQKSDKIFSRQEIIDILWSDTPYITERTVDVHITRLRKKLKDYASIITN
                     RSGYGYRFNKDVN"
     gene            1276..>2814
                     /locus_tag="PKN22_08850"
     CDS             1276..>2814
                     /locus_tag="PKN22_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNS42853.1"
                     /translation="MFALFSIGVAVLEHYREKEFKTQALEEKLEAYTEVIDTKIDQIN
                     DTTETLRELELLMPSNLRITLIDFKGNVLYDNSIEEFDELKNHSSRPEIVMAKKNKKG
                     TTIRTSDSNQHPYLYFAKKSKNLYIRVALPYDLHLQNFLKANNGFLYYILLFFLLVLF
                     IIHKITTQFGSTVQKLRDFALQPDKSSTNFQFPNDELGEIGKQITNNYMALEESKKSV
                     VLEKQKLLKHIQISEEGICFVSASNQIELYNGLFIQLLNLIVDNSASEAHAIFTADVF
                     RPAIDFINTKTDNYFELTIKKHGKIVSLRIFIFEDNSYEIILNDITQKEKTRQLKQEM
                     TGNIAHELRTPIAGIRAYLETILTQEIPEEKKAHFVQQAYNQSIALSDLIKDMSFLSK
                     IEEAPQAFELETVDMHTLLEKLKAEEQQQLTAKNCTFTWDFPTPFTLRGNYSLIHAIF
                     RNLVENALRYAGENCSIHTALLKEDAKFYYFSFYDTGVGIQDESHLNRIFERFYRVHE
                     GKTHD"
BASE COUNT          980 a          537 c          491 g          806 t
ORIGIN      
        1 agctgatggt aacttagacg gtaatttccc aacacaaact gggcaatcta acttcacagt
       61 aaaaaacatt acgtttgatt tgcgtttggc agcatctgca gacagcgatg tttctaactt
      121 aacagataaa atgcaagatg taattaaagt tagacgtggt gcaaatgttt caattaccaa
      181 tgcgttggta aaaggaagcg gaactgttgt tgacttaatt gatttcaacg atggaaaagg
      241 aactggagat gtaaatgcag ccgtaagttt aaccaatagc ttaacaaacc cattccttgg
      301 taaagaagta aaccctacaa ctggtttccc tggtggaaaa gttgaagcag gaaattcagg
      361 atgttcaacc aataatttct cttggacaaa ttacaatttt taatcatcgc acaaaaattg
      421 agaaattcag aattaaaaga gcgcagaacg aatagtttct gtgttctttt tctcgtttaa
      481 ataaatattc ttattaaaaa ttttaccttt gtttaaaaca atttcagaat gaaaggacaa
      541 aaaattctat tggttgatga cgaccaaaac atttcagaaa ttttagaatt caacctaaaa
      601 agcgaaggct ttgatgtaac aagtgtttac tcagccgaag aagctctaca acaacctatt
      661 gcatcgttta atttattgct cttagatgtc atgatgggag gcatgtcggg ttataaatta
      721 gccgaaaaat tacgcaacga caacattact gtgcccatta tatttttaac ggcaaaagat
      781 acagaaaacg atatgttgac aggtttttcg gttggtggcg acgattacat ctctaaaccg
      841 ttttccatca aagaagtcat tgcccgcatc aaagcaattt taaaacgcag caatgccatt
      901 gaaacaccaa agcaagaaaa cgcaagcaac ttaattattg gtgatttaaa aattgatttt
      961 acactcaaag aaatttatat tgcaaacgaa aaaattggtg caaccaaaac agaatttgaa
     1021 atcctttctt tgttggcgca aaaatcagat aaaatatttt cgcgtcaaga aataatcgat
     1081 attttgtgga gcgatacacc ctatatcacc gaaagaactg tggatgtgca catcacgcgt
     1141 ttgcgtaaaa aattgaaaga ctacgcaagc attatcacca accgttctgg atacggttac
     1201 cgtttcaaca aagacgttaa ttaaacacct atgaagctga gttacagaca acggttgttt
     1261 ctatattttg gggttttgtt tgccttattt tcaattggtg tggctgtttt agaacactac
     1321 cgcgagaaag aatttaaaac acaagccttg gaagaaaaat tggaggccta taccgaggtt
     1381 attgatacta aaattgatca aatcaatgat acaacggaaa cattaaggga attagaattg
     1441 ttaatgcctt ctaatttacg cattaccttg atagatttta aaggcaatgt gctttatgat
     1501 aattcaattg aagaatttga tgaattaaag aatcatagct cccgtccaga aattgtcatg
     1561 gcgaaaaaaa acaaaaaagg aacaaccatt cgcacgtctg actccaacca acatccttat
     1621 ttgtactttg caaaaaaatc aaaaaacctc tacatccgtg tggctttgcc ctacgatttg
     1681 catttgcaaa actttctaaa agccaacaac ggatttttat actacatcct attatttttc
     1741 ttgttggtgc tctttatcat tcacaagatt accacacaat ttggttctac ggttcaaaaa
     1801 ctacgcgatt ttgcgttgca acccgataaa tcatctacca atttccaatt tccaaacgat
     1861 gaattgggcg aaattggcaa gcaaattact aacaactaca tggccttgga agaaagcaaa
     1921 aaaagtgtgg tgttggaaaa acaaaagttg ctcaagcaca ttcaaatctc tgaagaagga
     1981 atttgttttg tttcggcatc caaccaaatt gagttgtata acggtttgtt tatccagttg
     2041 ctcaatttaa ttgtagacaa ttcggcaagc gaggcccacg ccatttttac tgccgatgtt
     2101 ttcagacctg ccattgattt catcaatact aaaaccgaca attattttga gttaaccatc
     2161 aaaaaacacg gcaaaattgt ttcgttgcgc atatttattt ttgaggacaa cagttatgaa
     2221 attattctaa acgacattac ccaaaaagaa aaaacacgcc agttgaaaca agaaatgaca
     2281 ggcaacatag cacacgaatt gcgcacacca attgctggga ttcgcgctta tttggaaacc
     2341 atcctcacgc aagaaattcc cgaagaaaaa aaggcacatt ttgttcaaca agcctacaac
     2401 caatccatcg ctctttctga tttaatcaaa gacatgagtt tcttgtcaaa aattgaagaa
     2461 gcaccgcaag catttgaatt agaaacggtt gatatgcaca ctttgttgga aaaactaaaa
     2521 gccgaagaac agcaacaatt gacagcaaaa aattgcacat ttacctggga cttccctact
     2581 ccattcacgc ttcgtggcaa ctacagcctc atccatgcta ttttcagaaa tttagttgaa
     2641 aatgccctcc gttatgccgg cgagaattgc agcatccaca ctgctttgct aaaagaagat
     2701 gctaaattct attatttctc tttttacgac acgggagttg gcatccaaga tgaaagccat
     2761 ttgaaccgta tttttgaacg tttctaccgt gttcacgaag gcaaaaccca cgac
//
LOCUS       DAOQVQ010000312         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42227_length_2814_cov_3.282349, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000312 DAOQVQ010000000
VERSION     DAOQVQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42227_length_2814_cov_3.282349"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKN22_08855"
     CDS             <1..730
                     /locus_tag="PKN22_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNS42854.1"
                     /translation="AIPSFARKTMAGLMEIIPSEALPVLRNKYNFHNRYEKLKGVLKN
                     PSPTEIMLSLSQQFTDQQIDQLMAFKVQLPSNLYKSEELKKAYFTPLSYMMAIDYQTY
                     LVDDILQKVDRATMSVSLEGREPLLDHRLIEWAATLPDNFKYNKGVKKYILREIAHQY
                     IPEQLLNRPKMGFAIPIAKWLANELRPYVDNYINEERITQQGLFNWQYVSKLKTNFYS
                     GKKELDVKIWYLLMFQMWYEKWME"
     gene            718..1947
                     /locus_tag="PKN22_08860"
     CDS             718..1947
                     /locus_tag="PKN22_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS42855.1"
                     /translation="MDGVKKALYISYDGMTDALGQSQVLPYLVGLSKKGVQITLISCE
                     KPEKLEQFREKIETICQQATIDWHPLKYTKRPPLLSTLWDLWAIKRKAKQLHRTKKFD
                     LVHCRSYIAALAGMDLKKRFGLKMVFDMRGFWADERVDGKIWNISNPVFNKVYRFFKQ
                     KERLFFSESDKIVSLTENGKQEILSWNLPQVSSDKIVVIPCCVDTSLFDPITVEKNKT
                     EELKTQLGIQPSDYIVGYVGSIGTWYMLPEMLDYFNVLKKHQPQARFLFVTNEQPSTI
                     LNLAQEKGISSSDLLITACFHKEMPLYISTFDVSVFFILPSYSKKASSPTKQGEIMAM
                     GIPLVCNAGVGDTDSIVKKYSAGNVVEEFTENQYEKAMNFTTTFNQKEAQKGALEYFS
                     LDNGVARYWEIYQEIIG"
     gene            2003..>2814
                     /locus_tag="PKN22_08865"
     CDS             2003..>2814
                     /locus_tag="PKN22_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNS42856.1"
                     /translation="MNTNGSGRGKSTRTSRANSTASKSKSTTKKSSSTGKSQKPKLTN
                     KEIKAKSRFVNLKDKQKKGDPLPSFNEDAIRLNKYLSNAGICSRREADVLIATGVISV
                     NGKIVTELGYKVAPTDVVKYDEETIRAEVKRYVLLNKPKGFVMVANDPNSKKSADYLV
                     RKACKERIYPVDRLDKETSGLVLFTNDGDLAKKLVHPLRKAAQIFEVELNKPLKKEDV
                     EKLRSGVDLEDGTSRFEEIEFTNPSYFEVGVKIRSTKNQLIRRTFEALGYTVV"
BASE COUNT          949 a          448 c          600 g          817 t
ORIGIN      
        1 tgcgattccg tcgtttgctc gaaaaacaat ggctggtttg atggaaatta ttccttcgga
       61 agcattgcca gtgttgcgca acaaatataa ctttcacaac cgttacgaga agttgaaagg
      121 agttttaaaa aacccttcgc ccaccgaaat tatgttgagt ttgagtcagc aatttaccga
      181 tcaacaaatt gaccaattaa tggcgtttaa ggtgcaattg ccttctaatt tatataaaag
      241 tgaggaattg aaaaaagcgt atttcacgcc actttcgtat atgatggcta ttgattatca
      301 aacctatttg gtggatgata ttttgcaaaa agtggataga gcaacgatgt cggttagttt
      361 agaaggacgt gaaccgttgc tcgatcatcg cttaattgaa tgggcagcaa cacttcccga
      421 caattttaaa tataacaagg gagtaaaaaa atacattttg cgtgaaattg cgcaccaata
      481 cattcctgag cagttattga accgccccaa aatgggattt gccattccga ttgcaaaatg
      541 gttggctaat gaactacgtc cgtatgttga taattacatc aacgaggaac gcattaccca
      601 acaagggttg tttaactggc aatatgtttc caaattgaaa accaatttct atagtggcaa
      661 aaaagaattg gatgtgaaaa tctggtattt gttgatgttt caaatgtggt atgagaaatg
      721 gatggagtaa aaaaagcatt gtatatttct tatgacggca tgaccgatgc actggggcaa
      781 tcgcaggtgt taccctattt agttggtttg agtaaaaagg gggttcaaat caccttgatt
      841 agttgcgaaa aaccagaaaa gttggaacaa tttcgagaaa aaattgaaac catttgtcaa
      901 caagcaacaa ttgattggca tccgttgaaa tacaccaaac gtccgccatt gctctcaact
      961 ctttgggatt tatgggcaat taaacgaaaa gctaaacaac tccaccgtac aaaaaagttt
     1021 gatttagtac attgcagaag ttacattgct gcattggctg gaatggattt aaaaaaacgt
     1081 tttggtttaa aaatggtttt tgacatgcgt ggtttttggg cggatgaacg tgtggatggt
     1141 aaaatttgga atatttctaa tcccgttttt aacaaagttt accgtttttt caagcaaaaa
     1201 gaacgtttgt tttttagcga gtcggataaa attgtgagtt tgaccgaaaa tgggaaacaa
     1261 gaaatacttt cgtggaattt accacaagta tccagcgata aaatagtggt tattccttgc
     1321 tgtgtggata cttctctatt tgacccaata actgtagaaa aaaataaaac agaagaatta
     1381 aaaacacaat tgggtattca accatcggat tacattgtgg gttatgttgg ttcaattggc
     1441 acttggtaca tgttacccga aatgttggat tatttcaacg tattaaaaaa acaccagcca
     1501 caagcgcgtt ttttatttgt taccaatgag cagccttcaa ctattttgaa tttggcgcaa
     1561 gaaaaaggta tttcaagctc cgatttattg attacagcgt gttttcacaa agaaatgccg
     1621 ttgtatattt caacgtttga tgtatcggtg ttttttattt taccatcgta ttctaaaaaa
     1681 gcctcctctc ctaccaaaca gggagaaatt atggcaatgg gtattccgtt ggtgtgcaat
     1741 gctggtgtag gagatacgga tagcattgta aaaaaataca gtgctggaaa tgtggtggaa
     1801 gagtttaccg aaaatcagta tgaaaaggcc atgaatttta cgacaacgtt caaccaaaaa
     1861 gaagctcaaa aaggcgcttt ggaatatttt tctttggaca atggtgtggc gcgttactgg
     1921 gagatttatc aagaaatcat tgggtaaaac cttgctattc aatttttcat tgtaaatttg
     1981 cactttatta attctgctcg agatgaacac aaatggaagc ggtagaggaa aatctaccag
     2041 aacaagtaga gccaattcga cggcttcaaa gtcgaaatca actacaaaaa aaagtagttc
     2101 aacaggcaaa tcacaaaagc ctaaattaac taacaaagaa atcaaggcaa aaagtcgttt
     2161 tgtaaacctt aaagacaaac aaaagaaggg tgatccgctt cctagtttta acgaagatgc
     2221 aatacgcttg aacaaatatt tgtcgaatgc gggtatctgt tcacgaagag aagctgatgt
     2281 attgattgca acaggtgtta tttctgtgaa tggaaaaatt gtaaccgaat tggggtataa
     2341 agtggcgcca accgatgttg ttaaatacga cgaagaaacg atacgcgcag aggttaaacg
     2401 ttatgttttg cttaacaaac caaaaggatt tgtgatggtt gccaatgatc caaacagcaa
     2461 aaaatcggcg gattatttag tgcgaaaagc ttgcaaagag cgcatttatc ctgttgacag
     2521 attggacaaa gaaacatcgg ggttggtttt atttaccaac gatggtgatt tggcaaaaaa
     2581 gttagtgcat cctttgcgca aagctgctca aatttttgag gttgaattga acaaaccgtt
     2641 gaaaaaagag gacgttgaaa aattaagatc gggtgtggat ttggaagacg gcacatcgcg
     2701 atttgaagaa attgaattta ccaatccttc gtattttgaa gtaggtgtaa aaataagaag
     2761 caccaaaaat cagttaatac gcagaacatt tgaagcgttg ggttatactg ttgt
//
LOCUS       DAOQVQ010000313         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42266_length_2812_cov_4.006166, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000313 DAOQVQ010000000
VERSION     DAOQVQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42266_length_2812_cov_4.006166"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN22_08870"
     CDS             complement(<1..874)
                     /locus_tag="PKN22_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS42857.1"
                     /translation="MAKLTLFLLAFFCTSTLVMGQSGAFGFKNLEQSQKERVSTYYLP
                     NQEKYRQGILKNNWKITAENKNWIYFSTTANDLDKAFKSGDIPDYFIKYTVPRALNDT
                     MREHHRVNMVHNGTGLTSAYKGKDVVIGLVDTGIETDHPDFIDANGKTRILRLWDQST
                     DSSSNISPYGYGLIWDSTHINSNYVNGNDNGGHGSTVAGAAAGNGRATGYNQGVAPEA
                     DIIMVKTDFNLPNWHITVAEACDYIFKVADSLGKPAVVNLSVGDYMGSHDGTDPASAY
                     IDSLLDAKNGRIVVA"
     gene            985..2586
                     /locus_tag="PKN22_08875"
     CDS             985..2586
                     /locus_tag="PKN22_08875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNS42858.1"
                     /translation="MRKRIIQLSLAVFAVGIMNVLNAQSVEKDVLNWYNGKSGMQTEK
                     AYKLLKKRMSQPVIVAIVDSGVDVEHEDLKGKIWVNEKEIPGNGIDDDNNGYIDDIHG
                     WNFLGNAKGENQNYARLERTRILARLSKKYDGVEESAVKDADKKEFALYQKVKEEVAN
                     ERAEYEPYVGQMDQLDGMLDQIPQMVSAAIGKENYTEKDLKKWKANDPQSMQLKQLAL
                     AMVTGKLTKEVIQMQKDQINQMLDYNLNVEYDDRALIGDDPYNINDKKYGNADVEGPD
                     ALHGTHVGGIVGAVRGNKIGGDGVCENVKLMALRAVPNGDEFDKDIALAIRYAVDNGA
                     QIINMSFGKGYSPLAKDVYEAFAYADAKGVLLVHAAGNDAKDIDVEPNFPTSNYEFQT
                     KPFDHYLTIGASTRYAKVKKQDFGQLAASFSNYGQKGVDVFAPGFEIYNTIPDNKYMN
                     LQGTSMAAPMVSGAAALMKSYFPNLTMKEIKEILLSTSTNYKGSQQQKPGEKSAVDFG
                     TLSVTGGTINLQAAVKAALAKEKTK"
BASE COUNT          973 a          455 c          562 g          822 t
ORIGIN      
        1 ctgccaccac aatacgtccg tttttggcat ccaacaaact atcaatgtat gcacttgctg
       61 ggtcggttcc gtcgtggctt cccatataat ctccaacact aagattcaca acagcaggtt
      121 tgcccagcga atcggcgact ttaaaaatat aatcacacgc ttcggctaca gtaatgtgcc
      181 aattgggcag gttaaaatct gtttttacca tgatgatatc agcttctgga gctacacctt
      241 ggttgtaacc tgttgcacgt ccatttcctg cagcagcacc agcaaccgta cttccgtgtc
      301 ctccattgtc gttaccgttt acataattgg agttgatgtg cgtgctatcc caaattaatc
      361 cgtaaccata aggagaaatg tttgagcttg aatcggtgga ttgatcccaa aggcgtaaaa
      421 tacgtgtttt accattggca tcaataaagt cgggatgatc ggtttcaatt ccagtatcta
      481 ccaaacctat gacaacatct tttcctttgt aggcagaagt taagccagta ccgttatgca
      541 ccatattgac acggtggtgt tcacgcattg tatcgttgag cgcacgtgga acggtatatt
      601 tgataaaata atcaggaata tcaccacttt tgaatgcttt atccaagtca tttgcggtgg
      661 ttgaaaaata aatccaattt ttattttcag ccgttatttt ccaattgttt ttaagaattc
      721 cttgtctata tttttcttga ttgggtagat aataagttga aacacgttct ttttgacttt
      781 gttccaaatt tttaaaacca aaagcacccg attgacccat aactagggta gaagtgcaaa
      841 aaaaagcgag taagaataaa gttagctttg ccatttatca gtaatttaaa ccaaatatga
      901 gaaaaaaaat gcagtccaac aaaaaaataa cagttaatac attttttatt cgcaaatttg
      961 aagaaattaa tgacattaaa taatatgaga aaaagaatca ttcaattaag tcttgctgtt
     1021 tttgcagttg gaataatgaa cgttttgaac gcacaaagtg ttgaaaaaga tgttcttaat
     1081 tggtataatg gaaaaagtgg tatgcaaaca gaaaaagcat acaaactatt gaaaaaacgt
     1141 atgtctcaac cagttattgt tgctattgtt gactctggtg tggatgttga acacgaagat
     1201 ttgaaaggta aaatctgggt aaatgagaaa gaaatacctg gaaacggaat tgatgacgat
     1261 aacaatggtt acatcgatga tattcacgga tggaactttt tagggaatgc aaaaggtgaa
     1321 aaccaaaact atgctcgttt ggaaagaaca cgtattttgg ctcgcttgtc taaaaaatat
     1381 gatggagtag aagagtctgc tgttaaagat gctgacaaaa aagaatttgc attgtatcaa
     1441 aaagttaagg aagaagttgc aaatgaaaga gcagaatacg aaccatatgt aggtcagatg
     1501 gaccaattgg acggaatgtt agaccaaatt ccacaaatgg ttagcgctgc tatcggtaaa
     1561 gaaaattata ccgaaaaaga cttgaaaaaa tggaaggcaa acgatcctca aagcatgcaa
     1621 ttaaaacaat tggctttggc tatggtaact ggaaaattaa caaaagaagt tatccaaatg
     1681 caaaaagatc aaatcaacca aatgcttgac tacaacttaa atgttgaata cgatgataga
     1741 gcattgattg gtgatgatcc atacaacatt aacgataaaa aatacggaaa tgcagatgtt
     1801 gaaggcccag acgcattgca cggaacacac gttggcggta ttgttggtgc tgttagaggt
     1861 aacaaaattg gtggtgatgg tgtttgtgaa aacgtgaaat taatggcttt aagagcagtt
     1921 cctaatggtg atgagtttga taaagacatc gctttggcaa ttcgctatgc ggtagataat
     1981 ggtgcacaaa tcatcaatat gtcgtttggg aaaggttatt caccattggc aaaagatgtt
     2041 tatgaagcat ttgcttatgc tgacgctaaa ggggtgttgt tagtacatgc tgctggtaat
     2101 gatgcaaaag acattgatgt ggagccaaac ttcccaactt cgaattatga attccaaaca
     2161 aaaccgtttg atcattattt aacaattggt gcttcaacgc gctatgctaa agttaaaaaa
     2221 caagattttg gtcagttggc agcttctttt tctaactacg gacaaaaagg agtggatgtt
     2281 tttgcacctg gatttgaaat ttacaacact attccagaca ataaatacat gaacttgcaa
     2341 ggaacatcaa tggcagcgcc aatggtttct ggagcagcag ctttgatgaa atcttatttc
     2401 cctaatttaa caatgaagga aattaaagaa attttattga gcacttcaac aaattacaaa
     2461 ggttctcaac aacaaaagcc aggagaaaaa tcggctgttg atttcggaac tttatctgta
     2521 acaggtggta caattaactt gcaagcagca gtaaaagcag ctttagctaa agaaaaaaca
     2581 aaataataag ttgtatattt agttttacaa aatgggatgt ctgaaaagat gtcccatttt
     2641 tttattacca atgtctagcc ctgaaatagc gatacacaat tttttatcgt tatggaagaa
     2701 ggagaaaaaa atgtttattt aaaacgtagt caaaaggatt attcaatgtc cattaaatta
     2761 gcagttgtta aagaagttga atcaggattt atttcagtta ctggtgttac aa
//
LOCUS       DAOQVQ010000314         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42315_length_2810_cov_4.055535, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000314 DAOQVQ010000000
VERSION     DAOQVQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42315_length_2810_cov_4.055535"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKN22_08880"
     CDS             complement(<1..605)
                     /locus_tag="PKN22_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS42859.1"
                     /translation="MDKLIIDAKKRSIVPNIKELIRYKDLFWTLSWRDFKVRYAQTTI
                     GLLWALLQPVVTIAILSIVFGRFVGVKTEVPYLLYNVSGMAIWTYFSYVLSNSGNSII
                     AAQQMVKKIYFPRLIIPLSKAVVGLIDLGIVLLILIVLMFYYQVVPSANIWAAPLFIL
                     LAMVASLGAGIWLSALTVRFRDFQHVLPFIVQIGLYVTPIA"
     gene            complement(684..2273)
                     /gene="bshC"
                     /locus_tag="PKN22_08885"
     CDS             complement(684..2273)
                     /gene="bshC"
                     /locus_tag="PKN22_08885"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNS42860.1"
                     /translation="MKVTAIHRKNTGYFSQQQLDMCYNQQHYAKFIAQPFSVAAFEQQ
                     LLVKKNAYSMEMRQQLVQAIKQQYANIALSDKVAHHLDLLAKENTFTVVTGHQLVAMT
                     GPLYFIYKIAHVVRMCTELSQKYTDSQFVPVFWMATEDHDYEEIKSFHLFNRTLTWET
                     TQKGPVGRFDLTGWQEVLDQLKELFASHPQSDVFAWMEQLKGDNYAAAFRRLVNFLFE
                     KYGVVIIDGDDAQLKQAFVLIMQKEVLEQFAFPAIRATTERLVVHGGKQQVMPREYNL
                     FYIENGMRERLIPKGDAIEIPQKGIFSKQEVAEWIARSPQNFSPNASMRPLYQEVLLP
                     NLCYIGGAGEINYWLQLKGVFDAVDVPFPLLKTRNSLLWIDAATQGKMEQLGWTTDDL
                     FKETHQLTAAYLEKNAGDAIDFSPIQAHVEVLKNTVIETFHSAEPSMDSYVMAELVKL
                     DKQMKQLEDQLVKASKRQHEKALKTIKQVKERLFPAGGLQERTTNFLQLAPDGNFKPL
                     IDAIVASIQPFSDDFLVVEEK"
     gene            2394..>2810
                     /locus_tag="PKN22_08890"
     CDS             2394..>2810
                     /locus_tag="PKN22_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42861.1"
                     /translation="MAETIKYSKIHAFSEHAAAVYTLQFDGKFLYSGSADKYVVRWDV
                     EQKQQDKFAIQLPLTPYCIQLFDNNTKLAVGLSNGDVHLFDLDNRKEIKYIRLHKAGV
                     FSMAENKAKRHLYISDADGLLSVWDIDTLTLLLQLPF"
BASE COUNT          887 a          600 c          484 g          839 t
ORIGIN      
        1 taggcaatcg gggtaacata caagccaatc tgaacaataa acggcaatac gtgctgaaaa
       61 tcacgaaaac gaacggtcaa tgcactcaac caaatacccg ctcccaacga tgccaccatt
      121 gccaacaaaa taaacaatgg agctgcccat atatttgccg atggaacaac ttggtagtaa
      181 aacatcaaaa cgattaaaat caacaacact atgcccaaat caattaagcc aacaacggct
      241 ttggacaaag gaataatcaa acgtggaaaa tagatttttt tgaccatttg ctgtgccgca
      301 atgatggaat ttccagaatt ggacaacaca tacgaaaaat acgtccaaat ggccatcccc
      361 gaaacgttgt atagcaaata tgggacttcg gtttttacac ctacaaaacg accaaaaaca
      421 atggacaata tggcaatcgt tacaactggt tgcaacaaag cccacaacaa accaatggtg
      481 gtttgcgcat aacgtacttt aaaatcgcgc caagacaatg tccaaaacaa atctttgtaa
      541 cgaatcaatt ctttgatatt gggaacaata gaacgttttt ttgcgtcaat gattaattta
      601 tccatctttt ggttttattc ataagtcttt ctgggcaaca aacatacaca ttccttttga
      661 attgattgaa acagatgaag tgcttatttt tcttctacca ccaaaaaatc atccgaaaat
      721 ggttgtatgc tggcaacaat agcatcaatt aagggcttga aattaccatc aggtgccaat
      781 tgcaaaaaat tggttgttct ttcttgcaac ccacctgctg gaaaaagacg ttctttcacc
      841 tgtttgatgg ttttcaatgc tttttcgtgt tgacgctttg aagctttaac caattgatct
      901 tccagctgtt tcatttgttt atccaatttc acaagttctg ccataacata cgaatccatc
      961 gatggttctg ccgaatgaaa tgtttcaata accgtgtttt tcaacacctc aacgtgtgct
     1021 tgaatgggtg aaaaatcaat ggcatcgcct gcattttttt cgagataagc tgccgtaagt
     1081 tggtgggttt ctttgaataa atcatcggtg gtccaaccca attgctccat ttttccttgt
     1141 gttgcagcat ctatccacaa caacgaattt cttgttttta gcaatggaaa cggaacatca
     1201 acagcatcaa acacaccttt taattgcaac caataattga tttcgcccgc tccaccaata
     1261 taacacaaat tgggcaacaa tacttcttga tacaaaggtc gcatagacgc attgggcgaa
     1321 aaattttggg gagaacgtgc aatccattca gccacttctt gttttgaaaa aattcctttt
     1381 tgcggaattt caatagcatc tcctttcgga attaatcgct cacgcatgcc gttttcaatg
     1441 taaaacaagt tgtattcgcg cggcattact tgctgttttc caccgtgcac caccaaacgt
     1501 tcggtagttg cccgaatagc aggaaacgca aattgctcca acacttcttt ttgcattatc
     1561 agcacaaaag cttgtttcaa ttgcgcatca tcgccatcaa ttataacaac accgtatttt
     1621 tcaaataaaa aattcaccaa tcgacggaaa gcagcggcat agttatcgcc ttttaactgt
     1681 tccatccatg caaaaacatc actttgcgga tgactggcaa acaattcttt caattgatcc
     1741 agcacttctt gccaacctgt caagtcaaat cgaccaacgg ggcctttttg cgttgtttcc
     1801 caagtgagtg tgcgattaaa caaatgaaac gattttattt cttcgtaatc gtgatcttcg
     1861 gttgccatcc aaaatacagg tacaaactgc gaatcggtgt atttttgcga caattcggta
     1921 cacatacgca caacatgcgc aattttgtaa ataaaataca acggacccgt cattgccacc
     1981 aattgatgtc ctgttacaac tgtaaacgtg ttttcttttg ccaacaaatc aaggtggtgc
     2041 gccactttat cagatagcgc aatgttggca tattgttgtt tgatggcttg caccaattgt
     2101 tgtcgcattt ccatcgaata cgcgttcttt ttcaccaaca gttgttgttc aaaagcagca
     2161 acagaaaacg gttgtgcaat aaacttggca tagtgttgct gattgtagca catgtccaat
     2221 tgttgttgag aaaaatatcc cgtatttttt cgatgaatgg ctgtaacttt catggtgcaa
     2281 atttaatgat tttactgcac aattaattcc ttgttcaaaa tactatttag aataattcta
     2341 aataaattgc aatttacaac aaaaaaatac taactttggc aacaaattaa gcaatggcag
     2401 aaacaatcaa atattctaaa attcacgctt tctcagaaca tgcagcagca gtttacaccc
     2461 ttcaatttga tgggaaattc ctttattcgg gtagtgcaga taaatacgtt gttcgttggg
     2521 atgtggagca aaaacagcaa gataaatttg ccattcaatt accgcttacc ccctattgca
     2581 ttcagctgtt tgacaacaac acaaaattgg ctgttggttt atccaatggc gatgttcatt
     2641 tgtttgattt agataaccga aaagaaatta aatacattcg tttgcacaaa gcgggagttt
     2701 tttcaatggc cgaaaacaaa gcaaaacgac atttatacat ttccgatgca gatggattgc
     2761 tttctgtttg ggatatagac acgctcaccc tccttttaca acttccattt
//
LOCUS       DAOQVQ010000315         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42366_length_2808_cov_3.498002, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000315 DAOQVQ010000000
VERSION     DAOQVQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42366_length_2808_cov_3.498002"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKN22_08895"
     CDS             complement(<1..717)
                     /locus_tag="PKN22_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42862.1"
                     /translation="MNRRNFLAVFVAFLCWQLSFSQVDDVKRIVKQLCSPEFHGRGYV
                     NGGDSIAAEFIAREFQTIGLKPVGKSYFQAFHFPVNSFPKNAQFTLVKKDGSTEELEV
                     GRTIIAKENSPSYRGTLHYRIVPTHLLFQKDSLQAIIQTAMQGKTYNAIALDFRTTAA
                     DTLKLIRGAEEQFATYVPVILLTDKKFTWSVASTATNFPILELQATKADGSPIRVELD
                     AVLKQHHARNVVGFLPAKKKT"
     gene            complement(710..1522)
                     /locus_tag="PKN22_08900"
     CDS             complement(710..1522)
                     /locus_tag="PKN22_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42863.1"
                     /translation="MAKYLSQKELVEKISENISKMETAQMDLQGIEAHLDVVRELYER
                     TIALRYKAFEAKASVAVTPSVKEEVAPMVAAQEPEIVAANTFEEPTPIIDETPKAAVL
                     DFDIFDAPSTVEEEISFSTEEEGAIIDEIVEEVAVFEPVEQAPVVEEQPNIPTPIVNT
                     PIEAPVTAPSMSSDFVSLTQTINNKVKNHIGFMPLDSLVGSFGLNERLLFINELFDGS
                     SEAFSDAIKHLDARHSLSEAAHYLNELGSQYAWETDNETLEEFIQKLCRRFE"
     gene            1732..2394
                     /locus_tag="PKN22_08905"
     CDS             1732..2394
                     /locus_tag="PKN22_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42864.1"
                     /translation="MKKVVIVALLSFLLINAESVNAQAFGGKGSKQISISLGMANHNT
                     WFSYNNHGVSGRFSPLSGVVSVQGEFGIHKYVGIGFDAGFNFSNSLWGFYGAKYGPYY
                     HDYDDDWDGYYGWGSAYRGFGIPISFIANFHFLQLIADKTGKSFAEKLDVYGGLSLGS
                     GPFFAILKNGYKNYNGNDIGAMLIVGPHVGIRYYPKGNVGIHAEVGYGKSIINGGVTF
                     KM"
     gene            2473..>2808
                     /locus_tag="PKN22_08910"
     CDS             2473..>2808
                     /locus_tag="PKN22_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNS42865.1"
                     /translation="MTNSSESHYFYHMFLEQFPGNQKKNGWIEVICGSMFSGKTEELI
                     RRLRRAEFANQQLLLFKPKIDNRYHNKKVVSHKGSDFDAEIVEHSSEILNHWNGELVV
                     AIDEAQFFDN"
BASE COUNT          879 a          473 c          525 g          931 t
ORIGIN      
        1 agtttttttc ttagcgggca aaaacccaac cacattacgc gcatggtgtt gttttaaaac
       61 ggcatccaat tcaacacgaa taggggagcc atctgctttt gttgcttgta gctctaaaat
      121 tggaaaatta gttgcagttg aagctaccga ccaagtaaat tttttatccg ttagcaatat
      181 aacaggcacg taggtagcaa attgttcttc cgctccgcga attaatttta atgtgtctgc
      241 tgccgtagtt ctaaaatcca acgcaatggc attgtatgtt tttccttgca tggccgtttg
      301 aataatggct tgcaaactgt ctttttgaaa caataaatgg gttggaacaa tacgataatg
      361 gagcgttcca cgatacgatg gtgaattttc tttagcaatg attgttcttc caacctctaa
      421 ttcttcggtt gaaccgtctt tttttaccaa agtaaactga gcgttttttg ggaacgagtt
      481 tactggaaaa tgaaacgctt gaaaatacga tttcccaaca ggttttaatc caattgtttg
      541 aaattcacga gcaataaatt cagcagcaat agaatcacca ccatttacat atccgcgacc
      601 atgaaattcg ggtgagcata attgtttaac tattcgtttt acatcatcta cttgagaaaa
      661 cgatagttgc caacacaaaa atgctacaaa aacagctaaa aagtttcttc tattcaaaac
      721 ggcgacacaa tttttggata aattcttcta aggtttcatt atctgtttcc caagcatatt
      781 gacttcctaa ttcgtttaaa tagtgtgctg cttctgataa gctatggcga gcatccaaat
      841 gtttaattgc atccgaaaaa gcttcgcttg agccatcaaa caattcgtta ataaacaaca
      901 aacgctcgtt taacccaaaa gaaccaacta acgaatctaa aggcataaat ccaatatgat
      961 tttttacctt attattgatg gtttgtgtta atgaaacaaa atcagacgac atagatgggg
     1021 cagtaactgg tgcctcaatt ggggtgttaa caataggagt tggaatattt ggttgttctt
     1081 caactactgg tgcttgctca acaggttcaa atactgcaac ctcttccact atttcatcaa
     1141 taattgcacc ctcctcttct gtagaaaaac taatttcttc ttccacagtt gatggtgcat
     1201 caaaaatatc aaaatctaac acggcagctt ttggagtttc atcaataatt ggagtaggct
     1261 cttcaaacgt attcgccgca actatttctg gctcttgagc agcaaccatt ggggcaactt
     1321 cttcttttac agagggagta acagcaacag acgctttagc ttcaaaagct ttgtatcgca
     1381 aggcaatggt gcgttcgtaa agttctctca caacatccaa atgggcttca attccttgca
     1441 aatccatttg tgctgtttcc attttagaga tgttctcact aattttttct acaagttctt
     1501 tttgagaaag atattttgcc atgactttta tattttgatt gtgttttttt tcaaaattaa
     1561 cgagatattc ttccaacaaa ttgcagacca taaaaatatt ttgaggaaga attgttaata
     1621 actacaatat atcttgtttt ataccgtttc atgaaaagtt aaaaaaaaac gacttggtat
     1681 gcaaattgtt aaaaaaccac gttgttttac aatattaata aattttataa aatgaaaaaa
     1741 gtagtaattg ttgcattgtt atcattcttg ttaatcaatg cggaaagtgt aaatgcgcaa
     1801 gcatttgggg ggaaaggaag caaacaaatt tctattagtt taggcatggc caaccataat
     1861 acatggttct catacaacaa tcatggtgta tcagggaggt tctctccatt atctggtgtt
     1921 gtatcagttc aaggtgagtt tggtatccat aaatatgtag gaattggttt tgatgccggt
     1981 tttaattttt ccaatagttt gtggggattt tatggggcta aatatggacc ttattatcat
     2041 gattatgatg atgattggga tggttattat ggatggggaa gtgcctaccg tggatttggt
     2101 attccaatta gctttattgc caacttccat tttttacagt taattgccga taaaacagga
     2161 aaatcatttg ccgaaaaatt agatgtatat ggtggtttat ctcttgggtc aggaccattt
     2221 tttgccattt tgaaaaacgg atataaaaat tataatggca acgatatagg agctatgttg
     2281 attgttggac cacatgttgg aattcgctac tatcctaaag gaaacgtagg aattcatgca
     2341 gaagttggtt atggcaaatc aattattaat ggaggtgtaa cttttaaaat gtaaacaccc
     2401 tttttttcga cgaaagaaag tgatcattgc aaagtggtca ctttttttgt tgataaaagt
     2461 tttgattttt agttgacaaa ttcatcggaa tcacactatt tttatcacat gtttttagag
     2521 caatttccag gcaatcaaaa aaagaacgga tggatagagg tcatctgtgg ttccatgttt
     2581 tcaggtaaaa cagaagaatt aattcgccgt ttgcgcagag ccgagtttgc caatcaacaa
     2641 ttgttgttgt ttaaaccaaa aattgacaat cgttaccata ataaaaaagt ggttagccac
     2701 aaaggaagtg attttgatgc cgagattgta gaacactcca gtgaaatttt aaaccattgg
     2761 aatggagaat tggtggttgc cattgacgaa gctcaatttt ttgataat
//
LOCUS       DAOQVQ010000316         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42489_length_2803_cov_3.094250, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000316 DAOQVQ010000000
VERSION     DAOQVQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42489_length_2803_cov_3.094250"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PKN22_08915"
     CDS             <1..1150
                     /locus_tag="PKN22_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42866.1"
                     /translation="YNGTTWVLSGHVEYNRLNFVSSELGTVDTGALPANPVSFNTSLT
                     TSYVVGDTLTEEFSNGYINWEFNGATWDLMYAREYSTGVDTNTITSIGVVASFDTSAI
                     PTAPTVAPSSPTTNDVYIETFNNTTRNQAATIRWTYNGTAWVINGVVELKLKAFFATV
                     ASNLTAGALPSTPATPGSSTGYLTGDTLHETYNNGTLDWTYGTSSWSLSASKIATNNT
                     YVIKASTSWEEDGVLPASPENTPSTPIISDILIQSYNNVTRDNAVTRYWSWNGSAWVE
                     LSNGIHRFNMVFTNVPAVDLTYGVAPTASLAATANMYVNGDILWEKYTNGYGMYSFDD
                     GAWVLLTYGLEVGRIQDSADFLATLGPGVDGWAVIYDDTLGGFTLADI"
     gene            1226..1672
                     /locus_tag="PKN22_08920"
     CDS             1226..1672
                     /locus_tag="PKN22_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42867.1"
                     /translation="MTREIRDDDCLKKLNSEEVPIWARSLSLVLTALVGFILALGLNL
                     GDIVNRYMDSRSNIETTQLTQTGQVEVNALTGLIDANKGMTQHITDLILSLNTAIQQN
                     NLLSNANNNLTTENFTLKTTIAQLEDKIKSLESENQDLKTRLELRE"
     gene            1674..1937
                     /locus_tag="PKN22_08925"
     CDS             1674..1937
                     /locus_tag="PKN22_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42868.1"
                     /translation="MCVPCKNKKAAAQAKAVLFNKTRTISEIEFLNLYYVGPEDEEVP
                     TLLNKVTYGKKPYGVQMFVARVDYEAHPELWQENEPSNSNIID"
     gene            1954..2271
                     /locus_tag="PKN22_08930"
     CDS             1954..2271
                     /locus_tag="PKN22_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HNS42869.1"
                     /translation="MCSLFQDELGPFNIFKKIRTFVGLTSVEDLPLNEQILYSDKEFV
                     HNGEFFAELIECIWCLSIWVGGLFSTYLAIVGVIPYSYIPIYTLCSSALTILIKNKEI
                     FNG"
     gene            2264..>2803
                     /locus_tag="PKN22_08935"
     CDS             2264..>2803
                     /locus_tag="PKN22_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42870.1"
                     /translation="MASIMINPVKTGEGRIFINPYNYGGASKFNFHNFMKIEGLDRTL
                     GDIEPIYLPDPNRYDEFITNGYIKGSDSRWSSSLSSKLPIDTQSVLETMANTGCEFTL
                     QVHYGRCTKPDSFQDFESAIILKGVKLTSYGLSPLTALNPGERALVDETASITASEMY
                     RVFSQKFITMTPQTSGRVLR"
BASE COUNT          973 a          392 c          530 g          908 t
ORIGIN      
        1 gtataatgga acaacttggg tattaagtgg tcatgttgaa tataatagat taaactttgt
       61 ttcatctgag ttaggaactg ttgacacagg agccttacct gctaatcctg ttagctttaa
      121 cactagttta actactagtt atgttgttgg cgatacatta actgaagagt tttcaaatgg
      181 ttatatcaat tgggaattta atggtgcaac atgggactta atgtacgcaa gggagtattc
      241 aacaggggtt gatactaata caattacttc aattggcgtt gttgcttctt ttgatacgtc
      301 agcaattcca actgctccaa ctgttgctcc aagttcacct acaactaacg atgtttatat
      361 cgaaacattt aataatacaa cacgcaatca agccgcaaca attcgttgga catacaatgg
      421 aacggcatgg gtaattaatg gtgttgttga attaaaatta aaagcatttt ttgcaactgt
      481 tgcaagcaac ttaactgctg gtgctttacc tagcactcct gcaactcctg gttcttcaac
      541 tggatattta acaggagata cattacatga aacttacaat aatggaacac ttgattggac
      601 atacggtaca agcagttggt cattaagtgc aagtaagatt gcaacaaata atacttatgt
      661 aataaaagca tctacaagtt gggaagagga tggagtttta cctgcttctc ctgaaaatac
      721 accttctaca ccaattatca gtgatatttt aattcaaagc tataataatg tcacaagaga
      781 taatgctgtt acaaggtact ggtcatggaa tggttcagca tgggttgaac tatcgaatgg
      841 cattcaccgc tttaatatgg tatttacaaa tgtaccagca gtagacttaa cttatggcgt
      901 tgctcctaca gcaagtttag cggcaacggc aaatatgtat gtaaatggcg atatattatg
      961 ggaaaaatat acaaatgggt atggaatgta ctcgtttgat gatggtgcgt gggtactcct
     1021 aacctacgga ttggaggtgg gcagaattca agattctgcc gactttctcg ctactttagg
     1081 accaggtgta gatggatggg ctgttattta tgatgatact cttggtggtt ttactttagc
     1141 agatatttaa gccattcctg actactttac atatatatta atatttgata ttagttaata
     1201 tattaaaagg atgtttggag gctaaatgac tagggaaata agagatgatg attgtttaaa
     1261 aaaattaaat tctgaagaag taccaatttg ggcaaggtca ctatcattag tgttaactgc
     1321 tcttgttggg tttattttag ctttaggatt aaatctaggc gatattgtta atcgttatat
     1381 ggactcaaga agtaacattg agacaacaca attaacacag actggtcaag ttgaagttaa
     1441 tgcattaaca ggtttaatcg atgcgaataa aggtatgacg cagcatataa cagatttaat
     1501 tttatcttta aatacagcaa ttcaacaaaa caatttatta tctaatgcta ataataattt
     1561 aacaacagaa aattttactt taaaaacaac tattgcgcaa ttagaagata agattaagag
     1621 tcttgaaagt gaaaatcaag atttaaaaac tagattggaa ttaagagaat aatatgtgtg
     1681 taccatgtaa aaataaaaag gctgctgcgc aagcaaaggc tgttttattc aataaaacta
     1741 gaactattag tgaaattgaa tttttaaatt tatattatgt aggaccagaa gatgaagaag
     1801 taccaactct attaaataaa gttacttatg gtaaaaaacc atatggtgta caaatgtttg
     1861 tagctagagt agattacgaa gctcatcctg aattatggca agaaaatgaa ccatctaatt
     1921 ctaatattat tgactagtat aagcgtatgg cgagtgtgtt cactatttca agatgaatta
     1981 ggaccattta atatattcaa aaaaataaga acttttgtag ggttaactag tgttgaagac
     2041 ttgccattaa atgaacaaat attatactct gataaagaat ttgttcacaa tggagaattt
     2101 tttgcagaat taattgaatg tatttggtgc ttaagtatat gggtaggtgg attattttct
     2161 acttatttag ctatagtagg agttattcct tattcatata ttcctattta tacattatgt
     2221 agttctgcac taacgatatt gataaaaaat aaggagattt ttaatggcta gtattatgat
     2281 taatcctgtt aaaacaggtg agggtagaat ttttataaat ccatacaatt atggtggagc
     2341 aagtaagttc aatttccata atttcatgaa aattgaagga ttggatagaa cacttggaga
     2401 tattgaacca atttatcttc cagatccaaa tagatatgat gagtttataa caaatggata
     2461 tatcaaaggt agtgattcaa gatggagttc aagtttaagc agcaaacttc caattgatac
     2521 ccaaagtgtt cttgaaacaa tggcaaatac aggatgtgaa tttacattgc aagttcatta
     2581 cggacgttgt acaaaacctg atagtttcca agattttgaa agtgctatta ttttaaaagg
     2641 tgttaagtta acctcatatg gactaagtcc attaactgct ttaaatcctg gtgaaagagc
     2701 tttggtcgat gaaactgcaa gtattactgc ttcagaaatg tatagagtat ttagccaaaa
     2761 atttatcaca atgacgcctc aaacttcagg tagagttcta aga
//
LOCUS       DAOQVQ010000317         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42530_length_2801_cov_4.136562, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000317 DAOQVQ010000000
VERSION     DAOQVQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42530_length_2801_cov_4.136562"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKN22_08940"
     CDS             <1..723
                     /locus_tag="PKN22_08940"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42871.1"
                     /translation="HHVEIKWNAATQTLEVYFDGALRLTCNGDFVNTVFGGNNIQYWG
                     ATGATGALNNQQYFCPNTVVILGSTLESFDVKCQNNNLVANWQTSKEKNVDRYEIEYT
                     HDGYVYYTYASVLPTGNDASTTQRYSLPIVGLDPQNTYFRIKTVDRDNVYKVSETVVA
                     QNCQLEETLSIAYAASQLQISSKTMQDFSFNCYDLNGKSIVQGNSMDGVVNKNVSLIS
                     GVYFVTINYTDGKQVNKKIVIN"
     gene            877..2562
                     /gene="ettA"
                     /locus_tag="PKN22_08945"
     CDS             877..2562
                     /gene="ettA"
                     /locus_tag="PKN22_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNS42872.1"
                     /translation="MSDDKKIIFSMVGVSKSTPQGKQIIKDIYLSFYYGAKIGIIGLN
                     GSGKSTIMKIIAGIEKSYQGDVVFSPGYTVGYLPQEPELDPKKTVKEIVMEGVQETVD
                     VLKEYEEINNSFMDEEVLNDPDKMDKLIARQAAVQDKIDALDAWELDSKLEIAMDALR
                     CPPDDQLIETLSGGERRRVALCRLLLQTPDILLLDEPTNHLDAESIEWLEQHLQQYKG
                     TVIAVTHDRYFLDNAAGWILELDRGEGIPWKGNYTSWLEQKGKRLAEEEKSESKRQKV
                     LARELEWVRMNPKGRHAKNKARLANYDKMLAEDAKDKEAKLEIPIPNGPRLGNNVIDA
                     VNVSKAFGDKLLYDDLNFKLPPAGIVGIIGPNGAGKTTIFKMIMGELAPDAGSFEVGD
                     TVKIGYVDQTHKDIHPDKTVYEVVSNGTELIEIGNQKVNARAYLSKFNFNGSDQNKKV
                     GTLSGGERNRLHLAMTLKTEANVLLLDEPTNDIDVNTLRALEEGIENFAGCAVVISHD
                     RWFLDRICTHILAFEGDSHVYWFEGSYSEYEENRKKRLGDTGPKRVKYRKLIK"
     gene            2578..>2801
                     /locus_tag="PKN22_08950"
     CDS             2578..>2801
                     /locus_tag="PKN22_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNS42873.1"
                     /translation="MFATILIDWYQLHKRDLPWRNTSDPYKIWLSEIMLQQTRVDQGL
                     SYFLKFVDHYPTIVDLANATEQQVLNDWQG"
BASE COUNT          940 a          479 c          596 g          786 t
ORIGIN      
        1 caccacgttg aaattaaatg gaatgcagct actcaaactc ttgaagttta ttttgatggt
       61 gctttgcgat taacgtgtaa tggtgatttt gttaacactg tttttggtgg caacaacatt
      121 caatattggg gagcaacagg agcaacaggt gcgttgaata accagcaata tttttgccca
      181 aatacggtgg taatcttagg ttctacatta gaaagttttg atgtaaaatg ccaaaacaac
      241 aatttggtag ccaattggca aacgagtaaa gaaaaaaatg ttgaccgata cgaaattgaa
      301 tatacccacg atggttatgt atattacacc tatgcaagtg tgttgccaac tggaaatgat
      361 gcttcaacaa cacaacgata ttctttgcct attgttggtt tagatccgca aaacacttat
      421 tttagaatca aaacagtgga tagagacaat gtgtataaag tttcggaaac ggttgtggca
      481 caaaattgtc aattggaaga aacactttcc attgcttatg ctgcatctca attgcaaatt
      541 tcctctaaaa cgatgcaaga tttttcgttc aattgctacg acctaaatgg gaaatcaatt
      601 gtgcaaggaa attcaatgga tggcgtagta aacaaaaatg tttctttgat atcgggagtt
      661 tattttgtta ccatcaacta caccgatgga aaacaagtaa acaaaaaaat tgtaattaat
      721 tagtataaat atagtagtaa taggttaggt attagtaatt aagttaggta aacagataat
      781 gagatgattt taattaattg tctcattttt tgtgttcaat cggttcgggc gaaaaccaaa
      841 tattttcatt acttttacac ttcaaatcaa acaaacatgt cagacgataa aaagattatt
      901 ttttcaatgg ttggggtgtc caaatcaacg cctcaaggaa aacaaattat aaaggatatt
      961 tacctttcat tctactacgg tgccaaaatt ggtatcatcg gtttgaatgg ttcgggtaaa
     1021 tcaaccatca tgaaaattat tgctggtatt gaaaaatcct accaagggga tgttgtgttt
     1081 tcaccaggat ataccgttgg atatttaccg caagaaccag agttagaccc taaaaaaacg
     1141 gtaaaagaaa tcgtgatgga aggggtgcaa gaaactgttg atgtattgaa agaatacgag
     1201 gagatcaaca attcatttat ggacgaggaa gtgttaaacg acccggataa aatggataag
     1261 ttaattgcgc gtcaggccgc tgtacaagat aaaattgatg cgttggatgc atgggaattg
     1321 gattctaaat tggaaatcgc catggatgcc ttacgttgtc cacctgatga tcaattaatt
     1381 gaaacgctat ctggaggtga acgtcgccga gttgcacttt gccgtttgtt gcttcaaaca
     1441 cccgatattt tattgctgga tgagcctacc aaccacttgg atgcagaatc tattgaatgg
     1501 ttggaacaac acttgcaaca atacaaagga acggtaatcg ctgtaacgca cgaccgttat
     1561 ttccttgaca atgcagctgg ttggattctt gagttagacc gtggagaagg tattccgtgg
     1621 aaaggaaact acacttcttg gttggagcaa aaaggaaaac gcttggccga agaagaaaaa
     1681 tcagaatcaa aacgtcaaaa agtgttagcg cgcgagttgg agtgggtgcg tatgaaccca
     1741 aaaggtcgcc acgccaaaaa caaagctcgt ttagccaatt atgataaaat gttggcagaa
     1801 gatgcaaaag acaaagaagc taaattggaa attccaattc caaatggtcc gcgtttgggt
     1861 aacaatgtta tcgatgctgt caatgtttct aaagcatttg gcgataaatt attgtacgat
     1921 gatttgaact ttaaactacc cccagcagga attgtaggca tcattggacc aaatggtgct
     1981 ggtaaaacta ccatttttaa aatgatcatg ggtgagttag ccccagatgc tggatcgttt
     2041 gaagtaggcg acactgtaaa aattggttat gttgaccaaa cacacaaaga tattcacccc
     2101 gataaaacgg tgtatgaagt ggtgtctaac ggaacagaat taattgaaat tggtaaccaa
     2161 aaagtaaatg ctcgtgcata tctttctaaa tttaatttca acggatctga ccaaaacaaa
     2221 aaagtaggaa cactttccgg tggtgagaga aaccgtttgc acttagcaat gacactcaaa
     2281 acagaagcca acgtgttgtt acttgacgag ccaaccaacg atatcgatgt aaacacattg
     2341 cgcgccttgg aagaaggtat tgaaaacttt gcaggatgcg ccgtagttat ttcgcacgac
     2401 cgttggttct tggatagaat ttgtacacac atcttagcat ttgaaggcga ttcacacgtt
     2461 tattggttcg aaggttctta ctccgaatac gaagaaaacc gcaaaaaacg ccttggtgat
     2521 acaggtccaa aacgtgttaa gtacagaaaa ttgataaaat agtaagcaga caagcacatg
     2581 tttgctacta ttttaattga ttggtatcaa ctccataaac gggatttgcc gtggcgcaat
     2641 acatccgacc cctataaaat atggctgtcg gaaatcatgt tgcaacaaac acgggttgac
     2701 caagggttga gttattttct aaaatttgtg gaccattatc ccaccattgt tgatttggca
     2761 aatgctacgg aacaacaggt gctcaacgat tggcaaggtt t
//
LOCUS       DAOQVQ010000318         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42936_length_2784_cov_2.578967, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000318 DAOQVQ010000000
VERSION     DAOQVQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42936_length_2784_cov_2.578967"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1622)
                     /gene="lnt"
                     /locus_tag="PKN22_08955"
     CDS             complement(12..1622)
                     /gene="lnt"
                     /locus_tag="PKN22_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNS42874.1"
                     /translation="MRMTRSWRYLLSVLSGVLLTLSFPYTGSLTPLIFIALIPLLLVE
                     HQIAKQRYKSSKVFIHAYISFFIYNIGTTWWIWNADLNGAIMAFVLNSLLMAIAFQIY
                     HFIKKHVGANYGLATLVSVWLGFEYLHFNWELSWPWLTLGNVFSITPSWVQWYEFTGV
                     LGGSLWVLLVNYLLFKALSTYFFSETKTTLQPKKIALPVVILLLPVSTSLWMYSLHSD
                     EGTAAEIVVVQPNIDPYYKFTTITPDQQLHRIADLADKTITTNTKLVIAPETAIPISF
                     DEALIGYDIGYQILQKRMKDWGSLPLFLGASTEKRFDTKVSRAAKKDPYGGDGFVEYY
                     NSSLLMGNDLQPQIVHKSKLVLGAEKVPFSHWFPFLEELSLDFGGTSGTLGVEAQPTI
                     YKNGGFPFTPSICYESIYGEYTAQQTKLGAKAIFVITNDGWWGDTPGYKQHFSFARLR
                     AIENRKSVARSANTGTSGFINQRGDVIQASDWWTETALRNTIFLNDRSTLYMTLGDIL
                     GKLGLVSALVFFLYAFFRKVKPLNVKEV"
     gene            complement(1670..2242)
                     /locus_tag="PKN22_08960"
     CDS             complement(1670..2242)
                     /locus_tag="PKN22_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNS42875.1"
                     /translation="MDFSFKNNLRPKKGALLLSDPFEMGAFFTRSVVLICDYNADGTF
                     GFVLNNYLELTPSEISQELDGYEGRVSVGGPVDSSSLFYIHQLENIEGSEKVMDNLYF
                     GGDFLEIINAIRTQPDNASKVRFFAGYSGWSGGQLEEELEQHAWIVVDNHTIQHIMDT
                     NNDNLWKDIMKLQGSKYKVLSDFPLNPTHN"
     gene            complement(2310..>2784)
                     /locus_tag="PKN22_08965"
     CDS             complement(2310..>2784)
                     /locus_tag="PKN22_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNS42876.1"
                     /translation="NEIEPCMYMGAEKREDGSLISYLDFDPQESIEQSVFSIKQDVKL
                     LGANIVKLGFKKLKGLMDDKQISSSEIDYFLPHMSSFFFEDKMYNEMVELGIEIPKEK
                     WFTNLATKGNVGAASIYLMLDELFNGGQLKKGQKILLGVPESSRFSYVFCLLTVC"
BASE COUNT          942 a          555 c          424 g          863 t
ORIGIN      
        1 aatcattaca actatacttc cttaacattc aatggcttaa cctttctgaa aaatgcatat
       61 aagaaaaaaa caagggcgct tactaatccc aatttaccta aaatatcacc caaggtcata
      121 tacaatgtag agcgatcgtt tagaaatata gtatttctta gcgctgtttc tgtccaccaa
      181 tcagacgctt gtattacatc tccccgctgg ttgataaatc cgcttgttcc agtgtttgca
      241 gaacgtgcaa cagattttct attttcaata gctcttaaac gagcaaagct aaaatgctgt
      301 ttataccctg gagtgtcgcc ccaccaacca tcattggtaa tgacaaaaat tgcttttgcc
      361 cccaattttg tttgttgtgc cgtatattct ccataaattg attcataaca aatacttggt
      421 gtgaagggga aacctccatt tttataaata gttggttgcg cttctactcc caaagttcca
      481 cttgttcctc caaaatcgag tgaaagttct tctaaaaacg gaaaccaatg tgagaaaggc
      541 actttttctg ctcctaatac caatttagat ttgtgtacaa tctgtggttg taaatcattt
      601 cccattagta atgaagagtt gtaatattca acaaatccat caccaccata aggatctttt
      661 tttgctgcac gcgatacttt tgtatcaaaa cgtttttcgg tagaagcacc aagaaacaaa
      721 ggcaaactgc cccaatcttt catgcgtttt tgcaatattt gataaccgat atcgtaacca
      781 attaaagctt cgtcaaatga aattggaatg gctgtttctg gagcaatcac caactttgta
      841 ttggtagtaa ttgttttatc cgccaaatcg gcaatacgat gcaattgttg gtcgggagta
      901 atggttgtaa atttgtaata tggatcaata ttaggttgaa caactacaat ttctgctgct
      961 gtcccctcat ccgaatgcaa agaatacatc cacaaagaag ttgaaacagg taataataaa
     1021 atgactactg gcaaggcaat tttctttggt tgtaaagttg ttttagtttc actaaagaaa
     1081 tacgttgaaa gtgctttaaa caacaaataa ttgacaagca acacccacaa acttccaccg
     1141 agcacacctg taaattcata ccattgcacc caagatggtg taattgaaaa cacattaccc
     1201 aaggttagcc aaggccacga aagctcccaa ttaaagtgta agtattcaaa tcccaaccaa
     1261 acactaacaa gggttgccaa tccatagttt gcaccaacgt gttttttaat aaaatgataa
     1321 atttgaaagg caattgccat taaaagtgaa ttcaacacaa aagccataat tgcaccattc
     1381 aaatcggcat tccaaatcca ccatgttgtt ccaatgttgt aaatgaaaaa cgaaatgtag
     1441 gcgtgaataa atactttaga cgatttatag cgttgctttg caatttgatg ttcaaccaac
     1501 aacagtggaa ttaaagcaat aaaaatcaat ggggttaaac ttcctgtata gggaaatgat
     1561 aacgttaaaa gtactcccga taatacgctc aataaatagc gccagcttct tgtcattctc
     1621 ataggagcaa atttaattgg aaaaaataaa taagcattga aaagaggaat taattatgcg
     1681 ttggattcaa tggaaaatcg gataatactt tgtatttaga gccttgcaat ttcataatat
     1741 ccttccacaa attatcgttg tttgtatcca taatgtgttg aatagtatga ttatccacta
     1801 caatccaggc atgttgttcc aattcttctt ctaattgacc accactccat ccagaatatc
     1861 cagcaaaaaa acgaactttc gatgcattgt ctggttgcgt gcgaatggca ttaataattt
     1921 ctaaaaaatc accaccaaaa taaagattgt ccattacttt ttcacttcct tcaatgtttt
     1981 ccaactgatg aatgtagaac aaactagaac tatcaactgg accgccaaca ctaacacgac
     2041 cttcatagcc atctaattcc tgacttattt cgcttggtgt taattctaaa taattgttta
     2101 aaacaaatcc aaaagttcca tctgcattgt aatcacaaat aagtacaacc gaacgtgtaa
     2161 aaaaagcgcc catctcaaac ggatcggata agagtaatgc tcctttttta ggacgtaaat
     2221 tgtttttaaa tgaaaaatcc atattcaaca tgatttagtc aaatatagtt tattttaatt
     2281 agaaaaatct ttaattgaac tgaaaatagt taacaaactg tcaacaagca aaaaacatac
     2341 gaaaaacgag agctttctgg tacgccaaga agtatttttt gtcctttttt caattgacca
     2401 ccgttaaaaa gttcgtccaa cattaaataa atagacgctg cccctacatt tccttttgtt
     2461 gccaaattgg taaaccattt ttctttcgga atttcgatgc ccaattcaac catttcgttg
     2521 tacatcttat cttcaaagaa aaaactcgac atgtgtggta ggaaataatc tatttcagaa
     2581 gacgaaattt gtttatcgtc cattaagcct tttaatttct taaaccctaa ttttacaatg
     2641 ttggcaccca acaatttcac atcttgttta atgctaaaca ccgattgctc aatgctttct
     2701 tgcgggtcaa aatctaaata actaattaat gaaccatcct cgcgtttttc tgcccccatg
     2761 tacatacacg gttcaatttc attg
//
LOCUS       DAOQVQ010000319         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_42952_length_2783_cov_3.158724, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000319 DAOQVQ010000000
VERSION     DAOQVQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42952_length_2783_cov_3.158724"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKN22_08970"
     CDS             complement(<1..789)
                     /locus_tag="PKN22_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNS42877.1"
                     /translation="MSREELLKKIDEKYAAMGQNADVHLSGLLYAQPITYWDFAQVDA
                     LLSLQTKRTTEPDEMVFIMYHQINELLFKMILWEIQQVGYCENISPKKFAMHLGRISR
                     YFDMLSNSFDIMKLGMEVEQFMKFRDTLTPASGFQSAQYRKVEIASTDLLNLIDFRDR
                     ATIDRNTPYEHAFDHMYWRAAGYNRQTGEVSTLLTLFEGKYKKELVEFMKEYNTVNLL
                     AKYKSLPEEYRNDLELINAMRHYDHTVNISWVMHHYDAARHYIES"
     gene            1075..1698
                     /locus_tag="PKN22_08975"
     CDS             1075..1698
                     /locus_tag="PKN22_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS42878.1"
                     /translation="MEDLRQISEFKTSPELVENLIQHSIHKTYKAGSIIPNENSYIRS
                     IPIVIKGTLKVIRTEDDGREILLYYIKAGESCIMSFLGGLHNETSKVKAEVEEEAEIL
                     FLPMEKVSIFIQEHPEWLDYIFRLYHKRFEELLDIVNAVTFKKVDERLLNLIKKKAEL
                     TNSNTISITHEQLANELATARVVVSRLLKQLEESGLVKLGRNKITLV"
     gene            1755..2549
                     /locus_tag="PKN22_08980"
     CDS             1755..2549
                     /locus_tag="PKN22_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS42879.1"
                     /translation="MLILGYFISIIIGVSLGLIGSGGSILTVPVLVYLFNVDVVSATA
                     YSLFIVGITSLVGSVSYFKKGLVNRKTALIFGIPSIISVFLTRAYILPAIPPVPLQSI
                     GINIQKDLFLLALFSILMVVSSFSMIRKTKSDTAITHEVQQFNYPLILIEGVVVGILT
                     GLVGAGGGFLIIPALILLSKLPMKEAVGTSLAIIAAKSLIGFMGENELSAIDWTLLLS
                     VTLFAIVGIFIGIALSKKIDGARLKPILGWFILVMGIYILIKELFL"
     gene            2628..>2783
                     /locus_tag="PKN22_08985"
     CDS             2628..>2783
                     /locus_tag="PKN22_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS42880.1"
                     /translation="MVVEQIYTGCLAQGAYYITSNGEAAIIDPLRETQPYLERLKKDN
                     VKLKYIFE"
BASE COUNT          923 a          445 c          471 g          944 t
ORIGIN      
        1 actttcaatg taatgacgag ccgcatcata atggtgcatt acccaagaaa tatttacagt
       61 atggtcgtaa tgacgcatgg cattgatgag ttccaaatca ttgcggtact cttctggaag
      121 tgatttgtat tttgctaata aattaacggt gttgtattct ttcataaact ccaccaattc
      181 ttttttatac tttccttcaa acaaggttaa caatgtagaa acctcaccag tttgacgatt
      241 gtatcctgca gcgcgccaat acatgtggtc aaaagcatgt tcataaggcg tatttctgtc
      301 aatggttgct ctatctctaa aatcaattaa gttcagcaaa tctgtagatg caatttcaac
      361 ttttcggtat tgtgcacttt gaaaaccact tgcaggagtc aacgtatctc taaatttcat
      421 aaattgctcc acttccatcc ctaatttcat gatatcgaag gagttggaca acatatcaaa
      481 gtagcgacta attcttccca agtgcattgc aaatttctta ggtgaaatat tttcacaata
      541 gccaacttgt tgaatttccc aaagtatcat tttaaataat aactcattga tttggtgata
      601 cataataaac accatttcat cgggttctgt tgtgcgtttg gtttgcaaag acaacaaagc
      661 atctacttgg gcaaaatccc aataagttat aggttgcgca tacaacaagc ccgataaatg
      721 cacatcggca ttttgaccca ttgcagcata tttttcatct atttttttaa gtaattcctc
      781 tctactcatc atagcgtttt cattttaaat ttagttttat caaatgtaat tgattcttgc
      841 tttgtttagt tttaaaaaat taacacacaa cgtaaaaata cgtattattt tcaacacaaa
      901 acacctatcc aattgattta atgcaactta aattacaatg cctatggtta aatttgttaa
      961 ataaatttca taaaacttac actttgttat atttcacact ttcaatcgta ttaaaaacca
     1021 agaattaaca gctttattgg atgattttag aaacaatttg ataattttac ccaaatggaa
     1081 gatttacgtc aaatttcgga gtttaaaact tcacctgaat tggttgagaa tttaattcaa
     1141 cacagtattc ataaaacata caaagcaggg agtattattc ctaatgagaa ttcttacatt
     1201 cgttccatac ccattgttat caaaggaact ttaaaagtta ttagaacaga agatgacgga
     1261 agagaaatac tactttatta cattaaagcg ggagaaagtt gtattatgtc ttttttaggt
     1321 ggacttcata atgaaactag taaagtaaaa gccgaagttg aggaagaagc tgaaattttg
     1381 tttttaccaa tggaaaaagt ttcaatattc atccaagaac atcctgagtg gttggattat
     1441 atttttaggt tataccacaa acgttttgaa gagttgttgg acatagtaaa cgccgttacc
     1501 tttaaaaaag ttgacgaaag gctattgaat ttaatcaaaa agaaagctga attaaccaac
     1561 agcaatacca tttcgataac gcatgaacaa ttagccaatg aacttgcaac tgccagagtg
     1621 gtggtgtcac gtttattaaa acaattagaa gaaagtggat tagtcaaatt aggaagaaat
     1681 aaaattacac tagtgtgact taagtaacag aaaacgtttg tgaagttgtg caattttgct
     1741 gaaaatttca taaaatgttg attcttggct attttatatc gataattatt ggagtatctt
     1801 taggtttgat tggtagcggt ggcagcattc tcactgttcc agtattggta tatcttttca
     1861 acgttgatgt ggtatcggca acagcttatt ctcttttcat tgttggtatt accagcttgg
     1921 ttggctctgt ttcttacttt aaaaaagggc ttgtcaatag aaaaacagca ctaatatttg
     1981 ggattccttc aataatttct gttttcttga caagggctta tattttgcct gccatccctc
     2041 ctgttccact acaaagtata ggcatcaaca ttcaaaaaga cctgttttta ttagcgttat
     2101 tctctatatt aatggttgtt tcttcattta gcatgatacg caaaacaaaa agtgatacgg
     2161 caataaccca tgaagttcaa caattcaatt atccacttat actgattgaa ggtgtagtgg
     2221 ttggaatcct aacgggatta gttggtgcag gaggtggttt tttaattatt ccagcattaa
     2281 ttcttctttc aaaactacca atgaaagaag cagtaggtac atctttagca atcattgccg
     2341 caaaatcttt gattggtttt atgggtgaaa acgaattaag tgcgattgat tggactttgc
     2401 tactaagcgt aactttattt gcaatagtcg gaatatttat aggaattgcg ttatctaaga
     2461 aaattgatgg agcgcgtttg aagccaattt taggatggtt tattcttgta atgggcattt
     2521 acattttaat aaaagaactt tttttataaa aaaattagtt gtgtaacata agtaactgta
     2581 caggatatta ttttgtaaca actttgcact aataaaatta taaaatgatg gtagtagaac
     2641 aaatttacac aggatgtttg gcacaaggtg cctattacat cacatcgaat ggtgaagcag
     2701 caattattga cccattgcgt gagactcagc catatttaga gcgattgaaa aaagacaacg
     2761 taaaacttaa atacattttt gaa
//
LOCUS       DAOQVQ010000320         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_43111_length_2776_cov_3.142962, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000320 DAOQVQ010000000
VERSION     DAOQVQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43111_length_2776_cov_3.142962"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKN22_08990"
     CDS             complement(<1..224)
                     /locus_tag="PKN22_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42881.1"
                     /translation="MKLAKLTLVGLALMAVNVVSAQAEVKKETSTELEKKPATEQKQT
                     LEVKRVGNTPVAKSQIQRTTVQRKPEAEVN"
     gene            451..1779
                     /locus_tag="PKN22_08995"
     CDS             451..1779
                     /locus_tag="PKN22_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNS42882.1"
                     /translation="MTNLFEEFSKPSYNDWVNKLKQELKGQSEELIQRKDEVEELYFN
                     SYQHQDFGNTQAAIAFSNAAIRGTYAPNNNWENMATIVVSDEKEANQQALAFLNMGVS
                     ALRFVVEHSTNWQVLTNGIETQYIETTFVVKNCATYFSALEYFNTNNTTLFFEMEPKS
                     LSTEDWEKLALSLKSQPRFAIIANGYAVQQCGATTWQEVGFMLATAHEALVQLMENGL
                     TCDQAAACIHFNVGAGAKYFYEIGKIRALRVLWAKIVEAYRPQNGATLNARITGITGF
                     SNKSLKDPYTNLLRQTTEAMSLLFAGVHAICVQPYDANSTNKATKLSTRMAINIPLLL
                     KEESYVDKVVDPLGGSYAVEYLTNEIAEKAWKTFQQIEELGGISSQNAIAFISNAVEQ
                     KATLRIEQLASKKDSLIGITIFPNPTEEENNWLSFDNYLGMKQLIFEQSI"
     gene            1786..>2776
                     /locus_tag="PKN22_09000"
     CDS             1786..>2776
                     /locus_tag="PKN22_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNS42883.1"
                     /translation="MRKNFTNIDWKSSSVDANNQPAEVNFTTAEKIDIKNYYDKKDIE
                     SIEHIGFVSGIAPNLRGPYGSMFAVRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLS
                     VAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVLDMKVLFDQIPLDEMSVSMTMNGAVI
                     PIMAFYIVAAEEQGVKQEQLSGTIQNDILKEFMVRNTYIYPPEPSMKIIADIFEYTSK
                     YMPKFNSISISGYHMQEAGATAAIELAYTLADGLEYLRTGVKAGINIDDFAPRLSFFW
                     AIGMNHNMEIAKMRAARMIWAKLVKQFNPKSDKSLALRTHCQTSGWSLTEQDPF"
BASE COUNT          908 a          514 c          519 g          835 t
ORIGIN      
        1 ttattcactt ctgcttcagg ttttctttga acagttgtac gctgaatttg tgattttgca
       61 accggtgtat ttccaacacg cttcacttct aaagtttgtt tttgttctgt tgccggtttt
      121 ttctcaagtt ctgttgaagt ttctttttta acttctgctt gagcgctaac tacattaacc
      181 gccattaatg ctaaacctac taaagtcaat tttgctaatt tcataatcaa aaattttaat
      241 cttttatttt tttgacaaac acaatgccaa aaattgtagt tttctaaatt ctaaccaaaa
      301 atataaataa atcttaacag aatcaatata tctgagcttt gtcacattat tttatttatt
      361 tttgacagaa attaacacct attgttgatt caagatgcct tgccgttact tttttgcaac
      421 tattcaacgt attaatttaa aaatgtgagg atgactaatt tgtttgaaga gttttcaaaa
      481 ccaagttaca acgactgggt aaacaaatta aaacaagaat taaaagggca atctgaagag
      541 ttaattcaga gaaaagatga agtagaagaa ctttatttca attcttacca acaccaagat
      601 tttggcaaca cacaagcggc tatagctttt tccaatgcag caatcagagg aacatatgca
      661 cccaacaaca attgggaaaa catggcaact attgttgtaa gcgatgaaaa agaagccaac
      721 caacaagctt tggcgttttt aaacatgggt gtttctgcct tgcgttttgt agtggaacat
      781 tctaccaatt ggcaagtgtt aaccaacggt attgaaacgc agtacatcga aactactttt
      841 gtagtaaaaa attgcgccac atatttttct gctcttgaat atttcaacac aaacaatact
      901 accctattct ttgaaatgga accaaaatca ctttctacag aagattggga aaaattagca
      961 ctatcgttga aatcacaacc tcgctttgca attattgcaa atggttatgc ggtgcaacaa
     1021 tgcggtgcaa ctacctggca agaagtaggt tttatgcttg caacggctca cgaagctttg
     1081 gtgcaattaa tggaaaacgg tttaacttgc gaccaagcag cagcatgtat tcattttaat
     1141 gttggtgctg gtgcaaaata tttttacgaa attggaaaaa ttagagcctt gcgcgtattg
     1201 tgggcaaaaa ttgtagaagc ctatcgaccg caaaatggag caactttaaa cgctcgaatc
     1261 acaggaatca caggatttag caacaaatcg ttaaaagatc cttacaccaa tttattgcga
     1321 caaacaacag aggcaatgag tttgttattt gcaggtgttc atgccatttg tgtgcaaccc
     1381 tacgatgcca attcaaccaa taaagcgact aaattatcaa cacgtatggc catcaacata
     1441 cctttgttgc taaaagaaga aagttatgtg gataaagtgg ttgaccctct gggcggtagt
     1501 tatgctgttg aatatctgac caatgaaatt gcagaaaaag cttggaaaac cttccaacaa
     1561 atagaagaat tgggcggaat ttcttcacaa aatgcaattg catttatttc caatgccgtt
     1621 gaacaaaaag caactttacg cattgagcaa ttggcaagca aaaaagacag tttgattgga
     1681 attacaattt tccccaatcc aaccgaagaa gaaaataact ggttgtcttt tgataattat
     1741 ttaggaatga aacaattgat ttttgaacag tccatataat aaagcatgag aaagaatttt
     1801 acaaatatcg actggaaatc gagctctgtt gatgccaaca atcagcctgc tgaagtaaat
     1861 ttcacaacag cagaaaaaat tgacatcaaa aattattacg ataaaaaaga catagaatct
     1921 atagaacaca ttggatttgt ttctggaatt gctcccaatt taagaggtcc gtatggttct
     1981 atgtttgccg tgcgtccgtg gaccattcgt cagtatgctg gtttttctac tgccgaagaa
     2041 tccaatgcat tttaccgcag aaatttggcg gctggtcaaa aaggactttc agttgcattt
     2101 gacttagcaa cgcaccgtgg atatgactcc gaccaccctc gtgttgttgg agatgttgga
     2161 aaagcaggtg ttgccattga ttcggtgttg gacatgaaag tattgttcga ccaaattcct
     2221 cttgatgaaa tgtcggtttc aatgaccatg aacggtgctg taattcctat catggcattt
     2281 tacattgttg cggcagaaga acaaggggtg aaacaagaac aattatcggg aaccatccaa
     2341 aacgatattc ttaaagagtt catggtgcga aacacttaca tttacccgcc agaaccttcc
     2401 atgaaaatta ttgcagatat ttttgaatac acttcgaaat acatgcctaa attcaattcc
     2461 atttctatct caggatacca catgcaagaa gcgggagcaa ctgctgccat tgagttggcc
     2521 tataccctag ccgatggttt ggaatatttg cgcacaggtg ttaaagcagg catcaacatt
     2581 gatgattttg ctccacgttt gagttttttc tgggcaattg gaatgaacca caacatggaa
     2641 attgcaaaaa tgcgcgcagc acgtatgatt tgggcaaaat tggtaaaaca attcaaccct
     2701 aaaagtgata aatcattagc gttgcgcacc cactgtcaaa cttctggttg gagtttaacc
     2761 gagcaagatc cgttta
//
LOCUS       DAOQVQ010000321         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_43387_length_2763_cov_3.851182, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000321 DAOQVQ010000000
VERSION     DAOQVQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43387_length_2763_cov_3.851182"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1403
                     /gene="mnmE"
                     /locus_tag="PKN22_09005"
     CDS             42..1403
                     /gene="mnmE"
                     /locus_tag="PKN22_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNS42884.1"
                     /translation="MTALQNDVICALATPTGIGAIGVIRISGKDCLQLISPIFSKNLA
                     DKQSHSLHFGTISTKNGEIIDEVLISVFKDGKSFTGEESAEIACHGSNYILQRVLGLL
                     LEQGCRMAEPGEFTMRAFLNGKMDLSQAEAVADLIASESKKAHEIALNQLRGNFSNEL
                     KDLREQLIHFASLVELELDFSEEDVEFADRTELLNLIIEVLNVITRLEQSFELGNAIK
                     NGVPVAIVGAPNTGKSTLLNQLLQEDRAIVSNIAGTTRDVIEETLNIDGILFRLIDTA
                     GIREHAEEIEALGIERSKQKIEQAKLVLCLADLSVENSLQEVRLWKKELQEKHPSKRI
                     VLIANKADVLSVELEENEISISAKQGQNMEVLKAWMVEQIVQGTDLQNEVVISNVRHR
                     DALTRTREALQQAKIGLETNITGDFVAMDIRQAMYHLGTITGNISEDDLLANIFSKFC
                     IGK"
     gene            complement(1416..2297)
                     /gene="lipA"
                     /locus_tag="PKN22_09010"
     CDS             complement(1416..2297)
                     /gene="lipA"
                     /locus_tag="PKN22_09010"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNS42885.1"
                     /translation="MDSTKEIDGSKVRIKKPNWLRVKLPTGESYREVRSIVSEHKLHT
                     ICESGSCPNMGECWGEGTATFMILGNICTRSCGFCAVQTGKPDAVDEFEPGRVAHSVK
                     LMNLKHAVITSVDRDDLKDGGSLIWAQTVRAIRAQSPGTTLETLIPDFAGKWENLQNV
                     IDVAPEVVSHNLETVRRLTKAVRIQAKYDRSLEVLFRLKKGGMRTKSGIMLGLGESHE
                     EIIETLDDLASVNVDIVTLGQYLQPTPKHLPVAEFVTPERFAEYRDYALKLGFKYVES
                     GPLVRSSYHAEKHVADL"
BASE COUNT          833 a          543 c          565 g          822 t
ORIGIN      
        1 tgttgaaaaa gaaatagaaa agattaaagt aactttgttg aatgacagcg cttcaaaatg
       61 atgtaatttg tgccttggca actccaacag gaattggagc aattggcgtt attcgtattt
      121 caggaaaaga ttgtttgcag cttatttcgc ccatttttag taaaaattta gccgataaac
      181 agtcacattc tttgcatttt ggtaccattt ccacaaaaaa tggtgaaatc atcgatgaag
      241 tattgatttc tgtttttaaa gatggaaaaa gttttacggg cgaggaaagt gcagaaattg
      301 cttgtcacgg ttccaactac attttgcaac gtgtattagg tttgttgttg gagcaaggtt
      361 gccgaatggc tgagccgggc gagtttacca tgcgtgcatt tttgaatggt aaaatggacc
      421 tctcacaggc agaagctgtt gccgatttga ttgcttccga atctaaaaaa gcccatgaaa
      481 ttgccttgaa ccaattgcgt ggtaatttct ccaacgaact caaagatttg cgcgaacaac
      541 tcattcattt tgctagtttg gtggaattgg agcttgattt ttcggaagaa gatgtggaat
      601 ttgccgacag aacagaactg ttgaacctca taatagaagt gttgaatgtc attacccgcc
      661 ttgagcaatc gtttgaattg ggaaatgcca tcaaaaatgg agttccggtt gccatagttg
      721 gagcacctaa cactggtaaa tccactttat tgaatcaatt gttgcaagaa gaccgtgcca
      781 ttgtgagcaa cattgctggt accacgcgcg acgtaattga agaaacattg aacattgatg
      841 gaattttatt ccgcttgatt gatacagcag gtattcgtga gcatgccgaa gaaattgaag
      901 cattgggcat tgagcgttct aaacaaaaaa ttgaacaagc caaattggtg ctttgtttag
      961 ccgatttaag tgttgagaac agtttgcaag aagtgcgctt gtggaaaaaa gaattgcaag
     1021 aaaaacaccc ttccaaacgc attgtgttga ttgccaataa agccgatgtg ctttcggtag
     1081 aattagaaga aaatgaaatc agtattagtg ccaagcaggg acaaaacatg gaagtgctaa
     1141 aagcgtggat ggtggaacaa attgtgcaag gtactgattt acaaaacgaa gtagtaatta
     1201 gcaatgttcg tcaccgcgac gccctcacac gcacacgcga ggcattgcaa caagcaaaaa
     1261 taggattgga aaccaacatt actggcgatt ttgtggcgat ggacatccgc caagcgatgt
     1321 atcatttagg taccatcaca gggaatatct cagaggacga cctattggcc aatattttta
     1381 gtaaattttg cattggcaaa taaatctatt cgcaactaca aatcggctac gtgtttttct
     1441 gcgtggtaag aagaacgaac aagtggtccg ctttcaacgt atttaaagcc taattttagg
     1501 gcataatcgc ggtattctgc aaaacgttct ggagttacga actcagccac tggcaagtgc
     1561 ttaggcgttg gttgcaagta ctgtcccaag gttacaatat ccacattaac agacgctaaa
     1621 tcgtcgagtg tttcaataat ttcctcatgt gattcgccca atcccaacat aattcccgat
     1681 ttggtgcgca ttccaccttt ttttaagcgg aacaacactt ccaaactacg atcgtatttt
     1741 gcttgaatac gcactgcttt ggtcaaacgt cttacggtct ccaaattatg cgaaaccact
     1801 tctggtgcaa catcaataac attttgtaaa ttttcccatt ttcctgcaaa atccggaata
     1861 agggtttcta aggttgtgcc tggtgattgt gctctaatag cacgcacagt ttgcgcccaa
     1921 ataagcgagc ctccgtcttt taaatcatca cggtcaaccg aagtgataac ggcatgtttt
     1981 aaattcatca acttcacaga atgtgccaca cgacccggtt caaattcatc cactgcatct
     2041 ggttttcctg tttgcacagc acaaaacccg caggagcgcg tacaaatatt tcccaaaatc
     2101 ataaaagtag ccgttccttc tccccagcac tcacccatat tggggcaact accactttcg
     2161 caaatggtat gcagtttatg ttcagacaca attgatcgaa cctcacggta gctctcacct
     2221 gttggcaatt tcacgcgcaa ccaatttggc tttttgatgc gcactttcga cccgtcgatt
     2281 tcttttgtac tgtccatttc tgcttgagtt gataattcag gtgcaaattt actaaaatta
     2341 acataaatgg aagtgaaatc acctatcgtt tacaaaatag caactatttt tttgcaattt
     2401 attttaaaaa caaactgcat tttttgacct atatcaaata attcaatgcg ttgttttttg
     2461 tccactctta atcattatat ttgaatacaa aacaatcatc aattttctac tgttatacta
     2521 cttggatagc tactgcatat acatgcaatt gagagctatt ctattttaat gtgtatttac
     2581 aaataattta gaaaatgaaa actatatact attcaatttc ccttatttta agtgtgtttt
     2641 tgtcgtttgt gggttggggg cagcttttaa actcaacaac agcaggtgca ggtacatgga
     2701 cagccacaac aactgagtgt gttaccatag aactatgggg cggcggcgga ggtggcggtt
     2761 aca
//
LOCUS       DAOQVQ010000322         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_43451_length_2760_cov_3.736414, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000322 DAOQVQ010000000
VERSION     DAOQVQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43451_length_2760_cov_3.736414"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKN22_09015"
     CDS             complement(<1..252)
                     /locus_tag="PKN22_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42886.1"
                     /translation="MKYTLIALAAFFVHFSGFSQRDDHSKYFVKFSPTQLIVGEMNFA
                     FEHRVAPQSSLEVSFGPTVSEIGINKIYFDNVLNNNNATR"
     gene            complement(304..1296)
                     /locus_tag="PKN22_09020"
     CDS             complement(304..1296)
                     /locus_tag="PKN22_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS42887.1"
                     /translation="MKSRLLVGLFSLVGAYTFSQTNARFSQFNVAKGLMNPAAIGVEA
                     KISAELIYRTQWTGQKGAPQSVGFMGSYEINNSHAVGLSVLNDQVGIAKSTGINLGYA
                     YRIYINDEQNFAFGASLGVQNVNNDYSRLFLINQNDPAFLQSYNQWKFNAGFGMFYNG
                     PKMYIGYSIPQLFNNIHSGPNNGFRINRWHHYLAAGFYISNHDASYIFNPCVQVKFVP
                     NAPIQGDLLLRNIINGKFAIAVGYRTENAIQAGFDVMISNNARIGYNFNYNFGRYSRF
                     MATSHEVYIGMGLPFYYETNRFAKRQYTNNKQGFSSDYKQRSRKLNKRNKFKPF"
     gene            complement(1299..2528)
                     /locus_tag="PKN22_09025"
     CDS             complement(1299..2528)
                     /locus_tag="PKN22_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS42888.1"
                     /translation="MLRRLLVARIGLIGFFTLLVQFSFACAVDVTIVQGNAITLCPNS
                     TNAITAAAGYVSYTWSGPATGSGSAATATGNGWVVVTATDGVGCVSKDSILVTLYPDP
                     AASLSSSEGLNVCPTVGTTTLSLNQAYNSYLWNDGSTGATLSVTQSGLYSVEVTDANG
                     CKDTANITIDFIDFSLTAVGGNNVCAGSFVMLQASGGDVYAWSTNEFSSSIVVAPTQS
                     STFSVSIYKGACHTTLSTTVDVIALPPHSMPDTMLVFPGQVPYVFGPADFDTYTWAPA
                     DLVTDTSGVRTGYIGTGDGVVTLVATSNSLGCSMGHDIYFKMIDLTIPEGFSPNGDGI
                     NDKFEIPEIIRFEAKLKVWNRWGDVVFESDRYLSDWDGTCQSSFCMGKDVLPEGTYFY
                     KLTIEKYEFSGYLTLKL"
BASE COUNT          912 a          571 c          473 g          804 t
ORIGIN      
        1 ccgtgttgca ttgttgttgt ttagaacatt atcaaagtaa attttattaa ttcctatttc
       61 agaaaccgtt ggtccaaaag aaacttccaa actcgattga ggtgcaacac ggtgttcaaa
      121 tgcaaaattc atttcaccaa caattaattg tgtaggcgaa aattttacaa aatactttga
      181 atgatcatcg cgttgtgaaa aaccactaaa atgaacaaaa aaagcagcaa gggcaattaa
      241 agtatatttc ataattctac tattagttta taggttaaac gaatcaaacg ccaatctatt
      301 gttttagaaa ggtttaaatt tattgcgttt atttaacttt cttgaacgct gtttataatc
      361 gcttgaaaaa ccttgtttgt tgttggtata ttgacgctta gcaaaacggt tggtttcata
      421 ataaaacggc aatcccattc cgatatacac ttcgtgcgat gtagccataa agcgtgaata
      481 gcgaccaaaa ttatagttga agttataacc aatgcgtgca ttattagaaa tcatcacatc
      541 aaaacctgct tgaatggcat tttctgtgcg ataaccaact gcaatagcaa attttccatt
      601 aataatgttg cgcaacaata aatcaccttg aattggagcg ttgggaacaa atttcacttg
      661 cacacatgga ttgaaaatat acgatgcatc gtggttggat atgtaaaaac ctgctgctaa
      721 ataatggtgc caacggttga ttctaaaccc attgttaggt cccgaatgaa tgttattaaa
      781 caattgcgga atggaatacc cgatgtacat ttttggtccg ttgtaaaaca ttccaaatcc
      841 tgcgttgaac ttccattgat tgtaactttg caagaaagcc gggtcatttt ggttaatcaa
      901 aaataaacgc gaataatcgt tgtttacatt ttgtacgcct aaacttgctc cgaatgcaaa
      961 attttgttca tcgttgatat aaatacggta ggcatatcct aaatttattc ctgttgattt
     1021 ggcaataccc acttgatcgt ttaaaacaga aagcccaaca gcgtgtgagt tattgatttc
     1081 atacgacccc ataaagccaa cggattgcgg cgctcctttt tgaccagtcc actgcgttct
     1141 ataaattaac tcggcagata tttttgcctc cactcctatt gcggcaggat tcatcaatcc
     1201 tttggcaaca ttgaattgcg aaaatcgagc gttggtttgc gaaaatgtgt aagccccaac
     1261 caaactgaat aatcctacta gtaatcttga tttcattgtt aaagttttaa tgttaggtag
     1321 cctgaaaatt catatttttc gatggtcaac ttatagaaat aagtgccttc tggcaacaca
     1381 tctttcccca tgcaaaaact agattgacac gttccatccc aatcacttag gtaacgatcg
     1441 gattcaaaaa ccacatcgcc ccaacggttc caaactttca atttggcttc aaaacgaatg
     1501 atttctggaa tttcaaattt atcgttgata ccatcaccgt taggagaaaa cccttcggga
     1561 atggtaaggt caatcatctt aaaataaata tcgtgcccca ttgaacaacc caatgaattg
     1621 cttgttgcaa ctaaagtaac cactccatct ccagtgccaa tataacccgt tctaacacca
     1681 cttgtatctg taaccaaatc agcaggtgcc caagtgtaag tatcaaaatc ggcaggacca
     1741 aaaacataag gaacttgtcc tggaaaaacc aacattgtat ctggcattga atgaggtggt
     1801 aatgcaatta catccaccgt agttgaaaga gttgtgtgac aagcgccttt gtaaatgcta
     1861 acagaaaatg tggatgattg agttggagct acaactatgg aagacgaaaa ttcattggtt
     1921 gaccatgcat aaacatctcc accagacgct tgaagcatca caaaagagcc tgcacaaaca
     1981 ttgtttccac ctacagcagt caatgaaaaa tcaataaaat cgatagtgat atttgctgta
     2041 tccttgcaac cattggcatc ggtaacctca acactataca aaccagattg cgttacagac
     2101 aaagttgctc ctgtacttcc atcgttccac aaatacgagt tatacgcttg atttaggctc
     2161 aaagtagttg tcccaactgt tggacaaaca ttcaatcctt cactggaact caaagatgct
     2221 gctggatctg gatacaaagt aaccaaaata gaatctttag aaacacaccc tactccgtcg
     2281 gttgcagtaa caactaccca accattacct gttgcagttg ctgcactacc acttccggtg
     2341 gctggtcccg accaagtgta ggaaacatat cctgccgcag ctgttatggc gttggtacta
     2401 ttgggacaca atgttattgc atttccttga acaatggtta catcaaccgc gcaagcaaaa
     2461 gaaaattgca ccaagagtgt aaaaaatcca atcagtccaa ttcttgctac taaaagtctt
     2521 ctcaacatat attaaagttc agtttattac aaaaataagc taataaattt gaatagaaac
     2581 caaactccca caaaaaagac ttaaaatcgg ctacttaata acaattaatt tggcagattt
     2641 ttctgtaatt ccatcgcgaa taaccaccat ataatgacca tttaccaatg agctaatgtc
     2701 aatttgcacc gtatgttcgc catccaattg tttgttggca aaaacttcgg caacaacagc
//
LOCUS       DAOQVQ010000323         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44046_length_2736_cov_3.416636, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000323 DAOQVQ010000000
VERSION     DAOQVQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44046_length_2736_cov_3.416636"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2736
                     /locus_tag="PKN22_09030"
     CDS             <1..>2736
                     /locus_tag="PKN22_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNS42889.1"
                     /translation="AHDLSEVVVTDNKDWQKHAIHLIGGSDLSQQASFFSQMESMRAT
                     LESDKFGGKVHTLKRQSDDPVPPTSIQQIMNRIAQGVSLMTYYGHYGINSGGFEINID
                     EVQNWNNTGKYPFMLVNSCYNGDIFQPGLNSSSEYFVNAHNLGAIGYLSSVSTGFHPM
                     VGIYSNQLYNQFCNFSYGKSISYNMKNAIQSIETPNNIYMEVTCTQMLLNGDPALKIN
                     YHNKPEIELTEENVFFKPDFIDLNTDSIEMNIVLKNLGQSIIDTFNIEIRRDFPGTTT
                     DSLYNIKRARLDYVDTIKFKFPLQPSISAGLNSFDIKVDIPSFIPEQYDEFNNNQIIK
                     TYFLRLDGILPVVPYEFAVVPNDTMTLKASTIDPIAPFNTYLFQIDTTDLFNSPFLKN
                     AVVTGTGGVKEVKPNQWDSPLQFTDSTVYFWRVAVDEPNPNWSESSFQYIKGKSGWGQ
                     DHFFQFKKNTFNNVLYDRNQRLRKWVPDSIQLTCDVFNDIRYENAFYLNGTLQDYSVC
                     SWTPPIHVAVIDPVTLESWGTNYNGANPTHDFGSDNCRTRVEKFFIFYQNNLTSLQNF
                     QNMVLNGVPDGHYLLIYTPIDTRYDWWNSIDSVNMYNTFATLGADSIYAGRANGPFAF
                     FTRKGYPGTVVENVIDPTNGNGAASGYTYVGITAYMPTSTEQGNEVSTLIGPAMKWGN
                     VYWKQDSIDPVNNADTTRLIIQPYQMDMTPGTPINMLFTSNDSLLNLENVVSSTQYPY
                     IRLQASYKDTTNFTPAQVDRWHVLYDLAPEAAIDGTNGYYLSHLNDSVYQGQTIEFAC
                     DIRNIFTVDMDSLLIKYWVEDKNHNKQFIQYPRQDSLRVGEMIRDTISFSTENMAGYN
                     SLWVEVNPYINGSLYVTDQPEQHHFNNILQIPFYVNADDVNPLLDVTFDGRHILNG"
BASE COUNT          888 a          504 c          503 g          841 t
ORIGIN      
        1 gcgcatgatc tcagcgaagt ggtggtcacc gataataaag attggcaaaa acatgcaatt
       61 catttaattg gtggtagtga tttatcacaa caagcatctt tcttttcaca aatggaaagt
      121 atgcgtgcaa cactggagtc ggataaattt ggtgggaaag tgcatacgtt gaagcgtcaa
      181 tccgatgatc ctgtacctcc aacaagcatt caacaaatca tgaaccgcat agcacagggt
      241 gtttcactta tgacgtatta tggacattat ggaataaaca gcggtggctt tgaaatcaac
      301 attgacgaag ttcagaattg gaacaatact ggaaaatatc cttttatgtt ggtcaattcg
      361 tgctacaatg gtgatatttt tcaacctgga ctgaattcct cttcggagta ttttgtaaat
      421 gcacataatt taggggcaat tggttacttg tcttctgtaa gcactggttt tcacccaatg
      481 gtgggtattt atagtaatca actttacaat caattttgta acttttctta tggaaaaagc
      541 attagttaca acatgaaaaa tgcaattcaa agcattgaaa cacccaacaa tatttatatg
      601 gaggtaacgt gtacgcaaat gcttttgaat ggcgaccctg cattaaaaat caattaccat
      661 aataaaccag aaattgaatt aactgaagaa aatgtttttt tcaagcctga tttcatagac
      721 ttgaataccg atagtattga aatgaacatc gtcttgaaaa atttagggca atccattatc
      781 gatacattta atatcgagat tcgaagagat tttccgggca caaccaccga ttctttatac
      841 aatatcaaac gcgcacgact tgattatgtt gatacaatta aattcaaatt cccacttcaa
      901 ccttccattt cagctggatt gaattcgttt gatattaagg tggatattcc gagttttatt
      961 cccgaacaat acgatgaatt taacaacaat caaatcatta aaacttattt cttgcgcttg
     1021 gatggtatct tgcctgtggt gccttatgaa tttgcagttg ttcccaacga tacaatgacg
     1081 ctcaaagcat caactattga cccaattgca ccgttcaata cctatctttt tcaaatagat
     1141 acaaccgatt tgttcaatag tccattttta aaaaatgctg ttgtaactgg aactggtggc
     1201 gtaaaagaag taaaacctaa tcaatgggat agtccgttac aatttacaga tagtactgtt
     1261 tatttttggc gtgtagctgt agatgaacct aatcccaatt ggtctgaaag ctctttccaa
     1321 tacattaagg gaaaatcggg ctggggacaa gatcatttct tccaattcaa gaaaaacaca
     1381 ttcaacaatg tattgtatga ccgtaatcaa cgtttgagaa aatgggtacc cgattctatt
     1441 caactgactt gtgatgtatt taacgacatt cgctatgaga atgcatttta tctcaatgga
     1501 actttacaag attattcggt ttgttcgtgg acaccgccaa ttcacgttgc tgtaatcgat
     1561 ccagtaacct tagaatcatg gggaaccaac tacaatggtg ctaacccaac acacgatttt
     1621 ggtagcgata actgtagaac cagagttgaa aaattcttta tattctacca aaataattta
     1681 accagtttac aaaactttca aaatatggta ctcaatggcg taccagatgg gcattaccta
     1741 ctaatttaca caccgataga tacacgatat gattggtgga acagtatcga ttcggttaat
     1801 atgtataaca cctttgctac cctaggtgct gatagcatct atgctgggcg tgccaatgga
     1861 ccgtttgcat tttttacacg aaaaggatat cctggaactg tagttgagaa tgttattgac
     1921 cctaccaatg gaaatggtgc tgcaagtggc tatacttatg ttggaattac agcatatatg
     1981 ccaacatcta ccgaacaagg aaatgaagtt tcaacactta ttggccctgc aatgaaatgg
     2041 ggcaatgtgt attggaagca agatagcata gaccctgtaa acaatgcaga tacaacacgt
     2101 ttaatcatcc aaccctatca aatggatatg acaccaggga ctccaatcaa tatgctattc
     2161 acctccaatg attcgctgtt gaatttggaa aatgtggtta gttcaacaca atatccatac
     2221 attcgtctac aagcttcgta taaagacact accaatttca ctcctgctca ggtagataga
     2281 tggcatgttc tttatgactt agctccagag gctgcaattg atggcacgaa tggctattat
     2341 ttaagtcatt tgaacgatag tgtttatcaa ggtcaaacca ttgaatttgc ttgcgatatt
     2401 cgtaatattt tcactgttga tatggattca ttactcatca aatactgggt tgaagataaa
     2461 aatcataaca aacagtttat tcagtatccg cgtcaagatt cattaagagt aggagaaatg
     2521 attagagata ccatttcgtt ttcaaccgaa aatatggctg gatacaattc actttgggta
     2581 gaggttaatc catatataaa cggaagtttg tatgtaaccg atcaaccaga acaacaccac
     2641 tttaataaca ttttgcaaat tcctttttat gtgaatgcgg atgatgttaa cccgttgtta
     2701 gacgttacat ttgatggtag acacattctc aatggc
//
LOCUS       DAOQVQ010000324         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44102_length_2734_cov_3.363942, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000324 DAOQVQ010000000
VERSION     DAOQVQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44102_length_2734_cov_3.363942"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKN22_09035"
     CDS             complement(<1..204)
                     /locus_tag="PKN22_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNS42890.1"
                     /translation="MTKLISSKILDFTLTPKVDKVGVEERVARIQTRSLKKESKMQAL
                     QLALNMIDLTTLEGKDTPNKVRQM"
     gene            complement(314..1183)
                     /locus_tag="PKN22_09040"
     CDS             complement(314..1183)
                     /locus_tag="PKN22_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42891.1"
                     /translation="MTILSLFISCTIALSFPFSHFDDNHQNGDINKKDEEGRKQGKWI
                     YFGKDRPNSGYPAEGKIEEGSYVNDRKEGTWIKYYNDGITPKLKGEYKNNRPQGGYIK
                     LSPKGVVIERGTFTMGKYMDSLVRYHSDGTLLYQGYYNNEGNEQGTIKYYYPNGQVEF
                     EYTSDNGTPKGKATRYYENGDVKELIYYEGGAVTKSEKKEMVNTEVVVKNPEASKEMA
                     PSIKTPNTKGAKFLPNGYNKVYNANQEIWQDGDFRNGRLWDGKVYEYDKDGILLKVRV
                     FKNGVYHSDGQLN"
     gene            complement(1333..2283)
                     /locus_tag="PKN22_09045"
     CDS             complement(1333..2283)
                     /locus_tag="PKN22_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS42892.1"
                     /translation="MAKTLVIGAGGQIGTELVVALRNKKGNNNVIAADVKPSPSEGIQ
                     DGPYVQMDVLDRAFVREYIINNEVKEVYLLAALLSATAEKNPDFAWRLNMEGLFTILD
                     LAKEGHVEKIFWPSSIAVFGPTTPQENTPQYTVMEPTTVYGISKQAGERWCEYYHQKF
                     GVDVRSIRYPGLISYASLPGGGTTDYAVDIFYQAKKYNKFTSFLSENTRLPMMFMEDA
                     IRGTLELMDAPAENLTVRSSYNFSGCAFTPAELTQEIQHHLPTFETTYQPDFRQAIAD
                     SWPGSIDDSVARKDWGWKEKYGVKQLVQTMLENVDISKID"
     gene            2417..>2734
                     /locus_tag="PKN22_09050"
     CDS             2417..>2734
                     /locus_tag="PKN22_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS42893.1"
                     /translation="MFKAYKKDFWLLAFGMFLFMTSFNLLIPELNTMMENLNAGDKKG
                     LVFVFFSIAAAMARPIAGKLSDTIGRKKVMYGGVVIGALICLASPPIGLFFIFLLFRF
                     PHGF"
BASE COUNT          766 a          515 c          433 g         1020 t
ORIGIN      
        1 catttgacgg actttattgg gcgtatcttt tccttccaat gttgttaagt cgatcatgtt
       61 caatgccaac tgcaacgctt gcattttact ttcttttttg agacttcggg tttggatacg
      121 agctacacgt tcttctaccc ctactttatc aaccttagga gttagtgtga aatccaaaat
      181 ttttgagcta attagcttag tcataaattc tattttaatg taaaaataag atttttgaaa
      241 cttttacaaa ataaactttc aaaaaaaagg ccatccacgt gggacagcct ttaatatatc
      301 tataaaaata gtattaattc aattgaccat cagagtggta aaccccattt ttgaacactc
      361 ttacttttag caaaataccg tctttatcgt attcataaac tttaccatcc cacaatctac
      421 catttctaaa atcaccatct tgccaaattt cttggtttgc attataaact ttattataac
      481 catttggtaa gaatttagcg ccttttgtat ttggtgtttt aattgatggc gccatttctt
      541 tggatgcttc tggattttta accacaactt ctgtgtttac catttctttc ttttcagatt
      601 ttgtaacagc accaccttcg taataaatca attcttttac atctccattt tcgtaataac
      661 gtgttgcttt tccttttggc gtcccgttat ccgaagtata ttcaaattct acttgtccat
      721 ttgggtaata atatttaatt gtaccttgtt cgtttccttc attgttgtaa tatccttggt
      781 aaagtagtgt accatcactg tggtaacgca ccaatgaatc catgtattta cccattgtga
      841 aagtcccacg ttcaatcacc actccttttg ggcttaattt gatatatcca ccttgcggtc
      901 tgttgttttt atactcaccc ttcaatttag gagttatacc atcgttgtag tactttatcc
      961 aagttccttc cttacggtca ttaacataac taccttcttc gatttttcct tctgctgggt
     1021 aacctgaatt aggacgatct tttccaaagt aaatccattt accttgtttt ctaccttctt
     1081 catctttttt attaatatct ccattttgat ggttatcgtc aaaatgggaa aatgggaaac
     1141 tcaatgcgat agtgcaagag ataaagagtg atagtattgt cataagtaat ttttcgagtt
     1201 tcataataac caaatttata tagtaattat aaaatgagct gttttctgta tgcaagttaa
     1261 ggtatttttt tggaacagca atcatttaat ttgtgttttt agataaatac tttgtttttt
     1321 tcgatgaacg aattaatcaa tttttgaaat atctacattt tctaacatcg tttgaaccaa
     1381 ctgtttaaca ccatattttt ctttccaacc ccaatctttt cgggcaacac tgtcatcgat
     1441 gctaccaggc caagagtcgg caattgcttg tctaaaatct ggttgataag ttgtttcgaa
     1501 agttggtaaa tggtgttgga tttcttgtgt caattctgct ggtgtaaatg cacaacctga
     1561 aaaattatag gatgaacgca ctgtaagatt ttctgcgggt gcatccatta attcaagtgt
     1621 tccacgaata gcatcttcca taaacatcat cggtaaacgc gtgttttcgg ataagaaact
     1681 ggtgaatttg ttgtattttt ttgcttggta gaaaatatca actgcataat cggttgtccc
     1741 tcctcctggc aatgaagcat aagatattaa tcctggataa cggatactgc gtacatcaac
     1801 accaaatttt tgatggtaat attcacacca acgctcacct gcttgtttgg aaataccata
     1861 aaccgttgta ggttccatca ccgtatattg tggcgtattt tcttgaggag ttgttgggcc
     1921 aaaaactgca attgaactag gccagaatat tttttctaca tgaccttctt ttgctaaatc
     1981 taaaatggta aacaatcctt ccatgtttaa gcgccaagca aaatctggat ttttttcagc
     2041 agttgccgat aacaatgctg ccaataaata cacttctttt acctcgttgt tgatgatgta
     2101 ttctctaaca aatgctctat ctagcacgtc catttgaaca taaggaccat cttgaatccc
     2161 ttctgaaggt gaaggtttca catcggcagc aataacatta ttgttacctt ttttgtttct
     2221 taatgcaaca acaagctcag ttcctatctg acctcctgcc cctattacca atgttttagc
     2281 catattagtg tgtattgatg ttaaaaatac agcgcaaaaa tacaattttc aaagcgttta
     2341 agactatttt atcgtgttaa attttatgct tttttaaaac tttaaatcaa aaactaacat
     2401 atacttttgc atttcaatgt ttaaagcgta taaaaaagat ttttggcttc ttgccttcgg
     2461 aatgtttttg ttcatgacga gtttcaacct actcattcca gaactcaata ccatgatgga
     2521 aaatttgaat gcaggtgata aaaaaggatt ggtttttgtc tttttttcaa ttgcagcagc
     2581 tatggcacgg ccaatagcag ggaagctttc agataccatt ggtcgaaaaa aagtaatgta
     2641 tggtggagtt gttattggtg cgttaatttg tttagcctcc ccccccattg ggcttttttt
     2701 tatttttttg ttgtttcgtt ttccacatgg tttt
//
LOCUS       DAOQVQ010000325         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44208_length_2730_cov_4.361121, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000325 DAOQVQ010000000
VERSION     DAOQVQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44208_length_2730_cov_4.361121"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKN22_09055"
     CDS             complement(<1..381)
                     /locus_tag="PKN22_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HNS42894.1"
                     /translation="MARYVGLQSQINRNNQKSILLLAAFPAMILVGVYLVLFFLTQED
                     TTSTNELFLAYLPYVLGVVGIWFVIAYFAHTSLIQHATHSKPLERSENLRVYNLTENL
                     CMSVGMKMPKLFIIETNALNAFASG"
     gene            complement(391..951)
                     /locus_tag="PKN22_09060"
     CDS             complement(391..951)
                     /locus_tag="PKN22_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNS42895.1"
                     /translation="MTGLIVIGIIVVLVIWAIALNNKLVALKNNRENAFADIDVQLKQ
                     RHDLIPQLIGAVKGYMEHESSVLTKVTEMRAKAMNATDINSKIAAEQELDSAMKAFNV
                     QVESYPDLKASANFLQLQGEVSDIENKLAAVRRYFNSATKELNTAIESIPSNIIANFK
                     KMTVQPMYDLGTEQRQVLDKAPEIKF"
     gene            complement(1000..1392)
                     /locus_tag="PKN22_09065"
     CDS             complement(1000..1392)
                     /locus_tag="PKN22_09065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNS42896.1"
                     /translation="MASIFTKIINKEIPAYIVAENDKFLAFLDIMPLKKGHVLVIPKA
                     EIDYIFDIEDELLSEMMVFAKQVAAKIKKAIPCKKVGVAVVGLEVPHAHIHLIPINSI
                     TDMDFSNPKLQLTEEEFKDVLGLIVNNN"
     gene            complement(1392..1865)
                     /gene="greA"
                     /locus_tag="PKN22_09070"
     CDS             complement(1392..1865)
                     /gene="greA"
                     /locus_tag="PKN22_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNS42897.1"
                     /translation="MAQVSYYTEEGLQKLKDELHEMKTVQRPAISNQIAEARDKGDLS
                     ENAEYDAAKEAQGILEMKIAKLEAIIGSARIIDDANIDTSKVFILSKVKIKNVTNGMT
                     MDYMLVAENEANLKEKKISVESPIGKGLLGKSVGEIADIQTPNGIVKFEIVEIGR"
     gene            complement(1941..>2730)
                     /locus_tag="PKN22_09075"
     CDS             complement(1941..>2730)
                     /locus_tag="PKN22_09075"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNS42898.1"
                     /translation="SLNSAGIAQRLGKVSVIAVSIQSLSYGDIMITTVQNPEGNIGTF
                     SPRANVFNIGYAHTFSNSISGGINIKVISENISNLKSTGVAIDAGIRYVSGEKEQLKF
                     GIALKNVGPTMKYKGDGLNNQVQYLNSSFIGSLEQRTATFEMPSLLSIGGSYDFIFNE
                     MNQLTLGLAFTANSFSYDQFRIGLDYGFSHSKVAFNVRAGYVYEKGIFKAENRANALI
                     GPTCGISVDALVGEKKHALGIEYAARFAGPFGVIHTVGATISLK"
BASE COUNT          883 a          501 c          405 g          941 t
ORIGIN      
        1 tccagatgca aaagcattca acgcattggt ttcaatgatg aataatttag gcattttcat
       61 cccaacagac atacacaagt tttccgtaag attataaaca cgcaggttct cactacgttc
      121 taatggtttg gaatgtgtgg catgttgaat gagtgaagta tgtgcaaaat aagcaataac
      181 aaaccaaata cctacaactc ctaaaacata gggcaaataa gccaaaaaaa gctcgttggt
      241 tgatgttgta tcttcttgcg ttaagaaaaa caaaaccaaa tacacaccca ccaaaatcat
      301 ggcaggaaat gctgcaagca ataaaataga tttttgatta ttacgattta tttgggattg
      361 tagcccaaca tatcttgcca taatctattg ttagaattta atttctggtg ctttgtctaa
      421 aacttgacgt tgttctgtac ccaaatcata cattggttga accgtcattt ttttgaaatt
      481 agcaataata ttagaaggaa ttgattcgat agccgtattc aattctttag ttgcagagtt
      541 aaaataacgt ctaacagcag ctaatttatt ctcaatatca gaaacttcac cttgcaattg
      601 taaaaaatta gcgctggctt ttaaatctgg atacgattca acttgtacat taaatgcttt
      661 cattgctgaa tctagttctt gttctgctgc aattttagaa ttaatatccg ttgcattcat
      721 tgcttttgca cgcatttcgg taactttagt taacacagaa ctttcgtgtt ccatataccc
      781 ttttacggca ccaattaatt gaggaattaa atcatggcgt tgtttcaatt gcacatcaat
      841 gtctgcaaaa gcattttcac ggttgttttt aagcgctact aatttgttgt taagagcaat
      901 agcccaaata actaagacaa cgatgatacc aataactatt aaacctgtca tattattttt
      961 cttttaaaat tagattattt atgatttgtg tgtagcaatt taattattat taacaattaa
     1021 acctaataca tctttaaatt cctcttctgt taattgtaac ttcggattag aaaaatccat
     1081 atcagtaatt gaattaatag gaattaaatg gatgtgtgca tgtggcactt ctaaacctac
     1141 tacagcaacc cctacttttt tacaaggtat agctttttta atttttgcag caacttgttt
     1201 tgcaaacacc atcatttctg atagaagttc atcttcaata tcaaaaatat agtcaatttc
     1261 agcttttggg ataaccaaaa catgtccttt tttaagtggc atgatatcca agaaagctaa
     1321 aaatttatcg ttctcagcaa caatataagc cggaatttct ttattaatta ttttagtgaa
     1381 aatagaagcc attatcgtcc aatttctaca atttcaaatt taactatacc atttggtgtt
     1441 tgaatatctg ctatttctcc tacagattta ccgagcaaac cttttcctat tggcgattca
     1501 acagaaattt tcttttcttt aagatttgct tcattttcag ctactagcat gtaatccata
     1561 gtcatcccat tagtaacatt tttaattttt actttagata gaatgaatac tttagatgta
     1621 tcaatgtttg catcatcgat gatacgtgcg cttccaatta ttgcttctaa ttttgcaatt
     1681 ttcatttcta aaattccttg agcttcttta gctgcatcgt attctgcatt ttcggataaa
     1741 tcacctttat cacgtgcttc agcaatttga ttagaaatag caggtcgttg cacggttttc
     1801 atttcgtgta attcatcttt taatttttgt aatccttcct ctgtgtaata acttacttgt
     1861 gccataacta ctgatttaaa aataaaacaa gagagttcat atagaactcc cttgtcatac
     1921 aaatatataa aattatttac ttacttcaaa ctgattgttg ctccaacagt gtggataaca
     1981 ccaaatggtc ctgcaaaacg tgcagcatat tcaattccaa gcgcatgttt tttctcacca
     2041 accaaagcat caacagaaat accgcaagtt ggtccgatca aagcatttgc tctgttttca
     2101 gctttgaaaa tacctttttc gtaaacataa cctgcacgta cgttgaaagc aactttagaa
     2161 tgtgagaatc cataatccaa accaattctg aattggtcat aagagaatga atttgcagta
     2221 aatgccaaac ctaaagtcaa ttggttcatt tcgttgaaaa tgaaatcata cgatccaccg
     2281 attgataaca atgaaggcat ttcaaatgtt gcagtacgtt gctctaatga acctatgaag
     2341 cttgaattta agtattgaac ttggttattc aaaccatcgc ctttgtactt cattgtagga
     2401 ccaacgtttt tcaaagcgat accgaatttc aactgttctt tttcaccaga aacatagcgg
     2461 ataccagcat cgattgcaac accagttgat ttcaagtttg aaatattctc agaaataact
     2521 ttgatattaa ttcccccaga aatggagtta gagaatgtat gcgcataacc aatattgaac
     2581 acgtttgctc ttggtgagaa agtaccaata ttcccttcag ggttttgaac tgtagtgatc
     2641 atgatatcac catagctcaa tgattgaata cttactgcaa taacagaaac tttacccaaa
     2701 cgttgtgcta taccagcact gtttaatgat
//
LOCUS       DAOQVQ010000326         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44312_length_2726_cov_3.764882, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000326 DAOQVQ010000000
VERSION     DAOQVQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44312_length_2726_cov_3.764882"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKN22_09080"
     CDS             complement(<1..316)
                     /locus_tag="PKN22_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNS42899.1"
                     /translation="MSLELIVDVRSNGIWLALLRDGKLVELHEEQRNIDFAVGDIYLG
                     KVRKVVPSLNAAFVDVGYEKDAFLHYLDLGPQFESLNKFTKDTLKKKQNVADLQYFKI
                     EKE"
     gene            complement(644..934)
                     /locus_tag="PKN22_09085"
     CDS             complement(644..934)
                     /locus_tag="PKN22_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNS42900.1"
                     /translation="MTKADIVASVAEETGLERAEVLKTVEAFMESVRGSLAKGENVYL
                     RGFGSFIVKTRAEKTGRNISKKTTIVIPAHNIPAFKPSKTFVDKVKNNVKVK"
     gene            complement(1134..>2726)
                     /locus_tag="PKN22_09090"
     CDS             complement(1134..>2726)
                     /locus_tag="PKN22_09090"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNS42901.1"
                     /translation="RVFASEIEEDQNPSLETNGILPMLHVFDETARHTTRMHDKKRNH
                     FSVILEPWHADVEAFLDIKKPYIGSGNAMRNLTNVLWIPDLFMHRVKNNEDWTLFSPK
                     ECSELANLYGKEFESAYERFEKMGIGVKTIKAQDLWFKILEAQIETGMPYIMYKDAVN
                     EKSNQKNDGIVKASNYSVNLTLAQADDEIGACSVGAIVLPKFVDSKKEFNFEKLFEVT
                     YQMTLNLNQLLDENYYPVESARDYCQKYRPIGISVVGLADTYALMGIAFDSEAAQAIN
                     RSIFETIYYSALTASKDEAKINGAYSSFKNSPLAKGLFQFNQWNVTPTERWDWETLKA
                     GIAEHGVRNALLVTVSSTESIAQILGYNENIEPFASNISIKRKTSGEYAVINKYLLKD
                     LIAAGLWDKEMRQQITLNSGSIQAVERIPASLKAIYKTVWEIPQKTMLNLAIDRSAFI
                     CQSQAMNVYMENADFSKLTTLHFYAWEKGLKTGMCQLTTKTTVDNLQFSIHRENRVVN
                     TIESEQKVHKNPILTFEHSQSF"
BASE COUNT          784 a          525 c          402 g         1015 t
ORIGIN      
        1 tttccttttc aattttaaaa tattgcaaat cagcaacatt ttgttttttc ttaagggtat
       61 ccttggtaaa tttatttaac gattcgaact gcggacccaa gtctaaataa tgtaaaaaag
      121 cgtctttttc atagcccaca tcaacaaacg cagcgttaag gctaggcact acttttctta
      181 cttttcctag gtatatatcg ccaacagcaa agtcaatatt tctttgctct tcgtgtaatt
      241 caaccagctt tccatcacgt aacagagcaa gccaaatgcc gtttgaacgt acatcgacta
      301 ttaattctaa actcacgaaa gcttaatttt ttattaataa atagaaaaac ttagcaagtt
      361 tatcccgcta agtttccctc aataaatttc cttagaagtt attatttctt ctttttatga
      421 cgattttttc ttaatctttt tttacgcttg tgcgtcgcca ttttatgtct ttttcttttt
      481 ttaccgcttg gcataatctt tatattttaa ttattaattt cttatttctt gtttgtttct
      541 ctttagaaaa aaaacaccct cgtttttcaa cgagagtgca aatttataaa attctattga
      601 aaatcaatca atattatcaa ataaaataat caaaggagag tgattatttt acttttacat
      661 tgtttttcac cttgtcaaca aaagtttttg aaggtttgaa tgcaggaata ttgtgtgctg
      721 gaattacaat tgttgtcttt tttgaaatgt ttcttcctgt tttctcagct cttgttttaa
      781 cgataaagct tccaaatcca cgtaaataaa cgttttctcc cttagccaaa gaccctctaa
      841 ctgactccat aaaagcctca acagtcttta atacttctgc tctttccaac cctgtttctt
      901 ctgcaactga tgcaacgata tctgctttag tcatatttga tataatttaa ttaaagattc
      961 aacaatttga tttccaaata caaagatata cgagatattt atcttcgtag cactaaaagc
     1021 ttattttttt tcacaaatta gcttagttgc agttttttac ccactaattc cccttttttt
     1081 tagacaaata gttgtttttt ctttattaaa ccgttttttt cttagactaa aatttaaaaa
     1141 gattgagaat gttcgaaggt taaaatagga tttttatgca ctttttgctc cgattcaatc
     1201 gtgttaacta ctctgttttc acgatgaata gaaaattgaa ggttatctac cgtggtttta
     1261 gtggttaatt gacacattcc tgttttcaat cctttttccc atgcataaaa atgcaaagtg
     1321 gtcaatttac taaaatctgc attttccata taaacattca tggcttgtga ctgacaaata
     1381 aatgcacttc tgtctattgc caaattgagc atcgtttttt gtggaatttc ccaaactgtt
     1441 ttgtaaattg cctttaaaga tgctggaata cgttctactg cttgaataga accactgttc
     1501 aatgtgattt gttgacgcat ttctttgtcc cacaaccccg cagcaatcaa atctttaagc
     1561 aagtatttgt tgatgaccgc atattcaccc gacgttttgc gttttataga aatattagat
     1621 gcaaaaggtt ctatattttc attgtaaccc aaaatttgtg caatactttc tgttgatgaa
     1681 accgtcacca acaacgcgtt gcgaactcca tgttctgcta ttccagcctt taacgtttcc
     1741 caatcccaac gttctgttgg tgttacattc cattgattaa attgaaacaa acctttggct
     1801 aatggagaat ttttgaatga actgtatgct ccatttattt ttgcttcgtc ttttgatgct
     1861 gttaaagcac tgtaataaat ggtttcaaaa atagaacgat taatagcttg tgccgcttct
     1921 gaatcaaacg caattcccat caatgcgtat gtgtcggcta aaccaacaac gctgatacca
     1981 ataggacggt atttttgaca ataatcgcgt gcactttcca ccggataata gttttcatcc
     2041 aataattgat taaggttcaa cgtcatttga taagtcacct caaacaattt ttcaaaattg
     2101 aattcttttt tgctgtccac aaatttgggt aaaacaatgg ctccaacact gcaagcacca
     2161 atttcatcat ctgcttgcgc taaagttaag ttgacactgt aattggatgc tttaacgata
     2221 ccatcatttt tttgattgct tttttcgttc accgcatctt tatacattat atacggcatg
     2281 cctgtttcaa tttgtgcttc taaaatttta aaccacaaat cttgtgcttt gatggttttt
     2341 acaccgattc ccattttttc aaaacgctca taggcgcttt caaattcttt gccgtataaa
     2401 ttggctaatt cagagcattc tttaggtgaa aaaagtgtcc aatcttcgtt gttttttacg
     2461 cggtgcatga ataaatcggg tatccacaac acattggtaa gattgcgcat cgcatttccg
     2521 ctaccaatat acggtttttt gatgtctaaa aatgcttcta catcggcatg ccaaggttct
     2581 aaaatcaccg aaaagtggtt gcgtttttta tcgtgcatgc gcgtagtgtg acgtgcggtt
     2641 tcatcaaaaa catgcaacat cggaagtatt ccgtttgttt ccaacgatgg attttggtct
     2701 tcttctattt cacttgcaaa aacgcg
//
LOCUS       DAOQVQ010000327         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44593_length_2714_cov_3.586687, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000327 DAOQVQ010000000
VERSION     DAOQVQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44593_length_2714_cov_3.586687"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PKN22_09095"
     CDS             <1..1153
                     /locus_tag="PKN22_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNS42902.1"
                     /translation="PFNNVARTCIEALGAALGGTQSLHTNALDEAIALPTDFSARIAR
                     NTQIYIQRETGITDFIDPTGGSYYIEKLTEELAQQAWELIQEVEELGGMAKAIETGLP
                     KMRIEEAAARKQARIDSGKDIIVGVNQYQVEDNDLLDILEVDNTVVRKAQIDRLNKMR
                     QERDAQKVEAALNALKEAAKTGKGNLLELAVVCARERASLGEISDVMEASFGRFKATI
                     RSISGIYSAEAMDDKDFKKAQEMVEQFTKLEGRRPRIMIAKMGQDGHDRGAKVVATAY
                     ADIGFDVDIGPLFQTPEEAARQAVENDVHILGVSSLAAGHKTLVPQVIAELKKLGRED
                     IMVIAGGVIPQQDYKFLEDAGVFGIYGPGTKVPTAAQDLLRLLIASVSE"
     gene            1241..1969
                     /locus_tag="PKN22_09100"
     CDS             1241..1969
                     /locus_tag="PKN22_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42903.1"
                     /translation="MKNILSTFILLLSLSFSFGQKEYDYLRILYADGSYEKLAKQAEK
                     ITLDDKTKKDPLPYLWLSKALYKIHMSGTADEVFKNAYKESINALGKCIKYDKSGSVF
                     GDEDNKEYLEMIQSTLIEQINNEMLTQNFRKASSWSNTFKKIAQSPIGSMYFEGAAKF
                     RTDDRSSGMALWKTADGLLKDVTSIDTWTKGDKDMLRIGVIQTAECYVSIKKVDLAKN
                     LLNKVAQWFENDTAFKEAYDAIVN"
     gene            2007..>2714
                     /locus_tag="PKN22_09105"
     CDS             2007..>2714
                     /locus_tag="PKN22_09105"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HNS42904.1"
                     /translation="MRYLLFFLLFLIGNQLNAQFTHDDTLRGSITKERVWWDVLHYQI
                     HLQSIDAANKTIAGEVNLKFKVVKENETVMQLDLQQPLSIDNIRFKTTETETKLAFSK
                     VGKSAYWVTLPPTKINQRYELIISYSGSPIEAKNAPWDGGVVWTKDPSNNDFIASACQ
                     GIGASSWWPCKDHQSDEPQDGARIILDVPTHLMGVSNGLLVQDTVLGTIHRTVWEVKN
                     PINNYGINFNVGNYTYWN"
BASE COUNT          923 a          445 c          592 g          754 t
ORIGIN      
        1 tccgtttaac aacgtggcaa gaacgtgtat tgaagccttg ggagcagcac tgggtggaac
       61 acaatcgttg cacaccaacg ctttagatga agccattgca ttgcctacag atttctcggc
      121 aaggattgcc cgtaacacgc aaatttacat ccaacgcgaa acaggaatta ctgattttat
      181 tgacccaact ggtggtagtt attacattga aaaactcacc gaagaattgg cacaacaagc
      241 ctgggaattg attcaagagg tagaagaatt gggtggaatg gccaaagcaa ttgaaaccgg
      301 acttccaaaa atgagaattg aagaagcggc agcacgcaaa caagcccgca tcgattctgg
      361 aaaagatatt attgttggag tgaatcaata tcaagtagaa gacaatgatt tgttggatat
      421 tttggaggta gacaatactg tagttagaaa agcccaaatt gacagactca acaaaatgcg
      481 tcaggagcgc gatgcacaaa aggtagaagc tgcattgaat gcattgaaag aagctgcaaa
      541 aacaggaaaa ggcaatttat tggaattggc ggtagtttgt gcgcgagaac gtgcttcttt
      601 gggcgaaata tccgatgtga tggaagcatc ttttggtcgt ttcaaagcta ccattcgttc
      661 aattagtgga atttattcag ccgaagctat ggatgataaa gattttaaga aagctcaaga
      721 aatggtagag caatttacca agttggaagg acgcagaccg cgtatcatga ttgccaaaat
      781 ggggcaagat ggtcatgata gaggcgcaaa agttgtggca accgcttatg ccgacatagg
      841 ctttgatgta gacattggcc cgttgtttca aacaccagaa gaagctgctc gtcaggcggt
      901 ggaaaacgat gtgcatatat taggagtatc ttcgttggca gctggacaca aaacattggt
      961 tccgcaggtg attgcagaac tcaaaaaact tggtcgcgaa gatattatgg ttattgccgg
     1021 cggtgttatt ccacaacaag attataaatt tttagaagat gcgggtgtgt ttggaattta
     1081 tggacctgga accaaagtac caactgctgc acaagattta ttgcgacttt taattgccag
     1141 tgtttcggag taaaaaattg gaagatgtgt tggaaatagt tttttttact atattcggca
     1201 tggttattga aaattgaaaa ctaaattaaa acaaagaact atgaaaaata tattgagtac
     1261 ttttattttg ttgctttctt tgagtttctc ttttgggcaa aaagaatacg attatttgcg
     1321 tattttgtat gccgatggaa gctatgaaaa gttggcaaaa caagcagaaa aaataaccct
     1381 tgacgataaa acgaagaaag atccacttcc ttatttgtgg ttgtcaaaag cattgtacaa
     1441 aatccacatg tctggaacgg ctgatgaagt atttaaaaat gcctacaaag aatctatcaa
     1501 tgcgttagga aaatgtatta aatacgataa atctggaagt gtttttggcg atgaagacaa
     1561 caaagaatac ttagaaatga ttcaaagtac attgattgaa caaatcaata atgaaatgtt
     1621 gactcaaaac ttcagaaaag catcaagttg gtcaaacaca tttaagaaaa ttgctcaaag
     1681 tccaattggt tctatgtatt ttgaaggagc tgctaaattt agaacagacg accgttcttc
     1741 tggaatggca ttatggaaaa cagcggatgg gttattgaaa gatgtaacat caattgatac
     1801 ttggacaaaa ggagacaaag acatgttgcg cattggtgtt attcaaacgg cagaatgtta
     1861 tgtttctatc aaaaaagtag atttagctaa aaatttatta aataaagtgg cgcagtggtt
     1921 tgaaaatgat actgcattta aagaagcata cgacgcaatt gtaaactgat tttagaaggc
     1981 tgtaaaaaca gccttttttt gtgattatga gatacctttt attttttctt ctatttctta
     2041 ttggcaatca acttaatgca caatttacgc atgacgacac cttgcgcggg agcatcacca
     2101 aagaacgtgt atggtgggat gtgctgcatt accaaataca ccttcaatct attgatgctg
     2161 ccaacaaaac aattgcagga gaggttaacc taaaattcaa agttgttaaa gaaaatgaaa
     2221 ctgtgatgca attagattta caacaacctc taagcataga taacattcgt tttaaaacaa
     2281 cagaaacaga aacaaaactt gctttttcaa aagtagggaa atcagcttat tgggtaactc
     2341 ttccacctac taaaatcaac caacgatacg aacttattat ttcctacagt ggctcaccaa
     2401 ttgaggctaa aaatgcgccg tgggatggtg gtgtagtttg gacaaaagat ccatctaaca
     2461 atgatttcat agcatctgct tgtcaaggaa ttggtgctag ttcttggtgg ccgtgcaaag
     2521 atcatcagtc ggatgaacca caagatgggg cacgaattat tttggatgtt cccacccatt
     2581 taatgggagt ttccaatgga ttattggttc aagacacggt attgggcact atccacagaa
     2641 ctgtttggga ggttaaaaac ccgattaata actatggaat taattttaac gttggcaatt
     2701 acacctactg gaac
//
LOCUS       DAOQVQ010000328         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44660_length_2711_cov_4.136295, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000328 DAOQVQ010000000
VERSION     DAOQVQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44660_length_2711_cov_4.136295"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /gene="secG"
                     /locus_tag="PKN22_09110"
     CDS             complement(<1..249)
                     /gene="secG"
                     /locus_tag="PKN22_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNS42905.1"
                     /translation="MNVLLTILIIIASALLILVVYIQNPKGGGLSSDFGAAHQLGGVQ
                     QTSDFIDKATWSLAGIIAVLSIIMTLRTKSIEPTPQAEK"
     gene            complement(253..1377)
                     /locus_tag="PKN22_09115"
     CDS             complement(253..1377)
                     /locus_tag="PKN22_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42906.1"
                     /translation="MLNLTEIATLIERPELCASSHIESLEKLCVTYPYSQVFPLLYLK
                     ALAQSNDVRLDSELQKYAYRIADRTVLYHLLHQKSIVETEVVVETVAPTPIQPVEIIE
                     EKSIVVEETPKVVETTPEIVQEEKEIVVEAATQSIEPKTELKVEVVPEITHETVEVHT
                     EIENTLAESDIIEQTVEEQTQPEETSEALPSLSDELLQTISGAATYSLEAEEAKLAQQ
                     ALQNKEQEEKEREKQRLIDALIVKKPVEPIVEEETERSFTSWLRKNEAYVEPIQEKPI
                     IADLSEEKPVESTPKPSVKEELFADRTEKKEMYNPIKKAKDSIDDSQLPVSETLAKIF
                     AAQGNFPKAIHIYQQLMLIIPEKKVFFATQIEELTKKLNT"
     gene            complement(1377..1898)
                     /gene="lptE"
                     /locus_tag="PKN22_09120"
     CDS             complement(1377..1898)
                     /gene="lptE"
                     /locus_tag="PKN22_09120"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNS42907.1"
                     /translation="MRKLLAITCLLLGLTSCWPTSVSFNDTGSLNACLTHFQMDVLEV
                     AAPNAPINYPVELTEAVKSGIQNNTKLMLSSVNKQPQVFISGKITSYNVSPIALQEGD
                     NAAKNRLTVSAMFKIVFDCPNENYSHEMNAVSTRFADFDANQDVSSVESQLLSEINSQ
                     IVQDVINQLLSNW"
     gene            complement(1895..>2711)
                     /locus_tag="PKN22_09125"
     CDS             complement(1895..>2711)
                     /locus_tag="PKN22_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS42908.1"
                     /translation="VVAATNENMQKAVASGKFREDLYYRLSTVPISLPPLRERGDDIH
                     LLFRKFASDFSEKYRMPTVRLTMDAVALINHYQWPGNIRQLRNVVEQISIIETERELD
                     AATLQQYLTPIQPKSTMLSTTESMDSINERELMYKFLFDMKKDLTELKKLVVEIINKG
                     DKFAVSGEQVELINRLYNDIEAPLLEPTKLIAPTTPDVLLPQDMMDATQQVDDAYEVH
                     EVVEESLSLEDQEKDLIKKALEKHRGRRKNAAQELGISERTLYRKIKEYNLSE"
BASE COUNT          756 a          522 c          462 g          971 t
ORIGIN      
        1 tttctcagct tgtggagttg gttcaattga ttttgttctc aatgtcatta taatgcttaa
       61 cacagcaatg attcctgcta acgaccaagt agctttatca atgaaatcac ttgtttgttg
      121 aacaccacct aattggtgag ccgctccaaa gtcagagctt aaacctccac cttttggatt
      181 ttgaatatac accaccaaaa tcaataaagc acttgcgata ataataagta tagttaataa
      241 aacattcata atttatgtat ttaatttttt agttaattct tcaatttgag ttgcaaagaa
      301 aacctttttt tctggaataa tcaacattaa ttgttgatag atgtgaattg cctttggaaa
      361 attaccctga gcagcaaaaa tttttgcaag cgtttcacta acagggagct gagaatcgtc
      421 aatgctatcc tttgcttttt ttattggatt gtacatttct tttttctctg ttctatcggc
      481 aaataattct tcttttacag aaggttttgg tgtactttca actggttttt cttctgataa
      541 atccgctata attggcttct cttgaattgg ttcaacatac gcctcgtttt tacgcaacca
      601 cgatgtaaaa cttctctccg tttcttcttc tacaattggt tcaaccggct ttttcactat
      661 taaagcatca attaaacgtt gtttctcgcg ttctttttcc tcttgctctt tattttgtag
      721 cgcttgttgt gccaattttg cttcttctgc ttctaatgaa taggttgcag cacccgatat
      781 ggtttgcaat aattcatccg aaagtgaagg aagtgcttct gaagtttctt ctggttgagt
      841 ttgttcttca acagtttgtt caatgatatc actttctgct agagtattct caatttcagt
      901 atgtacctca actgtttcat gtgtaatttc aggtacaact tctactttta attcagtttt
      961 tggttctatc gattgagtag ctgcttctac aactatttct ttttcttctt gtacaatttc
     1021 tggtgttgtt tctactactt ttggtgtttc ttcaacaact atcgattttt cttcaattat
     1081 ttctaccggt tgaataggtg ttggagcaac tgtttctaca acaacttctg tttcaacaat
     1141 tgatttttga tgcaacaaat gatacagtac ggttctgtca gcaatgcgat aggcgtattt
     1201 ttgcaattca ctatctaaac gcacgtcgtt ggattgcgcc aatgctttta aatagagcaa
     1261 aggaaaaact tgtgaatagg gataagttac acacaatttt tcaagcgatt ctatgtgcga
     1321 tgaagcacac aactctggtc gctctatcaa ggttgctatt tcagttaagt tcaacattac
     1381 caattggaaa gtaattgatt aataacatct tgcacaatct gagaattgat ttcagaaagc
     1441 aattgcgatt caacagaact cacgtcttga ttggcgtcaa aatcagcaaa acgcgtgcta
     1501 actgcattca tttcatgcga ataattttca ttgggacaat caaatacaat tttaaacatt
     1561 gccgaaacag tcaaacggtt tttagcagca ttatcgcctt cttgcaatgc aattgggcta
     1621 acgttgtaac tagtgatttt tccagaaata aaaacttgcg gttgcttgtt aacacttgaa
     1681 agcatcaatt ttgtattgtt ttgaatccct gattttacag cttctgttaa ctcaactgga
     1741 taattaattg gggcattggg cgcagcaacc tccaacacgt ccatttgaaa atgggttaaa
     1801 catgcattta gagaaccagt gtcgttaaag gaaacacttg ttggccaaca cgaagttaag
     1861 cccaacaaca aacaagttat tgcaagtagt tttctcattc agacaagttg tattctttaa
     1921 ttttgcggta caaagtgcgc tccgaaattc ctaattcttg agctgcattt tttcggcgtc
     1981 cgcggtgttt ttccaatgct ttttttatca aatctttttc ttgatcttcc aaagataatg
     2041 attcttcaac aacttcgtgc acttcataag catcgtctac ttgttgtgtt gcatccatca
     2101 tatcttgtgg taacagaaca tctggagtgg ttggtgcaat caatttagtt ggttccaata
     2161 acggcgcttc aatatcatta tacaaacggt tgatgagctc cacttgttca cccgatacgg
     2221 caaatttatc gcctttgttg atgatttcaa ccaccaattt tttcaattcg gttaaatctt
     2281 ttttcatgtc aaacagaaat ttgtacatca actcacgttc attgatgcta tccatacttt
     2341 ctgttgttga caacatagta gattttggtt gaatgggtgt taaatattgt tgaagcgttg
     2401 ccgcatccaa ttcgcgttcg gtttcaatga tagaaatttg ttccaccaca ttgcgcaact
     2461 gacgaatatt tcccggccat tgatagtgat tgataagcgc aacagcatcc atggttaaac
     2521 gaacggttgg catgcgatat ttttctgaaa aatcacttgc aaacttgcga aacaataaat
     2581 gaatatcgtc tccacgttct ctcaaaggcg gtaaactaat gggaacagtg ctcaaacggt
     2641 aatataaatc ctctctaaat ttaccagaag caactgcttt ttgcatattt tcattggttg
     2701 cagctacaac a
//
LOCUS       DAOQVQ010000329         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44741_length_2708_cov_4.554090, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000329 DAOQVQ010000000
VERSION     DAOQVQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44741_length_2708_cov_4.554090"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..>2708)
                     /locus_tag="PKN22_09130"
     CDS             complement(108..>2708)
                     /locus_tag="PKN22_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42909.1"
                     /translation="AIVQNFDENKFIEIDSTQTWDFSTKLYNNLKVKAGTTLTIQCEV
                     QFVPEAKVIIEPGGKLIIDGGKLTNEKYYNNFWQGIQVYGTSNKNQFPESNPTYQGLL
                     VIKNGGIIENAHKAVTNWHQDNWNEIGGVIQATGAVFRNNRRDVEFKSYQNYNTNYTN
                     KYRNLSKFTNTDFITTNDFIEKGLAIQTHVTMWQVDGITFTNCHFSNDITTNKNNSSA
                     PNRGIYSIDAGYSVLAGCSTTPPLGQQCPTANLLKSSFKGFSSAIEATGAATTQTVTI
                     QQANFIDNVWGITIDEFDNVSLNRNTMDVGDADYTLFWPIGAGIMVNNSTGYKIEENT
                     VSTNLAGGFRVGINVSNSGTDDNRVYKNNLQALNNGVAGNGVNHNSNYQKGLQFLCNN
                     FQNNTTAIAINSSPTNDGVRFYQGNFSPLKSAGNTFTNNTTGINNTANSIVYIHNGGN
                     TQPVNNSGMVSLQSTTNTNSCPTSFGGGIIMKPILLVLDSLNTELGGQTASYNNLNFT
                     YTSLIDNGNTEQFKDNIELNWSDDAWKLRGKLLASSPYLSSEVLLKAAKQNVLPNGML
                     LEILLANPDATKGEKFISQLKTVTNNTFPEYMLDYVRGNFDSRTLRTDMEGQLSSIHS
                     ELSNTRSWIKHLTKSKEEYTDNDRLNVVNLGDDIYSKVGVMDYYITQGAFNKADSVLN
                     TINNDKKYTTDLPLIENFSDYINFRENLGNRNLAQLDSTEVAYLQILANGKSRVAGYA
                     QNILCFFYDICYEKELATGNAQNKAMMIPSANSQEALNNVLYTVTLYPNPAKEYTSIQ
                     WEIYDELNNLHYNIVDLNGREQLSGILFDNKGEQVIDTRKLKQGIYIIGIYNNNQLKV
                     SKKLVVENQK"
BASE COUNT          750 a          509 c          466 g          983 t
ORIGIN      
        1 aagaaaatga aatagaaata ttataacaaa aaagcacgct catttgagcg tactttttgc
       61 ttattataga ccttcaaatc caacaacatg gactacaccc tactatatca tttctgattt
      121 tcaacaacca atttcttact cacttttaat tgattgttgt tgtaaattcc aataatataa
      181 ataccttgtt tcaattttcg ggtatcaatg acttgttcgc ctttattgtc gaagagaata
      241 ccgctcaatt gctctctacc gtttaaatcc acaatgttat agtggagatt gtttaactca
      301 tcgtaaattt cccattgaat actagtgtac tcttttgcag gatttggata aagtgtaacc
      361 gtataaagaa cgttatttaa tgcttcttgg ctatttgcac tcggtatcat cattgcttta
      421 ttttgagcgt ttcctgttgc tagctctttt tcataacaaa tatcataaaa gaaacaaaga
      481 atgttttgag catatcctgc cactctgctt tttccatttg ccaagatttg caaataagca
      541 acttccgtgg aatctaattg tgctaaattt ctatttccca aattttcacg gaaattgatg
      601 taatcactaa aattttctat taatggtaaa tctgtcgtgt attttttatc gttattaata
      661 gtgtttaaaa cagaatcggc tttattaaac gcaccttggg taatgtagta atccatgaca
      721 cctactttgc tgtaaatatc atcacctaaa ttgacaacgt taagtctatc attatccgta
      781 tattcttctt tcgactttgt taagtgcttt atccaacttc gtgtattcga caactcggaa
      841 tgaatggagg acaattgccc ttccatgtcg gttcttagtg ttctgctatc aaaatttcct
      901 cttacataat ccaacatata ttcaggaaaa gtattgttgg taacagtttt tagttgactg
      961 atgaattttt caccttttgt ggcatccgga ttggcgagca agatttcaag taacatcccg
     1021 tttggaagta cattttgttt agcagctttt agcaatactt ctgatgaaag gtaaggcgaa
     1081 cttgccaata gctttcctct taacttccaa gcatcatctg accaattcag ttcaatatta
     1141 tctttgaatt gttcagtgtt cccgttgtcg attaaagaag tataagtgaa attcaaatta
     1201 ttatatgagg ctgtttgtcc gcccaattct gtattcaagc tatccaacac cagtagtatt
     1261 ggtttcatga ttattcctcc tccaaaacta gtcgggcatg aatttgtgtt tgtagtggat
     1321 tgaagcgaaa ccatacctga attattaaca ggttgcgtat ttccgccatt gtgaatatag
     1381 acgatggaat tggctgtgtt atttataccc gttgtattgt ttgtaaatgt atttcctgcc
     1441 gattttaaag gcgaaaagtt cccttgatag aagcgtacac catcattggt tggtgaagaa
     1501 ttgatggcta ttgcagttgt attgttttga aagttattgc ataaaaactg taatcctttt
     1561 tgataattgc tattgtggtt cactccatta cctgccaccc cattatttaa cgcttgtaaa
     1621 ttattcttat acacacggtt atcatctgtt ccgctattgc taacattgat acctacccga
     1681 aaaccacctg ccaagttagt ggaaaccgtg ttttcctcaa ttttataacc tgttgaatta
     1741 ttaaccatta tacctgcacc tattggccaa aacagagtgt agtcggcatc tccgacatcc
     1801 atcgtgtttc tgttcaaaga cacattatca aactcatcta tcgttatacc ccaaacattg
     1861 tctataaaat tagcttgctg aatggtaact gtttgggtag ttgccgcgcc agttgcctct
     1921 atggctgagc taaacccttt aaaacttgat tttaacaaat ttgccgttgg acactgttgc
     1981 cctaatggcg gagttgtaga acaaccggct aaaacactgt aacctgcatc tatggaataa
     2041 attcctctgt tgggtgcaga actgttgttt ttattggtag ttatatcatt tgaaaaatgg
     2101 caattggtaa atgttattcc atccacttgc cacatcgtaa catgtgtttg aatagctaag
     2161 cctttttcta tgaagtcatt tgtggtaata aaatcggtat tggtgaattt ggaaagattt
     2221 cggtatttgt tggtgtaatt ggtattgtaa ttttgataac tcttaaactc cacatctcgt
     2281 ctgttgtttc taaaaacagc acctgttgct tgaatgacac cacctatttc attccaatta
     2341 tcctgatgcc aattggttac tgccttgtgc gcgttttcaa tgattccgcc attctttata
     2401 accaataatc cctgataggt tgggttgctt tctgggaatt gattcttgtt ggaagtaccg
     2461 taaacttgaa tgccttgcca gaaattgttg tagtattttt cattggtgag ttttccgcca
     2521 tcaataatga gctttcctcc gggttcgatg ataacttttg cctcgggcac aaattgtact
     2581 tcgcactgta tggttagggt tgtgcctgcc ttgactttta agttgttgta taatttagtg
     2641 ctgaaatccc aagtttgagt tgagtcaatc tcaataaact tattttcatc aaaattttgt
     2701 acaattgc
//
LOCUS       DAOQVQ010000330         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_44932_length_2700_cov_2.989792, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000330 DAOQVQ010000000
VERSION     DAOQVQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44932_length_2700_cov_2.989792"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKN22_09135"
     CDS             <1..216
                     /locus_tag="PKN22_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42910.1"
                     /translation="VSTLYQIKPNETLVFDTIITAQLPLTDRKYTVELVRFKKKRKVK
                     KIRITIPSKLPSTTDSKTWLWRIEKDD"
     gene            256..675
                     /locus_tag="PKN22_09140"
     CDS             256..675
                     /locus_tag="PKN22_09140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNS42911.1"
                     /translation="MKLKQVPLELINSLNKNTLMEALNIVCIELGDDYVKSKMPVNTT
                     THQPAGLLHGGASAALIESIGSMGSVLLIDIEKEVPVGLEINANHIAGVKDGHVIATG
                     KIVHAGKRTHVWQVDIHHEETGKLVCTGRLTVMIVPR"
     gene            679..1626
                     /locus_tag="PKN22_09145"
     CDS             679..1626
                     /locus_tag="PKN22_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNS42912.1"
                     /translation="MQATLKYRIPGQPIVELHGAFCLLDNPKSFEGFLLSDFQAQKFY
                     GFVEGASEPDFQSPQQPIVISEDAYLNLAHEFIHYLQDLNIGKAILSRVKAIPANQTT
                     QNDLFLQLVANYQNAFCYSIEHSHIGNWIGATPEILLSVSNGVGATMSLAGTKPVNDK
                     QAWGEKELHEQQLVTDFIQHIIQENCTNVEVSNRGELVAGPVVHLIHRFQFLVNQQKE
                     WNLITSLHPTPAVSGFPRDKALKCIANFEPHEREFYAGIIGIKSEKQSQLYVNLRCGQ
                     FIENNLYAYVGGGLTQASEPQKEWLETEKKAETLAKFMA"
     gene            complement(1677..1880)
                     /locus_tag="PKN22_09150"
     CDS             complement(1677..1880)
                     /locus_tag="PKN22_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42913.1"
                     /translation="MKKVFTIALVFGALTLTSCKKDWSCECSTGGNAVTTTIKDMTRK
                     DAKEQCDKGDFSGFGVEVECKLK"
     gene            complement(1959..2678)
                     /locus_tag="PKN22_09155"
     CDS             complement(1959..2678)
                     /locus_tag="PKN22_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNS42914.1"
                     /translation="MGNWQTIYKETNGLFDPTVFPLVEAWGFFKKEARIPSSEQIDSI
                     LQFTGFDTNQLHTFHFENDSVYLAKKDPRFQLDFNGIAQGYSVDVVAEFIEKKGHLNY
                     YVEIGGEIRVSGKNSENKNWTIGIDTPKSKDSHHALTTISVSNCGIATSGNYRNFFEQ
                     NGKKYGHILNPKTGYPVQSDIASVTVIAPNATLADAYATYFILLGKEKTIQLFDNIKS
                     IQFILVYSKENSLQIYHSKNL"
BASE COUNT          895 a          460 c          480 g          865 t
ORIGIN      
        1 gtttccactc tttatcagat aaaacccaat gaaacacttg tttttgatac cattatcaca
       61 gctcaattgc ccttaacaga tagaaaatat acggtggaat tggttcgttt taaaaagaaa
      121 agaaaagtta agaaaattag aattaccatc ccatcaaaat tgccttcaac caccgattca
      181 aaaacatggt tgtggcgtat agaaaaagat gattaattgt atatttatgc gaagtaaaac
      241 tcgtattttt gtaccatgaa attaaagcaa gtacctttag aacttatcaa ttccctcaat
      301 aaaaacacat tgatggaagc attgaacatc gtttgcatag aattgggcga tgattatgtt
      361 aaatcaaaaa tgccggtgaa cacaaccacg catcaaccag caggattatt gcacggtgga
      421 gcaagcgctg ctttgataga aagcattggt tcaatgggtt cagtattatt gattgatatt
      481 gaaaaagaag ttcctgttgg acttgaaatc aacgccaatc acattgcagg tgtaaaagat
      541 gggcatgtta ttgcaactgg taaaattgtt catgcaggca agcgcacaca tgtatggcaa
      601 gtggatattc atcacgaaga aactggaaaa ttagtgtgta ctggtcgctt aactgtgatg
      661 attgttccgc gttaagattt gcaagcaact ctaaaatata gaatacctgg tcagccaata
      721 gttgaactcc acggagcttt ttgtttgttg gataacccca aatcgtttga aggtttttta
      781 ctatccgatt ttcaagcaca aaagttttat ggttttgttg aaggtgcaag tgaaccagat
      841 tttcaatcac cccaacaacc aattgttatt tcagaggatg cttatttaaa cttggcacat
      901 gagtttattc actatttgca agatttgaat attggaaaag ccattttatc gcgtgtaaaa
      961 gcaattccag ccaatcaaac cacccaaaat gatttgtttt tgcagcttgt agccaattat
     1021 caaaacgcat tttgttattc tattgagcac tcgcatattg gtaattggat tggagcaaca
     1081 ccagaaatac tattgtcggt gagcaatgga gtaggagcaa ccatgtcact tgcaggaaca
     1141 aaacctgtaa acgataaaca agcttgggga gaaaaagagt tacatgaaca acaattggtt
     1201 accgatttta tacaacacat cattcaagaa aactgcacca acgttgaggt ttcaaatcgt
     1261 ggagaattgg ttgctggccc agtggttcat ctcatacatc gttttcaatt tttagtaaac
     1321 caacaaaaag agtggaattt aataacttca ttgcatccaa ctccagcagt aagcggtttt
     1381 ccaagagata aagcattaaa atgtatcgcc aattttgagc ctcatgagcg cgaattttat
     1441 gctggtataa ttggtataaa atctgaaaaa cagtcacagt tgtatgtaaa tctaagatgt
     1501 ggacaattta ttgaaaataa tctctatgcg tatgtaggtg gcggattaac gcaagcatca
     1561 gagccacaaa aagaatggtt ggaaacagaa aaaaaagcag aaacgttggc taaatttatg
     1621 gcataaaaaa aggagaaaag tttattttct ccttcttcaa taattttggt gataaattat
     1681 ttcaatttac attcaacttc aactccaaaa ccagaaaaat cacctttatc acattgttct
     1741 tttgcatctt tacgagtcat gtctttgatt gttgttgtaa cagcgttacc tcctgttgaa
     1801 cattcgcaag accaatcttt tttacaagat gttaatgtta aagctccaaa tacaagtgca
     1861 atagtaaata cttttttcat gatacttttt tttaaattat tatgcaacaa atgtaataac
     1921 aaataacaat gtagatacta tgtttgcata gttttttttt ataaattttt tgaatgatat
     1981 atttgtaagg aattttcttt agaatagact aaaataaatt gtattgattt aatgttatca
     2041 aacagctgga tggttttttc ttttcccaac aaaatgaaat atgttgcgta tgcatctgct
     2101 agtgttgcat tgggagcaat gactgttacc gaagcaatat ccgattgaac aggataaccc
     2161 gttttgggat tcagaatgtg accatatttc ttgccatttt gttcaaaaaa gttgcgataa
     2221 ttcccactgg ttgctattcc acaattacta acagaaatag ttgttaatgc atgatggcta
     2281 tctttacttt tgggtgtatc aatgcctatg gtccagtttt tattttcact gttttttcca
     2341 ctcactctga tttcacctcc aatttctaca taataattaa gatgtccttt tttctcgatg
     2401 aattcagcta caacatccac cgaataacct tgtgcaatac cattaaaatc aagttgaaaa
     2461 cgagggtctt tttttgctaa atagacgctg tcgttttcaa aatggaatgt atgaagttga
     2521 ttggtgtcaa atcctgtaaa ttgaagaata gaatcaattt gttctgatga tggaatacga
     2581 gcttcttttt tgaaaaaacc ccaagcttca actaacggga aaacggtggg atcgaacaaa
     2641 ccgttggttt ctttgtaaat tgtctgccaa ttccccacac atcaccgatg tttgtcaact
//
LOCUS       DAOQVQ010000331         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45108_length_2693_cov_3.762699, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000331 DAOQVQ010000000
VERSION     DAOQVQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45108_length_2693_cov_3.762699"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKN22_09160"
     CDS             complement(<1..415)
                     /locus_tag="PKN22_09160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNS42915.1"
                     /translation="MKYSLFIFLFISLIVSSCGSSNGGGQGINLFTIEQDKELGAQVA
                     AEIASDPKTYPILDATKYASVYQYVNNIRDKILNAGNVAHRNDFKWEVKIIHNDSTIN
                     AFCTPGGYIYVYTGILKFLDSEDQLAGVLGHEIAHA"
     gene            complement(424..1080)
                     /locus_tag="PKN22_09165"
     CDS             complement(424..1080)
                     /locus_tag="PKN22_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42916.1"
                     /translation="MKSFFRFISYVFIPLAMPLFALLIVFYTPTYIDFKTQINSLYFI
                     EPSFKKFFFNAFALFCWLFPVVTLVVMKLSKQISSIELHHQKERFLPMVMTGAFLVML
                     LIMLYKFNEQLTLSKHLFALVYAGIIASIVYATINFFQKISLHAAGAGLFLGFLFAYY
                     QEQSIAVMWPIYVACLLSGTIVAARMYLQKHSNKELLLGFSIGFVIAFVTDFLLILYF
                     "
     gene            complement(1081..2532)
                     /gene="rpoN"
                     /locus_tag="PKN22_09170"
     CDS             complement(1081..2532)
                     /gene="rpoN"
                     /locus_tag="PKN22_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNS42917.1"
                     /translation="MALKQHLSQKLEQRLSPQQIQLMKLLQVPTMELEQRIKQEIEEN
                     PALEEGKEESDDEYGDNDDDYGDNDSDNENDFDISDYFDDDTPDYKTQVNNRSKDDEE
                     YVVPLSGELTFHQQLTDQLSLVDLDNKQYLIADVLIGNLDDAGYLKRDIDAIVDDLAF
                     SMNVSATEEEVEEVLKVIQELDPAGVGARDLQECLLLQIKRKQDGDITKFTAKKILED
                     HFEEFTKKHYIQIQKKLEIEEEDLKEAIDEIIKLNPKPGGSSKDGSKANQQIVPDFMV
                     TEFEGKLELTINGRNAPELKVSKDYENMLRSYSEGAKTSKSDKEAVQFVKQKLDGAKW
                     FIDAIKQRQNTLLYTMTAIMNYQRDFFLTGDQTQLKPMVLKDIADNVGLDISTISRVA
                     NSKYVQTNYGIFSLKFFFSESLSTDSGEEVSTREVKKILSDAIEGEEKRKPLTDEKLA
                     ELLNEKGYNIARRTIAKYREQLNIPVARLRKEL"
BASE COUNT          845 a          503 c          434 g          911 t
ORIGIN      
        1 cagcatgtgc aatttcgtgt cccaaaaccc ctgctaattg atcttctgaa tctaaaaatt
       61 ttaaaatacc tgtataaaca tagatataac ctcctggtgt gcaaaatgca ttgatggttg
      121 agtcattgtg tatgattttt acttcccatt taaaatcgtt gcggtgcgct acatttccag
      181 cgtttaatat tttatcgcga atattgttta catattggta aaccgatgca tatttagtag
      241 catctaagat gggatacgtt ttaggatcag aggcaatttc tgctgccact tgtgcgccta
      301 attctttgtc ttgttcaata gtaaataggt taataccttg tcctccacca ttgcttgaac
      361 cacaactgct aacaattaat gaaataaaaa gaaaaatgaa taaagagtac ttcatagttt
      421 aaattaaaaa tataaaatta ataaaaaatc tgttacaaat gcaataacaa atccaatact
      481 aaaacctaat aaaagttctt tattagaatg tttttgcagg tacatgcgcg ctgcaacaat
      541 tgttccgctt aataggcaag ccacataaat tggccacata acagcaatgg attgttcttg
      601 ataataggca aaaagaaagc ctaaaaataa gcctgcacct gctgcgtgca aactaatttt
      661 ttgaaagaaa ttaattgtag catacacaat tgatgcaata attccggcat aaaccaatgc
      721 aaataaatgt ttagaaagcg ttaattgttc attgaattta tacagcataa ttaacaacat
      781 taccaaaaaa gcacctgtca ttaccatggg taaaaaacgt tctttttggt ggtgcaattc
      841 aatggagcta atttgtttag aaagcttcat tacaacaagt gtaactactg gaaacagcca
      901 acaaaaaagt gcaaaagcat taaaaaagaa ttttttaaat gaaggctcaa tgaaataaag
      961 cgaattgatt tgtgttttaa aatcaatgta ggttggtgtg taaaaaacaa ttaacaatgc
     1021 aaataaaggc attgccaagg gaataaaaac gtaagaaata aatctaaaaa atgatttcat
     1081 ttacagttct tttctaagtc gtgcaactgg aatgtttaat tgctcacggt atttcgcaat
     1141 ggttcttcga gcaatgttgt atcctttttc attcaacagt tcagccaatt tttcatcggt
     1201 taacggcttt cttttttctt ccccttcaat agcgtctgaa agtatttttt tcacctcgcg
     1261 cgtagaaact tcctctccag aatcagtaga aagcgattca gaaaagaaga atttaagcga
     1321 aaaaatacca taatttgttt gcacatattt gctgtttgcc acacgtgaaa tggtggatat
     1381 atccaatcca acgttgtcgg caatatcttt caaaaccatt ggtttcaact gcgtttgatc
     1441 gccagttaaa aagaaatcac gttggtaatt catgatggct gtcatggtgt aaagcaaggt
     1501 gttttggcgc tgtttgatag catcaataaa ccattttgct ccgtctaatt tttgttttac
     1561 aaattgaacg gcctccttat cagatttact cgttttagcc ccttccgaat agctgcgcaa
     1621 catgttttcg taatctttgc tcacttttaa ttcgggcgca ttcctaccgt tgatggtgag
     1681 ttccaatttc ccttcaaatt cggttaccat aaaatcaggc acaatttgct gattggcttt
     1741 tgaaccatct ttagaagaac ctcctggttt aggattcaac ttaatgattt catcaatggc
     1801 ttctttgagg tcttcttctt caatttctaa ttttttctga atttgaatgt aatgcttttt
     1861 ggtaaattcc tcaaaatggt cttctagtat tttttttgct gtaaatttcg taatatcccc
     1921 atcctgcttg cgctttattt gaagcaacaa gcattcttgc aaatcacggg cacccacgcc
     1981 tgcaggatct aactcttgaa ttacttttaa gacttcctca acttcttctt ctgtagcaga
     2041 aacgttcata gaaaaagcca aatcatcaac aatggcatca atgtcgcgtt ttagataacc
     2101 cgcatcgtct aagttaccta tcaatacatc agcaatgagg tattgtttgt tatccaaatc
     2161 aaccaacgac aattgatcgg ttaattgttg gtgaaacgta agttcacctg aaagtggcac
     2221 tacatattct tcatcgtctt ttgaacggtt gttaacttgc gttttataat cgggtgtatc
     2281 gtcgtcaaaa taatcagaaa tatcaaagtc gttctcatta tccgaatcgt tatctccata
     2341 atcatcgtcg ttatccccat attcgtcatc gctttcttcc tttccttctt ccaacgcagg
     2401 attttcctca atttcttgtt tgatgcgttg ctccaattcc atagttggaa cctgtaacaa
     2461 tttcatcaac tgaatttgct gaggcgataa gcgttgttct aatttttgag aaagatgctg
     2521 ttttaaagcc atgaatattt gattaagtta acacttcaaa aatacggatt tcttttgtta
     2581 taccaacaca ttcaataaaa aaagctaccc ttttgaggta gcttctattc tttttggtaa
     2641 gaaattaatt caggtgaatt aatatttttt tgtcctgcac tttaacaaca tca
//
LOCUS       DAOQVQ010000332         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45364_length_2684_cov_2.952834, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000332 DAOQVQ010000000
VERSION     DAOQVQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45364_length_2684_cov_2.952834"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKN22_09175"
     CDS             <1..326
                     /locus_tag="PKN22_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNS42918.1"
                     /translation="QKLDAPSGTAVTIANAILENNPALNTWKLGEENHPTTQKNELAI
                     TSYRKPDVPGTHIVKYQSSIDTIEIMHEAHNRKGFALGAVLAAEFLKDKKGIFTMSDV
                     LNIGK"
     gene            345..2210
                     /gene="lepB"
                     /locus_tag="PKN22_09180"
     CDS             345..2210
                     /gene="lepB"
                     /locus_tag="PKN22_09180"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNS42919.1"
                     /translation="MSFIYFYLYILIHPYLAMWWKSFELAERKSWEALVPVYNYYVAF
                     KVFNKKGWWAVLLVFPGVHLVMLAVLNVSYIRRFGYFSLLDTVQGIFFPYLIFFKIAK
                     NQDKMLPETNWANSKEMAQREWGDHLVLFLALPVIGHAIALGLGAVTRAKPGSKSKVK
                     EWGDSILFALIAASIIRTYVFEPFKIPTGSMERTLLVGDFLFVNKLAYGPKVPVTPLS
                     HPLTHNTTPFITMKSYTTLEKGSYHRLPGFADVKRYDVVVFNYPAGDTAVYDPRMPYG
                     LMGHEYEGIVNNEAFRLYREESQQRFAVITKFYTDSLQKVGQTSDELLNRLVTERASF
                     EFIRDLNQWKAKARKMIAEDKLTYDGQLGQVIKHHGIIYRPVDKRENYIKRCVGVPKD
                     YLEIRNSILYVNGQPAQVAEHQNLLYIASESNFSTPQSMYERFGIERSDWAIEGGELI
                     GVHANLATIEKVRKAFPDVTFNVYVEPRYTPDANGKFKATEQIENLKTFPKDINVSNN
                     VSNFERFQVPYKGQVVDFTKTNIAYYRRIITAYEGHTLEEKADGIYIDGKKTTTYTVE
                     MNYYWMMGDNRYQSADSRTWGFVPEDHIVGKASMTWYSSNPEYGIRWERVFKAIK"
     gene            2210..>2684
                     /locus_tag="PKN22_09185"
     CDS             2210..>2684
                     /locus_tag="PKN22_09185"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNS42920.1"
                     /translation="MQVFSTAYFGPIAYYAELIQAQQPFVEAKEHFVKQTIRTRCEIL
                     GPNGVQTLSVPVRKVNGNKTAVDALELLDDGWRKMHWKSIETAYSSAAYFDYYGMEIK
                     EILMGNQSTLLELNDAILQRVLMWLDVEKTIGYTSEYQVVEDPKNDFRSVAFDSTS"
BASE COUNT          849 a          474 c          568 g          793 t
ORIGIN      
        1 tgcaaaagtt ggatgcgcca agtggcaccg ctgttacaat tgccaatgct attttggaaa
       61 ataatccagc attgaatacc tggaaattgg gcgaagaaaa ccacccaaca actcaaaaaa
      121 acgaattagc aattacgtcg tatcgcaagc ccgacgtgcc tggaacacac attgtaaaat
      181 accaatcgtc aatagatacc attgaaatta tgcacgaagc acacaaccgc aaaggttttg
      241 cattgggagc tgtgttggct gctgaatttt tgaaagataa aaaaggtatc tttaccatgt
      301 cggatgtact gaatattgga aaataacatt taaaataaaa tcaaatgagc tttatttatt
      361 tctacctcta tattttgatt cacccttatt tagcaatgtg gtggaagagt tttgaattgg
      421 ctgaaagaaa atcgtgggaa gcactcgttc ctgtgtacaa ctactacgtg gctttcaaag
      481 tatttaacaa aaaaggatgg tgggctgtat tattggtatt tccgggtgtt cacttggtga
      541 tgttggctgt attgaacgta agttacatcc gccgttttgg atattttagt ttgttggata
      601 cggtgcaagg tattttcttt ccgtatttga ttttctttaa aattgctaaa aatcaagata
      661 aaatgttgcc cgaaaccaat tgggcaaaca gcaaagaaat ggcacaacgc gaatggggtg
      721 atcatttggt gttgttttta gcattacctg tcattgggca cgcaattgca cttggtttgg
      781 gggctgttac acgtgcaaaa ccgggttcaa aatcgaaagt aaaagaatgg ggcgactcta
      841 ttttgtttgc cttgattgct gcttcaatta ttagaaccta tgtgtttgaa ccattcaaaa
      901 ttccaacagg atccatggaa agaaccttgt tggtgggaga cttcttattt gtcaataaat
      961 tggcttatgg accaaaagtt ccagttacac ctttgtcgca tccattaacg cacaatacaa
     1021 cgcctttcat caccatgaaa tcgtacacaa cgcttgaaaa aggttcgtat caccgtttgc
     1081 ctggttttgc agatgttaaa cgttatgatg tggtggtttt caactatcct gcaggtgata
     1141 ctgcggtgta tgatcctcgt atgccgtatg gtttgatggg acacgaatac gaaggaattg
     1201 tcaacaatga agctttccgc ttgtaccgtg aagaatcaca acaacgtttt gctgttataa
     1261 caaaatttta taccgattct ctacaaaaag ttggtcaaac gagtgatgag ttactcaacc
     1321 gtttggttac agaaagagca agctttgaat ttattcgcga tttgaatcaa tggaaagcta
     1381 aagcacgcaa aatgattgct gaggataagt tgacttatga tggacaattg gggcaagtaa
     1441 ttaaacacca cggaattatc tacagaccag ttgacaaacg tgagaattac atcaaacgct
     1501 gtgttggtgt gcctaaagat tatttggaaa tacgcaattc aattttgtat gtaaacggcc
     1561 aacctgcaca agtggcagaa catcaaaact tattgtacat tgctagtgaa tctaattttt
     1621 ctacaccaca aagcatgtat gagcgttttg gaattgaacg cagcgattgg gccatcgaag
     1681 gaggtgaatt aattggtgtg catgctaatt tggcaactat tgaaaaagtg agaaaagctt
     1741 ttccagatgt tacgttcaat gtatatgttg aacctcgcta tacgcctgat gcaaatggaa
     1801 aattcaaggc tacggaacaa attgaaaatt taaaaacatt cccgaaagac attaatgttt
     1861 caaacaatgt ttccaatttt gaacgttttc aagtgcctta caaaggacaa gtagttgatt
     1921 ttactaaaac aaatattgct tactatcgtc gaattatcac tgcttacgaa gggcatacct
     1981 tggaagaaaa agcagatgga atttacattg atggtaaaaa aacaactacc tataccgtag
     2041 aaatgaatta ctattggatg atgggggata accgttacca atcggcagat tcaagaacgt
     2101 ggggatttgt accagaagat catatcgttg gaaaagcttc tatgacatgg tattcaagca
     2161 acccagaata cggtatccgt tgggagcgtg tgtttaaagc aattaagtaa tgcaggtttt
     2221 ttctacggcc tattttggtc cgattgcata ttacgctgaa ttgattcaag cacaacagcc
     2281 ttttgttgaa gctaaagaac attttgtaaa acaaaccatc cgcacgcgtt gcgaaattct
     2341 cggtccaaac ggtgtgcaaa cattgagcgt accagttcgc aaagttaacg gcaacaaaac
     2401 tgctgttgat gcgttggaat tgttggacga tggttggcga aaaatgcatt ggaaatccat
     2461 tgaaacagcc tattcttctg ccgcttattt tgattattac ggcatggaaa tcaaagaaat
     2521 attgatgggc aaccaatcaa ctttgttaga actcaacgat gccattcttc aacgtgtatt
     2581 gatgtggttg gatgttgaga aaacgattgg atacacctct gaatatcaag tagtagaaga
     2641 ccccaaaaat gattttcgca gcgttgcatt tgattctacc tctt
//
LOCUS       DAOQVQ010000333         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45365_length_2684_cov_2.951693, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000333 DAOQVQ010000000
VERSION     DAOQVQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45365_length_2684_cov_2.951693"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            633..2498
                     /locus_tag="PKN22_09190"
     CDS             633..2498
                     /locus_tag="PKN22_09190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNS42921.1"
                     /translation="MAEVQYTEENIRSLDWKEHIRLRPGMYIGKLGDGSSADDGIYVL
                     VKEVIDNCIDEFVMGNGKKIEVKIKDGKVAIRDYGRGIPLGKVVEVVSKMNTGGKYDS
                     KAFKKSVGLNGVGTKAVNALSSFFKVYSVRDGQKKEVDFDRGNVVKEYELSATDEKNG
                     TYVEFIPDEQIFRKYSFKTAYLNKMMWNYCYLNRELAIHFNGERLQSKNGLKDLLEDN
                     MDGDPLYPIIHLEGEDIEVAFTHGRTYGEDYYSFVNGQNTTQGGTHQSAFRESVAKVI
                     RDFYGKNYEASDIRQSIVAAISVKVIEPVFESQTKTKLGSQEIEPNGKSMRAFVNDFL
                     KEKLDNYLHRNPDIAEAIEKKIKQSERERKELSGIRKIARDRAKKASLHNRKLRDCRI
                     HFTDSKDERADETTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNCYGLTKKIVYE
                     NEEFNLLQAALDIEEEMENLRFNKIVIATDADVDGMHIRMLLLTFFLQFFPDLVKRNH
                     VYVLQTPLFRVRNKKETIYCYSEEERIAAIEKLGRNPEITRFKGLGEISPEEFKNFIG
                     EDMRLEPVVLSKDTTIETILSYYMGKNTQERQDFIIGNLRSDAAIDGLKEELAVD"
BASE COUNT          943 a          444 c          520 g          777 t
ORIGIN      
        1 atgtaacttc tattccttct tcacgaagtg agcgtgctgc ttgagatcct gagtaatcga
       61 attcgcatgc ttgcccgatt acgattggtc cgctacctat gattaaaacg gacttaatag
      121 aattgtcttt tggcatttat gtatgtgttt caatttgatg tttgattctt acaaacaaat
      181 tgagcgcaaa tttaagtcga attggacaat ttacaaagga aaatcgttga ttttttttaa
      241 acatatttta agttattttg tttataactt ctgtttttta attttttata ctattcaaca
      301 taatatcatc cttaaaaaca ccatactcaa cacaataaaa agtaactcaa taagttatgc
      361 atgcttagca tcctctttag ttgcgttaaa aaattaacaa aaaaaaatta aaaaacttgc
      421 attgaataaa attatatacc tatatttgtg ctaggtttag ttgtaaaagg taggttaggt
      481 taggtttgaa aggggtatta cgcaagtatt atccctttca cgttttatac accttcctta
      541 ttcacgttta attgtgaata aatcctcaaa atctttaaaa aaacacgcta atagatgctt
      601 aaatttgtta gctacagaca ttcaacaaca acatggcaga ggttcaatac acagaagaaa
      661 acatacgctc cctcgattgg aaggaacaca tccgcttacg tcctggtatg tatatcggga
      721 aattgggcga tggtagttcg gccgacgatg gtatttacgt tctcgtgaaa gaggttatcg
      781 ataactgtat cgatgaattt gtaatgggaa atggtaaaaa aattgaagtc aaaattaaag
      841 acggaaaagt agcaatacgc gattatggtc gtggaattcc attaggaaaa gtggttgaag
      901 tcgtttcaaa aatgaatacc ggaggtaaat acgactccaa agcatttaaa aaatcggttg
      961 gtttgaacgg tgttggtaca aaagctgtaa acgctctttc cagtttcttt aaagtctatt
     1021 cggttagaga tggacaaaaa aaagaagtcg attttgacag aggaaatgtt gtaaaagaat
     1081 atgaactttc cgctactgac gaaaaaaatg gaacgtatgt ggaatttatt cccgacgaac
     1141 aaatttttag aaaatatagc ttcaaaacag cttatttaaa taaaatgatg tggaattatt
     1201 gctatctcaa tcgagaattg gcaatacatt tcaacggaga acgcctgcaa tctaaaaatg
     1261 gattgaaaga tttgttggaa gacaacatgg acggtgatcc actctacccg attattcacc
     1321 tagaaggcga agatattgaa gtagctttta cacacggacg cacctatggc gaggattact
     1381 attcatttgt gaacggtcaa aacaccacac aaggaggtac acatcaaagt gcttttagag
     1441 agtcagttgc aaaagtgata cgtgattttt atggtaaaaa ctacgaagca tccgatattc
     1501 gtcaatcaat tgttgctgca atttcagtaa aagttattga acctgttttt gaatcgcaaa
     1561 ccaagacaaa attaggttca caagaaattg agcccaacgg aaaatctatg cgtgcttttg
     1621 tcaacgattt tttaaaggaa aagctagaca attacttgca ccgcaatcct gatattgctg
     1681 aagctattga aaagaaaatc aaacaatcgg agcgtgaacg caaagaattg tcgggtattc
     1741 gaaaaattgc acgcgacaga gctaaaaaag cttcattaca caatagaaaa ttgcgcgatt
     1801 gccgtattca ttttacagat tctaaagatg aacgagcaga tgaaaccacc ttgtttatta
     1861 ctgagggaga ctcggcaagt ggttccatta caaaatcacg tgatgtgaat acacaagctg
     1921 tattttcatt gcgtggtaaa ccgctcaact gttatggttt gaccaagaaa atagtgtatg
     1981 aaaacgaaga attcaacctc ttgcaagctg cattggacat tgaagaggag atggagaatt
     2041 tacgtttcaa caagatcgta attgctaccg atgccgatgt ggatggtatg cacatacgca
     2101 tgttgttact aacatttttc ttacaattct tccccgattt agtaaaacgc aaccatgtat
     2161 atgtcttgca aacaccgctt ttccgagtgc gcaataaaaa agaaacaatt tattgctatt
     2221 ccgaagaaga gcgtattgca gctattgaaa aattaggacg aaatccagaa atcacacgat
     2281 ttaaagggtt gggagaaatt tcgccagaag agttcaaaaa ctttatagga gaagacatgc
     2341 gcttagaacc agttgtgttg tcgaaagaca ctaccattga aaccattttg agttattaca
     2401 tgggcaaaaa cacacaagaa cgccaagatt ttatcattgg caacttgcgt tctgacgctg
     2461 caattgatgg tttaaaagaa gagttagcag ttgattaata aagcatctat ttcaaattta
     2521 tgatagaaaa cgacgacgaa ttaaacgaga atttcgataa cgaagaaaac gaagaaacta
     2581 cttccaatgg aggagaaaac accaattttg gtaacctaca ccaacatgaa gaggataccg
     2641 aaggaggttc tatcattcct gttagtggat tatacgaaaa ctgg
//
LOCUS       DAOQVQ010000334         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45388_length_2683_cov_3.578006, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000334 DAOQVQ010000000
VERSION     DAOQVQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45388_length_2683_cov_3.578006"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..2028
                     /locus_tag="PKN22_09195"
     CDS             124..2028
                     /locus_tag="PKN22_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWD domain-containing protein"
                     /protein_id="HNS42922.1"
                     /translation="MNTKLFFAFIASFNLAIAQDVYQTSIKNELVQWDAVRGEWLAES
                     FQAMATNQPIPDRTFPEDLTPAEMYALVPTERRERINTILSQPNETTQNRVPTTDNST
                     INRIPVGSTPNQRIPAAASHDRYTYFVSRPGCELVSGRTYGDPHIRTFDDKSYSFQTV
                     GEYILAASPDRNFEVQTRQKPETDKVSLNTAAAMNVYGDRVGIYASDFPDGQTGTPIR
                     VNGRPVYISNETFYLPHGGTIQNNGREYIVTWPTGEKVQARLSSSGSMRFINVTVYVY
                     RCNGNYFGLMGNANGRSDDDFTGTNNRFNMASSTIFAPFDSRTFGQQETAMEREQLAF
                     LAKDFGRQFLVNDQISLFDYAFGQSSWTFYDPTFPREHITLGDFAQTDRDRARRECER
                     LGVQREDMAGCVLDMAHVNLAPSPRPTQPDRTTGRTITPVTSRQPNVNRPDVFERVPS
                     STGTRTPNTNISNGTIDKTNSGSVERTKNPGTAVEQNSNLRQPAETVRPNTATKHHEA
                     QQVERKPSSTIYNDGYSRKPVGESSVTKNPSVEKPASNSSVERQSTPSRTTSPSTTTT
                     NPTSTPNSGSRPVSTNPVSKPVENTKAQQPSSTVTTPSRTTTTSSPSTGNIKSSGSIN
                     MGKTSSSGGR"
     gene            complement(2126..>2683)
                     /locus_tag="PKN22_09200"
     CDS             complement(2126..>2683)
                     /locus_tag="PKN22_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNS42923.1"
                     /translation="DVKGMKLRVLTCKVSQDEEELIEMVPMKASAGYTSGYADPEYLK
                     VLPTFTLPFLSKDRKYRSFPIKGDSMPPVVEGSHVVAEFVQNWMTIRSGTPCIVVTND
                     DGIVFKIVNNYIEKEQAFQLCSTNTLYQPYMVHVNDILEIWKFVNYISPALPDVQIDN
                     SGISRSIMELQQEVNELKNIITKKG"
BASE COUNT          896 a          551 c          514 g          722 t
ORIGIN      
        1 tgtggaacaa gcagaaaaac actttggtct gaactaagaa atgttaaatt tttgattgaa
       61 aagttaaatt tttaatagtg gcataggttt tgtattttca gttgcacaca aatactgttt
      121 cctatgaata caaaattatt tttcgcattt atcgcatcct tcaaccttgc aatagctcaa
      181 gatgtttatc aaacaagcat caaaaatgag ttggtgcaat gggatgctgt tcgaggtgaa
      241 tggttggccg aaagttttca agcaatggcg actaatcaac ccattccaga tcgtactttt
      301 ccagaggatt taactccagc tgaaatgtat gcattggtac caacagaacg tagagagcgc
      361 atcaatacca tcctttctca acctaacgaa acaacacaaa atcgggtacc aacaacggat
      421 aattcaacca tcaatcgtat tcctgtaggt tcaacaccca accaacgtat tcctgcagct
      481 gcatcgcacg acagatatac gtattttgtt tcacgaccag gatgtgagtt ggtaagtgga
      541 cgtacttatg gcgaccccca cattcgcacg tttgatgata aaagctattc gtttcaaaca
      601 gttggtgagt acattctagc agcatctcct gatagaaact ttgaagtgca aactcgccaa
      661 aaaccagaaa ctgataaagt atcgctcaat actgcagcgg caatgaacgt ttatggcgac
      721 cgtgtgggaa tttatgcaag tgattttcct gacggacaaa caggaacacc tattcgtgtg
      781 aatgggcgac cagtctatat ttccaacgag acgttttatt taccacacgg aggaaccatt
      841 caaaacaatg gtagagaata cattgtaact tggccaactg gcgaaaaggt acaagccaga
      901 ttaagttctt caggtagcat gcgttttatc aatgtaacag tatatgttta tcgatgcaac
      961 ggcaattatt ttggattaat gggcaatgcc aacggaagaa gcgacgatga ttttactgga
     1021 actaacaacc gtttcaatat ggctagctct actatttttg ctccgtttga ttcacgcaca
     1081 tttggtcagc aagaaacagc aatggagcgt gaacaattgg cttttttagc aaaagatttt
     1141 ggacgacaat ttttggtaaa tgatcaaatt tcattgtttg attatgcttt tggacaaagc
     1201 agctggacat tctacgaccc aactttcccg cgtgagcaca ttacattagg agattttgca
     1261 caaacagatc gtgatagagc cagaagagaa tgtgaacgat tgggtgtaca acgtgaagac
     1321 atggcaggat gtgttttaga tatggcgcat gtgaatttag ctccttctcc tcgtccgaca
     1381 caaccggata gaacaacagg aagaacaatt actcctgtaa ctagccgaca accaaacgtt
     1441 aaccgtcctg atgtatttga acgcgttcca agtagcacag gtacacgcac acccaataca
     1501 aacatttcta acggaacaat agataaaaca aacagtggaa gtgtggaacg tactaaaaac
     1561 cctggtacag cagtggagca aaattctaat cttcgtcaac cagcagaaac ggtgcgacca
     1621 aatacagcaa ccaaacacca cgaagcccaa caagtagaga gaaaaccatc atcaactatc
     1681 tacaacgatg gctatagtag aaaacctgtt ggagaaagta gtgtaacaaa aaacccatcg
     1741 gttgaaaaac ctgctagtaa cagttctgtt gaaagacaat ctactccatc gcgaacaaca
     1801 agtccgtcaa caacaaccac taatccaact agtacaccca actcgggctc aagaccagta
     1861 tccaccaatc ctgtttctaa accagttgaa aatacgaaag ctcaacaacc atcttcaaca
     1921 gttacaactc catcaagaac aacaacgact tccagtccat caacaggaaa cataaaaagt
     1981 tcggggagta taaatatggg taaaacaagt agcagtggag gtagataagt agtttaaaaa
     2041 taattgcaaa aaataagtgc cacagatagc agagagaatc attcaatctg tttgtctgtg
     2101 gctttttatt aaaaatagtt gtggattacc cttttttagt aatgatattt tttaattcgt
     2161 taacttcttg ttgcaattcc ataatggaac gagaaatgcc actgttatca atttgcacat
     2221 cgggtaatgc tggagaaatg taattcacaa atttccaaat ttccaaaata tcgttaacgt
     2281 gcaccatata cggttggtaa agtgtattgg ttgaacacaa ttggaatgct tgttcttttt
     2341 caatataatt gttgacaatt ttaaatacaa tgccatcgtc gttagtaacc acaatacaag
     2401 gtgttccaga acgaatagtc atccaatttt gcacaaattc tgccactaca tgagaacctt
     2461 ctacaacagg cggcatagaa tcacctttta ttggaaacga gcggtatttg cggtccttgg
     2521 acaagaaagg caacgtgaaa gttggcaaaa ctttaaggta ttctgggtcg gcatatcctg
     2581 aggtataacc tgctgatgct ttcattggca ccatttcgat caattcttct tcatcttgcg
     2641 acactttaca agtaagtaca cgcaatttca tacctttcac atc
//
LOCUS       DAOQVQ010000335         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45482_length_2679_cov_3.964939, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000335 DAOQVQ010000000
VERSION     DAOQVQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45482_length_2679_cov_3.964939"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1242)
                     /locus_tag="PKN22_09205"
     CDS             complement(58..1242)
                     /locus_tag="PKN22_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNS42924.1"
                     /translation="MSSKRKKNVINIITRISVVGITIITASLVILLSAFNGIEKMIEK
                     LYSEFDAPITIRSAQSKTFFENEIDLQQLKNIPGVKQFSKATEEIVVLKNEDKWANAT
                     LLGTDSVFLELSRTKAHMVDGFPALYENGEPVGLIGATLLDKLGGYIPQEYGRESIVV
                     YGPKRDMKMSLGKNPFNTRQVTIVGRLNYNREVNAEMLIVPIELAQTILGYAENEMSA
                     LLIDVDKKFNKHRVKENIQKAIGEQFIVKTNDEKNELIFQTSKTERLIVIVILLFIFV
                     LASFNLVASLTMLFVEKKEDISILRSLGADDKLLFRIFFYEGLLIAGKGILFGLLLGY
                     AICFAQIQFGLLEMPNSFGEQFPVVTTFFDGFLILFLVSSLSLLASYFPVKMLMKRNS
                     TK"
     gene            complement(1280..1615)
                     /gene="rbfA"
                     /locus_tag="PKN22_09210"
     CDS             complement(1280..1615)
                     /gene="rbfA"
                     /locus_tag="PKN22_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNS42925.1"
                     /translation="MASIRQNKIEGVIQEELSTFFQRNAREVCLGAMVSVTQVRVTSD
                     LSLARCYLSIFAGPDKKEVLKSIQEHATTIRGVLGKRLKNMRKIPEFAYFIDDSLDYA
                     LEIENLLKK"
     gene            1717..>2679
                     /gene="pdxA"
                     /locus_tag="PKN22_09215"
     CDS             1717..>2679
                     /gene="pdxA"
                     /locus_tag="PKN22_09215"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNS42926.1"
                     /translation="MEIDKEKEKETGRDVPVRVGISIGDINGIGPEVIIKALSDHRLF
                     LDCTPIVYASNKILAYHKKLLDNKDFAYQSCKSADEAQNKRVNVVTISDEDVQFELGK
                     ATANGGKFAFLSLEAATQDLAAGKIDVLVTAPISKEAMAMSGFKFPGHTEYLANLAGE
                     SEALMMMVSEDLRVALVTSHIALKDVSAAITKEKVAEKIMQLDKSLRKDFCIQRPKIA
                     VLGLNPHAGENGKMGTEEAEQIIPGIKQAYANGCLAFGPYPADGFFGSSVHHQFDAIL
                     AMYHDQGLTAFKTLAFDNGVNFTAGLPIVRTSPDHGTAFDIVGQN"
BASE COUNT          873 a          514 c          479 g          813 t
ORIGIN      
        1 cgtttctttt ggtgtacgca cttgcatttt attaattttt ttgcaaaaat agtgaaacta
       61 cttagttgaa ttgcgtttca ttaacatttt aacaggaaag taactagcca ataaactgag
      121 cgaacttacc aaaaatagaa ttaaaaagcc atcaaaaaag gtggtgacaa caggaaattg
      181 ctcaccaaaa gaattgggca tttctaacaa accaaactgt atttgagcaa aacaaatggc
      241 atagcccaaa agcaatccaa ataaaattcc cttgccagca atcaacaatc cttcgtaaaa
      301 gaaaatacga aacaataatt tgtcatctgc acccaagctg cgcaaaatag aaatatcttc
      361 ttttttctcc acaaacaaca tggttaacga agcaacaagg ttaaacgatg ccaatacaaa
      421 aatgaacaac aaaatcacaa tcacaatcaa gcgttctgtt ttgctggttt gaaaaatcaa
      481 ttcatttttt tcatcatttg ttttaacgat aaattgctca ccaatagctt tttgaatatt
      541 ttctttgact cggtgtttat tgaatttttt atccacatca atcaataaag cgctcatttc
      601 gttttcagca taccccaaaa tagtttgtgc caattcgatg ggaacaatca acatttctgc
      661 attcacttca cggttatagt tcaagcgccc cactatcgtc acttgacgtg tgttgaatgg
      721 atttttgccc aaactcattt tcatgtcgcg ttttggaccg taaaccacaa tggattctct
      781 accgtattct tgcggaatgt agccccctaa tttatccaat aacgttgcac ctatcaatcc
      841 cactggttca ccgttttcat acaatgccgg aaaaccatct accatgtgag ctttggtgcg
      901 agaaagttct aaaaacaccg aatctgttcc caacaacgtg gcattggccc atttatcttc
      961 gttttttaaa acaacaattt cttcggttgc ttttgaaaat tgcttaactc ccggaatatt
     1021 tttaagttgc tgtaaatcaa tttcgttttc aaaaaaagtt ttggattgtg ccgaacgaat
     1081 ggtaatgggc gcatcaaatt cagaatacaa cttttcaatc attttctcaa tgccgttaaa
     1141 ggccgagagt aaaatcacca aagaagccgt aatgatggtt attccaacaa cagaaatgcg
     1201 tgtaataatg tttattacat ttttcttcct tttggacgac aaataccttc tcgctatgta
     1261 aaaggaaacg tttggcaact tattttttca ataaattctc aatttctagt gcataatcca
     1321 acgaatcatc gatgaaataa gcaaattctg gaattttacg catatttttc aagcgtttac
     1381 ccaaaactcc acgaatagtt gtagcatgtt cttgaatgct ttttaatact tcttttttat
     1441 ctggacccgc aaaaatactg aggtaacaac gtgccaatga taaatcagaa gttactctga
     1501 cttgggtaac actaaccatt gcgccaaggc aaacctcgcg tgcatttctt tgaaaaaaag
     1561 tggataattc ttcttgaatc accccttcaa ttttattttg tcgaattgat gccatagtgc
     1621 aaaggtaaga tttgttattg gattagaaag aattccaact ttattttcaa ttactcaaaa
     1681 gcttgtttat ttttgtaggt gtaaaacata cgatgaatgg aaatcgataa agaaaaagaa
     1741 aaggaaacag gcagagatgt cccggttaga gttggtattt cgattggaga tatcaatgga
     1801 attggcccag aggtgattat caaagcattg agcgatcatc gtttattttt ggattgcaca
     1861 cccattgtgt atgcatccaa taaaatttta gcataccata aaaagttatt agacaataaa
     1921 gattttgctt atcaatcgtg caaatcagct gatgaagcac aaaacaaacg cgttaatgtg
     1981 gttaccatca gcgatgaaga cgtgcagttt gaattaggaa aagcaactgc taatggcgga
     2041 aaatttgcct ttttatcact tgaagcggca acgcaagatt tagctgcagg taaaatcgac
     2101 gtgttggtga cagcacccat ttcaaaagaa gcaatggcaa tgtcgggatt caaattccct
     2161 ggtcataccg aatatttagc caatttagcg ggcgaatcgg aagctttgat gatgatggtg
     2221 tccgaagatt tgcgtgtggc attggtgacc tcacacattg cacttaaaga cgttagcgct
     2281 gccattacca aagaaaaagt ggctgagaaa attatgcaac tcgacaaaag tttgcgtaaa
     2341 gatttttgca ttcaacgacc aaaaattgct gttttgggct taaaccctca cgctggtgaa
     2401 aacggaaaaa tgggtacaga agaagccgaa caaattattc caggaatcaa acaagcttat
     2461 gctaacggat gtttggcatt tggaccttat cctgccgacg gattctttgg ttcatcggtg
     2521 caccatcaat ttgatgctat tttagcaatg tatcacgatc aaggattaac agcctttaaa
     2581 actttggcgt ttgataacgg agtgaatttt actgctggat tacccattgt gagaacatct
     2641 cccgaccatg gaacagcttt tgatattgtg ggacaaaac
//
LOCUS       DAOQVQ010000336         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45943_length_2663_cov_3.232362, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000336 DAOQVQ010000000
VERSION     DAOQVQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45943_length_2663_cov_3.232362"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKN22_09220"
     CDS             complement(<1..520)
                     /locus_tag="PKN22_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42927.1"
                     /translation="MSQDKFQNKYRNGTFRAQWWDYGWNGAYFVTICTQGREHYFGEI
                     KDGKMILSHTGVIAHLLWHEIPKHHTHIELGDFVVMPNHIHGIIIIDKPNNDRIVGQF
                     GHFENDGGCFENGGGCFENDGGHYENGGGCFENGDGCFDNGGGCFENGDGCFDNGGGC
                     FENGDGCFDNGGG"
     gene            complement(631..1584)
                     /locus_tag="PKN22_09225"
     CDS             complement(631..1584)
                     /locus_tag="PKN22_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNS42928.1"
                     /translation="MSKTNYKKAPLTVADQIALLEGRNLTIEDKTKAAAYLNAISYYR
                     LSAYFLPYQQIKDEFNAGVTFKQIINTYSFDRALRLLVFDCIERIEVAIRTQLVYIMS
                     TKYNDSHWQDNQSHFITPYYNKIGNVVDPYSDFQALISKAKTSKTPEVFIKHYVDNYN
                     NPSNPPAWMCFELLTIGELSHIYRGLRNKADKKQIADFFGVHHTVFESWLHSITYVRN
                     ICAHHARLWNKDLAIEPEILLKPQGKWISKPFENNKRIFYFLCMLNYLLQRINTGNTL
                     KNKLDSLFQKYPTIPIQFMGIPSKGGNSTELMDWKNEALWN"
     gene            complement(1752..>2663)
                     /locus_tag="PKN22_09230"
     CDS             complement(1752..>2663)
                     /locus_tag="PKN22_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNS42929.1"
                     /translation="SEDKLPAEKFDYLITNPPFGVKWEKYAKVVREEHESLGHGGRFG
                     AGLPSVSDGSFLFLMHLMSKMKPTDKGGSRLAIVFNGSPLFSGSPSSKKNESSIRQWV
                     IENDLLEAVIALPSQLFYNTGISTYIWVISNHKPKNRKGKVQLINAVEFFKKMSKSLG
                     DKRNELSDEHIAQITQLYGDFEENQYVKVFDNPDFGYSKVTVERPLRDENGNIIKDKK
                     GPKPDASLRDTENIPLKEDISEYMKREVLPHVPDAWIDETKTNIGYEINFTKYFYQYK
                     PLRSLEEIRADILALEQETDGLIKEVI"
BASE COUNT          841 a          512 c          423 g          887 t
ORIGIN      
        1 aaccgccacc gttatcaaaa caaccatcac cgttttcaaa acaaccgcca ccgttatcaa
       61 aacaaccatc accgttttca aaacaaccgc caccgttatc aaaacaacca tcaccgtttt
      121 caaaacaacc gccaccgttt tcataatgac caccatcgtt ttcaaaacaa ccgccaccgt
      181 tttcaaaaca accaccatcg ttttcaaaat gaccgaattg cccgacaata cgatcattat
      241 ttggtttgtc aataattata attccatgga tatgattggg cattacgaca aaatcaccta
      301 attcaatatg cgtgtggtgt ttgggtattt catgccacaa taaatgggca ataacccctg
      361 tgtgggataa aatcatttta ccatctttta tttcaccaaa ataatgttcc ctgccttggg
      421 tacagatggt aacaaaatag gcaccattcc agccataatc ccaccattgt gcgcggaatg
      481 taccgttgcg gtatttattc tggaatttat cttgggacat aaattttaaa ttttaaaaaa
      541 acaatccaaa acaattatac agataaaaat aatgactcgg aatctttcca atccattcaa
      601 tacccgaatt tttgtagtta tcgtattttt ttaattccat aatgcttcgt ttttccagtc
      661 cattaattct gtgctatttc ctcccttact aggtattccc ataaattgta taggtattgt
      721 tggatatttt tgaaataagc tatctaattt gttttttaat gtgtttccag tatttatacg
      781 ctgtaaaaga taattcaaca tgcacaaaaa ataaaaaata cgcttattgt tttcaaatgg
      841 tttagaaatc cattttcctt gaggttttaa caaaatttct ggctcaatgg ccaaatcttt
      901 gttccataat cgggcatgat gtgcacagat gttgcgcaca taggtaatac tatgtagcca
      961 tgattcgaaa acagtgtgat gaactccaaa aaaatcagca atttgttttt tatcagcttt
     1021 gtttctaagt cctctgtata tgtgtgataa ttctccaatg gttaacaatt caaaacacat
     1081 ccaagcaggt ggatttgatg gattgttgta attgtctacg taatgtttta taaatacttc
     1141 tggagttttg gatgtttttg cttttgatat caatgcttga aagtcagaat aaggatcaac
     1201 aacgtttcca attttattgt aataaggtgt aataaaatga gattgattgt cttgccaatg
     1261 agagtcattg tatttggttg acattatgta aactaactgc gttcggatag caacctctat
     1321 gcgctcaata cagtcaaaaa ctaataatct taatgctcta tcaaatgaat atgtattgat
     1381 aatttgttta aaggtaacac ctgcattaaa ttcatcttta atttgttgat aaggtaaaaa
     1441 gtaagcactt aaacgataat agcttattgc attcagatac gccgcggctt ttgttttatc
     1501 ttcaatagtt aaatttcttc cctccaacaa agctatttgg tcagcaacag tgagtggagc
     1561 ttttttgtag tttgttttac tcatatccta gaaagaaaaa accccccaag tgtgcttctg
     1621 tgagaggcat gggaggtgta ttattttgaa ataaaaaaaa tctaactaca agtgttttaa
     1681 cgtacaataa tacaaattgt ttcattaaat ctggtgcaaa gatacaaaga aatttaaaat
     1741 ttaacatacg ttcaaataac ctctttaatt aaaccatccg tttcttgctc caacgccaaa
     1801 atatctgctc taatttcttc caaagaacgc aaaggcttgt attggtaaaa atacttggta
     1861 aagttgattt cgtagccaat atttgttttt gtttcgtcaa tccaagcgtc tggaacatga
     1921 ggaagcactt cacgcttcat gtactctgaa atatcttctt tcaaaggaat gttttcggta
     1981 tctctaaggc ttgcatctgg ttttggacct tttttatctt tgataatgtt tccattttca
     2041 tcgcgtaagg ggcgctcaac ggttactttt gaataaccaa aatcggggtt gtcaaaaact
     2101 ttaacatatt gattttcttc aaaatcacca taaagttgag taatttgtgc aatgtgttca
     2161 tccgaaagtt cattgcgttt gtcgcccaat gatttactca tcttcttaaa aaattcaacg
     2221 gcattgataa gttgcacttt tccttttctg tttttgggct tgtgattgga aatgacccaa
     2281 atataagttg aaatacctgt gttgtaaaac aactggctgg gtagggcaat tactgcttcc
     2341 aacaaatcgt tttcaattac ccattgacgg atactgcttt cgtttttctt agaacttggc
     2401 gaacctgaaa acaacggaga accattaaac acaattgcca aacgcgaacc acctttgtct
     2461 gttggtttca tcttgctcat taagtgcatt aaaaacaaaa atgaaccatc cgaaacagag
     2521 ggcaagccag caccaaaacg accaccatgc ccaagtgatt cgtgttcttc acgcacaacc
     2581 ttggcatatt tttcccattt cacaccaaaa ggcggattgg taattaaata atcaaatttt
     2641 tcagcaggga gtttatcctc aga
//
LOCUS       DAOQVQ010000337         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_45969_length_2662_cov_2.963176, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000337 DAOQVQ010000000
VERSION     DAOQVQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45969_length_2662_cov_2.963176"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..633
                     /locus_tag="PKN22_09235"
     CDS             7..633
                     /locus_tag="PKN22_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNS42930.1"
                     /translation="MALFVSTCGYSQYAYQIAVLKYNGGGDYYANPTSVPNLVKFCNE
                     NLSTKISPDVPFVEVGSPDLFQYPFVHMTGHGNVVFSQQEASNLRNYLQGGGFLHIDD
                     NYGMDQFIRVEIKKVFPNKELVELPFNHPIFHQKYNFNGLPKIHEHDGKRPQAFGIIE
                     NGKVILLYTYETDLGDGWEDINVHQDSPEKHKQALQMGANIINYAFTE"
     gene            834..941
                     /locus_tag="PKN22_09240"
                     /pseudo
     CDS             834..941
                     /locus_tag="PKN22_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            1194..1697
                     /locus_tag="PKN22_09245"
     CDS             1194..1697
                     /locus_tag="PKN22_09245"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HNS42931.1"
                     /translation="MTKTILATSLLFFFISCSNKEIKKTPISSTNETEINLETEEQNT
                     ESFEHEIDKRFDDCINENGCTVCYEECTVKAIKEWRSEMNKYYNLLLENLDEPTKEKL
                     ILSQKKWLEYSEFELQFSNEFYGNMSGTIWGGVGLAQSKEKIKSRALELKAHYDAITG
                     DAVRRPS"
     gene            1930..2259
                     /locus_tag="PKN22_09250"
     CDS             1930..2259
                     /locus_tag="PKN22_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42932.1"
                     /translation="MNVDQLDIDNLIAKGLSFDNDKQRSDDFTIIYRYEGFLWDVNWL
                     KHNRVFAWHVNTSEDLLSKMDRISNMTVDGITQQMEEGNMLKTNESNFYPYRHNESNI
                     FVFLPLY"
     gene            2290..2484
                     /locus_tag="PKN22_09255"
     CDS             2290..2484
                     /locus_tag="PKN22_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42933.1"
                     /translation="MKKFNREIPYNDLPLLPPKADVETKEILRKTISAGRALAQLNGT
                     LLNLPNPTLFLHDLFTRSKS"
     gene            2504..>2662
                     /locus_tag="PKN22_09260"
     CDS             2504..>2662
                     /locus_tag="PKN22_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNS42934.1"
                     /translation="MTTNDELYKSLVADKKVDNSATKEVLSYKEALWLGLEQLKKKPF
                     ITTNLCIGI"
BASE COUNT         1009 a          409 c          466 g          778 t
ORIGIN      
        1 gtatggatag cattgtttgt aagcacctgt ggatattcgc aatatgctta tcaaattgca
       61 gttttgaaat acaatggtgg tggagattat tatgccaacc ctacatcggt tccaaatctt
      121 gtaaaatttt gcaacgaaaa tctatccact aaaatttctc ccgatgttcc atttgtagaa
      181 gtgggtagcc ctgacttatt tcaataccct tttgtgcaca tgacaggaca cggaaacgtt
      241 gttttttcgc aacaagaggc aagtaattta cgcaactatt tgcaaggcgg tggttttttg
      301 cacatagacg acaactacgg tatggatcaa ttcatacgtg tagaaatcaa aaaagtattt
      361 cccaacaaag aattggtaga attgccattt aaccacccta tttttcatca aaaatacaat
      421 ttcaacggat tgccaaaaat tcacgaacat gatggcaagc gaccgcaagc atttggaatt
      481 attgaaaacg gaaaagttat tttgctttac acgtatgaaa ccgacttagg cgatggttgg
      541 gaagatatta acgtgcacca agattctcca gaaaaacata aacaagcgct tcaaatgggt
      601 gccaatatta tcaactacgc atttacggag tagttggttt tagttgaaat aaaacagatt
      661 tgttgcattt attatagata atgaaattgt aagcgttttt caaacaactc tcatacccta
      721 tttcatacca tttacagaaa ctaaaatgga gcactcaaac attaaaacaa acagaatatg
      781 gacagtataa atagaaaaaa tcattgggaa acagtttatg aaacaaaatg aatttaaaaa
      841 acggttttga aaaaattagt tgcttaacag aggatcatac aacaccattt aacaccactc
      901 agaattttct tttttgtagc ttcaaaaaac aacaaaatta agcttgtata ttcactttaa
      961 gaaagaagac aattcacctg ctgtcaaaaa gtgctttctt aaacgaaaat tatcaaaaca
     1021 accgtaaaac aaggtttgat taatgtgaaa taatctgctt aaaaaaaagt ttattgaagt
     1081 tgtttaaatt tgaaataaat gtgtagttta gtgatggaaa ttaacggagg aagctggttt
     1141 aaacgaactg ccgttatgta caattaaaca aaaccgtaaa acagtaaaac attatgacaa
     1201 aaacaattct agcaacatcg ttacttttct tttttatttc atgtagcaat aaagaaatta
     1261 aaaaaacacc tatttcttct acgaatgaaa ctgaaattaa cttagaaacc gaagaacaga
     1321 atacagaatc gtttgaacac gaaatagata aacgttttga tgattgtatt aatgaaaacg
     1381 gatgtactgt ttgttatgaa gaatgtactg taaaagcaat taaagaatgg cgatctgaaa
     1441 tgaataagta ttacaacttg ttgctagaga atttagatga acctactaaa gaaaaattaa
     1501 tcttgtctca aaaaaaatgg ttagaatatt cagaatttga acttcaattt agcaatgaat
     1561 tttacggcaa catgagtgga acaatttggg gtggagttgg tcttgcacag tctaaagaaa
     1621 aaattaaaag cagagcgtta gaattaaagg ctcattatga tgccatcacg ggagatgctg
     1681 taagaagacc aagctaaatc tattctaata aaatagctag caaaatgaca ttattattga
     1741 cgctcgcatt ttgggtttca acaataacgt tttttgctta aactatttca cgtacctcta
     1801 aaccgttagt tgtcagacta aaaaaacgat tataaatatt cgggtggaat tgaacagttt
     1861 agaattgact atgaaattcc actttctgaa atagatcaag aagacgatga gctattttca
     1921 ttaggataaa tgaatgtaga tcaattagat atcgacaatt tgattgctaa aggattaagt
     1981 tttgacaatg acaaacaaag atctgatgat tttacaataa tctataggta tgaaggcttt
     2041 ttgtgggatg taaattggct aaaacataat cgtgtatttg cttggcatgt aaacacctct
     2101 gaagacctac tatcgaaaat ggatagaatt agcaatatga ccgtagatgg aatcacccag
     2161 caaatggaag aaggaaacat gttgaaaaca aacgaaagta atttttaccc ttatcgacac
     2221 aacgagagta atatttttgt atttttaccc ttgtattaga aacaagagta aaacttacac
     2281 ttgaagaaaa tgaaaaaatt caacagagaa ataccgtata atgacctgcc tttgctaccg
     2341 ccaaaagcag atgttgaaac gaaagaaata cttcgtaaaa cgatttcggc aggtagagcg
     2401 ttggctcagt tgaacggaac cttgttaaat ttaccaaatc caactttgtt tttacacgat
     2461 ttatttacaa gaagcaaaag ctagttcgga ggttgaaaac attatcacaa caaacgatga
     2521 actttacaaa tcgttggttg ctgacaaaaa agtggataat tcagccacca aagaagtttt
     2581 gagttacaaa gaggcacttt ggttgggttt ggaacagtta aagaaaaagc catttatcac
     2641 aaccaacctt tgcataggca tt
//
LOCUS       DAOQVQ010000338         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46099_length_2656_cov_3.698962, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000338 DAOQVQ010000000
VERSION     DAOQVQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46099_length_2656_cov_3.698962"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKN22_09265"
     CDS             complement(<1..436)
                     /locus_tag="PKN22_09265"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNS42935.1"
                     /translation="MLHTQTVRPELLELLKNLMEEPLFNDFNLVGGTSLALQIGHRNS
                     IDIDLFGNSPISDEFITFFDTFGNVIINTKSKNIIQLSCNEIKVDFVNYTYPLLEQPL
                     IIENIRLISKKDIAAMKLNAIVGRGSKKDFIDLYFLLEYFSLK"
     gene            complement(391..696)
                     /locus_tag="PKN22_09270"
     CDS             complement(391..696)
                     /locus_tag="PKN22_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42936.1"
                     /translation="MNGAFNISDFSEYLFWDIDVSKFDADKHASQLIQKVVEYGQLSD
                     WNNLLRLYGKERIKETVVNLRSLSPVTHSFLAFYFKIQPEDFRCYTHKQSVQNYWNS"
     gene            complement(711..1634)
                     /locus_tag="PKN22_09275"
     CDS             complement(711..1634)
                     /locus_tag="PKN22_09275"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS42937.1"
                     /translation="MKNILITGAAGFIGKHLCEKLSEKHVLFPFDALTTEASRKRMIF
                     LPNLQTASIHSDFIDKQSVKPDIVIHLAAETGIAGSLNNPALYFHQNVEGTFNVLEQC
                     RKNGVKYLIYASSSSVYEPNEKMPETAPHDKQLSFYGTSKRMTEVMIENYCRQFGMVA
                     IGLRFFTVYGSWTRPDMAAYKFMTAIANGNPITLYNEGNVYRDFTHVSDIVASIELLC
                     EKIVTEPKGWHRVFNIGFGAPVSVQKYAELIAKNLEKELLVEYKSLPSNELTSTHSDT
                     SQLEAYIHYKPKCTIEKGIKEMTDWFKEERF"
     gene            complement(1659..>2656)
                     /locus_tag="PKN22_09280"
     CDS             complement(1659..>2656)
                     /locus_tag="PKN22_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNS42938.1"
                     /translation="GSFLSGGYDSGALVHYMKELKQPPQTFSIGFSKWEKSEDQFAHI
                     VANHLGVDNEAVIADEASLDYLEKMPWVYDEPIADISIVPTFMVSQLARKRVKAVVSG
                     EGADELFGGYTWQHDFYKQSYPTTVLDKLKQKLFKNDTVTFYANAMAMGWFDREELMN
                     MLQPHLHPYIPEDVHWYYRQHFKKELSPLKSIQYLDIKCFMGELVLTKVDRASMANSL
                     EVRVPFLNSSLFSHVFRLKEQVYFKPTQTKYLLYENLKNALPQEILERKKQGFVGPDS
                     YYMNIDWYRNQLKNSTLVELNIVKQSYIDNLLKETYNWKLWKLLVMEKWVRTWLK"
BASE COUNT          834 a          509 c          425 g          888 t
ORIGIN      
        1 ctttcaagct aaaatattct aaaagaaaat ataaatctat aaaatctttt ttgctacccc
       61 ttccaacaat tgcgttcaac ttcattgcag caatgtcttt tttagaaata agtcgaatat
      121 tttctataat caatggttgc tctaacaatg ggtaagtata atttacaaaa tcaactttaa
      181 tttcattaca cgacaattga atgatgtttt ttgattttgt attgatgatt acattaccga
      241 aagtatcaaa aaaagtaata aattcatctg aaataggtga attaccaaat aaatcaatat
      301 caattgaatt tctatgtcca atttgcaaag ctaatgaagt tcctccgaca agattaaaat
      361 cattaaatag aggttcttcc attaaattct ttaagagttc caataattct ggacggactg
      421 tttgtgtgtg taacatcgga aatcttcagg ttgaatttta aaataaaatg ctaaaaaaga
      481 atgcgtaact ggtgaaagtg accttaagtt aacaacagtt tctttaattc tttcttttcc
      541 atagagtcgg agtaaattgt tccaatcaga taattggcca tattcaacta ctttttggat
      601 taattgcgaa gcatgtttat ctgcatcaaa ttttgacaca tctatatccc aaaataagta
      661 ctctgaaaaa tctgaaatat tgaacgctcc attcatatat acaaatgtaa ttaaaatctt
      721 tcttccttaa accaatcagt catttctttg attccctttt caatagtaca cttcggtttg
      781 tagtggatat atgcttctaa ttgtgatgtg tcgctatgtg tggaagttaa ctcattggat
      841 ggaagagatt tgtattcaac caataactct ttttctaaat ttttcgctat caattcggca
      901 tatttttgaa ccgaaacagg agctccaaag ccaatattaa acacgcgatg ccaacccttt
      961 ggttctgtta caattttttc gcacaataat tcaatggaag caacaatatc gctcacgtgt
     1021 gtaaagtctc gatacacatt tccttcgtta tataaagtaa ttgggtttcc gttagcaatt
     1081 gctgtcataa atttataggc agccatatcg gggcgtgtcc acgaaccata aacagtaaaa
     1141 aaacgcaaac caatggctac cataccaaac tgacgacagt aattttctat catcacctct
     1201 gtcattcgtt tgcttgtgcc ataaaacgat agttgtttat catgaggagc tgtttcaggc
     1261 attttttcgt tgggctcata aacagatgag ctactggcat aaatcaggta tttcacaccg
     1321 ttttttcggc attgctccaa cacattaaaa gtgccttcta cattttgatg aaaataaagc
     1381 gcaggattgt tcaacgaccc agcaataccc gtttcggccg ccaaatgaat gacaatatct
     1441 ggttttacgg attgcttatc gatgaaatcg gaatggatgg atgctgtttg taaattgggt
     1501 agaaatatca ttcgttttct agaagcctcg gtagtaagtg catcaaatgg aaacaacacg
     1561 tgtttttccg acaacttttc acaaagatgt tttccaataa atcccgcagc accagtaatt
     1621 agaatgtttt tcattaattg ccgtaaattt tgtttatgct atttcaacca cgttcttacc
     1681 cacttctcca tcaccagtag tttccatagt ttccaattgt atgtttcttt caataaattg
     1741 tcgatatagc tttgtttgac aatgtttaac tcgactaatg tactattttt taattgattt
     1801 ctgtaccaat caatgttcat atagtaacta tcgggaccaa caaaaccttg ttttttgcgc
     1861 tctaaaattt cttgtggcaa ggcatttttc aaattttcat acaataaata cttcgtttga
     1921 gtaggcttga agtaaacctg ttcttttaaa cgaaaaacgt gtgaaaataa tgacgaattg
     1981 agaaatggaa cacgcacttc taacgaattg gccatgcttg ctctatccac tttggtgagt
     2041 accaattcac ccataaaaca tttgatatct aagtattgaa tggattttag aggtgaaagt
     2101 tcttttttaa agtgctgacg ataataccag tgcacatctt ctggaatgta tggatgcaaa
     2161 tgtggttgca acatattcat tagctcttct ctatcaaacc agcccatagc catcgcattg
     2221 gcataaaaag taacggtatc gtttttaaac aatttttgct tgagtttatc caacactgtt
     2281 gtgggataac tttgcttgta aaaatcatgt tgccacgtgt agccaccaaa caattcatcg
     2341 gcgccttcgc cactaacaac ggctttaact cgtttgcgtg caagttgact taccataaac
     2401 gtgggcacaa ttgaaatatc ggcaatgggt tcatcataca cccagggcat tttttccaaa
     2461 tagtctaaac ttgcttcatc ggcaatcacc gcttcgttat ctacgcctaa atgattggct
     2521 acgatgtgcg caaattgatc ctcacttttt tcccacttag aaaaaccaat ggaaaacgtt
     2581 tgtggcggtt gctttaactc cttcatgtaa tgcaccaacg caccactatc ataacctcca
     2641 cttaaaaatg aaccaa
//
LOCUS       DAOQVQ010000339         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46168_length_2654_cov_3.680646, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000339 DAOQVQ010000000
VERSION     DAOQVQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46168_length_2654_cov_3.680646"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="PKN22_09285"
     CDS             complement(<1..2359)
                     /locus_tag="PKN22_09285"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNS42939.1"
                     /translation="MTLVEQSLDKPKKKVAEKTYTHDEVLKKTIAYFKGDELAANVWL
                     NKYAVKDSYGNIYESSPDDMHRRLAAEIARVEKKYPNPLSEDEIYNLLKDFKYIVPQG
                     GPMTGIGNNYQIASLSNCFVIGNQGNADSYGGIMKIDEEQVQLMKRRGGVGHDLSHIR
                     PKSSPVNNSALTSTGVVPFMERYSNSTREVAQDGRRGALMLTISIKHPDSENFIDAKM
                     DGTKVTGANVSVKMDDEFMKAVDSGAPYVQQYPITGVNPKFVKEVNAKALWNKIIHNA
                     WKSAEPGVIFWDTVIRESIPDCYSDLGFKTVSTNPCGEIPLCPYDSCRLLAINLYSYV
                     NNPFTAEASFDFELFKKHSQIALRIMDDIVDLEIEKIDEIIKKIDSDPEMADTKLTEK
                     NLWLKIKDKCLEGRRTGVGITAEGDMFAALGLRYGTDEALNHSVNVHQTLALECYRSS
                     VNLAKDRGAFPIFSAERERNNPFINRIKEVDMDLYENMVKHGRRNIAMLTIAPTGTTS
                     IMTQTTSGIEPVFMVSYKRRRKVNPNDKNVRIDFVDEVGDNWEEYHVFHHKFVTWLEA
                     NGYNMDEVSKMSEENLQLLIEKSPYFKATANDVDWVAKVKMQGAIQKWVDHSISVTVN
                     VPNSVKEELISEIYLTAWKSGCKGVTVYRDGSRSGVLVSDDSKNKKQENIFGETKAPK
                     RPQILEAEIVKFSNDDEKWIAVVGLLNGRPYEIFTGKAEESFEILSKVDSGWVIKNKS
                     EDGKSRYDFQYCDKYGYKTTIEGLSRTFNQEYWNYARLISGILRHG"
BASE COUNT          744 a          555 c          414 g          941 t
ORIGIN      
        1 ttccgtgacg taaaattcca gaaatcaaac gggcatagtt ccaatattct tggttaaacg
       61 ttctagacaa accttcaata gttgttttgt aaccatattt atcgcaatat tggaagtcat
      121 aacgtgattt accgtcttcg ctcttattct tgattaccca gcctgaatca actttagaaa
      181 gaatttcgaa tgattcttct gctttaccag taaagatttc ataagggcga ccattcaata
      241 aacctactac tgcaatccat ttttcatcgt cgttgctaaa tttaacgatt tctgcttcta
      301 agatttgtgg gcgtttagga gcttttgttt caccaaaaat gttttcttgt ttcttgtttt
      361 ttgaatcgtc tgaaaccaaa actcctgagc gtgaaccgtc tctgtaaaca gtcactcctt
      421 tacatccact tttccacgct gttaagtaaa tttcactaat caattcttct tttacagaat
      481 ttggaacgtt caccgtaaca ctaatggaat ggtcaaccca tttttggatg gctccttgca
      541 tttttacttt cgctacccaa tctacgtcgt tggcagttgc tttgaaatac ggtgattttt
      601 cgattaacaa ttgcaagttt tcctcgctca ttttcgaaac ttcatccatg ttgtatccgt
      661 ttgcttccaa ccaagtaaca aatttatggt gaaaaacgtg gtattcttcc cagttgtcgc
      721 ctacctcatc cacaaaatca atgcgcacat ttttatcgtt tggattgact tttcttctgc
      781 gtttgtagga caccatgaac accggctcaa ttccagaagt tgtttgcgtc atgatacttg
      841 ttgtacctgt tggagcaatt gtcaacatcg caatattacg tcttccgtgt tttaccatgt
      901 tttcatacaa atccatatcc acctccttaa tgcggttgat aaatgggttg ttgcgttctc
      961 tttctgcact aaaaattggg aaagcacctc tatcttttgc taagtttaca gaagaacggt
     1021 agcactctaa tgccaatgtt tggtgcacgt ttaccgaatg gttcaacgct tcatctgttc
     1081 cgtaacgcaa gcctaaagca gcaaacatat ctccctctgc tgtaatgccc acacctgttc
     1141 ttcttccttc caagcattta tctttgattt ttaaccaaag gtttttttct gtcaattttg
     1201 tatcggccat ctctggatca gaatcgattt ttttgatgat ttcatcaatt ttttcaattt
     1261 ccaaatcaac aatatcgtcc ataatacgca atgcaatttg tgaatgtttt ttaaacaatt
     1321 caaaatcaaa tgatgcttcg gcagtaaatg gattgtttac atagctgtat aagttgattg
     1381 ccaacaaacg gcagctgtca tacggacaca aaggaatttc tccacatgga ttggtagaaa
     1441 ccgttttgaa gcccaaatca gaatagcaat ctggaataga ttcacgaatt acagtatccc
     1501 agaaaatcac acctggctct gccgatttcc atgcattgtg aatgatttta ttccaaagtg
     1561 cttttgcatt cacttctttt acaaattttg gatttacacc tgtaattgga tattgttgaa
     1621 cataaggcgc acctgaatct accgccttca taaattcatc gtccattttt acagaaacat
     1681 tggcacctgt aacctttgtt ccatccattt ttgcatcaat gaaattttca gaatctgggt
     1741 gtttgataga aattgtcaac atcaacgcac cacgtcttcc atcttgcgcc acttcccgtg
     1801 tagagttgga atagcgctcc ataaatggaa caacacctgt tgatgttaat gcactattgt
     1861 ttaccggtga actttttgga cgaatgtgtg ataaatcgtg accaacacca cctctgcgtt
     1921 tcatcaattg cacttgctct tcgtcaatct tcataattcc accatacgaa tcggcatttc
     1981 cttgattacc aatcacaaag cagttagaca aagaagcaat ttgatagttg tttccaatac
     2041 ctgtcattgg tccgccttgc ggaacaatgt atttaaagtc ttttaataag ttatagattt
     2101 catcctcgct caatggattt ggatattttt tctcaacacg tgcaatttca gccgctaaac
     2161 gacggtgcat atcatctggc gaactttcgt aaatattgcc gtaagaatcc ttcaccgcat
     2221 atttgttcaa ccaaacgttg gcagccaatt catcaccttt gaaatacgca atcgtttttt
     2281 tcaatacttc atcgtgcgtg taggttttct cagccacttt tttctttggc ttatccaagc
     2341 tttgttccac taatgtcata actaattaat ttttcaggtt tataaatgtc ttttcaataa
     2401 tctctttttt tcagtcaaaa aattatgtga ttttgatagg tgcaagttac acacgatttg
     2461 attatttttt tgttcacttt tgaatactta ttaacaccaa ataattcaaa ttcaaatttg
     2521 gaaaattgat ttttttttct tatatcaata ccaaaatttt tgaatttttc gtgaacctac
     2581 gaaatacaag cttttatgaa atttttgatg ccaaaaaatg ataaaaaata aatgtgaaaa
     2641 tgaaattttt gacg
//
LOCUS       DAOQVQ010000340         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46394_length_2646_cov_3.620610, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000340 DAOQVQ010000000
VERSION     DAOQVQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46394_length_2646_cov_3.620610"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKN22_09290"
     CDS             complement(<1..870)
                     /locus_tag="PKN22_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42940.1"
                     /translation="MTSKLFISAFFVGVAAVSYAQPKVVQDAEKMYASQNYCEAADKC
                     ALAYSKLQKKSKSAKKVKADMAFKTAESYRLTERFREATEWYDKAIILEYYNVNPLVY
                     YYNGEMYLALNETDKARKNYEEYKKLVPGDGRADVGLESVAKRDSYVNTLSTKLKVEN
                     QSKINTKSFDMAPMFGDRKEMVMYLGSSRDNSTGTLKDPRTCENYMDIWIAELDKNGN
                     WKEPVLIDGEAINTEDNEGTVAFDGRGKTMFFTRCPNLKKHNLGCEIWVSESKGKNKW
                     GAPKKLDLKPHDSL"
     gene            1136..1771
                     /locus_tag="PKN22_09295"
     CDS             1136..1771
                     /locus_tag="PKN22_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNS42941.1"
                     /translation="MGQSIIDNLYWRYATKKYDASKKVSNEAITIIKEALRLVPTSYG
                     LQPIKFLIIENPEIRKELLHYSYNQFSIVEASHLIVIASLNDIPTQEIDDYIENTATT
                     RSIPIQQLSNYADFLKRTINNLSPTEKSTWAQKQAYIALGVLLDICAQLKIDTTPMEG
                     FDVDGYDKVLKLTEKGLSSTLVIPIGFRHDEDATQHWKKVRKTKEELFEEI"
     gene            1841..>2646
                     /locus_tag="PKN22_09300"
     CDS             1841..>2646
                     /locus_tag="PKN22_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS42942.1"
                     /translation="MKHTIIIVCTLVSFLSIQLGFSQATKTHFDNKAYKEGQFLVQLS
                     NENELKDILKNAPATYQLEIVKYVSPPMRVWLLQFDYNAISHEAMQNWLYQQKGVTVA
                     DYNYYVQVRSTLPGDPSFTSQWHHVNTGQNGGTADADIDSDLAWDITTGGKTATNDDI
                     VVCMVEGSGGNLNHQDLSPNRWTNNYEIPNNNIDDDGNGYIDDYNGWNTSNNTDDTGT
                     GSHGTNCLGMIGAKGDNGLNVVGANWDVKLMVVNMASGMDQANVIEAYTYP"
BASE COUNT          886 a          469 c          411 g          880 t
ORIGIN      
        1 taatgagtca tgtggtttta aatctaattt tttaggtgca ccccatttgt tttttccttt
       61 tgattcagaa acccaaattt cacatcctaa attatgcttt ttcaaatttg gacaacgtgt
      121 aaagaacatt gttttacctc taccatcaaa tgctacagtt ccttcgttat cttctgtatt
      181 gattgcttca ccatcaatta aaacaggttc tttccagtta ccatttttat ccaattcagc
      241 aatccaaata tccatgtagt tttcacatgt acgaggatct ttcaatgtac cagtactatt
      301 atctctactt gaacctaaat acatcaccat ctctttacgg tcaccaaaca ttggtgccat
      361 atcaaatgat ttggtattga tttttgattg gttttctact tttaatttcg ttgacaacgt
      421 attgacataa gaatctcttt tagcaacaga ttctaaacca acatcagcac gaccatctcc
      481 tggaactaac tttttatact cttcgtaatt cttacgcgct ttatccgttt cattcaacgc
      541 caagtacatt tcaccattgt agtaatacac taatggattc acgttgtagt actctaaaat
      601 tattgcttta tcataccact cagtagcttc tctaaaacgt tctgttaaac gataactttc
      661 tgctgtttta aaagccatat ctgctttgac tttttttgca gacttactct tcttttgaag
      721 tttagaatag gctaatgcac atttgtctgc agcttcacaa taattttggc tagcatacat
      781 tttttctgca tcttgaacaa cttttggttg agcataagac acagctgcaa cacctacaaa
      841 aaatgccgag ataaataatt tacttgtcat agaattctgt ttcaatatag taacttttaa
      901 gctaaattaa cttacatctt taaggtttaa cctattattt tttaataaaa agcaaaccaa
      961 aatgaggcta aatatacaaa tattcatatt aaaccaactc ttttttaaaa ttaaaaaaat
     1021 atgaaaattt taacactatt ccatacagtg ctgttttact ggtagttaca acatttttaa
     1081 tactgttttt ttaatccatt atgaaattgt aattttgtga aaaatttaaa ataaaatggg
     1141 tcagtcaatt atagataatc tatattggag gtatgcaact aaaaaatacg atgcttcaaa
     1201 aaaagtatcc aatgaagcca taacaattat caaagaagca ttgcgtttag tgccaacatc
     1261 ttatggtttg caacctataa aatttctgat tattgaaaat cctgaaattc gaaaagaatt
     1321 attgcattat tcatacaatc aattttctat tgtagaagct tctcatttaa tagtaattgc
     1381 atctttgaac gatattccaa cacaagaaat tgacgattat attgaaaata cagcaactac
     1441 gcgttcaatt cctattcaac aattgagtaa ttatgctgat tttctaaaac gcaccatcaa
     1501 caatttaagt ccaaccgaaa aatcaacttg ggcacaaaaa caagcctata ttgcgttggg
     1561 tgtattatta gatatttgtg cgcaactaaa aatagacacc acaccaatgg agggctttga
     1621 tgtggatggc tacgataaag ttttgaaatt aacagaaaaa ggattgagct ccacacttgt
     1681 tattccaatt ggttttcgtc atgatgaaga tgctactcaa cattggaaaa aagtaagaaa
     1741 aacaaaagaa gaactttttg aagaaatcta ataattaaac acttttttta ttttctgttt
     1801 cattttgctt acctttatgt ttcacatata gcatttattt atgaaacata caataatcat
     1861 cgtttgtact ttggtgtctt ttttaagcat tcaattaggc ttttcgcaag caacaaaaac
     1921 acattttgac aataaagcat acaaagaagg gcagtttttg gttcaactct ctaatgaaaa
     1981 tgaattgaaa gatattttaa aaaatgctcc agctacctat caattagaaa ttgtaaaata
     2041 tgtttcccct cccatgcgcg tgtggttgtt acagtttgat tataacgcta tttcacacga
     2101 agcaatgcaa aattggttgt accaacaaaa aggagtaacc gttgccgatt acaattatta
     2161 tgtacaagtg cggtcaactt tgccaggcga tccaagtttt acttctcaat ggcaccatgt
     2221 caacacagga caaaatggag gaacagcaga tgcagacatt gattctgatt tggcgtggga
     2281 tattacaact ggcggaaaaa ctgctactaa cgatgatatt gtggtgtgca tggtagaagg
     2341 ttcgggtggt aatttaaatc atcaagattt gagccccaac cgttggacta acaactatga
     2401 aatacctaac aacaacatcg atgatgatgg caatggctac atagacgatt acaacgggtg
     2461 gaacacttct aacaacactg atgatacagg aacaggttct cacggcacaa attgcttagg
     2521 aatgattggt gctaaaggcg ataacggatt aaacgttgtg ggtgccaatt gggatgtaaa
     2581 attgatggtg gtgaacatgg ctagcggaat ggatcaagcc aatgtaattg aggcttacac
     2641 ttatcc
//
LOCUS       DAOQVQ010000341         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46493_length_2642_cov_4.391187, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000341 DAOQVQ010000000
VERSION     DAOQVQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46493_length_2642_cov_4.391187"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..553
                     /locus_tag="PKN22_09305"
     CDS             125..553
                     /locus_tag="PKN22_09305"
                     /inference="COORDINATES: protein motif:HMM:NF039676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6169 family protein"
                     /protein_id="HNS42943.1"
                     /translation="MYRVAFVVDETFSAISNEDIPNIFQLIIDKASDELEPYDAKVSR
                     TIENIVERFFHNVLNSMIYICSDEDDKAKTRFKVFDRWYKKSECKSFIVKIDNIIQFK
                     ISETETQKMYTSFMFHQDNPTHQKLVEIYYQIEKAQNEEK"
     gene            691..942
                     /locus_tag="PKN22_09310"
     CDS             691..942
                     /locus_tag="PKN22_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42944.1"
                     /translation="MGTAELKLNLHKLIDGVQDNSILEAVYTLLSKANVNTEDDWYNS
                     LPDEIKASIERGISEADNKQYVPYTEVKAKVNKLFRRTT"
     gene            complement(1510..>2642)
                     /locus_tag="PKN22_09315"
     CDS             complement(1510..>2642)
                     /locus_tag="PKN22_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42945.1"
                     /translation="AKNSCGEAVSSATISYKKPVTGSKPTVDITNNCNVTVAHGTYKF
                     SGVVLGVTSTNQIQIKVQNKVVTNAVFTQVANGYNFDVSINAEYGQTYTLEVTATNPF
                     GSATKLCQVTTESKPDNDIVVCVPQGRTRVTMTIKESQWAQYQQMGATLGACPVVDND
                     IVICVPQGNTRVTMTIKESQWAAYEAKGATLGECPVDKDITICLRQGANLTTMTIKES
                     QWAQYQQMGATLGACPVIEQDNDIVICVPQGNTNITMTIKESQWAAYQAKGATLGACQ
                     QQNVNNETDSLSNTIGSNKMIICVVENGVQVTKEINKLEWANYKRKGATEGPCPTSNV
                     IKRPTNVIDGNIKTNTENKVESTKRPITNTTPINRPKSSGGR"
BASE COUNT          849 a          482 c          426 g          885 t
ORIGIN      
        1 tatatgaaag gtaaaacctc aataaatact cttacaaaaa aaggcatcaa acttgcgaac
       61 ccactataat tacgcttttg ataacattac aaatacgtac aattttacaa ccaaaaataa
      121 tattttgtat cgtgtcgctt ttgtagtgga tgaaactttt agtgcaattt caaacgaaga
      181 tattccaaat atttttcagt taataatcga caaagcaagt gatgaacttg agccgtatga
      241 cgcaaaagtt tcaaggacta ttgaaaatat tgtagagcgt ttctttcaca atgttctcaa
      301 ttcaatgatt tacatttgtt cagatgaaga tgacaaagca aaaactcgtt ttaaagtttt
      361 cgacagatgg tataaaaaat ctgaatgcaa atcatttatc gtaaaaattg acaatattat
      421 tcagttcaaa atctctgaaa ctgaaacaca gaaaatgtac acttcattta tgtttcatca
      481 agacaatcca actcatcaaa aattagttga aatttattac caaatagaga aagcacaaaa
      541 cgaagaaaaa taaaaacaag agtaactcaa cacgaaatca cacattttag gaataattat
      601 ttataacaat ttagataaat attccttgtc gagtagttta ttttcatttt tattgtgtta
      661 actattcatt ttttacctta tatttggtat atgggaacag cagaattaaa attaaattta
      721 cacaaattga ttgatggagt gcaagacaat agtattttag aggctgttta tacattactc
      781 tctaaagcta atgtaaatac tgaagatgat tggtacaatt ctcttcctga tgaaataaaa
      841 gcatccattg agcgtggaat ttctgaagct gacaacaagc aatatgttcc ctataccgaa
      901 gtaaaggcaa aagtaaataa actttttagg cgaacaacat gaaggaatcg cactccattt
      961 attggacaat agaagctgaa aaaacctaca tcgatacact tgcttttatt ctaaaacgat
     1021 ggacaatcaa agaagcaaca cactttgaat tactaactga agaattgctt aaacaactct
     1081 ctacaaattt gaaactttgt cctgaaataa aaaaattaaa tattcgaaaa tgtgttgtat
     1141 cagaacaaac ctctctcata tacagaatac aatcacagtc aatagaatta attgcattta
     1201 tagacaatcg aagtcaacat agatattaaa ccacctattc taaaaaacaa aactctattg
     1261 tagaaacttt aatttgattt tttctaaagt gtgtaattgc aaaaagagaa tttaggttca
     1321 atgattctgc ataatttggt taccaccttc ttttacattt cttgtataca aatcggtaat
     1381 tattcacctt gtttatccca aaagatttaa tgtaagtgct tattttctac ctcaataata
     1441 tgtgtagaaa atataaaaaa agctggcaaa acattcattc taccagctct taatcgttca
     1501 acaattttat tacctaccgc cgctactttt aggtctattt ataggagtgg tgttggtaat
     1561 tgggcgtttt gtactttcaa ccttgttttc tgtgttggtt ttaatgttac catctatcac
     1621 attggttggg cgttttatga cgttgcttgt tgggcaagga ccttcagtgg caccttttcg
     1681 tttataattg gcccattcca atttattgat ttctttggtc acctgaacgc cattttcaac
     1741 tacacaaata atcattttgt tggaaccaat ggtgttgctt aatgaatctg tttcgttgtt
     1801 tacattttgt tgctgacaag ctcccaacgt agcacctttt gcttgataag cagcccattg
     1861 cgattctttg atggtcatgg taatgttggt attaccttgt ggaacacaaa ttacgatgtc
     1921 gttatcttgt tctataacag gacaagcgcc caacgttgct cccatttgct ggtattgtgc
     1981 ccattgcgat tctttaatcg tcatggtggt taaatttgcc ccttgacgca aacagatggt
     2041 aatgtcttta tcaactggac attcacccaa cgtagcacct tttgcttcat aagccgccca
     2101 ttgcgattct ttaatggtca tcgttacacg cgtatttcct tgcggaacac aaattacgat
     2161 gtcgttgtca actaccggac aagcacccaa tgttgctccc atttgttggt attgcgccca
     2221 ttgcgattct ttgatggtca tggtaacacg tgtgcgacct tgtggaacac atacaacgat
     2281 atcattatcc ggcttgcttt cggttgttac ctgacacaat ttggttgccg atccaaacgg
     2341 attggttgca gtaacttcca acgtataagt ttgcccgtat tctgcattga tgctaacatc
     2401 aaaattatat ccgttggcaa cttgcgtaaa cacagcgttg gttaccactt tgttttgcac
     2461 ttttatttgt atctgattgg tagatgtaac tccaagcact actcccgaaa atttatatgt
     2521 tccatgcgcc acagttacat tgcaattgtt ggtaatatcc accgttggtt tgctacctgt
     2581 tactggtttt ttataggaaa tagtggcact tgaaacagct tctccacaac tgttttttgc
     2641 ag
//
LOCUS       DAOQVQ010000342         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46550_length_2640_cov_3.976789, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000342 DAOQVQ010000000
VERSION     DAOQVQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46550_length_2640_cov_3.976789"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKN22_09320"
     CDS             <1..463
                     /locus_tag="PKN22_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNS42946.1"
                     /translation="FYSVAVTNNFQQADTGTKMVHLGKNTKSTIISKGISAGKSQNSY
                     RGLVQIHKNAANARNFSQCDSLLMSDTCGAHTFPYIECKNSSAKIEHEATTSKIGEDQ
                     IFYCLQRGISEEKAIGLIVNGYCKEVLNKLPMEFAAEAQKLLAVSLENSVG"
     gene            496..1233
                     /gene="sufC"
                     /locus_tag="PKN22_09325"
     CDS             496..1233
                     /gene="sufC"
                     /locus_tag="PKN22_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNS42947.1"
                     /translation="MIKITNLHASINGKEILKGLNLEVKAGEVHAIMGPNGAGKSTLA
                     SVLAGREDYEITEGSVDFDGTDLLELSTEDRAKEGLFLAFQYPVEIPGVSNINFLRTA
                     LNEIREYRGEEPIPAKDFLAMVKEKSKLVELDGGLASRSINEGFSGGEKKRNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVSNGVNALKTDKNATIVITHYQRLLDYIVPDFVHV
                     LHDGKIVKTGPKELALELEEKGYDWLK"
     gene            1312..2562
                     /gene="sufD"
                     /locus_tag="PKN22_09330"
     CDS             1312..2562
                     /gene="sufD"
                     /locus_tag="PKN22_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNS42948.1"
                     /translation="MEVLENKITKFREGLAQTNLPFRDNLLKTAEETLNTLDFPTTRV
                     ERWKYTRTNKIASTNFFSRKDVVSNLELPLTDAYNLVFVNGYLNTELSSKNFPAGVEI
                     TSIEKSNNEVLGSKVAIETEVFTALNTLYATDGASIRIKAKTVVDKPIQLVHIITGEN
                     TIATLRNVIVSERFSEAEIVQVFLSQNTNASFTNNVTEIHVEEGAKLIIDKLQNEDES
                     NFHYSNESVSQENNTNFKINTITLNGGWVRNDLNIDVDGQNAETHLNGTYVLKGKQHV
                     DNHTVVNHLKPNCHSNEMYKGVMDENSTGVFNGRILVSKDSQKIEAYQSNGNVLLSDN
                     AAINTKPELEIYADDVKCSHGTTTGQLDEEAIYYLRARGISEKSAKALMVTAFISDVL
                     DRMENETVIEYVHQQLNNRFGWEF"
BASE COUNT          926 a          424 c          543 g          747 t
ORIGIN      
        1 attttattct gttgctgtta ccaacaattt ccaacaagca gataccggaa caaaaatggt
       61 gcatttgggt aaaaacacaa agagtacaat tatttccaaa ggtatctcgg caggaaagtc
      121 acaaaactct taccgtgggt tggtgcaaat tcataaaaat gctgccaatg cgcgtaattt
      181 ctctcaatgc gactcgttgt tgatgtctga tacatgcgga gcacacacat tcccatacat
      241 tgaatgcaaa aacagttcag caaaaattga acacgaagca acaacttcca aaattggaga
      301 agaccaaata ttttactgtt tgcaacgtgg tatttcggaa gaaaaagcaa ttggtttgat
      361 tgtgaacgga tattgcaaag aagtattgaa caaacttcca atggaatttg cagccgaagc
      421 acaaaaacta ttggctgtga gccttgaaaa ctcggttgga tagttgaaat taataacgaa
      481 ttagaatttt agaaaatgat caagataaca aatttacatg cctcaattaa tggcaaagag
      541 atattaaaag gattaaacct tgaagtaaaa gcgggagaag ttcacgcaat tatgggaccc
      601 aacggagctg gtaaatctac cctagcatct gttttggcag gtagagaaga ttatgaaatt
      661 accgaaggtt ctgttgattt tgacggtacc gatttattgg agttgagcac agaagacaga
      721 gcaaaagaag gtttgttttt agctttccaa tatccggttg aaattcccgg cgtttcaaac
      781 atcaattttt tgcgcacggc tttgaatgaa atccgtgagt acagaggcga agaaccaatt
      841 cctgctaaag atttcttagc aatggtaaaa gaaaaatcaa aattggttga attagatggt
      901 ggtttggcta gtcgctcgat aaacgaaggt ttttcgggag gcgaaaagaa acgcaacgag
      961 attttccaaa tggctatgtt ggaaccaaaa ttggctattt tggatgaaac cgattctgga
     1021 ttagatattg atgctttgcg cattgtttca aacggggtga atgcattaaa aacagataaa
     1081 aatgcaacca tcgtaattac tcactaccaa cgtttgttgg attacatcgt tccggatttt
     1141 gtacacgtat tgcacgatgg taaaattgtg aaaactggtc caaaagaatt ggcgttggaa
     1201 ttggaagaaa aaggctacga ttggctaaaa taaattgatt tttgcgataa tcgttcaatt
     1261 tattggtttt aagattaatt ttttagaggc ttgcctcaaa caaaatagaa catggaagta
     1321 ttagaaaata aaatcacgaa gtttagagaa ggtttagcac aaaccaacct tccttttcgc
     1381 gataatttat tgaaaactgc ggaagaaaca ttaaatacat tggattttcc aacaactcgt
     1441 gtggagcgtt ggaaatatac acgtaccaac aaaattgctt ctaccaattt cttctcgcgc
     1501 aaggatgtgg tttctaattt agagttgccg ttgacagatg catacaattt ggtatttgtg
     1561 aacgggtatt tgaatacaga actttcatca aaaaacttcc ctgcaggtgt agaaattaca
     1621 tcgattgaaa aatcaaacaa tgaggtgttg ggttcaaaag ttgctataga aacagaagtt
     1681 tttacagcat tgaacacgtt gtatgcaact gatggtgctt ccattcgcat taaagctaaa
     1741 actgttgttg acaaacctat tcaattggtg catattatta caggcgaaaa caccattgca
     1801 acgctaagaa acgtaattgt tagtgagcgt ttttcagaag cagaaattgt gcaagtattt
     1861 ttatcgcaaa ataccaatgc gtcgtttacc aacaacgtta cagaaattca tgttgaagaa
     1921 ggtgcaaagt tgattattga caaattgcaa aacgaagacg aaagtaattt ccactattca
     1981 aacgaaagtg tgagtcaaga aaacaacacg aattttaaaa tcaacaccat tacactaaac
     2041 ggtggttggg ttagaaatga tttaaacatt gatgttgatg gacagaatgc tgaaactcac
     2101 ttaaacggaa catacgtatt gaaaggaaaa caacacgtgg ataaccatac tgttgtaaat
     2161 catttaaaac ctaattgtca ttccaacgaa atgtacaaag gtgtgatgga cgaaaactca
     2221 acaggtgtat tcaacgggcg cattttggtg agcaaagatt cgcaaaaaat tgaagcatac
     2281 caatccaatg gaaatgtgtt gttgtcggat aatgccgcaa taaacaccaa gccagaattg
     2341 gaaatttatg ccgatgacgt aaaatgttct cacggaacaa ctacgggtca gttggatgaa
     2401 gaagccattt attatttgcg tgctcgtgga atttctgaga aatctgcaaa agcgttgatg
     2461 gttacagcat ttatcagcga tgttttggac agaatggaaa acgaaacagt aattgaatac
     2521 gtgcaccaac agttaaacaa tcgttttggt tgggaatttt aagctataat ttcttttatc
     2581 gactaatgta aggcacaagt atattgatca aaatagttta cgtattcgtc ttgtagaata
//
LOCUS       DAOQVQ010000343         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46583_length_2639_cov_3.976393, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000343 DAOQVQ010000000
VERSION     DAOQVQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46583_length_2639_cov_3.976393"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKN22_09335"
     CDS             complement(<1..1030)
                     /locus_tag="PKN22_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS42949.1"
                     /translation="MEIQFQVTPEQAVDEAFLRQKIKTDLGLKTDFNFQWKKRSIDAR
                     KAAIKINCTFEVYVNQEIPAFEPQFQPQNVHHSKEVAIVGAGPAGLYAALRCIELGLK
                     PIVYERGKDVRSRRRDLALLNKELVLNPESNYCFGEGGAGTYSDGKLYTRSKKRGDVL
                     KALKWFVHFGAEYDILVDAHPHIGTNKLPKIITSMREEIEKWGGEVHFETKMTDIFVK
                     NKAIEAIELNHSQVRKFDHVILATGHSARDVFELLHKRDIKIEAKPFALGVRIEHTQE
                     LIDKIQYHGRLNDDFLPPSSYSLVTQVDGRGVYSFCMCPGGIIAPCATAENEVVTNGW
                     SPSKRNNPY"
     gene            complement(1031..1744)
                     /locus_tag="PKN22_09340"
     CDS             complement(1031..1744)
                     /locus_tag="PKN22_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNS42950.1"
                     /translation="MKQLILSLIIFFSFGAIAQKEKPMNYRKFDKRLFHFGFMLGANT
                     SSYTITPKKDAFQTYGLKSLSVNSQPGAQLGILTTLRCGTPMVRLRLLPSLSFQERII
                     HQVFVDPLDITQEIINDERVHSTNIDIPLLFQFRTLRYNNFTAYCMVGGQFTVDLQSQ
                     QNATQKFDDPFIKMKKFDYLGQVGGGIEFFAPFFKFSLEIKYSHSFVNGFIQDNTRVS
                     SPINKIYNSGWVFSLIFEG"
     gene            complement(1816..>2639)
                     /locus_tag="PKN22_09345"
     CDS             complement(1816..>2639)
                     /locus_tag="PKN22_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS42951.1"
                     /translation="LIHLSPEVIVWYRVIIATIGLLIFLPLLKIPIRLANKKDIWKLI
                     LVGIITSAHWVFFYKSIALSTASLGILCLSTTTLHVTWLEPIIMKRKFSWLEFFLGLL
                     IIYGIYFVSSDFEADEYVALAYGLLAALLAAVFSVSNAKLVKTIPSPQITFYEMLTGI
                     FFLTFVLYFDNQLTYEALKMTTTDFWLLLFLGLICTSLAFLLMINVVKVLGAFTASLT
                     INMEPVYTIFMAIIILNEDELLNTNFYIGSFMIIAIVVANTIIKASFSSKKDQNA"
BASE COUNT          872 a          472 c          511 g          784 t
ORIGIN      
        1 aatagggatt gttgcgtttt gaaggcgacc aaccattggt aactacttca ttttctgcag
       61 ttgcacacgg agcaatgatt ccgcccggac acatacaaaa cgaataaaca cctcttccgt
      121 caacttgtgt taccaacgaa taggaggaag gaggcaaaaa atcatcgttt aagcgaccgt
      181 ggtattgaat tttatcaatt aattcttgtg tatgttcaat gcgaacgccc aaggcaaatg
      241 gtttggcttc aatttttata tcgcgtttgt gcaacaattc aaaaacatcg cgcgccgaat
      301 gccctgtggc taatatgaca tggtcaaatt tacgaacttg agaatgattg agttcaatgg
      361 cttcaatggc tttgttttta acaaaaatat cggtcatttt tgtttcaaaa tgcacttcgc
      421 cgccccattt ttcaatttct tcgcgcatgg aagtgataat tttgggcagt ttgttggttc
      481 caatatgcgg atgagcatcg accaaaatat cgtattcggc accaaaatga acaaaccatt
      541 ttaaggcttt tagcacatcg ccacgttttt tggagcgggt gtatagcttt ccgtcagaat
      601 aagttcctgc acctccttct ccaaaacaat agttggattc tggattaagc accaattctt
      661 tgttgagcaa agctaaatca cgtcggcgcg agcgcacatc tttacctctt tcgtaaacaa
      721 taggttttaa acccaattca atgcaacgca atgccgcgta aagtccagca ggaccagcac
      781 caacaatagc aacttctttg gaatggtgta cattctgagg ttgaaattgc ggttcaaacg
      841 ctggaatttc ttggtttaca taaacttcaa acgtacaatt gattttaatt gctgcttttc
      901 gtgcgtcgat ggaacgtttt ttccactgaa aattaaaatc cgtttttaaa cctaagtcgg
      961 tttttatttt ctgacgtaaa aaggcttcgt caaccgcttg ttcgggcgtt acttgaaatt
     1021 gaatttccat ttatccttca aaaattaacg aaaacaccca gccactgttg taaattttgt
     1081 tgattggact agaaacacgt gtgttgtctt gaataaaacc gttcacaaaa ctgtgcgaat
     1141 atttaatttc taagctgaat ttaaagaaag gagcaaaaaa ctcaattccg ccaccaactt
     1201 gtcccaaata atcaaacttt ttcattttga taaaagggtc atcaaatttt tgagtggcat
     1261 tttgttgcga ttgcaaatca actgtaaatt gcccgcccac catgcaataa gctgtaaaat
     1321 tattgtaacg caatgttcta aattgaaaca aaagtggaat atcgatgttg gttgaatgca
     1381 cgcgctcatc gttgatgatt tcttgtgtta tatcaagagg atctacaaaa acttggtgaa
     1441 taatgcgttc ttgaaaagaa agtgaaggca acaaacgcaa acgaaccatt ggtgttccgc
     1501 agcgcaaggt tgttaaaata cccaattgag cgcctggttg tgaattaacc gatagacttt
     1561 tgagtccgta ggtttgaaaa gcatcttttt taggtgtgat ggtgtaacta gaagtgttgg
     1621 cacccagcat aaaaccaaag tgaaacaagc gtttgtcaaa tttccgataa ttcataggtt
     1681 tctccttttg ggctattgca ccaaaggaaa aaaagatgat taacgacaaa attaattgct
     1741 tcattacttt gtattaacgt aaattttcaa aaatagtaca aaaatagact ttagaacgat
     1801 aaaaaaacac gttgcttatg cattttggtc tttttttgaa gaaaaacttg ctttgataat
     1861 ggtgtttgca acaacaattg caataatcat aaacgatccg atgtaaaaat tggtgttgag
     1921 caattcatct tcgttgagaa ttattatagc catgaaaatg gtgtaaacag gctccatgtt
     1981 gatggttagt gaggctgtga atgcacctaa aactttaaca acattaatca ttaataaaaa
     2041 tgcaaggctg gtgcaaatta agcctaaaaa taaaagtaac caaaaatcgg tagtagtcat
     2101 tttgagtgct tcataggtta gctgattatc aaaatacaaa acaaaagtca agaaaaaaat
     2161 tccagtaagc atttcgtaaa atgttatttg aggcgaagga attgttttta ctaatttggc
     2221 attggaaaca gaaaagacgg cggccaacaa agcagccaac aagccataag caagcgccac
     2281 atattcatct gcttcaaaat cggatgaaac aaaataaatt ccataaatga tgagcagtcc
     2341 gaggaagaat tccagccacg aaaatttgcg tttcatgatg atgggttcta accaagtaac
     2401 gtgtaaagtg gtggtggaaa ggcataaaat tcctaaagat gccgttgata gtgcaataga
     2461 tttatagaaa aacacccaat gggcgcttgt aataatgcct actagaatta atttccagat
     2521 gtctttttta ttggcaagac gaattggaat ttttagtagc ggtaaaaaaa tgagcaatcc
     2581 aattgtggca ataataacgc ggtaccacac aattacttcg ggagaaagat ggattaatt
//
LOCUS       DAOQVQ010000344         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46817_length_2631_cov_3.496506, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000344 DAOQVQ010000000
VERSION     DAOQVQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46817_length_2631_cov_3.496506"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKN22_09350"
     CDS             <1..866
                     /locus_tag="PKN22_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42952.1"
                     /translation="PLVMRKLWNQSGGTQGAFVVATSASWGIDYGNPASYPLWCQFYD
                     TLGYHGILNIGATTNSNINVDINGDMPTACSSDYMIGVGRTDNKDNTAGGYGVTKIPL
                     GAPGINVVTTSGTSGITTTTGTSFACPLTAGVVGLAYSIPCAHFMSIVKDNPRQGADL
                     VRAALLNGVDVKTQLQSKFSTGGRLNAKNTLDSLMQSACLAGLETTSNEKLVVYPNPA
                     QDYIHFSFGETPQQATLVMRDLVGRIIATYTIATSTLQIPVSNYAKGTYLYEIRTNTQ
                     LVLNSGKVVVE"
     gene            888..1706
                     /locus_tag="PKN22_09355"
                     /pseudo
     CDS             888..1706
                     /locus_tag="PKN22_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            1932..>2631
                     /locus_tag="PKN22_09360"
     CDS             1932..>2631
                     /locus_tag="PKN22_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNS42953.1"
                     /translation="MLGIIVLISASVYYFKDEIIGIVLEEVNAHLKAKVKVEKVDLAF
                     WSSFPNLSVDFNKVFIKDSYEEATEKDTLFYSDKIRLKFNPLDIWRENYKLKQIEVLP
                     GTLQLKIDKNGKNNFDIFKETADTVETAFAFDLQKLYINNLRFSYDDKVINQRYATDL
                     LTTELEGKFTEKVFTMHAVSSQIVRETRSGQINFVSNKKAKININIEINREKGTIIIP
                     KTNVYLANLPFQFSG"
BASE COUNT          924 a          426 c          485 g          796 t
ORIGIN      
        1 atccattagt aatgcgtaaa ttgtggaacc aatcgggtgg aactcaaggt gcatttgtgg
       61 ttgcaacaag tgcttcttgg ggaattgatt atggaaaccc cgctagttat ccgctttggt
      121 gccaatttta cgacacattg ggttatcacg gaattttaaa cattggagca actaccaatt
      181 ctaatattaa tgtggatatc aatggcgaca tgccaaccgc ttgttcaagt gattatatga
      241 taggagttgg tagaacagat aacaaagaca acactgctgg tggatatggt gttacaaaaa
      301 ttccattggg tgcaccgggc atcaacgtgg ttacgacctc tggaacgtcg ggaattacaa
      361 caactacagg tacttctttt gcttgcccgc ttacggctgg tgttgttggt ttggcctact
      421 ccattccgtg cgctcatttt atgtcgattg taaaagataa tccacgtcaa ggtgcggatt
      481 tagtgagagc agccttgcta aatggcgtag atgttaaaac ccaattgcaa agcaaatttt
      541 caacaggcgg aaggttgaat gctaaaaaca ccttagacag tttgatgcaa agtgcgtgtt
      601 tggctggttt agaaacaacc tccaatgaaa agttggtggt gtatccaaat cctgcacaag
      661 attatattca tttttcgttt ggtgaaactc cacagcaagc aacacttgtt atgcgcgatt
      721 tggttggacg aattattgca acctacacaa ttgcaacttc tacactccaa attccagttt
      781 ctaattatgc aaaaggaacg tatttgtatg aaattcgaac caacacacaa ttggttttga
      841 atagcggaaa agtggttgta gagtaaaaaa tcatacgtat aatcaaaatg tctaaacgta
      901 gaattataca agtaaaagaa tcgtcaatta aggttataaa tcataatgat actgattata
      961 taagtttaac tgatatgact gggggattta gagaaggaag tggattaatt ggaaaatgga
     1021 ttacaaataa aaacacactt gaataccttg gtgtatggga aagttaaaaa aacgattatt
     1081 ttaattactc cgaattcgag gtaattaaaa atgaatccgg tgttaataga tttgtaatgt
     1141 ctgttggtca atggattgaa cggactaaag caataggtat gactgtatca actggtcggt
     1201 atggaagtac atttgctcac aaggatattg cttttcattt tgctatgtgg cttagtccag
     1261 aatttcaaat atacttaatt aatgaatttc aacgtttaaa agatgaagaa caaaagaaca
     1321 gtaacctaga gtggaatcta caacggactc taacaaaagt taattatttt attcatactg
     1381 attccataaa agagaactta attcctttag aaatttccaa aacacaagct agcctaattt
     1441 atgcgagtga ggcagattta ttaaatgttg ccctttttgg aataacagcg aatgaatgga
     1501 gagaaaaaaa tccaacctta aaaggaaata tcagagatta ttctacactt gagcaacttg
     1561 ttgtattaag taatttagaa agtatcaacg ctttacttat aaaacaaaaa atcacacaaa
     1621 aagagcggtt aattcaatta aataaagttg caataacaca aatgaagtct ttggttaaac
     1681 atcgtgcttt aagacaatta gaataactaa taaatacatc aactttagca aaaagttaat
     1741 gacgacaatg aatttcattt acgctccttt tggagcgttt tttgtttagt gcctcttgtt
     1801 aaaataaaag caacaattag gcgttaataa aattcaaaac tccctctccc tctcacttta
     1861 attatcttct atttttgttt acaatgaata aaaagcgtgt atttaaaatt ctaaaatggt
     1921 tttttggcat tttgctgggc ataattgtat tgatttctgc cagtgtgtat tatttcaaag
     1981 acgaaattat tggaattgta ctcgaagaag taaacgcaca cttaaaagca aaagtgaaag
     2041 tagaaaaagt agatttggct ttttggagca gttttcctaa tctttctgtt gactttaaca
     2101 aggtatttat caaagattca tacgaagagg ctacagaaaa agacaccttg ttttattccg
     2161 acaaaattcg attaaaattc aatccattgg atatttggcg agaaaattat aaactcaaac
     2221 aaattgaagt tcttccggga actttgcaac ttaaaattga taaaaacgga aaaaacaatt
     2281 ttgatatttt taaagaaaca gctgatacgg ttgaaacagc atttgctttc gacttgcaaa
     2341 aattatacat caacaacctc cgtttttcgt atgacgacaa agtcatcaac caacgttatg
     2401 ccaccgattt gttgaccacc gaattggaag ggaaatttac agaaaaggtg tttaccatgc
     2461 atgccgtcag ttcacaaatt gtgcgcgaaa caagatcggg acaaatcaat tttgtttcca
     2521 acaaaaaggc gaaaatcaac attaacattg aaattaaccg tgaaaaaggc accatcataa
     2581 ttccaaaaac aaacgtgtat cttgccaatt taccttttca attttcggga a
//
LOCUS       DAOQVQ010000345         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_46867_length_2629_cov_4.027972, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000345 DAOQVQ010000000
VERSION     DAOQVQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46867_length_2629_cov_4.027972"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PKN22_09365"
     CDS             <1..1315
                     /locus_tag="PKN22_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNS42954.1"
                     /translation="QNVQGAIIKGVENQYDWDFFKEHLKEGQIPVFDAAKVSNEILIS
                     KKIATMLNYQLNDTVRAYFVRSAPVLKKLIIKGIYETGLEDHDKKFAIGDLRMVQELN
                     DWGIQTSIDIDDTLYSVPGYESQLIVRVQAKGGKGKFRYDWGRGNEVYGAKTINAEKD
                     TTYRVIVSDFSTNLLEKKSLSIEDTAYLKITVSGNKNAINSFETEDGTLVKNYLNEEG
                     TKYTINAGEKTITVEITNGKGSYQNYIAGFELKVNDWKQLDAIRKEVKAKVEFIPSEA
                     GEVFRVQSIKEAESDIFVWLDFLDINVAIIIVLMILIGIINMGSALLVLILVRTNFIG
                     ILKSMGATNWSIRKVFLIQAGFLIVRGMVIGNAIGLSIACLQYYFNIIPLNPEVYYLD
                     TVPIQLSLLDWLLLNVGTLIVCVAALIIPSVVITRISPSKAVRFN"
     gene            complement(1312..>2629)
                     /locus_tag="PKN22_09370"
     CDS             complement(1312..>2629)
                     /locus_tag="PKN22_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNS42955.1"
                     /translation="SFLEKGSSKGHDVATLEKIWKDWEAFASYAFNKSHSTCYAWVAY
                     QTAYLKANYPAEYMASVLSNNMNDIKQVSFFMEECRRMGINVLSPDVNESHMKFTVNK
                     AGDIRFGLGAIKGLGNGPVSAIIAEREANGPFKSIFDFAKRVSSRECNKKAYEGLILG
                     GGFDSFKNIHRAQFFFEDNNKRTFLESLIKYGSNHKETEDSAQSSLFGDDAEVDMPEP
                     TIPVCEEWSNWLKLSREKEVIGIYISGHPLDDYKLEMQTFCTGTVEMLNEPEKHLNKS
                     VVIGGYISAAEHRFSMKGSPFGTLVIDDYTHSARLALFQDNYLKNKPYITQGIFVAIR
                     GRFILNYRKEPDFEVTGIELLQNLLNKGINSIQIKVPARSLDQKIISTLNNLFLENAG
                     NTVVNFSIFDPIDEMEVNFSSKNVKVNITNEFINKLNSLDLKYKLN"
BASE COUNT          817 a          460 c          507 g          845 t
ORIGIN      
        1 acaaaatgtg caaggggcta taattaaggg agttgaaaat caatatgact gggatttttt
       61 taaagaacat ttgaaagaag ggcaaattcc tgtttttgat gcagctaaag tttccaatga
      121 aattttgatt tcaaagaaaa ttgcaaccat gctcaactat caactcaacg atactgttcg
      181 ggcttatttt gtgcgaagtg cgccggtttt gaaaaaatta atcatcaaag gtatttacga
      241 aactgggttg gaagaccatg ataagaaatt tgcaattggc gatttgcgca tggtgcaaga
      301 actcaacgat tggggaatac aaacatcgat agatattgac gataccttgt attcagtgcc
      361 aggatatgaa agtcaattaa ttgtgcgtgt tcaagcaaaa ggagggaaag gtaaatttcg
      421 ttacgattgg gggcgtggca acgaagtcta tggtgctaaa accatcaatg cagagaaaga
      481 caccacctat cgtgtaatag tttctgactt ttctaccaat ttattggaaa aaaaatcatt
      541 gtcgattgaa gataccgcct atttgaaaat cactgtttct ggaaataaaa atgcaataaa
      601 ttcttttgaa actgaagatg gaactttggt aaaaaactac ctcaatgagg agggaacaaa
      661 atacaccatc aatgcaggtg agaaaacaat aacagttgaa attaccaatg gaaaagggag
      721 ttatcaaaat tatattgctg gatttgaact taaagtaaac gattggaaac aattggatgc
      781 aattcgcaaa gaggtgaagg caaaagtaga atttatacct tcggaggcag gcgaagtttt
      841 tcgagtacaa agcattaaag aagcagaaag tgacattttt gtatggttgg attttttgga
      901 tatcaacgtg gccatcataa tagtgctgat gattttgatt ggaataatca atatggggtc
      961 ggctttgttg gtgttgattt tggtgcgcac caattttatt ggaattttaa aatcaatggg
     1021 ggcaaccaat tggagcatcc gaaaagtgtt tttaattcaa gctggatttt taattgttcg
     1081 cggaatggtt atcggtaatg caataggatt aagcatcgct tgtttgcaat attatttcaa
     1141 cattatcccg ctcaacccag aagtttatta cttggatact gttccaattc aactgtcatt
     1201 actagattgg ttgctattaa atgttggaac actcattgtt tgtgttgctg ctttaattat
     1261 tccaagtgtg gtaattaccc gcatcagtcc atcaaaagcc gtacgtttta actagttcaa
     1321 cttgtatttt aagtccaagc tgttcagttt gttaatgaat tcgttggtta tatttacttt
     1381 tacgttttta gaactaaaat tcacttccat ttcatcaatc ggatcaaaaa ttgagaagtt
     1441 aaccaccgta tttccagcat tttctaagaa taaattattt aaagtagaaa tgattttttg
     1501 gtccaacgaa cgtgcaggaa ctttaatttg aatagaattt attcctttat tgagcaagtt
     1561 ctgcaataat tcgatacctg taacttcaaa atcaggttct tttctgtagt taagaataaa
     1621 tctacctcta attgccacaa aaattccttg cgttatatag ggtttgtttt ttaaataatt
     1681 atcctggaaa agtgcaagac gtgcagagtg agtgtagtcg tcaatcacca acgttccaaa
     1741 cggagaacct ttcattgaga aacggtgttc ggctgctgag atgtatccac caataacaac
     1801 acttttatta aggtgttttt ctggttcgtt caacatttca acggtacccg tacaaaatgt
     1861 ttgcatttct aatttgtaat catccaatgg atgcccagaa atatagatgc caattacttc
     1921 tttttctcgg gatagtttta gccaattgct ccattcttcg caaactggaa tagtaggttc
     1981 tggcatatca acttcggcat catccccaaa aagtgatgat tgtgcagaat cttctgtttc
     2041 cttgtggttt gaaccgtatt tgattaaact ttccaagaag gtacgtttat tattgtcttc
     2101 aaagaaaaat tgagctcggt gtatgttttt aaacgaatca aatccacctc ctaaaatcaa
     2161 cccttcgtag gcttttttat tacattctct agaacttaca cgtttagcaa agtcaaagat
     2221 ggatttaaac ggaccattcg cctcgcgttc tgcaataatt gctgatactg gaccatttcc
     2281 caatccttta atggctccca atccaaaacg aatatcgccg gctttgttta ccgtaaattt
     2341 catgtgcgat tcgttgacat ctggactcaa gacattgatg cccattctgc ggcattcttc
     2401 cataaaaaat gaaacctgtt taatatcgtt catgttgttg gaaagcaccg atgccatgta
     2461 ttctgccgga tagttggctt tcaaataggc tgtttgatac gcaacccacg cataacaggt
     2521 agagtgcgat ttgttgaacg catacgacgc aaatgcttcc cagtccttcc aaattttctc
     2581 caatgttgca acatcatgcc ctttgcttga gcctttctcc aagaacgat
//
LOCUS       DAOQVQ010000346         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_47129_length_2620_cov_3.718129, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000346 DAOQVQ010000000
VERSION     DAOQVQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47129_length_2620_cov_3.718129"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1172)
                     /locus_tag="PKN22_09375"
     CDS             complement(312..1172)
                     /locus_tag="PKN22_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNS42956.1"
                     /translation="MGIIFVIVGIVLFVSLYDYFTSRSWQQVTSTTRNDIVFSDRNKE
                     FGAYEIRKNYDRNLILILFGVVATIGLVYGTYLFIKSLPEEEVELPPVDTTLFTIEAP
                     PLDEEVPPPPPIEEEIPVQMEKTVEFLPPVVTDEQVDTPPPIQEAMTDTKASTTTNDK
                     ETETFLPPPVQEKKQVEEKEEILTFVAEEASFPGGTAEMMKYLAKNIKYPEVAIQAGI
                     QGKVTLRFVVGKDGNIENVTVARGVPGCPECDKEAVRVVKSMPNWKPAKNDGKVVKSY
                     FNLPVTFKLQ"
     gene            complement(1179..1814)
                     /locus_tag="PKN22_09380"
     CDS             complement(1179..1814)
                     /locus_tag="PKN22_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS42957.1"
                     /translation="MAQIVDAGGGDGKGKKKAKKGTPMMDMTPMVDLAFLLLTFFVMT
                     STFSKPKVMSLVYPAKPDPKAEKTEQKINNAVTFLLSNDRIFYYQDQFYPEGNAAGKP
                     ATQLEETDFSPQGVRKLLTDLNRFVLEKKAGYDRQLKSKEIADSTYLRLMRQAKSDNA
                     ALKVLIKTDDKATCNNFIDLVDELKIADIGVIAPVDISADEFKLVKAKTDK"
     gene            complement(1818..2504)
                     /locus_tag="PKN22_09385"
     CDS             complement(1818..2504)
                     /locus_tag="PKN22_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS42958.1"
                     /translation="MAKASKPKGAPAIDMTPMVDLAFLLVTFFMLAANFRSDEVVQVD
                     TPYSISEMTIPENVVMITVDKGGRVFFGISGKPEAKENLLKRMAAKYKVPFSQEQIEK
                     FGILTDFGCSMQEMPAFLDMSSDDRKRLVAEGKTMAIPTDSTNNQLKDWINFGNIEML
                     AVGEKAYYEAKDKGFDPNINEFKPKFILKVDGKAEYVYAKKVIETFRDLNINNLNFVT
                     GLKADPRKSQ"
BASE COUNT          733 a          503 c          428 g          956 t
ORIGIN      
        1 cactgcacgt cggtcttact gggttctaaa atcccagttc aaaatatatt ttaaaagttt
       61 cttactcttt ttttgtgtga tataatctgg gatgagaagt ttataccgtc gtaattttca
      121 aggatgagcg acgtagaaaa taagaacggt gaatccaggt ttaatcacaa aaagcctctc
      181 taaaattcag ggaattgtta atgaatttga acgtatttaa tacattgata actttacctg
      241 ttaattgata aaacaaaaaa atccctaact caatttgaat tagggattta tattgtaaaa
      301 cttaaaaatt tttattgaag tttaaatgtt acaggcaagt tgaaataact ttttacaact
      361 ttaccatcat ttttagctgg cttccagtta ggcatgcttt ttacaacacg aactgcttct
      421 ttgtcacact caggacaacc tggaacacct ctagctactg ttacattttc aatgtttccg
      481 tctttaccaa ctacgaagcg caacgttact ttaccttgaa ttccagcttg aattgctact
      541 tctggatact tgatgttttt tgccaagtat ttcatcattt cagcagttcc tccagggaaa
      601 gaagcttctt cggcaacgaa tgttaagatt tcttcttttt cttccacttg ttttttctct
      661 tgaacaggtg gaggtaagaa tgtttccgtt tctttatcat ttgttgttgt tgaagctttg
      721 gtatctgtca ttgcttcttg aattggtgga ggagtatcca cttgttcatc cgttacaaca
      781 ggaggtaaaa actcaactgt tttttccatt tgaacaggaa tttcctcttc aattggtggt
      841 ggtggtggaa cttcttcatc caagggtggt gcttcgatag taaacaatgt tgtgtctact
      901 ggtggcaact ccacttcttc ttctggcaac gatttgataa acaaatacgt tccataaaca
      961 aggccaattg ttgcaactac accaaacaaa atcaaaatta aatttcggtc gtaatttttt
     1021 ctgatttcat aggctccaaa ctctttattt ctatcgctaa aaacgatatc gtttcgagtt
     1081 gtagaagtta cttgttgcca gcttcttgat gtaaaataat catacaaaga aacaaacaac
     1141 acaattccta cgattacaaa tataattccc atagtaaatt atttatctgt tttagcttta
     1201 actaacttga attcatcagc agagatgtca actggtgcaa ttaccccgat gtcagcaatt
     1261 tttaattcat ctactaagtc aataaaatta ttgcaagttg ctttatcatc ggttttaatc
     1321 aacactttta aagctgcgtt atcacttttc gcttgtctca ttaaacgaag ataagttgaa
     1381 tcagcgattt ctttgctttt taattgtcta tcataacctg cttttttctc aagtacaaaa
     1441 cggttcaagt ctgtaagtaa ttttcttact ccctgaggac taaaatctgt ttcctctaat
     1501 tgagtagctg gttttccagc agcatttccc tctgggtaga attggtcctg atagtagaat
     1561 attctatcgt tggacaacaa aaaagtaacg gcattgttaa ttttttgttc tgttttttcc
     1621 gctttcggat ccggttttgc cgggtaaacc aaactcataa cttttggttt actgaaagtg
     1681 gatgtcatta cgaagaaagt aagcaataag aatgccaagt ccaccattgg agtcatgtcc
     1741 atcattggtg tgcctttctt ggctttcttt ttacctttgc cgtctccgcc tcctgcatct
     1801 acaatttgtg ccatttctta ttgagatttt ctcggatctg cttttaaccc agtaacaaag
     1861 ttaaggttgt taatgttcaa atcacgaaat gtttctatta ctttttttgc ataaacatac
     1921 tctgcttttc catccacttt caaaatgaat tttggtttaa attcattaat gtttggatcg
     1981 aatcccttgt ctttcgcttc ataataagct ttttcaccaa ctgctaacat ttcgatattt
     2041 ccgaaattaa tccaatcttt caattggttg ttggttgaat ccgtagggat agccattgtt
     2101 ttgccttctg caactaatct ttttctatcg tcgctggaca tatctaagaa tgccggcatt
     2161 tcctgcatac tacatccaaa atcagttaga attccaaatt tctcaatttg ttcttgagaa
     2221 aaagggactt tgtattttgc agccatacgt tttagcaaat tttcttttgc ttcgggtttg
     2281 cctgaaattc caaaaaatac tctacctcct ttgtcaacag taatcatcac cacgttttca
     2341 ggaattgtca tttctgaaat actgtatggt gtatccacct gaactacttc atctgaacgg
     2401 aaattagccg caagcataaa gaatgtaacc agcaaaaacg ccaagtccac cattggtgtc
     2461 atgtcaatcg caggggcgcc ttttggtttg ctagcttttg ccattttatt ttatttttcc
     2521 gttaatgaat tattttacag attttgagaa gtcttgaaca attgtaaaac ttgcttcatc
     2581 catgctgtgt gtcatttggt caatcttagt tgagaacatg
//
LOCUS       DAOQVQ010000347         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_47198_length_2618_cov_3.563402, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000347 DAOQVQ010000000
VERSION     DAOQVQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47198_length_2618_cov_3.563402"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKN22_09390"
     CDS             complement(<1..832)
                     /locus_tag="PKN22_09390"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNS42959.1"
                     /translation="MKNVAVIGAGTMGNGIAHVFAQFGYKVSLIDISQASLDKGMATI
                     AKNLDRQVAKGALTEEEKNTTINNLTTFTSIEEGVKNVELVVEAATENVDLKLKIFAD
                     LDKFTAPNVILASNTSSISITKIASVTNRPDKVIGMHFMNPVPVMKLVEIIRGYSTSD
                     EVTEIIMETSRKLSKVPVEVNDYPGFVANRILMPMINEAIITLHEGVAGVEEIDTVMK
                     LGMAHPMGPLQLADFIGLDVCLAIMEVLYEGLGNPKYAPCPLLVNMVTAGKKGVKSGE
                     G"
     gene            complement(948..1745)
                     /locus_tag="PKN22_09395"
     CDS             complement(948..1745)
                     /locus_tag="PKN22_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42960.1"
                     /translation="MKKISYLVACCTIALAVVSCKKEGCTDPTATNYTVGATKDDGSC
                     QYANQTVPTAPTYNPVFAGTFGTLVALKVVSTTSTPIGNVDSNIGTAVAAFSENGGNS
                     FVGAGTVKVNSNALSVSSNNAYYYNVSSSNPTGIDYTASSTVNWEADGSTWPAFTAST
                     SQGFATVGTITSGNPSTSSSYTVTASSHTNADSTLFMLVGQSGNVMKIVAGAQTQYTF
                     SASEVSSCGAGTGIVEVVGLKYDLQNIASKDYYLINETARTKTVTIE"
     gene            complement(1827..>2618)
                     /locus_tag="PKN22_09400"
     CDS             complement(1827..>2618)
                     /locus_tag="PKN22_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNS42961.1"
                     /translation="DRMAAFRALNVDAIVHIFPNKDIASLAKKVKIPTRIGTSHRSFH
                     LLTCNHRLNFTRKNSNLHEAQLNFELLKPFGLKQIPTLEEINQSTAAFHPSAVELPQN
                     VQSFINQHSKYVILHPKSQGSALEWPIEKYVQLAKKLVEKGYGVAYTGTDKEGVNFRG
                     NITTSENIMDTTGTMTLEQLMRFIQQASGIVACSTGPLHIGGFLGIKSIGLFSNRKPI
                     HPGRWHAIGSDAHYIVNDENCEVCKKGKPCKCIENIEVERVATLF"
BASE COUNT          797 a          531 c          418 g          872 t
ORIGIN      
        1 aaccttcacc cgatttaact ccttttttac cagcagttac catgttcacc aacaacggac
       61 aaggagcgta ttttggatta cccaatcctt cgtacaatac ttccatgatt gccaaacaaa
      121 catccaaccc gataaaatca gccaattgca atggtcccat tggatgcgcc atacctaatt
      181 tcattactgt gtcaatttct tcaacacctg ccacaccttc gtgcaaagta atgatggctt
      241 cattaatcat tggcattaaa atacgattgg ctacaaatcc aggataatcg tttacttcca
      301 ctggcacttt acttaattta cgagaagttt ccataatgat ttcagtcacc tcatccgaag
      361 tagaatatcc tctgatgatt tcaaccaatt tcattactgg aactggattc atgaagtgca
      421 ttccaattac tttatcagga cgattggtaa ccgatgcaat ttttgtaatt gaaatagatg
      481 aagtattaga agctaaaatt acgtttggag cagtaaattt atccaaatcg gcaaaaatct
      541 ttaatttcaa atctacattt tctgtagctg cttctactac caattctaca ttttttactc
      601 cttcttcaat actggtaaaa gtagttaaat tgttgatggt tgtatttttt tcttcttctg
      661 tcaacgcccc ttttgccact tgacgatcca aatttttagc aattgtagcc attcctttat
      721 ccaaagaagc ttgagaaata tcgattaaag acactttgta tccaaattga gcaaatacgt
      781 gggcaattcc atttcccatt gtacctgcac caataactgc aacgtttttc attttcatta
      841 tttttaaact acacaaatgt ataagaaata gtcgaaacag caaattgata aaaacaaaaa
      901 agtcgctatt ctacaacagc gacttttcaa taaattttag aatacgatta ttcaattgta
      961 actgtttttg tgcgagcagt ttcattgatt aagtaataat ctttacttgc aatgttttgt
     1021 aaatcatatt tcaaaccaac tacttcaaca atacctgttc cagcgccaca agaactaact
     1081 tcagaagcac taaaagtata ttgtgtttgt gcacctgcaa caattttcat tacgttacca
     1141 ctttgaccaa ctagcatgaa caatgtagag tctgcattgg tgtgtgagct agcagtcacc
     1201 gtatagcttg aagatgtgct tgggtttcca gaagtaattg ttccaactgt agcaaatcct
     1261 tgtgaagtag atgctgtaaa agccggccaa gtgcttccgt ctgcctccca gttaactgta
     1321 gaagatgccg tgtagtcaat tcctgtagga ttgctagacg aaacattgta atagtaagca
     1381 ttgtttgaac ttacgctcaa agcatttgaa tttactttaa ctgttccagc acctacaaac
     1441 gagttccctc cgttttcaga aaaagctgct actgctgtac cgatattgct atcaacgtta
     1501 ccaattggag ttgatgttgt agaaactact tttaatgcaa ctaaggttcc aaaagtacca
     1561 gcaaaaacag gattatacgt tggagccgta ggaactgttt gattggcgta ttggcaagaa
     1621 ccatcgtctt ttgttgcacc taccgtatag tttgtagccg taggatcggt acatccttct
     1681 tttttacatg aaactactgc taaagcaatt gtgcagcaag caaccaaata agaaattttt
     1741 ttcatcgtga tttattttaa taattctaat cacaaatata aattatttta tcgccacaaa
     1801 aaaatacgaa attctaccta ttttggctaa aataatgttg caacgcgttc cacctcaatg
     1861 ttttcaatac atttacaagg tttacctttt ttgcacacct cacaattttc atcgtttaca
     1921 atataatgtg catcagaacc aatggcgtgc catctacctg gatggattgg ctttctatta
     1981 gagaacaaac caatggattt aatccctaaa aaaccgccaa tatgcaaagg tcctgtagaa
     2041 catgctacaa tacccgaagc ttgctgaata aaacgcatca actgttctaa agtcatcgta
     2101 cctgttgtat ccatgatatt ttcagaagtt gtaatgttcc ccctgaaatt cactccttct
     2161 ttatcggtgc ctgtgtatgc aacaccgtag cctttttcaa ccagtttttt tgctaattgc
     2221 acatattttt caattggcca ttccaacgca ctcccttgcg attttgggtg taaaatcaca
     2281 tatttagaat gttgattaat aaacgattgc acattttgag gcaactctac cgctgaagga
     2341 tgaaatgctg ctgtgctttg gttaatttcc tcaagtgttg gaatttgttt taacccaaac
     2401 ggtttgagta actcaaaatt caactgtgct tcatgcaaat tagaattttt tcgtgtaaag
     2461 tttaatcgat ggttacaagt cagtaaatga aaacttctat gcgatgttcc aattcttgtt
     2521 ggtattttta cttttttggc taatgaagct atgtctttat taggaaatat atgaactatg
     2581 gcatctacat ttaatgctct gaatgccgcc attctgtc
//
LOCUS       DAOQVQ010000348         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_47471_length_2609_cov_3.402897, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000348 DAOQVQ010000000
VERSION     DAOQVQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47471_length_2609_cov_3.402897"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKN22_09405"
     CDS             complement(<1..820)
                     /locus_tag="PKN22_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNS42962.1"
                     /translation="MKLIVSFLFISLLFSSVAQISSLKKDTQEVPCYEKAKTKRAQGI
                     DLWECGKLAGVIDCNEELEFDHESGLFLKKAKDNVNMMGVGKPYSGSCESCFMNGLLE
                     RRITFVDGRENGIDTSYYQDGCIQAIRSHIQGAESGTWYYYYDSTQVLAWEMNYYLGQ
                     KNGKQVYFKANGDTTRIEHYKDGVLNGVKKQYYKGSKIYKEVNYANGLLDGVFKVYNQ
                     QGVVIEESNYKANKKNGAQKYYYDDGVLLRTENWLNGVKSGEFKITDTIATVKSK"
     gene            complement(876..1481)
                     /locus_tag="PKN22_09410"
     CDS             complement(876..1481)
                     /locus_tag="PKN22_09410"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNS42963.1"
                     /translation="MKKLMFALSSVLLIACSNSNNEQPDQIAESTTPFNETAYLQKGD
                     SITSIAQGILMKNVAEAIAQKGVAGAVEFCNLKAIPLTDSISKTTLSSIHRFSDKNRN
                     PTNAITTTLDKNAWTTLASRLKDSTIKEKHFIVQEGKDVFYYKPIKIAMPTCLKCHGE
                     PNKDIAPETLQTIVKKYPQDKATNYKEGDLRGMWRVKMNEL"
     gene            complement(1493..1729)
                     /locus_tag="PKN22_09415"
     CDS             complement(1493..1729)
                     /locus_tag="PKN22_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42964.1"
                     /translation="MKQLFSNWNILRIARIALGVFIIGEGINSGQWGFIVIGAMFTIM
                     PLLNIGCCANNSCSTPINNTSSSYQEDVVFEEIK"
     gene            complement(1730..2059)
                     /locus_tag="PKN22_09420"
     CDS             complement(1730..2059)
                     /locus_tag="PKN22_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNS42965.1"
                     /translation="MNWLLLIIGGLFEVGFTFSLGKAKETDGNASLLWYAVFGFCLVA
                     SMLLLMKATQTLPLGTAYAVWTGIGAVGTVLVGIFVFNEPATIWRLFFIFTLIASIIG
                     LKAVSTH"
     gene            complement(2151..>2609)
                     /locus_tag="PKN22_09425"
     CDS             complement(2151..>2609)
                     /locus_tag="PKN22_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS42966.1"
                     /translation="GFNYTCSASNCQYNGGVAGCAELTGTATAAQIGTYNIVIKISAT
                     IMIGPFPTPIPREFTGYRLVIKDEEASTVLINPDEVYIYPNPASTVVTILNAHNFESA
                     EIYGINGQMVASQEISKTDEEINVSALQSGVYFIHLKKGNTTSIHKFTKN"
BASE COUNT          774 a          487 c          436 g          912 t
ORIGIN      
        1 ctttgctctt tacagttgcg atagtgtctg taattttaaa ttcacccgat ttaacgccgt
       61 tgagccaatt ttctgtacgc aacaaaacac catcgtcgta atagtatttt tgagcaccat
      121 ttttcttgtt ggctttgtaa tttgattctt caataactac tccttgttgg ttatacactt
      181 taaaaacgcc atccaacaaa ccattggcat agttcacttc tttgtaaatt tttgaacctt
      241 tgtaatattg ttttttaaca ccatttaaaa ccccgtcctt gtagtgttcg atgcgtgttg
      301 tgtctccatt ggctttaaaa taaacttgtt ttccgttttt ttgtcccaaa taataattca
      361 tttcccaagc caaaacttgt gttgaatcgt agtaataata ccacgttcca gattcagcac
      421 cttgaatgtg tgagcgaatt gcttggatgc agccatcttg ataataagaa gtatcgattc
      481 cattttcacg gccatccaca aaggtaattc gacgttccaa aagcccgttc ataaaacatg
      541 attcacagga acctgagtag ggttttccaa cccccatcat gttgacgttg tcttttgctt
      601 ttttcaaaaa caaaccgctt tcgtggtcaa actccaattc ttcgttgcaa tcaatcacac
      661 cggccaattt cccacattcc cataaatcga tgccttgtgc acgtttggtt tttgcttttt
      721 cgtagcaagg gacttcttgg gtgtcttttt ttagcgaaga aatctgtgca acgcttgaaa
      781 acaagaggga tataaacagg aaagaaacaa ttaatttcat agttatcaat ttattttttt
      841 tggtgggcaa taatcgtacc tttttgttgg ataacttaca attcgttcat ttttacacgc
      901 cacattcctc ttaaatctcc ttctttgtaa ttggtagctt tatcttgagg atattttttc
      961 acaattgttt gcaatgtttc tggagcaata tctttattag gttcgccatg acattttaag
     1021 catgtaggca ttgcaatttt gattggtttg taataaaaaa cgtcttttcc ttcttgaaca
     1081 atgaagtgtt tttctttaat agtgctatct tttaaacgag aagctaaggt tgtccaagcg
     1141 tttttatcca aagttgttgt aattgcattg gttggatttc tatttttatc agaaaaacga
     1201 tgaatagacg aaagtgtagt ttttgaaatt gagtctgtaa gcggaattgc ttttaagtta
     1261 caaaactcta cggcaccagc aacccctttt tgagctattg cttctgccac atttttcatt
     1321 aaaatacctt gtgcaatact tgtgatgctg tctccttttt ggaggtaggc agtttcgtta
     1381 aatggtgtag tgctttctgc aatttgatct ggttgttcgt tgtttgaatt agaacaagcg
     1441 attaataata cagaactaag ggcaaacatt aattttttca tgagaataaa ttttatttaa
     1501 tttcttcaaa tacaacatct tcttggtatg atgaggatgt gttatttatt ggtgtagaac
     1561 aactgttgtt agcacagcaa ccaatattta atagtggcat tattgtaaac attgctccaa
     1621 taacgataaa tccccactgt ccagaattga ttccttcgcc aataataaaa actcctaatg
     1681 caatacgtgc aatgcggaga atgttccaat ttgaaaatag ttgtttcatt taatgtgttg
     1741 atacagcttt taatcctatg attgatgcaa taagtgtaaa gataaaaaat aatcgccaaa
     1801 tagtagcggg ttcgttaaaa acaaaaattc ccaccaaaac ggttccaaca gctccaatgc
     1861 ccgtccacac tgcataagca gtccctaaag gcaatgtttg cgtagctttc attaataaaa
     1921 gcatgcttgc aactaaacaa aatccaaaaa ctgcatacca aagtagggaa gcatttccat
     1981 ccgtttcttt agctttgcct agtgaaaaag tgaagccaac ttcaaacaat ccaccaataa
     2041 ttaataataa ccaattcatt acaagcaaaa ttacggttta tattcgatta tatctcaaaa
     2101 aaaaacgaga acccaaagag tcctcgtttt tcaaaatcta agtttgaact ttaatttttt
     2161 gtgaatttat gaatgcttgt tgtgtttcct ttctttaaat gaataaagta aacacctgat
     2221 tgcaatgcag atacgttgat ttcttcgtca gtttttgaaa tttcttgaga agccaccatt
     2281 tgtccgttaa ttccataaat ttctgccgac tcaaagttgt gggcattcaa aattgttaca
     2341 actgttgatg caggattcgg gtaaatatac acttcatcag gattgattaa taccgtgctt
     2401 gcttcctcat ctttgattac taaacgataa cctgtaaact cacgtggaat tggagttgga
     2461 aatggcccaa tcataatggt tgcagagatt ttaattacaa tgttgtaagt tccaatttgt
     2521 gctgctgttg ctgtacctgt aagttctgca caacctgcca cacctccgtt gtattgacaa
     2581 ttggaagctg aacaagtgta attaaatcc
//
LOCUS       DAOQVQ010000349         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_47549_length_2606_cov_3.319091, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000349 DAOQVQ010000000
VERSION     DAOQVQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47549_length_2606_cov_3.319091"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKN22_09430"
     CDS             complement(<1..282)
                     /locus_tag="PKN22_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42967.1"
                     /translation="MIRFNTILANTTKVAFSSLVMLGSAASFAQTMEFTLDANNVFPT
                     LSNGAIKFGDIDGDGDLDIFMSGKNGYDYYSQIYVNNDGVFTLQTGTTIT"
     gene            553..1725
                     /locus_tag="PKN22_09435"
     CDS             553..1725
                     /locus_tag="PKN22_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNS42968.1"
                     /translation="MISFENTQVAFESKSDADLNRAYQLFKLIGNRNLVKIGKPLTNF
                     ALAIGLPIKGLIKKTIFAQFCGGETIDGCDEKIQTLGKFGIGTILDYSVEGKTSEEDF
                     EHTTNEIIATILKGKNNAFIPFAVFKVTGIARFEILEKANRTVDNLNNIELEEYKHVV
                     ARVERICKAAYDNKIPVFIDAEETWIQTTIDRITLDMMRLFNKETAIVYNTIQMYRHD
                     RLAFLKEEITLAKNEGFHYGVKLVRGAYMEKERERAAKKGYSSPIQPDKNACDKDYNL
                     ALEYIVNEIDHVALCAGSHNEESAQFLAELMDKKGIQHNDSRIYFAQLLGMSDHISYN
                     LSKLEYNVAKYVPYGPIKEVMPYLLRRADENTSVAGQTGRELSLIIKERNRRKLKN"
     gene            complement(1722..2318)
                     /locus_tag="PKN22_09440"
     CDS             complement(1722..2318)
                     /locus_tag="PKN22_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNS42969.1"
                     /translation="MIDWLEAIDRNIVLTINSWNSPLMDDIMWTFSASITWIPLYLFL
                     IYLGFKKLGWKKTLLFIGSVLLAVGCTDFICSGIIKETVQRYRPSHNLLLTNSLHFFQ
                     FEDGSYYKGGQYGFVSSHAGNFFTIAWTAGWILQAHYKRLLPTLLILATIISYSRIYL
                     GVHYLSDIIGGFLVGTTVAILVYRYFYLKFEKRITDEN"
     gene            complement(2311..2574)
                     /locus_tag="PKN22_09445"
                     /pseudo
     CDS             complement(2311..2574)
                     /locus_tag="PKN22_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
BASE COUNT          914 a          445 c          459 g          788 t
ORIGIN      
        1 tgtaattgta gtgcctgttt gcaacgtaaa aacgccgtca ttattcacat aaatttgaga
       61 ataataatcg tacccatttt ttccagacat aaaaatatct aagtcgccat cgccatcaat
      121 gtctccaaac tttattgctc cattggataa ggttggaaaa acattgtttg catctaaggt
      181 aaactccatt gtttgcgcaa atgaagcagc tgagcccaac ataaccaaag aagagaatgc
      241 cacttttgtt gtgttggcta aaattgtatt aaatctaatc atcgctaatt ttttttgtat
      301 aaccctacaa aattagggta aagcattggt aactaatact ttcgacgaat gggtaagatt
      361 gctaccgttt ttttgcaata cacaacttac taaaacattt tagtaactga ttttatggta
      421 gttacaaaaa tactattaac accctttaac atttcattca ttttagcatt gcaaaagttg
      481 gggtgatttt caactaaaaa atagggggct ttaacaattc tttgcaattg taaaatttga
      541 tacatttgca ttatgatatc gtttgaaaat acacaagtag catttgaaag taaatcggat
      601 gctgatttaa acagagctta ccaattattt aagttaattg gtaatagaaa tttggtgaaa
      661 attggaaaac cgttgaccaa ttttgcattg gcgattggtc tcccaattaa aggcttgatt
      721 aaaaaaacaa tctttgctca attttgtggt ggtgaaacca ttgatgggtg cgatgaaaaa
      781 atccaaacac tagggaaatt cggtatagga acaatattgg actattcggt agaaggaaaa
      841 acatcggaag aagattttga gcatactaca aacgaaatta ttgcaaccat tttaaaagga
      901 aaaaacaatg catttattcc atttgctgta tttaaagtta cgggaattgc acgttttgaa
      961 attttagaaa aagccaaccg aacagtagat aacctcaaca atattgaatt ggaagaatac
     1021 aaacacgttg ttgctcgtgt ggaacgcatt tgcaaagcag catacgataa taaaattcca
     1081 gtttttattg atgcagaaga aacgtggatt caaacaacaa tagacagaat tactttggat
     1141 atgatgcgtc tatttaacaa agaaactgca attgtttaca ataccattca aatgtatcgc
     1201 cacgatcgtt tggcattttt aaaagaagaa attacccttg caaaaaatga aggttttcat
     1261 tacggtgtga aattagtacg tggcgcttac atggaaaaag aacgtgagcg tgctgctaaa
     1321 aaaggatatt cttcgcccat tcaaccggat aaaaatgcgt gcgacaaaga ctataacttg
     1381 gctttggaat acattgtaaa cgaaatagac cacgttgctt tgtgtgcagg ctctcacaac
     1441 gaagaaagtg ctcagttttt agctgaacta atggataaaa aaggaattca acataacgat
     1501 tcacgcattt attttgcgca attattggga atgagtgatc atatttcata caacttatcc
     1561 aaattggaat acaatgttgc caaatatgtg ccttatggtc caatcaaaga agtaatgccg
     1621 tatttattgc gcagagcgga tgaaaacaca tcggttgctg gacaaactgg tcgtgaatta
     1681 agcttgatta tcaaagaaag aaacagacgt aaattgaaaa attaattttc gtcagtaatt
     1741 cttttttcaa attttaaata gaaatagcgg taaacaagta tggccaccgt tgttcctact
     1801 aaaaatcctc cgatgatgtc ggataaataa tgaacaccta aataaatgcg gctataagaa
     1861 ataatggtcg ccaaaattaa taacgtgggc aacaagcgtt tgtaatgtgc ttgaagtatc
     1921 catcctgctg tccacgcaat agtgaaaaaa tttccagcat gtgaagacac aaaaccgtat
     1981 tgaccgcctt tgtagtagct tccatcttca aattgaaaga aatgcaaaga attggttaac
     2041 aataaattat gcgatggccg gtaacgctgt acagtttctt tgataattcc cgagcaaata
     2101 aaatcggtgc aaccaacagc taatagcaca gaaccgatga ataataaggt ttttttccac
     2161 cctaattttt taaatcccaa ataaattaaa aaaagataga gcggaatcca agtaattgag
     2221 gcagaaaagg tccacatgat atcgtccatc aagggcgaat tccaactgtt gatggttaga
     2281 acaatgtttc tatcaattgc ttcgagccaa tcaatcattt gataagtgtt tccaaattaa
     2341 atctttcaat tctgtaatac cttttcctgc aaccgatgaa atgaacacag taggaatttt
     2401 gggcaatgtt tttttcacct cagcaattag ttcatcgtcc aacatatccg attttgtaat
     2461 ggctaaaata cgttctttgt gtaacaactc aggattgtat tgtttcaatt cgttgagcaa
     2521 tgtttcgtat tcttttttga tgtcattact atcaattgac accataaaca acaccacaat
     2581 cctccccaat tcaactacgt ctcacg
//
LOCUS       DAOQVQ010000350         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_47727_length_2600_cov_3.980354, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000350 DAOQVQ010000000
VERSION     DAOQVQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47727_length_2600_cov_3.980354"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKN22_09450"
     CDS             <1..437
                     /locus_tag="PKN22_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNS42970.1"
                     /translation="FVIGRLTFSYYGVNTKFLIKKELFRPPFGGLMRKMGGIPVDRKH
                     KNNMTKVAVEEFKKTDNLFLVFTPEGTRKYNSHWKKGFYHIALEAKVPIYVAYIDYKN
                     KTGGWDSLFEPTGDVDADIKEIKKRLSKYTGKVPENGIRAED"
     gene            complement(434..631)
                     /locus_tag="PKN22_09455"
     CDS             complement(434..631)
                     /locus_tag="PKN22_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HNS42971.1"
                     /translation="MEEDMSGFSNKKRWEDDEYYYTPEGFIVFTEKYHLKRGYCCKSN
                     CRHCPYGYNKNTGKVEGKQKN"
     gene            662..1555
                     /locus_tag="PKN22_09460"
     CDS             662..1555
                     /locus_tag="PKN22_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNS42972.1"
                     /translation="MPFDTSKSIIQTLSIEEFDKRGIQLLVKRDDLIHPEVSGNKWRK
                     LKYTIEHFKQSKCSRILTFGGAYSNHLLATASACNELNIPSIGVVRGDEHTPHSNYVL
                     SRCKELGMFLYFVSREEYAMRYMTEYHQELHNLFPNTYIVNEGGASYYGMIGCQEIIG
                     ELPPFDHLFVSQGTSTTSCGLLLGLSGQQLHVVPSIKNYDSKGEMKALLLKSTINDDL
                     IEELLQTIEVHGDYHFGGYAKQTPALFDFIDSCEKNLQLPLDKIYTAKAFYGMLEELR
                     KSDKYDNSTVVFLHTGGLYKG"
     gene            complement(1552..2496)
                     /gene="fmt"
                     /locus_tag="PKN22_09465"
     CDS             complement(1552..2496)
                     /gene="fmt"
                     /locus_tag="PKN22_09465"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNS42973.1"
                     /translation="MKDMRIVFMGTPEFAVTILNKLVENQANIVAVITSPDKPAGRGL
                     KMNESAVKQYAVSKQLPVLQPTNLKDEQFLAELKNLHADLFVVVAFRMLPEVVWSMPP
                     KGTINLHASILPHYRGAAPINWAVINGEKETGVTTFFIEKEIDTGKIIERTTTAIGDN
                     ETVGELHDRLMALGAETTLSTVQKIAKGDVQGIPQIEFIEGELKIAPKIFKDDCKVNF
                     NQSAKKVHDFIRGLSPYPAAWCTLINGGEQRTFKLFASQLTTEKTNQSKQILSSKDGI
                     LFPCNNYYVLVTELQPEGKRRMDFKAYLAGNSLEGFTV"
BASE COUNT          799 a          490 c          453 g          858 t
ORIGIN      
        1 attttgttat tggtcgcctt actttttcat attatggtgt taacaccaaa tttttaatca
       61 aaaaagaatt atttcgtcca ccatttggcg gcttaatgcg aaaaatgggt ggaattccag
      121 ttgacagaaa gcataaaaac aacatgacca aagtggctgt tgaagaattt aaaaaaacag
      181 acaatttgtt tttagttttt acaccagagg gtactcgaaa atacaattct cattggaaaa
      241 aaggatttta ccacattgca ttagaggcaa aggttccaat ttacgtagct tatattgact
      301 acaaaaacaa aaccggtggt tgggacagtt tatttgagcc aacaggtgat gtagatgccg
      361 acattaaaga gataaaaaag cgattgagta aatatacagg aaaggtgcct gaaaatggca
      421 ttcgtgcaga ggattaattt ttttgtttcc cttctacttt cccggtgttt ttattgtaac
      481 cataaggaca atgacggcaa ttgcttttac aacaataccc gcgttttaaa tggtattttt
      541 cggtaaaaac aataaatccc tcgggagtgt aataatattc atcatcctcc catcttttct
      601 tattagaaaa tcctgacata tcctcttcca tattgcaaaa ttatttaatt ttagaaaaaa
      661 aatgccattc gataccagta aatccatcat tcaaacactt tccatcgaag aatttgacaa
      721 acgtggaatt caacttttgg taaaacgtga cgatttgatt cacccagaag tgtcgggcaa
      781 taaatggcga aagctaaaat atactattga acattttaag caatcgaagt gctcacgtat
      841 cctaacattt ggaggtgcat attctaacca tcttttggct acagcaagtg cttgcaatga
      901 actcaatatc ccatccatag gcgttgttcg tggtgatgaa cacactccgc attccaacta
      961 tgtgctgtcg cgctgcaaag aattgggcat gtttctttat tttgtttcgc gagaagaata
     1021 tgcaatgcgt tacatgaccg aataccacca agaattgcac aatctttttc ccaacaccta
     1081 tattgtcaat gaaggaggag ctagctacta cggaatgata ggttgtcaag aaattattgg
     1141 tgaattaccc ccgtttgatc acctatttgt ttcgcaagga accagtacca caagttgcgg
     1201 attgttgtta ggactttcgg gccagcagtt gcacgttgtt ccttctatca aaaattacga
     1261 ttcaaaagga gaaatgaaag cattgctttt aaaatcaact attaacgatg atttaattga
     1321 agaattactt caaacaattg aggtgcatgg agattatcat tttggaggat atgctaaaca
     1381 aactcctgct ctttttgatt ttattgattc ttgcgaaaaa aacttacaac tccctctgga
     1441 taaaatatat acagccaaag cattttatgg aatgctggaa gaattaagaa aatcggataa
     1501 atacgacaat tcaacggttg tgtttttaca cactggcgga ctttacaaag gttaaaccgt
     1561 aaatccttcc aatgaatttc ctgccaaata agctttaaaa tccattctgc gcttaccttc
     1621 tggttgcaat tcggttacca acacataata attattgcaa ggaaacaaaa ttccatcttt
     1681 agaacttaaa atttgctttg actgattagt tttttctgtt gtaagttggc tagcaaatag
     1741 tttaaaagtt cgttgttctc ccccattgat tagcgtgcac caagcagcag gataaggcga
     1801 taaaccgcga ataaaatcat gtactttttt agccgattga ttgaaattta ccttgcaatc
     1861 atctttaaat atttttggtg caatttttaa ttctccttca atgaattcaa tttgaggaat
     1921 accttgtaca tctccttttg caattttttg gacagtagac agggttgttt ctgcacccaa
     1981 agccattagt ctatcgtgta actcacccac tgtttcgttg tcaccaattg cagttgttgt
     2041 gcgttcaatg attttccctg tatctatttc tttttcaata aaaaacgttg tcaccccggt
     2101 ttctttttcg ccatttatca ctgcccagtt gataggcgca gcacctctgt aatgcggcaa
     2161 tatacttgcg tgtagattaa ttgttccttt aggtggcatc gaccacacca cttctggcaa
     2221 catgcggaat gctaccacca caaacaaatc ggcatgtaag tttttgagtt ctgccaaaaa
     2281 ttgttcgtct tttaaatttg ttggttgcaa cacaggcaat tgtttgctaa ctgcatattg
     2341 cttcactgca ctttcgttca ttttcaatcc tctaccagca ggtttatctg gcgaggtaat
     2401 aactgcaaca atatttgctt gattttcaac taatttattg aggatggtta ccgcaaattc
     2461 aggggttccc ataaaaacaa ttcgcatatc tttcatggtg caaagataat gggattaatg
     2521 cttttgtagt tgaatgttta gtttttgttt taaaatttaa tcaaccctca aaacttcttt
     2581 tttccaattt accaatttta
//
LOCUS       DAOQVQ010000351         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48019_length_2590_cov_3.404734, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000351 DAOQVQ010000000
VERSION     DAOQVQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48019_length_2590_cov_3.404734"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKN22_09470"
     CDS             complement(<1..685)
                     /locus_tag="PKN22_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS42974.1"
                     /translation="MIKCLIIEDEEAGQVLLKNKLTKFFPDIEIVAIIDHFNDATAYL
                     QKHEVDLAFVDVHIKGGLGLDLLKSDLLKNSVAIFITAYDKYAIEALNAGASYYFLKP
                     LSNEDFKNGMEIVLSKYFDNKQKEKLFITNTNKTIPIDYDDILFIKSDGTYSLITTDN
                     EVHVSIKNIGFYEQTLPVSLFARTHNSYLVNIQKIAALSKSRNGSIILVNGQEIPVSQ
                     RKLSSFIEQF"
     gene            complement(678..>2590)
                     /locus_tag="PKN22_09475"
     CDS             complement(678..>2590)
                     /locus_tag="PKN22_09475"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS42975.1"
                     /translation="DKSGTIYSAINKGKIMQIKNGKASLYTNMNKHVHSLNFIDNKLY
                     AGTWGEGIFILQQDKVVDSIQFQYSKNNVCYFTFKDKNQKIWIGTMNGLFIKNNNKQL
                     KKTAMTEKAISIYQLKNGNLVIGGEQIVCFFDINGKLIKKINAEDGLKCKEVRCFYED
                     KNKTLWIGTYEGGLYYYKNGKLTSVNKMQNCQLNKDVFTFAKHQNGLIYMSSNNGLWA
                     VYESSLQDFCAKRINYLIPFYYGENSGLINNEFNGGFQNNYLINNNFIYFPSIFGLIE
                     FNTLPNPTKRNEKPFLKSVVLIDTLVTTSTVFSRTTHTIAFNFSTKNFLQENNVHYQY
                     QLIHDDENSPWSLPQKGNLISLKMLEPGYYTIKIRVIDGYNHLHPTQMSYSFEILPYF
                     YETLWFKLLLLTLFIAFIIFLFSMFFRNLKKKELIKNQISELKLNAIHSKLNPHFIFN
                     TLNSVMYLMDIDKQDEAQKLIEDFSKLLRQFLNASDDSFVPLEEELRIIDLYLSIQQK
                     RYNFDYEITYAQKIVNVLIPSLIIQPFVENALVHGIAHSNKRGMIHVVVYSFDNKIII
                     KIEDNGIGREKASEINKNRTNHISIGTKLVDEKISILEKKFNKKIHLNYIDINTNNQT
                     GTLVIIEILLHD"
BASE COUNT          842 a          377 c          384 g          987 t
ORIGIN      
        1 gaaattgttc aataaagctg cttaacttac gttgagatac aggtatttct tgtccgttta
       61 ctaatataat tgacccattt ctagatttgg ataatgcggc tattttttgg atgttcacca
      121 aataagaatt atgcgtacga gcaaataaag atacgggtaa cgtttgttcg taaaagccaa
      181 tattcttgat gctaacatga acttcattat cggtagtgat gagggaatag gtgccatctg
      241 atttaatgaa taaaatatca tcataatcaa taggaatagt tttgtttgtg ttcgttataa
      301 aaagtttttc tttttgcttg ttatcaaaat acttcgataa aacgatttcc attccatttt
      361 taaagtcctc attcgacaat ggttttaaaa agtaataaga ggcaccagcg ttgagtgctt
      421 ctatagcata tttgtcgtag gcggtaataa atatggcaac actgtttttt aataaatcgg
      481 actttagcaa gtcaaggcct aatccacctt ttatatgcac atctacaaat gcaaggtcaa
      541 cttcatgttt ttgtaaatag gcagttgcat cgttgaaatg atcaattatg gcaactattt
      601 ctatgtctgg aaaaaacttt gtgagtttat ttttcaataa tacttgacca gcttcttcgt
      661 cttcaattat taaacattta atcatgcaat aggatttcaa ttataactag tgttccagtt
      721 tggttatttg tatttatgtc tatgtagttg agatgaattt ttttgttaaa ttttttctct
      781 aagatactaa ttttttcatc tactaatttc gtgccaattg aaatatggtt ggttctgttt
      841 ttattaattt cacttgcttt ttctctacca attccgttat cttcaatttt aatgataatt
      901 ttattatcaa aagaatagac cacaacatga atcattcctc ttttattcga gtgggcaata
      961 ccgtgaacaa gagcgttttc tacaaatggt tgaatgatta atgaaggaat caatacatta
     1021 acaatttttt gagcgtatgt tatttcataa tcaaaattat agcgtttttg ctgaatggat
     1081 aaatacaaat ctataattct taattcttct tctagtggca caaaagaatc atcacttgca
     1141 tttaaaaatt gacgcaataa tttagaaaaa tcttcaatta atttttgtgc ttcatcttgc
     1201 ttatcaatat ccattaaata cattacactg ttcagcgtgt taaaaataaa atgtgggtta
     1261 agttttgaat gaatagcatt gagctttaac tccgaaattt gatttttaat taactctttc
     1321 ttttttaaat tgcgaaaaaa catagaaaaa aggaagataa tgaatgcaat aaaaagggtt
     1381 aataagagaa gcttgaacca aagtgtttca tagaaataag gtaaaatttc aaatgaataa
     1441 ctcatttgtg tagggtgcaa atggttgtat ccatctatta cacggatttt gatggtgtaa
     1501 tagccaggtt caagcatctt taatgatatt aggttgcctt tttgaggcaa actccaaggg
     1561 ctattttcat catcatgaat taattggtat tggtagtgaa cattgttttc ttgtaaaaag
     1621 ttttttgtag aaaaattgaa agctattgta tgtgtagtgc ggctaaatac agtgctagtt
     1681 gtaactaaag tatcaattaa aacaacactc ttgagaaaag gcttttcatt tctttttgtt
     1741 ggattaggaa gtgtgttgaa ctcaatcagt ccgaaaatgg aaggaaaata aatgaaattg
     1801 ttattaatta aataattgtt ttgaaaacca ccattaaatt cattgttaat taatccacta
     1861 ttttcaccat aataaaatgg aattaaataa ttaatacgtt tagcacaaaa gtcttgcaaa
     1921 ctactttcat aaactgccca taaaccattg ttggatgaca tgtatatcaa accattttgg
     1981 tgtttagcaa atgtgaaaac atctttgttg agttggcaat tttgcatttt gttaaccgaa
     2041 gttaatttcc catttttata ataatacaag ccaccctcat acgtacctat ccaaagtgtt
     2101 ttatttttgt cttcataaaa acaacgtact tctttacatt ttaaaccatc ttcagcattt
     2161 attttcttta ttagtttccc atttatatca aaaaaacaaa ctatttgctc tccaccaatt
     2221 acaaggttcc cgttttttag ctgataaata gaaatagctt tttcagtcat tgcagttttc
     2281 ttgagttgtt tgttattatt ttttataaac aacccattca ttgttcctat ccatattttt
     2341 tgatttttat ctttaaaagt aaaataacaa acattatttt ttgaatactg aaactgtatt
     2401 gagtcaacaa ctttatcctg ttgtaaaata aatatgcctt cgccccatgt tcctgcatat
     2461 aatttgttgt caataaaatt taatgaatgg acatgcttat tcatatttgt gtataaactt
     2521 gctttaccat tcttgatttg cattatttta cctttgttga tggcagagta gatagtaccg
     2581 cttttatctt
//
LOCUS       DAOQVQ010000352         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48169_length_2584_cov_3.892843, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000352 DAOQVQ010000000
VERSION     DAOQVQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48169_length_2584_cov_3.892843"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PKN22_09480"
     CDS             complement(<1..1530)
                     /locus_tag="PKN22_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNS42976.1"
                     /translation="MGFRKETPKKQSSFNKLTISLASPEQILEQSSGEVLKPETINYR
                     TYKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     SLVVPVAHIWYFRSLPNKIGYLLGLPTKKLDQIIYYERYVVIQSGIAVNVDGKELKRL
                     DFLTEEEYLNILESLPVENQYLEDTDPNKFIAKMGAEAIYDLLRGLDLESTSYDLRHQ
                     ANTETSVQRKHEALKRLQVVEAMRESQEHIDNRPEWMVVKVVPVIPPELRPLVPLDGG
                     RFATSDLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSSAV
                     KTESNRPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPTLKLHECGLPKDMAA
                     ELYKPFIIRKMIERGIVKTVKSAKKIVDRKEPVVWDILENVLKGHPVLLNRAPTLHRL
                     GIQAFQPKLIEGKAIRLHPLVTTAFNADFDGDQMAVHLPLGNAAILEAQLLMLASHNI
                     LN"
     gene            complement(1558..>2584)
                     /gene="rpoB"
                     /locus_tag="PKN22_09485"
     CDS             complement(1558..>2584)
                     /gene="rpoB"
                     /locus_tag="PKN22_09485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686916.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNS42977.1"
                     /translation="AVLKKQLVDKLLKIIGEEKSAGVFNNFREEIIKKGSKFTEKNLF
                     AIDYTIVNPTNWTSDTRLNTLIGRVLHNFTIKSNDVLGAYKRKKFHISVGDELPAGII
                     KMAKVYVAKKRKLRVGDKMAGRHGNKGIVANIVRQEDMPFLEDGTPVDIVLNPLGVPS
                     RMNLGQIYETVLGWAGQKLGVKFATPIFDGAQPEHIDEWCDKAGIPRSGKTYLYDGGT
                     GERFHQPATVGVIYMLKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEV
                     WALEAFGAANILQEILTVKSDDVMGRAKAYEAIVKGDNIPEPGIPESFNVLLHELRGL
                     CLNVSLD"
BASE COUNT          759 a          562 c          420 g          843 t
ORIGIN      
        1 gttcaaaata ttgtgcgaag ccaacattaa taactgagct tccaaaattg cggcgttacc
       61 taatggtaag tgaaccgcca tctggtcacc gtcaaagtcg gcgttgaacg ctgttgttac
      121 caatgggtga agacgaattg cctttccttc aatcaatttt ggctggaatg cttggatacc
      181 caaacggtgc aaagtaggag cacggttcaa taatactggg tgtcctttca atacattctc
      241 caaaatatcc caaacaacag gctcttttct atcaacaatt ttcttagcgg atttaactgt
      301 ttttacaatt ccacgctcaa tcatcttacg aatgatgaac ggtttgtata actcagctgc
      361 catgtctttt ggcaaaccac attcgtgtaa ttttaatgtt ggtccaacaa caattaccga
      421 acgtgctgaa taatccacac gtttacccaa caagttttga cggaaacgac cttgttttcc
      481 tttcaacgag tcagacaacg attttaatgg acggttggat tctgttttaa ctgcagatga
      541 ttttcttgag ttatcaaaca atgaatctac cgcttcttgc aacatacgtt tttcgttgcg
      601 caaaatcact tccggagctt tgatttcaat caaacgcttc aaacggttgt ttctgataat
      661 tacacgacga tacaagtcat tcaagtcaga agtagcaaaa cgtccaccat ctaaaggaac
      721 taacggacgc aattctggtg gaataactgg aacaacttta acaaccatcc attctgggcg
      781 gttatcaata tgctcttgcg attcacgcat tgcctcaaca acttgcaaac gttttagtgc
      841 ttcgtgtttt ctttgaacag aagtttctgt atttgcttgg tgacgtaaat cgtatgaagt
      901 tgattccaaa tccaaaccac gtaacaaatc ataaatagct tcagcaccca ttttcgcgat
      961 aaacttatta gggtctgtat cttctaaata ttgattttct actggtaatg actctaaaat
     1021 atttaagtat tcctcttctg ttaagaaatc taaacgtttt aattctttac catcaacatt
     1081 aacagcaata cctgattgga ttactacata acgctcgtaa taaataattt gatccaattt
     1141 tttcgttggc aatcctaaaa gataaccaat tttatttgga agtgaacgga aataccagat
     1201 gtgtgcaaca ggaactacca aagaaatgtg tcccattctt tcacggcgca cttttttctc
     1261 tgttacttca acaccacaac ggtcacaaac aatccctttg tagcgaatac gtttgtattt
     1321 accacagtga cattcgtaat ctttaactgg accgaaaata cgctcacaga aaagaccatc
     1381 acgttcaggt ttataagtac gatagttaat cgtttctggc tttaaaacct ccccgcttga
     1441 ttgctccaag atttgctctg gagaagctaa ggaaatagtc aacttattaa agctgctttg
     1501 tttttttggt gtttcttttc taaaacccat actattgctt gtttctttaa ttcaaaatta
     1561 atccaatgac acatttagac aaagtcctct caactcatgt aacaatacgt taaatgattc
     1621 aggaattcca ggttctggaa tgttgtctcc tttaacaatt gcttcgtaag cttttgcacg
     1681 acccatcacg tcatctgatt taaccgtcaa aatctcttgt agaatgttgg ctgcaccaaa
     1741 tgcttccaat gcccaaacct ccatctcacc aaaacgctga ccaccaaact gagctttacc
     1801 acccaatggt tgttgtgtaa tcaacgagta tggtccgata gaacgagcgt gcattttgtc
     1861 atccaccatg tgtgataatt tcaacatgta aattacccca acagttgcag gttggtggaa
     1921 acgttctccc gttcctccat cgtataaata cgttttacct gatcttggaa ttccagcttt
     1981 atcacaccac tcatcgatat gttctggttg agctccatca aaaattggtg tagcaaactt
     2041 cactcctagt ttttgtcctg cccaacctaa tacagtttcg taaatctgtc caaggttcat
     2101 acgtgaaggt accccaagtg ggttcaacac gatatctact ggcgttccat cttctaagaa
     2161 tggcatatct tcttgacgaa cgatgttggc aacgatacct ttgttaccgt gacgtccagc
     2221 cattttatca ccgactctta atttacgttt tttagcaaca taaacttttg ccattttgat
     2281 aattcctgct ggcaattcat cacctacaga gatatggaat tttttacgtt tatatgctcc
     2341 aagcacatcg tttgacttaa ttgtgaaatt atgtaaaacg cgtccaatta acgtattcaa
     2401 acgtgtatca cttgtccaat ttgtagggtt aacaattgta taatcaattg caaataaatt
     2461 cttttctgtg aatttagaac cttttttaat gatttcttca cggaagttat tgaaaacacc
     2521 agcagatttt tcttcaccga taattttcaa taatttatct accaattgtt ttttcaatac
     2581 tgca
//
LOCUS       DAOQVQ010000353         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48200_length_2583_cov_4.109968, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000353 DAOQVQ010000000
VERSION     DAOQVQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48200_length_2583_cov_4.109968"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PKN22_09490"
     CDS             complement(<1..786)
                     /locus_tag="PKN22_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNS42978.1"
                     /translation="MSNETIQQSVARIFSPTKLGPITLPTPVIKAATSEGRSPEGKVT
                     QALIDFHLGFIKGGVGMTTLAYCAISKAGFAAPGEILMVRENIDGLKKFTQAMHDAGG
                     LASAQLGHAGPVATKQITGFQPVAPSNFRNPTSFQKCREITLKEMEEIKEQFADAAEV
                     AADSGFDAIELHFGHLYLVSSFMSPWINKRKDQYGGSIENRTRFAKEILLKVKERVKD
                     RLAIIIKLSMDDGIKGSIWLDESTQTAKILDETLQSLNSDYEAK"
     gene            complement(874..1698)
                     /locus_tag="PKN22_09495"
     CDS             complement(874..1698)
                     /locus_tag="PKN22_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNS42979.1"
                     /translation="MSEKNILKTFTSELPYNQWIETGFFTIIFIKEGTIEMTLNSKKN
                     TIQKGQILINTPFHNTFLNNVSSNCSVIGIKFSIDYLKNISILAEFYNTVPYFQYHYL
                     PVWKLNDNEQYLVQSLLEKLKNKEENKDNHTFGNQLFNVVFSEFIIELSEIGKNQDKN
                     VFHNYNRLEYITLQFYILAKKYYKEQPKLSFYADKLAVSTKYISETIKQVTGVTAKDT
                     LVNLRMAYARTVLATSNMDVAQISDALCFDSSASFSNFFKKEEGITPKEYRKLHHT"
     gene            complement(2041..>2583)
                     /locus_tag="PKN22_09500"
     CDS             complement(2041..>2583)
                     /locus_tag="PKN22_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42980.1"
                     /translation="DADGLLSVWDIDTLTLLLQLPFDSDKIRKIVVSNDEETLYLCCK
                     DGYVRSVETTYFNQKDEFYAHNDGVGTILEWDKNTLITGGKDAIIRLWDKTTKKQIKA
                     LPAHNYMIYNLVKLNEHTLVSSSRDKSIKIWNIPQWTVAQKIEYKHGGHRHSVNNITP
                     INENTFASASDDSNIILWGI"
BASE COUNT          744 a          455 c          443 g          941 t
ORIGIN      
        1 tttagcttcg taatcggaat taagactttg taaagtttcg tcaagaattt tagctgtttg
       61 agttgattca tccaaccaaa tggatccttt aattccgtca tccatagaaa gtttgatgat
      121 tattgccaat cggtctttta ctctttcttt tactttcaac aatatttctt tggcaaaacg
      181 agttctgttt tcaattgaac caccatattg gtctttacgt ttattgatcc atggactcat
      241 aaaagaagaa accaaataca aatgtccgaa atgcaattca atggcatcaa aaccagaatc
      301 tgcagcaact tcggcagcat cggcaaattg ttctttaatt tcctccattt cctttaaagt
      361 aatttcacgg catttttgga aacttgttgg gtttctaaag ttggaagggg cgacaggttg
      421 aaaaccagtg atttgcttag tagctactgg tccggcatgt ccaagttgcg cagaagctag
      481 tccaccagca tcgtgcatag cttgcgtaaa ttttttcaag ccatcaatgt tttctcgcac
      541 cattaaaatt tctcctgggg cagcaaaacc tgctttcgag atagcacaat aggccaaggt
      601 tgtcatacca actcctcctt ttataaatcc taaatgaaag tcgattaaag cctgggttac
      661 ttttccttca gggcttctgc cttcacttgt agcagctttt attaccggcg tgggtaatgt
      721 gatgggtcct aattttgttg gactaaaaat tcgagctaca ctttgttgaa tggtttcatt
      781 gctcataact aaataattaa atgttcattc taacaaagtt acggaggttt tacttaacaa
      841 taaattgaga aattaccgat aaatttgtga aaattacgta tgatgtagtt ttctatattc
      901 ttttggagta ataccttctt cctttttgaa aaaatttgaa aacgaagcag aagaatcaaa
      961 acacaacgca tctgaaattt gagcaacgtc catattactt gttgccaaca ccgttctagc
     1021 atacgccatt cggaggttga ccaacgtatc tttagcagtg acgccggtaa cttgttttat
     1081 tgtttctgaa atatatttcg ttgaaacagc taatttgtcg gcataaaaac tcagtttagg
     1141 ttgttcttta taatattttt ttgctagaat ataaaattgt aaggtaatgt attctagtct
     1201 gttataatta tgaaaaacgt ttttatcttg atttttacct atttctgata gttcaattat
     1261 aaattcactg aaaaccacat taaaaagttg atttccaaat gtatgattgt ccttgttttc
     1321 ttctttattt ttcagttttt ctaataacga ttgcaccaaa tattgttcat tgtcatttaa
     1381 tttccaaacg ggtaaataat ggtattggaa atagggcaca gtgttataaa attcggcaag
     1441 aatactgatg ttttttaaat aatctattga gaatttaatt ccaattacac tacaattact
     1501 gcttacattg tttagaaatg tattatgaaa tggagtattg attaaaattt gacctttttg
     1561 tattgtattt tttttagaat taagcgtcat ttctatcgtt ccttctttta taaaaataat
     1621 ggtgaaaaaa ccagtttcaa tccattgatt ataaggtaat tcggatgtga aagttttaag
     1681 aatatttttt tcagacactt tttttcgatt tgattctttt tgtaaagtta tttatttttt
     1741 atgaattgtg aattgtttct cttagcgtct attgcggtta aaaaaaaact taaccgcgga
     1801 gttacgccaa gaacacgcag agttcgcaga gaaaaacatt gcggccattg cgccttcttt
     1861 gcgctcattg cggttacaat ttaattctcc tttgcttcca ttgcgttaac ctttgcgctc
     1921 attgcggtta aaatttattt cctctttgcg ttcattgcgc cttcttagcg tcctttgcgg
     1981 tttttcttta atttctctct gagctcattg cggttaaaaa atacaaacct tcaactcaac
     2041 ctatattccc cacaaaataa tgttagaatc gtctgaagcg gaggcaaagg tgttttcgtt
     2101 gattggtgtg atgttattga ccgaatggcg gtgaccaccg tgtttgtatt ctattttttg
     2161 agcaacagtc cattggggaa tgttccaaat tttaatgctt ttatcgcgac tactagaaac
     2221 aagtgtgtgc tcgtttaact ttaccaaatt gtaaatcata tagttgtgtg ccggtaatgc
     2281 tttgatttgt tttttagtgg ttttatccca caggcgaata atcgcatctt ttccacccgt
     2341 aatcagcgtg tttttatccc attccaaaat agttccaaca ccatcgttgt gtgcataaaa
     2401 ttcatctttt tggttgaaat aagttgtttc taccgagcga acatagccat ctttgcaaca
     2461 taaataaagc gtttcttcat cgttggaaac aacaattttt cgaattttat cgctgtcaaa
     2521 tggaagttgt aaaaggaggg tgagcgtgtc tatatcccaa acagaaagca agccatctgc
     2581 atc
//
LOCUS       DAOQVQ010000354         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48230_length_2582_cov_3.631975, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000354 DAOQVQ010000000
VERSION     DAOQVQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48230_length_2582_cov_3.631975"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..2127
                     /locus_tag="PKN22_09505"
     CDS             166..2127
                     /locus_tag="PKN22_09505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS42981.1"
                     /translation="MKKHFLLALGFTAAFGTVAKAQQQDPVLMEIDGKKITKSEFLQI
                     YLKNNNNPKYDQKTLDEYIDLFKKFQLKVAEAEALGYDTIPKLKNELAGYQKQLAQPY
                     LTDKSMNEALVQQAYERMKNEVRASHILIKLPANPSPEDTLKAYNKAMEVRSKLLKGG
                     DFNKIASEYSDDPSAKTNFGDLGFFTAFQMVYPFEEAAYNQKIGEISMPIRTRFGYHI
                     LKVTDKRPARGTMKAAHLMVAANEENDSPETIAKAESKINELYEKLKKGDNFEELVKA
                     YSDDPSSASNGGLLPVFGTGATTRMIPEFEEAAFGLEKDGDFSKPVRSKFGFHIIKRI
                     QLTPLKSFDELKKEMETRVSRDERANLTQNSYIEKLKKEYNYSEPNHEKVMAWFNKNM
                     DSSIYKGEFNAGKLKTNAVMFSLAGKNYTEKDFVAYMNENPRNSRGTALSEVAKKQFK
                     EWTNSTVIDYEKSRLDVKYPEYKALMKEYHDGILLYEVMSDKVWNKAMTDTVGLKAFY
                     EANKANYVWGKRYDCTVYETMSEDVAKKVYKLAKKKLAADSIASIINKNTELNLKVRS
                     GKFELSKTPYLEKQPLKKGVNKPYVFDGKNYVVKLNEVIEAKNKELAEAKGIITSDYQ
                     TYLEKEWLNELEKKHKIVVYKDVLYSLGK"
     gene            2261..>2582
                     /locus_tag="PKN22_09510"
     CDS             2261..>2582
                     /locus_tag="PKN22_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNS42982.1"
                     /translation="MKLIIGLSFIVASLGVTFGQQKRTLLNGVVAQIGDNVILQSDIE
                     AQVLQAQQAGITIDESFRCNVLEGIMYQNLLINQAKLDSIEITDQQVDAEMENRIRSI
                     EAQIG"
BASE COUNT         1008 a          367 c          479 g          728 t
ORIGIN      
        1 tttaaggtgt gaagcagaat aaagtgcacc ttctttcaac gagttgagtg aaatgaagaa
       61 tttttgtgca ttcaaacctt aaaaaaactt aaataattct taaatttaac gccttattca
      121 attgagaaaa ttatttttga tgtaattgta aattaaaaac aacctatgaa aaaacatttt
      181 ttacttgctt tgggatttac agcagcgttt ggaactgttg caaaagcaca acaacaagat
      241 ccagtattaa tggaaattga tggaaagaaa atcacaaagt cggaatttct tcagatttac
      301 ttaaaaaaca acaacaatcc aaaatacgac caaaaaacat tagacgagta cattgacttg
      361 tttaaaaaat ttcaattaaa agttgcagaa gcagaagcgt tgggatacga tactattccg
      421 aagttaaaaa atgagttagc tggatatcaa aaacaattgg ctcaacctta tttgactgac
      481 aaaagcatga atgaagcgtt ggtgcaacaa gcgtatgagc gtatgaaaaa tgaagtgaga
      541 gcttctcata ttttgattaa attgcctgca aatccttctc cagaagatac tttaaaagct
      601 tataataaag caatggaagt tcgcagtaaa ttgttaaaag gcggtgattt caataaaatt
      661 gcaagtgaat attctgatga tccatctgca aaaaccaact ttggtgattt aggatttttc
      721 acagctttcc aaatggtata tccttttgaa gaagcggcat acaatcaaaa aattggtgaa
      781 atttcaatgc cgattcgcac acgttttggt taccacatac taaaagttac cgacaaacgt
      841 ccggctcgtg gtacaatgaa agctgcacat ttgatggttg ctgcaaatga agaaaatgat
      901 agtccagaaa ccattgcaaa agcagaaagc aaaatcaatg agttgtatga gaaattgaaa
      961 aaaggagaca attttgaaga gttggtaaaa gcctattcag acgatccatc ttcggctagt
     1021 aatggtggtt tattgcctgt ttttggaacg ggtgcaacta cacgtatgat tccagaattt
     1081 gaagaagctg cttttggatt ggaaaaagac ggcgatttta gtaaacctgt tcgttcaaaa
     1141 tttggtttcc acattatcaa acgcattcaa ttgacaccat taaaatcatt tgatgagttg
     1201 aaaaaagaaa tggaaactcg tgttagccgt gatgaacgtg ctaatttaac gcaaaacagc
     1261 tacatcgaaa aattgaaaaa agaatacaat tacagcgagc ctaatcatga aaaagtgatg
     1321 gcttggttca ataaaaacat ggattcttct atctacaaag gtgaatttaa tgctggaaaa
     1381 ttgaaaacaa atgcggtgat gttctcatta gcaggtaaaa attacaccga aaaagatttt
     1441 gttgcttaca tgaatgaaaa tccaagaaat tctcgtggta cagcattatc agaagtggca
     1501 aaaaaacaat tcaaagagtg gacaaattct acagttattg attatgaaaa atcacgattg
     1561 gatgtgaaat accctgaata caaagcgttg atgaaagaat atcacgacgg aattttgttg
     1621 tatgaagtaa tgagcgacaa agtttggaac aaagcaatga ccgatacagt tggattaaaa
     1681 gcgttctacg aagcaaacaa agcaaattat gtttggggaa aacgttacga ttgcacagtt
     1741 tatgaaacaa tgtctgaaga tgttgctaaa aaggtgtata aattagctaa aaagaaattg
     1801 gcagctgata gcattgctag catcatcaac aaaaacacag agttaaactt aaaagttaga
     1861 tcaggaaaat ttgaactttc aaaaacacct tatttagaaa aacaaccgtt gaaaaaagga
     1921 gttaacaaac cgtatgtttt tgatggtaaa aactatgttg ttaaattgaa tgaagtaatt
     1981 gaagctaaaa acaaagaatt agctgaagca aaaggtatca taacttctga ctaccaaaca
     2041 tatttggaaa aagaatggtt gaatgaattg gagaaaaaac ataaaattgt agtttacaaa
     2101 gatgttttat attctttagg aaaataaacc taaaaaaatc aaaaggctgg ccaactagtc
     2161 agcctttttt tatatagttg catttaaacg ttgttctata ctatcgaaaa agaaataaat
     2221 ttagatacaa aacgttgaca caaaatagta aataagatac atgaaattaa taattggttt
     2281 aagttttatt gttgcatctt tgggtgtaac ttttggacaa caaaaaagaa cattgttgaa
     2341 tggtgttgtg gcacaaattg gcgacaatgt tattttgcaa tccgacatag aagcccaagt
     2401 tttacaagca caacaagcag gtataaccat tgacgaatca tttagatgca acgttttgga
     2461 aggaatcatg tatcaaaact tgttgattaa ccaagcaaaa ttagatagta ttgaaataac
     2521 tgaccaacaa gtagatgctg aaatggaaaa ccgtattcgt tctattgaag ctcaaattgg
     2581 ta
//
LOCUS       DAOQVQ010000355         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48315_length_2579_cov_4.614501, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000355 DAOQVQ010000000
VERSION     DAOQVQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48315_length_2579_cov_4.614501"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PKN22_09515"
     CDS             complement(<1..1168)
                     /locus_tag="PKN22_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42983.1"
                     /translation="MNTYFTLTLNKDSIVRDFTLDHVYLFNQQLAKLKASFGFAGFFF
                     IYKDVKVFLDPFIRENPLELEPVWGLFNSTFLICYAYRSSDSLPHAYRATQRFYQSLN
                     NLDPIYYLPFNWQTKELVFSSAVKSPSTFSDNNLYQAFAVGKSNIFSFVTENADVVYS
                     NPTYAFLDGVRANVPFVVGGNDVRISLTTPNNYVSPFTILIIYADQQVVQVVIETDTA
                     VFGSTQIVLVDTYNNQNYLSTPDDQVPLTYFLPHPYFSNKSRDMVISFAQPGATIFKC
                     FLAETPIIGNSVIFNPELGTNGNYINDLVPISELVKGQISTAGGIINFPSGSYVRRVA
                     ERRITDTTDYYNTLIALGPTAPTRWVSTVYTTNLAGALPIPSTTEQGMVTDFNYK"
     gene            complement(1168..1575)
                     /locus_tag="PKN22_09520"
     CDS             complement(1168..1575)
                     /locus_tag="PKN22_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42984.1"
                     /translation="MFNSASGVSLTTAACNDQLLTFDLNASNNFQAQVQLVRPYKAKC
                     IKILGSQIAKISIDGVSLSNYEFNPSAEPFPFTKSNQIMTATHDPIFYFIENANTYIN
                     LIKVALIEPDQLVGTSINITIYFFDFIPSLIAE"
     gene            complement(1575..>2579)
                     /locus_tag="PKN22_09525"
     CDS             complement(1575..>2579)
                     /locus_tag="PKN22_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42985.1"
                     /translation="ENMQSWYFNMQNAEKYQGVDFGEGSIFTMLERIMDNMKKQGKET
                     MRIEEFDYPTIARIRAYLSNSSKDDEKQWLAEVHAQKLFDEEPIRPKEIPNLEHYKNS
                     YSKFSTGAAVAGSTVAATFTTLLAKMIASNTGLYPGNEVFQYKGMGRHKPHTRKHLHE
                     ILHLLKNEKKKRIACGVCNRKMDKMYRPENQKSSEDDLCQSCFDTKELSANGINPHFH
                     KFSEYDNKEEQHEAPRLFKDFTQSKFLESMGQNHLSVSVWNAGWFDRMLEALLYSESH
                     STSSIFKANLILLISKYLKTFKHLKQSNRALTTDVFQSKEHLARVIQNDPSILANYHP
                     "
BASE COUNT          853 a          408 c          436 g          882 t
ORIGIN      
        1 ttttataatt aaaatccgtc accattcctt gttctgtagt agaaggtatt gggagagctc
       61 ctgccaagtt agttgtatag actgtagaaa cccatcttgt tggcgctgta ggacccaagg
      121 cgatcaatgt gttgtaatag tcggtagtgt cagttatacg ccgttcggca actcgtctaa
      181 cgtagctgcc actaggaaaa ttaattattc ctcccgcggt agatatttgt cctttaacta
      241 attcagatat tggaaccaga tcgttaatat aattgccgtt tgtgcccaac tcgggattaa
      301 agataaccga attgccgata ataggtgttt cggctaaaaa acatttaaat atagtagcac
      361 caggttgagc aaaagaaata accatatcac gagatttgtt agaaaaataa gggtgtggta
      421 aaaaataagt aagaggtact tgatcatcag gtgtcgataa ataattttga ttattataag
      481 tatcgaccaa aacaatttga gtagaaccaa ataccgctgt atcggtttct ataacaactt
      541 gaacgacctg ttgatcggca taaattatta aaattgtaaa aggtgaaaca taattattag
      601 gtgttgtcaa cgaaatacga acatcgtttc cacctacaac gaagggtaca ttggctctaa
      661 caccatcgag aaatgcataa gtagggtttg aataaacaac atcggcattt tcagtaacaa
      721 aactaaaaat atttgatttg ccaactgcaa atgcctgata taagttgtta tcagaaaaag
      781 ttgaaggaga ttttacagct gaagaaaaaa ctaattcttt tgtctgccaa ttgaatggta
      841 aatagtatat tggatctaga ttgtttaatg attgataaaa tctttgagta gctctgtaag
      901 catgtggcaa agaatctgaa ctacgataag cgtaacaaat taaaaaagta gaattaaaca
      961 atccccagac tggttccaat tcgagaggat tttctcgaat aaatggatcc aaaaaaactt
     1021 taacatcttt ataaataaaa aagaatcctg caaaaccaaa tgaagctttt aatttggcga
     1081 gttgttgatt aaaaagataa acatgatcaa gagtaaaatc tcgtacaata gaatctttat
     1141 ttaaagttaa agtaaaataa gtgttcatta ttcggcaatt aacgaaggaa taaaatcaaa
     1201 aaaataaata gtaatattga tgctggttcc aactaactga tctggttcaa ttaacgctac
     1261 tttaattaaa ttaatatacg tgttggcatt ttctataaaa taaaatattg gatcatgtgt
     1321 cgcagtcata atttgatttg attttgtaaa tggaaacggt tcggctgatg ggttaaattc
     1381 ataatttgaa agactgacgc catcaatact gatttttgcg atttgtgatc ccaaaatttt
     1441 tatgcatttg gctttatagg gtcgaactaa ttgaacttga gcttgaaaat tgttggaagc
     1501 attcaaatca aatgttaaca attgatcgtt acaagctgcc gttgttaaac ttactccact
     1561 tgccgaatta aacattatgg atgataatta gccaatatac ttggatcgtt ttgaataact
     1621 cttgctaaat gttcttttga ttgaaatacg tctgttgtta atgctcgatt tgattgtttt
     1681 aaatgtttaa aagtttttaa atattttgat attaatagta ttaaattggc tttaaaaatt
     1741 gatgatgtcg aatgactttc actatataac aatgcctcca acattctatc gaaccaacca
     1801 gcattccaaa cacttactga taaatgattc tgacccattg attcaagaaa tttagattgt
     1861 gtaaaatctt taaacaatct tggagcttca tgttgttctt ctttattatc gtattcacta
     1921 aatttatgaa agtgcggatt gatgccgtta gctgataatt ctttagtatc gaaacaactt
     1981 tgacacaaat catcttctga tgatttttga ttttcaggac gatacatctt gtccattttc
     2041 ctattgcaaa ctccacatgc aattcttttt ttcttttcat tctttaataa atgtaatatt
     2101 tcatgtaaat gttttcgagt atgtggttta tgtcgtccca ttcctttata ttggaagact
     2161 tcatttcctg gataaagtcc tgtgtttgac gctatcattt ttgccaacaa tgtagtgaac
     2221 gtggcagcga ctgttgaacc tgcgacagcg gctccagtac taaattttga atacgaattt
     2281 ttataatgtt ccaaatttgg aatttctttt ggacgaattg gttcttcatc aaataatttt
     2341 tgagcatgaa cttcagctaa ccactgcttt tcgtcatctt tagaagaatt ggaaagatag
     2401 gcgcgtattc ttgctattgt tggataatca aattcttcaa tacgcattgt ttctttgcct
     2461 tgttttttca tattgtccat tatacgctcc aacattgtaa aaatagaacc ttctccaaaa
     2521 tcgactcctt gatacttttc agcattttgc atattaaaat accacgattg catattttc
//
LOCUS       DAOQVQ010000356         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48445_length_2575_cov_3.658730, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000356 DAOQVQ010000000
VERSION     DAOQVQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48445_length_2575_cov_3.658730"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKN22_09530"
     CDS             <1..594
                     /locus_tag="PKN22_09530"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNS42986.1"
                     /translation="NVVGDDKWANDEALVNRNLNFRSDIFELGAGVEFHYMPFRLGHD
                     RYKGTAYLLAELAVFRMNPKTHYNGEWVYLRPLGTEGQGSNLNAKKPYSLFQFAIPLG
                     LGVKFSLGKRMSIGLEYGIRLTFTDYLDDVKADSYVDPVALAEANGPMSAALSNRSLD
                     GNRYGKRGTAATKDWYSFFGLTLTFRLGDPQKCAFME"
     gene            complement(633..1226)
                     /locus_tag="PKN22_09535"
     CDS             complement(633..1226)
                     /locus_tag="PKN22_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42987.1"
                     /translation="MQKQQVYIQLIGNLAIPLLGYFWWNWSLYFILLFYTLDLLAALI
                     VVFLKARKIKQVTQNTQIPTSTFASVAIMFFALIVALFHIGSALLHPSIDFQKEIIAF
                     LTYTEMGIQQWIVLVPMVGLMAYSSYKMEFILPQLFLKQDEKTVWKNYLKEQFLMVAF
                     SAILMLISVAYHFSDLVVLLIILAVTTIYNFLQKTGK"
     gene            complement(1230..2240)
                     /locus_tag="PKN22_09540"
     CDS             complement(1230..2240)
                     /locus_tag="PKN22_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS42988.1"
                     /translation="MIKKFFFLSLFIVLISACGGKTTKNIAVQPTTSNIDSLLALYPD
                     SIPLLLFRGNEALKDYRFFDALADGAKAFRLDSLSPDTRMLYAQALNNKENRTVEEVL
                     NAQRHFQFVLFKQPENTDALVALAATYRYMQDVDNAFKYVNKALKIDKKKREAYALKG
                     SLYVDLENYSLAKSSYETAIQQDPNFYEAYFHLAALYHRENNPICLEYYQTAYELNKT
                     NPEFLYSYAYALESFEKYDDAKVYYRKMEQEKEKYYRARGYFHLGYLKQNLENQVDSA
                     IYFYSEAINTMKNYVEAYHNRGMCYEKKGLIQEAKQEYMSALKYDDKFQLSIDAYNKL
                     DR"
     gene            complement(2350..>2575)
                     /locus_tag="PKN22_09545"
     CDS             complement(2350..>2575)
                     /locus_tag="PKN22_09545"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNS42989.1"
                     /translation="ILTIIGIAVSSEKKRGGIGVNIAIGLIIVLIFIFAMKIMAVAAE
                     NVGFPTMIAAWMPNVIFSVVAAVLYRFAQK"
BASE COUNT          814 a          460 c          459 g          842 t
ORIGIN      
        1 aacgttgttg gtgacgataa atgggcaaat gatgaagcgt tggtcaatag aaacttaaat
       61 tttcgttccg atatttttga actaggtgca ggtgtagaat ttcattacat gccttttcgt
      121 ttggggcacg accgttacaa aggaactgca tatttattgg cagaattagc cgtttttaga
      181 atgaacccta aaacacacta caacggtgag tgggtttatt tgcgaccttt ggggaccgaa
      241 ggacaaggct ctaatcttaa tgctaaaaaa ccctacagct tgtttcaatt tgccattcct
      301 ttaggattgg gtgttaaatt ctcactaggt aaacgcatgt ccattggatt agaatatgga
      361 attcgcctaa cgttcaccga ttatttagac gatgttaaag cagattctta tgtggatcct
      421 gtagctttag cagaggccaa cgggccaatg tcggcagctc tttccaacag aagtttggac
      481 ggtaatcgat atggtaaacg tggcaccgca gcaaccaaag attggtattc attttttgga
      541 ctcacgctaa cattccgttt gggtgaccct caaaaatgcg cgtttatgga ataatggtgt
      601 tgaaagagtt cttttaaagc aacgccaatt ttttattttc cagttttttg taaaaaattg
      661 taaatggtgg ttacagccaa tataattagc aacaccacta aatcagaaaa atgatatgca
      721 acactaatga gcataagaat agcagaaaat gcaaccatca aaaattgctc ttttaagtag
      781 tttttccaaa ccgttttttc atcttgcttc aaaaacaatt gaggcaatat aaattccatt
      841 ttataggatg aataggccat taaaccaacc ataggcacta aaacaatcca ttgctgaatg
      901 cccatttctg tataagtcaa aaaagcaata atttcttttt gaaaatcaat ggaggggtgc
      961 aataaggcac taccaatatg aaacaatgcc acaattaatg caaaaaacat tatggcaaca
     1021 cttgcaaaag tggatgtagg gatttgtgtg ttttgggtta cttgcttaat ttttcgtgct
     1081 tttaaaaaca ctacaatcaa ggctgccaat aggtcgaggg tataaaacaa caagatgaaa
     1141 tatagcgacc aattccacca aaaataaccc agcaacggaa tggctaaatt gccaattaat
     1201 tggatatata cctgttgttt ttgcattgat taacggtcaa gtttgttgta tgcatctatt
     1261 gataattgga atttatcgtc gtatttcaat gccgacatgt attcttgttt tgcctcttga
     1321 atcaatcctt ttttctcata acacattcca cggttgtgat atgcttcaac atagtttttc
     1381 atagtgttga tggcttctga gtaaaaatag atggcgctat ccacttggtt ttctaaattt
     1441 tgttttaaat aacccaagtg aaaataacct cttgcacggt aatatttttc tttttcttgt
     1501 tccattttgc ggtaatatac ttttgcgtcg tcgtattttt caaacgattc taaagcataa
     1561 gcataggagt acaaaaattc gggattggtt ttgttcaact catacgctgt ttggtaatat
     1621 tccaagcaaa ttgggttgtt ttcacggtga tacaatgctg ctaaatgaaa ataggcttcg
     1681 taaaaattag ggtcttgctg aatggctgtt tcatacgaag attttgccaa tgaataattt
     1741 tctaagtcaa catacaacga acctttgagt gcataagctt ctcttttctt tttatctatt
     1801 ttcaacgctt tgttcacata tttaaaggca ttgtccacgt cttgcatgta acgataagtt
     1861 gctgccaatg ctaccaatgc atccgtattt tctggttgct tgaacaatac aaattgaaaa
     1921 tggcgttgtg cgtttaaaac ttcttcaacg gttctgtttt ctttattgtt caacgcttgt
     1981 gcgtataaca tacgggtatc gggactcaaa ctatccaaac gaaaagcttt tgcaccatct
     2041 gccaacgcat caaaaaaacg ataatctttc aacgcttcat ttcctctaaa aagcaataac
     2101 ggaatactgt cgggataaag tgccaataaa ctatcaatat tagaagttgt tggttgaaca
     2161 gctatatttt tagttgtttt accaccacaa gcactgatta aaacgatgaa aagagaaaga
     2221 aaaaagaatt tttttatcat tgaatagaaa ttaacgttat caaaactaca ttttatttag
     2281 cacttcaaaa atagttgttt attttatgta ttcaacgggt tgttttgact tttgaacggt
     2341 tgttttttac tatttctgtg caaaacgata caaaacagct gccaccacac taaaaattac
     2401 attgggcatc cacgctgcta tcatggttgg gaaaccaaca ttttcggctg ccactgccat
     2461 aattttcatt gcaaaaataa agattaaaac aataatcaat cctatggcaa tattaacacc
     2521 aattccgcct ctttttttct cactggaaac ggcaattcca ataatggtga gaatg
//
LOCUS       DAOQVQ010000357         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48476_length_2574_cov_4.075427, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000357 DAOQVQ010000000
VERSION     DAOQVQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48476_length_2574_cov_4.075427"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..741
                     /locus_tag="PKN22_09550"
     CDS             301..741
                     /locus_tag="PKN22_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNS42990.1"
                     /translation="MEIENYIHQLPEEKQAVFLKLRKLVLENIPTGFEEGFEYGMVAY
                     VVPLSTYPKGYHCKANTPLPFISLAAQKNHFSFSHMGLYADEQLMEWFKNEFTKRYTT
                     KLDIGKSCMRFKKESDIPFELLAELLQKVTPTQWIEQYERVFRK"
     gene            complement(731..982)
                     /locus_tag="PKN22_09555"
     CDS             complement(731..982)
                     /locus_tag="PKN22_09555"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42991.1"
                     /translation="MKLNDTAMEGVYTLELSQFDFAINKILIFDTFGNQIKNILNPQN
                     QQTIDLSQHPNGIYHVSVIFDSEKAPIRLKIFKDSKTTS"
     gene            complement(1047..1787)
                     /locus_tag="PKN22_09560"
     CDS             complement(1047..1787)
                     /locus_tag="PKN22_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNS42992.1"
                     /translation="MKRNSLFKYSLIAFFVGFAVCNSFGQYVASGKITYERKTNLLKI
                     DNLPPFLKSIAEEKKYNIDNFVLTFNSDGSSFVPVASNAPASPADMLSIKNIVHCNRL
                     TGDRMTLLDIMGNKVYIHDTINKIKWTMTDNTRNLAGYECKMAIYKKDDSTRLYAWYS
                     EELVPAVGPETFSGLPGTILGLATEDGGVVYFAKKVEITPVTKEDLSFDPGKIKVYSK
                     NQFIAEAIKQMGNNPMVKGMLDGFFRWY"
     gene            complement(1765..>2574)
                     /locus_tag="PKN22_09565"
     CDS             complement(1765..>2574)
                     /locus_tag="PKN22_09565"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS42993.1"
                     /translation="PNRIKVGNPNLRPNYSHELNAWFNSWNPMNSGFIWAGMNASYTN
                     NDFATSTTYDAFGRMNIQTVNVDGNFNSNIYGGGGIPLKGRTIQLHPNLLAGYSHFTN
                     FINNQKNVTDNWLLGGGGEFKFRWDSLEFNFGGRYDWNSPKSSLNSASSTPYGVQNYT
                     FSIDWRLPLGFQIKTDARYTRNDRLSQGYSLNYFIWNASVSKNFLKTQNLNLAIEAND
                     ILNQSINAGRTVSGNMITDNRTKIITRYFLLRLTYRFNNFKTTEHEEKFPF"
BASE COUNT          860 a          466 c          410 g          838 t
ORIGIN      
        1 aatggaaaaa ttattgttta actcagaagt tgcatttgtg acttgaattc agcaaataaa
       61 attaaaacta aaagaaccaa ttccagaagt taccttaatt tgataccaca attcagatgg
      121 attttgtaac caaactttta agcgaggata ataataaata agtggaataa ttactaacaa
      181 aagtaacatc aacccacgaa taaaatatcg tattattttt cttttcacct aggtaaagat
      241 atattaaatt tgactagttt acttaactta ttgatgaata gccctatctt tgaataaaaa
      301 atggaaatag aaaactacat tcaccagtta cccgaagaaa aacaagccgt atttttaaaa
      361 ttacgcaaac ttgttttaga aaacattccc accggttttg aggaaggttt tgaatacggc
      421 atggttgctt atgttgttcc actttctact tatccaaaag gctatcattg caaagcaaac
      481 acacctttac cttttattag tttggctgct cagaaaaatc atttttcttt ttctcacatg
      541 ggactatatg ccgatgagca attaatggaa tggtttaaaa atgagtttac taaacgatac
      601 acaacaaaat tggatattgg taaaagttgt atgcgattta aaaaagaaag cgatattcca
      661 tttgaacttt tagctgaact tttgcaaaaa gttacaccaa cacaatggat agaacaatac
      721 gaacgcgttt ttaggaagta gttttactat ctttaaaaat cttcaaccgt attggtgctt
      781 tttcgctatc aaaaatgaca gaaacatgat aaatgccatt tgggtgttgg cttaaatcta
      841 ttgtttgttg attttgagga ttaagaatgt ttttaatctg attaccaaaa gtatcaaaaa
      901 tcaatatttt attgattgca aaatcaaatt gcgataattc taatgtataa actccttcca
      961 tagctgtgtc gtttagtttc atagcgtgaa atttgatgta aaattacagc tatcaaccca
     1021 gcaacacaat acgcaaccta gcgtatttaa taccacctaa aaaagccatc gagcatacct
     1081 tttaccatgg gattgttgcc catttgcttg attgcttcag ctatgaattg atttttagaa
     1141 tacactttta tttttcctgg gtcaaagctt aaatcttctt tggtaacggg agtaatttcc
     1201 acttttttag caaaatagac aacacctcca tcctcagtag ctaaacctaa aatggtgcca
     1261 ggtaagccac taaatgtttc tggaccaaca gctggaacta attcttcgga ataccaagca
     1321 tacaaacgtg ttgaatcgtc ttttttataa attgccattt tacattcata acccgccaaa
     1381 tttctggtat tatcagtcat ggtccacttt attttgttaa tcgtatcgtg gatgtaaacc
     1441 ttatttccca tgatgtctag caaggtcatt ctgtcgccag ttaaacgatt gcaatgaacg
     1501 atgtttttaa tagacaacat atccgccgga cttgcaggag cattacttgc aacgggcaca
     1561 aacgatgaac catcactgtt aaaagtcaac acaaaattat caatgttata tttcttttct
     1621 tcagcaatac ttttcaaaaa tggtggcaaa ttgtcaattt tcaacaaatt ggttttgcgc
     1681 tcgtaggtaa ttttccccga agcaacgtat tgcccaaatg aattgcaaac agcaaatcca
     1741 acaaaaaatg ctattaaact atatttaaaa agggaatttc tcttcatgtt ctgtagtttt
     1801 aaaattattg aaacggtaag ttaaacgcaa taagaaataa cgggtaataa tcttggtgcg
     1861 attatctgtt atcatattgc ctgaaaccgt acgaccagca ttaatacttt gatttaatat
     1921 atcattagcc tctatagcaa ggtttaagtt ttgtgttttt aagaagtttt tactcaccga
     1981 tgcattccaa ataaaataat tcaaactgta tccttgagaa aggcgatcgt ttctagtata
     2041 acgtgcatcg gttttgattt gaaagcccaa cggcaaacgc caatctatac tgaaagtgta
     2101 gttttgtaca ccataaggag tgctagatgc actattaagt gaactttttg gactgttcca
     2161 gtcgtatctt ccaccaaaat taaactccaa tgaatcccat ctaaatttga attcaccacc
     2221 accaccaagc aaccagttat cggttacgtt tttctggtta ttaataaaat tggtaaaatg
     2281 gctataaccc gctaacaaat taggatgcaa ctgaatggtg cgccctttta atggaattcc
     2341 accaccacca taaatattac tgttgaagtt gccatccacg ttaaccgttt gaatgttcat
     2401 tctaccaaaa gcatcatagg ttgtggatgt tgcaaaatca ttgttggtgt atgaagcgtt
     2461 cattcccgcc caaataaaac cactgttcat tgggttccac gaattgaacc atgcattcaa
     2521 ctcatgggaa taatttggac gcaaattggg gttacctact ttaattctgt ttgg
//
LOCUS       DAOQVQ010000358         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48640_length_2569_cov_2.615752, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000358 DAOQVQ010000000
VERSION     DAOQVQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48640_length_2569_cov_2.615752"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="glyA"
                     /locus_tag="PKN22_09570"
     CDS             complement(<1..867)
                     /gene="glyA"
                     /locus_tag="PKN22_09570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNS42994.1"
                     /translation="MAKDNEIFNLIEEERQRQINGIELIASENFVSDEVMQAMGSVLT
                     NKYAEGYPGKRYYGGCQVVDKVEQLAIDRLCQLFGATWANVQPHSGSQANVSVFSACI
                     QPGDKILGFDLSHGGHLTHGSPVNFSGKLYQTSFYGVNKETELVDYDMMEEVARKEKP
                     KMIICGASAYSRDWDYARIRKIADEVGALVLADISHPAGLIATGLLSDPFDHCHIVTS
                     TTHKTLRGPRGGVIMMRNNFENPFGLKTPKGELRMMSSLLDSGVFPGMQGGPLEHVIA
                     AKAIAFEEALSDD"
     gene            complement(869..1792)
                     /locus_tag="PKN22_09575"
     CDS             complement(869..1792)
                     /locus_tag="PKN22_09575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS42995.1"
                     /translation="MNILITGGAGFIGSNLTKRLVEEGNTVTVLDNLLRGNKIDSTTF
                     EKIKLIKGDVRDFDTVLNASKGCDIIIHFAAILGVDIVADNPVETMDTEVIGTRNVVL
                     AAQINNIKKIMYASTSGIYGHSAIESALTEEVLVDPRTSYAMAKRYNEIYLASHHEEK
                     GIDAIAVRFFNVYGYNQDNRMVVPKFFEQALKNAPITVFGTGKQTRDFTYIDDTVEAC
                     VRLLKVKGFHIVNVANEHEWCIDDVANEIKAVTNSNSPIQLIDAPKKRYDYEVERRVG
                     SSDKLFELTGYKPETSLNEGLKKIYKINYNK"
     gene            1946..>2569
                     /locus_tag="PKN22_09580"
     CDS             1946..>2569
                     /locus_tag="PKN22_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS42996.1"
                     /translation="MELLQNFINGEYTQPIDKQYIDNFEPATGNVYSQIPNSNEKDVE
                     IAVKAAQEAFPIWSNYSIEKRSKILMKVADGIRKRMDQFIAAESKDNGKPVSLAAHVD
                     IPRAISNFEFYATGIQHFASNSHYMVGVGVNYTLRKPIGVVGCISPWNLPLYLFSWKI
                     APALAAGNCVIAKPSEVTPYTAYLLGEVIKESGMPNGVLNILHGLGSS"
BASE COUNT          789 a          511 c          432 g          837 t
ORIGIN      
        1 gtcatcggat agcgcctctt caaaggcaat ggctttggct gcaatcacat gctcgagtgg
       61 cccaccttgc atgcctggaa atacaccgct atccagtaat gatgacatca tgcgcaattc
      121 cccttttggt gtttttaaac caaatggatt ttcaaaatta ttacgcatca tgatgacacc
      181 accacgagga cctctcaatg ttttgtgcgt tgttgatgta acaatgtggc aatgatcaaa
      241 cggatcgctc aacaatcctg tagcaattaa accagcaggg tgagaaatat cggctaaaac
      301 caaagcaccc acttcatcgg caattttacg tatgcgtgcg taatcccaat cgcggctgta
      361 ggcagaagct ccacaaataa tcatttttgg tttttcttta cgtgccactt cttccatcat
      421 gtcgtaatcc accaattcag tttctttgtt aacaccatag aaagatgttt ggtacaattt
      481 acctgaaaaa ttcactggag aaccgtgtgt taagtgccct ccatgtgaca aatcgaaacc
      541 taagatttta tctcctggtt gaatacacgc agaaaaaaca gaaacattgg cttgagaacc
      601 agaatgaggc tgaacatttg cccatgtagc accaaataat tggcacaaac gatcgatggc
      661 taattgctca actttatcca ccacttgaca tccaccataa taacgttttc caggatatcc
      721 ttccgcatat ttattggtta aaacgctacc cattgcttgc attacttcgt cgcttacaaa
      781 gttttctgat gcaatcaact cgataccatt aatctgacgc tgtctttctt cttcaatcag
      841 gttgaagatt tcattatctt ttgccatatt atttgttgta atttatttta taaattttct
      901 ttaaaccttc atttaagcta gtttcaggtt tgtagccagt caattcaaat aatttatcag
      961 agcttccaac acgtctttcc acctcgtaat cgtaacgttt tttaggtgca tcaattaatt
     1021 gaataggaga gttggagttg gttacagcct taatttcatt agcaacatca tcaatacacc
     1081 attcgtgttc gttggcaacg tttacaatgt gaaatccttt tacttttaac aaacgcacac
     1141 atgcctcaac agtgtcatca atataggtga aatcgcgtgt ttgttttcct gtgccaaaaa
     1201 ctgtaattgg tgcatttttc aacgcttgtt caaagaactt tggaactacc attctgttat
     1261 cttggttgta tccatacaca ttgaaaaaac gaacagcaat agcatcaatt cctttttctt
     1321 catgatgcga tgctaaatag atttcattgt agcgttttgc cattgcataa gaggttctag
     1381 gatctaccaa cacttcttct gttaatgcac tttcaatggc agaatgtcca taaatcccac
     1441 ttgttgatgc atacattatt tttttaatgt tattgatttg tgccgccaaa actacgttgc
     1501 gtgttcctat gacttctgta tccatcgttt caacaggatt atctgccaca atatcaactc
     1561 ctaaaattgc tgcaaaatga atgataatat cacacccttt acttgcgttt aataccgtgt
     1621 cgaaatcacg aacatcacct tttattaatt tgattttttc aaaagtagtt gaatcaatct
     1681 tgtttcctct aagtaagtta tccaatacag taacggtatt accttcttca accaaacgct
     1741 ttgtaaggtt acttccaata aaaccagctc ctccagttat aagtatattc atatggttat
     1801 attccggtta tttttgggtc aaatatacta aaataataaa gttttagaag tgtatcaaac
     1861 cgattaaatg caaatatttt aatggaatac tttcaatatg ctttgaatcc ctatctttgt
     1921 aacaaataaa aagtaaagac aaaccatgga actattgcaa aattttatca acggagaata
     1981 tacacaacca attgataaac aatatattga taattttgaa ccagcaactg ggaatgttta
     2041 ttcacaaatc cctaattcaa atgaaaaaga tgtagaaatt gcagtaaaag cagcgcaaga
     2101 agcttttcca atttggtcga attacagcat agaaaaacgc agtaaaatat tgatgaaagt
     2161 tgcagatggt attcgcaaaa gaatggatca atttattgca gcagaatcaa aagataacgg
     2221 aaaaccagtg tcattagcag cacatgttga tattcctcgt gctatttcaa attttgagtt
     2281 ttatgccaca ggtattcagc attttgccag caattcacat tatatggttg gagtaggggt
     2341 taactatact ctacgcaagc caattggtgt ggtaggttgt atatctccgt ggaatttgcc
     2401 gttgtattta ttttcatgga aaattgctcc tgcattggct gccggcaact gtgtgattgc
     2461 aaaaccatca gaagttacgc cttatacagc ctatttatta ggtgaagtta ttaaagaatc
     2521 gggaatgccc aatggtgttt taaacatctt gcatggttta ggttcttcg
//
LOCUS       DAOQVQ010000359         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48694_length_2567_cov_2.810111, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000359 DAOQVQ010000000
VERSION     DAOQVQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48694_length_2567_cov_2.810111"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKN22_09585"
     CDS             <1..272
                     /locus_tag="PKN22_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNS42997.1"
                     /translation="AVLNLPLGNTDSISPGVMINLLGEEGFSGPAKYLGIKDAMQQKG
                     VFIHLYGKTTTKPHRKMGHITIALNSLQDAKLTAKKLINSVKVIA"
     gene            308..>2567
                     /locus_tag="PKN22_09590"
     CDS             308..>2567
                     /locus_tag="PKN22_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS42998.1"
                     /translation="MKTFLHYITLLLITLKSSIYLLYGQEYHYSNYTHKDGLQNVSIT
                     TLTENSDGGIIIGTEGSGIIEFDGYEFKSLIQQNINNNHQVSAVEINKNAIYFASRYR
                     GIYKIDAQKSLHEIVSSNSVKNFISLALFNNELLYINNNELYRYDIDKKTQSKIYSFK
                     KDISIYQTIKTPNGLIYLTTIGNFIYSNKSKKIVDLNTYFNTTDEGIKNVSFGYYANG
                     LLYFFNFKDNISLLVLLNEDGIYNSKIKLLTGSNFDSEVISATYNKVKKCFTAITKLG
                     RIYEISNGNVEKKIQNTSLQNIAAKTIISDYYGDYWIGSYFTGLIRVSKQPFTKIEYL
                     PNYLSERIGLIDFTQDNKNIIYSTMDHETFVGNYLNGPLKKYDFQSYSSTYFKNTLLI
                     GTNNGVYEYIEQTNELKKLPILPEGPINHIVVHNTDLYIAVRKKGLYKFDKNYHLIQH
                     FEPTHSAPSIIYTSQIDGDIIYFGTSQGIKKLDTKNNLFSSIDTKGLGEYCGLSITDV
                     FGTKWFALDFGLLGISKKGEKYVINDPEILSSFLFYSLNTDSFGNLYIGTNNGINVLK
                     VNDEGKVLSHQIYTAKNGFGGYEPHMRSSFQTPNFAFIGTLEGIWKINFNLLQGLTPP
                     QTPIINIKESIDPNNPSTISIQFISKNPKIKSVYYTYRIVNNNSQWSALSLENEFNLS
                     DLKNGDYSIEVKATYDGVYFSDIGVKSFHINQTVLQSKILIYLLISFIVIVNILFFLK
                     AKKNDPYEVLSTS"
BASE COUNT          968 a          402 c          370 g          827 t
ORIGIN      
        1 gggcagttct caatttacct ttaggtaata cagacagtat ctctccaggt gtaatgatca
       61 atcttttagg tgaagaagga ttttctggtc cagccaaata tcttggaatt aaagatgcaa
      121 tgcaacaaaa aggagtgttt attcacttat atggtaaaac tacaacaaaa ccgcatcgaa
      181 aaatgggaca catcactatt gccttgaata gtctacaaga tgctaaatta acagccaaaa
      241 aactaataaa ttcagttaaa gtaattgctt agtttaaagt tattattttt ttcttatctt
      301 ggtagtcatg aaaacattct tgcactatat cactttattg ttaatcacct taaaatcaag
      361 tatatatcta ttatacggtc aagaatatca ttacagtaat tatacccata aagatggatt
      421 gcaaaatgtt tcaattacca ctttaaccga aaattctgat gggggtatca taataggaac
      481 tgaaggaagt ggaattattg agtttgatgg ctatgaattt aaaagtttaa ttcaacaaaa
      541 cattaacaac aaccaccaag taagtgctgt tgaaattaat aaaaatgcaa tctactttgc
      601 atcaagatac agaggaattt ataaaattga tgcacaaaaa tcattgcacg aaattgtatc
      661 ttccaattct gtaaaaaact ttattagtct tgctctgttc aacaatgaat tgctgtatat
      721 caacaacaat gagctgtatc ggtatgatat agataaaaaa acacaatcaa agatttatag
      781 ttttaaaaag gacatttcaa tttatcaaac cattaaaact ccaaatggtt taatctattt
      841 aactacaatt ggaaatttca tttattccaa taaatctaaa aaaatagttg acttaaacac
      901 ctattttaat actactgatg agggaataaa aaatgtcagt tttggttatt atgcaaatgg
      961 actactctat ttctttaatt tcaaagataa catatcttta ttagttttac ttaatgagga
     1021 tggaatttac aattcaaaaa ttaagctgtt aactggtagt aatttcgatt cagaagtcat
     1081 tagcgcaacc tataacaaag taaaaaaatg ttttacagcg attaccaaac ttggtcgcat
     1141 ctacgagatt tctaacggga atgtcgaaaa aaaaatccag aatacaagtt tacaaaacat
     1201 tgctgcaaaa acaatcattt ctgattatta tggcgattat tggataggat cttattttac
     1261 agggcttatt cgagtgagca aacaaccttt tacaaaaatt gaatacctcc ctaactattt
     1321 gagtgaacgc attgggttaa ttgattttac ccaagacaac aaaaacatca tttattccac
     1381 tatggatcat gaaacatttg ttggtaatta tctcaatggt cctttaaaaa aatatgattt
     1441 tcaaagttac tcctctacat attttaaaaa cactttatta attggaacta acaatggtgt
     1501 ttatgaatac attgagcaaa caaatgaatt aaaaaaacta cctattctac ccgaagggcc
     1561 tattaaccac attgttgttc ataataccga tttgtatatc gctgttagaa aaaaagggtt
     1621 gtacaagttt gataaaaact atcatctaat acagcatttc gaacctacac attctgcacc
     1681 ttcaatcatc tatactagcc aaatagatgg agatataatt tattttggaa cgtctcaagg
     1741 aattaaaaaa cttgacacaa aaaacaattt gtttagttca attgacacaa aaggactagg
     1801 agaatattgt ggactgtcca taactgatgt atttggaaca aagtggtttg cattagattt
     1861 tggattactt ggcatttcaa aaaaaggtga aaaatatgta atcaacgacc ctgaaatttt
     1921 gtcaagtttt ctattttatt ctttaaacac agatagtttt ggcaacttat acataggtac
     1981 caacaacggt atcaatgtgt taaaagtaaa cgatgaaggc aaagtacttt cacatcaaat
     2041 ttacacggca aaaaatggat ttggaggata tgagcctcac atgcgttcct cttttcaaac
     2101 acccaacttt gcttttattg gaaccttaga aggaatatgg aagattaact tcaatttatt
     2161 gcaaggatta actccacctc aaacgccgat aatcaatatc aaagaaagta tagaccccaa
     2221 caatccatcc actatatcca ttcagtttat atcaaaaaac cccaaaataa aaagcgtata
     2281 ttatacgtat cgtattgtta ataataactc acagtggagt gcattatccc tagaaaatga
     2341 atttaattta tccgatttaa aaaatggaga ttacagcatt gaagtaaaag caacttatga
     2401 cggtgtttat tttagtgata ttggcgttaa atcttttcat ataaaccaaa ctgttttaca
     2461 aagtaagata ttaatttatt tacttatatc gtttatcgta attgtcaaca ttcttttttt
     2521 cctaaaagcc aagaaaaatg atccatacga agtattaagt acaagtg
//
LOCUS       DAOQVQ010000360         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48747_length_2565_cov_3.475697, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000360 DAOQVQ010000000
VERSION     DAOQVQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48747_length_2565_cov_3.475697"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..465)
                     /gene="gatC"
                     /locus_tag="PKN22_09595"
     CDS             complement(175..465)
                     /gene="gatC"
                     /locus_tag="PKN22_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNS42999.1"
                     /translation="MKITNEIIDHIAHLSRLEFEGSEKDAIRQDMENIISFMDVLQEV
                     DTNNVEPLIFMNDEINHLREDIPTVTLTQQEILKNAPKKDSDFFRIPKVLDK"
     gene            530..1003
                     /locus_tag="PKN22_09600"
     CDS             530..1003
                     /locus_tag="PKN22_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNS43000.1"
                     /translation="MKTTKIERLLREYAESHQNKTNKLIHWVCVPSIFFSIFGMIRCL
                     PFPNALQEFSSYINWASLVLILVLFYYLTLSFKLFVGFLFWALFVLFANEFIFVQLGN
                     SGLLLFSIGVFVIAWIGQFIGHGIEGKKPSFFKDLQFLLIGPAWLMNFILKVVQL"
     gene            complement(1059..2117)
                     /locus_tag="PKN22_09605"
     CDS             complement(1059..2117)
                     /locus_tag="PKN22_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNS43001.1"
                     /translation="MKKNMIKKRLQSLLTLALIGGSTILLYSRCSSPEVKDEVVVDDS
                     ADKALLQKANSIFKSISSIPQDDIAMNKILLGKKLYHDKSLSKDQTISCASCHNLNTY
                     GVDNLAFSPGDTKELGGRNSPSSVYAFLHSVQFWDGRAKDVEEQAGGPILNPVEHNVP
                     NQAFLEKRLRENKEYQRLFKEVYPNEAQPITFANITNAIGAFERQLAPESRFDKWLDG
                     DNTALNAQEKKGLQAFMDNACITCHMGPAVGGGLMQKFGLYGNYWEKTNSKKIDNGVY
                     DLTKKESDKYIFKVPSLRNVEKTGPYFHDGSVTKLEDAVSMMAELQLGNKLSDEDVKN
                     ITAFLKSMTAEVDAKYIE"
BASE COUNT          810 a          417 c          408 g          930 t
ORIGIN      
        1 acgaaacaag ctaaagctgc aaaagagagt tgtgaagaag ttggagtaac ttttagtgtt
       61 gttaaaacca tgtaacttta agctagtttt tgtaaatact tttttaaaga aaaaaattaa
      121 atatggggat tctgatcagt actcccctat ttttttgtca taaaataaga acaattactt
      181 atctaatact ttaggaattc taaagaaatc agaatctttt ttaggtgcat ttttcagaat
      241 ttcttgctgt gttaatgtca ctgtaggaat atcttcacgc aaatggttga tttcgtcgtt
      301 cataaaaatc aacggctcca cattgttggt atccacttct tgcaacacat ccataaatga
      361 aatgatgttt tccatatctt gacgaatagc atctttttca gaaccttcaa attctaaacg
      421 agacaaatgc gcaatgtgat caataatttc gttggtaatt ttcatgtttt taaaatcagt
      481 tgacaaatgt agtgattttt tttaatttag tatgtcaaaa tcccgaatca tgaaaacaac
      541 aaaaatagaa cgtttattgc gcgagtatgc cgaaagtcat caaaataaaa ccaataaatt
      601 gattcattgg gtatgcgtac caagcatttt tttctccatt tttggaatga tacgttgcct
      661 gccatttcct aatgctctgc aagaattttc aagctacatc aattgggcat cactggtgtt
      721 gattttagtg cttttttatt atctaacact ttcttttaaa ttatttgttg gttttttatt
      781 ttgggcactt tttgtgttat ttgccaacga atttattttt gtacaattgg gaaatagcgg
      841 tttattgttg ttttctatag gtgtttttgt aatagcttgg attggtcagt ttattggaca
      901 cggaatagaa ggtaaaaaac cctctttttt caaagatttg cagtttttgt tgattggtcc
      961 cgcttggttg atgaatttta ttttaaaggt agtgcagcta taaaaaaaga gattgcccaa
     1021 gttgaacaat ctcttttgat aattttgctt ttagaatgct attcgatgta ttttgcatca
     1081 acttctgctg tcattgattt taagaatgca gtaatatttt ttacatcttc atctgataat
     1141 ttattgccca attgcaattc ggccatcatg cttacagcgt cttccaattt agtaacagag
     1201 ccatcgtgga aataaggtcc tgttttttca acgtttctta aacttggaac tttaaagatg
     1261 tacttatcgc tttctttttt ggttaaatca taaacaccat tgtcgatctt cttactgtta
     1321 gttttttccc agtaattacc atataaacca aatttttgca tcaagccacc accaacagct
     1381 ggtcccatgt gacaagtaat acatgcatta tccataaatg cttgtaaacc tttcttttct
     1441 tgtgcgttta aagccgtatt atcgccatcc aaccatttat caaatctact ttcaggtgcc
     1501 aattgacgtt caaaagcacc aattgcgttt gtaatgtttg caaaggtgat aggttgcgcc
     1561 tcgttaggat acacttcttt aaacaaacgt tgatattctt tgttttcgcg caatcttttt
     1621 tctaaaaatg cttggtttgg aacgttgtgt tcaactgggt ttagaattgg tccacctgct
     1681 tgttcttcca catcttttgc tctaccatcc caaaattgaa ccgagtgcaa aaaggcataa
     1741 acagatgaag gtgaatttct gcctcctaat tctttggtat cacctggaga aaatgctaaa
     1801 ttatcaacac cataagtgtt taagttgtgg catgaagcac aactaatggt ctgatctttc
     1861 gataaacttt tatcgtggta aagtttttta cctaataaaa ttttgttcat tgcaatatca
     1921 tcttgtggaa ttgatgaaat tgatttaaaa atagagtttg ctttttgtaa taaagctttg
     1981 tcagcgctgt cgtcaacaac tacttcatct ttcacttcag gtgagctaca tctagagtac
     2041 aaaagaattg tagaaccacc aataagtgcc aatgtcaata aagattgtaa gcgtttttta
     2101 atcatatttt ttttcatagc caattttttt tacaaatgca caacagttta agtgacttca
     2161 atcataaaaa atattgatac acatatagat ttttattatg gctagattag agagtaaaca
     2221 ggtgttataa tgttaaaata ttccatattt acataaacaa tattccaaat atacataaga
     2281 aaaaatccaa taatgcatag taaaaattcc acatatacat aagataaaat ccaatattac
     2341 ataaaaaata ttccaaatat gcataaatat tattccaaaa ttgcatagat ttgtagtatg
     2401 ataaatagaa tgattgtaaa aaaagcaaac gatatggcag gaaaatttcc tgttattgtt
     2461 gttactggtc cacggcaggc cggtaagagc acgtttgcaa aattagcatt ccctacttat
     2521 cgttatgtgt cgttggaaaa ccttgataat cgtcaatttg cacaa
//
LOCUS       DAOQVQ010000361         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_48847_length_2562_cov_3.187076, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000361 DAOQVQ010000000
VERSION     DAOQVQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48847_length_2562_cov_3.187076"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..393)
                     /locus_tag="PKN22_09610"
     CDS             complement(40..393)
                     /locus_tag="PKN22_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43002.1"
                     /translation="MKYFVLLFVLCAILNSKKQSQQTIFETDRFLYWQNGTEINFDDF
                     TLPIDSSGIKLCEKYGTNSLANIQIHSILDYPKNKRSLKTLDPKIYFAPSFCKDCSLL
                     LQRDSSELKLAVYIN"
     gene            complement(610..924)
                     /locus_tag="PKN22_09615"
     CDS             complement(610..924)
                     /locus_tag="PKN22_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43003.1"
                     /translation="MKFEFTGQLKILIDKAQSGNSNDVDYIMDKLTVDSTFAITRYVD
                     FALSLVEKNEGKERIKYYLFNGTLIQRNYASLFLNRLGEWKSVKKAFEQGLIDEIQSY
                     SR"
     gene            complement(929..1771)
                     /locus_tag="PKN22_09620"
     CDS             complement(929..1771)
                     /locus_tag="PKN22_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS43004.1"
                     /translation="MAKTLIYKSDEGKKQVLSYYETLLTQWHQPFRQITLETTFGNTF
                     IIESGLKESPTILFLHGSGSNSAMWLGDAMILSETYHVLAIDIIGECGKSSENRPEFK
                     NGNYSGWISEIIEKLALNRISIIACSLGGWIALDFSIKHPERIEKLVLMATAGITQVK
                     LKTIFWIIITSMMGTWGFNKLNRMVYGKLTIDNKALEFASLVKENYKPRTDVLPVLTN
                     ESLRQIKAPTLFIGGENDCFYDSRKTASRLKENMENVKCLVLKDTGHVLTNQTNNILQ
                     FLNS"
     gene            complement(1840..2397)
                     /locus_tag="PKN22_09625"
     CDS             complement(1840..2397)
                     /locus_tag="PKN22_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS43005.1"
                     /translation="MQQLIDYIERFVRLDSEAISALENLAEIETFKKNQFILEPGQRC
                     NKIWFLKSGMVRKFHLCNGKEITTWIHTENDTFTSLQSYAQNTPSDEYLQVCEDTEAI
                     GITKVNSEKLAKYPPFIVFSNNLMEREFVNIDKHTKALNQRDAKGKYEYLKLIAPEII
                     KRAQVGYIASIIGVSRETLSRIRKG"
BASE COUNT          788 a          446 c          390 g          938 t
ORIGIN      
        1 ataagtggta tacttttcaa cgagaatgtg gtatactttt caattaatat atacagctaa
       61 ttttagttcg gatgaatctc tttgaagcag taatgaacaa tctttacaaa aacttggtgc
      121 gaaataaatt ttagggtcta atgtttttaa actccttttg ttctttggat agtcaagaat
      181 agaatgaatt tgaatatttg ctagagaatt agtaccatat ttttcgcaaa gtttaattcc
      241 agatgaatca attggtaagg tgaaatcatc gaaatttatt tctgtcccgt tttgccaata
      301 tagaaatctg tcagtttcga aaattgtttg ttgtgattgt ttttttgaat ttaagattgc
      361 gcacaagaca aataataata cgaaatattt catggtatgc gattttaatg taagtagtta
      421 taattctgag cacaaatgcc acataacggt tgcgtgtatg aaacgtttgg catttcgaag
      481 ctatttccta tcaagttaca aactactatt gatacaagct atgccgctcg ataaaccact
      541 gaatgccaaa tgttttatac acgatgttgt gcccagtact ttattgtttt caatgcaata
      601 taatttaaat caacgagaat atgattgaat ctcgtcaatt aatccttgct cgaatgcttt
      661 ctttactgat ttccattctc caagtcggtt taagaataaa cttgcgtagt ttctttgaat
      721 taaagtgccg ttaaacaaat aatatttaat tcgctctttc ccttcatttt tttcaactaa
      781 acttaatgca aaatcaacat atcgagttat cgcaaaggtt gaatccacgg ttagtttgtc
      841 cattatataa tcaacatcat ttgaattgcc actttgagct ttatcaatca atatctttag
      901 ttgtcctgta aattcaaatt tcaccttttc aggaatttag gaattgtaaa atattattgg
      961 tttgatttgt taaaacatgt cccgtatctt ttagtactaa acatttaaca ttttccatat
     1021 tttcttttaa tcgggaagcc gtttttcgtg aatcgtaaaa acaatcattt tcacctccga
     1081 taaacaatgt tggagctttg atttgccgca atgattcatt ggttaaaacg ggtaacacat
     1141 ctgttcgggg tttataattc tcttttacta atgaagcaaa ctccagtgct ttattgtcaa
     1201 tagtaagttt tccatacacc atcctgttta gtttattgaa accccaagta cccatcattg
     1261 atgtaatgat tatccaaaaa atggttttaa gcttaacttg tgttattcct gctgttgcca
     1321 ttaatacaag cttttcaatc cgttcgggat gtttaattga aaaatccaac gcaatccatc
     1381 caccaagcga acaagctata atagaaatac gatttaatgc aagtttttca attatttcag
     1441 aaatccagcc agaataattt ccatttttaa attcaggtct gttttctgaa ctttttccac
     1501 actctccaat tatatcaatt gccaatacat ggtaagtttc tgaaagtatc attgcatctc
     1561 caagccacat ggcagaattt gaacctgaac catgcagaaa taatattgta ggagattctt
     1621 ttaaaccgct ttcaataata aatgtattac caaatgttgt ttcaagagta atttgtctaa
     1681 aaggctgatg ccattgtgtc aacaatgttt cataataact taaaacttgt tttttccctt
     1741 catctgattt ataaatcaat gtctttgcca tattgtaatt tttttaattg gcaaagttcg
     1801 gtgagaaaaa tttaagttct tttgatatag gtcacaaaat cacccttttc taatcctgct
     1861 caaggtttct cttgatacac caattattga agcaatatag cctacttgcg cccgtttaat
     1921 aatttcagga gcaatcaatt tcagatactc gtatttccct tttgcatctc tttgattcag
     1981 agcttttgtg tgtttgtcta tatttacaaa ttcacgctcc attaagttat tagaaaaaac
     2041 tataaatggt ggatacttag caagcttttc actgttaacc tttgtaatac caatcgcttc
     2101 agtatcttca catacttgta aatattcatc tgaaggagtg ttttgagcat aactttgtaa
     2161 agaggtaaat gtgtcatttt cagtatgaat ccaagtggta atttccttcc cgttacataa
     2221 atgaaatttt cgtaccattc cacttttcaa aaaccagatt ttattgcaac gttgtcctgg
     2281 ttcaagaata aactgatttt tcttgaatgt ctcaatttca gcaagatttt ctaaagccga
     2341 aattgcctct gaatccagtc ttacaaatcg ttctatataa tcaatcaatt gttgcatttt
     2401 cggtttttct tagtattggg cacaacggtt agcggtatga aacgttgggg gtttcggagc
     2461 tacttcacta tcaaggtaca acaaactttg atgcgagaga tgacgtttgc ataaccactt
     2521 aaaccccaat gttttatgac cgcgtgttgg caccagtaca tt
//
LOCUS       DAOQVQ010000362         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_49251_length_2548_cov_4.788608, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000362 DAOQVQ010000000
VERSION     DAOQVQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49251_length_2548_cov_4.788608"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKN22_09630"
     CDS             complement(<1..821)
                     /locus_tag="PKN22_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNS43006.1"
                     /translation="MYVIKRDGKKEAIKFDKITARIMKMCYGLDPIVSPEAVAMKVIE
                     GIYDGISTMDLDNLAADVASTRAIEHPDYALLASRIAISNLHKETKKSFSEVMTDLYT
                     AKDVKTDEATPLIAQDVYDIICNNKDLLDSTIIYDRDFKFDYLGFRILADHYLMRIDN
                     QIVERPQQLLMRLAIGIHKTNIPAVIKTYNYLSEGWMMHDITTLNNAGRPNAQLLSSY
                     SLSIDDSSVETIYATIKSAAQISNGAGELGVNISRVFASEIEKDQNPSLETNKILP"
     gene            1115..1849
                     /locus_tag="PKN22_09635"
     CDS             1115..1849
                     /locus_tag="PKN22_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43007.1"
                     /translation="MKTAPSGFGKFIATIANYWDGLLHLLFPSSCVVCKNELSRTEQV
                     CCSICYSELAFTHFEISKEPTEVDQLFWGRLPITSTYSLLYYEKTNNSKPILAALKYG
                     NRPDVGQYFGRIIGKTLCQHPLFSTVEVLIPVPIHTKKKYTRGYNQSEEIVKGITDVW
                     EIPFDFKVLARNKHTGSQTKLGRFSRWDNADGLFTPDESIKNYKHIALVDDVITTGST
                     LEAITNRIFAIAPDIKVSVISLAVTR"
     gene            1846..>2548
                     /locus_tag="PKN22_09640"
     CDS             1846..>2548
                     /locus_tag="PKN22_09640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS43008.1"
                     /translation="MKNFLIVGGGIAGCSLAYQLAQKGCQITVVDAGNNVSTKVAGGV
                     IIPLVFRRMTKSWRVDEFLPYAKQFYGELETASNQKILYPMALRRFFSSNQERNYWLT
                     RQNTPEFSTYMNEVNEEDETCYPYANKFGSGRVKECYRVDAEWMTTALLNHPQITVVN
                     QRFDYSALDATTATYQQQSYDCVVFCEGYESINNPWFSYLNVDPTKGEVLTVKTTGLS
                     NTEVLNRKCSAAPIGN"
BASE COUNT          797 a          448 c          500 g          803 t
ORIGIN      
        1 ggaagtattt tgtttgtttc taatgatgga ttttggtctt tttctatttc acttgcaaaa
       61 acgcgcgaaa tgttaacacc caattctcca gctccattag aaatttgtgc cgcagatttg
      121 atagtggcat aaatggtttc taccgaacta tcgtcgatgg aaagcgaata actggaaagc
      181 aattgagcgt tgggacgacc agcattgttc aatgtagtga tatcgtgcat catccaacct
      241 tctgacaaat aattataggt tttgattaca gccggaatgt ttgttttatg aattccaatc
      301 gccaatcgca tcaataattg ttgcggtcgt tccacgattt gattgtcaat gcgcattaaa
      361 taatgatcgg ccaatattct aaatcctaaa taatcaaatt taaaatcacg gtcgtatatg
      421 atggtagaat ccaacaaatc tttgttattg caaataatgt cgtaaacatc ttgagcaatg
      481 agtggagttg cttcgtccgt ttttacatct ttggctgtgt acaaatccgt cattacttcc
      541 gaaaatgatt ttttagtctc tttgtgcaag ttggaaatag caattcgaga ggctaataaa
      601 gcataatcgg gatgttcaat ggctctcgtc gatgccacat cagctgccaa attatccaaa
      661 tccattgttg atatgccgtc ataaatacct tcaattactt tcatggcaac ggcttctggt
      721 gaaacgatag gatccaatcc gtagcacatt ttcatgatgc gagcagtaat tttgtcaaat
      781 ttaattgctt ctttttttcc gtctcttttg attacgtaca tatcaatttt tggtgttatt
      841 atagtttttt taatagcttt ttctttggcg gtgctttttc agtaaattgt gcgagcttcc
      901 aaagtgggat acaaaaattg aaaaaataaa aaccaatcgc aattaaagaa attcacatct
      961 tccattgggt tttcaacact ggttttacaa tgcgtttatt ttaagcactt agacaggttt
     1021 taaacatcaa aataaagatt tccacataga gttttgaaaa gtgttaattc catccaaagt
     1081 ttcttatatt tgttagaact acaagaagaa atcgatgaaa actgcgccgt ctggttttgg
     1141 aaaatttatt gcaacgattg ccaactattg ggatggttta ctgcatttat tatttccttc
     1201 aagttgtgtt gtttgcaaaa atgaattaag cagaacagag caagtgtgtt gttctatctg
     1261 ttattctgaa ttggcgttca cccattttga aatttctaaa gaacctaccg aagtggatca
     1321 attgttttgg ggaagattgc caattacctc cacctattct ttactgtatt atgaaaaaac
     1381 aaataacagc aaacccattt tagcagccct taaatatggc aaccgaccag atgtgggaca
     1441 gtattttgga agaattattg gaaaaacact ctgtcagcat cccttatttt caaccgttga
     1501 ggtgttgatt cctgtgccca ttcataccaa gaaaaaatac accagaggct acaatcaaag
     1561 tgaggaaatt gtaaaaggaa taacggatgt atgggaaatt ccttttgatt ttaaggtatt
     1621 agcaagaaat aaacacacag gcagtcaaac aaaattgggg cggtttagta ggtgggacaa
     1681 tgccgatggt ttgtttactc ccgatgagag catcaaaaac tacaaacaca ttgccttggt
     1741 agatgatgtt attacaacag gttctacgct agaagccata acaaatcgta tatttgccat
     1801 agctcccgat atcaaagtga gcgtaattag tttagcagta acacgatgaa gaatttttta
     1861 attgttggag gcggaattgc aggttgctca ttggcctatc aattggcaca aaaaggatgt
     1921 caaattacag tagttgatgc aggaaataac gtgagcacaa aagtggcagg gggtgttata
     1981 attccacttg tttttagacg aatgactaaa agttggcgtg tagacgaatt tttgccttat
     2041 gcaaagcaat tttatggaga attagaaact gcaagcaatc aaaaaatttt atatccaatg
     2101 gcactgcgtc gttttttttc gtccaatcaa gaacgcaatt attggttgac ccgccaaaac
     2161 acacctgagt tttctacgta tatgaacgag gtaaatgagg aggatgaaac gtgttatccg
     2221 tatgccaata aatttggttc gggaagagta aaagaatgtt accgtgttga tgctgaatgg
     2281 atgaccactg ctcttttaaa ccacccccaa attacggttg taaaccaacg atttgattat
     2341 tctgcattgg atgcaacaac agcaacctat caacaacaat cgtatgattg tgttgttttt
     2401 tgcgaaggct atgaatccat caacaatccg tggtttagct atttgaatgt tgacccaacc
     2461 aaaggggagg tgttgacggt taaaacaaca ggtttatcca acacagaggt tttaaataga
     2521 aaatgctcgg ccgctcccat cggaaacg
//
LOCUS       DAOQVQ010000363         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_49304_length_2547_cov_2.393660, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000363 DAOQVQ010000000
VERSION     DAOQVQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49304_length_2547_cov_2.393660"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PKN22_09645"
     CDS             <1..1743
                     /locus_tag="PKN22_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43009.1"
                     /translation="TIGTSINHNDNDCSVNINANAVALISNSSLNDEAKFIDFNGQVI
                     YTEINSTGAVNWQPNFVMTGFNVQSDYNCVNGNMLNSFAMPNDVYKIICEQDTMYNPI
                     ILVQEPLNFTPCVYKSVEGFIYVDNNGDCINNSGDVGITYITPVINGNYIYDPFQNPF
                     SNGNANGNYKFFVQENHLIDYTVSIPSIYAFVFPASPCFQTSYSFTNLPQTGVDFPLQ
                     CADFDTHVQYYSTPARPAIPFTLSPRVFNIGCDAVSGTLKLKLDQNVTYNSTLSSHPA
                     DYINGDTLVWNFTNLNNLANNTPYFDQFLGGIHLTPSLSVNIGDTLWFDLSTSVATGD
                     VNPANNHLHFGIPIVNSFDPNIKEVSPKGVGIEGLIPMETEALTYTIHFQNTGTAEAI
                     NIFVLDTLTASIDPKSLQILANSHNMTPTWINDSVVKFNFPNIHLADSSSNEPESHGF
                     VRFKVNLKDNLPIGTIIKNKAAIYFDTNEPVITNFTTNKLANDLSVSKIEKLNDISVF
                     PNPAKDYVIIRFFEQINGTIELFDLTGKNVYTEEVKQISQKQIDVKQLQNGVYIYQIK
                     ANNGQRKSGKLIIN"
     gene            1916..2530
                     /locus_tag="PKN22_09650"
     CDS             1916..2530
                     /locus_tag="PKN22_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNS43010.1"
                     /translation="MKRLTVFSIRSFIGPFFVTFAIAMFILIMQFFWKYIDDLMGKGI
                     ETIYILQLLFYVSASLIPLALPLAMLLSSIMTLGNLAEFNELTALKSSGLSLYRILRP
                     LTAIVLILALSTFLFANYVIPVANYKWRSLIYDFQNTKIASIIVPGVYTKEIDGYAIK
                     ANKKNPDGSMEDITIHDKTKPNEFKTIKAAKGTLYKSDNGNIFH"
BASE COUNT          838 a          414 c          394 g          901 t
ORIGIN      
        1 actattggaa catctatcaa tcacaatgac aatgattgtt cggttaacat taatgccaat
       61 gcagtggctt tgatatcaaa ttcttcttta aatgatgaag ctaaatttat tgattttaat
      121 gggcaagtaa tttatactga aattaattca actggtgctg taaactggca accaaacttt
      181 gtgatgacag gatttaatgt acaatcggat tataattgtg ttaatggaaa tatgttgaat
      241 tcttttgcaa tgcctaatga tgtctataaa ataatttgtg aacaagatac tatgtataat
      301 ccaattattt tagtacaaga acccttgaat tttacaccat gtgtttacaa atctgtagaa
      361 ggttttatat atgttgataa caatggtgat tgtatcaata attcaggtga tgttggaata
      421 acctacataa cgccagttat taatgggaat tatatctacg acccttttca aaatccattt
      481 tctaatggaa atgcgaatgg aaattataaa ttttttgttc aagaaaatca tttaattgat
      541 tataccgtta gcataccatc catctatgct tttgtttttc cagctagtcc ttgttttcaa
      601 actagttatt cgtttacaaa tctaccacaa actggtgttg attttccttt gcaatgcgct
      661 gattttgata cacatgttca atattattct actccggcac gtcctgcaat tccattcaca
      721 ttatcacctc gagtttttaa tattggttgt gatgctgttt caggaacgtt aaaactaaaa
      781 ttagaccaaa atgttaccta taattccacg cttagttctc atcctgctga ctacataaat
      841 ggcgatacct tggtttggaa ttttacgaat ctgaacaact tggcaaataa tactccttat
      901 tttgatcagt ttttgggagg aatccaccta accccttccc tttctgttaa tattggtgat
      961 acactttggt ttgatttgag tacttcagtt gcaactggtg atgtaaatcc tgcaaacaac
     1021 catttgcatt ttggcattcc aattgttaat tcgtttgatc caaatattaa agaagtttca
     1081 ccaaaaggag ttggaattga aggacttatt cctatggaaa cagaagcact tacatacacc
     1141 attcactttc aaaacactgg aacagccgaa gcgataaata tatttgtatt agacacattg
     1201 acggcttcaa ttgatccgaa atctttgcaa atattagcga atagtcacaa catgactcca
     1261 acttggatta atgactctgt tgtaaagttt aatttcccta atattcattt agcagatagt
     1321 tcaagtaacg aacctgaaag tcatggtttt gtgcgattta aagtgaattt aaaagataat
     1381 ttacctattg ggacaatcat caaaaataaa gcagccattt attttgatac aaacgagcca
     1441 gttatcacta attttactac caacaaatta gcaaatgatt tatctgtttc aaaaattgaa
     1501 aaattaaatg atatttctgt tttcccaaat ccggcaaaag attatgtaat tatccgtttt
     1561 tttgagcaaa tcaacggtac aattgagcta tttgacttaa ctggtaagaa tgtttatact
     1621 gaagaagtaa agcaaatcag ccaaaaacaa attgatgtca agcagttaca aaatggcgtt
     1681 tatatctacc aaattaaagc caataatggg caaagaaaaa gtggcaaatt gattataaat
     1741 taaaattaaa aatttgtttt tattgtttta gaggttggca taaaatttgt taacctcttt
     1801 ttttgtgatt ataattgtgt ttatcctagt gtttttttga gaaaatacgt taaaaacact
     1861 ctgatttaat taatgacatc tcataagaaa cattaaattt gcaagaaaaa cacttttgaa
     1921 gagattaact gtatttagca ttcgctcttt tatcggtcca ttttttgtga cgtttgctat
     1981 tgctatgttc attctgatca tgcagttttt ctggaaatat atcgatgatt tgatgggaaa
     2041 aggaattgaa accatttaca ttcttcaatt gcttttttat gtttctgcta gtttaattcc
     2101 acttgcattg ccacttgcta tgttgctttc gtctattatg acactaggaa atttggccga
     2161 attcaacgag ttaacagctt taaaatcaag tggtttgtcg ctatacagaa ttttacgtcc
     2221 tttaacagcc attgttttaa ttttggcatt atctactttt ttgtttgcca actatgttat
     2281 tcctgttgct aactacaaat ggcgttcttt aatttacgat tttcaaaaca caaaaattgc
     2341 ttctatcatc gttccaggtg tatataccaa agaaattgac ggatatgcca tcaaggccaa
     2401 taaaaaaaat ccagatggct caatggaaga tattaccatt cacgataaaa cgaaacccaa
     2461 cgagtttaaa accatcaaag cagcaaaagg aaccttgtat aaatcagata atggtaatat
     2521 cttccattga gccatctgga ttttttt
//
LOCUS       DAOQVQ010000364         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_49371_length_2545_cov_2.109639, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000364 DAOQVQ010000000
VERSION     DAOQVQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49371_length_2545_cov_2.109639"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1861)
                     /gene="pckA"
                     /locus_tag="PKN22_09655"
     CDS             complement(266..1861)
                     /gene="pckA"
                     /locus_tag="PKN22_09655"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNS43011.1"
                     /translation="MEKLDLSKYGISGNFEVLHNPTYEELFQAEMDPANEGFEKGVLT
                     TTGAVSVDTGKFTGRSPKDKFIVLDDTTRDTMWWDGTINRPTTPEVFDHCKDLVTKQL
                     STAKTLYVVDTFCGTNEDTRMKVRFVMEVAWQAHFVTNMFIRPSHYELANYGEPDFVC
                     LNGSKTVNPNWKEQGLNSEVFTLFDLTRKMQVIGGTWYGGEMKKGIFALMNYYLPLKG
                     IASMHCSANVGAEGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDNHGVFNYEGGC
                     YAKVIDLEKDKEPDIWRAIRRDALLENVTVNADGTPDYTAAASKTENTRVSYPIYHIN
                     KIVSPSRAGHAKKIIYLSADAFGVLPPVSILDEKSAQYHFLSGFTSKLAGTERGITEP
                     VPSFSPAFGEAFLTLHPTMYAKTLIGKAHEHGAKVYLVNTGWNGTGKRISLKNTRAII
                     DAIIDGSIENAPTIHVPILNLVAPTELNNVDTEILDPRNTYADVAEWETKAKSLAAKY
                     IKNFEQYCDNDDAKALIPSGPQL"
     gene            2349..>2545
                     /locus_tag="PKN22_09660"
     CDS             2349..>2545
                     /locus_tag="PKN22_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HNS43012.1"
                     /translation="MKTIKLKKGLNIEISGKASLHVASMSQPETVAIVPDDFKGITPK
                     VAVKEGDTVKAGSVLFFDKTHP"
BASE COUNT          828 a          476 c          458 g          783 t
ORIGIN      
        1 tgataccaac gaaataaaat gtatttgtaa tactgtaatg gtctgcttcg atgcaaaaac
       61 caagactaca cgtcaaattt cggatgaatg gcgcaaggcg atggctgatt ttgaaatgaa
      121 tcctgaattg aataaaatta gtagccacta actctgtttt agtattatct ctgtaaaaaa
      181 aacgagctgt ccgaaattat cgaacagctc gttctgttta tttcaaacca agtccaaaat
      241 cttatgtctt ggttcttttg tcttcttaca attgtggtcc cgaaggaatc aatgcttttg
      301 catcgtcgtt gtcgcaatac tgttcgaagt tcttgatgta tttagcagca agcgatttag
      361 ctttggtttc ccattcagct acatcagcat aagtgttacg tgggtcaaga atttcagtat
      421 ccacattgtt caattcagtt ggagctacca ggttcagaat tggaacgtga attgttggag
      481 cattttcgat tgaaccgtcg atgatagcgt cgatgatagc acgtgtgttt ttcaacgaaa
      541 tacgttttcc tgttccgttc catccggtgt taaccagata aacttttgca ccgtgttcgt
      601 gagctttacc aatcaaagtt ttagcataca ttgttgggtg cagtgttaag aatgcttcac
      661 caaatgcagg cgagaaagaa ggaaccggtt cagtgatacc gcgttctgtt ccggctaatt
      721 ttgaagtaaa gcctgacagg aagtggtact gagctgattt ttcgtcaaga attgataccg
      781 gaggcaatac accgaatgcg tcagccgaca ggtaaataat tttcttagcg tgacctgcac
      841 gtgaaggaga aacgattttg ttgatatgat aaattggata agaaacgcgt gtattttctg
      901 ttttagaagc agcagctgta taatcaggag tgccgtcagc atttacagtt acgttttcca
      961 acaaagcatc gcgacgaatt gcacgccaaa tatcaggttc tttgtctttt tccaaatcaa
     1021 taactttagc atagcaacca ccttcgtagt tgaatacacc gtggttatcc caaccatgtt
     1081 cgtcgtcacc aatcaaataa cgttttgggt cagcagaaag ggttgtttta cctgttccag
     1141 aaagaccgaa gaaaatagca acatcgcctt ctgcacctac gttagcagag cagtgcattg
     1201 aagcaatacc tttcaatggt aaatagtagt tcatcaaagc aaaaatacct tttttcattt
     1261 caccaccata ccaagtacca ccaataactt gcattttgcg agtaagatca aacaaagtaa
     1321 acacttctga atttaaacct tgttctttcc agtttggatt aactgttttt gaaccgttca
     1381 aacatacgaa atcaggttca ccgtagttag ccaattcgta gtgtgaagga cgaataaaca
     1441 tgttagttac gaagtgagct tgccatgcaa cctccataac gaaacgcact ttcatacgag
     1501 tgtcttcgtt agttccgcag aatgtatcta ctacataaag agtttttgca gtagaaagtt
     1561 gtttagttac aagatcttta caatgatcaa atacttcagg agtagttggg cggttaatag
     1621 tgccatccca ccacatagta tcgcgtgtag tatcgtccaa tacgataaat ttatctttag
     1681 gagaacgacc tgtaaattta ccagtatcaa cagatacagc acctgtagtg gtcaacactc
     1741 ccttttcgaa accttcgtta gcaggatcca tttcggcttg aaacaactct tcgtaagttg
     1801 ggttgtgcaa cacttcgaaa ttaccggaaa ttccatactt gcttaaatcc aatttttcca
     1861 taatgttaat tgttttataa tgattaatac tctacttgca ttttatactt taagacgtta
     1921 caaagataca acaaaaatca aacccgataa agcaattaaa gaaatttttc tttaattaaa
     1981 tcaaaataaa ataaagtatt cattataaaa taattaacaa tgctcatttc ggctctattg
     2041 tgcaaaaaaa gaatttttag tgcttaattt tgacattttt attaaggatt tacaccccgc
     2101 ttaatcacat ttttttctta aaaaaagcac tccttttaaa aaatcagaaa aacagattag
     2161 aaaagaagat gcaacaactt ataactcaaa aaaagtgacg taaactgggt taattgaact
     2221 agtaaaaaat aataaaatat taaacaaaaa aagggttgaa tcttttaaaa ttatattgta
     2281 cctttgcggc gcaaaaaaat aaaacctatt gttttgcact gattttgaat ttatttcaat
     2341 tcatttatat gaaaacaatc aaactgaaaa aaggattaaa cattgaaatt tcgggcaagg
     2401 catctttgca tgttgcttcg atgagtcaac ctgaaacggt ggccatcgtc cctgacgatt
     2461 tcaaaggaat cactcctaaa gtagccgtaa aagaaggaga taccgtgaaa gctggtagtg
     2521 tccttttctt tgacaaaaca catcc
//
LOCUS       DAOQVQ010000365         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_49521_length_2540_cov_2.917907, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000365 DAOQVQ010000000
VERSION     DAOQVQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49521_length_2540_cov_2.917907"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKN22_09665"
     CDS             <1..288
                     /locus_tag="PKN22_09665"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HNS43013.1"
                     /translation="ASTSDEYFQSRPWESKIGAWASHQSEKLASRNELEQRIEKLAAQ
                     YPTDVPRPPHWGGYAITPSKIEFWQGRPSRLHDRFVFEKNGSQWTISRLNP"
     gene            313..1554
                     /locus_tag="PKN22_09670"
     CDS             313..1554
                     /locus_tag="PKN22_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNS43014.1"
                     /translation="MKFAIHPEVVEFSNGLRLVYLYKDTFVGHFGTTILAGSRYEQEN
                     EQGLAHFIEHCIFKGTTNRKAYHILSRLDAVGGELNAYTTKEEIAIYASFTNQHLKRA
                     IELIADITFHSTFPEKEIEKEKEVVLDELNSYLDAPSDRIFDDFEAKLFKNHSLGYNI
                     LGTEESVRSFTKEHLKNYTNRFFSLKNMVISYVGNESLAQLKKWVEKYFFNENDQVVS
                     AKLETFQPQPPFSITEQHGNVQTHAIIGGFAPNYSDNDRRGMILLTNILGGPALNSRL
                     TLSIREKYGYSYNIEANYTPYVDTGFWSIYLGTDEKYLQKSINLVRKELKNSCDTKMG
                     TLQLNQAKEQLKGQIALSLEGNAGLMLGLGKSLLLFNQIDTIPDIYKEIDNLTASDIL
                     HVANTYFGTSNQNELIYALKK"
     gene            complement(1556..2356)
                     /locus_tag="PKN22_09675"
     CDS             complement(1556..2356)
                     /locus_tag="PKN22_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNS43015.1"
                     /translation="MKYIVLLSILMVLTLSFSQQDSIPEHETFQIHSSILKEDRTINV
                     WLPKAHSNTQKLPVLYMLDGGIKEDFPHLANTLSKLMEEKSIPPMMLVGIENIKRRKD
                     LTGYTTSKYDKTIADEVGGAENFRKFIVTELIPAIQKKYATTNSSSIIGESLAGLFIL
                     ETFFLQPEVFDHYIAFDPSLWWNNYHYPKNLDTYLKDLPYPGKSLWFGASNTKSITQP
                     NRKLAKTLKKKNCANLRWYYSDEVQEKHNTIFRAAKERALKWTFGNEQ"
BASE COUNT          835 a          444 c          456 g          805 t
ORIGIN      
        1 gcatctacaa gtgatgaata ctttcaatca cgtccgtggg aaagtaaaat tggggcatgg
       61 gctagtcatc aatcagaaaa actagcttct cgcaatgaat tagaacaacg catagaaaaa
      121 ttagcagcgc aatatccaac agatgttcct cgtccaccac attggggagg atatgcaatc
      181 acaccttcta aaattgaatt ttggcaaggt cgtccatctc gtttgcacga tcgttttgtg
      241 tttgaaaaaa atgggagtca atggacgatt tctcgcttaa atccataaat taaaaaaaga
      301 gaacttttct acatgaaatt tgctattcat cccgaagttg ttgaattctc aaatggttta
      361 agactggttt atttgtacaa agacacattt gtagggcatt ttggcaccac aattttggct
      421 ggttcacgct acgagcaaga aaacgaacag ggattggcac atttcattga acactgtatt
      481 tttaaaggaa caactaatcg taaggcatat cacattcttt ccagattaga tgctgttggt
      541 ggtgagttaa acgcctatac caccaaagaa gaaattgcca tttatgcatc gtttaccaat
      601 caacacttaa aacgagctat tgaattaatt gccgacatta cgtttcattc tacttttcca
      661 gaaaaggaaa ttgagaaaga aaaagaagtg gttttggacg agttgaattc gtatttggat
      721 gctccaagcg atcgtatttt tgatgatttt gaagcaaaat tgtttaaaaa ccattcgttg
      781 ggatacaata ttttaggcac agaagaatcg gttcgtagtt ttacaaaaga acatttaaaa
      841 aattacacca atcgtttttt ctcgttaaaa aatatggtga tttcttatgt tggcaatgaa
      901 agtttggcgc aactaaaaaa atgggttgaa aagtattttt tcaatgaaaa cgaccaagtt
      961 gtatctgcca aattggaaac atttcaacct caacctcctt tttcaataac ggagcaacat
     1021 ggaaatgtgc aaacccatgc aattattggt ggttttgcgc ccaattacag cgataacgat
     1081 agaagaggga tgattttgtt gaccaatatt ttgggtggac cagcactcaa ttcccgttta
     1141 actttgtcca ttcgcgaaaa atatggatat tcatacaaca tcgaagccaa ttacacacct
     1201 tatgttgaca ccggattttg gagcatttat ttaggcaccg acgaaaaata tttgcaaaaa
     1261 tccatcaatt tggtgcgcaa agaactcaaa aatagttgtg atacaaaaat gggaaccttg
     1321 caattaaacc aagcaaaaga gcaactaaaa ggacaaatag ccttgtcatt ggaaggaaat
     1381 gctggattga tgttgggact tggaaaaagc ttgttgttgt tcaatcaaat tgacaccatt
     1441 ccagatattt acaaagaaat agacaattta acagcatctg atattctgca tgttgccaac
     1501 acctattttg gaacgtcgaa tcaaaatgaa ttgatttacg cattgaaaaa ataagttatt
     1561 gctcatttcc aaacgtccat ttcaacgctc gttcttttgc agctctaaaa atggtattgt
     1621 gtttttcttg cacttcatcc gaataatacc aacgtaaatt agcgcaattt ttctttttca
     1681 acgtttttgc caatttgcga ttgggttgag taattgattt tgtgtttgat gccccaaacc
     1741 aaagcgattt tccaggatag ggcaaatctt tcaaataagt atccagattt ttgggataat
     1801 ggtaattgtt ccaccacaat gatgggtcga aagctatgta atgatcaaat acttcgggtt
     1861 gaagaaaaaa tgtctctaga ataaacaaac ctgccaatga ttcaccaata atggatgaag
     1921 aattggttgt tgcatatttt ttttgaatgg ctggaattaa ttctgttaca ataaattttc
     1981 taaagttttc ggcaccgccc acctcatctg caatggtttt atcatatttg gaggtggtat
     2041 aacctgttaa atcttttctt cttttaatgt tttcaatgcc taccaacatc attggaggga
     2101 tggatttttc ttccattaat ttggaaagtg tattggcaag atggggaaaa tcttctttaa
     2161 ttccgccatc caacatatac aaaacaggca atttttgagt gttagaatgt gcttttggca
     2221 accaaacatt gatggttctg tcctctttta aaattgatga atgaatttga aacgtttcat
     2281 gctcaggaat gctatcttgc tgagaaaaac taagcgttaa taccattaaa atcgaaagta
     2341 aaactatgta tttcataaat catacaattt gaatgaatta taaaaataca caattttatt
     2401 cagcaaaatc aaatccaaat tgttcggcca ttttttgctt gagaatattg ctcacttctg
     2461 ccatatccac ttctctaccc aattcctttt tcattgaagt aacgccttta tcttgaatgc
     2521 cacacggtac aatgtgcgaa
//
LOCUS       DAOQVQ010000366         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_49648_length_2536_cov_3.436114, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000366 DAOQVQ010000000
VERSION     DAOQVQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49648_length_2536_cov_3.436114"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PKN22_09680"
     CDS             complement(<1..1331)
                     /locus_tag="PKN22_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNS43016.1"
                     /translation="MLRNFLVSFFIGLSAHTFSQICHNSYIGIVLDEHDLSPVTSAKI
                     VFKDLNKSYSTNSSGNFKIDSLCAGEYQVLFYHHIGCEPVKKKITLPMEGVDTIYLEY
                     HYDNEVTELNELEVIKVQQPVKPFLPTVHLDSKEAKLTTGDNLGSILGNLPGVTTMSS
                     GATIQKPVMSGMYGNRIATIVEGTKLEGQQWGNEHAVEINPYLFDKITAIKGATSLMY
                     GSDAIGGVLITDLSPVAAKPMSGKANLGYASNGNQLFGNLKLGGRFASNKWSWIAQGA
                     YKKGGTLSTPTYNLANTAMNELNSGIGFSYTGQKWHSQVVYSYNQSTNGIFQGAHAHD
                     ADELYEAMALSQPAINQKFTYNIDAPYLQTKHHLASWNNHLVLNDKNSLDIAYSFQYN
                     QRAEIHDHHDEEHEEEEDHDHDHDHDHDHDHEEAEGLGFNLLTNQFSVKYN"
     gene            complement(1428..1703)
                     /gene="yajC"
                     /locus_tag="PKN22_09685"
     CDS             complement(1428..1703)
                     /gene="yajC"
                     /locus_tag="PKN22_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNS43017.1"
                     /translation="MMGQQLIMILLMVAVFYFFMIRPQMKKQKELKKFRESLAQGDKV
                     VTIGGIHGKILEVTDTTVLLSCEGAKLRFEKSAISQTVADQLTSTEG"
     gene            complement(1723..2106)
                     /locus_tag="PKN22_09690"
     CDS             complement(1723..2106)
                     /locus_tag="PKN22_09690"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNS43018.1"
                     /translation="MRTLFFALSLVLLSACGNNEVKIGNRTTVEYKNVYKAGKVSKGE
                     IIKAKIELKNTGDYPLVVGDVEVACSCTLASKPEKPIKPGETGVVEASVDTDKVGFGR
                     FSRDIRIIANTTPSVLVVSIQGEVN"
     gene            complement(2113..>2536)
                     /locus_tag="PKN22_09695"
     CDS             complement(2113..>2536)
                     /locus_tag="PKN22_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HNS43019.1"
                     /translation="IDFACSMVLKTIKSLESETSELQILDLYKDEEDEKAFTTGLFKN
                     TILMNKANEALIDELTQNWELDRIAKMDIILMKMAITEFQIFNTIPTKVTLNEYIEIS
                     KFYSTPKSNNFINGVLDKAIDRLQAEGKIKKVGRGLIN"
BASE COUNT          759 a          493 c          401 g          883 t
ORIGIN      
        1 tggttgtatt ttacagaaaa ttggttggtc aacaagttaa aacccaaacc ttctgcttct
       61 tcatgatcgt ggtcatggtc atggtcatgg tcgtggtcgt ggtcttcctc ctcttcgtgt
      121 tcttcatcat ggtggtcgtg aatctctgct cgttgattgt attgaaaaga ataggcaata
      181 tccaaactat ttttatcatt caaaacaagg tgattattcc acgaagctag atggtgcttg
      241 gtttgcaaat agggagcgtc tatgttgtag gtgaattttt gattaattgc cggctgactc
      301 aatgccatgg cttcgtataa ctcatcggca tcgtgcgcgt gtgccccctg aaaaattccg
      361 ttggtagatt ggttgtatga ataaaccact tgcgagtgcc atttttgacc cgtataacta
      421 aaaccaattc ccgaattcaa ctcattcata gcggtgttgg ctaaattata ggtaggtgtt
      481 gacaatgttc cacctttttt ataagcacct tgagctatcc aactccattt attagatgca
      541 aaacgccctc ccaattttaa gttaccaaac aattgattgc cattacttgc ataacccaaa
      601 ttggcttttc cagacattgg ttttgctgcc acaggagata aatctgtaat taacacgcca
      661 ccaattgcat cagaaccata cattaatgat gttgcacctt taatggctgt tattttatca
      721 aataaatacg gattaatttc aacggcatgt tcatttcccc attgctgacc ttctaatttg
      781 gtgccttcaa caatggttgc aatacgattg ccatacattc cactcatcac tggtttttga
      841 atggtagccc cagaagacat ggttgtaact ccaggtaaat ttcctaaaat tgaccctaaa
      901 ttatcaccag ttgtaagttt tgcttctttt gaatcaagat gaactgttgg taaaaatggt
      961 tttacaggtt gttgcacttt tatcacctcc aattcattta actccgtcac ttcattgtcg
     1021 tagtggtatt ctaaataaat ggtatcaact ccctccattg gtaaagttat tttcttttta
     1081 accggctcac agccaatgtg gtggtaaaac aaaacttggt attcaccagc acacagacta
     1141 tcaattttaa aattaccaga agaattggta gaatagcttt tattaagatc cttaaacaca
     1201 attttagcac ttgtaactgg agataaatcg tgttcatcta aaactattcc aatataagaa
     1261 ttgtgacaaa tctgagaaaa agtgtgagca gaaagcccta taaaaaaaga aacaaggaaa
     1321 ttacgtaaca taaatatagg attgatttgt gtgtaaaaaa tataaaaacg acaaaaaccg
     1381 atgatgtact caccggtttt aaatttcttt tgaaaacgta aaataaatta cccttctgtt
     1441 gacgttaatt gatcagcaac agtttgagaa atagctgatt tttcaaaacg caattttgct
     1501 ccttcacagc tcaataaaac agttgtatca gtaacttcta aaattttacc gtggatacca
     1561 ccaattgtaa ctactttatc cccttgcgct aaactttctc tgaatttttt taattctttt
     1621 tgttttttca tttgaggtcg aatcatgaaa aaataaaaca ctgcgaccat caacaaaatc
     1681 atgattaact gttgtcccat catacttttt tttgtttgtt tattaattta cttcaccttg
     1741 tatagaaacc actaacacag acggagtagt gttggcaatg atgcgaatat ctctactaaa
     1801 tctaccaaaa cctactttat ctgtatcaac agacgcttct actacaccag tttctcccgg
     1861 tttgattggt ttttcgggct tacttgcaag tgtgcaacta caagctactt ccacatcacc
     1921 aaccaccaac ggatagtcac cggtattttt taattcaatt tttgctttaa taatttcacc
     1981 tttagaaact ttacctgcct tgtaaacatt tttatattca acagtggttc tgtttccaat
     2041 tttcacttcg ttgtttccgc atgcagaaag taaaacaagt gataaagcaa aaaataatgt
     2101 tctcattttt aattaattta taagtccgcg accaactttc ttaatttttc cttcggcttg
     2161 caatctatca atagctttat ctaaaactcc attgataaag ttattgcttt ttggcgtgga
     2221 gtaaaattta gaaatttcaa tgtattcgtt caacgttact ttggttggaa tggtattaaa
     2281 aatttggaat tctgtaatgg ccattttcat taaaatgatg tccatttttg caattctatc
     2341 caactcccag ttttgtgtca attcgtcaat taacgcttca ttggctttgt tcatcaaaat
     2401 cgtattttta aacaaacctg ttgtaaacgc tttttcgtct tcttcgtctt tgtacaagtc
     2461 taaaatttgc aattcgcttg tttcagattc cagactttta attgttttca acaccatgga
     2521 gcaagcaaaa tcgata
//
LOCUS       DAOQVQ010000367         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_49819_length_2531_cov_3.018982, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000367 DAOQVQ010000000
VERSION     DAOQVQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49819_length_2531_cov_3.018982"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKN22_09700"
     CDS             <1..670
                     /locus_tag="PKN22_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43020.1"
                     /translation="PPLKPVVNKLSFQQYDFMNIGKGGVYARADTCIYVIYNENLPYV
                     TINKKDSTVVLSKDVWGEYTHAYELKDKRKCLIPANSNETPWILYYDGIFSGSYIELT
                     PIYDSSLQLLLNIPEALENTFLRPYFNDPPHSIYKYFSMIDNYGLLLVFIVVLLFFRR
                     KLSTIEIALIAALVTFSFVLALLIGWTTPVIGAIVRYKIPIQLAMMLITIIVFDFKKV
                     MKNG"
     gene            663..1424
                     /locus_tag="PKN22_09705"
     CDS             663..1424
                     /locus_tag="PKN22_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS43021.1"
                     /translation="MDKYVFITGATSGIGKACAQEFAKNGFHLIITGRRKERLVELQQ
                     ELTQFGVDVQCLHFDVRDLDAVKTAILSLSEATKQKIAILINNAGLAVGREPIEMGDI
                     DDWNRMIDTNIKGLLYVSKEVIPILKQNKAGHIFNIGSIAGKEVYPMGNVYCASKHAV
                     DALSKAMRIDLVNEGIKVTNIAPGAVETEFSLVRFKGDQAKADATYNGFEPLIAQDIA
                     ETIYFVATRPQHVTINDLVIMPTAQASAVVFHKKQ"
     gene            complement(1473..2129)
                     /gene="fsa"
                     /locus_tag="PKN22_09710"
     CDS             complement(1473..2129)
                     /gene="fsa"
                     /locus_tag="PKN22_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNS43022.1"
                     /translation="MKFFIDTANLNEIKEAYELGILDGVTTNPSLMAKEGITGEQNIL
                     NHYVAICNIVDGPVSAEVIATDFEGMIKEGEALAKLHKNIVVKVPMIPEGIKAIKYFS
                     SKGIKTNCTLIFSAGQALLAAKAGATYVSPFLGRLDDISFDGLELIAQIRTIFDNYLF
                     ETEILSASVRHPMHIIRCAEIGSDVATCPLNAIKALANHPLTDKGLQQFLADYAKGNK
                     "
     gene            2207..2440
                     /locus_tag="PKN22_09715"
     CDS             2207..2440
                     /locus_tag="PKN22_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNS43023.1"
                     /translation="MENTEIWLDSLITKTPQEGRELAIKMARKTIGAIQSDPEVKKRL
                     RPQYAEDAGLLVASAEVVALEFQTVAAANNYWR"
BASE COUNT          823 a          434 c          458 g          816 t
ORIGIN      
        1 cccaccgtta aaaccagtgg ttaataaatt atcgtttcaa caatacgatt ttatgaacat
       61 tggcaaaggt ggcgtttatg cacgagcaga tacttgtatt tatgttattt acaatgaaaa
      121 tttgccgtat gttacaatca acaaaaaaga ttctacggtt gtgctttcta aagatgtttg
      181 gggagaatat acccatgcct acgagttaaa agataaacgc aaatgtttga ttcctgccaa
      241 tagtaatgaa acgccttgga ttttgtatta cgatggaatt tttagcggaa gttacataga
      301 gttaacgcca atatatgact ctagtttaca gcttttacta aatattccag aagcactaga
      361 aaatacattt ttacgtccct atttcaacga tccaccacat tctatttaca aatatttttc
      421 tatgattgat aattatggct tgttgctcgt ttttattgtg gtattgcttt tttttagacg
      481 aaaattatcc accattgaaa tagcactaat tgctgcgttg gtaacttttt cttttgtatt
      541 agctctatta attggttgga ccacacctgt tataggtgca attgttcgtt acaaaatccc
      601 tattcaattg gctatgatgc taataacaat cattgtgttt gacttcaaaa aagtaatgaa
      661 aaatggataa atatgttttt ataaccggtg ccacttctgg aattggcaaa gcatgtgcac
      721 aagaatttgc aaaaaatggt tttcatttga ttataactgg ccgcagaaaa gaacgtttag
      781 ttgaattgca acaagagtta acacaatttg gtgttgatgt gcaatgcctt cattttgatg
      841 ttcgggattt agacgccgta aaaacagcca tactatcact ttcagaggca accaaacaaa
      901 aaattgctat tttaattaac aatgctggtt tggcagtagg aagagaacca attgaaatgg
      961 gcgatattga cgattggaac cgcatgattg acaccaacat caaagggttg ttgtatgttt
     1021 caaaagaagt tattccgatt ttaaaacaaa acaaagccgg tcatattttt aacattggaa
     1081 gcattgctgg caaagaagtt tacccaatgg ggaacgtata ttgtgcgtca aaacatgctg
     1141 ttgatgcact ttcaaaagcc atgcgtattg atttggtaaa cgaaggaatt aaagttacca
     1201 atattgcccc aggtgcagta gaaacagaat tttctttggt gcgttttaaa ggcgatcaag
     1261 caaaggcaga tgctacctac aatgggtttg aaccgttaat tgcacaagat attgcggaaa
     1321 ctatttattt tgttgcaaca cgcccacaac atgttaccat caacgatttg gtgattatgc
     1381 caacagctca ggcgagtgct gtggtgtttc ataaaaaaca atagaggctg aaattactcc
     1441 aacctctatc ctgttttttg aatcgttatt tcttatttat ttccttttgc gtaatctgct
     1501 aagaattgtt gcaatccttt atcagtcaac gggtgatttg ccaatgcttt gatagcattt
     1561 aaaggacaag ttgctacatc cgacccaatt tctgcgcaac gaataatgtg cattgggtga
     1621 cgaacagaag ctgaaagtat ctctgtttca aacaaataat tatcaaaaat agtgcggatt
     1681 tgagcaatca actccaaccc atcaaaagaa atatcgtcta atcttcctaa aaacggagac
     1741 acataagttg cacctgcttt tgccgctaat aaagcttgac cagcagagaa aatcaacgta
     1801 caattagttt taattccttt tgaagaaaaa tatttaattg ctttgatacc ttctggaatc
     1861 attggtactt ttaccacaat atttttgtgc aattttgcca atgcttcgcc ctctttaatc
     1921 atcccttcga aatcggtagc tataacttct gcagaaactg gaccatccac aatattacaa
     1981 atggcaacgt agtgatttaa aatattttgt tcgcccgtaa tgccttcttt agccatcaat
     2041 gacgggttgg ttgtaactcc atccaaaata cctaattcat aggcttcttt aatttcattt
     2101 agatttgctg tgtctataaa aaacttcata tttctttttt ttgcaaaggt aagaaatgta
     2161 ttcgtattta aaatttgtaa atttgaagag tatcaaaatt taagttatgg aaaatacaga
     2221 aatatggttg gatagtttaa ttactaaaac accacaagaa ggaagagaat tggcaattaa
     2281 aatggcaaga aaaacaatag gtgccatcca atctgatcct gaagtaaaga aaagattgcg
     2341 cccgcaatat gccgaagacg ctggattatt ggttgcatct gctgaagtgg tagcattgga
     2401 atttcaaaca gttgctgctg ccaataatta ttggagataa gcaatttccg taaaatgtaa
     2461 aaaggtcact caattgagca cgaatggtcg gaagaaattt cttccgactt ttttgtattt
     2521 attaaccttt a
//
LOCUS       DAOQVQ010000368         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_50057_length_2523_cov_5.132091, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000368 DAOQVQ010000000
VERSION     DAOQVQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50057_length_2523_cov_5.132091"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2523)
                     /gene="porU"
                     /locus_tag="PKN22_09720"
     CDS             complement(<1..>2523)
                     /gene="porU"
                     /locus_tag="PKN22_09720"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNS43024.1"
                     /translation="NPVVGAASTATISMASNSTSPSYGSLTYTVNGTQIANFAVPSNG
                     QGYYGRAEGQYNFNNSNSTIQLAINFQRNNPSVIAYLDKIEINSRRQLVFWDNQFSFR
                     DLASVGAGNIADFSIQNFPTNGFVWDVTDRKHPKLVQGTLSGSTYNFQAATDSLNEYV
                     ASNGSNFYEPVFIERVNNQNLHALAQVDYLIVTPPDFMSQANRLADLHRNNGLTVHVV
                     NINTIYNEFSSGGKDPGAIRQFAKMFYDRSTSAADQIKYLCLFGDGTYDPKNRISSNN
                     NYVPTYQILGNNYSESPIMNIPADDFYGLLDNSDAMDAVSVVDIGVGRILVSSTQIAQ
                     EQVNKIEHYMKGGSNFYTSNNVNCVDGVSTTSFGDWRTKLVNVGDLEDYFVLTDLEPI
                     YNNTKLNHPEINVNKLYLDAYKLESTVAGNRFPTLNEALLNSFYSGSLIINYVGHGSV
                     YQLSESRVLTKAIIQDLKNSDRLPLFVSATCEFTRFDDPSFVSSGEWMVLNPIGGAIA
                     MMTTTRTVSYSINSNTISAFFGTVFDRKADGSPQTFGDIILSTKTSQTAASLDKTAFL
                     LIGDPALKIALPQQKIVIDSINGLSPNLVMDTLQALSKVKVKAHLEDQNGNILSSFNG
                     VATPSLYDKPKQLKTLGQKPGPSNIGYANEHPFELQKNIIYRGQSTVKNGYFSFEFIV
                     PKDIDYSYGNGKFSLYADNGSKDAVGAEQRVIVGGVNPNGLNDDVGPEIDIYLNNENF
                     VNGGITDETPFLIAKLKDENGINTVGNGIGHDITAVIDAKTSSPIVLNEYFKNDLDSY
                     QKGELRYQLSKLEPGRHTLTLKAWDVNNNSSETTIDFIVQEK"
BASE COUNT          759 a          491 c          448 g          825 t
ORIGIN      
        1 ttttttcttg cactataaaa tcaatggtag tttctgaaga attgttgttt acatcccacg
       61 cttttagtgt caatgtatgc ctaccaggtt ccaacttact taattggtaa cgcaattctc
      121 ctttttggta ggaatccaaa tcgtttttaa aatattcgtt caaaacaatt ggtgaactgg
      181 tttttgcatc aatcacggca gtaatatcat gcccaattcc gttcccaacc gtattaattc
      241 cgttttcatc ttttaattta gcaattaaga acggagtttc gtctgttatt cctccgttta
      301 caaaattttc gttgtttaaa taaatgtcta tttctggacc cacatcatcg tttaacccgt
      361 ttggattaac acctccaaca ataacacgtt gctctgctcc aacagcatct tttgaaccat
      421 tgtcggcata aaggctaaat tttccatttc cataactgta atcaatgtct tttggaacga
      481 tgaattcaaa cgagaaataa ccatttttta cagttgattg ccctctgtaa ataatatttt
      541 tttgcaattc aaacggatgt tcgttggcat aaccaatgtt gctcggacct ggtttttgcc
      601 ccaaagtttt caattgtttg ggcttgtcgt aaagtgatgg cgttgcaact ccattaaacg
      661 agctaagaat gttaccattt tggtcttcca agtgtgcttt cactttcact ttgcttaatg
      721 cttgaagggt gtccattacc aagttaggac tcaatccatt gatgctatca ataacaattt
      781 tttgttgagg taaagcaatt tttaacgcag gatcgccaat caataaaaag gctgttttgt
      841 ctaaacttgc tgctgtttgt gaagtttttg tacttaaaat gatatctcca aaagtttgag
      901 gtgaaccatc cgccttgcgg tcaaatacag ttccaaaaaa tgcagaaata gtgttggaat
      961 taatgctgta actaaccgtt cttgtggtgg tcatcatggc aattgcgcct ccaattggat
     1021 tcaaaaccat ccattcaccc gatgaaacaa atgaagggtc gtcaaaacga gtaaattcac
     1081 aggttgccga cacaaataat ggcaaacgat ccgaattttt taaatcttga ataatggctt
     1141 ttgttaatac gcgtgattcg gaaagttggt aaacactacc atgccccaca taattgataa
     1201 ttaagctgcc agaataaaaa ctgttcaata aagcttcgtt cagcgttggg aaacgatttc
     1261 cagctacggt tgattctaat ttataggcat ccaaatacaa tttattgaca ttgatttctg
     1321 gatgattgag tttggtattg ttataaattg gttctaagtc ggttaacaca aaataatctt
     1381 ctaggtcacc aacatttacc aattttgtgc gccaatctcc aaaacttgta gtagaaaccc
     1441 catctacaca attaacatta ttgctggtat aaaaattaga accacctttc atgtagtgtt
     1501 caattttatt aacttgttcc tgagcaattt gcgtagaaga aaccaaaatt cgtcccacac
     1561 caatatccac aacagagaca gcatccatgg catccgaatt gtctaaaaga ccataaaaat
     1621 catcggctgg aatgttcatt atgggcgatt ctgaataatt attccccaaa atttgatagg
     1681 tgggtacata attgttgttg ctcgaaattc tgtttttagg atcgtaggtt ccatcaccaa
     1741 acaaacacaa gtacttgatt tggtctgctg ctgatgtgga acggtcataa aacatttttg
     1801 caaattgacg aatagctcca ggatctttac caccgctact aaactcgttg taaatggtgt
     1861 tgatattcac cacatgaact gttaaaccat tattgcggtg taggtcggcc aaacgattgg
     1921 cttgtgacat aaaatcgggt ggagtaacta ttaaataatc aacttgtgca agcgcatgca
     1981 aattttgatt gttcacacgc tcaataaata cgggttcata aaaattggaa ccattgctag
     2041 ccacatattc attcaagcta tcggttgccg cttggaaatt ataggttgac ccactcaatg
     2101 ttccttgcac taatttagga tgttttctat ccgttacatc ccaaacaaaa ccatttgtag
     2161 ggaagttttg aatactaaaa tcggcaatat ttcctgctcc aacagatgct aaatctctaa
     2221 aagaaaactg attatcccaa aacaccaatt ggcgtcgtga attgatttct atcttatcca
     2281 aatatgcaat tacacttgga ttgttgcgtt gaaaattgat tgctaattgg atggtgctgt
     2341 tagaattgtt gaaattatac tgaccttcag ctcttccata atacccttgt ccattgcttg
     2401 ggactgcaaa attggcaatt tgcgtaccgt ttacagtgta tgttaaacta ccataagaag
     2461 gagaggtact gttggacgcc attgaaatgg tggcagttga agctgctcct acaacaggat
     2521 ttg
//
LOCUS       DAOQVQ010000369         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_50195_length_2518_cov_4.107998, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000369 DAOQVQ010000000
VERSION     DAOQVQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50195_length_2518_cov_4.107998"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKN22_09725"
     CDS             <1..689
                     /locus_tag="PKN22_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS43025.1"
                     /translation="SLFLLIFHPIQWICYKFFGYKAHKNSVDVLNFFLTYSTLVLFNV
                     PILKNKIQIPKGRPIIFVSNHQSTFDIPGLIYFFRKFHGKFISKIELAKSRIPSIAFN
                     LKYGGAANIDRKDAEQSKREIAKLAEKMNKLKWSVFIFPEGTRTKDGLMKKFKTGGIE
                     TLASICPEALIVPIAIKGSADMVKKGMFPLMPFHKLTWEVLSPIEIKGRNISEVVQEA
                     ENSIRNVVEG"
     gene            788..2431
                     /locus_tag="PKN22_09730"
     CDS             788..2431
                     /locus_tag="PKN22_09730"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNS43026.1"
                     /translation="MAVSKKELEFNINDDDLRLKISNLERTLAKIYEGGGKKRIEKLH
                     EQGKLTARERIDLLLDPDTERIEIGALAGYEMYPEHGGCPSGGVVIVIGYVKGKQCIV
                     VANDATVKAGAWFPITGKKNLRAQEIAMENRLPIIYLVDSAGVYLPMQDEIFPDKEHF
                     GRIFRNNAIMSSMGIVQIAAVMGSCVAGGAYLPIMSDESLIVNGSGTIFLAGSYLVKA
                     AIGENIDNETLGGATTHTSISGVCDYKAENDQECIATIRDLVDKIGQSENAGFDRKEA
                     VAPKHEPKSVYGIIPTDRSKPYNMKEVLERIVDNSEYTEYKADYGKTIICAYARIDGW
                     SVGIVANQREVIRSAKGEMQLGGVIYSDSADKAARFIMNCNQKNIPLVFLVDVTGFMV
                     GSKSEHGGIIKDGAKMVNAMANSVVPKFTIVVGNSYGAGNYAMCGKAYDPRLIVAWPT
                     AEIAVMGPEQLSGVMEMIQRQATERSGIPYDEQQGAMIRNHLMGEVEKQSNAWYASSQ
                     LWDDGVIDPRETRNYLGFCLAVVNNKPIQSSNTYGVFRM"
BASE COUNT          798 a          440 c          551 g          729 t
ORIGIN      
        1 tttcattgtt tttgttgatt tttcatccta ttcagtggat ttgttataag ttttttggct
       61 acaaagcaca taaaaatagt gtggacgtgc ttaatttctt tttaacgtat tcaactttgg
      121 tattgtttaa cgtgccgatt ttgaaaaata aaattcaaat ccccaaaggt cgaccaatta
      181 tttttgtttc caatcatcaa agtacgtttg acattcccgg gttgatttat ttttttagaa
      241 aatttcatgg taagttcatt tccaaaatag aattggctaa atctagaatt ccgagcattg
      301 ctttcaactt aaaatatggt ggtgctgcaa atattgaccg caaagatgca gaacaatcca
      361 aacgtgaaat tgcaaaattg gccgagaaaa tgaacaaatt gaaatggtcg gtgtttattt
      421 ttccagaagg tacacgcacc aaagatggtt tgatgaaaaa atttaaaact ggtggcatcg
      481 aaaccttggc atccatttgt cccgaagcat taatcgttcc catcgctatt aaaggctctg
      541 ccgatatggt caaaaaagga atgtttccat taatgccgtt tcacaaatta acatgggaag
      601 ttttatcgcc aatagaaata aaaggtagaa acatcagtga agtggtgcaa gaagccgaaa
      661 attctattcg caacgttgtt gaaggttaat attttgtcaa ctttttttta atctgttgat
      721 ttatctagct tcattcttga aacttaccat tcttttatta aatttgttgc tcttaaaata
      781 aacgaatatg gcagtatcta aaaaagagtt agaattcaat atcaacgacg acgatctacg
      841 cttaaaaatc agtaatttag aaagaacttt agccaaaatc tacgaaggtg gaggtaaaaa
      901 acgcattgaa aaattacacg aacaaggaaa gttaacggca agagagcgta ttgatttgct
      961 tttagaccca gatactgaac gtatagaaat tggtgctttg gctggttatg aaatgtatcc
     1021 agaacacggt ggatgtccat ctggtggagt ggtaattgta attggttatg taaaaggtaa
     1081 acaatgtatt gttgttgcca acgatgcaac ggtaaaagct ggtgcttggt ttccaattac
     1141 aggtaagaaa aacctcagag ctcaagaaat tgcaatggaa aaccgtttgc ctatcattta
     1201 tttggtagat agtgcaggtg tttatttacc aatgcaagat gaaattttcc cagataaaga
     1261 acattttggg cgtattttta gaaacaatgc catcatgagt tcgatgggaa ttgtgcaaat
     1321 tgcagccgta atgggaagtt gtgtggctgg tggtgcctat ttgcctatca tgtcggatga
     1381 atcactgatt gttaacggtt ctggaaccat tttcttggca ggaagttact tagtgaaagc
     1441 tgctattggt gaaaatattg ataacgaaac tttgggtggt gcaactacac atacgtcaat
     1501 ttcgggagtg tgtgattaca aagcagaaaa cgaccaagaa tgcattgcta ctatccgcga
     1561 tttagtggat aaaatcggtc aatctgaaaa tgcaggattt gatagaaaag aagccgttgc
     1621 ccctaaacac gaaccaaaat cggtgtatgg aattatccca accgaccgtt caaaacctta
     1681 caacatgaaa gaagtgttgg agcgcattgt ggacaattca gaatataccg aatacaaagc
     1741 cgattatggt aaaacaatca tttgtgcgta tgctcgcatt gacgggtgga gtgtaggtat
     1801 tgttgccaat caacgtgaag tgatccgctc tgccaaaggc gaaatgcaat tgggaggtgt
     1861 aatttattcc gactcagcag ataaagcagc acgttttatt atgaactgta accaaaaaaa
     1921 tattcctttg gtgtttttgg tggatgtaac cggatttatg gttggttcta aatcagaaca
     1981 cggcggaatt attaaagacg gagcaaaaat ggtaaatgca atggctaatt cggttgtgcc
     2041 gaagtttaca atcgtggtgg gcaactctta tggtgcaggt aactatgcaa tgtgtggtaa
     2101 agcctacgac cctcgtttga ttgtggcatg gccaacagca gaaattgccg taatgggtcc
     2161 tgaacagttg agcggcgtga tggaaatgat tcagcggcag gccaccgaac gttcgggcat
     2221 tccttacgat gagcagcagg gagccatgat ccgcaaccat ctgatgggcg aagtggaaaa
     2281 acaatccaac gcctggtacg cctcctcaca actttgggac gacggagtca ttgacccccg
     2341 agaaacccga aattatctcg gtttctgcct ggccgtggtg aataataaac ccatccaaag
     2401 ttccaatact tacggtgttt tcagaatgta acccaagcga aaaacaagaa aaatgattcc
     2461 acagcaatcc atacatactt tattagtggc caaccggggc gaaattgccc tgcgtatc
//
LOCUS       DAOQVQ010000370         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_50231_length_2517_cov_4.058083, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000370 DAOQVQ010000000
VERSION     DAOQVQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50231_length_2517_cov_4.058083"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2517
                     /gene="sprA"
                     /locus_tag="PKN22_09735"
     CDS             <1..>2517
                     /gene="sprA"
                     /locus_tag="PKN22_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNS43027.1"
                     /translation="DDIFYRQDANTPAHIANDPMVNSDSRTRNVNYQEVFPLQQNQYG
                     TIPNLQLLQLAYYPKERGIYNFDTTNHVDADGFFTNPQDRWGGVMRAMTTTDFELANI
                     EYIQFWVLDPFNKDAEDANPNSLMTGGDLYFNLGNISEDILPDSRKSYENGLAPDGAP
                     APNTMDTTLWAQVPTTQVTVNAFDNDPQARINQDVGLDGFNNAAEKIHFADYINWINA
                     NTTLSPAAKARMIADPSSDDYNYYLDDNYDSQQLSVLDRYKKYNGMEGNSPTPEMYDT
                     MNTKGYSTIGPRLDPDREDINQDNNLQETENYFQYKVSLRPQDMVVGTNYITNRQTFT
                     KGNGDKEYWYQFKIPVRDFTQKINNISDFRSIRFMRMFLKDFDEEVLLRFAKLELVRG
                     EWRRYTQDLSQPGEGVVTDPNLTSFDIGAVNIQENSDRTPINYVIPPGITREIDPSQQ
                     VQRQMNEQSLTLEVCNLQDGDARAAYKNVTFDVRGYKKIKLFVHGESKVDQTPLDNDD
                     LSVFVRLGTDFNENYYEYEIPLKITPFGATVDTDVWPSDNDIEIVFEDLTNLKMKRNR
                     LIEAGNTNVSYNLEYVSSDPNYSNKRIKVKGNPNLQGMKTIMIGVRNPKKQGNSWQDD
                     GMAHCGIVWVNEMRLSDFVSEGGSAAIAQAQIQGADFFNISASASYYGINWGAVDSRV
                     QERQRDQRINANFNSNWQLGQFFGKKFGLSLPFFFGYSVGIINPEYDPYNPDIKLSEY
                     EKSERRRRAKEGQDFQERISYNFTNVRKQLAPGAKPAFWRLANWSASYAYSEDLKRDF
                     NIKYDRTKLWTGNLTYNYSFQAKPWEPFKNVFKKDKLKKSK"
BASE COUNT          867 a          462 c          495 g          693 t
ORIGIN      
        1 gatgatattt tctaccgtca agatgccaat acaccagcgc atattgccaa tgacccaatg
       61 gtaaattcgg atagtagaac aagaaacgta aattaccaag aggtctttcc gttgcaacaa
      121 aatcaatatg gaactattcc aaatttacaa ttgttgcaat tggcctatta tccaaaagaa
      181 cgaggaattt acaattttga cacaaccaat cacgttgatg cagatggttt tttcaccaac
      241 ccacaagacc gttggggagg tgtaatgcgc gcaatgacaa ctaccgattt tgagttagca
      301 aacattgaat acatccaatt ttgggtgtta gaccctttca ataaggatgc agaggatgca
      361 aatcctaatt ctttaatgac aggaggtgat ttgtatttta acttaggtaa tatttcagaa
      421 gacattctac ctgattccag aaaaagttat gaaaatggat tggcaccaga tggagctcca
      481 gctccaaata ccatggatac aacattgtgg gcacaagttc caaccactca agtaacagta
      541 aatgcttttg ataacgaccc acaagctcgc atcaaccaag atgttggttt ggatggtttt
      601 aacaatgcgg cagaaaaaat tcattttgca gattatatca actggataaa tgccaatacg
      661 acactatcac cagctgctaa agcacgtatg attgccgacc cttcttcgga tgattacaac
      721 tattatttgg atgataatta cgattcacaa caattaagtg tcttggatcg ttataaaaaa
      781 tacaatggaa tggaaggcaa ctcgccaact ccagagatgt atgatacaat gaataccaaa
      841 ggttatagca ccattggtcc acgtttagat ccagatagag aagacatcaa tcaagataat
      901 aacttgcagg aaaccgaaaa ttatttccaa tacaaagtca gtttgcgccc acaagatatg
      961 gtggtaggaa caaattacat taccaacaga caaactttta ctaaaggaaa cggcgacaaa
     1021 gaatattggt accagtttaa aattccagtg cgcgatttca ctcaaaaaat taataatatc
     1081 agtgacttcc gctctattcg cttcatgcgt atgttcctca aagattttga tgaagaagta
     1141 ttgttgcgct ttgcaaaatt ggaattagta agaggtgaat ggcgtcgcta tacacaagat
     1201 ttgagtcaac ctggagaagg agttgttacc gaccctaatt tgacaagttt tgatataggt
     1261 gctgtaaaca ttcaagaaaa ctccgacaga acaccaatta attacgtaat tcctccaggg
     1321 attacacgtg aaattgaccc atcacagcaa gtgcaacgtc agatgaatga gcaatcttta
     1381 acattggaag tatgtaattt gcaagatgga gatgctcgtg cagcttataa aaacgtaaca
     1441 tttgacgttc gcgggtacaa aaaaattaaa ttgtttgttc acggtgaatc taaagtggat
     1501 caaactccat tggataacga tgatttatca gtgtttgtga gattaggaac ggatttcaat
     1561 gagaattact acgaatatga aattccatta aaaattacac cattcggagc tacggttgat
     1621 accgatgttt ggccatcgga taacgatatt gagattgttt ttgaagattt gaccaattta
     1681 aaaatgaaac gtaatcgttt gattgaggct ggaaatacaa atgtttccta caacttagaa
     1741 tacgtatcta gtgatcctaa ttattccaat aaacgcatca aagtaaaagg taacccgaat
     1801 ttgcaaggga tgaaaaccat tatgattggt gtgcgcaacc ctaaaaaaca aggtaattct
     1861 tggcaagacg atggcatggc ccattgtggt attgtttggg tgaatgagat gcgtctatct
     1921 gattttgttt cagaaggagg gtcggctgcc atagcgcaag ctcaaattca aggtgctgat
     1981 ttctttaaca tctcggcatc ggcaagttat tatggaatca attggggtgc agtggatagt
     2041 cgtgtgcaag aacgtcaacg cgatcaacgc atcaatgcta acttcaactc caattggcaa
     2101 ttaggacagt tctttggaaa gaaatttggt ctttcattac cattcttctt tggttactcg
     2161 gttggaatta tcaacccaga atacgatccc tacaatccag atattaaatt gtcggaatac
     2221 gaaaaatcgg aaagaagaag aagagccaaa gaagggcaag atttccaaga acgcatttcg
     2281 tataacttta ccaatgtacg taaacaatta gcgcctgggg caaaaccagc attctggaga
     2341 ttagcaaact ggtcggcttc ttatgcttat tctgaagatt taaaacgcga tttcaatata
     2401 aaatacgacc gcacaaagtt gtggacaggt aacctcacat ataactattc tttccaagca
     2461 aaaccgtggg aacctttcaa aaatgtcttc aaaaaagata aacttaaaaa atcgaaa
//
LOCUS       DAOQVQ010000371         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_50541_length_2507_cov_2.976754, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000371 DAOQVQ010000000
VERSION     DAOQVQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50541_length_2507_cov_2.976754"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="rpoA"
                     /locus_tag="PKN22_09740"
     CDS             complement(<1..619)
                     /gene="rpoA"
                     /locus_tag="PKN22_09740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNS43028.1"
                     /translation="MAILDFQKPDKVIMIQSDEQQGQFELRPLEPGYGITVGNALRRI
                     LLSSLEGFAISSIKIKGADHEFTSIKGVVEDVTEIILNLKQVRFKQQIEGTDNEVVTV
                     SISGQDQLTAGDIGKFTSAFQVLNPDLVICNLNKSVKLEIELSIIKGRGYVPAEENKI
                     SGAPFGTIFIDSIFTPIKNVKYAIENYRVEQKTDYEKLVFDIITDG"
     gene            complement(716..1324)
                     /gene="rpsD"
                     /locus_tag="PKN22_09745"
     CDS             complement(716..1324)
                     /gene="rpsD"
                     /locus_tag="PKN22_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNS43029.1"
                     /translation="MARYTGPKTKIARRFRDPIFGADKALDKRPYGPGQHGPAKRRGG
                     KQSEYAIQLAEKQKAKYTYGILERQFENLYKKANAHPGITGEVLLQMCETRLDNTVYR
                     LGIAPTRSGARQLVSHKHITVNGEIVNIPSYQLKAGDVVGVREKSKSLEAITSSLTSN
                     SRKYDWLDWDAASKTGKMISVPAREMIPENIKEQLIVELYSK"
     gene            complement(1404..1790)
                     /gene="rpsK"
                     /locus_tag="PKN22_09750"
     CDS             complement(1404..1790)
                     /gene="rpsK"
                     /locus_tag="PKN22_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNS43030.1"
                     /translation="MAKSNQKKKKNVKVDASGEAHVFASFNNVIVSLTNGAGQVISWS
                     SSGKMGFRGSKKNTPYAALVAAEDAAKEAHDFGLRRVKAYVKGPGAGRESAIRAIHNA
                     GIEVTEIIDVTPIPHNGCRPPKRRRV"
     gene            complement(1847..2212)
                     /gene="rpsM"
                     /locus_tag="PKN22_09755"
     CDS             complement(1847..2212)
                     /gene="rpsM"
                     /locus_tag="PKN22_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNS43031.1"
                     /translation="MARIAGIDLPKNKRGVIGLTYIFGIGRSTASKILKDNNIDENIK
                     VNDWNDDQIGLIREAINAIKVEGALRSEIQLNIKRMMDIGCNRGIRHRAGLPLRGQRT
                     KNNSRTRKGRRKTVANKKK"
     gene            complement(2224..2340)
                     /gene="rpmJ"
                     /locus_tag="PKN22_09760"
     CDS             complement(2224..2340)
                     /gene="rpmJ"
                     /locus_tag="PKN22_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNS43032.1"
                     /translation="MKVRASVKKRTADCKIVKRKGRVYVINKKNPKLKQRQG"
     gene            complement(2375..>2507)
                     /gene="infA"
                     /locus_tag="PKN22_09765"
     CDS             complement(2375..>2507)
                     /gene="infA"
                     /locus_tag="PKN22_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNS43033.1"
                     /translation="HVITAHISGKMRMFYIKILPGDRVKVEMSPYDLSKGRISFRYK"
BASE COUNT          707 a          479 c          391 g          930 t
ORIGIN      
        1 aaccatcagt gatgatatca aaaactaatt tttcataatc agttttttgt tcaacacgat
       61 agttttcgat agcgtattta acgtttttaa ttggcgtgaa gatagaatca atgaaaatgg
      121 ttccaaaagg agcaccactg attttgtttt cttcagccgg aacgtaacct ctacctttga
      181 taatcgacaa ttcgatttct aacttaacag atttgtttaa gttacaaata actaaatctg
      241 ggttcaaaac ttggaatgca gaagtaaatt taccaatatc accagcagtc aattgatctt
      301 gaccagagat agatacagtt acaacttcat tgtctgttcc ttcgatttgt tgtttgaaac
      361 gtacttgttt taagttcaaa ataatttcgg taacatcttc aactacacct ttgattgatg
      421 taaattcatg atctgcacct ttaatcttga ttgatgaaat cgcaaaacct tctaaagaag
      481 aaagtaaaat acgacgtagt gcgttaccta ctgtgattcc gtatcctggc tccaatggac
      541 gcaattcaaa ttgtccttgt tgctcatcag attgaatcat aataaccttg tcaggttttt
      601 gaaaatctaa aattgccatt ttttcttcgt tttaattgcc ttggagttgc gcggttgagt
      661 gaatgaagag ttcactcaac cctttggctc caacacaatg tgttaattta ttttattatt
      721 tagaatacaa ttcgacgatc aattgttctt taatgttttc aggaatcatt tcacgtgctg
      781 gaacagaaat catttttcct gttttagacg ctgcatccca atccaaccaa tcgtattttc
      841 tactgtttga tgttaaagag ctagtaatag cttctaaaga tttagatttt tctctaacac
      901 caacaacatc accagctttt aattggtatg aaggaatatt tacaatttct ccattcactg
      961 tgatgtgttt atgcgatact aattgacgag caccacttct tgtaggagca attcctaaac
     1021 ggtaaacagt attgtctaaa cgagtttcac acatttgtaa caaaacttca ccagtaattc
     1081 ctgggtgagc atttgctttt ttgtataagt tctcaaattg acgctctaaa ataccgtaag
     1141 tgtattttgc tttttgtttt tctgccaact gaattgcgta ttcagattgt tttccaccgc
     1201 gacgttttgc tggtccatgt tgacctggtc cgtatggtct tttatccaaa gctttatcag
     1261 ctccgaagat aggatcacgg aaacgacgtg cgatctttgt ttttggtcct gtgtatcttg
     1321 ccattactat ttgtttttta gaaaagaact gtgaattaag gctttccttc gacagttgct
     1381 ttcttttctg ttattaatta ctactaaact cgacgtcttt ttggaggacg acaaccattg
     1441 tgagggattg gggttacatc aatgatttct gtaacttcga tacctgcatt gtgaattgca
     1501 cgaattgcag attcacgtcc agcacctggc cctttaacat atgctttcac gcgacgtaaa
     1561 ccgaagtcgt gtgcttcttt tgcagcatct tctgcagcaa ctaaagcagc ataaggagtg
     1621 ttctttttag atcctctgaa gcccatttta ccagatgatg accaagaaat aacttgacca
     1681 gctccgttag ttaaagagac aattacattg ttgaaggaag caaatacatg cgcttcaccc
     1741 gatgcatcaa ctttaacgtt cttcttcttt ttttgatttg attttgccat ttttattagc
     1801 tcttaatttt caataatcta ctatactatt caagaatcaa taacgattat tttttcttgt
     1861 tagcaactgt ttttcttcta cccttacgag tacgagagtt attttttgtt ctttgacctc
     1921 ttaaaggaag accagcacgg tgacgaatac ctctgttaca tccgatatcc atcatacgtt
     1981 tgatgttcaa ttgaatttca gaacgcaaag caccttccac tttaatagcg tttattgctt
     2041 cacgaatcaa accaatttga tcatcgttcc aatcattaac tttgatgttt tcatcaatgt
     2101 tattgtcctt taaaatttta gaagctgtgc ttcttccgat accaaagatg tatgttaatc
     2161 caattacacc tcttttgttt tttggtaaat ctatacctgc aatacgtgcc ataattctat
     2221 ttattaacct tgtctttgtt taagttttgg attcttttta ttaatcacat aaactctacc
     2281 ttttctctta acgattttgc aatctgcagt acgtttttta actgatgctc ttactttcat
     2341 tttcttttac tttaaatatt attgagcttc ttaattattt atatctaaat gaaattctac
     2401 cttttgataa gtcataagga gacatttcaa cttttactct atcacctggt aaaattttaa
     2461 tgtaaaacat tctcattttc ccagagatat gagctgtaat tacgtgc
//
LOCUS       DAOQVQ010000372         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_50775_length_2499_cov_3.064239, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000372 DAOQVQ010000000
VERSION     DAOQVQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50775_length_2499_cov_3.064239"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2499
                     /locus_tag="PKN22_09770"
     CDS             <1..>2499
                     /locus_tag="PKN22_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS43034.1"
                     /translation="VSAATNNGIGVSSIGFSCKLMCVKSTNSPSVVSHGYQGITYAAA
                     NGADVINMSWGGPGGGTTGQNVINYAISQGAILIAASGNDNVQTQFYPAAYPGVVSVA
                     ATNSSDQKASFSNYGTWIKISAPGDNILSTTIDNSYGYMSGTSMASPMVAGLVGLMKS
                     LNPTMPNADLINCLYTTAQNINAQNPSYIGKLGAGRIDANAAMACVSASLNLPPVADF
                     SANFTTINAGASIVFTDQSTYNPSSWSWTFAGGTPATYNGQTPPSIVYNTPGTYTVTL
                     TVTNANGSDTETKTNYITVNPPSTCDQLNYPIPSGWTLTSYSAGTGNGYVNGNNIYAD
                     KEKAMYFDASTYSNTQLNAVYILFAKANSSNLNKIVPVKIYDGTAGTPGTLLGTTNLT
                     LGTIRADVLAGAYTEASFVNNVVTLPASKKFFVSVDLTNLSWSADSLSIGSNSNGQTT
                     PSAIWEKQSNNVWYQYGTAGSFNLSASLIMHPFLTSTPTNAVLTASSLSVCEGESITF
                     DAAGSTYEDTLLWSFPGGTPSIVPSAPTATIAYNTPGTYNAILYVVGGGCSLFDSTFV
                     AITVKPNPTISISGTSPICKGASTTLTASGGSTYLWNNGLGTGAVKTVTPDSTTTYIV
                     TGTAANGCDNIAQYQVIVNPLPNVDAGTDLTVCTGNSATLTATGANTYSWNNGLGTGA
                     TKTVSPSSTTQYIVTGTDGNGCVNKDTVIVNVNASLVVSAGSNVTICAGQSTTLTATG
                     ASTYTWDNSLGSGAVKTVSPTVTTTYNVTGTSGGCSSSASVTVTVNPTPTVNAGADVT
                     ICSGQSTTIAATGASSFTWDNGLGAGASHSVSPTS"
BASE COUNT          787 a          488 c          496 g          728 t
ORIGIN      
        1 gtttctgcag ctactaacaa tggaataggg gtttcatcca ttgggtttag ctgtaaattg
       61 atgtgtgtta aatctaccaa ttcaccatct gttgtgtcac acggatacca aggtattacc
      121 tatgctgctg ctaatggagc agacgtaatc aatatgagtt ggggtggacc tggtggagga
      181 acaacagggc aaaatgttat taactatgca attagccaag gtgcaatttt gatagctgcc
      241 tctggtaatg ataacgtgca aacacaattc tatcctgctg catacccagg agtagtttct
      301 gtagcagcaa caaatagttc ggatcaaaaa gcaagttttt ctaactatgg aacatggata
      361 aaaatttcag ctcctggaga taatatttta agcacaacca ttgataattc ttatggatac
      421 atgtcaggaa cttctatggc atctccaatg gtggcaggtt tggttggttt gatgaaatca
      481 ttaaatccta ctatgcccaa cgctgattta ataaactgtt tatatactac agctcaaaat
      541 ataaatgcac aaaatccatc ctatatcgga aaattaggag caggtagaat tgatgcaaat
      601 gctgcaatgg cttgtgtttc tgcaagttta aacttgcctc cagtagccga tttctctgcc
      661 aatttcacga caattaatgc aggtgcttca atcgtattta ccgaccaatc tacgtataac
      721 ccttcaagtt ggtcatggac ttttgcaggc ggtacaccag caacatataa tggacaaact
      781 ccaccttcca tagtttacaa cactcctgga acttatactg taacgctaac cgtaacaaat
      841 gccaacggat cagatacaga aacaaaaaca aattacatca ccgttaatcc accatcaact
      901 tgtgatcagc taaattatcc aattccttct ggttggacgc taacatctta ttctgctggt
      961 actggaaatg gttatgtaaa tggtaacaat atttatgctg acaaagaaaa ggcgatgtat
     1021 tttgatgctt caacttattc taatacgcag ttaaatgccg tttacatttt gtttgcaaag
     1081 gctaattctt ctaacttgaa taaaattgtg cccgtaaaaa tttacgatgg aacggctgga
     1141 acaccaggta ctttgttagg aacaaccaat ttaactttag gaacaattag ggcagatgtt
     1201 ttagcaggag catacacgga agcaagtttt gtaaacaatg tagtaacact tccagcatct
     1261 aaaaaattct ttgtttcggt tgatttaaca aacttatctt ggtctgccga tagcctttca
     1321 ataggaagta attcaaacgg acaaacaacc ccatccgcaa tttgggaaaa acaatctaat
     1381 aatgtttggt atcagtatgg aaccgcaggg tcttttaatt taagtgcatc tttaattatg
     1441 cacccatttt taacaagcac acccaccaat gctgtattaa ctgctagctc actttctgtt
     1501 tgtgaaggtg aatcaataac ttttgatgca gctggaagta cgtatgaaga taccttgttg
     1561 tggagcttcc caggaggaac tccttcaatt gtaccatcag cgccaacagc aacaattgca
     1621 tataatacac caggaacata caatgctatt ttgtatgtag taggaggtgg ttgttcgttg
     1681 tttgactcta cttttgtggc aattactgta aaaccaaatc caacaatttc tattagcgga
     1741 acatctccaa tttgtaaagg agcatcaaca actttaactg cttcgggggg gagtacctat
     1801 ttatggaata acggtttggg aacaggagca gttaaaactg ttacgccaga ttctacaaca
     1861 acctatattg ttacaggaac agccgcaaat ggttgtgaca atatagcaca atatcaagtt
     1921 attgttaatc ctttgcctaa tgtagatgca ggaacagact taacggtttg tacgggaaat
     1981 tcggcaacat taacagccac aggagcaaat acctattcat ggaacaatgg gttggggaca
     2041 ggagctacaa aaacagtttc accatcttca acaacacaat atattgttac aggaacagat
     2101 gggaacggat gtgttaacaa agatacagta attgttaatg tcaatgcttc attagttgtt
     2161 agcgctggct ccaacgtgac catttgtgct ggtcaaagta cgacattaac ggcaactggt
     2221 gcttctactt atacgtggga taattcattg ggtagtggtg ccgttaaaac ggtttcgcca
     2281 acagtaacaa caacctacaa tgttacagga acttctggtg gatgtagctc ttcagcatca
     2341 gttacagtaa ctgttaatcc tactccaacc gtaaatgctg gagcagacgt aaccatttgt
     2401 agcggacaaa gtacaacaat tgctgcaaca ggtgcttcat cctttacttg ggataatggg
     2461 ttgggtgcag gtgcttcaca tagtgtgtca ccaacaagt
//
LOCUS       DAOQVQ010000373         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_51336_length_2483_cov_2.715404, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000373 DAOQVQ010000000
VERSION     DAOQVQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51336_length_2483_cov_2.715404"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKN22_09775"
     CDS             complement(<1..608)
                     /locus_tag="PKN22_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43035.1"
                     /translation="MKYFIPLVALLSLIFTSCSTTKYFDTDDIYSVRTSELPVGESTA
                     DETSYAAFKARKEGKTNDRMTYADEMALINRQNCLEQWRWTSDCGCSYSEWSRYSRFS
                     PYNRYGVMGRNHYMFSNMYAYSSYFPGSSMGMGYYYGHLYHQPTFNSSSWGYYGWGHN
                     MYSPYYNMYDPYYNMYAGVGKYYGPYNPYGFGGYYGMGGYYTL"
     gene            710..2185
                     /gene="proS"
                     /locus_tag="PKN22_09780"
     CDS             710..2185
                     /gene="proS"
                     /locus_tag="PKN22_09780"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNS43036.1"
                     /translation="MSQKYSTREEDYSKWYNETIDRADMAENSGVRGCMVIKPYGYAI
                     WEKIQAQLDKRFKETGHQNAYFPLFVPKSLFEAEEKNAEGFAKECAVVTHYRLKTDDK
                     NPGKLIVDPEAKLEEELVVRPTSEAIIWNTYKGWIQSYRDLPILINQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHTTKQEAYEETERMLEVYAEFAENFMAMPVIKGRKSESERF
                     AGADDTLCIEAMMQDGKALQAGTSHFLGQNFAKAFDVKFSNDEGKQEHVWATSWGVST
                     RLMGALIMTHSDDEGLVLPPKLAPIQVVGVPIFKTDEEYEAIGKKFDELAAELRALGI
                     SFKYDADKNKRPGWKFAEYEAKGVPVRLAMGPRDMQNGNIEVARRDTKTKEVVSFDGL
                     ANFIQNLLNEIQNNLFQKALAFRNENLHQVDTYEEFKTQLAQKGGFYLVHWDGTAETE
                     AKIKEETQATIRCIPFEASEPGVCMVTGKPSKQRVVIAKAY"
     gene            2187..>2483
                     /locus_tag="PKN22_09785"
     CDS             2187..>2483
                     /locus_tag="PKN22_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43037.1"
                     /translation="MTQKKSDKSMDLNSQQIVNLLLEKACLKQDVYAYSLNAFNEFKD
                     LVRSEIDLLKTKVDDTRIRLSIVERGQNEFHTYVGSDVLVYQVHTNIFRLPDEHP"
BASE COUNT          865 a          418 c          510 g          690 t
ORIGIN      
        1 agtgtataat atcctcccat tccgtaataa ccgccaaaac cataaggatt ataagggcca
       61 taatattttc ctactcctgc atacatgtta taatatggat catacatatt gtaataagga
      121 gaatacatgt tgtgtcccca tccataatac ccccaagaag atgaattaaa agtaggttgg
      181 tggtatagat gtccataata ataacccatt cccatagagg aaccagggaa ataacttgaa
      241 taagcataca tgttgctaaa catataatga tttcttccca ttacaccata tctattgtag
      301 ggcgagaagc ggctgtaacg tgaccattca gaatacgagc aaccacaatc actggtccaa
      361 cgccattgtt ccaaacaatt ttgacgattt atcaaagcca tttcatcggc ataagtcatt
      421 cgatcgtttg tttttccttc tttacgtgct ttgaaagctg catacgaagt ttcgtcggct
      481 gtagattcac caactggcaa ctcactagtt cttacagagt aaatgtcatc tgtgtcaaaa
      541 tattttgttg ttgaacaaga tgtgaaaatc aagctcaata atgcgactaa tggaatgaaa
      601 tatttcatag ctacgtaaat tatactttaa aagtacaatg aaatttataa atttgcaaat
      661 cacaagtttg aatttaaaca cattttaaca aataaatttt cagaatagta tgtctcaaaa
      721 atattctaca agggaagagg attattcaaa atggtacaac gaaaccattg atagagcaga
      781 tatggcagaa aactcagggg taagaggttg tatggttatc aaaccgtatg gctacgcaat
      841 ctgggagaaa atccaagcgc aactcgacaa acgttttaaa gaaacagggc accaaaatgc
      901 ttacttcccg ttgtttgttc ccaaaagttt atttgaggca gaagaaaaaa atgcagaagg
      961 ttttgccaaa gaatgtgctg ttgttaccca ttatcgttta aaaacagacg ataaaaatcc
     1021 agggaagtta atagttgatc cagaagcaaa attggaagaa gaattagtag tgcgtccaac
     1081 atcagaagcc atcatttgga atacctacaa aggttggatt caatcgtatc gcgatttacc
     1141 aatccttatc aatcagtggg caaatgtggt gcgttgggaa atgcgtacac gcttgttttt
     1201 acgtactgcc gaatttttat ggcaagaagg ccatacagct catactacaa aacaagaagc
     1261 ctatgaagaa acagaacgta tgttggaagt gtatgcagaa tttgcagaaa actttatggc
     1321 catgcctgtt atcaaaggta gaaaatcgga aagtgaacgt tttgctggag ctgatgatac
     1381 gttatgcatt gaagcaatga tgcaagatgg aaaagcgttg caagcaggaa cttctcattt
     1441 cttgggacaa aattttgcta aagcgtttga tgtgaaattt agcaacgatg aaggaaaaca
     1501 agaacacgtt tgggcaactt catggggtgt ttcaacacgt ttaatgggag cgttaatcat
     1561 gacgcattca gacgacgaag ggttggtgtt gcctccaaaa ttggcaccta tacaagttgt
     1621 gggtgtgcct atttttaaaa ccgatgaaga atacgaagca atcggtaaaa agtttgacga
     1681 attggctgcc gaattaagag ctttgggcat ttcgtttaaa tacgatgcag ataaaaataa
     1741 acgtccggga tggaaatttg cagaatacga agctaaaggt gttccggtgc gtttggcaat
     1801 gggacctcgc gatatgcaaa acggaaacat tgaagtagct cgccgtgata ccaaaacaaa
     1861 agaagttgta agctttgacg gattggctaa ttttattcaa aatttattga atgaaattca
     1921 aaacaacttg ttccaaaaag cgttggcatt tagaaatgaa aatttacacc aagtagatac
     1981 ttacgaagaa tttaaaacgc aattggcaca aaaaggtggt ttttatttag tgcattggga
     2041 tggaactgct gaaacagaag caaaaattaa agaagaaaca caagcaacca ttcgttgtat
     2101 tccttttgaa gcatcagaac caggtgtttg tatggtgaca ggaaaaccgt caaaacaacg
     2161 tgtagttatt gcaaaagcat attaaaatga cccaaaaaaa gtcggataaa tccatggatt
     2221 tgaattccca gcaaattgta aaccttttgt tggaaaaagc ttgcttaaaa caagatgtgt
     2281 atgcatattc actcaatgct tttaacgagt ttaaagattt ggtgagaagt gaaattgatt
     2341 tactgaaaac aaaggttgat gatacgcgca ttcgcttgtc gattgttgaa cgggggcaaa
     2401 atgaatttca tacctatgtt ggaagcgatg tgttggtgta tcaagtgcat accaatattt
     2461 tccgtttacc cgacgaacat cca
//
LOCUS       DAOQVQ010000374         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_51385_length_2481_cov_5.014015, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000374 DAOQVQ010000000
VERSION     DAOQVQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51385_length_2481_cov_5.014015"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PKN22_09790"
     CDS             <1..520
                     /locus_tag="PKN22_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43038.1"
                     /translation="IERQHYAFKNFSNFDEKSWNELYQKAVETSRSVRKLPVQIIGSD
                     ISSEMMLKAKRNLRRLSIGRFVEVSTQSFDEVKNPGFEKGMVITNPPYGERMGDNIPD
                     LYARIGDWMKTEMQGFDCWLISSSEEGLKTVGLRPEAKIKLFNGSLECSFRKYSIYAG
                     SKRDQYQNNESK"
     gene            517..1632
                     /locus_tag="PKN22_09795"
     CDS             517..1632
                     /locus_tag="PKN22_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS43039.1"
                     /translation="MNVLIVNTSTGWGGLEMNVVKLATELQQHGIRIFMVCQENTTFA
                     QKIEGKFECVLQLKKVKKYFDFNNAQVVANYCKTNQITKVFTAFRPDLDLLMWAKRKN
                     KALKIIHQQQMQIGIPKKGFFQRLRFKAVDLWLTPLEWLRQEALEKTTLAAEKIRIVP
                     LGVRKEPFLQEQPSRQAAQQFFGFQSDAFVIGVLGRIDEKKGQLFLVQTLKKLIDTGE
                     NVFLLIVGNPTVDDPKGALYYEKVKQYIAKNQLEKKVLFAPSTNEVLQFYAAIDLFVM
                     SSEGETFGMVTVEAQFSKTPVIGTNTSGTPEVLGHGTRGDLYSFNNEASFIAAYYSLR
                     KKMEEGQLDLTAIQQEAMDNYSLDKEVAGVLAALNEV"
     gene            complement(1735..1807)
                     /locus_tag="PKN22_09800"
     tRNA            complement(1735..1807)
                     /locus_tag="PKN22_09800"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1772..1774),aa:Phe,seq:gaa)
     gene            complement(1911..>2481)
                     /locus_tag="PKN22_09805"
     CDS             complement(1911..>2481)
                     /locus_tag="PKN22_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNS43040.1"
                     /translation="FDEHREGLTLGEGAAYLVLEAEDICSNKNKLALVSGFGNANDAF
                     HPSATSDDARGPIKAMKDALLSANLEPSAIDYINAHGTGTVNNDETESFAFIELFGKN
                     IPPFSSTKAYTGHTLAAAGALEAIFCVLSIQHGELYPNLHFEQPIASRGLIPVTNFQQ
                     DKEINHVLSNSFGFGGNGTSLLISKVLEQ"
BASE COUNT          826 a          436 c          496 g          723 t
ORIGIN      
        1 tattgagcgt cagcattatg catttaaaaa tttctccaat tttgatgaaa aatcgtggaa
       61 tgagctttac caaaaagcag tagaaacatc ccgttctgta cgcaaattac cagtgcaaat
      121 catcggttcc gacattagtt ctgaaatgat gttaaaggct aaaagaaatt taagaagatt
      181 gagcataggc cgttttgttg aggtgagcac gcaatcgttt gatgaagtta aaaaccctgg
      241 ttttgaaaaa ggaatggtta ttaccaaccc gccttatggt gagcgtatgg gcgataacat
      301 cccagattta tatgcacgca ttggcgattg gatgaaaacc gaaatgcaag gttttgattg
      361 ctggttgatt tcttctagcg aggaaggatt gaaaacggta ggtttacgac cagaagcaaa
      421 aatcaaattg tttaacggta gtttggaatg tagttttaga aaatatagca tttatgcagg
      481 ttcaaaacgc gaccaatacc aaaacaacga aagtaaatga acgttttaat tgtaaacaca
      541 tcaacaggat ggggagggtt ggaaatgaac gttgtcaaat tggcaaccga attgcaacaa
      601 cacggcattc gtatttttat ggtgtgtcaa gaaaatacca cttttgctca aaaaattgag
      661 ggcaaatttg agtgtgtttt acaattaaaa aaagtcaaaa aatactttga tttcaacaat
      721 gcgcaagtag tagctaatta ttgcaaaacc aaccaaatta caaaagtttt tactgctttt
      781 cgtcctgatt tagacctgct tatgtgggca aagcgtaaaa acaaagcgtt gaaaatcata
      841 caccaacaac aaatgcaaat tggtattccc aaaaaaggat tttttcagcg tttgcgtttc
      901 aaagcagttg atttgtggtt gacacctttg gaatggttgc gacaagaagc tttggaaaaa
      961 actacacttg cagcagaaaa aattagaata gttcctttag gtgtgcgcaa agaaccattt
     1021 ttgcaagaac aaccctctag acaagcagca caacaatttt ttggatttca atccgatgca
     1081 tttgtaattg gcgttttagg gcgaattgat gaaaaaaaag gacaattgtt tttggtgcaa
     1141 accttaaaaa aactcataga tacaggtgaa aatgtctttt tactcattgt aggcaatccc
     1201 actgttgacg atccaaaagg agctctctat tatgaaaagg ttaagcagta cattgccaaa
     1261 aatcagctag aaaaaaaggt attgtttgca ccctccacca acgaagtatt gcaattttat
     1321 gctgccattg atttgtttgt tatgtcaagc gaaggcgaaa catttggaat ggtaaccgta
     1381 gaggcgcaat tttccaaaac tccagtgata ggaaccaaca ccagtggtac acccgaagtt
     1441 ttaggacatg gcacacgtgg tgatttatac agtttcaata acgaagcgtc ttttattgca
     1501 gcgtattatt ctttgcgcaa aaaaatggaa gaagggcaat tggatttaac agccattcaa
     1561 caagaagcaa tggataatta ctcacttgat aaggaagttg caggagtatt ggcagcgttg
     1621 aacgaagttt aactcaaatt tatgcttagt ccccaacttt tgaacttgat ccgtaataat
     1681 tcatagtagt ttgtttaaac aaaaaaagcg aggtaaaaac ctcgctttct aatgtggtgc
     1741 ctccaggaat cgaaccaggg acacaaggat tttcagtcct ttgctctacc aactgagcta
     1801 aggcaccagc cttatttgag gtgcaaaaat aaagctattt tttaaaacaa caagtgtttt
     1861 tatgaaaaaa atgaatttta tttttgaaac taattgtttt aagatgcaaa ttattgttct
     1921 aaaactttgc taatcaataa agaagtgcca ttaccaccaa aaccaaacga attggataac
     1981 acatgattta tttctttgtc ttgctggaaa ttcgttacag gaatcagtcc tctgctggca
     2041 ataggttgct caaaatgcaa gttgggatac aattcaccgt gctgaatact taatacacaa
     2101 aaaattgctt ccaaggctcc agcagcagca agtgtgtgac ccgtataggc ttttgtggaa
     2161 ctaaacggtg gaatgttttt gccaaacaac tctataaacg caaacgattc ggtttcatca
     2221 ttgttgacgg tgcctgttcc atgtgcattg atgtaatcaa tagctgatgg ttctaaattg
     2281 gcagacaaaa gtgcatcttt cattgcttta attggtccac gggcatcgtc tgacgttgca
     2341 gatgggtgaa aagcatcgtt ggcatttcca aatccgctga caagtgccaa tttatttttg
     2401 ttactgcaaa tatcttcggc ttctagaacc aaataagcag ctccttctcc caaagttaaa
     2461 ccttctcggt gttcgtcaaa t
//
LOCUS       DAOQVQ010000375         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_51389_length_2481_cov_3.637675, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000375 DAOQVQ010000000
VERSION     DAOQVQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51389_length_2481_cov_3.637675"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKN22_09810"
     CDS             complement(<1..923)
                     /locus_tag="PKN22_09810"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNS43041.1"
                     /translation="MKSLGATISTHFLIFALCFPFISCSQQVDSTLYTNDNDTATIDS
                     VYYNSIYDPAYYKKFDNTWVNLEDGLDYIEMDGPRKSKIGDSKISLLRINPEKFKFEM
                     LSASQFDSTSKCIVDWAEHHDLMVAINASMYDMRRQLSSKAYMRNTLEYANNPKPYEG
                     YNMAIAFNPLRKGLSEFDIIDLKCTSLETVKKDYASIAQGLRMIDCNGTPMSWNKNPQ
                     WCSQLIVAKDDYQQIYFVFTRSPYSQNEMIRFMTQLERPLRNAIYMEGGPQTSLYVDT
                     ETERIEKLGSYVSNTYPITTNAEFWKLPNVIG"
     gene            complement(990..1763)
                     /gene="rsmA"
                     /locus_tag="PKN22_09815"
     CDS             complement(990..1763)
                     /gene="rsmA"
                     /locus_tag="PKN22_09815"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNS43042.1"
                     /translation="MNVKAKKHLGQHFLVDKNICQKIADQFQYHQGCTRALEIGPGTG
                     ALTEYLLKQPQTDLTVMDVDTESIAYLNIHFPQLEGKILEADFLKEDLTKIMGTTPFA
                     VVGNFPYNISSQILFKCLDYRNQIPEIMGMFQKEVAERVAEKPGSKQYGILSVLMQAF
                     YDITYCFTVSENVFNPPPKVKSGVIRCTRNSRTELPCDEKLFIQVVKTGFNQRRKTLR
                     NSLKKLTPIGFEHELLTLRPEVLSVEQFIQLTLAIQNFN"
     gene            complement(1760..2074)
                     /locus_tag="PKN22_09820"
     CDS             complement(1760..2074)
                     /locus_tag="PKN22_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNS43043.1"
                     /translation="MIIYNVTVNIDLSVHDEWLEWMRSKHIPDVMSTGCFIESKISRV
                     HGEEEGGATYAIQYIAPNQDQLDVYQQLFAPKLQHEHSAKYQGKFGAFRTILTIVDEF
                     KK"
     gene            2224..>2481
                     /locus_tag="PKN22_09825"
     CDS             2224..>2481
                     /locus_tag="PKN22_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNS43044.1"
                     /translation="MVKIRDIELGDFPLLLAPMEDVSDPPFRALCKENGADLMYTEFI
                     SSEGMIRDAAKSVQKLDIFEYERPIGIQIFGSEIETMRQCAE"
BASE COUNT          751 a          437 c          465 g          828 t
ORIGIN      
        1 cctatcacgt tgggtaattt ccaaaactct gcattggtag taataggata agtatttgat
       61 acatagctac ctaatttctc aatacgttcg gtttcggtat ccacatataa actagtttgt
      121 ggtccgcctt ccatgtaaat agcatttcgc aaaggacgtt ctaactgtgt cataaatcgt
      181 atcatttcgt tttgagagta gggagaacga gtaaatacga aataaatctg ctggtaatcg
      241 tctttggcaa caattaattg gctgcaccat tgtggatttt tattccaact cataggtgtg
      301 ccgttgcaat caatcatccg aagaccttgc gcaatggaag cataatcttt tttaaccgtt
      361 tctaagctgg tgcattttaa gtcaattatg tcaaattctg acaatccttt tctcaatgga
      421 ttaaatgcaa tagccatgtt gtaaccctca tatggcttag ggttgttggc atattccaat
      481 gtgttgcgca tataggcttt gctcgaaagt tggcgacgca tatcgtacat actagcgtta
      541 atggctacca tcaaatcgtg gtgttctgcc caatctacaa tgcatttgga agtagaatcg
      601 aattgtgaag cagaaagcat ttcaaatttg aatttttctg gattgatgcg caataaacta
      661 attttagaat cgccaatttt tgatttgcgg ggtccatcca tttcaatgta atccaaacca
      721 tcttctaagt tgacccatgt gttatcaaac tttttatagt aagcagggtc ataaatggaa
      781 ttataataca ctgaatctat tgttgccgta tcattgtcgt ttgtgtaaag tgtagaatct
      841 acttgctgcg aacaagatat aaacggaaaa cacaaagcaa atattaaaaa atgcgtgcta
      901 attgttgcgc ctaaagattt cattttggag agataagttt tgttaaattt aattcaaata
      961 ttaatgtgat caaatacgat aacaaatttt taattaaaat tttgaatggc caatgtgagt
     1021 tgaataaatt gttccacact caaaacttct ggtcgcaaag tcaacagctc atgttcaaat
     1081 ccaattggtg taagcttttt caatgaattt ctcaatgttt ttctacgttg attgaagcct
     1141 gtttttacaa cctgaataaa tagtttttca tcacatggaa gttctgttct actgttgcgg
     1201 gtgcaacgta taacacctga ttttactttt ggtggaggat taaacacatt ctcgctcact
     1261 gtaaaacaat aggtgatgtc ataaaatgcc tgcattaaaa cacttaaaat tccatattgt
     1321 ttagaacctg gtttttcggc tacacgttcg gctacttctt tttgaaacat gcccatgatt
     1381 tctggaatct gattgcggta atccaagcat ttaaaaagta tttgcgacga aatgttatag
     1441 gggaaattac ccaccacagc aaaaggcgta gtgcccatta tttttgttaa atcttctttt
     1501 aaaaagtcgg cttctaaaat ttttccttcc aactgtggaa agtgaatgtt taaatatgca
     1561 atggattcgg tgtcaacatc cattaccgtt aggtcggttt gtggttgttt taataaatat
     1621 tcggtgagag cgccggttcc aggaccaatc tccaaagcac gtgtgcatcc ttgatgatac
     1681 tgaaattggt cggcaatttt ttggcaaatg tttttatcca ccaaaaaatg ttgtcccaag
     1741 tgttttttag cttttacgtt cattttttaa attcgtcaac aatggttaat atggtacgaa
     1801 aagcgccaaa tttcccttgg tatttagccg aatgttcgtg ttgcaatttg ggagcaaaaa
     1861 gttgctggta aacatccaat tggtcttgat tgggcgcaat gtattgaata gcatacgtgg
     1921 cgccaccttc ttcctcacca tgcactcgtg aaattttact ttcaataaaa caacctgtgc
     1981 tcatcacatc aggaatgtgt tttgaacgca tccattccaa ccattcgtcg tgcacagaaa
     2041 gatcaatatt tactgtaacg ttataaataa tcatgctaca aaattacaca aacaaacgtt
     2101 tttttaggga tttttctgag gattttccta aaatacaaat ggtgatattc caattaatgg
     2161 aaataatgga atattagcaa gaatggttat caatttaaaa ttcatcctta cctttgcagt
     2221 caaatggtaa aaattcgcga tatagaattg ggagatttcc cactgttgct tgctccaatg
     2281 gaggatgtga gtgatccacc gtttcgtgca ttgtgcaagg agaatggagc agatttgatg
     2341 tacaccgaat ttatttcatc cgaaggaatg attcgcgatg cagcaaaaag tgttcaaaag
     2401 ttggatattt ttgaatacga acgacctatt ggaattcaaa tttttggttc tgaaattgaa
     2461 accatgcgcc aatgtgctga a
//
LOCUS       DAOQVQ010000376         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_51499_length_2477_cov_2.907102, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000376 DAOQVQ010000000
VERSION     DAOQVQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51499_length_2477_cov_2.907102"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="PKN22_09830"
     CDS             complement(<1..1721)
                     /locus_tag="PKN22_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS43045.1"
                     /translation="MLSIGLAIWLYSLKNNGWVSELPKKWRYFLIGLRSFSLFLTILL
                     LFGIVFQAFKYKKEKPIIVLGIDNSASMLNYSDSASIKKLTNQLIEEAKNTLADKFEV
                     LTYNYSGTPSVLVENVQFNATSTDLSTAIENTRSEFYNRNLAGIVLFSDGNFNTGLNP
                     VYAAEKLTTTSIYSLGVGDTIAKRDQLIKHITHNDIAFINNEFPVVVDVEGIKMGKTE
                     SEVIVELNGKKIASQRAKFKDGSADYEQINFLLNANSPGIHRYVVKLAHQQNEYNYSN
                     NEQAFYVEVIDSRSKVVLLSSAPHPDIAALKSVWDKDQNLEVKFHSLTDWNRDLKNVD
                     LIVWHEPGLNTSKENIQAIMNNSISKLFIVGSQSNASSIRQLKIGVEIPSSNNLDDVE
                     GSFNKGFNLFEFSDKLSKAFDYYPPLKAKFGKLIIPNNAQPLTFQRIGPVVKKDAQLF
                     FANAQSEQGKSYKYGLVFGEGLWRWKLTEFGKTQATEAFDELFSKIGQYLLVKQNTEP
                     FRVTLPTSFSETKAVIVDATVLNQSLEPITTSTVNFELENEAKQLSKFQFGVVGNGYK
                     LDLGQLKA"
     gene            complement(1840..2196)
                     /locus_tag="PKN22_09835"
     CDS             complement(1840..2196)
                     /locus_tag="PKN22_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNS43046.1"
                     /translation="MKTIQLKRVYENFDNTDGFRILVDRLWPRGIKKENAHIDLWLKE
                     IAPTTELRTWYNHEVEKWNEFQQCYVAELNANKPVQQELLEAIQQHKKITLLYGAKDT
                     QHNQAVVLKDWLSHYL"
     gene            complement(2256..>2477)
                     /locus_tag="PKN22_09840"
     CDS             complement(2256..>2477)
                     /locus_tag="PKN22_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43047.1"
                     /translation="QPIVVYPNPVQTKLFIQTQAELVRVRVIDVTGKEIAVNRDENSI
                     NVAHLSDGYYQLLIETTKGNYQTKFIKNK"
BASE COUNT          774 a          449 c          398 g          856 t
ORIGIN      
        1 gcttttagtt gacctaaatc caatttataa ccgtttccaa caacaccaaa ttggaatttt
       61 gataattgtt tagcttcgtt ttctaattcg aaattaacag ttgatgttgt tataggctcc
      121 aatgattgat tcagtaccgt ggcatcaaca attaccgctt ttgtttctga aaaagaagta
      181 ggtaatgtta ctctaaaagg ttctgtattt tgtttaacca ataaatactg accaattttt
      241 gagaacaatt catcaaatgc ctctgttgct tgtgtttttc caaattcggt taacttccaa
      301 cgccacaaac cttcaccaaa taccaatccg tatttatacg atttcccttg ctcactttgc
      361 gcattggcaa aaaacaattg ggcgtctttc ttcacaactg gaccaatacg ttgaaaagtc
      421 aaaggttgag cattgttagg tattataagt tttccaaatt ttgcttttaa tggtggataa
      481 taatcaaacg cttttgaaag tttatcgcta aattcaaata agttaaatcc tttgttaaaa
      541 cttccttcaa cgtcgtctaa attattgcta gatggtattt caacgcctat tttaagttgt
      601 cgaatactac ttgcattgga ttgtgaacca acaataaaca attttgaaat agaattgttc
      661 ataatggctt gaatgttttc tttagaagtg ttcagcccag gctcgtgcca tacaattaaa
      721 tcaacatttt ttaaatctct attccaatca gttaaagagt ggaatttcac ttctaaattt
      781 tggtctttat cccaaaccga tttcaatgcg gcaatgtctg gatgtggtgc cgaagaaaga
      841 agaacaactt tgcttctgct gtcaataact tccacataaa acgcttgctc gttgttggag
      901 taattgtatt cgttttgttg gtgagctaat tttacaacat aacgatgaat tccaggtgaa
      961 ttggcgttca ataaaaaatt aatttgctca taatcggcac taccatcctt gaattttgca
     1021 cgttgcgaag caattttttt tccgtttaat tcaacaataa cctcgctttc agtttttccc
     1081 atcttgatgc cctcaacatc cacaactact ggaaattcat tatttataaa agcaatatca
     1141 ttgtgtgtga tgtgtttgat gagctgatcg cgtttagcaa tagtatcgcc aacaccaagt
     1201 gagtaaatgg aagttgttgt tagtttttca gctgcataaa ctggattcaa tcctgtattg
     1261 aaatttccgt cagaaaataa aacaatacca gccaaattgc ggttgtaaaa ttcactcctt
     1321 gtgttttcaa tggcagtaga taaatcagta gaagttgcat taaattgaac gttttcaacc
     1381 aaaacagaag gagttcctga ataattatag gtcaagactt caaatttatc ggctaaggtg
     1441 ttttttgcct cttcaatcaa ttggttggtc agttttttta tgcttgccga atcggaatag
     1501 ttgagcattg aagcactgtt gtcaattcct aaaacaatta ttggtttttc ttttttgtac
     1561 ttaaatgctt gaaaaacaat accgaataac aataaaatgg taagaaaaag cgaaaaactt
     1621 cgtaatccga ttaaaaaata gcgccatttt ttaggtaatt cgcttaccca accgttgttt
     1681 tttagtgaat acaaccaaat tgccaaacca atgctaagca aaaggcaagg taacaaccac
     1741 cacaacgata tttcagagct aaaattcatt cagactccga aaatacaaaa agaataataa
     1801 atggaatgcg tttgggctca aatatgcaca aatgaatttt tacaggtaat ggctcaacca
     1861 atcttttagc accacagctt gattgtgttg agtgtctttt gccccataaa gcaaggttat
     1921 ttttttatgt tgttgaatcg cttctaacaa ttcttgttga actggtttat tagcatttaa
     1981 ctcagcaaca taacactgtt gaaattcatt ccatttttcc acttcgtgat tataccacgt
     2041 tcgcaactct gttgttggag caatttcttt taaccacaaa tcgatgtgtg cattttcttt
     2101 tttgattcca cgcggccaaa gacggtcaac caaaatccga aaaccatcag tgttgtcaaa
     2161 attttcgtaa acacgtttta gttggattgt tttcattgta aaatgattta acaccgaaat
     2221 ttacaataaa tactcaccaa atcaaaacct attaactatt tatttttgat gaatttggtt
     2281 tggtaatttc cttttgttgt ttcaatcaat aattggtaat aaccatcgga taaatgtgcc
     2341 acattgatgc tgttttcatc gcgatttacc gcaatttctt ttcctgttac atcaattacc
     2401 cgaacacgca ccaattcagc ttgggtttgg ataaataatt ttgtttgaac aggattagga
     2461 taaacaacaa tcggttg
//
LOCUS       DAOQVQ010000377         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_51533_length_2476_cov_3.723255, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000377 DAOQVQ010000000
VERSION     DAOQVQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51533_length_2476_cov_3.723255"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKN22_09845"
     CDS             complement(<1..617)
                     /locus_tag="PKN22_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS43048.1"
                     /translation="MDEFNKKLLETLYGMITPSKVEMFERLAPQKTNYLTVAMENIYQ
                     EHNASAVMRTCESLGIQSLHVIEKDWKYQAQRDIARGAGRWIDTFNYSDATPTLSCIE
                     QLKADGYQIVATTPHKDDFTIDTLPIDQPIALFFGTEGEGISQDIISNADRFVKIPMH
                     GFTESLNVSVSVAIAAYTLRKRIEQSNVNWQLSEDELTALKIKWCT"
     gene            complement(684..1547)
                     /locus_tag="PKN22_09850"
     CDS             complement(684..1547)
                     /locus_tag="PKN22_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS43049.1"
                     /translation="MRVLITFLFVVGWSFFTSSQSLNGLWKGSLYPPKSSVDKGMLIY
                     LQINEDKSGAITGKVRNERFNTNDFALKNIKGNKKTLDVSINETVISQKAVGSRTTWC
                     RLDLTLKYNPETGYLVGTYSSSDCRNQVGNVILYRDPVDFPGDKENPESQHWFELLAQ
                     DIGKGLNAPEIRKIERDNFVFQPIYFDYDKAEIKPEFYDFLNHLVKIVEGHSDLRVKV
                     TGHTDSDGSDAYNDELSKRRAQAIIDFFVARGLKADRLEFDFNGEHKPVDSNKTPEGR
                     QKNRRVEFSFI"
     gene            complement(1600..>2476)
                     /gene="mltG"
                     /locus_tag="PKN22_09855"
     CDS             complement(1600..>2476)
                     /gene="mltG"
                     /locus_tag="PKN22_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNS43050.1"
                     /translation="LEDKKAFLKEVEKQNLTNQTFDAGKYVILSGTLVKDLVNGFSRG
                     ENGHGLAEVKVNVLFNRSKTIEDIGMNISQCIEADSASIVDYITAPSTLEKYHFTREQ
                     IPALFIPDQYELYYDTNAEEFVAFMAEEFKKFWTEERKEKMKAIGLTSPSQVATLTSI
                     VYSEQGKVTAEWPIIARLYLNRIAKGMKLQSDPTFKFCWGEKLKDTERLLAIHRDIDC
                     KYNTYKINGLPPGPICLVPQKAIDAVLNPDSNNYLFMCAKPDYSGEHNFTHSGDVHVN
                     NARIYQRWLSKEGKH"
BASE COUNT          705 a          483 c          447 g          841 t
ORIGIN      
        1 gtgcaccact ttatttttaa agcagttaac tcgtcttctg acagttgcca attgacgtta
       61 gactgttcaa ttcgcttgcg caaggtgtag gctgcaattg caacagaaac ggatacattt
      121 aaactttcgg taaaaccatg cattggaatt tttacaaacc tgtcggcatt ggaaataata
      181 tcttgagata ttccttcacc ttctgttcca aaaaacaaag caatgggctg atcgatgggt
      241 aaggtgtcaa tcgtgaaatc gtctttgtgt ggagtagtag caacaatttg atagccgtct
      301 gcttttaatt gctcaatgca actcaaggtt ggggtagcat cgctgtaatt aaaagtgtct
      361 atccatcttc ccgcaccacg agcaatgtcg cgttgtgctt gatatttcca atctttttca
      421 ataacatgta ggctttgaat tcccaaactc tcgcaggtgc gcatcaccgc cgaagcattg
      481 tgctcttggt aaatgttttc catcgcaaca gtgaggtaat tggttttttg tggcgctaaa
      541 cgttcaaaca tttcaacctt gctgggagta atcattccat ataaggtttc caataatttt
      601 ttattgaatt catccatgag acaaatataa cgagaattta caacctactt ggctgaaaaa
      661 cgtattgtaa aatgattaaa aaattagata aaactaaatt ctacacggcg gtttttttgt
      721 cgcccctcgg gagttttgtt tgaatccaca ggtttgtgct cgccattaaa gtcaaattcc
      781 aagcgatcgg ctttcaatcc acgagcaaca aaaaaatcaa taatggcttg tgcacgacgt
      841 ttggaaagtt cgtcgttgta tgcgtcagat ccgtcagaat cagtgtgtcc ggttactttt
      901 actcgtaaat ccgaatgtcc ttccactatt ttcactaaat gatttaaaaa atcgtaaaat
      961 tcgggtttaa tttccgcttt gtcgtagtca aaataaatgg gttgaaaaac aaaattgtcg
     1021 cgttcaattt ttcgaatttc gggagcattg agtcctttgc caatgtcttg cgccaacaat
     1081 tcaaaccaat gttgcgattc tggattttct ttatcgccag ggaaatccac aggatctctg
     1141 tataaaatca cattgcccac ttgatttctg caatcactac ttgaataggt gcccaccaaa
     1201 tatcctgttt cgggattgta ttttaaggtt aaatccaacc ggcaccaagt tgttctactt
     1261 cctactgctt tttgagaaat gaccgtttcg ttgatgctta catccaaagt ttttttattg
     1321 cctttaatgt tttttagtgc aaaatcgttg gtattgaagc gttcgtttct aacttttccg
     1381 gttattgcac cactcttgtc ttcgttaatt tgaagataaa tcaacattcc tttatccaca
     1441 cttgattttg gaggatacaa acttcctttc caaagtccgt ttaaactttg tgacgaagta
     1501 aaaaaactcc aaccaacaac aaataggaat gtaattagaa ctctcattgt tcttttattc
     1561 gtaaaaaaac aaaaaatgtt ccgtttttaa gtaaatttcc taatgtttac cttcttttga
     1621 taaccaacgt tggtaaatac gtgcattgtt gacatgtaca tctcccgaat gggtgaagtt
     1681 atgttctccc gaataatctg gttttgcaca cataaacaaa taattgttgc tatcagggtt
     1741 caatacggca tcaattgctt tttgaggaac caagcaaatt ggaccaggag gcaagccgtt
     1801 gattttataa gtattgtatt tgcaatcaat atcacgatga attgcaagca agcgttctgt
     1861 atctttgagt ttttcgcccc aacaaaattt aaacgttgga tcggattgta atttcattcc
     1921 tttagcaatt ctattcaagt ataaacgagc aataatgggc cattctgcag ttacttttcc
     1981 ttgttcagaa taaacaatac tggttagtgt tgcaacttgt gaaggagacg ttaacccaat
     2041 tgctttcatt ttttctttac gttcttctgt ccagaatttt ttaaattcct cggccataaa
     2101 tgccacaaat tcctcggcat tggtatcgta atacaattcg tattgatcgg ggatgaataa
     2161 ggctggaatt tgttcgcgtg taaaatggta tttttccaat gttgatgggg cggttatgta
     2221 atcaacaatg gatgcgctat cggcttcaat gcactgagaa atgttcatgc cgatgtcttc
     2281 aatagtttta cttctgttaa aaagtacgtt tactttaact tcagcaaggc cgtgcccatt
     2341 ttcgcccctt gaaaaaccat tcactaaatc ctttacgagg gtgccagaaa gtattacata
     2401 ttttccagcg tcaaacgttt gattggttaa attttgtttt tcaacttctt tcaaaaacgc
     2461 ttttttatct tccaat
//
LOCUS       DAOQVQ010000378         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_52084_length_2459_cov_4.200915, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000378 DAOQVQ010000000
VERSION     DAOQVQ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52084_length_2459_cov_4.200915"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PKN22_09860"
     CDS             <1..1332
                     /locus_tag="PKN22_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43051.1"
                     /translation="PTYKLIGLLVMLFGAFLIVRPYINRPDPLPTIEDRLPDAEYMAT
                     VDCIRLAREVSGMMFYYKISYRDFLSPEFILTQAKSYGLDLQKTSYFFGNSNGEFGVL
                     ANLIDSTRLNQGIEKLKYFFETKEYKIQKQKVIKLVDHNAYLFYGNDYICYYQGNDIK
                     KQIKRICYAENSHISPNWVELINQKHYIDKCAIISSKLPDFDVLNIEHALAYPLFDST
                     GVTFNLQIKTKDTIPFKLKEGGPDFVQGTFTKMAMNLHLDPTYLKNHPEHPLYQYLLN
                     MRTRIRLPIKDFISNWGGDLSFHQGGWVNVKQNYIESELDDNFNITEVVRTKETKVPG
                     FAMYYSLAENSQRFKDLMLRKGFVTEQEGKFHFLLSPPLNFARTKNNEEIFYAASVLP
                     KTIRSVKSYVMWSSMRTKFTAKIDSISTFNFYGSLNFSLKNILQKEKLTDE"
     gene            complement(1388..1567)
                     /locus_tag="PKN22_09865"
     CDS             complement(1388..1567)
                     /locus_tag="PKN22_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43052.1"
                     /translation="MKVLNKYKKTFHTKVFDNLELANVKTVQTALAKKEKIKEQSLIR
                     AYESLNKLKKFGINI"
     gene            complement(1570..>2459)
                     /locus_tag="PKN22_09870"
     CDS             complement(1570..>2459)
                     /locus_tag="PKN22_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNS43053.1"
                     /translation="LALLFGVERPGITQHIKNIFSEGELQENSVSKKFLRTATDGKKY
                     NTKHYNLDMIISLGYRVNSKVATQFRIWATKRLNEYIRKGFTMDDERLKNLGGGGYWK
                     ELLQRIRDIRSSEKVFYRQVLDIYATSIDYDPKADVSIEFFKKVQNKIHFAVHGQTAA
                     ETIFNRADAEKEFMGLMTFSGSRPYLKDVVVAKNYLNEKELRSLGQIVSGYLDFAERQ
                     AEREQAMTMIDWAEHLDRILTMSGEKLLQGAGTVSHEKAVEKATAEYKKYQQKTLSEA
                     EKNYLESLKAIEKKTTNKR"
BASE COUNT          694 a          502 c          432 g          831 t
ORIGIN      
        1 cccacatata aactaatcgg attattagtt atgttatttg gtgcattttt aatagtgcgt
       61 ccctatatca atcgtcctga tccgctacca actattgaag atcgtttgcc cgacgccgaa
      121 tacatggcaa cggtagattg catccgattg gcgcgcgagg tgagcggcat gatgttttat
      181 tacaaaattt cgtaccgcga ttttctttct ccagagttta ttttaacgca agccaaatcg
      241 tatggtttgg atttgcaaaa aacctcctat ttcttcggca atagcaacgg cgaatttggt
      301 gttttggcca atttgattga tagcactcgc ctcaaccagg gaattgaaaa attaaagtat
      361 ttttttgaga ctaaagaata taaaattcaa aagcaaaaag tcattaagtt ggtcgaccac
      421 aatgcgtatt tgttttatgg caacgattac atttgctatt accaaggcaa cgacatcaaa
      481 aagcaaatta agcgcatttg ttatgccgaa aatagccata tttcgcccaa ctgggtagag
      541 ttgatcaacc aaaaacatta cattgacaaa tgtgccatta ttagttccaa attgcccgat
      601 tttgatgttt taaacattga gcatgcgttg gcttatccgt tgtttgactc aaccggcgtg
      661 acctttaatt tgcaaataaa aacaaaagac accattccgt ttaagctcaa agaaggcgga
      721 cccgattttg tgcaaggcac ctttaccaaa atggcgatga acctgcattt ggatccaacg
      781 tatttaaaaa accatcccga acatccgctt tatcaatact tgctcaatat gcgcacacgt
      841 attcgtttac ctatcaaaga ttttatttct aactgggggg gcgatttatc gtttcaccaa
      901 ggtggttggg tcaatgtgaa gcaaaattac attgaatcgg agttggacga taattttaac
      961 attaccgaag tggtgcgcac caaggaaaca aaggtgcctg gttttgctat gtattactcg
     1021 cttgccgaaa actcacaacg tttcaaagat ttgatgctgc gcaagggatt tgttaccgag
     1081 caagagggga aatttcactt tttgttgtcg ccaccattga actttgcacg cacaaaaaac
     1141 aacgaagaaa tattttatgc tgccagcgtg ttgcccaaaa ccattcgttc ggttaaatcc
     1201 tacgtaatgt ggtcgagtat gcgcaccaaa tttaccgcca aaatcgacag tatttctacc
     1261 ttcaattttt atggcagtct aaatttcagc ctcaaaaaca ttttgcaaaa agaaaagttg
     1321 acagatgagt gatggttagc cgactctaag cttcgctgca tctttccata aataatgagg
     1381 cattggttta tatatttatt ccaaattttt tcagtttatt taacgattca tacgcacgta
     1441 ttaatgattg ctctttaatt ttttcctttt ttgctaatgc ggtttgaacc gttttaacat
     1501 tggctaattc aaggttgtca aatacttttg tatgaaatgt tttcttgtat ttatttaaaa
     1561 ctttcactat tatctcttgt ttgttgtttt cttttctatc gctttcaaac tttccaaata
     1621 gtttttttcc gcttcactca atgttttttg ttgatacttt ttgtattctg ctgttgcttt
     1681 ttccactgcc ttttcgtgac tgaccgttcc tgctccctgc aacagtttct ctccgctcat
     1741 tgttaaaatc ctgtccaaat gttcagccca atctatcatt gtcattgctt gctctctttc
     1801 agcttgtcgc tcagcaaagt ctaaataccc cgaaacgatt tgtcctaatg aacgaagctc
     1861 tttttcgttc agatagttct ttgctacaac aacatctttc agatatggac gacttccaga
     1921 gaaggtcatc aaacccataa attctttttc agcatcggca cgattaaaaa tcgtttcggc
     1981 tgcggtttgt ccgtgaacag cgaaatgaat tttgttttgt acttttttaa aaaattcaat
     2041 agaaacatct gctttcgggt cgtaatcaat acttgtagcg taaatatcca gtacctgacg
     2101 gtaaaacact ttttcagaag aacgaatgtc tcgtatgcgt tgcaacagtt ctttccaata
     2161 accaccacct cccagatttt tcaaacgctc atcgtccatt gtaaagcctt tgcgaatgta
     2221 ctcattcagg cgtttggttg cccaaattct gaattgtgta gctacttttg aattgacacg
     2281 ataacccaat gaaataatca tatcaagatt atagtgcttg gtgttgtact ttttaccgtc
     2341 tgtggcagtt cgtaagaatt tcttactaac tgaattttct tgtaattctc cttctgaaaa
     2401 aatgtttttt atgtgctgtg ttataccagg acgctctact ccaaaaagca atgctaatt
//
LOCUS       DAOQVQ010000379         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_52251_length_2454_cov_3.292205, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000379 DAOQVQ010000000
VERSION     DAOQVQ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52251_length_2454_cov_3.292205"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..676
                     /locus_tag="PKN22_09875"
     CDS             143..676
                     /locus_tag="PKN22_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNS43054.1"
                     /translation="MQFQVGEKVVFLNEAGGGRITKIVGNLIYVEDETGFDRPFKAAD
                     LGKVHSTNYSVENKDKIVKDVVPAIEKKRAYTIFKDYWEVDLHLEFLVAIYGANLIKQ
                     PDQALLKQMSIFKDVLQQAKAKKVRKLIVIHGFGKGMLRQELQDYLRGQDGIDFFDAP
                     YVEYGHGAIQVEIKYKY"
     gene            792..1889
                     /gene="ychF"
                     /locus_tag="PKN22_09880"
     CDS             792..1889
                     /gene="ychF"
                     /locus_tag="PKN22_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNS43055.1"
                     /translation="MALKCGIVGLPNVGKSTLFNCLSNAKAQSANFPFCTIEPNLGTI
                     SVPDPRLEKLEAIVKPERVVPTTMEIVDIAGLVKGASKGEGLGNQFLANIRETDAIIH
                     VLRCFDNGNIVHVDGSVNPVRDKEIIDIELQLKDLETIEKKIQSLSRVIKSGDKDALK
                     ENALALQIKEGLEQGKSVRGLGFDAEEMEIVKKFQLITSKPVLYLCNVDEASVKTGNS
                     YVDAVKEAVKNEDAEVLVLGAQIVADINELESYEERQLFLDELGLEEPGVNRLIRSAY
                     ALLNLQTYFTAGVKEVRAWTFPIGATAPQAAGVIHSDFEKGFIRAEVMKYDDYVTLGS
                     EAAVKEAGKFKVEGKEYIVQDGDIMHFRFNV"
     gene            1921..>2454
                     /locus_tag="PKN22_09885"
     CDS             1921..>2454
                     /locus_tag="PKN22_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNS43056.1"
                     /translation="MSEILANNPAIKTWIEVPENSDFPIQNLPFGIFKTDSLSPRVGS
                     RIGNSIIDLKTLYALGYLSNLPFSEEDFDTNYLNPMMQKGKLATRNLRNRLSKLFSTD
                     NNDLRNNSHHVDQIIVAAENAQMLLPVQIGDYTDFYSSREHATNVGMMFRDPNNALLP
                     NWLWIPVGYHGRASSVIV"
BASE COUNT          816 a          399 c          488 g          751 t
ORIGIN      
        1 tcgcaagtta attcggtgtt aatcaccccc gtaaaacaag atgctaactt atttcaaaaa
       61 ttagcaatta ctcttttgtg gttgcttgct ttggccagtc cgtttattct ttatttttta
      121 atcgatttaa actgggtgca atatgcagtt tcaagttggt gagaaagtag tgtttttaaa
      181 tgaagctggc ggtggccgaa tcactaaaat tgtaggaaac cttatatatg tagaagatga
      241 aacagggttt gaccgtccat tcaaagccgc cgatttagga aaagttcaca gcaccaatta
      301 ttctgttgaa aacaaagata aaattgtaaa agatgttgtt cctgcaatag aaaaaaaacg
      361 tgcttacaca attttcaaag attactggga agtggattta cacttggaat ttttagtagc
      421 tatttacggt gccaatctta tcaaacaacc cgatcaagct ttgctcaaac aaatgtccat
      481 ttttaaagat gttttgcagc aagctaaagc aaaaaaagta agaaagctta tcgttattca
      541 cggttttggc aaaggaatgt tacgccaaga acttcaagac tacctgcgcg ggcaagatgg
      601 cattgatttt tttgacgcgc catatgtaga atacggtcac ggtgctattc aagttgaaat
      661 aaaatacaag tattagttct ctttttactg aatcaaccaa ccaaagaagt actttcaaac
      721 atcaattctt tttcttaact ttgcaaactt ttagaagtaa aaaattgaat ttataacatc
      781 aaacaattat aatggcatta aaatgtggta ttgttggttt accaaatgta ggtaaatcta
      841 ctcttttcaa ctgtttatca aacgcaaaag cgcaatcggc aaactttcca ttttgtacga
      901 ttgaacccaa tttaggaaca atttcagttc cagatcctcg cttagaaaaa ttagaagcga
      961 ttgtcaaacc tgagcgtgtt gttccaacca ctatggaaat tgtggatatt gctggtttgg
     1021 taaaaggtgc atcaaaaggt gaaggattag gtaaccaatt tttggcaaac attcgtgaga
     1081 cggatgctat cattcatgta ttgcgttgtt ttgataatgg aaacattgta cacgttgacg
     1141 gatcggtgaa cccagttaga gacaaagaaa ttatcgacat cgaattgcaa ttaaaagact
     1201 tggaaacgat tgaaaagaaa attcaatcgc tttcacgtgt tattaaatct ggtgataaag
     1261 atgcgttaaa agaaaatgct ttggctttgc aaataaaaga aggtttggag caaggaaaat
     1321 cggtgcgtgg tctaggtttt gacgccgaag aaatggaaat cgttaaaaaa ttccagttga
     1381 ttacttcaaa accagtgttg tatttatgca atgtggacga agcgtctgta aaaacaggaa
     1441 acagttatgt tgatgcagtg aaagaagcag taaaaaatga agatgcagaa gttttagtat
     1501 tgggcgctca aatagtagcc gacatcaacg agttggaatc ctacgaagaa cgtcaactat
     1561 ttttggatga attaggcttg gaagaacctg gtgttaaccg tttgattcgc tcagcttacg
     1621 ctttattgaa cttgcaaact tatttcacag caggtgtaaa agaagtaaga gcttggacgt
     1681 ttccaatcgg tgcaacggct ccacaagcgg ctggtgttat ccactctgat tttgaaaaag
     1741 gatttatccg tgccgaagtg atgaaatacg acgattacgt tactttaggt tcagaagctg
     1801 ctgtgaaaga agctggtaaa tttaaagtag aaggaaaaga atacattgtt caagatggtg
     1861 atatcatgca tttccgtttc aatgtttaat gcagtaattt aaaataaaaa gtaaaaaact
     1921 atgtcagaaa tactagcaaa taatccagcc ataaaaacat ggattgaagt tccagaaaac
     1981 agtgattttc cgattcaaaa tttgcctttt ggtattttta aaacggattc actttctcca
     2041 cgtgttggtt cgcgtattgg taactctatc attgacttaa aaacgcttta tgcattgggt
     2101 tatcttagca atttaccttt ttcggaagaa gattttgata ccaattattt gaatccaatg
     2161 atgcaaaaag ggaaattagc aacacgtaac ttgagaaatc gcttgtctaa attattttct
     2221 acggataata atgatttaag aaacaactca caccacgttg atcaaatcat cgttgcagca
     2281 gaaaatgctc aaatgctttt gcccgttcaa attggagatt acaccgattt ttactcttcg
     2341 cgcgagcatg caaccaacgt tggaatgatg tttagagatc ctaataatgc tttattgccc
     2401 aactggttgt ggattcctgt aggttaccac ggaagagcta gttctgttat agtt
//
LOCUS       DAOQVQ010000380         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_52265_length_2454_cov_1.932055, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000380 DAOQVQ010000000
VERSION     DAOQVQ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52265_length_2454_cov_1.932055"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKN22_09890"
     CDS             complement(<1..323)
                     /locus_tag="PKN22_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNS43057.1"
                     /translation="MEFTKKSIGHIAIFTSSLIFGVNLPLSKTILPLYVDAQMLTLFR
                     VCGAALLFWVLSLFVKKESVPFKDLLLLFGASMFGIVINQFAFLEGLSRSSVIDTGIL
                     LTITP"
     gene            437..1336
                     /locus_tag="PKN22_09895"
     CDS             437..1336
                     /locus_tag="PKN22_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS43058.1"
                     /translation="MAFLEVKNVRKQYANHLALDNVSLEIEKQHVYGLLGPNGAGKTT
                     LIRIINNITAPDSGEVLLNGKPLNANDTARIGYLPEERGLYKKMKVGEQALYLCQLKG
                     LSKPDALKRLKYWFEKFEIENWWDKKVEELSKGMAQKVQFITTVLHQPELLIFDEPFS
                     GFDPVNADLLKREILELRDNGATIIFSTHNMESVEAICDDISLINHSRVVLQGSVSDI
                     QQQFKENIYSALIRTQELKNSDLFDIIDKKVMGNQTQVRLKTALESNALLQALISDYE
                     VKAFNEELPSMHEIFVKTVTENH"
     gene            1343..>2454
                     /locus_tag="PKN22_09900"
     CDS             1343..>2454
                     /locus_tag="PKN22_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS43059.1"
                     /translation="MKTDQLHFETLQVHAGFTPDGNNPATPPIHPSNAFTFDTAEHGA
                     DLFNLDFKAGKSPFIYTRLNNPTNSIFEQRMAALEGGVAAVAVASGQAAQMITIMNIM
                     KSGDNFVTSPYLYGGTYNQFFVSFKDLGIEARKAASDKAEDMEKLIDANTKALYTENI
                     GNPYFNVPDFEKLARLAQKYEIPLIVDNTFGCAGYLCQPIKHGANIVVESATKWIGGH
                     ATVMGGVIVDGGNFNWGNGKFPKYTEPSEGYHGLVFWEKFGNMSFAMRCVAENLRDMG
                     ACISPFNSWQMLQGIETLSIRVEKMCQQALTMAQWLETLPEVSSVSYLGLKKHPYHTL
                     ANQYLHNGYGAVLTFRVKGGLEATTRFVEKLKLIKHL"
BASE COUNT          844 a          471 c          473 g          666 t
ORIGIN      
        1 attggcgtga tggtcaaaag gattcctgta tcgataaccg aagaacgaga caagccttcg
       61 agaaaggcaa attgattgat aacaatacca aacatggacg ctccaaacaa caagagtaaa
      121 tctttaaatg gaactgattc tttcttcaca aacaacgaaa gtacccaaaa taacaaagcc
      181 gcaccgcaca cacgaaataa tgtcaacatt tgggcatcaa catagagtgg taatatggtt
      241 ttagagagtg gtaaatttac cccaaagatt aaactcgaag taaaaatggc tatatgacca
      301 attgattttt tagtaaattc cattaatttt caatataaac tcataattga acctaaactg
      361 cttggcaaag gtacatcttt tttcgttatc tttgcagcgt tttattctca taatcaaact
      421 aaaagaagaa caacttatgg cttttttaga agtaaaaaat gttagaaaac aatacgccaa
      481 ccatttggcg ctcgacaacg tatcgttgga aattgaaaag caacatgtct atggattatt
      541 aggtcccaat ggcgctggca aaactacact tattcgcata atcaataaca tcacggcacc
      601 tgatagcgga gaggttttac tcaatggtaa accacttaat gcaaatgaca cagcacgtat
      661 tggctatttg cccgaagaac gtggtttata taaaaagatg aaagtgggcg aacaagcgct
      721 ttatttgtgc caactcaaag gtttatcaaa acctgacgcg ttgaaacgtc ttaaatattg
      781 gttcgaaaaa tttgaaatcg aaaattggtg ggataaaaaa gtggaagaat tatcgaaagg
      841 aatggcacaa aaagtacaat tcattaccac cgtgctacat caaccagaac tactcatttt
      901 cgatgaacca tttagcggtt tcgaccctgt gaatgccgac ttattgaaac gagaaatttt
      961 ggaattacgc gataatggtg ccactattat attttccact cacaatatgg aatcagtaga
     1021 agctatttgc gacgatattt cgcttatcaa ccattcgcgc gttgtgcttc aaggaagcgt
     1081 tagcgacatt caacaacaat tcaaagaaaa tatttacagt gctttaatcc gcacacaaga
     1141 gctgaaaaac agcgatttat tcgatattat tgataaaaaa gtaatgggca atcaaacgca
     1201 ggtgcgatta aaaacagccc ttgaatcaaa tgcgctgcta caagcgctca tcagtgacta
     1261 tgaagtaaaa gccttcaacg aagaattacc aagtatgcac gaaatttttg tcaaaactgt
     1321 cacagaaaac cattaagatg ttatgaaaac agatcaatta cattttgaaa ccttgcaagt
     1381 gcacgcaggt tttacacccg atggcaataa tccagcaacg cctcctatcc acccaagtaa
     1441 tgcattcact tttgatactg ccgagcatgg agccgacttg tttaatttag atttcaaagc
     1501 aggaaaatcg ccctttattt acacgcgtct caataatccg accaatagca ttttcgaaca
     1561 acgtatggcg gcattagaag gtggcgtggc ggccgtggct gttgcttcag gacaagctgc
     1621 acaaatgatt accatcatga acatcatgaa aagcggtgat aattttgtaa catctcccta
     1681 tttgtatggt ggcacttaca accaattttt tgtgtcattc aaagatttag gtattgaagc
     1741 gcgcaaagct gcgagcgaca aagccgaaga tatggaaaaa ttaattgatg ccaatacaaa
     1801 agcactttat acagaaaata ttggcaatcc ttattttaat gttccagatt ttgaaaaatt
     1861 agcacgttta gctcaaaaat acgaaattcc acttattgtg gataacacgt ttggctgtgc
     1921 tggatatttg tgccaaccaa tcaaacatgg cgccaatatt gtagtagaat cggccacaaa
     1981 atggattgga ggacatgcca ctgttatggg cggtgtcatt gtagatggcg ggaatttcaa
     2041 ttggggtaat ggcaaattcc caaaatatac agaaccttca gaaggttatc atggattagt
     2101 tttctgggaa aaatttggga acatgtcgtt tgctatgcgt tgtgttgccg aaaatttacg
     2161 ggatatggga gcttgcatca gtccatttaa ctcttggcag atgttacaag gcattgaaac
     2221 actctccatt cgcgtagaaa aaatgtgtca acaagcttta actatggctc aatggttaga
     2281 aacactgcca gaagtgagct ctgtgagtta tttgggatta aaaaaacatc cttatcacac
     2341 actagccaac caatatttac acaatggcta cggcgcagta cttactttcc gagtaaaagg
     2401 tggactcgaa gctaccacac gttttgtaga aaaactaaaa ctcataaaac acct
//
LOCUS       DAOQVQ010000381         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_52288_length_2453_cov_2.806088, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000381 DAOQVQ010000000
VERSION     DAOQVQ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52288_length_2453_cov_2.806088"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PKN22_09905"
     CDS             <1..889
                     /locus_tag="PKN22_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43060.1"
                     /translation="ATSMCYYIKGLCAATNRDYEDSYLYFNKTRAVLDKNPIIKADLA
                     TRYIQCLTHLLRCYIDGQEFKQAEELIVDMRNLKEEKGFKSVSHQIRILSNSYLMEFL
                     LLQRQGKFQDTIRLIDNIGDFVIEYEDTLTKEQRIMFSYYKSVSYFAVGEYKKALLCV
                     NEILNDNEKIVRQDIFSFARIFNLVLHFELGNYDFAEYAIKSVNRYLSKRERDYETET
                     LLIKTIKQLSKTNTPEEQYTIFKSLKENVEELFNDKHEQAILEYFNLTAWIEAKLKST
                     TYEYEVKRLLKSNSAGNSN"
     gene            complement(857..2341)
                     /locus_tag="PKN22_09910"
     CDS             complement(857..2341)
                     /locus_tag="PKN22_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43061.1"
                     /translation="MHKHPKSVSTLFILLIATFSSFVNAQNDADLFIYSKEYYEGSAR
                     FNAMSGAFGSVGADISAAQINPAAMGRFSSSQVSISLGSTINNTTADFMSTKSKDLKS
                     SFSIPSIGLVFTKDLSNKNKGDMYSQFGIGMNRLINFRQNTTIEGQQFPSLLDNFIGQ
                     AQGYYPENLSAAFPFSTSLAWESYVINFDENNVGYTSYLNSGDMQMKRTIETTGGVNE
                     FFLSYSRNRLNKLYYGASLGLRTYSRTDAYVHSEDLVQKDTFFVGFDYKYQLKTSGYG
                     ANLKLGVIYLVTDGFRIGASIHTPTIISLTDKWTATMTGRFNGGQNVSVPQDLVPEGQ
                     YKYRMITPFKANVSASYVIGMSALISADIEYLGYNQARLKSTRDITYQAYDFKIENAD
                     AKKRLTNAVNFRLGAEYNIQQKLFIRAGMAIYGSAYKKSENVDPQPDLSFSGGLGYKF
                     GRFSIDLAYVNRAMKRVYYPFTGSNTANTKLSYNSITVSGTIRF"
BASE COUNT          810 a          433 c          444 g          766 t
ORIGIN      
        1 tgccacttct atgtgctatt acatcaaagg attgtgtgct gcaacaaaca gagattatga
       61 agacagctac ttgtatttca ataaaacgcg cgctgttttg gataaaaatc caattattaa
      121 agcagatttg gcaacacgct acattcaatg cttaacccac ttgttgcgtt gttatattga
      181 cggacaagag tttaaacaag cagaagagtt gattgtggat atgcgcaatt tgaaagaaga
      241 aaaaggattt aagtcggtta gtcatcaaat tcgcatttta tccaattcct atttgatgga
      301 atttttattg cttcaacgtc aagggaaatt tcaagatacg attcgcctaa tcgataatat
      361 aggggatttt gtaatagaat atgaagatac actcacaaaa gaacaacgca ttatgtttag
      421 ttactacaaa tcggtgagtt attttgctgt tggagaatat aaaaaagcgc ttttatgtgt
      481 caacgaaatt ctcaacgata acgagaaaat tgtgcgccaa gatattttta gtttcgcacg
      541 tattttcaac ttggttttgc actttgaatt aggaaattat gattttgccg aatatgccat
      601 caaatcagta aaccgttatc tatcaaaacg tgaacgtgat tacgaaacag aaacattgtt
      661 gattaaaacc atcaaacagc tttcaaaaac aaacacaccc gaagaacaat acaccatttt
      721 taaatcgctc aaagaaaatg tggaagaatt gttcaacgac aaacacgaac aagcaatttt
      781 ggagtacttt aatttaacgg cttggattga agcaaaactt aaaagcacta cttacgaata
      841 cgaagttaaa cgattgctaa aatcgaatag tgccggaaac agtaattgaa ttgtaggata
      901 attttgtatt agctgtatta cttcctgtaa acggataata aacacgtttc attgctcggt
      961 ttacataagc caaatctatg ctaaaacgtc caaatttata acccaatcct ccgctaaagg
     1021 ataaatctgg ttggggatca acgttttcag attttttata tgcgctgcca taaatagcca
     1081 ttcctgcacg tataaaaagt ttttgttgaa tattgtattc agcacccagt ctaaaattga
     1141 cagcattggt caatcgtttt ttagcatccg cattttcaat tttaaaatca tacgcttgat
     1201 aagtgatatc acgtgttgat tttaaacgtg cttggttgta tcccaaatat tcaatatcgg
     1261 cactaattaa tgcactcatc ccaattacat acgatgccga aacgttagct ttgaatggtg
     1321 taatcatgcg gtatttgtat tggccttctg gcactaaatc ttgtggaaca ctaacatttt
     1381 gccctccatt aaaacgtccg gtcatggtag ccgtccattt atcggtaagt gaaattattg
     1441 taggtgtatg aatagacgct ccaattctaa atccatcggt gacaaggtaa attacaccca
     1501 atttcaaatt agcaccatat ccactggttt ttagctggta tttataatca aatccaacaa
     1561 aaaatgtgtc cttttgaact aaatcttctg aatgaacata agcgtcagta cggctataag
     1621 tgcgcaaacc caatgaagca ccatagtaca acttgtttag acgattgcga gagtaagata
     1681 gaaaaaactc attaacacct ccagtggttt caattgttcg tttcatctgc atatccccac
     1741 tgttgaggta agacgtataa cccacattgt tttcatcaaa attaatcaca tacgattccc
     1801 aagctaatga tgtggaaaaa ggaaatgcgg cacttagatt ttcaggataa taaccttgag
     1861 cttgccctat gaaattatct aatagtgaag gaaattgctg tccctcaatg gtggtgtttt
     1921 gcctaaaatt aatgagtcga ttcatgccaa ttccaaactg agaatacatg tctcctttgt
     1981 ttttattgga taaatctttt gtaaaaacaa gtccaattga aggaatggaa aagcttgatt
     2041 ttaagtcttt tgatttggtt gacataaaat cagccgttgt gttgttgatt gtagaaccca
     2101 aggaaatgct aacttgcgat gatgaaaaac gccccatggc agctggatta atttgtgctg
     2161 cgctaatatc agctccaacc gaaccaaatg caccactcat ggcattaaaa cgagcacttc
     2221 cttcatagta ttcttttgag taaatgaata aatcagcatc gttttgtgcg ttaacaaaag
     2281 aactaaaagt tgctattaat agaataaata aagttgaaac tgactttggg tgtttgtgca
     2341 tataatttat cttcttcctg agttggtatt gttataactt ccgcttgatg aacggctact
     2401 tggtgtggat gatggtgtgc taaaacgttc tggtcgcgct gaagaattaa acg
//
LOCUS       DAOQVQ010000382         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_52413_length_2449_cov_3.345865, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000382 DAOQVQ010000000
VERSION     DAOQVQ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52413_length_2449_cov_3.345865"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="map"
                     /locus_tag="PKN22_09915"
     CDS             <1..506
                     /gene="map"
                     /locus_tag="PKN22_09915"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNS43062.1"
                     /translation="ISVDCGVRLNGFCGDSAYTFGVGEINSNVKHLLKVTKQALLEGI
                     DNAVVGKRIGDIGFAIQNLCENSGYGVVREFVGHGIGRNMHEDPQVPNYGRKGYGPLL
                     KTGMCIAIEPMITLGSRLIIMERDGWTVRTKDCSYSAHFEHTVAITNKEADVLSTFDY
                     IDEVLRI"
     gene            521..739
                     /gene="infA"
                     /locus_tag="PKN22_09920"
     CDS             521..739
                     /gene="infA"
                     /locus_tag="PKN22_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNS43063.1"
                     /translation="MAKQSAIEQDGIIVEALSNAMFRVELDNGHEITAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLSKGRIVFRYK"
     gene            748..864
                     /gene="ykgO"
                     /locus_tag="PKN22_09925"
     CDS             748..864
                     /gene="ykgO"
                     /locus_tag="PKN22_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNS43064.1"
                     /translation="MKVRASLKKRTSECKIVRRNGRLYVINKKNPKFKQRQG"
     gene            898..1278
                     /gene="rpsM"
                     /locus_tag="PKN22_09930"
     CDS             898..1278
                     /gene="rpsM"
                     /locus_tag="PKN22_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNS43065.1"
                     /translation="MAIRIVGVDLPQNKRGEIALTYVYGIGRSSSAKILEKAGVDKNL
                     KVKDWSDDQAASIREIIGAEFKVEGDLRSEVQLNIKRLMDIGCYRGVRHRIGLPVRGQ
                     STKNNARTRKGRKKTVANKKKATK"
     gene            1290..1679
                     /gene="rpsK"
                     /locus_tag="PKN22_09935"
     CDS             1290..1679
                     /gene="rpsK"
                     /locus_tag="PKN22_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNS43066.1"
                     /translation="MAKKTVAAKKRNVKVDANGQLHVHSSFNNIIVSLANSEGQIISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKIAFDLGLRKVKAYVKGPGNGRESAIRTIHG
                     AGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            1787..2392
                     /gene="rpsD"
                     /locus_tag="PKN22_09940"
     CDS             1787..2392
                     /gene="rpsD"
                     /locus_tag="PKN22_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNS43067.1"
                     /translation="MARYTGPKSRIARKFGEGIFGADKVLSKKNYPPGQHGNSRKRKT
                     SEYGIQLREKQKAKYTYGVLEKQFRNLFEKAETAKGITGEILLQLLEGRLDNVVFRLG
                     IAPSRAAARQLVSHKHITVDGSVVNIPSYAVKPGQVIGVRERSKSLEVISNSLAGFNH
                     SKYPWLEWDDNAKVGKLLHIPERTDIPENIKEHLIVELYSK"
BASE COUNT          820 a          328 c          532 g          769 t
ORIGIN      
        1 ttatttctgt tgattgtgga gttcgcctca atggtttctg tggcgattct gcatatacgt
       61 ttggtgtggg tgaaattaac agcaatgtga aacatttgct gaaagttaca aaacaagcgt
      121 tgctagaagg tatagataat gctgtcgtag gtaaacgcat tggtgatatt ggtttcgcaa
      181 tacagaattt gtgtgaaaat agtgggtacg gagttgttcg tgagtttgta ggtcatggta
      241 ttggtcgaaa tatgcacgaa gatcctcagg ttccaaatta tgggagaaaa ggttatggac
      301 ctttactaaa aacaggaatg tgcatcgcaa ttgaacccat gataacttta ggcagtagac
      361 ttattattat ggagcgtgat ggttggactg tgagaaccaa agattgttcc tattctgcac
      421 attttgaaca tactgtagct ataacgaaca aagaagctga tgttttatct acttttgatt
      481 acattgatga ggttttaaga atttgagtat tttaatattt atggcaaagc aatctgcaat
      541 agaacaagat ggaataatag ttgaggcgtt gtctaatgca atgtttcgag ttgagttaga
      601 taatggacat gagattacgg ctcatatttc aggtaagatg agaatgcatt atataaagat
      661 tttacctgga gataaagtta gagttgaaat gtctccctat gatttatcca aaggaagaat
      721 agtgtttaga tataaataaa tttagatatg aaagtaagag catcattaaa aaaacgcacg
      781 tcagaatgta aaattgttag acgtaacggt cgtttgtatg ttattaacaa gaaaaatcct
      841 aagtttaaac aacgtcaagg ataatttctt atttttgcaa gataaatatt ttattttatg
      901 gctataagaa tagttggtgt agatttacct caaaataaga gaggagaaat agcgttaacc
      961 tatgtatatg gtataggacg cagtagttca gctaaaattc tagagaaagc tggtgtagat
     1021 aaaaacctta aagtcaaaga ttggtcagat gatcaagcgg catcaattcg tgagattatt
     1081 ggtgcagaat ttaaggttga aggtgatctt cgttctgaag ttcaattaaa tattaaaaga
     1141 ttaatggaca ttggttgcta tcgtggtgtc cgtcatcgta ttggcttacc tgttcgtggt
     1201 caaagtacta agaataatgc acgaactcgt aaagggagaa agaaaaccgt tgctaataag
     1261 aaaaaagcta ctaaataata attcttgata tggcaaaaaa aactgttgca gcaaaaaaaa
     1321 ggaatgttaa agttgatgct aatggacaat tacatgttca ttcctcattc aataacataa
     1381 ttgtttcact tgctaatagt gaagggcaga taatttcgtg gtcttctgct ggaaaaatgg
     1441 gctttagagg ctctaaaaag aatacccctt acgcagctca aatggctgca caagattgtg
     1501 caaaaattgc ttttgatctt ggacttagaa aagttaaggc ttatgttaaa ggacccggta
     1561 atggtagaga gtcagcaatt cgtactattc atggtgctgg tattgaggtt acagagattg
     1621 tcgatgtaac tccattgcca cataatggat gtcgtcctcc taaaagacgt agagtttaat
     1681 taataaaatg agacttaatt ttgtttatgg attgcactct ttcttctctg taatcgcggc
     1741 tgcaacaaat taagttgatg attactataa tttaataaaa ataataatgg ctagatatac
     1801 aggaccaaaa tctagaattg cgcgtaaatt tggtgaaggc atttttggag ccgataaggt
     1861 gttgtcaaag aagaactatc ctccaggaca acatggtaat tctagaaaaa gaaaaacttc
     1921 tgaatacgga atccaacttc gtgagaagca aaaggcaaaa tatacttatg gtgttttgga
     1981 aaaacaattt cgtaatttgt ttgaaaaggc tgagaccgct aaaggtatta caggtgagat
     2041 tttgcttcaa ttactagaag ggcgtttgga taatgtagtg ttccgtcttg gaattgctcc
     2101 atcgcgtgcc gcagctcgtc agttagtaag tcataaacac ataactgttg acggtagtgt
     2161 tgttaatatt ccttcatatg cggtgaaacc tgggcaagtt attggtgtaa gagaacgttc
     2221 aaagtcttta gaagtaatta gtaattctct tgcaggcttt aatcatagta aatatccatg
     2281 gttagaatgg gatgacaatg ctaaagtagg taaattgctt catattcctg agcgtacaga
     2341 tattcctgag aatattaaag agcatttgat tgttgagttg tattctaaat aataattagt
     2401 tccatggcga tattagcatt tcaaaaacct gataaagtat taatgttgg
//
LOCUS       DAOQVQ010000383         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_53056_length_2430_cov_4.437053, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000383 DAOQVQ010000000
VERSION     DAOQVQ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53056_length_2430_cov_4.437053"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2430
                     /locus_tag="PKN22_09945"
     CDS             <1..>2430
                     /locus_tag="PKN22_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43068.1"
                     /translation="EQQIALQKQEELAKAEQAKQTANETNVTTEQPAPTAQQVIEKWQ
                     AAYNGNLKNDLASSPQSVEETTQTIDRLAAYRAQINQPSNEAVNNANYKSALEQENKR
                     IDARINELKQQKTSLEQQIALQKQEELAKAEQAKQTTNETNASNNELSQNNEVVNNQQ
                     KVENKTTNTNEQAQPTVEKVVKTPAIIVGELQKTYNGDIQADFESQSNSIDAIEENIV
                     RLTNYRAQINHSTAKKEDKAAHQQAVEQENKRIDNRIQQLEAQIEDLQQQQIAFEKPE
                     EVENVAEQKTIEQTSTNIDTAKTIVENPTNNEVENNNLSSIEKQDTIPTQELAIEKVE
                     ETQPTLSKIDSLTAQLNATELSKKERKAVQQQIATIQKEEAENRIVAINQSNQIYQQQ
                     IDALSETGNKNAVQLQYEQKQAALKNQLAKEKSVVGQELLLRQLLEQNQTHLAYLEQA
                     NELKENQQIAENNEGSRIYSEETLQDKKRRFTIEIGELENLRSEKQNALSAAKKSKEK
                     AQLATEISAIDTQQQLLRQELAFIDAQLSLNQPLEKPIDLAHNNVVLTYNEERQIASS
                     ENYEHYAIKIAQQNQLVEQHAVKSEQLTQAQQQLAHIREQEISDTRSEKTTLEQEKRA
                     IIDTIANISLSLSELETKIAHINNEANSYLPTETTEAMKFQNLVARGINPIKKSLVAT
                     ALIPISANGIDYNPTTPLVATTKAIPVDVLSPKGLVYRVQVGAFAKALPEGHFKEFTP
                     VTGEKLENSNIVRYMAGYFNNANSVVEAREKIRTFGYNDAFVVAYCDGKRIPFSEARK
                     LEESGQCVGNN"
BASE COUNT         1013 a          472 c          404 g          541 t
ORIGIN      
        1 tggaacaaca aattgcactg caaaaacaag aagaattagc taaagcagaa caagctaaac
       61 aaacagcaaa cgaaaccaat gtaacaacag aacagccagc gccaactgct caacaggtaa
      121 ttgaaaaatg gcaagcagcc tacaatggaa atctaaaaaa tgatttagct tcatcgcctc
      181 aatcggtgga agaaaccacg caaacaattg accgtttagc ggcttatcgt gcacaaatca
      241 atcaaccttc caatgaagct gtcaataatg ccaattacaa aagcgcattg gaacaagaaa
      301 ataaacgcat tgatgcacgt atcaacgagt tgaaacaaca aaaaacttcg ttggaacaac
      361 aaattgcact gcaaaaacaa gaagaattag ctaaagcaga acaagccaaa caaacgacca
      421 atgaaaccaa tgcatcaaac aatgaactta gtcaaaacaa tgaagttgta aataaccaac
      481 aaaaagtaga aaacaaaaca accaatacaa atgagcaagc acaaccaaca gttgaaaaag
      541 tcgtaaaaac tccagctatt attgttggtg aattacaaaa aacatataat ggtgacatcc
      601 aagctgattt tgaatcgcaa tccaattcaa ttgatgctat tgaagaaaac attgttcgtt
      661 taaccaatta tcgtgctcaa atcaaccata gcactgccaa aaaagaagac aaagcggcac
      721 atcaacaagc cgttgaacaa gagaataaac gcattgacaa ccgaattcaa cagttagaag
      781 cacaaattga agatttgcaa cagcaacaaa ttgcttttga gaaaccagaa gaagtagaaa
      841 atgtagctga acagaaaacg attgaacaaa catctacaaa catcgataca gcaaaaacta
      901 ttgtagaaaa ccctacaaat aatgaagttg aaaacaataa tctttcatcc attgaaaaac
      961 aagatactat tccaacgcaa gaattagcaa ttgaaaaagt agaggaaact caaccaactc
     1021 tttcaaaaat tgacagttta acagcgcaat tgaatgcaac tgaattgagt aaaaaagaac
     1081 gaaaagccgt tcaacaacaa attgcaacta ttcaaaaaga agaggctgaa aaccgcattg
     1141 tagcaatcaa tcaaagcaat caaatttatc agcaacaaat tgatgcctta tctgaaacag
     1201 gaaacaaaaa tgcagttcaa ttgcaatacg aacaaaaaca agcagccctt aaaaatcaat
     1261 tagcaaaaga aaaatcagtt gttgggcaag aactgctctt acgccaattg ttagagcaaa
     1321 accaaacgca tttagcatat ttggagcaag ccaacgaatt gaaagaaaac caacaaattg
     1381 ctgaaaacaa cgaaggttca agaatctatt ctgaagaaac attgcaggat aaaaaacgtc
     1441 gatttacaat tgaaatcggt gagttagaaa acctaagaag tgaaaaacaa aatgctttgt
     1501 ctgctgccaa aaaatcaaaa gaaaaagcac agctagcaac agaaattagc gccattgata
     1561 cacaacaaca attgttacgt caagaattag catttattga tgcacaattg agtttaaacc
     1621 aaccgttaga aaaacccatt gatttggctc ataacaacgt tgttttaacc tataatgaag
     1681 aacgacaaat tgcatcaagt gaaaattatg agcactatgc catcaaaata gctcaacaaa
     1741 atcagttggt agaacaacat gccgtaaaga gtgaacaatt aacacaagct caacaacaac
     1801 ttgcacatat tcgtgaacaa gaaatttcgg atactcgctc agaaaaaaca actttggaac
     1861 aagaaaaacg tgctataatt gacactattg ccaatatctc cttatctttg agtgaactcg
     1921 aaactaaaat tgctcacatc aataacgaag ctaatagtta tttacccact gaaactacag
     1981 aagctatgaa atttcagaat ttagttgcac gcggcatcaa tccaatcaaa aaatcattgg
     2041 ttgccactgc tttgattcca atttctgcca acggaattga ttacaacccc actactccac
     2101 tggtagcaac taccaaagcc attccagttg atgttctttc accgaaaggt ttggtttacc
     2161 gtgttcaagt aggtgcattt gcaaaagcgt tgccagaggg acatttcaaa gaatttacac
     2221 ctgtgaccgg tgaaaaattg gagaattcca acattgtgcg ttacatggct ggttatttca
     2281 acaatgctaa ttctgtggta gaagcacgcg aaaaaattag aacatttggt tacaacgatg
     2341 cttttgtagt ggcgtattgc gatggaaaac gcatcccatt ttctgaagct cgaaaattag
     2401 aagaatcagg acaatgtgtg ggcaataatc
//
LOCUS       DAOQVQ010000384         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_53115_length_2428_cov_4.111673, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000384 DAOQVQ010000000
VERSION     DAOQVQ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53115_length_2428_cov_4.111673"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKN22_09950"
     CDS             complement(<1..557)
                     /locus_tag="PKN22_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS43069.1"
                     /translation="MKYKFYLIQFLVVLFVNTNTLAQDFQSYLKANTIEKKCEIADFI
                     AVDFEFNNLDSLRMLAQDLLRCSYQEKSEKGKNLAFFILGKYFIRVAKEREGIEFINK
                     SKNYYFKNDDYNKITEIYNEIGNAYQYLGQHEEACKWYDFSLKYGKKASDDYISNIAS
                     VNLAQAYSKLGNYELAKQHAEQYKD"
     gene            626..2254
                     /gene="pruA"
                     /locus_tag="PKN22_09955"
     CDS             626..2254
                     /gene="pruA"
                     /locus_tag="PKN22_09955"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNS43070.1"
                     /translation="MANAFFNVPTPVNEPVRAYIPGSKEHTDLINKYKELYTQAEIEV
                     PMYIGGKEVKSDDKRPMTPPHDHQKVLGYYYQGNAKNVEQAIEAAMNAREAWANTPWE
                     QRAAVFLRAAELLAGPFRDKMNAATMLAQSKNVFQAEIDAACEMIDFFRFNVQYMTDI
                     YRDQPISSPGVWNRMEYRPLEGFVFALSPFNFTSICANLSASPAMMGNVVVWKPAASQ
                     IYSAAVIMELFKAAGLPDGVINMVAVDGATAGEVIFKHKDFAGIHFTGSTGVFRNLWK
                     TIGENLDKYKSYPRIVGETGGKDFVVAHKSANAAEVATALSRGAFEYQGQKCSAASRA
                     YIPSNLWNEVKEILVSQVSSMKMGSPEDTSNFINAVIDKKAFGKITEYIEYIKNASDA
                     EIVVGGNYDGSKGYFIEPTVCVTTNPKFKTMEEEIFGPVLTIYVYDENKYEETLKLVD
                     ETSEYALTGSIMATDRYAIDLAVNMLRNAAGNFYINDKPTGAVVGQQPFGGARGSGTN
                     DKAGSAMNLMRWVSLRTIKETFVTPKDYKYPFLG"
BASE COUNT          775 a          414 c          462 g          777 t
ORIGIN      
        1 cagtctttgt attgctctgc atgttgcttt gctaattcgt aattaccgag tttactatat
       61 gcttgagcta agttgacact tgcaatgttg ctaatgtaat cgtcacttgc ttttttgcca
      121 tatttaagtg aaaaatcata ccatttacac gcctcttcgt gttgacctaa atattggtat
      181 gcatttccaa tttcgttata gatttctgtt attttattgt aatcatcgtt tttaaaataa
      241 taatttttcg atttattgat gaactcaatc ccctctcttt ctttggctac gcgtataaaa
      301 tatttaccca aaataaaaaa agctaaattt tttccttttt cacttttttc ttgataggaa
      361 caccttaata ggtcttgcgc taacatgcga agagaatcta agttattaaa ttcaaaatca
      421 acggcaatga aatctgcaat ctcacatttc ttttcaattg tatttgcttt taaatacgat
      481 tgaaaatctt gtgctaatgt gttagtgtta acaaatagta caactaagaa ttgtattaga
      541 taaaatttat acttcaaatt tgttagctca ttcattttat aattgcaatt atatggaaca
      601 ttcactattt ttgcaagaaa aaattatggc aaatgcattt ttcaatgttc ctacacctgt
      661 caacgagccg gtgagagcat atatacctgg ttcaaaagaa cataccgact taattaacaa
      721 atacaaagaa ctttatactc aagctgaaat tgaagttcct atgtatatcg gaggaaaaga
      781 agtaaaatcg gatgataaac gtccaatgac tcctcctcat gatcaccaaa aagtattggg
      841 ttattattac caaggaaacg ccaaaaatgt agagcaagca attgaagcgg ctatgaatgc
      901 tcgtgaagct tgggcaaata caccttggga acaacgtgca gctgtgtttt taagagccgc
      961 tgaattattg gctggaccat tccgcgataa aatgaatgca gctactatgt tggcacaatc
     1021 aaaaaatgta tttcaagcag aaattgatgc tgcctgcgaa atgattgatt tcttccgttt
     1081 caatgtgcaa tacatgacag atatttacag agatcaacca atttcttcgc caggagtttg
     1141 gaacagaatg gaataccgtc cgttggaagg atttgtattt gctttgtctc cgttcaactt
     1201 tacatctatt tgtgctaatt taagtgcatc tcctgcaatg atggggaacg tagttgtttg
     1261 gaagcctgct gcatcgcaaa tttattcggc tgccgtgatt atggaattgt tcaaagctgc
     1321 tggtttacca gatggtgtta tcaatatggt ggcagtggat ggcgcaacag ctggtgaggt
     1381 gatttttaaa cacaaagact ttgcaggaat tcactttaca ggttctacag gtgtgtttag
     1441 aaacttgtgg aaaaccattg gtgaaaattt ggataaatac aaatcatacc ctcgtatcgt
     1501 tggagaaact ggaggtaaag atttcgtagt tgctcataaa tctgctaacg ctgccgaggt
     1561 tgcaactgct ttgtcaagag gtgcatttga ataccaagga caaaaatgtt cggctgcatc
     1621 gcgtgcttac atcccgtcta acttgtggaa cgaagtgaaa gaaattttgg tgagccaggt
     1681 ttcttcaatg aaaatgggat cgccagaaga cacttccaac ttcataaatg cggtgattga
     1741 taaaaaagct tttggtaaaa tcacagaata cattgaatac atcaaaaatg cttctgatgc
     1801 tgaaattgta gttggaggaa attacgacgg aagtaaagga tatttcattg aacctacggt
     1861 ttgtgttaca accaatccga agtttaaaac aatggaagaa gaaatttttg ggcctgtctt
     1921 gacaatttat gtttacgacg aaaacaaata cgaagaaaca ttgaaattgg tggacgaaac
     1981 ctcagaatat gcattaacag gttcaattat ggcaaccgat cgttatgcaa ttgatctggc
     2041 tgtgaacatg ttgcgcaatg ctgcaggaaa cttctacatc aatgacaaac caacaggagc
     2101 tgttgtaggt caacaaccat ttggtggtgc tagaggttct ggaacaaatg ataaagctgg
     2161 ttctgcaatg aatttaatgc gttgggtttc tttgcgtaca attaaagaaa catttgtaac
     2221 accaaaagat tacaaatacc catttttagg ttaatctgaa tataccaaat atcagttttt
     2281 gatttaataa acgaaggagc tatctgaatt agatagttcc tttttttatg cttcaaactt
     2341 ctcgcccttg gcttaactac aaaaaaaaga gtaagaagct aatcctactc tttcctagtg
     2401 atcccgctgg gattttagtt agttaaaa
//
LOCUS       DAOQVQ010000385         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_53510_length_2418_cov_3.039780, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000385 DAOQVQ010000000
VERSION     DAOQVQ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53510_length_2418_cov_3.039780"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..945
                     /locus_tag="PKN22_09960"
     CDS             142..945
                     /locus_tag="PKN22_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43071.1"
                     /translation="MARQKGVLKYVGTLGDVRHFKIKGQEGYFAGMVGGPSGEQVLSA
                     PEFERTRENMNEFGACAKAGKSVRTGLSQIVKQMSDTQVAGRLTAIMKKINLEDQTEA
                     RGYRKIEITTQRNYLVGFEFDKNTSLNGIFNAPYSVTHDPARNTADFVIPAFNPANLI
                     NAPAGATHFRLIQTLSVVSDFAYNAVTNSYDPIDTAQNEVSDVQFSGFLDLYAPATAT
                     TITATLPNGITPSADVTVLQGIGIEFYQKVGSNYYLFATGNCLKIEDAF"
     gene            1014..1466
                     /locus_tag="PKN22_09965"
     CDS             1014..1466
                     /locus_tag="PKN22_09965"
                     /inference="COORDINATES: protein motif:HMM:NF040141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HNS43072.1"
                     /translation="METKIIRVAQGKQSTLSQLYINGVFQCYLLEDKIRTEKIKEHTA
                     IPTGTYSLQLNTWGAKNVNYKKAFGKVHQGMIEITGLPNFSYVYIHTGNTIEDTAGCP
                     LCGFGFQLIQGDYRVTQSVMAYQMIYPKLVALAKNKANKISIENNFQF"
     gene            1479..1757
                     /locus_tag="PKN22_09970"
     CDS             1479..1757
                     /locus_tag="PKN22_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43073.1"
                     /translation="MEQAPQLMVLFGSLISLLLSVVAYFIKQLHTDFKRMEKDLVEVK
                     TMALLIKTEFKSSSDLLNQKVDYLEHRVQKLEMLCIHEHQIETIKKNK"
     gene            1802..2044
                     /locus_tag="PKN22_09975"
     CDS             1802..2044
                     /locus_tag="PKN22_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43074.1"
                     /translation="MNIVQRVKAPTPKFFKVLRTIGLALATVGGTIIASPIALPAGLV
                     AAAGYLVLGGGVLSAVSQTAVQDDEEPRVPQEDRVQ"
BASE COUNT          777 a          403 c          483 g          755 t
ORIGIN      
        1 caatgggttt ccactaaata gggtgctttt gcgttgtatt gcattgattt tcagtatttt
       61 aagttattgt aaatcaaaga taatgaatga tatttgagta taaaatttca cttaaatatt
      121 aacttctaaa atctttacaa aatggctaga cagaaaggcg ttttaaagta tgtgggaact
      181 cttggagatg ttcgccactt taaaattaaa ggacaagaag ggtactttgc cggaatggta
      241 ggtggacctt caggggagca agttttatca gctccagaat ttgaaagaac tcgtgagaac
      301 atgaacgagt ttggtgcttg tgctaaagca ggaaaatccg ttagaactgg tttatcacaa
      361 atcgtgaagc aaatgtctga tacgcaagta gcaggacgtt taacggctat catgaagaaa
      421 atcaatttgg aagaccaaac cgaagccaga gggtatcgca aaattgagat taccactcaa
      481 agaaattact tggtaggttt tgagtttgac aaaaacactt ccttaaatgg tattttcaat
      541 gctccttaca gtgttacgca tgacccagca agaaacactg ctgattttgt aattcctgcg
      601 tttaatccag cgaatttgat taatgctcct gcaggtgcta ctcactttag attgattcaa
      661 actctttcag tagtaagtga ctttgcttac aatgctgtaa cgaatagtta tgatcctatt
      721 gacacagctc aaaatgaagt gagtgatgtt caatttagtg gtttcttaga cttgtatgct
      781 cctgcaactg caactaccat tacagcaact ttgccaaatg gtatcactcc atcagctgat
      841 gttaccgttc ttcaaggtat tggaattgag ttctatcaaa aggtaggttc aaactattac
      901 ttgtttgcaa ctggtaactg cttgaaaatt gaggacgcat tttaagaatc ctacttacaa
      961 atccctcaga acctctgggg gattttttgt tttaacttac taattcaatt cccatggaaa
     1021 caaaaatcat tcgcgtagca cagggcaaac aaagtacatt aagccaatta tatatcaacg
     1081 gagtatttca atgttattta cttgaggata aaatacgaac agaaaaaatc aaggaacata
     1141 ctgccatacc tactggaacc tattcattgc agcttaatac ttggggtgca aaaaatgtca
     1201 actacaaaaa agcttttgga aaagttcacc aaggtatgat tgaaattaca ggtttaccaa
     1261 atttcagtta tgtgtatatc catacaggaa atacgattga agataccgca ggatgtccgc
     1321 tttgtggatt tgggttccaa cttatccagg gcgattaccg agttacgcaa agtgttatgg
     1381 cttaccaaat gatttatcct aagctagttg cattagcaaa gaacaaagca aacaagatta
     1441 gtatcgaaaa caatttccaa ttttaaactt gtaatgccat ggaacaagct ccacaattaa
     1501 tggtactttt tggaagttta ataagcttat tattatcggt tgtagcctat tttatcaaac
     1561 aattgcacac agatttcaaa agaatggaaa aagaccttgt tgaagtaaaa accatggcat
     1621 tattaatcaa aaccgagttt aaaagcagca gtgatttact gaaccaaaaa gtagattatt
     1681 tagaacaccg agtacaaaaa ttagaaatgc tttgcattca cgaacaccaa atcgaaacaa
     1741 ttaaaaaaaa taagtgagta attatccatc tttaaaaatc acgaacatga aaagtaatga
     1801 aatgaatatc gttcaaagag tgaaagctcc aacaccaaag tttttcaaag tattgagaac
     1861 aattggttta gccttggcaa ctgttggagg aactatcatc gcttcaccaa ttgcattgcc
     1921 ggctggatta gttgcagctg caggttattt ggttttgggc ggtggtgtct tgtcggcagt
     1981 atcgcaaacc gctgtacaag atgacgaaga accgagagtt ccacaggaag acagggttca
     2041 ataaaactaa aattgcttaa aaattgcaat caaaagctcc tatttcaggg gctttttttt
     2101 gttttatacc caattgcttc gctttttcca attttattta attggtgcgt ttatattgcg
     2161 gttatgtatt gttttgcttc gggataggga ttgtttgggg aatttttttg aaaagaaaaa
     2221 acctagccca tcggggccag gtttgtgtta tttgtttctg tagcgttgca tgatggcgaa
     2281 acggagctca ctgataagat gtaagttttt gttgtggtgg ttttcttgtt cttgcagcag
     2341 ctttagagct tgaatgtgtt taaggtgctg ttcgtgttct ctcatcaaac gggtggcttt
     2401 tggattgaac tctttttt
//
LOCUS       DAOQVQ010000386         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_53832_length_2409_cov_4.247664, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000386 DAOQVQ010000000
VERSION     DAOQVQ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53832_length_2409_cov_4.247664"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKN22_09980"
     CDS             <1..359
                     /locus_tag="PKN22_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43075.1"
                     /translation="MTVELLQSGYFLIATEVKKEVKDLTLAEIKYEIDRLRTKLVPQE
                     ELDLVKNYLLGQLLKSADGPYSMMDLFLSVEEYGLNLEYYNKLIHDIQSITPERIKEL
                     AVKYLDWNEFTIVTVG"
     gene            359..748
                     /locus_tag="PKN22_09985"
     CDS             359..748
                     /locus_tag="PKN22_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNS43076.1"
                     /translation="MEFKEIIGAVEQFHDAFKIENNHQPTASIGEDQIDLRFRLMQEE
                     NEEYLQAAKQGDVVEVADALGDMLYILCGTILKHGMQHKIEEVFQEIQRSNMSKLDEN
                     GNPIYREDGKVMKSELYFKPNIAEIIK"
     gene            765..2321
                     /locus_tag="PKN22_09990"
     CDS             765..2321
                     /locus_tag="PKN22_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNS43077.1"
                     /translation="MEKFKKGLLTFFHTYFYEALFVLIAIIAIVLQHNYLHLYPRSLH
                     AWAQSDLYALAKGYLNNGFDLLHPEGYVYNKQFPYYWNYPYDSTITGGNLPLHQYFIA
                     LLMALFNNDGPFIFRTYVLIFSLIGFYYLLQLAKLYKINAAATIFLVSIALFSPIYFY
                     YQASFLIVIPCIATTFMGLYYYFLHLKINQIKHWNIAVVILFLTLIVRTTYAIPLIAM
                     LLHQLLIGFQTKKFQFKKIIVVGLMGLVWGAITMHIHQLSAQHGSYFLAQILPAENWQ
                     QFLKLLSEAINFWNWNYFSPNQVGVFVVLFTISLFFLRKKHVTNLKGMGYFVAILGIG
                     YCMFIYAMISQFHDHDYYFLDTAYIFFILLMVASFSLIKHTNKTIQIIAVLTALLFIN
                     IFKNGTKQAVEGRYFNIHPKDIVSRTSIDYENSVAFINNLKIEASQKIGVLGFEASQL
                     PFLFIGKKGYAINCEDEQSFNKMINWGIDYVFCSNSELQQKVQNHSFVQSRLQLIATN
                     GKISYFKVIH"
BASE COUNT          789 a          375 c          413 g          832 t
ORIGIN      
        1 ccatgacggt tgaactgttg caatctggtt attttcttat tgctacggaa gttaaaaaag
       61 aagtcaaaga tttaaccttg gccgaaatta aatatgaaat tgaccgttta cgcactaaat
      121 tggttccgca agaagaattg gatttagtaa aaaactattt gttgggacaa cttttaaaaa
      181 gtgcagacgg accttattcc atgatggatt tatttctttc ggttgaagag tatggtttaa
      241 acttggaata ttacaacaaa ttgatacatg acattcaatc catcacgcca gagagaatta
      301 aagaattggc tgtaaaatat ttagattgga atgaatttac aatagtaact gtaggataat
      361 ggaatttaaa gaaataatag gagctgttga gcagtttcac gatgcattta aaatagaaaa
      421 caaccaccaa ccaacggcaa gcattggtga agatcaaatt gatttgcgtt ttcgattgat
      481 gcaggaggaa aatgaagaat atttgcaagc tgcaaaacaa ggcgatgttg ttgaagttgc
      541 cgatgcattg ggcgatatgt tgtatatttt atgtggcact attttaaaac acggtatgca
      601 gcataaaatt gaagaagttt ttcaagaaat tcaacgttcc aacatgagta aattggatga
      661 aaacggaaat cctatttacc gcgaagatgg caaggtgatg aaatctgagt tgtattttaa
      721 gcctaacatt gcggagatta ttaaataaca agcagcttta aaaattggaa aaattcaaaa
      781 aagggcttct aacgtttttt cacacctatt tctatgaggc tctttttgta ttgatagcca
      841 tcattgctat tgtgttgcaa cacaattatt tgcatttata tccacgttca ttgcatgcgt
      901 gggcacagtc ggatttatac gcactagcaa aaggttatct caacaatgga tttgatttgt
      961 tgcatcctga aggatatgtg tataacaagc aatttcctta ttattggaat tatccttacg
     1021 attcaaccat caccggtgga aatttgccct tgcatcaata tttcattgcg cttttaatgg
     1081 ctttatttaa caacgatggc ccgtttattt tcagaaccta tgttttaatt tttagtttaa
     1141 ttggttttta ctatttactc caacttgcaa agttgtataa aataaacgcg gctgccacca
     1201 ttttcttggt ttctatcgct ttgttttcgc ccatttattt ttattatcaa gctagttttt
     1261 tgattgtaat tccctgcatt gcaacaactt ttatggggct gtattattac tttttacatc
     1321 tgaaaatcaa tcaaataaaa cattggaaca ttgctgttgt tatacttttt ctgacactta
     1381 ttgttcgtac aacctacgca attccattaa ttgcaatgtt gctgcatcaa ttgctaattg
     1441 gatttcaaac taaaaaattt caattcaaga aaataattgt tgtggggtta atggggctag
     1501 tttggggtgc aattactatg catattcacc aactttcggc acaacatgga tcttattttc
     1561 tggcacaaat tctcccagca gaaaactggc aacaattttt gaagttattg tcagaagcta
     1621 ttaatttttg gaactggaat tatttttcac ccaatcaagt aggtgttttt gtggtactat
     1681 ttacaattag tttgttcttt ttgcgaaaaa aacacgttac caacctaaaa ggaatggggt
     1741 attttgttgc gattttagga atagggtatt gtatgttcat ttatgctatg atttctcaat
     1801 ttcatgacca cgactattac tttttggata ctgcctacat cttttttatt ttattgatgg
     1861 tggcttcatt ttcattaatc aagcatacaa ataaaacgat acaaattata gctgttttaa
     1921 cggcattatt gtttatcaat atttttaaaa acggaaccaa acaagcagtt gaaggacgat
     1981 atttcaacat tcatcccaaa gacatcgttt cacgtacatc cattgattat gaaaatagtg
     2041 ttgcatttat taacaacttg aaaattgaag catcacaaaa aattggagtc ttgggatttg
     2101 aagcatcaca acttcctttt ctttttattg gtaaaaaagg atatgccatt aattgtgaag
     2161 atgaacaatc atttaataaa atgatcaatt ggggaattga ttacgttttt tgttccaatt
     2221 cagagttgca acaaaaagta caaaaccatt cttttgttca aagtcgctta caactgattg
     2281 ctacaaatgg aaaaattagt tatttcaagg ttattcacta agtatttcat tcaccgtagt
     2341 atatttgtaa acaaaaacaa gacaaaacaa ttgaaagcta aagaattaat ccataaactt
     2401 atcccagca
//
LOCUS       DAOQVQ010000387         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_54170_length_2400_cov_3.231557, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000387 DAOQVQ010000000
VERSION     DAOQVQ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54170_length_2400_cov_3.231557"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1268)
                     /locus_tag="PKN22_09995"
     CDS             complement(99..1268)
                     /locus_tag="PKN22_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS43078.1"
                     /translation="MEQLVAHSAKLIAETELVFVRYLYEKINWNNRQIGLVGPRGVGK
                     TTLILQHIKSKLPHHQTLYVTAEDFYFSTNKLIDLATTFVQLGGKYLFIDEIHKYPNW
                     SKELKLIYDYHSELHVVFTGSSVLDIKKGSSDLSRRSVVYQMQGLSFREYLAIFHSIE
                     LPTYSLDDVLNHRVDFSVVKHPLLYFSDYLKNGYYPFAREEDFDVKLKQIINQTLEVD
                     IPFFSNMNVATGRKLKQLLAIVSESVPFKPNMSKLAEMIGVSRNNMTDYLYFLEEAGM
                     IIQLKADTGGIRNLGKVDKIYLDNSNLIYSLVGNKLNIGNVRETFFINQLKCIAPVNS
                     SSLVDFTVNNLEFEIGGKKKSAKQLQSHQQGYVVKDEIETGFASVIPLWHFGMLY"
     gene            complement(1528..1791)
                     /locus_tag="PKN22_10000"
     CDS             complement(1528..1791)
                     /locus_tag="PKN22_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43079.1"
                     /translation="MKKVGVVVASLMLVFSCSNKDKQFCECLEVGNELNQEAAKYSSI
                     ELDKITDKDVAKLKALKEKKEKICAPYETMAGPEMLKKQEACK"
     gene            complement(1791..>2400)
                     /locus_tag="PKN22_10005"
     CDS             complement(1791..>2400)
                     /locus_tag="PKN22_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNS43080.1"
                     /translation="AAAIGIGIDVEEPMGNMIIDIGGGTSEIAVIALGGIVCDKSIRV
                     AGDDFSADIEDYMRRQHNILVGERTAELIKIEVGSALPELDNPPADFAVRGRDLMTGI
                     PKEIVVTYSEIAHALDKTISKIEEAILSALEMTPPELSADIYKTGIYLAGGGSQLRGL
                     DKRISARTKLPVHIAEDPQRAVVRGTAIALKNIGRYVFLIKD"
BASE COUNT          780 a          459 c          347 g          814 t
ORIGIN      
        1 aaaatttgtt ttcttacctg gcgttattac agaagaaaac atggtggaaa ttcctttgtt
       61 gaacaaaaaa ggtgtgttga ttaaatagaa aattgagttt aatacaacat tccaaaatgc
      121 cataatggaa taactgatgc aaaaccagtt tcaatctcat ctttaacaac gtatccttgt
      181 tgatgtgact gcaattgttt agcacttttt ttctttcctc cgatttcaaa ttccaggttg
      241 ttaactgtaa aatctacaag agaggatgaa tttacaggtg caatacattt taattgattg
      301 ataaaaaacg tttctctaac atttccaatg ttaagtttat taccaaccaa gctgtatatc
      361 aaatttgaat tatccaaata gattttatca acttttccaa ggtttcttat gcctccagtg
      421 tctgctttta attgaattat catgcccgct tcttctaaaa aataaagata atccgtcata
      481 ttatttctac taactccaat catttcagct agtttactca tattgggttt aaaaggcaca
      541 ctttctgaaa caattgcaag taactgtttt aatttacgtc ctgtagcaac attcatgtta
      601 gaaaaaaaag gaatatccac ttctaacgtt tgattgataa tttgcttcaa ttttacatca
      661 aaatcttctt cacgggcaaa tggataataa ccatttttca aataatcaga aaaataaagt
      721 aaagggtgtt ttacaactga aaaatctaca cggtgattca atacatcgtc caaggaatag
      781 gttggtaatt caatagaatg aaaaatagcc aaatactctc taaacgacaa tccttgcatt
      841 tgataaacaa cacttcttct acttaaatcg gaagatcctt ttttaatatc caatacagaa
      901 gaacctgtga aaacaacatg caattcggaa tggtaatcgt aaatcaactt taattcttta
      961 gaccaattag gatatttgtg aatttcatcg atgaacaaat atttacctcc caattgaaca
     1021 aacgttgttg ctaaatctat taatttattg gtcgaaaaat agaaatcttc tgctgtaacg
     1081 tataatgttt gatgatgtgg gagttttgat ttaatgtgtt gtaaaataag tgtggtttta
     1141 ccaacacctc ttggccctac caaacctatt tgcctattat tccaattaat tttttcatac
     1201 aaatagcgca caaaaacaag ttctgtttct gcaattaatt tggctgaatg tgcaactagt
     1261 tgttccataa cactacaaaa atacactttt tgcactatta tagtgcattt attttatttt
     1321 tttgcactat catagtgcat ttagggaaat aatcataaaa aaacctgaga ttcgatggcc
     1381 gctgttacac ttggtctatc tcaggttaaa ctctgcaaga atataaaacc tatttacttt
     1441 attgacgcaa caattttaaa aaggttgggt tgatacataa aaaaaggtga aattttcatt
     1501 ccaccttttc aattatttaa tcgaaactta tttacaagct tcttgttttt tcaacatttc
     1561 aggtccagcc attgtttcgt aaggagcaca aattttttct tttttttctt ttaaagcttt
     1621 caattttgca acatctttat cggtaatctt gtccaactct atggaactat atttagctgc
     1681 ttcttggttc aattcattgc ctacctccaa acactcacaa aactgtttat ccttgttgga
     1741 acaagaaaaa acgagcatca aactagctac aacaacgcca acttttttca ttaatctttg
     1801 attaagaata cgtaacgacc aatatttttc aatgcgattg cagttccacg aacaacggct
     1861 ctttgtggat cttctgcaat gtgcactggc aattttgtgc gagcagaaat acgcttatcc
     1921 aaacctctca actggctacc acctcctgct aaatagatac ctgttttata gatatcggca
     1981 gataattcgg gtggtgtcat ttccaaagca cttaagatgg cttcttctat ttttgaaatg
     2041 gttttatcca atgcatgtgc aatttcagaa taggttacca caatttcttt tggaataccc
     2101 gtcattaagt cacgccctcg aacagcaaag tctgctggtg gattatctaa ttctggcaag
     2161 gctgaaccta cttcaatttt aattagctca gcagtgcgtt cacccaccaa aatattgtgt
     2221 tgacggcgca tgtaatcttc aatatctgca ctaaaatcgt cacccgcaac acgaatggat
     2281 ttatcacaaa caataccacc taaagctatt acggcaattt cggaagtacc accacctatg
     2341 tcaataatca tatttcccat aggttcttcc acatcgatac caataccgat tgcagcagcc
//
LOCUS       DAOQVQ010000388         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_54215_length_2399_cov_2.787969, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000388 DAOQVQ010000000
VERSION     DAOQVQ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54215_length_2399_cov_2.787969"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKN22_10010"
     CDS             <1..441
                     /locus_tag="PKN22_10010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNS43081.1"
                     /translation="PAVAGICALIWDANPYLSPRQVKQIIIETARLDQYTGQIPAIGD
                     VKWGHGKINALAAVKKALTIVGLEDNVATIAKWTVYPNPSSATIHLQGLSNITSVELI
                     DLSGKQHALNVQQQSWVVSKFTPGVYIFRTVANEKVYQQKIIIQ"
     gene            complement(442..1539)
                     /locus_tag="PKN22_10015"
     CDS             complement(442..1539)
                     /locus_tag="PKN22_10015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS43082.1"
                     /translation="MIGATLAYCIIYFLVAIAAYVGTQKMQFKNKNLAQELNSMGEIT
                     LIVPFRDEAKRILPLLQSLSASNQLPKEILFVNDHSCDQTIEVITNHLKIDNYRIIHS
                     QKDGKKAAIHAGVEQCLTPYFLTMDADISFGNDYFDKLFQLAAVDMAILPVKMQSKGG
                     FSLFELDVYLVNVLNSIVAGYYRPIVASGANLLVKKTAYLASNTMEHHAHILSGDDQF
                     LLNDFVQSNKIVAVYTNKQLAVTTPSPASVKDLINQRLRWIQKTPHVNDGLAKQIGII
                     HLGWMLILSVLVTILLLQSHYSTALFLIISKSAIDAWVSFSYFKSIEKLRYLVLLPLY
                     EMCFPIYTLLLAILSLFYQPKWKQRPTLSKQ"
     gene            complement(1536..>2399)
                     /locus_tag="PKN22_10020"
     CDS             complement(1536..>2399)
                     /locus_tag="PKN22_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNS43083.1"
                     /translation="IILTLAVGIIFYFQFKKLSINQLNDFKIHHYFSLALLLVLTFPN
                     YFLEYKKWEYILQSLKISADKSIKIQSFFAGIITGMLTPNMQGNFIGRIYYFQRRYRI
                     SIVLLTLTANLGQFIATIVLGVFGLVLSSKIVASNLLFFPLFITLISLLFYFNYEKIQ
                     FFSKQFKWYKRIAKVLQTNARFRLQILLLSLLRHLVFALQLLLAFHAFGEPMTWHLFW
                     LIWLFYLWTTLSPSLFLGKLFIRESIALLVFTGLSISPTSIVMVSLTVWVFNLFLPTL
                     IGLIICKKKQK"
BASE COUNT          882 a          414 c          407 g          696 t
ORIGIN      
        1 cccgctgttg ctggaatttg cgctttaatt tgggacgcaa atccctattt atcaccgcgt
       61 caagtgaaac aaatcatcat agaaacagct cgtttggatc aatatacagg tcaaatacca
      121 gccataggtg atgtgaaatg gggacatgga aaaatcaacg cgttggcagc tgtgaaaaaa
      181 gcgttaacca ttgttggact tgaagacaat gttgcaacaa tagcaaaatg gacggtatat
      241 cctaatccat catcagcaac aattcatctt caaggtttaa gcaacataac atccgttgaa
      301 ttgattgatt tgtcgggcaa gcaacatgcg ctaaatgtgc aacaacaatc atgggtcgta
      361 agtaaattta caccaggtgt ttacattttt agaacggtag caaatgaaaa agtttaccaa
      421 caaaaaatca ttattcagta gctattgttt ggatagggtg ggacgctgct tccactttgg
      481 ttgataaaac aacgataaaa ttgcaagcaa caacgtgtaa ataggaaaac acatttcgta
      541 taaaggaagc aacaccaaat aacgtagttt ttcaatagat ttaaaatacg agaaagaaac
      601 ccaagcatca attgcgcttt ttgaaatgat taaaaatagc gcagtagaat agtggctttg
      661 caacaaaaga atcgtcacca atacgctcag tattagcatc caacctaaat gaataatgcc
      721 tatttgtttt gccaatccat catttacatg cggggttttt tgaatccatc gtaaccgttg
      781 atttatcaaa tctttcacgg atgcaggcga aggcgttgta acagcaagtt gtttgttggt
      841 atatacagca actattttgt ttgattgcac aaaatcgttc aataaaaatt gatcgtcgcc
      901 actcaatatg tgtgcatggt gttccattgt attagaagcc aaataggctg tttttttaac
      961 caataaattg gcaccactcg ccacaattgg acgataatag ccagcaacaa tactgttcaa
     1021 aacattaact aaataaacat ccaactcaaa aagagaaaac ccacctttcg attgcatttt
     1081 tacaggaagt atagccatat caacggctgc taactgaaat aatttatcaa aataatcatt
     1141 gccaaatgaa atatcggcat ccatggttaa gaaatagggg gttaaacatt gttcaactcc
     1201 agcatgtatt gccgcttttt ttccatcttt ttgagaatga ataatccgat aattatcaat
     1261 ttttaaatgg ttcgtaatca cttcaatagt ttgatcgcaa gaatgatcgt tgacaaacaa
     1321 aatttcttta ggcaattgat tggatgcaga caacgattgc aataaaggta aaatgcgctt
     1381 ggcttcatct ctaaatggaa ctatgagtgt tatttcaccc attgaattca attcttgagc
     1441 aagatttttg tttttaaatt gcattttttg ggtaccaaca tacgccgcta ttgccactaa
     1501 aaaataaata atacaatatg ccaacgttgc accaatcatt tctgtttctt tttgcaaata
     1561 attaacccga taagtgtagg taaaaataga ttaaaaaccc ataccgtcaa cgaaaccatt
     1621 acaattgaag taggtgaaat actcaatccc gtaaaaacca ataaagcaat ggattctcgg
     1681 ataaataatt ttcctaaaaa caaagatgga gaaagcgttg tccacaaata aaacaaccaa
     1741 atcaaccaga ataaatgcca agtcatcggt tctccaaatg catgaaaagc aagcaataat
     1801 tgcaaagcaa aaaccaaatg tctaagtaaa gataatagca aaatctgtag cctaaaacgc
     1861 gcgttggttt ggagtacctt tgctatgcgc ttataccatt tgaattgttt ggaaaagaat
     1921 tgtatttttt cataattgaa gtaaaacaat aggctaatca gggtaatgaa taaaggaaaa
     1981 aacagcaaat tggaagctac aatttttgac gataaaacca gcccaaaaac acctagaact
     2041 atagttgcta taaattgccc taaattggct gttaaggtta gcaacacaat agaaattctg
     2101 taccttcgtt gaaaataata gatacgacca ataaaatttc cttgcatgtt gggcgttagc
     2161 attccagtta tgatgccagc aaaaaaagat tgtattttta tagatttatc agctgaaatt
     2221 tttaatgatt gaagtatgta ctcccatttc ttatattcta aaaaatagtt aggaaatgtt
     2281 aacaccaaca aaagcgctaa actaaaatag tgatgaattt taaaatcatt taactgatta
     2341 atagatagtt ttttaaattg aaaatagaag ataataccaa cagcaagtgt tagaattat
//
LOCUS       DAOQVQ010000389         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_54346_length_2395_cov_3.226923, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000389 DAOQVQ010000000
VERSION     DAOQVQ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54346_length_2395_cov_3.226923"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKN22_10025"
     CDS             complement(<1..885)
                     /locus_tag="PKN22_10025"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43084.1"
                     /translation="MKIFTFFCSLLVASTTFSQIRKNTSIKFEDLKVQKAEYGIKDFK
                     RYNYLLNYVNPFIGTGGHGHTYPGASAPFGMMQLSPDTRYNGWDGCSGYHYSDDVIWG
                     FSHTHLSGTGVEDYCDLLIVPQTGKPRTTPGYKDSLEGYGSKFSHANEKATPGYYSVK
                     MDNGIFAEFTVSKRTGMHHYSFPKNGEKRFILIDLTHRDQLTKYFINQEGREISGARF
                     SSSWAKDQRLFFYIQTNVDAESSELLDNGSKLILQFPENVTAIELKVGLSAVDEQGAK
                     NNVIYELKGKNFASLKEAV"
     gene            complement(936..1343)
                     /locus_tag="PKN22_10030"
     CDS             complement(936..1343)
                     /locus_tag="PKN22_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43085.1"
                     /translation="MNFKSKSSFFVLSLLVIIGLYAGYRYFSNAQVYNMLPKTITLNL
                     KNKNAVYLLEKHTQQSGVYQLEMTFQGRLSDNITVHLSKDGIVGLQSIRIKKGNVKTS
                     FIARWENDNAYLLIENPAQSENQLELEYQFVSE"
     gene            complement(1346..1612)
                     /locus_tag="PKN22_10035"
     CDS             complement(1346..1612)
                     /locus_tag="PKN22_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNS43086.1"
                     /translation="MENKQQPQEKVAFWSKVKIAVATLLLLFVLLIIIKNWNYVSINL
                     VFKSFDLPLTFILFIALAIGYFWGTIGSYFKQKRNSKKENLSNE"
     gene            1788..>2395
                     /locus_tag="PKN22_10040"
     CDS             1788..>2395
                     /locus_tag="PKN22_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNS43087.1"
                     /translation="MNKNWKTIGLGVLGGLLPITALLLINTTSLKPSTGSTLFNEAPV
                     RQVSYTGTATPTDFIQASENSINSVVHVTTKVVQTTFQRDLFQEFFYGPGAGGREFKQ
                     YGSGSGSGVIVSADGYIVTNNHVIDNASEIEVILNDNSKYSAKIVGTDPSTDIAVLKI
                     EASNLSPIVLGNSDDVKVGEWVLAVGNPFNLTSTVTAGIISA"
BASE COUNT          781 a          400 c          412 g          802 t
ORIGIN      
        1 taccgcttct tttaatgaag caaagttttt accttttaat tcatagataa cattgttttt
       61 agctccttgc tcatccaccg ccgaaagtcc aacttttagc tcaatagccg taacattttc
      121 aggaaattga agtattaatt tagaaccatt gtccaacaat tcagatgatt cagcatctac
      181 attggtttga atgtaaaaaa acaaacgttg atcctttgcc catgaagatg aaaaacgagc
      241 acccgaaatt tctcgcccct cttgattaat gaaatattta gttaattgat cgcggtgtgt
      301 taaatcaatt aaaataaagc gtttctcacc attttttgga aaagaatagt ggtgcatgcc
      361 tgttctttta gaaacagtaa attcagcaaa aattccgttg tccattttta cagaataata
      421 acccggcgtt gctttttcgt tggcatgtga gaatttagaa ccatatcctt ccaaactatc
      481 tttataacct ggagttgtgc gtggtttccc tgtttgaggg acgattaata aatcacaata
      541 atcctctaca cccgttccac ttaaatgggt gtgactaaat ccccaaataa catcgtctga
      601 ataatggtaa ccagaacagc catcccagcc gttgtagcgt gtatctggac ttaattgcat
      661 cattccaaac ggggcgcttg caccagggta ggtgtgtccg tgtccgcctg taccaataaa
      721 cgggttgacg taattcaata aataattata tcgcttaaaa tctttaatgc catattcggc
      781 tttttggact tttaaatctt caaattttat ggaagtattc tttctgattt gagaaaatgt
      841 agtggatgct acaagtaagg aacagaaaaa ggtgaaaatt ttcatttttt ttaatgtttg
      901 agaatgtaaa ttttataaaa cacaaagata aacttttatt cggatacaaa ttggtattcc
      961 aattccaatt gattttctga ttgtgccgga ttttcaatca ataaataagc gttgtcattt
     1021 tcccatcggg caatgaaact agtttttaca tttccttttt tgatacgaat ggattgcaat
     1081 ccaacaatgc catctttact caaatgaact gtaatattgt cagataaacg accttgaaaa
     1141 gtcatttcta attgataaac accactttgc tgagtatgtt tttcaagcaa ataaacggca
     1201 tttttgtttt ttaaattaag cgttattgtt ttgggtagca tgttatatac ttgtgcattt
     1261 gaaaagtaac gatacccagc atacaaacct ataataacaa gtaaacttag aacaaagaac
     1321 gaacttttag atttaaaatt catgtctatt cattggataa gttttccttt tttgaattgc
     1381 gtttttgctt gaaataggaa ccaattgttc cccaaaaata acctatagcc aatgcgataa
     1441 aaagaatgaa cgtcaatggt aagtcaaatg atttgaatac caagttgata gaaacgtaat
     1501 tccagttttt tatgatgatt aaaagcacaa acaataacaa tagtgttgcc actgctattt
     1561 ttactttgct ccaaaatgct actttttctt gaggttgttg cttgttttcc attttttata
     1621 taaatcttag taacgaaatt aatgaattaa agttaatagt ggtataaatc ggtttgtttt
     1681 tttaatgggt tgacttaaca atctttaaca ctgacatttt aacattttgg caatattggt
     1741 gttataattg tgtaaataaa aagtgaaaac aaattaaata taaaattatg aacaagaatt
     1801 ggaaaactat aggtttaggc gtgcttggag gacttcttcc aattacagca ttgttattaa
     1861 ttaataccac ttcattaaaa ccatcaactg gttcaacact attcaatgaa gcgccagttc
     1921 gtcaggtaag ttacacagga accgcaacac caacagattt tattcaagca tcagaaaatt
     1981 caattaactc ggtggtgcac gttacaacta aagtcgttca aacaacattt caacgcgatt
     2041 tgtttcaaga gtttttctac ggacctggag ctggtggacg tgagtttaaa caatacggat
     2101 cgggttctgg ttcgggtgtt atcgtttcgg cagatggcta cattgtaaca aataatcacg
     2161 ttattgataa tgcaagcgag atagaagtta ttttaaatga caactcaaaa tacagtgcaa
     2221 aaattgtcgg tacagaccct tcaactgata ttgcagtatt gaaaattgaa gcttctaact
     2281 tatcacctat cgtattagga aatagcgatg atgttaaggt aggtgagtgg gtattggctg
     2341 ttggaaatcc gtttaattta acatcgacag tgacagcggg tattatttct gcaaa
//
LOCUS       DAOQVQ010000390         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_54760_length_2383_cov_4.262457, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000390 DAOQVQ010000000
VERSION     DAOQVQ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54760_length_2383_cov_4.262457"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..>2383)
                     /locus_tag="PKN22_10045"
     CDS             complement(158..>2383)
                     /locus_tag="PKN22_10045"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNS43088.1"
                     /translation="SPTITNSTIANNSAFNGGGMFNENSSPTITNSTIAANNSAYYAG
                     GMYNSSSSPTITNSTITNNLASDGGGMFNDYSSPTITNSTIANNSAIYSCGGMYNYYY
                     SSPTITNTTFANNSAYYAGGMYNYYYTSPAINNSILFGNTASNNAVMLSAQNSTPIVK
                     NSLIEGISWNATNWGTDGGGNILSTTNPFVDAANENYRLKPCSAPVNAGNNTLYGSTL
                     FSDSDLAGNPRLFENTIDMGAYEVQVPSYLTVNLGVDTAICSGTSLAVDAQNASSTYL
                     WNDNSTNQTLAITSAGTYSVIVTDANGCVGKDTIVVTENALPVVNLGADDSYCHGTTY
                     ASTLDAQNAGSSYLWNDNSTNQMLSITAAGTYSVIVTDANGCVGKDTIVVTENALPVV
                     NLGADDSYCHGTTYANTLNAQNAGSAYLWNDNSTNQTLTITTTGTYSVIVTDANGCVG
                     KDTIVVTENALPVVSLGADDSYCHGTTYANTLNAQNAGSTYLWNDNSTNQMLTVTTAG
                     AYSVIVTDANGCVGKDTIVVTENALPVVSLGADDSYCHGTTYASTLDAQNAGSSYLWN
                     DNSTNQTLTVTTTGTYSVIVTDANGCVGKDTMVVTENALPTVAITTTQNELCVYNASI
                     ALSGTPANGIFSGNGVSNSNFDPSVAGIGNHDVVYTFTDANGCTNNDTLAITVDGCAG
                     LEDVTTTNLSVFPNPSETGVFNIVSSQALSGEAIVVLNALGQQVAVYSFNGTEKTIDL
                     S"
BASE COUNT          708 a          450 c          434 g          791 t
ORIGIN      
        1 ccaactctca aaaacaccaa aagcgaccaa attaaagtcg cttttgaatg ctgaaatgaa
       61 gtgaaaaagt gaagattcta cttttttctt ctgtttattt tttaatcaat ttgaaagttt
      121 gtgtacctac tttcaaaaga tagattcctt ttgcttctta agacaaatcg attgtttttt
      181 ctgttccatt gaacgaataa acagctactt gttgtcccaa tgcgtttaaa acaacgattg
      241 cttcacctga aagtgcttga gacgaaacaa tgttaaacac tcctgtttca cttggatttg
      301 ggaaaacgga taagttagtt gttgttacat cttccaatcc agcacatcca tccacagtaa
      361 tggctaaggt atcgttgtta gtacagccat tcgcatctgt gaatgtataa acaacgtcgt
      421 ggttaccaat tccagcaaca gatggatcaa aattgctgtt tgaaacgcca tttccagaga
      481 aaattccatt ggctggtgtt cctgacaacg caattgacgc attgtacaca cacaattcat
      541 tttgagttgt tgtaatagca accgttggca atgcattttc agtcaccacc atcgtatctt
      601 ttccaacgca accatttgca tctgtaacaa taacagaata cgttcctgtt gttgtaactg
      661 ttaacgtttg gtttgttgag ttatcgttcc acaaataaga tgaaccagcg ttttgagcgt
      721 ctaatgtgct tgcataagta gtcccgtgac aataactatc atcagcacct aaacttacca
      781 caggcaatgc attttctgtt actacaattg tatcttttcc aacacaacca tttgcatctg
      841 ttacgataac agagtatgct ccagcagttg taactgttaa catttggttt gttgaattgt
      901 cattccacaa ataagttgaa ccagcgtttt gtgcatttaa tgtgtttgca taagtagttc
      961 catgacaata gctatcatct gcacctaaac ttaccactgg caatgcattt tctgtaacca
     1021 cgattgtatc ttttccaacg caaccgtttg catctgttac gataacagag tacgttcctg
     1081 tagttgtgat tgttaaagtt tgatttgttg agttatcatt ccataaataa gctgaaccag
     1141 cgttttgtgc atttaatgtg tttgcataag tagttccatg acaatagcta tcatctgcac
     1201 ctaaatttac aaccggcaat gcattttcag taactacgat tgtatctttt ccaacacaac
     1261 cgtttgcatc tgtaacaata acagaatacg ttccagctgc tgtaattgat aacatttgat
     1321 ttgttgagtt gtcgttccac aaataagatg aaccagcgtt ttgtgcgtct aatgtgcttg
     1381 cataagtagt tccgtgacaa taactatcat cagcacctaa atttacaacc ggcaatgcat
     1441 tttcagtaac tacgattgta tcttttccaa cgcaaccgtt tgcatcagta acaataacag
     1501 aatatgttcc agctgatgtg attgctaatg tttgatttgt tgagttgtca ttccacaaat
     1561 aagttgaact agcgttttga gcgtcaactg ctaaagatgt gcctgagcaa attgcagtat
     1621 cgactcctaa gtttacagtc aaatatgaag gtacttgtac ctcataagct cccatgtcga
     1681 ttgtgttttc gaataatctt ggatttcctg caagatctga atctgaaaat agtgttgagc
     1741 catataaagt attatttcct gcattcactg gcgcagaaca aggttttaat cgataatttt
     1801 cattcgccgc atccacaaac gggttggttg ttgatagaat atttcctcct ccatctgttc
     1861 cccaattggt agcattccaa gaaatacctt ctatcaaact attttttaca ataggagtac
     1921 tattctgtgc tgaaagcata actgcattat tagaagccgt atttccaaac agaatggaat
     1981 tattaattgc tggtgatgta taataataat tatacattcc accagcataa tatgctgaat
     2041 tatttgcgaa agtggtgttc gtaatggttg gtgatgaata ataataatta tacatcccgc
     2101 cacaagaata aatcgctgaa ttatttgcaa tggttgagtt ggtaatggtt ggtgatgaat
     2161 aatcattaaa catcccgcca ccatcagatg ctaaattatt tgtgatggtt gagttggtaa
     2221 tggttggtga agaagaagaa ttatacatcc cgccagcata atatgctgaa ttatttgcgg
     2281 cgatggtaga gttggtaatg gttggagatg aattttcgtt aaacatcccg ccaccattaa
     2341 aagctgaatt atttgcgatg gttgagttgg taatggttgg tga
//
LOCUS       DAOQVQ010000391         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_54890_length_2380_cov_4.043011, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000391 DAOQVQ010000000
VERSION     DAOQVQ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54890_length_2380_cov_4.043011"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKN22_10050"
     CDS             complement(<1..199)
                     /locus_tag="PKN22_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNS43089.1"
                     /translation="MERLEILKTYKIYIGGAFPRTESGRYYTPVNSKGEKLGNMCLSS
                     KKDVRNAVVAARKAFGGWSERA"
     gene            complement(202..1656)
                     /locus_tag="PKN22_10055"
     CDS             complement(202..1656)
                     /locus_tag="PKN22_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS43090.1"
                     /translation="MAKNKKTEELNWSYAPSLESTDHIQLQKKYELFIDGKFVKPKSG
                     NYFDTINPASGEVLASVANAGKEDVDKAVKAAKNAFKSWSKLSGKERGKYLYRIARLL
                     QERAREFAVIETLDAGKTIRESRDIDIPLAANHFFYYAGWADKLEYAFPNRKVEPIGV
                     AGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAESTPLTALKLAELLQDAGLPAGVVN
                     IVTGFGDTGEAIVRHQDVDKIAFTGSTPVGKKIQQIALETGKKCTLELGGKSANIILD
                     DAPIDQAVEGIVNGIFFNQGHVCCAGSRLLVQENIAATVIQKLKARIQTLIVGNPLDK
                     NTDIGAINSKKQLEIIEKYIQLGQAEGAEMYQPECTLPANGFYCKPTLFSNVAQSNRI
                     AQEEIFGPVLTIQTFRTVDEAIQKANNTQYGLAAGVWSDKGARLFNLTSKLNAGVVWG
                     NTYNKFDPTSPFGGFKESGFGREGGIHGLAAYIK"
     gene            complement(1671..>2380)
                     /gene="deoC"
                     /locus_tag="PKN22_10060"
     CDS             complement(1671..>2380)
                     /gene="deoC"
                     /locus_tag="PKN22_10060"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNS43091.1"
                     /translation="AAHLHDVYDDIPTVAAVCVYPSMVKIAREALGENSPIKIASVST
                     AFPSGQANWQTKETDTKLALQNGADEIDMVISRGKFLEGEYNFVFDEIAKMKELCGKA
                     RLKVILETGELVTLDNIRKASDIAIYAGADFIKTSTGKIQPAATMQTTYTMLMAIKDY
                     YDKTGIMVGMKPAGGISNAKLALQYLVLVHETVGAKWLNNEWFRFGASSLANDVLMQI
                     VKQKTGVYQSSDYFSID"
BASE COUNT          642 a          549 c          418 g          771 t
ORIGIN      
        1 ccgctctttc cgaccaacca ccaaatgctt ttcgggctgc aactactgca tttctaacat
       61 cttttttaga acttaggcac atgtttccca gcttctcccc ttttgagtta actggtgtgt
      121 aatatcttcc tgattccgtg cgtggaaatg ctccaccgat gtatatttta tatgttttta
      181 aaatttccaa tctttccatt tttacttaat gtatgctgct aaaccatgaa ttccgccttc
      241 tcttccaaag ccactttctt taaatccacc aaacggtgaa gtaggatcaa atttattgta
      301 agtattgccc cacaccacac ccgcattgag tttggatgta aggttgaaca aacgcgcgcc
      361 tttgtccgac catacaccag ccgctaaacc atattgcgtg ttattggctt tttgaatggc
      421 ttcgtcaacc gttctaaatg tttgaatggt caatacggga ccaaaaattt cttcttgagc
      481 aatgcggttg gattgcgcca catttgaaaa taaggttggt ttgcaataaa acccatttgc
      541 tggcaaagtg cattctggct gatacatttc tgcaccttct gcttgcccca attggatgta
      601 tttttcaatg atttccaatt gttttttaga gttgatagca ccaatatcgg tgtttttatc
      661 caacggattt ccaacaataa gtgtttgaat acgtgctttt aacttttgaa taaccgttgc
      721 ggcaatattt tcttgcacca acaaacgaga acccgcacaa caaacatgtc cttgattgaa
      781 gaaaatgccg tttacaattc cctctaccgc ttggtctatt ggagcatcgt ctaaaataat
      841 attagccgat ttaccgccta attccaacgt gcatttttta cccgtttcga gtgcaatttg
      901 ctgaattttc ttacccactg gcgtggagcc tgtaaaagca attttatcca catcctgatg
      961 gcgcacaata gcctctccag tatcaccaaa acctgtaaca atgttcacaa cacctgctgg
     1021 caaacccgca tcttgtagca attcagccaa tttcaatgcc gttaacggag ttgattctgc
     1081 tggttttaac acaaccgtat ttccacaagc aagcgctggt gctattttcc aagccgccat
     1141 gagtagtgga aaattccaag gaatgatttg tccagccacc ccaattggct ccacttttct
     1201 attgggaaaa gcatattcta acttatcggc ccaacctgca taatagaaaa aatgattggc
     1261 tgccaaagga atgtcgatat cacgcgattc tcgaatggtt tttcctgcat ccaaggtttc
     1321 gattacagca aattcacgtg cacgttcttg caacagacga gcaatgcggt acaaatattt
     1381 gcccctctcc ttgccagaaa gtttactcca cgatttaaac gcatttttag cagcttttac
     1441 agctttatcc acatcttcct tgcctgcatt tgcaaccgaa gctaaaacct cgccagaagc
     1501 aggatttatt gtgtcaaaat agttgcctga ttttggtttt acaaattttc catcaatgaa
     1561 taattcgtat tttttttgga gttgaatgtg gtcggtactt tccaacgagg gagcatagga
     1621 ccaattcaat tcttctgttt ttttattttt tgccattaca ctgaatgttt ttaatcgata
     1681 gagaaataat cacttgattg gtaaacgcct gttttttgtt tcacaatttg catcaacaca
     1741 tcgtttgcca agctactagc gccaaagcgg aaccattcgt tattgagcca ttttgcaccc
     1801 actgtttcgt gtaccaaaac caagtattgc aatgctaatt ttgcgttgga aataccgcct
     1861 gctggcttca ttcccaccat gatacctgtt ttatcgtaat aatctttaat tgccatcaac
     1921 atggtatagg ttgtttgcat ggtagcagct ggttgaattt ttccagtgga tgttttaata
     1981 aaatctgccc cggcataaat tgcaatatcg ctcgctttgc ggatgttatc caaggttacc
     2041 aattcacctg tttccaaaat aactttaagg cgtgcttttc cacacaattc tttcatttta
     2101 gcaatttcat caaacacaaa attgtattca ccctccaaga attttcctct ggaaatcacc
     2161 atatcaattt cgtcggctcc attttgaaga gccaatttag tatcggtttc tttagtctgc
     2221 cagttggctt gccccgaagg aaaagctgta gaaacagaag ctattttgat aggcgagttt
     2281 tctcccaatg cttcacgagc aattttcacc atcgaagggt acacacatac ggcagcaaca
     2341 gttggaatgt catcataaac atcgtgcaaa tgagcagctt
//
LOCUS       DAOQVQ010000392         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_54952_length_2379_cov_2.716867, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000392 DAOQVQ010000000
VERSION     DAOQVQ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54952_length_2379_cov_2.716867"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKN22_10065"
     CDS             <1..539
                     /locus_tag="PKN22_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43092.1"
                     /translation="VVNSGGLSYPNVDSTLSVIDPVALVETNKIVVGKNPYRISSDDN
                     GNLYIVTQTGFNTTNTQWVKVKPTLEIDTIYNWPVNLMTKIGSNFMLYTLTGSNQAAI
                     QLFNPTTQTITNTNFIDASLFTTFYGAQYDAQRNQIYCFDAMSYVNTGYVRVFSSNGV
                     HLKNYKTQLNPSKLIIYE"
     gene            532..1494
                     /locus_tag="PKN22_10070"
     CDS             532..1494
                     /locus_tag="PKN22_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43093.1"
                     /translation="MNKFFTILTLLPFGTLAQSYAPAAGQVGSTAIHKDSSIFIGWAT
                     GCVVERGYMNVEDQSLGYASFGTTAEALGVANGVTTSTVSLGDNGKATLTFDIPIGNG
                     PGPDFAVFENGLNDSFLELGFVEVSSDGTNFVRFPAISEIQTDTQIGGFGNVDCRYIH
                     NFAGKYRVGYGTPFDLDDLVGSPNLDLDNITHVRIVDVVGSIDPAFGTYDSQGTIVNN
                     LHPTPFESGGFDLDAVGIIHTSQEAGIEKLYHQLVAYPNPTKGNLQINVQVSDDFWVT
                     NTYGQTVISGTLKVGANQLDVSSLPAGIYFVQTLQGKTLKVVKE"
     gene            1561..>2379
                     /locus_tag="PKN22_10075"
     CDS             1561..>2379
                     /locus_tag="PKN22_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNS43094.1"
                     /translation="MLTPQIDKKLFLIDAYAIIFRSFFAFVKNPRINSKGLNTSAAFG
                     FTNHLLDVLKNEQPTHIAVVFDPPGGSTVRKESFEEYKANRNETPEDILSNIEPIKQI
                     IDAFNIPIIEIPGYEADDVIGTLSKIAEKNGFTTYMVTPDKDYGQLVTEKSMMFRPGR
                     GGEPAQIWGPAEVCKKFELNSVEQVIDYLGLMGDASDNIPGMPGVGPKTASKLLQEYG
                     TMENIFANTDKLKGKQKEIFETQQDLGRLSKELATIITDVDVDFDEHALELNAPD"
BASE COUNT          769 a          445 c          445 g          720 t
ORIGIN      
        1 atgttgtaaa ttcaggtggt ttaagctatc caaatgttga ttcaaccctt tctgtaattg
       61 accctgtggc gttagttgaa accaataaaa tagttgttgg aaaaaaccca taccgcattt
      121 cttcggatga caatggcaat ctttatattg tgactcaaac aggctttaac accaccaata
      181 cacaatgggt aaaagtaaaa ccaacccttg aaattgatac catttacaat tggcctgtaa
      241 atttgatgac caaaataggt tctaacttta tgttgtacac tctaaccggt tccaatcaag
      301 ctgccattca gctgttcaat cctacaacac aaaccataac caacaccaac ttcattgatg
      361 ccagcttatt taccacattt tatggtgctc aatacgatgc acaacgcaat caaatttatt
      421 gttttgatgc catgtcgtat gtcaacacag gatatgtgcg cgtattctcc agcaatggcg
      481 ttcatttaaa aaattacaaa actcaattaa atccttcaaa attaattatt tatgaataaa
      541 ttttttacaa ttttaaccct tttacctttt ggtacattag cacaaagtta tgctccagca
      601 gccgggcaag taggttcaac agctattcac aaagacagtt caatttttat tggttgggca
      661 actggttgtg tcgttgaacg aggttatatg aacgtagaag atcaatcctt gggatatgca
      721 tcttttggta caacagcaga ggcattggga gttgcaaatg gtgttactac atcaactgtt
      781 tcattaggcg ataatggcaa agcaacatta acctttgata ttccaattgg caatggtcca
      841 ggaccagatt ttgctgtttt tgaaaatgga cttaacgata gctttttaga acttggattt
      901 gtagaagtca gtagtgatgg aaccaatttc gttcgttttc cagctatttc agaaattcaa
      961 accgatacac aaattggagg atttggaaat gtagattgcc gatacattca caattttgca
     1021 ggaaaatacc gtgtaggata tggaactccg tttgatttgg acgatttagt aggttctccc
     1081 aacttagatt tagacaacat cacacacgtt cgtattgtgg atgtagtggg cagcatcgac
     1141 ccagcatttg gaacttacga cagtcaagga actattgtca acaatttgca ccctacccct
     1201 tttgaatcgg gtggttttga tttggatgcc gttggaatta tccataccag ccaagaagca
     1261 ggaattgaaa aattgtacca ccaattggtt gcctatccca accctacaaa aggaaatttg
     1321 caaatcaacg tgcaagtaag cgatgatttt tgggtaacaa acacctatgg tcaaaccgta
     1381 atttctggaa ccttaaaagt tggcgccaat cagttggatg tttcttcact tccagcagga
     1441 atttattttg ttcaaactct tcaaggcaaa actttaaaag tggttaaaga atagttgttg
     1501 cacatttttt tagacaataa accaagcaga atctgtatat ttggagtcta aatttgttca
     1561 atgcttacac ctcaaatcga caaaaagtta tttctaattg atgcttatgc aattattttt
     1621 cgttcgtttt ttgcgtttgt aaaaaatccg cgcatcaatt caaaagggtt aaacacttct
     1681 gctgcatttg gttttaccaa ccatttgttg gatgtattaa aaaacgagca accaacacac
     1741 attgccgtag tgtttgatcc tccaggcggt tctactgtca gaaaagaatc ttttgaagaa
     1801 tacaaggcaa atcgcaatga aacgcccgag gatattttat ccaacataga acccatcaaa
     1861 caaattattg atgcattcaa cattccaatt attgaaattc cgggatatga agccgatgat
     1921 gtgattggaa cactttcaaa aattgccgaa aaaaatgggt ttaccaccta catggttact
     1981 cctgataagg attatgggca attggtgacc gaaaaatcaa tgatgtttag acccggtcgt
     2041 ggtggcgaac ctgcacaaat ttggggacca gcagaagttt gcaaaaaatt tgaactcaat
     2101 tcagttgaac aagtaatcga ctaccttggt ttgatgggcg atgcatctga taacattcct
     2161 ggaatgccgg gtgttggacc aaaaactgct tctaaattgt tgcaagaata tggaacaatg
     2221 gaaaatattt ttgcaaatac cgataaatta aaaggcaagc aaaaagaaat ttttgaaacg
     2281 caacaagatt taggtcgtct ttctaaagaa ttagcaacca tcataaccga cgtagatgtt
     2341 gattttgatg agcatgcatt ggaattaaac gcacctgat
//
LOCUS       DAOQVQ010000393         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55192_length_2372_cov_3.773414, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000393 DAOQVQ010000000
VERSION     DAOQVQ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55192_length_2372_cov_3.773414"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..824)
                     /locus_tag="PKN22_10080"
     CDS             complement(3..824)
                     /locus_tag="PKN22_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS43095.1"
                     /translation="MKTKNFIVPHDFSKIADIALEHAIVTAKFVNAEINVLHVVAKGK
                     EIAEAEETLKTILAKFQTDIKLIPNVRVGNIFEDIGDFAAEKHAELIFMGTHGVQGWQ
                     HITGSKALKVVTNSNVPFIIVQEKGPKSTGYDNIVVPLDLNKETKQKLTVVAEIAQYF
                     KSKIHVITPEENDEFLRNQVKANILFANKFFSERNIEVSTTVVPSANFEDEIIKHAAM
                     VNGDLIAFMNLNKNNLLGVLSANHEIALMNNIAQIPTLIMNPIEGMSALAVDFNG"
     gene            complement(1034..>2372)
                     /locus_tag="PKN22_10085"
     CDS             complement(1034..>2372)
                     /locus_tag="PKN22_10085"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNS43096.1"
                     /translation="KNNSWQYWWTPNQYGAHTVEVKATASNGQVATETVNLNVSNAIS
                     DVVATTFNGDVIDMGTIGSQWFYGSYELPQFTGAYNSIIADFAISCPSVPGGCDDWDR
                     LAWIEVKAPNGDWMEIIRYITPYGVPCSQSIDLMDFASILQGKVDFRMYIETWGTGGW
                     KLDLDLNYKAGTPQYKYSSVEELWHGDYSFGDPANLQPTGSRNVAIESEVEAIWLRLV
                     STGHGWGSNNTGNAAEFYHATHHIHVDDISMYSQDLWQNCNPNPANCTGQMGTWQYNR
                     AGWCPGSMGMVFSYDLDSYLEEDTIKLDYIFQESYTDNCHPNNPNCVTGVTCSDCNAF
                     YNPYYRVGSYIIKYSNTPFLLSTETITKEEITANLYPNPTDGTFKLAIDSHWKNVVVS
                     IQNIAGDVVQTRFFATEQEMNAALFSIDQLASGIYFVKIQSEGQFTTMKVVKK"
BASE COUNT          739 a          459 c          357 g          817 t
ORIGIN      
        1 aattacccat taaaatcaac tgctaaagca gacattcctt caattgggtt cataattaat
       61 gttggaattt gagcaatgtt gttcatcaat gcaatttcat gattagcaga aagcacaccc
      121 aacaaattgt ttttgttcaa attcataaaa gcaattaaat caccgttaac cattgctgca
      181 tgtttgatga tttcgtcttc aaagtttgcg cttggaacaa cagttgtaga aacttcaatg
      241 tttctttcag agaaaaattt attggcaaat aaaatatttg cttttacttg gtttcttaaa
      301 aactcatcgt tttcttctgg agtgataacg tgaattttag atttaaagta ctgcgcaatt
      361 tctgcaacaa ccgtcaactt ttgtttggtt tctttattca aatccaatgg cacaacaatg
      421 ttatcgtaac ctgtagattt tggacctttc tcttgaacaa taataaaagg aacatttgaa
      481 tttgttacca ctttcaaggc tttacttcct gtaatgtgtt gccatccttg aactccatgg
      541 gttcccatga aaattagttc agcatgtttt tcagccgcaa aatcaccaat atcttcaaaa
      601 atatttccaa cacgcacgtt tggaattaac ttaatatctg tttgaaactt agctagaatt
      661 gtttttaggg tttcctctgc ttctgctatt tctttacctt ttgctaccac gtgcaacacg
      721 ttgatttcag catttacaaa ctttgcagtt acaatagcat gttctaatgc aatatcagca
      781 atctttgaga aatcatgagg tacaatgaag ttctttgttt tcatagaaaa ttattcaagg
      841 gttttatagt tttatacaaa tgtattaaaa aagtggttag ttgaagaaaa aaaataaaaa
      901 tatcgccaaa aaaactaaat attaatttga ttatttctca attcgttaat ctttaaaata
      961 aagaaaggcc atcccgaagg acagcctcgc tttttaccta actttactac tactttttaa
     1021 caaagatttt aaattatttt tttaccactt tcattgttgt aaattgacct tcactttgaa
     1081 tttttacaaa gtaaatacca cttgctaatt ggtcaattga aaacaatgca gcgttcattt
     1141 cttgttctgt tgcaaagaaa cgtgtttgta ccacatctcc agcaatgttt tgaatgctta
     1201 caacaacatt cttccagtgc gaatcaattg ctaatttaaa agttccatcc gttggatttg
     1261 gatataaatt ggctgtaatt tcttcttttg ttattgtttc tgttgacaat aaaaatggtg
     1321 tattgctgta cttaataatg taacttccta cacgataata tggattataa aaagcattac
     1381 aatcagaaca tgttacccct gttacacaat taggattatt agggtggcag ttatcggtgt
     1441 atgattcttg gaaaatataa tccaacttaa tggtgtcttc ttctaaataa gaatctaaat
     1501 catacgagaa aaccattccc atacttcctg gacaccatcc agctctgttg tattgccaag
     1561 ttcccatttg ccctgtacag tttgcagggt tagggttaca attttgccac aaatcttgtg
     1621 aatacatact aatgtcatct acatgaatat ggtgtgttgc atgataaaac tcagcagcat
     1681 tacctgtatt gttacttccc caaccatgtc ctgttgaaac caaacgcagc caaattgctt
     1741 ctacttctga ttcaattgca acgtttctag agcctgttgg ctgcaagttt gctggatctc
     1801 caaaactgta atctccgtgc cataattctt caaccgaaga gtatttgtat tgaggtgtgc
     1861 cggctttgta attcaaatct aaatccaatt tccatccacc tgttccccac gtctcaatgt
     1921 acattctaaa atctacttta ccttgcaaaa ttgaagcaaa atccatcaaa tcaattgact
     1981 gagaacaagg aacaccataa ggcgtaatgt aacgaataat ttccatccaa tcaccatttg
     2041 gagcttttac ctcaatccat gccaagcgat cccaatcatc gcaaccacct ggtacacttg
     2101 gacaagaaat tgcaaaatct gcaattattg aattgtatgc tcctgtaaat tgtggcaatt
     2161 cgtaagaacc ataaaaccat tgtgagccaa tagttcccat gtcaattaca tcgccattaa
     2221 aagtggtagc aacaacatca gaaatagcgt tgcttacatt taagtttact gtttctgtag
     2281 ctacttgccc attacttgcc gttgctttta cttcaactgt atgagctcca tattgattgg
     2341 gtgtccacca atattgccaa gaattatttt ta
//
LOCUS       DAOQVQ010000394         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55412_length_2367_cov_3.087803, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000394 DAOQVQ010000000
VERSION     DAOQVQ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55412_length_2367_cov_3.087803"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PKN22_10090"
     CDS             <1..983
                     /locus_tag="PKN22_10090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNS43097.1"
                     /translation="DDFFNSFNENDNRVSYIELPIYNSDNQLEWFGLYLTKLYSEETN
                     ELVEYLVVGRNITEKLKQDKLIATQQANITSSINYAQKIQLNLLPAVDKMKGCFSEQM
                     VYYKPKDIVSGDFYWCKQVDDYIIVAVGDGTGHGVPGAFMSILGINLLNSIVLEKNIY
                     DPGRILDELDIRLKQMLQEGKKQRIRDGIEITICAFNPINGTIDYSCAGSKILIHDGV
                     SISIRKGDSKHVGDEQPDFTNYITHHYEIAKETTIYMFTDGYYDQFGGFLQKKYTIRR
                     LLDLFTQNISLPLSEQREIVDTEFIDWKGENEQTDDITIVGLRILPPKNN"
     gene            992..1441
                     /locus_tag="PKN22_10095"
     CDS             992..1441
                     /locus_tag="PKN22_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43098.1"
                     /translation="MKISKLKTSFLIIQNWDGFASQLAVKDEFLNCFTENEHFEILLL
                     TNRKRPKENFEFAGRVHTISKSDFSLFGKLKSKNLLPSEHTHFDYILLLDDDFSALKK
                     IIQQMKIKNIVGFQSQQNYVNINLNTSVTKPTEKIIFALQTLSKFVI"
     gene            1443..2324
                     /gene="dapA"
                     /locus_tag="PKN22_10100"
     CDS             1443..2324
                     /gene="dapA"
                     /locus_tag="PKN22_10100"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNS43099.1"
                     /translation="MNNIFRGAGVALVTPFKDDKSIDFDALRKLVQLQINGNTDFLVV
                     QGTTGESPTLSWDEKIEILNTVIEENAGKLKIVFGVGGNNTLEVGKALQKVPKGVDGI
                     LSVSPYYNKPTQQGIIEHYKHIAACTELPIILYNVPGRTGSNMLPATIIELAKIPNIV
                     AVKEASGSMSQIMDIIRLAPAGFDVISGDDAITLPLIATGAVGVISVVNNALPEMFNA
                     MVHESLAGNFEVARKKHLRLLPITDMFFAEGNPGGVKVALETRGILKGNVRLPLMPVS
                     IALQEKIKAETLQLINA"
BASE COUNT          834 a          387 c          423 g          723 t
ORIGIN      
        1 ttgatgattt cttcaacagt tttaatgaaa atgacaatcg cgtttcatac attgagttac
       61 ccatttataa tagcgacaac caattggaat ggttcggttt ataccttaca aagctttatt
      121 ctgaagaaac caatgagttg gttgaatatt tagttgttgg tagaaatatt accgaaaaat
      181 taaaacaaga taaattaatc gccacgcaac aagcaaacat tacttcaagt ataaactatg
      241 ctcaaaaaat tcaactaaat ctattgcctg cagtagataa aatgaaagga tgttttagcg
      301 aacaaatggt ttattataag ccaaaagaca ttgtttcggg tgatttttat tggtgcaaac
      361 aagtggatga ctacatcatt gtggcagtag gtgacggaac agggcatggc gtacctgggg
      421 catttatgtc aattttaggt ataaacctac tcaattccat cgttttagaa aaaaatattt
      481 acgatcctgg aaggatttta gatgaattgg atattcgctt aaaacaaatg cttcaagaag
      541 gaaaaaaaca gcgaattaga gatggaattg aaataaccat ttgtgctttc aatccaatca
      601 atggtacgat tgattattct tgcgcaggct ccaaaattct tattcatgat ggtgtttcaa
      661 ttagcattcg caaaggagat agcaagcatg ttggcgatga acaacctgat tttaccaact
      721 acattacgca ccattatgaa attgctaaag aaacaaccat ttatatgttt actgatggtt
      781 attacgacca gtttggtggc ttcttacaga aaaaatatac aattcgacgc ttattagatt
      841 tatttactca aaacatcagt cttcctttgt cggaacaaag agaaattgtt gacactgaat
      901 ttatagattg gaaaggagaa aacgagcaaa cagatgatat aactatagtc ggactaagaa
      961 ttttacctcc aaaaaacaac tgattttaaa catgaaaatt agtaaactaa aaacttcttt
     1021 tttaatcatt caaaattggg atggatttgc atcacaactg gctgtaaaag atgaattttt
     1081 aaactgtttt acagaaaatg aacattttga aattttactg ctaaccaaca gaaaacgacc
     1141 gaaagaaaat tttgaatttg ccggacgtgt gcacaccatt tcaaaatccg acttttcact
     1201 atttggtaaa ttaaaatcaa agaatttatt gcccagtgaa cacacccatt ttgattacat
     1261 ccttctgctt gacgatgatt tttctgcatt aaaaaaaatc attcaacaaa tgaaaatcaa
     1321 aaacatagta ggctttcaaa gtcaacaaaa ttatgtgaat ataaacctta acacatctgt
     1381 tacaaaacca acagaaaaga ttatatttgc actacaaaca ctttcaaaat ttgtaattta
     1441 aaatgaataa tatttttaga ggagctggtg ttgctttggt tacacctttc aaagatgata
     1501 aatcaattga ctttgatgca ttgcgcaagt tggttcaact gcaaattaat ggcaacaccg
     1561 attttttggt tgtgcaaggt acaacaggcg aatctcctac cctttcttgg gatgaaaaaa
     1621 ttgaaatttt aaatactgtt attgaagaaa atgccggaaa attaaaaatc gttttcggtg
     1681 ttggtggaaa caatacgttg gaagttggaa aagcattgca aaaagttcca aaaggggtag
     1741 atggaatttt atccgtttct ccctactaca acaagccaac gcaacaagga ataattgaac
     1801 attacaaaca cattgcagct tgcaccgagt tgccaataat tttatacaac gtgcccggaa
     1861 gaacgggttc taacatgctt cctgcaacaa taattgaatt agcaaaaatt cctaacatcg
     1921 ttgctgtaaa agaagcttct ggtagcatga gtcaaattat ggacattatt cgccttgctc
     1981 ctgctggttt tgatgttatt tctggcgatg atgcaattac attgcctttg attgcaactg
     2041 gtgccgtagg cgttatttct gtagttaaca acgctttgcc agaaatgttc aacgcaatgg
     2101 ttcacgaatc tttggcaggt aattttgaag ttgctcgcaa aaaacacttg cgcttattgc
     2161 caattacaga tatgttcttt gcagaaggaa accctggtgg tgttaaagtg gcacttgaaa
     2221 caagaggaat ccttaaagga aacgtacgtt taccattgat gcctgtttcg attgcattgc
     2281 aagaaaaaat taaagcagaa acgttgcaac ttataaatgc ataagcaaat acttttcata
     2341 ggagttttag ttgttggagg aattaat
//
LOCUS       DAOQVQ010000395         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55486_length_2365_cov_3.059307, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000395 DAOQVQ010000000
VERSION     DAOQVQ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55486_length_2365_cov_3.059307"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PKN22_10105"
     CDS             complement(<1..939)
                     /locus_tag="PKN22_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43100.1"
                     /translation="MKYVFFILFWVVLFPLNAQKTIVLEEVVTDTLHIIQTPTTQNLT
                     IKTLNEFFEVYTNSTSLPGQKYPTQLQQRQLNEFVAYSKSINEKGLDYNLSAFMASSF
                     DLSKKYLLDKARAIDSTNYFVLTQAIGVNVILSNQNGIKKDLTTLVNQKKWTTDELNY
                     AAMTLESLPQNSVLITHGVNDSYPILYQQYVKNRRKDVVVIPMHLLVSKQYRNRLEES
                     AFQLPPNDLINVAYIQKMCALNDNKPIFLALTLSTSYLKPLEANLYPVGLSYHFSKIP
                     YQNSSKNEQLWKAHLKNSLYQLNDEEGKKLAANYLPL"
     gene            complement(986..1723)
                     /locus_tag="PKN22_10110"
     CDS             complement(986..1723)
                     /locus_tag="PKN22_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNS43101.1"
                     /translation="MKLELCAASVRALHLAKDLQLDRIELCENLMQGGITPSAGRIEF
                     ALNLGLETHLLVRPRPGGFNYSEDELKIMVSDIKNAKKMGVHGVVVGALNNNNTVNEE
                     ALKMFIDAAEGIEFTFHRAFDDGVFDWKKRLDVLINAGVTRVLTSGIARNVSAGMEVL
                     KEMKVYAGDKIQIMPGGGISAGNVGQLIKLVQPEAIHFSGTVKTVVDEDSNFSETFLA
                     IDEERVNRILTEVRVALDKLNNTPRMV"
     gene            complement(1746..>2365)
                     /locus_tag="PKN22_10115"
     CDS             complement(1746..>2365)
                     /locus_tag="PKN22_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43102.1"
                     /translation="NKKGIEISVMPNGNINTILPVYKDSVDFKRSNDALQSYTITAFE
                     NRLNTNSKTQFYKHKGLGLPIEFITQPHPSYAKNAKAMLVDGFKGIHQFSNNDWLGFN
                     EGIVKFIVNLDKKINYKKVTVGMLTDVSSWIHLPKEMKVFASKNGKKWKEIANTKINS
                     EATTVELGGKIKQLKFEIIPMDVIPEGFGGAGYQPFTFLDELIFE"
BASE COUNT          701 a          426 c          420 g          818 t
ORIGIN      
        1 caatggcaag taattagctg caagtttttt gccttcttcg tcatttaatt ggtaaagact
       61 attttttaag tgagctttcc acaactgttc gtttttactt gaattttgat aaggaatttt
      121 tgaaaaatga tagctcaaac caacaggata taaattggct tccaagggtt ttaaatagga
      181 agttgaaagc gttaatgcta agaaaatagg tttgttatca ttgagcgcac acattttttg
      241 gatgtatgca acgttgatta aatcgtttgg gggcaactga aaagcacttt cttctaaccg
      301 gttgcggtat tgtttactca ctaacaaatg catgggaatg accaccacat ctttgcgtct
      361 atttttcaca tattgttggt acaaaatagg gtaactatcg tttacaccgt gagttattaa
      421 aacactattt tggggtaaac tctccaaggt catagcggca taattgagtt catctgtggt
      481 ccattttttt tgatttacca atgttgttag gtcttttttt atcccatttt ggttagacaa
      541 aataacgtta acaccaatgg cttgagtgag tacaaaatag ttggtagaat caatggcgcg
      601 tgccttatcg agcaagtatt tttttgataa atcaaaggag gaagccataa aagcgctcaa
      661 gttgtaatcc aatccttttt cgttgatgga tttgctgtat gctacaaatt cattgagctg
      721 acgttgttgc aattgcgttg gatatttttg tccaggtagt gaagtggaat tggtataaac
      781 ttcaaaaaac tcgttgaggg tttttattgt taaattttga gtcgtgggtg tttgaattat
      841 gtgaagagtg tcagtgacaa cttcttctaa aacaatcgtt ttttgcgcat ttaatggaaa
      901 aagcacaacc caaaatagta taaaaaaaac gtatttcatt tgattttttt ttaagctgta
      961 caaataacaa atgaaatggt tcaaattaaa ccatacgagg tgtgttattt aatttatcaa
     1021 gggccactct cacctctgta agaatgcggt tgacacgttc ttcgtcaatt gctaaaaacg
     1081 tttcagaaaa attagaatct tcgtccacaa cagttttgac tgttccagag aaatgaatag
     1141 cttcgggttg caccaatttg attagttgtc ccacatttcc agcacttatt ccgccaccgg
     1201 gcatgatttg aattttatca cctgcataga ctttcatttc ttttagcact tccattccag
     1261 cagaaacatt gcgagcaatt ccagatgtta aaacacgagt tacaccagca tttatcaata
     1321 catccaaacg ttttttccag tcaaaaacac catcgtcaaa agcacggtga aaagtaaatt
     1381 ctattccttc cgcagcatcg ataaacattt tcaaggcctc ttcattgaca gtattgttgt
     1441 tgttcaacgc accaactaca acgccgtgta cgcccatttt tttagcattt ttaatatcgg
     1501 ataccatgat ttttaactca tcctcagagt agttgaaccc acccggacga gggcgcacca
     1561 acaaatgcgt ttctaaccct aaattcaacg caaattctat tctgccagca gagggtgtaa
     1621 ttccgccttg catcaggttt tcacataatt ctatacgatc cagttgaaga tcttttgcta
     1681 aatgaagtgc tctaacagaa gcagcgcaca attcaagttt catattcttt tgttttagcg
     1741 agttattatt caaaaatcaa ttcgtctaaa aaagtaaatg gttggtatcc tgcaccacca
     1801 aaaccttcag gaattacgtc cattggaatg atttcaaatt ttaattgctt gattttccct
     1861 cccaattcaa cggttgttgc ttctgaatta attttagtgt tggcaatttc tttccatttt
     1921 ttaccatttt ttgatgcaaa aactttcatt tctttaggta aatgaatcca cgaagaaacg
     1981 tcggttagca taccaacggt tactttttta tagttaattt ttttgtctaa gttaacaata
     2041 aatttgacaa ttccctcatt aaatcccaac caatcgttgt tgctaaattg atgaatacct
     2101 ttgaaaccat ccaccaacat agcttttgca tttttagcat agctagggtg gggctgtgtg
     2161 ataaattcaa taggtaatcc caatcctttg tgtttgtaga attgagtttt tgaattggta
     2221 tttaaacgat tttcaaatgc agtgattgta taggattgca aagcatcgtt gctgcgttta
     2281 aaatctaccg aatctttgta aacgggtaaa atggtgttga tatttccatt gggcattacc
     2341 gaaatttcaa tgcctttttt gttgg
//
LOCUS       DAOQVQ010000396         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55520_length_2364_cov_3.560416, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000396 DAOQVQ010000000
VERSION     DAOQVQ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55520_length_2364_cov_3.560416"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKN22_10120"
     CDS             complement(<1..729)
                     /locus_tag="PKN22_10120"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS43103.1"
                     /translation="MKIVIIEDEDLLAEDLSDILKEIDNSIEIVKHISSVKEGVQFFN
                     STEETIDLIFSDIQLGDGLSFDLFKATQLNVPVIFCTAYNQYAINAFKANGIEYILKP
                     FSTKTITEALERYKNLKNNFTENIRKVEQQLQPVENQNNTLVVHHKDKIIPLNREDIA
                     LVYIRNEQVTIRNFEGEQYYINKSLDELDQLLGDQFYRANRQFIVNKNAVKDITTLIN
                     RKYLVNLNFEFKQQIVVSKEKMSSF"
     gene            complement(710..1777)
                     /locus_tag="PKN22_10125"
     CDS             complement(710..1777)
                     /locus_tag="PKN22_10125"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS43104.1"
                     /translation="MQYQIPKEDLRKYSMKFSLLIMLIAFVSIYVMEKNSHIIFYRTF
                     MAFLHFYFCFRLNILLYSVFSKKNKEINERKKVTIGIGLTAIFILITSYTTQFLIYKG
                     IFPSDLNDSRAIQIFYTWRAFVFIPFLAIVTFSTAHFFHRSVILMHATRQSELEVSRL
                     QTINSETLNQLLKQQIQPHFLFNALNTLKSLIKKHPDVAENYLMQLSDFLRASISEHR
                     SDVVSLKEELALCENYMNMQKTRFGDALQYSNLIEEEGLEQLFVPFFSIQPLLENAIK
                     HNELTKENPLLIKVFIENDVIVVENNLQKRKYVENSTGNGLKNLGERYRLLKEKDIEI
                     IENEDKFVVRLKLISNENSNH"
     gene            1918..2208
                     /locus_tag="PKN22_10130"
     CDS             1918..2208
                     /locus_tag="PKN22_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43105.1"
                     /translation="MNKKALLITYYWPPAGGPGVQRWLKIVKYLPEYNIIPIVLTVDE
                     FYASYPLIDTTLSNDIHSNTLVYKTKSFEILDFYKKLNKKKRPISFSGLFYK"
BASE COUNT          798 a          367 c          361 g          838 t
ORIGIN      
        1 aaaagacgac attttttctt tactcacaac tatttgctgt ttaaactcaa aatttaggtt
       61 caccaaatac ttacggttta tcagtgttgt aatatctttc accgcatttt tattgacaat
      121 aaactgacga ttggcacggt aaaactgatc tcccaaaagt tgatctagtt catccaatga
      181 tttgttgata taatattgtt ctccttcaaa attacgtata gtaacttgtt cgtttctaat
      241 ataaaccaaa gctatatctt ctctgttgag cggaataatt ttgtctttgt ggtgtacaac
      301 caacgtgtta ttttgatttt ctacaggttg taattgttgc tctacttttc taatgttttc
      361 tgtaaaatta ttttttaagt ttttataacg ttccaatgcc tcagtaatgg tttttgttga
      421 aaacggtttg agaatgtatt ctatcccatt ggctttgaaa gcattgatgg cgtattggtt
      481 gtaggcggtg caaaaaataa ctggaacgtt caactgggta gctttaaata aatcaaaact
      541 caatccatct cccaattgaa tatcgctaaa aatcaagtcg atagtttctt cggttgaatt
      601 aaaaaactga acaccttctt taacactaga aatgtgtttt acaatttcaa ttgaattatc
      661 aatttctttt aaaatgtccg ataaatcttc ggccaaaaga tcttcgtcct caatgattac
      721 tattttcatt tgaaataagt tttaatcgaa caacaaattt atcttcattt tcaataattt
      781 caatgtcctt ttctttcagc aagcgataac gttcacccaa gtttttcaat ccatttccag
      841 tagaattttc tacatatttt cttttttgga ggttattttc taccacaatt acatcatttt
      901 caatgaatac tttgattaga agtggatttt ctttggtgag ttcattgtgt tttatagcat
      961 tttctaacaa gggttgaatg gagaaaaaag gcacaaacaa ttgctccaaa ccttcttctt
     1021 ctattaaatt ggaatattgc aaagcatcgc caaaacgtgt tttttgcatg ttcatgtagt
     1081 tctcacacaa agccaattct tcttttaatg atacaacatc tgagcggtgt tctgaaatgg
     1141 aggctcgtaa aaaatcggat aattgcatta aatagttttc tgcaacatct ggatgttttt
     1201 taatcaacga tttgagggta ttcaacgcat taaacaagaa gtgcggttga atttgctgct
     1261 tcaataattg gtttaatgtt tctgagttaa ttgtttgtaa acgtgaaact tccaattccg
     1321 attgtctggt tgcatgcatt aaaatcacag aacggtgaaa aaaatgtgcc gttgaaaaag
     1381 ttacgatggc taaaaatggg ataaacacaa atgccctcca ggtgtaaaat atttgaattg
     1441 cacggctatc attcaaatca ctagggaaaa ttcctttgta aattaaaaat tgagtggtat
     1501 aagaagtaat tagtataaaa atggcagtta aaccgatacc aatagttact tttttacgtt
     1561 cgtttatttc tttatttttt tttgaaaata cagagtacaa aagaatattt aaccgaaaac
     1621 aaaagtaaaa atgcaaaaaa gccataaaag ttcggtagaa aataatgtgc gagtttttct
     1681 ccatcacata gatgctcaca aaggcaatga gcataatcag caatgaaaat ttcatgctat
     1741 attttcgaag gtcttctttg ggtatttggt attgcattta acaaaagtta ttatgtaaaa
     1801 ctcaaaaaat attttgatgt ataaaattag aataaaaaat ccaaacagca gtaattgctt
     1861 caattcattc aaaaataaaa cttcaacttt caatttttag attaaatttg ttcacaaatg
     1921 aataaaaaag cacttctgat aacgtattat tggccaccag ctggaggtcc aggtgtgcaa
     1981 cgttggttaa aaatagtaaa gtatttacca gagtataata ttattccaat tgttttaaca
     2041 gttgacgagt tttatgcaag ttacccatta attgacacaa ctttgagtaa tgatattcat
     2101 tcaaatacgt tggtttacaa aacaaaatct tttgaaatat tagattttta caaaaaatta
     2161 aataaaaaaa agagaccaat atcattttcc ggtctctttt ataaataatt taatcgattt
     2221 tcaagctttc aaagcatttt caaagaaaaa ttctgcatct tttcggaatt tttccaacga
     2281 tggcttatgg atatacatca tatgccccgc ttcataataa gtgagactca ggtttggctg
     2341 caaaacagga tcaagctgta aatg
//
LOCUS       DAOQVQ010000397         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55681_length_2360_cov_2.940130, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000397 DAOQVQ010000000
VERSION     DAOQVQ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55681_length_2360_cov_2.940130"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKN22_10135"
     CDS             complement(<1..467)
                     /locus_tag="PKN22_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43106.1"
                     /translation="MVNIFIANLDWSITSEDLKTTFSSFGEVSYAHVVFEKDTKRSCG
                     YGYVEMEDPTAAITAIGALNGLEINGRAIDVKIASPKTNRPKKVEKPQEKKSFKKKPF
                     GQKRGGQGGYNRDNRGGDRPSRYNRDRSEGSGERQGGYNNQRSEGGYSAPRNS"
     gene            complement(500..1450)
                     /locus_tag="PKN22_10140"
     CDS             complement(500..1450)
                     /locus_tag="PKN22_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43107.1"
                     /translation="MSEVEQPKKNGLNTLLLIVLLAGIGVMTYLWSSTRTELSESNSQ
                     LETMSQMLSQYTGEISNDLSADLKNMLATYDALEKKAKEQGDLNEQQQAEIEEQKNQI
                     KDLMEKVKQGKWTAAELAKMRRENETLRNIMKGYVYQIDSLNTLNLKLTSDLDQTKTE
                     LTNTTAERDNFKQEAEASNARVKEGSKLQAYGFSSVALRSKLNNTTTETDKAKNTIQI
                     KSSFTIGANPIATKGTKPVYMQVTKPDGTIFQNRASNILSTEQGSIAYSDKKDVDYTG
                     EAVSMAIYYDLRGEEAQKGNYTVKIYCDGNLIGKDNFTLK"
     gene            complement(1534..>2360)
                     /locus_tag="PKN22_10145"
     CDS             complement(1534..>2360)
                     /locus_tag="PKN22_10145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686020.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNS43108.1"
                     /translation="VWSEDEIQGAWDSARQEAGAAFGNDGIYMEKYIEEPRHIEIQIA
                     GDQYGNACHLSERDCSIQRRHQKLVEETPSPYMTDELRERMGQAAIKACKAVNYEGVG
                     TIEFLVDKNRDFYFMEMNTRIQVEHTITEEVINYDLIKEQIKIAAGEKISGKNYYPQM
                     HAIQCRINAEDPFNDFRPSPGKITTYHAPGGLGVRIDTHVYSGYTIPSNYDSMISKLI
                     VVAQSREEAILKMKRALDEYVIEGVKTTIPFHQKLMDNEDFRKGEFTTKFMETFKF"
BASE COUNT          644 a          480 c          351 g          885 t
ORIGIN      
        1 tcactatttc ttggagcact gtaaccacct tcactacgtt ggttgttata tccaccttgg
       61 cgttcaccac ttccttcaga gcgatctctg ttgtatctgc taggacgatc acctcctctg
      121 ttgtcacggt tatagcctcc ttgacctccg cgtttttgac caaacggttt cttcttgaaa
      181 gacttctttt cttgaggttt ttccactttt ttagggcggt ttgttttagg tgatgcaatt
      241 tttacgtcaa ttgcacgacc attaatttca agtccattca aggctccaat tgcagtaatt
      301 gcagcagtag gatcttccat ttcaacataa ccatagccac atgatctttt tgtatctttt
      361 tcaaatacaa catgggcgta actaacttca ccaaacgacg aaaaggtagt ttttaaatct
      421 tcagaggtga ttgaccaatc aagatttgca ataaaaatat taaccatcta aactaaatta
      481 tatataccta aaaatctatt tatttcaacg tgaaattatc tttaccaatt aaattaccat
      541 cgcagtaaat tttaacggta taatttcctt tttgagcttc ttcacctctc aaatcgtaat
      601 aaattgccat gcttacagcc tcacctgtat aatccacatc ttttttatct gaataagcaa
      661 tacttccttg ttcagtagat aaaatattag atgctctatt ttggaaaata gttccatcag
      721 gttttgttac ttgcatataa acaggttttg ttccttttgt ggcaatagga ttcgcaccaa
      781 ttgtaaaaga agacttaatt tgaattgtat tttttgcctt gtctgtttct gttgttgtat
      841 tgttcaattt tgaacgtaac gctacagaag aaaaaccata ggcttgcaat ttacttcctt
      901 ctttaacacg tgcatttgat gcttctgcct cttgtttaaa attgtcgcgt tctgctgttg
      961 tattggtcaa ctctgtttta gtttgatcca aatcagaagt caatttcaaa ttcaacgtgt
     1021 tcaatgaatc aatttgataa acataacctt tcataatgtt tctcaatgtt tcattttctc
     1081 tacgcatttt tgccaattca gcagccgtcc acttgccttg ctttactttt tccatcaaat
     1141 ctttgatttg atttttttgt tcttcaatct ccgcttgttg ttgctcgttc aaatcacctt
     1201 gttcttttgc ttttttctcc aaagcatcat aagttgccaa catgtttttt aaatctgctg
     1261 acaaatcatt ggaaatctca cctgtatatt gtgataacat ttgtgacatg gtttccaatt
     1321 gtgaattgct ttcactcaac tctgtacgtg ttgacgacca taaataagtc attactccga
     1381 ttccggctaa taaaacgatc aataacaacg tgttaagacc atttttcttt ggttgttcta
     1441 cttctgacat aatctaaatt attaaacgtt ttatactaca aaaatacata attcaatata
     1501 aaacgaaaaa aataatattt atttgatacc taattaaaat ttaaatgttt ccatgaattt
     1561 tgttgtgaac tccccttttc taaagtcttc gttatccatt aatttttggt ggaatggaat
     1621 ggttgttttt acaccttcaa ttacatattc atccaacgct cttttcattt tcaagatcgc
     1681 ctcttcacgt gattgagcta caacgattaa tttagaaatc atcgagtcgt agtttgacgg
     1741 aatggtatat cctgaataaa catgtgtatc aattctaaca cctaatcctc ctggtgcgtg
     1801 atacgttgtg atttttcctg gtgaaggtct aaaatcatta aacggatctt ctgcattgat
     1861 acgacattga atagcgtgca tttgagggta ataatttttt cctgatattt tttcccctgc
     1921 agcaatttta atttgctctt taatcaagtc gtaattgata acttcttctg taatggtatg
     1981 ctcaacttga atacgtgtgt tcatttccat gaaataaaaa tcacggtttt tgtccaccaa
     2041 gaattcaatg gttcccactc cttcgtaatt caccgcttta caagctttaa ttgctgcttg
     2101 ccccatgcgc tctctcaatt cgtctgtcat atatggagat ggtgtttctt ccactaattt
     2161 ttggtgacga cgttgaatag aacaatctct ttcagacaag tgacatgcat taccgtattg
     2221 gtctccagca atttgaattt caatgtggcg tggttcctca atgtattttt ccatataaat
     2281 accatcgttt ccaaaagcgg ctcctgcttc ttgacgtgca gaatcccatg cgccttgaat
     2341 ctcatcttcc gaccaaacaa
//
LOCUS       DAOQVQ010000398         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55752_length_2358_cov_3.719062, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000398 DAOQVQ010000000
VERSION     DAOQVQ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55752_length_2358_cov_3.719062"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /gene="sprA"
                     /locus_tag="PKN22_10150"
     CDS             <1..2228
                     /gene="sprA"
                     /locus_tag="PKN22_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNS43109.1"
                     /translation="LALIREFNLNYVPKNFTFTNDLLRSYNERQVKNNLVPNYEFRPV
                     YIKQFTWNRSYNLGYDITRNLKLTFTANNRAIFAEPDGQVDKKESPDLYRDFKDSIRT
                     QMRTLGKTIDYTHNYNINYSLPLNKIPALDWTTATFTYGGSFNWQRAPLAQPEYGHTV
                     QNNRVINAQTQLNFTNLYNKLPFFKKVLSGGSKSTVGSARQRITTDGKNITVNNPQKP
                     AASKKPPVEERYPKPVPPKPEEEMTEKEKAKWDKKIERWEKKIERIKKREEKQKDKVN
                     PVAGFFARVLMTVRNVGATYSQNDGTLLPGYDEETRILGFGNDFKSPMAGFIFGKQRY
                     SVTGKETGFDFARVASEKGWLVKNENINRQYTNTHTQNITIRATLEPLKDLVIDLNAN
                     RTYGNSSTEFFRWNETSGLYEAQSRVDIAQLTYTNISIGSAFASLGKNYSSEVFKKLL
                     DNRAEVSALIGQKNSNSTQLSSGYYSGYSGTQQEVLLGAFLTSYTNRKVNDKNINPIK
                     NMPLPNWSINYNGLTKMEFMKKAVKSFVIRHAYTSTVSVAGMQTNLNATFDANDNPTA
                     LDINNNYIGRMQIQNVTMSERFSPLIGFDATWNVKKQGLITKFEISKERSSTLSLNNN
                     QVTEVLGTTYTIGTGYKFTNVRLPIKGLKPSDMNFRFDFSLRDNLTVIRKVVEQTDQA
                     TAGQKVMSIKASLDYNIGRFVILQLYYDQVVNKPKIATSYPTGNMSCGIKIRYNLAGV
                     Q"
BASE COUNT          860 a          390 c          439 g          669 t
ORIGIN      
        1 ggttggcatt gattcgtgag tttaatttga attacgttcc aaaaaacttt acgttcacca
       61 acgatttgtt gagaagttac aacgaacgtc aagttaaaaa caacctcgtt cctaattacg
      121 agttcagacc tgtttatatc aaacaattta catggaacag aagttataac ttaggatacg
      181 acatcacacg aaatttgaaa ttaacgttta cggctaataa ccgtgcaatt tttgctgaac
      241 cagatggtca ggtggataaa aaagaaagtc ctgatttata ccgtgatttc aaagactcta
      301 tccgcaccca aatgcgcact ttgggtaaaa cgattgatta tacccacaat tacaacatca
      361 attattcgtt gcctttaaat aaaattccag cattggattg gacaacggca acctttactt
      421 atggtgggtc ttttaactgg caacgtgctc ctttggcaca accagaatat ggacatacgg
      481 tacaaaacaa ccgtgttatc aatgctcaaa cacagttgaa ttttaccaac ttgtataata
      541 aattgccttt cttcaaaaaa gtattgagcg gtggaagtaa aagtacggtt ggaagtgcgc
      601 gtcaacgtat tactacagat gggaaaaaca tcacagttaa caatcctcaa aaaccagcag
      661 caagtaagaa accgccagta gaggagcgtt atccaaaacc tgtaccgcca aaaccagaag
      721 aggaaatgac cgaaaaggaa aaggcaaaat gggataagaa aattgaacgt tgggaaaaga
      781 aaattgaacg tatcaaaaaa cgtgaagaaa aacaaaaaga caaagtcaat ccagttgcag
      841 gattctttgc acgagtattg atgacggtgc gaaatgtagg cgcaacttat tctcaaaacg
      901 atggtacttt attgcctggt tacgatgaag aaacacgtat acttggtttt ggaaatgatt
      961 ttaaatcgcc aatggccgga tttatttttg gaaaacaacg ctatagtgtt acaggtaaag
     1021 aaacaggttt tgattttgcg cgtgttgcat ccgaaaaagg atggttggtt aaaaatgaaa
     1081 acattaatcg tcaatacacc aacacacata cgcaaaacat tactattcgt gccacattag
     1141 agccgttaaa agatttagta attgatttga atgctaaccg tacttatggc aatagttcaa
     1201 cagaattctt tagatggaat gaaacttctg gtttgtatga agcacaaagt agagttgata
     1261 ttgcacagtt gacttatacc aacatttcta tcggaagtgc atttgcatca ttgggtaaaa
     1321 actatagctc cgaggtgttt aaaaaattat tggataaccg tgcagaagtt tcggcattga
     1381 ttggtcagaa aaattcaaat tctactcaat taagcagtgg ttattattcg gggtacagcg
     1441 gaacacaaca agaagtgttg ttgggagcat tcttaacttc ttataccaat agaaaagtga
     1501 atgacaaaaa cattaaccca attaaaaaca tgccacttcc aaactggtca atcaactata
     1561 acggtttgac gaaaatggaa ttcatgaaaa aagcggttaa atcattcgtt attcgccatg
     1621 catatacttc aacggtttct gttgcaggaa tgcaaaccaa tttgaatgca acgtttgatg
     1681 caaatgataa tccaacagca ttggacatca ataacaatta cattggcaga atgcaaattc
     1741 aaaacgtaac catgtcagaa cgttttagtc cgttaattgg ttttgatgca acatggaatg
     1801 tgaaaaaaca agggttgatt acgaagtttg aaatttctaa agaaagaagt tcaacactat
     1861 cgttaaacaa caaccaagtt acagaagtgt tgggtacaac ttataccatc ggtacgggat
     1921 ataaattcac caatgttcga ttaccaatta aaggattgaa accaagcgat atgaatttca
     1981 gatttgattt ctcattacgc gataatttaa ccgtaattcg taaagtagtt gaacaaaccg
     2041 atcaagcaac tgcagggcaa aaagtaatgt caatcaaagc ttcattggat tacaatattg
     2101 gacgttttgt gatattacaa ttgtattacg atcaagttgt taacaaacca aaaatagcaa
     2161 cttcctatcc aactggcaat atgagttgtg gaattaaaat acgttacaat ttggctgggg
     2221 tacagtagaa tttagttttt ataccgttca atcaataaaa tgtgttaaaa atattgaaag
     2281 aagttttgtg tcttaatttt ttgctaaatt tgttaaatct ataaaattat aaaaatgaat
     2341 aaaaaattac tttttgga
//
LOCUS       DAOQVQ010000399         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55828_length_2356_cov_3.737071, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000399 DAOQVQ010000000
VERSION     DAOQVQ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55828_length_2356_cov_3.737071"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1261)
                     /locus_tag="PKN22_10155"
     CDS             complement(191..1261)
                     /locus_tag="PKN22_10155"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNS43110.1"
                     /translation="MIKEPITVKITPTSQTKIHQVDWNNLPFGKVFTDHMLVMDYTDG
                     KWQTPEIVPFGPIPMHPAMSALHYGQTIFEGMKAFRRENGDVVLFRPEMNAKRFAESS
                     ERLCIPAMEEEVFVELIRKLVEVDKEWVPKNEGYSLYIRPFVFATDEAIGVKPSDNYK
                     FIVFLCPVGAYYSKPVAVKVEEYYTRAAIGGVGRAKTAGNYASALYPALLSQKEGYSQ
                     LLWTDAKEHKYFEESGTMNICFVINDTLITPAEDEDTILRGTTKRTIVDVAQKWGYPV
                     EERKVSVEEIVKAAEEGRLQEAFGAGTAATIASITRINFRGKDFVLPEVAETNFSQKV
                     KTYLDGVKSGKFTDEDNWCLVV"
     gene            1367..>2356
                     /locus_tag="PKN22_10160"
     CDS             1367..>2356
                     /locus_tag="PKN22_10160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNS43111.1"
                     /translation="MSKSLETLQKYWGYNNFRPGQEAIIDDVINGHDVLALMPTGGGK
                     SICFQVPGLIREGLTLVISPLIALMQDQVTALDKKHILAKAITSGMSFRELDITLDNA
                     RFNGYQFLYTSPERIQSTLFIERFKLMNIGLIVIDEAHCISEWGHDFRPSFMEIKKLR
                     EIHPSVPIIALTATATPKTQQEIIERLELKNPKIHQSSFERKNLSYKSYLTNNKLQEI
                     IDTCKQFEGKTGIVYCQTRKSVKFVAQKLYSQQLSVGIYHGGMNRDERKKMLEDWLSN
                     KIKIIVATNAFGMGIDKPDVRFVLHYEFPNSLEAYFQEAGRGGRDGNESVAINY"
BASE COUNT          740 a          478 c          399 g          739 t
ORIGIN      
        1 tcaaaggcga tttgctcaac gaaggtgaat cggttgaatt tgaaaattta gtactcgaag
       61 tagttgataa agacggtcaa cgcatcgata aaatccttgt tacccgtatt caagaggaag
      121 aataattgat tttctatatc cacaaaaaag gactccttgt gagagtcctt cctatatttt
      181 ttgatgttca ttaaacaacc aaacaccaat tatcttcatc ggtaaactta cctgatttta
      241 cgccatctaa ataggttttt actttttgtg agaaatttgt ttcagcaact tctggcaaca
      301 caaaatcttt ccctctaaaa ttaatgcgtg taatgcttgc aatagttgct gctgttccag
      361 ctccaaaagc ttcttgcaaa cgcccttctt cggctgcttt tacaatttct tcaacagaaa
      421 ccttacgttc ttctactgga taaccccact tttgagcaac atcaacgatg gtacgcttag
      481 ttgttcctct caaaatagta tcttcatcct ctgctggagt aatcaacgta tcgttaataa
      541 caaaacaaat gttcatcgtt cccgattctt caaaatactt gtgttctttt gcatctgtcc
      601 acaacaactg gctatatcct tctttttgtg aaagcaatgc cggatataat gccgaagcgt
      661 agttaccagc agtttttgct cttccaacac cacctattgc agctctggtg tagtattctt
      721 ccactttcac agcaactggt ttagaataat aggctccaac tggacacaaa aacacgataa
      781 atttatagtt atccgatggt tttacaccaa ttgcctcatc tgttgcaaaa acaaacgggc
      841 gaatgtacaa agaatatcct tcattctttg gaacccattc tttgtcaact tcaaccaatt
      901 tgcgaattaa ttccacaaac acttcttctt ccattgcagg aatacacaat ctttcactgg
      961 attctgcaaa acgttttgcg ttcatttctg ggcgaaataa aacgacatct ccattctctc
     1021 tgcgaaatgc tttcattccc tcaaaaattg tttgtccata gtgcaaagcc gacattgcag
     1081 gatgcattgg tataggtcca aaaggaacga tttctggtgt ttgccatttt ccatctgtat
     1141 aatccattac caacatgtgg tcggtaaata cttttccgaa aggcaaatta ttccaatcaa
     1201 cttggtgaat ttttgtttgg ctagtaggtg tgatcttaac tgttattggt tcttttatca
     1261 tttcttgcta aaatttcctt caaatataat taaaaaatca ttgtgcaact ttccctattt
     1321 tgttaaataa acactaaaaa atcctatttt tgaaaactta agagccatgt ctaaaagttt
     1381 agaaacatta caaaaatatt ggggatacaa caattttcgt cctggacaag aagccatcat
     1441 tgacgatgtc atcaatgggc atgatgtgct tgcgttgatg cctacaggag gtgggaaatc
     1501 catttgcttt caagttcctg gactcattcg cgagggacta acactagtaa tttcgccttt
     1561 aattgcattg atgcaagacc aagtaacggc tttggataaa aaacacatcc ttgcaaaagc
     1621 tatcacaagt ggtatgagtt ttagagaatt ggatattaca ctcgataatg ctcgatttaa
     1681 cggctatcaa tttctttaca cttcgccaga aagaattcaa agtactcttt ttattgaacg
     1741 tttcaagctg atgaacatag ggctcattgt aattgacgag gctcattgta tttctgaatg
     1801 gggacatgat tttcgaccga gttttatgga aattaaaaag cttcgcgaaa ttcacccttc
     1861 tgttcctatc attgcactaa ctgcaacggc aacacctaaa actcagcaag aaattattga
     1921 acgtttggaa ctcaaaaatc caaaaattca tcaatctagt tttgaacgca aaaatttatc
     1981 ctacaaaagc tatttaacca acaataaact acaagaaatt attgacacct gcaagcaatt
     2041 tgaaggaaaa acaggaattg tttattgtca aaccagaaaa tcggtcaaat ttgttgcaca
     2101 aaagctctac agtcaacaac tttcggtagg catttaccat ggtggaatga atcgcgatga
     2161 acgcaaaaaa atgctcgaag attggctcag caataaaata aaaattattg tggctaccaa
     2221 cgcttttgga atgggaattg acaaacccga tgtgcgtttt gtgttgcact atgaattccc
     2281 caattcactc gaagcatatt ttcaggaagc aggacgtggt ggacgcgatg gaaacgaatc
     2341 ggttgcaatc aattat
//
LOCUS       DAOQVQ010000400         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_55875_length_2355_cov_3.012174, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000400 DAOQVQ010000000
VERSION     DAOQVQ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55875_length_2355_cov_3.012174"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /gene="rhuM"
                     /locus_tag="PKN22_10165"
     CDS             <1..566
                     /gene="rhuM"
                     /locus_tag="PKN22_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNS43112.1"
                     /translation="EIRLSERKFYQKITDIYATAIDYDVTAQATKRFYATVQNKLHWA
                     IHGQTAAEVIVNRADHQKENMGLTTWKDAPKGKIQKFDVSVAKNYLSENEMQQLQRLV
                     SAYLDIAEDMANRHIPMTMEDWENRLNKFIDATDREILQNAGSVTAEIAKVHAESEFE
                     KYRIIQDRLFESDFDREIKRIGEGSDE"
     gene            1064..2044
                     /locus_tag="PKN22_10170"
     CDS             1064..2044
                     /locus_tag="PKN22_10170"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNS43113.1"
                     /translation="MKTVQFREALREAMSEEMRKDRSVFLMGEEVAEYNGAYKVSQGM
                     LDEFGPERVIDTPIAELGFAAIGVGASMNGLRPIVEFMTWNFAILAADQIINSAAKML
                     QMSGGQYHCPIVFRGGNGQAGQLAATHSQSFESFYAHVPGLKVITPSTPYDAKGLLKS
                     AIRDNDPVVFLESEKMYGDKGEIPEGEYLIPIGVADTKRKGTDVTIVSFGKIIKVAHA
                     AAEELAKEGIEVEIIDLRTVRPIDYAAVVESVKKTNRCVVVEESWPLASISSEIAYHL
                     QRYAFDYLDAPVLRVTQSDTPFPFATSLMEEALPNVERIIKAVKTVLYRN"
     gene            2143..>2355
                     /locus_tag="PKN22_10175"
     CDS             2143..>2355
                     /locus_tag="PKN22_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNS43114.1"
                     /translation="MGYHFINDKYRNHWLLLASIFFYAWGAPLFIFVVLATIIVDFFL
                     VNQIYRATQHHKKKWLIASLLLNLGML"
BASE COUNT          771 a          390 c          477 g          717 t
ORIGIN      
        1 gagaaatccg cttgagcgaa cgaaagttct atcaaaaaat aacagacatt tatgcaacag
       61 cgattgatta tgatgtaact gcccaagcaa caaaacgttt ttatgctact gtacaaaaca
      121 agctgcattg ggcaatacac ggacaaacag ctgctgaagt tattgtaaac agagccgacc
      181 atcaaaaaga aaatatggga ttgactactt ggaaagatgc tccaaaagga aaaattcaaa
      241 aatttgatgt gagtgtggct aaaaattatc tttctgaaaa cgaaatgcaa caattacaac
      301 gtttagtctc agcttattta gatattgcag aagatatggc aaatcggcat attccgatga
      361 ccatggaaga ttgggaaaat cgcttaaata aatttataga tgccactgat agagaaatat
      421 tgcaaaatgc aggtagtgta actgcagaaa tagctaaagt ccatgcagaa agtgaatttg
      481 aaaaatacag aattattcaa gaccgattat ttgaaagtga ttttgataga gaaataaaac
      541 gaattggtga agggagtgat gaatgatgaa tgatgaatga tgaattgtga attacgaatt
      601 acgaattacg attttgaatt tacgaattgg gaattatgaa ttgtgagttg tttccctttg
      661 cgttcattgc gcctactttg tgctcattgc ggttacaatt aaattgtgaa ttgtgaattg
      721 tgaattgttt ctcttagcgt tcattgcggt ttaaaaaaac ttaaccgcgg agttacgcca
      781 agaacgcaca gagataaaca ttgcgccctt tgcgtaatcc tttgcgttca ttgcggttat
      841 aatataattc ctctttgcgt tcattgcgcc tactttgcgc tcattgcggt tactattaaa
      901 ttgtgaatta cgaatacgat atggacacga aaaaaataaa attaaaagta gagtagctga
      961 aacttgaatt cactatcaaa taatttagtc ctatttattg ctacaaatac caacattttt
     1021 ttaactttga cactttccaa atggaaaata gcattaattt gatatgaaga cagtacaatt
     1081 tagagaagcg ttgcgcgaag caatgtcgga agaaatgaga aaagacagaa gcgtcttttt
     1141 aatgggtgaa gaagttgctg aatacaacgg tgcatataaa gtttcacaag gaatgttgga
     1201 tgaatttggt ccagaacgcg tcatcgatac accaattgca gaacttggtt ttgcagctat
     1261 cggtgttggt gcgtcaatga acggtttgcg cccaattgtg gagtttatga catggaactt
     1321 tgccatcctc gctgccgatc aaattatcaa tagtgctgca aaaatgttgc aaatgtcggg
     1381 tggtcaatac cattgcccaa ttgtatttag aggtggtaac ggacaagctg gacaattagc
     1441 tgcaacacac tctcaaagct ttgagtcgtt ttatgcacac gtgccagggt tgaaagtaat
     1501 tacaccttca actccttatg atgcaaaagg tttgttgaaa tccgcaattc gcgataacga
     1561 cccagttgtg ttcttggaat cagaaaaaat gtatggagat aaaggtgaaa ttccagaagg
     1621 agaatatctt attccaattg gtgttgcaga tacaaaacgc aaaggaacag acgttaccat
     1681 tgtttcattt ggtaaaatta tcaaagtagc acatgcagct gctgaagaat tagcaaaaga
     1741 aggaatcgaa gttgagatca tcgacttgcg caccgttcgc ccaattgact atgcagcagt
     1801 tgttgaatcc gtgaaaaaaa caaaccgttg tgttgtggtg gaagaatcat ggccattggc
     1861 ttccatttct tctgaaattg cataccacct acaacgttat gcttttgact atttggatgc
     1921 accagtattg agagttacac aatcagatac accattccca tttgcaactt cgttaatgga
     1981 agaagcattg ccaaatgtgg aacgaatcat caaagctgta aaaacagtat tgtatagaaa
     2041 ctaacattca aggaggtgat tttcgcctcc tttttcaatt gataactact gatggtattt
     2101 agcagcattt atttcctgct ttattttctt ccaattgtat tgttgggata ccactttatt
     2161 aatgataaat acagaaatca ttggttgttg cttgccagca tttttttcta tgcttgggga
     2221 gctccactat ttatttttgt tgttttagct accatcattg ttgatttttt tctggtcaat
     2281 caaatttatc gtgcaacaca acaccataag aaaaaatggc tgattgcttc tttgttgctc
     2341 aatttaggaa tgttg
//
LOCUS       DAOQVQ010000401         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_56166_length_2347_cov_2.949389, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000401 DAOQVQ010000000
VERSION     DAOQVQ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56166_length_2347_cov_2.949389"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..785
                     /locus_tag="PKN22_10180"
     CDS             360..785
                     /locus_tag="PKN22_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS43115.1"
                     /translation="MKRATGIGGVFFKCKDPKKVREWYSKHLGLNTNAYGTVFEWYQG
                     ADSTKKGFTQWSPFNEKTTYFEPSTKDFMINFRVEKLEQLVAVLKSEGVTVLDTIETY
                     DYGKFVHILDIEGNKVELWEPNDVEFEKLGQQIGAQTTK"
     gene            799..1419
                     /locus_tag="PKN22_10185"
     CDS             799..1419
                     /locus_tag="PKN22_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43116.1"
                     /translation="MKTITKNSVAVGIICTLVFMAIAMLQYPGGTYLDVTTVGYDWTQ
                     NFISNLFKEKALNGAENSAQYWAFAGMFFYAFTCALFFIHTAKKIPNSTAANFVKYTG
                     ILTMPCILMVATPFHDLMLAVSNYLFWACIIAITVYLLKSKNYLLAFYALLCILIFYY
                     ATYLYIAQSWETLVIVQKVNNLSAIILIFSIEYFTTKESFLIAVKK"
     gene            1425..1967
                     /locus_tag="PKN22_10190"
     CDS             1425..1967
                     /locus_tag="PKN22_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNS43117.1"
                     /translation="MNTYIAILRGINVSGQKIIKMDALRQLFENSGFSNVRTYIQSGN
                     VLFSSEQTNTSELEQLIATQIEKEFGFQVPVLILTHLELQQIVENNPFLNDETKNPAF
                     LHITFLASTPVEDAIDQLTIKKAEQEAFLLTQNALYLYCPNGYGNTKLTNNFIESKLK
                     VQATTRNWKTTLELLKMAEK"
     gene            2038..>2347
                     /locus_tag="PKN22_10195"
     CDS             2038..>2347
                     /locus_tag="PKN22_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNS43118.1"
                     /translation="MVNFVLIAFSITAGMLFRTFNLIPKDAHKTINTWVLYLALPAVS
                     FKYIPKIQWSTEMLFPVLSVLLVWAGSWVFMEVYCRFKNYNQRSRSTLEIASGFSNTS
                     F"
BASE COUNT          804 a          412 c          392 g          739 t
ORIGIN      
        1 aaaatccaac attgtttgat gattatatta aacaaatata cacttttttt tataaattgt
       61 gtattataat catcaatttt taaccaacaa aatatcatgc tgttcggcaa taaaaaagtt
      121 attgaagcac cagaatttct tgcttgagga agcaaccatt caacatataa ataacaagca
      181 atttttagag gagatgtgtt tatattaatc ttaatttttt tattcccgaa aaaaaattca
      241 tttttacaca ctaaaacaat accatgaaaa agaaaatttt gttgggctta ttgctagtag
      301 ctacattttg ttcaggaatg gcatttgaca aattgatgtt tcaaaatgaa acgcccaaaa
      361 tgaagcgcgc aaccggaatt ggaggcgtat tttttaaatg caaagatcct aaaaaagtgc
      421 gcgagtggta cagcaagcat ctgggattaa acaccaatgc ctacggaact gtttttgaat
      481 ggtatcaagg ggctgacagt accaaaaaag gatttacaca atggagtccg ttcaacgaaa
      541 aaaccaccta ttttgagcct tctaccaaag attttatgat taactttcgc gttgaaaagc
      601 tagaacaatt agttgctgtt ttgaaatcag aaggcgttac cgttcttgac accatcgaaa
      661 cgtatgatta tggaaaattt gtgcatattt tagacatcga aggcaataaa gttgaactgt
      721 gggagcccaa cgatgtagag tttgaaaaat tagggcaaca aattggcgca caaacaacca
      781 aataatcctc gaattcttat gaaaactatt accaaaaatt cagttgcagt tggcatcatt
      841 tgtacgcttg tgtttatggc aatagccatg ctacaatatc ctggcggaac gtatttagat
      901 gtcacaaccg ttggatatga ttggacgcaa aattttatta gtaatttatt taaagaaaaa
      961 gcgttgaatg gggcagaaaa ctcagctcaa tattgggctt ttgcaggaat gtttttttac
     1021 gcatttactt gtgccttgtt ttttatccac acagctaaaa aaattcccaa ttccactgct
     1081 gctaattttg taaaatacac tggaatttta accatgccat gtattttgat ggttgcaaca
     1141 ccctttcacg atttaatgct ggcagtatcc aactatttat tttgggcgtg tatcattgca
     1201 attacggtct atttattaaa atcaaaaaac tatcttttag cattttacgc attgctttgc
     1261 attctcattt tttattatgc tacctatttg tatattgctc aaagttggga aacattggtg
     1321 attgttcaaa aagtaaacaa cctaagtgct atcattctca tttttagtat cgaatacttt
     1381 acaacaaaag aaagttttct aatagctgta aaaaaataaa tacgatgaac acctatattg
     1441 caattttacg aggcatcaat gttagtggac aaaaaatcat caaaatggat gcgttgcgcc
     1501 aactctttga aaattcaggt ttttccaatg ttcgaacgta tatccaaagt ggcaatgtat
     1561 tattttcaag cgagcaaacc aacacttctg aattagaaca actaattgct actcaaatcg
     1621 aaaaggaatt tggttttcaa gtaccagtac taattttgac acaccttgaa ttgcaacaaa
     1681 tcgtagaaaa caatcctttt ttgaacgatg aaactaaaaa tccagcgttt ttacacatta
     1741 cgtttctagc ttcaactcca gtagaggatg caatagacca actaacaatt aaaaaggcag
     1801 aacaagaagc gttcttacta acccaaaatg cgttgtatct ctattgtccc aacggttacg
     1861 gcaacaccaa gttgaccaat aattttattg agtcgaaatt aaaagtgcaa gccaccacca
     1921 gaaactggaa aacaacctta gaattattaa aaatggcgga gaaatagttt ttattacacc
     1981 aaattaccac taattttgca agcaatttta attgtaaatt aaattcagat acgtgctatg
     2041 gtgaattttg tattaatagc atttagtatc acggctggaa tgcttttcag aacattcaac
     2101 ctgattccaa aagatgctca taaaacaata aatacgtggg ttttatacct tgctctccct
     2161 gctgtatcgt ttaaatacat ccccaaaatt caatggtcaa ccgagatgtt gtttcctgtc
     2221 ctatctgtcc tattggtatg ggccggtagt tgggtgttta tggaagtata ttgccgtttc
     2281 aaaaattaca accaacgctc cagaagcacg ctggaaattg ccagtggatt tagcaatacc
     2341 tcattta
//
LOCUS       DAOQVQ010000402         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_56587_length_2335_cov_4.251754, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000402 DAOQVQ010000000
VERSION     DAOQVQ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56587_length_2335_cov_4.251754"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="lipB"
                     /locus_tag="PKN22_10200"
     CDS             complement(<1..615)
                     /gene="lipB"
                     /locus_tag="PKN22_10200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNS43119.1"
                     /translation="MKPKVIFQSLGLIDYQEAWDYQEKLFKDTIDKKIRIRNGETDLS
                     TQNYLLFCEHPHVYTLGKSGDASHLLLSEELLAQHQAKFYKINRGGDITYHGPGQLVG
                     YPIFDLDHFFPDIHKYMRFLEEAVIQTLKEYGIESGRVEGMTGVWLDTDKPTARKICA
                     MGVKSSRWVTMHGIGFNINSDLNYFSHIVPCGIQDKGVTSMKKEL"
     gene            complement(619..1599)
                     /locus_tag="PKN22_10205"
     CDS             complement(619..1599)
                     /locus_tag="PKN22_10205"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006423.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNS43120.1"
                     /translation="MDKLIAAFIANIEEALQIASNATFQTPKKTINNVVISGMGGSGI
                     GGKLVALWIQDEIKVPVQCFNDYVTPQFINENTLLIASSYSGNTEETLIFTKEAKDKG
                     AHIIGVCSGGELANFCKTNGFDCVVVPGGNPPRTALAFSIVQLTNIFYKLGLTSDKVL
                     NQLKSGGELIHARNAHIQEEAKKLATFLKDTVPAFYATTPYEAVLIRAKQQFNENSKL
                     LCWTHVIPEMNHNELVGWGGGDNRFAAVYFDTCDVYPRNAKRVEITLEVIEAKTKLMK
                     IKAEGSNFVERSIYLIHLVDWASYFLAEQKNVDAFDIKIIDRLKSELANF"
     gene            complement(1665..>2335)
                     /locus_tag="PKN22_10210"
     CDS             complement(1665..>2335)
                     /locus_tag="PKN22_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS43121.1"
                     /translation="LAQVTIEMLEKGKVYALIGSNGSGKSTFLKTLCGQIKPFSGDIA
                     IDNHSIQSLSKEQFSKKIAFVPSRFPEIEFTTVKDFVLLGRTPYLNNRGIVTKQDLEY
                     VDGILSRVNIQHLTTKYTNELSDGERQMCAVARALAQQTPIILLDEPSSFLDYKNKRR
                     LIGLLLQIAKEMNLLILFSSHDIETIVQFDIQLLGLSCAPSKKIVTIDNKMDFEQLIE
                     QFYE"
BASE COUNT          703 a          466 c          382 g          784 t
ORIGIN      
        1 taattctttt ttcattgaag taacgccttt atcttgaatg ccacacggta caatgtgcga
       61 aaaataattc aaatccgagt taatgttaaa ccctattccg tgcatggtaa cccaacgtga
      121 gcttttaact cccattgcgc aaatttttcg agccgttggt ttatcggtat ccaaccaaac
      181 acccgtcatg ccttctaccc ttcccgactc gatgccgtat tctttcaatg tttgaataac
      241 agcttcttcc aaaaaacgca tgtatttgtg aatatctgga aaaaaatggt ctaaatcaaa
      301 aataggatag cctaccaatt gtccgggacc gtgataggta atatctcccc cgcgattgat
      361 tttataaaat ttagcttggt gctgtgccaa caattcttcg cttaaaagca aatgcgaagc
      421 atcaccactt ttcccaagtg tatacacatg tgggtgctcg caaaacaaca aataattttg
      481 agtggataaa tcggtttcgc cattgcgaat gcggattttc ttatcaatcg tatctttaaa
      541 cagtttttct tgataatccc acgcttcttg ataatctatc aatccaagtg attgaaagat
      601 aacttttggt ttcatttctt aaaagtttgc taattctgac tttaaacggt caatgatttt
      661 aatatcaaaa gcatcaacat ttttttgttc tgctaaaaag taagaagccc aatctaccaa
      721 atgaatcaaa taaatggaac gttccacaaa attagaacct tctgctttga ttttcatcaa
      781 ttttgttttg gcttcaatca cttccaaggt gatttctact cgttttgcat ttcttggata
      841 cacatcgcac gtatcaaaat aaacggcagc aaaacggtta tcgccaccac cccagccaac
      901 tagttcattg tggttcattt ctggaatgac atgtgtccaa cacaacaatt tagaattttc
      961 attgaactgt tgcttagcgc gaattaatac agcctcgtat ggtgttgttg cataaaatgc
     1021 tggcactgta tcttttaaaa atgttgccaa ctttttagct tcctcctgga tgtgtgcatt
     1081 tcttgcgtga atcaactcac cccccgactt taattgattt aaaactttat ctgaggttaa
     1141 tcctaactta taaaatatgt tggtcaactg aacaatggag aatgccaatg cagttcttgg
     1201 agggtttcca cctggcacta caacgcaatc aaaaccgttt gttttgcaaa aattggctaa
     1261 ttcaccaccc gaacatacac cgattatgtg agctcctttg tccttcgctt ctttggtaaa
     1321 aattaatgtt tcttctgtat ttcctgaata ggatgaagca attaacaatg tgttttcatt
     1381 aataaattgc ggtgttacat agtcattaaa acattgaact ggtactttga tttcatcttg
     1441 aatccaaaga gcaactaatt ttcctccaat accagagcca cccatgccac taatcacaac
     1501 attgttgatt gtttttttag gcgtttgaaa ggttgcatta ctagcaattt gtaaagcttc
     1561 ttcaatattt gcgatgaatg cggcaatcag tttgtccatg cgagtattaa ttttggtgta
     1621 aaaatagtga ttctaatgaa ttattgatgt tcaaagtttt tatttcattc ataaaattgt
     1681 tcaattaact gctcaaagtc cattttattg tcaattgtta caattttttt tgatggtgca
     1741 cagcttaatc caagtagttg aatatcaaat tgaacaatag tttcaatatc gtgtgacgag
     1801 aacaaaatga gtaagttcat ttctttggca atttgaagca aaagcccaat caatcgacgt
     1861 ttatttttgt aatccaaaaa gctactaggt tcgtctaaca aaataatagg tgtttgttgc
     1921 gccaaagctc tggccacagc acacatctga cgttcaccat cacttagctc attggtgtat
     1981 tttgtggtta aatgttggat gtttacccga ctcaaaatgc cgtccacata ttctaagtct
     2041 tgcttggtca caataccccg attattcaaa taaggtgttc gccccaacaa cacaaaatct
     2101 ttaaccgttg taaattcaat ttctggaaaa cgtgaaggaa caaaagcaat ttttttagaa
     2161 aactgttctt tcgataaact ttgaatggag tgattatcaa tagcaatatc gccagaaaat
     2221 ggtttgatct gaccacaaag cgttttcaaa aacgttgatt ttccgctacc attgctaccg
     2281 atgagcgcat atacctttcc tttttcaagc atttctatcg ttacttgagc taagg
//
LOCUS       DAOQVQ010000403         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_56980_length_2325_cov_4.561674, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000403 DAOQVQ010000000
VERSION     DAOQVQ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56980_length_2325_cov_4.561674"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /gene="lon"
                     /locus_tag="PKN22_10215"
     CDS             <1..1371
                     /gene="lon"
                     /locus_tag="PKN22_10215"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNS43122.1"
                     /translation="DHYGLKKVKERILEHLAVLKLKGNMKAPILCFYGPPGVGKTSLG
                     KSIAEAMGRKYVRMSLGGVHDESEIRGHRKTYIGAMPGRVVQNLKKAEAANPVFILDE
                     IDKLGRSNHGDPSSALLEVLDPEQNSAFYDNYVETEMDLSKVMFIATANNLADIQAPL
                     RDRMELIEVNGYTIEEKIEIAKQHLLPKQIEAHGLTKKDISLDKKVLEKLVEEYTNES
                     GVRGLEKMVAKLVRNRARQIAMEESFEVKITEVDLKTILGPGRQKTKYDNNNIAGVVT
                     GLAWTSVGGDILFIESSITKGKEKLTLTGNLGDVMKESAVIALEFLKSHSHWLNLNQE
                     VFENSNVHIHVPEGATPKDGPSAGITMLTSLASSFTQRKVKKNLAMTGEITLRGEVLP
                     VGGIKEKILAAKRAKIKEIILCEKNRQDIEEIEPEYLTGLTFHYVTRMKEVLDIALTK
                     EKAKGE"
     gene            complement(1379..2035)
                     /locus_tag="PKN22_10220"
     CDS             complement(1379..2035)
                     /locus_tag="PKN22_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNS43123.1"
                     /translation="MVSQTEENYLKALFHLTTETEGKQDAGTNELAVFLEVKPATVSD
                     MLKKLKEKELVAYEKYGKIALTEKGRDYAVMVIRKHRLWETFLYEKLNFSWDEVHEVA
                     EQLEHIQSKKLVEELERFLGFPEIDPHGDPIPNAKGQIKMVQRQTLAQIATGKTCKLV
                     AIKDSSASFLQYALQLGLGLSTQIKIKNRHDFDGSLEIEINGKPSTVSQKFAENVFVV
                     "
     gene            2115..>2325
                     /locus_tag="PKN22_10225"
     CDS             2115..>2325
                     /locus_tag="PKN22_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43124.1"
                     /translation="MSNLRLLIVFILYQSVAFSQTIIKGIVKDAQTHLPIEFCSIIQQ
                     NTTNGALTNDKGLFTITVDVFPTHLQ"
BASE COUNT          774 a          417 c          456 g          678 t
ORIGIN      
        1 gaccactacg gactcaaaaa agtgaaagaa cgtattttgg aacatttggc agttttaaag
       61 ttaaaaggta acatgaaagc accaattttg tgtttttatg gacctccagg tgtaggaaaa
      121 acttcgttag gtaaatcaat tgccgaggca atgggcagaa aatacgtgcg catgtccttg
      181 gggggtgtac acgatgaatc tgaaattcgt ggccatagaa aaacatacat tggagcaatg
      241 cccggcagag tggtgcaaaa cttgaaaaaa gccgaagcag ccaatccggt gtttattctc
      301 gatgaaattg acaaattggg aagaagcaat cacggtgatc catcatcggc attattggaa
      361 gtacttgatc ctgagcaaaa cagtgctttt tacgacaatt atgttgaaac cgaaatggat
      421 ttgtcaaaag taatgttcat tgcaacagca aataatttgg cagatattca agctcctttg
      481 cgcgatcgca tggagttgat tgaagtaaac ggctatacaa ttgaagaaaa aattgaaatt
      541 gcaaaacaac atttgttgcc caaacaaatt gaagcacacg gcttgaccaa aaaagacatt
      601 tcgttagaca aaaaagtgct tgagaaatta gttgaagaat ataccaacga gtcgggagtg
      661 cgtggtttgg aaaaaatggt agccaaattg gtgcgcaacc gagcacgtca aattgctatg
      721 gaagaaagct ttgaggttaa aattaccgaa gtagatttga aaactattct tggtcctgga
      781 cgccaaaaaa ctaaatatga taacaacaac attgctggtg tggttacagg tttggcgtgg
      841 acaagtgttg gaggcgatat tttattcata gaatcttcta tcaccaaagg aaaagaaaaa
      901 ttaacgttga caggaaactt gggagatgtg atgaaagaat cggcagttat tgctcttgaa
      961 tttttgaaat cgcacagcca ttggttgaat ttaaaccaag aagtgtttga aaactcaaac
     1021 gtacacatac acgtgcctga gggagcaaca ccaaaagatg gtccaagtgc tggtattacg
     1081 atgttaacgt ctttggcatc atcgttcaca caacgaaaag tgaagaagaa cttggcaatg
     1141 acaggtgaga taacgttacg tggcgaagta ttgccagttg gcggcattaa agaaaaaatc
     1201 ttagcggcaa aacgtgccaa aatcaaagaa attattttgt gcgaaaaaaa ccgacaagat
     1261 attgaagaaa ttgaacctga atatttaacc ggtttgacat ttcactacgt gacgcgcatg
     1321 aaagaagtat tagacatcgc cctcacaaaa gaaaaagcaa aaggagagta gtttttgact
     1381 acaccacaaa tacattttcg gcaaattttt gacttacagt tgatggtttg ccgtttattt
     1441 caatttctaa agaaccgtca aaatcatggc ggttttttat ttttatttgg gtgctcaagc
     1501 ccaaacctag ttgcaacgca tattgcaaaa acgaagcact actgtcttta atagcaacta
     1561 atttacatgt tttaccagtt gcaatttggg caagtgtttg gcgttgaacc attttgattt
     1621 gacccttggc attgggtatc gggtcgccat gaggatctat ctctggaaaa cctaaaaaac
     1681 gctccaattc ttccaccaat tttttggatt gtatgtgctc caattgctcg gcaacctcgt
     1741 gcacttcgtc ccaactaaaa tttaattttt catataaaaa agtttcccaa agtcggtgct
     1801 tgcgaatgac catcaccgca taatcccttc ctttttcggt aagagctatt tttccatatt
     1861 tttcataggc aaccaactct ttttctttga gttttttcaa catatcgctg accgtagctg
     1921 gttttacctc caaaaaaacc gccaattcat tggtgccagc atcttgtttt ccctctgttt
     1981 ctgtggttaa atgaaacaat gcctttaaat aattttcttc tgtttgtgat accattctac
     2041 aaatatatga aagatttcta taaatttatt tgttagataa gtctaactat attagttttg
     2101 caaataaaat acacatgtca aacttacgat tattaatagt attcattctg tatcaatcgg
     2161 ttgcatttag tcaaacaata attaaaggca ttgtaaaaga tgcacaaaca catttaccga
     2221 ttgagttttg ttccattatt caacaaaata caaccaacgg agcactcacc aatgataaag
     2281 gattgtttac tattaccgtt gatgtttttc caacgcatct tcaaa
//
LOCUS       DAOQVQ010000404         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_56986_length_2325_cov_3.695154, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000404 DAOQVQ010000000
VERSION     DAOQVQ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56986_length_2325_cov_3.695154"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKN22_10230"
     CDS             complement(<1..732)
                     /locus_tag="PKN22_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNS43125.1"
                     /translation="MEENQAKERISYLVGYLNQCNYEYYVESNPTISDFEFDQLLKEL
                     ETLEKQFPQFAVDYSPTKRVGGDITKKFEVVKHTYPMLSLSNTYSEEEIVDWANRIKK
                     LISEPLEYVCELKYDGVAIGIKYENGILTQAVTRGDGTQGENITANVKTIRTVPLQLK
                     GNFPATFEIRGEIFLPFKKFDAINKEREDLGEPLFANPRNSASGTLKLQDSKVVAARG
                     LDTYLYGVYGVEGLATGHYEMVQQAG"
     gene            865..1086
                     /gene="ccoS"
                     /locus_tag="PKN22_10235"
     CDS             865..1086
                     /gene="ccoS"
                     /locus_tag="PKN22_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNS43126.1"
                     /translation="MGIIYIMLIVSLIIAIIFLIGFLWATKTGQFDDDYTPSVRILFE
                     DESVEQKTETEKEKKEKQQNQTEHGNSKV"
     gene            1067..>2325
                     /gene="ccoN"
                     /locus_tag="PKN22_10240"
     CDS             1067..>2325
                     /gene="ccoN"
                     /locus_tag="PKN22_10240"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNS43127.1"
                     /translation="MEIQKFSYDNKIVKYFAYATIIWGIIGMLVGLTIALQLAFPEFF
                     NDYLPLSWLSFGRIRPLHTNAVIFAFVGNGIFTGVYYSLQRLLKTRMWSDKLSYINFW
                     GWQFIIVCAVITLPLGISTSKEYAELEWWIDIMIAGIWVVWGWNMFATILNRRVKHLY
                     VAIWFYIGTFVAVAMLHIFNSFEIPVSFLKSYSWYAGVQDALVQWWYGHNAVAFFLTT
                     PYLGLMYYFVPKAANRPVYSYRLSIIHFWALIFMYIWAGPHHLLYSSLPDWAQSLGVI
                     FSVMLIAPSWGGMLNGLLTLRGAWDRVRDDVTLKFMVVALTGYGMSTFEGPLLSLKNV
                     NVISHFTDWTVAHVHVGGLAWNGMLTFGMLYWLFPKMFRTKLYSKKMANQHFWIATLG
                     IILYVIPIYIAGFVQGLMWQEFNPDGT"
BASE COUNT          685 a          373 c          456 g          811 t
ORIGIN      
        1 tcctgcttgt tgcaccattt cgtagtgacc agtagccaac ccttcaacgc cataaacacc
       61 atataaatac gtgtctaaac cgcgcgctgc cacaacctta gaatcttgta attttaacgt
      121 tccagaggcc gaattgcgag gattggcaaa caagggttct cctaaatctt cacgttcttt
      181 gttaatggca tcaaactttt tgaagggcaa aaatatctcc ccacgaattt caaatgttgc
      241 agggaagtta cctttaagct gcagcggaac ggtgcgaatg gttttaacat tggcggtaat
      301 gttttcgcct tgcgttccat cgccacgagt aacagcttgc gttaaaattc cattttcgta
      361 tttaatacca attgcaacac cgtcgtattt caattcacac acatattcaa gcggttcgga
      421 aattaatttt ttaatacgat ttgcccaatc aacaatttct tcttccgaat aggtgtttga
      481 aagcgaaagc atggggtagg tatgcttaac aacttcaaat tttttggtga tatcgccgcc
      541 tacacgtttg gttggagaat aatcaacagc aaattgagga aattgttttt ctaatgtttc
      601 taattccttt aacaattggt caaattcaaa atcactaatt gttggattgc tttcaacata
      661 gtattcataa ttgcattgat taaggtaacc aactaagtat gagattcttt cttttgcttg
      721 attttcttcc ataggctaaa attaattttt ttaacggaaa taaaagtttt acctttaaaa
      781 ttatttttta agatttttta agaaaaaatg gaaaatatga caattatcat tttttatgaa
      841 tgacaattaa atgacatttg caccatggga ataatataca taatgttaat cgtgagttta
      901 attattgcaa ttatcttttt aataggcttt ttgtgggcta ctaaaacggg gcaatttgac
      961 gatgattata caccaagtgt aagaattctc tttgaagacg aaagtgttga acaaaaaaca
     1021 gaaacagaaa aagaaaaaaa agaaaaacaa caaaatcaaa cagaacatgg aaattcaaaa
     1081 gtttagttac gacaataaga ttgtcaagta ctttgcctac gccaccatca tttgggggat
     1141 aataggtatg ttggttggtt taactattgc attgcagttg gcctttccag aattttttaa
     1201 cgattactta ccattgagtt ggttatcgtt tggtagaatt cgtcctttac acaccaatgc
     1261 ggtgattttt gcatttgtag ggaacggtat ttttacaggt gtttattatt ccttacaacg
     1321 tttgttaaaa acacgtatgt ggagtgataa attgagttac atcaactttt ggggatggca
     1381 atttatcatt gtttgtgcag ttataacact tcctttaggg atttcaacat caaaagaata
     1441 tgcagagttg gagtggtgga ttgacatcat gattgctggt atttgggtag tttggggttg
     1501 gaacatgttt gcaacaatat taaaccgaag agtaaaacat ttgtatgttg caatttggtt
     1561 ttatatagga acatttgttg ctgttgcaat gttgcacatt ttcaattcat ttgaaattcc
     1621 tgttagtttc ttgaaaagtt attcatggta tgcaggtgtt caagatgcgt tggtgcaatg
     1681 gtggtatgga cacaatgcgg ttgcattttt cttaacaact ccttatttag ggttgatgta
     1741 ttattttgta cctaaagcag caaacagacc ggtttattcc taccgcttat caatcatcca
     1801 cttctgggca ttgattttca tgtatatctg ggctggtcct caccacttat tatattcatc
     1861 attgccagat tgggcacaat cattaggggt aattttctct gtaatgttga ttgctccatc
     1921 ttggggtggt atgttgaacg gattgttaac acttcgtggt gcttgggata gagttcgcga
     1981 tgatgtgact ttgaaattca tggtggttgc cttaactggt tatggtatgt ctacttttga
     2041 aggtccactt ttatcgttga aaaacgtaaa tgttatttct cactttactg actggacagt
     2101 agcgcacgta cacgtaggag gtttagcttg gaacggtatg ttgacatttg gtatgttgta
     2161 ctggttgttc cctaaaatgt tccgtacaaa attgtactct aaaaaaatgg caaatcaaca
     2221 cttctggatt gcaacattgg gtattatttt atatgtaatt ccaatttata ttgcaggatt
     2281 tgtacaaggg ttgatgtggc aagaatttaa tccagatgga acttt
//
LOCUS       DAOQVQ010000405         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_56987_length_2325_cov_3.671366, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000405 DAOQVQ010000000
VERSION     DAOQVQ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56987_length_2325_cov_3.671366"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1190
                     /gene="tsaD"
                     /locus_tag="PKN22_10245"
     CDS             168..1190
                     /gene="tsaD"
                     /locus_tag="PKN22_10245"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNS43128.1"
                     /translation="MKEKETIILGIESSCDDTSASVLKNTAILSNIIASQKIHEEYGG
                     VVPELASRAHQQNIIPVVDAAIKKAGINKEDIDAIAFTNGPGLMGSLLVGTSFAKAFS
                     LALNIPMINVHHMKGHVLAHFIDDENKEKPQFPFLCLTVSGGHTQIVLVKDYLNMEII
                     GSTIDDAAGEAFDKSAKLLGFPYPGGPLIDKHAQNGNPHRFEFNKPVMNNYDFSFSGL
                     KTSILYFIQKQEKINPQFVQENLDDLCASIQHTIIEILMKKLQLAAKELNIKEIAIAG
                     GVSANKGLRNTLEQKAKQLGWKVYIPKFEYCTDNAAMIAITGKYMFDKGLFADQTITA
                     KPRVEL"
     gene            1298..1726
                     /locus_tag="PKN22_10250"
     CDS             1298..1726
                     /locus_tag="PKN22_10250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNS43129.1"
                     /translation="MKHLTSTLEIPVRFSETDAMGVVWHGNYLKFFEDAREHFGKVYG
                     LDNLQIHDKGFFIPIVRSEINHKASLHYGETAVVHSKLIYKESAKVVFEFKVIIKETG
                     QIAATGSTTQVFLDAKTRLLELAKPDFIEEWENKQNWINE"
     gene            1719..>2325
                     /locus_tag="PKN22_10255"
     CDS             1719..>2325
                     /locus_tag="PKN22_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNS43130.1"
                     /translation="MNNTVYLSYGTFVSPLGVGIEANFNALKNGESGVQLQPKSGFNQ
                     EDWYFSKINTLPENRYDTLLKLALADLKKQYSTAVLQDAQTLFIVSSTKGNIDALPND
                     VFASSREIIQKETNNPNNVIIISNACISGVLAINAAADYIQFSGYKTVIVIGIDVVSD
                     FVLYGFQSLYALSDAPVQPFDKNRKGISLGEACGIVVLSKDQ"
BASE COUNT          769 a          383 c          431 g          742 t
ORIGIN      
        1 gataaaaatt ccgttgcata agctgccaac tttgtttgta cgggacttgt gcgaatggca
       61 aaagcaaaaa caatgagcaa caacaccaca atctctacga ttgaatgtat tgttcgtcct
      121 attattttca gtacttttgt catataattt gaacaaattt aatcatcttg aaagaaaaag
      181 aaacaattat cttaggaatt gagtcatctt gtgatgatac ttctgcttcg gtgctcaaaa
      241 atactgccat tttatcaaat atcattgcta gtcaaaaaat tcatgaagaa tatggtggtg
      301 tggtgcctga attggcaagt agagcacatc aacaaaacat cattcctgtg gtggatgcag
      361 ctataaaaaa agcaggaatt aataaggaag atattgatgc cattgctttt acaaacggac
      421 cgggtttaat gggttctttg ttggttggaa cttcttttgc caaagctttt tcgttggctc
      481 ttaacattcc gatgattaac gttcatcaca tgaaagggca cgttttggcg cattttattg
      541 acgatgaaaa caaggaaaag cctcaatttc cttttttgtg tttaacggtt tcgggcgggc
      601 atacacaaat tgttttggtt aaagactatt taaatatgga aattattggt tctaccattg
      661 atgatgctgc tggtgaagct tttgataaat cagcaaaatt attgggtttt ccctaccctg
      721 gcggaccatt gattgataaa catgcacaaa atggcaatcc ccatcgtttt gagttcaaca
      781 aaccggtaat gaacaactat gattttagtt ttagcggatt gaaaacctct attttatatt
      841 ttattcaaaa acaagaaaaa ataaatccac agtttgttca agaaaattta gatgatttgt
      901 gtgcttctat tcaacacacc attattgaaa ttttaatgaa aaaattgcaa cttgctgcca
      961 aggaattgaa catcaaagaa attgcaattg caggtggagt gagtgccaac aaaggcttgc
     1021 gcaatacttt agaacaaaaa gcaaaacaat tgggttggaa ggtttacatt cctaaatttg
     1081 aatattgcac cgataatgct gccatgattg ccattactgg taaatacatg tttgacaaag
     1141 ggttatttgc cgaccaaaca ataacagcaa aaccacgtgt tgaactttaa ttgctacgca
     1201 aaaaaacgta tttatttacc aaatgaggat aaaaccatat tcattattgg catattgtag
     1261 ataatttcta aatttgtcgc ttaattctaa ctgcaacttg aaacatttaa catccacatt
     1321 agaaattcct gttcgtttta gtgaaacgga tgctatgggc gttgtatggc acggcaatta
     1381 tttgaagttt tttgaagatg cccgcgagca ctttggaaaa gtgtatggtt tagacaatct
     1441 tcaaattcac gataaaggtt tttttattcc cattgttcgt tcagaaatca accataaagc
     1501 gtctttgcat tacggagaaa cggctgtagt tcattcaaaa ctgatttata aagaatcggc
     1561 aaaagtggtg tttgaattta aagttatcat caaagaaact ggtcaaattg ctgcaacggg
     1621 tagcactacc caagtttttt tggatgctaa aacacgcttg ttggaattgg caaaacccga
     1681 ttttattgaa gaatgggaaa ataaacaaaa ttggataaat gaataataca gtttatttat
     1741 catacggcac ttttgtttct ccattgggcg ttgggattga agctaatttt aacgcactaa
     1801 aaaatggtga aagcggtgta caattgcaac caaaatctgg ttttaatcaa gaagattggt
     1861 atttttcaaa aatcaacact cttccagaaa atcggtatga tactctgtta aaattggcac
     1921 ttgctgactt aaaaaaacag tactcaacag ctgttttaca agatgcacaa accttgttta
     1981 ttgttagttc tacaaaagga aatatagatg ctttgcccaa cgatgttttt gcatcttcaa
     2041 gagaaattat tcaaaaagaa accaacaatc caaacaacgt aatcatcatt tcaaacgctt
     2101 gtatttcagg tgttttagca attaatgcag cagccgacta cattcaattt tcgggctata
     2161 aaacagtgat tgttatcgga atagatgttg tttccgactt tgttttgtat ggttttcaat
     2221 cgttgtatgc acttagcgat gcccctgttc agccgtttga taaaaaccga aaaggaattt
     2281 ctttaggaga ggcttgtgga attgttgtgc tttccaaaga tcaac
//
LOCUS       DAOQVQ010000406         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_57069_length_2323_cov_3.427690, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000406 DAOQVQ010000000
VERSION     DAOQVQ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57069_length_2323_cov_3.427690"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PKN22_10260"
     CDS             complement(<1..1067)
                     /locus_tag="PKN22_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43131.1"
                     /translation="MKFVDKIAQFIQQNKLEHKNLVIVVPSQRAKKYLSAALFNCYKK
                     PIIAPKMTTMDALIRSKSNRVILDKTRLLVKLFSIQQTIAKTDIDRSFDDFLEWGPLL
                     LSDFDELDRYLVNPEKAFKDLHNIKELEYWKIDEEADFVVSETRKRFLEFWDRLPFYY
                     QELNKQLNSEGATYMGSAYQYLVNNIDLLTRDNEIYLFAGFNALSAAETSIIKQLMKY
                     GKGHVLVDADAFYVKNNNHEAGAFIRKQLNDFDVKQLPFIEDHLAHKSMKIEVVECAQ
                     QTGQVKVAATRLLEMTPSDMPHTLVVLADESLIGPLLKNIPKNVGKTNITLGMPMKGT
                     AVRNWVDVLFAIQTTNERSAT"
     gene            complement(1067..2197)
                     /locus_tag="PKN22_10265"
     CDS             complement(1067..2197)
                     /locus_tag="PKN22_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNS43132.1"
                     /translation="MNRLQQQQLIEKIQTSKIVLVSGPRLSGKEEMIAQALTDLQLEH
                     LAMNLGNKKERKIIEEADEATLIQTLSAYKYIVIHEAQYLSNLQTIIELVLSDSISAS
                     ILLNCSFEPKMDDLLKEVLDTQGLHLKIYPPALYELTTHFGMAEESKLLEQRLINGNY
                     PAVTLSPDAAQDILSHLLDTVLITDLGVTDRINKKQQLIKMLQTLAFFIGEPVSYNEV
                     ATHAGIDNETVERYIELLEKAYVLIRIPSYSTEKRYELKKTHLIYFVDTGLRNMLINN
                     FNPPSVRMDMDALWKNWLVSERVKWNAINNKQVNYWFWRTHTKQTVDFIEEDENKNKV
                     GYKIAWDKKKKVKFPPMFGEYYPSIPTKVLNRTTYITFLASK"
BASE COUNT          675 a          441 c          410 g          797 t
ORIGIN      
        1 tcagttgccg aacgttcatt tgttgtttga atggcaaata aaacatctac ccaatttctt
       61 actgccgttc ctttcatcgg catacccagt gtaatgtttg ttttaccaac atttttagga
      121 atatttttga gcaacggacc aatgagcgat tcatcggcta aaaccaccaa ggtatgtggc
      181 atatcagacg gtgtcatttc caacaaacgt gttgcagcca cttttacttg tccggtttgt
      241 tgggcacatt ctaccacttc aattttcatg gatttgtgcg ccaaatgatc ttcaataaac
      301 ggcagttgct tgacgtcaaa atcgttgagt tgcttgcgga taaatgcacc tgcttcgtgg
      361 ttgttgtttt tcacataaaa agcatctgca tcaaccaata cgtggccttt tccatatttc
      421 attaattgct taatgatgct tgtttcggca gctgataggg cattgaatcc tgcaaacaaa
      481 taaatttcgt tatcacgagt caacaaatca atgttgttta ccaaatactg ataagcactt
      541 cccatatagg ttgcaccttc actgttgagt tgcttgttga gttcttggta ataaaacgga
      601 agacgatccc aaaattccaa aaagcgtttt ctggtttcgg agacaacaaa atcggcttct
      661 tcgtcaattt tccaatattc caactctttg atgttgtgta agtctttaaa tgctttttcg
      721 gggttgacta aatatcggtc taactcgtca aaatctgaca aaagcaaagg tccccactcc
      781 aaaaaatcat caaacgaacg atcgatatct gttttagcaa tcgtttgttg aatagaaaaa
      841 agtttaacca acaaacgcgt tttatctaaa attaccctat tggatttgga gcgaatcaac
      901 gcatccatgg tcgtcatttt aggagcaata attggttttt tgtagcaatt aaacaacgca
      961 gccgacaaat attttttcgc acgttgtgag ggtacaacaa tcactaaatt tttgtgttct
     1021 aatttatttt gttgaataaa ttgcgctatt ttatctacaa acttcattat ttagaggcca
     1081 aaaatgtaat gtaagttgtt ctgttcaaca cttttgtagg aatagaagga taatattcac
     1141 caaacatagg tggaaatttt actttctttt tcttatccca tgcaattttg tatcctactt
     1201 tgtttttatt ttcatcttct tcaataaaat caactgtttg cttggtgtgg gtgcgccaaa
     1261 accagtagtt aacttgttta ttgttgatgg cattccattt aacacgttcg gataccaacc
     1321 aatttttcca caaagcatcc atatccattc gcaccgatgg tggattgaag ttattgataa
     1381 gcatgtttct aagtcccgta tccacaaaat aaatcaaatg tgtttttttc agttcatagc
     1441 gtttttcggt agaataagaa ggtatgcgaa tcaatacgta ggctttttct aacaattcaa
     1501 tgtaacgttc aaccgtttcg ttatcaattc cggcatgagt tgccacttcg ttatacgata
     1561 ccggctcgcc aataaaaaat gccaaggttt gcaacatttt tatgagttgt tgttttttat
     1621 tgatgcgatc ggtcaccccc aaatcggtaa tcaaaacggt atccaataaa tgcgataaaa
     1681 tatcttgagc tgcatctgga gaaagagtaa ctgctggata atttccgtta attaaacgtt
     1741 gctccaacaa tttgctttcc tcagccattc caaaatgcgt ggtcaattca tacaacgccg
     1801 gcggataaat tttaagatgc aatccttgcg tatccaaaac ttctttgagc aaatcatcca
     1861 ttttaggttc aaacgaacag ttgagtaaaa tagaggccga aatgctatcg gataatacca
     1921 actcaatgat tgtttgtagg ttacttaaat attgtgcttc gtgaataaca atgtatttat
     1981 aggccgacaa ggtctgaatc aacgtggctt catcggcttc ttcaataatt ttacgttctt
     2041 ttttattgcc gagattcatt gccaaatgct ccaattgcaa atcggttaat gcctgagcaa
     2101 tcatctcttc tttacccgac aaacgtgggc ctgataccaa tacgattttg cttgtttgta
     2161 ttttttctat aagctgttgt tgttgtaatc tgttcattgg ttttaaccta ttttttgaga
     2221 ttacgaatat agagatttga aattaaatga atagggtgtg ttgataaatc ttcaactagt
     2281 tccttgtaaa atcgggcttt accacagtgg attgttagta aat
//
LOCUS       DAOQVQ010000407         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_57459_length_2313_cov_3.521701, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000407 DAOQVQ010000000
VERSION     DAOQVQ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57459_length_2313_cov_3.521701"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PKN22_10270"
     CDS             complement(<1..894)
                     /locus_tag="PKN22_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNS43133.1"
                     /translation="MFALIDCNNFYASCERVFRPDLNGKPIVVLSNNDGCVIARSNEA
                     KALGIPMGAAAFEYENMFKHHHVHVFSTNFALYGDMSNRVMTILSDYSPEMEIYSIDE
                     CFLKLHGFDTYFNLNDYGLNMRKKVGKWTGIPISVGIAPTKALAKAANRIAKKFPERT
                     NGVYVIDSEEKRIKALKWLKIEDVWGIGRQHAKRLIAQQVTSAYDFTQLPDSWVRKTM
                     AIVGLRLKHDLQGIPTFNLEEVQPKKSIATTRSFETNYTEFNQLKERITTFAVACAEK
                     LRQQQSCCNALLVFIHTNGHRK"
     gene            complement(896..1345)
                     /gene="umuD"
                     /locus_tag="PKN22_10275"
     CDS             complement(896..1345)
                     /gene="umuD"
                     /locus_tag="PKN22_10275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNS43134.1"
                     /translation="MAFLESHPHSVVELFNANTATRLHLPYVSDGISAGFPSPALDFI
                     DLTIDLNKHLIKHPTATFYGRVKGVSLKNAGIGDGDLLIIDRSLEPANGKIAVCYIDG
                     EFTAKRIRLAKNELWLVPENENYQPIRITEDTNFLIWGIVTHVIKDL"
     gene            1608..>2313
                     /locus_tag="PKN22_10280"
     CDS             1608..>2313
                     /locus_tag="PKN22_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNS43135.1"
                     /translation="MEKTSQQIATVEQAVELGLRQDEFEMIKEILGRTPNFTETAIYS
                     VMWSEHCSYKNSILWLKKLPKDGPHMLVKAGEENAGLVDIGGGLGCAFKIESHNHPSA
                     LEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGNIDRDRTKWLVRGVVKGIGDYGN
                     AFGIPTVGGEVFFDDSYNTNPLVNAFSAGILDSKKIISAKAKGPGNPVFIVGSATGKD
                     GIAGAAFASKDITEESA"
BASE COUNT          698 a          423 c          513 g          679 t
ORIGIN      
        1 tttgcggtgt ccgttggtgt ggataaatac taagagtgcg ttgcaacacg attgttgttg
       61 tcgcaatttc tcggcacagg caaccgcaaa agttgtaatt cgttctttta attggttgaa
      121 ttcggtataa ttggtttcaa agctgcgagt ggtggcaatg ctttttttag gttgcacttc
      181 ttccaggttg aacgtgggaa tgccttgcaa atcgtgtttg aggcgcaaac caacaatagc
      241 cattgttttt ctgacccaac tatcggggag ttgcgtaaaa tcataggcgc ttgttacctg
      301 ttgtgcaata aggcgtttag cgtgctgtcg accaatgccc caaacatctt caattttcaa
      361 ccatttcaac gctttgatgc gtttttcctc gctgtctata acataaacgc cattggtgcg
      421 ctctggaaat tttttcgcaa ttcggttggc agctttagca agtgctttgg tgggggcaat
      481 gccaacactg atggggattc ctgtccattt accaactttt tttcgcatgt tcaacccgta
      541 atcgttgagg ttgaaatacg tatcaaatcc gtgcaatttc aaaaaacatt catcgatgct
      601 gtaaatttcc atttcgggtg agtaatcgga gagaatggtc atcacgcggt ttgacatgtc
      661 gccatacaac gcaaaattgg tagaaaaaac gtgcacatgg tgatgcttaa acatgttttc
      721 gtattcaaat gcggcggctc ccatcggaat accaagtgct tttgcttcgt tgcttcgggc
      781 aataacacag ccgtcgttgt tcgataaaac aacaatgggt tttccgttca aatcaggtcg
      841 aaaaacacgt tcgcacgaag cgtaaaaatt attgcagtca atcagagcaa acatatcaaa
      901 ggtcttttat aacatgggta acaattcccc aaatcagaaa atttgtgtct tcggtaatgc
      961 gaatgggttg gtaattttcg ttttcgggaa cgagccaaag ttcatttttt gcaaggcgaa
     1021 tgcgttttgc ggtaaattct ccatcgatgt agcacaccgc tatttttcca ttggcaggct
     1081 ccaaactccg atcgatgatt aacaagtcgc catcaccaat gcccgcattt ttgagcgata
     1141 cccctttaac acgtccataa aaagtggctg taggatgttt gatgaggtgc ttgtttaaat
     1201 caatggtgag gtcaataaaa tccaatgcag gagaggggaa cccggcgcta attccgtcag
     1261 acacataggg caaatgcagt cgtgttgccg tatttgcgtt aaacaattca accacagagt
     1321 ggggatgcga ttctaaaaaa gccattataa atcaattaat tgatgacacg tttgagcaca
     1381 gcatcctaaa tggtactgta cttctttgtt tattatcgct taattgaaat tgttgggaag
     1441 cacaaatata cgaaaaagga atggagaaaa taactgattg gtataaaaac aagaagagaa
     1501 tgattgtttt aaatcggcaa ataccccaca attcattgtt taaaaataaa aaaatggtca
     1561 actgtaaact caaaaaaata gttaactttg taactcgttt taaaattatg gaaaaaacat
     1621 cacaacaaat agcaacagta gagcaggcgg tagaattagg attgcgccaa gacgaatttg
     1681 aaatgatcaa agagatttta ggaagaacac ctaatttcac agaaacagca atttattctg
     1741 ttatgtggtc tgaacactgt tcgtataaaa actctattct ttggttgaaa aaactaccaa
     1801 aagatggtcc acacatgttg gtaaaagccg gtgaggaaaa tgctggatta gtagatatcg
     1861 gtggtggttt gggttgtgca tttaaaatag aatcacacaa tcacccaagt gctttggaac
     1921 cttatcaagg tgctgcaaca ggtgttggtg gtatcaaccg agatattttt acaatgggtg
     1981 cgcgcccagt tgcacaatta aactcattgc gttttggaaa cattgacaga gaccgcacaa
     2041 aatggttggt tagaggtgtt gtaaaaggta ttggcgatta tggaaatgct tttggtattc
     2101 caactgtagg aggtgaagtg ttttttgatg attcgtataa caccaatccg ttagtaaatg
     2161 cattctctgc aggtattttg gattctaaaa aaatcatttc tgcaaaagca aaaggaccgg
     2221 gtaaccctgt gtttattgtt ggttctgcaa caggaaaaga cggaattgcg ggggcagcat
     2281 ttgcttctaa agatattaca gaagaatcgg cac
//
LOCUS       DAOQVQ010000408         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_57954_length_2300_cov_4.699332, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000408 DAOQVQ010000000
VERSION     DAOQVQ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57954_length_2300_cov_4.699332"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKN22_10285"
     CDS             <1..566
                     /locus_tag="PKN22_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS43136.1"
                     /translation="ELVGLLARKHDKFSAYSLGMKQRLAIAAALLNDPEVLILDEPTN
                     GLDPQGIIQIRELIVKIASLGTTIILASHLLDEVEKVCSHVIILEKGKTLYSGKVDEM
                     TANYGYFEINAKDGVALKNWISNHAAFDKMSEEKNHLKVFFKEDVDAATINQLAFEKG
                     IVLNHLVKKKDNLEQQFLTLTQKNYNA"
     gene            563..1393
                     /locus_tag="PKN22_10290"
     CDS             563..1393
                     /locus_tag="PKN22_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS43137.1"
                     /translation="MKRLLAIEWYKLFYYKSTRIFTLLYFALLVVMGVVLAFIEPTIG
                     GVKLDIVQLGFFKFPAFWQNITYLVAIGKMFLAIILILNITNEYANGTLKQNLIDGLS
                     KKEFILSKLYSTLLLVIVSTIFVLGIALVLGASFSGFSAITVQGMDFILAYFVKLVFF
                     FSFCMFLAFLLRKSALSLLGLVVWWVIEAVLRTIESVSLLNSGKIDDQHPFLLTNYLP
                     LGASSNLIGFPKVKIEGFITGQSIFEYQPVDWSYMLVTCLYAILFGWLSYKLIQKRDL
                     "
     gene            1416..2153
                     /locus_tag="PKN22_10295"
     CDS             1416..2153
                     /locus_tag="PKN22_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS43138.1"
                     /translation="MKLIVGLFYFVYKLYVMVLFVVTAVILFPFLTLSTAIKPLHKVA
                     FYLFGFWSIFFRICVLWPPKVRYHEKIKRGAYIIVANHASYGDIFLTPSVLIRFPHVF
                     LGKSEILSYPIIKYYFKNYNIPVFRGDKSKTAKTMALAKEKLAHGWSIVIFPEGGIPD
                     HERPQMLPFKDGAFRLAKETNTPILPVTYVNHYQLFSDPLHWLQSCRPGFAKQVFHPV
                     ITVEEVQQKTVEELKEKCFQLIASGLK"
BASE COUNT          682 a          394 c          438 g          786 t
ORIGIN      
        1 tggaattagt gggattgctt gctcgtaaac acgataaatt cagtgcctat tcattaggga
       61 tgaaacaacg attggcaatt gcagctgctt tgctcaacga cccagaagtg ttgattttag
      121 acgaaccaac caatggcctc gatccacaag ggattattca aattcgggag ttgattgtta
      181 aaattgcatc gttgggtact accattattt tggccagtca cttattggat gaagtggaaa
      241 aagtttgttc acacgttatc attcttgaaa aaggaaaaac gttgtacagt ggtaaagtgg
      301 acgaaatgac cgccaactat ggctattttg aaatcaatgc caaagatggt gttgccttaa
      361 aaaactggat tagcaatcac gccgcatttg ataaaatgag cgaggagaaa aaccacctca
      421 aagttttttt caaggaagat gtggatgcgg caaccatcaa ccaattggcg tttgaaaaag
      481 gaattgtgct caaccattta gtgaagaaaa aagacaattt ggagcagcaa ttcttaacat
      541 taactcaaaa aaactataac gcatgaaacg actattagca attgagtggt acaaactgtt
      601 ttactacaaa tctacccgca tatttacctt gttgtatttt gcattgttgg tggtaatggg
      661 tgttgtgttg gcattcatag aaccaaccat tggcggtgta aaattggata ttgttcagct
      721 tggatttttt aaatttccag cgttttggca aaacattacc tatttggttg caattggaaa
      781 aatgtttttg gccatcatct tgatactaaa cattaccaat gaatacgcca acggcacgtt
      841 aaaacaaaat ttaattgacg gcttgagcaa aaaagaattt atactttcca aactatacag
      901 cacactgctt ttggtaattg tttcaaccat ttttgtttta ggaattgctc ttgtgttggg
      961 tgcgtctttt tctggtttta gtgccatcac ggttcaagga atggatttta ttcttgcata
     1021 ctttgtaaaa ctggtgtttt tcttctcttt ttgtatgttt ttggcgtttt tactccgtaa
     1081 aagtgcactt tcgctcttgg gattggttgt ttggtgggta attgaagctg ttttgcgcac
     1141 cattgaatct gtttcgttgc ttaacagtgg taaaattgac gatcaacatc cgtttttatt
     1201 gaccaactac ttgcctcttg gagcttcgtc taacttaatt ggttttccaa aagttaaaat
     1261 tgaaggtttt attaccgggc aatccatttt tgaatatcaa cctgttgatt ggagttatat
     1321 gctggtaacg tgtttgtatg cgattttgtt tggttggttg tcgtataaat tgattcaaaa
     1381 acgcgattta tagttttaaa aatcactcaa aacgcatgaa attgatagta gggctgtttt
     1441 actttgtata caaactatac gtcatggtgt tgtttgtggt aacagcagtg attttattcc
     1501 catttttaac cctctcaact gccatcaagc ccttgcacaa agtggcattt tatttatttg
     1561 gtttttggag catttttttt agaatttgtg tgctttggcc accaaaagtt cgctatcatg
     1621 aaaaaataaa acgtggagcc tacatcattg ttgccaatca tgcatcgtat ggcgatattt
     1681 ttctcactcc ttcggtattg attcgttttc cacatgtttt tttaggtaaa agcgaaattt
     1741 tatcgtatcc catcatcaag tattatttta aaaattacaa tattcctgtt tttcggggcg
     1801 ataaatccaa aacagctaaa acaatggctt tggcaaaaga aaaattagct cacggttggt
     1861 cgattgttat tttcccagaa ggaggaattc ccgaccacga gcgaccgcag atgttgccgt
     1921 ttaaagatgg agcttttcga ttggcaaaag aaaccaacac ccctatttta cctgttactt
     1981 atgtgaatca ctatcaatta ttttctgatc cgttacattg gttgcaatcc tgtcgtcctg
     2041 gttttgccaa acaagtgttt catccagtca ttacggttga agaagtgcaa caaaaaacag
     2101 tagaggaatt gaaagaaaaa tgttttcaat taattgccag cggattaaaa taatacctca
     2161 caataagaac tggtttagtt aattgctatt aagctaattg ccacaaaaaa agggtgatta
     2221 tcaaaaccac cctttaaact gttgtatctt tttattaaaa ttttaaccaa tactgttttc
     2281 taatttcatt tcaatcaact
//
LOCUS       DAOQVQ010000409         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_58022_length_2298_cov_4.101650, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000409 DAOQVQ010000000
VERSION     DAOQVQ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58022_length_2298_cov_4.101650"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKN22_10300"
     CDS             <1..274
                     /locus_tag="PKN22_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNS43139.1"
                     /translation="IMAAIVLGIGRAIGETMAVLMVTGNAAIIPHSLFDSVRTIPATI
                     AAELGEAPAGGAHYQSLFLLGCILFVITMLISITSELISKKTHKAH"
     gene            276..1124
                     /gene="pstA"
                     /locus_tag="PKN22_10305"
     CDS             276..1124
                     /gene="pstA"
                     /locus_tag="PKN22_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNS43140.1"
                     /translation="MNKKISQKIAFTIFKLFGLIVVGTLFSILGFIFVNGIQVINWEF
                     LTTNPEEGMTSGGIFPAIVGTLCLIIGSLIVAFPLGVMSAIYTNEYAGNGWVVKIIRM
                     MTNNLASIPSIVFGLFGMALFVNKLNFGDSILAGSLTLGILILPIIIRTTEEALKAVP
                     DSYRSGSLALGATKLQTIRRVVLPAATPNIITGLILSIGRVSGETAPILFTVAAYFLP
                     HLPGGIFDQVMALPYHLYVLATSGTDIEAARPMAYGTALVLIFIVLILNLLATLIRRK
                     MGKSKH"
     gene            1151..1897
                     /gene="pstB"
                     /locus_tag="PKN22_10310"
     CDS             1151..1897
                     /gene="pstB"
                     /locus_tag="PKN22_10310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNS43141.1"
                     /translation="MIQAKDINFYYGDFHALKGINMEMERNTVTAFIGPSGCGKSTFL
                     RLFNRMNDLIGNTKLQGECLVNGQNIYHKSVDVDALRKQVGMVFQKPNPFPKSIFENV
                     AYGLRVNQMGDKNFIAQRVEESLVGAALWDEVKDKLHQSAFALSGGQQQRLCIARALA
                     VAPEVILMDEPASALDPISTSKIEELIHELKEKYTIVIVTHNMQQAARVSDKTGFFML
                     GELVEFSETKKMFLNPEKEATQNYITGRFG"
     gene            1931..>2298
                     /locus_tag="PKN22_10315"
     CDS             1931..>2298
                     /locus_tag="PKN22_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNS43142.1"
                     /translation="MKHNEQEMVYLKEEINLMWKLVISQLEKAKTALLENNIGLAHEV
                     ITLEKRVNAFELKIDSNCENYIALYTPVAIDLRLVLSIMKISITLERIGDYAVGIARH
                     VADNDCFENSSNLYDEMELER"
BASE COUNT          713 a          395 c          475 g          715 t
ORIGIN      
        1 aataatggct gccattgttt tgggtattgg tcgtgcaatt ggtgaaacaa tggcggtact
       61 tatggttaca ggcaacgctg caattattcc tcattcactt tttgattctg tgcgcactat
      121 tcctgcaacc attgccgcag aattgggcga agctcctgct ggtggagcac actatcaatc
      181 gttgttttta ttgggatgca tcttgtttgt tatcacaatg cttatttcaa ttacctcaga
      241 gctaatcagt aaaaaaacac acaaagcaca ttaatatgaa taaaaaaata agtcagaaaa
      301 ttgcttttac catttttaaa ctttttgggt taattgtggt gggaaccctc tttagcatcc
      361 ttgggtttat ttttgtaaac ggaattcaag ttatcaattg ggaattttta accactaatc
      421 cagaagaagg aatgacatcg ggtggtattt tccctgcaat tgtaggtacg ctttgtttaa
      481 ttatcggtag tttgattgtt gcttttccgc ttggtgtgat gtcggccatt tataccaatg
      541 aatatgcagg aaatggttgg gtggtaaaaa tcatccgcat gatgaccaac aatttggcaa
      601 gtattccctc cattgttttt ggtttgtttg gaatggcttt gtttgtcaat aaactcaatt
      661 ttggcgattc catcttggct ggatcgttaa ccttggggat tttaatttta cccatcatca
      721 ttcgcaccac cgaagaagct ttaaaagctg ttcctgattc gtaccgttct ggaagtttgg
      781 ccttaggagc aacaaaactg caaaccattc gtcgggttgt tttaccagcg gctactccca
      841 acatcatcac tggtttgatt ttatccattg gacgtgtgtc gggcgaaaca gcaccaattt
      901 tatttacagt tgccgcttat ttcttaccgc atttgcctgg aggaattttt gaccaagtta
      961 tggccttgcc ttatcattta tatgtattgg caacaagtgg aactgacatt gaagcagcca
     1021 gaccaatggc gtatggaact gcattggtgt tgatttttat agtgttaatc ttaaacctat
     1081 tggcaacatt gattcgccga aaaatgggaa aaagcaagca ctaactgaat taaaaaatta
     1141 gaacaaagaa atgatacaag caaaagatat taatttttat tatggtgatt ttcatgcgtt
     1201 gaaaggcatc aatatggaaa tggaacgcaa cacggtaacg gcatttattg gtccgtcggg
     1261 atgtggaaaa tctacttttt tgcgtttgtt taaccgcatg aacgatttga ttggcaacac
     1321 taaattgcaa ggtgaatgtt tggtcaacgg acaaaatatt taccacaaat cggttgatgt
     1381 ggacgcattg cgcaaacaag tgggaatggt gtttcaaaaa cccaatcctt ttccaaaatc
     1441 catttttgag aacgtagctt atggtttgcg tgtaaaccaa atgggcgata aaaactttat
     1501 tgcacaacgt gtagaagaat cgttggttgg cgcagccttg tgggacgaag taaaagataa
     1561 attgcatcaa tctgcatttg cactttcagg aggtcagcaa caacgtttgt gcatcgcacg
     1621 tgcattggca gtagcaccag aagttatttt gatggatgaa ccagcatcgg cattagaccc
     1681 catttcaaca tcaaaaattg aggaattaat tcacgaatta aaagaaaaat ataccattgt
     1741 tattgtaact cataacatgc aacaagcagc acgtgtgagc gataaaactg gatttttcat
     1801 gttgggtgaa ttggtagaat ttagtgaaac aaaaaaaatg tttctcaacc cagaaaaaga
     1861 agcaacacaa aactacatta ctggtagatt tggttaaaaa ccactgtttt tcaaataaat
     1921 aacaaaagaa atgaaacaca atgaacaaga aatggtttat ctgaaagaag agataaatct
     1981 aatgtggaaa ctcgttattt cgcaacttga gaaagcaaaa actgctttgt tggaaaataa
     2041 cattggcttg gcacacgaag tcattacttt agaaaaacgt gtcaatgctt ttgaattaaa
     2101 aatagatagc aattgcgaaa attacattgc attatacact cctgttgcca tcgatttgcg
     2161 tttggtgctt tctattatga aaattagcat cacgttggaa cgaattggtg attatgcagt
     2221 tggcattgct cgtcacgttg ccgacaatga ttgctttgaa aacagcagta atttatacga
     2281 tgaaatggaa ttggagcg
//
LOCUS       DAOQVQ010000410         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_58100_length_2296_cov_4.780455, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000410 DAOQVQ010000000
VERSION     DAOQVQ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58100_length_2296_cov_4.780455"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..2076)
                     /locus_tag="PKN22_10320"
     CDS             complement(268..2076)
                     /locus_tag="PKN22_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS43143.1"
                     /translation="MGPKKRKSEEKIKITKESIQRAKGIFSYLRPYRTTYFIGWIFLV
                     LSTSAGFAFPYLMGQLLGGTSTEPMTMQNSISSINMNNINSVAFALFVLFIGQAVFSY
                     FRVVLFTNATENALRDLKNDAFHKLVYMPMDFFNSNKVGELTNRISNDITQIQETLRT
                     TVAEFFRQAVVVVGGVILLFFFSWKLALIMLSTVPVMAIVAVIFGKFIKKLSKQAQDH
                     AAVSNSVLEEALMGISNVKSFTNELFMFNKFKKSTQEIRDLNVKSGLWRGAFISFIIF
                     CLFGAIVFIIWQGLLLTQGANPELAKGDFFSFVMYTIMMGASVGTLPDMYANIQKAIG
                     ATEHLMVIINDKSEKDSHSGKEKPEITGNVTFENVAFSYPQRPDATVLKNVSFTANKN
                     ETIAFVGQSGAGKSTLASLILNYYSLNGGKILFDGISETDIDLEHLRSHIAIVPQEVL
                     LFAGTIRENILFGNPTASDEQIIEAARQANAYDFISSFPEGLDTQVGDRGIQLSGGQK
                     QRIAIARAILKNPTILILDEATSALDNESERLVQDALDKLMKGRTSFVIAHRLSTIKN
                     ADKIFVLSNGEIVETGNHEELLAQKGIYSGLVEIQG"
BASE COUNT          733 a          447 c          419 g          697 t
ORIGIN      
        1 atccacaaac acaaggcacc agttaaaaat aacgacaatt caaaacctgt tttggcagcc
       61 tcaaaaagtg agttaaccat gttttcaaaa atgtttacat ccccccaaaa ggcaagtttg
      121 cacagtgcaa caacaaatgg aataagaaac attccgatga aaatacgatt caataacata
      181 ctacaaatat gtcggaatta tccactaatt tgagagtgaa agcataaaaa ctataaacac
      241 tggtttattg ataactgaaa accaacctta tccttgaatt tcaaccaatc ccgaataaat
      301 acctttttga gccaaaagtt cttcgtgatt gcccgtttca acaatttcac cattacttaa
      361 aacaaaaatt ttgtcggcat ttttgatggt agataaacgg tgagcaatga caaagctggt
      421 gcgacccttc attaatttat caagggcatc ttgcaccaaa cgttccgatt cattatcgag
      481 tgccgatgta gcttcgtcga gaattaatat ggttggattt ttcaatatgg cacgtgcaat
      541 ggcaatgcgt tgtttttgtc cgccagataa ttgaatacct ctgtcgccta cttgtgtgtc
      601 aagtccctct ggaaatgaag aaatgaagtc ataagcattg gcttgacgag ctgcttcaat
      661 aatttgttcg tcacttgcag ttgggtttcc aaacaaaatg ttttctcgga tagtacccgc
      721 aaacaacaac acttcttgcg gaacaatggc aatgtgtgag cgcaaatgtt ccaaatcaat
      781 atcggtttca ctaatgccgt caaacaagat ttttccaccg ttgagggagt aataattgag
      841 aatcaacgaa gcaagcgtag attttccagc acccgattga ccgacaaaag caatggtttc
      901 attcttattg gcagtaaaag aaacgttttt aagcaccgtt gcatctggtc gttgtggata
      961 ggaaaacgcc acgttctcaa aagtcacatt tcctgtaatc tctggttttt cttttccaga
     1021 atgactgtct ttttcagatt tatcgttgat gattaccatc aaatgttcgg tggcaccaat
     1081 tgctttttga atgttggcat acatgtcagg taacgtaccc acagaagcac ccatcatgat
     1141 ggtgtacata acaaacgaaa agaaatcgcc ttttgccaat tctgggtttg cgccttgcgt
     1201 taacagcaat ccttgccaaa tgatgaaaac gatggcccca aacaaacaaa aaatgatgaa
     1261 cgagatgaat gcaccacgcc acaaaccact ttttacgttt aaatctctta tttcttgcgt
     1321 cgattttttg aatttattga acatgaacaa ctcattggta aaggatttta cgtttgaaat
     1381 gcccatcaat gcttcttcta aaaccgaatt gctaactgct gcatggtctt gcgcttgttt
     1441 ggataatttt ttgatgaatt taccaaaaat aacggctaca attgccataa caggaacggt
     1501 agaaagcata attaaagcca atttccatga gaagaaaaag agtaaaataa caccgcccac
     1561 cacaacaact gcttggcgga aaaactcggc aacagtagtg cgcaatgttt cttgaatttg
     1621 ggtgatatcg ttggaaatgc gattggttaa ctccccaact ttattggagt taaagaaatc
     1681 cattggcatg taaaccagtt tatgaaaagc gtcgtttttt aaatcgcgca aggcattttc
     1741 tgtagcattt gtaaataaaa ccactctaaa ataggaaaac actgcttgac caataaacaa
     1801 aacaaaaagt gcaaaagcaa cactgttgat gttgttcatg ttgatggatg aaatggaatt
     1861 ttgcatcgtc attggttcgg ttgatgtgcc tcccaataat tgtcccatca aataaggaaa
     1921 tgcaaaacca gcagacgtgg ataaaactaa gaaaatccaa ccgatgaagt aggtggtgcg
     1981 gtagggacgt aggtagctaa aaattccctt agctcgttgt atactttctt tggtgatttt
     2041 aattttttct tcgctttttc tttttttcgg tcccatcata gctaacgcac tttttttgcg
     2101 aaacaaaaat actaaaactc agctcaaaag tgtcaaaaga atgtcatttt attccatcca
     2161 aacaatattt gcattggttt gcgataattc ggcttcttta tttttaatga gtgccaaatg
     2221 ttgctcaatg ttttgagttg ctttgtgaag tttcatttgg tttacttttt ttacctaacg
     2281 attcaatgca attgtt
//
LOCUS       DAOQVQ010000411         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_58230_length_2293_cov_3.067918, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000411 DAOQVQ010000000
VERSION     DAOQVQ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58230_length_2293_cov_3.067918"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..667)
                     /locus_tag="PKN22_10325"
     CDS             complement(152..667)
                     /locus_tag="PKN22_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43144.1"
                     /translation="MKHWMKTVALVFLLTALVCGTLTITSNYSFSLMFKITLLSLSGF
                     ICSFAIHKAKYSKLFSGLSILSFGALLFILFNPAQFELFWNYVLVLHILLIGFLVDKT
                     IEAIESSKSFIKLIALISMKITLVLFGIIVLFKLNSNFIFSLLLILLSVSSLLYITAL
                     IVNLKKTKENQ"
     gene            complement(667..1707)
                     /locus_tag="PKN22_10330"
     CDS             complement(667..1707)
                     /locus_tag="PKN22_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HNS43145.1"
                     /translation="MTFDPNGVGIANGNIFGFPVTEDEANIVIIPVPWDATASYGKGT
                     SRGPQAVLDASTQLDFYHPKLDKAYNTKVFMIEISKEWLKVNDRLCKEGCDYIAFLED
                     GGKIEDNTYYQQFLEEINAASDALKNGIKSKAKSVLEQGKIACLLGGEHSTPLGLIEA
                     FDEQNQPFGILHIDAHADLRNAYEGFEQSHASIMFNALKCKNLVRLTQVGIRDIAESE
                     VELINNSNGRVTTFFDWDLKEEQFNGKTWAEQVQTILATLPERVYISFDIDGLSPELC
                     PNTGTPVVGGFKLEQITYLLFELAKSNRTIIGFDLNEVGTSEDSDWDANVGARALWNL
                     VCATELNRQKAK"
     gene            complement(1749..>2293)
                     /locus_tag="PKN22_10335"
     CDS             complement(1749..>2293)
                     /locus_tag="PKN22_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS43146.1"
                     /translation="RGTMYRGKAFKDITYLQLGKFETEDFVNVAKQLQTYNYIDANRI
                     GIQGWSYGGFMSTLAMTKGEGVFKMGIAVAPVTNWKYYDNIYTERFMRTPQENPDGYA
                     DNSPINFAKNLKGNYLLIHGSGDDNVHVQNTMEMINALVAANKQFNSFIYPNRNHGIY
                     GGNTRLHLYTMMLNFVKQNL"
BASE COUNT          722 a          425 c          391 g          755 t
ORIGIN      
        1 caccataggt tgaatcttgg tattgtggat tgcatgtcat ttttaattgt gcagtaaccg
       61 aaccagcaat taaaattgtt aaaaaagtta gtaattgttt tttcatagac tttaatttta
      121 agttaatacg aagatagcaa aagaaaataa attattgatt ttctttcgtt ttttttaaat
      181 taacaatcaa agcagttatg taaagcaatg aagatacact tagtaaaatc agcaataaac
      241 taaaaataaa attgctgttg agtttgaaaa gaacaattat tccaaacaaa actaacgtga
      301 ttttcatgga tattaaggca attaatttaa tgaaggattt tgaactttca atagcttcaa
      361 ttgttttatc gaccaaaaaa ccaatcaaca aaatatgaag tactaacaca tagttccaaa
      421 acaattcaaa ttgtgctgga ttgaataaaa tgaaaagcaa agctccaaag gagaggatgg
      481 aaagcccact aaaaagttta ctgtattttg ctttgtgtat ggcaaaagaa caaataaaac
      541 cagataatga aagcaacgtg attttgaaca tcagagaaaa actgtaattt gaagtgatgg
      601 ttaatgttcc gcaaacaagg gctgtaagca gaaaaaccaa cgcaacagtt ttcatccaat
      661 gtttcattat tttgcctttt gacggttgag ttctgttgcg cacaccaagt tccacaatgc
      721 acgtgcacca acgtttgcat cccaatcaga atcttcactt gttccaactt cgtttaagtc
      781 aaaaccaata attgttcggt tcgattttgc caattcaaac aacaaatagg tgatttgctc
      841 caatttgaat ccgcccacaa ctggcgttcc ggtattagga cacaattctg gactcaaccc
      901 atcaatgtca aacgagatgt acacacgttc tggcaaagtt gccaaaatgg tttgcacttg
      961 ctctgcccac gtttttccgt tgaattgttc ttctttcaaa tcccaatcaa aaaaggtagt
     1021 tacgcgtcca ttgctattgt tgattaactc tacttctgac tcggcaatat cgcgaattcc
     1081 aacttgagtt aaacgcacta aattcttgca ttttaaagcg ttgaacataa tacttgcgtg
     1141 cgattgttca aacccttcgt aggcatttct taagtcggca tgggcatcaa tgtgtaaaat
     1201 tccaaacggt tgattttgct catcaaaagc ttcaattaat cccagtgggg tagagtgctc
     1261 acctccaagc aagcaagcaa ttttaccttg ttctaaaacc gactttgctt tggatttaat
     1321 tccgtttttt aaagcatcag atgctgcatt aatttcttct aaaaattgtt ggtaataggt
     1381 gttgtcttca atttttccac catcttccaa aaacgcaatg taatcacaac cttctttgca
     1441 caaacgatcg tttactttta accattcttt agaaatttca atcatgaaaa cttttgtgtt
     1501 gtaggctttg tctaatttag gatggtaaaa atccaattga gtggaagcat ccaacacggc
     1561 ttgtggtcct ctacttgttc cttttccata cgaagctgtt gcatcccaag gaacgggaat
     1621 aatcacaatg tttgcttcat cttctgttac tggaaatcca aaaatatttc cgttggcaat
     1681 tcctacgccg tttggatcaa atgtcatatt ctaattttaa agctgcaaat ttagtttttc
     1741 cgatgaattt agaggttttg ttttacaaaa ttgagcatca tggtgtataa atgcaaacgt
     1801 gtattgcccc cataaattcc atggtttcta ttgggataaa tgaaagaatt aaattgtttg
     1861 ttggctgcca ctaaagcatt aatcatttcc attgtgtttt gtacatgcac gttgtcatcg
     1921 cctgaaccgt gaatgagcaa gtagttacct tttaggtttt ttgcaaaatt tataggtgaa
     1981 ttatcagcat aaccatccgg attttcttga ggtgtgcgca tgaaacgctc ggtgtaaata
     2041 ttgtcgtaat atttccaatt ggttaccgga gcaacagcaa ttcccatttt aaaaactcct
     2101 tcgcctttgg tcatagcaag tgttgacata aatccaccat agctccaacc ttgaatacca
     2161 atgcggttgg catcaatgta gttgtaggtt tgcaattgtt tggcaacgtt tacaaaatct
     2221 tcggtttcaa attttcccaa ttgtaaatag gtaatgtctt taaatgcttt tccacgatac
     2281 atggtgcctc ttg
//
LOCUS       DAOQVQ010000412         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_58823_length_2278_cov_3.217724, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000412 DAOQVQ010000000
VERSION     DAOQVQ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58823_length_2278_cov_3.217724"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKN22_10340"
     CDS             complement(<1..778)
                     /locus_tag="PKN22_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009640671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNS43147.1"
                     /translation="MDWRGRRKSDNVEDRRGISGGKMAMGGGLIGLAIYFIVQFMGGD
                     ANMASQISSQLSNQIGNQTETTRELTPEEKEIGDFVATVFGDTEDVWAKLLQNTKTPY
                     RYPKMVLFTGQVATKCGNASSAVGPFYCPGDEKVYMDLDFFNELKTKFGAKGGDFAIA
                     YVIAHEVGHHVQHVMGTTAAVRQAQQGKSEKEQNKLSVALELQADFYAGVFAHYINNY
                     LDADDIDEALSAVQAVGDDAIQKKMQGHVNPDSFTHGTSAQ"
     gene            1004..>1618
                     /locus_tag="PKN22_10345"
     CDS             1004..>1618
                     /locus_tag="PKN22_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43148.1"
                     /translation="MKKVLIIAYDFPPYVSVGGLRPYSWYKYMKEFGVEPIVVTRQWS
                     NKHGNALDYISASESKETLIEQTEYGTIIRAPYFPSISNRILLKYGEQKFTFFRRALT
                     AWDEFRQFITISGPKKHLFHAANDYLKHNKVDAIIATGDPFITFYFAKKLGAKYNVPW
                     IADYRDPWSQNLKKQNSIYKFWINSLEKKIVSASALITTVPSINS"
     assembly_gap    1619..1628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1629..>2278
                     /locus_tag="PKN22_10350"
     CDS             <1629..>2278
                     /locus_tag="PKN22_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43149.1"
                     /translation="FIKIVKKNVVSTATSITTVSQFLKKKITQNLKHKIFHILPNGYD
                     QSLTEIVENLPQKSDKLTIAFAGTIYQWHPWKSFLSVCSHLIEHHNIHVHLQFYGLNN
                     PIEFNNYILTLPIKTQNSISITPKLQNKELLQQLAQCNLMLLFNDYSILGTKIYDYLG
                     IKRKIILCYKNDTQALQLKNKYYNIEEVEGVSKTLQEDLIKETNAGIVVENEEHLK"
BASE COUNT          776 a          452 c          338 g          702 t
ORIGIN      
        1 gttgtgcaga tgttccatgt gtaaacgagt ctggattcac atgtccctgc atttttttct
       61 gaattgcatc gtcgccaaca gcttgtacag cactcaatgc ctcatcaatg tcatctgcat
      121 ccaaataatt attgatgtaa tgcgcaaaaa ctcctgcata aaaatcggct tgtaactcaa
      181 gggcaaccga caatttattt tgttcttttt cagatttccc ttgttgcgct tgtctaactg
      241 ccgccgtggt tcccatcaca tgctgcacat gatgccccac ctcgtgtgca attacgtatg
      301 caatagcaaa atcgccccct tttgcaccaa attttgtttt caactcatta aaaaaatcca
      361 agtccatgta cactttttcg tctccaggac agtaaaacgg accaacagcc gagcttgcat
      421 ttccacattt agtggctact tgtccggtaa acaacaccat tttcgggtaa cgataaggcg
      481 tttttgtgtt ttgaagcaat ttagcccaaa catcttctgt atcaccaaaa actgttgcaa
      541 caaaatcgcc aatttctttt tcttcgggcg ttaactcacg cgttgtttcg gtttgattgc
      601 ctatttggtt gctcaactga ctactaattt gacttgccat gttggcatca ccgcccataa
      661 attgaacaat aaaataaatg gccaagccaa taagtccacc acccattgcc atttttccac
      721 cagaaatccc tcttcggtct tccacattat cgctttttcg tcgtcctctc cagtccataa
      781 gaataaatta agttgtatca aatataacaa agatttagca atatcaaata tttggttgat
      841 atgcacatat cgacatattt cgatatcaac aaaatgagat attaaaccca ttatgtttaa
      901 aaacttgcca tttctcgatt gaaattaaat ccttttagcg tatgtttgtt gtgaaaaaac
      961 agaactaaca accaacacaa ccaactgaaa agaatatttg ttaatgaaaa aagtcctcat
     1021 cattgcatac gattttccac cctatgtttc tgtaggaggt ttaaggccgt atagttggta
     1081 taaatacatg aaagaatttg gtgtggagcc aattgttgtt acgcgccaat ggagcaataa
     1141 acacggcaat gcgttggact acatttctgc aagtgaaagc aaagaaactt taattgagca
     1201 aaccgaatat ggtaccatca tccgtgcacc ctattttccg tcaatcagca accgcatcct
     1261 actaaaatat ggtgaacaaa aatttacttt ttttcgaaga gcattaaccg cttgggacga
     1321 atttaggcaa ttcattacca ttagcggacc caaaaaacac ttgtttcacg ctgcaaatga
     1381 ctatttaaaa cacaacaaag tggatgctat cattgcaaca ggtgatccat tcattacttt
     1441 ttattttgca aaaaaattag gagctaaata caacgttcct tggattgcag attatagaga
     1501 tccttggagt cagaatttaa aaaaacaaaa ttctatttat aagttctgga ttaacagtct
     1561 tgaaaaaaaa atagtttcag catctgcatt aatcacaaca gttccctcaa ttaattcann
     1621 nnnnnnnntt cattaaaatt gttaagaaaa atgtagtttc tacagctact agtattacaa
     1681 ctgtgtctca atttttaaaa aaaaaaataa cccaaaattt aaaacacaag atatttcata
     1741 ttcttcccaa tggttatgac caatctttaa ctgaaatcgt tgagaatttg cctcaaaaat
     1801 ctgacaaatt aacaattgca tttgcaggaa caatttatca atggcatcct tggaaaagtt
     1861 ttttatcggt ttgttctcat ttaatagaac atcacaacat tcatgtccac ttgcaatttt
     1921 atggattgaa caaccctatt gaatttaata actacatact tactttacca ataaaaacgc
     1981 aaaacagtat ttctataact cctaaacttc aaaataagga gctccttcag caattagcac
     2041 aatgcaactt aatgcttttg tttaatgatt attcaatttt aggaactaag atttacgatt
     2101 atctaggcat aaaaagaaaa attattttat gttataaaaa cgatacacaa gcacttcaat
     2161 taaaaaacaa atattacaac atagaagaag ttgaaggggt aagtaaaacg ctacaagaag
     2221 acttaattaa agaaacaaac gcaggtatag ttgttgagaa tgaagaacat ttaaaaca
//
LOCUS       DAOQVQ010000413         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_59286_length_2267_cov_3.729656, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000413 DAOQVQ010000000
VERSION     DAOQVQ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59286_length_2267_cov_3.729656"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1074
                     /gene="atpA"
                     /locus_tag="PKN22_10355"
                     /pseudo
     CDS             52..1074
                     /gene="atpA"
                     /locus_tag="PKN22_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            1180..2055
                     /gene="atpG"
                     /locus_tag="PKN22_10360"
     CDS             1180..2055
                     /gene="atpG"
                     /locus_tag="PKN22_10360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNS43150.1"
                     /translation="MASLKEIRNRITSVKSTMQITSAMKMVSAAKLKRAQDAITQMRP
                     YAEKMHEILGNLSATLDISSNAYAAERPVKNVLVIAITSNRGLCGGFNNNVLKRVNQL
                     VNGDLADSNPRILCLGKKAKDAYKKGNFYYVNDVIAPVEDIFSDLTFENASTIANDLM
                     KRFVNKEFDKVVVVYNKFLNAATQDIITEQILPIVKNEQTGGNKNAEYIFEPSKQEIV
                     EDLIPKSLKIQLFKALLDSNAAEHGARMTAMHKATDNANELQRSLTISYNKARQAAIT
                     NEILEIVGGAEALNA"
     gene            2140..>2267
                     /locus_tag="PKN22_10365"
     CDS             2140..>2267
                     /locus_tag="PKN22_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS43151.1"
                     /translation="MSKLCLVPTPIGNLEDITLRAIRYLQEADFILAEDTRVTKKL"
BASE COUNT          785 a          366 c          434 g          682 t
ORIGIN      
        1 tgatcctttt tgaccaacag caacatagat acagtaaaca ggttctcctc tatcataaac
       61 caaaaagaat tttacgatag aggagaacct gtttactgta tctatgttgc tgttggtcaa
      121 aaaggatcaa ctgttgcagg tatccttaaa aaattagaag atgctggagc aatggcgtat
      181 acaactatcg ttgctgctaa cgcttctgac cctgctccaa tgcagttcta tgctcctatg
      241 acaggtgctg caattggtga gtatttccgt gatactggtc gcccagcatt aatcatttat
      301 gatgatttat ctaaacaagc ggtagcttac cgtgaggtgt cattattgtt acgtcgtcct
      361 ccaggtcgtg aggcttaccc aggtgacgta ttctacttac actctcgttt gttagagcgt
      421 gcagcgaaaa tcattaacga tgatgaaatt gcagcaaaaa tgaacgattt accagaatct
      481 ttgaaaggta aagtaaaagg tggaggttct ttgacagcat tgcctatcat tgaaacacaa
      541 gctggtgacg tatctgcata tattccaaca aacgtaattt caattacaga tggacaaatt
      601 ttcttggagt ctgacttgtt caactctggt attcgtccag ctatcaacgt tggtatctct
      661 gtatcacgtg taggtggtaa cgcgcaaatt aaatcaatga aaaaggtagc tggtactttg
      721 aaattagacc aagcacaata ccgtgaattg gaagcgtttg ctaaatttgg ttctgactta
      781 gatgctgcaa ctaaagcagt attggacaaa ggagcaagaa acgttgaaat cttgaaacaa
      841 cgtgaaggtg acccaatgtc ggttgaaaaa caaattgcaa tcatctacgt tggaacaaaa
      901 ggattgattc aaaatgttcc agttgaaaaa gttaaagaat tcgaaaaaga atacttgaac
      961 ttcttagatg caaaacatgc agatgtattg gctcgcttaa aagctggtaa atacgaagac
     1021 gctgatacag atgtgttagc aaaagttgca aaagaaatcg cttctaaata ttaataattt
     1081 gaagtacaaa actaagagaa tgggaatttt ataagtttat tagattccct tctctttata
     1141 taattttaaa ttgtttactt caaattcata attcaaatta tggcaagttt aaaggaaata
     1201 cgtaacagga ttacttcagt gaaatcaacc atgcagatta cttctgcaat gaagatggtt
     1261 tctgcagcaa aattgaaacg tgcacaagat gcaattactc aaatgcgtcc ttatgctgaa
     1321 aaaatgcatg agattttagg taatttgagc gcaactttgg atatttcaag caatgcgtat
     1381 gccgctgaac gccctgtgaa aaatgtattg gttattgcaa ttacatccaa cagaggtttg
     1441 tgtggaggtt ttaataacaa cgttttgaaa agagtaaatc aattggtaaa tggtgattta
     1501 gctgattcaa atccacgtat tctttgtttg ggtaaaaaag caaaagatgc ctacaaaaaa
     1561 ggtaattttt attatgtaaa tgatgtaatt gcacctgttg aagatatttt ctctgattta
     1621 acatttgaaa atgcttcaac tattgcaaat gacttgatga aacgtttcgt taacaaagaa
     1681 tttgataaag ttgtagttgt ttacaataaa ttcttaaacg cagcaacgca agacataatt
     1741 acagaacaaa ttttacctat cgttaaaaac gaacaaacag gtggtaacaa aaatgcagaa
     1801 tatatttttg aaccttcaaa gcaagaaatt gttgaagatt taattccaaa atcattaaaa
     1861 attcaattgt tcaaagcttt gttggattca aatgctgctg aacatggtgc acgtatgacg
     1921 gcaatgcaca aagcaacgga taatgcaaat gagttgcaac gttcattgac tatttcatac
     1981 aacaaagcac gccaagctgc tattacaaac gaaatcttag aaatcgttgg tggagcagaa
     2041 gcattgaatg cataaattaa actagaatca ataaagaatc cctttcaatt tttggaaggg
     2101 atttttattt tttggtaact tagctaaaaa tattagaaca tgtcaaaatt gtgtttggtt
     2161 cctacaccaa ttggaaattt agaagatatt acattaagag ccatccgcta tttgcaagaa
     2221 gctgatttca tcttggcaga agatacgcgc gtaaccaaga agttgtt
//
LOCUS       DAOQVQ010000414         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_59328_length_2266_cov_4.004975, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000414 DAOQVQ010000000
VERSION     DAOQVQ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59328_length_2266_cov_4.004975"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKN22_10370"
     CDS             <1..485
                     /locus_tag="PKN22_10370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNS43152.1"
                     /translation="FNPIGAHPSGLIGELPIGKPNNLLPFITQTAAGLHQELTVFGND
                     YPTPDGTCIRDYIHVVDLAEAHVKGIDFLTSNQPKAVEFINVGTGNGTSVLEMIQAFE
                     ATTGNKLNWKFGPRRDGDVVEIYANADKSFNSLGWKAKYTIQDAIKDAWNWEKNIRNI
                     "
     gene            523..1260
                     /locus_tag="PKN22_10375"
     CDS             523..1260
                     /locus_tag="PKN22_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS43153.1"
                     /translation="MFKQLVIGFLFCAISLNSFGQEKNEVVTKSRYRPGIFWFLTGWK
                     NGKAPKYDRLMFDITYNDWVGDRKTFAMKGPSMGMNVSWLFDIPLAKNGVVSFAIGPN
                     YGLYNLHHNLPVYYDQATKTTSYGENEQAGNFGKIKLVGHQLTVPVEFRFRTKGLQHF
                     KVHIGGKIGYQLSLFNKERHTINGKEEKFKNKDSKDINRLVYSAHVRLGVRNFALYAS
                     YNFNPFYSGESSTKLHLLQLGLTVSLF"
     gene            1262..2038
                     /locus_tag="PKN22_10380"
     CDS             1262..2038
                     /locus_tag="PKN22_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS43154.1"
                     /translation="MFIDTHTHLFSEEFNDDRSLVVKKAIDCGVEKMLLPNIDVNSIH
                     PLFDLCTKFPQNCYAMMGLHPGSVDEHFETQLATIKQALFSNKERCVAVGEIGMDLYW
                     DKTFVEQQRIAFATQINWAKELNIPIVIHAREAFDEIFTVLDELNDERLSGVFHCFTG
                     DEAQAQKALSYGNFKLGIGGVVTYKKSTLPEVLATVDLKHIILETDSPYLAPVPFRGK
                     RNESAYVLHVAEKLADIYQVSLKTIEETTTNNAKELFRIN"
     gene            complement(2031..>2266)
                     /locus_tag="PKN22_10385"
     CDS             complement(2031..>2266)
                     /locus_tag="PKN22_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS43155.1"
                     /translation="VAGVRFLFLGEINNLISVVKAHFSMYANLSSLLQKRRAIKASSV
                     APNTTGRYNGNLLWAYYFKGVKKFSKLNQRLFS"
BASE COUNT          744 a          403 c          447 g          672 t
ORIGIN      
        1 attttaaccc cattggtgcg catccttctg gcttgattgg tgagttaccg attggaaaac
       61 ccaataattt attgcctttt attacccaaa cagctgcagg attgcatcaa gaactaacag
      121 tttttggtaa cgattatcct acacctgatg gcacgtgtat ccgcgattac atccatgtag
      181 ttgatttggc cgaagcacac gtaaaaggaa ttgatttttt aacctccaac caaccaaaag
      241 ctgtagaatt tatcaatgtg ggcactggca acggaacatc tgtattggaa atgattcaag
      301 cgtttgaagc tacgacaggc aataaattaa actggaaatt tggtccgcgc agagatggcg
      361 acgtggtaga aatctatgca aatgccgata aatcgttcaa tagcttgggc tggaaagcaa
      421 aatataccat ccaagatgca ataaaagatg catggaattg ggaaaagaac attcgtaata
      481 tctaaagaat cactaaattt gttgctttat acaattcaat aagtgttcaa acaattagtc
      541 atagggtttc tattctgcgc aatttcactc aatagctttg ggcaagaaaa aaacgaagtt
      601 gtaaccaaat cgcgttatcg accaggtatt ttttggtttt taaccggctg gaaaaatgga
      661 aaagcaccaa aatacgaccg tttgatgttt gacatcacct acaacgattg ggtgggcgac
      721 cgaaaaacct ttgcaatgaa aggaccttca atgggcatga atgttagctg gttgtttgat
      781 attcctttgg ctaaaaatgg tgttgtttca tttgctattg gaccaaatta tggcttgtat
      841 aacctccatc acaatctccc tgtttattac gatcaggcaa caaaaacaac tagctatgga
      901 gaaaatgaac aagctggcaa ttttggaaaa ataaaattgg tggggcatca gttaactgtt
      961 cctgtagaat ttcgtttcag aaccaaaggt ttgcagcatt tcaaagtgca tattggagga
     1021 aaaattggtt atcaattatc actgtttaac aaagaacgcc ataccataaa tggaaaagag
     1081 gaaaagttta aaaacaaaga ttcaaaagac ataaatcgat tggtgtattc ggcacatgtg
     1141 cgcttaggtg ttcgcaattt tgcgctgtat gcttcgtata atttcaaccc attttactcg
     1201 ggagaatcta gcacaaaact tcatttgttg caattaggtt taacggtttc attgttctaa
     1261 catgtttatt gacacgcaca ctcatttatt ttcggaagaa tttaacgacg accgttcgtt
     1321 ggtagttaaa aaagccattg attgtggtgt agaaaaaatg ttgctaccca acattgatgt
     1381 gaattccatc catccgttgt ttgatttatg cactaaattt ccgcaaaact gctatgcaat
     1441 gatgggcttg catcctggtt cggtggatga acattttgaa acgcaattgg caactataaa
     1501 acaagcactt ttttctaaca aagaacgttg tgtggcagta ggtgaaattg gaatggattt
     1561 gtattgggac aaaacatttg ttgaacaaca acgcattgct tttgcaacac aaatcaactg
     1621 ggcgaaagaa ttgaatattc ctattgttat ccatgcgcgc gaggcttttg atgaaatttt
     1681 tacagttttg gatgaattga acgacgaacg cttatcgggt gtatttcatt gttttaccgg
     1741 ggatgaagca caagcacaaa aagcattaag ttatggcaat tttaaattag gaataggcgg
     1801 tgttgtgacc tataaaaaat ctactttgcc agaagtgttg gctaccgttg atttaaagca
     1861 catcatttta gaaaccgatt cgccttattt agcacctgta cccttccgtg gtaaaagaaa
     1921 cgaaagtgcg tatgtgttgc acgtagccga aaaattagcc gatatctatc aagtttcatt
     1981 aaaaacaatt gaagaaacaa ctaccaacaa cgcaaaagaa ttgtttcgaa ttaactaaac
     2041 aaacgttggt tgagtttact aaactttttg acacctttaa aataataagc ccacaacaaa
     2101 ttcccattgt aacgtccagt agtatttgga gctactgaac tagctttaat tgcacgtctt
     2161 ttttgcaata agcttgataa attggcgtac attgaaaaat gggcttttac aacactaatc
     2221 aggttgttaa tttcgcctaa aaaaagaaaa cgaacgccag ccacac
//
LOCUS       DAOQVQ010000415         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_59626_length_2259_cov_5.133394, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000415 DAOQVQ010000000
VERSION     DAOQVQ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59626_length_2259_cov_5.133394"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKN22_10390"
     CDS             complement(<1..732)
                     /locus_tag="PKN22_10390"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNS43156.1"
                     /translation="MENQPKVLMIYTGGTIGMVNDFQTGELKAFDFKHIYNHVPELKE
                     LNVALTTESFSHPIDSSEINIEDWKHLGSIIVDNYEHYDGFVILHGSDTMAYTASALS
                     FMLQGLKKPVILTGSQLPIGTIRTDGKENLFTAIEIAAKKDENNQSLIQEVAVYFEYS
                     LYRGNRSSKVSANQFEAFKSPNFTRLAVAGVTIDYNTEHLYRTTQQELVFFPHFSNEV
                     ALLKIYPGINFSFYQHLFNPTTIKTM"
     gene            complement(811..1698)
                     /gene="fabD"
                     /locus_tag="PKN22_10395"
     CDS             complement(811..1698)
                     /gene="fabD"
                     /locus_tag="PKN22_10395"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNS43157.1"
                     /translation="MKAYVFPGQGAQFSGMGKDLYDQSPKAKALFEKANEILGFDIAK
                     IMFEGTDEELKQTKVTQPAIFLHSTILAATLGEAFKPDMVAGHSLGEFSALVANGTLS
                     FEDGLKLVYKRALAMQKACEAEPSTMAAILGLDDEVVEKICAEIDGVVVAANYNCPGQ
                     LVISGAIPAVEAACAKLSEAGAKRALVLQVGGAFHSPLMEPAREELAAAIEATTFNQP
                     FCPVYQNVNAQGVTNPEEIKKNLVAQLTAPVKWTQIMQQMLADGAKEVVEVGPGKVLQ
                     GLFKKVDRELPTSSAALAE"
     gene            complement(1756..>2259)
                     /locus_tag="PKN22_10400"
     CDS             complement(1756..>2259)
                     /locus_tag="PKN22_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNS43158.1"
                     /translation="TLVLKGHIQLSMVKFPKGTRGVQLDTLARMALWNEGKDYNHGTG
                     HGVGCFMNVHEGPQNIRKDLNPQELLPGMVVSNEPGYYVENKYGIRHENLVVVKEWKK
                     TEWNTFYEFETLTFCPFFKNSIIKELLTAEEIQWLNDYHRQCEEKIGPHLEGDVKNWF
                     LDLVSPL"
BASE COUNT          629 a          476 c          401 g          753 t
ORIGIN      
        1 cattgttttg atagttgttg ggttgaacaa atgttggtaa aatgaaaaat taattccagg
       61 atagattttc aacaaagcca cttcgtttga aaaatggggg aaaaacacca attcttgttg
      121 ggttgttcta tacaaatgtt cggtgttgta atcaatggtt actccagcca ctgctaggcg
      181 tgtaaagttg ggcgacttaa acgcttcaaa ttgattggca gaaaccttag agcttctgtt
      241 gcctctgtaa agcgaatatt caaaataaac cgccacttct tgaatgagcg attggttgtt
      301 ttcgtctttt ttggctgcaa tttcaatggc ggtaaataaa ttttcttttc catcggtgcg
      361 aatggtacca atgggcaatt gcgagcctgt aagaatgacg ggttttttta atccttgtaa
      421 cataaaactc aatgccgatg cggtataagc catggtatct gagccgtgca aaattacaaa
      481 tccatcgtag tgttcgtagt tgtcaacaat gatgcttccc aaatgtttcc aatcttcaat
      541 gttgatttca gaagaatcaa tgggatgact aaaactttcg gtggtaagtg ccacgttgag
      601 ttccttcaac tctggaacat ggttgtaaat atgtttaaag tcaaatgcct ttaattctcc
      661 tgtttgaaaa tcattgacca ttccaatggt gccaccggta taaatcatta aaactttagg
      721 ttgattttcc atatttacat cttattattt aagcaaaaag agtaaaaatt gcttcttact
      781 ctttttgaat tattttttta aactaccgaa ttattctgct aatgcagcgc ttgaagtagg
      841 caattctcta tctacttttt tgaacaatcc ttgcaatact ttaccaggac caacttctac
      901 cacctcttta gcaccatcag caagcatttg ttgcataatt tgcgtccatt ttacaggcgc
      961 agttaattgt gcaaccaaat ttttcttgat ttcctctgga ttggtaactc cttgcgcatt
     1021 cacattttga tacacagggc aaaatggttg gttgaatgta gttgcttcga ttgctgcagc
     1081 caattcttcg cgtgcaggtt ccatcaatgg cgagtgaaaa gcaccaccta cttgcaatac
     1141 caaagctctt tttgcaccag cttctgataa ttttgcacaa gcagcttcca ccgcaggaat
     1201 cgctccagaa attacaagtt gtcccggaca attgtagtta gcagccacaa ccactccgtc
     1261 aatttcagca caaatttttt ctaccacttc atcgtcaaga cctaaaattg cagccattgt
     1321 ggatggttcg gcctcgcatg ctttttgcat tgccaaagca cgtttgtaaa ccaatttcaa
     1381 accatcttca aaactcaatg ttccgttggc aaccaatgca gaaaattcac ccaatgagtg
     1441 accagcaacc atgtctggtt taaatgcttc gcccaatgtt gcagccaata ttgtagagtg
     1501 caaaaagatg gcaggttgcg taacttttgt ttgcttcaac tcttcgtcgg tgccttcaaa
     1561 cattattttg gcaatatcaa atcctaagat ttcattggct ttttcaaata atgcttttgc
     1621 ctttggtgat tgatcgtata aatcttttcc cattcccgaa aattgggcac cttgtcccgg
     1681 aaaaacgtat gctttcatat tgttttctta ttctaattta tcgcgcaaat atagtattat
     1741 tcctcaattg gcattttaga gtggacttac caaatccaaa aaccaatttt tcacgtcgcc
     1801 ttctaaatga ggaccaattt tttcctcaca ctgacggtga taatcgttca gccattgaat
     1861 ttcttcggct gttagcaatt cttttataat ggaatttttg aaaaacggac aaaaagtcaa
     1921 tgtttcaaat tcatagaatg tgttccattc tgtttttttc cattctttaa ctactacaag
     1981 gttttcatga cgaataccgt atttattttc aacataataa cccggctcgt tagaaacaac
     2041 catgcccggc aacaactctt gcggattgag gtctttgcga atgttttgcg gtccttcgtg
     2101 gacgttcata aagcaaccaa caccatgtcc tgttccatga ttgtaatcct tgccttcgtt
     2161 ccacaaagcc atgcgtgcca atgtatccaa ttgaactcct cttgtgcctt ttggaaattt
     2221 taccatcgaa agttgaatgt gtcctttcaa caccagcgt
//
LOCUS       DAOQVQ010000416         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_59713_length_2257_cov_4.073115, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000416 DAOQVQ010000000
VERSION     DAOQVQ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59713_length_2257_cov_4.073115"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2061)
                     /gene="recQ"
                     /locus_tag="PKN22_10405"
     CDS             complement(<1..2061)
                     /gene="recQ"
                     /locus_tag="PKN22_10405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNS43159.1"
                     /translation="MENLVDVDLKGALKKYFGFDQFKGKQESIVTNLLEGKNTFVIMP
                     TGGGKSLCYQLPALISEGTAIVVSPLIALMKNQVDAIRNVSENEAVAHFLNSSLTKTE
                     ITQVKADISKGVTKLLYVAPESLTKQENIDFLTSVNISFFAIDEAHCISEWGHDFRPE
                     YRRLREIFECISNVPIIALTATATPKVQYDIQKNLNMLDATVFKSSFNRDNLYYEIRP
                     KRDIEKQIIKYIRSKEGKSGIIYCLSRKKVEELAELLQVNGINALPYHAGLDANTRAK
                     HQDMFLMEDADVIVATIAFGMGIDKPDVRFVIHHDIPKSIESYYQETGRAGRDGGEGD
                     CLAFYQYKDIEKLEKFLQGKPVAEQEIGKQLLSEIVSYAETSVCRRKFILYYFGEDFN
                     ESNCNCNCDNCKNPKERFEGKDYVLDLLNVIKELGEVQKAKHICSFITGKVTSDIKSY
                     KHDALENFGIGKEKDEHFWNAVIRQTLVGGLIYKEIESYGVVKLTDKGKEFIQNPTSF
                     KLIKEHDYSDEDSEDIINSGKGAVLDEVLYSQLTDLRKTISKQKNIPPFVIFQEPSLR
                     DMCFQYPITIDELTNIQGVGNGKAAKFGAPFVALIKAYVEENDIERPQDLVVKSLINK
                     SGLKVQLIQNIDRKLPLEDIGRSQGKTIDEIIDEIEAIVASGTRVNIDYYIDEILDPE
                     NQEEI"
BASE COUNT          712 a          415 c          334 g          796 t
ORIGIN      
        1 aatttcttct tggttttctg ggtcaagaat ttcatcaatg tagtaatcaa tattgacacg
       61 tgttccagaa gcaacaatag cttcaatctc atcaattatt tcatcaattg ttttaccttg
      121 tgaacgacca atgtcttcga gtggaagctt gcggtcaatg ttttgaatca actgaacttt
      181 taaaccactt ttattgatga gtgatttaac aaccaaatct tgtgggcgct caatatcatt
      241 ttcttcaaca taagctttta ttaatgccac aaatggagca ccaaacttcg cggctttgcc
      301 attgccaacc ccttgaatat tcgtcaattc atcaatagtg atgggatatt gaaaacacat
      361 atcacgaagt gaaggttctt ggaaaataac aaatggagga atattctttt gtttagaaat
      421 agtttttctt aaatccgtaa gttgagaata aagtacttca tctaaaacag ctcctttgcc
      481 actattgata atatcttctg aatcctcatc tgaataatcg tgctctttga ttaacttaaa
      541 tgaagtagga ttttgaatga attcttttcc tttatcagta agttttacta caccatacga
      601 ttctatttct ttataaatca agccaccaac aagtgtttga cgaataacag cattccagaa
      661 atgttcatct ttttcttttc caatgccaaa gttttcaagt gcatcgtgct tgtatgattt
      721 aatgtccgat gtaactttac ctgtaataaa tgaacaaatg tgcttagctt tttgtacttc
      781 gcctaattct ttaataacgt ttaataagtc aagcacataa tccttccctt caaatcgttc
      841 ttttggattt ttacaattgt cgcaattgca attacaatta ctttcgttga aatcttcacc
      901 aaaataatac aaaataaact ttctgcgaca tacagatgtt tcagcataag atacaatctc
      961 ggacaacaat tgcttgccaa tttcttgttc tgcaacaggt tttccttgta aaaatttttc
     1021 taacttttca atgtctttgt attggtaaaa tgccaagcaa tcgccttcac cgccatctcg
     1081 accagcgcga ccagtttctt ggtaataact ttcaatagat tttggaatat cgtggtgaat
     1141 tacaaaacgc acatctggtt tgtcaatacc cataccaaag gcaattgttg caacaataac
     1201 atcggcgtct tccatcaaaa acatgtcttg gtgttttgca cgagtattag catctaaacc
     1261 agcgtgatag ggaagggcat taataccgtt tacttgcaat agctcggcta attcttccac
     1321 ttttttacga ctcaagcaat aaataattcc actttttcct tctttggaac gaatgtattt
     1381 aataatttgc ttttcaatat cgcgttttgg tcggatttca taatacaaat tatcacggtt
     1441 aaacgaagat ttgaaaacag tcgcatccaa catgttcaaa ttcttttgaa tgtcgtattg
     1501 aacttttggt gttgctgttg ccgttaaagc aatgattgga acattggaaa tacattcaaa
     1561 aatctcacgt aatcttctgt attctgggcg aaaatcgtgt ccccattcag aaatacagtg
     1621 tgcctcatca atcgcaaaaa aggaaatatt gactgaagtt aaaaaatcaa tattttcttg
     1681 tttggtcaac gattctggtg ctacgtatag tagttttgta accccttttg aaatatcagc
     1741 tttaacttgt gtaatttctg ttttggtcaa tgatgaattt aaaaaatggg caacagcttc
     1801 gttttcactc acatttctta ttgcatccac ttgatttttc atcaaagcaa ttaacggtga
     1861 aacaacaatt gcagttccct cagaaattaa tgcaggtaat tgataacaga gcgattttcc
     1921 accacccgtt ggcataatta caaacgtatt ttttccttcc aataagttag taacaataga
     1981 ttcttgcttt cctttgaatt ggtcaaatcc aaaatacttt ttcagtgcac cttttaaatc
     2041 tacatctacc aagttttcca tataataact gatgatttta ttactttcct acgaaaatac
     2101 tacaaaaaat agttttaaaa aaataatttt tggaaataat aataaccaat tttggaatct
     2161 ttactgaaaa ttagaaagat aacgcttcat tattttgtca gaataatttt attagtggaa
     2221 gatttcaaga atttcgtaac ttttgaattg caaatag
//
LOCUS       DAOQVQ010000417         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_59905_length_2253_cov_3.272975, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000417 DAOQVQ010000000
VERSION     DAOQVQ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59905_length_2253_cov_3.272975"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /gene="nrfD"
                     /locus_tag="PKN22_10410"
     CDS             <1..1063
                     /gene="nrfD"
                     /locus_tag="PKN22_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNS43160.1"
                     /translation="EAMTIFAVFMAGIFPLIHMGRLWVGYFVMPIPNQFGSLWVNFNS
                     PLLWDVFAISTYLSVGFVFWYIGLIPDFATLRDRAKKPVSKKMYSILSFGWSGRAKHW
                     NRFELVSLVLAGLATPLVFSVHSIVSFDFATSVIPGWHTTIFPPYFVAGAVFSGFAMV
                     QTIMLILRKAMNLEAYLHTKHIEYMHIVIMVTGSIVGTAYITELFVSWYSGVEYEGYA
                     FFNRATGHYWWAYAIMMTCNVVSPQLMWFKKLRRSMLFTFIISIVVNIGMWFERFVII
                     VSSLHRDYLPSSWSMQYPTHIDIGIYIGTIGLFFTFYLLFARFFPVMPIAELKTILKI
                     SGESYKNAPDTHHGHDEKH"
     gene            1084..1620
                     /locus_tag="PKN22_10415"
     CDS             1084..1620
                     /locus_tag="PKN22_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNS43161.1"
                     /translation="MAEKVIYAMYDDDDVLKDGAKKLVAKGIKVADVFSPFPIHGIDP
                     IIGVKNTRLGIMSFLYGLTGLTLATIGMRYFMIVDWPMNIGGKPSFSYLENMLAFIPI
                     TFEFTVLCAAHFMALTYLIINRTLPGMPARNPDPRTTDDKFVMEIRTSDNAKFTYEEI
                     ESMLKETGIIELDQKEVK"
     gene            1660..>2253
                     /locus_tag="PKN22_10420"
     CDS             1660..>2253
                     /locus_tag="PKN22_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS43162.1"
                     /translation="MKMNFKAIVSLSLTVVALSSCVTDPDSSGYEYMPDMYRSAAVEP
                     YVDYGQVKERVNPEVAGKLSAMVPPFGAIPYFGTDSATVAINMPFPILPNSAFKLTHD
                     LRGFAFSDEDTYKLAANYTTNPLKLTPENAEEIFAKGKVLFNNNCAQCHGEKGDGKGT
                     MVTNNIFTGVPDYADKKDLSNGQMFYSIYYGKGAMGSH"
BASE COUNT          692 a          368 c          443 g          750 t
ORIGIN      
        1 agaggcgatg actatctttg ccgtattcat ggcaggtatt ttcccattga ttcacatggg
       61 acgtctttgg gtaggttatt tcgtaatgcc aattccaaac caattcgggt ctttatgggt
      121 taactttaac tctccgttgt tatgggacgt atttgcgatt tctacttatt tatcagtagg
      181 tttcgtgttc tggtacatcg gtttgattcc tgactttgca acattacgcg atagagctaa
      241 aaaacctgtt tctaaaaaaa tgtattctat cctttctttt ggatggtcgg gtagagcaaa
      301 acactggaac cgttttgaat tggtttcatt agttttagca ggtttggcta caccacttgt
      361 attctcagtt cactctattg tatcatttga ctttgctact tcagtaattc caggatggca
      421 taccacgatt ttccctccat acttcgttgc tggtgcggta ttctctgggt ttgcgatggt
      481 acaaacaatc atgttgattt tgcgtaaagc aatgaactta gaagcttatt tgcatacaaa
      541 acacattgag tacatgcaca tcgtaatcat ggtaactgga tctatcgtag gtacagctta
      601 tatcactgag ttattcgttt catggtattc aggtgtagaa tatgaaggat atgcattctt
      661 taaccgtgct acaggtcact attggtgggc atacgctatc atgatgacat gtaacgtggt
      721 ttctcctcag ttgatgtggt tcaaaaaatt gagaagaagt atgttgttta cattcattat
      781 ttcaatcgta gtaaacatcg gtatgtggtt tgagcgtttt gtaattatcg tatcatcatt
      841 acaccgtgat tatttaccat cttcatggag tatgcaatac ccaactcata ttgatatcgg
      901 tatttacatt ggaactattg gattgttctt cacgttctac ttattatttg cacgattctt
      961 cccagtaatg ccaatcgcag aattgaaaac aattttgaaa atttctggtg aatcttataa
     1021 aaatgctcct gatacacatc acgggcacga tgaaaaacat taataacttt ataattaaag
     1081 gcaatggcag agaaagtaat atatgcaatg tatgatgatg acgacgtttt aaaagacggc
     1141 gcaaaaaaat tggttgcaaa aggtataaaa gtagcagatg tattttcacc gttcccaatt
     1201 cacggaattg atcctatcat aggagtgaaa aatacacgtt taggaattat gtcgttctta
     1261 tatggtttaa caggtttaac acttgcaact atcgggatgc gttatttcat gattgttgac
     1321 tggccgatga atattggtgg taaaccaagt ttttcatact tagaaaatat gttggcattt
     1381 attccaatta catttgagtt tactgtattg tgtgcggctc acttcatggc gttaacgtat
     1441 ttgattatca atagaacgtt gccaggaatg ccagcaagaa atccagatcc tcgtactaca
     1501 gacgataaat tcgtaatgga aattagaact agtgataatg ctaagtttac atacgaagaa
     1561 attgaaagta tgctaaaaga aactggtatt attgaattgg accaaaaaga agttaaataa
     1621 ttgctgattt tcgaaaaatc gaaataaaaa ttagaaaaaa tgaaaatgaa ttttaaggca
     1681 atagtaagtt tatcattgac agtcgtagca cttagttctt gtgttaccga ccctgatagc
     1741 tctggttatg aatacatgcc cgatatgtac cgttcagctg cagttgaacc gtatgtagat
     1801 tacggacaag ttaaagaacg tgtgaatcct gaagtagctg gtaagttatc agcaatggta
     1861 cctccttttg gagctattcc ttatttcggt acagattcag caacagttgc aatcaatatg
     1921 ccttttccta ttttgccaaa tagtgcattt aagttaacgc atgatttacg tggatttgca
     1981 ttttctgatg aagatacata taaattagct gcaaattaca caaccaatcc tttgaagtta
     2041 actcctgaaa atgcggaaga aatttttgca aaaggtaaag tattgttcaa taacaactgt
     2101 gctcaatgcc atggcgaaaa aggtgatggt aaaggaacaa tggttacaaa taatatcttt
     2161 acaggtgtcc ctgattatgc agacaagaaa gatttgagta atggacaaat gttctattca
     2221 atttattatg gtaaaggtgc aatgggttca cat
//
LOCUS       DAOQVQ010000418         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_60107_length_2248_cov_3.843137, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000418 DAOQVQ010000000
VERSION     DAOQVQ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60107_length_2248_cov_3.843137"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>2248)
                     /locus_tag="PKN22_10425"
     CDS             complement(223..>2248)
                     /locus_tag="PKN22_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS43163.1"
                     /translation="AVKVINNNYDMYSAKEKGAPSDAYYHLARCYHLDEQLDKAIEYY
                     NLFINNSRKKSELMDNAKLGITQCNLAKQVIANPKSAKVVNVGSKINTEYPEYSPFIS
                     FDGKSLYFTSRKPWADSSTHEFRDPKLYQFPEDIYVSYLSKGEWTDPKKMDFCEPRTN
                     EATIAVSVDERRVYSYEDATGNGDIYYSDFVKDNFTTFAQIDAPNVNTNFWETHCAVT
                     PDGKNIYFVSDRPGGFGGRDIYRIVKMADGSWSEPKNLGAKINTAYDEDSPFIAIDNK
                     TMYFASNGPNSIGGFDIFVSVRDDNNEWSDPINLGYPINSTGDDLFYTTTVNGKKGYL
                     SSFRKGGYGEKDLYEIENDYMGVQNVIVLKGNIHVLGNAVLTEAAHVVLKCENCVEEK
                     ESVVSLRMRDGAFMTPLEACMDYEIIFMEDSSKVIRREKFNTSCNKKYEEITKDAYIA
                     DYDLLGSVSDKETKEPIKDVTVKVYDKKTNKLLETLTTDEAGKFLSTLLDDKLYGDTI
                     SYELKFSKPGYLTITRDVDQILATNIKIDEVITLEKTDIGKDLAEMISLNPIYFDLDK
                     WDIRPDAEIELNKIVRIMNDNPNIRIELGSHTDCRATKAYNLTLSDKRAKSSAEYIRK
                     RITNPSRIFGKGYGESKLLNDCGCEGDVVSDCTEEEHQLNRRTEFRIVKQ"
BASE COUNT          689 a          422 c          324 g          813 t
ORIGIN      
        1 tgtggggacc acgtccacaa gatgctcaaa attttgttgt aaattcaaaa gaacaaggta
       61 ttccaatgga acaaatcaaa attgatattc aaaattggta caatgccaat aaaggcgaag
      121 ccattcaaaa agaattagtg gaattgatat aaatttcgtc caaaatgaaa aatcccgctt
      181 tagatttcta aagcgggatt ttttattgat aaacattcaa tcttattgct taacaattct
      241 aaattctgta cgtctgttta actgatgttc ttcttctgta caatcagaaa ctacatcacc
      301 ttcacaacca caatcgttca acaattttga ctcaccatat cctttaccaa agattcttga
      361 aggattagta atacgttttc tgatgtattc agctgatgat ttagcacgtt tatcagatag
      421 cgtcaagttg tatgcttttg ttgcacgaca gtcggtatga gaccctaatt caatacgaat
      481 attaggattg tcgttcatga ttctaacaat cttattcaac tcaatttcag catctggacg
      541 aatatcccat ttgtctaaat caaagtaaat aggatttaaa gaaatcattt cagctaaatc
      601 tttaccaata tctgttttct ctaacgtaat tacttcatca attttaatgt ttgtagccaa
      661 aatttgatct acatctctcg taattgttaa gtaaccaggt ttagaaaact tcaattcata
      721 actaatcgtg tcgccataca atttatcatc caacaaggta ctcaagaatt tacccgcttc
      781 atctgttgtt aaagtttcca ataatttatt agttttctta tcgtagactt taaccgtaac
      841 atctttaatt ggttctttgg tttctttgtc acttacacta cccaataaat cgtagtcagc
      901 aatgtatgca tcttttgtaa tttcttcgta ttttttatta caagaagtgt tgaatttttc
      961 acgtctgata acttttgagc tatcttccat gaaaataatt tcatagtcca tacaagcttc
     1021 caatggtgtc ataaatgctc catcgcgcat acgtaaagaa actacacttt ctttttcttc
     1081 cacacagttt tcgcatttta atacaacgtg agcagcttct gttaatacgg catttcctaa
     1141 tacgtggatg tttcctttta atacaattac gttttggaca cccatgtaat cattttcaat
     1201 ttcatacaag tctttttcgc catacccacc ttttctaaac gatgataaat aacctttttt
     1261 accatttaca gttgtggtat aaaacaaatc atcacccgtt gagttaatag gataacctaa
     1321 gttaataggg tcagaccatt cattgttgtc atctctaaca gatacgaaaa tgtcaaaacc
     1381 accaatacta tttggtccat ttgatgcaaa atacatggtt ttgttgtcaa tggcaatgaa
     1441 aggagaatct tcatcgtatg cggtatttat ttttgcgcca agattttttg gttcactcca
     1501 agatccatca gccattttta caattctgta gatatctcta cctccaaaac ctcctggtct
     1561 atctgaaaca aaataaatat tttttccatc tggagttact gcacagtgtg tttcccagaa
     1621 atttgtgttt acatttggcg catcaatttg tgcaaaagtt gtgaagttgt cttttacaaa
     1681 atcagaatag taaatatctc catttccagt tgcatcttca taagagtaaa ctctgcgttc
     1741 atcaacacta acagcaatgg ttgcttcatt tgttctgggt tcacaaaaat ccattttttt
     1801 tggatctgtc cattctcctt ttgataaata acttacataa atatcttctg ggaattgata
     1861 caatttaggg tctctaaatt cgtgtgttga actatcggcc catggttttc ttgaagtaaa
     1921 atacaacgat ttaccatcaa aagaaataaa tggcgaatat tctgggtatt cagtattgat
     1981 tttagaacct acattaacaa ctttagcact tttaggattg gcaatcactt gttttgctaa
     2041 gttacattgc gttattccta attttgcatt gtccattaac tccgattttt ttctactgtt
     2101 gttaataaac aaattgtaat actcaattgc tttgtctagt tgctcgtcca aatgataaca
     2161 acgtgctaag tggtaatatg catcagaagg tgctcctttt tcttttgcag aatacatatc
     2221 gtagttgttg ttaattacct taacagct
//
LOCUS       DAOQVQ010000419         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_60294_length_2244_cov_3.657835, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000419 DAOQVQ010000000
VERSION     DAOQVQ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60294_length_2244_cov_3.657835"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..635
                     /gene="cmk"
                     /locus_tag="PKN22_10430"
     CDS             3..635
                     /gene="cmk"
                     /locus_tag="PKN22_10430"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNS43164.1"
                     /translation="MAKDVAAALNYVFIDSGAMYRGITLYAMQNNLIQNGEVLKNELI
                     EQLHQINIHFEKNGSGKDCLFLNTVNVEDEIRKPAVAELVSKVATIKEVRQKLVQLQR
                     EIGKNGGIVMDGRDIGSVVFPHAEVKLFVTADNSVRAQRRLDELKEKGIKTSLEEVLK
                     NLEERDEMDRTRKESPLIQTDDAILIDTTHHTRESQLKHVLEIVHQKMAV"
     gene            672..>2244
                     /gene="uvrC"
                     /locus_tag="PKN22_10435"
     CDS             672..>2244
                     /gene="uvrC"
                     /locus_tag="PKN22_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009745653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNS43165.1"
                     /translation="MDFSKDLLEKVKLLPNKPGVYQYFNEKNEIIYVGKAKDLKKRVT
                     SYFSKTQDNGKTRLLVRSIRDIRYIVVDTELDALLLENNLIKEYQPKYNIQLKDDKTY
                     PWICIKKEPFPRVFTTRNVVKDGSTYYGPYSSVKVINTLLELIRDLYPLRTCTLDLSA
                     NKIAEKRYKPCLEYFIGNCLAPCVGKQTLEAYNQQIEAIEMILKGNTASTINLLKKQM
                     HDFAASYAFEEAQKVKERLEIIQRFQAKSTIVSPKIHEVDVLTMVEDDKSRFVNYLVI
                     HKGAIVHGLTIEVKTKLDETDEEILNHVLVRMRNQFNSQSKEILAEINFTTEIEHVKI
                     TVPKIGEKKELIELSKRNATYYRIEKRKKETLKNPEKATERILEQIKKDFRLKELPTH
                     IECFDNSNIQGSNPVSACVVFKNAKPSKKDYRIFNVKTVEGPNDFATMEEAVTRRYRR
                     LLDENQPLPQLIVIDGGKGQLSAALKALDEVGLRGKVAIVGIAKKLEEIFFPGDSLPI
                     YIDKRSESLKVIQHLR"
BASE COUNT          803 a          407 c          422 g          612 t
ORIGIN      
        1 ccattgccaa agatgttgca gctgctctca actatgtttt tattgatagt ggtgccatgt
       61 atcgcggtat tacgctctat gcaatgcaaa acaaccttat tcaaaacggt gaggttctaa
      121 aaaacgaatt aattgaacaa ttgcatcaaa taaacattca ttttgaaaaa aacggctctg
      181 gaaaagattg cttgttttta aacacggtga atgtggaaga tgaaatccga aaaccggctg
      241 tagcagaact tgtttctaaa gttgcaacaa taaaagaagt acgtcaaaaa ttagtgcaat
      301 tgcaacgcga aattggtaaa aatggtggca ttgtcatgga tggtcgtgat attggttcgg
      361 ttgtttttcc ccatgcagaa gttaaattat ttgttactgc ggacaacagc gtgcgtgcgc
      421 aacgtagatt agatgaatta aaagaaaaag gcatcaaaac atcgctagaa gaagtactaa
      481 aaaaccttga agaacgcgat gaaatggaca gaactcgcaa ggaaagtcca ctgattcaaa
      541 ccgatgatgc tattcttatc gacaccaccc atcacacccg cgaaagtcag ttgaaacatg
      601 tgctggaaat cgttcaccaa aagatggctg tctaatttgt aaactgctca ttttctctgt
      661 aattttacaa catggatttt tcaaaagatt tattggaaaa agtaaaatta ctgcccaaca
      721 aaccgggagt ttaccaatat ttcaatgaaa aaaacgaaat catctacgtt ggtaaggcaa
      781 aagatttaaa aaaaagggta acttcctatt tttcaaaaac acaagataat ggaaaaaccc
      841 gacttttggt gcgttccatt cgtgatatcc gctacattgt tgtagataca gaattagacg
      901 ctcttttgtt ggaaaacaat ctcattaagg aataccaacc taaatacaac attcaactca
      961 aggacgataa aacttacccg tggatttgca ttaaaaaaga accgtttcca cgtgttttta
     1021 ctacccgaaa tgtagtcaaa gatggctcca cctattatgg cccctactcc agtgtaaaag
     1081 tcatcaatac cttgttggag ttgattcgcg atttatatcc tttgcgcacc tgtaccttgg
     1141 atttaagtgc caataaaatt gccgaaaagc gctataaacc ttgtttagaa tattttattg
     1201 gcaattgctt agctccttgt gtaggcaaac aaaccttgga agcctacaac caacaaattg
     1261 aagccatcga aatgattttg aagggcaaca ccgcttcaac tatcaatttg ctaaaaaaac
     1321 aaatgcacga ttttgcagca tcctatgctt ttgaggaagc acaaaaagta aaagaacggt
     1381 tggaaattat tcaacgtttt caagcaaaat caaccatcgt ttcacctaaa attcacgaag
     1441 tggatgtgtt aacaatggtg gaggacgata aatcgcgttt tgttaattat ttggttattc
     1501 ataaaggagc tatcgttcat ggacttacca tagaagttaa aacaaaactt gacgaaactg
     1561 atgaggaaat tctcaaccat gttttggttc gaatgcgcaa tcaattcaac agccaatcca
     1621 aagaaatttt agcagaaatt aattttacaa ccgaaattga gcatgtaaaa atcacagtgc
     1681 caaaaattgg tgagaaaaaa gaattaatag agttgtcaaa acgcaacgca acctattaca
     1741 gaatagagaa acgtaaaaaa gaaacactaa aaaatcctga gaaagctacc gaacgcatat
     1801 tggagcaaat caaaaaagat tttaggctaa aagaattacc aacacacatt gagtgttttg
     1861 ataactccaa tattcagggt agcaaccctg tatcggcatg tgttgttttc aaaaatgcca
     1921 agcccagtaa aaaagactac cgtattttca atgtaaaaac ggtggaaggt cctaacgatt
     1981 ttgcaactat ggaagaagcg gttacacgcc gttatcgtcg tttacttgat gaaaatcaac
     2041 ctttgcctca actcattgtt attgatggtg gaaaagggca actttctgct gcactcaaag
     2101 cattggacga agtgggattg cgtggaaaag tggcaatagt aggaattgca aaaaaattgg
     2161 aagaaatttt ctttccagga gattcgttac caatctacat cgataaacgt tctgaaagtt
     2221 taaaagtcat ccaacatttg cgtg
//
LOCUS       DAOQVQ010000420         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_60547_length_2238_cov_2.956024, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000420 DAOQVQ010000000
VERSION     DAOQVQ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60547_length_2238_cov_2.956024"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..2093)
                     /locus_tag="PKN22_10440"
     CDS             complement(141..2093)
                     /locus_tag="PKN22_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS43166.1"
                     /translation="MAKIKTSVAISQFADETASRAKYVVPALHALEAWNDYQPTKTHY
                     ATAQPTIRPLADNTTSPIESFLVWAGDAFRGGKDSKVAYDYIKANWSTNLFATQTAQS
                     TPDSYWNLMVHNSCYDLAVAEPTTPSFVGTLNLKSQLPKVNGKTEVVLFQNASIGTGI
                     QASNPWLQEMPDPMTKVTWDNCFTMNPADPAYTYRKTYDEQHGLSLATLTVGGNKITL
                     PVYPLPGQAIGTIGIALGYGRGADGEKIGKGAYQVAEFGGHELDENGKPKPIGANAFK
                     LVTFENGTYAYDTAGSLSGEEGEYLIAATQLHHTVMARQSIVRETTFEAYKHEDKEAY
                     NPAHKLQKINKEGHHEEVPISEFNLWEDHPVENVGHRWVMSIDLNQCFGCGSCLIACQ
                     AENNVPVVGKHEVRKGREMHWLRIDRYFSSDVEATIGTRKDPDTFDYHAAEVAEQNPK
                     VVHMPMMCQHCNHAPCETVCPVSATTHSNEGLNQMTYNRCIGTRYCANNCPYKVRRFN
                     WFNYPSYKKFTEINPAQDDLSRLVLNPDVTVRTRGVMEKCSFCVQNIQTGKLQAKVEG
                     RPVIDGEVKTACQEACSTDAISFGDWNDVDSLVRKQSSSKRSYQALEEVGTKPNIYYQ
                     VKVRNEKNDVLASIQHERVKEEEKHH"
BASE COUNT          607 a          461 c          411 g          759 t
ORIGIN      
        1 ttatcctcaa ttggacgaca aatgtcttcc gtaatttggt gataggtctt gtgacctaag
       61 atgaggggct ctcgaattgc tgcttcttta tgcattacaa ttggttttta ttcaattatt
      121 aattagttcc tttcttatta ttaatggtgt ttttcttctt ctttcactct ttcgtgttga
      181 attgaagcca atacatcatt tttttcatta cgtactttta cttggtagta aatgtttggt
      241 ttagtaccta cttcttctaa agcttggtat gaacgtttgc ttgaagattg tttacgtacc
      301 aatgagtcaa catcgttcca gtctccgaag ctaattgcat ctgtagaaca tgcttcttga
      361 catgctgttt taacctctcc gtcaataaca ggtcttcctt caactttagc ttgtaattta
      421 ccagtttgaa tattttgtac acagaatgaa catttctcca taaccccacg tgtacgtact
      481 gtaacatcag gattcaatac taaacgactt aaatcgtctt gtgcagggtt gatttcagta
      541 aatttcttgt acgatggata gttaaaccag ttaaaacgtc ttactttata aggacagttg
      601 ttagcacaat aacgtgtacc aatacatctg ttatacgtca tttggttcaa tccttcatta
      661 ctatgtgttg tagctgaaac cggacaaact gtttcacatg gtgcgtgatt acagtgttga
      721 cacatcatag gcatgtgaac aacttttgga ttttgttctg ccacttcagc tgcatggtaa
      781 tcaaacgtat ctggatcttt tcttgtacca attgtagctt ctacatcaga tgagaaataa
      841 cggtcaatac gcaaccagtg catttcacgt ccttttctta cttcgtgttt accaactaca
      901 ggtacgttgt tttcagcttg acaagctatt aaacaagaac cacaaccaaa acattggttc
      961 aaatcgatag acataaccca acggtgtcca acattttcaa ctgggtgatc ttcccataag
     1021 ttgaactcag aaattggaac ttcttcgtgg tgtccttctt tattgatttt ttgtaatttg
     1081 tgtgctggat tgtaagcttc tttatcttcg tgtttgtacg cttcaaaagt tgtttctcta
     1141 acaattgact gacgagccat tacagtgtgg tgtaattgtg ttgctgcaat caaatattct
     1201 ccttcttctc cgcttaatga tcccgcagta tcgtatgcat acgttccatt ttcaaaagtc
     1261 accaatttga atgcatttgc accaattggt tttggttttc cattttcgtc taactcatga
     1321 cctccaaatt ctgcaacttg ataagcccct ttaccgattt tttcaccatc agctccacga
     1381 ccgtatccta aagcgatacc aattgttccg attgcttgtc ctggtaatgg atatacaggt
     1441 aatgtaattt tatttccacc aacagttaat gttgctaaac ttaaaccgtg ttgctcatca
     1501 tacgttttac ggtatgtata agcagggtca gctgggttca ttgtgaagca gttatcccaa
     1561 gtaactttag tcattggatc tggcatctct tgcaaccaag ggttggatgc ttgtatacct
     1621 gtaccaattg aagcattttg gaataaaaca acttcagttt taccattaac ttttggcaac
     1681 tgtgatttta agttcaaagt accaacaaaa gatggagttg ttggctcagc aactgctaaa
     1741 tcgtagcaag aattgtgaac catcaaattc cagtatgaat ctggagtgct ttgagctgtt
     1801 tgagttgcaa ataaattagt tgaccaattt gctttgatat aatcgtaagc aactttagag
     1861 tctttaccac ctctgaatgc atcaccagcc caaactaaga acgattcgat tggtgatgtt
     1921 gtgttatcag ccaaaggacg aattgttggt tgtgctgttg cgtaatgtgt ttttgttggt
     1981 tggtaatcgt tccaagcttc caatgcatga agagcaggaa caacatattt agctctagaa
     2041 gcagtttcat cagcaaactg agaaattgct acagatgttt taattttagc cattgctttt
     2101 ccaaactcgc cttggccaag aaaacagcca ataaagccag ccacagcacc acgcccaaag
     2161 cattcgccaa ccaagcggcg gcacccgcag cgagcaataa gacagcgccg tacatcgggt
     2221 gtcgcaccca gcggtatg
//
LOCUS       DAOQVQ010000421         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_61639_length_2213_cov_2.664968, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000421 DAOQVQ010000000
VERSION     DAOQVQ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61639_length_2213_cov_2.664968"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PKN22_10445"
     CDS             complement(<1..823)
                     /locus_tag="PKN22_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43167.1"
                     /translation="MKQFIFVLTLITLSFSNVFAQCNNFIQADFDSYEYSTTVPHIIA
                     GTTYQNTPQGSTHGPNHTGNKHLYLNFVDGFTGVAYNRPYTVCVGGTFRLSFYHKEAW
                     GSNTNTTFNIYDGNNVLLYTQTVAWTGTTWNHFVSPELVSTTTTLRLEIVNNLTNSGS
                     NDMVVDDMLLEICSINENRTLSACDITGPVNLFSLFSAAMPTTGTWSGPATLGNGHLG
                     TYNTTLPSGTYTYTTAAAGTCGAPQGTVVVQDNVPVNLGADIHLCTPQAVTLNAGN"
     gene            1025..1570
                     /locus_tag="PKN22_10450"
     CDS             1025..1570
                     /locus_tag="PKN22_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43168.1"
                     /translation="MRTVLILIVAFLSLSVQFRNELRSVFGVRVSYQANGQMVTFIAY
                     YTDGVMETNRKFLTFNEFVHYASGEWPSIYNRNRMNLFELNGVQGGIFRDSITRKETP
                     YCFSVDSLWKLRFRNYPFKGSMEEGWSQDEFLPSPAQQKFLYKNYGMAHIDTKFFVDT
                     NFWKLLQDVTNEEWIAMYRNL"
     gene            complement(1660..>2213)
                     /locus_tag="PKN22_10455"
     CDS             complement(1660..>2213)
                     /locus_tag="PKN22_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNS43169.1"
                     /translation="VLDIKSSQKGARFVRFCDAFNIPLLVFEDVPGFLPGTDQEWNAI
                     ITNGAKLLYAFSEATVPRITVITRKAYGGAFDVMNSKSIGADLNFAWPTAEIAVMGAK
                     GAAEIIFKREIAEAADSNAKWLEKEAEYTEMFANPYRAAERGIVDDVILPEETRAKVI
                     AGFKMLENKAETLPQKKHGNIPL"
BASE COUNT          691 a          383 c          418 g          721 t
ORIGIN      
        1 tatttccagc attaagggtt acagcttgag gtgtacatag atgaatatca gcacctaaat
       61 tcacaggaac gttatcttga acaacaacag ttccttgagg tgcaccacag gttccagccg
      121 cagctgttgt atacgtgtat gttccactag gaagtgtagt attgtatgtg cctaaatgtc
      181 cattaccaag tgtagctggt ccgctccaag ttccagttgt tggcatggca gcactaaata
      241 aactgaataa atttactgga cctgtaatat cgcatgcaga taaagttcta ttttcattga
      301 tcgaacaaat ttcaagcaac atatcgtcca caaccatgtc gttactgccc gagttagtta
      361 gattattgac aatttctaag cgcaaagttg tggtagttga aaccaattct gggctcacaa
      421 aatgattcca tgtagttcct gtccatgcaa ctgtttgagt ataaagcaat acattattgc
      481 catcataaat attgaacgta gtgttggtat tacttcccca agcttctttg tggtagaaag
      541 ataaacgaaa agtaccacct acacaaaccg tataggggcg attgtaggca acacccgtaa
      601 atccgtccac aaaattgagg tataaatgtt tgttccccgt atgatttggg ccatgagtag
      661 agccttgagg cgtattttgg taagttgttc ctgcaataat atgaggaact gttgttgaat
      721 attcatacga atcaaaatcc gcttgaataa agttattgca ttgcgcaaaa acattagaaa
      781 atgatagggt tattaaggtt aaaacaaaga taaattgttt cataattgat taatgtattt
      841 ggtaccaaga cgtcattaaa acgttttagt tgcttttttg tgcaaattta gttacttttt
      901 taggattaat tatctagtaa taagaatatt tctatttttt caaagtattt aactgaaaca
      961 ctttgaaatg tttatcgtat aacaacaaac cacaacttgg tacgttttta gttgaagaaa
     1021 caatatgcga acagttttaa tactcatagt agcttttcta tcgttgtcgg ttcaatttcg
     1081 aaatgaattg cgctcagttt ttggtgtgcg tgtatcgtat caagcaaatg ggcaaatggt
     1141 gacttttatt gcgtattata ctgatggggt gatggaaacc aatcgtaaat ttttaacgtt
     1201 taatgagttt gtgcattatg cgtcgggaga gtggccgagc atttacaaca gaaatcgcat
     1261 gaatttgttt gaattgaatg gagttcaagg cggcattttt cgagattcca taacacgcaa
     1321 agaaacacct tattgttttt cagtagatag tttgtggaaa ttgcgtttta gaaattatcc
     1381 ttttaagggt agcatggagg aaggttggag tcaagatgaa tttttaccaa gtccggcaca
     1441 acagaaattt ttgtataaaa actacggcat ggcgcacata gatactaagt tttttgtaga
     1501 tactaatttt tggaaattat tgcaagatgt taccaacgaa gaatggatag caatgtatcg
     1561 aaatttataa tttcgttggg tgtaacccaa taaaaataaa gcaaaaaaaa tggaaccaat
     1621 taattcagtt ccatttttaa tttattgtta ccttttgatt tacaaaggaa tgtttccgtg
     1681 ttttttctga ggaagtgttt ctgctttgtt ttccaacatt ttgaaaccag caatcacttt
     1741 tgcacgtgtt tcttctggca aaatcacatc gtccacaatt cctctttcgg ctgcgcgata
     1801 tggattggca aacatttcag tatattcagc ttctttttcc aaccattttg cattggagtc
     1861 agcagcttca gcaatttcac gtttaaaaat aatttctgct gcacctttgg cacccattac
     1921 agcaatttca gcagttggcc aagcaaaatt caaatcggca ccgatagatt ttgagttcat
     1981 aacgtcaaat gcaccaccat aagctttgcg agtaatcact gtaattctag gaacggttgc
     2041 ctcagagaat gcatacaata attttgctcc attggtaata atggcattcc actcttgatc
     2101 agtacctggt aaaaatcctg gaacatcttc aaaaaccaat aatggaatgt taaatgcatc
     2161 gcaaaaacgc acaaatcgag ctcctttttg tgacgatttt atatctaaaa cac
//
LOCUS       DAOQVQ010000422         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_62697_length_2189_cov_3.476570, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000422 DAOQVQ010000000
VERSION     DAOQVQ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62697_length_2189_cov_3.476570"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PKN22_10460"
     CDS             complement(<1..975)
                     /locus_tag="PKN22_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS43170.1"
                     /translation="MGKETVVENTKKLDFETFKNEVLADYKLACISRETSLLGRKEVL
                     TGKAKFGIFGDGKELAQIALAKQFQDGDFRSGYYRDQTLMMAIGQLNVQQYFAGLYAH
                     TDEDVEPMTAGRQMGGHFGTRNLDKNGEWKDLMKQKNSSSDISPTAGQMPRLLGLALA
                     SKVYKNHPTLKDLEAFKKFTNGGNEVAFGSIGDASTSEGPFWETINAAGVLQVPMVMS
                     VWDDGYGISVTRKNQTTKDSISEALAGMQRTKDNPGYEIFVTKGWDYAHLCETYEKAV
                     AIARKEHVPVLVHVKEVNQPQGHSTSGSHERYKSEERLKWEVEFDCINK"
     gene            complement(1149..1556)
                     /locus_tag="PKN22_10465"
     CDS             complement(1149..1556)
                     /locus_tag="PKN22_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS43171.1"
                     /translation="MHIYLNSNMSLENLCNEYNNTDSSFYFIRINGNVAGYFKLNFSQ
                     KYTNNHEKKSLEIERLYLLKQVQNKGFGKQIMNTIFEVSKLKNISTIWLGVWENNEPA
                     IAFYKKMGFEEFGNHQFKLGLEIQIDKLLKINL"
     gene            complement(1683..>2189)
                     /locus_tag="PKN22_10470"
     CDS             complement(1683..>2189)
                     /locus_tag="PKN22_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNS43172.1"
                     /translation="EVYRGLNVASFSEDDFLFANQQVRILSGLYGILKPLDLLYPYRL
                     EMGTKWQISPKIKNLYAYWDDKISNYLTQELATDEAIINLASTEYFKAIQPKKIKNRI
                     ITPHFKDFKNGEYKVVMMYAKNARGKMAHYAVKNRIENPELLKEYNEDNYRFDSNLST
                     ENDWVFTR"
BASE COUNT          662 a          395 c          332 g          800 t
ORIGIN      
        1 tttattgata caatcgaatt ctacttccca tttcaaacgt tcttcagatt tgtatcgttc
       61 gtgcgaacca gatgttgagt gtccttgcgg ttgattcact tctttaacgt gtaccaaaac
      121 aggaacgtgt tcttttcggg caattgcaac tgctttttcg tatgtttcac acaaatgcgc
      181 ataatcccaa cctttggtta caaaaatttc ataacctggg ttgtcttttg tgcgttgcat
      241 tcctgccaaa gcttcagaaa tactgtcttt tgttgtttga tttttacgtg ttacagaaat
      301 tccataacca tcgtcccaaa cagacattac catcggaact tgcaacacac cagcggcatt
      361 gattgtttcc caaaacggcc cctcagaagt agaagcatca ccaatagaac caaaagcaac
      421 ttcgttacct ccgtttgtaa attttttaaa cgcttctaaa tcttttaatg tagggtgatt
      481 tttatacact tttgatgcca atgctaaacc taataagcgc ggcatttgtc cggcagttgg
      541 tgaaatatcc gacgaactat ttttttgttt catcaaatct ttccattcac catttttatc
      601 caagttgcgt gttccaaagt gcccacccat ttgtcgacca gcagtcatcg gttccacatc
      661 ttcatccgtg tgtgcgtata accctgcaaa atattgttga acatttagct gaccaatggc
      721 catcatcaat gtttgatcgc ggtaataacc agagcgaaaa tcaccatctt gaaattgttt
      781 agccaatgca atttgtgcca attcttttcc atcgccaaaa attccgaatt ttgctttacc
      841 agtcaacact tcttttctac ccaaaaggga agtttctcta gaaatgcaag ctaacttata
      901 atcagctaaa acttcgtttt taaatgtttc aaaatcaagt tttttagtgt tttctacaac
      961 tgtttctttg cccatagctt tattttaaga tgttacaaat atagcaaaat tcccccctta
     1021 aaatttgaat gcaaacgatt aattagagaa aatacacatt tttaaacaga tttcatagta
     1081 aaaagaacgt taattggata ttttgaattt taaaaaagtg atggctgtaa aaagaattta
     1141 ataaaatatt agagattgat ttttaaaagt ttatctattt gaatctcaag tccaagttta
     1201 aattgatgat ttccaaattc ctcaaatccc atttttttat aaaaagcaat tgcaggctcg
     1261 ttattttccc acacacctaa ccatatagtt gaaatatttt tcaattttga gacttcaaaa
     1321 atggtgttca ttatttgttt tccaaatcct ttattttgaa cttgttttaa taaatacaat
     1381 cgttcaattt caagtgattt tttttcatga ttgttggtgt atttttgaga aaaatttagc
     1441 ttaaaataac ctgctacatt tccgtttatt cgaatgaagt aaaaactaga atcagtatta
     1501 ttgtactcat tgcatagatt ttccaaagac atgttactat tcaggtatat atgcatgttt
     1561 tcgggggtgt tttgattgga aaaagtttca aaaaaagtta aacgactaat gcgttgcagc
     1621 aattctagtt cgctggaagt tactttatcg atttgaagtt gcatattttc catattttcc
     1681 ctttagcgtg taaataccca atcattttcg gttgataaat tactatcaaa acgataattg
     1741 tcttcgttgt attcttttag taattcaggg ttctcaattc tgttttttac agcgtaatgt
     1801 gccatttttc cacgtgcgtt tttagcatac atcattacaa ctttgtattc accattttta
     1861 aaatctttaa aatgaggagt aatgatgcga ttttttatct ttttaggttg aatcgcctta
     1921 aaatattctg ttgatgcaag gttgataatt gcttcatcag ttgctaattc ttgcgttaag
     1981 taattggaaa ttttatcgtc ccaataagca taaagatttt taatttttgg tgaaatttgc
     2041 cattttgttc ccatttctaa tcggtaggga tatagcaaat ctaaaggttt taaaatacca
     2101 tacaagcctg ataaaatgcg cacttgttga ttggcaaaaa gaaaatcatc ttcagaaaat
     2161 gacgcaacgt ttaatcctcg gtaaacttc
//
LOCUS       DAOQVQ010000423         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_62739_length_2188_cov_4.076418, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000423 DAOQVQ010000000
VERSION     DAOQVQ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62739_length_2188_cov_4.076418"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..540
                     /locus_tag="PKN22_10475"
     CDS             160..540
                     /locus_tag="PKN22_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNS43173.1"
                     /translation="MSKLNELKKSILADGVIDEQEVKQLREVLYADGVIDKEEAEFLF
                     ELNDAVSGKENHPSWETLFVDAITSFLLEDETSPGVVDEEEAKWLLAKIQGDGKLDKI
                     ELTLLNNLKAKSKQLPQSLLNLLK"
     gene            542..>2188
                     /locus_tag="PKN22_10480"
     CDS             542..>2188
                     /locus_tag="PKN22_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS43174.1"
                     /translation="MEHKKHYTGLSNEQVLESRNKFGVNILTPPEREPLWKLFLEKFE
                     DPIIRILLIAAFLSLGISFVHMEFAETIGIFCAILLATGVAFWFEMDANKKFDVLNKV
                     NDDTLYKVIRNGNICEIPKKEIVVGDIVILGTGEEVPADGELLEAVSLQIDESCLTGE
                     PIIDKTNNPANFHSDSTYPSNWAMRGTKLMDGHGILEIKKVGDETEFGKVAEKATEMS
                     GEETPLNKQLDKLAKFIGVVGFALAVLTFLALFVKDLFLGEAQYTIGQLGSIGIVILS
                     ASIVLTKVWLPIIYDSFELLGKEKELPKSVEEGSWFRWILYGIGIFVLLASAGYLFGI
                     NPLNSESWVSLDVAGRILQYFMVAVTLIVVAVPEGLPMSVTLSLALSMRRMLQNNNLV
                     RKMHACETMGATTVICTDKTGTLTQNQMQVYKADFFCLNGNKLLNEENHNIIKESISV
                     NSTAYLDYSEPTKIKTLGNPTEAALLLWLHEQGINYLDIREDAEVIEQLTFSTERKFM
                     ASLVKSPLLNKKVLYVKGAPEIVLSKCSYILSEQGLKPINE"
BASE COUNT          774 a          303 c          429 g          682 t
ORIGIN      
        1 taagaaaatt gaaattggcg aagctgttga attaactgaa ggcaaaaaaa ttcttttatc
       61 caaagaagat ggaggtagat taattatagt acaattagta aaatcataaa ccgtaatatt
      121 tcaaaatcct tttattgtca aacttaaaaa ttttttaaaa tgagcaaatt aaatgaacta
      181 aaaaaatcaa ttctcgccga tggagtaatt gatgaacaag aagtaaaaca attacgtgaa
      241 gtattatatg ctgatggcgt aattgacaag gaagaagcag aatttctgtt cgagttaaac
      301 gatgctgtaa gtggaaaaga aaatcatcca agttgggaaa cgctttttgt tgatgcaata
      361 accagtttct tattagaaga tgaaacatct ccaggtgttg ttgatgaaga agaagcaaaa
      421 tggcttttag caaaaattca aggtgatgga aagttggata aaattgaact taccttatta
      481 aataacctaa aagcaaaatc gaagcaatta cctcaatcat tattaaattt attgaaataa
      541 gatggaacac aagaagcact acaccggact ttctaatgag caagtcctcg aaagccgtaa
      601 taaatttgga gtaaatattt taactccacc agagagagaa cctttatgga agcttttttt
      661 agaaaagttc gaagacccaa ttatcagaat acttttaatt gcggcatttc tttcattagg
      721 tatttcattt gttcacatgg agtttgctga aacaataggt atcttttgcg ctattctttt
      781 agcaaccggt gttgcatttt ggtttgagat ggacgcaaat aaaaaatttg atgttttaaa
      841 caaagttaat gacgacacgc tttacaaggt aatacgaaat ggaaatattt gcgaaatacc
      901 aaaaaaagaa atcgttgttg gtgatattgt aattcttggt acaggcgaag aagttcctgc
      961 tgatggtgaa ttactcgaag cagtgtctct tcaaattgat gaatcctgtt taactggtga
     1021 gccgataatt gataaaacta acaacccagc taattttcat tcggattcga catatccttc
     1081 gaattgggca atgagaggaa caaaattgat ggatggtcac ggtattctag agataaagaa
     1141 agttggcgac gaaacagaat ttggtaaagt tgcagagaaa gcaacagaaa tgagtggtga
     1201 agaaacacca ttgaataagc aattggacaa actggctaaa tttattggtg ttgttggttt
     1261 tgccttagct gtattaacat ttctcgcact ttttgttaaa gatttattct taggggaagc
     1321 tcaatataca attggtcaat taggttcaat tggcattgta attttatctg cttcaatagt
     1381 cttgaccaaa gtttggttgc caatcatcta cgattcattt gaattgttag gtaaagaaaa
     1441 ggaattacct aaaagtgttg aagaaggtag ttggtttaga tggatattat atggtattgg
     1501 aatatttgtg ctacttgcct ctgctggtta tttatttggt ataaaccctt taaattctga
     1561 atcttgggtt agcttagatg ttgcaggtcg gatactgcaa tattttatgg tagcggttac
     1621 attaatcgta gttgcagttc cggaaggttt gccaatgagt gtaaccctaa gtttagcatt
     1681 aagcatgcgt agaatgcttc aaaacaataa tcttgtgaga aagatgcacg cttgtgaaac
     1741 aatgggggca acaacggtta tttgtactga caaaactggc actttgaccc aaaatcaaat
     1801 gcaagtttat aaagccgatt ttttctgttt aaatgggaat aaacttttaa atgaagaaaa
     1861 ccataatatt atcaaagaga gtatttctgt aaattcaaca gcatatcttg attattctga
     1921 acccacaaaa ataaaaacgt taggcaaccc aacagaagct gctcttttat tgtggttaca
     1981 tgagcaaggc ataaattatt tagatattcg tgaagatgct gaagtaatcg aacaactaac
     2041 attttcaacg gaaagaaaat ttatggcttc attggttaaa agtcctttgc taaacaaaaa
     2101 agttctatat gtgaagggtg cacctgaaat tgtgctttca aaatgtagtt atatattgtc
     2161 tgaacaaggg ttgaaaccaa ttaatgaa
//
LOCUS       DAOQVQ010000424         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_62954_length_2183_cov_4.631579, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000424 DAOQVQ010000000
VERSION     DAOQVQ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62954_length_2183_cov_4.631579"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PKN22_10485"
     CDS             <1..1169
                     /locus_tag="PKN22_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNS43175.1"
                     /translation="SLMALLEDKKVTINDEVNATGEYRFFNNVMKDHNGGFGKITIKQ
                     AFEHSSNVFTKIVYNAYKSNPMQYINRLRSFGLADSLGLDIKGEAKPTMYYPKHPNWS
                     GLSLPWMAVGYEVQQTPMQTLAFYNAVANNGRFMKPQFVKEVRRGQEVIKTFAPVALH
                     ERICSQNTINQLKECLEGVMLEGTGKRLKSSFFTSAGKTGTARVLNADNSYGDKGSYK
                     YQASFVGYFPADEPMYSCIVVVSEPESDFYGAIVSGTVFASIANKVYASALKYHDAIN
                     EKKQRLQEAPVSKGGNKNDLEKVLNAFKIRFDLEGDGEWLSTSKQDNFVRLTPQKTID
                     KLVPNVVGMNAKDAVFLIEKAGMKVKLNGYGTVSTQSIAAGTRVSEQTIVLTLK"
     gene            1171..>2183
                     /locus_tag="PKN22_10490"
     CDS             1171..>2183
                     /locus_tag="PKN22_10490"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNS43176.1"
                     /translation="MEKIVKDLLYGVQLTTVVGSTDATVTNLVLDSREAKDGSLFFAI
                     GGVKFDGHDFIPAVIAQGCKVIIAEKEVAVPADVTLIVTNNSHKAAGIIASNFYGEPS
                     TQLRLIGTTGTNGKTTTSTLLYNLFTKLGYQVGLISTVVNKIGDQEIPSTHTTPNPVA
                     LNELLAKMVDEGCAYCFMEVSSHAIHQNRIAGLTFAGGVFTNITHDHLDYHGTFKEYL
                     DVKKAFFDQLPKTAFALTNIDDKNGRYIVQNTPAKVITYALKTPADCKVKVIENQFSG
                     LELNINGKEIWSRLVGDFNAYNILAAYSVAMQLHEDETDVLRVLSSLESVEGRFQYFV
                     AANG"
BASE COUNT          730 a          392 c          454 g          607 t
ORIGIN      
        1 cttcattaat ggctttgttg gaagacaaaa aagtgacaat aaacgatgaa gtaaatgcca
       61 ctggtgaata ccgctttttc aacaatgtga tgaaagacca caacggtggg tttggcaaaa
      121 taaccattaa acaagctttt gaacactcct ccaacgtgtt tacaaaaatt gtttacaatg
      181 catacaaaag caatcctatg caatacatca atcgtttacg cagttttgga ttggcggatt
      241 cattgggatt ggacatcaaa ggtgaagcaa aaccaacgat gtattatcca aaacacccaa
      301 attggtcggg attgtcattg ccatggatgg cggttggtta tgaagtgcag caaactccta
      361 tgcaaactct agctttttac aatgctgttg ccaataacgg gcgttttatg aaacctcaat
      421 ttgtgaaaga agtaagacgt ggacaagaag ttatcaaaac ctttgcccct gttgcattgc
      481 acgaacgaat ttgctctcaa aacaccatca accaactaaa agaatgtttg gaaggtgtta
      541 tgttggaagg aacaggaaaa cgcttaaaat cgtcgttttt tactagtgcc ggaaaaacag
      601 gtacagcccg cgttttgaac gccgataatt cttatggcga taaaggaagt tacaaatacc
      661 aagcttcgtt tgttggctat ttcccagcag atgaaccgat gtattcgtgc attgttgtgg
      721 tatcggagcc agaatctgat ttttatggag ccatcgtttc ggggactgta tttgcatcca
      781 ttgccaacaa agtatatgcc tctgcattaa aataccacga tgcgattaat gaaaagaaac
      841 aacgtttgca agaagctcca gtttcgaaag gtggaaataa aaacgacttg gaaaaagtgt
      901 taaacgcttt taaaattcgc tttgatttag aaggagatgg cgaatggttg agcacttcaa
      961 aacaagataa tttcgttcgt ttaacacctc aaaaaacaat cgataaattg gtgccaaatg
     1021 tagtgggaat gaacgctaaa gatgctgtat tccttattga aaaagcagga atgaaagtaa
     1081 aactaaatgg ctatggaaca gtgagtacac aatccattgc tgcgggaaca agagtaagtg
     1141 aacaaaccat tgttttaaca ttaaaataaa atggaaaaaa tagttaaaga cctattgtat
     1201 ggcgttcaat taacgacagt tgttggttca accgacgcaa ctgttaccaa cttagtattg
     1261 gactctcgcg aagctaaaga tggcagtctt ttctttgcaa taggcggcgt aaaatttgat
     1321 ggacatgatt tcatcccagc agtgattgca caaggttgta aagtgataat tgctgaaaaa
     1381 gaagttgctg ttcctgctga tgtaacgtta attgttacca acaactcaca caaagcagct
     1441 ggtattattg caagtaattt ttatggagag ccttcaacgc aattgcgctt aattggaaca
     1501 acaggtacca acggaaaaac aaccacttct acactgttgt acaatttatt tacaaaattg
     1561 ggttaccaag taggattaat ttctacggtt gttaacaaaa ttggcgatca ggaaatacca
     1621 tctacacata ccacacccaa cccagttgca ctcaacgaat tgttggcaaa aatggtggat
     1681 gaaggttgtg cctattgttt tatggaagtg agttcgcatg ctattcatca aaaccgtatt
     1741 gctgggttaa catttgccgg aggcgtattt accaacatta cacacgatca tttggattat
     1801 cacggaacgt ttaaagaata cttagacgtt aaaaaagcat tctttgatca attgcccaaa
     1861 acagcgtttg cattgaccaa tattgacgat aaaaacggtc gctacatagt gcaaaacacc
     1921 ccagcaaaag taattacgta tgctcttaaa acaccagccg attgcaaagt aaaagtaatt
     1981 gaaaatcaat tttctgggtt ggaattaaac atcaacggga aagaaatttg gtcgcgtttg
     2041 gttggagatt tcaatgccta caatattttg gcagcatact cagtagccat gcaattacac
     2101 gaagatgaaa ccgatgtgtt gcgtgttttg agttcattgg aatcggttga agggcgtttt
     2161 caatattttg tggcagcaaa cgg
//
LOCUS       DAOQVQ010000425         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_63497_length_2172_cov_4.297119, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000425 DAOQVQ010000000
VERSION     DAOQVQ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63497_length_2172_cov_4.297119"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PKN22_10495"
     CDS             complement(<1..1041)
                     /locus_tag="PKN22_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNS43177.1"
                     /translation="MNAKHRNISVAGRSYFFNALFVLLNLAGLTMVTIAYHDSQVNNA
                     IGLKIAGFALMAVTIAGLLIFRGRLMMANVSRALIGGLFIVSGLVKANDPLGFAYKLE
                     EYFEDGALAYRIKEWFGIPGFSLEFLIDYALPISVIICIVEIIIGILLIVGEKIKPVS
                     YILVLMMGFFTFLTWHTATCDSSKKFKDRDTYEISNPIVASKIEEAKTNKDIKIVSHT
                     GQEVVIDEMKQPQCVDDCGCFGDAMKGSIGRSLTPKESLWKDIIVFYLGFWIFLAQWI
                     IVPNNRKQNIVFGLFSLLVVVFFSMIFSWYFPVLFGFIGIAGALWVKNKGGVLLGNAW
                     GMSLFITLISGVF"
     gene            complement(1053..1598)
                     /locus_tag="PKN22_10500"
     CDS             complement(1053..1598)
                     /locus_tag="PKN22_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNS43178.1"
                     /translation="MSQTDAQYTKVIANCKEIYSKKTKDYGTSWRILRPSSLTDQLFI
                     KAQRIRTIQETGVSKVNESIDGEFIAIVNYCIMALIQLNEKELKELELSEDEAISLYE
                     KYAEQAYQLMKKKNHDYGEAWRDMRVSSLTDLILTKILRVKQIEDNKGNTIISEGIDA
                     NYFDMLNYAVFALIHLTQETN"
     gene            1610..>2172
                     /locus_tag="PKN22_10505"
     CDS             1610..>2172
                     /locus_tag="PKN22_10505"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNS43179.1"
                     /translation="MQNSSVEDTNYKANSIYINGKLTDFTIPKIMGILNVTENSFYAD
                     SRLKSDSELLNMVEKHLTEGADYLDVGGFSTRPGAELVEETIEQKRIESALLTIKKSF
                     PEAILSVDTFRGSVAQIAIDGGAAIINDISGFQFDASLLEVISKSQVAYVLMHIASSF
                     EKMHEVTQNRNIDISTYFDINKKVNDY"
BASE COUNT          704 a          393 c          374 g          701 t
ORIGIN      
        1 aaaaacacct gaaataagag tgataaaaag gctcattccc catgcattgc ctaataaaac
       61 tccgccttta tttttaaccc ataatgcacc agcaatacca ataaatccaa ataatactgg
      121 gaaataccac gagaaaatca tggagaaaaa taccaccact aatagagaaa acaatccaaa
      181 tacgatgttt tgtttgcgat tattgggaac gataatccat tgtgctaaga atatccaaaa
      241 tcctaagtaa aaaacaatta tatctttcca gagtgattct tttggtgtta aagagcgacc
      301 aattgagcct ttcatggcat cgccaaaaca tccacaatcg tcaacacatt gcggttgttt
      361 catttcatca atcactactt cttgccctgt atggcttaca attttaatat ccttgtttgt
      421 tttagcttct tcaattttag aggctacaat ggggttagaa atttcatagg tatctctgtc
      481 tttaaatttt ttgctcgaat cacacgttgc tgtatgccaa gttaaaaatg taaagaatcc
      541 catcatgagt actaaaatgt aggatactgg ttttattttt tcacctacaa ttaataaaat
      601 accgatgatg atttcaacga tgcaaataat aacactgatg ggcagcgcat aatcaattaa
      661 aaactcgagt gaaaaccctg gtattccaaa ccattctttg atgcggtaag ccaatgctcc
      721 gtcttcaaaa tattcttcaa gcttgtaggc aaaacctaat ggatcgttgg cttttaccaa
      781 tccggagacg atgaaaagtc caccgattaa tgcacgtgaa acattggcca tcatcaatct
      841 tcccctaaaa ataaggagac cagcaatggt tacggccatt aatgcaaaac ctgcaatttt
      901 taagcctata gcattattta cttggctatc gtggtaagca atggttacca tagttaaccc
      961 tgctaagttt aatagtacaa aaagagcgtt aaaaaaataa gaccttccgg caacagaaat
     1021 gtttctatgt ttagcgttca tttgattata gtttaattag tttcttgtgt taaatgaatg
     1081 agtgcaaaaa ctgcataatt gagcatgtca aaataattgg catcaatacc ttcactgatg
     1141 atggtgtttc ctttgttgtc ttcaatttgt ttgactctta gaattttggt taaaatcaaa
     1201 tcggtaagcg aactcacacg catgtcgcgc caagcttcac catagtcgtg attttttttc
     1261 ttcatcaatt gataagcttg ctctgcatat ttttcataca acgaaatagc ctcatcttcc
     1321 gataattcca gctctttcaa ctctttttca ttgagttgaa tcaacgccat aatgcaatag
     1381 ttaactatgg caataaattc accgtcgatg gattcgttga ctttacttac gcctgtttct
     1441 tgaatcgtgc gaatgcgttg tgctttgata aataattgat cggttaatga acttggtcga
     1501 agtattctcc aagaggtacc gtaatctttt gtttttttgc tgtaaatctc cttacagtta
     1561 gctattactt ttgtatattg cgcgtcagtt tggctcatta ttttataaaa tgcaaaattc
     1621 aagcgttgaa gatacaaatt ataaagcgaa ttccatttat attaatggaa aattaacgga
     1681 ttttaccatt ccaaaaatta tggggatact caatgtaacc gaaaattcgt tttatgctga
     1741 tagtcgctta aagtctgatt ctgaattgct taatatggtt gagaaacatt tgaccgaagg
     1801 agccgattat ttagatgttg gaggcttttc tactcgccca ggagccgaat tagtggaaga
     1861 aacaattgag caaaaacgta ttgaatcagc acttttaacc attaagaaaa gttttccaga
     1921 agcaattcta tcagtagata cttttagagg aagcgttgct caaattgcta tcgatggagg
     1981 agcagccatt atcaatgata ttagtggttt tcaatttgat gcaagtttgt tggaggtgat
     2041 ttcaaaaagc caagttgcct atgttttgat gcacattgca agtagttttg aaaagatgca
     2101 cgaggtgaca caaaatcgca atatcgacat atcgacatat ttcgatatca acaaaaaagt
     2161 gaatgattat tt
//
LOCUS       DAOQVQ010000426         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_63573_length_2171_cov_2.517013, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000426 DAOQVQ010000000
VERSION     DAOQVQ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63573_length_2171_cov_2.517013"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKN22_10510"
     CDS             <1..373
                     /locus_tag="PKN22_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNS43180.1"
                     /translation="LVNYLSKNEKQNKSYVTTEKDKNAKKAILSYQTLSSSDKYHLLE
                     VNLETGRHHQIRCQLANIGCIIKGDLKYGANRSNPDGSICLHARSLSFTHPTTKEEIK
                     LTAPVPKDTLWMFFEEAVNNK"
     gene            complement(387..1052)
                     /locus_tag="PKN22_10515"
     CDS             complement(387..1052)
                     /locus_tag="PKN22_10515"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNS43181.1"
                     /translation="MQQQFKGLKEFNEGMAAFTGLDGRSGFINTKGEIIIQPIYKNVG
                     HFSNGLAWVRDFSGKVGFIDKKGKLVIQSKYDVVTPFDPIAKRATARIGKQTLILKED
                     GTEIITSVNITIKKFVGGVALASEAGRWGYLDKDGKWLVSPKYENAYDFSYGIGVVEL
                     GGFWGIVGADGKILLEPTYSKITEFKNGYANVKRGDKWGIINKEGKEVLAPKFIGLKE
                     FRP"
     gene            complement(1086..1535)
                     /locus_tag="PKN22_10520"
     CDS             complement(1086..1535)
                     /locus_tag="PKN22_10520"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNS43182.1"
                     /translation="MLTRKLIIASIFTVLTFGIFAQNYFGPFSNDNKLWGFTDYQGKE
                     MVSPKYVRPTYVTQKGVAGVYVPAEKRFKLINMKDEEITNSLPGVVTSFKYIIDEESK
                     EVAEYFYFAVNKKWGIMDASGKVLFNPNYDHLSKFSGGCAVAKIGSA"
     gene            1656..>2171
                     /locus_tag="PKN22_10525"
     CDS             1656..>2171
                     /locus_tag="PKN22_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43183.1"
                     /translation="MKQFLFILLFILPLSVGFSQDNTVWLRPNMGQWHENICYQVNLN
                     SGKMYVEADGFTYHFYDAPASNHQHHNHTTHDDETVKNHVVKSKFLGSSWSRQALEAE
                     KSSFYENYYLGNNTSSWKSEVYNYKKVTLQNFYPSIDLHLDGSQEKLKYSFIVAPHQA
                     ANQIKWQVEGAN"
BASE COUNT          705 a          425 c          317 g          724 t
ORIGIN      
        1 attggtaaac tatttatcaa aaaacgaaaa acaaaacaaa tcctacgtca ctacagaaaa
       61 ggataaaaat gccaaaaaag caattttatc gtatcaaact ttaagttctt ctgacaagta
      121 ccatctttta gaagtgaatt tagaaacggg gcgtcaccac caaattcgtt gtcagttggc
      181 aaacataggt tgtatcatca aaggcgactt aaaatatggt gccaatcgta gcaatcctga
      241 tgggtcgatt tgcttgcatg cacgttcatt atcgtttaca catccaacta caaaagaaga
      301 aataaaatta acggctcctg taccaaaaga caccttgtgg atgttttttg aagaagccgt
      361 taataataaa taaatcgttg cttttcttaa ggtctaaatt cctttaaacc aataaattta
      421 ggtgctaaaa cttctttccc ttctttatta atgattcccc atttatcgcc acgttttaca
      481 ttggcataac cattcttgaa ttctgtaatt ttagaatacg ttggttctaa tagtattttg
      541 ccatcagcac caacaattcc ccaaaaacca ccaagctcaa cgacacctat tccgtatgag
      601 aaatcatatg cattttcata cttagggcta accaaccatt ttccatcttt atccaaatag
      661 ccccatcttc ctgcttcgct agcaagcgcc acaccaccaa caaatttttt aatggtaatg
      721 ttgacagaag ttataatttc agttccgtct tcttttaaaa tcaatgtttg tttaccgatt
      781 cttgctgttg cgcgttttgc tataggatca aatggtgtta ccacatcgta ttttgactga
      841 ataaccaact tacctttttt gtcgataaag cccactttac cgctaaaatc cctaacccaa
      901 gcaagtccat ttgagaaatg accaacattt ttataaatag gctgaattat aatttctcct
      961 ttagtattga taaacccact tcttccatca agtccagtaa aagcagccat cccttcgtta
     1021 aattctttta atcctttgaa ttgttgttgc aactgatata cttttccatc aggatacaaa
     1081 ataaatcacg cacttccaat ttttgcaacg gcacaacctc cagaaaattt agaaagatgg
     1141 tcgtagttag ggttaaacaa cactttacca cttgcatcca ttatccccca ttttttgttg
     1201 acagcaaaat aaaaatactc tgctacttcc ttactttctt catctattat atacttaaat
     1261 gatgtaacta cacctggtaa tgaattggta atttcctcat ctttcatgtt gatgagtttg
     1321 aatcgttttt ctgctggaac atacacgcca gcaactcctt tttgggttac atacgttgga
     1381 cgaacatatt ttgggctaac catttcttta ccctgataat cggtaaagcc ccataattta
     1441 ttgtcatttg aaaagggccc aaaataattt tgtgcaaaaa ttccaaacgt caaaacagta
     1501 aaaatacttg cgataattaa ttttcttgtc aacataatat tcaatttagt ggttttatat
     1561 acgttttcta ggcaactaaa atacgttttt tttgtcatta tcaatgagat aatgtgtatt
     1621 tttatatttt aaaaaaatca attttatcct gaataatgaa acagtttctt ttcattttat
     1681 tgtttattct tccattatcg gttgggtttt ctcaggataa tacggtatgg ttgcgtccaa
     1741 acatgggaca atggcatgaa aacatttgtt accaagttaa tttgaatagc ggtaaaatgt
     1801 atgttgaggc agatggcttt acctatcatt tttacgatgc tcctgcatct aatcaccaac
     1861 accataatca tacaactcac gacgatgaaa cagttaaaaa ccacgtggtt aaaagtaaat
     1921 ttttgggtag tagttggtcc cgtcaagcat tggaagctga aaaaagttcg ttttacgaaa
     1981 attattatct tggaaacaat acatctagtt ggaaatcgga agtttacaac tataaaaaag
     2041 ttacacttca aaacttttat ccttccattg atttgcatct agatggcagt caagaaaaac
     2101 taaaatattc ttttattgtt gctcctcatc aagcagcaaa ccagattaaa tggcaagtgg
     2161 aaggtgctaa c
//
LOCUS       DAOQVQ010000427         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_63901_length_2164_cov_3.347558, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000427 DAOQVQ010000000
VERSION     DAOQVQ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63901_length_2164_cov_3.347558"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(530..1825)
                     /gene="porV"
                     /locus_tag="PKN22_10530"
     CDS             complement(530..1825)
                     /gene="porV"
                     /locus_tag="PKN22_10530"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNS43184.1"
                     /translation="MKKSFLFLLATTFTSIGFNALAQPGATISDDDKSKLELNTITTA
                     LPFMSITPDSRAGGMGDAGTALSGSSTSLYWNTSMLIFAPDKSEISISYTPWLRNLTN
                     DMHLSYAAGYYKFGRHAVGLGLRFFSLGEITYTDNNANPIRTDKPSEFQLTGGYAFKL
                     SNRLSIGLNAHFAYSNLTGGYVVGNNGAKPAMAGAADLSLTYLKDDAKIGNMDGEYTF
                     AFTLNNIGNKVSYSYSQQRDFIPMNLKIANAYKMKFDRYNSLFIGLELQKLLVPTPPI
                     YGLDANGDRVIFAGKSSDVGVIKGLVQSFYDAPGTIVKDEDGNYVQNEDGSYQIKKGS
                     RFVEELSEINVALGLEYWYNNLLAIRAGYFFEAPNKGKRQFFSFGAGVKYNRFGLDIS
                     YLAAVGGRRSPLANTVRFTLRFTLGKINNGKNADEDKPE"
     gene            complement(1886..>2164)
                     /locus_tag="PKN22_10535"
     CDS             complement(1886..>2164)
                     /locus_tag="PKN22_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43185.1"
                     /translation="PNPFTTSTEFFFEHNQCCTELETQIQIFTISGKLVKTINQTTYT
                     QGYRSNGIHWNGLDDFGDKLARGVYVYRLKVRTPLGETAEKLEKLVIL"
BASE COUNT          703 a          395 c          352 g          714 t
ORIGIN      
        1 caacataaaa ttaaatgtat cttctctaaa gagaggaaca tatattttga aaattaaagc
       61 aaatgaggca cagtcaaaaa cacaagtaat tgttttagag taatcattaa atacaaacat
      121 tcttcaaccg actttcagaa atggaagtcg gtttttttat tattttaatt ctacatacat
      181 tcccatattg taattgtcgt tttgtttttc atcaacttta aaacttaaga taataactaa
      241 tgttattttt tcgaacagaa agatgatttt tcataactcg ttgtgcattt gctgatatat
      301 ggaaataaaa aaataacagc ataaatactc aaaaaaaccg ctaaagttta tgagaaatct
      361 ttttttcaat acaattgctt ttaactaaat gcacaatggg ttttctaaaa taaatagata
      421 cacctggttt ctttttcaag acacaattta atcctaaaat aaacgatttt ttgaagtaat
      481 aaaaaaagcc cacgattgct cgtaggctgc tgtattttaa aatccactat tattctggtt
      541 tatcttcgtc ggcgttttta ccattattga ttttacccaa tgtaaaacga agtgtgaaac
      601 gaacagtgtt tgccaatgga gaacgtcttc cgccaacagc tgctaagtag gagatatcca
      661 atccaaaacg gttgtatttt acacctgcac caaagctaaa aaattgacgt tttcctttgt
      721 ttggagcttc aaagaaataa cctgcacgga tggctaataa attattatac cagtattcca
      781 atccaagtgc tacgttgatt tcagaaagtt cttcaacaaa acgacttcct tttttgattt
      841 ggtaggaacc atcttcgttt tgcacataat taccatcttc gtctttaacg atcgttcctg
      901 gcgcatcgta aaacgattga accaatcctt taataactcc cacatcagat gattttccag
      961 caaaaattac acggtcacca ttagcatcca aaccataaat tggtggtgtt ggaactaata
     1021 atttttgtaa ttctaagcca ataaacaacg agttgtaacg gtcaaatttc attttatagg
     1081 cattggcaat tttcaagttc atcggaatga aatcacgttg ttgtgaataa gaataactta
     1141 ctttgttacc aatgttatta agggtaaaag caaatgtgta ttcaccgtcc atgttgccta
     1201 ttttagcatc gtcttttaaa tacgtcaacg ataaatctgc agctcctgcc attgctggtt
     1261 tagcaccatt gttacccact acataaccac ctgttaagtt ggaataagca aaatgagcat
     1321 tcaaaccaat acttaaacgg ttgcttaatt taaacgcata tccacccgta agttgaaact
     1381 cacttggttt gtctgttctg attggattgg cattattgtc ggtataagta atctcaccca
     1441 agctaaagaa acgcaaacct aaaccaacag catgacgacc aaatttgtag taacctgctg
     1501 catacgataa gtgcatgtcg ttagtcagat tacgtaacca tggtgtatat gaaatgctaa
     1561 tttcggattt gtctggagca aaaatcaaca tagaagtatt ccaatacaac gatgtagatg
     1621 aaccactcaa agctgttcct gcgtcgccca tacctcctgc acgagaatcg ggtgtaatag
     1681 acataaaagg caatgccgta gtgatggtat ttaattccaa cttgcttttg tcgtcatcag
     1741 aaatagtagc tccaggctgt gctaatgcgt taaatcctat gcttgtaaat gttgttgcta
     1801 ataaaaataa aaaacttttt ttcatgctgt tttattaatg cggatggcaa atatacgttt
     1861 ttatacttcc tttattgtgt tgaacttata atattactaa tttttctaat ttttctgctg
     1921 tttcgccaag tggagttctc acttttaagc ggtaaacata cacgcctctt gctagtttgt
     1981 cgccaaaatc atccaatcca ttccagtgaa taccgttaga acgataacct tgcgtatagg
     2041 tggtttgatt gatggttttt accaatttac cagaaatagt aaatatctga atttgcgttt
     2101 ctaactcggt gcaacattgg ttgtgctcaa agaaaaattc ggtgctggta gtaaacggat
     2161 tagg
//
LOCUS       DAOQVQ010000428         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_64354_length_2154_cov_3.800858, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000428 DAOQVQ010000000
VERSION     DAOQVQ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64354_length_2154_cov_3.800858"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKN22_10540"
     CDS             complement(<1..1040)
                     /locus_tag="PKN22_10540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNS43186.1"
                     /translation="MSEDLEKSKIKNEGVLERTFSTETMDGQIATLNLGPTHPATHGI
                     FQNILKVDGEKILSAEPTVGYIHRAFEKMAERKSFQQITTLTDRLNYCSSPLNNIGWQ
                     MTVEKLCGIEIPKRVQYMRVIIMELARIADHLICNSVIGVDTGGLTGFTWMFIERERI
                     YDMYEQICGTRLTTNIGRIGGFERDLNDKFHALIQDFLNTFLKRFEEYNALLDRNRIF
                     MDRTIGVGKISAERALSYGFVGPNLRATGIDYDVRVHSPYCSYEDFDFDIPVGTEGDT
                     YDRFQVRQEEVRQSIRIINQAYENLPKEGVYHADTPLFYLPPKENVYNTIEGMINHFK
                     QVYGEMPYPVGEV"
     gene            complement(1052..1567)
                     /locus_tag="PKN22_10545"
     CDS             complement(1052..1567)
                     /locus_tag="PKN22_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNS43187.1"
                     /translation="MESKLTNEQVLEALRAQFATAIGEEEQTIDNYLTVYINSKNVHE
                     IVDWLYKHPEFKIQFLTNITGVHYPENEKGKELMVVYHLHSLQNNFRIRLKAFLPIEN
                     PVIDSIVDIYSAANWIERETYDFYGIIFNNHPNLKRILNQDSMDYFPLRKEYHVEDAT
                     RQDKDDRFFGR"
     gene            complement(1557..2117)
                     /locus_tag="PKN22_10550"
     CDS             complement(1557..2117)
                     /locus_tag="PKN22_10550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNS43188.1"
                     /translation="MGKVTETGQMEAINYEGFQLTKLDTVIGAARANSLWPLPFATSC
                     CGIEYMAAMSPNYDIARFGMERMSFSPRQADLLIIAGTISKKLAPVLRQVYEQMSEPK
                     WVMAVGACASSGGIFDTYSVLQGVDRIIPVDVYVPGCAPRPEQIIDGIMNVQALAKHE
                     SLRRRYSPEYKHLLDNYKIDNDIDGE"
BASE COUNT          659 a          443 c          343 g          709 t
ORIGIN      
        1 acttcaccaa caggataagg catttcgcca taaacttgtt taaagtgatt gatcattcct
       61 tcaatggtat tgtacacatt ttcttttggt ggcaaataaa acaaaggcgt atctgcatga
      121 taaacacctt cttttggcaa gttttcatac gcttggttaa taatacgaat actttgacgt
      181 acttcttctt ggcgtacttg gaaacggtca taagtatcac cttcagttcc aacaggaatg
      241 tcaaaatcaa aatcctcata agaacaataa ggcgaatgca cacgcacgtc ataatcaata
      301 cctgtagcac gcaagttagg gcctacaaat ccatagctca atgctcgttc agcagaaatt
      361 ttccccactc cgatggtacg gtccatgaaa attctatttc tatccaaaag ggcgttgtat
      421 tcttcaaatc gttttaaaaa cgtattgagg aaatcttgaa ttaatgcatg gaatttatca
      481 ttcaaatctc tttcaaaacc accaatacgg ccgatgttgg ttgttaaacg agtaccacaa
      541 atttgttcgt acatatcata gatgcgttca cgttcgataa acatccatgt aaaaccagtt
      601 aaaccaccag tatccacacc aattaccgag ttacaaatca agtggtcggc aatacgcgcc
      661 aactccatga taataacacg catgtattgc actctttttg gaatttcaat gccacacaat
      721 ttttcaaccg tcatttgcca tcctatattg tttaatggcg acgaacaata atttaaacgg
      781 tctgttaatg ttgttatttg ttggaacgat ttacgttctg ccattttttc aaatgcacgg
      841 tgtatatatc caacggttgg ttctgcagac aatatttttt caccatcaac tttcaaaata
      901 ttttggaaaa taccatgggt tgcagggtga gttgggccta aattcagcgt tgcgatttgg
      961 ccatccattg tctcggtaga aaatgttctt tcaagaacac cttcattttt gattttagat
     1021 ttttctaagt cttcactcat atctttcaat tctatcttcc gaagaagcga tcatctttat
     1081 cttgacgtgt tgcatcctca acatggtact cttttctcaa aggaaaataa tccattgagt
     1141 cctggttcaa aatacgtttt aaattcgggt gattgttgaa gatgattccg taaaaatcat
     1201 acgtttctct ttcaatccaa ttggcagcag aataaatatc tacaattgaa tcaattacag
     1261 ggttttcaat tggtaagaac gcttttaaac gaattctaaa attgttttgc aaactgtgta
     1321 aatggtacac caccatcaat tctttacctt tttcattttc aggataatgc actcctgtaa
     1381 tgttggttaa aaattggatt ttaaactctg ggtgtttata taaccaatct acaatttcat
     1441 gtacattttt agaattgatg taaacagtta gatagttatc tatcgtttgt tcttcctcac
     1501 caatagctgt agcaaattga gctctaagag cttctaaaac ttgctcattt gtcaatttac
     1561 tctccatcga tatcgttatc tattttatag ttatccaata aatgtttgta ttctggcgaa
     1621 taacgtctac gcaatgactc gtgttttgcc aaggcttgaa cattcataat accatcaata
     1681 atttgttctg gacgaggtgc gcaacctggc acataaacat ccacaggaat aattctatcc
     1741 acaccttgca atacagaata agtatcaaaa ataccacctg atgacgcaca agctccaaca
     1801 gccattaccc atttaggctc tgacatttgt tcgtacactt gacgtaaaac tggagctaat
     1861 ttttttgaaa ttgttccagc aatgattagc aaatctgctt gacgtggtga aaacgacata
     1921 cgttccatcc caaaacgtgc aatatcgtaa ttgggcgaca ttgctgccat atactcaatt
     1981 ccacaacaag atgttgcaaa cggcaaaggc cacaaagaat ttgctcttgc agcaccaatt
     2041 actgtatcta attttgtcaa ttgaaaacct tcataattga tggcttccat ttgacctgtt
     2101 tctgttactt ttcccattct aaagctcctt ttttaagaac gtaaataaat cctg
//
LOCUS       DAOQVQ010000429         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_64499_length_2151_cov_3.930821, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000429 DAOQVQ010000000
VERSION     DAOQVQ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64499_length_2151_cov_3.930821"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKN22_10555"
     CDS             <1..471
                     /locus_tag="PKN22_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNS43189.1"
                     /translation="AIAVGIKESVCDHHQKARVGQVEYLGKRMEEYGIPLVKPFGGHG
                     IFVDAKAFLPHLTQDQFPAQTLAAEIYADSGVRTMERGIVSAGRKQNGENYYPKLELV
                     RFTIPRRVYTQAHMDVIAESTAIVYDRRKKIKGLKMVYEPKYLRFFQARFEKLK"
     gene            484..>2151
                     /locus_tag="PKN22_10560"
     CDS             484..>2151
                     /locus_tag="PKN22_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNS43190.1"
                     /translation="MITSQLISPKSIAIIGASENTSKPGGKVLQNILEGKFKGPVYAV
                     NKKPIDIKGVIYVSEIEQIEQVDLAIISIPASQCLEAIQKLLNKGAKAFIIYSAGFSE
                     AGEEGIAIEKEIVALINKNNATLIGPNCIGVINDVYKGVFTTPVPNYDKLGCELISSS
                     GATAVFIMESALGTGLKFSNIYSIGNASQTGVEEILEYMDQTFDPENSPKVKLLYLEH
                     IKNPFKFIKHATSLRKKGCRIAAIKSGYSEAGGRAASSHTGALATSDTVIRALFKKCG
                     IVYCSSRDELIAVAGVFQNKEVTGKNIAIITHAGGSAVMLTDALTSNGMNVPPFDAKD
                     TKELLAKLNPGSSVSNPIDFLATGTAEQLGEIIDFCDNYKAVDGMVVVFGSPGLFNVK
                     DVYEVLDNKMRKCKKPIFPVLPSLINAEKEIKHFLSKGRVNFPDEVNLGKALAHVYKS
                     TEISFDKVKLPEMDLPAIRSLVNAASNGFLNQDACRELLEAAGIELVKQQEYTDSQQL
                     ATLKDNMRFPIVMKVTGPVHKTEVGGVILNIHDENQAMDAFNQLMKIP"
BASE COUNT          724 a          400 c          445 g          582 t
ORIGIN      
        1 gccattgccg ttggtatcaa agaaagtgta tgtgatcatc accaaaaagc aagagttggt
       61 caggttgaat accttggaaa acgcatggaa gaatacggta ttccgttggt taaaccattt
      121 ggtggacacg gaatttttgt ggatgcaaaa gcatttttac cacacttaac ccaagatcaa
      181 ttcccagcac aaacattggc agctgaaatt tatgctgatt cgggagtgcg caccatggaa
      241 agaggaattg tttctgccgg aagaaaacaa aatggcgaaa actattatcc aaaattagag
      301 ttggttcgtt ttactattcc acgtcgtgtg tacactcaag cacacatgga tgtaattgca
      361 gaatctacag caatagttta cgacagaaga aagaaaatca aaggattgaa aatggtttac
      421 gagccaaaat acttgcgttt cttccaagca cgatttgaaa aacttaaata gaaaaatttt
      481 tatatgatta cgtctcaact tatttcgcca aaaagcatcg caattattgg tgcatctgaa
      541 aacacaagca aacctggagg taaagtgttg caaaatatat tggaaggaaa atttaaaggt
      601 ccagtttatg ctgtcaacaa aaaaccaatt gacataaaag gtgtaatcta tgtttcagaa
      661 attgagcaaa ttgaacaagt tgatttagca attatttcca ttccggcaag ccagtgtttg
      721 gaagcaattc aaaagctact caacaaaggt gcaaaggcat tcattattta ttctgctgga
      781 ttttctgaag caggcgaaga gggaattgcc attgagaaag aaattgtggc gttaatcaat
      841 aaaaacaatg caacattgat tggtccaaac tgtattggcg ttatcaatga tgtgtataaa
      901 ggtgtgttta caacgcccgt tccaaattac gacaaattgg gctgcgaatt gatttcaagt
      961 tcgggtgcaa ctgccgtttt tattatggaa tcggctttgg gaactggact taaattctcc
     1021 aatatctact ccatcggaaa tgcttcgcaa actggtgttg aagaaatttt ggagtacatg
     1081 gatcaaacgt tcgaccccga aaatagtccg aaagtaaagc tgttgtactt agaacacatc
     1141 aaaaatccat ttaagtttat taaacatgca acttcattaa gaaaaaaagg ttgtcgtatt
     1201 gcggcaatca aatcaggata ctccgaggcg ggaggaagag cggcttcctc ccacactgga
     1261 gcacttgcaa catcagatac cgttattcgt gctttgttta aaaaatgtgg catcgtttat
     1321 tgcagtagtc gagatgaatt aattgccgtt gcaggagttt tccaaaacaa agaagtcaca
     1381 ggaaaaaata ttgccatcat cacacacgcc ggcggttcgg cagtaatgct tacagatgct
     1441 cttacatcca atggaatgaa tgttcctcca ttcgatgcaa aagacaccaa agaattattg
     1501 gctaaactca accctggctc atcggtgagc aaccctattg atttcttggc aactggaaca
     1561 gcagagcaat tgggagaaat cattgatttt tgcgacaatt acaaggccgt tgatggaatg
     1621 gtagttgtat ttggtagtcc cggattgttc aacgtaaaag acgtatacga agtgttggac
     1681 aacaagatga gaaaatgcaa aaagccaatt ttccctgttc tcccatccct catcaatgca
     1741 gaaaaagaaa ttaaacattt cttatccaaa gggcgtgtta actttccaga tgaagtaaac
     1801 ttaggaaagg cgctggcaca cgtttacaaa tcaacagaaa tttcgtttga caaagttaaa
     1861 cttccagaaa tggatttacc tgccattcgc tcattggtga atgccgcttc taatggattt
     1921 ttaaaccaag atgcttgtcg cgaattgctc gaagctgctg gtattgaatt ggtgaaacaa
     1981 caggaataca ccgacagcca acagctggca acattgaaag acaacatgcg ctttccaatt
     2041 gttatgaaag ttaccggacc tgtacataaa acagaagttg gtggtgttat tctcaacatt
     2101 cacgatgaaa accaagcaat ggacgcattc aaccaattga tgaaaattcc a
//
LOCUS       DAOQVQ010000430         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_64876_length_2143_cov_4.135057, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000430 DAOQVQ010000000
VERSION     DAOQVQ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64876_length_2143_cov_4.135057"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKN22_10565"
     CDS             <1..1113
                     /locus_tag="PKN22_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNS43191.1"
                     /translation="LRYTDPNNTQAFASKEAVDYWKNVDLYMGGSEHATGHLLYARFW
                     TKFLFDCGYIPFDEPFKKMINQGMILGRSSFVYRNEQGKYVSKNKLTGNETPIHVDIS
                     LVDNDKLDTEGFKKWRADFANAEFILEDDGSYVCGFEIEKMSKSKYNVQTPDELVEKF
                     GADTLRCYEMFLGPVEQAKPWDTKGINGVSNFLRKLWRLFQIDENGLAHLSTDAPNKE
                     SLKSLHKTIKKVSEDLERFSFNTVVSNFMICVNELTEQKCNNKAILEQLVVLVSPYAP
                     HIAEELWEKMGNGTTSVTQAQFPTFNEEFLVESTIEYPVSFNGKMRFKAALSATLSPK
                     EVEEIVINSPEAAKYLEGKTPKKVIVVPGKIVNVVL"
     gene            complement(1220..>2143)
                     /locus_tag="PKN22_10570"
     CDS             complement(1220..>2143)
                     /locus_tag="PKN22_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43192.1"
                     /translation="SISIENPEFYTFTANATSINFSLCGGCSGSGVQLAIFEIPSGSS
                     CGSGSVNLIYGIIQVTNGISTSSLCGDGSLSVTNSGSLSCVSTTVSGLVVGKKYYIMI
                     DGYKVSGVGQCPFTLTFSSGISPLSVELLSFTGTPTDLGNELNWVTNTEKNNDYFEVE
                     ATLDGVNFETVGRVKGAGNSTKKLTYSLLDRKPLGATTYYRLKQVDFDGTVTHHDLIT
                     VTRTMKNQINLFPNPVNDELTVDFNAEMDGTYILSFVGVSGNVVEHPVTLNKGYNRLH
                     LPTSELAKGFYLLKVADENGNTLSTNKVVKE"
BASE COUNT          705 a          383 c          408 g          647 t
ORIGIN      
        1 ttgcgctata ccgacccaaa caatacccaa gcgtttgctt ccaaagaagc ggttgattat
       61 tggaaaaacg tagatttata catgggcgga tcggagcacg caaccggaca cttgttgtat
      121 gcgcgttttt ggactaaatt tttgtttgat tgtggctaca ttccatttga tgaacctttc
      181 aaaaaaatga ttaatcaagg aatgatcttg ggaagaagtt cttttgttta ccgcaacgag
      241 caagggaaat acgtttcaaa aaataagttg acgggcaatg aaactcctat ccacgttgat
      301 atttctttgg ttgacaacga taaattagac acagaaggat ttaaaaaatg gcgtgctgat
      361 tttgccaatg ccgaatttat tttagaggat gatggcagtt atgtttgtgg ttttgagatt
      421 gaaaaaatgt cgaaatctaa atacaacgtg caaactcccg atgaattggt agagaaattt
      481 ggtgccgata cattgcgttg ttacgaaatg tttttaggtc cggtggaaca agcaaaaccg
      541 tgggatacaa aaggtatcaa cggggtgagt aatttcttgc gtaaattgtg gcgtttgttc
      601 caaatagacg aaaatggatt agcacatctt tctaccgatg caccgaacaa agaaagtttg
      661 aaaagcttac acaaaaccat taaaaaggta agtgaagatt tagaacgttt ttcgttcaat
      721 acggttgtgt ctaattttat gatttgtgtg aacgagttga ccgaacaaaa atgcaacaac
      781 aaagccattt tagaacaatt ggttgttttg gtttcaccgt atgcgccaca tattgcagaa
      841 gaattgtggg agaaaatggg caacggaaca acatcggtaa cgcaagcaca attccctaca
      901 tttaacgaag aatttttggt agaaagcacc atcgaatatc ctgtttcatt taacggaaaa
      961 atgcgtttca aagcggcgtt atcggctacg cttagtccaa aagaagtgga agaaattgta
     1021 ataaattcgc ccgaagcagc taaatatttg gaggggaaaa cacctaaaaa agtgattgta
     1081 gtgccaggaa aaattgtaaa cgtagtactt taagcaagtt gcaatttggt tgagcaaata
     1141 acaaattagt tcaaaaatac ccaacaaaaa acccgttcca acaaacagaa cgggtttttt
     1201 tgttattctt tggtaagaat tattctttta ccactttatt ggtactcaat gtatttccat
     1261 tttcatctgc cactttaagc aaatagaatc cttttgccaa ttcactcgtt ggtaaatgca
     1321 atctgttgta gcctttattg agtgttactg ggtgttcaac cacatttcca gacactccaa
     1381 caaatgaaag aatgtaagtt ccatccattt cggcattaaa atcaacagtt agttcatcgt
     1441 ttacagggtt tggaaacaag ttgatttgat ttttcatggt gcgtgttacc gtaattaaat
     1501 cgtggtgcgt tactgttccg tcaaaatcta cttgtttaag gcgataataa gttgttgcgc
     1561 ccaatggttt tctatccaac aatgaataag ttaatttttt agtagaattt ccagcgcctt
     1621 tcacacgtcc cactgtttca aagttcacac catccaacgt tgcttctacc tcaaaataat
     1681 cgttgttttt ctcggtattg gtcacccaat tcaactcatt tcctaagtct gtaggcgttc
     1741 ctgtgaaaga aagaagttcg acagagaggg gtgatatacc tgaagaaaat gttaaagtaa
     1801 atggacattg tccaacacca ctaactttat aaccatctat catgatataa tatttttttc
     1861 ctacaacaag cccactaaca gttgtagata cacatgataa tgaaccactg tttgttactg
     1921 aaagacttcc atctccacaa agcgatgatg tacttattcc attagtaact tgaataatac
     1981 cataaattaa attaactgat ccacttccac atgaagagcc tgatggaatt tcaaaaatag
     2041 ctaattgtac tccgctacca ctacaaccac cacaaagaga aaagttgata gaagttgcat
     2101 tagcagtaaa tgtgtaaaac tctggatttt caattgaaat act
//
LOCUS       DAOQVQ010000431         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_65028_length_2140_cov_3.031175, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000431 DAOQVQ010000000
VERSION     DAOQVQ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65028_length_2140_cov_3.031175"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2140
                     /locus_tag="PKN22_10575"
     CDS             <1..>2140
                     /locus_tag="PKN22_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43193.1"
                     /translation="EMYAGGISFGAGYPITTGAFDASFNGGNSNMNIAGFDVSISKFS
                     SDGTQLLFSTYLGGNANELPQSMIATHTGDLYVLGITASANFPLGATPYQSTFNGGAT
                     TVENSLKFNGSDFYIVKFNASGTQLLASTYVGGSSIDGLNTSTLKFNYGDQFRGEIIL
                     HGNDVLVASHTQSADFPVTNGSTLNGSQDIVVFSLNSDLTQLLWSSYFGGSGVETGNS
                     IALSSAGEAFVTGGTSSSDFTLTGFDPSFNGDRDGYILKLNATTGAVLAGTYLGQSEY
                     DQSYFVSLDIDDFVYVFGQSESAWTISAGKYGNANSGQFIQKYNNALTAVQWTTMIGA
                     GTGHPEISPTAFLISDCYDIFFAGWGGVVNVNGSQATNSSSNGFPVTSDAYQSTTNGS
                     NFYLAILSQDATNLVYATYMGGTTGYYNHVDGGTCRFDKSGAVYHAVCAACSGIDNGF
                     TTTPGVWSTTNNSPNCNLAAFKFQLGMPYSLSANTSVCNGGSIQLNATGGIGYTWTPA
                     ASLNNPNIPNPIATPTETTVYHVSMNFNAGCAIDDSIIIEVINEPIIDLADAVSICKN
                     DSITITASGGTTYSWSPNQYINNTTSATVKVSPPNSMYYYVTVGNECFERTDSIYVTV
                     NPLPEIILVDDTTICKGAGVQLVPDGNMQPTWLPHSTLVANTDGSATATPTIPQYYYV
                     TGTDANGCHNKDSVKVEFYDIPTLTLSPDTT"
BASE COUNT          612 a          450 c          450 g          628 t
ORIGIN      
        1 agaaatgtat gccggtggaa ttagttttgg agctggctac cctattacaa ctggcgcttt
       61 tgacgcttcg tttaacggag gaaattccaa catgaacatt gctgggtttg atgtgtcaat
      121 ttctaaattt agtagcgatg gcacacaatt gttgttttcc acgtatttgg gcggaaacgc
      181 caatgagttg ccacaaagta tgattgctac acacaccggc gatttgtatg tgctaggcat
      241 tacggcatct gccaatttcc cgttgggagc aacgccttat caaagcacct ttaatggtgg
      301 tgcaacaacc gttgaaaact ccttaaaatt caacggttct gatttttata ttgtaaaatt
      361 caacgcatcg ggaacacaac tattggcttc gacttatgtt ggtggttcta gcattgatgg
      421 actaaacacc agtactttga aattcaacta cggcgatcaa tttagaggcg aaatcatcct
      481 tcatggcaat gatgtattgg tagcttcgca tacccaatcg gctgattttc ctgtaacaaa
      541 tggttccaca ttaaacggct cgcaagatat tgttgtgttt agtttaaata gtgatttaac
      601 ccaactgttg tggtcatctt attttggtgg ctctggcgtt gaaacaggaa attccattgc
      661 actttcatct gcaggagagg catttgtaac aggaggaacg tcttcatcgg attttacatt
      721 aacaggattc gatccttcat ttaacggcga tagggatggt tatattttaa agttgaatgc
      781 cacaactggt gcggtattgg caggaactta tttaggacaa agcgaatacg atcaaagtta
      841 ttttgtgagt ttagacatcg atgattttgt gtatgttttt ggacaaagtg aatccgcatg
      901 gaccatcagc gcaggcaaat atggcaatgc caattcggga caatttatcc aaaaatacaa
      961 caatgcacta actgctgtgc aatggacaac catgataggt gcaggcactg gacatcccga
     1021 aatttctccc acggcgtttt taatttctga ctgctacgat attttctttg ccggttgggg
     1081 aggtgttgtg aatgtgaatg gaagtcaggc tacaaatagt tcatctaatg gatttcctgt
     1141 gacatcagac gcctatcaat ctacaaccaa cgggtcaaat ttttatttag ccattttaag
     1201 tcaagatgct accaatttgg tgtacgcaac ttatatgggt ggcacaacag gatattacaa
     1261 ccacgtggat ggaggtactt gccgttttga taaatcggga gcagtttacc atgcggtgtg
     1321 tgctgcttgt tcgggaattg acaatggttt tacaactact ccaggagttt ggtcaaccac
     1381 caacaacagc cccaattgca acctagctgc gtttaaattt cagttgggta tgccctattc
     1441 gttgagcgcc aacacttctg tttgcaacgg aggttctatt caactcaacg caacaggagg
     1501 aattggttat acatggacac ctgctgcttc attgaataat cccaatattc ccaatcctat
     1561 agctacacca acagaaacta ctgtttatca cgtgagcatg aattttaatg cgggttgtgc
     1621 tattgatgat tccatcatta ttgaagttat aaacgaacca atcattgacc tagcagatgc
     1681 cgtttcgatt tgtaaaaacg atagcatcac cattaccgct tctggaggga caacctatag
     1741 ctggtcgcct aatcaataca tcaacaatac aactagtgcc accgttaaag tttcacctcc
     1801 aaacagcatg tattactacg taacggttgg caacgaatgc tttgagcgca ccgatagtat
     1861 ttatgtaacc gtcaaccctt tgcctgaaat aattttggtg gacgacacca ctatttgcaa
     1921 gggtgcaggc gttcaactag tacctgatgg caatatgcaa ccaacgtggc taccacattc
     1981 tactctcgtg gcaaataccg atggaagtgc tactgcaaca cctacaattc cacaatatta
     2041 ctacgtaaca ggaacggatg ccaacggttg ccacaacaaa gattctgtaa aagtggagtt
     2101 ttatgatatc ccaactttaa cactttctcc tgacaccaca
//
LOCUS       DAOQVQ010000432         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_65154_length_2137_cov_4.153218, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000432 DAOQVQ010000000
VERSION     DAOQVQ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65154_length_2137_cov_4.153218"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKN22_10580"
     CDS             <1..752
                     /locus_tag="PKN22_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNS43194.1"
                     /translation="CAEIIERANPDIIDINYGCPVKKVACKGAGAGILQDVPKMVKMT
                     EAIVKQVKLPVTVKTRLGWDDNTKYIVEVAERLQDVGIEAISIHGRTRKQMYKGQADW
                     TLIEQVKNNQRMHIPVFGNGDIDSPEKALEYKNRYGVDGIMIGRASIGYPWIFNEIKH
                     YLKTGEHLAKPTIQDRVEAARRHLDMSIKWKGQALGIAEMKRHYTNYFKGIAHFKEYR
                     MKLVTSFNLEEIYQTLDEITAHMDDFHFAHE"
     gene            complement(843..1895)
                     /gene="queA"
                     /locus_tag="PKN22_10585"
     CDS             complement(843..1895)
                     /gene="queA"
                     /locus_tag="PKN22_10585"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNS43195.1"
                     /translation="MKLSEFNFDLPQELIAEHPSENRDEARLMVVHRKTGKIEHKLFK
                     DLVNYFSDGDVMIRNNTRVFPARMYGNKEKTGAKIEVFLLRELNRESLLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLFDGPYEEFKAKITELGETPLPKYI
                     KRAVEPEDEERYQTIYATEEGAVAAPTAGLHFSRELLKRLEIKGVNFAEVTLHIGLGT
                     FRTVEVEDLTKHKMDSEQVIITQKAADIVNNAIENKKRVCAIGTTSMRAIESSVSTDG
                     KLKAFDGWTNKFIFPPYDFSIANCMITNFHTPLSTLLMMISAFAGHEVMTKAYKAAVE
                     EKYKFYSYGDAMLIID"
BASE COUNT          667 a          378 c          407 g          685 t
ORIGIN      
        1 aatgtgctga aatcatagag cgagcaaatc cagatatcat cgacattaac tatggttgtc
       61 cggtaaaaaa agttgcctgc aaaggtgctg gtgctggaat tttgcaagac gtacctaaaa
      121 tggtgaaaat gaccgaagcc attgtgaaac aagtgaaatt accggttact gtgaaaacac
      181 gtttaggttg ggacgataac acaaaataca ttgtggaagt ggcagaacgc ttgcaagatg
      241 taggtattga ggcaattagt attcatggtc gcacacgcaa gcagatgtat aaggggcaag
      301 ccgattggac cttaattgaa caagtgaaaa ataaccaacg tatgcatatt cctgtgtttg
      361 gaaacggtga tattgactct ccagaaaaag cgttggaata taaaaatcgg tatggtgtag
      421 atggtattat gattggtcgc gcatcaattg gttatccgtg gatttttaat gaaatcaagc
      481 attatttaaa aacaggagag catttagcaa aacctactat tcaagataga gtggaggctg
      541 ctcgacgcca tttagacatg agcatcaagt ggaaaggcca agcattggga attgccgaga
      601 tgaaacgcca ttataccaat tatttcaagg gaattgcgca tttcaaagaa taccgcatga
      661 agttggtaac atcgtttaat ttagaagaga tttaccaaac gttggatgaa atcacggcgc
      721 acatggatga ttttcatttt gctcacgaat aaattagtaa aatcttagca tacaaaaaag
      781 aggttagtca caatggctaa cctcttttct catagttgtg tttctagttt ttagctagat
      841 ttttaatcaa ttatcaacat agcatcaccg tatgaataga atttgtattt ttcttctacg
      901 gctgctttgt aagcttttgt cattacttcg tgaccggcaa aagcggatat catcatcaat
      961 aaagttgaaa gcggtgtgtg gaaattggta atcatacaat ttgcgatact gaaatcgtat
     1021 ggcgggaaga tgaatttgtt ggtccaaccg tcaaaagctt tcaatttacc atctgttgaa
     1081 acagaagatt caatggctct catggaagtt gttccaatag cgcaaacacg ctttttattt
     1141 tcaatagcat tgttaacgat gtcggcagct ttttgcgtaa tgataacttg ctccgaatcc
     1201 attttatgtt tcgttaagtc ttccacttca acggtgcgga aagttcctaa accaatgtgt
     1261 agtgttactt ctgcaaagtt aactccttta atttcaagtc gttttaataa ttcgcgcgaa
     1321 aaatgcaatc ctgcagtagg tgctgctaca gctccctctt cagttgcata aattgtttgg
     1381 taacgctctt cgtcttcagg ttcaaccgct cttttgatgt atttaggaag aggtgtttca
     1441 cccaattctg taatttttgc tttgaactct tcgtaaggtc cgtcaaaaag aaaacgcaat
     1501 gtacgacctc tagaagttgt attatcaatt acttcggcaa ccaatgaatc gtcatcaccg
     1561 aaatacaatt tattaccaat tctaattttt ctagctggat ctaccaaaac atcccacaac
     1621 aagctttcgc ggttcaattc gcgcaataaa aacacctcga ttttagcacc agttttttcc
     1681 ttgtttccat acatacgtgc aggaaacacg cgggtgttgt tgcgaatcat tacatcgcca
     1741 tccgagaaat aattaactaa atctttaaaa agtttgtgtt caatttttcc tgttttacgg
     1801 tgcaccacca tcaaacgtgc ttcatcacgg ttttcagaag ggtgttcggc gattaattct
     1861 tgaggtaaat cgaaattaaa ttccgatagt ttcattttac tcattgcaaa caaatgttta
     1921 ttttaactaa aaaagagcac aaagttaaat aaaattagca agtaaattta gattttaact
     1981 aattctttaa aaattgtttt ttccagctat tgtatttttt tgattttatt tgatctagag
     2041 tattataaaa tgtttttgcc cagtagtttc ttagaccaaa tccacctgct ttttctttaa
     2101 aaacatcgta gagtaaaatg gcattgtttt tcatatt
//
LOCUS       DAOQVQ010000433         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_65231_length_2136_cov_2.538683, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000433 DAOQVQ010000000
VERSION     DAOQVQ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65231_length_2136_cov_2.538683"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1616
                     /locus_tag="PKN22_10590"
     CDS             345..1616
                     /locus_tag="PKN22_10590"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNS43196.1"
                     /translation="MTEQFLQYVWFQKLFSPLQVTSQGESVEIIDVGQLNTDAGPDVF
                     NAKVKINNTLWAGNVEFHQKSSDWARHGHTQNPAYNSVILHVVFDIDAIIYRADGSEM
                     PQIRLNFSPHLLEQYEVLQQQNSFVACEKQLGKVSSVHLHNWLDRLLVERLEQKTHVI
                     ETLLNQTTNNWEEVFYIVLARNFGFSTNALAFELLAKSLPLNILAKHKNNLMQLEALL
                     FGQAGLLEKPNTDDAYVTQLKNEYHFLRTKYQLEPIDYSLWKLLRLRPANFPTVRLAQ
                     FATLIHQSSKLFSKILETTSYNDLRPLFQCEVSDYWLTHYLFGESSKLVSKKLSKSSI
                     DSLLINTVVPFLFHYGKVNNNEELVQRSFDLLSTITPEKNHIVTGFENCGIKSQSAYD
                     SQALIQLKRGYCDLHKCLQCHISKQVLYAKL"
     gene            1695..>2136
                     /locus_tag="PKN22_10595"
     CDS             1695..>2136
                     /locus_tag="PKN22_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43197.1"
                     /translation="MEKKITVFGVIGEGFSIGMKNSLSLLGAVILYALTIWIPYLNVG
                     TTIAMCSIPVELSKGKVISPFFIFDAKYRKYMGEFFLLLGFVSIAVVPLILFGFFPAI
                     VISLAWSLAVFLLLDKGVSPGEALIQSNKLTYGYKWTLFFISLVL"
BASE COUNT          625 a          353 c          398 g          760 t
ORIGIN      
        1 cgaggcgatg ccataaccca actgaaagcc ttcaacaaag gacatgattg tgttctggaa
       61 aacagcaatc aaaaagctga ccaaaagcaa ggtgcataga attttaaaac gtttcatagg
      121 cgtttatggt ttaaaagtgt ttataatttc gatgcaaagg aaatgattaa tttttgaata
      181 accaaatatt tttatcgttt ttcgatattt ttttattgaa aaagtttgtt ttacccttaa
      241 ctattataaa atatgtgaaa atcaattgcg taaaattgtc ttaaattgtg atatatgttt
      301 gttaaaatat atacctttgt cactactcgt taaattaaaa gcctatgacc gagcaatttt
      361 tacagtatgt ttggtttcaa aaattgtttt cgcctttgca agttacttcg cagggtgaat
      421 ctgttgaaat tattgatgtg ggacaactca ataccgatgc aggtcccgat gtgtttaatg
      481 ccaaggtaaa gataaacaac acattatggg ctggcaatgt ggaatttcat caaaaatcga
      541 gcgactgggc acgtcatggg cacacccaaa accctgctta caattctgta attttacatg
      601 ttgtgttcga tattgatgcc attatttatc gtgccgatgg tagtgaaatg ccacaaatac
      661 gtttgaactt ttctcctcat ttgctggaac aatacgaagt tttacaacaa caaaactcat
      721 ttgtggcgtg tgagaaacaa ttgggcaagg tgtcatcggt tcatcttcac aattggctcg
      781 atcgtttatt ggtagagcgt ttggaacaga aaactcacgt gattgaaacg ttattaaacc
      841 aaactactaa caattgggaa gaggtttttt atatcgtttt ggctcgtaat tttggttttt
      901 ccactaatgc gttagcgttt gaattgctcg ccaaatcgtt accgttaaat attttagcaa
      961 aacacaaaaa taacttgatg cagctcgaag ctttgttgtt tggtcaagct ggcttgcttg
     1021 aaaaacccaa tactgatgat gcatatgtta ctcaattaaa gaacgaatat cactttctgc
     1081 gcaccaaata tcagttagag ccgattgatt attcgttgtg gaaactattg cgtttgcgtc
     1141 ctgctaattt tccaactgtg cgcttggcac aattcgccac cttgattcac caatcgagta
     1201 aattattttc taaaattctc gaaacaactt cttataatga cttgcgtccg ctgtttcaat
     1261 gtgaagtgtc cgattattgg ttgacgcatt atttgtttgg cgaatcgagt aagttggtgt
     1321 ccaaaaagtt gtcaaaatct tccattgata gtttactgat taacacggtc gttccgtttt
     1381 tatttcatta tggtaaagta aataataatg aggaacttgt acaacgttct tttgatttgc
     1441 taagtactat tacgccagag aaaaatcaca tagtgactgg attcgaaaat tgtggtatta
     1501 aatctcagtc agcctacgat tcacaggcac ttattcaatt aaaacgaggt tactgcgact
     1561 tgcataaatg cctgcaatgt catatatcaa aacaggtgct ttatgcaaaa ttataactaa
     1621 attgtttttt ttagtaattt tttactactt ttgtctcatt ggtatttaat tattcatctt
     1681 aaaatcattt tgttatggaa aagaaaatta ccgtttttgg cgttattggc gagggttttt
     1741 ccattggtat gaaaaactca ttgtcattgc taggtgctgt cattttgtac gcacttacta
     1801 tttggattcc ttaccttaat gtgggaacaa ccattgctat gtgttctatc cctgtagaat
     1861 tgagcaaagg aaaagttatt tctccatttt tcattttcga tgcaaaatat cgaaaataca
     1921 tgggcgaatt tttcttattg ttgggatttg tttctattgc cgtagtgcca ttgattttat
     1981 ttggattttt tccagctatc gttatctctt tagcatggtc gttagctgtt tttctcttgc
     2041 ttgataaagg cgtttcgcca ggtgaagcgt tgatacaaag taataagttg acttacggtt
     2101 ataaatggac attgtttttc atttcgcttg tgttgg
//
LOCUS       DAOQVQ010000434         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_65354_length_2133_cov_3.837825, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000434 DAOQVQ010000000
VERSION     DAOQVQ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65354_length_2133_cov_3.837825"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKN22_10600"
     CDS             <1..924
                     /locus_tag="PKN22_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNS43198.1"
                     /translation="MLGLAFSEESLEVQKNVVIEEFKQRNLNQPYGDVSKLLRELAYK
                     VHPYKWATIGKEIAHIENATMDDVKAFFNKYYNPSNAILCIAGNFELEEIKRLVEKWY
                     GDIPAGKKFARILKKEPEQTEYRELTIERDVPANAFYYAFKMPERRNPEYYVADVISD
                     ELGRDKSSILYKKLKKELELVTEIAAYITGSIDEGLLIISGRLAENVSFEQLDTALWS
                     VLERFKNKQIDAETLKRLMIKIRTAKEFQDQSMLNKAMNLCFFELLGDANGINEEWLV
                     YNQIEPSDIQQTAKQLLKKTNCSLLKVQVKK"
     gene            complement(934..>2133)
                     /locus_tag="PKN22_10605"
     CDS             complement(934..>2133)
                     /locus_tag="PKN22_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNS43199.1"
                     /translation="LFEKSFVDLTRQANSKPRFDVFLTVSTHHPFLIPEQKRYLKIVQ
                     QRFKHVEKLKQQQEVALKKPESLATYAYTDDALRAYFEKVKKLPDFNNTIFIIFGDHG
                     NHDLLFSGLDRYKTSMLIYSPLIKQPKTIRSVSTHLDIAPSILNFLRINYPAMQLPSR
                     TPFYGGELDLNPNYECKRTLPLNTIQHENSHLVMGKYLVMDNVLYKLSNNLTPQVYRN
                     ENKQKQLEKQLELYNRLVKYQFNWNLLLPETLFDLYVEQTYFKLIYENKVVDDKQNLK
                     SEFINIGNYPIANPKWKKMRLECELDAVLKDETYLDSIPKLTCSIENINNDGKKEVIV
                     WKNITPRILQKFNKNSWNKITYVLDVDFRNIPKVNKNNQFSFYIYNVEKKPQQMKNNS
                     FKLFAAD"
BASE COUNT          681 a          311 c          419 g          722 t
ORIGIN      
        1 atgttgggct tggctttttc ggaagagtcg ctggaggtac agaagaatgt ggttattgaa
       61 gagtttaaac agcgcaactt aaaccagcct tacggggatg taagcaaatt gttgcgtgag
      121 cttgcctata aagtacatcc gtataaatgg gcaacgatag gtaaagaaat tgcgcacatt
      181 gaaaatgcta cgatggacga tgtaaaagca ttttttaata agtattacaa tccttcgaat
      241 gcaattttgt gcatagctgg taattttgaa ctagaggaaa ttaaacgctt ggttgaaaaa
      301 tggtatggcg atattccggc gggtaaaaag tttgcacgca ttctaaaaaa agaaccagaa
      361 caaacagagt acagagaatt aactatagag cgcgatgttc ctgccaatgc tttttattat
      421 gcctttaaaa tgcccgaacg tagaaatcca gaatactatg ttgccgatgt aatttcagat
      481 gaattgggac gcgataaatc ttctattctc tataaaaagt taaaaaagga attagaatta
      541 gtaaccgaga ttgcggctta tattacgggt tctattgacg aaggcttatt aattatttct
      601 ggtcgattgg cagaaaacgt atcgtttgaa caattggaca cagctttatg gtcggtgttg
      661 gagcgcttta agaacaaaca aattgatgct gagacattaa aacgattgat gataaaaatt
      721 cgtactgcta aagagtttca agatcaatcc atgttgaaca aagcaatgaa tttgtgtttc
      781 tttgaattgt tgggcgatgc caatggaatt aatgaagaat ggttggttta caatcaaata
      841 gagccaagtg atattcaaca aacagccaag cagttattga aaaaaacaaa ttgttctttg
      901 ttgaaggtgc aagtgaaaaa atgaggttga tttttaatca gccgcaaata gtttgaatga
      961 attatttttc atttgttgcg gttttttctc aacattgtaa atgtaaaacg aaaactgatt
     1021 gtttttgttc acttttggaa tatttctaaa atctacatcc agcacgtagg taattttatt
     1081 ccaactgttt ttattgaact tctgcagaat tcgaggggtg atgtttttcc aaacaataac
     1141 ttctttctta ccatcattgt tgatgttttc gatagaacaa gttagtttag ggatggaatc
     1201 taagtatgtt tcatctttta aaacagcatc taattcacat tcaagtcgca tttttttcca
     1261 ctttggattg gcaataggat aatttccgat gttgatgaat tcagatttta aattttgttt
     1321 gtcgtcaaca accttgtttt cataaattaa tttgaaataa gtttgttcaa cgtataagtc
     1381 aaacaatgtt tcgggcaata acaaattcca attaaattga tattttacca aacggttgta
     1441 taattctaac tgtttttcta actgtttttg tttgttctca ttacgataaa cttgaggtgt
     1501 taaattattg gaaagcttgt atagtacgtt atccattact agatatttgc ccattaccaa
     1561 atgcgaattc tcgtgctgaa tagtatttaa tggcaaggtt cttttacatt cgtagttggg
     1621 atttaaatcc aattcgcctc cataaaacgg agttcttgat ggcaattgca tggctggata
     1681 attgatgcgt aaaaaattca atatgctcgg tgcaatatcc aaatgggtgg aaacagatcg
     1741 gatggttttg ggctgtttta tcaatggtga ataaatcaac atactggtct tgtaacggtc
     1801 taagccagaa aacaacaaat cgtgattgcc atgatcacca aaaatgatga aaatggtgtt
     1861 gttaaaatcg ggtaatttct taactttttc aaaataagct ctaagcgcat cgtctgtata
     1921 agcataggtg gcaagcgatt ctggcttttt gagtgctact tcttgttgtt gttttaattt
     1981 ttctacatgt ttaaatcgct gttgaacaat ttttaaatag cgtttttgct ctggaattaa
     2041 aaatggatgg tgcgtgctta ctgttaaaaa aacatcaaat cgcggttttg aatttgcttg
     2101 acgcgttaaa tctacaaacg atttttcaaa taa
//
LOCUS       DAOQVQ010000435         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_65624_length_2127_cov_3.722490, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000435 DAOQVQ010000000
VERSION     DAOQVQ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65624_length_2127_cov_3.722490"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKN22_10610"
     CDS             complement(<1..423)
                     /locus_tag="PKN22_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNS43200.1"
                     /translation="MSEVNEQLKQEQQNLIVFIWKNRKLIIAIAAAGFVVSLVISLLL
                     TPVFKSSAIVFPTATSTVSFSEQRNAKASSMDFGESEQAEQMVQILQSSNIRDKIVSE
                     FDLYKHYEIDDNDPNKHYKLVKTFKENFSFSRTRYGSIA"
     gene            complement(565..>2127)
                     /locus_tag="PKN22_10615"
     CDS             complement(565..>2127)
                     /locus_tag="PKN22_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNS43201.1"
                     /translation="EKENYEAFKALGTLNLKDFKYVSPELTQEVQVNEMQFRFAPQYL
                     ALEKLDAKTGNSDFAMNGTIDNYMGYIFRDELLKGKFTFNSNNLDLDQLMNIVPASAD
                     TTAQQPAAASTTEEAFEIPGNIDFALNTNINKVKFNGMDATNVQGAVTLKDKIATLNN
                     LNLNAMGGSIGLTGQYNTQNPQKPKAAIGYNLKELDIQQLANNFLTIEKLAPIAKYVQ
                     GKISSNFNLSTDLTSNFEPVLNSVNGLGDLLSSSVSISNLKIFDKLEDVTKLANFNNQ
                     TIKNLKASFKIQDGKLAVNPFDILLGGIKTTISGTNGLDQSIDYIMKMNIPKEKLPKE
                     MIKLVEDAVAKVNKLAPKLNLDVIPAQIPISANIIGTFKDPKINTDFKEKLMDLSGNM
                     KDALKDKANEVVNQVKDSVKTVVNNAVEKGKEELEKQKQQILNSAQKQADNVKAEGKK
                     AADKIRSEADKAYQQAVDAVSNPLAKKAAEVTAKKAKDEAYKKAQQAENEANKQADAI
                     MQKARDEADKLK"
BASE COUNT          559 a          385 c          365 g          818 t
ORIGIN      
        1 agcaatggaa ccgtagcgtg tgcgagaaaa actaaaattt tctttgaacg tttttactaa
       61 tttataatgt ttattagggt cgttatcgtc aatttcatag tgtttataca aatcaaattc
      121 acttacaatt ttatcgcgaa tgttggacga ttgtaagatt tgcaccattt gttctgcttg
      181 ctcagattca ccaaagtcca tggatgacgc cttggcattg cgttgttcgg aaaacgaaac
      241 cgtactggtt gcagttggaa acacaatagc cgatgatttg aaaacaggtg tcaacaataa
      301 agaaatcacc aatgaaacca caaaacctgc cgcggcaatt gcgatgatta attttctgtt
      361 tttccagata aaaacgatca gattttgttg ttcttgcttc aattgttcgt taacttcact
      421 catgtatttg taatttacgt ttttttaggt tgcacaaact taattaaaat ttagttgtag
      481 tatataacct gttgatagta atattattgt ttttcatgta aaaaaaaaca gcccgagttt
      541 acctcgagcc gttattgaaa acaattattt taatttatct gcttcatcgc gggctttttg
      601 cataattgca tcggcttgtt tgtttgcctc gttttctgct tgttgcgctt ttttgtaggc
      661 ttcatctttt gcttttttag ctgtaacttc agcggctttt ttagccagtg gattggaaac
      721 tgcatcaacc gcttgttggt atgctttatc ggcttctgag cgaattttat cagcggcttt
      781 tttaccttca gctttcacat tatccgcttg cttttgtgcc gagtttaaga tttgttgttt
      841 ttgtttttcc aactcttctt ttcctttctc aacggcattg ttaacaacgg tttttacaga
      901 atcttttact tgatttacca cttcattggc tttgtctttc aaagcatctt tcatgttgcc
      961 cgacaaatcc attaattttt ctttaaagtc ggtattgatt ttaggatctt taaacgttcc
     1021 aatgatattt gcactaattg gaatttgagc tggaataacg tctaaattca attttggtgc
     1081 aagtttattc acttttgcaa cggcgtcttc taccaattta atcatttctt ttggcaattt
     1141 ttctttcggg atattcattt tcatgatgta atcaatggat tgatccaaac cgttggtgcc
     1201 cgaaatggtt gttttgatgc cacctaacaa aatatcaaat ggatttacag ccaattttcc
     1261 gtcctgaatt ttaaaacttg cttttaaatt tttgattgtt tggttgttga aattggccaa
     1321 tttagtaaca tcttctaact tatcaaagat ttttaaatta gagatagaaa ccgaagaact
     1381 caacaaatca cccaaaccgt ttacagaatt taatactggt tcaaagttgg atgttaaatc
     1441 agttgacaaa ttaaagttgg atgaaatttt accttgcaca tatttagcaa ttggtgccaa
     1501 tttttcaatc gtcaagaagt tgtttgccaa ctgttgaata tccaattctt ttaggttgta
     1561 acctattgct gcttttggtt tttgaggatt ttgcgtgttg tattgccctg ttaatccaat
     1621 acttccgccc attgcattca agttcaaatt attgagagtt gcaattttgt ctttcaatgt
     1681 aactgcacct tgcacgttgg tagcatccat accgttgaat tttactttat tgatgttggt
     1741 atttaacgca aaatcaatgt ttcctggaat ttcaaacgcc tcttctgttg ttgatgcagc
     1801 tgctggttgt tgcgctgttg tatcggcaga agctggcaca atgttcatca attgatctaa
     1861 atccaagttg ttactgttga acgtgaattt tcctttcaac aactcatcac ggaagatgta
     1921 acccatgtag ttatcgattg ttccattcat ggcaaaatca gagttccctg ttttagcgtc
     1981 caatttttcc aacgccaagt attgtggtgc aaatctaaat tgcatttcat tgacttgtac
     2041 ttcttgcgtt aattctggcg aaacgtattt aaaatctttc aaattcaacg ttcccaatgc
     2101 tttaaacgct tcgtagtttt ctttttc
//
LOCUS       DAOQVQ010000436         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66085_length_2118_cov_4.321377, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000436 DAOQVQ010000000
VERSION     DAOQVQ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66085_length_2118_cov_4.321377"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="PKN22_10620"
     CDS             complement(<1..1915)
                     /locus_tag="PKN22_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNS43202.1"
                     /translation="MKLKKITILLVVVTTITTGYSQMTATFNKTINTICNGSGCDYNG
                     PSILINEIQVAPNNGDGSLSGNQSDGIPPLVPPVAAAGEWIELYNPNICEPVDISCYY
                     LGNAANDPSGPYGGGFQLPANTIVPPGGFCVVRGANAPAVPTNLLVANGGNTVEVIVP
                     VSITGNGVCVGSGGHRLWFPNAGGWFAFYDRNGQVQDAISWGDAAGSAGLPCIPTSTG
                     CNTGVTSLASYTNIPANRKQNIYSSAVPKDDLTIRRMPDGGAWATDVKAPATYGTCND
                     VCATLGTSSCDATATINVSGGNAPYSYLWNDSEAQLTQTATGLCAGTYNVTVTDANNA
                     TQTFTVTIENYVPTVTFAPQTSVCNEGQTVALSNYSPTPTSGQTGVFTGTGVSGTSFN
                     VATAGNGTFPIIYTFTDENGCENADTSSITVNPKPVPTISGVDPTYCLSNTTVTPTLS
                     PAGGTLSGPGVTGNTINIFNAGPGTHTIKYVVTNQFGCSDSTTVSFTVTTTTPPTFSV
                     QNEICLGAAPIALIGTPAGGTFNVGTQTITQFDPAQFGLGQHVITYTITDPNNPGCLA
                     QASDTIKVINGGTITTNTPTYFCFNSPDYNVVMNPAGGTLTGSLISGNTLQVANATPG
                     AYSFTYDYTSAQGC"
BASE COUNT          642 a          412 c          414 g          650 t
ORIGIN      
        1 gacaaccttg cgcacttgtg taatcgtagg taaacgaata agcaccaggc gtagcatttg
       61 caacttgcaa tgtatttcca gaaatgaggg aacctgttaa agttccacca gctggattca
      121 taactacgtt ataatctggt gaattgaagc aaaaataggt tggtgtattg gttgtaatcg
      181 ttccaccatt tataacttta attgtatccg aagcttgtgc caaacaccct gggttgttag
      241 gatcggtgat ggtgtaggta atcacgtgtt gtcctaaacc aaactgtgca gggtcaaatt
      301 gtgtgatggt ttgtgttcct acgttaaatg ttccaccagc tggagttcca atcaaagcaa
      361 taggtgctgc tcctaaacaa atttcatttt gtacagaaaa agttggaggc gttgttgtgg
      421 taactgtaaa agaaacagtt gttgaatcac tacatccaaa ttggttggtt acaacatatt
      481 taatggtatg tgttcccggt ccggcattaa aaatattgat ggtatttcca gttactccag
      541 gtccagataa ggtgccacca gcaggtgaca acgtaggcgt aactgttgtg ttagacaagc
      601 aatacgttgg gtcaacacca gaaattgttg gcactggctt tggattgaca gtaatggatg
      661 aagtatcggc attttcacac ccattttcat cggtaaaagt atagatgata gggaaagtac
      721 catttccagc agttgcaacg ttgaaagatg tgccacttac accagttcca gtaaacacac
      781 ccgtttgtcc acttgttgga gtaggtgagt aattggataa agctactgtt tgtccttcat
      841 tacaaacact tgtttgaggg gcaaaagtta cagttggaac ataattttca atggtaacag
      901 taaatgtttg ggtagcattg tttgcatcgg taacagttac gttgtatgtt cccgcacaca
      961 atcctgtagc agtttgtgta agttgcgctt ctgagtcgtt ccaaaggtag ctatacggcg
     1021 catttcctcc gcttacgtta attgtagcag tagcatcaca tgacgatgtt ccgagtgtag
     1081 cacaaacgtc gttgcaagtt ccatacgttg caggagcttt tacatcagtt gcccaagccc
     1141 ctccatctgg cattcttcta attgttaaat catcttttgg cacggcgcta ctataaatat
     1201 tttgttttct atttgcagga atattcgtgt agcttgctaa agaagtcaca cctgtattac
     1261 aacctgtact tgtgggaata caaggtaaac ctgcgcttcc tgcagcatct ccccagctaa
     1321 tagcatcttg tacttgtccg tttctatcat aaaaagcaaa ccaaccacca gcattaggaa
     1381 accataaacg gtgcccacca cttccaacac aaactccatt acctgtaatg ctaacaggca
     1441 caattacctc aacagtattt cctccattag caaccaataa attagttgga acagctggtg
     1501 cattagcacc tcttacaaca caaaacccac ctggaggaac aatcgtattg gcaggaagtt
     1561 gaaacccacc accatacggt ccagaaggat cattggcagc atttcccaaa taataacacg
     1621 aaatatcaac aggttcacaa atatttggat tgtacagttc tatccattct ccagcagctg
     1681 caactggtgg aactaatggt ggaataccat ctgattgatt tccagataaa gatccatcac
     1741 cattattggg tgcaacttga atttcgttaa ttaaaatact tggtccattg taatcacagc
     1801 cactcccatt gcaaatagta ttaattgttt tgttgaacgt cgccgtcatt tgcgaataac
     1861 cagtagttat ggttgtgaca acaacaagta gaattgtaat tttttttagt ttcataatga
     1921 atgaaaaatg ttagtttgtt atattttgag tagtaggtga acgctaaatt gataaatgtg
     1981 gttgcatatt tacacataat atattaaata tcatataatt tgttaaatat ttatcacttt
     2041 taaaatatta taaaactaat taacatgaaa aaagctatcc cgaagaatag cttttagaat
     2101 cattttattg taattatt
//
LOCUS       DAOQVQ010000437         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66216_length_2116_cov_2.984959, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000437 DAOQVQ010000000
VERSION     DAOQVQ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66216_length_2116_cov_2.984959"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="PKN22_10625"
     CDS             complement(<1..2048)
                     /locus_tag="PKN22_10625"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS43203.1"
                     /translation="MFERGKKIDEVTIFAYKDPVHFKGDTLVYIADSFKTRPNAVVED
                     LLKKLPGITVEKNGTIKSQGKDISRIYVDGDEFFGSDATLATKNLAANSVERVQVYET
                     HLPDASSTDEKVKVLNLELKDAAKKGYFGKVAFGTDFMNYFEGQALFNKFSKDHKIAA
                     FFLSTNTTRSTLSWSDANEYGVKSGERYNYNAETDSWEANDNFISTDDGFPLSFKTGL
                     FYSGQVNEKLKITANYAYSDFRKKTYNTNKTQYFLSDTSYSNNSESTANLRFMGHEAN
                     LLFSYKIDSTQTIKIHPQFTFSNRNNLQNDISGYYNSQGASTRNSTNISNSNSQATNI
                     KALIDYTKKFKKDKREFRIFNNFIYDVSGIKSNQQYSDYFTQTNLYENEIDQQYKSDR
                     SIISNILSASYTEPLTKKWRIVFNYELFTTTNQQIKNNYNNNNGNYDQLDSITSGNFK
                     SIKLQNKLGASLHYDFKKHSVKIGFTARNVLIDNQNLFVHSVLKQNNTTVLPSFMYKF
                     KISKNSEFRINANANSSLPDINFLQPIANNANPNSIFIGNTNLKPSYTASSNISYNIY
                     NPMSGGYLWTGVNSSYGWNQLISTTQFDAIGRRITSYLNGNTLNYLSGYLNGGIPLIK
                     QVLSLEPEISYVYNNRINYVDNLKNTLVAHTIEPSIGISAYLDVVDISFGFDYAYTSN
                     "
BASE COUNT          645 a          335 c          381 g          755 t
ORIGIN      
        1 ttgttagatg tgtaggcata atcaaatcca aaactaatgt caactacatc taaataggcg
       61 ctaattccta ttgaaggttc aatggtatgg gctacaagtg tatttttgag attatcgaca
      121 taattaattc gattgttgta aacgtagcta atttcgggct ccaacgataa aacttgctta
      181 attaatggaa ttccgccatt tagataacca ctcaggtaat tcaacgtatt accatttaga
      241 taactggtta ttcttcgacc gatggcatca aattgagtgg tagaaattaa ttgattccaa
      301 ccataagatg aattaactcc tgtccataaa tatcctcccg acataggatt ataaatgttg
      361 taactaatat tagaagaagc tgtgtaggaa ggttttaaat tggtgtttcc tataaaaatg
      421 ctgttgggat tggcattatt tgctataggt tgtaaaaaat taatatcagg caacgaagaa
      481 ttggcatttg catttattct aaactcgctg tttttgctga ttttaaactt atacataaaa
      541 cttggtaaaa cggtagtgtt attctgcttt aagacggaat gaacaaacaa attttgattg
      601 tctatcaaca cgtttcttgc tgtaaatccg atttttacgg aatgtttttt aaagtcataa
      661 tgcaagctcg cacccaattt attttgcaat ttgatacttt taaaattacc agatgttatt
      721 gaatctaatt gatcgtaatt gccattgtta ttgttgtaat tgttttttat ttgttgattg
      781 gtagtggtaa acaattcata attaaataca atacgccatt ttttagtcaa cggttcggtg
      841 taggaagctg ataggatgtt agaaattata gaacgatcgg atttgtattg ttggtctatt
      901 tcattctcat acaaattggt ttgcgtaaaa tagtcactgt attgttgatt ggatttaatg
      961 cccgatacat cgtaaataaa gttgttgaaa atgcggaact cacgcttatc cttcttaaat
     1021 tttttggtgt aatctatcaa cgctttaatg ttggttgctt gactgtttga attactaatg
     1081 tttgtagaat ttcgggtaga tgctccttgg ctgttataat agcctgatat atcattttgc
     1141 aggttattgc ggttagaaaa agtaaattgt gggtgaattt taatggtttg agtagaatct
     1201 attttatagc taaaaagtag attcgcttcg tgacccataa aacgaagatt ggcagtgctt
     1261 tccgaattat ttgaataaga agtgtcactt aaaaaatact gtgttttatt ggtgttgtat
     1321 gttttttttc taaaatcact gtaggcataa ttggctgtta tttttagttt ttcgtttact
     1381 tgcccagaat aaaacaaacc agttttaaag ctaagaggaa agccatcgtc ggtggaaatg
     1441 aaattatcat ttgcttccca actatcggtt tctgcattgt aattataacg ttcaccactt
     1501 ttaactccgt attcatttgc atcactccaa ctcaaggtgg aacgtgttgt attggtggaa
     1561 aggaaaaaag cagcaatttt atggtccttg ctaaatttat tgaacaatgc ttgtccctca
     1621 aaatagttca tgaaatcagt accaaatgcc actttgccaa aatatccttt tttagcagca
     1681 tctttgagtt ctaaattcaa aactttaact ttttcatctg tagaagaagc gtcgggtagg
     1741 tgggtttcat acacttgcac acgttccaca ctgttagccg ctaaattttt ggttgccaag
     1801 gtagcatcag agccaaaaaa ctcatcgccg tccacataaa tcctcgaaat gtctttacct
     1861 tgacttttga tggtgccatt tttttcaact gtaattccag gtagcttttt aagcaaatct
     1921 tccacaacag cattggggcg ggttttgaat gaatcggcaa tatataccaa cgtatcacct
     1981 ttaaaatgaa cagggtcttt ataggcaaaa atggttactt catcaatttt ctttccgcgc
     2041 tcaaacaaaa ccatattgtt gagcttaaat tcggaattgt tttcattggc aaaaaagtaa
     2101 aaatcacgtg tttcat
//
LOCUS       DAOQVQ010000438         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66442_length_2111_cov_3.550097, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000438 DAOQVQ010000000
VERSION     DAOQVQ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66442_length_2111_cov_3.550097"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..>2111
                     /locus_tag="PKN22_10630"
     CDS             286..>2111
                     /locus_tag="PKN22_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNS43204.1"
                     /translation="MIIGALTLLTVFFGYEAAINIKLDNEYGFILPKDSEAEVDYQKF
                     TSQFGDEGGSFVIAIQTDSLYTEDKFLKWKQLGDSILTFEGIDGVISEATLFNVENNT
                     SKQRFDVRKIFSDTRYIEKPITQIKDEVKRVPFYNGILYNDSTNVSLMVINVQDDYLT
                     YQKKANVVLKIKELAQSYEQDFGKVRFSGLSYIRVILSKRIVSEMYLFIALAILATSL
                     ITYLFFRSHRVVVICNIVIGIGVIWSLGIIALLGYHISVLMALIPPLMIVIGMPNCVF
                     LMTHFHQEIKQHRNKIKALTKVIKEVGNATFITNFITAIGFFSLMYTNSDRLVEFGLV
                     AGVSILVLFLLSICILPIISTLTKAPSDKHLRHLDRGLAVQFVRATVYIVSKRRVWVY
                     VATIVALVFSGIGMLKVKSTGNLTGDISPNDPIVKDIQFLENNLGGSIPFEVLINYKN
                     KENLYNPELLQKVDAVQQLLGQDTAFSKAISLVELIKAVNMSYHGNDSTKYRLFDNQN
                     KVYLQKYIENFDAASISSTLQLKELLDTTNTTIRVRTQIKDYGSYEMSEKAQQIKIEI
                     DKKINPFKDKQEHFYVNVVSGELNYIDSILLKNPTIAQKLAK"
BASE COUNT          694 a          343 c          385 g          689 t
ORIGIN      
        1 tgagaaagca caaactgaac aacaacaagg tacacaaggt ggacaaaatg gtgcaactcc
       61 acaaggaggg caacaacaac agccaaagta actaaagtaa cgatatttta tagttagaag
      121 ggttgtacaa taggtgtgca accctttttt aattttcgtt catgcttaaa aagatagatg
      181 ttaatttttc aaatattttt ttatttttac aacactcttt aattaaacag tcggtaaata
      241 ttaatgtggt attactttgc atacattatt ttaaggttta agttcttgat aataggagcg
      301 ttaactctat tgactgtttt ctttggttac gaagctgcta ttaacatcaa attagataat
      361 gaatatgggt tcattttacc caaagattca gaagcagaag tagattatca aaaatttaca
      421 tcacaatttg gtgatgaagg cggatctttt gtgattgcca ttcaaaccga ctctctttat
      481 accgaagata aatttttaaa atggaaacag ttgggcgact ctattcttac atttgaagga
      541 atagacggtg ttatatccga ggcaacgtta ttcaacgttg aaaataatac cagcaaacaa
      601 cgttttgatg tgcgtaagat tttttcagac acccgttaca ttgaaaaacc aattactcaa
      661 attaaagatg aggtaaaacg tgttcctttt tataacggga tattgtataa tgacagtacc
      721 aatgtttcgt tgatggtcat caatgtgcaa gacgattatt tgacgtatca gaaaaaggca
      781 aatgtagtgc tgaaaatcaa agaattggca cagtcttatg agcaggattt tggtaaagtt
      841 cgcttctctg gactttctta tattcgtgtg atattgagca agcgcatagt ttctgaaatg
      901 tacttgttta ttgctctcgc aatacttgca acgtcgttaa ttacttattt gttcttccgc
      961 tctcaccgag tggtagtaat ttgcaacatt gttattggta tcggcgtaat ttggtcgttg
     1021 ggaattattg cattgttggg ttatcacatt tcggttttaa tggcgcttat tccgccgcta
     1081 atgattgtca taggtatgcc taattgtgtg tttttaatga cgcattttca ccaagaaata
     1141 aaacaacatc gtaataaaat aaaagcatta accaaagtta tcaaagaagt aggaaacgct
     1201 acgtttatta ccaatttcat tactgcaatc ggtttctttt cgctcatgta caccaattcc
     1261 gatcgtttgg tggagtttgg tttggttgca ggtgtttcta ttttggtttt attccttctg
     1321 tctatttgta ttttaccgat tatttcaacc ctaacaaaag ctccttcaga caaacatttg
     1381 cgccatttgg atagaggctt ggcggtgcaa tttgtaagag ctacagttta cattgtaagt
     1441 aaacgtcgag tttgggtgta tgtggctaca atcgttgcac ttgtatttag cggaattggg
     1501 atgctaaaag taaaatcaac tgggaatcta acaggtgata tttctccgaa cgacccaatt
     1561 gtaaaagata ttcagttttt agaaaacaat ttaggtggtt ccattccatt tgaagtactc
     1621 atcaattata aaaacaaaga aaatctttac aatcctgaac tactgcaaaa agtagatgct
     1681 gttcaacaat tactgggtca agatacagcc ttttctaaag ctatttcctt ggttgaactc
     1741 atcaaagcag taaacatgtc ctatcatggc aatgattcaa caaaataccg tttgtttgac
     1801 aaccaaaaca aagtgtattt gcagaaatat atagaaaact ttgatgcagc aagtattagt
     1861 tcaacattgc agttaaaaga attgttggat actaccaata ccaccattcg tgttcgcacg
     1921 caaataaaag attatggttc gtatgaaatg agcgaaaaag cgcaacagat taaaattgaa
     1981 attgataaaa aaatcaatcc gtttaaagac aaacaagagc atttttatgt caatgtcgtt
     2041 tctggagagc tcaattatat agattccatc ttgctaaaaa atccaacgat tgctcaaaag
     2101 ttagccaaac a
//
LOCUS       DAOQVQ010000439         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66501_length_2110_cov_3.569830, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000439 DAOQVQ010000000
VERSION     DAOQVQ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66501_length_2110_cov_3.569830"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1465
                     /locus_tag="PKN22_10635"
     CDS             266..1465
                     /locus_tag="PKN22_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS43205.1"
                     /translation="MRTDLYTHPDYYNMDDLLSDEQKLVRDAARAWVKKEVSPIIDDY
                     YERATFPKQLIKGLGEIGAFGPYIPEQYGGPGLDQISYGLIMQELERCDSGLRSTASV
                     QSSLVMYPIWKYGSEEQRMKYLPKLATGEFMGCFGLTEPDHGSNPGGMITNFKDAGDH
                     VILNGAKMWISNAPFADIAVVWAKNEEGRIHGLIVERGMEGFTTPETHGKLSLRASAT
                     GELVFDNVKVPKENILPGRTGLGAPLSCLDSARFGIAWGALGAAMDCYDQALRYSKER
                     VQFGVPIGAFQLTQKKLAEMITEITKAQLLLLRLGQLREAGKATSAQISMAKRNSCEI
                     ALNIAREARQIHGGMGITSEYSIMRHMANLESVVTYEGTHDIHLLITGMDVTGIPAFT
                     REMPDLK"
     gene            1601..>2110
                     /locus_tag="PKN22_10640"
     CDS             1601..>2110
                     /locus_tag="PKN22_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNS43206.1"
                     /translation="MTQNLVIYNSLTGNKEVFKPLHEGRVGMYVCGPTLYSEPHMGNM
                     RTFINFDLVYRYFLKLGYQVKYVRNITDAGHITNSAGEDVDSIGKAAKLAQVQSLEIV
                     YKFNRKFIELQRIYNMLPPSIEPTATQHIQEQIEIIEQIIDNGYAYVTNGSVYFDVRK
                     YSETYNYGIL"
BASE COUNT          725 a          360 c          406 g          619 t
ORIGIN      
        1 aataatatat ggatctaatc gtttagccaa ggccaacaat aaaaaactta aaaacaaaac
       61 aaaccctaat atccattgat ttgcgcccgc aatagcgtcc aaatgtgtag gaacttcaac
      121 atccttccct cctactattg aaaaaagcgg tatgaatcct aaaaaattga acatatttat
      181 gaatattaag atgtttcttt aacaaaaata actttgagag aacaaaatta tactattttt
      241 gcctatcatt aatagatttt ttaaaatgag aacagattta tatacacatc ccgactatta
      301 taacatggat gatttgcttt cagatgagca aaaattagta agagatgctg ctagagcatg
      361 ggtaaaaaaa gaggtttctc caattatcga tgattattat gaaagagcaa ctttccccaa
      421 acaattaatt aaaggattgg gtgaaattgg tgcttttggt ccttatattc cagaacaata
      481 cggtggtcca gggttggatc aaatttcgta tggtttgatt atgcaagaat tggaaagatg
      541 tgattctggt cttcgttcaa ctgcatctgt tcaatcttca ttggtgatgt atccaatctg
      601 gaaatacggt tcagaagaac aacgcatgaa atatttacca aaattagcaa ctggtgaatt
      661 tatgggatgt tttggtttaa cagaacctga ccatggatcc aatccaggtg gtatgattac
      721 caacttcaaa gatgcaggtg atcatgtaat tttgaacggt gcaaaaatgt ggatttctaa
      781 cgctccattt gctgatatcg cagtggtatg ggcaaaaaac gaagaaggaa gaattcacgg
      841 attaattgtt gagcgtggaa tggaaggatt tacaactcca gaaactcacg gaaaactttc
      901 attgcgtgca tctgcaactg gtgaattggt ttttgacaat gttaaagttc caaaagaaaa
      961 tattttacca ggaagaacag gtttaggcgc tccattaagt tgcttagact ctgctcgttt
     1021 tggtattgca tggggtgcgc ttggcgctgc tatggattgc tacgatcaag cgttgcgata
     1081 ttctaaagaa cgtgtgcaat ttggtgttcc aattggtgct ttccaattaa ctcaaaagaa
     1141 attggctgaa atgattaccg aaatcacgaa agctcaatta ttgttattgc gtttaggaca
     1201 attaagagaa gctggaaaag caacttctgc acaaatttca atggcaaaac gcaacagctg
     1261 tgaaattgca ttgaacattg cacgcgaagc acgtcaaatt cacggaggaa tgggaatcac
     1321 aagtgagtat tcaatcatgc gccacatggc taacttagag tcagtagtta cgtatgaagg
     1381 aactcacgac attcacctac taatcacagg aatggatgta actggtatcc ctgcattcac
     1441 aagagaaatg cctgatttga aataatcaga caccatacat ttaaaaaaga gaatttgagc
     1501 aatcaagttc tcttttttta tagcatcctg caaaggacca aaaatttcaa tagaatttct
     1561 taagtttgca tattaatcgt caatcaaaaa aacgtttcaa atgacacaaa atttggtaat
     1621 ctacaacagt ttaacaggta ataaagaagt ttttaaacca cttcacgaag gtagagttgg
     1681 tatgtatgtt tgtggtccaa cactctacag tgagccacac atgggcaaca tgcgcacatt
     1741 tatcaacttt gatttggttt accgttattt cctcaaattg ggttatcaag taaaatatgt
     1801 gcgcaacatc actgatgccg gacacattac taacagcgct ggtgaagatg tggatagcat
     1861 cggaaaagca gctaaattgg cacaagtgca atcgttggaa attgtatata aattcaaccg
     1921 aaaattcatt gaattacagc gtatttacaa catgttgccg ccaagcatag aaccaacagc
     1981 tacacaacac attcaagaac aaattgaaat cattgagcaa attattgaca atggttatgc
     2041 ttatgtaacc aatggttctg tttattttga tgtgcgcaag tattctgaaa catacaatta
     2101 tggtatttta
//
LOCUS       DAOQVQ010000440         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66783_length_2105_cov_1.960976, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000440 DAOQVQ010000000
VERSION     DAOQVQ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66783_length_2105_cov_1.960976"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKN22_10645"
     CDS             complement(<1..555)
                     /locus_tag="PKN22_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNS43207.1"
                     /translation="MFTPSDLEQLKHKNISVATVEQQLTSFVQGFPYLNIKRVAAIGE
                     GLLQLTESELEVAIETWEQYLQGTHQIEKFVPASGAASRMFKDLFEFLNGENDVPTTP
                     FEKHFFEHISRFAFFADLNEACLRNEQLTIDELIKAGRYKDVVANLLEKKGLDYGTLP
                     KGLLKFHRYPHENRTPFLEHLVEGA"
     gene            complement(669..>1697)
                     /locus_tag="PKN22_10650"
     CDS             complement(669..>1697)
                     /locus_tag="PKN22_10650"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNS43208.1"
                     /translation="NQSGMVGQLHIVDFLTKIGTRIACVFAPEHGFRGYRDAGAKIYT
                     TTDKQTGLPIRSLFNGDVSKPTAEALADIDIVVFDLQDVGVRYYSYLTTLFRVMEACA
                     ENNKPLLILDRPNPNGFYVDGPILDMRYKSMWGILPIPIIHGMTLGELASMINGEHWL
                     ANNLQCNLTVITCKNYTHQTKYVVPVPPSPNLPTMRSVYLYPSLVYFEATPISIGRGT
                     EFPFQVYGHPNMLGCTFSFNPESVRGAKNPPLRNFQCQGRDLRTLPSDSALFAEGIDL
                     RYLIDAYWNMHLGSFFFNSFFETLIGVDYVKPMIINGRSAAEIERRWLPDVEKFKQQR
                     KPYLLYPE"
     assembly_gap    1698..1707
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1830..>2105)
                     /locus_tag="PKN22_10655"
     CDS             complement(1830..>2105)
                     /locus_tag="PKN22_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43209.1"
                     /translation="PIRLVNQLLKLLVEVHLLSEIYTDNNQEKSYQPAMDIHQITIQL
                     VFDKISTFGSENFKVNPKKEFDGMWEQLEQFRLKTVNHSSDLLIKDL"
BASE COUNT          625 a          388 c          410 g          672 t
ORIGIN      
        1 agcgccttct acgagatgtt ccaaaaatgg tgttctgttt tcgtgtggat atctgtgaaa
       61 tttgagtaac cctttaggca gcgtgccata gtcgagccct tttttctcca acaaattagc
      121 taccacatct ttatatctac cagcttttat aagttcatct atggttaatt gttcattgcg
      181 caagcaggct tcgtttaaat cagcaaaaaa tgcaaatcgc gaaatgtgct caaaaaaatg
      241 tttttcgaaa ggtgttgttg gaacgtcgtt ttcaccattc aaaaattcaa ataaatcttt
      301 gaacatgcga ctagccgcgc ctgaagctgg tacaaatttt tctatttggt gggttccttg
      361 caaatattgt tcccacgttt caatagccac ctctagttcc gattcggtca gttgaagaag
      421 cccttcgcca atggcagcca cgcgttttat atttaaatac ggaaatcctt gtacaaaact
      481 ggttagttgt tgttcaactg ttgctactga aatgtttttg tgttttaatt gttctaaatc
      541 ggatggtgta aacatagcta tagtgtaaat aaggttagtt aggtattagc atactataca
      601 aagatagcaa aatgcccata aaaaagcaaa tatacaaata tgttgtttag cagatagcgt
      661 gttgcgtttt attcaggata gagcaaatat ggtttgcgtt gttgtttgaa tttctcaaca
      721 tcaggtaacc agcggcgttc aatttctgct gctgagcgtc catttataat cataggtttt
      781 acataatcta ctccgattaa tgtttcaaaa aatgagttga agaaaaaaga acctaagtgc
      841 atattccaat aggcatctat caaataacgc aaatcaatgc cttctgcaaa caaggcacta
      901 tcgctaggta atgttcgtaa atctctacct tgacattgga aattgcgtaa cggtggattt
      961 ttggcaccac gtaccgattc ggggttaaac gaaaatgtac agcccaacat gttaggatgt
     1021 ccgtatactt ggaatggaaa ttctgtacct cgaccaatac tgataggtgt tgcttcaaaa
     1081 tacacaagtg atggatagag ataaaccgat cgcatggttg gtaagtttgg cgatggagga
     1141 acgggaacaa cgtattttgt ttgatgtgtg taattcttac aggtaatgac agttaagtta
     1201 cattgcaaat tatttgctaa ccaatgttcg ccattaatca tacttgccag ttcgcccaag
     1261 gtcatgccat gtataatcgg aataggtaaa atgccccaca ttgatttgta acgcatatcc
     1321 aaaataggtc cgtctacata aaatccgttt gggtttggtc tgtcgagaat tagtagcggt
     1381 ttattatttt cagcacacgc ttccattacc cgaaataacg tggttaaata ggagtaataa
     1441 cgaacgccta catcttgtaa atcaaatacg acgatatcga tatcggcgag tgcttctgct
     1501 gttggttttg atacatcacc attaaaaagg gaacgaatag gtaaacctgt ttgcttgtcg
     1561 gtggtggtgt aaattttggc gcccgcatcg cggtagccgc gaaaaccatg ttcgggagca
     1621 aaaacacagg caatgcgtgt acctatttta gtcagaaaat cgacgatatg aagttgccct
     1681 accataccgc tttgattnnn nnnnnnncag cgataaatag cgttctaact gatcagcacc
     1741 tactacaaca ggttcgcttg cccaaagttt tagtgtacaa actaaaccac acacaatcca
     1801 aataagtttt ttcatgggta cctccttttt taaaggtctt ttatgagtaa atcgcttgag
     1861 tgattaactg tttttagacg gaattgttct aattgctccc acattccatc aaattccttt
     1921 ttagggttta ctttaaaatt ttctgatccg aacgtactta ttttatcaaa tactaattga
     1981 atagtaattt ggtgtatatc catagctggt tgatatgatt tctcttgatt gttgtcagta
     2041 taaatttcag ataagagatg aacttctacc aataatttta ggagttgatt caccaatcgt
     2101 atcgg
//
LOCUS       DAOQVQ010000441         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66854_length_2103_cov_3.479980, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000441 DAOQVQ010000000
VERSION     DAOQVQ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66854_length_2103_cov_3.479980"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PKN22_10660"
     CDS             <1..621
                     /locus_tag="PKN22_10660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS43210.1"
                     /translation="YSSSACIYPEENQLQPDSPNCEESSAYPANPDSEYGWEKLFSER
                     LYLSYKRNYGLNVKIARFHNIYGPESTWNGGREKAPAAICRKVAQAKQNEAIEIWGDG
                     LQTRSFLYIDDCLDAVLKLMRSNFTGPYNIGSEEMISMLDFTKLVLKISNKNNTIQTI
                     DGPQGVRGRCSHNALISRDIQWQPNYNLQQGISKLYAWIDSQVKNL"
     gene            631..>2103
                     /locus_tag="PKN22_10665"
     CDS             631..>2103
                     /locus_tag="PKN22_10665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNS43211.1"
                     /translation="MGKKDTKKTMANTVSVQPQNPLKKYYVIFGLLAFFLFANTLGNG
                     YNMDDSMVTNNHKLTSKGLSAIGEIFTSPYYSDDAGYAYGYRPMVHLSFALEQELFGQ
                     KPGAGHFINVLLFVISVILFFKLLVKWVGEKNILFAAVATLLFTVHPIHTEVVASLKN
                     RDELLAFLFVIWAGVCAFKYLEKEKISSLIAVFLLFSLAMLSKKSVYPMAIILPVSMV
                     LLHTVPLKKNSAIALAMVLPAAIIGSDLLVERMFIMFALALAAIGSATYVKYQFLTPN
                     NSDTTKKQAWILPVILLFIVVMASFYFSSLYLSVLAIPLFVWLLYLHFNTGLLSIILY
                     STALFFLFNRTAFIALPIMLALGYGVYLWKNKQSYAYWLIGALVLIIAMQFLQFNPLF
                     LLNFLAVVVFIGLLISNILYGVGYLLLVVVGYFYFDSLYNPFLISLISILILWFIYQK
                     TNSKTWIVLIPVVFFSIVYSHNAYQQYLYDSANQPTTQTVQ"
BASE COUNT          610 a          372 c          391 g          730 t
ORIGIN      
        1 tattcttcat cggcgtgcat ttatcccgaa gaaaatcaat tgcaacccga ttctcccaat
       61 tgcgaggaaa gttcggccta tccagccaat cccgatagcg aatacggttg ggaaaaattg
      121 tttagcgaac gcctttattt gtcctacaaa agaaattatg gattaaacgt aaaaattgct
      181 cgttttcata atatttatgg tcctgaatca acgtggaatg gtggacgtga aaaagcacct
      241 gctgccattt gcagaaaagt ggcacaagca aaacaaaatg aagctattga aatttggggt
      301 gatggcttgc aaacacgtag ttttttatat attgacgact gtttggatgc tgttttaaaa
      361 ttgatgcgct ccaattttac tggtccctac aacattggct ccgaagaaat gatttccatg
      421 ctcgatttta ctaaattggt attgaaaatc agtaataaaa acaataccat tcaaaccatt
      481 gatggtccgc aaggagtgcg cggaagatgt tcacacaacg cactcatttc tagagatatt
      541 caatggcaac caaactacaa tttacaacaa gggatttcaa aattatatgc ttggattgat
      601 agccaagtta agaacttgta aaactacttt atgggaaaaa aagacactaa aaagacaatg
      661 gcgaatacag taagtgttca acctcaaaat ccactcaaaa aatactatgt aatttttggt
      721 ttattagcat tctttctgtt tgccaacacg cttggaaatg gctataacat ggacgattcc
      781 atggttacca ataaccataa attaacctcc aaaggattga gtgccattgg tgaaatcttc
      841 acctcaccct attatagcga tgatgccggt tatgcttatg gttaccgacc aatggtgcat
      901 ttgtcatttg cattagagca ggaacttttt ggacaaaaac caggagccgg acatttcatt
      961 aatgtgttgt tgtttgtgat ttcggttatt ttgtttttta aattattggt gaaatgggtg
     1021 ggtgaaaaaa acattctttt tgctgccgtt gcaacgcttt tatttacagt tcatcccatt
     1081 cacacagagg tagttgcaag tttaaaaaac agagacgagt tactggcgtt tttatttgtc
     1141 atttgggcag gtgtatgtgc ctttaaatat ttggaaaaag aaaaaattag ctcactcata
     1201 gcagtattct tgctgttttc tttggcgatg ctttctaaaa aaagcgttta tccgatggct
     1261 ataattttac ccgtttcaat ggtattgttg cacacggttc cgcttaaaaa aaatagcgca
     1321 attgcacttg caatggtgtt gccagcagct attataggtt cggatttact ggtggagcgt
     1381 atgtttatta tgtttgcctt ggcacttgct gcaattggaa gtgcaactta tgtaaaatac
     1441 caattcttaa ctcctaacaa cagcgataca accaaaaagc aagcgtggat acttcctgtc
     1501 attttgcttt ttattgtagt gatggcttcg ttttattttt cgtccctgta tctcagtgtt
     1561 ttagcaatac cactttttgt atggttgctg tatcttcatt tcaataccgg tttgctttcc
     1621 attattttgt attctacagc actttttttc ttattcaata gaacagcttt catagctttg
     1681 cccatcatgt tagctttagg gtatggggtc tatttatgga aaaataaaca atcctatgcg
     1741 tattggttaa ttggagcttt ggtattaata attgcaatgc aatttcttca attcaatccg
     1801 ttgtttttat tgaatttctt ggcggtagtg gtatttattg ggttgttaat ttccaacatt
     1861 ttgtatggag tgggctatct cttgttggtg gtagtaggat atttttattt cgattctctt
     1921 tacaatccgt ttttaatttc gctcatcagt attctcattc tttggttcat ttatcaaaaa
     1981 acaaactcca aaacatggat tgttttaatt ccagtcgtgt tttttagtat tgtttatagt
     2041 cacaatgcct atcaacaata tctctatgat agcgccaatc aacctactac tcaaacagtt
     2101 caa
//
LOCUS       DAOQVQ010000442         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_66861_length_2103_cov_3.177734, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000442 DAOQVQ010000000
VERSION     DAOQVQ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66861_length_2103_cov_3.177734"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..750)
                     /locus_tag="PKN22_10670"
                     /pseudo
     CDS             complement(32..750)
                     /locus_tag="PKN22_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
     gene            complement(822..>2103)
                     /gene="ligA"
                     /locus_tag="PKN22_10675"
     CDS             complement(822..>2103)
                     /gene="ligA"
                     /locus_tag="PKN22_10675"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNS43212.1"
                     /translation="QWGLKVPSSQQRYIQKVNSIEGIMDFIHYWDKKRDELPFAIDGI
                     VIKVNNYAQQEELGFTAKSPRWATAFKFKAERVETQLLSIDYQVGRTGVVTPVANLAP
                     VHLGGTTVKRASLHNADQLEKLDLHANDFVFVEKGGEIIPKIVGVNNDKRTPSAQKIQ
                     FIETCPECSTPLVRNEGEAQHFCPNDSFCPPQMTGKIEHFIGRKAMNIDGLGTETIQL
                     LFDKGLIKNAADLYALTFDELLKLDRMAEKSANNLLKGLQESKKVPFERVLFALGIRY
                     VGETVAKKLAKHFKNIDAIQQATLLELTLVEEIGEVIAQSVNNYFEQPQHRALIERFK
                     AIGLQFEIEEAELSSTLFVGKSFVVSGVFNAFSRDELKALIEANGGKNVSSISSKTDY
                     VVAGDNMGPAKLQKATDLNIPILSEDDFIKMLNQ"
BASE COUNT          615 a          419 c          341 g          728 t
ORIGIN      
        1 aataattgga tttttttcaa aaatcttcct gtcaaaagtc ttttaataaa aatttataac
       61 gttgtatggt attctctaaa ccaaaataca atgcatctga aatcaatgca tgaccgatgg
      121 atacttccgc taaaaagggg atattttgtt tgaagaattt caaattttct aaacttaaat
      181 cgtgcccagc atttaattcc aaccccaaag atttagcaaa attagccgct tccacataag
      241 gagcaattgc tttttctttg ttttgcgaat atacttcagc ataaggacca gtatataatt
      301 ctatacgatc tacaccaatt ttagctgcat attcaatatt ttgcaaattg gtgtcaacaa
      361 aattgagctt cgcacgttga tgctttttaa atgtgccact aaatctttga gtaaatcata
      421 atttgttttg gtatcccaac cagcattgga agttaaaact cctggaggat cgggtaccaa
      481 agttgcttgt gctggcatag tttcttcaac caattgcata taccttgcat cgggataacc
      541 ttcaatattg aattctgttt taactacttt atacaaatca taaacatctt gtgtggtgat
      601 atggcgttcg tctgggcgtg gatgcaccgt aattccatca gcaccaaatc gttcacaatc
      661 tattgcaact tgcacaacat taggcgtgtt tcctcctctg gcatttcgga tagttgcaat
      721 tttattgatg tttacactta attttgtcat ttcttatttt ttactccaaa agtactattt
      781 ttccatcaca accctttata ttattgcaat tagttgtgtt tttattgatt cagcatttta
      841 ataaaatcgt cttcgcttaa aatcggaatg tttaaatcgg ttgccttttg caatttggct
      901 ggccccatgt tatctcctgc aaccacataa tctgttttgc tggaaataga cgatacattt
      961 ttccctccat ttgcttcaat caaggctttt aattcatcgc gagaaaatgc attgaaaaca
     1021 cccgaaacta caaacgattt tccaacaaaa agagtagagg agagctcagc ttcttcgatt
     1081 tcaaattgca acccaattgc tttaaaacgc tcaatcaacg cacgatgctg gggttgctca
     1141 aaataattgt ttaccgattg cgcaattact tcgccaattt cttctaccaa ggttaactcc
     1201 aacaaagttg cttgctgaat ggcatcaatg tttttaaaat gtttagctaa ttttttagcc
     1261 acagtttcgc caacataacg aattcctaaa gcaaaaagta ctcgttcaaa cggaactttt
     1321 ttagattctt ggagtccttt caataaatta ttggcacttt tttcggccat acggtccaat
     1381 ttcaacaatt catcaaaagt aagtgcgtat aaatcggctg cgtttttaat caatcctttg
     1441 tcaaacaata attgaatggt ttctgttccc aaaccatcga tgttcattgc ttttctgcca
     1501 ataaaatgct caattttacc agtcatttgt ggtggacaaa aactatcgtt tggacaaaaa
     1561 tgctgtgctt cgccctcgtt tctaactaaa ggagttgaac attctgggca ggtttcaata
     1621 aattgaattt tttgagcaga tggagttctt ttatcgttat ttaccccaac aatttttggg
     1681 ataatttctc cacctttttc cacaaaaaca aaatcgtttg catgcaaatc caatttttcc
     1741 aattgatcgg cattgtgcaa agaggcacgt tttacggttg tgccacccaa atgaacgggt
     1801 gcaagattgg caacaggagt tacaacacct gttcttccaa cctgataatc aatggataat
     1861 aattgcgttt caactcgttc tgctttaaat ttaaaggccg ttgcccagcg tggagatttg
     1921 gctgtaaaac ctaattcttc ttgttgcgcg taattgttga cttttataac aattccgtct
     1981 attgcaaaag gcaattcatc tcgtttttta tcccaataat gaatgaagtc cataatccct
     2041 tcaatggaat tgactttttg gatgtagcgt tgttgcgacg atggtacttt caatccccat
     2101 tgt
//
LOCUS       DAOQVQ010000443         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_67772_length_2085_cov_3.144828, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000443 DAOQVQ010000000
VERSION     DAOQVQ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67772_length_2085_cov_3.144828"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PKN22_10680"
     CDS             <1..762
                     /locus_tag="PKN22_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS43213.1"
                     /translation="KPSECGTTNGSITITGTNLKELFAVSENGDTTSVIGNILTGLGA
                     GNYTVFYTNASGCQSADTLITIGTVNNTIANFTATPQSGSTPLEVAITNTSQNAQQFQ
                     WFVNGELQPNPFSSFVADTSGSYQITLVAWKNDLSCADTAQLTVIAYDSLIVGIPNVF
                     SPNNDGVNDVFSITVNQPVKCELVIVNRWGEKMFSYSGNLTVGMNDLWSADATVSDGV
                     YFYSVKMETSPPPLSPSKGGNPHQLSREGFVTVVR"
     gene            complement(786..1091)
                     /locus_tag="PKN22_10685"
     CDS             complement(786..1091)
                     /locus_tag="PKN22_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNS43214.1"
                     /translation="MIRVVKLTFQEDKIADFLAFFDTINHIVNEFPGCNGMQLLQDRQ
                     HPFIVFTYSDWKDEAALENYRVSPDFQRIWSTIKPWFSEKAQAWSVDVYFNGFEKKS"
     gene            complement(1088..1957)
                     /locus_tag="PKN22_10690"
     CDS             complement(1088..1957)
                     /locus_tag="PKN22_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNS43215.1"
                     /translation="MQIITLTSDMELRDYYVSSLKVSILKQCKNPIHLLDVSHFITPF
                     DVAEAGFVLGQAYHDYPDGTIHLMCLEAEPVVNFGNSEGSFPSVMVLNNQYFIGVDNG
                     FFGVLERFGTVNAFYRIEEALSSPKNYTFPAKNLLAPYACRLANGEKIEDFAVPHSTY
                     KKALSVVPNIEKDLILGSVIYFDSMGNAVTNIDKELFEKVGKDAPFIIKFRDKTIYEI
                     DKISTSYNDVPHGEKMAIFNERGLLEIAINKGANRTTIGAQKLLGISKNDIVRVDFSP
                     RGSKDSLNDLFKL"
BASE COUNT          684 a          374 c          367 g          660 t
ORIGIN      
        1 aaaccatcag aatgtggtac tacgaatggt tctattacta taacaggaac aaacttaaaa
       61 gaactatttg ccgtatccga aaatggtgat acaacaagtg ttataggaaa tattcttaca
      121 gggcttggtg ctggtaatta cacagttttt tataccaatg catctggttg tcaaagtgca
      181 gacacactca taacaattgg tacagtcaac aataccatag ccaattttac ggcaactcca
      241 caaagtggaa gcactccttt ggaagttgct attaccaata catcgcagaa tgcacagcaa
      301 tttcaatggt ttgtaaacgg tgagttgcag ccaaatcctt ttagttcttt tgtggcagac
      361 acatcgggta gttatcaaat cacattggtt gcttggaaaa acgatctttc atgtgcagac
      421 accgctcaat taaccgttat tgcttatgac agtttaattg ttggtattcc caatgtgttt
      481 tcacccaaca acgatggtgt aaatgatgtt tttagcatca ctgtcaacca acctgtgaaa
      541 tgtgaattgg tcattgttaa ccgttgggga gaaaaaatgt tttcctactc gggaaattta
      601 accgtaggaa tgaacgattt gtggtcggca gatgctaccg tttctgatgg cgtgtatttt
      661 tatagtgtga aaatggaaac gagtcctccc cctttatccc cctccaaagg gggaaacccg
      721 catcaattat caagagaagg gtttgtgacg gtggtgaggt agggaagatg tgattttatt
      781 gaatattacg atttcttttc aaatccgtta aagtaaacat ccacactcca tgcttgtgcc
      841 ttttctgaaa accaaggttt aatggttgac caaatacgtt gaaaatcagg tgaaacacga
      901 tagttttcca aagcagcttc gtctttccaa tcactataag tgaaaacaat aaacggatgt
      961 tgtctatctt gtaacaattg cattccattg caacctggaa attcatttac aatgtgattg
     1021 atggtatcaa aaaatgctaa aaaatcagca attttatctt cttgaaaagt aagttttact
     1081 actcgaatca tagcttaaac aaatcattga gagaatcttt tgagcctctt ggagaaaaat
     1141 caacacgaac tatatcgttt tttgaaatcc ccaataattt ttgtgctcca attgtagtgc
     1201 ggtttgctcc cttattaatt gcaatttcca ataagccacg ttcattgaaa atagccattt
     1261 tctcaccatg aggcacatcg ttgtaggaag tactaatttt atcaatttcg tagattgttt
     1321 tatctctaaa cttaatgatg aatggcgcat ctttacccac tttttcaaat aattctttat
     1381 caatattggt aactgcattt cccattgaat caaaataaat aacagaaccc aaaataaggt
     1441 ctttttcaat atttggcaca acagaaagtg cttttttgta ggtagaatga ggcacagcaa
     1501 aatcttcaat tttttcgcca ttggctaatc tacacgcata gggtgctaac aaatttttgg
     1561 ctgggaaagt gtaattttta ggtgatgata atgcttcttc aattctgtaa aaagcattca
     1621 ctgttccaaa acgttctaaa acaccaaaaa aaccattgtc aaccccaata aaatattgat
     1681 tattgagcac cataactgat gggaatgagc cttcggaatt accaaaatta actactggtt
     1741 cggcttctaa acacatcaaa tggatagtgc cgtcgggata atcgtggtat gcttgtccta
     1801 aaacaaaacc tgcctctgcc acatcaaaag gagtaataaa gtgtgaaaca tccaacaaat
     1861 ggatggggtt tttacattgc ttcaaaattg aaacttttag agaggataca tagtaatctc
     1921 tcaattccat atctgatgtt aacgtaataa tttgcatttg ttgaaaattc ctgaactaag
     1981 taactaaatt atttcctaat tttgttcaaa agtaagattt atcatagaat taagtgcaat
     2041 aatcattata aattatcaaa ttgcaagaaa gagttattga aattg
//
LOCUS       DAOQVQ010000444         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_68019_length_2080_cov_3.809383, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000444 DAOQVQ010000000
VERSION     DAOQVQ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68019_length_2080_cov_3.809383"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="PKN22_10695"
     CDS             complement(<1..1363)
                     /locus_tag="PKN22_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNS43216.1"
                     /translation="MKILSVPQLKNVDALTVERQEITSHDLMERVAGECMGAIFCHVD
                     QDEEVVVFCGMGNNGGDGLAIARLLFKWHAAVRVVLVKHKDKFTPDTQKNLDKLPAAL
                     KQNLLVIEKEEDLKKIKLRPKSVIIDALLGTGVNKPVDGILKKVIAQINMHPSKIISI
                     DTPSGLLMDDSSQEFIESCVHADLVLTLQLPKLAFLLPDNKHVVNEFKIIDVGFDKRA
                     ILEQESSFYFMQKSDILPLIRPRNTFSHKGLFGHALLLAGSKGKTGAAIIAAESCLRS
                     GAGLLTVHSVDKVTNALAIRLPEAMTSTDGFDNHITHFDNFSKYSVIGFGPGVGKHMD
                     TQQVLKSIIYQHQGHLVIDADGLNILAENKTWLEFLPKETILTPHPKEFERLVGSFSN
                     SFERLDKARHFAARHQCILVLKDAFTHICMPDGSVVFNSTGNPGLAKGGSGDCLTGII
                     VGLL"
     gene            1504..>2080
                     /locus_tag="PKN22_10700"
     CDS             1504..>2080
                     /locus_tag="PKN22_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNS43217.1"
                     /translation="MEEIEIYGAREHNLKDIDLKIPRNKLVVFTGLSGSGKSSLAFDT
                     IFAEGQRRYLETFSAYARQFLGGLERPDVDKIDGLSPVIAIEQKTVSRSPRSTVGTIT
                     ELYDFLRLLYARVGTAYSYISGEKMVKYSDEQVVNLILEHYSGKKVVLLAPVVKGRKG
                     HYRELFEQIQKNGYTKVRVDGEIVDIQKGMKL"
BASE COUNT          625 a          425 c          413 g          617 t
ORIGIN      
        1 caagaagtcc aacaattatt cctgttaaac aatcgccgct gccacctttt gccaatccag
       61 gattccccgt agagttaaac acaacgcttc catcgggcat gcaaatatga gtaaaagcat
      121 cttttaacac caaaatacat tgatggcgtg ctgcaaaatg acgtgcttta tccaagcgct
      181 caaaactatt ggaaaacgaa ccaaccaagc gctcaaattc cttcggatga ggtgttaaaa
      241 tggtttcttt gggtaaaaat tccaaccatg ttttattttc tgccaaaata ttcaacccgt
      301 ctgcatcaat taccaaatga ccttggtgct gataaatgat ggatttcaaa acctgttgtg
      361 tatccatgtg tttgccaacg ccgggaccaa aaccaataac gctgtatttt gagaaattgt
      421 caaaatgagt aatgtggttg tcaaatccat cggtgctggt cattgcctct ggtagtcgaa
      481 tggccaaagc attagtaact ttatctaccg aatgtactgt aagcaatccc gcaccactac
      541 gcaaacacga ttcggcagca ataatggcgg cgccagtttt tcccttgctt ccagctaaca
      601 acaaggcatg accaaacaat cctttgtggg aaaacgtatt gcgcggacga attaacggaa
      661 gaatgtcgct tttttgcatg aaataaaaac tcgattcttg ctccaaaatt gcccttttat
      721 caaaaccaac gtcgataatt ttaaattcat tgaccacatg tttattatcg ggtagcaaga
      781 aagccaattt tggtaattgc aaagtcaaca ctaaatcggc atgcacgcaa ctttcaataa
      841 attcttgcga agaatcatcc atcagcaagc cacttggcgt gtcaatactt ataattttgc
      901 ttggatgcat gtttatttgt gcaatcactt ttttgagaat accatccact ggtttgttaa
      961 cgccagtgcc taatagtgca tcaatgataa ccgatttggg tcgaagtttt atttttttca
     1021 aatcctcttc tttttcaata acaagaaggt tttgttttaa agcagctggt aatttgtcta
     1081 aatttttttg cgtatcgggt gtaaatttat ctttgtgttt caccaataca acgcgcacag
     1141 cagcatgcca tttaaacaac aaacgcgcaa ttgctaaacc gtcgcctccg ttgtttccca
     1201 ttccacaaaa aacaactact tcttcatctt ggtctacatg acaaaaaatg gcgcccatgc
     1261 attcacccgc aacacgctcc attaagtcgt gtgaagtgat ttcttgacgc tccaccgtga
     1321 gagcatcaac atttttaagt tgaggtacag acaggatttt catagtggtc gaatttatag
     1381 taaaaatacg gtgttttttt aaataatcaa aaaatagtgt gtttttcggg caaatttgtt
     1441 gcttgattgt tccagcacaa ttgcgtaatt ttgtacaata aaaaaagtag cgagcaagca
     1501 ataatggaag aaattgaaat ttacggcgca cgagaacaca atttgaagga tatcgacctt
     1561 aaaattccgc gcaacaaact ggttgtattt actggtttga gtggtagtgg caaatcttcg
     1621 ttggcctttg acaccatttt tgcagagggg caacgccgtt atttggaaac tttttccgct
     1681 tatgctcgtc agtttcttgg gggcttggag cgtccggatg tggacaaaat tgatgggttg
     1741 agccctgtta ttgccattga acaaaaaacg gtttctcgta gcccccgctc aaccgttgga
     1801 acaatcaccg agctatacga ttttctccgc ttgctgtatg cacgtgtggg caccgcctat
     1861 tcttacattt cgggtgagaa aatggtcaag tattccgatg aacaagtggt taacttaatt
     1921 ttagaacatt actcaggaaa aaaagtggtg ttgttggcgc cagttgtaaa aggaagaaaa
     1981 gggcattacc gtgaattgtt tgaacaaatt caaaaaaacg gatacaccaa agtgcgtgtt
     2041 gatggtgaaa ttgttgatat tcaaaaggga atgaagctcg
//
LOCUS       DAOQVQ010000445         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_68692_length_2068_cov_3.303030, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000445 DAOQVQ010000000
VERSION     DAOQVQ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68692_length_2068_cov_3.303030"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKN22_10705"
     CDS             <1..364
                     /locus_tag="PKN22_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNS43218.1"
                     /translation="MVVSEFANVLLRRDYNQWITNNKFVDKDFKKDFVGIKEYENSVE
                     TITISINKIFKLPNLVFVGDNFNAIDKSAILKNFKKVDFNDAYYAQLAILNKYKVVTN
                     DKDYQKLDLYIDIITTQV"
     gene            514..>2068
                     /locus_tag="PKN22_10710"
     CDS             514..>2068
                     /locus_tag="PKN22_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43219.1"
                     /translation="MKFNKTSRTFLIGLLLTSFITINNCQAQIQKGLDIDGEAAEDYS
                     GWTVSMPDVNTVAIGAPLNDATGSNAGQVRVYSWNGSAWAQKGLDIDGEAIDDQFGSA
                     ISMPNANTVAIGANGNDGTGSSAGHVRVYSWNGTAWTQKGLDIDGEAAGDQSGFSVSM
                     PDENTVAIGALFNDGSATNAGHVRIYSWNGTAWVQKGLDIDGEAANDQSGYSISMPDA
                     NTVAIGAIYNSGAGFEFGHTRIYSWNGTAWVQKGLDIDGEAANDRSGWSVSMGDANTV
                     AISARYNDGNGSNSGHVRVYSWNGTTWTQQGMDINGEVAGDHSGWSVSMANANTLAIS
                     AIYNDGNGTDAGHTRIYSWNGSAWMQKGMDIDGEVAGDMSGYCVSMGDANTVAIGARF
                     NDGSATNAGHVRVFSMCTATTGTDTQTACGSFTWIDGNTYTANNNTATHTIVGGAANG
                     CDSIVTLNLTINNPTTGTDTQTACGSFTWIDGNTYTSSNNTATHTIVGGAANGCDSIV
                     TLNLTINNST"
BASE COUNT          699 a          315 c          466 g          588 t
ORIGIN      
        1 aatggttgtt tctgagtttg caaatgtttt gcttcgtaga gattataatc aatggataac
       61 aaataataaa tttgtagata aggattttaa aaaagacttt gtaggaataa aagagtatga
      121 gaattctgtt gaaactatta caatatcaat aaataaaatt tttaaactac caaatttagt
      181 atttgttgga gataatttta atgcaattga taaaagtgct atattgaaaa attttaaaaa
      241 agttgatttt aatgacgcat attatgctca attagcaatt ttaaataagt ataaagttgt
      301 cactaatgac aaagattatc aaaaattgga tttatatatt gatataataa ctactcaggt
      361 ttaggagtat taccactaac taaccaataa actccaaact ccagtttgaa aaagtactga
      421 aaacatatac ctccttcaaa agtcttaatt aaattaacaa aatacttacc actatttcat
      481 tacatttgag gcaacattaa gaaacattaa cttatgaaat ttaataaaac tagtcgtact
      541 tttttaatag gattattgct aacttccttt ataacaatta ataattgcca ggcgcaaatt
      601 caaaaaggat tagacattga tggcgaagct gccgaagatt acagtggttg gaccgttagt
      661 atgccagatg taaatacagt agctattgga gccccattaa atgacgcaac aggatcgaat
      721 gcaggtcaag tcagagtata tagttggaat ggaagtgcat gggcacaaaa aggattggat
      781 attgacggag aagctattga tgatcaattt ggaagtgcta taagcatgcc taatgccaac
      841 actgttgcta ttggcgcaaa tggaaacgat ggaacaggtt cgagtgcagg gcatgtacgt
      901 gtgtatagtt ggaatggtac tgcatggact caaaaaggat tggatattga tggtgaagct
      961 gcaggagatc aaagtggatt ttcggtcagc atgcccgatg aaaacactgt tgctattggt
     1021 gcgttattta atgatgggtc ggctacaaat gctggtcatg taagaattta cagttggaat
     1081 ggaactgctt gggtacaaaa agggttggat attgatggtg aggctgcaaa cgaccaaagt
     1141 ggctattcaa ttagtatgcc cgatgccaac actgtagcaa ttggagcgat ttataatagt
     1201 ggagcaggat ttgaatttgg gcatacaaga atttatagtt ggaacggaac tgcttgggtg
     1261 caaaaaggat tggatattga cggtgaggct gcaaacgacc gcagtggttg gtcggtgagc
     1321 atgggagatg caaatactgt ggctatctca gcacgatata acgatggaaa tggttctaat
     1381 tctggacatg tacgtgtgta tagctggaat ggaacaactt ggacacaaca aggaatggat
     1441 attaacggag aagttgcagg tgatcatagt ggatggtcgg taagtatggc caatgccaat
     1501 acacttgcaa ttagcgccat ttacaacgat gggaacggaa ctgatgccgg acatacacga
     1561 atttatagtt ggaacggatc tgcttggatg caaaaaggaa tggatatcga cggagaagtt
     1621 gcaggtgata tgagtggcta ttgtgtgagc atgggtgatg ccaatacggt ggcaattggt
     1681 gcacgattca acgatggttc agctacaaat gctggccatg tgcgcgtatt ttcaatgtgt
     1741 acagccacca caggaacaga cactcaaact gcttgcggtt cttttacatg gattgatgga
     1801 aacacttaca cagcaaacaa caacacagct acacatacca ttgttggcgg tgcagcaaat
     1861 ggttgtgatt caatagttac tttgaactta actattaata atcctaccac aggaacagac
     1921 actcaaactg cttgtggttc ttttacatgg attgatggaa atacttatac atcaagcaac
     1981 aacacggcta cacataccat tgttggcggt gcagcaaatg gttgtgattc aatagttacg
     2041 ttgaacttaa cgattaataa ttccacca
//
LOCUS       DAOQVQ010000446         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_68806_length_2066_cov_3.600199, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000446 DAOQVQ010000000
VERSION     DAOQVQ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68806_length_2066_cov_3.600199"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..493
                     /locus_tag="PKN22_10715"
     CDS             164..493
                     /locus_tag="PKN22_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43220.1"
                     /translation="MIRILFAAFLSFGIHFITFSQTSYSFSFKGFVSDFNELESKILK
                     IDGVLSCKYRYKDEKQAGEILFELKPYEKTYDDKGNLINPSPLIQIKELFYQENLEPI
                     ELVELKN"
     gene            499..>2066
                     /locus_tag="PKN22_10720"
     CDS             499..>2066
                     /locus_tag="PKN22_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43221.1"
                     /translation="MLLRILLLFALLVTVVIGYSQSDNCSSATTLTVGATCVGTSGTT
                     IGATQTIPGCTGNADDDVWYQFVATSTSHTITVAPSSGMDAVVQLFSGSCSVLQSLSC
                     KDNAFSGGTETITYTNFTIGQTYRIRVYDYYTGSGSGTFTICVTGAPSPPANDNCSNA
                     TTLTVNASCVTTSGTTVGATPSSFAGCVGDADDDVWYQFVATNPVQTVTVTPTSGIDL
                     IVGVYSGTCSSLISKACIDNTYSGGTESMQLVGLTVGATYYVRVYDYYTDNRGTFNIC
                     VTGTPTPAPINDECVDAIQIPTVTSECDYMEFTTVGATANMSDPTPTACAGAINPPYA
                     GGFGTNSHDVWFAITVPPSGNLTINIKPNMGAGKINDGVMALYSGSCAGLTQIACSEN
                     YNYPGTAHDLQPMLNQTGLTPGSTVYLRYWGYGTSQGTFGICVTTNSNDACANALYIC
                     DINGYSASTSPAFTPDRPGNMHGNNETAAGVNQPDGVNTGGPFGYYPYPGTIPGPYSS
                     PYLDVNIENNPWIR"
BASE COUNT          628 a          379 c          390 g          669 t
ORIGIN      
        1 gatggtactg ctaggttact ggtgggagag taagtcgacg ccacttttta aaagcccaat
       61 caaatagatt gggctttttt tttgactata ttaaaaaaat aatttttaca aaataatata
      121 ttcttgttat ttttttatac attcgtgttt taactattac taaatgatac gtattttatt
      181 cgctgctttc ttgtcttttg gcattcattt cataactttt tctcaaacaa gttactcttt
      241 ttcatttaaa ggatttgtat ctgatttcaa tgaattagaa agtaaaatct tgaaaattga
      301 cggtgtttta tcgtgtaaat atcgttacaa agatgaaaaa caagcaggtg aaattctgtt
      361 tgaattaaaa ccgtatgaaa aaacatacga cgataaagga aatctaatca atccttctcc
      421 acttattcaa attaaagaac tattttatca agaaaattta gagccaattg aattggttga
      481 attaaaaaac taaacaatat gctacttcgt attttactac tatttgcttt gctggtaaca
      541 gttgttattg gctattcaca atcagataat tgctcatcag ctacaacatt aaccgttggt
      601 gccacatgtg ttggaacgag tggaacaacc attggtgcaa ctcaaactat tcctggatgt
      661 acaggtaatg cggatgatga tgtttggtat caatttgtag caacaagcac cagtcacacc
      721 attacggttg ccccatcgtc tggaatggat gctgttgttc aattgttttc tggaagttgc
      781 tctgtacttc aatcgctttc atgtaaagac aatgcttttt caggtgggac agaaacaatt
      841 acatatacaa atttcacaat aggtcaaacc taccgtattc gtgtttacga ttattataca
      901 ggctctggct ctggcacatt tacaatttgt gtaactggtg caccatctcc acctgcaaat
      961 gacaactgct ctaatgcaac aactttaaca gttaatgcaa gttgcgtaac aacttctggc
     1021 acaacagtgg gggcaactcc ttcctctttt gctggatgtg taggcgatgc agatgacgat
     1081 gtgtggtatc aatttgttgc tacaaaccct gtgcaaactg ttactgttac gccaactagc
     1141 ggaatagatt taatagttgg ggtgtattct ggtacatgta gttcactaat atccaaagct
     1201 tgtatcgaca atacctatag cggtggaaca gaaagcatgc aattagttgg tttaactgtt
     1261 ggagcaactt attacgttag agtttatgat tattataccg ataatagagg tacatttaat
     1321 atttgtgtta ctggaacacc aactcctgct ccaataaacg atgaatgtgt tgatgcaatt
     1381 caaataccaa cagttacttc tgaatgtgat tacatggaat ttacaaccgt tggagctaca
     1441 gctaacatgt cagatccaac tcctactgca tgtgcggggg caatcaaccc tccttatgct
     1501 ggtggttttg gaaccaactc acacgacgtt tggtttgcaa taacagttcc tccaagtggt
     1561 aacttaacca tcaatattaa accaaatatg ggagctggta aaattaacga tggtgtaatg
     1621 gctttgtatt ctggaagttg tgcaggatta actcaaattg cttgttccga aaattataac
     1681 tatcctggga ctgcacacga tttgcaaccc atgttaaatc aaactggact tacaccaggt
     1741 tcaactgtct atttacgtta ttggggttat ggcacttcgc aaggaacttt tgggatttgt
     1801 gttacaacga atagcaacga tgcgtgtgct aatgcgcttt atatttgtga tattaacggt
     1861 tacagcgcat caacctctcc tgcatttaca cctgacagac cggggaatat gcatggaaat
     1921 aacgaaacag ctgcaggagt aaaccaacca gatggtgtaa acacaggagg tccttttgga
     1981 tattatcctt atcctggaac aattcccgga ccttactctt caccgtattt ggatgttaat
     2041 attgaaaata atccttggat acgatt
//
LOCUS       DAOQVQ010000447         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_69763_length_2048_cov_3.430507, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000447 DAOQVQ010000000
VERSION     DAOQVQ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69763_length_2048_cov_3.430507"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKN22_10725"
     CDS             complement(<1..549)
                     /locus_tag="PKN22_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNS43222.1"
                     /translation="MFRLALIIGSLLIVVGNAFSQSDSLQKSDSMTSQQLDEVIISTR
                     KILADGMAKPLSGIEGFLNKMDALNLVQRGGYASEPYINGMSTERSVVTIDGMRIYGA
                     CTDKMDPVTSYVETTNLEKATVHGGSTNSATGASIAGSLDLVRKRAGFGVQQFKGAVF
                     TGIETNNWQKIGGTSLSYSRPKF"
     gene            complement(612..1505)
                     /locus_tag="PKN22_10730"
     CDS             complement(612..1505)
                     /locus_tag="PKN22_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43223.1"
                     /translation="MRTLNFFKYMLLGVAIIGLNACKKEGCTDKNAINYSVKAKKDDG
                     TCKYETQETETYVLAKSVVDGDYTLEYYTEKGKFLTGYNAIKVKIKKADGTYVENVSM
                     TWAPLMHMTSMSHACPASSLTNKGTYNEGYMVFQMASNDTEYWDITLNYTINGVEHSN
                     TSVVVVESAPKRIVQSFKGTDNVKYILALVEPKSPIVGTNDVKMVLYKMASMTSFLPV
                     ESYSVKIDPRMPSMGNHSSPNNVHLTQGSDEFYYGKLNLTMTGYWKINLQLLDNAGTV
                     LKGEEVTSTVEASSIYFEVEF"
     gene            complement(1608..1922)
                     /locus_tag="PKN22_10735"
     CDS             complement(1608..1922)
                     /locus_tag="PKN22_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43224.1"
                     /translation="MITSVYNYDQELFISLFCENTDKPEMHCDGKCAMSKMQKEQKED
                     DANKTLSKLQIETIALVQAFNFDAYYSFFTTEEPIHLLPAYSNNYSFEYADLTAKPPT
                     SF"
BASE COUNT          641 a          394 c          316 g          697 t
ORIGIN      
        1 gaatttaggg cgcgaatagg ataaacttgt acctccaatt ttttgccagt tattggtttc
       61 aattccagta aaaacagctc ctttaaattg ttgtacacca aaaccagctc tttttcgcac
      121 taggtctaaa ctaccagcaa tggatgcacc tgttgcagaa ttggttgagc ctccgtgcac
      181 tgttgctttt tccaaattcg ttgtttctac ataagaggta acaggatcca ttttatcggt
      241 acaagctcca taaattcgca taccgtcgat ggtgacaacg ctacgttcgg tggacattcc
      301 attaatatat ggttccgaag cataaccacc acgttgaacc aagtttaaag catccatttt
      361 attcaaaaat ccttcaattc cagaaagagg tttagccatt ccgtctgcta aaatttttcg
      421 cgtactgata attacctcat ccaattgttg cgaggtcatt gaatccgatt tttgcaacga
      481 atcggattgt gaaaaggcat tgccaacaac aattagcaaa ctaccaataa ttagtgctaa
      541 cctgaacata tcaatcgata ttaatgagcg acccccatca tgaaaagagt cgctcaatta
      601 ataaataaac cttaaaactc tacctcaaaa taaatgctgc tagcttcgac agttgaggtc
      661 acttcttcgc cttttaaaac agtgccagca ttgtctaaca attgcaagtt gattttccaa
      721 taacctgtca ttgttaaatt caatttaccg taataaaact cgtcacttcc ttgagttaaa
      781 tgtacgttgt taggtgaact gtgatttccc atacttggca tacgtggatc aatttttaca
      841 gaataagatt caactggtaa aaaactagtc attgaagcca ttttataaag taccattttc
      901 acgtcgtttg taccaacaat tggtgatttt ggttcaacca atgccaaaat atacttcaca
      961 ttatcagttc ctttgaacga ttgaacaatg cgttttggtg cgctttcaac tacaacaaca
     1021 cttgtattgg aatgttctac tccgttgatg gtgtaattca aagtaatgtc ccaatattct
     1081 gtatcgtttg atgccatttg aaaaaccata tacccttcat tgtaagtccc tttgttagta
     1141 agcgacgaag cagggcaagc gtgactcata ctagtcatgt gcataagtgg cgcccaagtc
     1201 atagaaacat tttcaacata cgttccatct gcttttttga tttttacttt aatggcattg
     1261 taaccagtta aaaacttacc tttttcggta taatattcca acgtataatc gccatcaaca
     1321 acgctttttg ctaacacata cgtttctgtt tcttgtgttt catacttaca agttccatcg
     1381 tcttttttgg ctttaacgct ataattaatt gcatttttat cggtacaacc ctctttttta
     1441 caagcgttca accctataat tgctacccct agcaacatgt atttaaagaa atttaaagtt
     1501 ctcatctttt caaaatttaa ttcattatta ttttggtaat ggttgtataa accccattac
     1561 actccgtaaa aacgtgtgtt tatttcaact gaaataaaat caatgaatta gaaagatgtg
     1621 ggtggttttg ctgttaaatc agcatattca aaagaatagt tattgctata tgctggtaat
     1681 aaatgtatcg gttcttcagt agtgaaaaac gaataatagg catcaaaatt aaacgcttgc
     1741 accaaagcaa ttgtttctat ttgtaatttg cttaaagttt tattggcatc gtcctctttt
     1801 tgttcttttt gcatttttga catggcacat tttccatcac aatgcatttc tggcttatcg
     1861 gtattttcac aaaaaaggga aatgaataat tcttgatcat aattgtaaac agatgtaatc
     1921 accgtgtttt ttacgattcc aaatagaaag gcaaaaacaa gaaattggac tactattttc
     1981 tgaattgttc tcaatttaca attagattaa aagtataaca ccacaaaagt aataaattat
     2041 ttaatata
//
LOCUS       DAOQVQ010000448         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_69780_length_2048_cov_2.863522, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000448 DAOQVQ010000000
VERSION     DAOQVQ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69780_length_2048_cov_2.863522"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2048)
                     /locus_tag="PKN22_10740"
     CDS             complement(<1..>2048)
                     /locus_tag="PKN22_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43225.1"
                     /translation="NPDGTLATNQSEGLDRDYITQWSYGIGETFTLISPNVKGGGSFQ
                     LGGSQFESAIENSDLSAQAKQDVANYGAYWGDQPFTSGPVYIGMVVFVLAFLGLFFWK
                     SKMKWVFLGVSLLAIALSWGKNFMVLTNLFIDYVPGYNMFRTVTIILILVELCAVALA
                     MFFIDDFIKNKEQYVANKKKLLIVLGGLVVFSFVMRSIGGSIDSFSGAAEKDQLAQLE
                     GQITSQIKSMDPAVLMSNYQLDINNAQQVKEFVAMQVKQSEERIDDLKTIRMQIYKDS
                     WMRTTVFAFLTGLVLFLFVSTSMTPIILSISLVALTAFDLIPVAYDYLGSQTDVNGNY
                     KYWEDAGLTAYPVAANEGDYQIMEMEMATNPSLAAKIEKAGREANSSAVELGYTGQTR
                     TNMIDAAKFSALNFNSNYRVFDQMGGFNSSRSSYFHKGLGGYHGAKLRNFQNLVDFHI
                     AKGNNTVFDMLNVRYIIQQDQQGAVARVNPNAAGNAWLVKKVRTYPSPNEEILALGTQ
                     FFVENKGVGTLFVNGKETPSATVTGNERLQYLVKGSNDTITVPLSNGMKEGMEVAFVM
                     DANGKTDLVMPTVFDTEEGKKSFLQLTSIKVKETFDLKEEAVMLESEAKKLSSKTYSG
                     EGTVKMTSYLPNNVKYTADLKDKQLVVFSEMYYDEGWKLLVDGKETPIVKVNYALRGA
                     "
BASE COUNT          635 a          455 c          301 g          657 t
ORIGIN      
        1 tcagcacctc tcaatgcgta attcactttc acaataggtg tttcttttcc gtcaaccaat
       61 aatttccaac cttcgtcgta gtacatttca gagaaaacca ccaattgttt gtcttttaaa
      121 tcagcagtgt attttacatt atttggcaaa taacttgtca ttttcacagt tccttcacct
      181 gaataagttt tagacgaaag tttcttagct tcactctcca acatcaccgc ttcttctttc
      241 aaatcaaatg tttcttttac tttgattgaa gttaattgca agaacgattt tttgccttct
      301 tcggtatcaa aaacagttgg cattactaaa tccgtttttc cgtttgcatc cattacaaaa
      361 gccacttcca ttccttcttt cataccgtta ctcaacggaa cagtaatcgt gtcattagag
      421 ccttttacca aatattgcaa acgttcattt cctgtaactg tagcggatgg tgtttctttt
      481 ccattaacaa acaaggtacc taccccttta ttttcaacga agaattgagt tcccaacgcc
      541 aaaatttctt cgttaggcga agggtatgtt ctcactttct taaccaacca agcatttcct
      601 gcggcattag gattcacacg tgcaactgca ccttgttgat cttgttgaat aatgtaacgc
      661 acgttcaaca tatcgaacac cgtattattc cctttagcaa tatggaaatc aaccaaattc
      721 tggaaatttc tcaattttgc tccgtggtaa cctccaagtc ctttgtgaaa atacgaagaa
      781 cggctgctgt taaatccacc catttggtca aaaacacggt agttgctatt gaaattcaat
      841 gcagaaaatt tagcggcatc tatcatattg gtgcgtgttt gccctgtata accaagttct
      901 acagcactgg aattagcttc tctccccgct ttttcaattt tagcagctaa agaaggattg
      961 gtagccattt ccatctccat aatttgataa tcgccctcat tggcagcaac cggataagct
     1021 gttaaaccag catcttccca atatttgtaa tttccattca catcggtttg acttcccaaa
     1081 taatcgtatg caacaggaat taaatcaaac gccgttaatg ctactaatga aatacttaaa
     1141 attattgggg tcattgaagt tgaaacaaat aaaaacaaca ccaatccagt taaaaatgca
     1201 aaaacggttg tgcgcatcca tgaatccttg taaatctgca tgcggatagt tttcaaatca
     1261 tcaatacgtt cttcggattg tttcacttgc attgccacaa actcttttac ttgttgtgca
     1321 ttgttaatat ccaattgata gttactcatc aaaactgctg ggtccatcga tttgatttga
     1381 gaagtaattt gaccttccaa ttgtgctaat tggtcctttt ctgcagcacc tgaaaagcta
     1441 tcaattgaac ctccaataga gcgcattaca aaagaaaaaa caaccaaacc tccaagcaca
     1501 atcaataatt tctttttatt tgccacatat tgttctttgt ttttaataaa atcatcgata
     1561 aagaacatgg ctaatgccac agcacataat tcaaccaaaa ttaaaatgat tgtaacggtt
     1621 ctaaacatgt tatatcctgg tacataatca ataaaaagat tggttaacac catgaaattt
     1681 ttaccccatg atagtgcaat agcaagcaat gaaaccccta aaaacaccca tttcatttta
     1741 gatttccaga aaaacaatcc taaaaaagcc aaaacaaaaa ccaccatacc aatgtaaacc
     1801 ggtccacttg taaacggttg atctccccaa taagcgccat aattggctac atcttgtttg
     1861 gcttgagcag ataaatcgga attttcaatt gcgctttcaa attgacttcc tcctaattga
     1921 aaagaacctc cacctttaac atttggtgaa atcaatgtga atgtttcgcc aattccatac
     1981 gaccattgtg tgatgtaatc tctatccaac ccctctgatt gattggttgc caaggttccg
     2041 tctggatt
//
LOCUS       DAOQVQ010000449         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_70230_length_2039_cov_4.015121, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000449 DAOQVQ010000000
VERSION     DAOQVQ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70230_length_2039_cov_4.015121"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKN22_10745"
     CDS             <1..837
                     /locus_tag="PKN22_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNS43226.1"
                     /translation="FNDAVITAGGATEYESYVDVVFQQDEVAHFISRKLYRYFVNHDL
                     TEDVEASVIPAMAAIMIANNYEILPVVEALFKSQHFYDVKLKGSCIKSPVELLFSLIN
                     GSLSTPSYDLATTSDMYLYIYGLGDNLGQPYGQPPSVSGWSAYYQAPNFTKLWVNSTH
                     IKTRMSLAYLFTIGSGIVINGNTFKVNALDFLNSLSNPSSANAVIDDMILVFLPKPLT
                     ATQRLILKSVLTGGQPDFEWTVQYNDYNSDPGNTTYSLPIKQKIETTLMRLFILPECQ
                     TF"
     gene            874..>2039
                     /locus_tag="PKN22_10750"
     CDS             874..>2039
                     /locus_tag="PKN22_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNS43227.1"
                     /translation="MALSSIGMPFVLNNIQLQTIQQKLFNFAKSAEDRVLVIIRLNGG
                     NDGLNTVIPIDQYDNLNIQRANILIPENQLLDLGQSNLSLHPSMAGMQTLFQQGQLGI
                     IQNVGYPDQNRSHFRSMDIWSAGSLDPYEHKGWLGRHFDDYYPNYPDDYPNTQYPHPF
                     AISMGYNVSETCQGLMANFSQAIANPLDSITLPNSNTVDDGTYYGSHMAYLQNLVDQT
                     NAYGQQIYNATNAGSTLSQLYDANNPLAVHLRYVARMISGGLQTKVYILNIDGFDTHS
                     GQVTQGNVTAGKHADLLKTLSDAVYAFQDDLNLLGLNQRVAGFTFSEFGRQIASNASF
                     GSDHGDAAPMFLFGQCITSQVIGSNPVISNTIENQAGVAMQIDFRDVYASLLKD"
BASE COUNT          622 a          385 c          400 g          632 t
ORIGIN      
        1 tttaatgatg ccgtaattac tgctggcggt gcaactgaat atgaaagcta tgtagatgtg
       61 gtgtttcagc aagatgaagt ggcacatttt atatctcgaa aattgtatcg ttattttgtg
      121 aaccatgatt taaccgaaga cgttgaagct tctgtcattc cagcaatggc agccatcatg
      181 attgccaata attatgaaat tcttcctgtt gtagaagctt tgtttaaatc gcaacatttc
      241 tacgatgtaa aattaaaagg ttcgtgtatt aaaagtccgg tagaattgtt gttttcatta
      301 atcaacggtt cactttcaac tccaagctat gatttagcaa caacatctga tatgtattta
      361 tacatctatg gtttgggtga taacttgggg caaccttatg ggcaaccacc ttctgtatct
      421 ggttggtcgg cctactacca agcacccaat tttacgaagt tgtgggtaaa ctctacacat
      481 atcaaaacac gcatgtcatt ggcttatttg tttacaattg gaagtggcat tgtaattaac
      541 ggtaatacat ttaaagtgaa tgcattggat ttcttgaaca gtttatctaa tccttcaagt
      601 gccaatgcag taattgacga tatgattttg gtgtttttac ctaaaccatt aaccgcaaca
      661 caacgcttga ttttaaagtc ggtgttaacg ggagggcaac cagattttga gtggacagtg
      721 caatacaacg attacaacag tgatccagga aacaccacct attcattgcc aattaaacaa
      781 aaaattgaaa caacactcat gcgtttgttt attttacctg aatgtcaaac tttttaataa
      841 gaccccatga aaagaagaaa ttttgttaaa aatatggcat taagttcaat aggaatgcca
      901 tttgtgttaa acaatatcca actacaaacc atccaacaaa aactctttaa ttttgcgaaa
      961 agtgccgaag atagagtgtt ggtaatcatt cgattaaatg gaggcaacga tggtttgaat
     1021 acggtaattc ctattgatca atatgacaat ttgaacattc aacgtgcaaa cattctcatc
     1081 ccagaaaatc aattattgga tttagggcaa agtaatttat cacttcaccc gtccatggct
     1141 ggaatgcaaa cactatttca gcaaggtcag ttaggaatta ttcaaaacgt gggttacccc
     1201 gatcaaaacc gttcacactt tcgttcaatg gatatttgga gcgcaggttc acttgatccg
     1261 tatgagcata aaggatggtt gggccgccat tttgatgatt attatcccaa ttatcccgac
     1321 gattatccca atacgcagta tccgcatccg tttgcaatca gcatgggata taatgtttct
     1381 gaaacatgcc aaggattgat ggctaatttt tcgcaagcaa ttgccaatcc attggattcc
     1441 atcaccttgc ctaacagcaa cactgttgat gatggaactt attatggatc acacatggcc
     1501 tatttgcaaa atttggtaga tcaaaccaat gcctatggtc agcaaattta caatgctacc
     1561 aatgctggaa gtaccttgtc gcaactatac gatgccaaca atccacttgc tgttcattta
     1621 cgctatgtgg cgcgtatgat ttctggtggt ttgcaaacca aagtgtacat tctaaatatt
     1681 gatggttttg acacgcatag tgggcaggta actcaaggaa atgtaacagc tggtaaacat
     1741 gcagacttgc taaaaactct ttcggatgca gtgtatgcct tccaagacga tttgaacttg
     1801 ttagggttaa atcaacgtgt ggctgggttc actttctcag aatttggtcg acaaattgct
     1861 tccaatgcat cgtttggtag cgaccacgga gatgcagcac cgatgttctt gtttggtcag
     1921 tgcattacgt cgcaagttat tggttcaaat ccagttattt ctaacaccat tgaaaaccaa
     1981 gcgggtgttg ctatgcaaat agattttaga gacgtgtatg cctcgttgtt aaaagattg
//
LOCUS       DAOQVQ010000450         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_70402_length_2036_cov_3.248359, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000450 DAOQVQ010000000
VERSION     DAOQVQ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70402_length_2036_cov_3.248359"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PKN22_10755"
     CDS             complement(<1..905)
                     /locus_tag="PKN22_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43228.1"
                     /translation="MKRKIQVVATFMLLFTSNSFGQYWLSNGSLNNGNLGHNLGTFTP
                     HPINIYTGGIYRAIFTTGGALSSFSNNTGDGLRIRNQGQFPSAGNLDLFTSNSNGQNE
                     THIVFGANGQISGQNNRFEFLAKTNQGFWFNLLNNNQYYKFASNNIVHAFVGNNRFWR
                     IGDQQDANNISAIRRLEVVENNWQFRLSRTNGLFTDFQTNSAGNLQILPQNGRVGINI
                     TSNPTANLDVNGNVRIRNVQAATPDALIVGTQAGASGDLNVRRLDFNGNSNTFLGGDG
                     QFHTVNATPPSANNGVSRSTLFNLN"
     gene            complement(919..1809)
                     /locus_tag="PKN22_10760"
     CDS             complement(919..1809)
                     /locus_tag="PKN22_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS43229.1"
                     /translation="MNPSFEEYYQCPTGSANIENCKYVFNPMCANSQAVGCISTPDYF
                     NTCASFGDNSNVPYSVNGFQQAKNGQAYIGFFDGTFYREYIQIKLSKSLAKGNTYPFS
                     FYANLANYSNITTNQVGLKFVQDSIIYWQNYLWQFMQPDWVSNEYITDTADWQLLQGE
                     YTAKGGEQWVIIGNFYPDSIFPSIIVDSTVPTAWLNCYYIFIDDFSVQSKPLQQPNVF
                     TPNNDGVNDFWITEGNVVSISITNRWGNVVFSSENGFSGWDGKDQQGKDCSAGVYYYV
                     LQNKKGDEINTHHGFITLIR"
BASE COUNT          642 a          373 c          335 g          686 t
ORIGIN      
        1 tggtttaaat taaatagcgt gcttcttgaa acgccattat ttgcacttgg tggtgttgca
       61 tttactgtat gaaactgtcc atctccacct aaaaaagtgt tggaattacc attaaaatcc
      121 aatcttctca cattcaaatc tcccgaagct ccagcttgcg tacctactat caacgcatca
      181 ggtgttgcag cctgcacatt tcggatgcgc acgttgccat ttacgtccaa attggcagtt
      241 gggttgcttg tgatgttgat accgactctg ccattttgag gtaaaatttg caaatttcca
      301 gctgagttag tttgaaaatc agtaaataat ccatttgttc gagataatcg aaactgccaa
      361 ttgttttcta caacttctaa tcttcggatg gcactaatgt tattggcatc ttgttgatca
      421 ccaattctcc aaaagcggtt attgcctaca aaagcatgca caatgttgtt tgatgcaaac
      481 ttataatatt ggttattgtt caatagatta aaccaaaatc cttgatttgt ttttgctaaa
      541 aattcaaatc tattgttttg tccagaaatt tggccatttg ccccaaaaac tatatgtgtt
      601 tcattttgcc cattagaatt tgaagtaaat aaatctaaat tacctgcaga tggaaattga
      661 ccttgatttc taatgcgcaa accatcaccg gtattgtttg aaaatgaact taacgcgcca
      721 ccagtggtga atattgctct atatataccg ccagtataaa tattaatagg atgaggagta
      781 aatgtaccaa ggttatgtcc tagattacca ttgtttaaac ttccattaga aagccaatat
      841 tgcccaaaac tattggaagt gaatagcagc ataaaagttg ctactacttg tatttttctt
      901 ttcataatta taaggttttc accttatcag tgttatgaac ccatggtgtg tgtttatttc
      961 atcgcctttt ttgttttgta gcacatagta atatacacct gcactacaat cttttccttg
     1021 ttggtcttta ccatcccagc ctgagaaccc gttttcggat gaaaacacca cattacccca
     1081 acgattggta atgctaattg aaacaacatt tccttctgta atccaaaaat catttacacc
     1141 atcattgttg ggcgtaaaaa cattgggttg ttgtaatggt tttgactgaa ctgaaaaatc
     1201 gtcaatgaat atatagtaac aatttaacca tgctgtgggt actgttgaat caactattat
     1261 gctaggaaaa atggagtcgg gataaaaatt ccctataatt acccattgct ctcctccttt
     1321 agcggtgtat tctccttgca ataattgcca atctgctgta tcggtaatat actcattgct
     1381 tacccaatct ggttgcataa actgccacaa atagttttgc caatagatga tagaatcttg
     1441 cacaaatttt aaaccaactt gattggtggt aatgttgcta taattagcca aattagcata
     1501 aaaagaaaaa ggataggtat tcccttttgc taagcttttt gacaacttta tttggatgta
     1561 ttccctataa aaagtaccat caaaaaagcc aatataagct tgcccatttt tagcttgctg
     1621 aaatccattt acagaataag ggacattaga attatcacca aaactagcac acgtattaaa
     1681 ataatcggga gttgaaatac aaccaacagc ttggctattt gcacacatag ggttaaatac
     1741 atatttacaa ttttcaatgt ttgctgagcc agttgggcat tggtaatact cctcaaaact
     1801 tgggttcatt accaaatttt gtgcattagc aagcaagcaa tataaaagaa atgatataaa
     1861 cacaaaggtt ctttgcactg ataagatatt gtttaaaaaa ttaaattaat ttcttcttgc
     1921 ttcgcccaag aaattgcgcc ttgcaataaa aagacgtgta gcttcattat agttgttaac
     1981 gaatatacaa aatttgttta gttatggact cataagtagt atgtttttgt agtttt
//
LOCUS       DAOQVQ010000451         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_70722_length_2030_cov_3.872911, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000451 DAOQVQ010000000
VERSION     DAOQVQ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70722_length_2030_cov_3.872911"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1481)
                     /locus_tag="PKN22_10765"
     CDS             complement(165..1481)
                     /locus_tag="PKN22_10765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNS43230.1"
                     /translation="MKKIAVVGTGYVGLVTGTCFAETGNQVTCIDIDAQKVERMLNGE
                     IPIYEPQLDTLFERNLKAGRLKITTDLKEGIQNAEIVFLALPTPPGEDGSADLSYILG
                     VAEHIGKIIEDYKVIVDKSTVPVGTAEKVRAAILKSSKVDFDVVSNPEFLREGFAVED
                     FMKPERVVVGTSSEKAKKIMGELYQPFVRQGNPIIFMDEKSAELTKYAANSFLAMKIT
                     FMNEVANFCELVGADVDMVRIGMGSDSRIGKRFLFPGIGYGGSCFPKDVQALVKSGKD
                     ENFNFQILESVMQINETQKTVLLPKMKNYFKGNLSGKKIAVWGLAFKPNTDDIREAPA
                     LYMIDGLLKEGAQIAAYDPEAMENVKKQLGDTITYCENEYETLQNADALLICTEWGIF
                     RNPDFNRLKELMNNHAIFDGRNLFDTETVAKQGIYYQSIGREIVGK"
     gene            complement(1500..>2030)
                     /locus_tag="PKN22_10770"
     CDS             complement(1500..>2030)
                     /locus_tag="PKN22_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43231.1"
                     /translation="NNIVYESGYMYPSKFRPLSVGNTFIIRYNVSVYNDILGQVAYSA
                     SGSKTNGNSNWFKDITISNLKLNDAIDVTLTGSNLNFSNDLLNCQLIYSYATTGSPET
                     RLLATFNGYNSSTNKVSLISTGNDLLSLFKDNPYGDLYLKFSFSQNAATDNYANLTYT
                     VAFSYNYSYTEMSSSK"
BASE COUNT          653 a          400 c          308 g          669 t
ORIGIN      
        1 aacgtaacac ctgccacgtt taatttcagt gtaggcttta cctataaatt ggacaaattc
       61 cgcatcgatc ctgagaaaat gtcgaagcgc aaaaaacaat ggatagaaac catccaagaa
      121 gacaaaaaag acattgaaaa gttagagaaa ggagaataaa atttttattt acctacaatt
      181 tctcgtccaa tggattgata ataaatccct tgtttagcaa cggtttctgt atcaaacaaa
      241 ttacgtccat caaaaatagc gtgattgttc attaactctt tcaaacggtt aaaatcagga
      301 tttctaaaaa ttccccattc cgtacaaatc aataaagcat cagcattttg aagcgtttcg
      361 tattcgtttt cgcaataggt aatggtatcg cccaattgct ttttaacatt ttccatcgct
      421 tctgggtcgt aggcagcaat ttgcgcacct tctttaagca aaccatcaat catgtataag
      481 gctggtgctt cacgaatgtc gtcggtattt ggtttaaatg ccaatcccca aacagctatt
      541 tttttacccg aaagattgcc tttaaaatag tttttcattt tgggtaataa aactgttttt
      601 tgagtttcat tgatttgcat caccgactct aaaatttgga agttgaaatt ttcgtcttta
      661 ccacttttta ccaatgcttg cacgtctttt ggaaaacaag aaccaccgta gccaatgcct
      721 ggaaacaaaa agcgttttcc aatacgcgaa tcagagccca tacctatgcg caccatgtcc
      781 acatcagcac ccactaattc acaaaaattt gccacttcat tcatgaacgt aattttcatt
      841 gctaagaatg aattggcagc atattttgtc aattcagcag atttttcatc cataaaaatg
      901 atgggatttc cttgacgcac aaatggttga tacaattcac ccataatttt tttagctttt
      961 tcagaacttg ttcccactac cacacgttcg ggtttcatga aatcttctac tgcaaatcct
     1021 tcgcgtaaaa actcagggtt tgaaaccacg tcaaaatcaa ctttagaact ttttaaaatt
     1081 gcagcacgca ctttttcagc cgtaccaaca ggaactgtac ttttatctac tattactttg
     1141 taatcttcaa taatttttcc gatgtgttca gcaacgccca atatgtaaga taaatccgcc
     1201 gaaccatctt ctccaggagg agtaggcaga gccaaaaaaa caatttcagc attttgaata
     1261 ccttccttta agtcggtagt aatttttaat cttcccgctt ttaaattgcg ttcaaacaag
     1321 gtgtctaatt gcggttcgta aattggaatt tcgccgttca acatgcgctc cactttctga
     1381 gcatcaatgt ctatacacgt tacttgattg cctgtttcgg caaagcaagt tcctgttacc
     1441 aaccctacat atcctgttcc aacaactgca atttttttca tgttaagtaa atctttttat
     1501 tattttgatg atgacatttc tgtgtatgaa taattgtaac taaatgctac tgtgtaagtt
     1561 aaatttgcat aattatctgt agcagcgttt tgcgagaaac taaactttaa atacaaatca
     1621 ccataaggat tatctttaaa taaactcaac aaatcattgc ctgtagaaat taaagacact
     1681 ttgttggttg atgagttata tccgttaaat gttgctaata atcttgtttc aggtgatcct
     1741 gttgttgcat aagaatagat taactgacaa ttcaataaat catttgaaaa attcaaatta
     1801 ctacccgtaa gtgtaacatc aatagcatca tttaatttta aattggaaat agtaatatct
     1861 ttaaaccaat ttgaatttcc attcgtttta gagccacttg cactatatgc aacttgacct
     1921 aaaatatcat tgtaaacaga tacgttatag cgaattataa atgtgtttcc aaccgaaaga
     1981 ggtctaaact tgctgggata catataacca gattcataga caatattgtt
//
LOCUS       DAOQVQ010000452         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_71281_length_2020_cov_3.746565, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000452 DAOQVQ010000000
VERSION     DAOQVQ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71281_length_2020_cov_3.746565"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKN22_10775"
     CDS             complement(<1..220)
                     /locus_tag="PKN22_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43232.1"
                     /translation="MKATKSNSILEQLRTGDETPLFDLYKLHRKEFIVWSKATFKISE
                     DQAKDAFQEAILDFHQNVISGHLVELTSS"
     gene            568..>2020
                     /locus_tag="PKN22_10780"
     CDS             568..>2020
                     /locus_tag="PKN22_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43233.1"
                     /translation="MLGQCPSGASPYAGETTILSNQVFCMTADFTVSSLTIESGGQLY
                     VANEKKLTVTGNTLVSGNLTFADEAGGVMSTLTIGNGTISNAIVNLGKKSYLTVSGGI
                     TQNLSTSPYVSQIEMWSGSIVEDCGTYYQSSRTYPFIKFLGSEAVKSYFIVKGAATSA
                     GPGAHFSHDNSIVVVAMGSVSGVTGGLSVYCGEYATPGTCPSWPSGLSNNPLDCYNIT
                     TILQNLPEPETACHENTFVKSTDPNTLEYDNIVCAFHATILKESNGTIKVWGDEAHAN
                     GSTHVLAPVEVTPANGYNYTGTPLRVTAGSYAGSTLSSVVHQFALLTTTGLHIWGVPG
                     TLINTGIKNTNAFGPITVNGNPTGLPTGVSPANVKMLFGAYRTLAIVTDDGHVWVLSF
                     NGEKNGDGTSQSASNNIIWHQVCTSSGITLKQVVAMRGNTNALMALSSDGKVYTWGSN
                     TYINSGGPANRTYATEITLPSGLTPKMIGMAPNG"
BASE COUNT          649 a          364 c          385 g          622 t
ORIGIN      
        1 cagagctcgt aagttctacc aaatgaccgg atattacatt ttgatgaaaa tccaaaatag
       61 cctcttggaa ggcatccttt gcctggtctt cacttatttt aaatgttgct tttgaccaaa
      121 caataaattc ttttctgtgc aatttataca aatcaaataa tggtgtttca tctccagtgc
      181 ggagttgttc caatatacta tttgatttcg ttgctttcat ttcgtgctat gctttaaaca
      241 attaaaatgc aagtttgtta ggtttaaaac aatatttaat tcttttgttt aatgaggtcg
      301 aaaataagaa ttatatactt acaaattcaa gtgtggtcta attaaaaaaa agtagggtaa
      361 acaaaagatt ttgctactaa ttatttgagt aacaaatgtt ttttttaaaa taattgaaaa
      421 aaagtaacca actaaacatc gttttaccct acatttgtta aaaataaatt gaattttatt
      481 tttttaccac tatttactca caaaactgaa aaagaaatga agacactatt tacattcgtc
      541 tttgtccttt gcgtaccact aacaattgtg ctaggacaat gcccatcagg agcttcccct
      601 tacgctggag aaacaaccat tctgtcaaat caggtgtttt gcatgacagc tgattttacc
      661 gtatcaagtt taaccattga atcgggagga caattgtatg ttgctaatga aaaaaaatta
      721 acagttactg gtaacacact tgttagcggc aacttaacct ttgctgatga ggcaggtggc
      781 gttatgtcaa ccttaaccat aggtaacgga accattagca atgccatagt taacttgggc
      841 aaaaaatcgt atttaacggt tagtggaggt atcacacaaa atttatccac atcaccctat
      901 gtttctcaaa ttgaaatgtg gtcgggctct attgtggaag attgtggtac gtattaccaa
      961 agctctagaa cttatccgtt tatcaaattt ttaggttccg aagctgttaa atcctatttt
     1021 atagtaaaag gagctgcaac tagcgctggt cctggagctc atttctcaca cgataatagc
     1081 atcgttgttg tggcaatggg gtctgttagc ggtgtaactg gaggtttgtc tgtttattgt
     1141 ggagaatatg caacgccagg cacatgtcca agctggccaa gtggtttgag taataacccg
     1201 ttggattgct acaatataac aaccatactt caaaatttac ccgaaccaga aacagcgtgt
     1261 cacgaaaata catttgtaaa aagtaccgac cctaatactt tggaatacga taacattgta
     1321 tgtgcttttc acgcaacaat tttaaaggaa agtaacggaa caattaaagt ttggggcgat
     1381 gaagcgcatg ctaatggttc tacacatgtt ttagctcctg tggaagttac gcctgcaaat
     1441 gggtataatt atactggaac ccctctgcgt gtaacagcag gaagttatgc tggatctaca
     1501 ttatcatccg ttgttcacca atttgctttg ttaaccacaa caggtttgca tatctggggc
     1561 gtgccaggga cattaataaa tacaggaatt aaaaacacca atgcttttgg accaattaca
     1621 gttaatggaa atccaacagg tttgccaact ggagtgtcac cagccaacgt aaaaatgcta
     1681 tttggtgcat acagaacatt ggccattgtt acagatgatg gacatgtttg ggtattatca
     1741 tttaatggtg aaaaaaatgg cgatggaact tctcaaagtg cttccaacaa catcatttgg
     1801 caccaagtgt gtacatcttc tggaatcaca cttaaacaag tggttgcaat gcgtggaaat
     1861 accaacgcct taatggctct ttcatccgat ggaaaggtgt atacatgggg ttctaatacc
     1921 tatattaata gtggtggacc tgcgaataga acgtatgcaa ccgaaataac tttaccatca
     1981 ggattaactc caaaaatgat agggatggca ccaaatggaa
//
LOCUS       DAOQVQ010000453         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_71514_length_2016_cov_2.665987, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000453 DAOQVQ010000000
VERSION     DAOQVQ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71514_length_2016_cov_2.665987"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2016)
                     /gene="dnaE"
                     /locus_tag="PKN22_10785"
     CDS             complement(<1..>2016)
                     /gene="dnaE"
                     /locus_tag="PKN22_10785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNS43234.1"
                     /translation="KLPTLTELHQFLFNVPFAEAHNATADVEATTRCFFELIRKEVFS
                     LDELQQAPTYFEEFQTANPSVIQSIGLKHINLKAESEKLKPKTSGDEALTNVDEARTR
                     LANVSFSHLHNHTQFTVLQSTATYKDIVKKAIEYNMPAVALTDNGNMMGAFLFESEIA
                     SHNKKVKEKQKEAEENGEPFEPNFIKPIIGCEFNVCKDLTNKSSQDNGYQVVLLAKNK
                     KGYNNLIKLASIAYTEGYYYVPRIDKTVIEKYKEDIICLTGNLYGEVPNLILNVGEKQ
                     AEEALLWWKEQFQNDLYIEVMRHGQNDENRVNETLVKFSKTHNIKLVATNNTFYTSKS
                     DATAHDVLLCVKDSALVSTPKGRGRGFRFGLDNEEYYFKSQEEMKELFLDLPEAIESA
                     QEIVDKCEAYGLARDILLPAFDIPEEFQDPQDLQDNGKRGENNYLRHLTYIGAQKRYG
                     EITDEIRERLDFELATIENTGYPGYFLIVQDFCQAARDMGVSVGPGRGSAAGSAVAYC
                     IGITNVDPIKYDLLFERFLNPERVSMPDIDIDFDDEGRGDVINWVINKYGSNQVAQII
                     TYGTMAAKSSIRDTARVLDLPLSEADKIAKLVPDTSLSNIFKLDEQALSAKLKNNNDD
                     IARANELKSIGQRNDLHGTVVKQAVQLEGSLRNTGIHACGVIITQRT"
BASE COUNT          555 a          421 c          378 g          662 t
ORIGIN      
        1 aggttctttg cgtaatgatg acaccgcagg cgtgaattcc ggtatttcgc aaagaacctt
       61 ccaattgaac agcttgttta actactgttc catgtaaatc attgcgttgt ccaatacttt
      121 tgagttcatt agctcgcgca atgtcatcat tgttgttttt tagttttgca gataacgctt
      181 gttcatccaa cttaaagata ttggaaagcg acgtgtctgg aactaatttc gctattttat
      241 cagcttccga aagtggtaaa tccaacacac gagccgtatc acgaatggat gatttagcag
      301 ccatcgtacc gtaggtaatg atttgagcca cctgattaga accgtattta ttgattaccc
      361 agttaatgac atcgccacga ccttcatcgt caaagtcaat atcaatatcg ggcatcgaaa
      421 cacgctctgg attcaagaaa cgctcaaaaa ggaggtcgta tttgataggg tcaacattgg
      481 taatcccgat acaataggca acagcagacc ccgcagcaga cccacggcct ggtccaaccg
      541 aaacgcccat atcacgtgct gcttgacaaa aatcttgtac aatcaaaaag taacctggat
      601 atcctgtgtt ttcaattgtt gccaactcaa aatccaagcg ttctctaatt tcatcggtaa
      661 tttcaccata tcttttttga gcaccaatat aggtaaggtg acgcaagtaa ttattctcac
      721 ctctttttcc attgtcttgc aaatcttgtg gatcttgaaa ttcttcagga atgtcaaatg
      781 ctgggagcaa aatatcacgc gccaagccat acgcttcaca tttatctaca atttcttgcg
      841 ccgattcaat ggcttcgggc aaatccaaga atagttcttt catttcttct tgcgacttga
      901 aataatattc ttcgttgtct aaaccaaaac ggaatcctct accacgacct tttggggtac
      961 tcaccaaagc agaatcttta acacataaca acacatcgtg agcagtagca tccgatttgg
     1021 aggtgtaaaa cgtgttgttg gtagccacca atttaatgtt gtgcgtttta gagaatttta
     1081 ccaaggtttc gttcacacgg ttttcgtcgt tttgcccgtg acgcatcacc tctatgtata
     1141 aatcgttttg aaattgctct ttccaccaca acaatgcttc ttcggcttgt ttttcaccca
     1201 cattaaggat gaggttggga acttctccgt ataaatttcc cgtcaagcaa atgatgtctt
     1261 ctttgtattt ttcaataacc gttttatcaa tgcgaggaac gtaataatag ccttctgtat
     1321 aggcgataga tgccaattta attaagttgt tgtagccttt tttgtttttt gccaacaaaa
     1381 caacttgata accgttatct tggctggatt tattggttaa atctttacac acattgaatt
     1441 cacatccaat aattggcttg ataaaattgg gttcaaaagg ttcaccattt tcttcggctt
     1501 ctttttgctt ttcttttact tttttattgt ggcttgcaat ttcactttca aacaaaaaag
     1561 cgcccatcat gtttccgttg tcggttagag caacagctgg catgttgtat tcaatggctt
     1621 tttttacaat gtctttatag gttgccgttg attgaagaac agtaaattgt gtgtggttgt
     1681 gcaaatgcga aaaagaaaca tttgccaaac gggtgcgagc ttcatctaca ttggttaacg
     1741 cttcatctcc cgaagtttta ggttttaatt tctcactttc cgcctttagg ttaatatgct
     1801 ttaaaccaat ggattgtatt acagatgggt tggctgtttg aaattcctca aaataagtgg
     1861 gtgcttgttg caattcatcc aacgaaaaca cttcttttcg aatcaactca aagaaacagc
     1921 gagttgttgc ctctacgtcg gctgttgcat tgtgcgcttc agcaaacgga acgttgaata
     1981 gaaactggtg caattccgtt aatgtaggca atttga
//
LOCUS       DAOQVQ010000454         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_71559_length_2015_cov_3.182653, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000454 DAOQVQ010000000
VERSION     DAOQVQ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71559_length_2015_cov_3.182653"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKN22_10790"
     CDS             <1..636
                     /locus_tag="PKN22_10790"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNS43235.1"
                     /translation="YHPSQPIEFPHSVHAGINGIDCRYCHNVVTESKSASLPTVNVCM
                     NCHKQVSGTTPEQQEKIKKIYEAAGWDGNQFTGKTKEIVWNKVHVLPDHVFFSHEQHV
                     TAGGLDCIQCHGDMKKRKETAQVMPVAELNKIEGNIQLTKPTLTMGWCIECHDQKGIS
                     EGPLDTKKDGYYAEIHKRLLKNDPKLYEQYMEDGKVSVKELGGWECAKCHY"
     gene            674..>2015
                     /locus_tag="PKN22_10795"
     CDS             674..>2015
                     /locus_tag="PKN22_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNS43236.1"
                     /translation="MGTTKKYWKGIEELHETPEFLQSKQQEFAGYQTIEQFLGSEEIE
                     KTSTNRRDFLKFLGFSVAAATVAACESPVTKIIPYVNKPENVTPGTATWYASTYYDGD
                     SYGSILVKTREGRPIYIKGNREQGISLGGVNAQMIASVLGLYEASRLTDALDGGKVAK
                     LSAVDGNIMKAVRSANKVTLFSNTVISPSLNKAIAQFAAKLGEGKFEHIQYDAQSYAG
                     MRAANKESFGLVVEGTDNGIIPDYDFSKAKTVVSVGADFLNSWLLSTKFSTQFGLTRN
                     PDNDWMSKLFVFEAQMSITGSNADYRGMIKPSEQANVLAYILKAFGVTTDVDTNLPAQ
                     AKTIADLAVKELKASKAESLVVSGSNNKAVQILTNKLNEVLGAYTKTINVNNPINLFK
                     SEDAKVQQFVSDVIAGKANDVVIFLDTNPVYSLPNADEFGKAISKIKTSVAISQF"
BASE COUNT          705 a          324 c          400 g          586 t
ORIGIN      
        1 tatcatcctt cacaaccaat tgaattcccg cactcagttc acgctggaat taacggaatt
       61 gattgtagat attgtcacaa cgttgttaca gaatcaaaat ctgcaagttt accaactgta
      121 aacgtgtgta tgaactgtca caaacaagtg tctggaacaa cacctgagca acaagaaaaa
      181 atcaaaaaga tttacgaagc tgctggttgg gacggaaatc aatttacagg aaaaacaaaa
      241 gaaattgttt ggaacaaagt tcacgttttg cctgatcacg ttttctttag ccatgagcaa
      301 cacgtaacag ctggtgggtt ggattgtatc caatgtcacg gtgatatgaa aaagagaaaa
      361 gaaactgcac aagtaatgcc agttgcagag ttgaataaaa ttgaaggaaa tattcaatta
      421 actaaaccta ctttaactat gggttggtgt attgagtgtc acgatcaaaa aggaatttct
      481 gaaggtccgt tagatactaa aaaagacgga tattatgcgg aaatccataa gcgtttgctt
      541 aaaaacgacc ctaaattgta tgaacaatac atggaagacg gaaaagtttc tgtgaaagaa
      601 ttaggtggtt gggaatgcgc taagtgtcac tattaattaa gtattaacga aattttagaa
      661 gtcgaaatag aacatgggaa cgacaaaaaa atattggaaa ggaattgaag aacttcatga
      721 aacacctgag tttcttcaat caaaacaaca agagtttgcg ggttaccaaa caattgaaca
      781 gtttttaggt agtgaagaaa ttgagaaaac ttctacaaac cgtagagatt tcttgaaatt
      841 cttaggattc agcgttgctg ctgctacagt tgcagcttgt gaatcacctg ttacaaaaat
      901 tattccttac gttaacaaac cagaaaacgt aactccaggt actgcaacat ggtatgcatc
      961 tacctattac gatggtgatt cttatggtag catcttggtg aaaacgcgtg aaggtcgtcc
     1021 aatttacatc aaaggaaata gagagcaagg tatatcttta ggaggtgtta atgcacaaat
     1081 gattgcatct gttcttggat tatacgaagc ttctcgttta acggatgcgt tggatggtgg
     1141 aaaagttgct aaattatctg ctgttgacgg taacatcatg aaagctgtaa gaagcgctaa
     1201 taaagttact ttgttttcaa atacagtaat tagcccttca ttaaataaag caattgcaca
     1261 atttgcagct aaattaggag agggtaaatt tgagcacatt caatacgacg cgcagtctta
     1321 tgctggtatg cgtgctgcta acaaagaatc ttttggttta gtagttgaag gaactgataa
     1381 tggtatcatc cctgattatg acttctcaaa agctaaaaca gtagtttcag ttggagctga
     1441 cttcttgaac tcatggttgc tttctacaaa gttctctact caatttggtt tgactagaaa
     1501 tccagataac gattggatgt ctaaattgtt tgtgtttgaa gctcaaatgt caatcacagg
     1561 ttcaaatgca gattacagag gaatgattaa accttctgag caagcaaatg tgttggctta
     1621 catcttgaaa gcatttgggg taacaactga cgtagatact aatttaccag cacaagcgaa
     1681 aacaattgct gatctagctg ttaaagaatt gaaagcgtct aaagcagaat cattagttgt
     1741 ttctggttca aataataaag ctgttcaaat tcttaccaat aaattaaacg aagttttagg
     1801 agcttacaca aaaacaatca atgtaaacaa tcctatcaac ttgtttaaat ctgaagatgc
     1861 aaaagttcaa caatttgtta gtgatgtaat cgctggtaaa gcaaatgatg ttgttatctt
     1921 cttagataca aacccagttt attcattgcc aaatgctgac gaatttggaa aagcaatttc
     1981 taaaatcaaa acatcggtag cgatttctca atttg
//
LOCUS       DAOQVQ010000455         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_71796_length_2011_cov_3.426892, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000455 DAOQVQ010000000
VERSION     DAOQVQ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71796_length_2011_cov_3.426892"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PKN22_10800"
     CDS             complement(<1..1133)
                     /locus_tag="PKN22_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNS43237.1"
                     /translation="MRFFEKIPIYLRNFIQQMLFVWVAMSFTRGLFHLLNSQAFSNVT
                     FYDYFIGAWFDLITLSLIFLPYALIYLLPFSPTIRQAKWYRFSCSFLFVTITLLAIGL
                     NLMDIEYFKYTSKRSTADLLAILTAGSDLKQLITTFITDFWYIILIFILLVIGVVKLY
                     FRWSNEKQTQHQPLWKGIMVYLLALATMVFIGRGGIVFKPVGVLDVSRFVQSSKTALV
                     LNTPFTMLKSIGSEGLEKVNYYPSIAATERYFNPIKTSHPQGILPKNTNVVVLILESF
                     GKEFIGYYTREKSYTPFLDSLLGESLVFDAGFANGKKSIEAVPSIFASIPSLQDNPYI
                     SSQYNSNRIVGLPELLKKHGYESAFYHGATNGSMRFDAFADYIG"
     gene            complement(1141..1707)
                     /gene="lpcA"
                     /locus_tag="PKN22_10805"
     CDS             complement(1141..1707)
                     /gene="lpcA"
                     /locus_tag="PKN22_10805"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNS43238.1"
                     /translation="MSKQIADHFFEATAILQKFNTEENFQKIAKAGQIMVDAVNNNGK
                     IISCGNGGSMCDAMHFAEELTGRYRNDRPAIPAISISDPSHISCVGNDYGYEFVFSRY
                     VEAVGQKGDVLLAISTSGNSKNVLRAIEVAKEKGMKIVGLTGKDGGKMAELCDVEIRA
                     PHSEYADRAQEIHIKCIHSLIDYIERNK"
     gene            1818..>2011
                     /locus_tag="PKN22_10810"
     CDS             1818..>2011
                     /locus_tag="PKN22_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43239.1"
                     /translation="MKKTILIALSIFSFSTLFAQEGCFDKLQKAFEKRGSYAVADDMH
                     RNVILSFFTPDGQECLSGKVR"
BASE COUNT          626 a          369 c          412 g          604 t
ORIGIN      
        1 ccaatgtagt ctgcaaaggc atcaaaacgc atggagccgt tggtagctcc gtgataaaac
       61 gccgattcgt agccgtgttt tttcaacagt tcgggtaaac ctacaatgcg attggagttg
      121 tattgcgagg aaatataagg attgtcttgc aagctgggaa tggatgcaaa aatagacggc
      181 actgcttcga tggatttttt tccgttggca aaaccagcat caaacactaa ggattctcct
      241 aacaaggaat ctaaaaatgg tgtataggat ttttctctgg tgtaatatcc aataaattct
      301 ttgccaaacg attctaaaat gagcaccacc acattggtat ttttgggtaa aatgccttgc
      361 gggtgactgg tttttatggg attaaaatag cgttcggttg ccgcaatgga aggatagtaa
      421 ttcacttttt ctaatccttc actaccaatg gatttgagca tggtaaacgg cgtattgagc
      481 accaaagcgg ttttggaaga ttgcacaaaa cgcgaaacat cgagtacgcc aacaggttta
      541 aaaacaatgc ctccgcgacc aataaacacc atcgtagcaa gtgctagaag atacaccatt
      601 attcctttcc aaagcggttg atgttgggtt tgtttttcat tgctccagcg gaaatacagt
      661 tttacaactc caatcaccaa tagaatgaaa ataagaatga tgtaccaaaa atcggtgata
      721 aaggtggtaa ttaattgttt taagtcggag cctgcggtga gaatggcgag caaatcggcg
      781 gtggaacgtt tgctggtgta tttaaaatat tcgatgtcca ttaaattgag tccgatggcc
      841 aacaaagtaa tggtcacaaa taaaaatgaa caagaaaagc ggtaccattt agcttggcgt
      901 atggtaggcg aaaacggtaa taaatagatt aaggcatagg gtaaaaatat caaactgagg
      961 gtgattaaat caaaccaagc gcctataaaa taatcgtaaa aagtaacgtt tgaaaaagcc
     1021 tgactattga gtaggtgaaa caatcctcgt gtgaaggaca ttgccaccca gacaaacagc
     1081 atttgttgga tgaaattgcg taaataaata ggaattttct caaaaaaacg catggagtgc
     1141 ttatttgttt cgttcgatgt aatcaattaa gctgtgaatg catttaatat gaatttcttg
     1201 tgctctatcg gcatattctg aatggggtgc tctaatttct acatcgcaaa gttccgccat
     1261 ttttccaccg tctttcccgg ttaaaccaac gattttcatt cctttttctt tggctacttc
     1321 aatggcgcga agtacgtttt ttgagtttcc cgaagttgaa attgccaaca acacatcgcc
     1381 tttttgtccc actgcctcca catagcgcga aaacacaaat tcataaccgt aatcgttgcc
     1441 tacgcaagag atatgacttg ggtcggagat ggaaatagca ggaattgcag gtcgatcatt
     1501 gcggtaacgt ccggttaatt cttctgcaaa atgcatggca tcgcacatag agccaccgtt
     1561 gccacaacta ataatttttc cgttattatt aactgcatcc accatgattt gtccggcttt
     1621 tgcgattttt tgaaagtttt cttcggtgtt aaatttttgc aagatggctg ttgcttcaaa
     1681 aaaatggtct gctatttgtt tactcattgt cgtcttgttt tgattcaaaa gtaaaatttt
     1741 atttctgaat gtaacaaaat taaggcaaac ttattatatt tgaaactcgg atttttaatc
     1801 cactaactaa aattaatatg aagaaaacaa tacttatcgc ccttagcatt ttcagtttta
     1861 gcacattatt tgcgcaagaa ggctgttttg acaaactaca aaaagctttt gaaaaacgcg
     1921 gttcttatgc cgttgctgac gatatgcacc gcaatgtaat tttaagtttt ttcacaccag
     1981 acggacaaga gtgtttaagt ggaaaagttc g
//
LOCUS       DAOQVQ010000456         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_72056_length_2007_cov_3.183402, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000456 DAOQVQ010000000
VERSION     DAOQVQ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72056_length_2007_cov_3.183402"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="PKN22_10815"
     CDS             complement(<1..123)
                     /locus_tag="PKN22_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNS43240.1"
                     /translation="MNFEELKEFLDFKAELYENSDFISSDPIQIPHSFSKKEDIE"
     gene            complement(123..776)
                     /locus_tag="PKN22_10820"
     CDS             complement(123..776)
                     /locus_tag="PKN22_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS43241.1"
                     /translation="MLVAKNIHKRYDDLEILKGIDLEINKSEVVSIVGSSGAGKTTLL
                     QILGTLDTPNSGTLEINGINPFSLSTKKMAEFRNQHIGFIFQFHQLLPEFTAIENVCI
                     PAMINGKSMSEAKKLALPLLERLGLSHRAEHKPNELSGGEQQRVAVARALVNNPSIIF
                     ADEPSGNLDSENSTSLHQLFFDLRNEFQQTFVIVTHNQELAKMADRTITMRDGKISS"
     gene            complement(777..2000)
                     /locus_tag="PKN22_10825"
     CDS             complement(777..2000)
                     /locus_tag="PKN22_10825"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNS43242.1"
                     /translation="MSTVLNTIEEAIEDLRKGKVIIVVDDEDRENEGDFIAAAECVTP
                     EMINFMATHGRGLICAPLTGERCDELGLTQMVNNNTVLHETQFTVSVDLIGNGCTTGI
                     SVHDRAKTIKALVDSNTKPSDLGRPGHIFPLRAKDGGVLRRTGHTEASVDLARLAGFS
                     PAGILVEILNEDGTMARLPQLMEVAKKFDLKLISIEQLIKYRMHHESLIEREVEVEMP
                     TKLGEFRMVAYKEKLTDQDHLALIKGEWKEDEPILVRVHSSCMTGDIFGSCRCDCGPQ
                     LEKSMEMIQKEGKGVIVYMNQEGRGIGLLNKLKAYKLQEEGLDTVEANLHLGFKGDQR
                     DYGVGAQILRDLGVTKMRLMTNNPTKRTGLVGYGLEIVENVPIEVESNQYNEFYLQTK
                     KDKMGHTLKLIKKHS"
BASE COUNT          520 a          417 c          378 g          692 t
ORIGIN      
        1 ttcgatgtct tcttttttgc taaaagagtg agggatttga attgggtcgg agctaataaa
       61 atccgagttt tcgtataatt ccgctttaaa atcaagaaat tctttgagtt cttcgaagtt
      121 catcaactgc taattttccc atcgcgcatg gtgatggtgc ggtctgccat tttggctaat
      181 tcttggttgt gtgtcacgat gacaaaggtt tgttgaaact cattgcgcaa gtcaaaaaac
      241 aattggtgca acgatgttga gttttcagaa tccaaatttc ctgatggttc gtcggcaaat
      301 ataatgcttg gattgttgac caatgcacgg gcaacggcaa cacgttgttg ttctcctccc
      361 gaaagttcgt tgggtttgtg ttctgcgcgg tgactcaaac ctaaacgttc taagagtggc
      421 aatgcaagtt tttttgcttc ggacattgat tttccgttaa tcatagcagg aatacacacg
      481 ttttcaatgg ctgtaaactc aggcaataac tgatggaatt gaaaaataaa tccgatgtgt
      541 tggttgcgaa attcggccat tttttttgtg gaaagcgaaa acggattgat gccgttaatt
      601 tccaacgtac cactattggg tgtatccagt gttcctaaaa tttgaagcaa agtagttttt
      661 ccagcacccg atgaacctac aatggaaacc acttcacttt tgttgatttc caaatcaata
      721 ccttttagta tttccagatc atcatagcgc ttgtgaatgt tctttgctac taacatttac
      781 gaatgttttt tgattaattt taaggtatgt cccattttat cttttttggt ttgaagataa
      841 aactcgttgt attggttgct ttccacttcg attggaacat tttcaacaat ttcaaggccg
      901 tatcctacca aaccagttcg ttttgttggg ttgttggtca ttaagcgcat ttttgttacg
      961 cctaaatcac gcaaaatttg tgcacccacg ccgtaatcgc gttgatcgcc tttaaatcca
     1021 agatgtaaat tagcttcaac ggtatccaat ccttcttctt gcaatttgta ggcttttaat
     1081 ttattgagca atccaattcc tcttccttct tgattcatgt aaacaataac gcctttgcct
     1141 tctttttgaa tcatctccat cgatttttca agttgaggac cgcaatcgca acggcaagaa
     1201 ccaaaaatat cgccggtcat gcacgaagag tgaacacgca ctaaaatcgg ttcatcttct
     1261 ttccattcac ctttaatcaa cgccaagtgg tcttgatcgg ttaatttttc tttataggcc
     1321 accattctaa attcacccaa ttttgtaggc atttccactt ctacttcacg ttcaattaac
     1381 gattcgtggt gcatacggta tttgattaat tgttcgatgg aaattaattt taaatcaaat
     1441 tttttggcaa cttccattaa ttgtggaagg cgcgccatag ttccgtcttc gtttaatatt
     1501 tcaactaaaa taccagctgg agaaaagcct gcaagtcgag ccaaatctac cgaagcttcg
     1561 gtgtgtcccg ttcttcttaa aacacctcca tcttttgcac gaagtgggaa aatatgccca
     1621 ggtcttccta aatcacttgg tttggtgttg gaatctacca atgctttgat tgtttttgct
     1681 ctatcgtgaa cggaaatacc tgttgtgcaa ccgtttccaa ttaagtctac cgacactgta
     1741 aattgcgttt cgtgcaatac ggtgttgttg ttcaccattt gtgtcaatcc caattcgtcg
     1801 caacgctcac ccgttagagg agcgcaaatc aatccacgtc cgtgtgtggc catgaagttg
     1861 atcatttcgg gagttacaca ttctgctgca gcaataaaat caccttcgtt ttcacggtct
     1921 tcatcatcta caacaatgat tacttttcct ttgcgtaggt cttctattgc ttcttctatg
     1981 gtgtttaaaa cagttgacat attcttt
//
LOCUS       DAOQVQ010000457         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_72650_length_1996_cov_3.624936, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000457 DAOQVQ010000000
VERSION     DAOQVQ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72650_length_1996_cov_3.624936"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKN22_10830"
     CDS             <1..205
                     /locus_tag="PKN22_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNS43243.1"
                     /translation="YFLLEYFSLKEMLTFYEQKYRDSNFFAVLKSLNYFEDAEEQAEP
                     KMYKMFNWEEAKKRIQHELTMLL"
     gene            294..1463
                     /locus_tag="PKN22_10835"
     CDS             294..1463
                     /locus_tag="PKN22_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43244.1"
                     /translation="MSKNSFIFVVCGAKEHIDTLHFSLKYLQKYSKNEIWVLTDSSRN
                     ETPVLHDKIVDVQTPQEYNHHQASIFLKTGIFNYFPKGNRYCYLDTDIIACSEEVDAI
                     FDQYQAPITFAPDHCTMEQFSAYAVNCDCIKTYNHYTDLLENHLVKIDPLRTSTQPMI
                     VENRKKLVAYYQKHTSVFQKIQLGLRFLLSFKQFKISDGLYFDKKERVWKDANAQVFM
                     NHFRWPSVSKKVGLKWNYIKGYPILPDGRSLWQLSCTHLPQYIHEKFNTTVQNNRFQH
                     WNGGVFLFDDQSHDFLNTWFEKTMEIFKDPKWKTRDQGTLIATIWKKGLQHHPMLSKK
                     WNLIADFHNPHLKWVNNEIQLAENERYQPAFVHVYHHFGDEHWNFWNELIEKVNR"
     gene            1495..>1996
                     /locus_tag="PKN22_10840"
     CDS             1495..>1996
                     /locus_tag="PKN22_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide-diphospho-sugar transferase"
                     /protein_id="HNS43245.1"
                     /translation="MKTALCTISTQSHLFKSFALLDAVAQYSTTDLCCLVTDANHHQL
                     KDGVVFHHLNDLTSNLAQTIQQKYTNDKLRWSLKPVYVQYLLEQGYDRVIYVDNDIFF
                     FDSPDFLFEKLKTSDFLLTPHFYPANPQKEQNWLEANFRVGLYNAGFFGATKNAIPIL
                     EWWAKCC"
BASE COUNT          682 a          347 c          334 g          633 t
ORIGIN      
        1 atattttctt ttagaatatt ttagcttgaa agaaatgtta actttttatg agcaaaaata
       61 ccgtgatagt aacttttttg ctgttttgaa aagtttaaat tattttgaag atgctgaaga
      121 acaagctgaa ccaaaaatgt ataaaatgtt taattgggaa gaagcaaaaa agagaattca
      181 gcatgaattg actatgttac tttgatgaaa aataataaat caaatcttag aaatttttta
      241 aaataccttg caaattagga atctgattcc taaatttcat aaaatacaaa aaagtgagta
      301 aaaatagctt catattcgtt gtttgtggtg caaaagaaca cattgataca cttcatttct
      361 cgttgaaata ccttcaaaag tattctaaaa atgaaatttg ggtgttgaca gattcttcac
      421 gcaatgaaac tccggtgctt cacgacaaaa ttgttgatgt tcaaacaccc caagaataca
      481 accatcacca agcaagtatt tttctaaaaa ctggaatctt caactatttc cccaaaggaa
      541 atcgctattg ttacttggat acagatatta ttgcttgctc agaagaagta gatgcaattt
      601 ttgaccaata ccaagcacct attacctttg cacccgacca ttgcaccatg gaacaatttt
      661 cagcttatgc ggtgaattgc gattgcatta aaacatataa ccactatacc gacttgttgg
      721 aaaaccattt ggtgaaaata gacccactaa gaacttctac acaacctatg attgttgaaa
      781 acagaaaaaa attggtggct tattaccaaa aacatacttc cgtatttcaa aaaatccaac
      841 tgggattgcg ttttttattg tcattcaaac aatttaaaat ctcagacgga ctctattttg
      901 ataaaaaaga acgcgtttgg aaggatgcaa atgcacaggt atttatgaac cattttcgtt
      961 ggccttcggt tagcaaaaaa gtgggtttga aatggaatta tataaaaggt tatcccattt
     1021 tacctgacgg ccgttctttg tggcaactat cgtgtacgca tttaccgcaa tacatacacg
     1081 aaaaattcaa tactacagtg caaaacaatc gtttccaaca ttggaatggc ggcgtttttt
     1141 tgtttgacga ccaatcgcac gattttttga acacttggtt tgaaaaaaca atggaaattt
     1201 tcaaagaccc caaatggaaa acacgcgacc aaggaacgct cattgccact atttggaaaa
     1261 aaggattgca gcatcatcca atgttgagta aaaaatggaa tttaattgca gatttccaca
     1321 acccgcattt aaaatgggta aacaatgaaa ttcaattagc agaaaatgaa cgctatcaac
     1381 ctgcttttgt acatgtttat catcattttg gcgatgaaca ttggaacttt tggaatgagt
     1441 tgattgaaaa agtcaatcgt tgatttattg ttaatttttt ctatttttaa ctcaatgaaa
     1501 acagctctat gcaccatatc cacccaaagt catttgttca aaagctttgc tttgttggat
     1561 gcagtggcgc aatatagcac aaccgatttg tgttgtttgg taaccgatgc caatcaccat
     1621 caactcaaag atggtgttgt ttttcatcat ttgaacgact taacatccaa tttagcgcaa
     1681 accattcagc aaaaatatac caacgacaaa ttgcgctggt cacttaaacc tgtttacgtg
     1741 caatatttgc tagaacaagg gtatgaccgt gttatttatg tggataatga catttttttc
     1801 ttcgattcgc ccgatttttt atttgaaaaa ttaaaaacgt ctgatttttt gttaactcct
     1861 catttctatc ctgccaatcc acaaaaagaa caaaattggt tggaagcaaa tttccgtgta
     1921 ggcttgtaca atgccggttt ttttggtgca actaaaaatg ccattcctat tttggaatgg
     1981 tgggcaaaat gttgtg
//
LOCUS       DAOQVQ010000458         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_72821_length_1993_cov_3.525284, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000458 DAOQVQ010000000
VERSION     DAOQVQ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72821_length_1993_cov_3.525284"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1993)
                     /locus_tag="PKN22_10845"
     CDS             complement(<1..>1993)
                     /locus_tag="PKN22_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43246.1"
                     /translation="LLMNEGMSDTIVSAKSLLVSVAGIFSFQQQIHLKSVLLEDAICK
                     LERAKDAEFINLKFVIDYFKPKKKKRKGHLPFTVDKAHLKNVRFQFDNNNIARREAGM
                     DYQHLYISQIDLKAHDIKIRNGIITGIVDGLAANEKSGFQLDKFRAGYLIVSDHGVEL
                     KDVNIQTPYSDIKSDDFKLVYQRYQQFRSFVDSVAFDVKLNKSTTSFTDISFFAPQLM
                     GMNEKVEIEGKVSKFVKNLRVDKLVLKTGLKSEVRGTINLPDFRDLRNAFYQERIDYA
                     FIDLADLQAIELPHRAGKRYIALSENVQRFNHFEANNVSLDGIFSQFVLAADNVSTSI
                     GSVNMDNGILFTHNPANNSFLFSHSETSEYDVKVNHFNLGKFLNKPLFGEVDGLFLLS
                     GEAFSFTDIHFNKMEGNVNRFDMDGYSYNNITVKNASLVDKILYAEVDVNDDNLQLNY
                     NGTIDLNGEPKMMLQVDIKKALLGKLNYTNDLTSNLVANLVINTTGVNPNTMSGVGEV
                     NDILYVEGTKIIHVPSLSFEIDRGQNADNLSIRSSIFNADFEGKMNFKTMNYVIKDQI
                     QQLFPGVFALKPSKKINLEEHTTDDCLAFNINIIDVRSLLNIFQPNLKVANGTVINGD
                     YDVANRYFKMDLHSSEISYNNIVAKTIHWNQFADGNTIST"
BASE COUNT          652 a          380 c          289 g          672 t
ORIGIN      
        1 gtagaaattg tgtttccatc agcaaattga ttccaatgaa ttgtttttgc aacaatgttg
       61 ttatagctta tttcactgga atgtaaatcc attttaaaat aacgattagc aacatcataa
      121 tcaccattga taactgttcc gttggcaact ttaaggttgg gttgaaaaat attcaacaat
      181 gaacgcacat caatgatgtt gatattaaac gccaaacaat catcggtggt gtgttcttct
      241 aaattaattt tcttgctagg ttttagtgca aaaacaccag gaaacaattg ttgaatttga
      301 tctttaatga cataattcat tgttttgaag ttcatttttc cttcaaaatc agcattaaaa
      361 atggagctac gaatggaaag attatcggca ttttgacctc tatcaatttc aaaacttaga
      421 cttggaacat gaataatttt ggtaccttcc acatataaaa tatcgttcac ctctcctaca
      481 ccactcattg tgttgggatt aactcccgtt gtattgataa ccaagttagc caccaaatta
      541 gacgttaaat cgttggtata gtttaatttt ccgagcaatg cttttttaat atcaacttgc
      601 aacatcattt taggttcgcc attcaaatca atggttccgt tgtagttcaa ttgcaaatta
      661 tcatcgttta catctacttc ggcataaaga attttatcta ccaaagaagc atttttgact
      721 gtaatattgt tgtaggaata gccatccatg tcaaaacgat tcacgtttcc ttccatttta
      781 ttaaaatgta tgtcggtaaa cgaaaatgcc tctccactaa gtaaaaacaa gccatctact
      841 tcaccaaaca agggtttgtt taaaaattta cccaaattaa aatggtttac tttcacatcg
      901 tattcactag tttctgaatg cgaaaacaaa aacgaattat tagccggatt gtgggtaaac
      961 aaaattccat tgtccatgtt caccgaacct atagaagtag aaacattatc ggcggctaat
     1021 acaaattgcg aaaaaatacc atccaaactc acattgtttg cttcaaagtg attgaatcgt
     1081 tgcacattct cactcaatgc aatgtaccgt tttcctgcac gatgtggcaa ttctatggct
     1141 tgtaaatcag ctaagtctat aaatgcataa tcaattcgtt cttgataaaa ggcattgcgt
     1201 aaatctctaa aatcaggtag gttgattgta cctctcacct ccgattttaa tcccgttttt
     1261 agcaccaact tatcaacacg aaggttttta acaaatttgc ttaccttgcc ttcaatttca
     1321 actttttcat tcatccccat taactgaggg gcaaaaaatg aaatatcggt aaatgatgtg
     1381 gtacttttat tcaattttac atcaaaggca acactatcca caaacgagcg aaattgctga
     1441 taacgttgat ataccaattt aaaatcatcg cttttaatgt ctgaataagg tgtttggata
     1501 ttaacatcct ttaattctac tccatgatcg gaaacaatta aataacctgc ccgaaattta
     1561 tccaattgaa aacccgattt ttcattagct gctaacccat caacaattcc tgtaatgatg
     1621 ccgtttctaa ttttaatatc gtgtgctttt aaatcaattt gactgatgta taaatgctga
     1681 taatccatcc ccgcttctct gcgtgcaata ttgttgttat caaattgaaa acgaacgttt
     1741 tttaagtgtg ctttatcaac agtaaatgga aggtgccctt ttcttttctt tttcttaggt
     1801 ttaaaatagt caataacaaa tttaagattg ataaattcag catcttttgc acgttccaac
     1861 ttacaaatag catcttctaa caaaacagat tttaaatgta tttgctgttg aaaagaaaaa
     1921 ataccggcaa cactcaccaa caaacttttg gcactaacaa tggtatcgct catgccttcg
     1981 ttcatcaaca aca
//
LOCUS       DAOQVQ010000459         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_73188_length_1987_cov_2.811077, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000459 DAOQVQ010000000
VERSION     DAOQVQ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73188_length_1987_cov_2.811077"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="PKN22_10850"
     CDS             complement(<1..1502)
                     /locus_tag="PKN22_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNS43247.1"
                     /translation="MIDTDLETAKRLYPFQEKTVNSIIEELKANGTNFNLLYQLPTGG
                     GKTVIFSEIAKQYIQQWNKKVLILTHRIELSVQTSKQLSAIGVKNKIINSEVKSVPDQ
                     GEYSCFIAMVETLNNRLQDDENFIEDIGLVIVDEAHYNSFRKIFQFYQGGNILGVTAT
                     PLSSNRVLPLNDNYNKLMVGESIASLIENQYLANAETFTYDVNLHGLKIGSNGDFTIS
                     SSDVLYGNYFMQEKLLFAYEEIAIGSKTLIFNPGIETSIRVAETFKKRGFKTRHLDST
                     FSDKDRKEVLQWFKETPDAILTSVGILTTGFDEPTVETIILNRATRSLTLYHQMIGRG
                     SRKLPNKDYFKVIDLGNNVRRFGLWQDYINWQDAFRFPDRFLESRLSESDDLEFEVEF
                     EFPKKLAETIDTAKLEAFSIRDVYYECLDNGDKGKQAVDLSLENHFEVIRDASEDFYD
                     AIAIQDGLQDHIEHRLKMYTKCIAKSTNNYFKYLMETYNRQLRQKLRAEL"
     gene            1664..>1987
                     /locus_tag="PKN22_10855"
     CDS             1664..>1987
                     /locus_tag="PKN22_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43248.1"
                     /translation="MKALSTVQSVLLLLIGVVSLFMTLSIVFDLFGIREKEGNYVLFV
                     VYANLLCGTLYVATVFTNWKKNYKWSFILLALAFMVLVVTFYFFKEHIAQGGIYEPKT
                     VKAMTF"
BASE COUNT          595 a          351 c          348 g          693 t
ORIGIN      
        1 tccaattctg ctcttaactt ttgtcttaat tgcctgttgt aagtttccat caaatatttg
       61 aagtaattat tggtgctttt agcaatgcat ttggtataca ttttcaaacg gtgttcaatg
      121 tggtcttgca atccatcttg aatggcaata gcatcataaa agtcttcaga agcatctcgg
      181 atgacctcaa aatggttttc caatgacaaa tcaacggctt gtttgccttt atcgccatta
      241 tccaaacatt cgtaatacac atcgcggatg ctaaatgctt ctaatttggc tgtatcaatt
      301 gtttcggcta attttttagg gaattcaaat tccacttcaa attccaaatc atcggattcg
      361 gataagcgcg attccaagaa acgatctgga aaacggaaag catcttgcca attgatataa
      421 tcttgccata aaccaaaacg gcgtacgttg ttaccaaggt caattacttt aaaataatct
      481 ttgttaggta attttctaga accacgacca atcatctggt gataaagcgt gagtgaacgt
      541 gttgcacgat tcaaaatgat ggtttctacc gtaggttcgt caaaacctgt ggttaaaatt
      601 cctactgaag ttaaaatagc gtcgggtgtt tctttgaacc attgcagcac ttcttttctg
      661 tctttatccg aaaaagttga atccaaatga cgggttttaa aaccacgttt tttaaatgtt
      721 tctgctacac gaatggaagt ttcaattcca gggttaaaaa tcaaagtttt ggatcctata
      781 gcaatttctt cataggcaaa caataatttt tcttgcatga aataattccc atacaataca
      841 tccgaacttg aaattgtaaa atccccgttg gagccaattt tcaatccgtg caagtttaca
      901 tcataagtaa acgtttctgc atttgccaaa tattgatttt caataagcga cgcaatgctt
      961 tcacctacca ttaatttgtt gtaattatcg tttaatggca aaacacggtt gctactcaaa
     1021 ggagttgctg taacccctaa aatattgcca ccttggtaaa attgaaaaat ctttctaaaa
     1081 ctgttgtaat gtgcttcgtc cacaattacc aacccgatgt cttcaataaa attttcatcg
     1141 tcttgcaaac ggttgttcaa ggtttccacc atggcaataa aacaagaata ttctccttgg
     1201 tcgggaaccg atttaacttc cgagttgatg attttgtttt tgacacctat tgccgacaac
     1261 tgtttggaag tttgcaccga caactcaatg cggtgtgtaa gtatcaatac ttttttattc
     1321 cactgttgaa tgtattgttt agcaatttcc gaaaaaatca ctgtttttcc acctccagtg
     1381 ggcaattgat acaacaagtt gaaattcgta ccgttggctt ttaattcttc aattattgag
     1441 ttgacggtct tttcttgaaa gggatataat ctcttggctg tttctaaatc tgtatcaatc
     1501 atttttctta aatggggcta caaaattact gcctttattc tgatttagca aattattgaa
     1561 gaaagtaatg tgttaaaaca aaaaaaaacg aactaaacag acaggtgtta attcatattt
     1621 taacgggtag tgttgatact tttttatact tttaaacaaa aaaatgaaag cattaagtac
     1681 agttcaatcg gttttgttgt tgttaattgg agttgtgtcg ttgtttatga cactttccat
     1741 tgtgtttgat ttatttggaa tacgcgaaaa agaaggaaac tacgttttgt ttgtggttta
     1801 tgccaatctt ttatgtggca cactctatgt tgcaacggtt tttaccaatt ggaagaagaa
     1861 ttacaagtgg agttttatcc ttttggcgct tgcatttatg gtgttggtgg ttacgttcta
     1921 ttttttcaag gaacacattg ctcaaggtgg aatttatgaa cccaaaactg taaaagcaat
     1981 gacgttt
//
LOCUS       DAOQVQ010000460         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_74048_length_1972_cov_3.131455, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000460 DAOQVQ010000000
VERSION     DAOQVQ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74048_length_1972_cov_3.131455"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="ruvB"
                     /locus_tag="PKN22_10860"
     CDS             complement(<1..1009)
                     /gene="ruvB"
                     /locus_tag="PKN22_10860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNS43249.1"
                     /translation="MEGSFDYRDEAEKQDVDYDKVLRPKRLSDFTGQPKVVDNLSIFV
                     KAAKLRNEPLDHVLLHGPPGLGKTTLSNIIANELEVGFKVTSGPVLDKPGDLAGLLTN
                     LETGDVLFIDEIHRLSPVVEEYLYSAMEDYHIDIMIDSGPNARTVQIALNPFTLIGAT
                     TRSGLLTSPLRARFGINLRLEYYDAKTLTMIVERSAGLLGIPITEEAAYQIASRSRGT
                     PRIANALLRRVRDFAQIKGNGTIDTAISKYALDALNVDQCGLDEMDNKILHTIIDKFK
                     GGPVGLTTIATAIGENAGTLEEVYEPFLIQEGFLMRTPRGRQVTEKAYKHLGKDKGWQ
                     QG"
     gene            complement(1058..1555)
                     /locus_tag="PKN22_10865"
     CDS             complement(1058..1555)
                     /locus_tag="PKN22_10865"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNS43250.1"
                     /translation="MQNQNPEITSFQNKLKNPFLFRLLLYKMLPLASFTGVKMVDINS
                     NKCTLSVKYKFLNKNPFKTTYWAVLGMCAEMASGLLLLMHTYKLKPSVSTYVLSCDAQ
                     FVKRALGKTTFICEQGDEIVALIQQAIQTKEAQQINCKIIAYNHEGEVVAIFNFVWGV
                     KVRNK"
     gene            complement(1621..1929)
                     /locus_tag="PKN22_10870"
                     /pseudo
     CDS             complement(1621..1929)
                     /locus_tag="PKN22_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
BASE COUNT          610 a          407 c          340 g          615 t
ORIGIN      
        1 cgccttgttg ccagccttta tcttttccta ggtgtttata tgctttttcg gtaacctgac
       61 gtccacgcgg tgtgcgcatc aaaaaacctt cttgaattaa aaatggttca tacacttctt
      121 ctaaggtacc cgcattttca ccaatggcag ttgcaatggt tgttaaccca accgggccac
      181 ctttaaattt atctatgatg gtatgcaata ttttgttgtc catttcatcc aaaccacatt
      241 gatccacgtt gagggcatcc aaagcatatt tactaattgc tgtatcaatc gttccgtttc
      301 ctttgatttg ggcaaaatcg cgcacacgac gtaacaatgc gttggcaata cgaggtgttc
      361 cacgacttct acttgcaatt tgataggctg cttcttcagt aattggaata cctaacaaac
      421 cagcagaacg ttcaacaatc atggttaaag tcttggcatc gtaatattcc aaacgcaagt
      481 tgattccaaa acgtgcacgc aatggtgatg ttagcaaacc agaacgagtt gtcgccccga
      541 tgagagtaaa cgggtttaat gcaatttgaa cggtgcgtgc attaggaccc gaatcgatca
      601 taatgtcgat atggtaatct tccattgccg aatacaggta ttcttctaca acaggactta
      661 aacggtgaat ttcgtcgatg aacaacacat cgcccgtttc taaattggtg agtaagccag
      721 ccaaatctcc gggtttatcc aaaacaggcc cagaagtaac tttaaaacca acttccaatt
      781 cattggcaat aatgttagac aaagttgttt tacctaaccc tggaggtccg tgcaacaata
      841 cgtgatccaa aggttcattg cgcaatttag ctgctttcac aaaaatggaa aggttatcca
      901 ccacttttgg ttgccctgta aaatcagaca agcgttttgg acgtagaact ttatcgtaat
      961 ctacatcttg cttttctgct tcatctctat aatcgaaaga tccttccacg tataaattgt
     1021 attgtacaaa gatataaaaa catccaattg gtgctagtta cttatttcta acttttactc
     1081 cccaaacaaa attgaaaata gccaccactt cgccctcatg attataggct ataatcttac
     1141 aattaatttg ttgtgcttct ttagtttgaa ttgcttgttg aattaaagct acgatttcat
     1201 ccccttgttc acaaataaag gttgttttcc ccaaagctct tttaacaaat tgagcatcgc
     1261 acgaaagcac gtaggtggag acagaaggtt ttaatttata ggtatgcatt aataacaaca
     1321 atccacttgc catttctgca cacataccaa gtactgccca ataagttgtt ttaaatggat
     1381 ttttattaag gaatttatat tttacagaca acgtgcattt attggagttg atatctacca
     1441 ttttcacacc tgtaaacgat gctaaaggaa gcattttata caacaataaa cgaaacaaaa
     1501 aaggattctt taatttgttt tgaaaactag taatttctgg gttttgattt tgcataacaa
     1561 ttagttgaaa taaaaaaggc ttgcagttga ttgcaagcct ttaaaagtaa cgaatatttt
     1621 ttaatgttca ttaattcctc cgtccactaa ttcaccttca cgcaaaggaa cgttttgtgg
     1681 aacaaaatcc tcatcccatc ctggtttaga taaatcataa ggccaacggt gaacttcagg
     1741 caaagctccc tcccagtttc cgtggatatg ttctactggt gtagtccact ctaatgttgt
     1801 agatctccaa gggttttgag gtgctttagg tcctctgaaa atactataaa agaagttgaa
     1861 aaggaatacc aattgcccta atgcaccaac aatagcaaac aatgatataa ctaaattcaa
     1921 gttttcaatt ttcaagaaat aaagaagggt ttacaacttc cccctgcgaa at
//
LOCUS       DAOQVQ010000461         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_75253_length_1952_cov_4.105957, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000461 DAOQVQ010000000
VERSION     DAOQVQ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75253_length_1952_cov_4.105957"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..577
                     /locus_tag="PKN22_10875"
     CDS             62..577
                     /locus_tag="PKN22_10875"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNS43251.1"
                     /translation="MQVRTPKETLSVTTKVVLPNDTNTMGNLFGGQLLAWMDVIASVA
                     AHRHCKRVVVTASVNNISFNHPIKLGSIVTLEAKVSRAFTSSMEIITDVFVEDQVTGQ
                     RIKSNEGIYTFVAVDQNGGPIQVPEIKPETEEEQRRYDGALRRKELSLILAGKMSPND
                     AVQLKKLFEIE"
     gene            complement(636..791)
                     /locus_tag="PKN22_10880"
     CDS             complement(636..791)
                     /locus_tag="PKN22_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43252.1"
                     /translation="MSIFGIVLFLVFGGVAFWMLLFLLGFILPFWITLAIKEKIRPKR
                     IFEDDKQ"
     gene            complement(904..>1952)
                     /locus_tag="PKN22_10885"
     CDS             complement(904..>1952)
                     /locus_tag="PKN22_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS43253.1"
                     /translation="DFDGEDELEEHSDALDELVMKFTVRSDETTHSVYQADFTDNAKI
                     AESAEAKTNKVAILYDEWDCYKNQYKRDYAKVYPDYQVEINEKYYTDTIQKHQTLLTQ
                     LRKILANISNRRIDQRYQTHGKEIDIDMATDRFVDIVSKQTPTERVYIADKKQEKDLS
                     LLILIDISLSSDGYTDGNRVIDVAKQTAILFGEILNEYHVDFSIQCFYSQTRNNLSYI
                     TVKDFDEKWNKAKYKIGGIKPKGYTRIGGALRHSGALLHKRDHKNKWVILLSDGKPND
                     YDRYEGKYGINDVKQALRELNQLHINNYALAIESTAKYYLPQMFGQNHYQIVSSPNEL
                     IHSMIQLYDKLRVG"
BASE COUNT          593 a          350 c          377 g          632 t
ORIGIN      
        1 atgttaatga aacgcaattc aactaagtag tttcactatt tttgcaaaaa aatttaagaa
       61 aatgcaagtg cgtacaccaa aagaaacgtt gtctgtaaca acgaaggtag tgttgcccaa
      121 cgacaccaac acgatgggaa atttatttgg cggtcagtta ttggcctgga tggatgttat
      181 tgccagtgtg gcggcgcatc gccattgcaa acgcgtggta gttacagcat ctgtaaacaa
      241 tatttcgttc aatcacccga taaaattagg ttccatcgta actttagaag caaaagtatc
      301 cagagcattt acgtcatcga tggaaattat tacggatgtt tttgtagaag accaagttac
      361 gggtcaacgc attaaaagca acgagggaat ttatacgttt gtggctgtag atcaaaacgg
      421 cggtccaatt caagtgcccg aaattaaacc agaaacagaa gaagaacaac gtcgatacga
      481 tggagctttg cgaagaaaag aattgagttt aattctagca ggaaaaatga gtcccaacga
      541 tgctgttcaa ctcaagaaat tatttgaaat tgaataatgc caaaaaagcc cgattgttac
      601 aaccgggctt ttttaatgtt tatatcaaca atatattatt gtttatcgtc ttcgaaaatt
      661 ctctttgggc gaattttttc tttaattgcc aaagtaatcc aaaatggtaa gatgaatcct
      721 aaaaggaata aaagcatcca aaatgcaaca ccaccaaata ctaaaaataa aactatacca
      781 aaaatgctca taatcactct gtttgagtta taattgtcgt aaaattatat aaattatcgt
      841 ttccaccaag tcttttttta aaataaattc aaaaaaaaca ttgtttaaaa tcattttaaa
      901 tagctagcca acccttaatt tatcgtataa ctgtatcatt gaatgaatta actcattggg
      961 cgatgacaca atttgataat ggttttgacc aaacatttgt ggcaagtaat attttgcagt
     1021 ggattcaatg gccaatgcgt aattattgat gtgcaattgg ttcaactcgc gcaaagcttg
     1081 ttttacatcg ttgatgccgt attttccttc gtaacggtca taatcgttgg gtttcccgtc
     1141 cgagagcaaa attacccatt tgtttttatg gtcgcgttta tggagcaaag cccctgaatg
     1201 gcggagtgcg cctccaatgc gggtgtatcc tttgggtttg atgcctccaa ttttatattt
     1261 tgctttgttc catttttcat caaaatcttt gacggtaatg taggaaagat tgttgcgtgt
     1321 ttgcgagtaa aaacattgaa tcgaaaaatc aacgtggtat tcattgagaa tttcaccaaa
     1381 taaaatggcc gtttgcttgg caacatcaat aacacggttg ccgtctgtat aaccatcact
     1441 ggataagctg atgtcaatca aaatgagcaa cgataaatct ttttcttgtt ttttatcagc
     1501 tatatacacg cgttcggtgg gtgtttgttt ggatacgatg tccacaaaac ggtcggttgc
     1561 catatcaatg tcaatttcct taccatgcgt ttggtaacgt tgatcgatgc gtcggttgct
     1621 aatgttggct aaaatttttc gcaattgcgt gagcaacgtt tggtgttttt ggatggtatc
     1681 ggtgtagtat ttttcgttta tttctacctg ataatcggga tacaccttgg cataatcacg
     1741 tttgtattga tttttgtagc aatcccattc atcgtataaa atggccactt tgttggtttt
     1801 ggcttcggca ctttcagcaa tttttgcatt gtctgtaaaa tcggcttgat atacactgtg
     1861 cgttgtttcg tcggaacgaa cagtgaattt catgactaat tcatccaatg catcgctgtg
     1921 ttcttccagt tcatcctctc catcaaaatc ac
//
LOCUS       DAOQVQ010000462         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_75450_length_1949_cov_4.159451, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000462 DAOQVQ010000000
VERSION     DAOQVQ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75450_length_1949_cov_4.159451"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKN22_10890"
     CDS             complement(<1..824)
                     /locus_tag="PKN22_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNS43254.1"
                     /translation="MKTIYDRLIEKDCKLAVIGLGYVGLPIALEFAKKIKVVGFDINQ
                     ARVDLMKQSIDPSNELEKSAFDNSDITFTANLEDLRDVNFFIVAVPTPIDDANLPDLK
                     PLLGASSTVGKVLKKGDYVVFESTVYPGCTEEDCIPVLERESGLKFNEDFKVGYSPER
                     INPGDKQHTLQNVVKVVSGCDDESLDNIAKTYELVVAAGVHRASSIKVAEAAKIIENT
                     QRDVNIALINELSIIFNRLGINTFDVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLT"
     gene            complement(805..1830)
                     /locus_tag="PKN22_10895"
     CDS             complement(805..1830)
                     /locus_tag="PKN22_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS43255.1"
                     /translation="MSKIKFAVIGAGHIGKRHAEMVKRNEEAELVALVDVRSKEECGA
                     QEFDVPFFNSIDELLNSGIEFDVANICTPNGLHAELSLKALEAKHHVVCEKPMALSKR
                     DCEDIIFKSLQVSKQVFCVMQNRYSPPSEWIKKVIEDKVLGNIFIVQLNCYWNRDSRY
                     YKPGGWKGRQDLDGGTLFTQFSHFIDIMYWLFGDIKNIQGKFADFTHKDSTDFEDSGI
                     VNFDFINGGMGCINYSTSVATQNLESSITIIGEKGSVKIGGQYMNEVEICNIEGYEMP
                     ELAPTNPGNDYGAYKGSAANHNYVIQNVVDTLKGRNTATTNALEGLKVVDIIERIYEV
                     RNENNLR"
     gene            complement(1818..>1949)
                     /locus_tag="PKN22_10900"
     CDS             complement(1818..>1949)
                     /locus_tag="PKN22_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNS43256.1"
                     /translation="ARQIGWMSEYGHRLEFNTDGVATCPESGDKYELKNGEVSKCQK"
BASE COUNT          574 a          439 c          292 g          644 t
ORIGIN      
        1 gttaaataat atgggtcaac accaatacaa tgtccaccta ccaatcctgg tctaaatggt
       61 aagaaattcc attttgtacc agcagcttcc aacacatcaa acgtattgat acccaaacga
      121 ttgaagatga tagacaattc attgattaac gcaatattca catcgcgttg agtgttttca
      181 ataatttttg cagcttcggc aactttgatt gaacttgcac ggtgtacacc agcagcaaca
      241 accaattcgt atgttttggc aatgttatcc aacgattcgt catcgcaacc agaaacaact
      301 ttcacaacat tttgcaacgt gtgttgctta tctcctggat tgatgcgctc tggcgaatat
      361 ccaactttaa aatcttcgtt gaatttcaat cccgattcac gttctaaaac tggaatacaa
      421 tcttcttccg tacatcctgg ataaacagtg gattcaaaca ccacgtaatc gccttttttc
      481 aaaacttttc ccacagttga tgaagctcct aacaatggtt ttaaatcagg taaattagca
      541 tcgtcaattg gcgttggaac tgcaacaata aagaaattca catcgcgcaa atcttctaaa
      601 ttggctgtaa acgtaatatc gctgttatca aatgctgatt tttccaactc attgcttggg
      661 tcaatgcttt gtttcattaa atccactcgc gcttgattga tgtcaaaacc aaccacttta
      721 atttttttag caaattccaa ggcaatgggt aaaccaacat agcccaaacc aataacggct
      781 aatttgcaat ctttttctat gagtctatcg taaattgttt tcatttctta cttcgtaaat
      841 tctttcgatg atatccacta cttttaaacc ttccaaagca ttggtagtag cagtatttcg
      901 tccttttaat gtatccacta cgttttgaat cacatagtta tgattggctg ccgaaccttt
      961 atatgcacca taatcgtttc ctgggtttgt tggcgccaat tcaggcattt catagccctc
     1021 gatgttgcaa atttctactt cgttcatgta ttgaccacca attttcacgc ttcctttttc
     1081 tccaataatg gttatagagc tttctaaatt ttgcgttgca acagaagttg aataattgat
     1141 gcatcccatt ccaccgttga taaaatcaaa attcacaata cctgaatctt caaaatcggt
     1201 tgaatctttg tgggtaaaat cagcaaattt tccttgaata tttttaatat caccaaacaa
     1261 ccaatacatg atgtcgataa agtgagaaaa ttgagtaaac aaagttccac catccaaatc
     1321 ttgtcttcct ttccatcccc caggtttgta ataacgacta tctctgttcc aatagcagtt
     1381 caactgaacg atgaaaatat ttccaagcac tttgtcttca atcacctttt taatccactc
     1441 agatggtggt gaatagcggt tttgcattac acaaaatact tgttttgaaa cttgcaatga
     1501 tttgaagata atgtcttcac aatcgcgttt actcaatgcc attggttttt cgcacactac
     1561 gtggtgtttt gcctccaatg cttttaaact caattctgca tgcaaaccgt tgggcgtaca
     1621 aatattggca acatcaaact caattccaga gttcaacaac tcatcaatgg aattaaaaaa
     1681 gggtacatca aattcttgag caccacattc ttcttttgaa cgcacatcca ccaaagcaac
     1741 caattcggct tcttcgttgc gtttcaccat ttcagcatga cgtttcccga tgtgtcctgc
     1801 tccaataact gcaaatttta tttttgacat tttgatactt ctccgttttt caattcgtat
     1861 ttatctccac tttctggaca agtagccaca ccatctgtgt taaactctaa acggtgaccg
     1921 tattcactca tccatccaat ttggcgtgc
//
LOCUS       DAOQVQ010000463         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_75587_length_1947_cov_3.427061, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000463 DAOQVQ010000000
VERSION     DAOQVQ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75587_length_1947_cov_3.427061"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKN22_10905"
     CDS             <1..320
                     /locus_tag="PKN22_10905"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNS43257.1"
                     /translation="FMSKIADLQSNNIKNIILDPGFGFSKTMEENHYLLHHLDAFKTF
                     NLPILVGVSRKSMIYKKLGISPENALNGTTALHTIALMKGASILRVHDVQPCKEVIDL
                     IYR"
     gene            complement(317..1444)
                     /locus_tag="PKN22_10910"
     CDS             complement(317..1444)
                     /locus_tag="PKN22_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNS43258.1"
                     /translation="MESPIYPFSEEILPGAFQMQQYLPLLANKRVAIVANQASVVGEQ
                     HLVDTLLSRQVNIVKVFSPEHGFRGDNDAGASVNDTIDNLTKLPIVSLYGNNKKPKPT
                     DLDSIDVILFDLQDVGVRFYTYISTLHYIMEAAAESNIPVILLDRPNPNANIIDGPVK
                     KPGFESFISMHPVPVLYGMTIGEYGQMINGEYWLKDSVQCELTVIELLNYNHKRRYSL
                     PIPPSPNLRTDNAIMLYPSLCFFEGTVVSVGRGTDLPFELYGHPLFPDEGFAFIPSSK
                     IGASNPVLKDQFCYGVDLTEIKHRFKRLRLDYLLHAHDILFPILGEKWIDRPRFFNLL
                     AGSDELLKQINACASESEIRESWKSDLKAFKLVRAKYLIYD"
     gene            1554..>1947
                     /locus_tag="PKN22_10915"
     CDS             1554..>1947
                     /locus_tag="PKN22_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43259.1"
                     /translation="MSFEYFISKRILKNEVQGKKVSKAIVRISVISIALAIVVNLITI
                     AIVKGFQSQVRDKVSGFGAHLNILSASEGNMYESSPILKNQAFLKDVEKIKGIKSISS
                     VGYKPVLFQSDKSERVVKLLNNKDTLIKE"
BASE COUNT          645 a          339 c          354 g          609 t
ORIGIN      
        1 attttatgtc aaaaatcgct gatttacaat caaataacat taaaaacatc atccttgacc
       61 ctggatttgg atttagcaaa acgatggaag aaaatcatta tttattacac cacttggatg
      121 catttaaaac attcaattta ccaattttgg ttggtgtttc tcgtaaatca atgatttata
      181 aaaaacttgg aatttctcca gaaaatgctt tgaatggtac aacagcactt catactattg
      241 cattgatgaa aggcgcttca attttaaggg tacacgatgt tcaaccgtgt aaagaagtga
      301 ttgatttgat ctatcgttaa tcgtagatta aatattttgc acgaaccaat ttgaatgctt
      361 ttaagtcgct tttccaactt tcacgaattt cggattcact tgcacatgcg ttgatttgct
      421 tcaacaattc atcacttcca gctaataaat taaaaaatcg aggtcgatcg atccattttt
      481 cccctaaaat aggaaacaaa atatcgtggg catggagcaa ataatccaat cgcagacgtt
      541 taaatcgatg ttttatttct gttaaatcaa cgccataaca aaactgatct tttaaaacag
      601 ggtttgaggc accaatttta gaagatggga taaatgcaaa tccttcatct ggaaataatg
      661 ggtgaccata aagttcaaat gggaggtcgg ttccacgtcc aacacttaca accgtacctt
      721 caaaaaaaca caaacttgga tacaacatga ttgcattatc ggtacgcaag ttgggggatg
      781 gagggatggg aagagaatac ctgcgtttat ggttgtaatt caatagttcg ataactgtta
      841 actcacattg cacgctgtct ttgagccaat attcaccatt aatcatttgt ccatactcgc
      901 caatggtcat tccataaagc actggaacgg gatgcatgct gataaatgat tcaaaaccag
      961 gttttttaac tgggccatct attatatttg cattaggatt gggtctatcc agcaaaatga
     1021 caggaatatt gctttctgca gctgcttcca tgatgtaatg caaagtagaa atataggtat
     1081 agaaacgaac ccctacgtct tgtaaatcaa ataaaatgac atcaatgctg tctaaatcgg
     1141 ttggttttgg ttttttattg ttgccgtaga gcgatacaat tggtagtttt gttaaattgt
     1201 caattgtatc gttgacactt gctcctgcat cgttgtctcc acgaaaacca tgttcaggag
     1261 aaaaaacttt gacgatattt acttgtcttg acaagagcgt gtcaaccaaa tgttgttcac
     1321 ctacaacaga tgcttggttg gcaacaatgg ccactctttt attggcaagc aaaggaagat
     1381 attgctgcat ttgaaaagca ccaggaagaa tttcctcgct aaaaggatat ataggagatt
     1441 ccaatttatt ggcaaatgaa accgatgtat ttgttataat tagaattaaa aacaggcaaa
     1501 ttctttttaa gccaaaatcc tttatgtatt ttagtgccca aaattgttgt ctattgtcat
     1561 tcgaatattt catatcaaag cgtattctta aaaacgaggt gcaaggtaag aaagtttcca
     1621 aagccattgt gcgtatatct gtgataagta ttgcactagc gattgttgtt aacttaatta
     1681 caattgccat cgttaaagga tttcaaagtc aagttcgtga caaagtttct ggtttcgggg
     1741 cgcatttaaa catattatcg gcatccgaag gaaacatgta cgaatcttca cccatcctca
     1801 aaaatcaagc ctttttaaaa gatgttgaaa aaataaaggg aataaaaagc atttcttctg
     1861 ttggatacaa gcctgttttg tttcaaagtg ataagagtga acgcgttgta aaattgctca
     1921 ataacaaaga caccttgata aaagaac
//
LOCUS       DAOQVQ010000464         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_76225_length_1937_cov_3.149309, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000464 DAOQVQ010000000
VERSION     DAOQVQ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76225_length_1937_cov_3.149309"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1405)
                     /locus_tag="PKN22_10920"
     CDS             complement(95..1405)
                     /locus_tag="PKN22_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNS43260.1"
                     /translation="MKIYNNILETIGNTPLVRLNKITKDVKATVLAKVETSNPGNSVK
                     DRMAVKMIEDAEKAGLIKPGGTIIEGTSGNTGMGLALACIIKGYKLICVLNDKQSKEK
                     MDILRAVGAEVVVCPTAVDPSDPRSYYSVSRRLAAETPNSWYVNQYDNLSNRQAHYES
                     TGPEIWEQTDGKITHFVVGVGTGGTISGVGKYLKEKNPNIKIWGIDTYGSVFKKYKET
                     GIFDESEIYPYVTEGIGEDILPQNVDFDVIDLFEKVTDKDAAVYTRRLAREEGIFVGN
                     SAGAAIKGVLQLSGELKEDDVVVVLFHDHGSRYVGKIFNDDWMREQGFLEESIKTAAD
                     VIAKNATQKNKMLYSSELIAHAVANIRKFGYSQFPVVKEGKIVGSVDEDLIYEKLTQN
                     PENCNASLESIMKAPFPIVREETTLEEISALITPTNKAVLVDKI"
     gene            complement(1486..>1937)
                     /locus_tag="PKN22_10925"
     CDS             complement(1486..>1937)
                     /locus_tag="PKN22_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNS43261.1"
                     /translation="EGTVRSESYRLELEKFSPHIDLFQHACPQWVPIIENGLQDTEEG
                     KQLIKKDVEQLLAMSPQLDTILLACTHYPIIASYIETLVPPTVKVVAQGDIVATSLVD
                     YLQRHPEIENRLSKQRTVRYFTSGNPEEFDRQAREIIHLETKAQLML"
BASE COUNT          532 a          424 c          341 g          640 t
ORIGIN      
        1 cggagcgcca gaggcgcgaa ccactgccct gccggaggcg gggcgacgtc gagtttgtac
       61 cacaaacttg actatcctgc cacataaaac taaactatat tttatccacc aaaacagctt
      121 tgtttgttgg tgtaattaaa gcagatattt cttcaagagt tgtttcttct ctcacaattg
      181 gaaatggcgc tttcatgatg gattccaacg atgcgttgca attttctggg ttttgtgtca
      241 acttttcgta aattaagtct tcatcaaccg aacctacaat tttcccttct tttacaactg
      301 gaaattgaga atagccaaat ttgcgaatgt tggccactgc atgcgctatt aattctgaag
      361 aatacaacat tttatttttt tgcgttgcat tttttgcaat tacatcagcg gcagttttaa
      421 tgctttcttc caaaaatcct tgctcacgca tccaatcgtc gttgaaaatt tttccaacat
      481 aacgtgaacc gtgatcgtgg aacaatacca ccactacatc gtcttctttt aattcaccac
      541 taagctgcaa aactccttta atggctgctc cagcagagtt tcctacaaaa ataccttctt
      601 cgcgcgccaa tctacgagta taaacagcag catccttatc ggttactttt tcaaataagt
      661 caatcacatc aaaatcaaca ttttgcggaa gaatatcctc gccaattcct tctgttacat
      721 acggataaat ttcactttcg tcaaagattc cagtttcctt gtattttttg aacaccgaac
      781 cataggtatc aattccccaa attttaatgt ttggattttt ttctttcaaa tatttaccta
      841 ctccagaaat ggttcctcct gtaccaacac caacaacaaa atgggtgatt ttaccatctg
      901 tttgttccca aatttcaggc cctgttgatt cataatgcgc ctgacggttg gataaattat
      961 cgtattggtt tacgtaccac gaattaggtg tttctgcagc caatcttctt gatacagaat
     1021 aatatgaacg tgggtcggat gggtcaacag cggttggaca aaccaccacc tctgcaccta
     1081 ctgcacgaag gatatccatt ttttctttgg attgtttgtc gttcaacaca caaatcaact
     1141 tatatccttt gatgatacac gccaaagcca atcccatacc tgtgtttcca gatgttcctt
     1201 caatgatggt tcctccgggc ttgattaaac ctgctttttc agcatcttca atcattttca
     1261 ctgccatacg gtctttaaca gagttcccag gattagaggt ttctactttt gctaaaacgg
     1321 tcgcttttac gtcttttgta attttattta aacgaaccaa gggcgtattc ccaatcgttt
     1381 ctagaatgtt attgtaaatt ttcatacttt tatttagagt tacaaatgta atcaatggtt
     1441 ttgagaattg aaaggtgcat ttgtaatttt agcaacaaac tgtttttata gcatcaattg
     1501 tgcttttgtt tccaggtgaa tgatttcacg tgcttgacgg tcaaactctt ctggatttcc
     1561 agaggtaaaa tagcgtacgg ttctttgctt cgacaagcga ttctcaattt caggatgacg
     1621 ttgcaagtaa tccaccaaac tagttgcaac tatatcgcct tgtgctacaa cttttacggt
     1681 aggtggcacc aacgtttcaa tgtaggaagc aatgatagga taatgggtgc atgccaacaa
     1741 aatggtatcc aattggggcg acatggctaa taattgttca acgtcttttt ttataagttg
     1801 tttaccctct tcggtatctt gcaagccatt ttcgataatg ggaacccact gcggacaagc
     1861 gtgctgaaac aaatcgatat gaggcgaaaa tttttccaac tccaaacggt atgattctga
     1921 acgcaccgtt ccttctg
//
LOCUS       DAOQVQ010000465         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_76276_length_1936_cov_3.315789, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000465 DAOQVQ010000000
VERSION     DAOQVQ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76276_length_1936_cov_3.315789"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN22_10930"
     CDS             <1..516
                     /locus_tag="PKN22_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNS43262.1"
                     /translation="DNMRFPIVMKVTGPVHKTEVGGVILNIHDENQAMDAFNQLMKIP
                     DAKGVLVQEMLKGEELYCGAVKQGDFGHVVLCGLGGIFLELLNDTASALAPMSQLEVS
                     TMVESLKGYALIEGYRNKKGLDKNKYIDIIMRIGALVHLAPEIAELDLNPIMANETQM
                     TVVDVRIKIEK"
     gene            519..941
                     /locus_tag="PKN22_10935"
     CDS             519..941
                     /locus_tag="PKN22_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNS43263.1"
                     /translation="MKLRPIHVFATLCILFLAYSIDIYLKPMQVKEVTTINTHDMTEG
                     RLVWQKYNCQACHQLYHLGGYLGPDLTNVYSKYNGNCEVIKGFLKAGIRQMPSYNMTA
                     EEERLLIEFLKEVDATGSSDMKNFEVLPNGMIEQYGKR"
     gene            928..>1936
                     /locus_tag="PKN22_10940"
     CDS             928..>1936
                     /locus_tag="PKN22_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNS43264.1"
                     /translation="MERDKTATGRSFLSLAMVSLFVGLIGGVLASITYIKPDFMDNFQ
                     GLSKLRPIHVSFVMFWIIIGASGGVYMGMSILSKSKPIKWIANLQLSLWIVALLGIGY
                     SYLVGDLGGREYWEFNPIWALPILLAWLLQLINFIHLARTVKKWPVYVWMWMSGTVFF
                     LLIFIENYLWIFPYFRAHFITDMTIQWKVNGSLVGSFNQLIYATAFFMMDRINGTNEH
                     KIGSSKLAFAMYFLGLGNMMFNWSHHIYTLPTESYIRYVGYAVSMTEWIIFLRIVALW
                     KNSLTEFKKNYHFFPYRFIMASDIWVFINVGFACFMSIPAFNIYTHGTHFTVAHSMGT
                     TI"
BASE COUNT          579 a          338 c          409 g          610 t
ORIGIN      
        1 gacaacatgc gctttccaat tgtaatgaaa gttaccggac cagtgcacaa aacagaagtt
       61 gggggtgtta ttctcaatat tcacgatgaa aaccaagcaa tggacgcatt caaccaattg
      121 atgaaaattc cagatgcaaa aggcgtgttg gtgcaagaaa tgttgaaagg cgaagaattg
      181 tattgtggcg ctgtaaaaca aggagatttt ggtcacgtgg tgctttgtgg tttgggagga
      241 atatttcttg aattactcaa cgatacagct tcggcattgg cacctatgag tcagcttgaa
      301 gtatccacca tggttgaatc actcaaagga tatgccctca ttgaaggtta ccgcaacaaa
      361 aaaggactgg ataaaaacaa atacatcgac atcattatgc gcattggggc attggtacac
      421 ttggcaccag aaattgccga gttggattta aaccctatca tggccaatga aacacaaatg
      481 accgttgtgg atgtacgaat taaaatagaa aaataacaat gaaacttaga ccaatacacg
      541 tatttgctac tttgtgtatt ttgtttttgg cttactcaat tgatatttat ttaaaaccga
      601 tgcaggtgaa agaagtcaca acaatcaata cacacgacat gaccgaaggc agattggtat
      661 ggcaaaaata caattgccag gcatgtcacc aattatacca tttgggtggt tatctgggtc
      721 cagatttaac aaacgtttat tccaaataca acggaaattg cgaagttatt aaaggatttt
      781 taaaagctgg tattcgtcaa atgcctagtt acaacatgac agcagaagaa gaacgtttgc
      841 ttattgaatt tctgaaagag gtagatgcta ctggtagttc agatatgaaa aattttgagg
      901 ttttaccaaa cggaatgata gaacaatatg gaaagagata aaacagctac aggaagaagt
      961 tttttaagtc tggcaatggt ttctcttttt gttggactaa ttggtggtgt tttggcttcc
     1021 atcacctata tcaaacctga ttttatggat aattttcaag gattatctaa attacgtccc
     1081 atccacgttt cttttgtgat gttttggatc atcatcgggg caagtggcgg cgtgtatatg
     1141 ggcatgtcta tcctctccaa aagtaaacca atcaagtgga ttgccaacct ccaattgagt
     1201 ttgtggatag ttgccttgtt gggcattgga tattcttatc tagttggcga tttgggagga
     1261 cgtgaatatt gggagttcaa tcccatttgg gcacttccaa ttttgcttgc ttggcttttg
     1321 caattgatca attttattca cctggcaaga acggttaaaa aatggcccgt ttatgtttgg
     1381 atgtggatgt cgggaacggt gttttttcta ctcattttta tagaaaatta cttgtggata
     1441 tttccctact tccgtgcaca tttcataacc gatatgacca ttcaatggaa agtaaacggc
     1501 tcattggtag gttcttttaa ccaattaatt tatgctaccg cattttttat gatggaccgc
     1561 attaatggta ccaatgaaca taaaattgga agtagcaaat tggcttttgc catgtatttt
     1621 cttggattag gcaatatgat gtttaattgg agtcaccaca tttatacctt gcctaccgaa
     1681 agttacattc gctatgtggg atatgccgtg agcatgaccg aatggataat tttccttcgc
     1741 attgtggctt tgtggaaaaa ctcgcttaca gaatttaaga aaaactacca ttttttccct
     1801 tatcgtttta tcatggcatc cgatatttgg gtgtttatca acgttggatt tgcctgcttt
     1861 atgtccattc ctgcgtttaa catctacacg catggaacgc attttacagt tgcgcattcc
     1921 atgggaacaa ccatag
//
LOCUS       DAOQVQ010000466         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_76300_length_1936_cov_2.678894, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000466 DAOQVQ010000000
VERSION     DAOQVQ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76300_length_1936_cov_2.678894"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1936
                     /locus_tag="PKN22_10945"
     CDS             136..>1936
                     /locus_tag="PKN22_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43265.1"
                     /translation="MILQKIVQPQTGYYLEEQDYDGFLSVIIPEQRDNYVINPIMQDV
                     DNTNVPTGYTMIGTVGVTKVAQGPYNAYFWRLNLSAVGAFVSLSVTDVLSGYVTASIW
                     CKSSTASKFIIRVTFVDSEGAKVHLSNTKTTSDVWQQFTFTFKIVGLSSGPIAIRFDT
                     QDAGTIDFAAWQLEKGEYATTLIHGFAGEYYRWLGTPFTSASRRYVERIDGGRKVNLK
                     NLGMKLTAIDGLGIPDVDHATTALAFKYGSLFNGMVIKDRQYSMEFVLHSLSLEDLLC
                     ARNKVGKAIFNLNQPRCFVWQPLVCNVPVCDEVKFTAVYNDGFSLGLKSNFGEEIKLT
                     FTSYDIDMKKSIDETYSISTITDIVHATLIGVDKYGNAKLFPALPANTHKCYGMTISP
                     YDGNLYAIFDNTTSGRRGVLLQYNGVSWLKIAQVNSTTSSMTAVHAFGKYVYITCENS
                     QTITGQNGYAGTSDLAITRINLAIKTLESDTVMGKIKAGTTLAVDGVTALNPIVRKIV
                     SDKYGNIYVGGNFVGFENSASSTTTRHIAVFRTDNKWYNVDVDFSSYVGGVNTLYYDD
                     QRLRLYVGGNFYYPSFVTSSLSYNVFFYLNFETT"
BASE COUNT          660 a          261 c          362 g          653 t
ORIGIN      
        1 acaaataaat taaatgtgat ataataatat tatcattttt taatctccta attaaaaata
       61 tttataaaag acgttgcctt tacaggcgac gtttttttgt ttataataaa ataaagttat
      121 tttataagag gaaatatgat actacaaaaa attgttcagc cacaaacagg atactatctt
      181 gaagagcagg attatgatgg atttttatct gttataattc ctgagcaaag agataattat
      241 gttattaatc ctataatgca agatgttgat aataccaacg taccaacagg ttatacgatg
      301 ataggaactg ttggtgtgac taaggttgcc caaggaccat ataatgctta tttttggcgt
      361 ttaaatttgt cagcagttgg agcatttgtt tcattaagtg ttactgatgt attgagcggt
      421 tatgtaacag catctatttg gtgtaaatca tctacagcgt ctaaatttat tattagagta
      481 acatttgttg attctgaagg agcaaaagta catctttcaa atactaaaac aacaagtgat
      541 gtctggcaac aatttacatt tacctttaaa attgttggtt taagttctgg tcctatcgct
      601 attagatttg atactcaaga cgcaggaaca atcgattttg cagcatggca acttgaaaaa
      661 ggtgaatatg caacaacatt aattcatggt tttgctggtg aatattatcg atggcttgga
      721 acaccattca cttctgcatc aagacgatat gtagaaagaa tagatggtgg aagaaaagtt
      781 aatttaaaga atctaggaat gaagctaact gcaatcgatg ggttaggcat tccagatgtt
      841 gatcacgcaa caacagcact agcttttaaa tatggttcgc tatttaacgg catggtgata
      901 aaagaccgtc agtatagtat ggagttcgtg ttacatagtt tatctttaga agatttatta
      961 tgcgcaagaa ataaagttgg caaggcgatt ttcaatctaa atcaaccaag atgctttgtg
     1021 tggcaacctc ttgtctgtaa tgttccagtt tgtgatgaag ttaagttcac agccgtatat
     1081 aatgatggtt tttcacttgg attaaaaagt aactttggcg aagagataaa attaactttt
     1141 acaagttacg atattgatat gaaaaaatca attgatgaaa catatagcat cagtacgatt
     1201 acagatattg ttcatgctac attaattggt gtagataaat atggtaatgc gaaattattt
     1261 ccagccttac cagcaaatac tcataaatgc tatggtatga ctatctcacc ttatgatggt
     1321 aacttatatg ctatttttga taacacaact tctggaagaa gaggcgtatt gcttcaatac
     1381 aatggtgtaa gttggttgaa gatagctcaa gttaattcga caactagttc aatgacagca
     1441 gtacatgctt ttggcaaata tgtatatatt acttgtgaaa attcgcagac tattactggt
     1501 cagaacggat acgcagggac tagtgactta gccattacaa gaattaatct tgcgataaaa
     1561 acacttgaat cagatacagt tatgggtaaa ataaaagctg gcacaacttt agctgttgat
     1621 ggtgttacgg cattaaatcc aattgtaaga aaaattgtaa gtgataaata tggtaatatt
     1681 tatgttggtg gtaattttgt aggatttgaa aatagtgcat cttcaacaac aacaagacat
     1741 atagctgttt ttagaacgga taataaatgg tataatgttg atgtagattt ttcaagttat
     1801 gttggtggtg taaatactct atattatgat gatcaaagat tacgtttata cgtaggtggt
     1861 aatttctact atccatcatt tgtaacaagc tctttaagtt ataatgtctt cttttatctt
     1921 aattttgaaa cgactt
//
LOCUS       DAOQVQ010000467         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_76398_length_1934_cov_4.060671, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000467 DAOQVQ010000000
VERSION     DAOQVQ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76398_length_1934_cov_4.060671"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKN22_10950"
     CDS             complement(<1..717)
                     /locus_tag="PKN22_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS43266.1"
                     /translation="MEKAIKRLFSETAIYGLSTIIGRALNFLLVPLYVYVFSDPKDYG
                     VVSILYSWVAFLIVLLPLGMETAFYKFSHDDKYSPSTVYQNGLLTIILFNVGFWLVGS
                     ITSPQIADWMLLKGHSEYIILLLSIVCIDAIAALPLARLKNENKAKNFALIQLASIAV
                     NIISNVVLLLFFFDKSTPEQGVMYILISNLLASLVKPLLLYKDYLKLRFTFDSELAKQ
                     MLLYSIPLIFAGFAGIINETI"
     gene            complement(741..1517)
                     /locus_tag="PKN22_10955"
     CDS             complement(741..1517)
                     /locus_tag="PKN22_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNS43267.1"
                     /translation="MYNNILVEKNGQIVQITINRPSQLNALNKETIAELHTAIKTASE
                     DVSTGVIILTGSGDKAFVAGADIKEFAHFSIAEGGQLAQLGQQQLFDFIENLSIPVIA
                     AVNGFALGGGLELAMASHIRVASSNARMGLPEVSLGVIPGYGGTQRLAQLVGKGKANE
                     MVFTAGMIKADEALTWGLVNYVVEPEELLNKANEIATKILANSRTAITSAINAINAGF
                     KAGVNGYEAEIEEFGKCFGTADFKEGTTAFIEKRKANFRG"
     gene            complement(1529..>1934)
                     /locus_tag="PKN22_10960"
     CDS             complement(1529..>1934)
                     /locus_tag="PKN22_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNS43268.1"
                     /translation="QPYYEKVYSFDNNEWKQDQKAVFNVNIDDTTATYRFVLTLRTTT
                     DYDYSNIWLFWNSQTPTNEKVKEPFEMLITNPDGSWVGKSSGTIVENQLTFADRKIAV
                     PGNYIFTIEQAVTAEKVGEVLDLGLSVFKNKK"
BASE COUNT          578 a          416 c          352 g          588 t
ORIGIN      
        1 gatggtttcg ttgataatgc ccgcaaaacc tgcaaaaatt aacggaatag agtagagcaa
       61 catctgtttt gccaactcgc tgtcaaacgt aaaccgaagt tttaaataat ctttgtacaa
      121 caacaacggt ttcaccaatg aagccaacaa attggagatt aaaatataca ttactccttg
      181 ctctggcgta gatttgtcaa agaaaaagag gaggagcacc acgttgctaa taatgttgac
      241 agcaatggat gcaagttgaa tgagcgcaaa gtttttggcc ttgttttcgt ttttcaatcg
      301 tgccaacgga agagcagcaa tggcatcaat gcacacaata gacagcagta aaataatgta
      361 ttccgaatgg cctttgagca acatccaatc ggcaatttgc ggactcgtaa tcgaacccac
      421 caaccaaaaa ccaacgttga acaaaatgat ggttaacagt ccgttttgat aaactgttga
      481 aggagaatac ttatcgtcgt gcgaaaattt gtaaaatgca gtttccattc ccaaggggag
      541 caacacaatc aaaaaagcta cccacgaata gaggatggaa actacaccat aatctttagg
      601 atcagaaaaa acgtaaacat agagcggaac caacaaaaaa tttagtgcgc gaccaatgat
      661 tgttgacaaa ccataaatag ccgtttcgct aaaaagtcgt ttgatagctt tctccatgtt
      721 atcgtttcaa aaagcgcaat ttatcctcta aaattagctt ttcttttctc aataaatgcc
      781 gttgttcctt ctttgaagtc agccgttcca aagcatttac caaattcttc aatttctgct
      841 tcgtaaccgt ttactccagc tttaaaccca gcattaattg cgttgattgc cgaagtaatg
      901 gctgtgcgcg aattggctaa aattttagta gcaatttcgt ttgctttgtt gagcaattct
      961 tctggttcta caacgtagtt taccaatccc cacgtcaacg cttcgtcagc tttaatcatt
     1021 ccggctgtaa acaccatttc gttggctttt ccttttccaa ccaattgcgc caaacgttgc
     1081 gtgccgccat atccaggaat tacacccaaa gaaacttcag gcaatcccat gcgagcgtta
     1141 gacgaagcca cacgaatgtg cgaagccatt gccaattcca atccgccacc caatgcaaaa
     1201 ccgttaacgg cagcaataac agggatggaa agattttcaa taaaatcaaa caattgttgt
     1261 tgccccaatt gcgccaactg accgccttct gcaattgaaa aatgcgcaaa ttctttaatg
     1321 tcagctcctg ccacaaacgc tttgtcgccc gaaccagtaa gaataatcac acctgttgaa
     1381 acgtcctcgc tcgccgtttt gattgctgtg tgcaattcgg caattgtttc tttgtttaat
     1441 gcatttaatt gactaggacg attgattgta atttgaacaa tttgtccgtt tttttctact
     1501 aaaatattat tgtacatggc tttaaatttt attttttgtt tttaaacacg cttaatccta
     1561 aatccaatac ttcgcccact ttttcggctg ttactgcttg ttcgatggtg aatatgtagt
     1621 ttccaggaac tgcaatttta cgatcggcaa atgttaattg gttttctacg atagtcccag
     1681 aactttttcc tacccaacta ccgtcaggat tggtaattag catttcaaac ggttctttca
     1741 ctttttcgtt ggtgggcgtt tgcgaattcc aaaacaacca gatattactg tagtcataat
     1801 cagttgttgt tcgcaaggtg agcacaaaac gataagtagc tgttgtgtcg tcaatattta
     1861 cgttaaaaac tgctttttgg tcttgtttcc actcgttgtt gtcaaaactg tacacttttt
     1921 cgtaatacgg ttgt
//
LOCUS       DAOQVQ010000468         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_76467_length_1933_cov_4.055378, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000468 DAOQVQ010000000
VERSION     DAOQVQ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76467_length_1933_cov_4.055378"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PKN22_10965"
     CDS             complement(<1..1098)
                     /locus_tag="PKN22_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43269.1"
                     /translation="MKKLLLAFTTLLALQLNAQTKALNCTGNRYKEMIFTTVTKTADV
                     QYGTNYKQDGTTLENLKLDIYIPDGDTDTDRPLVLLAHGGSFIGGNKSDLQAQCEVLA
                     KMGYVAVSMSYRLLTLNAQVFLNKEMEFKKEVIRAIHDMRAAVRFFRKSVANGNPYGI
                     NEDLIVVGGYSAGAILADHVAYLDNISKVPADLVSYMNAQGGLEGNSGNAGYSSEPQL
                     VLSWCGAIMDTSWIEPGDQPYAGMHNVGDGVVPNLAAYPNPGFEIQVMLYGDSLMYKR
                     TLSQGVPSVYKSYPGNSHCNFPVGADDLFIDFVYDQICVQQLSLQKNPSTVLFSIYPN
                     PVETAFYIDVPSNVWDWKVSIVDMLGQTIHNE"
     gene            complement(1141..>1933)
                     /locus_tag="PKN22_10970"
     CDS             complement(1141..>1933)
                     /locus_tag="PKN22_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNS43270.1"
                     /translation="LTAVANDGSTFEDKRIVRVVSDIVPQLIGFDVVASFPHNISNFT
                     QGLEFSGSELFESTGQAGESKIAKVDIATGNDILKVALDATHFGEGITILRDTVYQLT
                     WTTGKCFLYDKNTLQILPKDFSYKGEGWGICNDGEYLITSDGSERLAFRNPKDFSIVK
                     TIEVYTHEQPVIRLNELEYANGFIFANIWMTSNVAIIEPQTGRVLGVLNCASLVSQGR
                     GNAGDVLNGIAYNKTDDTYYLTGKYWSKLFKIKLQQKIQELNTNQ"
BASE COUNT          595 a          404 c          290 g          644 t
ORIGIN      
        1 ttcattgtga attgtttgtc ctaacatatc aacgatagac actttccaat cccaaacatt
       61 tgagggtaca tcaatgtaaa atgctgtttc aactgggttt ggataaatag agaacaatac
      121 tgttgatgga tttttttgca acgacaattg ttgaacacaa atttggtcat acacaaagtc
      181 aatgaataaa tcatctgctc ctactgggaa attacagtgg ctgtttccag gatagctttt
      241 gtaaacacta ggcactcctt gagacaatgt tcttttgtac attaatgaat caccatacaa
      301 catcacttga atttcaaaac ctgggtttgg ataagctgct aagttaggca caacaccatc
      361 acctacattg tgcattcctg cataaggttg gtcacctggc tcaatccaag aagtatccat
      421 aattgcacca caccagctca acaccaattg aggctcacta ctataccctg catttccaga
      481 gtttccttcc aaaccacctt gtgcgttcat ataagaaact aaatctgctg gcaccttgct
      541 gatgttatcc aaataagcaa catggtctgc taaaattgct cccgcagaat aacctccaac
      601 cacaattaaa tcttcattga taccataagg atttccattt gcaactgatt ttctaaagaa
      661 acgaacagca gcacgcatgt catgaatggc acgaattact tcttttttaa attccatttc
      721 tttatttaag aaaacttgtg cattcaaagt caacaaacgg tagctcattg aaacagcaac
      781 atatcccatt ttagccaaaa cttcacattg tgcttgaagg tcagatttat taccccctat
      841 aaacgatcct ccgtgtgcta acaataccaa aggtctgtcg gtgtctgtat ctccatccgg
      901 aatgtagata tctaatttta aattttcaag tgttgtacca tcttgtttat agtttgtacc
      961 atattgaaca tctgctgttt ttgttacagt ggtaaaaatc atttctttgt aacgatttcc
     1021 tgtacaattc aacgcttttg tttgtgcatt taattgcaat gccaacaagg ttgtaaacgc
     1081 tagtaataat tttttcataa gctaaatttt aaattaatct tttcaaatat aagaaaaaag
     1141 ttactggttt gtatttaatt cttggatttt ttgttgcaat ttaattttaa acaattttga
     1201 ccaatatttc cctgtcaaat aataggtatc atctgtttta ttgtaagcaa ttccattcaa
     1261 tacgtctcca gcgtttcctc ttccttgcga aacgagtgaa gcacagttaa gcactcccaa
     1321 aactcttcct gtttgaggtt caataattgc cacgttgctt gtcatccaaa tgtttgcaaa
     1381 aataaacccg tttgcatatt ctaattcatt gaggcgaata actggttgct cgtgtgtata
     1441 aacttcaatg gttttcacaa tagaaaaatc ttttgggttt ctgaatgcta atcgctctga
     1501 accatctgat gtaatcaaat actcaccatc attgcaaatc ccccaaccct cgcctttgta
     1561 gctaaaatct ttaggcaaaa tttgcaaggt gtttttatcg tataaaaaac attttcctgt
     1621 tgtccatgtt aactgataaa cagtatctct taaaatcgtt attccttcac caaaatgagt
     1681 tgcatccaaa gcaactttga gtatatcatt gcccgttgct atgtccactt ttgcaatttt
     1741 actctcacct gcttgtccag tactttcaaa caattcactg ccgctaaatt ctaaaccttg
     1801 tgtgaaattt gaaatattat gcggaaagct tgctacaacg tcaaaaccaa ttaattgagg
     1861 tacaatatct gaaactacac gcacaattcg tttgtcttca aatgtagatc catcgttggc
     1921 aacagctgtc aaa
//
LOCUS       DAOQVQ010000469         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_77286_length_1920_cov_3.840751, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000469 DAOQVQ010000000
VERSION     DAOQVQ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77286_length_1920_cov_3.840751"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKN22_10975"
     CDS             complement(<1..613)
                     /locus_tag="PKN22_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNS43271.1"
                     /translation="MKFVHPGFLWALALLIVPIIIHLFSFRKYKTLYFSSLQFVQHIE
                     KQNKSTQKLKNLLILICRLLALALIILAFAQPYFPKNSSVQAGNSSLLCIHIDNSFSM
                     TMKGVEGELISEAKESAKQLINKAPLNTKILLSTNNLDGVESHITTKIEALDRIDKVK
                     PSPLVRSYDEVVKWQKDNISNHTQSSRFASVQFVYLSDFQKNTN"
     gene            complement(644..1237)
                     /locus_tag="PKN22_10980"
     CDS             complement(644..1237)
                     /locus_tag="PKN22_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNS43272.1"
                     /translation="MRFCLLLFFVLLLQWNFGQYQDSIQHLRDSFSLEILSEQSDVLN
                     SEEKNNIQSFDWFSINKDYRVKAKFKVKKGKKFEIPTSSGRTKLYQSRGYVVFELHQQ
                     KVKLYVYQDVKLSQQEAYKDYLLLPFKDLTNGKETYGAGRYLDLKMPKKSTVILDFNL
                     AYNPYCAYSHRYNCPIPPKENHLQISIQAGEKNPIEK"
     gene            complement(1245..>1920)
                     /locus_tag="PKN22_10985"
     CDS             complement(1245..>1920)
                     /locus_tag="PKN22_10985"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNS43273.1"
                     /translation="EEETAEMQRLFEAVDALYIADGHHRSASSVGLEDRRRRENRPVC
                     QNESSFLAFMIDEDLMQILEFNRFIKSLNGLTEQALLEQAAASFEIEKCQALQKPTQE
                     HEMIVAIKGNWYKFTCKPAIIDKNHPVNSLDAEILTRYLLQPILKIGDLKTDRNIEFI
                     GGTTPVEKIEHWLQKDKYEMAIFLHPVTIDQVKKVADNQLIMPPKSTWVEPKLRSGLT
                     IYDINE"
BASE COUNT          585 a          332 c          336 g          667 t
ORIGIN      
        1 gattggtatt tttttgaaaa tcagataaat atacaaattg aactgaagca aaacgactgc
       61 tttgtgtgtg attggaaatg ttgtcttttt gccatttcac cacttcgtcg tagcttctaa
      121 ccaagggcga gggtttaacc ttgtcaatac ggtccaatgc ctcaatttta gtggtaatgt
      181 gcgattctac accatccaaa ttattggtag aaagtaaaat cttggtgttc aaaggcgcct
      241 tattgataag ttgtttggca ctttcttttg cttcagaaat taattcacct tccacgcctt
      301 tcatagtcat agaaaatgaa ttgtcaatgt gtatgcacag caacgaagaa ttgcccgcct
      361 gtacagatga attcttggga aaatagggtt gagcaaatgc taaaataata agcgcaagcg
      421 ccaataaacg acaaataaga atgagtaagt tttttagttt ttgtgtgctt ttattttgtt
      481 tttcaatgtg ttgaacaaat tgtaagctcg aaaaataaag tgttttatat tttcgaaaac
      541 taaataaatg aataataatt ggcacaatta acagtgctaa tgcccatagg aaacctggat
      601 gaacaaactt catgattcaa agttaaagat attcggcaat aaattacttt tcaattggat
      661 ttttttcgcc cgcttgaata gaaatttgta aatgattctc ttttggcgga atggggcagt
      721 tgtaacgatg cgaataggca caatagggat tgtaggccaa attgaagtct aaaattacag
      781 ttgatttttt aggcattttc aaatccaagt aacgacctgc accataggtt tcttttccat
      841 tggttaaatc tttaaagggc aacaacaaat agtctttgta ggcttcttgt tgcgacaatt
      901 ttacgtcttg atatacgtat aattttactt tttgttggtg caattcaaaa actacatatc
      961 cccgcgattg gtacaatttt gttctgccag atgaagttgg aatttcaaat tttttacctt
     1021 tttttacttt aaatttagct ttaactcgat agtctttatt gattgaaaac cagtcaaatg
     1081 attggatgtt gtttttttct tcactgttca atacatcgct ttgttcactc aatatttcaa
     1141 gtgaaaaaga atcgcgtaaa tgttggatag aatcttggta ttgcccaaaa ttccattgca
     1201 acaacaaaac aaaaaataag agtaaacaaa aacgcattta ttgactattc gttgatatcg
     1261 taaatagtta aaccgctgcg caattttggt tctacccacg tagattttgg cggcatgatt
     1321 aattgattgt ctgccacttt tttcacttga tcaatggtaa caggatgtaa gaaaatagcc
     1381 atttcgtatt tatctttttg cagccaatgc tcaatttttt ctacaggtgt tgtgccgcca
     1441 ataaattcaa tatttctatc cgtttttaaa tcgccaattt tcaatatagg ttgcaacaaa
     1501 taacgtgtta aaatttcggc atccaacgag ttgacagggt gatttttatc aataattgcc
     1561 ggtttgcagg tgaatttata ccaatttcct ttgatcgcaa caatcatttc gtgctcttgt
     1621 gttggttttt gcaacgcttg gcatttttca atttcaaaag atgctgctgc ttgttccaac
     1681 aacgcttgtt cggttaagcc gtttaaactt ttaataaaac ggttgaattc aaggatttgc
     1741 atcaaatctt catcaatcat aaaagcaaga aaactgcttt cgttttgaca aactggtcta
     1801 ttttctcttc tacgtctatc ttccaaacca acagacgaag ccgagcggtg gtgaccatcg
     1861 gcaatataca acgcatcaac agcttcaaat aaacgttgca tttctgcagt ttcttcttcc
//
LOCUS       DAOQVQ010000470         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_77358_length_1919_cov_3.496245, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000470 DAOQVQ010000000
VERSION     DAOQVQ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77358_length_1919_cov_3.496245"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKN22_10990"
     CDS             complement(<1..288)
                     /locus_tag="PKN22_10990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNS43274.1"
                     /translation="MQLFYDAQITDSTQFHQLNETESRHICKVLRLKEGDTIGLQNGC
                     GLLATAVILEAHPKHCKVEIIERKIEVAPSFNVHIAICPTKMNERMEWLVEK"
     gene            complement(290..1294)
                     /gene="holA"
                     /locus_tag="PKN22_10995"
     CDS             complement(290..1294)
                     /gene="holA"
                     /locus_tag="PKN22_10995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNS43275.1"
                     /translation="MDHKLIIKNIKQKQFEKIYFLHGEEPYYIDLVCDAIEENAIDES
                     EKDFNQTIIYGKDVVIPQLISEVKSFPMMGEKRLVIVKEAQNIDKDDFELLAPLFENP
                     VDTTIFVICYKYKKYDSRTKLLKDAAKNGLVFVTEKLKDYKVVDWINKFVAESGYQIS
                     QKASMLLAENLGTDIGRIINELEKLQILVEKGTVINEVHVEENIGISKDYNFFELSNA
                     FADRDHLKAYKIVDYFDKNPKAGSIIPVISNLYAYHIKLMRLHFSPDKSQASLASILK
                     IAPFVVAQYTKAAKIYPPKKLAENIAILQEYDLMSKGVNNSSMSSGDLMKEMMFRLMN
                     "
     gene            complement(1296..>1919)
                     /locus_tag="PKN22_11000"
     CDS             complement(1296..>1919)
                     /locus_tag="PKN22_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS43276.1"
                     /translation="IEDETFSADSVKVTFKGVSAHPGYAKGIMVNSIKLASDFVAALP
                     KDRWSPETTEGRDGFVHPVSISGIMESTTVSFIIRDHVTEKLSKYEDELRGIAQMIVE
                     PHNGVSMEFVVKEQYRNMKEVLNSVPFVTEYAIEAMKRAGVEPKPMIIRGGTDGSRLS
                     FMGLPCPNLFTGEMGIHSKHEYVSVQDMEKAVRTMVELIKIWEEHVD"
BASE COUNT          556 a          383 c          324 g          656 t
ORIGIN      
        1 tttttcaacc agccattcca tgcgctcgtt cattttggtg ggacaaatgg caatatgaac
       61 gttgaacgat ggagcaactt caatttttcg ctcaataatt tcaactttgc aatgtttggg
      121 gtgtgcctct aaaattacag cagttgccaa caaaccacaa ccgttttgaa gtccaattgt
      181 atcgccttct tttaaacgga gaactttaca aatatggcgc gattcggttt cattcaattg
      241 atgaaattgt gtggaatcgg ttatttgagc atcgtaaaat agttgcatat caattcatta
      301 agcgaaacat catttctttc atcaaatcgc cactagacat ggatgaattg ttgactcctt
      361 ttgacattaa atcgtattct tgtaaaatag caatgttttc tgccaatttt ttgggtgggt
      421 atatttttgc tgctttggta tactgagcaa caacaaatgg tgctattttt aaaattgaag
      481 ccaacgaagc ttgcgattta tcgggtgaaa aatgtaaacg catcaattta atgtgataag
      541 catacaagtt acttattacc ggaataatgc ttcctgcttt agggtttttg tcaaaataat
      601 caacaatttt ataggctttc aggtgatcgc gatcggcaaa cgcattagaa agctcgaaaa
      661 agttgtaatc tttggagata ccaatgtttt cttccacatg tacctcgttg ataaccgttc
      721 ctttttctac taaaatttga agtttttcaa gctcattgat gattctacca atgtcggttc
      781 ccaagttttc ggccaacaac atagaagctt tttgcgaaat ttggtagccc gattcggcaa
      841 caaatttatt tatccaatca actactttat aatctttaag tttttcggta acaaagacca
      901 acccattttt ggcggcatct ttgagcaatt ttgtgcgcga gtcgtatttt ttatacttat
      961 aacaaataac aaaaatagtg gtatcaactg gattttcaaa taagggggca aggagctcaa
     1021 aatcatcctt atcaatgttt tgagcttctt taactatcac caatcgtttt tctcccatca
     1081 taggaaacga tttcacctct gatattagct gcggaataac aacatcctta ccataaatta
     1141 tcgtttggtt gaaatctttt tcgctttcat caatggcatt ttcctctatt gcatcacaaa
     1201 ctaaatcaat gtaatacggt tcttcaccat gaagaaagta aattttttcg aactgttttt
     1261 gcttaatatt ttttatgata agcttgtggt ccatcttaat ctacgtgttc ttcccagatt
     1321 ttaattaatt ccaccattgt tcgaactgct ttttccatat cttgaacgct cacgtattcg
     1381 tgttttgagt gaattcccat ttctccagta aacaagttcg gacaaggtaa tcccataaac
     1441 gataagcgag aaccgtcagt tccaccgcgg ataatcatcg gttttggttc aacaccagca
     1501 cgtttcattg cttcaatagc atattctgta acaaaaggaa cgctgttcaa tacttctttc
     1561 atgttgcggt attgttcttt taccacaaat tccattgaaa caccattgtg cggttcaaca
     1621 atcatttggg caattcctct taattcgtct tcgtatttag aaagcttttc ggtaacatgg
     1681 tcacgaatga tgaaactcac cgttgttgat tccataattc cactgataga aacagggtga
     1741 acaaaaccat ctcttccttc agtcgtttca ggcgaccaac gatcttttgg caaggcagcc
     1801 acaaaatcag aagccaattt gatggagtta accatgattc ctttcgcata accagggtgt
     1861 gcactcactc ctttaaatgt tacttttact gagtcagcag aaaatgtttc gtcttcaat
//
LOCUS       DAOQVQ010000471         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_77504_length_1917_cov_2.771751, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000471 DAOQVQ010000000
VERSION     DAOQVQ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77504_length_1917_cov_2.771751"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PKN22_11005"
     CDS             <1..1170
                     /locus_tag="PKN22_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS43277.1"
                     /translation="ALETVGIADEVRKIAIPMYGRTMHDLEGNLTFQPYGKENEAIFS
                     TSRAHLNCILMDFAEQKAQTKIHFNQQCIDFDSNKNCLTFKNTETGEITQHTSDLVFG
                     CDGAFSAVRFFGLQRTDRFGFQQNFIADGYREILLPANEDGTHKLAKNTLHIWPRGRF
                     MLIALPNEDGSYTCTLFMPHEGGENAFDKVNTKEEVTAFFQKTFPDFYALMPQIADEW
                     ENHPLSSLCIMKCSKYHYKKTVLMGDAAHATVPFYGQGMNAGFEDCTVMWQLMQKHNE
                     NWEAIFEEYSQLRKPDGDALQELSLFNYLVMRDFVADEQFLLRKKIEAKFSLLYPNKW
                     MPLYSQVTFSEIRYSDAHAAGERQSKIMDEIMLIPNIEAKWDSQEVMDKILSLVG"
     gene            1167..>1917
                     /locus_tag="PKN22_11010"
     CDS             1167..>1917
                     /locus_tag="PKN22_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43278.1"
                     /translation="MKKVLIVLLFLGFTQVGFSQRNLVPGKGKSKKDFFGGAADFKEY
                     RPFGLQLSVGPTFTIPTRPRPITQDFISSGRSYQLTTKANGIPGIFAEIGMIHLPQKR
                     SKLSQAWNYIFISYIDWGIGFKMLNGAETTRISQLDPVTGNVLNTAEQTGTFNNLFVT
                     ARVAVHKNFYIGKKYFIDNGFGVNVDFNFKRNTTNYTPFVENAVPGKHYFHEPVMAHI
                     HYELGFGFRLSRRSMLIPSVQVPIFGIYEWRK"
BASE COUNT          612 a          335 c          390 g          580 t
ORIGIN      
        1 gcccttgaaa cggttggtat tgcagatgaa gtgagaaaaa ttgccattcc tatgtatggt
       61 cgcaccatgc acgatttgga aggtaacctt acttttcaac cgtatgggaa agaaaacgaa
      121 gctatttttt ctacttctcg cgcacatttg aattgcattt tgatggattt tgctgaacaa
      181 aaagcacaaa caaaaattca tttcaatcaa caatgcattg attttgatag taataaaaat
      241 tgtttaactt tcaaaaacac agaaacagga gaaattactc agcacacatc agatttagtg
      301 tttggttgcg atggtgcttt ttcagccgta cgtttttttg gtttgcaacg caccgatcgt
      361 ttcggatttc aacaaaattt tattgcagat ggttaccgcg aaattttatt gccagctaat
      421 gaagatggta cacataaatt agcaaaaaac actctgcata tctggcctcg aggtcgtttt
      481 atgttgattg ctcttcctaa cgaagatggt tcatacacat gtacgctttt catgccgcac
      541 gaaggtggtg aaaatgcttt tgacaaagtg aatacgaagg aagaagttac ggcatttttt
      601 caaaaaacgt tcccagattt ttatgcattg atgcctcaaa ttgcagatga gtgggaaaac
      661 catccactat catcgttgtg cattatgaaa tgtagcaaat accattataa aaaaaccgta
      721 ttgatgggtg atgcagcaca tgcaacagtg ccgttttatg gtcaagggat gaatgctgga
      781 tttgaagatt gcacggtaat gtggcaattg atgcaaaaac acaacgaaaa ttgggaggct
      841 atttttgaag aatacagtca attaagaaaa cccgatggcg atgcactgca agaattgtcg
      901 cttttcaatt accttgtaat gcgtgatttt gtagctgacg aacagttttt attgcgcaaa
      961 aaaattgaag caaaattcag tttattgtac ccaaataagt ggatgccgtt atattctcaa
     1021 gttacttttt cagaaattcg ctattcggat gcacacgcag cgggcgaacg tcaaagtaaa
     1081 attatggatg aaattatgct aattccaaac attgaagcga agtgggattc gcaagaagtt
     1141 atggataaaa ttttaagttt agtaggatga aaaaagtttt aattgttttg ctctttttgg
     1201 gatttacgca agtaggattt agtcagcgca accttgtgcc aggaaaagga aaaagtaaaa
     1261 aagatttttt tggtggagca gccgacttta aggaataccg cccgtttggc ttgcaattat
     1321 cggttggacc aacatttaca attcctacgc gtccacgtcc cattacacaa gattttattt
     1381 cttcaggaag atcgtatcaa ttgactacca aagccaatgg aattccagga atttttgctg
     1441 aaattggcat gatacattta cctcaaaaac gttctaagtt atcgcaagcg tggaactaca
     1501 tttttatttc ctacattgat tggggaattg gttttaaaat gctcaacggt gcagaaacaa
     1561 cacgcattag tcagcttgac cctgtgactg gaaatgtgct caatacagcg gagcaaactg
     1621 gaacttttaa caaccttttt gttacagcac gtgtggcagt gcacaagaat ttttacatcg
     1681 gtaaaaaata ttttattgac aatggatttg gagtaaacgt tgattttaac ttcaaacgca
     1741 acactaccaa ttacacgcca tttgtagaaa acgctgtgcc gggcaaacat tattttcacg
     1801 aacctgtaat ggcacatatc cactatgaat taggctttgg atttcgatta agcagaagaa
     1861 gtatgctcat tccatcggtg caagttccaa tttttggaat ttatgaatgg cgcaaag
//
LOCUS       DAOQVQ010000472         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_78199_length_1907_cov_4.219762, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000472 DAOQVQ010000000
VERSION     DAOQVQ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78199_length_1907_cov_4.219762"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKN22_11015"
     CDS             complement(<1..560)
                     /locus_tag="PKN22_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43279.1"
                     /translation="MYNIDFYSLLYSPINIISTLIFLLIIIGMMLFKSNKISLTDPKK
                     AKLFRLNVYHKLLLSSVFAVYYIVIMQGGDTYAYWKSTEAFQNLMFHNFDHFWELMTT
                     PPTMEKFHGYFNYKIGYPMRFIYVEEESFFVVKILYIFRLLTLGSYFATTFIFAYITA
                     SASWKIYELVSNMGIFKKKLLQFFIL"
     gene            complement(646..1008)
                     /locus_tag="PKN22_11020"
     CDS             complement(646..1008)
                     /locus_tag="PKN22_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNS43280.1"
                     /translation="MNWIKLSLISVVVGIVYLSLMPPDGPVKVTVNDKIGHFIAYSVL
                     TINAGLLVSSKKWIGVGILAFLLSILLEFLQHYVPGRHTDFRDLIANGTGVVIGLVVL
                     VTMSKQLLHLLRKIKIVS"
     gene            complement(995..1813)
                     /locus_tag="PKN22_11025"
     CDS             complement(995..1813)
                     /locus_tag="PKN22_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNS43281.1"
                     /translation="MQGLVYRPENFDSTKRYPLLVYFYEMYSDDLHNHYVPKPTASII
                     FPTEYASAGYVVFIPDIRYEPGHPAKSAYDCIMSGTDAVLKKFPQIDASRMGLQGQSW
                     GGYQTAQLITMTNRYKAAMAGAPVSNMFSAYGGIRWGSGMSRQFQYEKTQSRIGKTIW
                     EAPELYVENSPVFHLPKVTTPLLIMHNDKDGAVPWYQGIELFAGLRRLGKTAWLLNYN
                     DDDHNLMKIPNRRDLSRRMRQFFDHYLQNQPAPRWMVEGIPAVKKEKETRYELD"
BASE COUNT          626 a          375 c          318 g          588 t
ORIGIN      
        1 aaaagaatga aaaattgaag tagttttttc ttaaatatcc ccatgttgga tactaattca
       61 tagattttcc atgaagcact tgcagttata taagcaaaaa tgaaagtggt tgcaaaataa
      121 ctccctaatg ttagtaatcg aaagatatac agaattttaa ccacaaaaaa agattcttcc
      181 tccacataga taaaacgcat aggatagcca attttgtaat taaaataccc atgaaatttt
      241 tccatcgttg gaggtgttgt catcaactcc caaaaatgat caaaattgtg aaacatgagg
      301 ttttgaaatg cttcggttga tttccaataa gcataagtat ccccaccttg cattatcaca
      361 atataataaa ccgcaaaaac agacgaaagc aacaatttgt ggtaaacatt taaccgaaac
      421 agtttggcct ttttagggtc tgttaacgaa attttattcg atttaaaaag catcatccca
      481 ataataatga gtagaaatat caacgtagag atgatattta tggggctata cagtaacgaa
      541 taaaaatcta tgttatacat gcttttacgt tagtctcatt gttgaatgga aatgcaagat
      601 agaaattttt caacgattgc ctattttttc gctccaaatc tttgattagg acacaatttt
      661 aattttacgc aaaagatgca atagctgctt gctcattgta actaaaacca ctagtccaat
      721 tacaactcca gttccgttgg caatcaaatc ccgaaaatcg gtgtggcgac caggaacata
      781 atgttgtaaa aattccaaca agatacttag taaaaatgcc aaaataccaa ctccaatcca
      841 ttttttagaa ctgaccaata aaccagcgtt tatcgttaaa acactgtaag caatgaaatg
      901 cccaatttta tcattaaccg taactttcac cggtccatcg ggtggcatta aactcaaata
      961 aacaatgcca actaccacag aaatcaagct taatttaatc caattcataa cgtgtttctt
     1021 tctctttttt aactgccgga attccctcta ccatccatct tggcgcaggt tgattttgta
     1081 agtaatggtc gaaaaactga cgcatgcgtc tgcttaaatc tcttctgttg ggaattttca
     1141 tcaaattgtg atcatcatcg ttgtagttca acaaccaagc ggtttttcct aatcgtctga
     1201 gtccagcaaa caactcaata ccttgatacc acggaacagc accatcttta tcgttgtgca
     1261 ttatcaacag tggtgttgta acctttggca aatgaaaaac aggtgaattt tctacataca
     1321 attcgggggc ttcccaaatg gtttttccaa ttctactttg tgttttttcg tattggaatt
     1381 ggcgactcat cccagaaccc cagcggattc ctccataagc cgaaaacatg tttgaaactg
     1441 gtgctccagc catggcagcc ttgtaacgat tggtcattgt aatcaattgt gcagtttggt
     1501 aaccacccca actttgacct tgcaagccca tacgtgaagc atctatttgt ggaaactttt
     1561 ttaacacagc atctgttccg ctcataatac agtcataagc acttttggct gggtgaccag
     1621 gttcatagcg aatgtctgga ataaatacaa catatcctgc cgatgcatat tcggttggaa
     1681 aaatgatgga tgctgttggt tttggaacat aatgattgtg taaatcgtct gaatacattt
     1741 cgtagaaata aaccaataac ggataccttt tggtggaatc aaaattttct ggacgataga
     1801 ccaatccctg caaatcaatt cctttgtatg atttccacga aataagttca accgttgccc
     1861 aattgtaatt gacttgttgt ggattagtga cagaaattta cttccaa
//
LOCUS       DAOQVQ010000473         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_78817_length_1898_cov_3.500271, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000473 DAOQVQ010000000
VERSION     DAOQVQ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78817_length_1898_cov_3.500271"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKN22_11030"
     CDS             complement(<1..808)
                     /locus_tag="PKN22_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNS43282.1"
                     /translation="MATKEIAAIVAPREPHFVGDGFRVHNFIPSMPGMDMKSMSPFIM
                     LDYNSKYYFEPSVRPKGVGTHPHKGFETVTIAYKGKVQHRDSSGGGGIIGEGDVQWMT
                     AGSGILHQEFHEVEFSKKGGDFQMVQLWVNLPAKDKKTAPKYQAIQNSEMPIYDFGDG
                     SKIEVIAGDYKNLKGKAFTFTPVHLMNAKLIKGKKADFSFPADYTTFLLVLEGAITVN
                     QTEFVATDHLLLFDRKGEDFSIEANENAVVLVLSGEPINEPIAAHGPFVMN"
     gene            1000..>1898
                     /locus_tag="PKN22_11035"
     CDS             1000..>1898
                     /locus_tag="PKN22_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43283.1"
                     /translation="MKKNLLLVTALIASQVSFGQLTQTNEPAIGASLPMYKVTDTATV
                     SSLGMLGVTGNGVTWDYSSVVMETAASGVVGVTDASTDTHAAEFPTSTKVISQGTIQQ
                     FFNSTSSARTSQGFVFNEPTFGEIVVVLDSDEAIQLTYPFAYGNSNADNYAGSTELNI
                     IGPVTAAVTGDVYSSIDGTGTLKLADNQIANVFRLTTIDTTLVDVPSMMQVEVIRRQM
                     EYYDLSDVSSQNLPIFVDAYISISGMGAQRDVLSKYYATAGLENNTINNVVVFPNPSA
                     GEFAISGEFVKANVEVTDVAGKVV"
BASE COUNT          579 a          333 c          333 g          653 t
ORIGIN      
        1 tgttcataac aaaaggtcca tgtgctgcaa ttggctcgtt gataggttcg ccacttagta
       61 ccaaaacaac agcattttca ttggcttcta ttgaaaaatc ttctcctttt ctatcaaata
      121 aaagcaagtg atctgttgct acaaattccg tttgattaac agtaatagct ccctcaagca
      181 ccaataaaaa tgtggtgtaa tcggcaggga agctaaaatc agctttttta cctttgataa
      241 gtttggcgtt cataaggtga actggagtaa acgtaaaagc ttttcctttc aagtttttgt
      301 aatcgccagc aatcacctca attttgctac catcaccaaa atcataaata ggcatttcgc
      361 tgttttgaat tgcttggtat ttgggtgctg tttttttatc ttttgctggt aaatttaccc
      421 acaattgaac catttgaaaa tcaccaccct ttttgctaaa ttctacttcg tgaaattctt
      481 ggtgtaaaat tccagaacct gcagtcatcc attgcacatc accttcacca attattccac
      541 caccaccact gctgtctcta tgttgcactt tgcctttgta agcaatagtt acagtttcaa
      601 aacctttgtg ggggtgtgtg ccaactcctt ttggtcgaac gcttggttca aagtaatatt
      661 ttgaattgta atctagcata ataaaaggcg acatgctttt catatccatt ccaggcatac
      721 ttggaatgaa attgtgcact ctaaaaccat ctccaacaaa gtggggctct cttggagcta
      781 cgattgctgc tatttctttt gttgccatat cattttttta tacaaaatta gtgtagaacc
      841 aaatcttttg cattgatgta agttaagaat tgaacgtttt ttttgtttta aaacaactaa
      901 ttacttgtta tcttcgtctt ctaattgcat tattttatga gcaaataatt ttagaataat
      961 gattaattac taaattcgaa taacttaaaa acaagattta tgaaaaaaaa tttattatta
     1021 gttacggcat taattgcatc acaagtttct tttgggcaat taacacaaac aaatgaacca
     1081 gcaattggag cttcacttcc aatgtataaa gtgacagata ctgcgacagt ttcttcttta
     1141 ggaatgttgg gtgttacagg aaatggtgta acatgggatt atagctcagt ggttatggaa
     1201 actgctgctt caggagtagt gggtgtaacg gatgcatcaa cagatacaca tgctgctgaa
     1261 tttccaactt caacaaaagt tatttcacaa ggaacaattc aacaattctt taactctact
     1321 tcttcagcaa gaacatcaca aggttttgtt ttcaatgaac ccacttttgg agaaattgtt
     1381 gttgttttag attcagatga agcaattcaa ttaacttatc catttgcgta tggaaattca
     1441 aacgctgata attacgcagg ttcaactgaa ttaaatatta ttggaccagt tactgctgct
     1501 gttactggtg atgtgtattc atcaatagac ggaacaggta ctttgaaatt ggcagataat
     1561 caaattgcaa atgtatttcg tttaactaca attgatacaa ctttggtaga tgttcctagt
     1621 atgatgcaag ttgaagtgat acgtcgtcaa atggaatatt acgatttatc agacgtttct
     1681 tctcaaaact taccaatttt tgtggatgct tacatttcta tttctggaat gggtgcacaa
     1741 cgtgatgtgt tgtctaaata ctatgcaact gcaggtttag aaaacaatac aatcaataat
     1801 gtggtagttt tccctaaccc atcagctggt gaatttgcta tttctggtga gtttgtaaaa
     1861 gcgaatgttg aagtaactga tgttgctgga aaagtagt
//
LOCUS       DAOQVQ010000474         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_78933_length_1896_cov_3.834329, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000474 DAOQVQ010000000
VERSION     DAOQVQ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78933_length_1896_cov_3.834329"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKN22_11040"
     CDS             <1..531
                     /locus_tag="PKN22_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43284.1"
                     /translation="FDFIKGWSAVAYPSYVSVDRYIKYGLSAELTLSFNQYSGGKTVN
                     ATKNAGFMFMTDLALKYSIYEYLYKKASWFDPYLGVGMSFTYNSATSPNAYPTFNVPF
                     GTNFIFRKGWGIRLQANAKFGLVPNFYRTNGNYMQYTAGVIYRFQKSEINKSNDKPRY
                     PWTRKKPGKYKGGKSK"
     gene            562..879
                     /locus_tag="PKN22_11045"
     CDS             562..879
                     /locus_tag="PKN22_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43285.1"
                     /translation="MINLDLIKLYAMETNKSTPQLVEKEQIPFLPFKNIIIADKNIEL
                     EKQLEQAVTLGNAHHTKVSIVFTDDDGLKRVETTIWAKGEKFICLKGGIWLPIARIIA
                     VNF"
     gene            1008..>1896
                     /locus_tag="PKN22_11050"
     CDS             1008..>1896
                     /locus_tag="PKN22_11050"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNS43286.1"
                     /translation="MKNFNIFLKIFLVVTIFTISIGKSFGQMEFPEDMVKVKFSVEQT
                     NETAYIVADVSVVPKWHINASVLPKGSFGLPTKFILNKSKDFQLVGGLIEPKPIEKYD
                     ELADENLAYHEGKFKIKQKIKVLSSKDFELSGSFNYQTCNEVKCLPDYTYEFKVKVKG
                     VEGAADESSVDETIVPTNEDAVQSDVSNEQTTVETPTVGKQDKMQASEAPLSKKHSIW
                     VIFGLSFLSGLLALITPCVFPMIPMTVSYFTKTSKTKAKGIKNATTYGFFIVAIYVAL
                     GALVMLTGSASKLNEMSTNP"
BASE COUNT          677 a          266 c          339 g          614 t
ORIGIN      
        1 tttgatttta taaaaggatg gagtgctgtg gcttatcctt catacgtttc tgttgatcgt
       61 tatatcaaat atggcttaag tgcagaattg actttatcat ttaatcaata ttctggtgga
      121 aaaactgtga atgcaacaaa aaatgctggt tttatgttta tgacagatct ggcattaaaa
      181 tatagcattt atgaatattt gtacaaaaaa gcaagttggt ttgatcctta tttaggtgta
      241 gggatgagtt ttacatacaa ttcagcaaca tcacccaatg cgtatccaac tttcaatgtg
      301 ccatttggaa ctaattttat ttttagaaaa gggtggggaa ttcgattaca ggcaaatgca
      361 aaatttggat tagtaccgaa cttttatcgt actaacggaa attacatgca atacactgca
      421 ggcgtaattt atcgtttcca aaaatcagag attaacaaaa gtaacgataa gccacgttat
      481 ccgtggactc gtaagaaacc tgggaaatac aaaggaggaa aatcaaaata aaattgtgtt
      541 ttagttgcat atattcgtaa aatgattaac ttagatttaa tcaaattata tgctatggaa
      601 actaataaat caactcctca actagtagaa aaggaacaaa ttccgttttt acccttcaaa
      661 aatattataa ttgctgataa aaatattgaa ttagaaaagc aattagaaca agctgtaacg
      721 cttgggaatg cacatcatac taaagtttct attgttttta ctgatgacga tggattaaaa
      781 cgagtggaaa ctacaatctg ggcaaaaggt gaaaagttta tttgtctaaa aggtggaatt
      841 tggttgccaa ttgcacgtat aatcgcggtg aatttttagt ttgggttttc aagaatgact
      901 ctaatttatt ttaagtttaa tccttaaata attgtaaaaa taaaagcaac tatagattta
      961 gttgttaatt ttacaactaa tcatttataa aactagcaat tgatagcatg aaaaacttca
     1021 atatcttttt aaaaatattt ttagttgtta ccatttttac aatctctatt ggaaaatctt
     1081 ttggtcagat ggaatttcct gaagacatgg taaaggttaa attttctgtt gaacaaacaa
     1141 acgaaacagc atatattgtt gcagatgttt ccgtagttcc aaaatggcac atcaacgctt
     1201 cggttttacc gaaaggaagt tttggtttac ctaccaaatt tattttgaat aaatccaaag
     1261 attttcaatt ggttggtgga ttgattgaac caaaaccgat tgaaaaatac gatgaattag
     1321 cagatgaaaa tttagcatat cacgagggga aatttaaaat taaacaaaaa attaaagttc
     1381 tttcatctaa agattttgaa ttgtcgggtt cttttaatta ccaaacatgt aatgaagtaa
     1441 aatgccttcc agattatacc tatgaattta aggtaaaagt taaaggcgtt gaaggtgcgg
     1501 cagatgaatc aagtgttgat gaaacgatag tgccaacaaa tgaagatgct gttcaatcgg
     1561 atgtatcaaa tgagcaaaca acagttgaaa caccaacagt tggaaagcaa gataaaatgc
     1621 aagcttcaga agcaccgctt tctaaaaaac attccatttg ggttatcttt ggattgtctt
     1681 ttttaagtgg tttgttggca ttgattacac cgtgcgtatt tccaatgata cctatgacgg
     1741 tgagctattt tacaaaaaca tctaaaacaa aagcaaaagg gataaaaaat gcaacaactt
     1801 atggtttttt tatcgttgca atttacgtgg ctttaggtgc tttggtgatg ctgacaggtt
     1861 ctgcaagtaa actcaatgaa atgtcaacca accctt
//
LOCUS       DAOQVQ010000475         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_79338_length_1890_cov_4.034877, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000475 DAOQVQ010000000
VERSION     DAOQVQ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79338_length_1890_cov_4.034877"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(395..841)
                     /locus_tag="PKN22_11055"
     CDS             complement(395..841)
                     /locus_tag="PKN22_11055"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNS43287.1"
                     /translation="MSAIRITKSFTFDMAHALEGYNGLCKNIHGHTYVLHVTVLGKPI
                     DENSNPENGLVMDFGDLKKIVKKGVLDTFDHALVIKEGSSILKSLNLTENERLITTPF
                     QPSCENLLIHYVGIIQSLLPQQVKLVAVRLNETPTSYAEWLLEDQL"
     gene            complement(911..1357)
                     /locus_tag="PKN22_11060"
     CDS             complement(911..1357)
                     /locus_tag="PKN22_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNS43288.1"
                     /translation="MFTKACEYGIRACLIVAQYSLNETRISLKEIAKLVDSPEAFTAK
                     ILQKLVKSKILYSIQGPQGGFGMTPENIKNTTLERIVYAIDGDSSYTACVLGLKDCSD
                     TNPCPAHKKYKFIKAEFLEMIHKTTVDEMVNDIENGLAFLKNNTGC"
     gene            complement(1361..>1890)
                     /locus_tag="PKN22_11065"
     CDS             complement(1361..>1890)
                     /locus_tag="PKN22_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNS43289.1"
                     /translation="FRPLEESIRDVNKAISDARAKGYLLGMKLVRGAYVEKEREYAKR
                     DGVPSPVFDTKEGTDRSYDTALEICLRENSLINTCLATHNQASVELAVQLIEEFKIEN
                     HHQKIFFSQLYGMSDNLTFNLAASNYNSSKYVPYGEVEKAIPYLMRRAEENSSIEGQV
                     GREYELLLLEKNRRK"
BASE COUNT          559 a          371 c          304 g          656 t
ORIGIN      
        1 aatttcttcc gaccattcgt gctcaattga gcgcctttct tgttatagga atttgcaaaa
       61 tagctattgt ttccctcctt tgaggaggga ttaagggagg tgtgacccct agaatcataa
      121 aataatcaac taaatcccat ttaaacctta ccttactacc gttataaatc cttcttttgt
      181 taaaaggcaa ttttgcaatt ggcagttaac aatcgtatcg tccaatgtaa atatcacctc
      241 ataaaaatac acaccatcta ttgcgggtgt gctccacagt gggttgaagc ctggtgttaa
      301 gttgccttga gaacaattat ttctctcaca aaagacacaa ataatagtta ttcttctctc
      361 accaacagtg gcaccaaaag cctaactatt tcaactacag ttggtcttca agcaaccatt
      421 ctgcataaga agttggagtt tcgtttaagc gaacagctac caattttact tgttgtggaa
      481 gtagggattg tatgatacct acgtaatgaa ttaataagtt ttcgcaactt ggttgaaaag
      541 gagtggtaat taaacgctcg ttttctgtta aattcaatga ttttaaaatg gacgaacctt
      601 ctttgataac caatgcatga tcaaatgtat ctaaaactcc ttttttgacg atttttttta
      661 aatcaccaaa atccatcact aaaccatttt ctggattgga attttcatct attggtttac
      721 ctaaaactgt aacgtgtaac acgtatgtat gaccgtggat gtttttacaa agaccgttgt
      781 atccttcaag ggcgtgcgcc atgtcgaatg taaaactctt tgtgatacga attgcagaca
      841 ttttaatttt tttgcaaaga tacgcttaaa aagcaataga aagctatttt tttggaaatg
      901 taaagaacaa ttaacagcct gtattgtttt ttaagaatgc caatccgttt tctatatcgt
      961 ttaccatttc gtcaaccgtt gttttgtgaa tcatttccaa aaattctgcc ttgataaact
     1021 tgtatttttt gtgcgccgga caagggttgg tgtctgaaca atcttttaaa cctaaaacac
     1081 atgccgtgta ggatgaatcg ccatcaatgg cataaacaat gcgctctaat gttgtgtttt
     1141 ttatgttttc gggtgtcatt ccaaaacctc cttgtggtcc ttggatggag tataaaattt
     1201 tactttttac caatttttgt aaaatcttgg ctgtaaacgc ttcgggcgaa tctaccaatt
     1261 tggcaatttc ctttaaactt attcttgttt catttaatga atattgagcc acaattaaac
     1321 aagctctaat accatactca cacgcttttg taaacatttt ttactttctc ctattttttt
     1381 caagtaataa taattcatac tctctaccaa cctgaccctc aatggatgaa ttttcttcgg
     1441 ctcttcgcat caaatatgga atagcttttt ctacctcacc ataaggcacg tatttggaag
     1501 aattgtaatt ggacgcagct aaattaaaag ttagattgtc actcattccg taaagttgcg
     1561 aaaaaaatat cttttggtga tgattttcta tcttaaattc ttcaattaat tgcacggcaa
     1621 gttccacact tgcttgatta tgcgtggcta aacacgtgtt tataagacta ttttctctta
     1681 aacaaatttc caatgctgta tcatacgaac ggtcggttcc ttctttggtg tcaaaaacag
     1741 gactcggtac accatctctt ttggcatatt cgcgttcttt ttcaacataa gcaccacgca
     1801 ccaatttcat acccaataaa tatccttttg cacgtgcatc agaaatagct ttgttcacat
     1861 cgcggatcga ttcctccaac ggtctaaaat
//
LOCUS       DAOQVQ010000476         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_79683_length_1885_cov_2.884699, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000476 DAOQVQ010000000
VERSION     DAOQVQ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79683_length_1885_cov_2.884699"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKN22_11070"
     CDS             <1..891
                     /locus_tag="PKN22_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43290.1"
                     /translation="PDYNNTIFIIFGDHGNHDLLFSGLDRYKNSMLIYSPLIKQPKTI
                     RSVSTHLDIAPSILNFLRINYPAMQLPSRVPFYGSELDLNPNYECKRTMPLNTIQHEN
                     SHLVMGDYLMMDKTLYKLTKNLTPEIYRNENKQKQLEKQLNLYNQLVANYFKCKFFKL
                     YENRVAVDNDILKSEYIYIGDYTTVEAHWKKVRLECDLDVFLPDETHLDSIPKLTCSI
                     EKMYSGKNNEVVIWKNTAPKNNSKFKKNSWNRVTYIIDINVSNLSKMNKKNQLAFYLY
                     NEDKKLHKMKNIVFKLYVLK"
     gene            1025..>1885
                     /locus_tag="PKN22_11075"
     CDS             1025..>1885
                     /locus_tag="PKN22_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43291.1"
                     /translation="MKAKELIHKIIPATLALYASLLVVHVYQIIMQPSQYLGQPVLFK
                     YHLKGFVMDLEFTFIFGTVVLALCLLFYKLLGKYTVVLIHLLNVIALFANISLILYFL
                     EANQPLGKVFFELSSTELKMASNPESFLNIVVLLALVGIITFYILTYYLFRKYSEHKW
                     LKIGVVTLWLFSLVAIPWSILESKDKVGDMVANNKGLYFLNEARQYFFNTDASNFPFT
                     EKDLRLMDASFFNNRHPEDIKHPFLHDFTNESSLNELLHHDSKRPPNIVYIIVESLSN
                     HMFGEGADVSG"
BASE COUNT          660 a          303 c          286 g          636 t
ORIGIN      
        1 cccgattata acaacaccat tttcatcatt tttggtgatc acggtaatca cgatttactg
       61 ttttctggtt tagaccgtta caagaacagt atgttgattt attcgccatt gataaaacaa
      121 cccaaaacca tccgctctgt ttccactcat ctggatattg caccaagcat attgaatttt
      181 ttacgtatca attacccagc gatgcaattg ccttcaagag ttccttttta tggaagtgaa
      241 ttggatttaa accccaacta cgaatgtaaa agaaccatgc cattaaacac cattcagcac
      301 gagaattcgc atttggtaat gggcgattat ttaatgatgg acaaaacact ttataagcta
      361 acaaaaaatt taacgcccga aatttatcga aatgaaaaca agcaaaaaca gttagaaaaa
      421 cagttgaact tatacaatca acttgtagca aactatttca aatgtaaatt ctttaaatta
      481 tatgaaaatc gtgttgctgt tgataatgat attcttaaat ctgaatatat ttacattggt
      541 gactatacta cagtagaagc tcattggaag aaggttcggc ttgaatgtga tttagatgtt
      601 ttcttgcctg atgaaacaca tttggactct attccaaaat taacttgctc tattgaaaaa
      661 atgtatagtg gtaaaaataa tgaagtagtt atttggaaaa acactgctcc caaaaacaat
      721 tcaaaattca aaaaaaatag ttggaataga gtgacatata tcatagatat taatgtgagt
      781 aatttatcaa aaatgaataa aaaaaatcaa ctcgcatttt atctttataa tgaggataaa
      841 aaattacata aaatgaaaaa tatagttttt aaactttatg tcttaaaata gcattgaatt
      901 atttatataa aaccaacttc gaccataaaa aacaacaagg caacagttaa actaatttaa
      961 aactaggcgc taaatatttc attcaccatc gtatatttgc aacacaaaaa caacaaaaac
     1021 acaattgaaa gctaaagaat taatccataa aattattccg gctacgctgg ctttatacgc
     1081 atctttacta gtggttcatg tctatcaaat tatcatgcaa cctagtcagt atttagggca
     1141 gcctgtgttg tttaaatacc acctaaaagg ttttgtgatg gatttagaat tcacattcat
     1201 ttttggtact gttgtgctag cactttgtct tttattctat aaattacttg ggaaatacac
     1261 ggttgtttta attcacttac tcaatgtcat tgctcttttt gcaaacatca gtttgatttt
     1321 atatttttta gaagccaatc aaccattggg aaaagtgttt tttgaacttt cttctacaga
     1381 attgaaaatg gcatccaacc cagaatcgtt tttaaatatt gttgttttac ttgctcttgt
     1441 tggaattatt actttttata ttctaactta ttacctattt cgtaaataca gtgaacataa
     1501 atggctgaaa attggtgttg taacactttg gctgtttagt ttagttgcaa ttccgtggag
     1561 catattagag tcaaaagaca aagtaggcga tatggttgct aataacaaag gtttatactt
     1621 cttgaacgag gctcgtcagt atttttttaa tactgatgct tctaattttc catttacaga
     1681 aaaagatttg agattaatgg atgcttcatt ttttaataac aggcacccag aagatataaa
     1741 acatcctttt ttacacgatt tcaccaatga gagtagtctg aatgaactac ttcatcacga
     1801 tagtaaacgc cctcccaata ttgtttacat tatagtagaa agtttatcca accacatgtt
     1861 tggagaggga gctgatgtat cgggt
//
LOCUS       DAOQVQ010000477         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_80070_length_1879_cov_4.896930, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000477 DAOQVQ010000000
VERSION     DAOQVQ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80070_length_1879_cov_4.896930"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..578
                     /gene="ssrA"
                     /locus_tag="PKN22_11080"
     tmRNA           175..578
                     /gene="ssrA"
                     /locus_tag="PKN22_11080"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1084..>1879
                     /locus_tag="PKN22_11085"
     CDS             1084..>1879
                     /locus_tag="PKN22_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43292.1"
                     /translation="MKFLVALFFAFWNFLAICQVDFSIIEQKKLEGDFEAARSKVEFY
                     LQQKNTTFNQKVQLFQTNGDLAMLSGDFDFALENWKKAAQLRSKIYPNKKDYHHALTY
                     ASFSNYYYEKYDKILSKKYADSCSRLLNGLTLNQQLELEIFKIWNILGQSYKQSASAS
                     DYQKFSKIYAKTIDFYKKSVDFIVKNKLNKHYLAKSLHLLGNAYFDWTYAEFEAGNKE
                     KVDFTQKKAIAYYDQAISLWQQLYGNQHYELGRTYFIKALLFSNLPI"
BASE COUNT          612 a          300 c          342 g          625 t
ORIGIN      
        1 tcctaaaatc ccagtaaaac cccacacaaa aatgatgagg tgcattaaga tatggtattg
       61 gtattttttt gtcatattgg cactttcggt tgcgaaatta cagaaaggga ttgtttttta
      121 ttcgttaaat ctttactttt ttaagtattc cgttgtatct ttgtagggaa attcggggtc
      181 gtattggatt tgacagcaga tgcggcgact gagtgtaagc atgtcgagcg ttgtggtgta
      241 gctcgttaat ctcatgtctc aaacaataaa tgacaacacg tcatacgcac ttgctgctta
      301 attaacggaa tgttaataga gcttaatctg accgaagtac agtaggttag acaagtacct
      361 tctctcttgt tttagcttga agaacagaga atacggaggt tcatcctttc aagctggcac
      421 caaatttact tcgtaggcgg tgtgagattt taagaagtta agcgttactc tgggtgttct
      481 tgtccaattt aacgtcgaaa attaatcacg aactaaacat gtagaagcct taggagttct
      541 ttgtttggac gagggttcga aaccctccga ctccactctg ataaaagcat caccttgaaa
      601 agaggcgatg cttttttggt ttataccgac ttaagtttac tttaaggagg aagaaagcta
      661 aaaagtatca atttttcgta agaaaaatag gcttttatcc actgcacgtt atttaaaggg
      721 ttcataaaat aaaccctccg accttttcaa tagaaccata attttcctaa ttgtgtgcat
      781 ttttttattt ttttgcacac aaaaacgtgt tataagttgg ttgtatttgt tggtgctttt
      841 tattacattt accccaaaat gctgcaattt tataaaacta acagctattt gaagaattga
      901 tggaattaat taactcgctt caaaattgat gtaaaaatta tgcttaaagt gccttttgca
      961 aatcagaaat atagaacagt ttcttttagt gctattgaac gtagcaagtc caattcaatt
     1021 gttcttttat tgaaacactc aagcaatcag gtcgttgtac atcattcaat tttgtaattt
     1081 gcgatgaaat ttcttgtagc ccttttcttt gctttttgga atttcttggc gatttgccag
     1141 gttgattttt caattattga gcaaaaaaag ttggaaggtg attttgaagc agctcgaagt
     1201 aaagtggaat tctaccttca gcaaaaaaat actacgttca accaaaaagt ccaattattt
     1261 caaactaatg gtgatttggc catgttgagc ggtgactttg actttgcact tgaaaattgg
     1321 aaaaaagcgg cacaattacg atcaaagatt tatcccaata aaaaagatta ccatcatgca
     1381 ttgacctacg caagttttag caattattat tacgaaaagt acgataaaat tctttcaaaa
     1441 aaatatgctg atagttgctc aaggttatta aatgggctaa cgctcaacca acaattggag
     1501 ttggaaattt ttaaaatttg gaacatttta gggcaatcct acaaacaaag tgcaagtgct
     1561 tcggattatc aaaaattttc taagatttat gcaaaaacaa tcgattttta taaaaaatct
     1621 gtcgatttta tagttaaaaa caagctaaac aagcattatt tagcaaaatc acttcatttg
     1681 ttgggaaatg cctattttga ttggacctat gcagaatttg aagctgggaa taaggaaaaa
     1741 gttgatttca cacaaaaaaa agcaatagcc tattacgacc aagctatttc tctttggcag
     1801 caattgtatg gaaatcagca ctatgagttg ggacgaacgt attttattaa agcattgtta
     1861 tttagcaatt tacccatag
//
LOCUS       DAOQVQ010000478         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_80432_length_1874_cov_3.379329, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000478 DAOQVQ010000000
VERSION     DAOQVQ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80432_length_1874_cov_3.379329"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..566
                     /locus_tag="PKN22_11090"
     CDS             123..566
                     /locus_tag="PKN22_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43293.1"
                     /translation="MKFFLLLLLVAVGFSKSDFYTVIASNDQEKVVALEKKLASLSES
                     NDQQAYWGAIQMKSSYFLKTPAEKLKKFKSGHQLLEKIIAQHPNNAEYRFLRLIIQEN
                     APKVVKYNANIAEDAAFIKKNIATVTSEVKKEMVAYAKISENLNL"
     gene            693..1697
                     /locus_tag="PKN22_11095"
     CDS             693..1697
                     /locus_tag="PKN22_11095"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS43294.1"
                     /translation="MILVTGATGLLGSHLLVELLKSDRKVRALYRDKQKIKLVEQLFD
                     YYFQGTEHRFNQIEWMQADVLDLVSLDDAFQGITEVYHCAALVSFNRKDFKNMIHVNR
                     LGTANVVNFALKYGVKKLGHVSSTAVFTAKENGEVTEQTKWQISDTTSGYAISKYNSE
                     REVWRATEEGMDVIIINPSVIFGAGNLSESSLAIFKTVKNGLKVYSPGCNAFVDARDV
                     AHCFVQLMESDVKNERFLCIGHNMSFKESIDTIATAFGKKTPTICPPKWIALLMGRTN
                     ELLNRLFGIKPTITLESARSAYSSTRYSNSKIVEQTNHTFFSFQETVNNVVAFDKTRS
                     "
BASE COUNT          642 a          297 c          356 g          579 t
ORIGIN      
        1 ataaccgaga aatgattgaa gaattaaaga aaaagcgcaa aaaattattt gaattgcttt
       61 ttgaagatta attttggaat agtaattggg actattgcat acgttttatt taataggaac
      121 acatgaaatt ttttcttctt ttattgttgg tagccgttgg attttcaaag tctgattttt
      181 atactgttat tgcatcaaat gaccaagaaa aagttgttgc attggagaaa aaattagcca
      241 gcttatccga aagtaacgat caacaagcat attggggtgc cattcaaatg aaaagttcct
      301 attttttaaa aacaccagcc gaaaagctta aaaaatttaa aagcggtcac caattattag
      361 aaaaaataat tgcccaacac cccaacaatg cagaatatcg gtttttgcgt ttaattattc
      421 aagaaaatgc gccaaaagtg gtaaaataca acgccaacat tgccgaagat gctgcattta
      481 tcaaaaagaa cattgcaaca gtaacaagcg aagtaaaaaa agaaatggtt gcttatgcaa
      541 aaatttctga gaatttgaat ttgtaaatca attaatcaac aatacgactg aacttcaaat
      601 tttaaatacc tctttatttt caaaaaaggg cgttcatcaa taaaaaacag ttcaacttta
      661 gcagatgtta aaattgaacg tatctttata ccatgatttt ggttactggt gcaacaggtc
      721 ttttgggtag tcacctactc gttgagttac ttaaaagtga ccgaaaagtg cgtgcattat
      781 accgcgacaa acaaaaaatc aagttggtag agcaattgtt tgactactat ttccaaggca
      841 cggaacatcg tttcaatcaa atagaatgga tgcaagcaga tgtgttggat ttggtaagtt
      901 tagacgatgc gtttcaagga attaccgaag tttatcattg cgctgctttg gtttcattta
      961 accgtaaaga tttcaaaaac atgattcacg tcaaccgttt gggaactgcc aatgtggtga
     1021 attttgcatt gaaatatgga gtaaaaaaat tgggacatgt tagctcaacc gccgttttta
     1081 ctgccaaaga aaacggtgaa gttaccgagc aaaccaagtg gcaaatttcg gatacaacaa
     1141 gtggatatgc tattagtaaa tacaattcgg agcgtgaagt gtggcgagca acagaagaag
     1201 gcatggatgt tatcattatc aatccatcgg tgatttttgg tgcaggcaat ttatccgaaa
     1261 gttcgttggc tatttttaaa acggtaaaaa acggtttgaa agtttattca cccggttgca
     1321 acgcctttgt tgatgcacgc gatgtggccc attgttttgt gcaactaatg gaaagcgatg
     1381 ttaaaaatga acgcttttta tgcattgggc acaacatgtc gtttaaagaa tcgattgata
     1441 caattgcaac agcttttggt aaaaaaacac ctacaatttg tcccccaaaa tggatagcac
     1501 ttttaatggg gcgcaccaat gaattgttga atcgtttatt tggaataaaa cccaccatta
     1561 cattagaaag tgctcgctcg gcctattctt caacacgcta ttccaattca aaaattgtgg
     1621 agcaaaccaa tcataccttt ttttctttcc aagaaactgt caacaacgtg gtagcgtttg
     1681 ataaaacacg aagttagttt tcattgtaga aaatgaatga gctaaagaaa attttcataa
     1741 ttaattctta tcttaacccc tttgaatttc aagtaaatgt ttattatagg tttaattata
     1801 gctgtttttg taggtattac aatggggcta attggtggtg gaggtagcgt tattactgtt
     1861 ccaatgttgg tata
//
LOCUS       DAOQVQ010000479         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_80832_length_1868_cov_4.004413, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000479 DAOQVQ010000000
VERSION     DAOQVQ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80832_length_1868_cov_4.004413"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKN22_11100"
     CDS             <1..653
                     /locus_tag="PKN22_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS43295.1"
                     /translation="GINKDLFFELKDALKGADYNLQYVPSQKLDSITRNNHQGVIAFV
                     SPVEYGNIEQLVDEKLEAGEKVNILVLDRITDVRNFGGIARTAECQGVDAILIPSKGS
                     ALVTSDAVKTSAGALNRIKVCKTDDLKNSLFYLQQSGVRLIACTEKSKNNLFETNLRG
                     SVAIIMGSEEDGISSDLIKMSDISTKVPMIGEIASLNVGVAAGMILYEKTRQELYG"
     gene            663..1403
                     /locus_tag="PKN22_11105"
     CDS             663..1403
                     /locus_tag="PKN22_11105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNS43296.1"
                     /translation="MKLYLNETTYFDTSEGIDISIPIGEPQHGARAWYVDYPVIEPVR
                     ANGYIGSVEEGGSVNFRNIFFNPHGNGTHTECLGHITSEVFSINTYLKEFFFEALLVT
                     VQPEKQTNGDWVITQNILEKSVGNTTAQALVVRTLPNDLNKLSVDYSTTNPPYFDVNC
                     IEILDRMGVEHLLIDLPSVDRESDNGVLAMHHAFWQVPENPQYHKTITEFVFVPSNVL
                     DGRYVLNLQVAPFVNDASPSRPVLYALK"
BASE COUNT          603 a          302 c          348 g          615 t
ORIGIN      
        1 aaggaatcaa caaagacctg ttttttgaat taaaagatgc tttgaaaggg gcagattata
       61 atttgcaata cgttccttcg cagaaattag acagcattac acgcaacaac caccaaggag
      121 ttatagcctt tgtttcgcca gtagaatatg gtaacatcga acaattggta gatgaaaaat
      181 tagaagcagg cgaaaaagta aacattttgg tgttggacag aattaccgat gtgcgtaatt
      241 ttggaggtat tgctcgtact gctgaatgcc aaggtgtgga tgccatctta attccatcaa
      301 aaggttcggc gttggtaact tcggatgccg taaaaacttc ggcaggagca ttgaatcgta
      361 tcaaggtatg caaaaccgat gatttaaaaa attcgttatt ttatttgcaa caaagtggtg
      421 ttcgattaat tgcttgtacc gaaaaatcaa aaaacaattt gtttgaaact aatttacgtg
      481 gttcggttgc catcatcatg gggtcagaag aggatggaat tagctctgat ttaattaaaa
      541 tgagcgatat ttctaccaaa gtaccaatga ttggtgaaat tgcatcgtta aacgttggtg
      601 ttgctgcagg aatgatttta tacgaaaaaa cacgtcaaga attatacgga taaacgtaga
      661 ttatgaaatt atacttaaac gaaactactt attttgatac ttcagaaggc attgatattt
      721 ctatacctat tggtgagcct caacacggcg cgcgtgcatg gtatgtggat taccctgtga
      781 ttgagcctgt acgagccaac ggctatattg gaagtgtgga agagggtgga agtgtcaatt
      841 ttagaaatat tttcttcaat ccgcatggaa acggcacgca tactgaatgt ttgggacata
      901 taacatcgga agtattttct atcaacacct atttaaaaga attttttttc gaagctttgt
      961 tggtgactgt tcaaccagaa aaacaaacca acggcgattg ggttattaca caaaatattt
     1021 tagaaaaaag cgttggaaac actactgcac aagccttggt agtgcgcacg ttgcccaatg
     1081 atttgaataa attatccgtt gattattcca ccaccaatcc tccttatttt gatgtaaatt
     1141 gtatcgaaat tttggataga atgggcgtag agcatttgtt gattgacctc ccatccgttg
     1201 atagagagtc agacaatgga gttttggcaa tgcaccacgc attttggcaa gtgcccgaaa
     1261 accctcaata ccacaaaaca atcaccgaat ttgtttttgt tccctctaat gtgttggatg
     1321 gaagatatgt gctcaatctt caagttgctc cttttgtaaa tgatgctagt ccaagtagac
     1381 cagtattgta tgcattaaaa taaaaaaaag gttgaacttt caaccttttt ttagtactag
     1441 tagtagtatc tctttaattt agtaaatagt tttcatctaa cttagtaaaa tagttttctt
     1501 tattgtagta tagttatatc tttaattttt taatctcttt ctttttaaat cagttacttc
     1561 ttacgtcctc cgtatcatga aaataaattg aggattttag aactgttata ctctttaatc
     1621 aaactagaaa atgaaacctg cctctccaag cagtacccca atttagtatt tcaaagcgaa
     1681 cgtaagaagc gacctgattt atttctcctt atttctgata ctaaagtagg tgttttattt
     1741 taattaaatt tttcaaatga atattcatag tctataaatg aatattttgc ctttttcaat
     1801 gaatgtttgt tttttttgac tagtcctaaa taaccgatac agattattaa gacaaaaata
     1861 attaatta
//
LOCUS       DAOQVQ010000480         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_80929_length_1867_cov_2.683775, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000480 DAOQVQ010000000
VERSION     DAOQVQ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80929_length_1867_cov_2.683775"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKN22_11110"
     CDS             complement(<1..259)
                     /locus_tag="PKN22_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43297.1"
                     /translation="MKIKLTLLSLCLVPVIGLSQYTVDAVRKIANTAPEAQLVVEASR
                     MLQDDYYYFSEIMVDKLLEKNPESSNYNYRKGYIVLNSRSDF"
     gene            complement(285..1628)
                     /locus_tag="PKN22_11115"
     CDS             complement(285..1628)
                     /locus_tag="PKN22_11115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNS43298.1"
                     /translation="MARIEIKLPKMGESVTEATITNWLKNIGDSVELDEPLVEVATDK
                     VDNELPSEAKGVLVERLFEVDQVAQVGDVIAIISTDGGDAPSSSSAPAKAETPVAVAE
                     KIVETAVATASASSADFSSSDKFYSPLVKSIAKAEGISINELDAIAGSGQAGRVTKKD
                     ILAYLDDRKNGAAPKVAAPAPTKVEVVAPKVEAPAGGGFLSNIKEPIVVPNPGDEIME
                     MDRMRKIIAEHMVMSKHVSPHVTSYVEADVTNIVNWRNKVKDAYKKREGENITFTPIF
                     IEAIAKAIKDFPMINVSVSGTQIIKRKNINIGMATALPTGNLIVPVIKNADQLNLTGL
                     SKAVNSLANSARNNQLKPEDIQGGTYTVTNVGTFANVMGTPIINQPQVAILALGAIRK
                     MPAVIETPDGDFIGIRHKMFLSHAYDHRVVDGALGGQFVRRVADYLEQFDPNRDF"
BASE COUNT          544 a          353 c          303 g          667 t
ORIGIN      
        1 gaaaatcaga acgagagttc aaaacaatgt agcctttacg gtaattgtag tttgaactct
       61 caggattttt ttctaataat ttatctacca ttatttcaga aaaataatag taatcatctt
      121 gcaacattct tgaagcctca acaactaact gtgcttcagg agctgtgttc gcaatttttc
      181 ttacagcatc aactgtatat tgtgacagcc caattactgg aactaggcag agtgataata
      241 gggttaattt aattttcatt acttaatttt taaaatttag tttattaaaa gtctctattt
      301 gggtcaaatt gctctaaata atcagcaaca cgtcgcacaa attgtccacc caatgctcca
      361 tcaactacac ggtggtcata cgcatgagat aagaacattt tgtgacgaat tccaatgaaa
      421 tcaccatctg gcgtttcaat tacagcaggc atttttctaa tagcacctaa tgctaaaatt
      481 gcaacttgtg gttgattgat aattggagtt cccataacgt ttgcaaaagt accaacgttg
      541 gttactgtat aagttcctcc ttgtatatct tctggtttta attggttgtt acgtgcactg
      601 ttggccaatg aatttaccgc ttttgacaag ccagttaagt tcaattggtc ggcattttta
      661 attactggaa caattaagtt acccgtaggc aatgcggttg ccataccaat attaatattt
      721 tttcttttga tgatttgtgt accacttacc gatacgttaa tcattgggaa atctttgatt
      781 gcttttgcaa ttgcttcaat gaaaattggc gtaaatgtaa tgttttcacc ttcgcgtttt
      841 ttatacgcat ctttcacttt gtttctccaa ttaacaatat ttgtaacgtc tgcttcaaca
      901 taagatgtaa cgtgaggaga aacatgctta gacatcacca tgtgctcagc aataatctta
      961 cgcatacgat ccatctccat gatttcatct cctggattag gaactacaat tggctcttta
     1021 atgttagata agaaaccacc tccagctgga gcttcaactt tgggtgcaac aacttcaact
     1081 tttgttggtg caggtgcagc cacttttgga gccgctccat tttttctatc atctaaatag
     1141 gcaagaatat cttttttagt tactcttcca gcttgtccag aacccgcaat tgcatccaat
     1201 tcattgattg aaataccttc tgctttggca atacttttta caagtggaga ataaaatttg
     1261 tctgatgaac taaaatctgc agatgatgca gaagcagttg caacagctgt ttcaacgatt
     1321 ttttcggcaa cagcaactgg tgtttcagct tttgcaggag cagaactaga gctaggcgca
     1381 tctccaccat ctgttgaaat aatagcaata acatccccaa cttgagcaac ttgatccact
     1441 tcaaataaac gctcaacaag cactcctttt gcttcagaag gtaactcatt gtccaccttg
     1501 tctgtagcaa cttcaactaa tggttcgtct aattcaacac tgtcaccaat gttttttaac
     1561 caatttgtaa tagttgcttc ggttacactt tcacccattt ttgggagctt tatttcaatt
     1621 cttgccattt taaaaatcgt atatgaaatt atgttatcga ctgcaaaaat aattgatttt
     1681 tcctttttaa caaatatatg ttaatttggt tggtccattt ttttccaatt ctttaaaagt
     1741 agttgtaaat cctttttcac tgaatagtct cttgcataaa ttaaatttag tttaattaat
     1801 tgattttctg atagttgatt ggtttttgtg gttagagata aaattccttg tttaatttta
     1861 ggtaatt
//
LOCUS       DAOQVQ010000481         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_80972_length_1866_cov_4.172833, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000481 DAOQVQ010000000
VERSION     DAOQVQ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80972_length_1866_cov_4.172833"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1616
                     /locus_tag="PKN22_11120"
     CDS             168..1616
                     /locus_tag="PKN22_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISFba3 family transposase"
                     /protein_id="HNS43299.1"
                     /translation="MLLKQQTIQFSAHSTLYDLIVPKENILRKINDLIDFSFIYDELL
                     NKYCTTNGRMAESPVRMFKYLLLKTIYTISDVDVVERSQYDMSFKYFLDMNPEDDVIN
                     PSSLTKFRKLRLKDTDLLNLLINKTVTIAIEKGIIRSKSIIVDATHTLSRSNPFLAID
                     VLRERSKLLRKTIYTFDDQFKERMPQKNTDNDLEKELVYCKELEKRIENEPSISSIPA
                     VKEKLNLLKETVEDTQENLTLSKDTDAKIGHKSAESSFFGYKTHLAMTEERIITAAVV
                     TSGEKGDGPELPKLIEISQENGIDVDTIIGDAAYSGKENLKITSEQNIKVVAKLNPSI
                     TQGNRKDADKFDYNKDADRFVCPAGHLAIKKARGGSKNIGTNQVDTYYFDVEKCKSCP
                     LKNGCYKDGAKTKTYSVSIKSELHKDQTAFQETEYYKEKAKHRYKIEAKNSELKNVHG
                     YDKAISYGITNMQMQGAIAIFTVNLKRILKLM"
BASE COUNT          734 a          277 c          301 g          554 t
ORIGIN      
        1 agagagcaat tctaattttt agaattatca tataaaaaaa tcccgattca atgaagaggg
       61 cactgaaaaa gttctttaaa taataattgc aataatttaa ggagtagaaa acttagtttt
      121 tctgctcctt ttttcgtata tttagcttta attatagagt tttttagatg ttattaaagc
      181 agcaaacaat acaatttagt gctcattcta cgttgtatga tttaatagtt ccaaaggaaa
      241 acatattaag aaaaatcaat gatttaattg atttttcctt tatttatgat gagttgctaa
      301 acaaatactg cacaacaaat ggtcgtatgg cagaaagtcc tgttcgtatg tttaagtatt
      361 tacttcttaa aacaatttac actatttccg atgtagatgt ggtggaacgt tctcaatatg
      421 atatgtcttt taaatatttt ttggacatga atcctgaaga cgatgttatc aatccaagtt
      481 cgttgacaaa gttcagaaaa ctacgtttaa aagacaccga cttattaaat ctattgataa
      541 acaaaacagt aactattgct attgaaaaag gaataattcg ttcgaagtct attatcgttg
      601 atgccactca tactttatca agatctaatc cgtttttagc cattgatgta ttgagagaac
      661 gctccaagtt acttcgaaaa acaatataca cctttgacga ccaatttaaa gaacgtatgc
      721 ctcaaaagaa taccgacaat gatttagaaa aggagctggt ttattgtaaa gaattagaaa
      781 aacgtatcga aaatgagcca tctataagtt cgattcctgc agttaaagag aaattaaacc
      841 tgttaaagga aactgtggag gatactcaag aaaacctaac tttatcaaaa gataccgatg
      901 caaaaatagg tcataaatct gctgagagtt ccttttttgg atacaaaact catttagcaa
      961 tgactgaaga gcgcatcatt actgcagccg tagtaacatc gggtgaaaaa ggtgatggac
     1021 cagaattgcc taagcttata gaaattagtc aagaaaacgg aattgatgta gacaccatta
     1081 ttggtgatgc tgcttactct ggaaaagaaa atcttaaaat cacaagtgaa caaaatatta
     1141 aagtagtagc aaaattaaat ccatcaataa cacaaggaaa cagaaaagat gccgataaat
     1201 ttgattataa taaagatgcc gataggtttg tttgcccagc aggacatttg gcaataaaaa
     1261 aagcacgtgg tggaagtaaa aatattggaa caaatcaagt agacacttat tattttgatg
     1321 tcgaaaaatg caaatcttgt cctttaaaaa atggttgtta caaagatgga gctaaaacta
     1381 aaacctattc agtatccata aaatcagaat tacataaaga tcaaacggct tttcaagaaa
     1441 cagaatatta caaggaaaaa gcaaaacatc gatacaagat agaagccaaa aatagcgaat
     1501 taaaaaatgt acacggttat gataaagcaa tttcatacgg tattaccaat atgcaaatgc
     1561 aaggtgcaat agcaatattc acagtaaact taaaaagaat tctcaaatta atgtagaaaa
     1621 cgcaatctgc tcaggtattt tgaaaaaaca acctgtaaaa gccaaaaaca aacttataca
     1681 acctacaata aaaaacagtc gcaaaaagaa aaacgcttat aacggatgtt taaaatcctg
     1741 ttacaagcgt ttttttattg actaaaaaag gcactaatca aaatagtcga tgtttttcag
     1801 tgccctcaat taatttggga ctttttttat attacttttt taataactct tagtttgtgc
     1861 gtatga
//
LOCUS       DAOQVQ010000482         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_81065_length_1865_cov_3.190608, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000482 DAOQVQ010000000
VERSION     DAOQVQ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81065_length_1865_cov_3.190608"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1033)
                     /gene="sucD"
                     /locus_tag="PKN22_11125"
     CDS             complement(152..1033)
                     /gene="sucD"
                     /locus_tag="PKN22_11125"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNS43300.1"
                     /translation="MSVLVNKNSKVIVQGFTGNEGTFHASQMIEYGTNVVGGVTPGKG
                     GTLHLDKPVFNTVKDAVDTTGADVSIIFVPPAFAADAIMEAANAGIKVIVCITEGIPV
                     KDMVQAKEYIKDFDCRLIGPNCPGVITAGEAKVGIMPGFVFKKGKVGIVSKSGTLTYE
                     AADQVAKAGLGITTAIGIGGDPIIGTTTREAVELLMNDPETEGIVMIGEIGGNLEAIA
                     ARWIKESGNKKPVVGFIAGETAPKGRTMGHAGAIVGGADDTAEAKKRIMRECGIHVVD
                     SPAHIGQKMAEVLGVLA"
     gene            complement(1150..>1865)
                     /locus_tag="PKN22_11130"
     CDS             complement(1150..>1865)
                     /locus_tag="PKN22_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43301.1"
                     /translation="GYEALAVEFATDNSFSKMTFNTTQKMQLAQWKKDYWIPEMNQLI
                     QKKSNFNLVKNEILTEENKWDNIKCENCFTNHQLNKENIYRFLSILQLQYTNNFNLAN
                     DSLDVFKHQFGLKKYNQYREKFSNEKLEWLVTSRERLRPLYINRKENKIQQLKNPIYQ
                     QSNNVRFFDSPYFIKEKSFLGFKIDTFFANITVIWLFSLLLYFALHIEFFKRSFTGYK
                     FVLQYLKHSKAFQYINKKH"
BASE COUNT          558 a          337 c          297 g          673 t
ORIGIN      
        1 acgtaaatac atactaaaat ggttgttttc aagttgggaa caaccatttt ttttcgctat
       61 ctttttatag attgattaaa gaagttcccc caaattatgg gtacaaaaaa agtcaccctt
      121 aggaggtgac tttcaattat tttgaaaaat attaagctaa aacgcccaat acttctgcca
      181 ttttttgtcc gatgtgagca ggtgaatcaa ctacgtgaat tccacattct ctcatgatac
      241 gttttttagc ttcagctgta tcgtcagcac caccaacaat tgcacctgcg tgtcccattg
      301 ttcttccttt tggagctgtt tcaccagcga taaaaccaac aactggtttt ttattgccac
      361 tttctttaat ccatcgagca gcaattgctt ccaagttacc accaatttca ccgatcataa
      421 cgataccttc agtttcaggg tcgttcatca ataattcaac tgcttcacga gttgtagttc
      481 caataattgg gtcccccccg ataccgatag ctgttgtaat tcccaatcct gcttttgcaa
      541 cttggtcagc agcttcgtat gttaatgttc cagatttaga aacaatacct acttttcctt
      601 ttttgaaaac aaatcctggc atgataccaa ctttagcttc accagcagtg ataacacctg
      661 gacagttagg accaatcaag cggcaatcaa aatctttgat gtattctttt gcttgcacca
      721 tgtctttaac tggaatacct tctgtaatac aaacgattac tttgatacca gcattcgctg
      781 cttccataat ggcatctgct gcaaatgcag gtggaacaaa aataatagaa acatcagcac
      841 ctgtagtgtc aactgcgtct ttaacagtgt tgaaaacggg tttgtctaag tgcaatgtgc
      901 caccttttcc tggagttaca ccaccaacaa cgtttgttcc atattcaatc atttggcttg
      961 catggaaagt tccttcattt cctgtaaatc cttgtactat tacttttgaa tttttgttaa
     1021 ctaatacgct catatttgta gctattttat atcttgattt ttaaaattgt tcaaatttac
     1081 atttttgaaa tggtttagac aattttatgg gggattattt tacagaataa cgtccatttt
     1141 gttgaaaaat taatgtttct tattaatgta ttgaaatgct ttagaatgct ttaaatattg
     1201 taaaacaaat ttatagcctg taaatgagcg tttaaaaaac tcaatgtgaa gtgcaaaata
     1261 aagcaaaaga ctaaaaagcc aaattactgt aatgttggca aaaaaggtgt cgattttaaa
     1321 ccctaaaaat gatttttctt taataaaata gggggagtca aaaaaacgaa cattgttact
     1381 ttgttggtat atcgggtttt ttaattgttg aattttgttt tccttgcgat tgatgtaaag
     1441 tggccttagt cgctcgcgag aagttacaag ccactccaat ttttcattac taaatttttc
     1501 tctatattga ttgtattttt tcaatccaaa ttgatgcttg aaaacatcca acgaatcgtt
     1561 tgccaaattg aaattattgg tgtattgcaa ttgtaaaatg gataaaaaac gatatatgtt
     1621 ttctttgttc aattgatggt tggtaaaaca attctcgcat tttatattgt cccatttatt
     1681 ttcttctgta agaatttcat ttttgacaag gttgaaattt gatttctttt gaataagttg
     1741 attcatttcc ggaatccaat aatctttttt ccattgtgca agttgcattt tttgggtagt
     1801 attaaaggtc attttggaaa atgaattgtc tgttgcaaat tctacagcta atgcttcata
     1861 acccc
//
LOCUS       DAOQVQ010000483         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_81346_length_1861_cov_3.249723, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000483 DAOQVQ010000000
VERSION     DAOQVQ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81346_length_1861_cov_3.249723"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKN22_11135"
     CDS             <1..283
                     /locus_tag="PKN22_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43302.1"
                     /translation="TFGIILFHSSMPLKMIVRIGLTMSIVFFGMSCYYVYQKLFNDVE
                     MGFTSIIVAIFLSTGLILVSLGIIGEYIRRIWVRQNQLEQVAISEQWKA"
     gene            271..795
                     /locus_tag="PKN22_11140"
     CDS             271..795
                     /locus_tag="PKN22_11140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS43303.1"
                     /translation="MESISGFGITLKPLTEEWLEIVRQWRNQTNVSQFMEFQATISPS
                     EQLTWFKNITNAYYFIILSDNTPVGLIDVKKIDNVKKTAETGLFIGNQVFVGSGVTLG
                     ASILLLDFAFNTLLLDNVTAKINNNNQAALQYNQFLGFEKTENSSSKFSYWMLRKETY
                     INLRNKLVKIVERT"
     gene            complement(907..>1861)
                     /locus_tag="PKN22_11145"
     CDS             complement(907..>1861)
                     /locus_tag="PKN22_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNS43304.1"
                     /translation="KPQVYKDHNLTINASNLCSEIMLPSTDDESFICCLSSMNLELYD
                     EWKDTEAVKLAIFFLDAVLQEFIEKTEGNHYLSAANKFAKRHRALGLGVLGWHSLLQK
                     RMIPFEGLQSKQLTTEIFSYLHDKTEKATQELAMIYGEPELLKGYGRRNTTTMAIAPT
                     TSSSAILGQTSPGIEPFSSNYYKAGLAKGNFIRKNKYLKALLEAKGIDNEDTWRSIMM
                     NQGSVQHLEELTQDEKDVFKTFKEISQLEIIQQASIRQKYVDQAQSLNINIPSGVPIK
                     EVNRLIIEAWKLGVKTLYYQRSQSVSKEMAVNVVSCTSCEA"
BASE COUNT          562 a          334 c          311 g          654 t
ORIGIN      
        1 aacatttgga atcattttgt ttcattcgtc tatgccattg aaaatgattg ttcgcattgg
       61 gcttacaatg tccattgtgt tttttggcat gagttgctat tatgtctatc aaaaattatt
      121 caacgatgta gagatgggtt ttacctctat cattgttgcc atttttctat caacaggttt
      181 aattttagtg tcgcttggaa ttattggtga gtatattcga cgtatttggg tgcgtcaaaa
      241 tcaattggaa caagttgcaa tatctgaaca atggaaagca taagtgggtt tggaattacc
      301 ttaaaaccgt taaccgaaga atggttagaa atcgttcgac aatggcgcaa tcaaacaaat
      361 gtttctcaat ttatggagtt tcaagcaacc atttcacctt ctgagcaatt aacttggttt
      421 aaaaacatta caaacgccta ctattttatt attctttcag ataatacacc agttgggttg
      481 attgatgtaa aaaaaataga caacgttaaa aaaacagcag aaacaggttt atttattggt
      541 aatcaggtat ttgttggttc gggtgttact ttgggggcat ccattttatt gttggatttt
      601 gcattcaaca cacttttatt ggacaatgtt acggctaaaa taaacaacaa caatcaggca
      661 gctttgcaat acaatcaatt tttagggttt gaaaaaacag agaactcctc gtctaaattt
      721 tcatattgga tgcttcgcaa agaaacatac ataaatttac gcaacaaatt agtaaagata
      781 gttgaacgaa cttaatacaa aggttggtcg ttctactgaa atttcatttc ttaattctct
      841 tacaattcaa caaaaaaaat agcccacaat aattgcaggc tatttcgtac tatatttcag
      901 tgcgaattac gcttcacagc ttgtacagct taccacattt accgccattt ctttagaaac
      961 actttgtgaa cgttgataat acaatgtttt aacacctaac ttccatgctt cgataatcaa
     1021 acggtttact tctttaattg gaacaccaga tggaatgttg atgttcaaac tttgcgcttg
     1081 atctacgtat ttctgacgaa tagatgcttg ttgaatgatt tccaattggc taatttcttt
     1141 aaacgttttg aaaacatctt tttcatcttg tgtcaactct tctaaatgtt gcacactacc
     1201 ttgattcatc atgatgcttc tccatgtgtc ttcattgtcg atgccttttg cttctaacaa
     1261 tgctttcaag tatttatttt tacgaataaa gttacctttt gctaaacctg ctttgtagta
     1321 attggatgaa aacggctcaa ttccaggcga tgtttgccct aaaattgcag aagaagaggt
     1381 ggttggcgca attgccattg ttgttgtatt tcttcttcca taacctttta ataactcagg
     1441 ttcaccataa atcattgcca attcttgtgt tgctttttcg gttttatcgt gtaaatagct
     1501 gaaaatttct gttgtcaact gtttagattg caacccttcg aatggaatca tgcgtttttg
     1561 caacaatgaa tgccagccta atacacctaa tccaagtgca cggtggcgtt tcgcaaattt
     1621 atttgccgca cttaaatagt gatttccttc tgttttttca ataaattctt gcaatacagc
     1681 atctaagaaa aagatagcca atttcactgc ttcagtatct ttccactcgt catacaactc
     1741 caagttcatg gatgaaagac agcaaataaa cgactcgtca tctgtagaag gcaacataat
     1801 ctccgaacac aagttacttg cattaatcgt taaattatgg tctttgtaaa cttgtggttt
     1861 a
//
LOCUS       DAOQVQ010000484         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_81400_length_1860_cov_3.508033, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000484 DAOQVQ010000000
VERSION     DAOQVQ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81400_length_1860_cov_3.508033"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKN22_11150"
     CDS             complement(<1..738)
                     /locus_tag="PKN22_11150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS43305.1"
                     /translation="MKQHYDVLVIGAGPAGSIAAHKLIKEGKSVLVLEKMVFPRFVIG
                     ESLLPHIMDYLDQLDLLEVIGAQKYQVKDGVSFYHNDNKCQFLFNNQVNEDYWKYTWQ
                     VKRADFDNVLIQEAQKRGADVIYEATVTAVTNSFNLQTVEFEHPTMGKQTVTATFLLD
                     ASGYGRVLPRLKDLEVPSTALPRGAVFTHIRDINRTTDAGKNILIHVFRDNKAWFWSI
                     PFSDGSCSVGIVSDVDFIKECAADDAKE"
     gene            complement(746..>1860)
                     /locus_tag="PKN22_11155"
     CDS             complement(746..>1860)
                     /locus_tag="PKN22_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="all-trans-retinol 13,14-reductase"
                     /protein_id="HNS43306.1"
                     /translation="EICTYFPLYNIATSAPKTYHTHPDILAISASDFIDGITKSQELK
                     AAFLGNGLLYAFDKNRTPLHVVALILNSYIKGSYRIENGGSQLTKALVKKIRHYNGEL
                     LKRKEVVKTIVNAEKKITQVVCQDGSSYQATTFISNLHPVVTMDLVGKQHLMPATVQR
                     IASLKNSIASFVVNISLKEGVFPYHNSNYYDFFTPDAWETVEYDKNQWPQLLFSCTPP
                     SSKSATFADTFTSMTYIHWQEFEQWKQTHNSIVAPAERDASYQQFKNELAQKVVQRLI
                     ERFPQLENAIEEVHCSTPLTFRDYLGNLEGEMYGIEKDFNNPIKTIINPKTKISNLYL
                     TGQNIVFHGILGATIGAFVTSFNFVDANKIINSIKE"
BASE COUNT          544 a          357 c          348 g          611 t
ORIGIN      
        1 ttctttggca tcatcggcag cacattcttt gatgaaatca acatcagaaa caatgcccac
       61 agagcacgaa ccatccgaaa atggaatgga ccaaaaccaa gctttgttat ctctaaacac
      121 gtggatgagt atgtttttac cagcatctgt tgtgcggttg atatcacgga tatgtgtaaa
      181 aacagcccct cgaggcaatg ctgtggatgg aacttctaaa tcttttaaac gcggcaaaac
      241 tcttccatag ccacttgcat ccaataagaa cgttgccgta acggtttgtt tgcccattgt
      301 tgggtgctca aattcaacgg tttgcaagtt gaaactattg gttacagcgg ttacagttgc
      361 ttcgtaaatc acatcggctc cacgtttttg cgcttcttga atcaatacgt tgtcaaaatc
      421 agcacgtttt acttgccagg tgtatttcca ataatcttcg ttgacttggt tgttaaacaa
      481 aaactgacat ttgttgtcgt tgtggtaaaa agaaacacca tctttaactt ggtatttttg
      541 tgcaccaatg acctccagta aatccagttg gtctaaataa tccataatgt gtggcaacaa
      601 actctcgcca attacaaatc gcggaaaaac cattttttca agcaccaaaa cgctttttcc
      661 ttctttaatt aatttatgag cagcaataga accagcagga ccagcgccaa ttacaagcac
      721 atcatagtgt tgtttcatgt tcgttttatt ctttaatgga gttaattatc ttatttgcat
      781 caacaaagtt aaaagaagtt acgaatgctc caatagttgc ccctaaaatt ccgtgaaaaa
      841 caatgttttg tcctgttaaa tagaggttag aaattttagt tttagggttt atgatggttt
      901 ttattggatt gttaaaatct ttttcaatgc catacatttc gccttccaaa ttgcccaaat
      961 aatctctaaa tgtgaggggc gtgctgcagt gtacttcttc tatcgcattt tcaagttgcg
     1021 gaaagcgttc aattaaacgt tgaacaactt tttgagccaa ttcattttta aattgttggt
     1081 aggacgcatc tctttctgct ggagcaacaa ttgaattatg ggtttgtttc cattgttcaa
     1141 attcttgcca atgaatgtag gtcatgctgg taaatgtatc ggcaaatgtt gcactttttg
     1201 atgatggagg agtgcaacta aataacaatt gtggccattg atttttatcg tattccacag
     1261 tttcccatgc atctggtgtg aaaaaatcgt agtaattgct gttgtgatac ggaaaaacac
     1321 cttctttcaa tgaaatgttt accacaaaag aagcaatgga gtttttaagc gatgcaatgc
     1381 gctgaaccgt tgcaggcatt aaatgctgtt ttccaactaa atccatcgta acaacgggat
     1441 gcagattgga aataaacgtt gtggcttgat aactggatcc gtcttgacaa acaacctgtg
     1501 ttatcttttt ttcagcattg acaatggttt ttaccacttc ttttcgtttc aacaactcac
     1561 cgttgtagtg acgaattttt ttaaccaacg cttttgttag ctgacttcca ccattttcta
     1621 tgcggtaact tcctttgatg taactgttta atatcaacgc aacaacgtgc aaaggagttc
     1681 ggtttttatc aaaggcataa agtaaaccat tacccaaaaa tgcagccttt aactcttgac
     1741 ttttagtaat gccatctata aaatccgaag cactgatagc taaaatgtcg ggatgcgtgt
     1801 gataggtttt tggtgccgat gtagcaatgt tatacaacgg aaaatacgtg caaatttctt
//
LOCUS       DAOQVQ010000485         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_82122_length_1850_cov_2.961003, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000485 DAOQVQ010000000
VERSION     DAOQVQ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82122_length_1850_cov_2.961003"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKN22_11160"
     CDS             <1..447
                     /locus_tag="PKN22_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNS43307.1"
                     /translation="EYSEKHQKQNDTVTAIHSVAELCHKRSLIILFTDFLDNPSKTDE
                     LFHALQHLKHNKHEVIVFHVVDKKTEIEFNFENRPYTLVDMETGEKMKVQPAELKANY
                     QEAINRHFNELKMRMMNHKIDFVQVDINKGYDAVLLQYLLKRQKMN"
     gene            complement(444..1103)
                     /locus_tag="PKN22_11165"
     CDS             complement(444..1103)
                     /locus_tag="PKN22_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43308.1"
                     /translation="MHITKYIGFSVLLLALLACKTEVENDETNTVIKQSQSNEFQGIE
                     ELFDSKEFEDTSVVSLLKEINICSDAQVDKSGKLVTPCTAENFKFFSLNDNKPLKDGF
                     ILLVKANVGGVPLRRVLVFERENGALIKVNGFIANVIGKRKNAGTNDDLLLRFIDKIE
                     GSDVYYNCILKWENNKYVFKTVEVIEEPAGNFRGIVKENVKDSVSKEIHQTLVNNNMI
                     F"
     gene            complement(1090..>1850)
                     /locus_tag="PKN22_11170"
     CDS             complement(1090..>1850)
                     /locus_tag="PKN22_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNS43309.1"
                     /translation="NFDTIDVGSFVSPKSIPQMKDTAELLKKIDLQNAASKLLTIVAN
                     ERGAEDAVQFDEVTYLGYPFSVSEHFQLRNTNSTIEESLHRVETIQSIAQRHNKQVVV
                     YLSMGFGNPYGEHWDAETVIKWSEKLYNDFGLTIQALSDTIGCATEASVDYLFKALIP
                     ELPKVEFGAHLHTTKENASKMVAAAYNAGCRRFDGAIKGFGGCPMAKDDLTGNMPTEI
                     MLDWFENQNIVLPIDKNAFINAMNESNSIFYAYH"
BASE COUNT          607 a          356 c          261 g          626 t
ORIGIN      
        1 gaatattcag aaaaacatca aaaacaaaac gacaccgtta cagctataca ttctgtggct
       61 gaattgtgcc ataaacgtag tttgattatt ttgtttaccg attttttaga taatccatcc
      121 aaaacggatg aactttttca tgctttgcaa caccttaaac acaacaaaca cgaagtaatt
      181 gtttttcatg tagttgacaa aaaaacagaa atagaattta actttgaaaa tcgcccgtat
      241 actttagtag atatggaaac tggcgaaaaa atgaaggtgc aacctgcaga attaaaagct
      301 aactatcaag aagcaattaa tcgccatttc aacgagttaa aaatgcgtat gatgaaccat
      361 aagattgatt ttgtgcaagt agatatcaac aaaggttatg acgctgtttt attgcaatat
      421 ttgctcaaac gtcaaaaaat gaattaaaaa atcatgttgt tattcaccaa tgtttgatga
      481 atttctttag aaacagaatc ttttacattt tcttttacaa ttcctctgaa attacccgct
      541 ggttcttcaa taacttcaac tgttttaaaa acatatttat tgttttccca tttcaaaatg
      601 cagttgtaat acacgtccga accttcaatt ttatcaataa aacgcaacaa taaatcgtcg
      661 tttgttccag cgtttttgcg tttaccaatt acgtttgcaa taaacccatt cacctttatt
      721 aaagcaccat tttcacgttc aaatactaaa actcttcgca atggaactcc tccaacattg
      781 gctttcacca ataaaataaa accatctttc aacggtttat tatcgtttaa gctaaagaat
      841 ttaaaatttt cagccgtaca aggagtaacc aatttccctg atttatccac ttgtgcatca
      901 ctacaaatat taatttcttt tagtagcgaa acaacacttg tatcttcaaa ttcttttgaa
      961 tcaaacaact cctcaatacc ttgaaattca ttggattgac tttgtttaat tactgtattt
     1021 gtttcatcgt tttctacttc ggttttacaa gctaacaaag caagtaaaag aactgaaaaa
     1081 ccaatgtatt tagtgatatg cataaaaaat agaatttgat tcgttcattg catttataaa
     1141 cgcattttta tcaattggta acacgatgtt ttgattttca aaccaatcca acataatttc
     1201 tgtgggcata ttacctgtta aatcgtcttt agccattggg caaccaccaa agccttttat
     1261 tgctccatca aatcgtctgc aaccagcatt ataggccgct gccaccattt ttgatgcatt
     1321 ttctttggtc gtgtgtaagt gcgctccaaa ttctactttt ggtaattctg gaatcaatgc
     1381 tttgaataaa taatcaaccg aagcttctgt tgcacagcca attgtgtcgc tcaacgcttg
     1441 aatggttaac ccaaaatcat tatataactt ttcgctccat ttaattacgg tttctgcatc
     1501 ccaatgttca ccatacggat tgccaaatcc catagacaaa tataccacca cctgtttatt
     1561 gtgtctttga gcaattgatt gaattgtctc tacacggtgc aatgattctt caatagtgct
     1621 attggtgttt cttaattgaa aatgttctga aacagaaaac ggataaccca agtaagttac
     1681 ttcatcaaat tgaacagcat cctctgctcc acgctcgttg gcaacaatgg ttaataattt
     1741 tgatgcagca ttttgaaggt caattttttt caaaagttct gctgtatcct tcatttgagg
     1801 tattgatttt ggagatacaa aactaccaac atcaattgta tcaaaattgc
//
LOCUS       DAOQVQ010000486         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_82436_length_1846_cov_2.208822, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000486 DAOQVQ010000000
VERSION     DAOQVQ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82436_length_1846_cov_2.208822"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PKN22_11175"
     CDS             <1..966
                     /locus_tag="PKN22_11175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNS43310.1"
                     /translation="YVMAGSQKNNVYSIEAALKYFVTGAFATGTLLFGIAWLYGATGS
                     FTLTEISAALANGSSSMAYLGMILILAAFLFKVGAAPFHFWSPDVYDGSPAPVTGFMA
                     TVIKLGAFGAFIKLFGSTFSGMSDFWMPALTFLAIMTMFIGNLSAIRQERFKRFLAYS
                     SISHIGYTLITLIALTENSTYNAWFYLTSYGFATIALITIQIAVNDAEDRIEAFKGVG
                     RKNPLIGIAAVTSLFALAGVPPLMGFFGKYIVFVDAIAQHPGLIAIALLNSAIGIYYY
                     LRIVIVFLQKTEDESVEKTPLSFIQSFILLVCLIGMLFGGVFLML"
     gene            1069..>1846
                     /locus_tag="PKN22_11180"
     CDS             1069..>1846
                     /locus_tag="PKN22_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43311.1"
                     /translation="MKRILLSMIALTMCLSFNGFSQQSNKLKPATEPIPKTSERSVDL
                     NTASRSMTCVDTISYPFAKEEVLGTPTYYTFGIWQSDAEAFSQTFYAYSTGLTISAVE
                     IIGRNNSTNGTTSSTVRARIYSVDGLNNPTGAALATGSVSVTSTSVGVYYVNFTTPIT
                     VTSNYAIVVDVINAGGVFDMFVNNNVAGQTYDEGLSRFKSSYAGYGSNGNWIPIPSVT
                     GLGGVYDFEPLVGAVVSYGINTTATATPTTACLGQPVNFSN"
BASE COUNT          537 a          303 c          359 g          647 t
ORIGIN      
        1 tatgtgatgg ctggttcaca aaaaaacaac gtttattcca tcgaagcagc attgaagtat
       61 tttgttacag gtgcttttgc tactggtaca ttgttgtttg gaattgcatg gttgtacgga
      121 gcaacaggtt cttttacatt aacagaaatc agtgcagcat tggcaaatgg atcaagctca
      181 atggcatatt taggaatgat tttaatttta gcagcattct tatttaaagt gggtgccgca
      241 ccgttccatt tttggagtcc agacgtttat gatggttctc cagcgcctgt tacaggtttt
      301 atggcaacag ttatcaaatt aggagctttt ggagcattca tcaaattgtt tggtagcaca
      361 tttagcggaa tgagtgattt ctggatgcca gcattgactt tcttagcaat catgaccatg
      421 tttattggta acttgtcggc tattcgtcaa gagcgtttta aacgtttctt agcctattca
      481 agtatctcgc atataggtta cacactcatt accttgattg cgttgactga aaacagcacc
      541 tataacgctt ggttttattt aacatcttat ggatttgcaa ccattgcgtt gattaccatt
      601 caaattgcag ttaacgatgc tgaagataga attgaggcat ttaaaggagt tggacgtaaa
      661 aatccattaa tcggaattgc ggcagttact tctttgtttg cacttgcggg tgtaccgcca
      721 ttgatgggat tctttggtaa atacattgtt tttgtagatg ccatagcaca acaccctggt
      781 ttaattgcca ttgccttgtt aaactcggca attgggattt actattattt gagaattgtt
      841 atcgtcttct tgcaaaaaac agaagatgaa tcggttgaaa aaacaccact ttctttcatc
      901 caatccttca ttttattggt ttgtttaatc ggaatgttgt ttggaggtgt atttttaatg
      961 ctataaaatt ttaaaaacac atattcaaaa ggagctaatt ttttagctcc ttttgttttt
     1021 attatcaaat agtttagtat atttgataag tttaaatttt actctattat gaaaagaata
     1081 ttactatcta tgattgcact aactatgtgc ttatcattta acggattttc acaacaatcc
     1141 aacaaattaa aacctgcaac agaaccaatt cctaaaacaa gtgaaaggtc tgttgatttg
     1201 aacaccgcat cgcgttcaat gacctgtgtt gataccatta gttatccttt tgctaaagaa
     1261 gaagttttag gtactccaac atattataca tttggtattt ggcaatctga tgcagaagca
     1321 ttttcgcaaa ctttttatgc ttattcaact ggtttaacca tttcagcagt tgagattatt
     1381 ggtaggaata attccacgaa tggaacaaca agttctacag ttagagcgcg tatttattct
     1441 gtagatggat taaataatcc aacaggggca gctttggcaa cggggtctgt atctgtaaca
     1501 tcaacttctg ttggtgtata ttatgtgaat tttacaactc ctataactgt aacatcaaac
     1561 tatgcaattg ttgtggatgt aataaacgct ggtggtgttt ttgacatgtt tgttaataat
     1621 aatgtagctg gtcaaacgta tgacgaaggg ttgtcgcgtt ttaaatcgtc ttatgccgga
     1681 tatggcagta atggcaattg gattcctatt ccgtctgtaa caggattagg aggtgtttat
     1741 gattttgaac cactagtagg agcagtagtt tcgtatggaa ttaatacaac tgcaactgca
     1801 acaccaacta cagcttgttt aggacaacct gtaaattttt caaata
//
LOCUS       DAOQVQ010000487         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_82458_length_1845_cov_3.779330, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000487 DAOQVQ010000000
VERSION     DAOQVQ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82458_length_1845_cov_3.779330"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKN22_11185"
     CDS             complement(<1..494)
                     /locus_tag="PKN22_11185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNS43312.1"
                     /translation="MKIFIQQWSSVLMTLVMLIPNLLAQQTAGIQQVINYSGFDKASI
                     SLFAMDVKTNEIIYDFQGLKLLAPASTVKLFSTAYALDVLGKDYKPTTKLYYQGNVKD
                     SILNGDIWIVGGGDMSLGSKYFSTDNKDAFLHQWAQKVKASGIKEIHGNIYVDASDFG
                     YEGAP"
     gene            577..1758
                     /locus_tag="PKN22_11190"
     CDS             577..1758
                     /locus_tag="PKN22_11190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS43313.1"
                     /translation="MKKIKVLRIINRFNLGGPTYNATFLTKFLGDEFETMLIGGLPEE
                     GEADSLHILEQYGVEPVLLPEMQRIPNLQSDRKAYKKIKQIIQEFQPDIVHTHAAKAG
                     ALGRKAARAAKVPIIVHTYHGHVFSGYFNPLKTSIYKTIERNLAKKSSAIIAISDLQK
                     EELVKKHKICSSEQTQVISLGFDLEKFHANKDEKRVKTRANYNLADNEIAIAITGRLA
                     PIKNHLFFIDVLKEVAFLTKKQLRIFIVGDGTERPIIEQEIATFNWPANIRFEMTSWI
                     KDIDAFNPGMDLICLCSLNEGTPVSLIEAEAANVPVLSTDVGGVKDVVLDKKTGLIVT
                     SNTKNEYKEKLVALIENDQLRNSLSEAGWDFVKDNFHYTRLVKDVRNLYFKLLNENNA
                     I"
BASE COUNT          615 a          318 c          341 g          571 t
ORIGIN      
        1 ggcgcaccct cataaccaaa atcagaagca tctacataaa tatttccgtg aatttctttt
       61 attccgcttg cttttacttt ctgtgcccat tgatgtaaaa aagcatcttt attgtcggtt
      121 gaaaaatatt tgctaccaag cgacatatcg ccaccaccaa caatccaaat atcgccgttt
      181 aaaatgctgt ctttcacatt tccttgataa tacaattttg tggttggttt gtaatctttt
      241 cccaatacgt ctaaagcgta ggcagtagaa aacaatttaa ccgttgaagc aggagctaat
      301 aattttaaac cttggaaatc ataaataatt tcgttggttt tcacgtccat tgcaaacaag
      361 ctaatggatg ctttatcaaa gccagaatag ttaataactt gctgaattcc cgctgtttgt
      421 tgtgctaaca ggttaggaat taacattacc aatgtcatca aaacactgct ccactgttgt
      481 atgaaaattt tcattttatt gtatttctgc tagaaaatta ataattctac actatttttg
      541 tgcaaaagac tacaaattta ttgccattat cagcttgtga aaaaaataaa agtacttcgc
      601 attattaatc gtttcaatct tggaggacca acctacaacg ccacgttttt aacaaaattc
      661 ttgggcgatg agtttgaaac catgctgatt ggtggtttac ccgaagaagg agaagctgat
      721 tctttgcaca tattggaaca atacggtgtt gagccagttt tacttccaga aatgcaacgt
      781 attcctaatt tgcaatcgga tagaaaggcg tataaaaaaa tcaagcagat cattcaagaa
      841 tttcagcccg acattgttca cacacatgcg gcaaaggcgg gtgcattggg aagaaaagca
      901 gcaagagcag caaaagttcc tattattgtg catacctacc atggacatgt gtttagtggc
      961 tatttcaacc cgttgaaaac gtctatttac aaaaccattg aacgcaattt agccaaaaaa
     1021 agtagcgcca ttattgctat ttctgatttg caaaaagaag agttggttaa aaaacacaag
     1081 atttgtagtt ccgaacaaac acaagttatt tcgcttggct ttgatttaga aaaatttcat
     1141 gcgaataagg atgaaaaacg tgtcaaaact agagcaaatt ataatttagc cgacaacgaa
     1201 attgccattg ccataacagg aaggttggca cccattaaaa atcatttgtt ttttattgat
     1261 gtgttaaaag aagtggcatt tttaacaaaa aaacaattgc gcatttttat tgtgggcgat
     1321 ggtaccgagc gacctattat tgaacaagaa attgcaacat ttaattggcc agcaaacatc
     1381 cgatttgaga tgacttcgtg gataaaagat attgatgctt tcaaccctgg aatggacttg
     1441 atttgtttgt gttcgctcaa cgaaggaacg cctgtaagtt tgattgaagc cgaagcagcc
     1501 aatgttccgg tgctttctac cgatgttggt ggtgtaaaag acgtggtttt agacaaaaaa
     1561 acgggtttaa ttgtaacctc aaacaccaaa aatgagtata aagaaaaatt agtagcgtta
     1621 attgagaatg atcaattgcg caacagcttg tcggaagccg gttgggactt tgtaaaagat
     1681 aattttcatt atactcgatt ggtgaaagac gttagaaatt tatactttaa attattgaac
     1741 gaaaataatg ctatctaaaa aagaaattcg atatagttgt ttgctactgg ttttggttgc
     1801 taccatttct agttgtggta tcaattccaa cataatgttc aaaga
//
LOCUS       DAOQVQ010000488         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_82821_length_1840_cov_4.044258, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000488 DAOQVQ010000000
VERSION     DAOQVQ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82821_length_1840_cov_4.044258"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..562)
                     /locus_tag="PKN22_11195"
     CDS             complement(164..562)
                     /locus_tag="PKN22_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43314.1"
                     /translation="MAEGKISIGRVHSNNGEWVEITIKDEASLVEFIEVKVSLENFAK
                     LITGLSYIDCDFQTRGLNLVGKQREYKTVIVACDGWSLKRDEEEAINAVLAPYEVDGW
                     IGRRYDLKNSKNYVGHEGVKVLFERWVEQE"
     gene            complement(579..1181)
                     /locus_tag="PKN22_11200"
     CDS             complement(579..1181)
                     /locus_tag="PKN22_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43315.1"
                     /translation="MYLNGYGVSIDRGEEFERDGAFYVKMNHNQQYQLQILNNTNSDS
                     AFYVEIDGKNVGGWVVNRNQTVYIDRPLNDNGIFTFYRLNTSESYQAGIQNNENTGLI
                     KVTFYPEKIVPVMRTRAGVQSLGFDEPNVKSISRNHGNESFGAGGTGLSGKSDTKYGP
                     VTTKKFDESHGTVLMMRLVERVVNYNQPRELQSIYPKRFV"
     gene            complement(1181..1531)
                     /locus_tag="PKN22_11205"
     CDS             complement(1181..1531)
                     /locus_tag="PKN22_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43316.1"
                     /translation="MQEIYDINEVPDNSTIIAFFEFNYGDEFDLKEQWLCNKEEYITE
                     TKRIEIEAQNSIREFIEIPFDSNQNIRIRKLSAYYFVIVDENDVDVLKKYFNLIANDK
                     ILNRLFEYFNEGEE"
     gene            complement(1656..>1840)
                     /locus_tag="PKN22_11210"
     CDS             complement(1656..>1840)
                     /locus_tag="PKN22_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43317.1"
                     /translation="DFVNLVHENQDKIYLRDKTYYCIVNNFFVGKNSIQELYNYMFEK
                     GYIKIDSWFDSSMIES"
BASE COUNT          618 a          370 c          201 g          651 t
ORIGIN      
        1 taccctgttt cctcttctaa tacgtaaaca attgctccat cttcaaattg agcattataa
       61 taaacaaatc cttcaaagtg gcaaacgtca actgctgaca ttgctttaag tggaagtgaa
      121 gttgaaatta gaaaaacaaa gataaaaatt atagttttca tttttactcc tgttctaccc
      181 aacgttcaaa taaaacctta acaccttcat gcccaacata attttttgaa tttttcaaat
      241 catatcgtct accaatccaa ccatcaactt catagggtgc aagtacggca ttaatcgctt
      301 cttcctcatc tcgttttaaa ctccaaccgt cacaagcaac aataacagtc ttatactcac
      361 gttgtttacc aactaaatta agaccacgag tttggaaatc acaatcaata tacgataatc
      421 ctgtaattaa tttcgcaaag ttttcaagag aaactttaac ttcaatgaat tcaactaaac
      481 ttgcttcatc tttaattgtg atttcaaccc attcaccatt attggaatga acacgaccaa
      541 ttgaaatttt gccttctgcc atacttgcac cttattcttt atacaaagcg tttaggataa
      601 attgactgta attctcgtgg ttgattataa ttaaccactc tttctaccaa acgcatcatt
      661 aaaactgtac catgactttc atcaaatttc ttagttgtca ctggaccgta ctttgtatcg
      721 cttttaccgc ttaaaccagt tccaccagca ccaaaacttt cattgccatg atttcttgaa
      781 atacttttaa cattaggttc atcaaatcct aacgattgta cacctgctct tgttctcatc
      841 acagggacaa ttttttcagg ataaaaagta accttaatca aaccagtatt ttcattattt
      901 tgaattccag cctgatagct ttctgaagta ttcaaacggt agaatgtaaa gataccgtta
      961 tcgtttaatg ggcgatcaat ataaaccgtt tgatttctat taaccaccca accaccaaca
     1021 tttttcccat caatttcaac ataaaacgct gagtcactat ttgtgttgtt gagaatttgt
     1081 aattgatact gttgattatg gttcatttta acataaaatg cgccatcacg ctcaaattct
     1141 tcacctcgat caattgacac accatatcca tttaaataca ttattcttct ccttcgttaa
     1201 aatattcaaa caaacgattt aaaattttat cattagcaat aagattgaaa tactttttca
     1261 aaacatcaac atcgttttca tcaacaatta caaaataata agcacttaat tttctaattc
     1321 taatattttg gttactatca aatggaattt caataaattc tctaatacta ttttgtgctt
     1381 ctatttcaat tcgtttcgtt tctgtgatat attcctcttt attacataac cattgttctt
     1441 tcaaatcaaa ctcatctcca taattgaatt caaagaacgc aatgatggtt gaattatctg
     1501 gaacttcatt aatatcatag atttcttgca tattattcac cttttttact gaaataaaat
     1561 tcagtatccc tatcaggact cgaacctgaa ctaaaaattt agaagatttt tgtgctgatc
     1621 cattacacta tagagacata atttatactt ttacattaac tctcaatcat ggaactatca
     1681 aaccaactat caattttaat atagcctttt tcaaacatgt aattatacaa ttcttggatt
     1741 gagtttttgc caacaaaaaa attatttaca atgcagtaat aagttttatc acgtagataa
     1801 atcttatctt gattctcatg aactagatta acaaaatctg
//
LOCUS       DAOQVQ010000489         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_82912_length_1839_cov_3.315583, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000489 DAOQVQ010000000
VERSION     DAOQVQ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82912_length_1839_cov_3.315583"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKN22_11215"
     CDS             <1..756
                     /locus_tag="PKN22_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNS43318.1"
                     /translation="HYIGRKEYNNEKHTDATWGVLDEYFNPWTARQISKLKEPFLASL
                     FTLSSHHPYFVPEHRKKDVINGPQPLCASISYGDLSLRLFFEEARKQPWFNNTVFFIC
                     ADHTPGTETPFYNLQTQLYQIPMAIYHPRGKLEKGRVHKIFQQVDIYPTVLDLVNIEE
                     KYYSFGNSLFDTSERFSISYLEGNYFFFENDKMLIFSKNQARNLYDFTAKKESMVDIR
                     SKNRQELVQATAKLKAIIQRYNHDLIENKTSIR"
     gene            753..1631
                     /locus_tag="PKN22_11220"
     CDS             753..1631
                     /locus_tag="PKN22_11220"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNS43319.1"
                     /translation="MKAHVRFIINPISGVGQKGTIPTMIEHHLDLEKFNYDIVYSQYR
                     GHASEIARSSAKENIDVVCAVGGDGTVHEVGTALIGSKTKLAILPAGSGNGLARHLNI
                     PLIIEKAIENINDMNILHMDTVLVNDKPFLNTGGYGFDALIAKKFDEGKQRGFMAYVK
                     LVVREFFKYNPVNVSVDVNGQVKTMPVVMCSIANASEFGNGFCVSPKSDVTDGKIELV
                     LLKPFRIWALPLLAFQFFRKRAHKSKFAEIISFENAKIRLSKRMAHYDGEPVDVRNEL
                     HVQVVPKSLFILSGKK"
     gene            1631..>1839
                     /locus_tag="PKN22_11225"
     CDS             1631..>1839
                     /locus_tag="PKN22_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43320.1"
                     /translation="MQFITLDLEQMLQALNKLEATTQPKWGTMNAQRMVEHLTEMLIA
                     SAGNPIEGFNQLLIPEEILPKMQAF"
BASE COUNT          607 a          342 c          364 g          526 t
ORIGIN      
        1 cactacatag gcagaaaaga atacaacaac gaaaaacaca ccgatgcaac gtggggcgtg
       61 ttggacgaat attttaaccc gtggacagca cgtcaaattt ccaagttgaa agaaccattt
      121 ttagcttcgt tgttcacctt atcgtcgcac catccgtatt ttgtgcccga acaccgtaaa
      181 aaagatgtga taaatggtcc acaacccttg tgcgcttcca tttcttatgg cgatttgtcg
      241 ttgcgcctat tttttgaaga agcacgcaag caaccgtggt tcaacaatac cgtgtttttc
      301 atttgtgccg atcatacgcc gggaacagaa acaccatttt ataatttgca aacgcagtta
      361 taccaaattc caatggctat ttatcatcca agaggaaaat tggagaaggg gagggtgcat
      421 aaaatcttcc aacaagtgga tatttaccca accgtactcg atttagtgaa cattgaagaa
      481 aagtattatt cttttggaaa ttctcttttt gatacaagtg aaagattttc aattagttat
      541 ttagaaggaa attatttttt cttcgaaaat gataaaatgt taattttttc taaaaatcag
      601 gcacgaaatt tgtatgattt tactgcgaaa aaagaatcga tggtggacat ccgttcgaaa
      661 aatcgtcaag agcttgtgca agccacagca aaattgaaag cgataattca gcgatacaac
      721 catgatttga ttgaaaacaa aacgtcaata agatgaaagc gcacgtaaga tttatcatca
      781 atcctatttc cggagtcgga caaaaaggaa ctattccaac aatgatcgag catcatttgg
      841 atttagaaaa atttaattac gacattgtgt attcgcaata ccgtggtcac gccagcgaaa
      901 ttgcgcgttc atcagccaaa gaaaacatcg atgtggtgtg tgctgtaggc ggcgatggaa
      961 cggtacacga agtaggtaca gccctcattg gttctaaaac aaaattggca attcttcctg
     1021 ctggttctgg caacggatta gcacgacacc tcaatattcc tttaattata gaaaaagcca
     1081 tagaaaacat caacgacatg aatattttac acatggatac ggtgttggtc aacgataaac
     1141 cttttctcaa cacaggcggt tatggttttg atgctttgat tgccaaaaaa tttgacgaag
     1201 gaaaacaacg tggtttcatg gcgtatgtga aattggtggt gcgcgaattt tttaaataca
     1261 accctgtaaa cgtttcagtg gatgtgaacg gacaagtgaa aacaatgccc gttgtgatgt
     1321 gttccattgc caatgcttct gaatttggaa atggtttttg tgtttctcca aaatcagatg
     1381 taaccgatgg aaaaattgaa ttggttttat taaaaccttt ccgcatttgg gcgctaccat
     1441 tattagcgtt tcagtttttt agaaaacgtg cccacaaatc aaaatttgcc gaaattatta
     1501 gttttgaaaa tgcaaaaatt cgtctttcaa aacgcatggc tcattacgat ggcgaacctg
     1561 tagatgtgcg caacgaatta cacgtacaag tggttccaaa aagtttattc attttatccg
     1621 gaaaaaaata atgcaattta ttacgttaga tttagagcaa atgctacaag cgttgaacaa
     1681 attagaagca acaacgcaac ccaaatgggg aaccatgaat gcacaacgca tggtggaaca
     1741 tttaaccgaa atgctcattg catcggctgg aaaccctatt gaaggtttta atcaattgct
     1801 cattcccgaa gagatattgc ccaaaatgca agcgttttt
//
LOCUS       DAOQVQ010000490         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_83961_length_1825_cov_3.598305, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000490 DAOQVQ010000000
VERSION     DAOQVQ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83961_length_1825_cov_3.598305"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="fbaA"
                     /locus_tag="PKN22_11230"
     CDS             complement(<1..642)
                     /gene="fbaA"
                     /locus_tag="PKN22_11230"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNS43321.1"
                     /translation="MSHTIKPGVATGDEVQKIFQYAKQKGFALPAVNVTSSSTVNAVM
                     ETAAKVNAPVIIQFSNGGGHFFAGKGLKNDNEKAAILGSISGARHVHEMAEAYGATVI
                     LHTDHCAKKLLPWIDGIMEANEKNFAQTGKALYSSHMIDLSEEPIKENIEICKRYLER
                     MSKIGITLEIELGITGGEEDGVDNTGVDSSKLYTQPEEVAYAYEELMKVSDKFT"
     gene            complement(680..1423)
                     /locus_tag="PKN22_11235"
     CDS             complement(680..1423)
                     /locus_tag="PKN22_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNS43322.1"
                     /translation="MKKGFLIALTAGSLLTLSSCDVLQEAADSLENVASTTNSASLTN
                     QEVVSGLKEALSVGITNSVNLTSVVDGFLKNSEIRLPFPPDAIKAKEKAIEWGMSGQV
                     EKFETTLNRAAEEAAKEALPIFKNAITGMSLQDGFAVLKGGNGAATKFLKDNTTAQLV
                     SAFAPKVQAAIEKVKLTEYWNPIVTKFNAAAPLLGLNKANPDLNEYVTKLAIDGLFKM
                     VEKEENKIRENVGARVTDLLKKVFGSLDK"
     assembly_gap    1567..1576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          580 a          345 c          275 g          615 t
ORIGIN      
        1 cgtgaattta tcgcttactt tcatcaattc ttcataagca taagctactt cttctggttg
       61 agtgtataat ttagagctat caacacccgt gttatccact ccatcttctt ctccacctgt
      121 aattcctaac tctatttcca acgtaattcc aattttactc atacgttcca aatagcgttt
      181 acagatttca atattttctt taattggctc ttctgacaag tcaatcatgt gcgatgaata
      241 caaggctttt cctgtttgag caaagttttt ttcatttgct tccatgatac catcaatcca
      301 aggcaataat ttcttagcac aatggtcggt atgcaaaatc accgttgcac cataagcttc
      361 tgccatttcg tgtacgtgac gtgcaccaga aatagagcct aaaattgctg ctttttcgtt
      421 atcatttttc aatcctttac ctgcaaagaa atgtccacca ccattagaaa actgaatgat
      481 aactggtgca tttacttttg cagctgtttc catcacagca tttactgtgc tagagctagt
      541 aacattaact gctggcaatg caaatccttt ttgttttgcg tattgaaaaa tcttttgtac
      601 ttcgtcgcct gttgcaactc ctggttttat agtatgactc ataattttaa taagatgaat
      661 gcaaagataa gaaagatgat tatttatcca atgacccgaa tacttttttc aataaatcag
      721 taacacgagc acctacattt tcgcgaattt tgttttcttc tttttctacc attttaaaca
      781 aaccatcgat tgctaatttg gtaacatact catttaagtc aggattggct ttgtttaaac
      841 ccaataaagg agctgcagca ttaaatttag taacaattgg attccaatat tcggttaatt
      901 tcactttttc aatagctgct tgaacttttg gcgcaaaagc cgaaactaat tgtgcagttg
      961 tgttgtcttt taaaaacttg gtagcagcac catttccacc tttcaaaacg gcaaatccat
     1021 cctgcaaact cattcctgtg atggcatttt taaagatagg taatgcttct tttgccgctt
     1081 cttctgccgc tctatttaac gtggtttcaa acttctcaac ctgaccgctc attccccact
     1141 ctattgcttt ttctttagct ttgatggcat ctggaggaaa gggcaaacgg atttcgctgt
     1201 ttttcaaaaa tccatcaact acagaagtta aattcaccga attggtgatt ccaactgaca
     1261 aagcttcttt caaaccactt accacttctt ggttggttaa cgatgctgaa tttgttgttg
     1321 acgcaacgtt ttcaagtgaa tctgcggctt cttgcaaaac gtcacaagaa gataatgtca
     1381 ataatgaacc tgctgttaaa gcaattaaaa atcctttttt catattttgt ttttatcgag
     1441 gttaagacaa aagtaatacc aaataaaagg agagttgtaa aaaaatgaaa aatagttatt
     1501 cacaagttgt taaattgtag ggaaatgaag agaatgaata cgaaaaaaat taaaaagaaa
     1561 tcttggnnnn nnnnnnattg gagaaattgt attttaaact tatcaagaat aaaaaggaaa
     1621 taagtatcat ttaaaaacta tgtacagaaa aaaaggagta aaattctccc tttttagtgt
     1681 ttttctctga cgttttcata gttaaactcc gcaacccacg cccaaagcgt ttcaaataat
     1741 aaacacaaat caaaattaca aaaaaatatg attttaccaa aaataaaaca ttatttagca
     1801 gattgaaatt atgaatttga agaag
//
LOCUS       DAOQVQ010000491         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_84104_length_1823_cov_4.714367, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000491 DAOQVQ010000000
VERSION     DAOQVQ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84104_length_1823_cov_4.714367"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..789)
                     /locus_tag="PKN22_11240"
     CDS             complement(1..789)
                     /locus_tag="PKN22_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNS43323.1"
                     /translation="MPFPFGAAFFMCEISYFCNMEERIDKLLLQLNLVSSRVRAEEII
                     RKYGVKVNGKLINKTGKKVPVDATIELLSEEIPWVSRGALKLVEALDKWDIQVEGATC
                     MDIGASTGGFTEVLLKNGAKKVFCVDVGKDQLHASLKADERVVNFEKTHVRELTSKQI
                     TEQCAVGVIDVSFISLDKIFPFITSFLQTDAIVIALVKPQFEAGKEHIGKGGIVKDPS
                     IYPSIIERVKAAAKLSQLESVDCIDSPILGGDGNKEFLLLLKKK"
     gene            complement(810..>1823)
                     /locus_tag="PKN22_11245"
     CDS             complement(810..>1823)
                     /locus_tag="PKN22_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS43324.1"
                     /translation="NPLPAINAGQDVTICAEASTTLMASGGVSYSWDNGLGAGASQTV
                     TPTQTTTYQVTGTDINGCVNTDAVTVTVAPIPSLNIDGINLQGCAPVTPTLNNTLADG
                     SVSCKWIFSDGRTIDGCGPINPVFNNYGCYDVTLMVTSAAGCTNTQTIQDYICVYPTP
                     DASFYATPQVIDNYPWEAQMHNYSSNATNYTWYFGDDSAPSNEFEPTHVYSEENAGTF
                     PIMLVASNDVGCVDTAYYSVKVVEPLIIYVPNTFTPDGDQFNNTFFPVITSGFDVYQY
                     EMLIFNRWGELIFETHDTEVGWDGTYNGKMVQDGTYTWKIEIKIKNYDEHKRYVGHVN
                     LVR"
BASE COUNT          548 a          403 c          310 g          562 t
ORIGIN      
        1 ctatttcttt ttcaacaaga gtaaaaattc tttattgcca tctccaccta aaattggcga
       61 gtcgatgcaa tccacgcttt ccaactgact taatttagca gccgctttta cacgttcaat
      121 aatggaaggg taaatggaag gatctttgac tattccacct ttgccaatat gctcttttcc
      181 agcttcaaat tgtggtttca ccaaggctat aacaatggca tcggtttgta aaaaagaggt
      241 aataaaagga aaaattttat cgagcgatat aaacgaaacg tcaatcacac caacagcgca
      301 ttgctcggtg atttgtttag aagttaactc gcgcacatgt gttttttcaa agtttacaac
      361 gcgttcatcg gctttcaacg atgcgtgcaa ttgatctttc cccacatcca cacaaaatac
      421 tttttttgct ccatttttaa gcaaaacttc ggtaaatcca cccgtagatg ctccaatatc
      481 catacaagtt gctccctcca cctggatgtc ccatttatca agtgcttcaa ccaattttaa
      541 agctcctctg gaaacccacg gaatttcttc tgaaagcaat tcaatagtgg catcaacagg
      601 aacttttttc cctgttttat tgattaattt cccgttgacc ttcacaccgt atttgcgaat
      661 aatttcttct gctcttacgc gtgaactaac caaattcaat tgcaataaaa gtttatctat
      721 gcgttcttcc atgttgcaaa aatacgatat ttcacacata aaaaatgccg ctccaaaagg
      781 aaacggcatt ttgatatttt taggagtaac taacgcacca agttgacatg gccaacatag
      841 cgtttgtgtt catcgtaatt tttaatttta atttcgattt tccaggtata ggttccatct
      901 tgcaccattt taccattgta tgttccatcc caaccaactt ctgtatcatg cgtttcaaaa
      961 attaactcac cccaacggtt gaaaatcaac atttcgtatt ggtacacatc aaaaccagag
     1021 gtaattactg ggaagaaggt attgttgaat tgatcaccat ctggtgtaaa ggtatttgga
     1081 acataaataa tcaacggttc caccactttt acagaataat aagctgtatc cacacaccct
     1141 acatcgttag aagccaccaa catgataggg aaagttcctg cattttcttc agaatataca
     1201 tgcgttggct caaattcatt gcttggtgca gaatcatcac caaaatacca cgtataattg
     1261 gttgcattac ttgaataatt gtgcatttgt gcttcccaag gataattgtc aatcacttgc
     1321 ggagttgcat agaaacttgc atcaggtgta ggataaacac aaatgtaatc ttggatagtt
     1381 tgtgtgtttg tacaacccgc agcagaggtt accatcaatg ttacatcata acaaccatag
     1441 ttgttaaaca ccggattgat tggtccacag ccatcaattg tacggccgtc actaaaaatc
     1501 catttgcaac ttactgaacc atcagccaat gtattgttca atgttggtgt aacaggcgca
     1561 caaccttgca agttaattcc gtcaatgttt aacgatggaa taggagcaac cgtaacagta
     1621 acggcatccg tgttcacaca accgttgatg tctgttccag tcacttgata agttgtagtt
     1681 tgcgttggag taactgtttg agaagcccca gcacccaatc cgttatccca tgaataagat
     1741 acaccgccac ttgccatcaa cgttgtagat gcttccgcac aaattgttac atcttgtcca
     1801 gcatttattg caggcaatgg att
//
LOCUS       DAOQVQ010000492         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_84220_length_1822_cov_2.874363, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000492 DAOQVQ010000000
VERSION     DAOQVQ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84220_length_1822_cov_2.874363"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKN22_11250"
     CDS             <1..666
                     /locus_tag="PKN22_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNS43325.1"
                     /translation="DFYTMKYKGKYLAVDDNDYLIDGKKMSDATKKEIQAIGLQFDHA
                     DAKNGVSGIKDYGTSFVLLYNGEPLAYLNNELTLPDGKVLIGQDLFRLRKWAEHIRDG
                     RGDFFAKGEYGKDFVVYYKSKALDYKNRTFFLGDQKLSPYLLNKAMDSADNASSYIYV
                     ITFLHLLHIIATLLFMIRTVSYSFLGKYTNGDVIGLKATGIFWHFLDILWVFLLLFLL
                     FIH"
     gene            692..1729
                     /locus_tag="PKN22_11255"
     CDS             692..1729
                     /locus_tag="PKN22_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNS43326.1"
                     /translation="MTENATGHVNYAEAWSGKESPFKVSYGKIMMWIFLVSDALTFGG
                     FLAAYGFSREAYNGEWPIGERTFHSIPFFEGNFPLLYVAFMTFLLIISSVTMVLAVEA
                     GHRMDRKGVTKWLAATIVGGMIFVGSQAWEWSHFIHGSDFGKVELADGSKAVVHAEKF
                     GELQNFTVQTAGHKYKAGQVLTDKDLNLKHEFQHAVEAGHVHAGMITLEDGSVAKIAK
                     NEDVHMALSVVEAGGKYKLGDKIEGADASKQYYEAAYSGEAKRIVYGANLVRNEYGPT
                     VYANYFFFITGFHGFHVTIGIIINLVIFINVLKGTYEKRGHYEMVEKGGLYWHFVDLV
                     WVFVFTFFYLV"
BASE COUNT          594 a          263 c          377 g          588 t
ORIGIN      
        1 gatttttaca cgatgaaata caaaggaaaa taccttgcag ttgatgacaa cgactatttg
       61 attgatggta aaaaaatgag cgatgcaacc aaaaaagaaa ttcaagcaat tggtttgcaa
      121 tttgaccatg cagatgctaa aaatggagta agtggaatta aagattatgg aacttcattt
      181 gtgttgttat acaacggaga accattagca tatttgaaca atgaattgac attgcctgat
      241 ggtaaagtgt taattggtca ggatttgttt cgtttaagaa aatgggcaga acacatccgt
      301 gatgggcgtg gagacttttt tgctaaaggt gaatacggta aagactttgt ggtgtattat
      361 aaaagtaaag cattggacta caagaaccgc acttttttct taggtgatca aaaattatct
      421 ccatatttgt tgaacaaagc gatggattcg gctgacaatg catcttccta catttatgtt
      481 atcacatttt tacacttgct gcacatcatt gcaactttat tgtttatgat tagaactgta
      541 tcctattcat ttttaggtaa atacaccaac ggtgatgtga taggattaaa agcaactgga
      601 attttttggc actttttaga cattttatgg gtctttttat tgctattttt actttttatt
      661 cattaaactt gcaaaataaa tttaaaataa aatgacagag aacgctactg ggcatgttaa
      721 ctatgccgaa gcctggagtg gtaaagaatc tccattcaag gtgagttatg ggaaaatcat
      781 gatgtggatt ttccttgtat cagacgcatt aacttttggt ggttttttgg ctgcctatgg
      841 tttttcaaga gaagcataca atggtgaatg gccaattgga gagcgcactt tccactcaat
      901 tccatttttt gaaggtaact tcccgttact ttatgtagca ttcatgactt tcttgttaat
      961 catttcttct gtaacaatgg tattggctgt agaggctggt caccgtatgg acagaaaagg
     1021 tgtaacaaaa tggttggctg caactatcgt tggagggatg attttcgtag gatcacaagc
     1081 ttgggaatgg tcgcacttta ttcacggttc tgattttggt aaagtagaat tagcagacgg
     1141 ttcaaaagcg gtagttcatg ctgaaaaatt tggagaactt caaaacttta cagttcaaac
     1201 agctggtcac aaatacaaag ctggtcaagt acttacagat aaagatttga acttgaaaca
     1261 tgagtttcaa catgcagtag aggctgggca tgttcatgca ggaatgatta ctttagaaga
     1321 tggttctgtt gctaaaattg ctaaaaacga agatgttcac atggcattat ctgtagttga
     1381 agcaggagga aaatataaat taggcgacaa aattgaagga gctgatgcat caaaacaata
     1441 ttacgaagca gcctattctg gagaagcaaa acgtattgtt tatggagcta atttagttag
     1501 aaatgaatac ggtccaactg tatacgctaa ctacttcttc tttatcacag gtttccacgg
     1561 tttccacgta acaattggta tcattatcaa cttggttatt ttcatcaatg tattgaaagg
     1621 tacgtatgaa aaaagaggtc actatgaaat ggtagaaaaa ggtggtttgt actggcactt
     1681 tgtagactta gtttgggtgt ttgtatttac attcttctac ttagtttaat tgataaacat
     1741 taaaatttaa aataaaatgg aaagagacga tattattgag tattcattac atcatcacca
     1801 ttcagaagaa gaaggtaaaa aa
//
LOCUS       DAOQVQ010000493         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_84335_length_1821_cov_1.989241, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000493 DAOQVQ010000000
VERSION     DAOQVQ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84335_length_1821_cov_1.989241"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKN22_11260"
     CDS             <1..537
                     /locus_tag="PKN22_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43327.1"
                     /translation="KDESNSIINILHYHSEEINKPQEFEEIPEFIVDIVNSFFPLKDA
                     ITKNDFHLLYQNETVRWLIIKILSSKLISFIFEDKPIDGLKFLLGFEDGEMEQLNEFF
                     ENIDKETLIDFLTISEEDSNQFLNNKEFYNLIMGLFIKIEEKVPFEIKRFFVKLNNGE
                     INLKSKLDEIKLQITTSC"
     gene            979..1305
                     /locus_tag="PKN22_11265"
     CDS             979..1305
                     /locus_tag="PKN22_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS43328.1"
                     /translation="MKETFGEYIHNLRVEHGLTLTKLAAALDIDQSTLSKIENQKRNV
                     PEEILPKLAKVFKLDIQTLEKEFFSEKIAEMIYRVPDSTELLTLAEEKAKYYRVSKVK
                     QGNLKF"
     gene            1309..>1821
                     /locus_tag="PKN22_11270"
     CDS             1309..>1821
                     /locus_tag="PKN22_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43329.1"
                     /translation="MAKSIEQYLNKVIQGDCLEVMKNIPSKSIDMILCDLPYGTTQNK
                     WDSVIDLDQLWEHYERIIKDDGAIALTAQGLFTAKLIMSNEKLFKYKIVWIKSKSTNF
                     LNAKKQPLRKHEDICIFYKKQPTYNPQMTNGEAYDKGIRKDQYTGSYGDFKPRHVKSN
                     GERYPNDVVCY"
BASE COUNT          712 a          322 c          288 g          499 t
ORIGIN      
        1 aaagatgaaa gtaattcaat aataaacata cttcactatc actccgagga aataaataaa
       61 cctcaagaat tcgaagaaat acctgaattt atcgtagata ttgtaaattc attttttcca
      121 ttaaaggatg ccatcactaa aaatgatttt catcttctat atcaaaacga aactgttaga
      181 tggcttatca taaagatact aagctctaaa ttaatttcat ttatttttga agacaaacct
      241 atagacgggt taaagttttt actcggtttc gaagatggag aaatggaaca actaaatgaa
      301 ttttttgaaa acattgataa agaaacttta atagactttt tgacaatatc agaagaagat
      361 agcaaccaat ttttaaacaa caaagaattc tataatctga tcatgggatt gttcattaaa
      421 attgaagaaa aggttccttt tgaaatcaaa cggttttttg taaaattaaa taatggtgaa
      481 atcaatttaa aaagcaagct agatgaaatt aagttgcaaa taaccaccag ttgctaacag
      541 cacctatatg tcatgcgggg cagcggttct agcataacgc gcattacttt gaatttaaat
      601 cgtgggaact ctgaaaaagc aattcattga atccccgcac aacacatagc tgcaaccgtt
      661 accagcaagc gtagagaacg acacgaccac caaaaaacga ccttaactca atagacaatt
      721 tcgctgttct ttgacacaaa ccgtcctgac tttaccgaca ctcaagccga cccaaatggt
      781 tttaaaaatt ttttcccacc gcacttttta aaaaataatt ttatgcctac ccacattttg
      841 cacatttggt tttgccctga cacacaatgc catcccttca gcaaaaccaa aagagcaaaa
      901 tttttccaac gcacttgttc agattatgaa aataaattgt aattttgaca aaattgtcaa
      961 ggcgaaaatt tcaaggtaat gaaagaaaca tttggagaat acattcacaa tttacgagtt
     1021 gagcacggct taactctaac aaaacttgct gctgcacttg atattgacca atcaacgctt
     1081 tccaaaattg aaaatcaaaa acgcaatgtt ccagaagaaa tacttcccaa acttgccaaa
     1141 gttttcaaac tggacattca gacacttgaa aaggaatttt tcagcgaaaa aatcgctgaa
     1201 atgatttatc gagttcccga ctcaacagaa ttgttgacac ttgcagagga aaaagcaaaa
     1261 tactacagag tatcaaaggt taaacaagga aatttaaaat tttaaaaaat ggcgaaatca
     1321 atagaacaat acttaaacaa ggttattcaa ggcgactgct tagaggttat gaaaaatatt
     1381 ccaagcaaat ccattgatat gattctttgt gatttaccat acggaacaac tcaaaacaaa
     1441 tgggattcgg taattgactt agaccaactt tgggaacatt atgaaagaat catcaaagat
     1501 gatggagcaa ttgcattgac tgctcaggga ctatttacag caaagttgat tatgagtaat
     1561 gagaagttat tcaaatacaa aatcgtttgg ataaaatcta aatcaacaaa cttcttaaat
     1621 gcaaagaagc aaccgcttcg taaacacgaa gacatttgta tcttctacaa aaaacagcct
     1681 acatataatc cacaaatgac aaatggagag gcatacgaca aaggcattag aaaagaccaa
     1741 tacactggta gttatggaga tttcaaaccg agacacgtta aaagtaatgg agagagatac
     1801 ccaaatgatg ttgtatgtta t
//
LOCUS       DAOQVQ010000494         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_85931_length_1800_cov_2.831519, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000494 DAOQVQ010000000
VERSION     DAOQVQ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85931_length_1800_cov_2.831519"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKN22_11275"
     CDS             <1..338
                     /locus_tag="PKN22_11275"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNS43330.1"
                     /translation="ASDPNNSVMKLPEITTDDVELLEKWIDKLFEEFEPLRMFVLPGG
                     HPVVSHCHIARCVCRRAERVCVDLNQTEEVEPIIIKYLNRLSDYIYTLSRKQAKDLNA
                     IEKPWVARK"
     gene            360..800
                     /locus_tag="PKN22_11280"
     CDS             360..800
                     /locus_tag="PKN22_11280"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNS43331.1"
                     /translation="MKLLKTFLGFLLIVIHFGFSFSTKDVIDITITNVRNNNGKIQLQ
                     VYRDQATFSKETPYKIYYVSKTDMKNNTLHYKISDLPEGTYGIAILDDENNNKEMDWS
                     FIMMPKEGFGFSNYYHTPLSKPKFEKFKFYLNGTVNVSIKVRYM"
     gene            919..1140
                     /locus_tag="PKN22_11285"
     CDS             919..1140
                     /locus_tag="PKN22_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNS43332.1"
                     /translation="MYWTLELASYLEDAPWPAAKDELIDFAIRTGAPLEVVENLQDLE
                     DEGEIYESIEEIWPDYPSKDDFLFNEDEY"
     gene            1176..1757
                     /locus_tag="PKN22_11290"
     CDS             1176..1757
                     /locus_tag="PKN22_11290"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNS43333.1"
                     /translation="MNYLGHLYFSNNDLDLMHANLLGDFVKGKDLSAFSPTQQKGIIL
                     HRKIDDFMAKHSAVKVISKTLSPSLPKVAPIALDVYFDHFLALHWMRYHTQQLDDFLA
                     AFYAHPLQSNEYTNQQFQTLIQNMKTHQWMNQYANLYAVDRACSFLATKLSFANQLGQ
                     GRTVLEQNYTEVEQAFFAYMLDAKVYFEQEGWE"
BASE COUNT          627 a          309 c          308 g          556 t
ORIGIN      
        1 tagcatcaga cccaaataac tcggtgatga aacttccaga aattacaaca gatgacgtgg
       61 aattgttaga aaaatggatt gataaactat ttgaagaatt tgaacctttg cgcatgtttg
      121 tgttacctgg cggacatcca gttgtttctc attgccacat tgctcgttgc gtatgccgca
      181 gagccgaacg tgtttgtgtg gatttaaacc aaacagaaga agtagaaccc atcattatca
      241 aatatttaaa ccgtttgagc gattacattt atactttgag cagaaaacaa gcaaaagatt
      301 tgaatgcaat cgaaaaaccg tgggtagcaa gaaagtaatg ttttttttaa cgcaatagta
      361 tgaaactact taaaacattt ttaggatttc ttttaattgt aatccatttt ggattttcat
      421 tttccacaaa agatgtaatt gatattacca tcaccaatgt gcgtaacaat aacgggaaaa
      481 tacaactgca ggtgtatcgc gatcaagcaa ctttttctaa ggaaacaccc tacaaaatat
      541 attacgtctc aaaaacagat atgaaaaaca ataccctcca ctataaaatt agtgatttac
      601 cagagggaac gtatggtatt gctattttgg acgatgaaaa caataacaaa gaaatggact
      661 ggtcgtttat catgatgcca aaagaaggtt ttggtttttc aaattattac catactcctt
      721 tgagcaagcc aaaatttgaa aagtttaagt tttatttaaa cggcacagtt aacgtctcaa
      781 ttaaagtaag atacatgtaa attgaactta tcaacagttt aatcaaagtg ttaataaaac
      841 tttcttgtcg ttttcttgca agtaattaaa aaaaatctat ttttgccact ttaataagcc
      901 aaaaattaat tataagatat gtactggact ttagaattag catcgtattt agaagatgca
      961 ccatggcctg ccgctaagga tgaattaatt gattttgcaa tccgcacagg agcacctttg
     1021 gaagttgtag aaaacttgca agatttagaa gatgaaggcg aaatttacga aagtatcgaa
     1081 gaaatttggc ctgattatcc ttcaaaagac gattttctat ttaacgaaga cgaatattaa
     1141 atgcaatccc tccacaagag ggatttttta tagccatgaa ttacctcgga cacctctatt
     1201 tttccaataa tgatttggac cttatgcatg ccaatttatt gggcgatttt gtaaaaggta
     1261 aagatttgtc tgctttttca cctacccaac aaaaaggtat catcttgcac cgaaaaattg
     1321 acgactttat ggcaaaacat tcagcagtga aagtaatttc taaaacgtta tcaccctcat
     1381 tgcctaaagt tgctcccatt gctctagatg tttattttga tcatttttta gccttgcact
     1441 ggatgcgtta tcacacccaa caattggatg attttttagc tgcgttttat gcacatccac
     1501 tccaatcaaa tgaatatacc aaccaacaat ttcaaacctt gattcaaaac atgaaaaccc
     1561 accaatggat gaaccaatat gccaatttat atgctgtgga tagagcctgt tcttttttag
     1621 ccacaaaatt gtcgtttgca aatcaattgg ggcaaggacg cactgtgttg gagcaaaatt
     1681 atacagaagt agaacaagca ttttttgcct atatgcttga tgcaaaagta tattttgaac
     1741 aagaagggtg ggagtagata aaaggtaaaa aaaacataca tctcaattct gattaatttg
//
LOCUS       DAOQVQ010000495         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_86216_length_1796_cov_3.139001, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000495 DAOQVQ010000000
VERSION     DAOQVQ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86216_length_1796_cov_3.139001"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..972
                     /locus_tag="PKN22_11295"
     CDS             46..972
                     /locus_tag="PKN22_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNS43334.1"
                     /translation="MELLKQLTAIRGASSDEGNIAQFILDYVAQNTPNWKVQPTVLSG
                     GDFHDCVVLIFGKPTTAIYAHIDTIGYSVGYENNLIKIGGPTIEDGIQLVGCDSQGEI
                     ATELMVIENDDDTISLTCVFDRTIDRGTILTYKPNFIESDVYVQSPYLDNRLGVWSAL
                     KVAEQLENGAIVFSTYEEHGGNSVGFCANYLYQNYNIRQALISDITWVTPGVLHGKGV
                     AISLRDKYLPRKSFLNKIVALAEQSGVMFQLEVESAGGSDGSLLQLSHLPIDWCFIGA
                     PEDHVHTPNELVYKSDIMNMVLLYQYLMEKLD"
     gene            1068..1274
                     /locus_tag="PKN22_11300"
     CDS             1068..1274
                     /locus_tag="PKN22_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43335.1"
                     /translation="MKKVLFAGLAILALASCKKDWTCTCQYEGNASSYQIVNRTKKDA
                     KQICTGSVSVGLITVAGDKNCSLN"
     gene            1352..1534
                     /locus_tag="PKN22_11305"
     CDS             1352..1534
                     /locus_tag="PKN22_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNS43336.1"
                     /translation="MKKVVFAALAVLALASCKKEYTCTINGVSSATYSEKNYTSAQMD
                     ALKTTCELAGGTWKAK"
BASE COUNT          612 a          268 c          337 g          579 t
ORIGIN      
        1 ctaaattagg tgaaataaaa attatttttc tgggaaaata ttaaaatgga attattaaaa
       61 caattaacag caataagagg tgcatcaagc gatgaaggga atattgctca atttatactt
      121 gattacgttg ctcaaaatac acctaattgg aaagtgcaac caaccgtttt gtcgggtgga
      181 gattttcacg attgtgtggt gctaattttt ggcaaaccta caactgcaat ttatgcacac
      241 attgacacca ttggctattc tgttggttac gagaataatt taattaaaat tggcggtcct
      301 accattgaag atggcattca actggttggt tgcgactctc aaggtgaaat tgcaaccgaa
      361 ttgatggtta ttgaaaacga cgacgatacc atttcgctca cgtgtgtgtt tgaccgtacg
      421 atagaccgag gaacgattct tacctacaaa cccaatttta tagaaagcga tgtgtatgtg
      481 caatcgcctt atttggacaa ccgtttgggt gtttggtcgg cattaaaagt tgctgaacaa
      541 ctagaaaatg gcgctatagt gttttccacc tacgaagagc atggaggcaa cagcgttggg
      601 ttttgcgcca actatttgta tcaaaactac aacattcgcc aagcattgat ttccgatatc
      661 acatgggtaa cgccaggtgt tttgcatgga aaaggtgttg ccatttcatt gcgcgataaa
      721 tacttgcctc gtaagtcgtt tttaaataaa attgttgcat tagccgaaca atcgggtgta
      781 atgtttcaat tagaagtaga atcggcaggc ggaagcgatg gaagtctctt gcaattatcg
      841 caccttccaa ttgactggtg ttttattgga gctcccgaag atcatgtgca tactcccaac
      901 gaattggtgt ataaaagcga catcatgaac atggttttat tgtaccaata cttgatggaa
      961 aaactggatt aaactacgta aaacttcgta tttcagcgat aatagtttgc tatttgcaaa
     1021 ttttaacata tatttgtttt atattattaa cacataaatc tataattatg aaaaaagttt
     1081 tattcgctgg tttggctatt ttggctttgg cttcttgtaa aaaagactgg acatgtacat
     1141 gtcaatacga aggaaatgca tcatcttacc aaattgttaa cagaacgaag aaagatgcta
     1201 aacaaatttg tacaggatct gtatcagtag ggttaatcac agtagctgga gataaaaatt
     1261 gttctttgaa ctaatatttc aattattttt taaattctat ttcattttat tacatatatt
     1321 tgttttaaaa ttaacattaa aattaaacat tatgaaaaaa gtagtatttg ctgcattagc
     1381 agttttagct ttggcttctt gcaaaaaaga gtacacatgt acaattaatg gtgtttctag
     1441 tgcaacatat agtgagaaaa attacactag cgcacaaatg gacgcattaa aaacaacatg
     1501 tgaattagct ggaggtactt ggaaagctaa atagtttttt acaatacaat tcaaaaaagg
     1561 aatctgaaaa ggttcctttt tttgtttttc aattttcata ttgtaaaaaa acgattaaat
     1621 ttgagaacat aaaattaata gtgaattatg aaaaaattat ttttagttgg aattgcattt
     1681 tcagcacttg tatcttgtaa gaaaaattat acgtgtgaat atacattaat tgacacaacc
     1741 tataaatctg agtataaaga attggataag aaacaagcta aagctgcaaa agagag
//
LOCUS       DAOQVQ010000496         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_86679_length_1790_cov_3.448415, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000496 DAOQVQ010000000
VERSION     DAOQVQ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86679_length_1790_cov_3.448415"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="efp"
                     /locus_tag="PKN22_11310"
     CDS             <1..535
                     /gene="efp"
                     /locus_tag="PKN22_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNS43337.1"
                     /translation="CIKHNDKLCQIIEFQHVKPGKGPAFVRTKMRQIETGKILDFTFS
                     AGHKIEPVRIENREYQYLYEEADAFVFMNNETYEQVLIPTAMIEKPKFLQEGLVCNIL
                     FHAEEEMPLVVELPMYIISEVTYTEPGVKGDTATNSMKPATIATGAEVKVPLFIDNGE
                     VIKIDTRTGTYVERVKG"
     gene            complement(639..1592)
                     /locus_tag="PKN22_11315"
     CDS             complement(639..1592)
                     /locus_tag="PKN22_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS43338.1"
                     /translation="MSDKAALDQLVQHYNELKNEIHKVIVGQDEVVDQVLISIFSRGH
                     CLLVGVPGLAKTLLVNTITQTLGLSFNRIQFTPDLMPSDIIGSEILDESRNFKFIKGP
                     IFNNIILADEINRTPPKTQSALLEAMQERSVTTAGKTYKLEAPFFVLATQNPIEQEGT
                     YPLPEAQLDRFIFNIWVDYPTFVEEMAIVRATTVDSKTELKQVLNAEQIIAYQELIRR
                     IPVADNVLEYAVKLVGKTRINSEFATPLTKDYITWGAGPRASQYLIIAAKCHAALNGK
                     YSPDIEDVKAVALPILRHRLVRNYRAEAEGYSMEKIIKELL"
BASE COUNT          557 a          313 c          331 g          589 t
ORIGIN      
        1 atgtattaaa cacaacgata aattgtgtca aattattgaa tttcaacacg taaaaccagg
       61 taaaggtcct gcatttgtac gtacaaaaat gcgtcaaatt gaaactggaa aaattttgga
      121 tttcactttc tcagcaggtc acaaaattga accagttcgt attgaaaaca gagaatacca
      181 atatttgtat gaagaagcag atgcatttgt tttcatgaac aacgaaacgt atgaacaagt
      241 gttgattcca actgcaatga ttgaaaaacc aaagttttta caagaaggtt tggtatgtaa
      301 cattttattc cacgctgaag aagaaatgcc attggtggtt gaattgccaa tgtacatcat
      361 ttctgaggtt acttacacag agccaggtgt gaaaggagat acagcaacaa actctatgaa
      421 acctgcaaca attgcaacag gagcagaagt aaaagttcct ttgtttattg acaacggtga
      481 agtaatcaaa attgatacac gcacaggtac atacgtagaa agagtaaaag gatagtagtt
      541 cactattgag gatagtaaaa aaggcttaga tttctctaag ccttttttgt gtttattaaa
      601 atgtaattga atttttaaga actttcagct aaaaaaaatt acaacaactc ttttataatt
      661 ttttccattg aatagccttc tgcttcggcg cggtaatttc ttaccaaacg gtgacgtaaa
      721 ataggcaatg caactgcttt tacatcttca atgtcgggag agtatttgcc attcaaggct
      781 gcgtggcatt ttgctgcaat gattaaatat tgcgaagcac gtggaccagc tccccaagta
      841 atataatctt ttgttaatgg tgttgcaaat tctgagttga tgcgtgtttt tccgactaat
      901 ttcaccgcat attccaacac attgtcggca actggtattc tgcgaatcaa ttcttggtag
      961 gcaataattt gttcggcgtt caacacttgt tttaactcgg ttttactgtc aacagttgtg
     1021 gctcttacaa ttgccatttc ttcaacaaaa gtagggtagt ccacccaaat attgaaaata
     1081 aaacggtcga gttgtgcttc tggcaacgga taagttcctt cttgctcaat agggttttgt
     1141 gttgctaaca caaaaaatgg cgcttccaat ttataggttt taccagctgt agtaaccgaa
     1201 cgttcttgca ttgcctccaa caacgccgat tgtgtttttg gtggcgtacg gttaatttcg
     1261 tcggccaaaa taatgttatt aaaaatcggg cctttgatga atttaaaatt tctggattca
     1321 tccaatattt cagaaccaat aatgtctgac ggcattaagt cgggtgtaaa ctgaatacgg
     1381 ttaaacgaca atcctaacgt ttgagtaatg gtgttgacaa gcaatgtttt tgcaagtcct
     1441 ggcacgccca caagtaaaca atgtcctcgt gaaaaaattg aaatcaatac ttgatctaca
     1501 acttcatctt gtccaacgat tactttgtga atttcgtttt ttaattcgtt ataatgttgt
     1561 actaattggt ctaaagcagc tttatctgac atggttaaga tttttgataa atgtacaaaa
     1621 aattacttca aattccagtt gtatttaaac gaacagtttt tgtattcgtc gttgattttc
     1681 acatacgcct ggctgatttt tcctttcacc caagcatcaa tcatttcttg gtgtttggtg
     1741 ctttcagctg ctttttgaat caacggataa tcttctttta aattagcttt
//
LOCUS       DAOQVQ010000497         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_87141_length_1785_cov_3.726012, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000497 DAOQVQ010000000
VERSION     DAOQVQ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87141_length_1785_cov_3.726012"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="folK"
                     /locus_tag="PKN22_11320"
     CDS             <1..409
                     /gene="folK"
                     /locus_tag="PKN22_11320"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNS43339.1"
                     /translation="EINGNDRFNTAMIAIGSNIEPTFNIEQALEMIASECTVMKKTEF
                     ITTPPLKFEAQADFVNGALLVATRLSYNELHAFLRKIEALLKRERTENKNAPRTIDLD
                     ITTFNGVLVDKEIHELPFLVDFLTILQPELSNN"
     gene            500..>1785
                     /locus_tag="PKN22_11325"
     CDS             500..>1785
                     /locus_tag="PKN22_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNS43340.1"
                     /translation="MTAKEKVAQLRAAMQEQNIEAFIVYSADPHMSEYLPEEWKERTW
                     LSGFTGSAGFVVVTKNNAGLWTDGRYFTQAPIELAGSGIELFKDGMEGTPNYIDWIIS
                     ETQEGSAVAVNALCTSNANWEMLHTKLAAANRQLKDLPLLKNIWTNRGVAEKNPIFVQ
                     PIARAGKSVEDKLADIRSKMKEMNASFHLLSSLDDIAWTLNLRGSDVECNPVFLSYLT
                     ISATSATLFVDTEKLNDEARQAMQQAKVNVVEYTQFFTALTEIKNERILIATSCNQSV
                     FDALSANNSFIKAAAPGNLMKAQKNDTELNGFRSAMVKDGVAMVKFLYWLTHQAGKEA
                     MTEYTIGQKLRGFRAEQKDFVGESFDSIVGYKGNGAIIHYKAPKEGCAEVTNDGSILV
                     DSGGQYLEGTTDITRTFPLGAVSEEFKHDSTLVLKG"
BASE COUNT          591 a          314 c          376 g          504 t
ORIGIN      
        1 agaaataaat ggcaacgatc gtttcaatac tgcaatgatt gcaattggtt ccaacattga
       61 acctactttc aacattgaac aagcacttga aatgattgct tcggaatgta cggttatgaa
      121 aaaaacagaa ttcattacca ccccaccctt aaaatttgaa gcacaagccg attttgttaa
      181 cggagcacta ttggttgcaa ctcgattgag ttacaatgaa ttacatgcat ttttacgaaa
      241 aatagaagcc ttgctcaaaa gagaacgcac tgaaaataaa aatgctcctc gcactattga
      301 tttagatatt accacattca acggtgtgtt ggtggataaa gaaatccacg aattgccttt
      361 tttggttgat tttctaacca ttttacaacc agaattgagc aataattagt ctattttatc
      421 catttcttgg gctaaaaaaa tgctttttgt tttttatttt gtttggtaac ttgtactttt
      481 acattcaaac acaaataaaa tgacagcaaa agaaaaagtg gcgcaattac gtgctgccat
      541 gcaagaacaa aatattgaag catttatcgt ttattcggct gatccacaca tgagtgaata
      601 cttacccgaa gaatggaaag aaagaacgtg gctatccgga tttacaggtt cggctggttt
      661 cgttgtggtt actaaaaaca acgcaggttt atggaccgat ggccgttatt ttacgcaagc
      721 accaattgaa ttagcaggtt ctggcattga attgtttaaa gatggaatgg agggaacacc
      781 caactacatc gattggatta tttctgaaac gcaagaagga agcgccgttg ccgttaatgc
      841 tctttgtact tctaatgcca attgggaaat gttgcacact aaattagctg ctgccaacag
      901 acaactaaaa gatttgccgt tgctaaaaaa tatttggacc aacagaggtg tagcagaaaa
      961 aaatccaatt tttgttcaac ccattgcacg agctgggaaa tcggttgagg ataaattagc
     1021 cgacattcgt tctaaaatga aagaaatgaa cgcaagtttt cacctgcttt cgagtttgga
     1081 cgatattgct tggactttaa acctacgtgg tagtgatgtt gaatgcaatc ctgtgttttt
     1141 gagttattta actattagcg caacaagtgc tacgttgttt gtggatacag aaaaattgaa
     1201 cgatgaagct cgccaagcaa tgcaacaagc aaaagtgaat gtggtagaat atacacaatt
     1261 ttttacggca ttaacagaaa taaaaaacga acgcatttta attgcaacca gctgtaatca
     1321 atcggttttt gatgctttat ctgccaacaa ttcgtttatc aaagcggctg ctccgggcaa
     1381 tttgatgaag gcgcaaaaaa acgataccga gttaaacgga ttccgttcgg caatggtgaa
     1441 agacggtgtg gcaatggtga aatttcttta ttggctaacc catcaagcgg gcaaagaagc
     1501 aatgacagaa tataccattg gtcagaaatt gagaggattt agagccgaac aaaaagattt
     1561 tgtaggcgaa agttttgata gcattgttgg ctacaaaggc aacggggcaa ttattcacta
     1621 caaagcacca aaagaaggat gcgccgaagt taccaacgat ggtagtattt tggttgattc
     1681 gggaggtcag tatttggaag gaacaaccga tattactcgc acatttccac tgggggcagt
     1741 atccgaagaa tttaagcacg attctacgct ggtgttgaaa ggaca
//
LOCUS       DAOQVQ010000498         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_87250_length_1784_cov_2.689416, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000498 DAOQVQ010000000
VERSION     DAOQVQ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87250_length_1784_cov_2.689416"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1548
                     /gene="rocD"
                     /locus_tag="PKN22_11330"
     CDS             301..1548
                     /gene="rocD"
                     /locus_tag="PKN22_11330"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNS43341.1"
                     /translation="MSIVADGTRSSELIALEDKYGAHNYHPLPVVLTKGEGVYVWDVE
                     GKRYYDFLSAYSAVNQGHSHPKIVKAMIEQVQQLALTSRAFYNDVLGVYEKTITEYFG
                     FDKVLPMNSGAEAVETAIKLCRRWAYEKKGIAENDATIIVCDGNFHGRTTTIISFSND
                     PDSRINYGPYTPGFVSIPYGDVDALAEVLKNNKNIAGFLVEPIQGEAGVMVPQEGFLA
                     IAKALCEENNVLFIADEIQTGIARTGKLLAVNYENVTPDILILGKALSGGLYPVSAVL
                     ANDEIMMVIKPGQHGSTFGGNPIAARVAIAALEVVKEEKLAENAYRLGEIFRKEMNRI
                     IEKSDLITKVRGKGLLNAIIVNDTPESSTAWDLCMQLAENGLLAKPTHGNIIRFAPPL
                     VMTEEQLMECVAIIEKTITEFKK"
BASE COUNT          590 a          271 c          379 g          544 t
ORIGIN      
        1 tagagcagtg gatgtgtcca atgatttgta tttaactggt tatgctaatt attgggaaat
       61 tattaatgca caaaaagcta aaattcaagc cgaattggat tatgtagatt ttcaacaccg
      121 taatgcaaaa gcgttggtgc aattgtacaa agccttgggt ggagagatta aatgatttta
      181 atatttaacg tgtgaaattt caaaaaacac acttgtaaca aggttttttt aacagataat
      241 tggtattctg aattgcattc aatacatttg taattcatta aattaattaa aaaagttgtt
      301 atgagcatag ttgcagatgg cactcgttcg agtgaattga tagcgttgga agataaatat
      361 ggtgcccaca attaccatcc actaccggta gttttaacaa aaggtgaggg agtttatgtt
      421 tgggatgtgg aaggaaaaag atattacgac tttctttcgg catattcggc agtaaaccaa
      481 ggtcatagcc atccaaaaat tgtgaaggca atgattgagc aagtgcaaca attggcatta
      541 acatctcgtg cattctacaa cgatgtattg ggtgtgtatg aaaaaacaat taccgaatat
      601 tttggttttg ataaggtgtt gccaatgaac tcaggtgctg aggcggttga aacagctatt
      661 aagttgtgtc gccgttgggc ttatgagaaa aaaggcattg ccgaaaacga tgcaacaata
      721 attgtgtgcg atggtaactt ccacggaaga acaacaacca tcatttcgtt ttccaatgat
      781 ccagattcac gcattaacta cggaccctac acaccagggt ttgtttccat tccttatggc
      841 gatgttgatg ccttggcaga agtattgaaa aataacaaaa acattgctgg atttttagta
      901 gaaccaattc aaggtgaagc aggggtaatg gtgcctcaag aaggattttt ggcaattgca
      961 aaagctttgt gcgaagaaaa taacgtcttg ttcattgcgg atgaaattca aacaggaatt
     1021 gctcgtactg gaaaattatt ggctgtaaat tatgaaaatg taacacccga tattttgatt
     1081 cttggaaaag cactttctgg tggtttgtat ccggtttctg ctgttttggc aaacgacgag
     1141 ataatgatgg tgattaaacc agggcaacac ggttctactt ttggaggtaa tccaattgcg
     1201 gcacgtgtag ccattgctgc tttagaagtt gttaaagaag aaaaattggc agaaaatgct
     1261 taccgtttag gtgaaatttt tagaaaagaa atgaaccgca tcatcgaaaa atcggatttg
     1321 ataacaaagg tgagaggtaa aggtttgttg aacgccatta ttgtaaacga cacaccagaa
     1381 agctcaactg cttgggattt gtgtatgcaa cttgcagaaa acggattgct agcaaaacca
     1441 acacatggta acatcattcg ctttgcgcca cctttggtga tgacagaaga acaattgatg
     1501 gaatgtgttg ctatcatcga aaaaacaatt acagaattta aaaaataaaa tattcttaca
     1561 catctgaaat ggttgttcga aatgttttta attttggaca accattttat agcatgttta
     1621 accttttagc aaccttcaat aattttttat atttgctaga actaatttta tattaatttg
     1681 acaacaaaaa ttaaattcgg aacagacggt tggagagcaa ttattgccga tgaatatacc
     1741 gttgataatg ttgcaagagt ttctttggca actgccattt gggt
//
LOCUS       DAOQVQ010000499         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_87732_length_1778_cov_4.134068, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000499 DAOQVQ010000000
VERSION     DAOQVQ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87732_length_1778_cov_4.134068"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKN22_11335"
     CDS             <1..274
                     /locus_tag="PKN22_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNS43342.1"
                     /translation="EVKKQRADAIMELQSGISYELNQQKVGKTFKVLFDRVEGDYFIG
                     RTEFDSPEVDNEVLVKKSEHFVRIGDFALVEITSADHYDLYGKVVD"
     gene            277..1698
                     /locus_tag="PKN22_11340"
     CDS             277..1698
                     /locus_tag="PKN22_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS43343.1"
                     /translation="MELSPFKQQVLQQFQYDFTADQSVCVDQLELFLKDTTPYCVFLL
                     SGYAGTGKTSLLGAFIKTLAHFKLNTVLLAPTGKAAKVLSNKSAKRAFTIHKMIYRRK
                     SKTDDFSTIQLAPNLQKNTTFIVDEASMIGDYTMVDSNINQRNLLEDLLEFVYSGNNC
                     KLVLLGDVGQLPPVGATFSPALNKAYLINNFPRLTIYSANLNEVLRQQQNSSILTNAT
                     QLRNQQKGYYQFHIPPKSDMVLVEGSELQDALENSFANWGTDETILITRSNKRCNEYN
                     RQIRARVFWYEDVLCNGDLLMVVKNNYHWLKEEPEMGFIANGETMVVKRVVKYEKIYG
                     FEFARLLVTFPDYEQIGEQEVIVHTESLNCEGASLSRERLKELFFEVEKDYLYEPNKK
                     KRYELILANPYFNALQVKFAYAVTCHKSQGGQWEHVFIDVGFLPEDLDVLEYNRWLYT
                     ALTRAKSKVYLVNFPKEQLISKD"
BASE COUNT          579 a          315 c          346 g          538 t
ORIGIN      
        1 agaggtaaaa aaacaacgtg ccgatgccat aatggaattg caatctggga ttagttacga
       61 attgaaccaa caaaaagtgg gtaaaacttt caaagtgttg tttgatcgtg tggaaggcga
      121 ctatttcatt ggtagaacag agtttgattc gcccgaggtg gacaacgagg tgttggttaa
      181 aaaatcggaa cattttgtac gcatcggcga ttttgcactt gttgaaatta ccagtgcaga
      241 tcattacgat ttgtatggaa aagtggttga ctaaccatgg aactttcgcc gtttaaacaa
      301 caagttttac agcaatttca atatgatttt actgccgatc aatcggtgtg tgtggaccaa
      361 cttgagcttt ttttaaaaga caccacgcca tattgtgtgt ttttgttgag cggatatgct
      421 ggcacaggaa aaaccagttt attgggcgct tttatcaaaa cattggctca tttcaaactc
      481 aacacggttc tgttggcacc cacaggtaag gctgcaaaag ttttgagcaa caaaagtgct
      541 aaacgtgctt ttactattca taaaatgatt tatcgtagga aaagtaagac cgacgatttc
      601 tcaaccatac aattggcacc caacttgcaa aaaaatacca cttttattgt tgacgaagct
      661 tctatgattg gcgattacac aatggtagat agcaatatca atcaacgcaa tttattggaa
      721 gatttactgg aatttgtata tagtggcaac aattgtaaac ttgttttatt aggcgatgta
      781 ggacagttac ctcccgttgg cgctactttt tcaccggcat taaacaaagc gtatttgatt
      841 aacaattttc ctcgtttaac catttattcc gcaaatttga atgaagtttt gcgtcaacag
      901 caaaattcat caattttaac caacgccaca caacttagaa atcagcaaaa aggttactac
      961 caatttcaca ttcctcccaa aagcgatatg gtattggtgg aaggtagtga acttcaagat
     1021 gccctcgaaa attcgtttgc caactgggga accgatgaaa ccatcttaat caccagatcc
     1081 aacaaacgct gcaacgaata caaccgtcaa attcgcgcac gtgtattttg gtatgaagac
     1141 gttttgtgca acggagattt attgatggtt gtaaaaaaca actaccattg gctaaaagaa
     1201 gaacccgaaa tgggatttat tgcaaatggc gaaacaatgg tggtaaaacg agttgttaaa
     1261 tacgaaaaga tttatgggtt tgagtttgct cgactacttg taaccttccc cgattacgaa
     1321 caaattggcg aacaagaagt cattgttcat accgaatcgt taaactgcga aggtgcctct
     1381 ttgtcacgtg agagactcaa agaattgttt tttgaagttg aaaaagatta cctttacgaa
     1441 cccaacaaga aaaaacgcta tgaattgatt ttggcaaacc catatttcaa tgccttgcaa
     1501 gtaaaatttg catacgccgt tacctgtcat aaatcgcaag gcggacaatg ggaacacgta
     1561 tttattgatg ttggtttcct acctgaagat ttggatgtgt tggaatacaa tcgttggtta
     1621 tacaccgcat taacacgtgc taaatcaaaa gtgtatttag taaactttcc aaaagaacag
     1681 ttgatttcta aagattaaat tattcctgca atatcgaaat atttcgatat gtaaatttct
     1741 aattattatt ttgagtttta aaattttatt tttatctt
//
LOCUS       DAOQVQ010000500         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_87970_length_1775_cov_5.000000, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000500 DAOQVQ010000000
VERSION     DAOQVQ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87970_length_1775_cov_5.000000"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1254)
                     /locus_tag="PKN22_11345"
     CDS             complement(34..1254)
                     /locus_tag="PKN22_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43344.1"
                     /translation="MRYFLTFLVFSCILGVNAQKFTVYKTTQPFYDIIEWKGMGGLLL
                     SKDPSGNSNQIYLTLVGEQTKSSWEQRFAPLDGKYTYIASENARYVYFLRSLQPDAGK
                     IYFDQLNSAGNIKNTSVSYVSVIKNLGFDPYSLELVNVVVTDKALVHHFRYFDKKEKT
                     HTDIATFITHHNMLIYATVLGKIHDDELKANRSHHIQYAGFTGDEICFSGYSETKNGK
                     GWNAVVYNSKGQELKRFLIKNTLGSTTAFPTSGFGTNGAYYLNSQNTTQNGFITFHNG
                     KYYVTTLNTTTAPQLELHVLADEKLTLVNKFEIAKNALKKPLNIGVYPVNEALIYRLS
                     ASELGDKTVALFTNGSQPFLSDYSNLVVYNISRALMPTPPSTFVATLAQYNLFFDRTQ
                     LKTTTDMEFELKQK"
     gene            complement(1257..>1775)
                     /locus_tag="PKN22_11350"
     CDS             complement(1257..>1775)
                     /locus_tag="PKN22_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43345.1"
                     /translation="TRCGGGVGVGAAVGAAGAILSGVLNDGGEKIAGVTGGIVEATLG
                     TLMLVNQKKVYFEHKRNALGEVWNNVSKSEIFPPSIWYYLTSSDLNDDDQESKVHQII
                     ERWMEFEQFDEVKEKKRKKLIQLYFGEGGDYTTEQLFNRANMLDQLESHIKLMKQELT
                     LLTMEINQYRIN"
BASE COUNT          554 a          340 c          290 g          591 t
ORIGIN      
        1 aaaacaatat ctcacttttt taattgatgc aactcatttt tgttttaatt caaattccat
       61 atcagttgtt gtcttaagct gcgttctgtc aaaaaacaaa ttgtattgcg ccaaagtagc
      121 aacaaaagtt gatggtggtg taggcattaa tgcgcgacta atgttgtaaa ccaccaaatt
      181 gctataatca ctcaaaaaag gttgacttcc gttggtaaac aatgctacgg ttttatcacc
      241 aagttcacta gcagataagc gataaatcaa agcttcgttg acagggtaaa caccaatgtt
      301 gagtggtttt tttaatgcat tttttgctat ttcaaactta tttaccaacg taagtttttc
      361 atctgccaat acgtgtaatt ctaattgcgg agctgtagtt gtgttgagcg ttgtaacgta
      421 atatttgcca ttatgaaagg tgatgaaccc gttttgagtc gtattttgag aattcaaata
      481 ataagctcca tttgtaccaa atccactagt tggaaaagca gttgttgaac cgagtgtgtt
      541 ttttattaaa aatcgtttca attcttgccc tttggagttg taaacaacag cattccatcc
      601 cttgccattt ttggtttcag aataacccga aaaacaaatt tcatcccctg taaagcctgc
      661 gtattgaatg tgatgagaac gattggcttt taattcatca tcgtggattt tgcctaaaac
      721 ggttgcataa atcaacatgt tgtgatgggt aataaacgta gcgatatcgg tgtgcgtttt
      781 ttctttttta tcaaaataac ggaaatgatg caccaaagct ttatcggtta caactacgtt
      841 aaccaattca agcgaataag ggtcaaaacc taagttttta attacagaaa cataagaaac
      901 gcttgtgttt ttgatgtttc cggcagagtt taattggtca aaataaattt ttccagcatc
      961 gggttgcaaa ctacgtaaaa aatatacata acgcgcattt tcactagcga tgtaggtgta
     1021 ttttccatct aatggcgcaa aacgttgttc ccaactactt ttagtttgtt caccaaccaa
     1081 agtaagatat atttgattgc tatttccact aggatctttg cttagtaaaa gtccgcccat
     1141 acctttccat tcgatgatgt cataaaatgg ttgcgtggtt ttataaactg taaatttttg
     1201 agcattgaca cccaaaatac aactaaacac taaaaaagtt aaaaagtatc tcataattaa
     1261 ttgattctgt attggttaat ctccatggtt aaaagggtca attcttgttt catcaattta
     1321 atgtgtgatt ccaattgatc taacatatta gcacgattaa acaattgttc tgtggtgtaa
     1381 tcacctcctt cgccaaaata aagttgtatt aattttttac gttttttttc ttttacttca
     1441 tcaaattgct caaattccat ccatcgttca ataatttgat gcactttaga ttcttggtca
     1501 tcgtcattta agtcactaga agtaagataa taccaaatgg aaggaggaaa aatttcggat
     1561 tttgaaacat tattccatac ttctcccaag gcattgcgct tatgttcaaa ataaaccttt
     1621 ttttgattca ccaacatgag cgtacccaaa gttgcctcca caataccacc agttactcca
     1681 gcaatttttt caccaccatc attgagcaca ccagataaga ttgcacccgc cgcaccaaca
     1741 gcagcgccca caccaacacc accgccacaa cgcgt
//
LOCUS       DAOQVQ010000501         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_88579_length_1768_cov_2.392294, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000501 DAOQVQ010000000
VERSION     DAOQVQ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88579_length_1768_cov_2.392294"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKN22_11355"
     CDS             <1..401
                     /locus_tag="PKN22_11355"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43346.1"
                     /translation="PAFQAMLTTALAVVSEPDEKIMLVTGLPLVSYKQSREDFKKFLL
                     DFTAEVEIEGDIKNIKVKDVYVFPQAAGIFLNPACEEFKQDMATIKALQQETGITPEK
                     LMDNQEYLIKMGGLLPKQQRNEVESSFSRM"
     gene            419..1267
                     /locus_tag="PKN22_11360"
     CDS             419..1267
                     /locus_tag="PKN22_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS43347.1"
                     /translation="MNATQKQQIANEVIRLSSHSSQGMIAHRAGVSTATISQVVNNNW
                     KLIAPEMWRKIAVSLHMDTEWNHGDTANDKILNDLLLAAHTRSLAIGISHEAGAGKSH
                     AYKRYMKSYKNVIYVECKNFWSKKTFVKNLLSASGIKSNGTAEEMIEAFLDHVMKLEK
                     PLIIFDQIDKLKDTQMDLFMDFYNDLPHCGFVCSGVPALKKRILRGVQRDKIGYNEFY
                     SRIGRKFIQLDPISLQDVTNVCNANGITDEEFINYTFNTCDGDFRRVRRSIEQYFLMN
                     KKKQAS"
     gene            1264..>1768
                     /locus_tag="PKN22_11365"
     CDS             1264..>1768
                     /locus_tag="PKN22_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43348.1"
                     /translation="MKLKRAISYHELASKKFKTMDLEGAFLDLIGKPERTGAWIIYGE
                     SGNGKTRFSLQLAKELAKFSRIGYNSIEEGAKLTFQRAIMETNLKPIARRFTIIPGDN
                     LIELKQRLNRDKSPNIIFIDSIQYLTDGSEQHRNITHLEYKELISRFPTKLFIFISHA
                     DKGEPRGA"
BASE COUNT          632 a          357 c          336 g          443 t
ORIGIN      
        1 accctgcgtt tcaggcaatg cttaccacag cattagctgt ggtaagtgaa ccggacgaaa
       61 aaattatgct ggtaactggt ctaccattag tatcttataa acaatcccgg gaggatttta
      121 aaaaattttt actggatttt actgccgaag ttgaaattga gggggacatt aaaaatataa
      181 aagttaaaga tgtatatgtg ttccctcaag cagctggtat atttttaaac cctgcatgtg
      241 aagaatttaa acaagacatg gcaacaatca aagcattgca gcaagaaaca ggtatcacac
      301 cagagaaatt aatggacaat caggaatatt taataaaaat gggcggtctg ctcccaaaac
      361 aacaacgaaa cgaagttgaa agcagttttt caagaatgta aataaaaccc tctcaattat
      421 gaatgcaaca caaaaacaac aaatcgctaa cgaagttatc agactaagca gtcattcatc
      481 acaaggaatg atagcgcaca gagctggtgt cagtacagca accatcagtc aagtagtcaa
      541 taacaattgg aagcttatcg ctccggaaat gtggcgaaaa atcgcagtat ccttacacat
      601 ggatacagaa tggaaccacg gtgatacagc taacgacaaa atattaaacg atctattatt
      661 ggcagcacac actcgtagct tggcaatcgg tatttcgcac gaagctggtg caggtaaatc
      721 acacgcatac aaacgttaca tgaagtctta caaaaatgtg atttatgtgg agtgcaaaaa
      781 cttctggagc aaaaaaacat tcgtaaaaaa cttgctttct gcttcaggca tcaaatcaaa
      841 cggcacagct gaagaaatga tagaagcatt tttggaccac gttatgaaac tcgaaaaacc
      901 actcatcatc ttcgaccaaa ttgacaagtt gaaagataca caaatggact tgtttatgga
      961 tttttacaac gacttaccgc actgtgggtt tgtttgttcc ggtgttcctg ccttgaaaaa
     1021 aagaatccta cgtggtgtgc aacgtgataa gattggctac aacgagtttt actcacgcat
     1081 tggcagaaag ttcattcaac tagatcctat atccctacaa gatgttacca atgtatgcaa
     1141 cgccaatgga ataacagatg aggagtttat caactataca ttcaatacct gcgatggcga
     1201 tttcagacgt gtacgcagat ccatagagca atatttccta atgaacaaaa agaaacaagc
     1261 atcatgaaac taaaacgagc aatatcttac cacgaactag cgagtaagaa gttcaaaaca
     1321 atggacttgg aaggtgcgtt cttggatttg attggcaaac cagaacgcac aggagcttgg
     1381 attatctacg gtgaatcagg taacgggaaa acacgctttt cgttgcaact ggcaaaagaa
     1441 ctggctaagt tttcacgcat tggctacaac tccatcgaag aaggtgctaa gctaactttt
     1501 caacgtgcca tcatggaaac caaccttaaa ccaatagcac gcagatttac aattatccct
     1561 ggtgataatt taatagagtt aaaacagcgt ttaaatcgtg ataaatcacc caacatcatt
     1621 ttcattgatt ctattcaata cctaacagat ggttcagaac aacatcgcaa catcacgcac
     1681 ttagaataca aagaattgat ttcacgcttt ccaaccaaac tcttcatctt catttcacat
     1741 gccgacaaag gcgaaccacg tggagcag
//
LOCUS       DAOQVQ010000502         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_88810_length_1765_cov_2.682456, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000502 DAOQVQ010000000
VERSION     DAOQVQ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88810_length_1765_cov_2.682456"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="rsgA"
                     /locus_tag="PKN22_11370"
     CDS             complement(<1..537)
                     /gene="rsgA"
                     /locus_tag="PKN22_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HNS43349.1"
                     /translation="MKGKVLKSTGKWYIVELSDGSIVNCRIRGKIRLEGLSTTNPIAV
                     GDNVVLADDIDDEGNRVITGFEKRSNYIVRKSTNLSKQMQILAANVDRAYLVITIKNP
                     TTQLAFIDRFLVAAESFRIPTTLLINKIDLYTPDDLEYAQALAHMYEQIGYPTHLISA
                     ENEQSIAFLRDEIVGKQVM"
     gene            complement(554..1441)
                     /locus_tag="PKN22_11375"
     CDS             complement(554..1441)
                     /locus_tag="PKN22_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNS43350.1"
                     /translation="MKFGAIDIGTNAARLLIGEVIKDDGKKFINKVSYTRIPLRLGES
                     VFNDGTINSKKAEQFVKTMKAFQLIAELFEVKELRACATSAMRDASNNSVVKEQILNA
                     TGIEVETISGDEEARLISGTFKLMKLEKTNPYIVVDVGGGSTEISVFINGKKERAQSF
                     EIGTIRVLFGKTNATIWDEIYQWLEKNVANINGEITFYATGGNINKAHKIIGAYNNEP
                     ISYKKLKKLKDNLAELSVHQRMEQYQLKSDRADVLVPALEIYCNIMKKLDCAKIHVPK
                     IGLSDGIVYDLHKKHTARK"
     gene            complement(1447..>1765)
                     /locus_tag="PKN22_11380"
     CDS             complement(1447..>1765)
                     /locus_tag="PKN22_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNS43351.1"
                     /translation="INKQRIHKALETAMDVYENRLRKIPTSEFNEVMLDIVHATPPPA
                     VKGKYIKIKYCQQLPTHAPAFVFFCNLPQYIGDPYKRFVENKLRERYDFYGVPIKIFF
                     RKK"
BASE COUNT          475 a          349 c          338 g          603 t
ORIGIN      
        1 cattacttgt ttgcccacaa tttcatccct taaaaatgct atggattgtt cgttttctgc
       61 gctaatcaag tgagtagggt agccaatttg ctcgtacatg tgtgcaagcg cttgggcata
      121 ttccaaatca tcgggggtgt ataaatctat tttgttgatg agcaaggttg ttggaatgcg
      181 aaacgattcg gctgccacta aaaaacggtc gataaacgcc aattgggtag taggattttt
      241 aatagtaatc accaaataag cacgatcaac atttgcagcc aaaatttgca tttgtttact
      301 taaattggtg gatttgcgga caatgtagtt gcttcgtttc tcaaaacctg taatcacacg
      361 attgccttca tcatcaatgt catcggctaa taccacgtta tcgccaactg caatgggatt
      421 ggtggtgctc aacccttcta aacgtatttt tccacggata cggcaattta cgatggaacc
      481 gtcggagagt tccacaatgt accattttcc tgttgatttt aatacttttc ctttcaattg
      541 agcagttttg aagttatttt cgagcggtat gttttttatg caaatcgtac acaattccgt
      601 cagaaagacc gatttttgga acatgtattt tagcgcaatc caattttttc atgatgttgc
      661 aataaatttc caatgccgga accaatacat ctgcacggtc gctttttagt tggtattgct
      721 ccattcgttg gtgcacactc aattcggcta aattgtcttt gagttttttt aattttttat
      781 agctaatggg ctcgttgttg taagcaccaa taattttatg agctttattg atgtttcctc
      841 ccgttgcata aaacgtaatt tcaccgttga tattggcaac gtttttttcc aaccattggt
      901 aaatttcgtc ccaaatagtt gcatttgttt tgccaaataa cacgcgaatg gtaccaattt
      961 caaacgattg agcacgttct tttttaccgt tgatgaacac actaatttcg gtgctaccac
     1021 caccaacgtc caccacaatg taaggattgg ttttttcgag tttcatcaat ttaaaagttc
     1081 cagaaatcaa gcgagcttct tcatcacccg aaatggtttc aacctcaatg ccagttgcgt
     1141 ttaaaatttg ctcttttaca acgctgttat tggaggcatc gcgcatggca gatgtggcac
     1201 acgcacgtaa ttctttaact tcaaacaatt cggcaatcaa ttgaaatgcc ttcatggttt
     1261 ttacaaattg ttcggctttt tttgagttga tggttccgtc gttaaagaca ctttcaccca
     1321 aacgcaaggg aatacgggtg tatgaaacct tgttgatgaa ttttttacca tcgtctttga
     1381 tgacttcacc aatgagtaat cgtgcggcat tggtaccaat gtctattgcg ccaaacttca
     1441 tgctgtttat ttttttctaa agaaaatttt aatcggaaca ccataaaaat catagcgttc
     1501 tcttaacttg ttttccacaa aacgtttgta aggatcgcca atatattgcg gtaagttgca
     1561 gaaaaacacg aatgctggag catgtgttgg caactgttgg caatatttga ttttgatgta
     1621 ttttcctttc acagcaggtg gaggagtagc atgcacaatg tccaacatca cttcattgaa
     1681 ttcagaggta ggaattttgc gcaatctgtt ctcgtaaacg tccatcgctg tttccaacgc
     1741 tttgtgaata cgttgtttgt tgata
//
LOCUS       DAOQVQ010000503         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_89940_length_1751_cov_3.951061, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000503 DAOQVQ010000000
VERSION     DAOQVQ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89940_length_1751_cov_3.951061"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..659)
                     /gene="frr"
                     /locus_tag="PKN22_11385"
     CDS             complement(99..659)
                     /gene="frr"
                     /locus_tag="PKN22_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNS43352.1"
                     /translation="MTEELNDIFNDFKASNEKTLNHLDNELLKVRAGKATPSMLHGVM
                     VDYYGSPTPLQQVANISTLDARTISIQAWEKSMLTEIAKGIMMANLGLNPQNNGETIL
                     IAVPPLTEERRRDLVKKAKAEGEHAKVGIRNNRKDALDFIKDLKESLSEDIIKDAENQ
                     VQTITDTYVKKVDEKIVEKEKDIMTI"
     gene            850..>1751
                     /locus_tag="PKN22_11390"
     CDS             850..>1751
                     /locus_tag="PKN22_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNS43353.1"
                     /translation="MYIKDVCAISPQLTYSDAFVAGDFQLHTSKVMLAKEPNYLDFIP
                     AGLLRRMGKAVRMGIGAGLPLINNHPSIEGVIIGTANGGLEDCIRFLNQIVDYEEGVL
                     TPTNFVNSTPNAVAGQLALMGAKTGYNSTHTNGSLAFDNALLDAQLVLEATHQPMELL
                     VGAIEEISDYNYNIDTLNKHYKTELLSNDQLLASTTDGSICGEGATMFVVSNSAQGAI
                     AEVVDQCQVSFPTQQEVTNLVADLLAKNNLTPQEIDVLLFGNAGDGRTDYWYQHLQQS
                     LFPAKNVHTFKQFCGDYRTASAFG"
BASE COUNT          525 a          305 c          330 g          591 t
ORIGIN      
        1 attgctgtaa taaaggggaa gtcgcatggc ttcctctttt agtttaaaag aggaagccat
       61 gcgacttccc ctttattgct gtaatatgaa tcaatcaatt agattgtcat aatatctttt
      121 tctttttcaa cgattttctc gtctactttc ttcacgtatg tatcggtaat tgtttgaact
      181 tgattttcag catctttgat aatgtcttct gacaagctct ctttcaaatc tttaataaaa
      241 tccaacgcat ctttacggtt gttgcgaata ccaacttttg catgttcacc ctcagccttt
      301 gcttttttaa ccaaatcacg acgacgttcc tctgtcaaag gaggaacagc aattaagatc
      361 gtttctccat tattttgagg gttcaaacct aagttagcca tcataatacc cttagcaatt
      421 tcagtcaaca ttgatttttc ccacgcttga attgaaatgg tgcgagcatc caaagttgaa
      481 atgttggcta cttgttgaag tggagtaggt gaaccataat aatctaccat cacaccgtgc
      541 aacatagaag gtgtagcttt tcctgctctc acttttaaaa gttcattgtc caagtgattt
      601 aaagttttct cgttagaagc tttaaaatca ttaaaaatat cgtttaattc ttcagtcata
      661 ttttttgaat tgctttgcaa atataacaaa tgatacagat aatagaattg atttgttgga
      721 tatttaagtt ttcatttcaa aatatagtga tggttgtttt gatgaaaaga tggattcgat
      781 ttcattttgt taactttgta aatgcgattt tattgaaaat aactatttta gcaccattct
      841 gatataaata tgtacattaa agacgtttgt gccatttcgc cacagttaac ctatagtgat
      901 gcgtttgttg ctggagattt tcagttgcat acatcaaaag ttatgcttgc taaagagcct
      961 aattatttgg attttattcc tgctggattg ttgagaagaa tgggaaaagc tgttagaatg
     1021 ggaattggtg ccggacttcc actcatcaac aatcatccat ccattgaagg ggtgattatt
     1081 gggactgcca atggtggttt ggaagattgc attcgttttt tgaatcaaat tgtggattat
     1141 gaagaagggg tgttgactcc aacaaatttt gtcaacagta cacccaacgc cgtggctggt
     1201 cagctagctt tgatgggggc taaaacagga tataattcta cgcacaccaa cggttcattg
     1261 gcttttgata acgctttgtt agacgcacaa ttggtgttgg aagcaaccca tcagcccatg
     1321 gaattattag tgggcgcaat tgaagaaatt tcagattaca attacaatat tgatacttta
     1381 aacaagcatt acaaaacaga attattatcc aacgaccaac tccttgcatc aacaacagat
     1441 ggttctattt gtggtgaggg cgcaacgatg tttgtagttt ctaatagtgc acaaggagca
     1501 attgcagaag tggttgatca atgtcaagtt tcttttccta cacaacaaga agtaacgaat
     1561 ctagtagcag atttattagc caaaaacaac ttaactccgc aagagataga tgttttatta
     1621 tttggtaacg caggcgatgg tagaaccgat tattggtacc aacatttaca acaatcactt
     1681 tttccagcaa agaatgtgca tactttcaaa caattttgtg gtgattaccg aacagcgagt
     1741 gcttttggat g
//
LOCUS       DAOQVQ010000504         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_90456_length_1745_cov_4.166864, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000504 DAOQVQ010000000
VERSION     DAOQVQ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90456_length_1745_cov_4.166864"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /gene="purH"
                     /locus_tag="PKN22_11395"
     CDS             complement(<1..1445)
                     /gene="purH"
                     /locus_tag="PKN22_11395"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNS43354.1"
                     /translation="MSATKKIKSALISVFDKTGLEPIIKQLHNDGVTIYSTGGTQSFI
                     EGFGIPVERVEDLTSYPSILGGRVKTLHPKVFGGILNRRGLAEDQAQIAEYEIPELDL
                     VIVDLYPFEQTVASGATHEAIIEKIDIGGISLIRAAAKNYQDVVIIPSQAQYGEFLSI
                     ISASNSATTIEQRQAFARDAFHVSSNYDTHIFNYFNNGELDVFKQSIATSQVLRYGEN
                     PHQKGVFYGDLDAMFDKLHGKELSYNNLLDVDAAVALMAEFKNDDPTFAILKHNNACG
                     LATRSTIKEAYEAALAGDPVSAFGGILIANKTIDFATAELVNELFCEVVIAPAYDQKA
                     IELLQSKKNRIILVQKDIELPKKQFRTILNGVLEQDKDLLQETAADLTPRTNKQPTAD
                     EITDLLFANKLVKHTKSNTIVLAKNKTLLASGTGQTSRVDALQQAIHKAKSFNFDLNG
                     AVMASDAFFPFPDCVEIANNAGITAVIQPGGS"
     gene            complement(1512..>1745)
                     /locus_tag="PKN22_11400"
     CDS             complement(1512..>1745)
                     /locus_tag="PKN22_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43355.1"
                     /translation="TGTYDFQLYFDFNGSPLNFIEKGTYHIENKNIAKLVSSDGKNRD
                     MIIVYKTKEDLVLDFPNVDYLNYHLVLKKIKAK"
BASE COUNT          542 a          325 c          295 g          583 t
ORIGIN      
        1 gaaccacctg gctggattac agcagttata ccagcattgt tggcaatttc aacacaatct
       61 gggaaaggga aaaatgcgtc cgaagccatc acagcaccat tcaaatcaaa gttgaacgat
      121 tttgctttgt gaatggcttg ttgcaaggca tccacacgtg atgtttgccc agtaccactt
      181 gccaataatg ttttattttt tgccaaaaca atagtgtttg atttggtgtg ttttaccaat
      241 ttattagcaa acaacaaatc ggtaatttca tccgctgttg gttgtttgtt ggtgcgtggt
      301 gttaaatctg cagcagtttc ttgcaacaaa tctttatctt gttccaacac gccatttaaa
      361 atggtgcgga attgtttttt aggtaattca atatcttttt gaaccaaaat aattctgttt
      421 ttcttgcttt gcaataattc aattgctttt tggtcgtatg ctggagcaat aacaacttcg
      481 cagaacaatt cattaaccaa ttcagcagtt gcaaaatcaa ttgttttgtt ggcaattaaa
      541 attccgccaa aagcacttac agggtcgcct gccaatgcag cttcgtaggc ttctttaatg
      601 gttgagcgtg ttgccaaacc acatgcgttg ttgtgtttta aaattgcaaa tgttggatcg
      661 tcgtttttaa actcagccat caatgctaca gccgcatcaa cgtctaacaa attattgtat
      721 gacaattctt taccgtgcaa tttatcaaac atagcatcca aatcaccgta gaaaacacct
      781 ttttggtgtg ggttttcacc gtaacgcaac acttgagaag ttgcaatact ttgtttaaac
      841 acatccaact caccgttgtt gaagtagttg aaaatatgtg tatcgtaatt ggatgaaaca
      901 tggaatgcat cgcgagcaaa agcttgacgt tgctcaatag ttgttgcact atttgacgct
      961 gaaatgatgc ttaaaaattc gccatattgt gcttgcgaag gaataatcac tacatcttgg
     1021 taattttttg cagcagcacg aatcaatgaa attccaccga tatcgatttt ctcaataatt
     1081 gcttcatgag tagctccaga tgctactgtt tgctcaaaag gatataaatc aacaatcact
     1141 aaatccaatt ctggaatttc gtattctgca atttgtgctt gatcttctgc caaaccacga
     1201 cggttcaaaa tcccaccaaa aacttttgga tgcaaggttt taacacgacc tcctaaaata
     1261 gaaggataag acgttaaatc ttccactctt tctactggaa taccaaatcc ttcgatgaac
     1321 gattgtgttc cacctgttga ataaattgtt acaccatcgt tgtgtaattg cttgataatc
     1381 ggctctaaac ctgttttatc aaatactgaa attaatgctg atttaatttt ttttgttgca
     1441 ctcatgaaat ctaactaaaa agaaagtgca aaattagcta atttttaaaa gaaacttggg
     1501 aaaaaaacaa attattttgc ctttattttt ttgagcacca aatggtagtt gagataatca
     1561 acatttggaa aatcaagaac taaatcttct ttggttttgt aaacaataat catatctctg
     1621 tttttaccat cggaagaaac taattttgca atatttttat tttcaatgtg ataggttcct
     1681 ttttcaataa aattcaacgg acttccatta aaatcaaaat acaattgaaa atcataggtt
     1741 ccggt
//
LOCUS       DAOQVQ010000505         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_90782_length_1741_cov_4.308422, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000505 DAOQVQ010000000
VERSION     DAOQVQ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90782_length_1741_cov_4.308422"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1699
                     /locus_tag="PKN22_11405"
     CDS             155..1699
                     /locus_tag="PKN22_11405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNS43356.1"
                     /translation="MEQREDKLKEVISHAKEYGFIFPSSEIYDGLAAIYDYGQLGSEL
                     KNNIKQYWWKSMVQLHENIVGIDASIFMHPTTWKASGHVDAFNDPLVDNKDSKKRYRA
                     DVLIEEHIEKYNQKNAKEVEKAKKKFGDAFDEAQFLATNPNVVRNNQKIKEIEDRMRE
                     TLENNDLEGVYNLIVDLGIVCPISGTANWTEVRQFNLMFSTELGSVAGDASKIYLRPE
                     TAQGIFVNFLNVQKSGRMKIPFGIAQIGKAFRNEIIARQFIFRMREFEQMEMQFFVRP
                     GEEMTWYNYWKETRKKWHLALNLGEENYRFHDHIKLAHYANAATDIEFNFPMGFKELE
                     GIHSRTDFDLKQHEQYSGKKLQYFDPELNQNYVPYVVETSVGLDRLFLSILSSALKNE
                     VLEDGSERVVLSIPPALAPYKVAVMPLTKKDGLPEKAQEIYNSLRLDFNCQYDEKDSP
                     GKRYRRQDAIGTPYSVMVDHQTLEDNTVTIRDRDTMLQERVAIADLRQKIDEKVNLRS
                     LLNQLV"
BASE COUNT          591 a          281 c          341 g          528 t
ORIGIN      
        1 taaaggttaa taaatacaaa aaagtcggaa gaaatttctt ccgaccattc gtgcttttta
       61 ggtctccttt ttttattcac taccttgcaa tccttgctgt tttaatcagt tatattctct
      121 atatttgcac cttaaatttc attgatattt caccatggaa caaagagaag ataaattaaa
      181 agaagttatt tcgcacgcaa aagagtatgg attcattttt ccatcatctg aaatttatga
      241 tggccttgca gcaatctacg attacggtca attaggttct gaattgaaaa acaacattaa
      301 acaatattgg tggaaatcaa tggtgcaatt gcacgaaaac attgttggaa ttgatgcatc
      361 aatttttatg cacccaacaa cttggaaggc atctggacac gtggatgcgt ttaacgaccc
      421 attggtggat aacaaagatt ctaaaaaacg ttaccgcgcc gatgtgttaa ttgaagaaca
      481 cattgaaaaa tacaatcaaa aaaatgccaa agaagttgaa aaagctaaaa agaaatttgg
      541 tgatgctttt gacgaagctc agtttttagc aaccaaccct aatgttgtgc gcaacaatca
      601 aaaaattaaa gaaattgaag atagaatgcg cgaaacgttg gaaaacaacg atttggaagg
      661 tgtttataat ttaattgttg atttgggaat tgtttgccct atttctggaa cagctaattg
      721 gacagaagta cgtcaattca acttaatgtt ctctacagaa ttaggctcgg tggcaggtga
      781 tgcttctaaa atttatttgc gtccagaaac agctcaaggt atttttgtaa acttcttgaa
      841 cgttcaaaaa tcggggagaa tgaaaattcc gtttggaatt gctcaaatcg gtaaagcgtt
      901 tagaaatgaa attattgcac gtcaatttat tttccgtatg cgtgagtttg aacaaatgga
      961 aatgcaattt tttgtgcgtc caggcgaaga aatgacgtgg tacaattact ggaaagaaac
     1021 acgtaaaaaa tggcacttgg cgttgaattt aggagaggaa aattaccggt ttcacgacca
     1081 tatcaaattg gctcactatg ccaacgctgc aacagatatt gaattcaact tcccaatggg
     1141 ctttaaagaa ttggaaggta ttcactctcg cactgatttt gacttgaaac aacacgaaca
     1201 atattcgggt aaaaaattac agtattttga tccagagtta aatcaaaact acgttccgta
     1261 tgtggttgaa acttcggttg gtttggatcg tttgttttta tctattttaa gttctgcttt
     1321 gaaaaatgaa gtgttggaag acggttcaga acgtgttgtg ttgtccattc cacctgcatt
     1381 ggctccctac aaagttgctg taatgccatt gactaaaaaa gatggattgc cagaaaaagc
     1441 acaagaaatt tacaattctt tgcgtttgga tttcaattgc caatacgacg aaaaagattc
     1501 gccaggaaaa cgttatagaa gacaagatgc cattggtacg ccttattctg taatggtaga
     1561 tcaccaaacg ttggaagaca atacagtaac catccgcgat agagatacca tgcttcaaga
     1621 gcgtgttgcc attgcagatt tacgccaaaa aattgatgag aaagtaaatc ttagaagttt
     1681 attaaatcag ttagtttaac taaaaataga tttttgtaaa aggcattgga gtttatctga
     1741 t
//
LOCUS       DAOQVQ010000506         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_91191_length_1736_cov_2.925640, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000506 DAOQVQ010000000
VERSION     DAOQVQ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91191_length_1736_cov_2.925640"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKN22_11410"
     CDS             complement(<1..541)
                     /locus_tag="PKN22_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNS43357.1"
                     /translation="MRGLKRFFTAVVASVVALSANAQDPSFTQFYSNPIYLNPALAGT
                     HGCARFAANYRNQWPSLSGNYVTYALGYDQYFKQISGGIGVLATHDMTGKATIQTSML
                     GLIYSYHLNVTRKFSLLFGARAAWYQKFLDWDKLTFGDMIDPRKGFIYQTGDVPHGGS
                     KGFFDASAGIVGYSDIFYFG"
     gene            706..>1736
                     /locus_tag="PKN22_11415"
     CDS             706..>1736
                     /locus_tag="PKN22_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43358.1"
                     /translation="MKYFFKTSTPFHSILAFFVILFLYSTSFSQDQKSITIKWSEPLQ
                     QEVNGIVTSLPNFEGQIYAEGEIYYQFIEEVAPKYDANLTLVDVQTGSATEKEIAYLK
                     NNNISIPTQAKWKAKVTNGGKKRLLLFSIVPYIQKDGKIQRITSFTFNQQQIATFSAN
                     KVKSFASESVLKPGSGEWFKIGVTQDGIYKISKQFLAQLGVDVNTINPQHINIYGNGD
                     GVLPESNSKPKTDDLALNAIFVAGESDGSFDENDYILFYAKGPHRWDYNSSTNLFENK
                     RNIYSDLSYYFININAGVAPSRVQNIASSPATPTHTVVSYSYREVHETDVKSLTKSGQ
                     RWYGEEFDA"
BASE COUNT          619 a          322 c          276 g          519 t
ORIGIN      
        1 ctccaaaata aaaaatatca gagtaaccta caattccagc ggatgcgtca aaaaatcctt
       61 ttgaaccacc atgtggaacg tcacccgttt gataaataaa ccctttgcga gggtcaatca
      121 tatctccaaa agtcaattta tcccaatcca aaaacttttg ataccatgca gcacgcgctc
      181 caaaaagcaa agagaatttt cgtgttacat ttaagtggta tgaataaatc aatcctaaca
      241 ttgatgtttg aatggttgct ttccccgtca tatcatgtgt tgctaaaaca ccaataccac
      301 ccgaaatttg cttgaaatat tgatcgtatc ctaatgcata agttacgtaa ttccctgaca
      361 aacttggcca ttgatttcta taatttgcag caaaacgagc acaaccatgt gttccagcca
      421 aagcaggatt caaataaata gggttggaat aaaactgcgt aaatgaaggg tcttgtgcat
      481 tcgcactcag cgccacaaca cttgctacaa cagccgtaaa aaatcgtttc aatcctctca
      541 tttattaaga attagtaaaa tttagtagta agaaaatgta tctcactttg tctttttaac
      601 gtcaatttgt taaaaaggtt acaaatatta taaattacaa ataataaaca aagaaaataa
      661 tcgttttttt atttcaatca aaaaggaaat taacatattt gtactatgaa atattttttt
      721 aaaacaagta ccccttttca ttcaatatta gcgttttttg ttattctttt cttgtattct
      781 acttcatttt cacaagacca aaaatcaatt acaataaaat ggagtgaacc tttgcaacaa
      841 gaagtaaatg gaattgtaac ttcattacct aattttgaag gtcaaattta tgcagaaggt
      901 gaaatttact atcaatttat tgaagaagtt gccccaaaat acgatgcaaa ccttacttta
      961 gtagatgttc aaactggaag tgcaactgaa aaagaaattg cttatttaaa aaacaacaat
     1021 atttcaattc caacccaagc caaatggaag gcaaaggtca caaatggtgg aaaaaaacgt
     1081 ttgttattat tttcaattgt tccctacatt caaaaagatg gtaaaattca acgcattacc
     1141 tcttttactt ttaatcagca acaaatagca acattttcag ccaataaagt aaaatctttt
     1201 gcctctgaat cggtattaaa acctggaagt ggcgaatggt ttaaaattgg tgtaacgcaa
     1261 gatggaatct ataaaatcag taaacaattt cttgcgcaat taggagtgga tgtcaacacc
     1321 attaatccgc aacacattaa tatctacgga aatggtgatg gggtattacc cgaatctaat
     1381 tcgaaaccaa aaactgatga tttggctctc aatgccatct ttgttgcagg cgaatcggat
     1441 ggtagttttg acgaaaatga ttacatttta ttttatgcca aaggtccaca ccgttgggac
     1501 tacaactcga gcaccaatct atttgaaaac aagcgcaata tttattcaga tttatcctat
     1561 tatttcatca atataaatgc tggagtagct ccatcgcgcg tgcaaaacat tgcatcatca
     1621 ccggcaactc ctacacatac ggtagttagc tattcgtatc gtgaggtaca tgaaaccgat
     1681 gtgaaaagct taaccaaatc ggggcaacga tggtatggag aagagtttga cgcaca
//
LOCUS       DAOQVQ010000507         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_91361_length_1734_cov_3.959500, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000507 DAOQVQ010000000
VERSION     DAOQVQ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91361_length_1734_cov_3.959500"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1324
                     /gene="lysA"
                     /locus_tag="PKN22_11420"
     CDS             155..1324
                     /gene="lysA"
                     /locus_tag="PKN22_11420"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNS43359.1"
                     /translation="MTTLEQIKGIETPFYLYDLQLLQQTLQQAKEAADKRNYHVHFAY
                     KANFNDVILRKMHQMGFGADCVSGNEVKKAIEIGVAPENITFAGVGKTEKEIRFALQH
                     AIFAFNVESIQELIVIGEIATDMQQKAHIALRINPNVDAQTHHYITTGLDENKFGILP
                     SNVAECVSIIRMNPFLELQGIHFHIGSQILEMNVFKSLCVKVNEWNSWFEERGVYLKT
                     INVGGGLGVDYKHPDEHPITDFETYFSIFENFLEIRPHQEVHFELGRALVAQCGSLVT
                     QVQYVKTGIKKRFAVVDAGMTELIRPALYQAYHKIEKMTSSNDSTMLYDIVGPICESS
                     DTFAKEIELPALSRGDLLIIRTAGAYGEVMASRYNLRDAIQSYFINDSTPILANG"
     gene            complement(1282..>1734)
                     /locus_tag="PKN22_11425"
     CDS             complement(1282..>1734)
                     /locus_tag="PKN22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF020691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HNS43360.1"
                     /translation="NCIQITSKRLNKVNLLAEEVGKGNIKKLKKFQLNTLTTQEWMSF
                     IRNLPMEVSAKTMKQIDEQLNFKGCNNSEIMCEWYLLAIKRGYTLAQPNMEKFLRKTG
                     RMKYIEPIYEELYHSRYSGDKDLALKIYATAKSNYHPLARIGVESLMK"
BASE COUNT          541 a          305 c          325 g          563 t
ORIGIN      
        1 tatgccgctc aaatttttga aggaataaaa catttaccta tccgcatgat ttcttatggt
       61 ggttccaatc acaacgtgtc acttttagta gattctaaaa acaaagcaga ggcacttcaa
      121 tcacttcaca atcatttgtt ttaatatgtt tgacatgaca acacttgagc aaataaaagg
      181 aattgaaaca cctttttatc tgtatgactt gcaattgttg cagcaaacat tgcaacaagc
      241 aaaagaagca gccgacaaac gcaattacca cgttcatttt gcctataaag ccaattttaa
      301 tgacgtgatt ttgagaaaaa tgcatcaaat gggttttgga gccgattgtg tgagtggcaa
      361 cgaagtgaaa aaagccatcg aaattggtgt tgctcccgaa aatattacgt ttgcaggagt
      421 cggtaaaact gaaaaagaaa tccgttttgc tttgcaacat gctatttttg cattcaacgt
      481 tgaatcaatt caagagctaa ttgttattgg tgaaattgct accgatatgc aacaaaaagc
      541 acacattgca ctgcgcatta atcccaatgt tgacgcacaa acgcatcatt acattacaac
      601 tggtttagac gaaaataaat ttggaatttt accaagcaat gtggccgaat gtgtttccat
      661 tattcgcatg aatccgtttt tagaattgca aggaattcat tttcacatcg gttcacaaat
      721 tttagaaatg aatgttttta aaagtttgtg cgtgaaagtg aatgaatgga acagttggtt
      781 tgaagaacgt ggtgtttatc ttaaaacgat caatgttggt gggggattgg gtgttgatta
      841 caaacatcca gacgaacatc ccattaccga ttttgaaact tacttttcaa tttttgaaaa
      901 ctttttagaa atacgtccac accaagaagt acattttgaa cttggtaggg cattagtggc
      961 tcaatgcggt agtctggtga cgcaagttca atatgttaaa acaggaataa aaaaacgttt
     1021 tgccgttgtg gatgcaggaa tgacagagtt gattcgccct gcactatacc aggcttatca
     1081 caagattgaa aaaatgactt cttcaaacga tagcacgatg ttgtatgata ttgttggacc
     1141 catttgtgaa agctcggata cttttgcaaa agaaattgag ttgccagctt tgtctcgagg
     1201 tgatttatta ataattcgca cagcaggagc ttacggagaa gtaatggctt cacgttacaa
     1261 tttacgcgat gcaattcaaa gttatttcat caacgattct acgccaattc tagccaacgg
     1321 atgataattg ctttttgccg ttgcataaat ttttagtgcc aaatctttat ctccagagta
     1381 acgactgtgg tacaattcct cgtaaatagg ttcgatgtat ttcatacgtc cagttttacg
     1441 taagaatttt tccatgttag gttgcgccaa tgtataacct cgtttaatag ctaataaata
     1501 ccactcacac atgatttcgg aattattgca gcctttgaaa ttcaattgct cgtcaatttg
     1561 tttcattgtt ttagcactca cttccattgg taagttgcga ataaacgaca tccattcttg
     1621 ggttgtgagg gtatttagct gaaatttttt caatttttta atgtttcctt tcccaacttc
     1681 ttcggccaat aaattcactt tattcaatcg ttttgaagta atttgaatac agtt
//
LOCUS       DAOQVQ010000508         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_91969_length_1727_cov_4.595096, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000508 DAOQVQ010000000
VERSION     DAOQVQ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91969_length_1727_cov_4.595096"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PKN22_11430"
     CDS             <1..1040
                     /locus_tag="PKN22_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43361.1"
                     /translation="GVESAVVIGVLDQADTTHYIKITRTFIGDGQTSAVDIAKISDSS
                     YFDKVEVTIKEILSNGSVGRTFVLDERILHNKTTDGAFYAPDQKVYAFDTPANQPLLE
                     DARYQMTAKIDDGRIVITGETKLVSGITVSNVGNTNYAIKLTQSGSSLGQYANQSIQV
                     SNVGSSYKLNARVRFDYREFTTGLLDSTDHSIWFELGEYDVQPGASSSQTFTFSGETF
                     YKTLAEKIPVSASIEKRINTGFEFFFTGASSELSNYIEVNKPSSSLAQTKPTFTNLKI
                     TEGHNVIGILAARQTVRYYKPATAASSIVQTLDKKSRRELCIGPKTGNLSFCSRHVAD
                     QAPVQETWYCN"
     gene            1046..>1727
                     /locus_tag="PKN22_11435"
     CDS             1046..>1727
                     /locus_tag="PKN22_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNS43362.1"
                     /translation="MDERITLFVDVILPLPVHREFTYRVPFELNENIQEGVRVVVPFG
                     KSKFYTGVVTKVHSNVPSDYTAKYVEHILDEAPIINGNQYVFWKWIAKYYMAPIGDVL
                     NAALPANFKLASETKVVLHPDFDAQLELAERELEIVETLEVREELTLKELSEILSIKN
                     VQPLVKRMIDKCIVITQEELNQKYTPKKESFVAINPVFSEEELNDVLTTLENNKSKAS
                     QFDGLLLLL"
BASE COUNT          575 a          298 c          339 g          515 t
ORIGIN      
        1 aaggtgtaga atctgccgtt gttattggtg ttttagatca agctgataca acgcattaca
       61 tcaagattac tcgtacattt attggtgacg gacaaacaag tgctgttgat attgcaaaaa
      121 tttctgattc gtcttatttt gataaagttg aagtaaccat aaaagaaatt ctttcaaatg
      181 gttcggtagg tagaacgttt gttttagacg agcgcatttt gcacaacaaa actacagatg
      241 gagcttttta tgcacccgat caaaaagtgt atgcgtttga taccccagcc aatcaaccgt
      301 tgttagaaga tgcacgttac caaatgactg caaaaattga tgatggaaga attgtaatca
      361 ctggtgaaac aaaattagtg agcggaatta ctgtatccaa cgttggaaac accaattatg
      421 cgattaaatt aacgcaatct ggaagtagtt tgggacaata tgctaatcaa tctattcaag
      481 tttcaaatgt tggttcttca tacaaattaa atgctagagt aagatttgat taccgtgaat
      541 ttaccactgg attgttagat tcaaccgatc attctatttg gtttgaattg ggtgaatacg
      601 atgtgcaacc aggtgctagc agctcacaaa cgtttacatt ttcgggtgaa acgttttaca
      661 aaacattggc cgaaaaaatt ccagtttcag catctattga aaaaagaatt aatactggat
      721 ttgagttctt ctttacagga gcgagcagtg aattatctaa ctatatcgaa gttaacaaac
      781 cgtcatcatc tttagcacaa acaaaaccta cgtttaccaa cttgaaaatt acagaggggc
      841 acaacgtaat tggtattttg gcagctcgtc aaacagtgcg ttattacaaa ccagcaacag
      901 ctgcatcaag catcgttcaa acattggata aaaaatcgcg cagagaattg tgcataggtc
      961 caaaaacagg aaatttaagt ttttgttcgc gccacgtagc agatcaagcg cctgtgcaag
     1021 aaacatggta ttgtaactaa ttgaaatgga tgaacgcatt accctttttg ttgatgtaat
     1081 tttaccactt cctgttcata gggaattcac ctatcgtgtt ccatttgaac tgaatgaaaa
     1141 tatccaagaa ggcgttcgtg ttgttgttcc gttcggaaaa tcaaagtttt atacaggtgt
     1201 tgtaaccaag gtgcattcca atgtgcctag cgattatacc gccaaatatg tggagcacat
     1261 tttagatgaa gcccccatta tcaatggtaa tcaatacgtt ttttggaaat ggattgctaa
     1321 atattacatg gcgccaattg gtgacgtgct taatgctgct ttgcctgcca attttaaact
     1381 ggcaagtgaa acaaaagtag tgttgcaccc cgattttgat gctcaacttg aattggcaga
     1441 acgtgaattg gaaatcgttg aaaccttgga agttcgagaa gagttgacac tcaaagaatt
     1501 atcggaaata ttatcgatta aaaatgtgca acctcttgta aaacgaatga tagataaatg
     1561 cattgttatt acccaagagg agcttaatca aaaatatacg ccaaaaaaag aaagttttgt
     1621 tgccatcaac cctgtttttt cggaagaaga actcaatgat gtgctaacaa cacttgaaaa
     1681 caataaaagc aaagcatcgc aatttgatgg tttgttattg ctcttaa
//
LOCUS       DAOQVQ010000509         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_92041_length_1727_cov_2.038278, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000509 DAOQVQ010000000
VERSION     DAOQVQ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92041_length_1727_cov_2.038278"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..521
                     /gene="rsfS"
                     /locus_tag="PKN22_11440"
     CDS             162..521
                     /gene="rsfS"
                     /locus_tag="PKN22_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNS43363.1"
                     /translation="MKKEDKKLLEAIVAGMQERKAKRVVTVDMTKLDAPCQYFVICEG
                     DSNVHVSSIAMEVKDYVRKNASEKPFATDGFENCEWIALDYGSVIVHVFRRETRAFYD
                     IEHLWEDADIKEYEDVV"
     gene            659..>1727
                     /locus_tag="PKN22_11445"
     CDS             659..>1727
                     /locus_tag="PKN22_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNS43364.1"
                     /translation="MEQKKNSKPQLPNPKQFKPKFNFYWIYLAIAVFFAASLFWDGGQ
                     TSKEVPYSKLEEGLRSGCVKSMTVTITQNKVDAEIDPKCAEQLFGKTYKEGDRIPNIN
                     ALVPSVEEFSKLITELQKEKVFTGEVSYKEGKNYMEMFLYSILPILLLVLFFVFMNRK
                     MSGGAGGAGGVFNVGKSKAQLFDKGDATKAKTTFKDVAGLAGAKQEVEEIVAFLRKPD
                     KYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEADVPFFSMSGSDFVEMFVGVGASRV
                     RDLFRQAKEKAPCIVFIDEIDAVGRARGKNNNMGGNDERENTLNQLLTEMDGFGSNSG
                     VIILAATNRADILDKALLRAGR"
BASE COUNT          512 a          242 c          415 g          558 t
ORIGIN      
        1 ttccatgtta ttaggaaaat ttttgcgtta tgtggtttgg atgtatgtca atggcctgtt
       61 tttttagata aaactgccaa aaagtctggt tttaggattt ggaatagaat gtgtatattt
      121 gtgaagtgga attttacgaa cagaatgaaa taaatttttg aatgaaaaaa gaagataaaa
      181 agttacttga agctattgta gctggtatgc aagagagaaa ggcaaaacgt gttgttacgg
      241 ttgatatgac caagttagat gctccttgtc agtattttgt tatttgcgaa ggtgattcga
      301 atgtgcacgt gtcttccatt gctatggaag tgaaagatta tgtgcgtaaa aatgccagtg
      361 aaaaaccgtt tgctaccgat ggctttgaaa attgtgaatg gattgcgttg gattatggtt
      421 cagtcatagt gcatgttttc cgtcgtgaga cacgtgcatt ttacgatatt gaacatttgt
      481 gggaagacgc tgatattaaa gaatacgaag acgttgttta acgaaaattc gatttaagct
      541 tgtctagatt ttacgcgagt tggtcattta tataacctat ttcttttgct aggttttgcg
      601 tggtcgctca cagattatgt gtgaatttag tttgatgtaa ttattgagag tataaaatat
      661 ggaacagaaa aaaaatagta aaccgcaatt gcccaaccct aagcaattta agcctaaatt
      721 caatttttat tggatttatt tggctattgc cgtgtttttt gccgcgtctc ttttttggga
      781 tggagggcaa acttctaagg aagtgcctta ctcaaaactt gaggaaggat tacgtagtgg
      841 atgtgtaaaa agtatgaccg taaccattac acaaaacaag gttgatgcag aaattgatcc
      901 aaagtgtgca gaacaattat ttggaaaaac ttataaagaa ggcgatagaa tacctaatat
      961 caatgcgttg gttccttctg tggaggaatt ttcaaaattg attaccgaac tccaaaagga
     1021 aaaggtattt acgggtgaag tatcgtacaa ggaaggtaaa aactacatgg agatgtttct
     1081 ctattccatt ttgccaattt tgttgctagt acttttcttc gtctttatga atcgtaaaat
     1141 gtcgggtggt gccggtggtg ctggaggcgt ttttaatgtt ggtaaatcta aagcgcaact
     1201 tttcgataaa ggtgatgcta ccaaagctaa aaccacgttc aaggatgtag caggattagc
     1261 tggtgctaaa caagaagtag aagagattgt ggcttttttg cgtaaacctg ataaatatac
     1321 cgaattggga ggtaaaattc ctaaaggcgc tttacttgta ggccctccag gaactggtaa
     1381 aacattgtta gcgaaagccg ttgctggcga agcagatgtg cccttctttt cgatgtcggg
     1441 ttccgatttt gtggaaatgt ttgtaggtgt tggtgctagt cgtgtacgcg atttgttccg
     1501 tcaagccaaa gagaaagcgc catgtattgt gtttatcgac gaaattgacg ctgtagggcg
     1561 tgctcgtgga aaaaataata acatgggtgg taatgatgaa cgtgaaaaca cattgaatca
     1621 attacttaca gagatggacg gttttgggtc taatagtggt gttattattt tagcggctac
     1681 caatcgtgct gatattttgg ataaagcctt gttgcgtgca ggtcgtt
//
LOCUS       DAOQVQ010000510         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_92359_length_1723_cov_3.263189, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000510 DAOQVQ010000000
VERSION     DAOQVQ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92359_length_1723_cov_3.263189"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKN22_11450"
     CDS             <1..319
                     /locus_tag="PKN22_11450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNS43365.1"
                     /translation="IFGSGEDGITVVNGAIFQNYFDLLTNINDQQQLLKQIKARGYAA
                     NSDHYWFTEKGVPSFFIYTMGPNKNYHDIDDTYENLSFAEVNDLQTLLVNFVKQLDSV
                     IGK"
     gene            607..>1723
                     /gene="sufB"
                     /locus_tag="PKN22_11455"
     CDS             607..>1723
                     /gene="sufB"
                     /locus_tag="PKN22_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNS43366.1"
                     /translation="MGYTEEELAEDLKNQEYKYGFTTDIESDDAPKGLDEDIIRLISK
                     KKDEPEWMLEYRLNAYRTWLTMEEPSWAHVSFEKPNYQDIIFYSAPKQATKAKTWEEV
                     DPELKETMNKLGISLEEQKRLQGVAVDFVMDSVSVATSFKEKLKELGIIFCSFSDAVQ
                     EHPELVKKYMSTVVPVTDNYYAALNSAVFSDGTFCYIPKGVRCPMELSTYFRMNKSGT
                     GQFERTLVVADEGSYCSYLEGCTAPQRDENQLHAAVVELIAMENAEIKYSTVQNWYPG
                     DKNGKGGVFNLVTKRGLCERNAKISWTQVETGSAVTWKYPSCILKGDNSIGEFYSVAV
                     TNHFQQADTGTKMIHIGKNSKSRIVSKGISIGKSQNTY"
BASE COUNT          606 a          267 c          340 g          510 t
ORIGIN      
        1 catctttggc agtggcgaag acggcataac ggttgttaac ggtgccatat ttcaaaacta
       61 ttttgattta ttaaccaata ttaacgacca acaacaactt ttaaaacaaa taaaagcacg
      121 tggatatgct gctaattccg atcattattg gtttacagaa aaaggtgttc cttcgttttt
      181 tatctatacg atgggtccca acaaaaacta ccacgatata gacgacactt atgaaaatct
      241 ttcgtttgca gaagtgaatg atttgcaaac cttgttggtg aattttgtaa agcagttgga
      301 ttccgttatt gggaaataaa aaaattcata actatttaga attgttctaa acaacaaaca
      361 tctatcttgc tgatttacaa caatataatt gtaaataaaa aaattaattt ttacgaaatt
      421 gacttttatc agtttttaaa tcaaaaaaat atcttttgat aactttgttc atataccaca
      481 aaaaaggtat ttttgcggag taatttagat tttatttaaa actattctaa ataacaacca
      541 aagaaaaatt aggcaatata gattaccaca gcgttgatat acagcgctta acggaagttt
      601 tacaatatgg gttatacaga agaagaatta gcggaagacc tcaaaaacca ggaatacaaa
      661 tatggtttca ccaccgatat tgagtcggat gatgcaccaa aagggttgga tgaggacatt
      721 attcgtttaa tttcaaagaa aaaagatgag ccagaatgga tgttagagta ccgtttgaat
      781 gcttacagaa catggctaac gatggaagaa ccaagttggg cacatgtttc gtttgaaaaa
      841 ccaaactacc aagacatcat tttttactcg gcaccaaagc aagctacaaa ggctaaaacg
      901 tgggaagaag tggatccaga gttgaaagaa accatgaata aattgggtat ttctttggaa
      961 gaacaaaaac gtttgcaagg tgtggcggtt gactttgtga tggactctgt atccgtggca
     1021 acatctttta aagaaaaatt aaaagaattg ggcattattt tctgttcgtt ttcggatgcg
     1081 gtgcaagaac atccggaatt ggtgaaaaaa tacatgagca ctgttgttcc agttaccgat
     1141 aattactacg ctgcattaaa ttcggctgtc ttttcggatg gaacgttttg ttatattcca
     1201 aaaggtgttc gttgcccgat ggaactttca acctatttta gaatgaacaa atcaggtacg
     1261 ggtcagtttg aacgcacctt ggttgttgcc gatgaaggtt cgtattgttc gtatttggaa
     1321 ggatgtactg cacctcaacg cgatgaaaat caattgcacg cagctgtagt tgaattaatt
     1381 gcaatggaaa atgcagaaat taaatattct acagttcaaa actggtatcc tggagataaa
     1441 aacggaaaag gtggggtttt caacttagtt accaaacgtg gtttgtgcga aagaaatgca
     1501 aaaatttcgt ggacacaagt agaaacaggt tcggcagtta cttggaaata tccttcgtgt
     1561 attttgaaag gcgacaattc tataggtgag ttttattcag tagctgtcac aaatcatttt
     1621 cagcaagctg atacaggaac aaaaatgata catattggca aaaactcaaa aagtagaatt
     1681 gtatccaaag gtatcagtat tggaaagagt caaaatacct ata
//
LOCUS       DAOQVQ010000511         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_93060_length_1715_cov_3.672892, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000511 DAOQVQ010000000
VERSION     DAOQVQ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93060_length_1715_cov_3.672892"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1715
                     /locus_tag="PKN22_11460"
     CDS             28..>1715
                     /locus_tag="PKN22_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNS43367.1"
                     /translation="MKFTIESNNLALVDVVNNISLQEIDYIKKYSGSGNVDFSLVVDE
                     NRLDEIAPSIDCKFNVSNGKLIEPTQNIQVNNLSLKGEFSSKNGAGNEYLSLEDIRFT
                     NTTGPFSGNIRISEFNAPLFQGNANGNIDLAIAHSLFHIPMVEKMNGNMNINADFIVK
                     MLEADEEIGTNFNIIKSEGAVELTNCSVQLKDDQRNFHSINGKITLNNNDVNVAGLGV
                     QLQSSDLLLNGTFTNLIGYFRGDGLLQATMDVRSNKIVVEDLATTSKEEQIQTQAPRS
                     YMLPQQIDGKLALSIGQLSYDHHHFEKINGNLSLTEHTLRFEGINFVTSGAAVNGSIL
                     IREQSEEFFQTSSTLSTANVQLKQLMKDWNNFNQQVITADNIFGKATAQLQLDAPFDL
                     RTGIQMKNVKSNIYLKIENGQLKNVGAFKDIIQSANNSSSAKLVIGANNIKTFGEKLS
                     DLRFETLENQITIQNGVITVPEMKIQSSALNIVASGTHTIEQKIDYRFAFRLRDLKAA
                     KDTEFGEVVDDKTGLIVYLRMYGDLSNPQFSWDKEAKAEARKEYNEQEKQTLKS"
BASE COUNT          606 a          284 c          338 g          487 t
ORIGIN      
        1 ttttcgggaa atgtgaaccc ttcaaccatg aagtttacta tagaatctaa caacttggcg
       61 ttggtggatg tggtgaacaa tatttctctg caagaaatag attacattaa aaaatattcg
      121 ggttctggaa atgtggattt ctcattggtt gttgacgaaa acagattgga tgaaattgcc
      181 ccatcaattg attgtaaatt caacgtatcg aacgggaaat tgattgaacc aactcaaaat
      241 attcaagtaa acaacttgag tttaaaaggt gaattttctt ctaaaaatgg tgctgggaat
      301 gaatatttat cactagaaga cattcgtttt accaacacca caggtccatt ttcgggaaat
      361 ataagaatta gtgaattcaa tgctcctttg tttcaaggaa atgcaaatgg aaacattgat
      421 ttggcaatag cacattcact ttttcatatt ccgatggttg aaaaaatgaa cggcaacatg
      481 aacattaatg ccgattttat tgtgaaaatg ctggaagccg acgaagaaat tggtactaat
      541 ttcaatatta ttaaaagtga aggtgcggta gaattgacaa attgctctgt tcaactgaaa
      601 gacgatcaac gtaattttca ttctatcaat ggaaaaatta ctctcaacaa caacgatgtg
      661 aatgtggctg gattgggagt tcaattgcaa tcgtcggatt tgttgcttaa cggaactttt
      721 accaacctca tcggttattt tagaggggac ggacttttgc aagccactat ggatgtgcgc
      781 agcaataaaa ttgtagtaga agacttagca acaactagca aggaagagca aatacaaaca
      841 caagcaccac gcagttatat gttgcctcaa caaattgatg ggaaactagc actctccatc
      901 gggcaattga gttatgacca ccatcatttt gaaaaaataa acggcaacct ttcgttaaca
      961 gaacatacgt tacgctttga aggtatcaat tttgtaactt caggagctgc tgttaacggt
     1021 tctattttaa ttcgcgaaca atccgaagaa tttttccaaa catcaagcac gttgagtact
     1081 gcaaacgtgc aattaaaaca attgatgaaa gattggaata attttaatca gcaagtgatt
     1141 actgccgata atatttttgg aaaagcaact gctcaactgc aattagacgc tccatttgac
     1201 ttgcgaacag gtattcaaat gaaaaacgtg aagtcgaaca tctaccttaa aatagaaaat
     1261 gggcaattga aaaacgtagg tgcgtttaaa gatattattc agagtgccaa taattcatcc
     1321 tcggcaaaat tggtcattgg cgccaataac attaaaactt ttggtgaaaa actatctgat
     1381 ttacgttttg aaaccttgga aaatcagatt accattcaaa atggagtaat tacagtgccc
     1441 gaaatgaaaa tacaatcttc tgcgttgaac attgtggcta gtggtacgca taccattgaa
     1501 caaaaaatag attaccgttt tgcattccgt ttgcgcgatt tgaaagcagc aaaagatact
     1561 gaatttggcg aagtggttga cgacaaaaca gggcttattg tttatttaag aatgtatggc
     1621 gatttgagta atccgcaatt tagttgggac aaagaagcaa aagcagaggc gcgcaaagaa
     1681 tacaacgagc aagaaaaaca aactttaaaa agcat
//
LOCUS       DAOQVQ010000512         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_93812_length_1707_cov_2.515738, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000512 DAOQVQ010000000
VERSION     DAOQVQ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93812_length_1707_cov_2.515738"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PKN22_11465"
     CDS             complement(<1..1327)
                     /locus_tag="PKN22_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS43368.1"
                     /translation="MTPTRLFDLVYNQLNDYPTDQMFVTKRNGKWEAISTKEFVEQTK
                     LVSRGLIALGVQTGDRIAVVSSNRVEWNIMDIAIQQIGAIMVPIYPNISENDYNYIFN
                     NAGIKLSFVGTEDLYQKISRIKSNVPSLENTYTFDVVNGAPNWKEVAQLADSIDEAEI
                     VKRSDAIKNDDIVTIIYTSGTTGNPKGVMLTHNNILSNALACESRIPADRYSRVLTFL
                     PVCHVYERMLHYLYIRMSASVYFAESLDTIGENIKEVKPDVFTAVPRLLEKVFDKIIA
                     KGEELTGIKRKLFFWAVELGEEYDVIGKSAWYHFKLSIARKLIFSKWQEALGGQVKAI
                     ASGSAALQPRLERIFLAAGVNVLEGYGLTETSPVISVNCLKKGIRIGTVGPLIDGVQV
                     KIAEDGEILVKGPNVMKGYYNNPEATAEAIDAEGWFHTGDIGEFVEGKFL"
BASE COUNT          557 a          331 c          236 g          583 t
ORIGIN      
        1 tcaagaattt tccttccaca aattcaccga tatcgcctgt atggaaccaa ccttctgcat
       61 caattgcctc ggcagtagct tcaggattgt tgtaatagcc tttcatcacg ttagggcctt
      121 ttactaatat ttcaccatct tcagcaattt tcacttgaac accatcaatc aacggaccaa
      181 cggttccaat acgaatacct tttttcaaac agttaaccga aattacagga gaggtttctg
      241 ttaaaccgta gccttccaat acattcactc cagcagctaa aaatatacgt tccaaacgag
      301 gttgcaaggc agcacttcct gatgcaattg cttttacttg tccacccaaa gcttcttgcc
      361 attttgagaa aattaatttt cgagcaatgc ttaatttaaa atggtaccat gccgattttc
      421 caatcacatc atattcttca cccaattcaa ctgcccagaa aaacaacttg cgtttaatac
      481 ctgttaattc ttctcctttg gctataattt tatcaaatac tttttctaac aaacgtggta
      541 cagctgtaaa tacatctggt ttcacttctt taatattttc tccaattgta tccaacgatt
      601 ctgcaaagta caccgatgca ctcatacgta tatataagta gtgcaacatg cgctcataca
      661 cgtgacatac aggtaaaaat gttaaaacac gagaataacg atcggcagga atacgcgatt
      721 cacacgccaa tgcgtttgat aatatattat tgtgtgttaa cattactcct tttggatttc
      781 ctgtagttcc agaagtataa ataatagtga ctatatcatc gtttttaatc gcatcacttc
      841 tttttacaat ctctgcttca tcaatagaat cagctaattg agctacctct ttccaattag
      901 gagcaccatt aaccacatca aatgtatagg tattttctaa cgatggaaca tttgatttaa
      961 tacgtgaaat tttttgatat aaatcttctg taccaacaaa actcaacttt ataccagcat
     1021 tgttaaaaat atagttgtaa tcattttctg aaatattggg ataaattgga accatgattg
     1081 cacctatttg ttgaatagca atatccataa tattccactc cacacgattg gaagaaacaa
     1141 cagcaatacg atcacccgtt tgaaccccca acgcaattaa acctcgagaa actaatttgg
     1201 tttgttcaac aaattctttg gtggagatag cctcccattt accattgcgt ttggtaacaa
     1261 acatttggtc tgttggataa tcatttaatt gattgtaaac taaatcaaat aatcgagttg
     1321 gtgtcatatt aatgtgtatt aaattcttac ttcgttttat gacgaatata ggtattatta
     1381 tgattgaaat tttaaaaaag gtgaaaaaaa atttaacact tggttaaaat aaaataaata
     1441 ttcatccaga agtggatttt gataattcac aactcggttt tacttatctt actcaaaaaa
     1501 aacagttaca aatagttact tgcaattatt attctttaat tttacgaaaa agtagactag
     1561 taatttttac aatacttaac tcaataatga atatgaaaaa gatttttttg gtgttggtat
     1621 catttgtagt ttgtgctgta aattcatatt ctcaagaccc ttcatttacg cagttttatt
     1681 ccaaccctat ttatttgaat cctgctt
//
LOCUS       DAOQVQ010000513         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_94161_length_1703_cov_3.197209, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000513 DAOQVQ010000000
VERSION     DAOQVQ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94161_length_1703_cov_3.197209"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKN22_11470"
     CDS             <1..282
                     /locus_tag="PKN22_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNS43369.1"
                     /translation="NGFMGQTTDFVKEYIKSNVLIMGENKALGTARYIHGEMGQGTWT
                     FYGGHDPEDYEHKVGDPHTDLNLYPNSPGYRLILNNILFPAAKKKKQKT"
     gene            369..1004
                     /locus_tag="PKN22_11475"
     CDS             369..1004
                     /locus_tag="PKN22_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNS43370.1"
                     /translation="MEKLYSDFLENNKKWAQAQVDRDPTFFEELSKIQNPPLFWIGCA
                     DSRVPANQIIGAQPGDVFVHRNIANMVVHSDMNMLSVLDYAVTVLNVKHVVVCGHYGC
                     GGVATAMSNKHVGIVDNWIAHIKDSYRFHKKELDAIADYQQRYDRFVELNVKEQVFNL
                     AETYIVQKAWSEGIPLHIHGWAYSLKTGLINDLNCTIKNNEMLQEVDRLDI"
     gene            1006..1587
                     /locus_tag="PKN22_11480"
     CDS             1006..1587
                     /locus_tag="PKN22_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HNS43371.1"
                     /translation="MNLVFATHNRNKALEIQALMPKGISIQTLDDIGCHDDIPETAST
                     FEGNALLKAQYVAEKYNVACFSDDSGLEVDFLNGQPGVYSARYAGEAKNDRANLQKVL
                     TELEGQTNRGAQFKTVICLIINQKTHYFEGVVRGIIRHQPAGDGGFGYDPIFEPEGNS
                     ITFAEMSREEKNAQSHRARALAKMIAFLNETQF"
BASE COUNT          579 a          289 c          353 g          482 t
ORIGIN      
        1 aatggtttta tggggcaaac cactgatttt gtaaaagaat acatcaaatc aaacgtgttg
       61 attatgggtg aaaacaaggc actgggtact gcaagataca tacatggaga aatgggacag
      121 ggcacttgga cgttttatgg cgggcacgac ccagaagact atgaacataa ggtcggcgat
      181 ccacataccg atttgaattt atatcccaac tcaccgggct atcgcttgat attaaacaat
      241 attttatttc cagccgccaa aaagaaaaaa caaaaaacgt aggttcattt ctcgttcaaa
      301 acaaattaaa aaagtcgttt ttgattggaa tataaacact atttttggtc gaatataaaa
      361 cacaaacgat ggaaaaatta tacagcgatt ttcttgaaaa taacaaaaaa tgggcgcaag
      421 cacaggtgga tagagaccca actttttttg aagaattatc aaaaattcaa aatccaccgt
      481 tgttttggat tggttgcgcc gattcgcgcg tgcctgctaa ccaaattatt ggagcgcaac
      541 ctggagatgt ttttgtacat agaaatattg ctaacatggt ggtgcattcg gatatgaaca
      601 tgttgagtgt gttggattat gcggttactg ttttaaatgt taaacatgta gtagtttgtg
      661 gccattatgg atgtggtggg gtggctaccg caatgtcaaa caaacacgtt ggaattgtag
      721 ataactggat tgctcacatc aaagattcgt atcgttttca taaaaaagaa ttagacgcca
      781 ttgctgatta ccaacaacgt tatgaccgtt ttgttgagtt aaacgtaaaa gaacaagtat
      841 ttaatcttgc agaaacgtat attgttcaaa aagcatggag tgaaggaatt ccattgcaca
      901 ttcacggctg ggcttactcg ctaaaaacag gattaatcaa cgatttgaat tgcactataa
      961 aaaataacga aatgcttcaa gaagtagatc gtttggatat ttaagatgaa tttagttttt
     1021 gcaacacaca accgtaataa ggcattggaa attcaagcgt taatgcccaa gggaatttcc
     1081 attcaaaccc tagacgatat tggatgccac gatgacattc cagaaactgc ctcaactttt
     1141 gaaggaaatg cacttttgaa agctcaatat gttgccgaaa aatacaatgt ggcttgtttt
     1201 tcggatgatt ctggtttgga agtagatttt ttaaacggac agccaggtgt ttattcggca
     1261 cgctatgcag gtgaagctaa aaatgacagg gctaatctgc aaaaagtatt aaccgaactt
     1321 gaaggtcaaa ccaatcgtgg tgcacaattt aaaacggtta tttgtttgat aatcaatcaa
     1381 aaaacacact attttgaagg agtggtaaga ggcataattc gccatcaacc tgctggcgat
     1441 ggtggttttg gctatgatcc aatttttgaa cccgaaggaa attccatcac ctttgcagaa
     1501 atgtcgcgcg aagagaaaaa tgcacaaagt caccgtgcac gtgcattggc taaaatgatt
     1561 gcttttttaa acgaaacaca attttaaaga attctgtaac cagataatgc ttatttttgc
     1621 aaccaaatgg cacaaacggc aataaatatc aaaaacaaac gcgcaaaatt tgagtaccac
     1681 ttgttggata cttttgttgc ggg
//
LOCUS       DAOQVQ010000514         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_94261_length_1702_cov_3.489375, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000514 DAOQVQ010000000
VERSION     DAOQVQ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94261_length_1702_cov_3.489375"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="thiL"
                     /locus_tag="PKN22_11485"
     CDS             complement(<1..587)
                     /gene="thiL"
                     /locus_tag="PKN22_11485"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNS43372.1"
                     /translation="MSENKSLDQLGEFGLIDHLSSQFTPQNASTIKGIGDDCAVISIS
                     DEEAMVVSTDMLVEGVHFNLMYTPLKHLGYKAVVVNLSDVCAMNAVPEQVTVSIAFSS
                     KFPVEAIDELYKGIKAACDFYNVDLVGGDTSTSYSGLTISITAMGRAKKDKIVYRSGA
                     KDFDLVVVTGDLGAAYMGLQVLEREKEVFKANPSI"
     gene            complement(589..1398)
                     /locus_tag="PKN22_11490"
     CDS             complement(589..1398)
                     /locus_tag="PKN22_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43373.1"
                     /translation="MKFFLKIALFVGLILIGQNLTAQTLQEVKRFPITENSLWTVDAL
                     NNVIVSSRDRLTKVDENGKLLFDQSQKSLGKIDKIDIVNSLKIMGFSEAQQLICFFDN
                     SLTSFDKCIDLSDYDLMSVSKVAISGQSDKIWVLDQVNSTLQLISMKGLLQSQEVKNL
                     NGILSIDQIVQMNEWNNRLFLLDSTKGIYVFDIYGTLTRFIEMPGAKWVQFYNNYLIC
                     LIENELKFISLEDASVNVSLPLNYQNCIDFFANEQYIYFKTPTEIIKTKLN"
     gene            complement(1395..>1702)
                     /locus_tag="PKN22_11495"
     CDS             complement(1395..>1702)
                     /locus_tag="PKN22_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNS43374.1"
                     /translation="VYMNDTTKGAHQWLIEFINHPNDLRNFQRLLDHTLKDLNSDYEA
                     KRYKDLILQEPQLKILPTGAFDVWLKSNNKLGGQNKVPRLANNREIAEQILESNQLQ"
BASE COUNT          532 a          346 c          265 g          559 t
ORIGIN      
        1 tgtatggacg gattggcttt aaaaacttct ttttcgcgct ccaacacttg caagcccatg
       61 taggctgcac ccaaatctcc tgttacaact accaagtcaa aatctttggc accacttctg
      121 taaacaattt tgtctttttt agcacgcccc attgctgtaa tactaatggt taacccagaa
      181 taagaagtgc tggtatcacc accaaccaaa tcaacgttgt aaaaatcgca agctgcttta
      241 attcctttgt ataattcatc aattgcctct acaggaaatt tagacgaaaa cgcaatggaa
      301 acagtaacct gctctggcac tgcattcatt gcacacacgt cagataaatt tacaaccacc
      361 gctttgtaac ccaaatgttt caagggagtg tacattaaat taaaatgaac gccttcaacc
      421 aacatgtcgg tagaaactac cattgcctct tcatccgaaa tggatattac cgcacaatca
      481 tcaccaattc ctttgattgt ggatgcattt tgaggtgtaa actgactgct caaatgatca
      541 attaatccga attcacctaa ttggtcaagg cttttatttt ctgacatgtt aatttaattt
      601 tgttttaata atttctgttg gtgttttgaa ataaatgtat tgttcgtttg caaaaaaatc
      661 aatacaattt tgataattca acggcaaaga tacgttaact gatgcatctt ccaatgaaat
      721 aaattttaat tcattttcta ttaggcaaat tagatagtta ttgtaaaact gcacccactt
      781 tgctccaggc atttcaataa aacgtgttaa cgtgccataa atatcaaaca cataaatgcc
      841 tttggtgcta tctaacagaa acaaacgatt gttccattca ttcatttgca caatttgatc
      901 gatggaaaga atgccattca aattttttac ttcttgactt tgcaacaatc ccttcatgga
      961 aatcaattgc aaggtggaat taacttgatc gagcacccaa attttatccg attgaccgga
     1021 aatggccact ttgcttacac tcatcaagtc ataatcgctt aaatcaatgc atttatcaaa
     1081 cgaagtgagc gaattatcaa aaaaacaaat taactgttgt gcctcagaaa aacccattat
     1141 tttcaacgaa ttgacaatgt ctattttatc aattttacct agcgatttct gactttggtc
     1201 aaacaatagt tttccgtttt catccacttt tgtcagccga tcacgcgatg aaacaataac
     1261 attattcaat gcatcaacgg tccacagtga attttcagta attggaaaac gcttcacttc
     1321 ttgtaaggtt tgtgcagtaa ggttctgacc aatcaaaatg agtccaacaa aaagtgctat
     1381 tttcaaaaag aatttcattg caattggttg ctttccaaaa tttgttccgc aatttcacgg
     1441 ttatttgcca aacgaggcac tttgttttgt ccgcccaatt tattgtttga tttcaaccac
     1501 acatcaaaag ctcctgtggg caatattttt aattgtggtt cttgcaaaat tagatctttg
     1561 tagcgttttg cttcgtaatc cgagttcaaa tctttgaggg tatgatccaa caatcgttga
     1621 aaatttcgca aatcgttggg atggtttatg aattcaatca accattggtg cgctcctttt
     1681 gtcgtatcgt tcatatacac ag
//
LOCUS       DAOQVQ010000515         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_94360_length_1701_cov_4.136087, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000515 DAOQVQ010000000
VERSION     DAOQVQ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94360_length_1701_cov_4.136087"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="asnB"
                     /locus_tag="PKN22_11500"
     CDS             complement(<1..1040)
                     /gene="asnB"
                     /locus_tag="PKN22_11500"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNS43375.1"
                     /translation="MCGIAGFIDFQKNSSYETLQKMTDTLYHRGPDGAGYELFQTENA
                     TIGFGHRRLAIIDLTEHGKQPMQLNNLWITFNGEIYNYQEIKAELVELGHTFVSDSDT
                     EVILHAYQQWGDACLQKMIGMFAFVIFDNNKNEVFLARDRTGVKPLHYYFKDGLLLFA
                     SELKAFHQHPNFHKNINPHAVASFMQYGNVPSDACIFEDCSKLLPGHFAVVKLHYRNM
                     SISNYWNVYDFYNQPKLQLSFDEAKLKTEELITSACNYRMIADVPVGIFLSGGYDSTT
                     VTAILQKDRTQKLKTYTIGVPDIGLNEAPYAADIAKHLGTEHTTYTCTQKEALDVITE
                     LPFYYDEPFADS"
     gene            1140..>1701
                     /locus_tag="PKN22_11505"
     CDS             1140..>1701
                     /locus_tag="PKN22_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS43376.1"
                     /translation="MKKIKDAINIVKQLTFSRLWNMVVLYASFIYSRWKKKDFHRGMP
                     FSLSIEPTTACNLGCPECPSGLKQFTRPTGRLKLATHQEMLQQLSKHVFYINYYFQGE
                     PFLNPQFLDLIKEAKKHKIYTATSTNAHFINNKKVAQDIVKSGLDRLIISIDGLTQQT
                     YESYRVHGKLTKVIAGAKWMIEAKKEL"
BASE COUNT          558 a          328 c          302 g          513 t
ORIGIN      
        1 ctgctatcgg caaatggttc gtcgtaataa aaaggtaatt cagtaataac atccaacgcc
       61 tctttttggg tgcacgtata agtggtgtgt tctgttccca aatgcttggc aatgtctgct
      121 gcataaggcg cttcattcaa gccaatatcg ggaacaccta tggtatatgt ttttaatttt
      181 tgtgtgcgat ctttttgtaa tatagccgta acggttgtgc tgtcatagcc gccacttaaa
      241 aaaattccta caggcacatc ggcaatcatt cgatagttgc atgcagaggt aattaattct
      301 tcggttttca acttcgcttc gtcaaaagaa agctgaagtt taggctgatt gtaaaaatca
      361 tacacattcc aatagttcga tatcgacata tttcgatagt gcaattttac caccgcaaaa
      421 tgacccggca ataacttaga acaatcctca aaaatacacg catcactcgg aacattgcca
      481 tattgcataa acgaagcaac tgcatgcgga ttgatgtttt tatgaaaatt aggatgctga
      541 tgaaatgcct tcaattccga tgcaaacaac aacaaaccgt ctttgaaata ataatgcaat
      601 ggtttcaccc ccgttctgtc acgcgctaga aacacctcgt ttttattgtt atcgaaaatc
      661 acaaaggcaa acatgccaat cattttttgc aaacatgcat ctccccattg ttggtaggcg
      721 tggagaataa cctctgtgtc cgaatcactc acaaaagtgt gacctaattc cactaattcg
      781 gctttaattt cttggtaatt gtaaatttca ccgttaaaag ttatccaaag attgttcaac
      841 tgcatgggtt gcttgccgtg ctccgttaaa tcaatgattg ctaaacgacg gtgaccaaaa
      901 ccaatcgtag cattttccgt ttgaaataat tcatatcccg caccatctgg tcctcggtga
      961 tacaacgtat cggtcatctt ttgaagcgtt tcataagatg agtttttttg gaagtcaata
     1021 aatcctgcaa ttccacacat gaccacaaag ataataatcg cttaaaaata ggaaagatat
     1081 gcgtaaaatg tttttgtgta ttgcaaagaa tagttcaaaa taaacgctaa atttgaaaag
     1141 tgaaaaagat aaaagatgcc ataaatattg tgaaacaact gacttttagt aggttgtgga
     1201 atatggtagt attgtatgct tcgtttattt attcacgttg gaaaaagaaa gattttcacc
     1261 gtggtatgcc tttttcattg agcattgaac caacaactgc atgtaattta ggttgccccg
     1321 aatgtcccag tggactcaaa caatttactc gtccaactgg tcgcctaaaa ttagctacac
     1381 accaagaaat gttgcagcaa ttatccaaac atgtttttta tatcaattat tattttcaag
     1441 gagaaccgtt tttaaatcct caatttttag acttaattaa agaggcaaaa aaacacaaaa
     1501 tttatacagc aacatccacc aatgcccatt tcattaataa caaaaaagtg gcgcaagata
     1561 tcgttaaatc tggattggat cgtttgatta tttccattga cggactaacg caacaaacct
     1621 atgaaagcta ccgagttcat ggcaaattaa ccaaagttat tgcgggtgca aaatggatga
     1681 ttgaagcaaa aaaagagttg a
//
LOCUS       DAOQVQ010000516         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_95341_length_1691_cov_1.994499, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000516 DAOQVQ010000000
VERSION     DAOQVQ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95341_length_1691_cov_1.994499"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PKN22_11510"
     CDS             complement(<1..949)
                     /locus_tag="PKN22_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNS43377.1"
                     /translation="MAIEQSPATSQTDNLRDLLNSNQYEAVTYTDGPSLIVAGAGSGK
                     TRVLTYKIAYLLQNGWHPSQILALTFTNKAAEEMKGRIAKLVGEQRAKYLWMGTFHSI
                     FSKILRYEASHLGFTSNFTIYDSSDSKSLIRSLIKELQLDDKVYKVNNIQSRISFAKN
                     QLVTPATYANDSDMMRSDMNSKTPRFKEVYQLYCQRCRNSNAMDFDDLLLYTNTLFEK
                     HPEILKTYQERFGYILVDEYQDTNRAQHLIVSKLAANHQRICVVGDDAQSIYSFRGAN
                     IDNMLMFQRQYEGCKLFKLEQNYRSTQTLVNAANSLISRN"
     gene            1108..1683
                     /locus_tag="PKN22_11515"
     CDS             1108..1683
                     /locus_tag="PKN22_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNS43378.1"
                     /translation="MKSIKGTQTEKNLMHSFAGESQARMRYTYFASAAKKEGFEQIAA
                     IFTETADQEKEHAKRMFKFLEGGMVEITASFPAGVIGRTIDNLQAAAAGEHEEWSHDY
                     PHFADVAEQEGFLSIAAMYRNIAIAEKAHEERYRAFVANIETASVFAKEDEVVWQCRN
                     CGYIHVGKEAPQACPACLHPQAYFEVKKENM"
BASE COUNT          506 a          299 c          368 g          518 t
ORIGIN      
        1 tattcctgct gattaatgag ttggcggcgt taacgagcgt ttgcgtagaa cggtaatttt
       61 gttctaattt gaatagttta cagccttcat attgccgttg aaacatcaac atattgtcga
      121 tattggcgcc acgaaacgaa tagatgcttt gtgcatcgtc gcctaccacg caaatacgtt
      181 gatgattggc tgccaattta ctcacaatta aatgctgcgc acggtttgta tcttgatact
      241 cgtccaccaa aatgtaacca aatcgttctt gataggtttt aagaatttca ggatgttttt
      301 caaaaagggt attcgtgtaa agtaacaaat catcaaaatc cattgcgttc gagttcctgc
      361 agcgctggca atagagttgg taaacttctt taaaacgtgg cgttttggaa ttcatgtcgg
      421 agcgcatcat gtccgaatca ttggcataag tggctggcgt taccaattga tttttggcaa
      481 atgaaatgcg actttgtatg ttgtttactt tataaacttt gtcgtcgagt tgtaactctt
      541 tgattaaact acgaatcaaa cttttggaat cgctcgaatc gtatatcgtg aaattggagg
      601 taaacccaag atgcgaggct tcgtagcgta gaatttttga aaaaatggag tgaaaggtcc
      661 ccatccagag atatttggcg cgttgttcac ctaccaattt ggcgatacgt cctttcatct
      721 cttcggcagc tttattggtg aatgtcaacg cgagaatttg gcttggatgc cagccgtttt
      781 gtaataaata ggcaatttta taggtcaata cgcgtgtttt accagaacca gctccagcca
      841 caataagcga agggccgtcg gtataagtga cagcctcgta ttggtttgag ttaagtaaat
      901 ctcttaaatt atcggtttga gaggtagctg gtgattgctc gatagccata gcgttcttga
      961 aagattacaa actacaaagg tacaaaaagc attcaaactt tacaatggaa aattagtact
     1021 cagaaaaata agttggtgta tggttatttc aaaataaaac gctaatttta cactccgata
     1081 gaactactaa ttttaaattt ataatctatg aaaagtatta aaggaacaca aactgaaaag
     1141 aatctaatgc attcttttgc tggtgaaagt caggctcgta tgcgttacac ctattttgca
     1201 agtgctgcta aaaaggaagg ttttgaacag attgctgcta tttttacgga aacagccgat
     1261 caagaaaaag agcatgccaa acgcatgttt aagtttttag aaggtggtat ggtagaaatt
     1321 actgcaagct tccctgctgg tgttattggt cgtactatcg ataatctcca agctgctgca
     1381 gccggtgaac acgaagagtg gtcgcacgat tatcctcatt ttgcggatgt ggcagagcaa
     1441 gaaggtttct tatcaattgc ggctatgtat cgtaatatag cgattgcaga aaaagcacac
     1501 gaagagcgtt atcgtgcatt tgtggcaaat atcgaaacag cttctgtttt tgctaaagaa
     1561 gatgaagttg tttggcaatg tcgcaattgt ggctacattc acgtaggcaa agaagctcct
     1621 caagcttgtc ctgcttgctt gcatcctcaa gcttattttg aagttaagaa agaaaatatg
     1681 taatagcaaa a
//
LOCUS       DAOQVQ010000517         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_95572_length_1688_cov_3.192284, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000517 DAOQVQ010000000
VERSION     DAOQVQ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95572_length_1688_cov_3.192284"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PKN22_11520"
     CDS             <1..407
                     /locus_tag="PKN22_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43379.1"
                     /translation="ELIDQFIMGPICVGISFLEATLVHNIFSFAQNPLYVPDTHLTFH
                     LGMDVLVPRKNNPFYWHNRNAFFNSISPQLKPHFELKKFPYGALPLPKRAVKWMLNPS
                     LFTRNYLNLEGKNFAQRLKTRLDEIRWRLLQK"
     gene            complement(409..1554)
                     /locus_tag="PKN22_11525"
     CDS             complement(409..1554)
                     /locus_tag="PKN22_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNS43380.1"
                     /translation="MKSVYLDNAATTPMAKEVIEVMTNAMYNNFGNPSSTHAFGRSTK
                     ALLENARRSIAKLLNCEPSEIIFTSCGTEADNMAIDSAVELLGAKRIITTPIEHHAVE
                     HTIDALVKAGKVELVHVNMNSEGIVDLAHLEQLLADTSKKTVVSLMHANNEIGTLLPL
                     EKVNELCKKYGAIFHSDTVQTMGHYAFDLKKLDIDYITCSAHKFHGPKGVGFLYVNKK
                     AKVQNLIQGGSQERGLRGGTENIYGIIGLAKALELAYTDLEAHQQHVQQIKSHMIKRL
                     KNELPGVEFHGTIEPEKSLYTVLNVKLPATEKGSMLLFTLDLKGVACSGGSACTAGAT
                     TGSHVLRGIKADTSRPNARFSFSRYTTIEEVDYAVDVLVEVYQNVLV"
BASE COUNT          483 a          328 c          304 g          573 t
ORIGIN      
        1 aagaactcat cgatcaattc atcatgggac caatatgtgt gggaatttct tttttagaag
       61 ctacattggt acacaatatt ttttcgtttg cacaaaatcc actgtatgtt ccagatacgc
      121 atttaacgtt tcatttgggg atggatgtgt tggtgccgcg caaaaacaat ccgttttatt
      181 ggcacaatcg aaatgcgttt tttaattcaa tatccccaca attaaaacct cattttgagc
      241 ttaaaaagtt tccttatggg gcattgcctt tgcctaaaag agctgtaaaa tggatgctta
      301 atccaagttt gtttactcga aattatttga atttggaagg aaaaaacttt gcacagcgac
      361 taaaaacccg tttggatgaa atcagatggc gcttgttgca aaaatagatt acaccaaaac
      421 gttttgatac acttctacca aaacatcaac agcataatca acctcttcga tggtggtata
      481 gcgtgaaaat gaaaaacgag catttggtcg cgaagtatct gctttaattc ctctcaatac
      541 gtgtgaacct gttgttgctc ctgccgtaca agcacttccg ccactgcatg ccacaccttt
      601 taaatcaagg gtgaataaca acatactgcc tttttcggtt gctggcaatt taacgtttaa
      661 aacggtgtat aatgattttt caggttcaat tgttccgtga aattcaacgc caggcaattc
      721 attttttaga cgcttgatca tgtgcgattt tatttgctgt acgtgttgtt gatgtgcctc
      781 taaatctgta tatgccaatt ccaacgcctt tgctaatcca atgataccgt aaatattttc
      841 agttccacct cttaacccgc gctcttgact tcctccttgg atgaggtttt gcacttttgc
      901 ttttttgttc acgtataaaa atccaactcc ttttggtccg tgaaatttat gcgccgaaca
      961 ggtaatataa tcaatgtcta attttttcaa atcaaatgca tagtgcccca ttgtttgcac
     1021 cgtgtctgaa tgaaaaatag caccgtattt tttacaaagt tcgttgactt tttccaacgg
     1081 gagcaacgtg ccaatttcat tgttggcatg catcaacgaa accactgttt ttttagaagt
     1141 atcggctaat agttgttcca aatgcgctaa atcaacaatt ccttcgctgt tcatgtttac
     1201 atgtactaat tccacttttc ctgctttcac caaagcatca atggtgtgtt caactgcgtg
     1261 gtgttcaatt ggagttgtga taatgcgttt tgcaccaagt aattcaactg ccgaatcgat
     1321 ggccatgttg tcggcttctg ttccacagct tgtaaaaatg atttcagaag gctcgcaatt
     1381 caataattta gcaatagaac gacgtgcgtt ttctaataat gcttttgttg aacgtccaaa
     1441 agcatgtgtt gatgaaggat ttccaaaatt gttgtacatc gcattggtca tcacttcaat
     1501 tacttccttt gccattggag tggtggctgc attatctaga taaacgcttt tcattgttga
     1561 aaattagaat tgagggtaca aatatacagt cttcggaaat gaaaaaaaat gaatttgaga
     1621 aatatctaac acatttctta aatttctttg tgctgattct gtcaaagttg taataaaata
     1681 tgcatttt
//
LOCUS       DAOQVQ010000518         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_95822_length_1686_cov_2.414470, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000518 DAOQVQ010000000
VERSION     DAOQVQ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95822_length_1686_cov_2.414470"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKN22_11530"
     CDS             <1..207
                     /locus_tag="PKN22_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43381.1"
                     /translation="KKFIVKRVVKKFSKIDKGTYDFLFSKTMQSMSNGNPFASPINVK
                     TNLQGGCLGVWAGYSPILDTLICD"
     gene            322..>731
                     /locus_tag="PKN22_11535"
     CDS             322..>731
                     /locus_tag="PKN22_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HNS43382.1"
                     /translation="MKKRISLVFSVSCGLFLSHLTFSQDSLKTKKEDNSLNEVVIQGN
                     RIQIPFSQQNRDIVVVDSKVIETLPVKSLNELLGYVAGVDVRQRSPWGGQTDVSINGG
                     SFDQTLVLLNGLKIIDPQTGHNMMNLPISPDAIER"
     assembly_gap    732..741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            752..>1686
                     /locus_tag="PKN22_11540"
     CDS             752..>1686
                     /locus_tag="PKN22_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS43383.1"
                     /translation="MKGAAASAYGINALNGVINIITKQPDASGFILNVNSGSSFEKDT
                     SKNKLYGSLGVDVTASLAFKKSQHLLSLSTTQSNGYRYNTPMNNNKAYYQNNINIGKD
                     HHLQVMGGFIYNDFGANGFYAAPFDVNSREIVQTGIAAIKGDFKALKIWTIRPSVSYR
                     YNHDDYIFIRDNPKYYENNHFTNVIDASINNSIQTSIGTVGVGVEYRGEIINSNNLGQ
                     WTRDNYGLYAEFSFTRVKNFTANVGAYLNYSKFYNFKLLPSLDLGYQVHKNWRLFANV
                     GSGNRIPTYTDWYYVSKQNIGNSKLKPENALHAEGG"
BASE COUNT          550 a          307 c          303 g          516 t
ORIGIN      
        1 aaaaaattca tcgtcaaacg cgttgttaaa aaattttcaa aaatagacaa aggcacctac
       61 gatttcttgt tttcaaaaac aatgcaatcc atgtccaatg gtaatccatt tgcctcccct
      121 attaacgtga aaaccaacct tcaagggggc tgtttgggag tatgggcggg ttattcacct
      181 attttggaca ctttaatctg cgattaaaca cttgatttat tgcgaattac aaatcatctg
      241 tttaaaattt aacaaaaaaa acttcatgtt ttggttatgc tttttagaat ctttctaaat
      301 tcggccccaa aatttatcaa tatgaagaaa agaatttctc tagtattttc tgtctcttgt
      361 ggcttatttt tatctcactt aacattctca caagactcat taaaaaccaa aaaagaagac
      421 aactcactta acgaagtagt tattcaagga aacagaattc agattccatt ttcacaacaa
      481 aacagagaca tcgttgtagt cgattctaaa gtaatcgaaa cattgcctgt aaaatcttta
      541 aatgaacttt tgggctacgt tgccggagtg gatgtgcgtc aaagaagtcc gtggggtgga
      601 caaacagacg taagcattaa cggtggttcg tttgaccaaa cgttggtttt attgaatggt
      661 ttaaaaatca tcgacccaca aacggggcac aatatgatga acctcccgat tagtccggat
      721 gccattgaac gnnnnnnnnn ncatcgaaat tatgaaaggc gcagctgcaa gtgcttatgg
      781 tattaatgcc ttgaatggtg ttatcaacat cattaccaaa caaccagatg cttctggatt
      841 tattttaaac gttaactcag gtagttcttt tgaaaaagac acttctaaaa acaaacttta
      901 tggttcttta ggtgttgacg ttactgctag tttagcgttt aaaaaatcgc aacatttatt
      961 gtcattgagc actacacaaa gtaatggtta ccgttacaac acaccaatga acaacaataa
     1021 agcgtattac caaaacaaca tcaacattgg taaagaccac catttgcaag taatgggtgg
     1081 atttatttac aacgattttg gtgccaatgg tttttatgct gctccttttg atgtcaattc
     1141 aagagaaatt gttcaaacgg gtattgcagc tatcaaaggt gatttcaaag cgttgaaaat
     1201 ttggaccatt cgtccatccg ttagctaccg ttataaccac gatgattaca tctttattcg
     1261 cgacaatcct aagtattacg aaaacaacca ctttacaaat gtgattgatg cttctatcaa
     1321 caacagtatc caaacatcta ttggtacggt tggtgttggt gttgaatacc gtggagaaat
     1381 cattaacagt aacaacttgg ggcaatggac tcgtgacaat tatggtttgt atgctgagtt
     1441 ttcatttacg cgagttaaaa actttactgc caatgtaggt gcttacttga attacagtaa
     1501 attttacaac ttcaagttgc ttccatcgtt ggatttaggt taccaagtac acaaaaactg
     1561 gcgtttattt gcaaatgttg gctctggaaa ccgcattcct acctatacag attggtatta
     1621 cgtaagtaaa caaaacattg gtaacagcaa attgaaacca gaaaatgcgt tgcatgcaga
     1681 aggtgg
//
LOCUS       DAOQVQ010000519         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_96806_length_1675_cov_4.296914, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000519 DAOQVQ010000000
VERSION     DAOQVQ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96806_length_1675_cov_4.296914"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1268
                     /locus_tag="PKN22_11545"
     CDS             315..1268
                     /locus_tag="PKN22_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNS43384.1"
                     /translation="MKNRITTLFNIQYPIIQAGMIWCSGWRLAAAVSNAGGLGIIGSG
                     SMYPEVLEEHLQKMKEATDKPFAVNLPMLYPNIDKHIELIIKYQVPIVFTSAGNPKTW
                     TSHLKSHGIKVVHVVSSLKFALKSQEAGVDAVVAEGFEAGGHNGREETTTLCLIPAIA
                     KELQIPLIAAGGIGTGRAMLAAMNLGADGVQIGSRFIATPESSAHENFKQAVIATKEG
                     ETSLTLKELTPVRLIKNQFYQQVEQAYEKCATVEELQTLLGRGRAKKGMFEGDLVEGE
                     LEVGQVSGMIHEIKPAAAIVQEILQEFKEAIAEQTTSKYDF"
     gene            1268..>1675
                     /locus_tag="PKN22_11550"
     CDS             1268..>1675
                     /locus_tag="PKN22_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNS43385.1"
                     /translation="MIHFERFTLKNGLIVLFHKDTTTPMAVVNTLYDVGARDEEESKT
                     GFAHLFEHLMFGGSINIADFDTPLQQAGGENNAFTSNDITNYYDVVPAQNLETALWLE
                     SDRMLSLAFTDKSLEVQRNVVIEEFKQRYLNQPY"
BASE COUNT          588 a          250 c          339 g          498 t
ORIGIN      
        1 tcaatagata gtgctgaata agctaaattg ctaagtatca gtagtaaaaa aactattaaa
       61 acttttaatt ttttcatttt taaaaagtat aaacacaaat agtgatgtga ataattctaa
      121 tatattagag tacaatatta gtttcatttt ttgaaaaatc aaagaaatag acaaattatt
      181 tacgtttttt gagaaaaata aataacactg attgttggtt cgatattgtg ttgaaatcaa
      241 attttaacag atagggtaga agttctttcc agttatttaa aaaataatta acttcgtaat
      301 tcatcaaatt gaatatgaaa aatagaataa caacattatt taatatacaa tacccaatta
      361 ttcaagcagg catgatttgg tgctcgggtt ggcgtttggc agcagctgtt tcaaatgcag
      421 gaggtttagg catcattggt tctggttcta tgtacccaga agttttggaa gagcacttgc
      481 aaaaaatgaa agaagcaacc gacaaacctt ttgcggtgaa tttgccaatg ctttatccca
      541 acattgataa acacattgaa ttaattatta aataccaagt gccaattgtt tttacatcgg
      601 caggaaatcc aaaaacgtgg acttctcacc taaaaagcca tggaattaag gtagtgcacg
      661 ttgtttcaag tcttaaattt gcactaaaat cacaagaagc aggtgtggat gctgtagtgg
      721 cagaaggttt tgaagcaggc ggacacaatg gacgggagga aacaactacg ctttgtttga
      781 ttcctgcaat tgcaaaagaa cttcaaattc cacttattgc tgcgggaggc attggtactg
      841 gtagagctat gttggcagcc atgaatttag gtgccgatgg cgtgcaaatt ggaagtcgct
      901 ttattgctac gcctgaatca agtgcacacg agaattttaa acaagcagta attgcaacaa
      961 aagaaggtga aacctctcta acgttgaaag aattaacacc tgttcgactc atcaaaaatc
     1021 aattttatca gcaggttgaa caagcctatg aaaagtgtgc aacagttgaa gaattgcaaa
     1081 cattgcttgg tagaggaaga gctaaaaaag gaatgtttga gggcgattta gtagagggag
     1141 aattggaggt tggacaagta tctggcatga ttcatgaaat aaaaccagct gctgctattg
     1201 tgcaagaaat tttacaagaa tttaaagaag caattgctga acaaacaaca tcaaaatacg
     1261 atttttaatg atacactttg agcgctttac tttaaaaaat ggactaattg ttctttttca
     1321 taaagacact acaactccaa tggcagtggt taatacgtta tatgatgttg gggcaagaga
     1381 tgaagaagaa tcaaaaacag gttttgcaca tttatttgag cacctaatgt ttggtggttc
     1441 tatcaatatt gctgattttg acacgccttt gcaacaagcg ggtggcgaaa acaatgcttt
     1501 tacctccaac gatattacca attattacga tgtggtgcca gcgcaaaatt tggaaacagc
     1561 tttgtggtta gaaagcgata gaatgttgtc attggcgttt actgataaaa gcttggaagt
     1621 gcaacgaaat gttgtgattg aagaatttaa acaacgctat ttgaatcaac cgtat
//
LOCUS       DAOQVQ010000520         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_96914_length_1674_cov_4.064237, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000520 DAOQVQ010000000
VERSION     DAOQVQ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96914_length_1674_cov_4.064237"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PKN22_11555"
     CDS             <1..1167
                     /locus_tag="PKN22_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43386.1"
                     /translation="NPSQMMREGDKLGWEAYQSHRDALMQWIGGFAQAEFSKGRWNAF
                     ANVSFVANSYKGIDYFKKKELHVGDTIIYIGAKDSSIVYDGKIYSHSSKELKYNQTKT
                     VWRTGFTVKAGTNFNINEKNNVFLNLGYLDRTPLFSNVVDNNTNEVFSTIVNEKVYAL
                     EAGYGFRSKKVSINANGYFTYWKNKPFPFGVNVPDPNDPGTFVRANVNGMDALHMGIE
                     LDVAYRIIKSLTLEVMGSYGDWTWQSAETIDVLGTKFTFDAKGVHVGNSAQSTYAASL
                     RWEFVKNAYITAKYTFFDRYYSDFDPFSLTGANGGKESWKIPSYGLLSAHAGYTLKFK
                     KSALAFRANVFNILNSLYISDAKNNQNGSGFDAASAGVFFGQGTTFNVSIGFEF"
     gene            1180..>1674
                     /locus_tag="PKN22_11560"
     CDS             1180..>1674
                     /locus_tag="PKN22_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43387.1"
                     /translation="MKKLVLTLSLFATTSLFAQVISIADARNAGVGQTVTVKGVVTNG
                     SELGNIRYLQDETGGIAAFGGSISGINRYDSITVTGPLTEFNGLLEIGTGQSGGNPTY
                     TNHGAAVVIPQPLVVPISAVNESIEGQLITISNVTFTTTGSFASGNSTVQITNGSQTF
                     DVRIN"
BASE COUNT          544 a          274 c          331 g          525 t
ORIGIN      
        1 aacccttctc aaatgatgcg ggaaggtgac aaactaggtt gggaagccta tcaaagtcac
       61 cgtgatgctt tgatgcaatg gataggtggt tttgcacaag ctgaattttc aaaaggccgt
      121 tggaatgctt ttgctaatgt atcttttgtt gcaaacagct ataaaggaat tgattatttt
      181 aaaaagaaag aattgcatgt aggcgacact atcatttaca ttggtgcaaa agattcatct
      241 attgtttatg acggaaaaat ttacagccat tcatctaaag agttgaaata caaccaaaca
      301 aaaacagttt ggagaacagg ttttacagta aaagcaggaa ccaatttcaa tattaatgag
      361 aaaaacaatg tgtttttaaa ccttggttat ttagacagaa caccgttgtt tagcaatgtt
      421 gttgataaca ataccaacga agtttttagt acaattgtca acgaaaaagt atatgcactt
      481 gaagctggat atggttttag aagcaaaaaa gtgtctatta atgccaatgg ttattttact
      541 tattggaaaa acaaaccttt tccttttggt gtaaacgtac cagatccaaa cgacccaggg
      601 acttttgttc gtgctaacgt aaatgggatg gatgctttgc acatgggtat tgaattggat
      661 gttgcatacc gcatcattaa aagcttaaca ttagaagtaa tgggttctta tggtgattgg
      721 acgtggcagt cggcagaaac tattgatgtg ttgggaacaa aattcacttt tgatgccaaa
      781 ggtgttcacg ttggtaactc agcacaatcc acttatgcgg catccttacg ttgggaattt
      841 gtaaaaaatg cttacatcac agctaaatac accttcttcg atagatatta ttctgacttt
      901 gatccattta gtttaactgg agcaaatggc ggaaaagaat catggaaaat acctagttat
      961 ggcttgcttt cagctcacgc aggttatacg cttaaattta aaaaatcagc attggctttt
     1021 agagcaaatg tatttaatat attaaacagc ttgtatatct ctgacgctaa aaacaaccaa
     1081 aatggaagtg gttttgacgc tgcttctgca ggtgttttct ttggtcaagg aactacattt
     1141 aacgtatcaa ttggatttga attttaatta aaacacaaaa tgaaaaaatt agtattaaca
     1201 cttagtttgt ttgctacaac atctcttttt gcacaagtca tttctattgc cgatgcaaga
     1261 aatgcaggtg ttggacaaac agttaccgtg aaaggtgttg taaccaacgg tagtgaattg
     1321 ggtaacatcc gttacttaca agatgaaaca ggtggtattg cagcttttgg tggaagtatt
     1381 agtggtatca accgttatga ttctatcact gtaacaggac ctctaactga attcaacggt
     1441 ttgttagaaa ttggaacagg tcaatctgga ggaaacccaa cttataccaa tcatggagcg
     1501 gctgttgtca tcccacagcc cttagttgtt cctatttctg cagttaacga atccattgaa
     1561 ggtcaattaa ttaccatttc aaatgttaca tttactacaa ctggtagctt tgcaagtggt
     1621 aattcaactg ttcaaataac aaacggttca caaacttttg atgtgcgtat taat
//
LOCUS       DAOQVQ010000521         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_98086_length_1662_cov_3.438083, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000521 DAOQVQ010000000
VERSION     DAOQVQ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98086_length_1662_cov_3.438083"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..949
                     /locus_tag="PKN22_11565"
     CDS             179..949
                     /locus_tag="PKN22_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43388.1"
                     /translation="MKGKILIALFFLTVFSNGWTQEVENREKAPSFFGFQIKPIFPAV
                     FMGTTKMNNNVGNFYTSMDMAVSYSFGGVVRAGITKLIAIETGINLTQRNYKVNSSVP
                     DSNLYVQSRLRYVTYDIPVSALFYIRLAKPVYMNVSIGAALTYNPTVAGIKNMPGGYH
                     EFYQIALGKKITGDFLGNMGFEYRTEKAGVFYLGAEARVPFGPLFYLKSTYKNQGHEL
                     TTDAENQGKVNGSYLALQIKYFFPIKKIKGSPVKRPIE"
     gene            949..1416
                     /locus_tag="PKN22_11570"
     CDS             949..1416
                     /locus_tag="PKN22_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS43389.1"
                     /translation="MEIQQIIEQLNMQPHPEGGYFVETYKSVVNVENSINSIATVIYF
                     LLPSGEKSTWHRIKNDEFWFYHSGSPIIVHTIENGAYAETIVGNSVLAKQQPQFLVKG
                     GVIFGASVLEENSYSLVSCVVVPGFEFKDFELFSQAELVAQFPTLADKIALLH"
     gene            complement(1410..>1662)
                     /locus_tag="PKN22_11575"
     CDS             complement(1410..>1662)
                     /locus_tag="PKN22_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43390.1"
                     /translation="KINLVNKLEIGVTPNPATNSLTIQSDFPITGYSILDLQGRIVQS
                     EATTISTNIDIQHLKNGSYLLYLKSNQQQFIVRFEKINQ"
BASE COUNT          523 a          266 c          329 g          544 t
ORIGIN      
        1 tttaccttaa ttatggctgg aataagtttg tttttattcc gcaaacaccc tcaattaaag
       61 tagtttaaaa ctgaatttga agcaatcaaa gctacgtttt agtcaataac tctacttttt
      121 acaaaacaaa tgcgctattt ttccgttttt taggccaaaa aagagcatat ttgtaccgat
      181 gaaaggaaaa attctaatag cattattttt tctaaccgtg ttttcaaacg gttggacaca
      241 ggaggtggaa aatagagaaa aagcaccttc tttttttggt tttcaaataa aacctatttt
      301 tccagcggta tttatgggaa caaccaaaat gaataataat gtggggaatt tttacacatc
      361 aatggacatg gccgttagct actcatttgg tggtgtggtg cgtgcaggaa ttaccaaatt
      421 gattgctatc gaaacgggaa tcaaccttac gcagcgcaat tacaaagtaa actcatcagt
      481 gcctgattca aatttatatg tgcaaagtcg cttgcgctat gttacctacg atattccagt
      541 gtcggcattg ttttatattc gtttggcaaa acctgtttat atgaacgttt ctattggtgc
      601 cgctttgacg tataacccaa cagtggctgg aattaaaaat atgccaggtg gataccacga
      661 attttatcaa attgctttgg gaaagaaaat tacaggtgat tttttgggga atatgggatt
      721 tgaatacaga acagaaaagg ccggcgtttt ttatttagga gcagaagcac gtgttccatt
      781 tggtccgttg ttttatctga aatcaactta caaaaaccaa ggacatgaat tgacaaccga
      841 tgcagaaaat caaggaaagg taaatggttc gtatttggca ttgcaaatta aatatttctt
      901 cccaattaag aaaattaaag gtagccctgt aaaacgccca attgaataat ggaaattcaa
      961 caaattattg agcaattaaa catgcaaccc catcctgagg gtggctattt tgtagaaacc
     1021 tataaatctg tggtgaacgt agaaaattct attaattcca ttgcaacggt catttatttt
     1081 ttattgcctt cgggtgagaa atctacgtgg caccgtatta aaaacgatga attttggttt
     1141 tatcacagtg gaagtcctat tatagtgcat acaattgaaa acggagcgta tgccgaaaca
     1201 atagtaggaa actctgtttt agcgaaacaa caaccgcaat ttttagtaaa gggcggcgta
     1261 atttttggcg catcggtgtt agaagaaaat tcgtattctt tggtttcttg tgtggtggtg
     1321 cctggttttg aattcaaaga ttttgaattg ttttcgcaag ccgaactagt tgctcaattt
     1381 cctacattag ccgataaaat tgcattgctt cattgattga ttttctcaaa acgaacaata
     1441 aattgttgtt gattactttt taaataaagc aaataacttc cgtttttaag gtgttgaata
     1501 tcaatattag tggagatggt tgttgcctcc gattgcacga ttcttccttg caaatctagg
     1561 atggaatatc ccgtaatggg aaagtctgac tgaattgtca acgaattggt tgcaggattg
     1621 ggagttacgc caatttctaa tttattgaca aggttaattt tt
//
LOCUS       DAOQVQ010000522         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_98585_length_1657_cov_3.656679, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000522 DAOQVQ010000000
VERSION     DAOQVQ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98585_length_1657_cov_3.656679"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PKN22_11580"
     CDS             complement(<1..1394)
                     /locus_tag="PKN22_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNS43391.1"
                     /translation="MKKQVINKTKTALKWQLTFFTVVALFILSMNTSCKKTTLLSKGN
                     LAFSRDTVVFDTVFTTIGSTTKQLKIYNKENKPVLISDVELMGGNSSPFRINLDGLQG
                     IKFSEIELPAKDSLFMFVEVTLNPNGGTLPMVIEDSIRFRTNGKDQYIQLAAWGQDIY
                     YHYSDMSGATNNLDLNQGVWPNDKPHLIYGAAFVDEGKQLTIQAGTKIYLHKKSFLFV
                     YKGTLNIQGQLDNEVVIQGDRLESDYKDVAGQFYGVYFKEAKPSTINYAIIKNGTTGI
                     HIEGDGNNGSTPTVTVTNTIIQNNASYGILNFAGGKIKATNCVVSKNGLHGFVNIAGS
                     GFDFEHCTFAGYASGQTQLPVIGISDYYKDNSTVLVTDITGSAKNCIFYGNQDDELVF
                     NLDNVGTNNFTFSNCIIKMKTPSTEPFYSGIKWNEAPQFKNVAEYDFDIPYSSPAVNS
                     GLNLSIPQDIKGRV"
     gene            1525..>1657
                     /locus_tag="PKN22_11585"
     CDS             1525..>1657
                     /locus_tag="PKN22_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNS43392.1"
                     /translation="MKKDIHPADYRLVVFKDMSNDYAFLCPSCAASKETIKWEDGNEY
                     "
BASE COUNT          525 a          283 c          293 g          556 t
ORIGIN      
        1 cttactcttc ctttgatatc ttgcggtatg cttaaattca acccagaatt tacggctggt
       61 gaagagtatg gaatatcaaa atcgtattct gctacatttt taaattgagg cgcttcattc
      121 catttaattc cactgtaaaa tggttcggtg ctaggtgttt tcattttgat gatgcaatta
      181 gaaaacgtaa agttgttggt acccacattg tctaaattaa ataccaactc atcgtcttga
      241 ttgccataaa aaatacagtt tttagcacta ccagtaatat ctgtaaccaa gacagtggag
      301 ttgtctttgt aataatcgct gatgccaatc acaggcaatt gcgtttgtcc gcttgcataa
      361 cctgcaaatg tgcaatgttc aaaatcaaaa ccgcttccag caatgttaac aaaaccatgc
      421 aatccatttt tggatactac acaattggtt gcttttattt ttccacctgc aaagttcagt
      481 atgccgtaac ttgcattgtt ttgaatgatg gtgtttgtaa cggttacggt tggtgtgcta
      541 ccgttgttgc catcaccttc aatgtggatg cctgttgttc cgtttttaat aattgcataa
      601 ttgatggtag aaggtttggc ttctttaaaa taaacaccgt aaaattgtcc agcaacatct
      661 ttgtaatcac tttctaatcg gtctccttga ataaccactt cattatccaa ttgtccttgg
      721 atgttgagtg tgcctttata gacaaataaa aaggattttt tatgcaaata aattttagtt
      781 cccgcttgga tggtgagttg tttgccttca tccacaaatg cagcgccgta aattaagtgg
      841 ggcttatcat taggccaaac accttggttt aaatccaaat tattggtagc accactcatg
      901 tctgaataat ggtagtaaat atcttgtccc catgcagcaa gctgaatata ttgatctttg
      961 ccgttggtac gaaatcgaat ggaatcttca atcaccatgg gcaatgtgcc accattgggg
     1021 tttaacgtaa cttcaacaaa cataaaaagg ctgtcttttg caggaagttc aatttcagaa
     1081 aactttatgc cttgcaaacc atccaaatta atacgaaaag gggaggagtt gccccccatt
     1141 aattctacat ctgaaattaa tacaggtttg ttttctttgt tgtaaatttt gagttgtttg
     1201 gtggtggaac caatggttgt aaaaacagtg tcgaaaacaa cagtatctct agaaaaagct
     1261 aaatttcctt tagaaagcaa tgttgttttc ttacaagaag tattcattga tagaataaat
     1321 aatgcaacaa ctgtaaagaa cgttaattgc catttcagag ctgtttttgt tttgttgatc
     1381 acctgttttt tcatattgat acgcttgtaa aaataacgaa aacaataaaa acttatttta
     1441 tttttgtaaa aatgtaggat aataaaaaaa aatagttacc tttgcaatcc atttttagga
     1501 taaacaagat aataataatt caaaatgaaa aaagatatac atccagcaga ttatagattg
     1561 gtagtgttta aagacatgtc aaatgactat gctttcttat gtccatcttg cgcagcgtca
     1621 aaagaaacaa ttaaatggga agatggtaat gaatatc
//
LOCUS       DAOQVQ010000523         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_99197_length_1651_cov_3.534461, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000523 DAOQVQ010000000
VERSION     DAOQVQ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99197_length_1651_cov_3.534461"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..>1651
                     /gene="ricT"
                     /locus_tag="PKN22_11590"
     CDS             348..>1651
                     /gene="ricT"
                     /locus_tag="PKN22_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNS43393.1"
                     /translation="MGCENCESGEKSSRGCESGACNSSEAKFAVFDWLGNVSLPSNQA
                     PYEILEIRFKNSRKGFYRNNKGLTLAVGNVVVVEASPGYDVGVVSAVGELARIQVNRK
                     EPNFKELEAKKIIRFATQEDIDTWAKGRALEKEVMISSRTLAVNLGLEMKISDVEYQG
                     DLTKATFYYTADGRVDFRQLIKDMAEKFKIRIEMRQIGARQEAARLGGLGSCGRELCS
                     STWLTDFRTVSTSAARYQQLSLNPQKLAGQCGKLKCCLNYELDMYIEALKDFPKAETK
                     LHTEKGIAVHIKTDVFKKQMWYAYTGEAAIGSGLINLSPEKVKEIIALNKNGTKPKDL
                     EGFIEFEETTEEIVDYTNVVGQDSLNRFENAFKSKKKKKKKKPMGAANANPQATSAPT
                     QHKSSEARPQNQNRPKNQPRNNNNNNTGVSNNANNGNNNGNK"
BASE COUNT          588 a          310 c          345 g          408 t
ORIGIN      
        1 tctggacggg actcgaaccc gcgaccccat gcgtgacagg catgtattct aaccaactga
       61 actaccaaac caatttttta accctattgc tagaactcaa caacagttga gcaaagtttc
      121 aaattgtgtg tgcttttcgt taaaagcgtg gggcaaaatt aggcatttat tttaattcgc
      181 aaacaaaaaa atgaaaaaaa ataaaaaaaa attttttcaa gaaaataaac taactgaaaa
      241 tcaaaataat agtatttaaa atatttttaa acctccacaa catattttgc aacaatcgtc
      301 caaatcctat tttttattat taattttaca ctttaaatac agaatttatg ggatgtgaaa
      361 attgtgaaag tggcgaaaaa tcgtcacgag gatgtgaaag cggtgcctgt aattctagcg
      421 aagctaaatt tgcggtgttt gattggctcg gaaatgtatc gttgccatcc aaccaagcgc
      481 cttacgaaat attagaaata cgttttaaaa acagtcgtaa agggttttat agaaataata
      541 aaggtcttac gctggctgtt ggaaatgtag ttgtagtaga agcaagcccc ggttatgacg
      601 tcggtgtggt atcggcggtg ggcgaactgg ctagaattca agtcaaccga aaagaaccaa
      661 attttaagga attagaagcg aaaaaaatta ttcgctttgc tacgcaagaa gatattgata
      721 cttgggctaa aggtcgcgct ttggaaaaag aagtcatgat ttcttcgcgt acactggctg
      781 tgaacttagg attggaaatg aaaatttcgg atgtggaata ccaaggcgac ctcaccaaag
      841 caacgtttta ttatactgcc gacggacgtg tggattttcg ccaattgatt aaagacatgg
      901 ccgaaaaatt caaaatccgc atcgaaatgc gccaaattgg tgcgcgccaa gaagctgctc
      961 gcttgggtgg tttgggctcg tgcggacgtg aattgtgctc ctctacatgg cttaccgatt
     1021 ttagaacggt ttctacttcg gctgcacgtt accaacaatt atcgctcaac ccgcaaaaat
     1081 tggcaggaca atgtggtaag ttgaaatgtt gcttgaacta tgagttggac atgtacattg
     1141 aggcgttgaa agattttccg aaagcagaaa caaaattgca taccgaaaag ggaattgccg
     1201 tacatatcaa aacagacgtt ttcaaaaaac aaatgtggta tgcctataca ggcgaagcgg
     1261 caatcggttc gggactaatc aacctttctc cagaaaaggt caaagaaatc attgcgctca
     1321 ataaaaacgg tacgaaacca aaagacttgg aaggcttcat cgaatttgaa gaaacaaccg
     1381 aagaaattgt ggattacacc aatgtggtgg ggcaagatag cttgaaccgt tttgaaaatg
     1441 cattcaaatc gaaaaagaaa aagaagaaga aaaaacctat gggagctgcc aacgccaatc
     1501 cacaggcaac atctgctcca acgcaacata aatcttcgga agctcgaccg caaaatcaaa
     1561 accgtccaaa aaatcaaccg cgtaacaaca ataacaacaa caccggagta agcaacaatg
     1621 ctaataacgg aaacaataac ggcaataaga a
//
LOCUS       DAOQVQ010000524         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_99482_length_1648_cov_4.126805, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000524 DAOQVQ010000000
VERSION     DAOQVQ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99482_length_1648_cov_4.126805"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKN22_11595"
     CDS             <1..1191
                     /locus_tag="PKN22_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS43394.1"
                     /translation="VVLYNDLAKQRLNTNNLQIKFDYVGKVKSVGYRFYYSFMNDSLY
                     YKDPDYLNAAGELIASYRNKAHDFGVSVGLPLGKYFKLNAGAQEIISHLHSIESLTAS
                     PQRFHFLSFVKSTFNANKWSAVAQVGVQQVNEINRNGDKARNRTRVNPYVDVRYRVHK
                     NISLIAYYRNSFRMPNFSELYYNNIGNNDLKPEDAQQVNLTTAFTILDRNRIYLGIQA
                     SGYYHQIDNMILAIPTKNLFVWSIQNIGKNEIRGADAILSFSWAFGKHWTTQLTANYT
                     FQQSLDRSNKNSPTYGNQIAYAPKHILNGDVSLQWKGVGVRYSNFYSSARYALNENIP
                     ANRIEGFLISDFTFFSRFQIDKHNSVRLQFTLKNITDQSYAYVKSFIMPGRHFLFTFV
                     YEFI"
     gene            1178..>1648
                     /locus_tag="PKN22_11600"
     CDS             1178..>1648
                     /locus_tag="PKN22_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43395.1"
                     /translation="MNLFKIIVIALVLVACKKEQTPDENIPVDFFNNGILVVNEGLFQ
                     QNNSSLTWIDFTSGNSNSEVFEQKTGRQLGDTGNDIARYGNKIYIAVTTSSTLEIIDA
                     KNGNSLKQINMVDNGTPKQPRFITFSNGKAFISCFDGYVDVLDTTSLEITTRIKV"
BASE COUNT          535 a          325 c          270 g          518 t
ORIGIN      
        1 gttgtgttat acaacgacct tgcaaaacaa cgcctcaata caaacaacct tcaaattaaa
       61 tttgattatg taggaaaagt aaaatcagtg ggctatcgct tttattattc atttatgaac
      121 gatagtttgt attacaaaga tcctgattat ttgaatgctg ctggtgaatt aattgccagt
      181 tatcgcaaca aagctcacga ttttggtgtt tcggttggac taccacttgg aaaatatttt
      241 aaactcaacg ctggtgctca agaaataatc agtcatttac attccattga gtcattaacc
      301 gcttctcctc aacgttttca ttttctttct tttgtgaaat ctacttttaa tgccaataaa
      361 tggtcggcag tggcacaagt tggtgttcaa caagttaatg aaatcaacag aaatggagat
      421 aaagctcgca accgcacacg cgttaaccct tacgtggatg tgcgctatcg tgtacacaaa
      481 aatatttcgt taattgccta ttaccgcaat tcttttagaa tgcccaattt tagtgaattg
      541 tattacaaca acatcggcaa taacgacctc aaaccagaag atgcacaaca agttaactta
      601 actacagctt ttacaattct agacagaaac cgcatttatt taggcattca agcaagtggc
      661 tattaccatc aaattgacaa catgatttta gccattccaa ctaaaaactt atttgtttgg
      721 tctattcaaa acattggtaa aaacgaaata cgtggtgccg atgccatcct ttcgtttagt
      781 tgggcttttg gaaaacattg gaccacccaa cttactgcca attatacgtt tcagcaatcg
      841 ttggacagaa gcaataaaaa cagtccaact tatggaaacc aaattgctta tgctccaaaa
      901 catattttaa acggcgatgt ttcattgcaa tggaaaggtg ttggagtgcg ttattccaac
      961 ttctattcat cagctcgtta tgccctcaat gaaaacattc cagccaatcg aattgaaggt
     1021 tttttaattt ccgacttcac attttttagt cgctttcaaa ttgataaaca caacagtgtt
     1081 cgcttacaat ttactttaaa aaacattacc gaccaatcgt atgcatacgt taaaagtttt
     1141 atcatgcctg gtcgccattt cttatttact ttcgtttatg aatttattta aaattatagt
     1201 aatcgcactt gttctggttg cttgtaaaaa agaacaaaca ccagatgaaa atattcctgt
     1261 tgattttttt aacaacggaa ttttagtggt caacgaagga ttgtttcaac aaaacaactc
     1321 ctctctcacg tggattgatt tcacttctgg caactccaat agcgaagttt ttgaacaaaa
     1381 aactggtcgt caattgggcg acactggaaa cgatattgcg cgttatggca acaaaatcta
     1441 cattgccgtt accacctcca gcacgcttga aattattgat gccaaaaacg gaaactcact
     1501 caaacaaatc aatatggtag acaatggaac acctaaacaa ccacgtttta ttacattttc
     1561 aaacggaaaa gcatttatca gttgttttga cggctatgtt gatgtgttag atacaacttc
     1621 gttggaaatt acaacacgca ttaaggtg
//
LOCUS       DAOQVQ010000525         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_99742_length_1646_cov_2.598994, whole genome shotgun sequence.
ACCESSION   DAOQVQ010000525 DAOQVQ010000000
VERSION     DAOQVQ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99742_length_1646_cov_2.598994"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1373)
                     /gene="secY"
                     /locus_tag="PKN22_11605"
     CDS             complement(33..1373)
                     /gene="secY"
                     /locus_tag="PKN22_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNS43396.1"
                     /translation="MKRLIQDLKNIWKVEELRKRIILTLSLILVYRIGSFIIIPGVNY
                     TALAQANQAGSANVLEDLLSMFSGGGFSNASIMALGIMPYISASIIMQLLGMAVPAVQ
                     KMQNEGESGRKQINNYTRILTIIICALQAPSYLALYVRSKGAMPADESTFWWTQTILV
                     LIAGSMFAVWLGERITEKGIGNGISLLITVGILARLPQAFMSEIGINSGNLIKIILEI
                     MVFMVIVMATVFIVQGVRKIPLNTAKKIVGMRELDDAQGARNYLPIKVNASGVMPIIF
                     AQAIVTIPMMLFATNSETPSRLQVEMSNPYGFWYNTLLAVLIIVFTYFYTAIMINPKK
                     IADDLKKSGGFIPGVKPGEDTANYVDDLVSRITLPGSLFLALIAIIPAIAYIAGVNGQ
                     FAMFFGGTSLLIMVGVVLDTLQQIESYLLNRHMDSLMDGTKTKGRRSANEISAY"
     gene            complement(1394..>1646)
                     /locus_tag="PKN22_11610"
     CDS             complement(1394..>1646)
                     /locus_tag="PKN22_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HNS43397.1"
                     /translation="FKNINRVEYKAINLDLIQALVERKNVTEISIEVLRENGLISNND
                     LVKILGRGELTAKINITAHKFSASAKEAIEKNGGVCSEI"
BASE COUNT          567 a          314 c          227 g          538 t
ORIGIN      
        1 cttgttctat tgatgtttgc tttgccatac aactaatatg ctgaaatttc gttagctgat
       61 cttctacctt tagtctttgt tccatccatt aaactatcca tgtgacggtt taataaataa
      121 gactcaattt gttgcaaagt atctaaaact actcctacca taattagtaa agaagtacca
      181 ccaaagaaca tcgcaaattg accgttaaca ccagcaatat atgctattgc aggaataatt
      241 gcaatcaatg ctaaaaataa agaacctggt aatgtaattc tcgatactaa atcatcaaca
      301 tagtttgcag tatcttcacc tggtttaaca cctgggataa atcctccact tttcttcaaa
      361 tcatctgcaa tttttttagg gttaatcata attgcagtat agaaataagt aaatacaatg
      421 attaatacgg ccaaaagggt attgtaccaa aaaccataag ggttactcat ttcaacttga
      481 agtctgcttg gtgtttcaga atttgttgca aacaacatca ttgggattgt tacaattgct
      541 tgagcaaaga taataggcat tacaccagat gcattcacct taattggtaa atagtttcta
      601 gcaccttgcg catcatctaa ttcacgcatt ccaacaatct ttttagctgt atttagtgga
      661 attttacgaa caccttgtac gataaacaca gtagccataa caataaccat gaaaaccatt
      721 atttccaaaa ttattttaat aaggttacca ctgttaatac caatttcaga catgaatgct
      781 tgtggtaggc gagctaaaat acctacagta attaataatg atataccatt accaattcct
      841 ttttcagtaa ttctttcacc caaccaaaca gcaaacatag aaccagctat tagtactaaa
      901 attgtttgtg tccaccaaaa tgtagattca tcagctggca ttgccccttt gcttctaaca
      961 tataatgcta aataactagg tgcttgtaat gcacagatga taatagttag tattcgtgtg
     1021 tagttgttga tttgttttct accagactct ccttcatttt gcattttttg tacagccgga
     1081 acagccatac ctaataattg cataatgata gaagcactaa tataaggcat aattcccaaa
     1141 gccataattg aagcatttga gaaaccacct cctgaaaaca ttgacaacaa atcctctaat
     1201 acatttgcag aacctgcctg attagcttga gctaaagcag tataattaac acctggaatg
     1261 atgataaacg acccaattct ataaacaaga attagagaaa gagttaagat aattctcttt
     1321 cttaactctt caactttcca aatatttttt aaatcctgta ttaaacgttt catataaaaa
     1381 agtgtaaagt tagttaaatt tcagaacaaa caccgccatt tttttcaata gcttcttttg
     1441 ctgaagcaga aaacttatgt gctgtgatat tgatcttagc tgttaactca cctctaccca
     1501 agattttaac taaatcgtta tttgaaatca aaccgttttc acgtaaaact tcaatagaaa
     1561 tttcagtaac gtttttacgc tcaaccaacg cctgaattaa atctaaatta atagctttat
     1621 attcaacacg gttgatattt ttgaaa
//
LOCUS       DAOQVQ010000526         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_100276_length_1641_cov_3.159521, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000526 DAOQVQ010000000
VERSION     DAOQVQ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100276_length_1641_cov_3.159521"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1641)
                     /locus_tag="PKN22_11615"
     CDS             complement(<1..>1641)
                     /locus_tag="PKN22_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNS43398.1"
                     /translation="PHYSLLKKAHELCTSTIKNHPNSVGSSQCTELLATIEEKSINGK
                     VEAHYPSNNAILAKIDFKNVSRIFVQIYASNEDSKFKNYYQPKETIKKLKLISSQEVQ
                     LPSTDDFHEHTTEIALKPLDYGNYVVLYSTNPDKLDDYNEQHWLSNFQVTDLAFSTRP
                     LGNGTSGIQVVNRQTGAPLDNTTVNVYESKYNYKLSKYEGKLLQKYTTNANGWAVLAH
                     SNESYSKIIEVVNGKDVLSTNNSLYKYDNNHEQTYSDHIYTDRAIYRPGQTVYFKGIR
                     LAHNNKKVEIAPNSSVRVGFKDVNYEEVSSLNLTTNEYGSYTGSFRIPTSGLTGEMSL
                     ETEYGSIYFSVEEYKRPTFKVEFEKSKEEYKLGTTVKVEGNAMAYAGNAVDNATVNYV
                     VKRKSYLPFWRAYYYRISPYTSTETIISKGTATTDKEGKFSFSFDAEKDPTHRQGFNY
                     TYTVEADVTDMNGETRSSIKYIHINESAVQLNVSVKDALDKSLENTLTIHASNADGEP
                     VNSKGTIVISKLKSVDELTRSRMWEAPDMPLLSESEFKA"
BASE COUNT          452 a          305 c          292 g          592 t
ORIGIN      
        1 tgctttaaat tcactttcag acaacaaagg catgtcggga gcctcccaca ttcttgatcg
       61 tgttaactca tcaaccgatt ttaatttgga aataacaatg gttccttttg aatttacagg
      121 ctctccatca gcattgcttg catggattgt gagggtgttt tctaggcttt tatccaatgc
      181 atcttttaca gaaacattca attgcactgc actttcattg atgtgaatat atttaataga
      241 cgagcgtgtt tctccattca tatcagttac atcagcctcc actgtatagg tgtaattaaa
      301 cccttgacgg tgcgtagggt ctttttctgc atcaaaactg aatgagaatt tcccttcctt
      361 gtctgtcgtt gccgttcctt tagaaattat tgtttctgtt gacgtgtatg gactaattct
      421 gtaataataa gcacgccaaa atggcaaata cgatttgcgt ttgactacat aattaaccgt
      481 tgcattgtcc accgcatttc cagcgtatgc catagcattt ccttcaactt taacggttgt
      541 tccaagtttg tattcttctt tggatttctc aaattctact ttaaaagttg gacgtttgta
      601 ttcttctaca gaaaaataaa tagatccata ttcggtttcc aagctcattt cacccgttag
      661 tccagacgtt ggaattctaa aactacctgt atatgagcca tattcattgg tagtaagatt
      721 taatgaacta acttcttcat aattcacatc tttgaaccca acacgaaccg atgaattagg
      781 tgcgatttca acttttttat tgttgtgtgc aagacgaata cctttgaaat aaacggtttg
      841 tccaggacga taaattgctc tatcggtata aatatggtct gaatacgttt gttcatgatt
      901 gttgtcgtat ttatacaaac tattgttggt agaaagcaca tctttaccgt ttacaacttc
      961 aatgatttta gaatacgatt cgttggaatg tgccaaaact gcccagccgt ttgcattggt
     1021 ggtgtatttt tggagtaatt tcccttcgta tttgctcaat ttgtagttat attttgactc
     1081 atacacattc accgttgtat tatccaaagg agcacctgtt tgtcggttta ccacttgaat
     1141 tccagaagta ccattgccca acggacgagt agaaaatgcc aaatccgtta cttgaaaatt
     1201 cgataaccaa tgttgttcgt tgtagtcgtc taacttatca gggttggtag aatacaacac
     1261 cacataattg ccataatcta acggttttaa agcaatttca gttgtgtgtt catgaaaatc
     1321 atctgttgat ggaagctgga cctcttgcga ggaaatcagt tttagttttt ttattgtttc
     1381 tttgggttga taatagtttt tgaatttact atcctcattt gatgcataaa tttgcacaaa
     1441 aatacgcgac acgtttttaa aatctatttt agcaagaatg gcattgttag acggataatg
     1501 cgcttccact ttaccattga ttgatttttc ttcaatggtt gctaataact cagtgcattg
     1561 gctacttccc actgaatttg gatggttttt aatagtagaa gtacacagct catgtgcttt
     1621 tttcaatagt gaataatgag g
//
LOCUS       DAOQVQ010000527         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_100560_length_1638_cov_3.696147, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000527 DAOQVQ010000000
VERSION     DAOQVQ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100560_length_1638_cov_3.696147"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PKN22_11620"
     CDS             <1..1476
                     /locus_tag="PKN22_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNS43399.1"
                     /translation="DGYFKLRELDWKGYKLEYLEKVGLVKSKDDRHFDFFRGRVMFPI
                     HSISGRVLGFGGRTLFTDKKIAKYFNSPESIIYNKSEILYGLFFSKGDIIKYDNCFLC
                     EGYSDVISMYQAGIQNVVASSGTSLTKEQIRLIRRYTNNITILYDGDAAGIKASFRGI
                     DLILQEGLNVKVVLFPDGEDPDSYSKKVGSAVLEQYIKEHTQDFISFKAGILLKDTDA
                     DPLKKAELIKDIVHSVGLIPDSITRTVFTQKIAEEFHIQESILNAELLKIRKNVLAKE
                     LNEPEIRELPIHLPVSTESPTPVVSPVEPTIPSVVLEELELIRLMVLYGPIATIAPSV
                     NVHGEKIEVEVSVIQYICDELEHDELTFETPLFAEIHQLYVDGLLNDELYLASFLKRN
                     PNQEVVQLICDIEANELEISNNWYNKHKIDTQRDKDKLATVVPRAIYAFKLFKINQQI
                     SALQKQLTNPELTDAELTDLLGEKIALERIKLKFTSVLGRV"
BASE COUNT          531 a          263 c          328 g          516 t
ORIGIN      
        1 gacggctatt ttaaattaag agagttggac tggaaaggct ataaattaga ataccttgaa
       61 aaagttgggt tggtgaagtc aaaagacgac cgtcattttg atttttttag aggtcgcgtg
      121 atgtttccaa ttcactctat ttctggtcgc gtgcttggat ttggcggtag aactttgttt
      181 accgacaaga aaattgccaa gtatttcaac tcgcccgaaa gcatcattta caacaagagt
      241 gaaattctct acggattgtt cttttcaaag ggcgatatta tcaaatacga caattgcttt
      301 ttgtgcgaag gctattcgga tgtgattagc atgtatcaag caggcattca aaatgtggtg
      361 gcttcttcgg gtacatcgtt gaccaaagaa caaattcgtt taattcggcg ctataccaac
      421 aatattacca ttttatacga cggtgatgcg gctggtatta aagcttcgtt tagaggaata
      481 gacctgattt tacaagaagg cttgaacgtt aaagtagtgt tgtttcctga tggtgaagac
      541 cccgattctt actctaaaaa agtaggaagt gcagtattgg aacaatacat taaagagcat
      601 acgcaggatt ttatatcgtt caaggctgga attttattga aagatacgga tgctgatcca
      661 ctcaaaaaag ccgagttaat taaagatatt gttcattccg tagggctgat tccagactct
      721 atcactcgaa ctgtttttac gcaaaaaatt gctgaagaat tccacattca agaatccatt
      781 ttgaatgcag aattactaaa aattcgcaaa aacgttcttg ccaaagagtt aaacgaacca
      841 gaaattaggg agcttcctat tcatttgcct gtttctactg aaagtcctac accggttgta
      901 tctccagtag aaccaacaat accttccgtt gtattagaag agttggaatt gatacgttta
      961 atggtgttgt atggacccat tgccaccatt gcgccaagtg tgaacgtgca tggcgaaaaa
     1021 atagaggtag aagtttctgt tattcaatac atttgcgatg aattggagca cgatgaatta
     1081 acctttgaaa cgccactttt tgccgaaatt caccaattgt atgtagatgg tttgctcaac
     1141 gatgaactgt atttagcttc gtttttaaaa cgcaatccca accaagaagt ggtgcaatta
     1201 atttgtgata ttgaagccaa tgaattggaa atcagtaata attggtataa taagcacaaa
     1261 attgatacac aacgtgataa agataagtta gcaacagttg ttccgcgggc aatttacgct
     1321 tttaagttgt ttaaaatcaa tcaacaaatt agtgcgttgc aaaaacaatt gacaaatcca
     1381 gaattaaccg acgcagagtt aaccgactta ttgggcgaga aaatagcatt ggaacgcatt
     1441 aagctgaagt ttacatcggt tttaggtcga gtgtaggtaa aaggttttta aaggttaagt
     1501 ttgttagaca ctgatttaag taaatttttc aaaagaacaa ttgatttcta aagattaaat
     1561 tacttctgca atatcgaaat atgtcgatat gtaaatttct aattattatt ttgagtttta
     1621 aaattttatt tttatctt
//
LOCUS       DAOQVQ010000528         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_101009_length_1634_cov_3.259658, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000528 DAOQVQ010000000
VERSION     DAOQVQ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101009_length_1634_cov_3.259658"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PKN22_11625"
     CDS             <1..1569
                     /locus_tag="PKN22_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS43400.1"
                     /translation="VKEVVGELKAEVDKKSNAVPYNPVMVPNVSSKLDEYFPMISPDN
                     ELMFFTRKVDDKALGEMTSYWVEKFTVAKRDKMTLPFDQGTFLKAPFNDGSFDNYGAS
                     TLSVDNKEMIVCACKTELVYEQKYKNCDLYITTYERTGSGVNDFKWSELKNMGPAINT
                     KDGWEGQPSLSADGNTLYFATTRKGSQDNDIYFSTRNEEGEWSQAKPLAILNTPGKDK
                     SPFLHQDSETLYFVSDGRKDGMGGLDIYYTRKNEKGEWDTPKNIGYPINTEADEIGIF
                     VSIDGKLAYFSSRQGGNWNIYGFELYEEARPKSVAIIKGDLASENGEPIENATIEIAY
                     ENSTEVTKVKVNGNDGKYAAIVKTDKPQDVMVTVKKEGYAFDSKLISKEEVKEVATSK
                     DVAIRGNDLNVKELKVGEAYTINDILYATNSYALTEKSKFILRGFARFLKENPTIKVA
                     IQGHTDDVGDDNQNLVLSDNRAKGVRDYLISLGINGSRLSAKGYGETQPKVENVSDEN
                     RAKNRRTDFVIESL"
BASE COUNT          600 a          273 c          336 g          425 t
ORIGIN      
        1 gtaaaagaag tggtgggcga attaaaagca gaagtagata aaaaatccaa cgctgttccc
       61 tacaatccag tgatggttcc aaatgtgagt tccaaattag atgaatattt tcctatgatt
      121 tctcccgaca acgagttgat gttttttact cgcaaagtgg acgacaaagc attgggtgaa
      181 atgacctcat attgggtaga gaaattcacc gttgccaaac gagataaaat gaccttgcct
      241 tttgatcaag gaacgttttt aaaagctcca tttaacgatg gctcttttga taactatgga
      301 gcaagtacgc tttctgtaga taacaaagaa atgattgttt gtgcttgtaa aaccgaactg
      361 gtgtacgagc aaaaatacaa gaattgcgat ttatacatta caacctacga acgtacaggt
      421 tcgggggtta acgattttaa atggtctgaa ttaaaaaaca tgggacctgc aatcaatacc
      481 aaagatggat gggaggggca accttcactt tcggcggatg gaaacacctt gtattttgca
      541 accactcgca aaggttctca agacaatgat atttattttt caacccgcaa tgaagaagga
      601 gaatggtcgc aagcaaaacc attagccatc ctcaacacac caggaaaaga taaaagtcca
      661 ttcttacacc aagacagtga aacactttat tttgtctccg acggaagaaa agacggaatg
      721 ggtggtttgg atatttacta cacccgaaaa aatgaaaaag gtgaatggga cacacctaaa
      781 aatattggat atcccatcaa cacagaagca gatgaaattg gaatttttgt gtccattgat
      841 ggtaaattag cctatttttc atcacgtcag ggggggaatt ggaacattta tggttttgaa
      901 ttgtacgaag aagcacgtcc aaaatcggtg gctatcataa aaggtgattt ggcaagtgaa
      961 aacggtgaac caatagaaaa tgcaaccata gaaattgctt acgaaaattc aactgaagta
     1021 accaaagtaa aagtaaacgg aaacgacggt aaatatgcag ccattgtgaa aaccgacaaa
     1081 ccacaagatg taatggtaac ggttaaaaaa gaaggttatg catttgattc taagctaatt
     1141 tccaaagaag aagtgaaaga ggttgcaact tcaaaagacg tggcaattcg tggcaatgat
     1201 ttgaatgtaa aagaattaaa agtgggtgaa gcttatacca ttaacgatat tttatatgca
     1261 accaactcgt atgcattgac cgagaaatcg aagtttatac ttagaggttt tgcacgtttc
     1321 ttaaaagaaa acccaaccat aaaagtagcc attcaaggac ataccgatga tgtgggtgat
     1381 gataaccaaa acttagtgct ttcagacaat cgtgctaaag gtgtacgcga ttatttaatc
     1441 agccttggaa ttaatggaag tcgcttatcg gctaaaggat atggtgaaac acaaccaaaa
     1501 gttgaaaacg tttcggatga aaacagagct aaaaaccgta gaaccgactt tgtaatcgag
     1561 agtttataag caaaatttaa tacctttgta aaaaatcaat accatgaaaa agttaatcta
     1621 tttattacca atta
//
LOCUS       DAOQVQ010000529         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_102202_length_1623_cov_3.935587, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000529 DAOQVQ010000000
VERSION     DAOQVQ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102202_length_1623_cov_3.935587"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="prs"
                     /locus_tag="PKN22_11630"
     CDS             <1..409
                     /gene="prs"
                     /locus_tag="PKN22_11630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNS43401.1"
                     /translation="NSYAKRLDTGLAICHKQRKKANEVAEMTVIGDVEGKDVVLVDDM
                     CDTAGTLTKAADLFMSKGAKSVRAFCTHAVLSGPAYERIEQSNLTELIVTDTIPLKQQ
                     SSKIRVLSVAELFADVINKMVHNQSISSHFVIS"
     gene            422..991
                     /locus_tag="PKN22_11635"
     CDS             422..991
                     /locus_tag="PKN22_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNS43402.1"
                     /translation="MKKAQLSGSARANVGKKDAKALRNQGLVPAVLYGTGEQTHFAVK
                     ANDINKIVFSPDVYNVELDIDGKKANAIIRELQQHPVKDTIQHVDFYEVTDKKPVKIG
                     LPVRLEGTARGVLAGGKLLQVFRKLNVYGIAKDLPEAITVKINDLRIGQSIRVKDVQI
                     EGVTILDPANAVIVSIKKSRNSVDDSGDE"
     gene            1124..>1623
                     /locus_tag="PKN22_11640"
     CDS             1124..>1623
                     /locus_tag="PKN22_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNS43403.1"
                     /translation="MYIIFDTETTGLPRNWNAPITDTDNWPRVVQLAWQLHDAMGNVI
                     EHKDFLVKPEGFDIPYDAERIHGISTELATTYGESLQTVLAEFNIALSKAKFVVGQNI
                     QFDINVMGCELHRTNIESPMLQMPVLDTCTEVTANLCKIPGGRGGRFKLPTLTELHQF
                     LFNVPFA"
BASE COUNT          548 a          285 c          320 g          470 t
ORIGIN      
        1 caactcgtat gccaaacgtt tggatacagg tttagcaatt tgccacaagc aacgtaaaaa
       61 agccaatgaa gtagccgaaa tgaccgtaat tggtgacgta gaaggaaaag acgttgtatt
      121 agttgacgat atgtgcgaca cagcaggaac gttaacaaaa gcagccgact tatttatgtc
      181 taaaggcgca aaatcggttc gtgctttctg tacacacgca gtgctatctg gtcctgcata
      241 cgaacgcatt gaacaatcca acttaacgga attgattgta accgacacca ttccattaaa
      301 gcaacaaagc agtaaaatca gagtattatc tgtcgctgaa ttgtttgccg atgttatcaa
      361 caaaatggta cacaatcaat ccattagttc acactttgta atatcttaaa taaataataa
      421 aatgaaaaaa gcacaattga gcggttctgc gagagcgaac gtagggaaaa aggatgctaa
      481 agcacttaga aatcaaggtt tagttcctgc agtgctttat ggtacaggtg agcaaactca
      541 ctttgctgta aaggcaaacg atattaataa aatcgttttt tcaccagatg tttataacgt
      601 agaattagac attgatggta aaaaagcaaa cgcaattatc cgtgagttgc aacaacaccc
      661 agtaaaagat actattcaac acgttgactt ttacgaagta actgataaaa aaccagttaa
      721 aattggttta ccagttcgtt tagaaggtac agcaagaggt gtattagctg gtggtaaatt
      781 gttacaagtt tttagaaaat tgaatgttta tggtattgct aaagacttac cagaagcaat
      841 tacagttaaa attaacgact tgagaattgg acaatctatc cgtgtgaaag atgttcaaat
      901 cgaaggagta acaattttag atcctgctaa cgcagtgatt gtttcaatta agaaatctcg
      961 taactctgtt gatgattcag gtgatgaata attaaagagt tcattctttt tgaaaagtcc
     1021 ttctcgtttt ttcgggaagg atttttttgt taataaactt ttgaagactg cctaaaaatg
     1081 aattgtaaaa attcgtaatt ttatacttca caaaaaatca tccatgtaca tcatatttga
     1141 caccgaaacc acaggattac caagaaattg gaacgctcct atcaccgata ccgacaattg
     1201 gccacgcgtt gttcagttag cttggcagtt gcacgatgca atgggaaatg taattgagca
     1261 caaagatttt ttggtcaaac cagaaggatt tgatatccct tatgatgctg aacgtataca
     1321 tggaatttct acagaattgg ctacaaccta tggagaatcg cttcaaacgg ttttagcaga
     1381 attcaatatt gcgctttcga aagcaaaatt tgttgttgga caaaacattc aattcgacat
     1441 caatgtgatg ggttgcgaat tgcaccgaac caacatagaa tctccgatgt tgcaaatgcc
     1501 tgtcctcgat acttgtacgg aggttacagc caatttgtgt aaaatacctg gtggtcgtgg
     1561 tggacgtttc aaattgccta cattaacgga attgcaccag tttctattca acgttccgtt
     1621 tgc
//
LOCUS       DAOQVQ010000530         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_103775_length_1608_cov_3.670316, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000530 DAOQVQ010000000
VERSION     DAOQVQ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103775_length_1608_cov_3.670316"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKN22_11645"
     CDS             <1..734
                     /locus_tag="PKN22_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS43404.1"
                     /translation="LLPFCCIYSTFLQRAYDQVIHDVLLQNLKVVFCIDRAGLVGADG
                     ATHHGIFDLAYLRILPNIIIAAPMNESELRNLLYTAHLAAYEGAFAIRYPRGKGVLAE
                     WKQEFETIEIGKGRLLTPGNDLAVLSIGHVGNIVQNTLKNVQNCSVAHYDMRFAKPLD
                     EVLLHEIFKKFTKIITIEDGCVTGGFGSAVLEFMNKHNYSSKIECLGIPDYFINHGTQ
                     EELYRECGFDETSLLQKIYTLQQLR"
     gene            879..>1608
                     /locus_tag="PKN22_11650"
     CDS             879..>1608
                     /locus_tag="PKN22_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS43405.1"
                     /translation="MKYILFLLSLAFIIGCSPKNKSELSGGEIRMCIDSEPLTHISYE
                     VSDYYSSVVFGQVMEGLVSMDPENFKITPQIAKSWEINDDGTIYTFDIHPDVYFHEHS
                     VFSSKSDRLLKISDIIKTFEAICSPNSKNQPTVAYSFILKDNLLGAKEFFEKKSKTIK
                     GIKEENGKLVIELIHKDDNFLNKLSQIHLAIQSEKIINANAVSDIVGTGPFRYFKYNN
                     GEPPSIVLAKNEAYYEVDEQGKQLP"
BASE COUNT          560 a          245 c          284 g          519 t
ORIGIN      
        1 gtttgttgcc tttttgttgc atctactcta cttttttaca acgtgcctat gatcaagtga
       61 ttcacgatgt attgttgcaa aatttaaaag ttgttttttg catagacaga gcgggattgg
      121 ttggtgctga tggggcaaca caccacggta tttttgacct tgcatactta agaattttgc
      181 ccaacattat tattgctgca ccgatgaatg aaagtgaatt gagaaattta ctttatacag
      241 ctcacttggc tgcgtatgaa ggtgcttttg ctattcgcta tccgcgcgga aaaggcgttt
      301 tagcggaatg gaaacaggaa tttgaaacaa ttgaaatcgg taaaggtagg ttgttaactc
      361 ctggcaacga tttggcggtg ttatcaatcg ggcatgttgg aaatattgtt caaaacacac
      421 tgaaaaacgt tcaaaattgt tcagtagcgc attacgatat gcgttttgcg aagccgttag
      481 acgaagtgtt attacacgaa atctttaaaa aattcactaa aatcataaca atagaagatg
      541 gttgtgtaac aggaggattt ggtagtgctg ttttagaatt tatgaataaa cacaactact
      601 cttcaaagat tgaatgttta ggtattccag actattttat aaatcatgga acccaagaag
      661 aactctatcg agagtgtggg tttgacgaaa catctttgtt gcaaaaaata tacacattac
      721 aacaattgag ataaacaaca cataaaacct attcacaaaa aatttttaat aaataattct
      781 tgaaataagt ttttatatct aatgtttttt actaatttta taatctaaat aaatttaacc
      841 acgcattaat taaactaatt gaaaggacta tataacttat gaaatacata ctttttttac
      901 taagtttagc atttatcata ggctgttcac cgaaaaacaa atcggaatta agtggaggcg
      961 aaatccgcat gtgcatcgac tctgagccat taacgcatat ttcctatgaa gtttcagatt
     1021 actattcatc cgttgtattt gggcaagtaa tggaaggatt ggtgagtatg gatcctgaaa
     1081 actttaaaat aactcctcaa attgcaaaat catgggaaat aaatgatgat ggtacaattt
     1141 atacgtttga tattcaccca gatgtttatt ttcacgagca ttctgttttc tccagtaaaa
     1201 gtgatcgttt attaaaaatt tcagacatca ttaaaacgtt tgaggctatt tgttcaccca
     1261 attcaaaaaa tcaaccaaca gttgcttata gttttattct aaaagacaac ttactgggtg
     1321 ctaaagagtt ttttgagaaa aaatcaaaaa ccataaaagg aattaaggaa gagaatggca
     1381 aattggtgat agaacttatt cataaggacg ataacttcct aaataaatta tcacaaattc
     1441 atttagctat tcagtcagaa aaaattatca atgctaatgc tgtgtcagac atagttggaa
     1501 caggaccatt tcgttatttt aaatacaaca atggtgaacc tccttcaatt gttttagcaa
     1561 aaaatgaagc ttattatgaa gtagatgaac aaggaaaaca gcttcctt
//
LOCUS       DAOQVQ010000531         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_104746_length_1599_cov_3.465026, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000531 DAOQVQ010000000
VERSION     DAOQVQ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104746_length_1599_cov_3.465026"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKN22_11655"
     CDS             <1..1088
                     /locus_tag="PKN22_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNS43406.1"
                     /translation="LAPVSQNVVLYIGLATSLVAAFIAMRQNDIKKVLAYSTVSQLGL
                     MFVGLGLGAYTAALFHVTTHAFFKALLFLGSGSVIHGMSGEQDIRKMGGLKKYMPTTH
                     WTFLVGTLAITGFPLLSGFFSKDEISGFAFQHGIPLYLILMFSILLTAIYMFRMYFLT
                     FHGEFRGTEEQKHHLHESPLNMTLPLMVLAVLSIVGGVINLPGLFLKNQAHYLDHLLE
                     KNVAGMNLIHTEHLSHGTILALMVVASLVCIVTLFVTYNVYVKKKSMPKEESALTGWE
                     KLSANKLYVDELYNAIIVKPILWLSESGYRVVELVIIDGLIMKAAKGVGSAGEVVKKW
                     QSGQVNWYVLWMVSGIVGLIIYFLVKI"
     gene            1093..>1599
                     /locus_tag="PKN22_11660"
     CDS             1093..>1599
                     /locus_tag="PKN22_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNS43407.1"
                     /translation="MELLLIPFVVALLALLVPQKAVKVTALIGALVTVVVGLAYLVGY
                     KPSADFISLIPACSESCSKPFFMSYGYDGVGLTMVVLTNIIVFLILLSNYDNDKFSTS
                     KRFNALIFFMQFALIGVFLSLDGLLFYIFWELSLIPVFLLFLWYGEKEKRTALMKFFI
                     YTFIGSLAM"
BASE COUNT          441 a          248 c          332 g          578 t
ORIGIN      
        1 aattggctcc agtttcacaa aatgttgtgt tgtacatcgg tttggctact tcattggttg
       61 cagcatttat tgccatgcgt caaaacgata tcaaaaaagt attggcttat tcaacggtat
      121 cgcaattagg attaatgttt gttggacttg gattgggagc ttacacagcc gcacttttcc
      181 atgttacaac tcacgcattc ttcaaagctt tgttgttctt aggttcgggt agcgtaattc
      241 acggtatgtc gggcgaacaa gatatacgta aaatgggtgg cttgaaaaaa tacatgccaa
      301 ctacccattg gacattcttg gtgggaactt tggcaattac tggtttcccg ttattatctg
      361 gtttcttctc aaaagacgaa atttcaggtt ttgctttcca acacggcatt ccattgtatt
      421 taattttgat gttctctatc ttgttaactg ccatttatat gtttagaatg tatttcttaa
      481 cattccacgg tgagtttaga ggaacagaag aacaaaaaca ccatttacac gaatcaccat
      541 taaacatgac tttaccgttg atggttttgg cggtgttgtc aattgttggt ggagttatca
      601 atttaccagg acttttcctt aaaaatcaag cgcattactt ggatcatttg ttggagaaaa
      661 atgtggcagg gatgaattta attcacactg agcatttatc gcacggaaca attttagcgt
      721 tgatggttgt tgcttcattg gtttgtattg ttacgctttt tgttacatac aacgtatatg
      781 tgaagaaaaa atcaatgcct aaagaggaaa gcgcattaac tggttgggaa aaattatcag
      841 caaacaaatt atatgttgat gaattgtaca atgcaataat tgtgaaacca attttatggc
      901 tttctgaaag cggttaccgt gtagtagaat tggtcatcat tgatgggttg attatgaaag
      961 cagctaaagg cgtaggttct gcgggtgagg ttgtgaaaaa atggcaatct ggtcaagtca
     1021 attggtacgt tctctggatg gtttcaggaa ttgtaggttt aattatttat ttcttagtaa
     1081 aaatttaagg ttttggaatt actattaata cctttcgttg ttgcgcttct tgcgctgttg
     1141 gttcctcaaa aagcagttaa agtaactgca ttaatcggag cattggttac tgtagttgta
     1201 ggtttggctt atttggtagg ctataaacca tctgcagact tcatttcttt aattcctgca
     1261 tgttcagaaa gttgctcaaa accattcttt atgagttatg gttatgatgg agtagggttg
     1321 accatggttg ttttaaccaa catcatcgtt ttcttaatct tattgagcaa ttatgataac
     1381 gataaatttt caacttctaa acgtttcaac gcattgattt tcttcatgca atttgcatta
     1441 atcggtgtgt ttttatcgtt ggatggtttg ttgttctata ttttctggga attgtcgtta
     1501 attccagtat tcttattgtt cttatggtac ggtgagaaag aaaaaagaac tgccttgatg
     1561 aaatttttca tctacacatt catcggttca ttggcaatg
//
LOCUS       DAOQVQ010000532         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_104755_length_1599_cov_3.207902, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000532 DAOQVQ010000000
VERSION     DAOQVQ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104755_length_1599_cov_3.207902"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKN22_11665"
     CDS             complement(<1..282)
                     /locus_tag="PKN22_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNS43408.1"
                     /translation="MKLLVKNAKIVDATSSHNNTTKDILIENGTITAISESISDSDAT
                     VIAHDNLHISQGWVDLKADFCDPGAEYKETVQSGLDAAVFGGYTHVAVLP"
     gene            complement(282..>1599)
                     /locus_tag="PKN22_11670"
     CDS             complement(282..>1599)
                     /locus_tag="PKN22_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43409.1"
                     /translation="LFFKVTNHGKNDLQNVQLQFETGDIKRDLFIDIAHQNSTVSSIS
                     FSPKKNGIQTGVLKVADKQFYADDDFYFTYNVVEKSAILVVNGEDANSNLANIYRLDN
                     FYAVNEVSQNELIQSMLQGVNLVVLNGVNDIPTGFNSMLSEYANNGGAVAVFLGKNVK
                     TNSVNPFLSKLGLPMVVKEIAQATRINKVNYKDVFFKGVFDKEKDQLSLPGVTKFYLV
                     NENSSANAIGIVHLQNGKSLLYRTLKNQQAFLFTSVLQPEYGSFVSDILYTTIVLRMG
                     ELSLHNPPLSLTIGLTNNYPIYHKIDTEKPVVILGKSLEFIPMTIRKGAVTYIDLSGN
                     EAVSNLTSGVYSLENNGNVIGQIALNYNRAESQIATWEKDAIENALKQAGIENVSYMN
                     VDKGVHSVNLNIEKPYPYWKFFISFALLFLVAELLILKLWKNKIEN"
BASE COUNT          471 a          311 c          277 g          540 t
ORIGIN      
        1 tggcaaaaca gccacgtgtg tgtaacctcc aaaaaccgct gcgtccaatc cactttgtac
       61 tgtttctttg tattctgctc ctggatcaca aaaatcggct ttcaaatcaa cccaaccttg
      121 cgagatgtgt aaattgtcgt gtgcaataac agttgcatca ctgtcagata ttgattccga
      181 aatagcggta atggtgccat tctcaattaa aatgtctttt gttgtgttgt tgtgacttga
      241 agttgcgtcg actatttttg cgttttttac cagtaatttc atcaattctc aattttattt
      301 ttccacaatt tcagtattaa gagttctgcc actagaaata gtaaggcaaa agaaataaaa
      361 aacttccaat atggatacgg tttttctatg tttaaattaa cagaatgcac tcctttatca
      421 acattcatgt aagaaacgtt ctcaattcct gcttgtttta gggcgttttc aatagcatct
      481 ttttcccatg ttgcaatttg cgattcggct ctgttgtaat ttaacgcaat ttgaccaatg
      541 acattgccgt tgttttccaa cgaataaact cccgatgtta aattagaaac agcttcgtta
      601 ccactcaaat caatgtaggt tacagctcct ttgcgaatgg tcatcggaat gaattccaac
      661 gatttaccta agataacaac tggtttttca gtgtctattt tatgataaat tggataattg
      721 ttcgtgagcc cgatagtcag tgaaagtggg ggattgtgga gtgataattc tcccattctc
      781 aatacaatag ttgtgtacaa aatatcggaa acaaacgacc catattcggg ttgcaacaca
      841 ctggtaaaca aaaatgcttg ttggtttttt aatgtgcgat acaagagcga ttttccattt
      901 tgcaaatgca cgataccaat ggcgttggcg ctagaatttt cattcaccaa gtagaatttt
      961 gttactcccg gcaagctcaa ttggtctttt tctttgtcaa aaacgccctt gaaaaaaaca
     1021 tctttataat tcaccttgtt gatgcgagta gcttgtgcaa tttcttttac taccattggt
     1081 aaacctaatt ttgatagaaa tggattaaca ctgtttgttt ttacattttt tcctaaaaac
     1141 acagcaactg caccaccatt gttcgcatat tctgaaagca tggagttgaa ccctgtcggg
     1201 atgtcattaa caccattgag caccaccaaa ttaacgcctt gcaacatgct ttgtatcaat
     1261 tcgttttggc tcacctcatt caccgcataa aaattatcca agcggtaaat attggcaagg
     1321 ttagaatttg catcttcgcc gttaaccact aaaatagccg atttttcaac cacattgtag
     1381 gtgaaataaa aatcatcgtc ggcataaaat tgtttatcgg caacctttaa aacacctgtt
     1441 tgaatgccat ttttcttggg tgaaaatgaa atggatgata ccgtagaatt ttggtgagca
     1501 atgtcaataa ataaatctcg ttttatatcg cccgtttcaa attgcaattg cacattttgt
     1561 aaatcgtttt ttccgtggtt ggttaccttg aaaaacaat
//
LOCUS       DAOQVQ010000533         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_106111_length_1587_cov_2.748042, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000533 DAOQVQ010000000
VERSION     DAOQVQ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106111_length_1587_cov_2.748042"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..829)
                     /locus_tag="PKN22_11675"
     CDS             complement(320..829)
                     /locus_tag="PKN22_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43410.1"
                     /translation="MEIKFSKYQGAGNDFILVDNRDGRYNSLTDKQIQFLCDRRFGIG
                     ADGLIKINQHTAFDFEADYYNADASKSFCGNGARCAVAFCQTLGIDVKNTSFLGFDGP
                     HKAAFDNNEIQLQMNSVEQLKLHDSDYEIYTGSPHYIRFVNNVKDFDIFSLIHINYIY
                     FGLYHYLNY"
     gene            complement(860..>1587)
                     /locus_tag="PKN22_11680"
     CDS             complement(860..>1587)
                     /locus_tag="PKN22_11680"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNS43411.1"
                     /translation="GAKIMYAAKVTDKTFEQISNTSALTTGAKEQIASGRLDSMVMKD
                     LGIYVIDRGYDYDGNQQGYFKVEVLENSATTFKGRFANIDGSNDQTITLQKNAEYNYV
                     FVKWNPTGAVTNPIIEPKKDSWDIVFTQFSKVFYEPEFTPYVVVGCLTNPYKTLSCYA
                     EGKTFDEINLTVAENTPLSTDWDAIGYDWKSFSLNQSMYDVYYNKVYLIKDQNDYYYK
                     LRFVDFYSDSGEKGSPTFEYQRL"
BASE COUNT          559 a          242 c          250 g          536 t
ORIGIN      
        1 gaatataata aataaagtac aagaatcaaa acaatacttc aaaaaaatat tagatattta
       61 agaggtaaaa atatgaaaaa atcaatttta aaagaatagt atacaattaa aagatttaaa
      121 gaatagaatc caaatttaaa taggaatata aaacttaaat tagaagaaga attttctgat
      181 gttacattag aagatgttct taatgtatgt attaaaagtg atccaacgta tagtgaaaat
      241 tcaaatgaag tgggtaattt ttagtattta ttgttattat tattttctaa ttcaaatggt
      301 ggagtaattg agaaatatgt taatagttca gataatgata taaaccaaaa tatatataat
      361 ttatatgaat taatgaaaaa atatcaaaat ctttcacatt gtttacaaaa cgaatgtagt
      421 gaggagaacc tgtataaatt tcgtaatcgg aatcgtgtaa ttttagctgt tcaaccgaat
      481 tcatttgcag ttgaatttca ttgttatcaa acgctgcctt gtgcggaccg tcaaaaccta
      541 aaaaagaggt gtttttaacg tcgattccaa gtgtttgaca aaaagctaca gcacaacgag
      601 caccatttcc acaaaaactt ttggaagcat cggcattgta gtaatccgct tcaaaatcaa
      661 atgcagtatg ttggttgatt tttatcaaac catcggcacc aattccaaat ctgcggtcac
      721 aaagaaattg tatttgttta tcggttaacg aattgtaacg accatctcgg ttgtcaacta
      781 agataaagtc atttccagct ccttggtact ttgaaaattt aatttccata gtaacaaaga
      841 taatgaaatg ttgaaaatat tacaggcgtt ggtattcaaa tgttggcgaa cctttctcac
      901 cactatcgct gtaaaaatct acaaaacgca atttgtaata atagtcattt tgatctttga
      961 tgaggtacac tttgttgtaa tacacgtcat acatactttg atttaatgaa aaacttttcc
     1021 agtcataacc aattgcatcc caatcagttg ataaaggtgt gttttctgca acggttaaat
     1081 ttatttcatc aaatgttttt ccttctgcat agcaagatag ggttttgtat ggatttgtta
     1141 agcatccaac aacaacataa ggtgtaaatt ctggttcgta aaatactttt gaaaattgtg
     1201 tgaaaacaat gtcccacgaa tcttttttag gttcaataat tggattggta acagcacccg
     1261 taggattcca tttcacaaaa acatagttgt attctgcatt tttttgaagt gtaattgttt
     1321 gatcgttact tccatcaatg ttggcaaaac gccctttaaa agttgtagca gaattttcta
     1381 atacttccac tttaaaatat ccttgttggt tgccatcgta atcgtaacct cggtcaataa
     1441 cataaatacc caaatctttc atcaccattg agtctaatct acctgaggca atttgttcct
     1501 tggcaccagt cgttaaagca gatgtattgc taatttgttc aaatgttttg tcggtaacct
     1561 tggcggcata cataattttt gcaccat
//
LOCUS       DAOQVQ010000534         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_106990_length_1579_cov_3.333990, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000534 DAOQVQ010000000
VERSION     DAOQVQ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106990_length_1579_cov_3.333990"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1579
                     /locus_tag="PKN22_11685"
     CDS             155..>1579
                     /locus_tag="PKN22_11685"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNS43412.1"
                     /translation="MSCFVTISLSLVAQTTITYQSTMNPNNLVNNILLGYGVTASNVT
                     FNGAGGSTMQGNAKSFTATNFPFSSGVYIRTSGGSSVASDADLNAIATNNVTNGGKLE
                     FDFVAQGNNLSFEYMFASAEYPTYVCSGFNDVFGFFISGPGITGPYTNGAVNMALIPN
                     TNVPVAINTVNSGTAGTAGTASTCAAQDPNWQSNSVYYTTQYATYSGEGYNGGTVNLP
                     AGISLQCGETYHIKIAVANVGDQALNSGVYLKANSFNSDAVQVVVATVTGDTTIYEGC
                     SDANIYFIRPSSQNDTTLTINYTIGGTATNGVDYNHLADSVTFNIGVDTVVVNITPGA
                     DGISDNNETVTITATIVNACGDTLTSTGTLVILDSVDVKLNFTDPTVFCINDSVPVIA
                     SAYNGFGPYTYAWSNGDVGDTGYLATVDTTQGSIQYIVTATDACGYTGSDTVTVTVNQ
                     TLFIDSILTTPANCQPIGTVSATSF"
BASE COUNT          497 a          271 c          297 g          514 t
ORIGIN      
        1 atattatttt ttttagcaaa tttgcttaat ttctagcaaa aaagaaagga agttttatat
       61 ttttgaaaaa gttaatgcaa cttaactaag aaatattacg tttcaaaact aaataactag
      121 tacgatgata aacttttata ataaattaat agctttgagt tgttttgtaa caatttcgct
      181 ctcacttgtg gcgcaaacaa ccatcaccta tcaaagcaca atgaatccta ataacttggt
      241 taacaatatt cttttgggat atggagttac tgcatccaat gttactttta atggagctgg
      301 aggatctact atgcaaggta atgcaaaaag ttttactgca accaactttc cgttttctag
      361 tggtgtttac attcgtacaa gtggtggttc atccgtagct tcagatgcag atttgaatgc
      421 catagcaacc aataatgtta ccaatggtgg taagttagaa tttgattttg tggcgcaagg
      481 gaacaattta tcttttgaat acatgtttgc atcggcagaa tatccaactt atgtatgctc
      541 tggatttaat gatgtttttg gattttttat ttcaggacct ggaataactg gaccttacac
      601 caatggagct gtaaacatgg ctttaattcc taatacaaat gttccagttg caattaatac
      661 tgtaaattct ggtaccgcag gaacagcagg aacagcatca acgtgtgcgg cacaagatcc
      721 aaactggcaa tcaaattctg tgtattacac tacacaatat gcaacctata gtggagaagg
      781 atataacggc ggtacagtga atttacctgc tggtatttct ttgcaatgtg gtgaaaccta
      841 ccatataaaa attgctgttg caaatgtggg agatcaagct ttgaattcag gggtttacct
      901 taaagcaaat tcatttaact cagatgctgt tcaagtagtg gtagcaactg taacaggtga
      961 cacaacaatt tatgagggct gtagcgatgc aaatatttat tttatccgac catcaagcca
     1021 aaatgatacg actttaacta ttaattatac tattggagga acagctacaa atggtgttga
     1081 ttacaatcat ttggctgatt ctgttacttt taatattgga gttgatacag ttgtcgttaa
     1141 cattacacct ggtgcagatg gaatttcaga taacaatgaa acagtaacta ttactgcaac
     1201 tattgttaat gcttgtggtg atacccttac atctactggt acgctagtca ttctagactc
     1261 ggtggatgtt aagctaaact ttactgaccc aactgtattt tgtattaacg attcggttcc
     1321 agttattgca agcgcataca atggttttgg tccatacacc tatgcttggt caaatggaga
     1381 tgtgggagat acaggatatt tagctactgt tgatacaaca caaggttcta ttcaatacat
     1441 tgttaccgca accgacgctt gtggatatac tggaagtgat acagtgactg ttacggtcaa
     1501 tcaaacattg tttatagatt ctatcctaac aactcctgcc aactgtcaac caattggaac
     1561 agtttcagca acatcgttt
//
LOCUS       DAOQVQ010000535         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_107791_length_1572_cov_3.128543, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000535 DAOQVQ010000000
VERSION     DAOQVQ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107791_length_1572_cov_3.128543"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..634
                     /locus_tag="PKN22_11690"
     CDS             89..634
                     /locus_tag="PKN22_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43413.1"
                     /translation="MRIFVNQYICIMEKPLYHYFTDDHRQIEAILEKAIENIEQVNDT
                     YYQEFRARLLTHIKMEEKILFLAAQKANNNVPLPLAAQLRLEHGAITSLMVPSPTPEL
                     IKVLKHVLEIHDIKEEEEGGMYDACEAMTVQQREEILEQLGNVTLTPIHPHNDAPIAL
                     EAAKRALLRAGFDYDKIVAEQ"
     gene            699..>1572
                     /locus_tag="PKN22_11695"
     CDS             699..>1572
                     /locus_tag="PKN22_11695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS43414.1"
                     /translation="MKIYIVGSAHPLRGGGISTFNERLAEVLQNEGHDVTIISYKKQY
                     PSLLFPGKSQYTNEPAPKGLKIRSLIHSYNPFNWLKVGRILQKEKPDVIVSRYWLPFF
                     GPCLGTIHRIVRKNKHTKIIALLDNVIPHEHRIGDNALTNYFLPSVQGFITMSKEVMA
                     DVKKFNATQPVEFAPHPMYDNYGALMSRQEALNQLNLSPDFSYLLFFGFIRDYKGLDL
                     LLEALSLVKNQQVKLIVAGEFYNNKATYEQIIQKNKLEDKLILHTDFIPNDQVSLYFS
                     AADVVVQPYRTATQS"
BASE COUNT          547 a          282 c          281 g          462 t
ORIGIN      
        1 ataaaacaac aaacactgat aaattacggc tttcagaaat ggaagccgtt tttttgtttt
       61 ttaatattca taaataacta ccataattat gcgtattttt gttaaccaat acatctgcat
      121 catggaaaaa ccattgtacc attattttac cgacgaccat cgccaaatag aagctatttt
      181 agaaaaagca attgaaaaca tcgaacaagt taacgatacg tattaccaag aatttagagc
      241 gcgtttgttg acacacataa aaatggaaga aaaaattttg tttttggcag cacaaaaagc
      301 caacaacaat gtgccattgc cattagcagc tcaattgcgt ttagagcacg gagccatcac
      361 atcgttaatg gtgccatcgc ccactccaga gttgattaaa gtgctaaaac acgtgttgga
      421 aattcacgat ataaaagagg aggaagaagg aggaatgtat gatgcttgtg aagcaatgac
      481 cgtgcaacaa cgagaggaaa ttttagaaca attgggcaac gttaccttaa ctcccattca
      541 tccacacaac gatgctccta ttgcccttga agctgcaaaa agagctttgt tgcgcgccgg
      601 atttgattac gataaaatag tagcagaaca ataaaacaat tgggctattg tgttgattgg
      661 tgttctattt caaacttttg gttatatctt tgtgaaggat gaaaatatac atagtaggct
      721 cagcccatcc attaagaggt ggaggcattt caacatttaa tgaacgttta gcagaagtat
      781 tgcaaaatga aggacacgat gtaaccatca tttcctataa aaaacaatac ccttctttgt
      841 tgtttcctgg aaaaagccaa tacaccaatg aaccagcacc caaaggttta aaaattagaa
      901 gtctcatcca ttcatacaat ccattcaatt ggttgaaagt aggacgcatc ttgcaaaaag
      961 aaaaaccaga tgttattgtt tcacggtatt ggttgccttt ttttggacct tgtttgggaa
     1021 ctattcatcg tattgttcgc aaaaataaac ataccaaaat tatagcatta ttggacaatg
     1081 ttattccgca cgaacataga attggtgata atgcactaac taattatttt cttccttcgg
     1141 tacagggttt tatcaccatg agcaaagaag ttatggcaga tgtgaaaaaa tttaatgcaa
     1201 ctcaaccagt agaatttgct ccccatccga tgtatgataa ttatggagca ttaatgtcgc
     1261 gccaagaagc attgaaccaa ttaaacttat cacccgattt ttcatacctg ttgttttttg
     1321 gttttattag agattacaaa ggtttagact tgcttttgga ggcattgtct ttggtaaaaa
     1381 atcaacaagt aaaattaatt gtagcaggag aattttacaa caacaaagca acatacgagc
     1441 aaattattca gaaaaataag ttggaagaca aattgatttt acacaccgat tttattccaa
     1501 acgatcaagt ttcactttat ttttcagctg ctgatgtggt ggtccaaccc tatcgaacag
     1561 caacacaaag tg
//
LOCUS       DAOQVQ010000536         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_109407_length_1558_cov_3.764471, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000536 DAOQVQ010000000
VERSION     DAOQVQ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109407_length_1558_cov_3.764471"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1546)
                     /locus_tag="PKN22_11700"
     CDS             complement(158..1546)
                     /locus_tag="PKN22_11700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNS43415.1"
                     /translation="MNQTQQYIQVNKERFLTELLDLLKIPSISADKAYSQDVKKTAEL
                     IAEKFTTIGLDKVEVCPTAGNPIVYGEKIIDASLPTILVYGHYDVQPADPIELWDNPP
                     FEPIIKKTAIHPEGAIFARGACDDKGQMYMHVKAVEAMLATNELPCNVKLMIEGEEEV
                     GSTNLSIFVKENVEKLKADIILVSDTGMLANDTPSITTGLRGLSYVEVEVTGPNRDLH
                     SGLYGGCVANPINILTKMIASLHDENNRITIPGFYDKVVELSDEERAEMAKAPFSLEK
                     YCDALDIREVHGETGYSTPERGAIRPTLDVNGIWGGYIGEGAKTVIPSKAFAKISMRL
                     VPDQNSDEITELFKKHFESIAPASVKVAVHPHHGGEASVTPIDSVGYKAASMAYEKAF
                     GKKPIPVRSGGSIPIVAMFEKVLGLKTIMMGFGLDSDAIHSPNEHYGLFNYYKGIETI
                     PYFYKHFTELNK"
BASE COUNT          456 a          336 c          241 g          525 t
ORIGIN      
        1 agggttaagt taaacgacag tgataaatca cgaccagata gtttttcaaa ttttaaacca
       61 tcaactccaa catagctaac attttcaata gtacaactat taaaaacaaa taggagtgcc
      121 acaagacacc cccatttcaa tatatttttc gatacaatca tttgtttaat tctgtaaaat
      181 gcttgtagaa atatggaatc gtttcaattc ctttgtaata attaaacaaa ccataatgct
      241 catttggcga gtgaatagca tcactgtcca atccaaatcc catcataatg gttttcaatc
      301 ctaatacttt ttcaaacata gcaacaattg gaatacttcc accacttctt accggaatag
      361 gttttttgcc aaatgctttt tcgtaagcca tgcttgctgc tttgtatcca accgaatcta
      421 tcggagtaac ggaagcttca ccaccatgat gtggatgaac agctactttt acactagcag
      481 gagcaatgga ttcaaaatgt tttttaaaca attctgtaat ttcatccgaa ttttgatccg
      541 gtaccaaacg cattgatatt tttgcaaatg cttttgatgg aatcactgtt tttgcacctt
      601 ctccaatgta tccaccccaa attccgttga catccaatgt cgggcgaatt gcacctcttt
      661 ctggagttga ataacctgtt tcaccatgca cttctcggat gtccaacgca tcacaatatt
      721 tttctaatga gaaaggagct tttgccattt ctgcacgttc ctcatccgat aattccacca
      781 ccttatcgta aaaaccaggg atagtaatgc ggttgttttc atcgtgtaaa gaggcaatca
      841 tttttgttaa aatattaatc ggattggcaa cacatccacc gtataagcct gagtgtaaat
      901 cgcggtttgg accagtaact tccacttcca catagctcaa cccacgcaaa cctgttgtaa
      961 tggatggcgt atcatttgcc aacattccag tatccgaaac taaaatgata tctgctttta
     1021 atttttccac attttctttc acaaaaatgg aaaggttggt gcttcccact tcttcttctc
     1081 cttcaatcat taacttcacg ttgcacggta attcattcgt agccaacatc gcctcaaccg
     1141 ctttcacgtg catgtacatt tgtcctttgt catcacaagc accacgtgca aaaattgctc
     1201 cttcgggatg aatggctgtt ttttttatga ttggttcaaa tggtggatta tcccacaatt
     1261 caatagggtc tgctggttgc acatcatagt ggccataaac caaaatggtt ggtaaactcg
     1321 catcaattat tttttctcca taaacaattg gatttccagc tgttggacaa acttctacct
     1381 tgtcgagacc aatagttgta aatttttcag caattaattc tgccgttttt ttcacatctt
     1441 ggctatatgc cttatctgct gaaattgaag gaatttttaa caaatctaac aattccgtta
     1501 aaaaacgttc tttattaact tggatatact gttgtgtttg attcatatta tttttttt
//
LOCUS       DAOQVQ010000537         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_109544_length_1557_cov_3.248336, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000537 DAOQVQ010000000
VERSION     DAOQVQ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109544_length_1557_cov_3.248336"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKN22_11705"
     CDS             <1..587
                     /locus_tag="PKN22_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNS43416.1"
                     /translation="KFSEQQKIKAKGKTIKEITIGYDALAVVVNTGNKVTKLTREQLE
                     GIFTGKIKNWKEVGGADLKIIPLSRETSSGTYEFFKEKVMGNKNYMSGIRSLPATGAI
                     IQSVTQTKGAIAYVGFAYLNSSVKALSVSFNSGKSYTKPSVENAKNKTYPIVRPLYYY
                     YLTSSESKVKDFVNYVLSTEGQKIVQTVGYIPAK"
     gene            606..>1557
                     /gene="pstC"
                     /locus_tag="PKN22_11710"
     CDS             606..>1557
                     /gene="pstC"
                     /locus_tag="PKN22_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNS43417.1"
                     /translation="MNKKKAIKKIIDKVIEKLFTISGATTSIVIVFICIFLFKEGVGL
                     FKTPTVEQGYGLFVNSKNTVEQLSPEQIKQLFDGEISNWKTVGGPQKDIKLLRVDDIF
                     AIYPESEMGENFELLPEKLKEQLVKEENAIGFFPLEFAPTGAAIKQLPTDHLKWKDYF
                     FGKEWLPTATPSPLFGALPLIYGTLLVSLFALIIAVPLGLGVAIYLSELATERQRKIM
                     KPIIELLAGIPSVVYGFFGLVVLAPLIQKTFNLPSGETAFTGSVLLAIMALPTIISVA
                     EDAMRNTPRAMKEASLALGATHWQTIYRVIIPYSKSGIMAA"
BASE COUNT          533 a          255 c          300 g          469 t
ORIGIN      
        1 ttaaattttc ggaacaacaa aaaattaaag ctaaaggaaa aactatcaaa gaaattacaa
       61 ttggctacga tgcacttgca gttgttgtta acacaggaaa taaggtaaca aaacttacac
      121 gcgagcaatt ggaaggcatt tttacaggaa aaattaaaaa ctggaaagaa gtaggaggtg
      181 ctgacttgaa aattattcct ttatcgcgcg aaacatcttc tggaacctat gaatttttca
      241 aagaaaaagt aatgggtaac aaaaattata tgagcggtat tagaagttta cctgcaacgg
      301 gagcaattat tcaatctgtt acgcaaacaa aaggagcaat tgcctatgtt ggttttgcct
      361 acctcaacag ttctgtaaaa gcattatcgg tttcatttaa tagtggaaaa tcatacacga
      421 agccaagtgt tgaaaatgcg aaaaacaaaa cgtatccgat tgttagacct ttgtattatt
      481 actatttgac aagttcagaa tcaaaagtta aggattttgt caactatgtg ctttcaacag
      541 aaggtcaaaa aattgtgcaa actgtaggtt acattccagc aaaataaacg ctttttaagt
      601 taaccatgaa caaaaagaaa gcaataaaaa aaatcattga taaagtaatt gaaaaacttt
      661 tcacgattag cggtgccacc acaagcatcg ttatcgtgtt tatatgtatt tttcttttca
      721 aagaaggagt tggattgttt aaaacaccaa ctgtggagca aggttatgga ctttttgtga
      781 attccaaaaa cacggttgag caattatctc cagagcaaat aaaacaacta tttgacggtg
      841 aaatcagtaa ttggaaaact gttggcggac cacaaaagga cattaagtta ctacgtgttg
      901 atgacatttt tgcgatatat cctgaaagtg aaatgggtga aaattttgag ttattgcccg
      961 aaaaattaaa agaacaatta gtaaaagaag aaaatgccat tggatttttt ccattagaat
     1021 ttgcgccaac gggagccgca attaagcaat tgccaactga tcatctaaaa tggaaagatt
     1081 acttttttgg aaaagaatgg ttgcctactg ctacaccttc tcctttgttt ggcgcacttc
     1141 ctttaattta tggtactttg ctcgtaagtt tatttgcgtt gataattgct gtgccacttg
     1201 gtttgggtgt tgccatttat ttgtcggaat tagcaacaga acgtcaacgt aaaatcatga
     1261 aacccatcat tgaacttttg gcaggaattc cgtcagtagt ttatggtttt tttgggttgg
     1321 ttgttcttgc cccactcatt caaaaaacat ttaacttgcc aagtggtgaa actgctttta
     1381 caggtagtgt tttattggca attatggcct tacctactat tatttcggtg gcagaagatg
     1441 ctatgcgcaa tacaccaaga gccatgaaag aagccagctt agcattgggt gctactcatt
     1501 ggcaaaccat ttacagagtc atcattcctt attcaaaatc gggaataatg gctgcca
//
LOCUS       DAOQVQ010000538         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_111090_length_1544_cov_2.871054, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000538 DAOQVQ010000000
VERSION     DAOQVQ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111090_length_1544_cov_2.871054"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..>1544)
                     /locus_tag="PKN22_11715"
     CDS             complement(17..>1544)
                     /locus_tag="PKN22_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS43418.1"
                     /translation="RYKSEERLKWEVEFDCINKFKEWILSFNADGNKIAVEADLKAIE
                     EEAKKHVRDSKNTAWKDFSDTLKVDFDAAMGLLKAVAESSSNKAFVQKEVDALEKAMN
                     PIRKDILSTARKVLRITRNEKTSQRATLAQWIQNSVHENYDRYSSHLYSQSKKSALNI
                     EAIAPTYTADAESVDGRIVLRDNYDKLLEKYPEVLVFGEDVGKIGGVNQTLEGMQEKY
                     GELRVSDTGIREATIIGQGIGMAMRGLRPIAEIQYLDYLLYAVQVMSDDLATVQYRTK
                     GGQKAPLIVSSRGHRLEGIWHSGSPMGMIINSLRGIHVCVPRNMTKAAGFYNLLLESD
                     EPALVIEPLNGYRSKELYPSNIGEFKIALGVPEITREGNDLTLVSYGSTFNLCTVAAQ
                     QLAELGISVELIDAQTLLPFDIHHVITESLEKTNRLMIVDEDVSSGATAYMLDKILVE
                     QNGYYHLDSAPVTVSAKDHRPAYSTDGDYFSKPSIDDIVEAAYGLMHEADPASFPSMY
                     "
BASE COUNT          458 a          332 c          244 g          510 t
ORIGIN      
        1 aatagtaaat attctattaa tacattgatg gaaaacttgc tggatctgct tcgtgcatta
       61 gcccataagc cgcctcaaca atgtcatcga tacttggttt tgagaaataa tcgccatctg
      121 tactataagc aggacggtga tcttttgcac tcactgttac aggagctgaa tccaagtggt
      181 aataaccatt ttgttcaact aaaattttgt ccaacatata tgctgttgcg ccactgctca
      241 cgtcttcgtc aacaatcatc aaacgatttg ttttttccaa cgattctgta atcacatggt
      301 gaatatcaaa aggaagcaat gtttgagcat caatcaattc aacactgatt cctaattctg
      361 ccaattgttg tgcggcaacg gtgcataaat taaaagttga accgtaagaa acaagtgtca
      421 aatcatttcc ttcacgagtg atttctggta cacccaatgc aattttaaat tctccaatat
      481 tggaaggata caattctttt gaacggtaac cattcaacgg ttcaataacc aatgctggtt
      541 catcgctttc caacaataaa ttgtagaaac ctgcagcttt agtcatgttg cgaggcacac
      601 aaacgtgaat tccacgaagt gagttaataa tcattcccat tggtgaacct gagtgccaaa
      661 tcccttccaa acggtgacct ctagaactta caattaacgg tgctttttgt ccgccttttg
      721 tacggtactg cacagttgcc aaatcgtctg acatgacttg tacggcatac aataaataat
      781 ccaagtattg aatttcagca ataggacgca aacctctcat tgccatacca attccttgtc
      841 caataatggt tgcctcacga attccggtat ccgaaacgcg caattcacca tatttttctt
      901 gcattccttc caatgtttgg ttaacgccac caattttacc aacgtcttca ccaaaaacaa
      961 gcacttcagg atatttttct aataatttat cgtagttatc acgcaataca attcttccat
     1021 ccaccgattc tgcatctgct gtataagttg gtgcaattgc ttcgatattt aaagcagatt
     1081 ttttagattg agaatacaaa tgagaactat agcggtcgta attttcatga acactatttt
     1141 gaatccattg tgctaaagta gcacgttgac ttgttttttc attgcgtgta atgcgcaaaa
     1201 ctttacgagc tgtagaaaga atgtctttac gaataggatt catcgctttt tcaagcgcat
     1261 ccacttcttt ttgaacaaat gctttattgg agctagattc agcaactgct tttaacaatc
     1321 ccattgcagc atcaaaatca acttttaagg tatcagaaaa atctttccat gccgtgtttt
     1381 tactatctct tacgtgtttt tttgcttctt cttcaatggc ttttaaatct gcttcaactg
     1441 ctattttatt accatctgca ttaaaactca aaatccactc tttgaattta ttgatgcaat
     1501 cgaattcaac ttcccatttc aaacgttctt cagatttgta acgt
//
LOCUS       DAOQVQ010000539         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_111674_length_1539_cov_2.652291, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000539 DAOQVQ010000000
VERSION     DAOQVQ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111674_length_1539_cov_2.652291"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PKN22_11720"
     CDS             complement(<1..1306)
                     /locus_tag="PKN22_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNS43419.1"
                     /translation="MGLLSLNYKTILSVALPMMGASFVQSIVLLTASAFLSRYSTDAF
                     AAVGNAGLIFITMHIFVVGISDGTQILISNKIGKRNTSDIGRLLLTSILVLTIIVSLL
                     VAFAQLFLPDLILGYAKHAEIALAQIDFITIRVYGLFFAMILLPLQAYYFAFGKSWVP
                     FVGAILTAISNIILDYCLIFGNWGFPEMGIEGSAWAAVVSDGVDAVFLVSYFFLSKEH
                     IRQKIFENFKLVKSTLVEIFRMSIPIVFQGSVSLLTWAIFFIWIEQMGKFELTVSQNI
                     RTVYFLAFVPVWGFAAATKTYVSQYSGAKRVRDIIVVRRRIQLLLLLFMFIIFHGGIF
                     YPETVISWINPEYAYVPKSAETLRYISLSMFIFAYFSVFFHTINGLGKTHITFIVESI
                     AVFLYIASAYVFIKLMRLDVKYIWTVEYIYYISMGILSITYLK"
BASE COUNT          590 a          275 c          226 g          448 t
ORIGIN      
        1 attttaaata ggtgattgat agaataccca ttgaaatata gtaaatgtac tcaactgtcc
       61 aaatgtattt tacatccagt ctcattagtt taatgaatac ataagccgat gcaatgtaca
      121 gaaatacagc tatagactca acaataaaag tgatatgggt tttacccaaa ccattaattg
      181 tatgaaaaaa cacactgaaa taagcaaaaa taaacatgga taaactgatg taccgtaatg
      241 tttcagcgct ttttggaaca taggcatatt cgggattaat ccatgaaatc actgtttctg
      301 gataaaaaat ccctccatga aaaattatga acatgaagag caaaagtaac aattgaattc
      361 ttcttctaac aacaataata tcacgcacac gttttgctcc cgaatattga gaaacatagg
      421 tttttgtggc tgcggcaaat ccccaaactg gtacaaatgc taagaaatac accgttcgaa
      481 tattttgtga aaccgttaac tcaaatttcc ccatttgttc aatccaaatg aaaaagatag
      541 cccaagttag caacgacact gagccttgaa acacaatggg aatagacatt ctaaaaattt
      601 caactaaggt tgatttcacc aatttaaaat tttcaaatat tttctgacga atgtgttctt
      661 ttgacaagaa aaagtagcta actaaaaata cagcatccac tccatccgaa acaactgctg
      721 cccaagccga accttcaatt cccatttctg gaaatcccca gttcccaaaa atcaaacaat
      781 agtctaaaat aatattgctt attgcagtta aaatagctcc aacaaaaggc acccatgatt
      841 ttccaaaagc aaaataataa gcttgcaagg gcaataaaat catagcaaaa aacagcccat
      901 agacacgaat ggtaataaaa tctatttgag ctaaagcaat ttcagcatgt tttgcatatc
      961 ccaagattaa atcaggtaaa aacaactgcg caaaagcaac caaaagtgaa acgataatcg
     1021 ttagcaccaa aatagaggtt aataacaacc gaccaatatc ggaggtattg cgtttaccta
     1081 ttttgttgga aattaatatc tgagttccat cacttatacc taccacaaaa atgtgcatgg
     1141 taatgaatat caaaccagca ttacctacag ctgcaaaggc atcggttgaa tatcgactta
     1201 aaaatgcaga ggctgttaac aaaacaattg actgcacaaa agaagctccc atcattggaa
     1261 gtgcaaccga aagtattgtt ttgtaattaa gcgaaagtaa tcccaagggt tttattttaa
     1321 atttatgttt atttattaaa cgattacaaa actaaaattt taatctagtt aaatgcttat
     1381 tttttgacat agaacaattt taaaaaatca gtattattgt aaagcaatca aatgcattat
     1441 tgcttatttt ttagtgaaat ttgttttgat taatgaacta caatcctaac attgtcttga
     1501 ttttatctgc aatttcttgt gtgtaataat taggagcac
//
LOCUS       DAOQVQ010000540         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_112009_length_1536_cov_4.238352, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000540 DAOQVQ010000000
VERSION     DAOQVQ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112009_length_1536_cov_4.238352"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..943)
                     /locus_tag="PKN22_11725"
     CDS             complement(38..943)
                     /locus_tag="PKN22_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNS43420.1"
                     /translation="MKNTFYILLFILLYSATSSVFAQTTAINKLEVLYAQGFYKKVYK
                     QAGKLMDKPENDHTMLPSYYRAISALQLSGDAKWARKNQLEIEQSLAALEKLQSLQEG
                     RDILENHQDEMIALKQDLTNRLESLKRDNKTNEADKIAGIIQHYFGSIKEITDTKINI
                     EKPVVKEENFTFNSKNREEIVAYAQKFIGVKYSSSGESPATGFDCSGFTSYVMDAYKI
                     ELPRRSADQYTNAKKLKENQVKKGDLVFFDSGNGVNHVGIIISDEGQSPVMIHSSTSK
                     GIIITDINSSEYWKKRVKGYGTYLN"
     gene            1095..1536
                     /locus_tag="PKN22_11730"
                     /pseudo
     CDS             1095..1536
                     /locus_tag="PKN22_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084974.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          451 a          259 c          284 g          542 t
ORIGIN      
        1 tctatttttt aagctttagt tatgggcatg ataaaactta attaagataa gtgccatacc
       61 ctttaactct ttttttccaa tattctgaag agttgatgtc ggtgatgata attccctttg
      121 atgtgctgga atgaatcata acgggcgatt gtccttcgtc tgaaataata ataccaacat
      181 gattgacacc gtttccactg tcgaaaaaaa ccaagtcacc tttttttact tgattctctt
      241 ttaatttttt tgcattggta tattggtcgg ccgaacggcg aggtaattct attttgtagg
      301 cgtccattac ataagaagtg aatccagagc agtcaaaacc agtagctggc gactcaccac
      361 ttgaactata tttcacacca ataaattttt gtgcgtaagc aacaatttct tccctatttt
      421 ttgaattaaa agtgaagttt tcttctttta caacgggttt ctcaatgttg atttttgtgt
      481 cggttatttc tttgatagaa ccaaagtagt gttggatgat tcctgcaatt ttatccgctt
      541 cattcgtctt gttgtcgcgt tttaaacttt ctaaacggtt ggttaaatct tgtttcaatg
      601 ctatcatttc atcttgatga ttctccaaaa tgtctcttcc ttcttgaagc gattgtagtt
      661 tttctaatgc agcaagcgat tgctctattt ctaattgatt ttttctagcc cattttgcat
      721 cacccgataa ttgtaatgca ctaattgctc tataataact tggtagcata gtgtggtcgt
      781 tttctggttt gtccattaat ttaccagctt gcttgtacac ttttttataa aaaccttggg
      841 catatagcac ttctaattta ttgatagctg ttgtttgtgc aaaaacagaa cttgttgcag
      901 aataaagcaa aataaaaagg agaatataaa acgtattttt catactacaa aaaaacggaa
      961 aaacacgcta cctagtatgt gttttatggc tttatggcta acaatcactt gttcattatt
     1021 taattttctg ccactttgtc gcaatttttg gtttggtaca aatgttgtca aacgaagggt
     1081 aaatttttaa tagcatgaaa acaggtcaaa taaatgtaca aacggaaaac atttttccga
     1141 taattaaaaa gtttttatac tcagatcatg agatttttct acgcgaattg gtttccaatg
     1201 ccgttgatgc gacacaaaaa ttaaagtttt ttgctaaaaa tggcgagttt aaaggtgaat
     1261 taggtaacct gaaagtggaa gtgattctaa ataaggaaga aaaaacactg actattcgcg
     1321 acaacggtat cgggatgact gccgaagaaa ttgataaata tatcaaccaa attgcttttt
     1381 cgggtgccga ggagttatgg ccaagtataa gaataccgaa agtggcatta tcggacattt
     1441 tggcttgggc ttctacagct cgttcatggt ttcgaagaag gttgagatta ttacccgttc
     1501 gttcaaggag ggtgcaactg cacttcgctg gagctg
//
LOCUS       DAOQVQ010000541         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_112305_length_1534_cov_2.711968, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000541 DAOQVQ010000000
VERSION     DAOQVQ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112305_length_1534_cov_2.711968"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PKN22_11735"
     CDS             complement(<1..911)
                     /locus_tag="PKN22_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNS43421.1"
                     /translation="MRKTILIASLLLAFYSQANNHKVISKQEYVDMWSRTAMENMLNY
                     KIPASITLAQGVLESGSGNSDLAREANNHFGIKCHNSWTGPRMYKDDDQKNDCFRKYA
                     SADLSYKDHSEFLTNSKRYANLFTLEITDYKSWAKGLKEAGYATNPKYPTLLIDLIES
                     LELYKYDELAMNTPNKNVLVKQNHKNNEKTSAPKVTHESKKKVPVSKVEKQEVEVVFG
                     ATRETFLHDNKVKYVIAKKGDTYFKIAQEFQMTLGQIENYNDVKNGNTTIKEGDIINI
                     HPKQAKGKEAYKTYTEDLTLLQIAQKEG"
     gene            complement(1066..>1534)
                     /locus_tag="PKN22_11740"
     CDS             complement(1066..>1534)
                     /locus_tag="PKN22_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNS43422.1"
                     /translation="IGEFVEGKFLKITDRKKEIFKTSGGKYIIPQFMENTFKESRFIE
                     QMMVIGEGQKFPAALIVPSFAFIKDWAERKGMDLRNASNQQIIENKEVIARIDREVKE
                     YNKRFGNWEQIKKFELLPTEFTIEGGELTPTLKFKRKKILEKYQTEFNRIYND"
BASE COUNT          437 a          283 c          203 g          611 t
ORIGIN      
        1 ccttcttttt gagcaatttg caaaagtgtt aaatcttctg tatatgtttt gtaagcttct
       61 tttccctttg cttgttttgg atgtatgttg ataatgtctc cttctttaat agttgtatta
      121 ccatttttta catcgttgta attttcaatt tgacccaatg tcatttgaaa ctcctgtgca
      181 attttaaaat aagtatctcc ttttttagca atcacatatt ttaccttatt atcgtgtaaa
      241 aatgtttctc ttgttgctcc aaaaactacc tccacttctt gtttttcaac ttttgataca
      301 ggaactttct tttttgattc atgggtaact ttaggagcac ttgttttttc gttgttttta
      361 tggttttgct ttaccaacac atttttatta ggcgtgttca tcgccaattc atcgtatttg
      421 tacaactcca acgattcaat taaatcgata agcaaggttg gatatttagg attggttgca
      481 tagcccgctt cttttaatcc ttttgcccac gatttataat ctgttatttc taaagtaaat
      541 aaattagcat atcgctttga attggtcaaa aactcactgt gatctttata cgacaaatcg
      601 gccgatgcat attttctaaa acaatcgttt ttttgatcgt cgtctttata cattctagga
      661 ccagtccaac tattatggca ctttatacca aaatgattgt tagcttcacg tgctaaatca
      721 gagttaccac tccccgactc taaaacgcct tgagctaatg taatacttgc tggtattttg
      781 taattgagca tgttttccat agcagttctg ctccacatat ctacatactc ttgttttgaa
      841 attactttat gattgtttgc ttgtgagtaa aacgcaagca aaagactggc aattaaaatt
      901 gtttttctca taattattgt tgtttgttaa caatcaatac gtaggttatc aacaaatgtt
      961 tcatttattg ttgaaaacat tggaaaataa tttgcaatca cctttgaatc aacaaaaaag
     1021 gaagctaaaa atacgcttcc ttctttaaat aatattcaat ctatattaat cgttataaat
     1081 tcggttaaat tcagtctgat atttctcaag aattttttta cgtttaaatt tcaatgttgg
     1141 tgttaactct ccaccttcaa ttgtaaattc tgttggcaac aattcaaact tcttaatttg
     1201 ctcccaattt ccaaaacgtt tgttgtattc ttttacttct ctatcaatac gtgcaatcac
     1261 ttctttattt tcaataattt gctgattgga agcatttcgt aaatccattc cttttctttc
     1321 agcccaatct ttaataaaag caaaacttgg aacaatcaaa gcagctggga atttttgtcc
     1381 ctcaccaata accatcattt gctctataaa acgcgattct ttgaacgtat tttccataaa
     1441 ctgcggaata atgtatttac cgcccgatgt tttaaaaatt tcttttttac gatcggtaat
     1501 tttcaagaat tttccttcca caaattcacc gata
//
LOCUS       DAOQVQ010000542         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_112886_length_1529_cov_4.035278, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000542 DAOQVQ010000000
VERSION     DAOQVQ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112886_length_1529_cov_4.035278"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..>752
                     /locus_tag="PKN22_11745"
     CDS             432..>752
                     /locus_tag="PKN22_11745"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNS43423.1"
                     /translation="MKNAFFLLVATIALFSCGGKNNTQQTAYGPQKVDTTKAISVQEF
                     FNDFEQVDVSKAYTIKGKIVETCKKSGCWVGIDDGNNDYFVVNFKDHFSIPLDTKIGT
                     TAYMH"
     assembly_gap    753..762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            910..>1529
                     /locus_tag="PKN22_11750"
     CDS             910..>1529
                     /locus_tag="PKN22_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43424.1"
                     /translation="MGPRSIRISIILGVFSILSIFIVQFIWTKKNVAIQERKIVIQEK
                     KDSLNLIHFSEQVHIALRNTLEQIQTDKSDSSDLYGAVKQVDINYYTVDINEELHPFY
                     LETLLKREFYNQYVREDFQYGIYDCFTDSIVFGNMIKFSEDSLYSPTNEQSLINTYPN
                     LKWEKKDGHYFTVFFPNINDDVAKIEPNTFSSWWYLVFVGIFPLLF"
BASE COUNT          537 a          239 c          260 g          483 t
ORIGIN      
        1 tttaaaatat aaaaatatca taaacgctgc attgttgttt aagacttagt tattatgact
       61 tgatgtgctt ttggaaaact aataaaatag ctaatcttat ttagggtata atatttagat
      121 ttattggtta aacatacaat taattcaaca aataacttat tttatattac ggaataattc
      181 aagtaaacat taaaaagaaa catttcgggc aagaagacca tgtgtgcatt tactcggtgg
      241 taggactaac cgaacgtcta aaatcagttg gttttactgt agaacaacaa acatttgaag
      301 aagatgacta cttagggtta aataaaggcg acattgtact taaattaaca aaaaaatagg
      361 ttaaaagtga tttatctcat aaattgaacc tttaaaaaca gttaactttg tagaaattca
      421 aaatgtagat catgaaaaat gcattctttt tattggttgc aaccatcgca ttgttttctt
      481 gtggtggaaa aaacaacaca caacaaactg cttatggacc tcaaaaagtg gatacgacca
      541 aagctatctc agtacaagaa ttttttaatg attttgagca agtagatgta tctaaagctt
      601 ataccatcaa aggaaaaatc gtagaaacat gtaaaaaatc aggttgttgg gttggaattg
      661 acgatggcaa taacgattat tttgtggtta attttaaaga ccatttttcc attcctttgg
      721 atacaaaaat tggaacaact gcctacatgc atnnnnnnnn nnatggtgtg gcaacttggg
      781 actctatacc agttgatgtt ttaaaagaac aagcattgat ggatggaaaa tcgcaagaag
      841 aagtggatca aattaccgaa ccacgctttg aattcaattt tgaagcagac ggcataatat
      901 tgaaataacg tgggaccacg ctctatccgc atttcgatta ttttgggggt gttttccatc
      961 ctcagtattt ttattgttca atttatttgg acaaaaaaaa atgtggctat ccaagaacgt
     1021 aaaattgtta ttcaagaaaa aaaagacagc ctcaacctca tccatttttc tgagcaagtt
     1081 cacattgccc tgcgcaatac actggaacaa atccaaaccg ataagtcgga tagttctgat
     1141 ttatacggag ctgtaaaaca agttgatatc aactactaca cagtagatat caacgaagaa
     1201 ttgcacccat tttatttgga aacactttta aaacgtgagt tctacaacca atatgtgcgc
     1261 gaagattttc aatacggaat ttacgactgt ttcaccgatt ccattgtgtt tggaaacatg
     1321 attaaatttt ctgaagattc gttgtatagt ccaaccaacg aacaaagttt aatcaacacc
     1381 taccccaact tgaaatggga gaaaaaagat ggacattatt tcaccgtttt ttttccaaac
     1441 attaatgatg atgttgccaa aattgagccc aacacatttt cttcttggtg gtatttggtg
     1501 tttgttggaa tttttccgct attgttttt
//
LOCUS       DAOQVQ010000543         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_112888_length_1529_cov_3.820217, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000543 DAOQVQ010000000
VERSION     DAOQVQ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112888_length_1529_cov_3.820217"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..862
                     /locus_tag="PKN22_11755"
     CDS             266..862
                     /locus_tag="PKN22_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS43425.1"
                     /translation="MAMKNLDDSILLSDYINGNEKAFEVLVLRHKDKIYRYIYLKVKN
                     QLLAEDIFQETFIKAIRTIKLGAYNEDGKFLPWIMRIAHNLAIDHFRKTNKIKLISET
                     SSKNEDFNIFNTLQLNELNIQDQISLDELKTQMVDLVEYLPDSQKDIIKMRIFQDLSF
                     KEIADLENISINTALGRMRYALINLRKLIEKHQLVTDI"
     gene            924..>1529
                     /locus_tag="PKN22_11760"
     CDS             924..>1529
                     /locus_tag="PKN22_11760"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS43426.1"
                     /translation="MSERMIATNEQKYFPWFTTLFSLLLIVVYVSMALYSHSFSIDFG
                     LLENFGAPYAIQIYQRHVYGVVLNNLIHVNLLHLLSNLLGLWLFGAFIERRIGWFKMA
                     FFGLVSSVFGSMIQLAFTNDAGLGISSAVFGFYALILSMSFKNRLFRMKYIYALGFFL
                     FGSLVFMTITDKIFGQQVAIEAKLGGLFWGFLMGIAQSEGEI"
BASE COUNT          505 a          187 c          278 g          559 t
ORIGIN      
        1 ttaaatggtt attgattaca aatatagttt tttgaaggga atatttatga taaaagcatg
       61 ttggcacgaa aaaaaaagtt gaaaatacga tgaaagtcgg atggggaacg gaggggaaat
      121 gaaaatattt taaacaaaat gttgtttttt caaaaaaaag aactaattta gcagatagta
      181 ttcattaaat ttttacgcct atagcaaaat tttactctat ttaaatgtga agaatttata
      241 aaattcaaaa taaaatttga gtattatggc tatgaaaaat ctagatgata gcatcttgtt
      301 gagcgactac atcaatggaa acgaaaaggc gtttgaagtt ttggttctaa gacacaagga
      361 caaaatttac cgttacattt atttaaaggt taaaaatcaa ctgcttgcag aagatatttt
      421 tcaagaaacg ttcattaaag cgattcgcac tattaaatta ggagcttata atgaagatgg
      481 gaagtttttg ccttggatta tgcgtattgc ccataattta gcaatagatc attttcgtaa
      541 aaccaataaa atcaaattga tttctgaaac ttcttctaaa aacgaagact tcaatatttt
      601 caacaccttg caattgaatg aattaaacat tcaagatcaa atttctttgg atgaattgaa
      661 aacgcaaatg gtagatttgg ttgaatactt gcctgattct caaaaagata tcattaaaat
      721 gcgcatcttt caagacctat catttaaaga aattgcagat ttagaaaata taagcatcaa
      781 taccgcttta ggaagaatgc gttatgcttt aatcaatctt agaaagttga ttgaaaaaca
      841 ccaattggtt acagatattt aattttcttt tcaaatagtt tgttctaccg attgacattc
      901 caaattatga attaattttg tgtttgtcag agcgtatgat tgcaaccaac gaacagaaat
      961 attttccatg gtttacaact ttgttttcat tgttgttaat cgttgtgtat gtctcaatgg
     1021 cgttgtattc tcatagtttt tcaatagatt ttggactgtt ggaaaatttt ggagcgccct
     1081 atgctattca aatttatcaa cgccatgtat atggtgttgt tttgaataat ttaattcatg
     1141 ttaatttact acatttatta tccaatttat tagggttgtg gctatttggt gcttttattg
     1201 aacgtcgtat tggatggttc aaaatggcat tttttggttt agtaagctct gtttttgggt
     1261 caatgattca attggctttt accaacgatg caggtttggg aatttcgagt gctgtttttg
     1321 gattttatgc attgattttg agtatgtcgt ttaaaaacag actgtttcga atgaaataca
     1381 tctatgcgtt gggatttttc ttgtttggtt cacttgtttt tatgacaatt acagataaaa
     1441 tttttggaca acaagttgcc attgaagcaa agctaggtgg tttgttttgg ggctttttaa
     1501 tgggaattgc tcaaagtgaa ggtgaaatt
//
LOCUS       DAOQVQ010000544         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_113127_length_1527_cov_4.405571, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000544 DAOQVQ010000000
VERSION     DAOQVQ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113127_length_1527_cov_4.405571"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKN22_11765"
     CDS             complement(<1..438)
                     /locus_tag="PKN22_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNS43427.1"
                     /translation="MKNQNKKNMVNTIVQKIKMNRLPLLVLFGSFFIGNEALALKFNE
                     ANEGPWLLVENSDLVFMAIINLILVGVLIYFKSIFNNLLKKFAQSNTEEKSAKATGTF
                     SKILTDAVPIEEEDKITLDHEYDGIRELDNNLPPWWVALLVGTM"
     gene            complement(659..>1527)
                     /gene="ccoO"
                     /locus_tag="PKN22_11770"
     CDS             complement(659..>1527)
                     /gene="ccoO"
                     /locus_tag="PKN22_11770"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNS43428.1"
                     /translation="FRSIGGLVYLIGAVMMFYNLFKTAKQGSFEADEPAEAPALSAVL
                     AQHANEKEDWHKRLERKPVKFLIWSIIAVAIGGLVEIIPTMIVKSNVPTIASVKPYTP
                     LELEGRDIYVKEGCYNCHSQMIRPLRFETARYGDYSKAGEFVYDHPFQWGSKRTGPDL
                     AREGGLRSDYWHYQHMIRPKDVTPGSIMPAYPWLKENDLDVSLTEKKIRAMQKLGVPY
                     ETGYDLKAMGDLQKQAKQVATRIVTDIFNKLPKELQKDFNKDAEIAKMEKKEIVALIA
                     YLQRLGTDIKVK"
BASE COUNT          466 a          305 c          215 g          541 t
ORIGIN      
        1 cattgtacca accaataaag caacccacca tggtggcaaa ttattgtcta attcacgaat
       61 accatcatac tcatgatcca acgtgatttt atcttcttct tcaattggaa ctgcatctgt
      121 taagatttta gagaaagtac ctgttgcttt tgcactcttt tcttctgtat tagattgagc
      181 aaattttttc aataaattat tgaagatgct tttgaagtat attaaaactc caacaagaat
      241 caaatttatg attgccataa aaaccaaatc agagttttct acaagcagcc atggaccttc
      301 attagcttca ttaaatttca atgccaatgc ttcattccca ataaaaaagc taccaaataa
      361 taccaacaat ggtaagcgat tcatctttat cttttgaacg atagtattaa ccatattctt
      421 tttattttga tttttcatac gactaatttt gttgttgaga aaaattattt tcttcgttta
      481 tatcatcatc attgagcgga tagctactaa tttctgaaac actggattta ctcatttttc
      541 ttactctgat taaaacaatt acaaagaatg taacaaaaat caagagggaa attatcggga
      601 atattcctac tcccgatatt gtttctaaat tttgttttat aaaccgcaac atgccttatt
      661 attttacttt aatatcagta cctaaacgtt gtaaatatgc tatcaacgct acgatttctt
      721 ttttctccat ttttgcaatt tctgcatctt tgttgaaatc tttctgcaat tctttaggta
      781 acttattgaa gatatctgtt acaattcttg ttgctacttg ttttgcttgt ttttgcaaat
      841 cacccatagc tttcaagtca taacctgttt cataaggaac acctaatttt tgcatcgctc
      901 tgattttctt ctcagttaaa gaaacatcta agtcattttc tttcaaccaa ggatatgctg
      961 gcataataga acctggagtt acgtcttttg gacgaatcat gtgttggtaa tgccaataat
     1021 cacttctcaa tccaccttca cgtgccaagt ctggacccgt acgtttagaa ccccattgga
     1081 atggatggtc gtaaacaaat tcaccagctt ttgaatagtc accataacga gctgtttcaa
     1141 aacgaagtgg acgaatcatt tgtgagtgac agttgtaaca tccttctttc acataaatat
     1201 cacgaccttc taactcaagt ggtgtatatg gtttcacact tgcgattgtt ggcacattgc
     1261 tcttcacaat cattgttggg ataatttcaa ccaaaccacc aattgcaaca gcaataatac
     1321 tccaaattaa gaatttcact ggttttcttt ccaaacgttt gtgccagtct tctttttcat
     1381 tagcatgttg tgctaaaact gctgataatg caggtgcttc tgctggttca tcagcttcaa
     1441 aacttccttg tttagctgtt ttaaacaagt tgtagaacat cattacagca ccaatcaagt
     1501 acaccaatcc accaatagaa cggaaca
//
LOCUS       DAOQVQ010000545         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_113170_length_1527_cov_2.604620, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000545 DAOQVQ010000000
VERSION     DAOQVQ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113170_length_1527_cov_2.604620"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKN22_11775"
     CDS             complement(<1..481)
                     /locus_tag="PKN22_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43429.1"
                     /translation="MSTLELVAQIGVPGLGKANVASWFALGISQKLDTIEGKGWQSMS
                     YIGLGRISNPDNYAIFQKQAMLVVNQEFYHQFHHNWQYSFGLSYRLRDEYLDDYPYTH
                     DSPRQKQEFRTYVRASYTYKKGSIKLVPTLRQEFRKYYAPDFSRVSEDFQFRSRVRLQ
                     "
     gene            complement(519..968)
                     /locus_tag="PKN22_11780"
     CDS             complement(519..968)
                     /locus_tag="PKN22_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNS43430.1"
                     /translation="MIKTFLVSAFALILAANVSYGQDIKQSQVPSVVVNKFHQTFPKA
                     YDVEWELNGDLYKVEFEMGATGYDHSVWYDKSGKVVKHKEEIAKSSLPKAILEVIAKD
                     YSGYRVDDVKKYTENGKVTYKCELDSKTQDWKVTFDENGKVLNKIAD"
     gene            complement(1112..>1527)
                     /locus_tag="PKN22_11785"
     CDS             complement(1112..>1527)
                     /locus_tag="PKN22_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNS43431.1"
                     /translation="ITANETNASYQWLDCDNGMNAIVGATNQSFTATEPGNYAVAITQ
                     NGCTDTSSCVTLNDAGLTQINNTLFRVYPIPTSSTLTIVSNQNLQVDILDLLGKKVGE
                     INLVKGENKYNVQHLTTGVYYLKTSNGASHKFIKE"
BASE COUNT          466 a          294 c          244 g          523 t
ORIGIN      
        1 attgcaaacg aacacgtgat ctgaactgaa aatcttcact cacccttgaa aaatcggggg
       61 cataatattt tcgaaattct tgccgtaatg tgggcaccaa cttaattgaa ccttttttat
      121 aggtgtagga tgctcgcaca taggttctaa actcttgttt ttgtcgcggt gaatcatgcg
      181 tgtaaggata atcatctaag tattcatctc ggagacggta actcaatcca aaagaatact
      241 gccaattgtg atgaaattga tgataaaact cttggtttac caccaacata gcttgttttt
      301 ggaaaatagc gtaattatcc ggattgctaa tacgccccaa tccaatgtag gacatcgatt
      361 gccaaccttt tccttcaatg gtatctaact tttgactaat acccaaagca aaccacgaag
      421 ctacatttgc cttacctaat cctggaacac caatttgcgc aactaattca agtgttgaca
      481 aacaaaagat tgcaagacaa acgatcagtg ttttcaactt aatctgctat tttgttcaac
      541 acttttccgt tttcatcaaa agtaactttc cagtcttgtg ttttagagtc caactcacac
      601 ttgtaagtta cttttccatt ttctgtatat tttttcacat cgtcaactct gtaaccagaa
      661 taatcttttg caatgacttc taaaatggct ttgggtaaac tactttttgc aatttcttct
      721 ttatgtttta ccacttttcc agatttatca taccatacag aatgatcgta acctgttgca
      781 cccatttcaa attctacttt gtataaatcg ccattcagtt cccactctac atcataggct
      841 tttggaaatg tttgatgaaa tttattgaca accacagacg gtacttgact ttgtttgatg
      901 tcttgaccat aagaaacatt tgcagctaat atcaatgcaa atgcactaac taaaaatgtt
      961 tttatcatat tgcttgtttt tatgtattgc gaaattaagc aatcaatttg ggaaagaaat
     1021 gggaattttg aaaaaggtgg gagattctga cgataaagta aacaaaaaac ccctaaagtt
     1081 agactctagg ggttttaaaa aatactaaat tctattcttt tatgaatttg tgtgaggcgc
     1141 catttgaagt tttaagataa taaacacctg ttgtaagatg ctgaacattg tatttatttt
     1201 cacctttcac taaatttatc tcgccaactt tcttgcctaa taaatctagg atatcaactt
     1261 gtaaattttg attcgacaca attgttaagg tggaacttgt tggaattggg taaactctaa
     1321 acaaagtgtt attaatttga gttaatcctg cgtcattcaa tgtcacgcat gaactcgtat
     1381 ccgtacaccc attctgcgta attgcaactg cgtaatttcc aggttccgtg gctgtaaacg
     1441 attgatttgt tgctccaaca attgcattca taccattgtc acaatccagc cattgatacg
     1501 aagcatttgt ttcatttgct gtaatcg
//
LOCUS       DAOQVQ010000546         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_113638_length_1523_cov_3.307221, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000546 DAOQVQ010000000
VERSION     DAOQVQ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113638_length_1523_cov_3.307221"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKN22_11790"
     CDS             complement(<1..770)
                     /locus_tag="PKN22_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43432.1"
                     /translation="MKHLALLILTTIGFTAFGQKSEELIPKEAVTVLSLNNLSIFQKV
                     SIDELIQYDFMMDIQQELFDGSTSGKSIKDAGIDFNQKLNVFYGRSYDYEISGFTFGI
                     TDLNKFFEAFDDFDKEESNYPGVQYYSSFFNRLIIKGNSGLLIRVEPLEMRINDMTDS
                     LWYARGNEYYQGAYDDDYMIDNVEENILDVETEEVINEEQMFEDSVSNTEEPVFEEVK
                     DIVSGKTYWELRDSISVSMQGIYLAELLNDMFANNQNL"
     gene            936..>1523
                     /locus_tag="PKN22_11795"
     CDS             936..>1523
                     /locus_tag="PKN22_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNS43433.1"
                     /translation="MILSEEIKNYNTILFDLGGVIADLDYNNTIKAFQEKGCSNFHEI
                     YSQALQTDLFDKYETGQISSPFFINKLKELIGVDCSPNEIVAAWNAMIKGFTAEKLDF
                     LLQLKQEKTVGLLSNINDIHEEYANRKMLEITDRKLVEHFDFMFFSHHIHLRKPHPET
                     FLYVCEQMQVKPQDVLFIDDSQQHVEGAKKAGLNAI"
BASE COUNT          526 a          265 c          240 g          492 t
ORIGIN      
        1 atcaagtttt ggttatttgc aaacatatcg ttcagcaatt cagccaaata aatcccttgc
       61 atagaaacag aaatactatc tctcaattcc caataagttt ttccagaaac tatgtctttc
      121 acctcttcaa acacaggttc ttcggtattg ctaactgaat cttcaaacat ttgctcttcg
      181 ttgataactt cttccgtttc aacatccaat atgttttctt ctacgttatc aatcatgtaa
      241 tcatcatcgt aagcaccttg ataatattcg tttccacgtg cataccaaag agaatcagtc
      301 atgtcgttaa tacgcatttc caacggttcc acgcgaatca acaaaccgct gtttccttta
      361 attatcaaac gattaaaaaa ggagctataa tattgcacac ctggatagtt ggattcttct
      421 ttatcaaaat cgtcaaacgc ttcaaaaaac ttgtttaagt cggtaattcc gaaggtaaaa
      481 ccagaaatct cgtaatcata agaacgtccg taaaacacat tgagtttttg gttgaagtca
      541 attccagcat ctttgattga ttttcctgaa gttgatccat caaacaattc ttgttgaata
      601 tccatcataa aatcgtattg tatcaattca tcgatggata ctttttggaa aatagacaag
      661 ttgtttaaac tcaatacagt tacagcttct tttgggatca attcttctga tttttgacca
      721 aaagctgtaa agccgattgt ggtaagaatt agtaaagcta aatgcttcat agagattaat
      781 tttagataca aaactacata aaattagtca tgaaaatagg atattaacgt aaattaataa
      841 cactgcgcag ctaattttat tggattttat ctcaaaacag caataatctc tattgggatg
      901 atgatttttt ggcagaatat cagcatattt gcaccatgat tttaagtgaa gaaattaaga
      961 attacaatac aattctattc gatttaggag gggttattgc cgatttggat tacaacaata
     1021 ccatcaaagc atttcaagaa aaaggatgta gtaattttca tgaaatttac tcgcaagcct
     1081 tgcaaacaga tttgtttgac aaatacgaaa caggacaaat ttcaagtccg ttttttatca
     1141 ataaattgaa agaattaatt ggtgtagatt gttcacccaa cgaaattgtt gctgcttgga
     1201 acgcaatgat taaaggattt acagccgaaa aacttgattt cttattgcaa ttaaaacaag
     1261 aaaaaacggt tggtttattg agtaacatca acgatattca cgaagaatat gctaaccgca
     1321 aaatgttgga aattaccgac agaaaattgg tagaacattt tgatttcatg tttttttcac
     1381 accacattca cttgcgtaaa ccgcatccag aaacgttttt atatgtttgc gagcaaatgc
     1441 aagtgaaacc acaagatgtt ttgtttattg acgacagtca gcaacacgtg gaaggtgcta
     1501 aaaaagcagg attaaatgct att
//
LOCUS       DAOQVQ010000547         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_114003_length_1520_cov_3.892150, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000547 DAOQVQ010000000
VERSION     DAOQVQ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114003_length_1520_cov_3.892150"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKN22_11800"
     CDS             <1..496
                     /locus_tag="PKN22_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNS43434.1"
                     /translation="TEAAPIKDGVKIAFYVLDSVAETFQVYKEESERFEKEGAKLQEQ
                     LASMQRDYERIYTEYESGAKKQILTPNQMAAYEQRLGMLQQQMQEFQGSKMAAFQQKQ
                     LEATTAIQNKIIKYSEEFSKDNGIDLFLISGAGGQVAYGNPSMDVTEKFVAFMNEKEK
                     SVGK"
     gene            499..1050
                     /locus_tag="PKN22_11805"
     CDS             499..1050
                     /locus_tag="PKN22_11805"
                     /inference="COORDINATES: protein motif:HMM:NF027596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HNS43435.1"
                     /translation="MQIAEQKYQENIAEYILYMYQIEDVIRAYQFDVDAIMKNFVEPQ
                     LTDKNLAPAYKKWYSKLIRDMKDQRIEKVGHLLDLNETLIELSYLHNMLLGQANDSTY
                     IEYNEKAQSFINEFKQKSNLKEKNDVELIFGAMYMKLLLKLQKKEISPATESAFDAMR
                     MVLAYLAKSYHKMKNGDLDFLNN"
     gene            1069..>1520
                     /locus_tag="PKN22_11810"
     CDS             1069..>1520
                     /locus_tag="PKN22_11810"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNS43436.1"
                     /translation="MNEITIKYQEFESFDQLSDEDLDLIDRAFIAASNAYAPYSNFQV
                     GASVLLNDGTIVLGNNQENSAYPSGICAERTALFYAGANFPQHSIKKMAIVAFGDAFS
                     EETFLSPCGSCRQVMLESGNRQDHPFEILLTSPNKKVIKLFSAKDLLP"
BASE COUNT          553 a          248 c          286 g          433 t
ORIGIN      
        1 aacagaggca gctccaatta aagatggtgt taaaattgca ttttatgtat tggattctgt
       61 tgcagaaacg tttcaagtgt acaaagaaga atcagaacgt tttgaaaaag aaggtgctaa
      121 attgcaagaa caattggcat ctatgcaacg tgattacgag cgtatctata ctgaatatga
      181 aagtggcgct aaaaaacaaa tattgacacc caatcaaatg gctgcgtatg aacaacgttt
      241 aggaatgttg caacagcaaa tgcaagaatt tcaaggctca aaaatggctg cttttcaaca
      301 aaaacaattg gaagcaacta cagctattca aaacaaaatc attaaataca gcgaagagtt
      361 ttctaaagat aatggcattg atttgttctt aatcagtggg gcgggcggac aagtagctta
      421 cggaaatcca tccatggatg taaccgaaaa atttgttgcc tttatgaacg aaaaagaaaa
      481 atctgtaggt aaataaacat gcaaatagcc gagcaaaaat accaagaaaa tattgcagaa
      541 tatattttgt atatgtacca aattgaagac gttattcgtg cctatcaatt tgatgtagat
      601 gcaattatga aaaattttgt agagccacaa ttgaccgaca aaaatttagc acctgcctat
      661 aaaaaatggt attctaaact cattcgcgat atgaaagatc aacgcattga aaaagtggga
      721 catcttttgg acttgaatga aactctcatc gaattgagtt atttacacaa catgttattg
      781 gggcaagcaa acgactctac atacattgaa tacaatgaaa aggcacaatc gtttatcaat
      841 gaattcaagc aaaaatcgaa tttaaaagaa aaaaacgatg tggaattaat ctttggagca
      901 atgtatatga aattgttgct caaactccaa aaaaaggaaa tttcgccagc aaccgaaagc
      961 gcttttgatg caatgcgtat ggtgcttgcc tatttggcaa aatcctacca taaaatgaaa
     1021 aatggtgatt tggatttttt aaataattaa tcttaaaacc tactagctat gaacgaaatt
     1081 acaattaaat accaagaatt tgaatcgttt gaccaattgt cggatgaaga tttagattta
     1141 attgatagag ctttcatagc agcatcaaac gcctatgcac cctattccaa ctttcaagtg
     1201 ggggcaagtg tgcttttaaa tgatggaact attgttttag gaaataatca agaaaatagt
     1261 gcttatccgt ctggaatctg tgccgaacga actgctttgt tttatgcagg agcaaatttt
     1321 cctcaacatt ccattaaaaa aatggccatt gttgcctttg gcgatgcttt ttcagaagaa
     1381 acgtttcttt ccccttgcgg tagttgccgc caagtgatgt tggaatcggg aaatagacaa
     1441 gatcatccct ttgaaatttt gctgacaagt cccaataaaa aagtgataaa gctattttcg
     1501 gcaaaggatt tgttaccctt
//
LOCUS       DAOQVQ010000548         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_114118_length_1519_cov_3.190574, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000548 DAOQVQ010000000
VERSION     DAOQVQ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114118_length_1519_cov_3.190574"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKN22_11815"
     CDS             complement(<1..526)
                     /locus_tag="PKN22_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43437.1"
                     /translation="MKPSNELFKLVKSLSKSEKRFFKLSSSLQAGEKNYIKIFDFIDA
                     QKEYDEEALKEEFKNEVFIKHLSSEKNYLYKLILKSLRNFYSEDSPGSILKLEIKNIE
                     ILFSKALYKECEKYIQRVKELAKSTEDFYYWNEIISWEKWLIEDGLQETLNEVLLEEL
                     IKEEIEVVEKLRNLA"
     gene            737..>1519
                     /locus_tag="PKN22_11820"
     CDS             737..>1519
                     /locus_tag="PKN22_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS43438.1"
                     /translation="MTSFFSDFFKQFAHGFELPLQNLILVFSVILLIILLSPILLKKL
                     NIPGIIGLIIAGVIIGPHGFNVIEKNSAIDLFSLIGLLYIMFIAGLELDMNEFMVNKN
                     KSLVFGFFTFIIPLSVGFPVVHYFLGYDFDTSFLTASMFATHTLVAYPIVSKLGVSKN
                     KAVPVAVGGTILTDTAVLIILAVIIGKAHGSLNAAFWIQLLVSLAIFSAIMFLVIPRI
                     AAWFFRKLESEKHSHYIFVLSVVFFAAFLAEVAGVEPIIGAFV"
BASE COUNT          422 a          266 c          233 g          598 t
ORIGIN      
        1 cggctaaatt tcggagtttc tctacaactt caatttcttc ttttatcaat tcttccaata
       61 aaacttcatt taaagtttct tgaagtccgt cttcaattaa ccatttctcc cacgagataa
      121 tttcattcca gtaatagaaa tcttctgtgc tttttgccaa ctcttttaca cgttgaatgt
      181 atttttcaca ttctttatat agtgctttac taaaaagaat ttcaatattt tttatttcaa
      241 gctttagaat actacctggc gagtcttcac tatagaaatt acgaagactt ttgagaatca
      301 atttatacaa atagtttttc tccgacgata aatgtttgat aaacacttcg tttttaaact
      361 cttctttgag cgcttcttca tcgtattctt tttgcgcatc gatgaagtca aaaattttaa
      421 tgtaattttt ttctcctgct tgtaatgaag atgatagttt aaagaatcgt ttttcagatt
      481 ttgacaacga tttgactagc ttaaacaatt catttgaagg tttcattgag tgtgtgctat
      541 ttgaaaattc actacaaaaa tacaatttat ctttgaaatc ctaaaattac ttttttataa
      601 ctatttacag ccttaataag ctattcagat gaatacatca acttcttcaa ctaccttcaa
      661 aactgttaat aaatcccgat taaattctca ataatattta ttatttttgt tttcctaata
      721 attattacag ttcaagatga cttcattttt ttccgatttc tttaaacaat ttgcacacgg
      781 ttttgaattg cctttacaaa atttgattct tgtattttca gtcatacttc tcatcatctt
      841 gttatcgccc attttattaa aaaagctcaa cattcctgga attattgggt taatcattgc
      901 tggggtaatt attggacccc atggtttcaa cgtgattgag aaaaactctg ccatcgattt
      961 attttcactc attggcttgc tctatattat gtttattgct ggtttagagc tggatatgaa
     1021 tgaatttatg gtcaacaaaa acaaaagttt ggtatttgga tttttcacct ttatcattcc
     1081 actttctgtt ggcttccctg tagtacacta ctttttaggc tatgactttg acacaagttt
     1141 tttaaccgcg agtatgtttg ctacgcatac acttgtggcc tacccaattg tgagtaagct
     1201 gggggtttct aaaaacaagg ccgtgcctgt tgctgttgga ggaaccatct taacggacac
     1261 cgccgttttg attattcttg cagttatcat tggaaaagca cacggaagtt taaatgctgc
     1321 attttggata caattattgg tgtctttagc aatattttca gccatcatgt ttttagtgat
     1381 accacgcatt gctgcttggt tttttagaaa attggagagt gaaaaacatt cgcactacat
     1441 ttttgtattg tcggttgtgt tttttgcggc ctttcttgca gaagtggcgg gtgtagaacc
     1501 aattatcggt gcattcgtt
//
LOCUS       DAOQVQ010000549         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_114558_length_1515_cov_4.937671, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000549 DAOQVQ010000000
VERSION     DAOQVQ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114558_length_1515_cov_4.937671"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="PKN22_11825"
     CDS             complement(<1..1457)
                     /locus_tag="PKN22_11825"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS43439.1"
                     /translation="MKQILFFAFSCILFTHSFAQTATVKGIVTDAETTETMIGALVQF
                     TTELKAKTDFDGSYTIENVPYGEYSVQVSMPTYKTKTFKVTVNKEITTFDFVLGEIAV
                     LEEVQVTANIVQDRKTPVAVSNLDKKQIIEELGSQDLPMVLNSKPGVHATQQGGGDGD
                     ARITIRGFDQRNVGVMIDGVPVNDMENGWVYWSNWFGLDQITQQIQVQRGLGATKLAM
                     PSVGGTMNVITENAFGNAGGKIQQEYGSGNFFRTSFSYRSGMLKNGWGFVMSASYKQG
                     NGWVDGLNTQGGFYYLKIQKKLKNHVLSLSAFGAPQQHAQRSYNQKITYWSAKYANKL
                     GIDTTGALDHGIRYNQHYGYYTDENGKQKIMAARRNYYHKPQVTLRDFWKINEKVSWT
                     NTAYVSIGRGGGEREKNSGGIIYKEDGTIAWDTIIRNNQEVTFFGSKYPSIDPLYSTT
                     ELKSSQVLVSMVNNHIWAGGVSQVDYKINSDWNFAG"
BASE COUNT          433 a          310 c          262 g          510 t
ORIGIN      
        1 cctgcaaaat tccaatcaga gttgatttta taatctactt gcgatacacc tccagcccaa
       61 atatggttgt taaccataga aaccaacact tgagaagatt ttaactcagt agtgctatac
      121 aatgggtcga ttgaagggta tttagatcca aaaaaagtaa cctcttggtt gtttctaata
      181 atcgtatccc aagcaatggt tccatcttct ttgtaaatga ttccacctga gtttttctca
      241 cgctcacctc caccacgacc tatggaaaca tacgcagtgt ttgtccatga aactttttcg
      301 tttattttcc aaaaatcacg caatgttact tgtggtttgt ggtaataatt tcttctggca
      361 gccataattt tttgcttgcc gttttcatcc gtatagtaac cataatgctg gttgtaacgg
      421 ataccatgat ccaatgcacc tgttgtgtcg atgcctaatt tgttggcata ttttgcactc
      481 cagtaagtta ttttttggtt gtaggaacgt tgtgcatgct gttgaggtgc accaaaagca
      541 ctcaatgaaa gcacatggtt ttttaatttc ttttgtattt ttaagtagta gaatccacct
      601 tgtgtgttta atccatccac ccatccgttt ccttgcttat aggatgcaga cattacaaat
      661 ccccatccat ttttaagcat tccagaacgg taagagaaag atgttctaaa gaagtttcca
      721 gagccatatt cttgttgaat tttaccacca gcattaccaa atgcattttc agtaataacg
      781 ttcattgtac caccaactga tggcatggct aattttgtag cacccaaacc acgttgaact
      841 tgtatttgtt gtgtaatttg atccaaacca aaccagtttg accaatatac ccaaccgttt
      901 tccatatcgt ttacaggaac accatctatc attacaccta cgttgcgttg gtcaaaacca
      961 cgaatggtaa ttcgagcatc gccatcgcca ccaccttgtt gtgtagcgtg aacacctggt
     1021 tttgagttga gcaccattgg taaatcttgc gaaccaagct cttcaatgat ttgtttttta
     1081 tcaagattgg ataccgcaac cggtgttttt ctatcttgca caatattggc agtaacttgc
     1141 acttcttcca atactgcaat ttctcccaac acaaagtcaa aagtggtaat ttctttgttt
     1201 acagttactt taaaagtttt ggttttgtat gttggcattg aaacttgtac cgaatattcg
     1261 ccataaggca cgttttcaat tgtataagaa ccatcaaaat cggttttggc ttttaactca
     1321 gtggtgaatt gtaccaatgc accaatcatg gtttctgtcg tttcggcatc ggttacaata
     1381 cctttaactg ttgcagtttg agcaaatgaa tgtgtaaaaa gtatgcaact gaaagcaaaa
     1441 aataggattt gtttcataaa ttaaaactaa aaaagccctc tgatgtgttt accagagggc
     1501 taataaattt acttg
//
LOCUS       DAOQVQ010000550         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_115211_length_1510_cov_3.650172, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000550 DAOQVQ010000000
VERSION     DAOQVQ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115211_length_1510_cov_3.650172"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PKN22_11830"
     CDS             <1..1178
                     /locus_tag="PKN22_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688794.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNS43440.1"
                     /translation="YDEGFVGYGLKKAEYISDCSEIDDVIIFFASGKMMVSKVSEKKF
                     VGKGIVYANVWKKGDKRTIYHMIYQDGIGGATMMKRFTVDSITRDTEYDLTRGVKGSK
                     MLYFSVHPNGEREIVTVMLRPRPHIKRLRFDIDFGALLIKGRSAAGNRVTKEIVQKIV
                     QKEVGGSTLAARKIWYDTIVGRLNDEGRGTFLGQFKGNDKILTLYKNGEYRLSSFDLA
                     THFDEDMMHIEKWHPERPISAVYYDAEKDMHFVKRFLCEVTMDKRVSFISESEGSLLD
                     IATTAYRPKARVVYNKLLKATKNLPDTIIELADFIEVKGMKAQGNQLTKLKVKEVLIE
                     IPEGGEEPWPEANNDVAASTTENENLELDNDSNDGEAPTMEWDLTKDEDDNQPSLFE"
     gene            complement(1181..>1510)
                     /locus_tag="PKN22_11835"
     CDS             complement(1181..>1510)
                     /locus_tag="PKN22_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43441.1"
                     /translation="ILFTQDVVYNPKKLPFEIALRYAMFDTDSYDSRIYSFEANALYV
                     YSIPAYYYQGSRAYLLFRVRVFKNLDIWARYGLFLYNNRKTLGSGPEEIKGNHKQDLT
                     LQLRLKF"
BASE COUNT          508 a          246 c          332 g          424 t
ORIGIN      
        1 attacgacga aggatttgtg ggttatggct tgaaaaaagc agaatacatc tcggattgtt
       61 ctgaaattga cgatgtgatt atcttctttg cttcgggtaa aatgatggtt tcaaaagtca
      121 gtgaaaagaa atttgtaggc aaaggaattg tgtatgccaa tgtttggaaa aaaggcgaca
      181 aacgcaccat ttatcacatg atttaccaag atggaattgg tggagctacc atgatgaaac
      241 gctttacagt tgattccata acacgtgata cagaatatga cttgacacgt ggtgttaaag
      301 gttccaaaat gttgtatttc tctgtgcatc caaatggaga aagagaaatt gtaaccgtta
      361 tgttgcgccc acgaccacac atcaaacgtt tgcgttttga tattgacttt ggcgcattgc
      421 tcattaaagg tagaagtgct gcgggtaacc gtgtaacaaa agaaattgtt caaaaaatcg
      481 ttcaaaaaga agtgggaggt tctacccttg cggctcgtaa gatttggtac gacacaattg
      541 ttggtcgatt aaacgatgaa gggcgtggaa cattcttggg gcaattcaaa ggcaacgata
      601 aaattctaac cttgtataaa aatggtgagt accgtttgtc tagttttgac ttggcaaccc
      661 attttgacga ggatatgatg cacatcgaaa agtggcatcc agaacgtccc atttcagcag
      721 tttattacga tgccgaaaaa gatatgcatt ttgttaaacg tttcttgtgc gaagtaacga
      781 tggacaagcg tgtttcattc atcagcgaaa gtgaaggttc gttgttggat atcgcaacca
      841 cggcatatcg tccaaaagca cgtgttgttt acaataaatt gttaaaagca actaaaaatc
      901 ttccagacac catcattgaa ttagctgatt ttatagaagt taagggaatg aaagcgcaag
      961 gaaaccaatt gacaaaatta aaagttaaag aggtgttgat tgaaattcct gaaggtggcg
     1021 aagaaccgtg gccagaagcc aataatgatg ttgctgcatc aacaacagaa aatgaaaatt
     1081 tggaattgga taacgacagt aatgacggtg aagctccaac aatggaatgg gatttgacaa
     1141 aagacgagga cgataatcaa ccaagtttgt ttgaataaac ctagaatttt aaacgcaatt
     1201 gtagggttaa atcttgtttg tgattgcctt taatttcttc gggtccagaa cctaaagttt
     1261 tgcgattgtt gtacaaaaac aacccgtatc ttgcccaaat gtcaaggttt ttgaatacac
     1321 gcactctaaa caacaagtag gcgcgacttc cttggtaata gtaagctgga attgaataca
     1381 cataaagtgc atttgcttca aacgagtaaa ttctagagtc gtaactatcg gtatcaaaca
     1441 tggcatagcg caaagctatt tcaaaaggta attttttagg attatagaca acatcttggg
     1501 taaacaaaat
//
LOCUS       DAOQVQ010000551         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_116642_length_1499_cov_3.903047, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000551 DAOQVQ010000000
VERSION     DAOQVQ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116642_length_1499_cov_3.903047"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKN22_11840"
     CDS             complement(<1..493)
                     /locus_tag="PKN22_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNS43442.1"
                     /translation="MANLTQTQGIAGFDWEALELDGYNKIQRSELSDKYGQTLTSINE
                     REVVEGTVVAITKREVIVNIGYKSEGVIPSSEFRYNPELKVGDKVDIYVEVQEDKSGQ
                     LVVSHRTARMHKAWIRVNDVLRSGEIITGYVKCRTKGGLIVDVFGIEAFLPGSQIDVK
                     PIRD"
     gene            709..>1499
                     /locus_tag="PKN22_11845"
     CDS             709..>1499
                     /locus_tag="PKN22_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNS43443.1"
                     /translation="MDKKSWFTSLGLYFVLYFVILTLIFTTSTPNQFDGLSALRFAGI
                     LFLFFVSYKRKKLSLWIFTSMLLGIEVGITFPAIGLEMERLGTIFLRLVKTLVAPLII
                     ATLIVGIAGHSNLKQLGRIGLKSILYFEVVTTFALVIGLLAINLTKAGEGVSVEARQA
                     DLAKSEVLMQQTTSDHQRDHLVEIFPENIAKSIAGNEILQVVVFSIIFAIAIGMIEKA
                     KPKETMLNFFEGLSEVMFKFTDIVMYFAPFAVFGAIAATVAKSGL"
BASE COUNT          435 a          259 c          270 g          535 t
ORIGIN      
        1 aatcacggat aggtttaaca tcgatttgag aacctggtaa gaatgcttcg ataccaaata
       61 cgtcaacaat taaaccacct tttgtacggc atttaacata acctgtaata atttctcctg
      121 aacgtaatac atcattaaca cgaatccatg ctttgtgcat acgagcagtt ctgtgagaaa
      181 ctactaattg tcctgatttg tcttcttgaa cttcaacgta aatgtctact ttgtcaccaa
      241 cttttaattc aggattgtaa cggaattcag atgatgggat aacaccttca gatttgtaac
      301 cgatgttaac aattacttct cttttagtaa ttgcaacaac tgtaccttca actacttctc
      361 tttcgttgat agaagttaaa gtttgtccgt acttgtctga taattctgaa cgttggatct
      421 tgttgtatcc atccaattct aaagcttccc aatcgaaacc tgcaattcct tgcgtttgtg
      481 taagatttgc catttggcta ttaaaatttg tatttcggaa accctccatt ccgaaagtgt
      541 ttaacatatc atttgaggga aatgatttat tttgagggcg caaatatacg acatttcttt
      601 ttaaatacaa aaaaatcaaa gttttatgtg cttaatttgc tattttatgg tggatttaca
      661 ttgtcaattc acttaatttt atcactttta ccgcttaaat ctgtatcaat ggacaaaaaa
      721 agttggttta cttccttagg cttgtatttt gtactctatt ttgttattct aacattaatc
      781 tttactacct caacacccaa tcaatttgat ggtttgtcgg ctttacgatt tgctggaata
      841 ctatttttat tttttgtttc ttataaacgc aaaaaattgt cgttgtggat ttttacatcc
      901 atgttacttg gaatagaggt cgggattact ttccctgcaa ttggtttgga aatggaacgc
      961 ttgggcacca ttttcttgcg ccttgttaaa acattggttg caccacttat aattgccact
     1021 ctaattgttg gaattgctgg acatagtaat ttaaaacaat tgggacgcat tgggttaaaa
     1081 agtattcttt attttgaagt ggtaaccact tttgcattgg tcataggatt gttggccatt
     1141 aatcttacta aggctggaga aggtgtttcg gtggaagcac gacaagcaga cttggcaaaa
     1201 tcagaagtat tgatgcaaca aacaacatcg gatcatcagc gcgatcattt agtggaaatt
     1261 tttccagaaa acattgcaaa atcaattgct ggaaatgaaa ttctgcaagt tgttgtgttt
     1321 tccatcattt ttgccattgc aattgggatg attgaaaaag caaaacctaa agaaaccatg
     1381 ttgaactttt ttgagggatt gagcgaggtg atgtttaaat ttacggatat cgtgatgtat
     1441 tttgcaccat ttgcagtttt tggtgcaatt gccgctacgg ttgccaaatc tggactcga
//
LOCUS       DAOQVQ010000552         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_116906_length_1497_cov_3.570735, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000552 DAOQVQ010000000
VERSION     DAOQVQ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116906_length_1497_cov_3.570735"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1479
                     /locus_tag="PKN22_11850"
     CDS             196..1479
                     /locus_tag="PKN22_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS43444.1"
                     /translation="MSGETDVKVVKVSQLADGIIGSEIIKLASEVNEKIKQGEKIYNL
                     TIGDFNPKHFPIPQELKQYIVDEYNADATNYPEANGMLALRQAVSNFLKVKGNLDYTT
                     DEILISGGARPIIYSIFRTIVDANDTVVFAVPSWNNNHYSYLNNANAVVIEADAATNF
                     MPSAADIQPYIGEASLVALCSPQNPTGTVFTKEGLEEICDLILAENKKREGVRKPVYL
                     MYDQIYWALTHGDTEHQNPVNLRPEMKEYTIFVDGVSKSLSATGIRVGWTMGPKFVID
                     KMKAILTHIGAWAPKAEQLAVAKYISNIEMYNEFLAVQKDKIYTRLKGFYDGFQALKA
                     EGHNVDAIAPQAAIYLTVNIDLKGKRTADGKLIETTQDITSYILNEAKIAIVPFYAFG
                     ASKTSTWYRMSVGTCGVDDIAGVINNLRAALNKLS"
BASE COUNT          520 a          255 c          282 g          440 t
ORIGIN      
        1 atttaacttg atttttcact attttttaaa aaaggcaaat ctaacaaatt tcttttattt
       61 tcaaggatat tttttgttta acattaagaa tgctaaggaa aatgccagtt taaaaaatta
      121 acaacatttt gatttgaaca caattttatc cgatagatta tagttatttt tgcagcaaat
      181 caaattttaa agattatgag tggagaaaca gatgttaaag tggttaaagt ttcacagtta
      241 gcagacggaa tcattggttc tgaaatcatt aaattggctt cagaagtaaa cgaaaaaatc
      301 aaacaaggag aaaaaatata caatttaaca ataggcgatt tcaaccctaa gcacttccct
      361 attccacaag aattaaagca atacattgtt gatgaataca atgcagatgc aaccaactat
      421 ccagaagcaa atggtatgtt ggcattacgt caggcagttt caaacttctt aaaagtgaaa
      481 ggaaatttgg actacacaac cgatgaaatt ttgatttctg gtggtgcacg tccaattatt
      541 tactctattt tcagaaccat cgttgatgca aatgatacgg ttgtttttgc cgttccatcg
      601 tggaataaca accattattc ttacttaaac aacgctaatg cagttgtaat tgaagcggat
      661 gctgcaacga atttcatgcc ttcagctgcc gatattcaac cttacattgg tgaagcgagt
      721 ttagtggcgc tttgttcacc tcaaaaccct actggtacgg tgtttacaaa agaaggattg
      781 gaagaaattt gcgatttaat tttggctgaa aacaaaaaac gcgaaggtgt tagaaaacca
      841 gtttatttaa tgtacgatca aatttattgg gcattaacac acggtgacac ggaacaccaa
      901 aatccggtaa acttgcgccc agaaatgaaa gaatatacca tttttgtgga tggtgtttct
      961 aaatcgttat cggcaacggg gattcgtgtt ggttggacca tgggaccaaa atttgttatc
     1021 gacaaaatga aagctatttt gacacacatt ggggcgtggg caccaaaagc agagcaattg
     1081 gcagtggcaa aatacatcag caacattgaa atgtacaatg aatttttagc ggttcaaaaa
     1141 gacaaaattt acactcgctt aaaaggattt tacgatggat tccaagcatt gaaagccgaa
     1201 ggtcacaatg ttgatgcaat tgcaccgcaa gcagccattt atttaaccgt aaacattgat
     1261 ttaaaaggca agcgcacagc agatggcaag ctaattgaaa caacacaaga catcacctct
     1321 tacattttaa acgaagcaaa aattgccatc gttccattct atgcatttgg cgcttcaaaa
     1381 acttccactt ggtacagaat gtcggttgga acatgtggtg ttgacgatat tgctggcgta
     1441 atcaacaact tacgtgctgc attgaataaa ttgagctaag aaataaggct tttttat
//
LOCUS       DAOQVQ010000553         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_116946_length_1497_cov_2.558946, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000553 DAOQVQ010000000
VERSION     DAOQVQ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116946_length_1497_cov_2.558946"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1497)
                     /locus_tag="PKN22_11855"
     CDS             complement(128..>1497)
                     /locus_tag="PKN22_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNS43445.1"
                     /translation="HWSNKGIAYHGNPIDGLQVMGILETRLLDFKNIICLGMNEGMMP
                     PTNPIQSLFPMDLRRYVGLPLPRDKQGLFAHHFYRLLHQCENLLVTYSGTKESVGSNE
                     KSRYLQQIEKELVRNNPAIDWQFSYYNVPMEDDKLAEFTAIQKDAAIFKRMDELFERS
                     TSFSTLSKYVQCSLDFYYRYVLEFGEEDGVEEDLESNTFGTILHSTLERLYTKYAKFN
                     ADGSVNFQGGKPLQKEAILEMKLLSNNYLEEEFLKYFNNDKQSFTYGMNRLSYVIAQK
                     MVAEFLDKELAFIEKGNSVIIHSLEKKISLPLQLEINGEQKTINLNGTIDRIDEVNGH
                     IRLIDYKSGKSDDKKRTYATSKEKEDWQVNFKNATHFTQLLMYCYMYYRTYGVMPTRA
                     GIFSMVNIKGGLLEMDTKGNSIESLVDAFPEWLTGIFSDMYAASKPFEHTSGDYNNYC
                     SYCKK"
BASE COUNT          440 a          292 c          251 g          514 t
ORIGIN      
        1 cagcacactc ataagtattc ttttattcat cttgaaaaat ttaaattcaa aagcgaaatt
       61 aagacttttt taacaagcga caaaatatat cgcaatatat ttatcaacat tcacaaaaaa
      121 cttataatta ttttttacaa taagaacagt agttattgta atcgccagaa gtgtgttcaa
      181 aaggttttga agctgcatac atgtctgaaa aaatgcctgt gagccactct ggaaatgcgt
      241 caactaagga ttcgatgctg ttacctttgg tatccatttc caataaaccg ccttttatgt
      301 tgaccattga aaaaatacct gcacgtgttg gcattacgcc ataagtgcgg taatacatgt
      361 agcagtacat caacaattgt gtgaaatggg tggcattttt gaaattaact tgccaatctt
      421 ctttctcttt tgaagtggca taggttctct ttttatcgtc ggatttgccc gatttataat
      481 ctatcaaacg gatgtgacca ttcacctcgt ctattctgtc aatggtaccg ttaaggttga
      541 tggttttttg ttcgccattt atctccagtt gcaagggaag acttattttt ttctctaaac
      601 tgtgaatgat gactgaattt cctttttcaa taaatgctaa ttctttatct aaaaattcag
      661 ctaccatttt ttgagcaatg acataggata ggcggttcat tccgtaggta aatgattgtt
      721 tgtcgttgtt gaagtatttt aaaaactctt cttccaaata gttgttcgac aaaagtttca
      781 tttctaaaat tgcttctttt tgcagcggtt ttcctccttg aaaattcacc gatccatctg
      841 cattgaattt agcatatttg gtgtataaac gctccaaggt gctgtgtaaa atcgttccaa
      901 aggtgttgct ttctaagtcc tcttctactc catcttcttc gccaaattct aatacataac
      961 ggtaatagaa atccaacgaa cattgcacgt atttactcaa tgtagaaaat gaagttgaac
     1021 gttcaaacaa ctcgtccatt cgcttaaaaa ttgcagcatc tttttgaata gctgtaaatt
     1081 cggccaattt atcgtcctcc atcggcacgt tgtaatagct aaattgccaa tcaattgctg
     1141 gattattgcg caccaattct ttttctattt gttgtaaata gcgacttttc tcattgctcc
     1201 ccaccgattc ttttgtaccc gaataagtca ccaacaaatt ttcgcattgg tgcaataaac
     1261 gataaaaatg gtgagcaaac aatccttgtt tatcgcgtgg tagaggcaaa ccaacgtagc
     1321 gacgcaaatc catcgggaaa agtgactgaa ttggattggt tggcggcatc attccttcgt
     1381 tcattcctaa gcaaatgatg tttttgaaat ccaacaaacg tgtttccaaa attcccatta
     1441 cttgcaaacc gtcaataggg tttccatgat aggcaattcc tttgttgctc caatgtt
//
LOCUS       DAOQVQ010000554         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_117040_length_1496_cov_3.285913, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000554 DAOQVQ010000000
VERSION     DAOQVQ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117040_length_1496_cov_3.285913"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..>1496
                     /locus_tag="PKN22_11860"
     CDS             236..>1496
                     /locus_tag="PKN22_11860"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HNS43446.1"
                     /translation="MADNILEYVLSLKDKVSANLQIIGVNNDTALDKFSKLELQASKV
                     SREMTDLGKNAYTLGQKLALLKNERDLIDASDITSIKKYNSEIKQLETEISNIQNATG
                     KGRIAGWFSDAFQSIPFAGLLTNPLVVAGSVAGKALSVGMEADLNKTAFSVMLNSDDA
                     ADQLITSLKGIKMDQGALGESAKTMLGFGVATEKVVPMLDAIGNIAGGDSAKVSSLSL
                     AFSQMSSTGKLMGQDLLQMINAGFNPLAEIARTTGRDLGELKDEMAKGNITVEMVEEA
                     FKSATGEGGSFNGMLDKMNNTLSGKIQNAMMKVNELLLQLYTVLEPIVSFSLDVLVGL
                     FNAVTESVDWFVNGLSELNPLATTLGIIIGSLITGMAAYIVKTKAQTTWSKIQVAGQK
                     LQVLWTNLTSAAWWKLNAAMLANPIVWI"
BASE COUNT          505 a          232 c          322 g          437 t
ORIGIN      
        1 tggtgtggga atgaagtaga gctaattagt cgccacttct agccatattc ctaacagcaa
       61 aataaggcta aatagaataa tagtaaacat catgctgatg aataacactt tattattctt
      121 tttcgcatct ttcaaaacag cagggacacc aacaagtgca aacaaagata ttttgaatac
      181 aactttcaag atgaaataaa gaaaaaacgt tccaaacagt atataaagaa tcaccatggc
      241 agacaatatt ttagagtatg tgcttagtct taaagacaaa gttagtgcta atttacaaat
      301 aattggcgtt aacaacgata ctgctttgga taaattttca aaattagagc tacaagcttc
      361 taaagtaagt cgtgagatga ctgatttagg taagaacgct tatactttag gtcaaaaatt
      421 agccctctta aaaaacgaac gtgatttaat tgatgcaagc gacattacct cgattaaaaa
      481 atacaactca gaaataaaac aattagaaac tgaaatttct aacattcaaa acgcaacagg
      541 taagggaaga atagctgggt ggttttctga tgcttttcaa tcaattccat ttgctggact
      601 gcttaccaat ccgttggtgg tagcgggttc agtggcaggc aaagctttat ctgttggtat
      661 ggaagctgat ttgaataaaa cagctttttc ggttatgctt aattcggatg atgctgccga
      721 tcagttaatt acctctttga aaggaattaa aatggatcaa ggcgcattgg gtgagtcagc
      781 caaaactatg cttggatttg gtgtggcaac cgaaaaggtt gtaccaatgc tagatgcaat
      841 aggtaacatt gccggaggtg attccgctaa agtatcttca ttatcgcttg ctttttcaca
      901 aatgtcttcc actggtaagc tgatggggca agatttgcta cagatgatta atgctggttt
      961 taatccattg gcagaaatag ctagaacaac ggggagagat ctaggtgagt taaaagatga
     1021 aatggctaag ggtaatatta ccgtagaaat ggttgaagaa gcttttaaat ccgcaactgg
     1081 tgagggaggt tctttcaatg gtatgttgga taaaatgaac aatacattga gtggtaaaat
     1141 tcaaaacgcc atgatgaaag ttaacgaatt actgttgcaa ttatacaccg tattagagcc
     1201 aatcgtatcc ttttcattag atgtgttggt tggattgttt aatgctgtaa ctgaaagtgt
     1261 agactggttt gtgaacgggt tgagtgaatt gaatcctttg gctacaactc ttggaataat
     1321 aataggttcg ttgattacag gaatggccgc atatatagtt aaaacaaagg cacaaacgac
     1381 gtggtccaaa atacaagttg cagggcaaaa attacaagta ctttggacga atttaacatc
     1441 tgcggcttgg tggaaattaa acgcagctat gttagccaac cctattgtgt ggattg
//
LOCUS       DAOQVQ010000555         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_118548_length_1485_cov_2.210490, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000555 DAOQVQ010000000
VERSION     DAOQVQ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118548_length_1485_cov_2.210490"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..942
                     /locus_tag="PKN22_11865"
                     /pseudo
     CDS             8..942
                     /locus_tag="PKN22_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
     gene            1036..>1485
                     /gene="prs"
                     /locus_tag="PKN22_11870"
     CDS             1036..>1485
                     /gene="prs"
                     /locus_tag="PKN22_11870"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNS43447.1"
                     /translation="MSAGIKIFAGRASEELAGKIAKAYGVSLGDVKVTDFSDGEFQPS
                     FEETVRGMDVFIVQSTMPPTDNLFELLLMIDAAKRASARKIIAVCPYFGFARQDRKDK
                     PRVAIGAKLVANMLMAAGVDRIMTMDLHADQIQGFFEVPVDHLFASTI"
BASE COUNT          438 a          247 c          328 g          472 t
ORIGIN      
        1 aaaaatcatg agtaaagcat tgatttaccc aggaagacat accatcaacg ttattttgct
       61 tttagtgacc attgttttgg gtgttttatt tgcacaatct catggaactg atggtatgat
      121 ttatttgtac atcatgactg ctattggctg ttttattggt gttatgatgg taatggcaat
      181 tggtggtggc gatatgccag tggttgtttc tatgttgaac tcctactctg gatgggctgc
      241 tgcagctgcc ggttttatgt taggaaacga tttattgatt gttacaggag ccttggtagg
      301 tagttcaggt gctattcttt ctatccacat gagtaaagca atgaaccgta aatttatctc
      361 tgttattttg ggtggatttg gcacagcaag tagttcaaca aaagaaattg aaggtgagat
      421 cacaaccatt aaccacgaag aagctgccga attgttgcgc caagcaaaat ctgttgtgat
      481 tgttccagga tatggtatgg cggttgcaaa ggctcaatat ccaattcacg atttagtgca
      541 aacattgcgc aaagaaggaa aaacagtgcg ttttgctatt cacccagtag ctggacgttt
      601 gcctggacac atgaacgtat tgttagcaga agcgaaagtt ccatacgata ttgttttgga
      661 atggatgaaa tcaatccaga tttaccagac acagatgttg taatggtaat tggtgcaaac
      721 gacgttgtaa atccaagtgc acaagacgat ccaggatgtt ctatttatgg aatgccagtt
      781 attgaagctt ggaaagcaga aaaagttatc atcatgaagc gttcactttc ggcaggttat
      841 tcgggagaag acaatccgtt gttctaccgt gaaaactctg aaatgctatt gggcgatgcc
      901 aaagacagca ccgataagtt gctctcttat ttgaaaaaat aaaaaaatta aacgggttct
      961 aaaaagaatc cgtttttttt tattctaatt aacaaaaaaa ccataacttt gcatcctcat
     1021 tttgagcaat aaattatgtc agcaggtatt aaaatttttg caggaagagc ttccgaagag
     1081 ttagcaggta aaatcgctaa ggcttatggt gtttctttag gcgacgtgaa agttactgat
     1141 tttagtgatg gtgaatttca accgtcattt gaagaaacag tacgtggtat ggatgttttc
     1201 atcgttcaat caacaatgcc tcctactgac aatttatttg aattattgtt gatgattgat
     1261 gccgcaaaaa gagcttctgc acgcaaaatc atcgctgttt gtccttactt tggttttgct
     1321 cgtcaggata gaaaagacaa accaagagtt gcaatcggtg ccaaattagt tgccaacatg
     1381 ttgatggctg ctggtgttga ccgtattatg acgatggact tacacgctga tcaaattcaa
     1441 ggattttttg aagttccagt agatcacttg tttgcttcta ctatt
//
LOCUS       DAOQVQ010000556         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_118608_length_1484_cov_3.802659, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000556 DAOQVQ010000000
VERSION     DAOQVQ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118608_length_1484_cov_3.802659"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKN22_11875"
     CDS             complement(<1..736)
                     /locus_tag="PKN22_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43448.1"
                     /translation="MILHIKNGVKTERIEAIAREINAFHIHYQGKVVLITESKVKEVP
                     ASIADDVDSFWVADSDIQLASKSYQREKRTIQLGNVTIGGATNNTVLIGGPCSVESEE
                     QIRSSSELLKSLNLSVLRGGCYKPRTSPYSFQGMGKEGLLLLAKMREEYGLTVITEAR
                     DATHIDEIIEYSDVIQVGAKAMYDQGILRACAKTTKPVLIKRGFGTTLQEFVQASEFV
                     LSGGNPNVILCERGIRTFETKTRFTLD"
     gene            complement(824..>1484)
                     /locus_tag="PKN22_11880"
     CDS             complement(824..>1484)
                     /locus_tag="PKN22_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS43449.1"
                     /translation="YQDFDFILIMATIPGQSGGVFDRVNFDKIRKFRNKFPAKSIHVD
                     GGVNGEVSFILRNMGVTSSVSGSYLFKGPSIGHQLMNLTKRKIESQYVVGDFMTPINE
                     CPVVNEAELTFSNIICAIENGNVGFTLIVGPNNQFKGLVSNADVRKALIKHLDNLNQI
                     QPQEMINASPLTILEEATVIELLKTVKSTPFIVSYFPVLNQQGEAVGIVNFANLIKAE
                     L"
BASE COUNT          446 a          310 c          243 g          485 t
ORIGIN      
        1 aatcaagcgt aaaacgtgtt tttgtttcaa aagtacgaat tccgcgctcg cataaaatca
       61 catttggatt tccacccgat aaaacaaatt ctgatgcttg aacaaattct tgcaatgttg
      121 ttccaaatcc acgtttaatc aacactggct tggttgtttt ggcacatgca cgtaaaattc
      181 cttgatcgta catggctttt gcacccactt gaattacatc cgaatattca ataatctcat
      241 cgatatgtgt tgcatctctt gcttcggtaa ttactgttaa accgtattct tcgcgcattt
      301 ttgcaagtaa caacaagcct tctttgccca ttccttgaaa cgaataagga cttgttcttg
      361 gcttgtagca acctcctctc aacaccgata aattcaacga ttttaacaat tctgacgaag
      421 aacgaatttg ctcttccgac tccaccgaac acggtccgcc aattaaaacg gtattattgg
      481 tagccccacc aatggtaaca ttacccaatt gaatggttct tttttcacgt tggtaacttt
      541 ttgaagccaa ttgaatatct gaatcagcca cccaaaacga atcaacatca tctgcaatgg
      601 atgctggaac ttcttttact ttggattctg taatcaagac tacttttcct tgataatgaa
      661 tgtgaaaagc atttatttca cgtgcaatcg cctcaattct ttctgttttt acaccgtttt
      721 taatgtgtaa tatcatacta attttttaaa aaattcaaac aaaaataaca aaagttatcg
      781 ttgaagtagg cggtttgcgt gtgaacttat aaacaatttg aatctataat tcggctttta
      841 tcaaattcgc aaaattaaca attccaactg cttcaccttg ttggtttaac accggaaaat
      901 aggaaactat aaacggtgta gattttacgg ttttcaacaa ctcaatgact gtagcctcct
      961 ccaaaattgt taacggtgaa gcatttatca tttcttgggg ttgaatttgg ttgagattgt
     1021 ccaaatgttt tatcaatgct ttgcgcacat cggcgttgga taccaatcct ttgaactggt
     1081 tgttgggacc aacaattaat gtaaacccta catttccatt ttcaatagca cagataatgt
     1141 tggaaaacgt taattcggct tcgttcacaa ccggacattc attgatgggt gtcatgaaat
     1201 cgcccactac atattgagat tctatcttgc gtttagttaa attcattaat tggtgtccaa
     1261 tgctgggccc tttgaataaa taggaaccac tcaccgacga ggttactccc atgttgcgca
     1321 aaataaacga aacttctccg ttgacaccac cgtccacgtg aatggatttt gcaggaaatt
     1381 tattgcgaaa ctttctgatt ttatcaaaat ttactctatc aaacacacca ccgctttgtc
     1441 cgggaatggt tgccatgatt aaaataaaat caaaatcttg atag
//
LOCUS       DAOQVQ010000557         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_119254_length_1479_cov_4.934691, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000557 DAOQVQ010000000
VERSION     DAOQVQ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119254_length_1479_cov_4.934691"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKN22_11885"
     CDS             complement(<1..273)
                     /locus_tag="PKN22_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS43450.1"
                     /translation="MSIGNNLKLLRKRKKLSQEEIANALGLTRSSYSGYENGVAQPNI
                     DNLIAFSEYHTIPIDELVKKDFELFSEADWQRIENGTYTDVKGIDRK"
     gene            407..>1479
                     /locus_tag="PKN22_11890"
     CDS             407..>1479
                     /locus_tag="PKN22_11890"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNS43451.1"
                     /translation="MKLLKLSLLLTTLLIVASCQKQFLSYFQKGQVQKLEKAVEIPFT
                     IENGLIIIPVEIEGDFFRFLLDTGAPNAISKELANKLNIEKSRSFKTEDSQGNRSELD
                     YAKIGKIKIGKAIFTNTTAAIADFNQIDAIACLNIDGLIGANLMRKAYWQIDNQVGVV
                     RIAPSRELLKKEPEEYSIPFITNISGTPLLNLRIGKTEIKRVTLDTGSVGFLSTGNEN
                     YKALRDGNQLLAERNAYGASSFGLFGSSKNDTLRQVLINDFSMGDLTLNQQVIELRNS
                     KASLLGMSFLRNYLVTIDWNSTTLYLSPQTNFTNLYAESFGISLFKEKEKLVVAMVTE
                     GSSAQRAGIKVGDIVLQVNDIDV"
BASE COUNT          498 a          238 c          253 g          490 t
ORIGIN      
        1 cttccgatct atacctttca catcggtata ggtgccattt tctatacgtt gccaatcggc
       61 ttctgaaaat aattcaaaat cttttttcac caattcatct attggaatgg tatgatattc
      121 tgaaaaagca attaaattat caatgtttgg ttgtgcaaca ccattttcgt atccactgta
      181 ggatgaacgc gttaaaccaa gtgcatttgc aatttcttct tgcgacaatt tttttctttt
      241 gcgcaacaat tttaaattat tcccgatgct catttccaaa atttattgta aatataaata
      301 aaaaactaaa taataatcat tttgttgaca atattttaat cattttggcg ttattttata
      361 aaatgaaaaa ttttgttgaa cattaaaaaa tgtttaaatt agtgcaatga aattgctgaa
      421 attaagtctt ctcctaacta cgttgttaat agttgcttca tgtcaaaagc aatttctaag
      481 ctattttcaa aaagggcaag ttcaaaaatt agaaaaagca gtagaaattc cgttcacaat
      541 agaaaatgga ctcatcatta ttccagttga aatagaaggc gatttttttc ggtttttatt
      601 agacacaggt gctcccaatg caatttcgaa agagttggcc aataaattga acatcgaaaa
      661 atcacgtagt ttcaaaacag aggattcaca aggcaatcgt tcagaattgg attatgccaa
      721 gattggaaaa attaaaatcg gaaaagcaat ttttacaaat acaactgctg caattgcaga
      781 ttttaatcaa attgatgcca tagcctgttt gaacattgat ggtttaattg gagctaattt
      841 aatgcgcaaa gcttattggc aaattgataa tcaagttggt gttgtgcgca ttgcaccatc
      901 gcgtgagctg cttaaaaaag aacccgaaga atacagcatt cctttcatta ccaacatttc
      961 tggcactcca ttgcttaatt tgagaatagg aaaaactgaa atcaaacgtg taacattgga
     1021 tacagggtcg gttggatttt tatcaactgg caatgagaat tacaaagcat taagagatgg
     1081 aaatcagttg cttgccgaac gaaatgctta tggcgctagt tcttttggat tattcggttc
     1141 atctaaaaac gatacgttaa ggcaagtgtt gataaacgat ttttctatgg gtgatttaac
     1201 attaaatcaa caagttatag aattaagaaa ctctaaagct tcccttttgg gtatgtcttt
     1261 tttacgcaat tatttggtga ccatcgattg gaattctacc accctttact taagtccaca
     1321 aaccaacttc accaatctat atgcagaatc ttttggtatt tcacttttta aagaaaaaga
     1381 aaaattagtg gttgccatgg ttaccgaagg tagttctgca caaagagcag gcattaaagt
     1441 tggcgatatt gttttgcaag tcaacgatat tgatgtgga
//
LOCUS       DAOQVQ010000558         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_119872_length_1475_cov_2.450000, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000558 DAOQVQ010000000
VERSION     DAOQVQ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119872_length_1475_cov_2.450000"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1053
                     /locus_tag="PKN22_11895"
     CDS             259..1053
                     /locus_tag="PKN22_11895"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNS43452.1"
                     /translation="MKQYHELLQHILDNGVKKEDRTGTGTISVFGHQSRYDLSQGFPV
                     VTTKKVHLRSIIHELLWFLQGDTNIKYLKDNGVSIWDEWADENGNLGPVYGHQWRSWP
                     GKNGETIDQISKLIEQIKKNPDSRRLIVSAWNVADVDNMALPPCHTLFQFYVAEGKLS
                     CQLYQRSADTFLGVPFNIASYALLTMMIAQVCDLQPGDFVHTLGDAHLYSNHIEQAKL
                     QLSRELRPLPTMKINPEVKDIFAFKFEDFELVNYDPHPHIKAEVAV"
     gene            1058..>1475
                     /locus_tag="PKN22_11900"
     CDS             1058..>1475
                     /locus_tag="PKN22_11900"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNS43453.1"
                     /translation="MKVALIVAVDKQGGIGKNNDLPWHMPDDMKFFKTTTTNHIVVMG
                     RKNYESIPERFRPLPNRENVILSRNSNFTAPNCLVFNSLEACFEHYKEETERIVYIIG
                     GGEIFKQALESNQVSEMFITHINYQFDCDVFFPNFDK"
BASE COUNT          483 a          250 c          279 g          463 t
ORIGIN      
        1 ttagaggtgt gtggtcccat gcaaactacg catttactca cagttggagg tgttttatca
       61 attttccacc caaacaatga aagtataaac aagccaattt ttcccatggt tcaaaattaa
      121 tttttttcta aatatggatt aaaaaaatgc atgaaattca attaataaac aaccgttaat
      181 aaatcgtgat aacttatttt caaatgcact tgtattcttt ctatttttgt atagcaaaca
      241 acaacaagct aatttaacat gaaacaatac cacgaattgc tccaacacat tttggataac
      301 ggagttaaaa aagaagacag aacaggtacg ggaactattt ctgtatttgg gcaccaatcg
      361 cgttatgatt tatcgcaagg ttttccagtt gttaccacta aaaaagtgca tttgcgctcc
      421 attattcatg aattgttgtg gtttttgcaa ggtgatacta atatcaagta tttgaaagat
      481 aatggtgtga gtatttggga cgaatgggcg gatgaaaatg gaaatcttgg tccagtttac
      541 ggtcatcagt ggagaagttg gccaggaaaa aatggcgaaa ctattgatca aatttcaaaa
      601 ttgatcgagc aaatcaaaaa aaatccagat tctcgtcgtt taattgtttc tgcgtggaac
      661 gtagctgatg tggataacat ggcgttgcct ccttgtcata ctttgtttca attttatgtg
      721 gcagaaggaa aattaagctg tcagttgtac cagcgtagtg ctgatacgtt tttaggagtg
      781 ccgtttaaca ttgcttcgta tgcattatta accatgatga ttgcacaagt ttgcgattta
      841 caacctggtg attttgtaca cactttgggt gatgcacact tgtattctaa ccatatcgaa
      901 caagcaaaat tgcagttgtc gcgtgagttg cgcccattgc caaccatgaa aattaatcca
      961 gaggtgaaag atatttttgc cttcaagttt gaagattttg aattggtaaa ttacgatcca
     1021 catccacaca tcaaagcaga agttgctgta taaaaatatg aaagtagctt taattgttgc
     1081 cgttgacaaa caaggtggaa taggtaaaaa caacgatttg ccttggcaca tgccagatga
     1141 catgaaattt ttcaaaacca ccacaaccaa tcatattgtg gtgatgggaa gaaaaaatta
     1201 cgaatccatt ccagaacgtt ttcgcccgct acccaatcgt gaaaacgtta ttttgtcaag
     1261 aaattctaat tttacggcac ctaattgttt ggtattcaat tcgcttgaag cttgttttga
     1321 acattacaaa gaagaaactg aacgaatagt gtacatcatc ggtggtggtg aaatttttaa
     1381 acaagcactt gaatccaatc aagtttcaga aatgtttatt acgcacatca attaccaatt
     1441 tgactgcgac gtgtttttcc ctaattttga taaaa
//
LOCUS       DAOQVQ010000559         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_122013_length_1459_cov_2.891738, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000559 DAOQVQ010000000
VERSION     DAOQVQ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122013_length_1459_cov_2.891738"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKN22_11905"
     CDS             complement(<1..385)
                     /locus_tag="PKN22_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43454.1"
                     /translation="MKKVYTLLMAVLSFNAFSQTTTLYSDDFEGASSFQFQYYPVSSA
                     NTWKIESCAGNGITNTGSKALYTSKGGINPGCGVNGTEQYANAPALPSSTERIIALMS
                     IDGRCTNSHSITFDYKLDLTGANNLV"
     gene            519..941
                     /locus_tag="PKN22_11910"
     CDS             519..941
                     /locus_tag="PKN22_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43455.1"
                     /translation="MLIDSSSITQYIPQRAPFVMVDTLVAASETNFESQFMVKNTNIF
                     LKDGVLSESALLENIAQTCAAGFGFVNSQKGEEAGKLGFIGSITRVEIKNVAKENDLL
                     TTKVTVLSTFDAIHLIEGRVFDSLQNELISCQMKIVVG"
     gene            complement(938..>1459)
                     /locus_tag="PKN22_11915"
     CDS             complement(938..>1459)
                     /locus_tag="PKN22_11915"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNS43456.1"
                     /translation="NIESEINQLKQFAPVWVSDVETLQQANEMILTVGEMVGKSVESQ
                     KIVAEIEANFSRLTHKNKWTCLYFMWKDPYMVAGNDTFITEMIRYCGGINLQQANRYP
                     VWEFPEDSEPDVVLLSSEPYPFEQKHVAFFQEKYPNSKIVLVEGEYFCWYGSRLRNVP
                     AYFEEINELIQHS"
BASE COUNT          454 a          268 c          259 g          478 t
ORIGIN      
        1 ccactaaatt attagctccg gtcaaatcca atttataatc aaacgtaatg gaatgtgaat
       61 ttgtacatct tccatctatg ctcattagtg caataatgcg ctcggttgaa cttggcaaag
      121 caggtgcatt ggcatattgc tctgtaccat taacaccaca accaggattg attcctcctt
      181 ttgaagtgta gagtgcttta gaacctgtat tggtaattcc atttcctgca caactctcta
      241 ttttccatgt gttagctgaa cttacaggat agtattgaaa ttgaaaggaa gatgcacctt
      301 caaaatcatc actgtacagt gtagtggttt gagagaaagc attaaagctc aaaactgcca
      361 ttaagagcgt gtaaactttt ttcatattca taagttaatt tatagttggt aaatatatac
      421 aaaactttca tcaaaaaagt ggcatttttt aaaaattgat taacaagtta ttaagaatgt
      481 gttgtaaaat gtatatttgt tgcacacaaa ttattcgtat gttaattgat agctcgtcca
      541 ttacccaata cattccacaa cgtgcaccat tcgtgatggt tgacacactt gtagctgcta
      601 gtgaaactaa ttttgaatcg caatttatgg taaaaaacac caatattttc ttaaaagatg
      661 gtgttttatc cgaatcggca ttgttagaaa atattgctca aacttgtgct gctggatttg
      721 gttttgtaaa cagtcagaaa ggtgaagaag ctggaaaact gggatttatt ggctctatta
      781 cgcgagtaga aattaaaaac gtagcaaaag aaaatgattt attaaccacc aaagttactg
      841 ttcttagtac gtttgatgcc attcacctca tcgaagggcg tgtttttgat tcgcttcaaa
      901 acgaattgat ttcgtgccaa atgaaaattg tggtgggcta agaatgttgg attaattcgt
      961 ttatttcttc aaaataggca ggtacattgc gcaagcggga accgtaccaa caaaaatatt
     1021 ctccttctac caacacaatt tttgaattgg ggtatttttc ttgaaaaaag gcaacgtgtt
     1081 tttgctcaaa aggatagggc tctgaactca acaacacgac gtcgggttca ctatcttctg
     1141 gaaattccca aaccggataa cgattggctt gttgcaaatt tattccacca caatagcgta
     1201 tcatttcggt aataaacgta tcgtttcctg ctaccatgta agggtctttc cacataaaat
     1261 acaaacaggt ccacttgttt ttatgggtca accgactaaa attcgcttca atttcagcta
     1321 caattttctg gctttcaacc gattttccca ccatttcacc cactgttaaa atcatttcat
     1381 tggcttgttg caaggtttca acatcgctta cccaaacagg tgcaaattgc tttaattgat
     1441 tgatttcgga ttcaatgtt
//
LOCUS       DAOQVQ010000560         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_122555_length_1455_cov_2.892143, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000560 DAOQVQ010000000
VERSION     DAOQVQ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122555_length_1455_cov_2.892143"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKN22_11920"
     CDS             <1..198
                     /locus_tag="PKN22_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43457.1"
                     /translation="GSGEATGGSDWKKYMLPQYQRRIFFPELWTDEELKNWGVDNLEG
                     YKTITNIDELFTNKTFQGWQK"
     gene            186..692
                     /gene="purE"
                     /locus_tag="PKN22_11925"
     CDS             186..692
                     /gene="purE"
                     /locus_tag="PKN22_11925"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNS43458.1"
                     /translation="MAKVGIIMGSKSDLPIMQEAADILEKFGIAYELNIVSAHRTPDL
                     MVDYARNAKKRGVNVIIAGAGGAAHLPGMTASLTALPVIGVPIKSSNSIDGWDSILSI
                     LQMPNGIPVATVALNGAKNAGILAAKILGASNDQILAKVEEYMIDLKEAVIKSQEDLN
                     ESKSGIDD"
     gene            851..1291
                     /locus_tag="PKN22_11930"
     CDS             851..1291
                     /locus_tag="PKN22_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNS43459.1"
                     /translation="MSTRDIQEVVKNIFSAYLEQNGHRKTPERFAILEEIYSYPGHFD
                     VETLYIKMKSQNYRVSRATLYNTIELLLACNLVRKHQFGKNISQFEKSHGNKQHDHLV
                     LTDTGEVIEFCDPRIQIIKSTIEEVFGVEVQHHSLYFYGVKKEQ"
BASE COUNT          511 a          219 c          294 g          431 t
ORIGIN      
        1 ggttcgggtg aggcaaccgg tggaagtgat tggaaaaaat acatgttgcc acaataccaa
       61 cgcagaattt tcttcccaga attgtggaca gatgaagaat tgaaaaattg gggtgtagat
      121 aatttggaag gttataaaac aattactaat attgacgaat tatttacgaa taaaacattt
      181 caaggttggc aaaagtagga atcatcatgg gaagcaaatc ggatttaccg attatgcaag
      241 aagcagctga tattttagaa aaattcggta ttgcttatga attaaacata gtttcagcac
      301 atagaacacc tgatttaatg gtggattatg cacgaaacgc aaagaaaaga ggggtaaacg
      361 tcattattgc tggtgctgga ggtgcagcgc atttgccagg aatgacagct tcattaacag
      421 cattgcctgt tatcggtgtg cctattaaat catctaattc tattgatggt tgggacagta
      481 ttttatccat tcttcaaatg cccaacggta ttcctgttgc aacagttgct ttaaatggag
      541 caaaaaacgc aggtatcttg gccgctaaaa ttttaggagc aagcaacgat caaattttgg
      601 caaaagtgga agaatacatg attgacctta aagaggcagt gattaaaagt caagaagact
      661 taaacgaaag taaaagtggt attgacgatt aattgttaac cataaattga ttgaaaagag
      721 gtttctattg gaagcctctt tttgtttttg tgactatctg tcccatttat caacaattgt
      781 ttaaaaactt gtggtaaaat ttagggcttt tacaaataaa attacttaga tttgcctttt
      841 tataaatgtt atgagtactc gcgatattca agaagtagta aaaaacatat tttcagctta
      901 tttggaacaa aatgggcacc gaaaaacacc cgaaagattt gcaattttag aggaaatcta
      961 tagttacccc ggacattttg atgtggaaac attgtacatt aaaatgaaaa gtcaaaatta
     1021 ccgcgtttct cgtgcaactt tgtacaatac catcgaattg ttattggcgt gtaacttggt
     1081 gcgtaaacac caattcggga aaaatatctc tcaatttgaa aaatcgcacg gaaataaaca
     1141 acacgaccat ttggtgttga ccgatacagg agaagtaatt gagttttgcg acccgcgcat
     1201 tcagattata aaatcaacta ttgaagaagt gtttggcgtt gaggtacaac accattcctt
     1261 gtatttttac ggtgttaaaa aagaacaata acccatcatg cagtatcaga tttcaattgt
     1321 tgagcatgtt ttaatcgttt ccttggaagg aaaaatgatt gaagaaaatg tctataccga
     1381 cttattggct caaattgaaa accgattgaa cgaaaccaat ggaaagttga taataaacgt
     1441 tgaaaaatta gatta
//
LOCUS       DAOQVQ010000561         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_122941_length_1452_cov_4.609878, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000561 DAOQVQ010000000
VERSION     DAOQVQ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122941_length_1452_cov_4.609878"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKN22_11935"
     CDS             <1..1138
                     /locus_tag="PKN22_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNS43460.1"
                     /translation="QRLKEAAEKAKIELSSTTSTEINLPYIMPVNGIPKHLVRTLQRA
                     KFEQLISDIVERTIAPCRSALQNAGLSTSDINEVILVGGSTRIPVIQDAVERFFGKAP
                     SKGVNPDEVVALGAAIQGGVLTGEVKDVLLLDVIPLSLGIETMGGVFTKLIEANTTIP
                     TKKSETFSTASDNQPSVDIHVLQGERSMAADNKTLGRFQLSDIPPAPRGVPQIEVTFD
                     IDANGIMNISAKDKGTGKQQSIKIESSSGLSDEEIAKMKADAEAHAEADKARAKEAET
                     INKADSLIFQTERQLKEYGDKIPADKKQPIEDALAELKKEFEAKNVANLEPAMEKLNN
                     VFQAASQDMYNAANAQGGADANQGSTNPNDEVTDVDFEEVDDKK"
     gene            1238..>1452
                     /locus_tag="PKN22_11940"
     CDS             1238..>1452
                     /locus_tag="PKN22_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43461.1"
                     /translation="MEKFKVTVYPNTSSPKLFKNKFLEFLTKTHPIVIDMMYATISFF
                     MIRHYITTQASYSVKLIVGLFLMGFLS"
BASE COUNT          519 a          262 c          267 g          404 t
ORIGIN      
        1 tcaacgtttg aaagaagctg ctgaaaaagc aaaaattgaa ttgtcttcaa caacttcaac
       61 agaaatcaac ttgccataca tcatgcctgt aaatggtatt ccaaaacact tggtaagaac
      121 tttacaacgt gctaaatttg aacaattaat ttctgatatc gttgaaagaa caatcgctcc
      181 ttgtcgttct gctcttcaaa acgcaggatt gtcaacaagc gacatcaacg aagtaatctt
      241 ggtaggtggt tctacacgta tcccagtaat tcaagatgca gttgaacgtt tcttcggaaa
      301 agctccttca aaaggtgtaa atccagacga agttgttgct cttggagctg ctattcaagg
      361 tggtgtattg acaggagaag taaaagatgt attgttatta gacgttattc cattgtcact
      421 tggaattgaa acaatgggtg gtgttttcac taaattaatt gaggcaaaca caaccattcc
      481 aactaaaaaa tcagaaactt tctctacagc atctgataac caaccatctg ttgacatcca
      541 tgtattgcaa ggtgaacgtt caatggctgc tgataacaaa acattgggtc gtttccaatt
      601 gtcagatatt ccaccagcac caagaggagt tcctcaaatt gaagtaactt ttgacattga
      661 tgctaacgga attatgaata tttctgcaaa agataaagga acgggtaaac aacaatccat
      721 caaaattgaa tcatcatccg gtttatctga tgaagaaatt gcaaaaatga aagcagatgc
      781 agaagcacac gcagaagctg ataaagcaag agctaaagaa gctgaaacaa tcaacaaagc
      841 agattcattg attttccaaa cagaacgtca attgaaagaa tacggtgata aaatcccagc
      901 ggataaaaaa caaccgattg aagatgcgtt ggctgaattg aaaaaagagt ttgaagcgaa
      961 aaacgtggct aacttggaac ctgcaatgga aaaattaaac aacgttttcc aagcagcttc
     1021 acaagacatg tacaacgctg caaatgctca aggtggcgct gatgcaaatc aaggtagcac
     1081 caatccaaac gacgaagtta ctgatgttga cttcgaagaa gtggacgaca aaaaataaga
     1141 ataacaattc attgggagag gctgcaaatt ttgtagcctc ttttttttgt ttgaaacttt
     1201 ttttgaataa ttttgagcaa ctacaattac acaaaatatg gaaaagttta aagtaacagt
     1261 ttatcctaat acttcttcac caaaactttt taaaaacaag tttttagaat tccttaccaa
     1321 aacgcatccc atcgtaattg acatgatgta tgcaactatt tcatttttta tgatacggca
     1381 ttacatcact acacaagcgt cctattcagt taaactaatt gtaggattgt ttttgatggg
     1441 gtttctatcg tg
//
LOCUS       DAOQVQ010000562         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_123019_length_1452_cov_2.340730, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000562 DAOQVQ010000000
VERSION     DAOQVQ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123019_length_1452_cov_2.340730"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..865)
                     /locus_tag="PKN22_11945"
     CDS             complement(104..865)
                     /locus_tag="PKN22_11945"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNS43462.1"
                     /translation="MIEKINIKEELDVRVYVPIQRHEMLIELFKELPVNESFVFINDH
                     DPLPLFYEFRSIYGDVVGWEYLNRGGREWKVKVTRTEASIGREFTDISTLIDLRKVEP
                     ADWKQVVFHRYGMMEEGTTMEIIAKEDPKEIHGIFINKFEGKHAWNYKKKDEGEYVVH
                     ITKKDNSGLGDTGFSVVNEFDIRPFPPTERHEMFYKAFADIQPGEAFEFINDHDPLPL
                     YYQMEAESKDPFRWEYLEKGPDAWKVRVIKVKKEA"
     gene            complement(876..1307)
                     /locus_tag="PKN22_11950"
     CDS             complement(876..1307)
                     /locus_tag="PKN22_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNS43463.1"
                     /translation="MLSSTCKTAIKAVIYLSSKNCCEEKVGIKEIAEKINASEHTVGK
                     TLQTLVKHKIINSLKGPSGGFYILPDQRLQPISVIVYTIDGSDVFKQCGLGLSQCSES
                     HPCPIHNEYKEARVLIEKLFNEKRIADLCDPVANGLAFLIG"
BASE COUNT          409 a          300 c          247 g          496 t
ORIGIN      
        1 gttgtttgca tgaactaaat gtaggaaaaa aaagtttatc agacatgaaa aaggctaccc
       61 acacaacatg agcagcctta aaatggtaga atttatatta aaattatgct tcttttttta
      121 ctttgataac gcgcactttc caagcatctg gtcctttttc taaatattcc caacggaaag
      181 gatctttgct ttcggcctcc atttgataat atagtggaag tgggtcgtgg tcgttgatga
      241 attcaaacgc ttctcctggt tgaatatctg caaatgcttt gtagaacatt tcgtgacgtt
      301 ctgttggtgg aaacggacga atgtcaaatt catttacaac agaaaatcct gtatcgccca
      361 agccactgtt gtcttttttg gtaatgtgca caacgtattc gccttcatct ttctttttgt
      421 aattccaagc gtgttttcct tcaaatttat tgatgaaaat tccatgaatt tcttttggat
      481 cttctttggc aataatttcc attgttgtgc cttcttccat cattccgtaa cggtgaaaaa
      541 caacttgttt ccaatcagct ggttccactt ttcgcaagtc gattaaggtt gaaatatccg
      601 taaattcacg cccgatagat gcttcagtac gagtcacttt tactttccat tctcttccgc
      661 cacggttgag gtattcccaa cctacgacat cgccataaat ggaacgaaac tcgtaaaata
      721 atggaagtgg atcatggtcg ttgatgaaca caaaactttc gtttactggc aattctttaa
      781 acaattcaat aagcatttca tgacgttgga tgggaacata tacacgcacg tcaagctctt
      841 ctttgatatt aattttttca atcatactat ttgttttatc ctattaaaaa tgcaagaccg
      901 ttggcaacag ggtcgcacaa gtctgcaatg cgtttttcgt taaataattt ttctatcaat
      961 acccttgctt ctttgtattc attgtgaata gggcaaggat gcgattccga acattgactc
     1021 aaacccaatc cacattgctt aaacacatca cttccatcaa tggtataaac aattaccgaa
     1081 atgggctgca aacgttggtc gggaagaata taaaaaccac cacttggtcc tttgagacta
     1141 ttgataattt tatgtttaac aagtgtttgt agtgttttgc ccaccgtatg ttcgctagca
     1201 ttgatttttt cagcaatttc tttaatgcct actttttctt cgcagcaatt tttactcgat
     1261 aggtaaataa cggcttttat ggctgtttta caggttgaac tcagcatatt ctattgacct
     1321 aaaaattctc taccaacatc cgaaatctta gagtaatccc acgcaggttc aaaggttaat
     1381 tccacttcaa cttgccaatc tggaaaggct gtttttagtg ccccagttac atctcctgta
     1441 atctgatctc cc
//
LOCUS       DAOQVQ010000563         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_125097_length_1437_cov_3.407381, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000563 DAOQVQ010000000
VERSION     DAOQVQ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125097_length_1437_cov_3.407381"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKN22_11955"
     CDS             <1..305
                     /locus_tag="PKN22_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HNS43464.1"
                     /translation="SGYKAAEMTVKQLNGEVVDWENDYSNYMKKGINVFRTYVDGWYD
                     GRLGTIFFAKERNEEIMKQICSVLAGYVWDENNPFVKKHDKVVTSLARVIEINEGK"
     gene            complement(318..836)
                     /locus_tag="PKN22_11960"
     CDS             complement(318..836)
                     /locus_tag="PKN22_11960"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNS43465.1"
                     /translation="MNVILVGYMGSGKTTLGKKLAARLGLTFMDTDKIIEEQEAQSVD
                     EIFATKGEAYFRKLEQALVEELKQLDHVLIATGGGMPCYNNLMEELKTIGFTIYLKRP
                     AKELAHRVKNSKKIRPLLRDKNEEELLAYMEAMLQKRAPYYEQAHLIVDREVQAIASL
                     ELQIKALTRNWK"
     gene            991..>1437
                     /locus_tag="PKN22_11965"
     CDS             991..>1437
                     /locus_tag="PKN22_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNS43466.1"
                     /translation="MREEAYIVTGFRTAVGKAFKGGFRFYRPDDLAAEVIKHLMNSVP
                     QLDKNRVDDVIVGNATPEAEQGLNVGRLISLMGLNTDKVPGMTVNRYCSSGLETIALA
                     KAKIEAGFADCIIAGGVESMSIMEMGGWRIVPNPKIGKEHPTWYWGM"
BASE COUNT          442 a          251 c          294 g          450 t
ORIGIN      
        1 tttctggtta caaagctgcc gaaatgactg taaaacaatt gaatggtgaa gtagttgatt
       61 gggaaaacga ttacagcaac tacatgaaaa aaggaattaa cgtatttaga acctacgtgg
      121 atggctggta tgacggacgt ttgggcacca ttttctttgc aaaagaacgt aacgaagaaa
      181 taatgaagca aatttgctcg gtattggcag gctatgtttg ggacgaaaac aatccttttg
      241 tgaaaaaaca cgataaagtg gtcacatcac ttgcacgtgt tatagaaatt aatgagggga
      301 agtaatttcc ctctttttta tttccaattt ctggtgagtg ccttgatttg tagttctaaa
      361 cttgcaatgg cctgcacttc tctatccaca atcaaatgtg cttgctcgta gtagggtgct
      421 cgtttttgca acattgcttc catgtatgct agtaattctt cctcgttttt atcacgcaaa
      481 agaggacgga ttttcttaga gttttttaca cggtgtgcca attcctttgc aggacgtttg
      541 aggtaaatgg taaaaccaat tgttttcaat tcttccatta agttattgta acacggcatt
      601 ccaccacctg tagcaattaa tacatggtcg agttgtttta attcttccac cagcgcttgt
      661 tccaatttcc gaaagtaggc ttctcctttg gttgcaaaaa tttcatccac cgattgcgct
      721 tcttgttctt caattatttt atcggtgtcc ataaaggtta atcccaacct tgcagccaat
      781 ttcttaccca aagttgtttt accagaaccc atatatccaa ccaaaattac attcattgcc
      841 tttattacac tttcctattg gtcaaaagta cgaaataaaa aaaacattaa aaaagtatgc
      901 atacataata tttttacaga aaaatgttta cctttactcg catagttgat tgggttcagc
      961 taccatgaat tagaaaaaac attttttaaa atgagagaag aagcatatat agttacagga
     1021 tttagaacag ctgtaggaaa agcatttaaa ggtggttttc gtttttacag acctgatgat
     1081 ttagcagcag aggttattaa acatttgatg aattcggttc ctcaattaga caaaaaccga
     1141 gtggacgatg ttattgttgg aaacgcaacg ccagaggcag aacaaggatt gaatgtgggg
     1201 cgtttaattt cattaatggg attgaatacc gacaaagttc cgggaatgac ggttaatcgt
     1261 tattgttcat ccggattgga aaccatcgcc ttagcaaaag caaaaattga agcgggattt
     1321 gccgattgca tcattgccgg aggtgttgaa tcgatgtcga ttatggaaat gggtggatgg
     1381 cgcattgttc ccaatccaaa aataggaaaa gagcatccaa cgtggtattg gggtatg
//
LOCUS       DAOQVQ010000564         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_125227_length_1436_cov_4.067343, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000564 DAOQVQ010000000
VERSION     DAOQVQ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125227_length_1436_cov_4.067343"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PKN22_11970"
     CDS             complement(<1..990)
                     /locus_tag="PKN22_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HNS43467.1"
                     /translation="MKTNEEIKALIAAVQAKSSGNFAIDELALEQEYQQIAQQPTIGI
                     RILSIIGGFMASILLFIFILISGLERSDIAQVVIGVILITATLMVSKNNKNTLLDTVL
                     VSAYLLGFLLVILGWINFDFWEQAIHLIIIALAIVSLIVVQSYILSFLATLLIFGSIL
                     SYLSVHEHFELIHAYNTLLAALVTFIAFNEAKIIAFNKATSHLYSPIKSALIVVFVAG
                     LFVVGKKDVFPLQTSTIWISSVASVPLIFYTIYRILPTLGINSHNTKTLFLLLSALAL
                     LPLFFSPAVAGAILILLTCFYNNSKGGFVLGIIALINFIVQFYYDLSINLLFKS"
     gene            complement(977..>1436)
                     /locus_tag="PKN22_11975"
     CDS             complement(977..>1436)
                     /locus_tag="PKN22_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNS43468.1"
                     /translation="LYAEQVAENWSEILVYTLLVVLNALFYIIATRYFKEQTPTWLLH
                     TVGLAIAFCATIGVIAGIFSGFKIEFLFLLLIATSLFGGGIWYGLTSKSSYLLAIIPF
                     CIIAICSALMFKISNHELMFFAVSIFVIGSVTFTIKNLFNLQKKWAHENK"
BASE COUNT          571 a          235 c          239 g          391 t
ORIGIN      
        1 tgatttaaag agcaaattga tactcaaatc gtaataaaat tgaactataa aattgattaa
       61 ggcaattatc cccaacacaa aacctccttt actgttgttg taaaaacaag ttaataaaat
      121 taaaattgca ccagctacag caggagaaaa aaacaatgga agcaaagcca atgcactgag
      181 caaaagaaat agtgtttttg tgttgtgaga attaattccc aatgttggta aaatacgata
      241 aatggtataa aaaataagtg gaacacttgc cacagacgat atccaaatgg tggacgtttg
      301 gagtggaaaa acatcctttt tgcctacaac aaacaatcct gctacaaata ccacaattaa
      361 agccgatttt ataggcgaat aaagatgaga agtagctttg ttgaatgcaa taattttagc
      421 ttcgttgaat gcgataaatg taactaatgc agccaataaa gtgttgtatg catgaataag
      481 ttcaaagtgc tcgtgcaccg acaaataact taaaatactt ccaaaaatta ataatgtagc
      541 aagaaacgat aaaatatagc tttgaacaac aataagcgaa acgattgcaa gtgcaatgat
      601 tattaaatga atggcttgct cccaaaaatc aaaatttatc catcccaaaa taaccaataa
      661 aaatcctagc aaatatgcag aaaccaaaac agtgtctaac aacgtgtttt tattgttttt
      721 ggaaaccatt aaagtagctg taattaatat tactccaata acaacttgag caatatccga
      781 gcgttctaat ccactgatga gaataaaaat aaacagtaaa atacttgcca taaatccacc
      841 aattatggaa agaatgcgaa ttccaatagt tggttgttgg gcaatttgtt ggtattcttg
      901 ttccaaagca agttcatcaa tggcaaaatt accagacgat tttgcttgaa cagcagcaat
      961 tagggcttta atttcttcat ttgttttcat gtgcccattt tttttgaagg ttgaataaat
     1021 ttttaattgt aaaagtaaca ctgccaatta caaaaatact tacggcaaaa aacataagtt
     1081 catggttgga aattttaaac attaaagccg agcaaatggc aatgatgcaa aacggaatga
     1141 tggcaagcaa atagctactt ttggaggtta aaccatacca aattccacca ccaaataggg
     1201 aagtggcaat aagcaataag aataaaaatt caattttaaa accagaaaaa attccagcaa
     1261 ttacaccgat agttgcacaa aaagcaattg ccaaaccaac ggtgtgaagt aaccatgttg
     1321 gcgtttgttc tttaaaatat cgggtggcaa taatgtaaaa aagagcatta agaacaacca
     1381 gtaatgtgta aacaagaatt tctgaccaat tttcggcaac ttgctcagcg taaagc
//
LOCUS       DAOQVQ010000565         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_128007_length_1418_cov_1.885547, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000565 DAOQVQ010000000
VERSION     DAOQVQ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128007_length_1418_cov_1.885547"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKN22_11980"
     CDS             complement(<1..548)
                     /locus_tag="PKN22_11980"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNS43469.1"
                     /translation="MATIGFFDGVHLGHRFLLNQLNMYAAKFGLESLVVSFVDSPQRI
                     LSPNSDIKLLTTSSEKLAIFSELGLQNCLMLNFDENMAAITSFDFLKLLHDTYRVQKL
                     VVGYDHHFGSDRLRTFDDYVQLGKKIGVEVIACQPFISENLTVSSSKIRTFLLEGDIM
                     KANELLGSPYTMQGVVVSGNQIG"
     gene            complement(724..>1418)
                     /locus_tag="PKN22_11985"
     CDS             complement(724..>1418)
                     /locus_tag="PKN22_11985"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS43470.1"
                     /translation="LMKEIQTVLHLPTLPQTIECFDNSNISGEAAVAACVVFKKARPS
                     KKDYRKYTIKTVDGPDDYASMQEVVRRRYQRMINEGTTLPDLIITDGGKGQMEVVRQV
                     VEDELQLKIPIAGLAKDNRHRTSELLYGFPPAAIGLKPTDFLFKFLASIQDEVHRFAI
                     SFHRDKRSKNQIASELDTIRGIGEKTKIELLQHFKSIKRIQNAEKNDLEKIVGTHKAS
                     IIYNYFHPEITA"
BASE COUNT          474 a          277 c          241 g          426 t
ORIGIN      
        1 cctatttgat tacccgacac cacaacccct tgcatcgtat aaggtgaacc aagtaattcg
       61 ttggctttca taatatctcc ttccagcaag aacgtacgaa ttttagaaga actaactgtt
      121 agattttcag aaataaaagg ctgacaagca ataacctcaa cacctatttt ttttcctaac
      181 tgcacataat catcaaatgt tctcaaccgg tcactcccaa aatgatgatc ataaccaacc
      241 actaattttt gcacccgata ggtatcatgc aataatttca agaaatcaaa tgaagtgatt
      301 gccgccatat tttcatcaaa attgagcatc aaacaatttt gcaaacccaa ttcagaaaaa
      361 atcgctaatt tttcggacga ggtagtcaat agtttaatat ctgaattggg agataaaata
      421 cgttgaggag aatcgacaaa cgaaaccacc aaagattcca atccaaattt tgcggcgtac
      481 atattcaact ggttcaacaa aaaacgatgc cctaaatgaa cgccatcgaa aaaaccgata
      541 gtcgccactg tattttgatt tggcctgttt atgttggaaa gaatgttcac ctttaaaaaa
      601 tattgagtga caaagttaaa caaaaaagaa gaaaaagaga aactttcaaa aaatggagaa
      661 aatagtgccc gaataattaa aaattaatgc cccaatagcg gacataaaaa acttacgcga
      721 ttctcaagcg gttatttcgg gatgaaaata attgtaaatt attgatgctt tgtgagttcc
      781 tacaattttt tctaaatcat ttttttcggc attctggata cgttttatac ttttgaagtg
      841 ttgaagtagc tcaattttgg ttttttcgcc aattccgcga atggtatcta gttcagaagc
      901 aatttggttt ttacttcgct tgtcgcgatg aaacgaaatg gcaaatcgat gaacttcatc
      961 ctgaattgat gccaaaaatt taaataaaaa atcagtcggt tttaaaccaa tggctgccgg
     1021 tggaaagcca tacaacaatt cactagtacg atggcgattg tctttggcta aacctgcaat
     1081 aggaattttt agttgaagct catcttctac cacttgacgc acaacttcca tctgaccttt
     1141 acctccatcg gttataatta agtcgggtag agtagtgcct tcattaatca ttcgttgata
     1201 ccgtcgacga acaacttctt gcatcgatgc ataatcatcg ggaccatcga cagtttttat
     1261 cgtgtattta cgataatctt tttttgatgg acgcgctttt ttgaatacca cacaagccgc
     1321 tacagcagct tcaccagaaa tattagagtt atcaaaacat tcgatggttt ggggtaaagt
     1381 gggcaaatgc aataccgttt gaatttcctt cattaagc
//
LOCUS       DAOQVQ010000566         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_128695_length_1413_cov_3.578792, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000566 DAOQVQ010000000
VERSION     DAOQVQ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128695_length_1413_cov_3.578792"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1413
                     /locus_tag="PKN22_11990"
     CDS             128..>1413
                     /locus_tag="PKN22_11990"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNS43471.1"
                     /translation="MKVLKFGGTSVGSAQRIKNLLSIIGSDESKIIVLSAVAGTTNAL
                     VEIADCLYQDKREEAHRKIQLLKDSYTQLIHDLFEIKTLDKAILLIDYHFDTIISLSN
                     DLFTPVEEKIILAQGELLSTALFHYYLEELNVRSVLLPALDFMKTDENNEPDIAFIRE
                     NIQSLLNNPTNEPILYITQGFICRNAFGEVDNLQRGGSDYTASLIGAAIASEEVQIWT
                     DIDGMHNNDPRVVANTRPIAFLSFEEAAELAYFGAKILHPQSIFPAQKFNVPVRILNT
                     MSPQSQGTLISQTGDANAVGVRSIAAKDGITAIRIQSSRMLLAYGFLRRVFEVFERYK
                     TPIDMITTSEVAVSLTIDNTRFLNEIIGELNDFGTVNVDENQTIICVVGTFEMDATGY
                     AAQIFEGIKHLPIRMISYGGSNHNVSLLVDSKNKAEA"
BASE COUNT          447 a          222 c          266 g          478 t
ORIGIN      
        1 caaaagcaca ttatgaaaat aatgtgcttt tgtattttac gttgtttaaa taacaaaaag
       61 ttaaaataat aacaatatgt tttattttta aattttaaat ttatttttgt gctcctaaat
      121 ttttaaaatg aaagtattaa aatttggtgg cacatcggtt ggatctgctc aacgtataaa
      181 aaatttactc tcaataattg gttcggacga atcaaaaatt attgtattgt cagcagttgc
      241 tggtaccaca aacgccttgg ttgaaattgc tgattgtttg tatcaagaca agcgagaaga
      301 agcacatcga aaaattcagc tattaaaaga tagttatacc caactaatcc atgatttatt
      361 tgaaattaaa actttagata aagctatttt gttaattgat tatcattttg atacgataat
      421 ttctttgtcc aacgatttgt ttactccagt agaagaaaaa ataatattag cacaaggcga
      481 attgttgtca acagctttgt ttcattatta cttggaagag ttaaacgtgc gttctgtttt
      541 gttacctgca ttggatttta tgaaaacaga tgaaaacaat gaacccgata ttgcttttat
      601 tcgtgaaaat atacaatcgt tgttgaacaa tccaacgaat gagcctattc tttacattac
      661 gcaaggattt atttgccgca atgcatttgg agaagtggat aatttgcaaa gaggaggttc
      721 ggattatacc gcatcgttga ttggtgctgc tattgcttcc gaagaagttc aaatttggac
      781 ggatattgat ggcatgcaca acaatgatcc acgtgtggtg gcaaataccc gcccaattgc
      841 ctttttgagt tttgaagaag cggccgagtt ggcttatttt ggtgctaaaa ttttgcatcc
      901 acaaagtatt tttccagctc aaaaattcaa tgtgcccgtt cgtatattaa acacaatgtc
      961 accacaatcg cagggaactt taatcagtca aactggtgat gcaaatgctg ttggagtgcg
     1021 ttctattgct gcaaaagacg gcattactgc cattcgcata caatcttcac gcatgttact
     1081 ggcatacggt tttttgcgcc gtgtttttga agtgtttgaa cgttacaaaa caccaattga
     1141 tatgataacc acttcagaag ttgctgtttc attaactatt gacaatacgc gctttttgaa
     1201 tgaaattatt ggtgaattga acgattttgg aaccgtaaat gtggatgaaa atcaaacgat
     1261 tatttgtgtt gttggcactt ttgaaatgga tgcaacgggt tatgctgctc aaatttttga
     1321 aggaataaaa catttgccta tccgtatgat ttcttatggt ggttccaatc acaatgtgtc
     1381 acttttagtg gattctaaaa acaaagcaga ggc
//
LOCUS       DAOQVQ010000567         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_130235_length_1403_cov_4.192878, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000567 DAOQVQ010000000
VERSION     DAOQVQ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130235_length_1403_cov_4.192878"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..501
                     /locus_tag="PKN22_11995"
     CDS             166..501
                     /locus_tag="PKN22_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNS43472.1"
                     /translation="MNFETIQNRNTAIIKTLVEKLDASNAGTLKSELIVLNKNGVNSI
                     VMDFSSTKYCDSSGLSAVLTANRLCKETSGKFILCGLNSTVGKLIEIAQLDKVLTITN
                     NLEEAKQLL"
     gene            502..>1403
                     /locus_tag="PKN22_12000"
     CDS             502..>1403
                     /locus_tag="PKN22_12000"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNS43473.1"
                     /translation="MSFEVTILGSGGALPTLNRNSTAQFVTCCNRHILIDCGEGTQLQ
                     MRKFGVHFQRLDIILISHLHGDHFFGLVGLLSTMRLLGRDGKLTIICPKGLPAIIQQQ
                     LEIGFQKLDFDIHYIELTGKEQQMVYEDKSIEIHCFPLKHKVPTSGFLIAEKPKERKM
                     KKDQLDHPLLKIEYIHRLKKGEDVTTEDGTVLLSKDFTDEPPVPLSYAYCSDTAYFEK
                     VVENIKNATTLYHEATFLEMHKDLAKATQHSTAKQAATIAKMANVGTLYMGHLSARYG
                     STNEFVAEAQEIFENAHYVEDGMK"
BASE COUNT          484 a          251 c          262 g          406 t
ORIGIN      
        1 aagctatttt ttaaaacata aaaaatggcc ggaatttctt ccgaccattc gtgagcgtta
       61 atgcgccttt ttgaagttat taacaattta gtgtcgtata aaaaataaac gttcattagt
      121 tgttgataag catttttcat tatttttgaa aaaacaagct aaataatgaa tttcgaaact
      181 attcaaaacc gcaatacagc aattatcaaa acgttggttg aaaaactcga cgcatccaat
      241 gcaggaacac taaaatctga attgattgta ttgaataaaa atggtgtcaa ctctattgtg
      301 atggattttt cttcaacaaa atattgcgat tcatcgggac ttagcgctgt tttaacggcc
      361 aatcgtttgt gcaaagaaac aagtggaaaa tttattcttt gtggcttgaa ttcaaccgtt
      421 ggtaaattga ttgaaattgc acagttagac aaagttttga ccattaccaa caacctcgaa
      481 gaagctaagc aattgctata agtgagtttt gaagtaacca tcttaggaag tggtggggcg
      541 ttgcctacat taaatcgaaa ttctaccgcc caattcgtta cttgctgtaa tcgccatatt
      601 ttaattgatt gtggagaagg aacacaattg caaatgcgca aatttggcgt tcatttccaa
      661 cgactcgata tcattttaat ttcccacctt cacggtgatc atttttttgg attggttggc
      721 ttgttaagca ccatgcgcct tttaggaaga gatggaaaat taaccattat ttgccccaaa
      781 ggtttgcccg ccatcattca acaacaatta gaaattggtt ttcaaaaatt agacttcgac
      841 attcattaca tcgagctaac aggcaaagag caacaaatgg tgtatgaaga taaatctata
      901 gaaattcatt gttttccgct caaacacaaa gtgccaactt cgggattttt aattgcagaa
      961 aagcccaaag aacgaaaaat gaaaaaagac caattagatc atccgctgtt gaaaatagaa
     1021 tacattcacc gtctcaaaaa aggagaggat gtgacgacag aagatggaac ggttttgttg
     1081 tctaaagatt ttacagatga accaccagtg cccctttcgt atgcttattg ctcggataca
     1141 gcctattttg aaaaagtggt agaaaacata aaaaacgcaa caacactcta tcacgaagct
     1201 acgtttttgg aaatgcacaa agatttggca aaagcaaccc aacattctac agcaaagcaa
     1261 gcggcaacca ttgcaaaaat ggccaatgtt gggactttat acatgggaca ccttagtgcg
     1321 cgctacggtt caaccaatga atttgtggca gaagcccaag aaatttttga aaatgcacat
     1381 tatgttgaag atgggatgaa att
//
LOCUS       DAOQVQ010000568         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_130578_length_1401_cov_4.115156, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000568 DAOQVQ010000000
VERSION     DAOQVQ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130578_length_1401_cov_4.115156"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKN22_12005"
     CDS             complement(<1..383)
                     /locus_tag="PKN22_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNS43474.1"
                     /translation="MKIALIGYGKMGKAIEEIAIERGHSIVAKINQSTPIESLKSVEA
                     DVAIEFTRPDAAVANIQFCLNHSLPVVVGTTAWYDAFEHVKNEVAQHNGALLHASNFS
                     VGVNIFFEINRMLAQLMQPYAEYTA"
     gene            complement(399..986)
                     /locus_tag="PKN22_12010"
     CDS             complement(399..986)
                     /locus_tag="PKN22_12010"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNS43475.1"
                     /translation="MKYVFLFLCWTVLQPVFSQEVAPINTKKEVERDSTKQHSVKLAA
                     ILSAALPGAGQVYNHIAMPKGKKKAYWKVPLIYAGLGVTGYFAVKNHLEQKVLKQEYI
                     NRTEYGLTPIEKYSNYDDAGILQLYNQKITRRDLLFLGMGLVYLLQIADAAVEAHFVN
                     FDVSKDLSMKIRPAVIPMDYSATPTVGISLQLSLK"
     gene            complement(987..>1401)
                     /locus_tag="PKN22_12015"
     CDS             complement(987..>1401)
                     /locus_tag="PKN22_12015"
                     /inference="COORDINATES: protein motif:HMM:NF036800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HNS43476.1"
                     /translation="TNFLRLLKLPAEIQAGVRDNVISMGHARSLVSAGDEAKQLEIYA
                     KVVAHSLSVRDTEALVKGELQLAATDKDTDSDASVKKSKSNTISESQYVFKEHLSDRL
                     ATKVDIKKAANGSGTIVLKFSSENDLNRIIELLNN"
BASE COUNT          405 a          297 c          241 g          458 t
ORIGIN      
        1 cttgccgtat attctgcata gggttgcatc aactgcgcca acatccggtt gatttcaaaa
       61 aagatgttca ctcccaccga aaaattgctg gcatgcaaca acgcaccatt gtgctgggca
      121 acttcatttt taacgtgctc aaaagcatcg taccaagctg tagttccaac aacaactggc
      181 aatgagtggt ttaaacaaaa ttgaatgttt gcaacagctg catcggggcg tgtaaactca
      241 attgccacat ccgcctcaac cgattttaac gattcaatgg gtgtgctttg gttgattttg
      301 gcaacaatgg aatgtcctcg ttctatggca atttcttcaa ttgcttttcc cattttaccg
      361 tatccaataa gtgctatttt catgttcgta atcaatcttt attttagtga taattgaagt
      421 gaaataccaa cagttggtgt tgccgaataa tccattggta tgactgctgg tctaattttc
      481 atcgataaat cttttgatac atcaaaattt acaaaatgtg cctcaacagc agcatcggca
      541 atttgcaata aatacaccaa tcccattcct aaaaacaata aatctcgacg tgtaattttt
      601 tggttataca attgcaaaat tccagcatcg tcgtaattac tgtacttttc aatgggggtt
      661 aatccatatt ctgtacggtt gatgtattct tgtttgagta ctttttgctc caaatgattt
      721 tttactgcaa aataaccagt aacgcccaaa cctgcataaa ttaaaggcac tttccaatat
      781 gcttttttct ttcctttggg cattgcaatg tgattgtaca cttgtccagc ccccggaagt
      841 gccgcagaaa gaatggctgc taattttaca gaatgttgtt tagtggaatc gcgttcaact
      901 tcttttttag tgttgatagg agccacttcc tgcgaaaaaa caggttgcaa cacagtccaa
      961 cacaaaaaaa gaaaaacgta tttcatttaa ttattcaata actcaataat tcggttcaaa
     1021 tcattttcgg agctaaattt taacacaatg gttccagagc catttgcggc ttttttaata
     1081 tccacttttg ttgccaaacg atcgctcaaa tgttctttga aaacgtattg tgattcgctt
     1141 attgtgttgc ttttagattt tttaacactt gcatcgctgt cagtatcttt atcagttgcc
     1201 gccaattgaa gctcgccttt taccaatgct tcggtgtcac gaaccgacaa tgaatgagct
     1261 accactttcg cataaatttc caattgtttt gcttcgtcac cagccgatac taacgaacga
     1321 gcatgtccca ttgaaataac attatcgcgc actccagctt gaatctcagc tggcaatttc
     1381 aataaacgca agaaattggt a
//
LOCUS       DAOQVQ010000569         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_130583_length_1401_cov_3.507429, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000569 DAOQVQ010000000
VERSION     DAOQVQ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130583_length_1401_cov_3.507429"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..510)
                     /locus_tag="PKN22_12020"
     CDS             complement(82..510)
                     /locus_tag="PKN22_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43477.1"
                     /translation="MKLPIILSLALVFLTCSTSCKKNKLHPVPSIPFDITIDINLPTY
                     SNLTGVGGYAYVTGGSKGIIVYRRSLGEFVAFDRHSPANEGACEKPLVTSEDNFLELI
                     DSCTYAKFSLYDGSPISGSEFGLRMYSTWFDGNSSLRIYN"
     gene            573..833
                     /locus_tag="PKN22_12025"
     CDS             573..833
                     /locus_tag="PKN22_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43478.1"
                     /translation="MLVLVCLGLLNLQACATNKIKKTPETVEEAEKELAKKRKKEAKL
                     NKKEAAAAKKRHWDLQSKAAKKSVKMNEKRMKKNKKRGVFMP"
     gene            870..1382
                     /locus_tag="PKN22_12030"
                     /pseudo
     CDS             870..1382
                     /locus_tag="PKN22_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
BASE COUNT          511 a          234 c          278 g          378 t
ORIGIN      
        1 acaagtgctt taatgagcga tttaatcaga gagaaagaaa aagaaaaatg gatgttaaaa
       61 gcttggttgg gcaaataaca attaattgta aattctcaaa gaagaattac cgtcaaacca
      121 agtggaatac atacgtaaac caaactcgga gcccgaaata ggtgagccgt cgtagaggga
      181 gaatttggca tacgtacaac tgtcaattaa ctccagaaaa ttatcttcgc tggtaaccaa
      241 tggtttttca cacgcacctt cattggcggg tgaatggcgg tcgaatgcca caaactcgcc
      301 taaacttcta cggtaaacaa tgataccttt ggaacctcct gtaacataag catagccgcc
      361 aactccagtt aaattactgt aggtgggtaa gttgatgtct attgtaatat caaaaggaat
      421 gctgggcaca ggatgcaatt tatttttctt gcacgatgtg gaacaagtga gaaaaactag
      481 tgctaaactt aaaataattg gtaatttcat tgcgttaaaa gtcgtattac aaaagtatga
      541 aatatttaca ttcaaagata aaaacggttt caatgcttgt tttagtatgt ttaggactac
      601 tgaatttaca agcatgtgca accaataaaa ttaaaaaaac tcccgaaacg gttgaagaag
      661 ccgaaaaaga attggcaaaa aagcgcaaaa aagaagccaa attaaataaa aaagaagccg
      721 ctgctgccaa aaaacgtcat tgggatttac aatcaaaagc ggctaaaaaa tcggtaaaaa
      781 tgaatgaaaa acgcatgaaa aagaataaaa aacgcggtgt ttttatgccg tagtttatta
      841 ttttcgcatt tttataagaa aaacattaaa tgtcacgcat tcccccgcat attattgacg
      901 atatcatgca aacatctcgc attgaagagg tgattggaga atttgtgaat ttaaaaaaag
      961 cgggttccaa cctcaaggga ttgtcgccgt ttacggatga aaaaacacct tcatttgtgg
     1021 tgtcgccttc caaacaaatt ttcaagtgct tttctacggg taaaggtgga actgtggtga
     1081 cgtttttaat ggaaaaagaa catttttcct accccgaagc actcaaatgg ttggccgaca
     1141 agtatggaat tcaacttcca gaagaaaaag aattaaccac tgaagagcaa tctgccattt
     1201 cggagcgtga aagtctttat atcatcaatg agtttgcaaa agaatatttt aagaacaaca
     1261 ttacttcaac cgatgaggga aaatcggtgg gctattctta ttttattgag cgtggatttc
     1321 gtcccgatat tattgaaaaa tttcaattgg gatattgcct caattcgggc aatgatttta
     1381 caaagctgca ttaaaaaaag g
//
LOCUS       DAOQVQ010000570         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_130586_length_1401_cov_3.394502, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000570 DAOQVQ010000000
VERSION     DAOQVQ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130586_length_1401_cov_3.394502"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1401
                     /locus_tag="PKN22_12035"
                     /pseudo
     CDS             231..1401
                     /locus_tag="PKN22_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
BASE COUNT          397 a          223 c          330 g          451 t
ORIGIN      
        1 tactattata aagtttctca atttcgccca tcaacaatgg tttcagcggt taagtattga
       61 acaagcagtg ttggagacgt taactgatga acgtattgaa aaccaatcca atgcaaatat
      121 ttggcaatat ttaacgtatt accgacagtt ggatagtagg agtaatgggt tttgatgaaa
      181 ttaaggtgtt ttgcatttaa gttattgatt atcatataac aacatgcagt tccttgcatt
      241 tttttgtgtt attggtcatt tttgctattt cattcatggg agcttttgaa attacattgc
      301 ctaactcatg ggtgaacaaa gccgatgcaa aagccgataa aggtggattg gtaggtattt
      361 tctttatggc attggtgttg gcattggtgt ctttttcatg taccggacca attgtaggaa
      421 ccttgttggt agaagccgca tcaactggtg gaatgacacc aattatcgga atgtttggtt
      481 ttggattggc attggccttg ccgtttgcct tgtttgctgc tttcccagga tggatgaact
      541 ctttgccaca atcgggaggg tggttaaact ctgtaaaagt ggtgttagga ttacttgaat
      601 tagctttggc attcaaattt ttatccaatg ccgatttggc atggcaagca cattggttgg
      661 aacgtgaagt atttattgct atttggattg ctgtatttgg gttcttaacc ttgtatttat
      721 tcgggttggt gttacttccg cacgattcaa aaacggataa aatttctgtt gggagagctt
      781 cattagggat gttaactctg gcatttgtta tttatttaat tccaggaatg tggggagctc
      841 cgttgaaatt aatcagtgca tttccaccac caatgcatta cagtgaatca cctcttggcg
      901 taggcgctac tgtaaatgaa tcggtttctg taaatgcggc gccagaagga acgcacttgg
      961 gacctcaaaa tatttatgtg tttcacgatt tggaaaaagc agaagcctat gctaaacaag
     1021 ttggaaaacc tttgttcatc gattttacag gtcacaattg tgtgaattgc cgtaagatgg
     1081 aacaatcggt ttggggtgaa cctggtgtca tagaaattct tcgtgataaa gtggtaattg
     1141 cgtcgttgta tgtggatgaa cgtacagatt tgcctaaaga agaacaggtg gaagttgttt
     1201 atccaaacgg gcgtaaagtg cagttgaaaa cagtgggcga taaatggaca gccaaacaag
     1261 taattgatta caaagtaact tctcaaccat attatgtgtt gaaaaatacg gatggaacgc
     1321 acattgacgt aggttcagca agctaccaaa cacacagcaa cccagctgat tttaaatcgt
     1381 ggttggagaa aggaatgaaa t
//
LOCUS       DAOQVQ010000571         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_130629_length_1401_cov_2.665676, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000571 DAOQVQ010000000
VERSION     DAOQVQ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130629_length_1401_cov_2.665676"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKN22_12040"
     CDS             <1..638
                     /locus_tag="PKN22_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43479.1"
                     /translation="KHKSFLLFLIIALTAICAFLSMAFNLNYYDTIHCTIALSVGGAF
                     GYFAYYNTFKINSLGKLKGWFLILLLGGIIVFSSSYILPKVLSFLLFSIATSLLIIYQ
                     INCASNYDLKRIPLIEKLGKYTYGFYMYHSIAIFLIYNLFTRFLNLDDNIYHTLITIP
                     LLSLGLTIVMGYYSYHYIEIKFLKIKDKLNNVKDKSIPSYVCNDDNSKLNG"
     gene            complement(644..>1401)
                     /locus_tag="PKN22_12045"
     CDS             complement(644..>1401)
                     /locus_tag="PKN22_12045"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS43480.1"
                     /translation="TIDRILLQNILYDVKLDELGNPQQAIAYAEGQVGIYSACYKLAM
                     LVTIILQAYRYAAEPFFFNNSKNTDSKKMYIKVMNYIIAALCVVFLGVALNLQLFKYF
                     IPNPAYWEGLKAVPLLLLANVFLGIYINQSIWYKLTGLTKYGAYIAIGGAFLTISINY
                     LFIPMYGYMASAWATMLVYAAMMVASYIWGQQHYPINYNVRKFVLYFGSALAVFFALH
                     YLALADGLLQFFVHNLAIALFVGGIVVVERGTK"
BASE COUNT          453 a          245 c          249 g          454 t
ORIGIN      
        1 aaaaacataa atcatttttg ttgtttttaa ttattgcttt aacagcaatt tgtgcgtttt
       61 taagtatggc tttcaatcta aattattatg acacaattca ttgcacaata gctttgtcgg
      121 tgggtggtgc ttttggctat tttgcctatt acaatacatt taaaattaat agtttgggca
      181 agctaaaagg atggtttttg atattattgc ttggggggat aattgttttt tcttcatcgt
      241 acattttacc taaagtatta agctttttat tattttcaat tgcaacatcg ctattaatca
      301 tttaccaaat taactgtgca tctaactatg atttaaaaag aattccgcta attgaaaaat
      361 tagggaaata cacttatgga ttctacatgt atcatagtat tgccattttt ttaatttata
      421 acctgtttac aagattctta aatctagatg ataatattta ccatacacta attacaattc
      481 cattattatc tttgggatta acaattgtga tgggatatta ttcttatcat tatattgaaa
      541 taaaattttt gaaaataaag gataagctga ataatgttaa agataagtct attccatctt
      601 acgtttgtaa tgatgataat tctaaattaa atggttgata ttgttacttc gttcctctct
      661 ccacaaccac aattccaccc acaaacagcg ctattgccaa gttgtgtaca aaaaattgga
      721 gcaatccatc tgcgagggcc aaatagtgga gtgcaaaaaa gacagccaat gccgaaccaa
      781 aatagagtac aaacttgcgc acgttgtagt taatcgggta gtgttgttgc ccccaaatgt
      841 aggaagccac catcatagcg gcatacacca acatggttgc ccaggcactt gccatatagc
      901 catacatcgg gatgaacaaa taattgatgg aaatggttaa aaacgcacca ccaatggcaa
      961 tgtaggcgcc gtatttggtg agtccggtga gtttgtacca aatgctttgg ttaatgtaaa
     1021 tgcccaaaaa tacgttggcc aacaacaaca acggaactgc ttttaaacct tcccaataag
     1081 cagggttggg aataaaatat ttgaacaatt gaaggttgag cgccacaccc aaaaacacca
     1141 cacacaaggc ggcaataatg tagttcatca ccttgatgta catttttttg ctatcggtat
     1201 tttttgaatt gttaaagaaa aacggctcgg cagcatagcg ataggcttgc aaaataatgg
     1261 ttaccaacat ggccaatttg tagcaagcgc tgtaaatacc cacttgcccc tcggcataag
     1321 caatggcttg ttgtgggttg cctaattcgt ccaattttac atcgtataga atgttttgca
     1381 acaaaatacg gtcgatggtt t
//
LOCUS       DAOQVQ010000572         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_131485_length_1395_cov_5.174627, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000572 DAOQVQ010000000
VERSION     DAOQVQ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131485_length_1395_cov_5.174627"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /gene="apaG"
                     /locus_tag="PKN22_12050"
     CDS             complement(<1..369)
                     /gene="apaG"
                     /locus_tag="PKN22_12050"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNS43481.1"
                     /translation="MSVAITEGVEIRVSVTFREDLSNIHMGSFFFNYDVFITNHNLFP
                     IQLLHRNWHIFDSMHDTNYVSGEGVVGEQPILRPHKTYNYMSGCELFSEIGYMKGFYT
                     FKNLNTEKLFKVEIPKFQLLY"
     gene            complement(434..1366)
                     /gene="queG"
                     /locus_tag="PKN22_12055"
     CDS             complement(434..1366)
                     /gene="queG"
                     /locus_tag="PKN22_12055"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNS43482.1"
                     /translation="MLLQSLHTKLIKDKAYELGFMFCGISKATFLEEEVKNFEEYLKE
                     NRHGKMQYLENYFDKRMDPTLLVDGAKSVVSLAVNYYPEKQQTDTSYKLSKYAYGVDY
                     HYVVKDKLKELFQFIQDEIGEVDGRMFTDSAPVLEKAWAAKSGLGWRGKNNLTIHPKA
                     GSFFFLAELILDLELEYDGPIKDYCGTCTKCIDACPTEALQPYKIDASKCISYLTIEL
                     KDELLPSEFQNKMENWMFGCDICQDVCPWNKFSKPHNEPAFYPHEKLFELNARDWEEL
                     TEEVYQEIFRKNPVKRAKYSGLMRNIAFLKDAKK"
BASE COUNT          432 a          263 c          222 g          478 t
ORIGIN      
        1 atataacaat tgaaacttcg gaatttctac tttgaacagt ttttctgtat tcaagttttt
       61 aaaggtgtaa aaacctttca tataaccgat ttcgctgaac aattcgcaac cactcatgta
      121 attgtaggtt ttgtgtggtc ttaatatcgg ttgttcacct accactcctt ctccactcac
      181 gtaattggtg tcgtgcatag aatcaaaaat gtgccagttt ctgtgcaaca attgaatggg
      241 aaataaatta tgattggtaa taaaaacatc gtagttaaag aaaaaagaac ccatgtgaat
      301 attggataag tcctctctaa aagttacgct aactcgaatt tctacacctt cagttattgc
      361 tacactcatc aacaatttat tttaaacttt agtgtaaaaa taaaataata tttgtgagtt
      421 aaacaattac aactcatttt tttgcatctt ttaagaaagc aatgttacgc atgagtccgc
      481 tatattttgc acgttttaca gggttttttc taaaaatttc ttggtaaact tcttctgtta
      541 actcttccca atcgcgagca tttaattcaa ataatttttc gtgcggatag aatgctggtt
      601 cgttgtgggg ttttgaaaac ttattccacg gacacacgtc ttggcaaatg tcgcaaccaa
      661 acatccaatt ttccattttg ttctgaaact cgctgggtaa aagttcatct ttgagttcaa
      721 tggttaaata agaaatgcat tttgaagcat caattttata gggctgtaaa gcttctgtag
      781 gacaagcgtc gatgcatttg gtgcaggtgc cgcaataatc ttttatcgga ccatcgtatt
      841 ccaattccaa atctaaaatc aactcggcta aaaagaaaaa agagcctgcc ttaggatgta
      901 tggttagatt atttttaccg cgccagccca atccactttt ggctgcccaa gctttttcta
      961 aaacaggagc cgaatcggta aacattcttc cgtccacttc accaatttca tcctgaataa
     1021 attgaaaaag ttcttttaac ttatctttta ctacgtaatg gtaatctacc ccataagcat
     1081 atttagaaag tttgtatgaa gtatcggttt gttgcttttc gggataataa ttcactgcta
     1141 atgacacaac tgatttagcg ccatctacca aaagagtggg atccatgcgt ttatcgaaat
     1201 aattttctaa atactgcatt tttccgtgac ggttctcttt taaatactcc tcaaagtttt
     1261 taacttcttc ttctaaaaaa gttgccttag aaatgccaca aaacataaaa cctaattcat
     1321 aagctttatc ttttatgagc tttgtatgca atgattgaag taacatgttc gattaaaaca
     1381 aaccgccttg ttgcc
//
LOCUS       DAOQVQ010000573         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_131655_length_1394_cov_3.581031, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000573 DAOQVQ010000000
VERSION     DAOQVQ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131655_length_1394_cov_3.581031"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKN22_12060"
     CDS             <1..550
                     /locus_tag="PKN22_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS43483.1"
                     /translation="KFNYKDLNISASYFYRLTTDAIDWVKDSLSAPWQPQNFQRIQLN
                     GITFSADYRLIKPKTMKDVSVIAGVSYTWLEPKIVRHPGQSAMYSHYSFDNMRNQLIG
                     RVNVGFLKYFNAGITGRFIERVSYKTYILLDASLSGTFAGVEVKLSCNNLLNVTYVEA
                     GATPLPGRWFNLGVKWQMWKKK"
     gene            complement(621..>1394)
                     /locus_tag="PKN22_12065"
     CDS             complement(621..>1394)
                     /locus_tag="PKN22_12065"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNS43484.1"
                     /translation="VLNQVATLFLEATRDIVPNWLIGTPDPSVAVGVACEPIRVEMVI
                     RGYLAGHAAREYKAGKRELCGVQLPEGMKENDKFPEPIITPASKADEGHDEDISREDI
                     IAKNIVPEHIYEQLEKYTRALFQRGTEIAAKQGLILVDTKYEFGIRNGEVILIDEIHT
                     PDSSRYFYADGYEERQAKGEPQKQLSKEFVREWLMANNFQGLDGQTMPEMPDSFVETI
                     TERYIELYERITGKTFVKAATDNIEERIYNNVTAFLKTM"
BASE COUNT          394 a          284 c          241 g          475 t
ORIGIN      
        1 taaattcaac tacaaagatt tgaatatttc tgctagttat ttctaccgct taactacaga
       61 tgcaattgac tgggttaaag acagtttaag cgctccttgg caaccacaaa acttccaacg
      121 tattcaattg aatggcatca cattctcggc tgattaccgt ttaatcaaac caaaaacaat
      181 gaaagatgtt agcgtaattg ctggtgtttc ttatacgtgg ttggaaccta aaattgttcg
      241 ccatccagga caatcagcca tgtattcgca ttattcattt gataacatgc gcaatcaatt
      301 aattggacgt gtgaatgttg gcttcttgaa atatttcaat gcgggtatta ctggtcgctt
      361 tattgaacgt gtttcctaca aaacttatat tttgttagac gcatcattgt cggggacatt
      421 tgctggtgta gaagtaaaac tttcatgcaa caacttgttg aacgttactt atgttgaagc
      481 tggtgcaaca ccacttccag gaagatggtt caacttgggt gtgaaatggc aaatgtggaa
      541 aaagaaataa gttgtttttt agaacatgaa aaaggcgaat gaactcaatc attcgccttt
      601 tttgataaat atcattcaac ttacattgtt tttaaaaacg ctgtaacatt gttgtaaatt
      661 cgttcttcga tgttatcggt tgctgctttt acaaacgttt ttcctgtaat gcgctcatac
      721 aattcaatgt aacgttctgt aatcgtttca acaaaagaat cgggcatttc tggcatggtt
      781 tgtccgtcta atccttgaaa attattagcc atcaaccact ctcttacaaa ttcttttgac
      841 aattgctttt gtggttctcc ttttgcttga cgttcttcat aaccatctgc ataaaaatag
      901 cgtgatgaat ctggcgtatg aatctcgtca attaagataa cttctccgtt tctaattcca
      961 aattcatatt tggtatccac taaaatcaac ccttgttttg cggctatttc tgttccacgt
     1021 tgaaacaatg ctctggtgta tttttccaat tgctcgtaaa tatgttctgg aacaatattt
     1081 ttagcaataa tatcttctct tgaaatatct tcatcgtgtc cttcatctgc tttagatgct
     1141 ggcgtaatga tgggctctgg aaatttatcg ttttctttca tcccctcagg caattgaaca
     1201 ccacacaatt cacgttttcc tgctttgtat tcacgtgctg cgtgtcctgc caaataccca
     1261 cgaataacca tttcaactcg aatcggttca caagccacac caactgccac cgatggatct
     1321 ggcgttccta tcaaccaatt gggaacgata tcgcgcgtag cttccaaaaa caaagttgct
     1381 acttggttta aaac
//
LOCUS       DAOQVQ010000574         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_135205_length_1372_cov_2.580866, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000574 DAOQVQ010000000
VERSION     DAOQVQ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135205_length_1372_cov_2.580866"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKN22_12070"
     CDS             complement(<1..312)
                     /locus_tag="PKN22_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43485.1"
                     /translation="MRSLFFLPFFGLITFYSCSQTSYEHEKIEVVQNNEYNVLIDASK
                     LFSERWSELPQAKFWMEIMKLPPDSCIINVASNRRILEVTSIKKWNSQTEVQKSNYRD
                     SL"
     gene            complement(449..>1372)
                     /locus_tag="PKN22_12075"
     CDS             complement(449..>1372)
                     /locus_tag="PKN22_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS43486.1"
                     /translation="PSEVTPYTAYLLGEVIKESGMPNGVLNILHGLGATVGDAIVKHP
                     AIKAISFTGGTKTGEYIARTAAPMFKKMSLELGGKNPNIIFADCDFDEMLKITVRSSF
                     ANQGQICLCGSRIFVERPIYEKFKTAFVEKVSRMKVSYPSDEQANMGALVSKPHLEKV
                     LSYIELAQQEGGTVLTGGERVQMEAPYENGYFLRPTVIEGLSYDCRTNQEEIFGPVVT
                     ITPFDTEEEVLMMANSVKYGLSATVWTTNLQRAHRVADEIEAGIVWINDWLVRDLRTP
                     FGGAKASGVGREGGWDALEFFTEPKNVFIKY"
BASE COUNT          421 a          301 c          217 g          433 t
ORIGIN      
        1 taggctatcg cgataattgc ttttttgcac ttcggtttga ctgttccatt tttttatgga
       61 tgtaacttcc aatattcttc gattagatgc cacgttgata atgcatgaat cgggtggtaa
      121 tttcataatc tccatccaaa attttgcttg aggcaactca ctccatcgct cagaaaaaag
      181 ctttgaagca tcaatcaaca cattgtattc attgttttga acaacttcaa ttttttcgtg
      241 ttcataggat gtttgactgc acgaataaaa cgttatcagt ccaaaaaatg gaagaaaaaa
      301 taaggagcgc atggttttat agttaaagtt tcgatgaaat tacaaaaaac aagtgattat
      361 tcaaagtaaa atgtgcagaa ttttacacaa tgttatttta ataagttaca tcactcgcaa
      421 attcaaccgt aaaacaaacg ctcttgaatc aatattttat gaacacgttt ttaggctctg
      481 taaagaattc caaggcatcc caccctcctt ctctaccaac tccagaggct tttgctccac
      541 caaaaggcgt acgtaaatcg cgtaccaacc aatcgtttat ccacacaatt cccgcttcaa
      601 tttcatcggc tactcggtgc gcacgttgca aattggttgt ccaaaccgtt gcgctcaatc
      661 catattttac cgaattagcc atcatcaaca cttcttcttc ggtgtcaaaa ggtgtaatgg
      721 taacaacggg tccaaaaatt tcttcttggt tagtgcggca atcgtagctc aatccttcaa
      781 tcaccgttgg acgcaagaaa taaccatttt cataaggtgc ctccatttgc acacgttcac
      841 cacctgttaa aacagttcca ccttcttgtt gtgctaattc aatataagaa agcacttttt
      901 ctaaatgcgg ttttgaaacc aaagcaccca tatttgcttg ctcgtcggat ggataagaaa
      961 ccttcattct acttactttt tctacaaaag ctgttttgaa tttttcataa attggtcgct
     1021 ctacaaaaat tcgcgaacca cacaaacaaa tttgcccttg attagcaaaa gaagaacgaa
     1081 cagtaatctt taacatttca tcaaaatcgc aatctgcaaa tatgatatta gggttttttc
     1141 cgcccaattc taaactcatt ttcttgaaca ttggagccgc tgttcgagca atatattctc
     1201 ccgtttttgt tccaccagta aacgaaattg ctttgattgc tggatgtttt acaatggcat
     1261 caccaaccgt agcacccaag ccatgcaaga tgtttaaaac accattgggc atccccgatt
     1321 ctttaataac ttcacctaat aaataagctg tataaggcgt aacttctgat gg
//
LOCUS       DAOQVQ010000575         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_135226_length_1372_cov_2.311314, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000575 DAOQVQ010000000
VERSION     DAOQVQ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135226_length_1372_cov_2.311314"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PKN22_12080"
     CDS             <1..1310
                     /locus_tag="PKN22_12080"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNS43487.1"
                     /translation="VTLSEILNLHEYARNVVNKSIDVFTTSELAEELLKMDLAPAKLD
                     IGKLASEWLEEQDNYASKLAFLKAKLAGFETSNNDSIEPRDVVLYGFGRIGRLAAREL
                     IKQAGKGQQLRLRAIVTRGDSDQDIIKRASLLKNDSVHGAYKGTVSVDLEKKAIIING
                     QIVRMISANNPEDVDYTAYGINNALVIDNTGVFTNREALGRHLQAKGVSKVILTAPGK
                     EIPNIVYGINQGTLDVENETIYSAASCTTNAISPILKVINDQFGIERGHVETIHAYTN
                     DQNLLDNMHKKPRRGRSAAVNMVITSTGAGNAVTKVIPELKDKLTANAVRVPTPNGSL
                     AMLNLELKKGTTVEEVNALVRKASLEGELVNQIFYSYDPEMVSTDIIGNTCCSVYDSN
                     ATIVSPDGKSVVLYTWYDNEFGYTKQVIRLAKYVAKVRRNIYY"
BASE COUNT          473 a          223 c          284 g          392 t
ORIGIN      
        1 acgtaactct ttctgaaatc ttaaacttgc acgaatatgc aagaaatgtt gtcaacaaat
       61 cgattgacgt gttcacaact tctgaattag cagaagaatt attgaaaatg gatttggctc
      121 cagcaaaatt ggacattggt aaattggcta gcgaatggtt ggaagaacaa gataactatg
      181 cttctaaatt ggcattctta aaagctaaat tagctggttt tgaaacgagc aataacgaca
      241 gcatcgaacc aagagatgtt gtgttgtatg gttttggacg catcggtcgt ttggcagctc
      301 gcgagttaat caaacaagct ggtaaaggtc aacaattgcg tttgcgtgct attgtaacaa
      361 gaggagattc tgatcaagac atcatcaaac gtgcttcttt gttgaaaaac gactctgtac
      421 acggagcata caaaggtacg gttagcgtag atttagagaa aaaagcaatc atcatcaacg
      481 gacaaattgt gcgcatgatt agtgcaaaca acccagaaga tgtggattat acagcatacg
      541 gaatcaacaa tgcgttggta attgataaca ctggtgtatt caccaaccgt gaagcattgg
      601 gtcgccactt acaagcaaaa ggtgtttcta aagttatttt gactgcgcca ggaaaagaaa
      661 ttccaaacat tgtttatggt atcaatcaag gaactttgga tgtagaaaac gaaacaattt
      721 attcggcagc gtcttgtaca accaatgcta tttctccaat cttgaaagtt atcaacgatc
      781 aatttggaat tgaaagaggg cacgttgaaa caattcacgc ttatacaaac gaccaaaatt
      841 tgttggataa catgcataaa aaaccaagaa gaggtcgttc tgctgctgta aacatggtca
      901 tcacttctac aggtgctggt aatgcagtaa caaaagtaat tcctgagttg aaagacaaat
      961 tgacagcaaa tgcggtacgt gtaccaactc ctaacggttc tttggcgatg ttgaacttgg
     1021 aattgaaaaa aggaacaacc gttgaagaag taaacgcatt ggtgcgtaaa gcatcgttgg
     1081 aaggtgaatt agttaatcaa attttctatt catacgatcc tgaaatggta tcaacagata
     1141 ttattggtaa tacttgttgt tcggtttacg attcaaatgc aacaattgta tctccagatg
     1201 gaaaaagcgt tgttttatac acttggtatg acaatgagtt tggttacaca aaacaagtta
     1261 ttcgtttagc gaaatacgta gcaaaagtta gaagaaatat ttattattaa tctaactatt
     1321 cagcttttag ttgaacgtaa atacatacta aaatggttgt tttcaagttg aa
//
LOCUS       DAOQVQ010000576         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_135343_length_1371_cov_2.901976, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000576 DAOQVQ010000000
VERSION     DAOQVQ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135343_length_1371_cov_2.901976"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKN22_12085"
     CDS             complement(<1..749)
                     /locus_tag="PKN22_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS43488.1"
                     /translation="MRPTKLSSKTTAFSLKTKNSETISVHFGENHQPVFLKENKDTVE
                     LEYTIKSVVFKNQSGKLLNGWEIMPKAPVQPVATLVHFHGNAGSLLHQFNAVTPLVKE
                     GFAVFMFDYSGFGFSEGRPTRKNVLKDGNAALDYVLTNFSPAKKIVIYGQSLGGHLAG
                     VVGTQRQKDIDALVIEGAFSSHKTVAQAFAGGFGKAFTKEMYSAKDSIAHYHKPLLII
                     HSTEDEVIPFEMGKEIFNKANHPKEFLEVKE"
     gene            complement(841..1098)
                     /locus_tag="PKN22_12090"
     CDS             complement(841..1098)
                     /locus_tag="PKN22_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNS43489.1"
                     /translation="MTLNKEELTKKVNEVLTQLRPYLHEDGGDMELVKITDDGIAHVR
                     LIGACSDCHMSTMTLKAGLEAQVKKIAPEIVSVIAFDDAEA"
     gene            complement(1095..>1371)
                     /locus_tag="PKN22_12095"
     CDS             complement(1095..>1371)
                     /locus_tag="PKN22_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNS43490.1"
                     /translation="AWFTPAELPENKYYIFGRDGAKNLAEGMGVVLLGQIPLVQSIRE
                     SGDVGKPAVFQENTVVSQAFDDLCKVFVEEVEKAKEQKSKKKHNAVV"
BASE COUNT          397 a          299 c          208 g          467 t
ORIGIN      
        1 cattctttca cttctaagaa ctcttttgga tgattcgcct tattaaaaat ttcttttccc
       61 atttcaaaag gaatcacttc atcttctgtg ctatgaatta ttagcaatgg cttgtggtag
      121 tgagcaatag aatctttggc gctgtacatt tcttttgtaa atgctttacc aaaaccacct
      181 gcaaatgctt gtgcaacagt tttatgcgaa gaaaaagcac cttcaatcac aagtgcatca
      241 atatcttttt gtcgttgcgt ccccactaca ccagccaaat gtcctcccaa cgattgcccg
      301 taaataacaa ttttctttgc aggagaaaaa ttggtcaaca cataatccaa agcggcattt
      361 ccatctttca acacattttt tcgggttggg cgaccttcag aaaagccaaa tccactgtaa
      421 tcaaacataa agacagcaaa accttctttc actaatggtg ttacagcatt aaattggtgt
      481 aacaacgaac ctgcattgcc atgaaaatgg acgagtgtgg ccactggttg aaccggtgct
      541 ttgggcatta tttcccatcc attcagcaat tttcccgatt gatttttgaa aaccacactt
      601 ttaatggtgt actccaactc aactgtatct ttattttctt ttaaaaatac aggttggtga
      661 ttttctccaa aatgaacgga tattgtttca ctgtttttag tcttcaatga aaaagcagtt
      721 gtttttgaag aaagttttgt aggacgcaaa aatatagaat tcagtgcaca cgattgcaaa
      781 aagagcattg cagcaaataa ccacaatact ctttttaatt catatttcac agctttcaac
      841 ttatgcttct gcatcgtcaa aagcaatcac agaaacaatt tcaggagcaa ttttctttac
      901 ttgtgcttct aaacctgctt tcaacgtcat tgttgacatg tggcaatcac tgcatgcacc
      961 aattaaccgt acatgtgcta taccatcatc cgtaattttt accaattcca tatcccctcc
     1021 atcttcatgt aagtagggac gtaattgcgt taaaacttcg tttacttttt ttgttagttc
     1081 ttctttattc agtgtcatac tactgcgtta tgttttttct tcgatttttg ttcttttgct
     1141 ttttcaacct cttccacaaa tactttgcat aaatcatcaa atgcttgact taccactgta
     1201 ttttcttgga aaacagctgg tttaccaaca tcgccagatt cacgaatgct ttgtaccaat
     1261 ggaatttgac ctaaaagtac cactcccatt ccttcagcta agtttttagc gccatcgcga
     1321 ccaaaaatat agtatttatt ttcaggtaat tcagccggtg taaaccatgc c
//
LOCUS       DAOQVQ010000577         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_136961_length_1361_cov_2.787902, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000577 DAOQVQ010000000
VERSION     DAOQVQ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136961_length_1361_cov_2.787902"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKN22_12100"
     CDS             <1..402
                     /locus_tag="PKN22_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNS43491.1"
                     /translation="AVQMLHDSSDINEERELFIELMRSCYKKDVNKMLDWCENMDQYN
                     KEFQKNFLMYCLYMMRQSLMKNYTDDVLMRASNEEAEFLKNFARFISGNNIADFNDMF
                     SKAYYYIERNANTKLIFTNICFNVMRYIHAA"
     gene            467..>1361
                     /locus_tag="PKN22_12105"
     CDS             467..>1361
                     /locus_tag="PKN22_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNS43492.1"
                     /translation="MKSILVLGAGLSASSLIRYLLKESEKYDWKIQITDQDIEKVKAK
                     INNHPNGIALNFDALNKDERRKHIQQVDLVISMLPAIFHVEVAKDCIDFKKDLVTPSY
                     VSPEMRALESAAKEAGITILNEIGVDPGIDHMSAMKIIDEIKEKGGKIDCFKSFCGGL
                     IAPESDDNPWNYKFTWNPRNVVLAGAGGAAKFIRNGQYKYIPYHHLFSRLDYLSVEGY
                     GDFTGYANRDSLSYRSIYGLEGIPTIYRGTLRRPDFCEAWDIFVQLGMTDDSYVLTDL
                     DVCSPREFLNTYLPYHPTRSVE"
BASE COUNT          447 a          225 c          260 g          429 t
ORIGIN      
        1 gcggtacaaa tgttacatga ttctagtgat attaacgaag aacgcgaatt gtttatcgaa
       61 ttaatgcgct cgtgctataa aaaagacgta aacaaaatgt tggattggtg cgaaaacatg
      121 gatcaataca ataaagaatt tcaaaaaaac tttctgatgt attgcctcta catgatgcgc
      181 caaagtttaa tgaaaaacta taccgatgac gtgctaatgc gtgcatccaa tgaagaagct
      241 gagtttctga aaaactttgc tcgttttatt tctggaaaca acattgccga tttcaacgat
      301 atgtttagca aagcctatta ctacattgaa agaaatgcaa atacaaaatt aatcttcaca
      361 aatatttgtt ttaacgtaat gcgttacatt catgcagcat aatttttagt agttgaatgt
      421 tattttattt gttaattcat tacatttgtg ttaaaaatta gctaaaatga aatcaatatt
      481 agttcttggt gcaggtctat cagcctcttc gttaattaga tacctattaa aagaatcgga
      541 aaaatacgat tggaaaattc agattaccga ccaagacatt gaaaaagtta aggctaaaat
      601 caataatcat cccaacggaa ttgcgctcaa ttttgatgca ttaaacaaag acgaacgtag
      661 aaaacacatt caacaagttg atttagtaat ttcaatgtta cctgcaattt ttcatgtgga
      721 agttgctaaa gattgtattg atttcaaaaa ggatttggtt actccttcct acgtatctcc
      781 agaaatgcgt gcgttggaaa gtgctgctaa agaagccgga attactattt tgaatgaaat
      841 aggtgtagat ccaggcatcg atcacatgag cgccatgaaa atcattgatg aaatcaaaga
      901 aaaaggtggt aaaattgatt gcttcaaatc gttttgtggt ggattaattg ccccagaatc
      961 cgacgataat ccgtggaatt acaagtttac gtggaaccct cgcaatgtgg tattggcagg
     1021 tgcaggtggt gctgcaaaat ttatacgcaa cggacaatat aaatacattc cttaccacca
     1081 tttattttcg cgtttagatt atttatcggt tgaaggatac ggtgatttta caggttatgc
     1141 caatcgcgac tcgctttctt atcgcagcat ttatggtttg gaaggcatcc ctactattta
     1201 tcgtggaaca ttgagacgtc cagatttttg tgaagcttgg gatatttttg ttcagttggg
     1261 aatgaccgac gatagttatg tactaaccga tttggatgta tgttcgccaa gagaattttt
     1321 gaacacctat cttccctacc acccaactcg ttctgtagaa g
//
LOCUS       DAOQVQ010000578         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_137754_length_1356_cov_4.758647, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000578 DAOQVQ010000000
VERSION     DAOQVQ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137754_length_1356_cov_4.758647"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKN22_12110"
     CDS             <1..213
                     /locus_tag="PKN22_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS43493.1"
                     /translation="HRFGITRHRNKRSKAAIQSQVLAIDGIGEKTQIALMKKFKTVSR
                     IKAASLEELTEVIGKAKATLVKNALQ"
     gene            406..>1356
                     /locus_tag="PKN22_12115"
     CDS             406..>1356
                     /locus_tag="PKN22_12115"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNS43494.1"
                     /translation="MTQLTTSVQSPTNLVQNVLLGDPGITVSNISFLGAPSAIGSFDA
                     TGTNLGIEKGIIMTTGTIIGNTNGPIGPNNSASAGYDNNTAGYQLLTSIVGKQTFNAS
                     VLSFDFSTCSDSIEFRYVFGSEEYPEYVGSQFNDVFGFFISGPGFSGQQNIARLPNGS
                     VVAINNVNNGNSSPAQGVPVTGPSNPQYFVGNGNGSQAPYNASSIYIQYDGFTKVLTA
                     KAKVQCGQTYHLTLAIADVGDGVWDSGIFLEAKSFKANDPLKVTHTIVNPIPNTSNTL
                     AEPCSQAKIKFERTNCNINSPMVINITPSGTTNYQIAGQTS"
BASE COUNT          436 a          262 c          257 g          401 t
ORIGIN      
        1 caccgatttg gaattacacg tcaccgtaac aaacgaagca aagccgccat tcaatcgcaa
       61 gttttggcca ttgatggtat tggcgaaaaa acgcaaattg cattaatgaa aaagtttaaa
      121 actgttagcc gtatcaaagc agcttcgttg gaagaattaa ctgaagttat tggaaaagca
      181 aaagccactt tggtaaaaaa tgcattacag taaagtaaat tatttcaacc gtttaggtgt
      241 caagaggtac ttttaaatga aaaaagcggc tatttaacac tttttttgca tttcggcaca
      301 caaattgaaa gcaaataagc aaacgtttta tgtatttttg cgtataatct aataatgaag
      361 tacttatttt ccatattaac tttttgttgt tctatttttt atggaatgac tcagcttacg
      421 accagtgtgc agagtccaac caatttagtg caaaatgttt tattgggaga tccaggaatt
      481 actgtaagca acatttcgtt tttaggtgcc ccaagtgcca ttggtagttt tgatgctaca
      541 ggcaccaact tgggtatcga gaaaggaatc atcatgacta caggtaccat tattggaaat
      601 accaacggac caataggtcc taacaactct gcaagtgctg gatacgacaa caacacagcg
      661 ggatatcaat tattgacctc catagttgga aagcaaacat ttaatgcatc tgttttatct
      721 tttgatttct ctacttgttc tgattccatt gaatttaggt atgtttttgg ttcggaagaa
      781 tatcctgaat acgttggttc acaattcaac gacgtttttg gcttctttat ttctggccct
      841 ggtttttctg gacagcaaaa tattgcacgt ttacccaacg gtagtgtcgt tgctatcaac
      901 aatgtcaaca atggaaatag ttccccagca caaggagttc cagtaactgg tccgagtaat
      961 ccgcaatatt ttgtcggcaa cggaaatggt agtcaagctc catacaatgc aagtagtatt
     1021 tacattcaat acgatggttt taccaaggtt ttaacggcta aagcaaaagt gcagtgtgga
     1081 caaacctatc accttacact tgcaattgcc gatgtaggag atggtgtgtg ggattctgga
     1141 atctttttag aagctaaaag ttttaaggca aacgacccac taaaagttac ccataccatt
     1201 gttaacccaa ttccaaacac ctccaatacg ttggcagaac cttgtagtca ggcaaaaata
     1261 aaatttgagc gcaccaactg caacatcaac tcgccaatgg taatcaacat aacaccttct
     1321 ggaacaacga attatcagat tgccggtcaa acaagc
//
LOCUS       DAOQVQ010000579         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_137915_length_1355_cov_3.759231, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000579 DAOQVQ010000000
VERSION     DAOQVQ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137915_length_1355_cov_3.759231"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKN22_12120"
     CDS             <1..258
                     /locus_tag="PKN22_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43495.1"
                     /translation="KVDIVAPGTFSYKATSPITGQVFTVNGDGTYTWVCNLDDKPTQG
                     TYYLQPGNYKVVYRSKKIKSTLYTTEKEFRIYSNKITSLNF"
     gene            262..1215
                     /locus_tag="PKN22_12125"
     CDS             262..1215
                     /locus_tag="PKN22_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNS43496.1"
                     /translation="MIEIEVKGKKDTRSGFGEGLLELGRTNPDVVALCADLTGSLKMD
                     AFQKECPERFFQVGIAEANMISIAAGMTIGGKIPFTGTFANFSTGRVYDQIRQSVAYS
                     NKNVKIAASHAGLTLGEDGATHQILEDIGMMKMLPHMTVIVPADFNQTKQATIACAKH
                     DGPVYLRFGRPSVPIFVKEDAEFVIGKADTIIEGTDVTIIACGHLVWKSIEAAIELEK
                     QGISAEVINMHTIKPLDEAAVLKSIAKTKCVVTAEEHMYHGGLGDSIAQLLARTTPTP
                     QEYVAVNDTFGESGTPDELMAKYHIDTPDVVAAALKVIKRK"
BASE COUNT          471 a          231 c          270 g          383 t
ORIGIN      
        1 aaggtggata ttgtagcgcc tggaacattt tcatacaaag ctacaagccc aattacaggt
       61 caagtgttta cagttaacgg tgatggaact tatacatggg tgtgtaactt ggacgataaa
      121 ccaactcaag gaacctacta tttacaacct ggaaactata aagtagtgta tcgtagcaaa
      181 aaaattaaaa gcacgcttta cacaacagaa aaagaattta gaatttactc aaataaaata
      241 acctcattaa atttttaagc aatgattgaa attgaagtta agggaaaaaa agatacccgt
      301 tctggttttg gagaaggtct tttagaatta ggtagaacca acccagatgt tgttgcattg
      361 tgcgccgact taacaggttc gttaaaaatg gatgcttttc aaaaagaatg tccagaacgt
      421 tttttccaag tcggtattgc cgaagcaaac atgattagca ttgcagctgg tatgaccatc
      481 ggtggaaaaa ttccatttac tggaacgttt gccaatttct caactggacg tgtgtacgac
      541 caaattcgtc aatcggttgc ttattctaac aaaaacgtaa aaattgcagc atcgcatgca
      601 ggtttaacat tgggtgaaga cggtgcaact caccaaattt tggaagacat tgggatgatg
      661 aaaatgttgc ctcacatgac ggtaattgtt cctgcagact tcaatcaaac aaaacaagca
      721 accattgctt gtgcaaaaca cgatggtccg gtttacttgc gttttggtcg cccaagtgtg
      781 cctatttttg ttaaagaaga cgctgaattt gttatcggaa aggccgatac tattattgaa
      841 ggaacggatg taaccatcat tgcttgtggt cacttggttt ggaaatcaat tgaagcagca
      901 atagaattag aaaaacaagg gatttctgct gaggttatca acatgcacac catcaaacca
      961 ttggatgaag cagctgtgtt gaagtcaatt gctaaaacaa aatgcgtggt tacagcagaa
     1021 gaacacatgt atcacggagg attgggcgat tctattgcac aattattggc aagaacaaca
     1081 ccaacacctc aagaatacgt agcggtaaac gatacttttg gagaatctgg aacaccagat
     1141 gaattgatgg caaaatacca catcgataca ccagatgttg ttgcagcagc tttgaaagta
     1201 attaaaagaa aataaaaaat tcaaaccgaa tagtaaaagg ttgtcagaat ttggcaacct
     1261 tttatttttt tattattttt gattataatt ttttaattaa agctgaattc aagtcacaaa
     1321 tgcaacttct gagttaaaca ataatttttc catta
//
LOCUS       DAOQVQ010000580         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_139750_length_1344_cov_4.261443, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000580 DAOQVQ010000000
VERSION     DAOQVQ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139750_length_1344_cov_4.261443"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..472
                     /locus_tag="PKN22_12130"
     CDS             236..472
                     /locus_tag="PKN22_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNS43497.1"
                     /translation="MRKFELLTDFIQFLESELLLDKGEVQATSQLRQLRTWSSLNALI
                     LISRINDETAIVLTASDLAKCITVKDLHQHLSNQ"
     gene            472..>1344
                     /locus_tag="PKN22_12135"
     CDS             472..>1344
                     /locus_tag="PKN22_12135"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNS43498.1"
                     /translation="MALSKHTGSEIVGIVTVLPKTVEDNLELEMLSTDERKALVNHTG
                     IRYRRIASKQTTIKDLFEKAASQLLEKLAWENNQIDALICVTQTPQMVIPSVACRLHG
                     DLNLSSNTMCFDIVSGCSGFVYGLQVVYSMLASLGRDARAILCCGDISTQLIDANDKS
                     VRPIFSDAASAIAIEYKKGDKNKCGFFNLQTDGSGQHAIEITHQHNDKMQLNGIDVFG
                     YSVKLVPQNIQQLLDFAQHSIIFPDLFVLHQANQLINESIRKKLNIPEDKVPYSITEF
                     GNTASASIPLTLSLA"
BASE COUNT          446 a          250 c          239 g          409 t
ORIGIN      
        1 ttgtaaaaat tccacatata cataagataa aatccaatat tacatagtct aaattcaatt
       61 tcaacactat ttgatgaata cacaaaacac ctcgcaaaaa cgcaccaatg aaaccatcgt
      121 gagttggcaa gatgcagtct taattggtta gtgcttgcca gttctacttt ttctaaaaag
      181 caacgtttct tctgaaaaaa cgtttagttt taagatagtt tattacattt gttctatgcg
      241 aaaatttgag ctacttacag atttcatcca atttttagaa agtgaactat tgctcgacaa
      301 aggagaagtt caggcaacaa gtcaattgcg ccaattgcgt acttggagct ctttgaatgc
      361 tttgatatta atttctagaa ttaacgatga aactgccatt gttttaacag caagcgattt
      421 ggcaaaatgt atcacagtga aagatttaca ccaacatcta tctaaccaat aatggctctt
      481 tccaaacata caggatctga aattgtaggt atcgttacgg tattgcccaa aacagtagaa
      541 gacaatttgg aattggaaat gttatcgacc gatgaacgaa aagctttggt taatcacact
      601 ggaattcgtt accgtagaat tgcctccaaa caaacaacta tcaaagattt atttgagaaa
      661 gcagccagtc aattacttga aaagttggca tgggaaaaca accaaattga tgcgcttatt
      721 tgtgtaactc aaactccaca aatggtaatt ccttccgttg cttgtcgctt gcacggcgat
      781 ttaaaccttt catccaatac aatgtgtttt gatattgtat cgggttgttc ggggtttgtt
      841 tacggacttc aagttgttta tagcatgctt gcaagtttag gcagagatgc acgcgcaatt
      901 ctttgttgtg gcgatatttc tacacaatta attgatgcta acgataaaag tgtacgtcct
      961 atcttttcag atgcagcatc ggctattgct attgaataca aaaaaggcga taaaaacaaa
     1021 tgtggatttt tcaacttgca aacagatggc tcgggtcaac atgcaataga aattacacac
     1081 cagcacaacg ataaaatgca actcaacggc attgatgttt ttggctattc ggtgaagttg
     1141 gtgccacaaa atattcaaca attattggat tttgcgcaac attcgataat ttttcctgat
     1201 ttatttgtgt tgcatcaggc aaatcaatta atcaatgagt ccatccgaaa aaaactaaac
     1261 atccccgaag acaaagtgcc ttattccatc acagaatttg gaaacacagc ttcggcaagc
     1321 ataccgctaa cattgagttt ggct
//
LOCUS       DAOQVQ010000581         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_139796_length_1344_cov_2.602793, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000581 DAOQVQ010000000
VERSION     DAOQVQ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139796_length_1344_cov_2.602793"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PKN22_12140"
     CDS             complement(<1..1248)
                     /locus_tag="PKN22_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS43499.1"
                     /translation="MFNKFIDRPVLSTVISVIIVILGILGLVTLPISQYPEIAPPTVR
                     VSASYQGANAETLMKSVVVPLEEQINGVEDMTYMTSTSSNNGTASISIYFKLGTNADM
                     AAVNVQNRVARALPLLPQEVTKAGVVTSKRQSDNILIFDLNSSNPEYDMTFLQNYAEI
                     NILPRIKRVNGVGEAMIFSQNNYSMRIWLRPDVMASYGLVPSDIAAALAEQNLEAAPG
                     QLGENSNQSFQYVLKYKGRLKSVEEFEDIVINSTENGEILKLGDVARVELGSQTYAGM
                     TYSNGKQGVGIAIAQTSGSNAQDVIKGVMKVIDEVKVNFPQGIGVDILTNANDFLEAS
                     ISKVIQTLIEAFILVFLVVFIFLQDWRSTIIPAISVPVAIIGTFFFLSLFGFTINLLT
                     LFALVLAVGIVVDDAIVVVEAVHA"
BASE COUNT          421 a          279 c          243 g          401 t
ORIGIN      
        1 ggcgtgcacc gcttcaacga cgacaatggc atcatccacc acaataccaa cagccaacac
       61 caatgcaaat aaggttagca agttaatggt aaacccaaat aaacttaaaa agaagaacgt
      121 accaatgatg gcaactggaa ccgaaattgc aggtatgatg gtagaacgcc aatcttgcaa
      181 aaagatgaat actaccaaaa ataccaatat aaatgcttca attaaggttt gaattacttt
      241 tgaaatggat gcttctaaga aatcgtttgc gttggtaaga atatcaacac caattccttg
      301 tggaaagttt actttaactt catcaataac tttcattaca cctttgatta catcttgtgc
      361 attggagcct gatgtttgtg caatggcaat acccacacct tgtttaccgt tagaataggt
      421 cattccggca taggtttgcg aacctagttc aacacgagca acatcgccta attttaaaat
      481 ttcaccattt tcggtagaat taataacgat gtcttcaaat tcttctacac tttttaaacg
      541 tcctttgtat tttaagacat attggaacga ttgattactg ttttctccca attgtccagg
      601 agcagcttct aagttttgct cggccaatgc agcagcaata tcacttggaa ctaatccata
      661 acttgccatc acatcggggc gcaaccaaat acgcatagag tagttgttct gactaaaaat
      721 catggcttca cccacaccat ttacacgctt aatacgcggc aaaatgttga tttcagcata
      781 gttttgcaaa aacgtcatgt cgtattctgg gttgctactg ttcaagtcaa aaatcaaaat
      841 attatcactc tgacgtttag aggtaacaac tccagctttt gtaacttctt gcggcaataa
      901 tggtaatgca cgagcaacgc ggttttgcac gtttacggca gccatgtcgg cattagttcc
      961 caatttaaaa taaatactga tggaagctgt accattgtta cttgacgttg aggtcatgta
     1021 ggtcatgtct tccacaccat tgatttgttc ttccaatggg acaacaacag atttcatcaa
     1081 ggtttctgcg tttgctcctt gatagctggc actcactcta acggttggcg gcgcaatttc
     1141 tgggtactgt gaaataggta aagttaccaa tcctaaaata cctaggataa caattattac
     1201 cgaaataact gtagaaagta cagggcggtc tatgaattta ttaaacataa ggcattaaat
     1261 tttagaattt taaaacgctg tctgcaggca caactgtagg tttaattttt gttccttcgg
     1321 caacaatacc gatgccttca atga
//
LOCUS       DAOQVQ010000582         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_140084_length_1342_cov_4.165501, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000582 DAOQVQ010000000
VERSION     DAOQVQ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140084_length_1342_cov_4.165501"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..684
                     /locus_tag="PKN22_12145"
     CDS             175..684
                     /locus_tag="PKN22_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43500.1"
                     /translation="MNDFLDSDEERDRRPVFLTVVGILSLVNISWSLIQNITSFLSGP
                     LTEEGMEAYRLEMAKSLSTVQDTGVVWAEDLIQSAMNMVESINVNHTMNLLSSTVILI
                     IGLAAVIMMLKGRKIGFHGYIIYSFLASVQIYLFVTPSLLPNIMVIFSLLASGLFILF
                     YGLNLKWMK"
     gene            complement(760..>1342)
                     /locus_tag="PKN22_12150"
     CDS             complement(760..>1342)
                     /locus_tag="PKN22_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNS43501.1"
                     /translation="QGCHSSYTHNFTIGSELTVQFQASVDCFQTVTLTATPANYASYN
                     WLDGTTAIGTGNPLVYTAQTAGDHTYALTAADANGCQATTSAIVNVPTGVTAENFNIP
                     NIITPNGDGVNDFVVFPLMDLECINYKVTFVNRWGHPIFEASKDNPIFYGKDKKGTEL
                     SDGVYFYKIVSDDFNCKEEPYKSKCHGFITIQK"
BASE COUNT          404 a          222 c          262 g          454 t
ORIGIN      
        1 aatcaatgcc gcacaagggt tggagtttag acgtccgttg aaaagttctt caatcatcga
       61 acaattgcat caagaatacc gcaaagtggt accttttatt gatactgaca cctatttcca
      121 tccacttttg gaagtaagta aacaatttgt taaacactta aatataaact aattatgaat
      181 gactttttag acagtgacga ggaaagagac agaagacctg tttttttaac ggtagttgga
      241 atactcagtt tggtaaacat cagctggtcg ttgattcaaa atataacttc gtttttaagc
      301 ggaccattaa cggaagaagg aatggaagcc taccgtttgg aaatggcaaa aagcttaagt
      361 acggtgcaag atacaggtgt ggtatgggca gaagacttga ttcaaagtgc tatgaatatg
      421 gtagaatcta taaatgtcaa tcatacaatg aatttattgt cgagcaccgt tattttaatc
      481 attggacttg cagctgttat catgatgttg aaaggtagaa aaataggatt tcacggctac
      541 atcatctaca gttttttagc ttctgtgcaa atttatttgt ttgtaacgcc atcgttgttg
      601 ccgaatatta tggtaatatt ctcattattg gctagtggcc tgtttattct gttttatggc
      661 ttgaacttga aatggatgaa ataatttacg tttttatata aacgaaaaag aggttgaatt
      721 ttgcagttca acctcttttt tttggtaaga attgtatttt tatttctgta tggttataaa
      781 accatggcat tttgatttgt agggctcttc tttgcaattg aaatcatccg aaacgatttt
      841 gtagaaatat accccatccg aaagttctgt tcctttttta tcttttccat agaaaatagg
      901 attatcctta gaagcttcaa agattggatg tccccaacgg ttaacaaaag ttactttgta
      961 attaatgcat tctaaatcca ttaaaggaaa gacaacgaaa tcgtttactc catccccatt
     1021 aggtgtgatg atgtttggaa tgttgaagtt ttctgctgtc acacctgtag gcacgttaac
     1081 aattgcactt gtagttgctt gacaaccgtt tgcatctgca gcagtcaaag cataagtatg
     1141 atctccagct gtttgggcgg tgtacaccaa tggatttcca gttccaatag ctgttgttcc
     1201 atccaaccaa ttgtatgaag cataatttgc tggtgttgcc gttaatgtta ccgtttgaaa
     1261 acaatctaca cttgcttgaa attgaacagt caattcactt ccgatggtaa agttgtgtgt
     1321 gtagctacta tgacaacctt gc
//
LOCUS       DAOQVQ010000583         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_141707_length_1333_cov_2.894366, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000583 DAOQVQ010000000
VERSION     DAOQVQ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141707_length_1333_cov_2.894366"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..527
                     /locus_tag="PKN22_12155"
     CDS             123..527
                     /locus_tag="PKN22_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS43502.1"
                     /translation="MKTILGCLIVFFTHTAYSQTYAETIDFNKLSLQFEHASADLKPD
                     YFALVYRLADTLKRNPNLHLTIRGHVCCVNKNGLAKKRAKVVKNFLILFGVDKKQMTT
                     LGMKNTMPVVFPEKTREDELANMRVDFVLKEH"
     gene            540..>1333
                     /locus_tag="PKN22_12160"
     CDS             540..>1333
                     /locus_tag="PKN22_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNS43503.1"
                     /translation="MKHLLILLTLCSSISLAQSTINGSFIHGGINRTYSYYVPASYSA
                     GTPVPLVLNLHGYTSNGTQQSFYTNFQPIADTANFIIVHPNGTNDPVGNQPFWNFNIW
                     GATVDDLGFLQALIDTISAHYSINQDRIYSVGMSNGGFMSYYLACNTNRFAAVGSVTG
                     SMSFDMYNNCNSMLPVPIIDIHGTADPTVPYNGNATSKPIEDVVDFWVARNNCSTTPT
                     VTTVPDINTTDGATAEHYVYAGGVENHSVEFFKITNGGHTWPGATIP"
BASE COUNT          383 a          300 c          239 g          411 t
ORIGIN      
        1 ctcctttttc cataacgata aaaaattgtg tatcgctatt tcaggactag acatttagtt
       61 tttcataaaa ggcttgcatt tttggtgcag gcctttgttt ttttttgtat tattgtaatg
      121 ctatgaaaac aattttaggc tgcttaatcg tattttttac ccacactgca tattcgcaaa
      181 cgtatgcaga aacgattgat ttcaataaac tctccctcca atttgagcac gcttcagctg
      241 atttaaaacc cgattatttt gctttggttt accgcctagc cgacacctta aaacgcaatc
      301 ccaacctaca ccttaccatt cgcggacatg tgtgttgtgt taacaaaaac ggattggcta
      361 aaaaaagagc taaagtggtg aaaaacttct taatcctctt tggagtggat aaaaaacaaa
      421 tgaccaccct tggtatgaaa aacacaatgc ctgttgtatt tcctgaaaaa actagagaag
      481 acgaattggc caacatgcgc gttgattttg tcttaaaaga acactaaaaa acaattgcta
      541 tgaaacattt attgatacta ctaactcttt gcagttctat atcgctggca caatccacca
      601 tcaacggttc gtttatccac ggaggcatta atcgcacgta ttcttattac gttccggcaa
      661 gttattcggc aggcacacct gttccactgg ttttaaattt gcacggatac acctccaacg
      721 gcacacaaca atccttctac accaattttc aacccattgc tgatacggct aattttataa
      781 ttgttcatcc caacggaacc aatgatcctg tgggcaatca accgttttgg aatttcaata
      841 tttggggagc aacggtggat gatcttggat ttttacaagc tctaattgac actatttctg
      901 cacactattc catcaatcaa gaccgtattt attcggttgg aatgtcaaac ggtggtttca
      961 tgagctatta tttggcgtgc aacaccaatc gttttgcggc tgttggctct gttactggtt
     1021 ccatgtcgtt tgatatgtac aacaattgca actccatgct tcctgttcca attatcgaca
     1081 tccatggcac tgccgaccca actgttccat acaacggcaa cgccacttcc aaacctattg
     1141 aagatgtggt tgatttttgg gttgctagaa acaattgctc caccactcca actgttacaa
     1201 cggttccaga catcaacaca accgatggtg caactgccga gcattacgta tatgcgggtg
     1261 gagttgaaaa tcattcggta gaatttttta aaattaccaa cggtgggcac acatggcctg
     1321 gagcaactat tcc
//
LOCUS       DAOQVQ010000584         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_142369_length_1329_cov_5.456829, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000584 DAOQVQ010000000
VERSION     DAOQVQ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142369_length_1329_cov_5.456829"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1329)
                     /locus_tag="PKN22_12165"
     CDS             complement(<1..>1329)
                     /locus_tag="PKN22_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS43504.1"
                     /translation="NVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSDYRLFYDTV
                     KGGDYRSREANVYRLAEVSASIIDQCVAQGVPFARDYGGLLDNRSFGGVQVSRTFYAK
                     GQTGQQLLLGAYSAMNRQIAKGKITMYNRHEMLELVVVNGKARGIIARNLVTGAIERH
                     SAHAVVLGTGGYGNVFFLSTNAMGSNASAAWRAHKKGAYFANPCYTQIHPTCIPRSGD
                     YQSKLTLMSESLRNDGRIWVPKNIEDAVAIREGRLKPTQIKEEDRDYYLERRYPAFGN
                     LVPRDVASRAAKERCDAGYGVNKTGEAVYLDFSSAIMRYGKEKANLMHEHNASEDRIR
                     ELGEAVVESKYGNLFQMYEKIVDDNPYKTPMMIYPAVHYTMGGLWVDYNLMTTIDGCY
                     CIGEANFSDHGANRLGASALMQGLADGYFVLPYTIGDQLQHEIRTGKIST"
BASE COUNT          357 a          307 c          244 g          421 t
ORIGIN      
        1 tctgtagaaa ttttacccgt gcgaatttcg tgttgcaatt gatcaccaat tgtataaggc
       61 aatacgaagt atccgtcagc taacccttgc atcaatgcag atgctcctaa gcggttagct
      121 ccgtgatcag agaagttcgc ctctccaata cagtaacaac cgtcaattgt tgtcatcaag
      181 ttgtaatcaa cccaaagtcc acccattgta tagtgtaccg caggataaat catcattggt
      241 gttttgtatg ggttatcatc aacgattttc tcatacattt ggaataagtt tccgtattta
      301 gattcaacaa ctgcttcacc caattctctg attctatctt cagaagcatt gtgttcatgc
      361 attaagttag ctttttcctt accatagcgc atgatagctg agctaaaatc caagtaaacc
      421 gcttcacctg ttttgttaac tccgtaacca gcgtcacatc tttctttcgc agcgcgcgat
      481 gcaacgtcac gtggaaccaa gttaccaaat gcagggtatc ttctttccaa atagtaatct
      541 ctatcttctt ctttaatttg tgttggtttc aaacgtcctt cgcgaattgc aactgcatct
      601 tcgatgtttt ttggcaccca aatacgtcca tcgtttctta acgattctga catcaacgtt
      661 aatttagatt ggtaatcacc agaacgtgga atacatgttg ggtgaatttg cgtataacaa
      721 ggatttgcaa aataagctcc ttttttgtgt gctctccatg ctgctgatgc gtttgatccc
      781 atagcattgg ttgataagaa gaatacgttt ccgtaacctc ctgttccaag cactaccgca
      841 tgtgcagagt ggcgttcgat tgctccagta accaagttgc gtgcaataat tcctcttgct
      901 ttaccattta caacaaccaa ttccaacatt tcgtggcggt tatacatggt gattttacct
      961 tttgcaattt gacggttcat tgctgaataa gcacccaaca acaattgttg tcctgtttgt
     1021 cctttggcat aaaaagtacg tgaaacttgc accccaccaa aagaacggtt gtctaacaag
     1081 cctccgtaat cgcgtgcaaa aggaactcct tgcgccacac attggtcgat aatgcttgca
     1141 gacacctcag ccaaacggta aacatttgct tctcttgaac ggtagtcacc accttttacc
     1201 gtgtcgtaaa acaaacggta atcagagtca ccatcatttt gataattttt tgctgcgttg
     1261 ataccacctt gagctgcaat tgagtgtgct ctacgtggag aatcttggaa acaaaatgct
     1321 tttacattg
//
LOCUS       DAOQVQ010000585         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_142471_length_1329_cov_2.264521, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000585 DAOQVQ010000000
VERSION     DAOQVQ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142471_length_1329_cov_2.264521"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKN22_12170"
     CDS             complement(<1..1080)
                     /locus_tag="PKN22_12170"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNS43505.1"
                     /translation="MSTQNKEQAHDSMFEAVLARLDVAAKLMNLSDDVTTILRSPQKQ
                     VQVSLPIVMDNGKVQVFDGYRVVHSTILGPSKGGIRFAMDVNQDEVMALSAWMSFKCA
                     VANLPYGGAKGGIKCDPRSMSVGELERLSRAYAKSMKDVFGVNKDIPAPDMGTGGREM
                     AWMLDEFNKVTGEDSPGVITGKPIVIGGSLGREAATGRGVMVNTLAALKKMNIDPTTV
                     SCAVQGFGNVGSNAAILLAEKGLKIVAIGDHTATFYNENGIDINAAVEYAAKNDRVLK
                     GFTGGTEIAPNDLLTCKCDVLVPAALQNVITEDIAKNIQAKLIVEGANGPTTPEADPI
                     LNERNIIVVPDILANSGGVTVSYFEW"
BASE COUNT          399 a          281 c          183 g          466 t
ORIGIN      
        1 ccattcaaag taagaaacag ttacaccacc actgtttgcc aaaatatctg gaaccacaat
       61 aatgtttctt tcatttaaaa ttggatccgc ttctggtgtt gttggtccgt ttgcaccttc
      121 aacaatcaat tttgcttgaa tgtttttagc aatatcttct gtaattacgt tttgcaatgc
      181 agcaggaacc aatacgtcac atttgcatgt caacaagtca tttggagcaa tttcagttcc
      241 acctgtaaat cctttcaaca ctcggtcgtt tttagcagca tattcaacag ctgcattgat
      301 atcaatacca ttttcgttgt agaaagttgc tgtgtggtca ccaattgcaa caattttcaa
      361 tcctttttct gccaataaaa tagctgcgtt agaaccaaca tttccaaatc cttgaactgc
      421 acaagaaact gtagttggat caatattcat tttcttcaaa gcagccaatg tgttcaccat
      481 tacaccacga ccagttgcag cttcacgtcc taaagaacct ccaattacaa ttggttttcc
      541 tgtgataaca cctggcgaat cttcacctgt tactttattg aattcatcca acatccaagc
      601 catttcgcgg ccacctgtac ccatatctgg agctggaatg tctttattta ctccaaaaac
      661 atctttcatg ctttttgcat acgcacgcga taatctttcc aattcaccaa cactcattga
      721 acgagggtca catttaattc caccttttgc accaccataa ggtaagttag caacggcaca
      781 tttaaaactc atccatgcac tcaatgccat cacttcatct tggtttacat ccatcgcaaa
      841 acgaattccg cctttacttg gacctaatat tgtagagtga acaacacgat aaccatcaaa
      901 tacttgaact ttgccattgt ccataacaat tggcaaacta acttgtactt gtttttgtgg
      961 acttcttaaa atagtagtta cgtcatcaga taaattcatc aattttgctg ctacatctaa
     1021 acgtgctaaa actgcctcaa acattgaatc gtgtgcttgt tctttatttt gtgttgacat
     1081 gatttattta attataaatt tacaatcgca aatgtagcaa tatttagaaa tatttcaatt
     1141 ttttcattaa aattttaact aatataacaa cttgattatc agttttataa ataatttttg
     1201 cctcaaatac acttgaacaa gttaaaatta tcttattttt ttaggtttat gaggatattg
     1261 aaattgatac tatttaatgg tttatttatt cttttcgcaa aaggttggct ctctattttt
     1321 cctggattt
//
LOCUS       DAOQVQ010000586         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_143854_length_1321_cov_3.417062, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000586 DAOQVQ010000000
VERSION     DAOQVQ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143854_length_1321_cov_3.417062"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /gene="pyrH"
                     /locus_tag="PKN22_12175"
     CDS             <1..269
                     /gene="pyrH"
                     /locus_tag="PKN22_12175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687233.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNS43506.1"
                     /translation="IEIEADVILKGTRVDGIYTADPEKDPTATKYDAITFDEAYSKGL
                     KVMDMTAFTLCMENDLPIVVFDMDTVGNLKKVIAGDKIGTIVHN"
     gene            419..>1321
                     /locus_tag="PKN22_12180"
     CDS             419..>1321
                     /locus_tag="PKN22_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S9"
                     /protein_id="HNS43507.1"
                     /translation="MIKHILLILTLGSFMAHAQEVLTPEKMMALKRVGGGTVSPDGKF
                     LLFSQTSIDVEQNKGNTDLFVMTIADKSIKQITNTPVSEASAQWVDGNKIYFLAPEKD
                     QAVIWRVDVDGANKTQISENVEGIEDFKITKDASKIIVLKSVEIRKNINQTYTDLPKA
                     NARVENDLMYRHWASWDDYTVKHIFYYPLNNGKISGEGKDLLKDEQFNAVIPPFGDLG
                     GVTFTPNNQSVIYSSKKKSGKEFALSTNSELYQVELETGKTICLTNTNEFKGYESHPA
                     FSVKGDLAWLAMKRDGFEADKNDII"
BASE COUNT          447 a          207 c          251 g          416 t
ORIGIN      
        1 caattgaaat tgaagcagac gttattttga aaggaacacg cgtggatgga atttacacag
       61 ccgatccaga aaaagatcca actgctacaa aatacgatgc cattactttt gatgaagcct
      121 attcaaaagg gttgaaagta atggatatga ctgcatttac tttgtgtatg gaaaacgatt
      181 tacctatcgt tgtgtttgat atggataccg ttggaaacct caaaaaagta attgcaggcg
      241 ataaaattgg tacaatcgta cataattaat ctctattttt tttcatttca tgtttgctga
      301 tttattttgg cgagctattt gtttgtgcta ttgcgtgtaa ttcaaagtgc aaggtttttc
      361 tttgaatttt caagacttaa ttttttttag tacattcacg cagtaaattc atctaaaaat
      421 gataaaacac attcttctaa ttttaacgtt aggtagcttt atggctcatg cacaagaggt
      481 tttaactccc gaaaaaatga tggctttgaa acgtgtcggt ggtggcactg tctcgccaga
      541 tggtaagttt ttattatttt ctcaaacgtc cattgatgtt gaacaaaaca aaggaaatac
      601 cgatttattt gtaatgacaa ttgccgataa atcaattaaa caaattacca atacaccagt
      661 ttcagaagcg agtgcacaat gggttgatgg caataaaatt tattttttag cacccgaaaa
      721 agatcaagct gtaatttggc gagttgatgt ggatggagct aataaaaccc aaatttcgga
      781 aaacgtggaa ggcatcgaag attttaaaat caccaaagat gcttccaaaa ttattgtctt
      841 aaaatcggtt gaaattcgca aaaatattaa tcaaacctat acagatttac caaaagccaa
      901 tgcacgtgta gaaaacgatt tgatgtatag acattgggct agttgggatg attataccgt
      961 aaaacatatt ttttattacc ccctaaacaa tggaaaaatt tcgggtgagg ggaaagattt
     1021 actgaaagat gaacagttca atgctgttat tcctcctttt ggtgatttgg gtggcgtaac
     1081 atttactccc aacaaccaat ctgttattta cagctctaag aaaaaatcag gaaaagaatt
     1141 tgctctttca accaacagtg agttgtatca agtagaattg gaaacaggaa aaacaatttg
     1201 tttgactaac accaatgaat ttaagggata cgaatcacat cctgcttttt ctgttaaagg
     1261 agatttggct tggttggcca tgaaacgtga tggttttgaa gccgataaaa acgacatcat
     1321 t
//
LOCUS       DAOQVQ010000587         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_145160_length_1314_cov_2.891978, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000587 DAOQVQ010000000
VERSION     DAOQVQ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145160_length_1314_cov_2.891978"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>1314)
                     /locus_tag="PKN22_12185"
     CDS             complement(11..>1314)
                     /locus_tag="PKN22_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS43508.1"
                     /translation="LSKSKSTILMVTHDRYFLEVVCDTILELDNKTLYRYQGNFSYFL
                     EKKAEREEQLEATIDKARNQFRKELEWMRRQPKARGTKQKARIEAFHDTKKVAHQRID
                     KDEMDIPVKMERLGSKILELHHIGKSFGEKKILNDFSYNFKRQERVGIVGNNGTGKST
                     FLNMILEKEPVDKGKIVVGETVVFGYYNQEQLQVEDDKKVIDVIRDVAEYIPLEKGRQ
                     LSAAQFLEKFLFPRDMHYNFVHKLSGGEKRRLKLMTVLMKNPNFLILDEPTNDLDIFV
                     MGVLEEYLRSFEGCLIVVSHDRYFMDKMVDHLFVFEGNGEIKDIIGNYSEYRVQQKAT
                     ISQQRAEEKKEEIQQQQPEITTSSEPKEKRKLSYKEQKEYETLETEIEQLEQRKEELA
                     AILSSAESSNTQLMEAGEELTKVVASIDEKSNRWLELAEFV"
BASE COUNT          345 a          274 c          212 g          483 t
ORIGIN      
        1 acaatcacgc tcacacaaac tccgctaact ccagccaacg attggatttc tcgtcaatag
       61 aagcaacaac tttggttaac tcctcacctg cttccatgag ttgagtattg gaactttcgg
      121 ctgatgataa tattgcagct agttcttctt tgcgttgctc taattgctct atttcagttt
      181 ctagtgtttc gtattctttt tgttctttat aagacaattt ccttttttct tttggctctg
      241 atgaagttgt tatttcgggt tgctgttgtt gaatttcttc tttcttttcc tcggcacgtt
      301 gctgcgaaat agttgctttt tgttgaacgc ggtattctga atagttgcca ataatgtctt
      361 taatttctcc gtttccttca aaaacaaaga gatgatcaac cattttatcc ataaaatatc
      421 ggtcgtgtga aaccacaatt aaacacccct caaatgagcg caaatattct tctaacacgc
      481 ccatcacaaa aatatctaaa tcgttggtag gttcatcgag aataaggaaa ttagggtttt
      541 tcatcaatac cgtcatcaac ttcaaacggc gtttttctcc cccacttaat ttgtgcacaa
      601 aattgtagtg catatcgcgt ggaaacaaga acttttctaa aaactgtgca gcagaaagtt
      661 gtcggccttt ttccagtgga atgtattcgg caacatcgcg aatgacgtct atcacttttt
      721 tgtcgtcttc cacctgtaat tgttcttggt tataatatcc aaatacaaca gtttctccca
      781 ccacaatttt tcctttatca actggttctt tttctaaaat catatttaga aaagtggatt
      841 tcccggttcc gttgtttcca acaatcccaa ctctttcttg acgtttaaaa ttgtaggaaa
      901 aatcattgag tattttcttt tcgccaaaag attttccaat atggtgtaat tccaatattt
      961 tagaacccaa acgttccatt ttaactggaa tatccatctc atctttgtcg atgcgttggt
     1021 gtgccacttt tttggtgtcg tgaaaagctt ctatgcgtgc tttttgtttg gttccacgag
     1081 cttttggctg tctacgcatc cattccaact ctttacgaaa ctggttgcgt gccttatcaa
     1141 tagtagcttc taattgctct tctcgctctg cttttttttc taaaaaatat gaaaaattac
     1201 cttgatagcg gtacaatgtt ttgttgtcca attctaaaat agtatcgcac actacttcca
     1261 aaaaatagcg gtcgtgcgta accattaaaa ttgtagattt tgattttgat aaat
//
LOCUS       DAOQVQ010000588         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_145318_length_1313_cov_3.556439, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000588 DAOQVQ010000000
VERSION     DAOQVQ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145318_length_1313_cov_3.556439"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKN22_12190"
     CDS             complement(<1..605)
                     /locus_tag="PKN22_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS43509.1"
                     /translation="MKILIIGNVWPEPNSSAAGKRMTQLIDLFSNFGEIHFACANQLT
                     GNEIDFQKFGIQPHFIELNNDTVDQFFVELNPAIVVFDRFLTEEQYGWRIIEQCPNAL
                     RILDTEDLHFLRRTRHQFVKKNNAVANKLEALDFQNNDTLRELASIYRCDVSLIISPF
                     EMELLTTEMQVPEELLYYLPIFSSAISETPTFEAKKDCLFIG"
     gene            685..1074
                     /locus_tag="PKN22_12195"
     CDS             685..1074
                     /locus_tag="PKN22_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43510.1"
                     /translation="MKHLLLLLVAFSLFSCTKKQDKACTALFAMITIQVKDTLNNPVL
                     LDSTSIFVPSTNSYLSILTDDFYKSNGEYVVFSDEFIHLTSEKKSLEIIFKGFKNNSL
                     KVNESIKVSQDGCHVTKVSGNSSVIVQ"
     gene            1157..>1313
                     /locus_tag="PKN22_12200"
     CDS             1157..>1313
                     /locus_tag="PKN22_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS43511.1"
                     /translation="MKQATIVGAGLVGSLWATYLAKAGYQVTIFESRSDLRNLQLTAG
                     KSINLALS"
BASE COUNT          456 a          212 c          219 g          426 t
ORIGIN      
        1 ccaataaaca agcaatcttt tttagcttca aaagttggtg tttcagaaat tgcagatgag
       61 aaaataggca agtaatacaa taattcttct ggcacttgca tttcggttgt gagcaactcc
      121 atttcgaaag gtgaaattat gagagaaaca tcacaacgat aaatagatgc caattcacgc
      181 aaggtatcgt tattttgaaa atctaatgct tctaatttat tagcaactgc attgtttttt
      241 ttaacaaatt gatggcgtgt gcggcgtaaa aaatgtaaat cttcggtatc taaaatccgc
      301 aaggcattcg gacattgttc aatgatgcgc caaccatatt gttcttcggt tagaaaacgg
      361 tcaaacacaa caattgctgg atttaattct acaaaaaatt ggtcaacagt gtcgttattc
      421 agttctataa aatgcggttg gattccaaac ttttgaaaat ctatctcatt ccccgtcaac
      481 tgattggcac aagcaaaatg gatttctcca aaattggaaa aaaggtcaat taattgtgtc
      541 attcgcttac cagcagccga agaattgggt tctggccaaa catttccgat gattaaaatt
      601 ttcattcaat gaaattatgc aacaaaaata gagatagata tcagaaaaaa tgattaattt
      661 gtagaatatt tgaatttcgg tattatgaaa catttactac ttctattggt tgcttttagt
      721 ttgttttctt gcacgaaaaa acaagataaa gcttgcacag cattatttgc aatgattact
      781 atacaagtta aggatacact taacaatcca gtgctacttg attcgaccag tatttttgta
      841 ccttcaacta atagttatct atccattcta accgatgatt tttacaaatc gaatggtgaa
      901 tatgttgttt tttctgacga gtttattcat ttaacatccg agaaaaaatc acttgaaatt
      961 atttttaaag gatttaaaaa caatagttta aaggttaatg aatcaataaa agtttcacaa
     1021 gacgggtgtc atgtcaccaa agtttctgga aactcttcgg ttattgttca gtagtgttaa
     1081 aaacaaacct cgcatagccg aatactaaaa tttcattatt tttagcctca ttatcaaacc
     1141 atacacagac taaaaaatga aacaagcaac aattgtggga gctggattag tgggttcttt
     1201 atgggcaact tatcttgcaa aagcgggtta tcaagttact atttttgaaa gcagaagcga
     1261 tttaagaaat ttgcaattaa ctgctggaaa atcaatcaat ttagcgcttt caa
//
LOCUS       DAOQVQ010000589         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_145869_length_1310_cov_3.058964, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000589 DAOQVQ010000000
VERSION     DAOQVQ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145869_length_1310_cov_3.058964"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKN22_12205"
     CDS             complement(<1..199)
                     /locus_tag="PKN22_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43512.1"
                     /translation="MILSATGVFRTILIIIGIIVVLRFIGQLLNAKRNMETERKLNQN
                     AREFEEERLRTFKNYGKTRIIK"
     gene            complement(393..860)
                     /locus_tag="PKN22_12210"
     CDS             complement(393..860)
                     /locus_tag="PKN22_12210"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNS43513.1"
                     /translation="MTLYQFLVFIHVMSAILGLGPGFVMIYIVTKAQTMTELRHAYMI
                     RNRVHVFVMVGGTLLILSGLAMGLINNYLFHAVWYLASLSLFLIALAFGPIVLSPCSK
                     PIKELIKNHQGDDIPAEYYPLAKKLFFYERIENVMFLIIIALMIFKPFGVIQF"
     gene            complement(962..>1310)
                     /locus_tag="PKN22_12215"
     CDS             complement(962..>1310)
                     /locus_tag="PKN22_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNS43514.1"
                     /translation="EMLIASAGNPIEGFNQLLIPEDKLPKMLAFLDSEDPMPREFKAP
                     FAPEVYELKNEELELAIDELVEAWLNYEEYYETHPTATQMHPYYGMCDKTKWDRIHQK
                     HFTHHFQQFGLVD"
BASE COUNT          444 a          228 c          231 g          407 t
ORIGIN      
        1 ccttgatgat gcgtgttttt ccgtagtttt tgaaagttcg cagacgttct tcttcaaatt
       61 cacgtgcatt ttggttaagt tttcgttcgg tttccatgtt gcgtttagca tttaaaagtt
      121 gtccaataaa tcgtaaaacc acaattattc cgataattat taaaatggta cgaaaaacgc
      181 ctgttgcact aagaatcata acaattggta ttttttacaa atataatcaa aatagacgga
      241 cgaacaaata aaacaaagtg accaatatca ctgaatagtt gaatgcattt tcagacattt
      301 gcataactca ttaggtaaaa gttaaatagt tttaatgtta atcgtgattt attgttaagt
      361 tagaagtcgt gcagcaatgt gcgactttta aattaaaact gaataacgcc aaatggttta
      421 aaaatcatta gagctatgat aattaaaaac ataacattct caatgcgttc gtaaaagaac
      481 agtttttttg ccaaaggata atattcggct ggaatatcgt cgccttgatg gtttttgatt
      541 aattctttaa ttggtttaga acaaggtgat aacacgatgg gaccaaaagc caaggcgatt
      601 aaaaacaagg ataaacttgc caagtaccaa acggcatgaa acaaatagtt gttaatgagc
      661 cccattgcta aacccgacaa aattaacaag gttcctccca ccatcacaaa tacatgcacc
      721 cggttgcgaa tcatataggc gtggcgcaat tcggtcattg tttgtgcttt tgtaacaatg
      781 taaatcatca caaagccagg tcctaagcct aggattgccg acattacgtg aatgaatact
      841 aaaaattggt acaaagtcat tctgttgctt tatttttcta ctactagaac tatcggtgcc
      901 aaatttaaga ataattctaa ataaggacca aaaaagcaat cgaaaagaat aaaaaaagga
      961 attaatctac caatccaaac tgttggaaat ggtgtgtaaa gtgtttttgg tgaattctat
     1021 cccattttgt cttatcacac attccgtaat agggatgcat ttgagttgct gtgggatgag
     1081 tttcataata ttcttcgtag ttcaaccacg cctccaccaa ttcatcaatg gctaattcta
     1141 actcttcgtt tttaagttca tacacttctg gagcaaaagg agctttaaat tcacgaggca
     1201 taggatcttc ggaatctaaa aacgccagca ttttaggcaa tttatcttcg ggaataagca
     1261 attgattaaa accttcaata gggtttccag ccgatgcaat gagcatttcg
//
LOCUS       DAOQVQ010000590         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_148606_length_1295_cov_4.229839, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000590 DAOQVQ010000000
VERSION     DAOQVQ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148606_length_1295_cov_4.229839"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PKN22_12220"
     CDS             <1..1153
                     /locus_tag="PKN22_12220"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNS43515.1"
                     /translation="ALIRVDFNVPLNKETFEVTDDTRIRAAAPTIQHILKNGGSVVLM
                     SHLGRPDGVQEKYSLKHIVSQVSKVLGVDVKFVDDCINEKALEASKNLKPGEVLLLEN
                     VRFYKQETAGTESFAEVLAKHGDVYVNDAFGTAHRAHASTTVVAKFFPNDKMFGYLLE
                     GEIKAVDRVLNSNEKPLTAIVGGAKVSSKITIIDRLLNKVDNLIVGGGMAYTFIKAKG
                     GNVGNSLVEDDYLVIAKNILLKAEAKRINLYLPVDTIVADKFDNNAEQKLVNINDIPQ
                     GWMGLDVGPESSKEIRDIIVNSKLILWNGPMGVFEMENFQKGTIEVANAVVEATEKGA
                     FSLIGGGDSVAAINLFGLADKVSYVSTGGGAMLEYLEGIELPGIKAINE"
BASE COUNT          428 a          182 c          296 g          389 t
ORIGIN      
        1 cgccttgatt cgtgttgatt ttaacgttcc attgaacaaa gaaacatttg aagttacaga
       61 tgatacacgc attcgcgcag ccgctccaac aattcaacat atactaaaaa atggcggaag
      121 tgtggtttta atgtcgcacc ttggacgacc agatggtgtg caagaaaaat attcgttgaa
      181 acacatcgtt agtcaagttt ctaaagtttt gggtgtggat gtaaaatttg tggatgattg
      241 tatcaatgaa aaagcattag aagcaagtaa aaacctgaag ccaggtgagg ttttattgtt
      301 agaaaacgtt cgtttttata aacaagaaac tgctggtaca gagtcgtttg ctgaggtact
      361 ggcaaaacat ggagatgtgt atgtgaatga tgcttttggt acagctcacc gtgcgcatgc
      421 atctacaacc gttgtggcaa aattcttccc aaatgataaa atgtttggtt atttgttgga
      481 aggtgaaatt aaagcagttg atagagtatt aaatagcaat gaaaagccac taacagcaat
      541 tgttggtggt gcaaaagtat cttctaaaat tacaattatc gatcgtttgt tgaacaaagt
      601 agataaccta attgttggtg gaggtatggc ctatacattc atcaaagcta aaggtggaaa
      661 cgttggaaat tctttggtag aagacgatta tttggtaatt gctaaaaata ttttgttaaa
      721 agcagaagca aaacgcatca atttatattt gcctgttgat acaattgttg ccgataaatt
      781 tgacaacaat gcagaacaaa aattggtgaa catcaacgac attccgcaag gatggatggg
      841 attggatgta gggccagaat cgtctaaaga aatcagagac attattgtta attctaaatt
      901 gattctttgg aacggaccaa tgggtgtatt tgaaatggaa aacttccaaa aaggaactat
      961 tgaggtggca aatgcggtgg ttgaagctac tgaaaaaggt gcattttcat taattggagg
     1021 aggagattct gttgcagcca tcaatttatt tggtttagcc gataaagttt cgtatgtttc
     1081 tactggtgga ggtgccatgt tggaatattt agagggtatc gaattgccgg gaattaaagc
     1141 aattaacgaa taatgatgaa attaaaattt agtctattgt ttttgttcgc agcactagtt
     1201 gttggagctc aagcacaagt aaaaaattta aaattgacca actgcgtggt ggttggtcaa
     1261 tttgataaac ccgaggatcg ctttacaatc gaaag
//
LOCUS       DAOQVQ010000591         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_148830_length_1294_cov_3.167877, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000591 DAOQVQ010000000
VERSION     DAOQVQ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148830_length_1294_cov_3.167877"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKN22_12225"
     CDS             complement(<1..508)
                     /locus_tag="PKN22_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS43516.1"
                     /translation="MNYKNFSEEDIVKRVINGEKELYEIIVRRFNPFLYKVGRSYNFN
                     HDDTMDLMQDTFVDAYKNLANFQGRSSFKTWIIRIMLNNCYHKKSKFSFKNELALEIH
                     ENATPMYSHKSNETEKIVHNNELNHIIENALGQLPEDYRMVFSLREINGLSVNETAEL
                     MGKRTQRKS"
     gene            complement(603..980)
                     /locus_tag="PKN22_12230"
     CDS             complement(603..980)
                     /locus_tag="PKN22_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43517.1"
                     /translation="MESKNQVLNQTYNLLKYVFVIVPIVAGLDKFTNILTDWTQYVNP
                     SIEGMLPFSAVTFMMVVGVIEIVAGILVFIKPQIGGLVVAAWLVGIALTLIAGWMYVD
                     VAVRDLVMAIGAFSMARLSQIIK"
     gene            complement(1098..>1294)
                     /locus_tag="PKN22_12235"
     CDS             complement(1098..>1294)
                     /locus_tag="PKN22_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNS43518.1"
                     /translation="TALVFSSYKQNTHWLALVGILLTILLEYIFRHFHLFDAFGGVLT
                     FPFVLATWVTLFINKNIFNK"
BASE COUNT          437 a          215 c          190 g          452 t
ORIGIN      
        1 tacttttacg ttgggttcgc tttcccatta attctgctgt ttcgtttacg cttaatccgt
       61 taatctcacg taaagaaaac accatgcgat agtcttctgg caattgtccc aaagcatttt
      121 caatgatgtg atttagctca ttgttgtgaa ctattttttc tgtttcattt gacttgtgac
      181 tgtacatagg tgttgcattt tcatgaattt caagtgccaa ttcattttta aaactaaatt
      241 ttgatttttt gtggtaacaa ttattcaaca ttatccgaat tatccatgtt ttaaatgaag
      301 aacgtccttg aaaatttgct aagtttttat acgcatctac aaaggtatct tgcattaagt
      361 ccatggtatc gtcgtgatta aaattgtagg atcgtccaac tttatataaa aacggattaa
      421 agcgtctgac gataatttca tacaactctt tttcaccgtt aattacacgt ttaacaatgt
      481 cttcttcaga aaaattttta taattcattg gtttataaat aattgttttc aacctctaag
      541 ttaatgctat cctctaaaat aacaaagagg atagcatatt tatattagaa gggttagatt
      601 ttctatttta ttatttgtga aagtcgtgcc attgagaaag caccaatcgc catgactaaa
      661 tcgcgtacag ctacatcaac atacatccaa cctgcaatta aagtaagtgc aatacctacc
      721 aaccaagccg ctacaactaa tccgccaatt tgtggtttga tgaataccaa tatacccgct
      781 acgatttcaa tcactccaac taccatcata aatgttacag ctgagaaagg caacattcct
      841 tcaattgatg ggtttacata ttgcgtccaa tctgttaaga tgttggtaaa tttatccaat
      901 ccagcaacaa ttggtacgat aacaaaaaca tacttcaaca aattgtatgt ttgatttaaa
      961 acttgatttt ttgattccat ttttttaaaa ttttaattat taattggttt tatacccatt
     1021 aggggaaatg aaatcaaaaa aggttacaag aaaaatgaat tattttttaa taaaataagc
     1081 aactaattga ttttgtgtta tttattgaaa atatttttat taataaaaag tgtaacccat
     1141 gttgccaaca caaaaggaaa tgttagtacc ccaccaaaag catcaaaaag atggaaatgt
     1201 ctaaagatgt attctaagag aatggttaga aggattccaa ctaacgccaa ccaatgggta
     1261 ttttgtttgt aggacgaaaa aactaatgct gtta
//
LOCUS       DAOQVQ010000592         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_151115_length_1282_cov_3.727791, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000592 DAOQVQ010000000
VERSION     DAOQVQ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151115_length_1282_cov_3.727791"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1282
                     /locus_tag="PKN22_12240"
     CDS             <1..>1282
                     /locus_tag="PKN22_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNS43519.1"
                     /translation="ITEESAQDLPSVQVGDPFMEKLLLEATMELANTDAIVGMQDMGA
                     AGITCSTCEMSAGGGVGMEIHLDKVPTRQDNMLPYEILLSESQERMLVVVHKGKEQVV
                     KDIFDKWDLHAEEIGVVTDTGRIRYYMHGELVGDVPAESLVLGGGAPQYEREYKEPAY
                     YQEYKKFNIDSVKEPENLKEVADFLLQHPNICSKRWVYEQYDSMVGTKNMTTNRPSDA
                     GVVNIKGTDKALAMVVDCNSRYVKADPEVGTMIAVSESARNVVCSGGVPSAITNCLNF
                     GNPYNPESYWQFVGAIKGMAAACTKFQTPVTGGNVSFYNQSVINGVEEPVFPTPTIGM
                     LGVLEDKNKMMTLDFKKKGDLIFLLGESVNDIASSEYLYSYHGIKASPAPYFDLEKEF
                     AVQEVVKDLIQSELINAAHDVSDGGLFITLAEMSF"
BASE COUNT          405 a          207 c          304 g          366 t
ORIGIN      
        1 attacagaag aatcggcaca agatttacct tctgttcaag ttggtgaccc attcatggag
       61 aaattgttgt tggaagcaac tatggaattg gctaataccg atgcaattgt ggggatgcaa
      121 gacatgggag cagcaggtat tacttgttca acatgtgaaa tgagtgctgg aggtggtgtt
      181 ggtatggaaa tccatttgga taaagtgcct acgcgccaag ataacatgtt gccatacgaa
      241 atcttgttgt cagaatcaca agagcgtatg ttggtggttg tgcacaaagg aaaagaacaa
      301 gtagtaaaag atatttttga taagtgggat ttgcacgccg aagaaattgg ggtggttaca
      361 gatactggta gaattcgtta ctacatgcac ggtgaattgg taggagatgt tcctgcagaa
      421 tcattggtgt tgggtggtgg agcgccgcaa tacgagcgtg agtataaaga acctgcttat
      481 taccaagaat acaaaaaatt caacattgat tcggtaaaag agccagaaaa cttaaaagaa
      541 gttgccgatt tcttgttgca acatccaaac atttgttcaa aacgttgggt gtatgaacaa
      601 tacgattcaa tggttggaac aaaaaatatg acaaccaatc gtccttcgga tgcaggtgta
      661 gtaaatatta aaggaaccga caaagcactt gcaatggtgg tggattgtaa ttcacgctat
      721 gtaaaagctg atcctgaagt aggtacgatg attgccgttt cagaatctgc aagaaacgta
      781 gtttgttcgg gtggtgtgcc aagtgcaatt accaactgtt tgaactttgg aaatccatac
      841 aacccagagt cttactggca atttgttggt gcaatcaaag gaatggcagc tgcttgtact
      901 aaattccaaa caccggtgac aggtggtaac gtttcgttct acaaccaatc ggtaattaat
      961 ggagtagaag agcctgtttt cccaacacca actattggta tgttaggagt tttggaagat
     1021 aaaaacaaaa tgatgacact tgactttaag aaaaaaggcg acttaatttt cttgttaggt
     1081 gaatctgtaa acgatatcgc ttcttcagaa tatttgtatt cttaccatgg tatcaaagct
     1141 tctccagctc catattttga tttagaaaaa gagtttgcag tgcaagaagt tgtaaaagat
     1201 ttaattcaaa gtgagcttat caacgcagca cacgatgttt cggatggtgg tttgttcatc
     1261 actttggcag aaatgtcttt cc
//
LOCUS       DAOQVQ010000593         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_153182_length_1272_cov_2.847165, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000593 DAOQVQ010000000
VERSION     DAOQVQ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153182_length_1272_cov_2.847165"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..>1272)
                     /locus_tag="PKN22_12245"
     CDS             complement(232..>1272)
                     /locus_tag="PKN22_12245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS43520.1"
                     /translation="AHFINGPEVAAFQKEMEDYLKVKHVIPCANGTDALQIALMGLGL
                     KPGDEVITPSFTYVATTEVIALLGLKPVFVEVDKDTFCIDVNQIEAAITPKTKAIVPV
                     HLYGQAAEMDAIMAIAKKHNLFVVEDNAQATGCDYYHADGTTSKTGTIGNIGCTSFFP
                     SKNLGCFGDGGAIFTNDDELAKQMRMIANHGQSKRYYHDVVGCNSRLDTIQAAVLRIK
                     LRKLDEYNNKRRAVADYYDNFFAKYASVKTPVRGAKSKHIFHQYTLQLEGIDRDGLNA
                     FLAEKEVPSMIYYPVPAHRQKMFAAFGSETTHLPITDWLTERVISLPIHTEMEEEQLR
                     LICESVAAFIEK"
BASE COUNT          358 a          250 c          224 g          440 t
ORIGIN      
        1 tagacaatat tgttattgcc gcctggatta ctttgttgtc cttctttttt gcaagacaac
       61 gttaaaatag tagctagtaa aaataaaatg attgtttgta tctttttcat gttttcatat
      121 tattgactac aaatgtaacg attatttttc aaaagttttt tcgtgcaaaa ttttcaacta
      181 aaaaagcctt catcgtgttg acaaaggctt tgttatgaat tgttttaatt tttacttttc
      241 aataaaagct gccacgcttt cgcagattag acgcaattgc tcttcttcca tctctgtgtg
      301 gattggtaac gaaattacac gctctgttaa ccaatctgta ataggtaaat gagttgtttc
      361 acttccaaaa gcagcaaaca ttttctggcg gtgcgctggc actgggtaat aaatcatgga
      421 cggcacttct ttttcagcta aaaatgcatt caatccatct ctatcaattc cttctaattg
      481 caaggtatat tggtgaaaaa tatgttttga tttagcccct ctaactggtg tttttacaga
      541 agcatatttt gcaaagaaat tatcgtaata atcagcaact gctctacgtt tgttgttgta
      601 ttcgtctaat tttctcaatt ttattcttaa caccgccgcc tgaatggtat ctaaacgcga
      661 gttgcaacca actacatcgt ggtagtaacg tttgctttgt ccatggttgg caatcatgcg
      721 catttgttta gccaattcat catcgttggt aaaaatagca ccgccatcac caaaacaacc
      781 caagttttta gatgggaaga aagacgtgca accaatgttt ccaatagttc ccgtttttga
      841 tgttgttcca tcagcatggt agtaatcgca tcctgttgct tgagcgttgt cttcaactac
      901 aaaaaggttg tgttttttag caatcgccat aatagcatcc atttcagctg cttgtccgta
      961 taaatgcacc ggaacaattg cttttgtttt tggcgtaatg gctgcttcaa tttggttaac
     1021 atcaatgcag aaagtgtctt tgtccacttc aacaaaaaca ggtttcaatc ctagtagtgc
     1081 aatcacctca gtagttgcaa cataggtaaa cgaaggagta atcacttcat cgccaggttt
     1141 taatcccaac cccattaatg caatttgaag cgcatcagtt ccgttggcac atggaataac
     1201 gtgttttacc ttgaggtaat cttccatttc tttttgaaaa gcagcaacct ctggtccgtt
     1261 gataaaatgt gc
//
LOCUS       DAOQVQ010000594         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_153759_length_1269_cov_4.677100, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000594 DAOQVQ010000000
VERSION     DAOQVQ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153759_length_1269_cov_4.677100"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKN22_12250"
     CDS             <1..201
                     /locus_tag="PKN22_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HNS43521.1"
                     /translation="DYNADGGAGFFEKIQRQGGSQVPEFLSTHPNPENRIENFHNQKT
                     TKGCTGNKTYATEYKAMVKKLP"
     gene            222..1217
                     /locus_tag="PKN22_12255"
     CDS             222..1217
                     /locus_tag="PKN22_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNS43522.1"
                     /translation="MKSKRTSIDDIAKAVGVSKATVSFVLNGKGDQFNISKAKQKIIH
                     DTAKEMQYVPNFFAKSLRQGSTKTVGLVVSDISNLFYAELSKSIQEQLYENGYNVFIV
                     NTNDDKQLEINLMRELFQRSIDGLILSPSNDVETLKPVFNETSIPVVIADRPIAEDID
                     FVGVDNYAEAKKLVSKFSKKPKKLAVILQTNSMVITLEQRIKGAEAACKELNIPMQLV
                     QLSTEQEKATKDIKKLLEDGYDSFLVLNNVVVLKLLASLRNNQVKVGKDVRLICFDDA
                     EIFDFIEPAITALRQPIKEIGEQAVERMLTRLSKKDAQAGVQNLRECVLIERNSH"
BASE COUNT          459 a          206 c          245 g          359 t
ORIGIN      
        1 gactacaacg ctgatggtgg agctggcttt tttgaaaaaa ttcaacgtca aggtggttca
       61 caagttcctg aatttttaag cacgcaccct aatccagaaa atagaattga gaatttccac
      121 aatcaaaaaa ctacaaaagg ttgtacaggc aataaaacat acgctacaga atacaaagca
      181 atggtgaaaa aattgccata attaatttta agcatactac tatgaagtca aaaagaacat
      241 ccattgacga tattgccaaa gctgttggcg tttcaaaggc aaccgtatca tttgttttga
      301 acggtaaagg cgaccaattc aatatcagca aggcaaagca gaaaataatt cacgatacgg
      361 caaaagaaat gcagtatgtg cctaatttct ttgctaaaag tttgcgccag ggatctacaa
      421 aaaccgttgg tttagtggtg tcggatattt ccaatttgtt ttatgcagaa ttgagtaaat
      481 ccatccaaga gcaactttat gaaaatggtt acaatgtatt tattgtcaac actaatgatg
      541 acaaacaatt ggaaatcaat ttaatgcgtg aattgtttca acgttctatt gacggactta
      601 ttctatcgcc tagcaatgat gtagaaacac ttaagccggt gtttaatgaa acttccattc
      661 cggttgtaat tgcggataga ccaatagcag aagatattga ttttgttgga gttgacaact
      721 atgcagaagc taaaaagtta gtttcaaaat tttctaaaaa acctaaaaaa ttagcagtca
      781 ttttgcaaac caacagtatg gtaattaccc ttgaacaacg tataaaaggt gctgaggctg
      841 cttgcaaaga gttgaacatt cccatgcaat tggtgcaact ttcaaccgaa caagaaaaag
      901 caacaaaaga cattaaaaaa ttgttggaag atggatacga ttctttctta gttttgaata
      961 atgtggtggt tttaaaattg ttggcaagtt taagaaacaa ccaagtaaag gtgggcaaag
     1021 atgttcgact tatttgtttt gacgatgctg aaatttttga ttttattgaa cctgccatta
     1081 cagctcttcg tcagccaatt aaagaaattg gagaacaagc agttgaacgc atgctaacac
     1141 gtttatccaa aaaggatgcg caagcaggtg tgcaaaatct aagagaatgc gtgttaattg
     1201 aacgcaactc gcattaaaaa ctataaaaaa ggagcgtttt gagggaataa aatagcattt
     1261 aatcctgct
//
LOCUS       DAOQVQ010000595         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_155011_length_1263_cov_3.705298, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000595 DAOQVQ010000000
VERSION     DAOQVQ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155011_length_1263_cov_3.705298"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKN22_12260"
     CDS             <1..882
                     /locus_tag="PKN22_12260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNS43523.1"
                     /translation="RKETSDDRLLGFYRFCIGLAKKVLIANTLAVYATQILDGPIAEY
                     STPTVWLGIIAYTFQIYFDFAGYSDMAIGLARIFGFHFPENFNSPYISKSISEFWRRW
                     HMTLGAFMKEYLYIPLGGNRVATQQRLYFNLALVFLLSGLWHGASWNYVLWGAFHGLF
                     LILDRMFLLRILDKLGSVFSIVLTFFIVTVGWAIFRMEDFGQMQLVLYKMFSFDFSST
                     IQLSAEYWLVLLIAIGFSIFTAFNFGKNIEQAVYFQPQATIGRHFVLFGLALLLFIAS
                     LAAITASDFNPFIYFRF"
     gene            882..>1263
                     /locus_tag="PKN22_12265"
     CDS             882..>1263
                     /locus_tag="PKN22_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43524.1"
                     /translation="MNKYSEHIKNGLYIVILMVLLLPQLQSKFKIFSIAPLNGSSEKI
                     ENPYISKTTWLNGSYQEQQEIAAKRSYGFNEVMVRIYNQWNYTFFKKTNTQWVEIGKE
                     GYLYETNYIKAALGLDFIGEDSIQM"
BASE COUNT          368 a          219 c          244 g          432 t
ORIGIN      
        1 agaaaagaaa cctccgacga tcgcctgttg gggttttatc gtttttgcat cggattggct
       61 aaaaaagtac tcattgcaaa tacactagcg gtttatgcta ctcaaatatt agacggacca
      121 attgcagaat attccacacc aacagtttgg ttagggatta ttgcctacac ctttcaaatt
      181 tactttgatt ttgcaggata ttccgacatg gcaatcggct tggcacgcat atttggattt
      241 cattttccag aaaactttaa ttcaccctac atttccaaaa gcatttccga attttggcgc
      301 agatggcaca tgacacttgg tgcttttatg aaagaatatt tatacattcc gcttggagga
      361 aacagagttg caacccaaca gcgtttgtat ttcaacttag ctctcgtgtt tctattgtct
      421 ggtttgtggc acggcgcatc gtggaattat gtgttgtggg gtgcatttca cggattgttt
      481 ttgattttag accgcatgtt tttattgcgt atcttggata aattaggaag tgtattttca
      541 attgtgctta cattttttat cgtaaccgtt ggctgggcca tctttagaat ggaagatttt
      601 ggtcaaatgc aattagtact ttataaaatg tttagcttcg atttctcttc caccattcag
      661 ttaagtgctg aatattggtt ggtattacta attgcaatcg gattttcaat tttcacagct
      721 ttcaatttcg ggaaaaatat tgaacaagca gtatattttc aacctcaggc aactattggt
      781 cgccattttg ttttgtttgg actagcacta ctattattta ttgccagttt agcagccatc
      841 accgcatccg attttaatcc atttatctat ttccgattct gatgaataaa tacagtgaac
      901 atataaaaaa tggattgtac atcgttatat tgatggtgtt gttgttgcct caattgcaat
      961 caaagttcaa gattttttcc attgcgccat taaacggttc atctgaaaaa atagaaaatc
     1021 catacatttc aaaaacaacc tggttaaatg gtagctacca agaacagcaa gaaattgctg
     1081 ccaaacgtag ttatggtttc aatgaggtga tggttcgaat ttacaaccag tggaattata
     1141 ccttttttaa gaaaaccaac acacaatggg ttgaaatagg caaggaggga tatttgtatg
     1201 aaaccaatta cattaaagcg gcattgggat tggattttat tggagaggac agtattcaaa
     1261 tgc
//
LOCUS       DAOQVQ010000596         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_156729_length_1255_cov_3.288333, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000596 DAOQVQ010000000
VERSION     DAOQVQ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156729_length_1255_cov_3.288333"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKN22_12270"
     CDS             complement(<1..439)
                     /locus_tag="PKN22_12270"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNS43525.1"
                     /translation="MKKILVTGGAGYIGSHTVVQLIENGYSPIILDDFRNSQPTVIQN
                     LEKITQQSIPYFAIDICNKEKVYESLKNVAIDSVIHFAAYKAVGESVVQPLLYYHNNI
                     EGLVNVLQWSLENKVKNFIFSSSCTVYGEPKGVKEVNEETASGI"
     gene            592..>1255
                     /locus_tag="PKN22_12275"
     CDS             592..>1255
                     /locus_tag="PKN22_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43526.1"
                     /translation="MRKCTLFLYFFLLFPLISLAQSFNSDYQDGKIWFKLSNETRFEL
                     ELNQNPEKLPLTAIPGFQQIASKYGATNLSKPFHIAKQSKELQLTFEVEFTDFTKVEQ
                     FIKELSALKNVEYAEKVPLYRHCLTTNDPYYSSQWGLTKINAQGAWNYFSTGSTVVVA
                     IVDDAVKRDHPDLAANLWVNPGEIAGNGIDDDGNGYIDDINGYDVGSGDNNPNPTTSS
                     YQH"
BASE COUNT          427 a          203 c          234 g          391 t
ORIGIN      
        1 caattccaga tgcagtttct tcgttcactt ctttaacacc tttgggttcg ccataaactg
       61 tgcaagaaga agaaaatata aagtttttga ctttgttttc aagcgaccat tgcaatacat
      121 tcaccaagcc ttcaatattg ttgtggtaat agagtaaagg ttgcaccacc gattctccca
      181 ccgctttata ggcagcaaaa tgaattacgc tgtcaattgc aacgtttttc aacgattcgt
      241 aaaccttttc tttattgcaa atatcaattg caaaataagg aatagattgt tgggtaattt
      301 tttctaagtt ctgaataact gttggttgtg agttgcgaaa atcatccaaa ataatgggtg
      361 aataaccatt ttcgattagt tgaacaactg tatgtgaacc gatgtatccg gcgccgcctg
      421 taacgagtat ttttttcatg aataaatctt gaacagcaaa tttaaaaatt aaagccaatg
      481 aaacgagata aaatttgaga ttcgtgaaaa agttgaaata aaaatgagct aaaaatttgg
      541 ttgttaggag aaaaaaatag atattggtac agcactttaa actttaaact tatgagaaaa
      601 tgtactttgt ttctttattt cttcttatta tttccactaa tatcgcttgc tcagagcttt
      661 aattcggatt atcaagatgg aaaaatttgg tttaaattat ccaatgaaac acgttttgaa
      721 ttggaattga accaaaaccc agaaaaacta ccattaacag ccattcctgg atttcaacaa
      781 attgcgtcaa aatatggggc aacaaattta tccaaaccct ttcatattgc taagcaatcc
      841 aaagaacttc aactaacatt tgaggtggag tttacagatt ttacgaaagt ggagcaattc
      901 atcaaagagt tatctgcatt gaaaaatgtt gaatatgccg aaaaagttcc gctatacaga
      961 cattgcttaa caactaatga cccctattac agttcgcaat gggggttgac aaaaattaat
     1021 gcgcaagggg cgtggaatta tttttctact ggaagtacgg ttgttgttgc cattgttgac
     1081 gatgctgtga aaagagatca tccagatttg gctgcaaatt tatgggtaaa tcctggtgaa
     1141 attgcaggaa atggaataga cgacgatggt aatggttaca ttgatgatat taatggttat
     1201 gatgttggga gtggtgataa caacccgaat ccaacaacaa gttcatatca acacg
//
LOCUS       DAOQVQ010000597         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_157328_length_1252_cov_3.568087, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000597 DAOQVQ010000000
VERSION     DAOQVQ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157328_length_1252_cov_3.568087"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKN22_12280"
     CDS             complement(<1..571)
                     /locus_tag="PKN22_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS43527.1"
                     /translation="MNYLSVENCTKSYGDRIIFDAISFGINQGQKVAIVAKNGAGKTT
                     LLRSIVGKEIPDEGNIVFRKDIRIAFMEQSEELNPEHTILEEVFSHDLPELNAVKEYN
                     QAMASKDEEAIQVTFEKLSELNAWDIEVRVEQILSVLKLDDTKLKIENLSGGQKKRVA
                     LAKVLIAEPDFLILDEPTNHLDLDMIEWLE"
     gene            complement(581..1057)
                     /locus_tag="PKN22_12285"
     CDS             complement(581..1057)
                     /locus_tag="PKN22_12285"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HNS43528.1"
                     /translation="MIQRIQTLYLIGAITMLVLASFTGELFNYVTDEAVFSFTGFGIE
                     KSLAKDTVVETTSLPILVAFLAVAALAVVVMLSYKKINKQFALAKLLWGMYLLVLIGV
                     VVWNYALAPSQITGKVLSNNYGAAFYFLVIGLHFTHLAFMGIGKDKKTLDSLNRLR"
BASE COUNT          409 a          256 c          179 g          408 t
ORIGIN      
        1 cttccaacca ttcaatcata tccaaatcaa ggtgattggt aggctcatcg aggattaaaa
       61 aatcgggctc tgcgattaac acttttgcaa gcgctactcg ttttttttgt ccacccgata
      121 agttttcaat ttttaatttt gtatcatcaa gtttcagtac ggataagatt tgctcaacac
      181 gcacttcaat atcccaagca ttgagttcgg atagtttttc aaaagtaact tgaatagcct
      241 cctcatcttt agaagccatt gcttggttgt attcttttac agcatttaat tctggtaaat
      301 cgtgcgaaaa aacttcttct aaaatcgtat gttctggatt caactcttca ctttgttcca
      361 taaaagcaat gcgaatatct ttacggaaaa caatgtttcc ttcgtctgga atttctttcc
      421 ctacgatgct gcgcaatagt gtagttttac cagcaccgtt tttggcaaca atggctactt
      481 tttgtccctg attgattcca aacgaaatag cgtcaaaaat aatacgatca ccataggatt
      541 tggtgcaatt ttctactgaa aggtaattca ttttctatga ttaacgcaag cgatttaatg
      601 aatccaatgt ctttttatcc ttaccaatgc ccataaacgc caaatgtgta aaatgcaagc
      661 cgataactaa aaaataaaat gctgcaccat aattattgct cagcactttt cctgtaattt
      721 ggctaggcgc taatgcataa ttccacacaa ctacaccaat caacaccaat aaatacattc
      781 cccacaacaa cttagcaagt gcaaactgtt tgtttatttt cttataactc aacattacaa
      841 ctacagcaag agcagcaacg gctaaaaaag ctactaaaat gggcaacgaa gttgtttcaa
      901 ccacggtgtc ttttgctaat gatttttcaa ttccaaaacc tgtaaaagaa aatacagctt
      961 cgtccgttac gtagttaaac aattctcccg taaaacttgc caaaacaagc atcgtaattg
     1021 ctccaattaa ataaagtgtt tgaatacgtt gaatcataat taatattttt gcacaaaggt
     1081 aacgttttaa ttcaaaaaaa atggccgtct cttttagaaa cagccattta tgtaaagaaa
     1141 tattagctta tttaatcact actttcttag atgctttttt accatccgat gaaactaggt
     1201 taacaaaata aatccctggt ctaaaattag aagcatttac ctctattcta tt
//
LOCUS       DAOQVQ010000598         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_160376_length_1238_cov_2.814877, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000598 DAOQVQ010000000
VERSION     DAOQVQ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160376_length_1238_cov_2.814877"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKN22_12290"
     CDS             <1..238
                     /locus_tag="PKN22_12290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS43529.1"
                     /translation="YYFQKPMIVTNVGGLPEIVPNGKVGYVVAPQSDAIAQAIDLFYS
                     EGKQAIFQQNIAEERKKYEWSNFTNKLISLANKI"
     gene            245..727
                     /locus_tag="PKN22_12295"
     CDS             245..727
                     /locus_tag="PKN22_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNS43530.1"
                     /translation="MDKKYQEWMNQAATKKEEISKQLKNFQKKKPKNLDAIFHTEHQK
                     AFAKIDCLKCANCCKTTSPIFRPVDIKRIAAHVRMTEANFIKTYLRIDEDNDYVLQKS
                     PCTFLNDDNTCSIYEHRPLACREYPHTDRKNMYQIMDLTQKNVSICPAVSFIVMQIVK
                     "
     gene            791..>1238
                     /locus_tag="PKN22_12300"
     CDS             791..>1238
                     /locus_tag="PKN22_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43531.1"
                     /translation="MKTIVLTLFFIFSLIQLTFSQRGKDGNVTISAPNTVVNTYTSLL
                     SNASAGNTTISVTSNALNGAGFSGNLAQGDLIMIYQTQGVWAYLNSSTDYGVNLDWYN
                     SYSWGGSAVPIGYGGSGAYQNAEKYGTVRDYSNAGYYEIREVQSVSG"
BASE COUNT          425 a          211 c          227 g          375 t
ORIGIN      
        1 ctattatttc caaaaaccta tgatagttac caatgttggc ggacttccag aaattgttcc
       61 gaatggaaaa gtaggatatg ttgtggcgcc tcagtcagat gctattgcac aagccattga
      121 tttgttttat agtgaaggaa aacaagcgat ttttcaacaa aatattgcag aggaacgtaa
      181 aaaatacgaa tggtcaaact ttaccaataa gttgatttcg cttgccaata aaatctaagt
      241 tggaatggat aaaaagtacc aagaatggat gaatcaagct gcaactaaga aagaggaaat
      301 ttcgaagcaa ctaaaaaatt ttcaaaagaa aaaaccaaaa aatttagatg caatttttca
      361 tactgaacac caaaaagcat ttgccaaaat tgattgtttg aagtgtgcca attgttgcaa
      421 aaccactagt ccaatattca gaccggttga cattaaacgc atagcagcgc atgtacgcat
      481 gacggaagca aatttcataa aaacctattt acgcattgat gaagacaacg attatgtatt
      541 gcaaaaatcg ccttgtacat ttctcaatga cgacaataca tgtagcatct acgagcaccg
      601 tccgttggct tgtagagaat atccccacac cgatcgcaaa aacatgtatc aaataatgga
      661 tttaacgcag aaaaatgtga gtatttgtcc tgctgtttct tttatcgtca tgcaaattgt
      721 taaataattt gcaattccag caaacttttt tctaatttta tacgttatct ctttattaaa
      781 aataacttct atgaaaacga tagttttaac tttatttttt attttttcac ttattcaact
      841 aactttttct caacgcggaa aagatggaaa cgtcaccata agtgcaccaa atacagttgt
      901 taatacctac acttcgttac tttccaatgc atcagctgga aatactacaa ttagtgtaac
      961 cagcaatgct ttaaatggcg ctggattttc tggaaatttg gcacaaggtg atttaatcat
     1021 gatttatcaa actcaagggg tgtgggccta tttgaattcg agtaccgatt atggagtgaa
     1081 tttagattgg tacaattcgt attcttgggg aggttctgca gttccaattg gatacggagg
     1141 aagtggggct tatcaaaacg ctgagaaata tggaacggta cgcgattatt ccaatgcagg
     1201 atattatgaa attcgtgaag tacaatcagt ttctggct
//
LOCUS       DAOQVQ010000599         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_163685_length_1223_cov_6.586473, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000599 DAOQVQ010000000
VERSION     DAOQVQ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163685_length_1223_cov_6.586473"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKN22_12305"
     CDS             complement(<1..685)
                     /locus_tag="PKN22_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS43532.1"
                     /translation="MNKFAFVSGSSDRIGKAIALELAQMGYHLVLHYNQSQAKVEEVK
                     LAVEALGKKAITVQIDFTQHYDFDALFEQYKAQNLAIEVVVNCASDFIPSSFQDKHAN
                     LLQKELSINFEKAYLLTKAFARTYEKGEIINFIDTKVEKNYSQHLDYILSKKLLKEFT
                     KLSAVHLAPRFRVNAIAPGLVLPPAGKDETYLLNLSKDIPLKTIGNLDDVLKAFRFLM
                     ESKFITGQLI"
     gene            complement(1028..>1223)
                     /locus_tag="PKN22_12310"
     CDS             complement(1028..>1223)
                     /locus_tag="PKN22_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43533.1"
                     /translation="ILNRWGEVVYRFEGNLQAGENKLWNSPATDGVYFYKLTMKELTM
                     DNLQMDNYSLKKEGFVTVVR"
BASE COUNT          372 a          238 c          207 g          406 t
ORIGIN      
        1 aaatgagttg gcctgtaata aatttgctct ccatcaaaaa acgaaaagct ttgagtacat
       61 cgtctaaatt gccaattgtt tttagtggaa tgtctttcga caaatttaat aaatatgttt
      121 catctttacc agcaggcggc aaaaccaatc ctggtgcaat ggcattgaca cgaaaacgag
      181 gtgccaaatg aacggcagaa agtttggtaa attctttcaa caatttttta gacaagatgt
      241 aatccaaatg ttgcgagtag tttttttcca ctttggtatc tataaaattg atgatttctc
      301 ctttttcata cgtgcgtgca aatgctttgg tgagcaaata ggctttttca aagttaatgc
      361 tgagttcttt ttgcagcaag ttggcgtgtt tgtcttgaaa actggatggg ataaaatcag
      421 aggcgcaatt taccaccact tcaattgcta ggttttgtgc tttatattgt tcaaacaaag
      481 catcaaaatc atagtgttgg gtaaaatcta tttgcaccgt tattgctttt ttaccaagcg
      541 cttcaacggc aagcttaact tcttccactt ttgcttgcga ttggttgtaa tgcaacacaa
      601 ggtgatatcc catttgagcc aattccaatg caatggcttt tcctattcta tccgaactac
      661 cagaaacaaa tgcaaattta ttcatcgtta aaactttcta cttacacttt gtgtagcaac
      721 attttgttca aatatacgat taatttttaa agcgaatcag aggagttgag ttatggattt
      781 tttaccataa atgtatctct ttctttgagc gttttttttc gagcaacgaa atgaaaatta
      841 gacaaacgat tgcacggcaa atcctacctt accaccgtta taaaaccttc ttttgttaaa
      901 aaaacgcaat tgtaattttt taaaggattc gagtttccct cctagagagc ctgttccgag
      961 tagcggaaag ggattaaggg aggtgacacc tagagtcaaa aaataatcaa tcagatccca
     1021 attaaaccta ccttaccacc gtcacaaaac cttctttttt caacgaataa ttgtccattt
     1081 gtaaattgtc cattgtcaat tctttcatcg tcaatttata aaaataaacg ccatctgttg
     1141 cgggcgagtt ccacagctta ttttctcctg cttgcaaatt tccctcaaaa cgataaacca
     1201 cctcgcccca acggttgagt att
//
LOCUS       DAOQVQ010000600         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_164830_length_1218_cov_2.947549, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000600 DAOQVQ010000000
VERSION     DAOQVQ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164830_length_1218_cov_2.947549"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /gene="rplM"
                     /locus_tag="PKN22_12315"
     CDS             <1..427
                     /gene="rplM"
                     /locus_tag="PKN22_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNS43534.1"
                     /translation="ANKATVQKEWVLVDATDMVLGRMGSKVAKLLRGKYKPNFTPHVD
                     CGDNVIIINADKVRLTGNKWTDREYMTYSMYPGGQRATTPAEMMAKSPEKLIQKVVKG
                     MLPKNILGAAIMKNLHVYAGSEHPHEAQQPKVIDLNLLK"
     gene            434..820
                     /gene="rpsI"
                     /locus_tag="PKN22_12320"
     CDS             434..820
                     /gene="rpsI"
                     /locus_tag="PKN22_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNS43535.1"
                     /translation="MEVINAIGRRKAAIARVFVTPGTGKIVINKREFDNYFPSTILQY
                     VVKQPLAKLDVTEKYDIKVNLDGGGFKGQAEALRLAIARALVKMNPEDKSALKSAGFM
                     TRDAREVERKKPGQPKARKRFQFSKR"
     gene            984..>1218
                     /gene="rpsB"
                     /locus_tag="PKN22_12325"
     CDS             984..>1218
                     /gene="rpsB"
                     /locus_tag="PKN22_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNS43536.1"
                     /translation="MSRTNFQDLLDAGAHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTVAKIDEAADAMKQIAKSGKRVLFVATKKQAKQ"
BASE COUNT          430 a          211 c          236 g          341 t
ORIGIN      
        1 tgccaataaa gcaacagtgc agaaagaatg ggttttggtt gatgctactg atatggtgct
       61 aggtcgtatg ggttctaaag ttgccaaatt attaagaggc aaatacaaac caaattttac
      121 acctcacgtt gattgtggcg ataatgtgat tattatcaac gccgataaag tgcgtttgac
      181 gggtaataaa tggacagaca gagaatatat gacatattct atgtatccag gtggtcaacg
      241 tgcaacgact ccagccgaaa tgatggcaaa gagtcctgaa aaattgattc aaaaagtggt
      301 aaaaggtatg ctccctaaaa atattttagg tgcagctatc atgaaaaatt tgcatgtata
      361 tgcaggttca gaacacccac acgaagcaca acaaccaaaa gtaattgatt taaaccttct
      421 taaataatcg gatatggaag taataaatgc tataggtaga agaaaagcgg caattgctcg
      481 cgtattcgta actcctggaa caggtaaaat tgtcatcaat aaacgtgagt ttgataacta
      541 tttcccatca acaatccttc aatatgtggt taaacaacca ttagcaaaat tggatgttac
      601 tgaaaaatat gatatcaaag taaatcttga tggcggtggt ttcaaaggtc aagcagaagc
      661 tttgcgttta gcaattgctc gtgctttggt taaaatgaac ccagaagata aatcggcttt
      721 gaagtcggct ggctttatga ctcgcgatgc tcgcgaagtt gaacgtaaaa aaccaggtca
      781 accaaaagct cgtaaacgtt tccaattctc aaaacgttaa tatatgtgtt tggcatcacc
      841 ttgttcgttt ttattcgagc aaggcgatgt tcaacctcat ttgtttagta tctaaatcat
      901 ttagacttac caccgtgtaa ctacttgatg attgcttaac aaaaagttaa aaagaaagta
      961 aacaattttt taaaacaaaa aacatgtcaa gaacaaattt ccaagatcta ttggatgcag
     1021 gtgcacattt tggtcacctt aaacgtaaat ggaatccagc tatggctcca tacattttta
     1081 tggaacgtaa tggtattcac atcatcgacc ttcataaaac agttgctaaa atcgacgaag
     1141 ctgctgatgc aatgaaacaa attgctaaat ctggcaaacg cgtattgttt gttgctacaa
     1201 aaaaacaagc aaaacaag
//
LOCUS       DAOQVQ010000601         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_165493_length_1215_cov_3.918966, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000601 DAOQVQ010000000
VERSION     DAOQVQ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165493_length_1215_cov_3.918966"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKN22_12330"
     CDS             complement(<1..622)
                     /locus_tag="PKN22_12330"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649890.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNS43537.1"
                     /translation="MRVLLLGSGGREHALAWKMAQSSTLDELFIAPGNAGTKNHGKNV
                     DVNPTDFQAVKKFVLEYDIEMVVVGPEEPMVLGIHDFFLNDNEIKHVGVIGPQKEAAQ
                     LEGSKDYAKEFMKRHQIPTAKYGTFTAATLNDGYKFLESMPAPYVLKADGLAAGKGVL
                     ILDKLDEAKKELKNMLVDAKFGNASSKVVIEQFLKGIELSVFVITDG"
     gene            complement(648..1160)
                     /gene="purQ"
                     /locus_tag="PKN22_12335"
     CDS             complement(648..1160)
                     /gene="purQ"
                     /locus_tag="PKN22_12335"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNS43538.1"
                     /translation="MKSIAKLSPIMEEVGKHADKGGYVMGICNGFQILTESGLLDGAL
                     LHNDHQKFVSKNVHLKVGSSNTAITKGLDVTKSYKIPIAHGEGRFYASDDTVKKILDK
                     DQVLFYYCDEEGNVTNESNPNGAVLNIAGICNEKRNVFGMMPHPERASDSELGNEDGR
                     AFFESLLKFC"
BASE COUNT          338 a          259 c          186 g          432 t
ORIGIN      
        1 caccatctgt aattacaaaa acagacaatt caataccttt taaaaattgc tcgatgacaa
       61 ctttagaact tgcatttcca aatttggcat caaccaacat atttttcaat tcttttttag
      121 cttcgtctaa tttatctaaa atcaaaacgc cttttccagc tgccaatcca tctgctttta
      181 acacataggg tgctggcatg ctttctaaaa acttgtagcc atcgttgagt gtagctgctg
      241 tgaaagtgcc gtatttagct gttggaattt gatgacgttt cataaattct tttgcgtaat
      301 ctttacttcc ttctagttga gcagcctctt tttgtggtcc gataacacct acatgtttaa
      361 tctcgttatc gtttaagaaa aagtcatgga tacccaaaac cattggttct tcgggtccaa
      421 ccaccaccat ttcaatatcg tattccaaca cgaatttttt tactgcttgg aagtcagttg
      481 ggtttacatc cacattttta ccgtggtttt ttgtacctgc atttcctgga gcaatgaaca
      541 attcgtccaa cgttgaactt tgtgccattt tccaagccaa tgcatgttct ctacctcctg
      601 atcctaacaa taaaactctc attgctgttt attttttcaa ttgatgatta acaaaatttc
      661 aataacgatt caaagaatgc acgtccgtct tcgtttccta actcactatc agaagctctt
      721 tctgggtgag gcatcattcc aaaaacgttt cttttttcgt tgcaaatacc tgcaatattt
      781 aaaacagctc cgtttggatt agactcgttt gttacattcc cttcttcatc acagtaataa
      841 aacaacactt gatctttgtc taagattttt ttcactgtat cgtcagaagc atagaaacgc
      901 ccttcgccat gtgcaattgg aattttgtat gatttagtta catccaatcc ttttgtaatt
      961 gctgtattgg aagaacctac ttttaaatgc acgtttttag aaacaaattt ttggtgatcg
     1021 ttgtgcaaca aagctccgtc caacaaacca gattctgtta aaatttgaaa tccgttgcaa
     1081 atacccatta catatccacc tttgtcagcg tgttttccaa cttcttccat gataggagaa
     1141 agttttgcaa tggatttcaa attttaacag aatctggttt gttggacgga gctttgttgc
     1201 acaaaccaga ttctg
//
LOCUS       DAOQVQ010000602         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_165779_length_1214_cov_2.604832, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000602 DAOQVQ010000000
VERSION     DAOQVQ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165779_length_1214_cov_2.604832"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKN22_12340"
     CDS             <1..475
                     /locus_tag="PKN22_12340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNS43539.1"
                     /translation="IAHELTHIRNKDVRLLIVTIVFVGIFGVIVDVTFRSLLYGNHRK
                     RDSKDGGGAAILIILLIAVVMYFLSMIFKFALSRSREYMADAGAVEMTKNSLALASAL
                     RKISGNSSFKTTNEEVKELFIDHGETPSNNFFSSISSIFATHPPIEKRIKALEQL"
     gene            488..>1214
                     /locus_tag="PKN22_12345"
     CDS             488..>1214
                     /locus_tag="PKN22_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNS43540.1"
                     /translation="MMRFFYLMLKITLQYTLRIFYPRMKMINSPKEFYGRTIYVSNHA
                     ASFMDPLVVAALRRPIVFFMTRSDVFTPVTKPILWAAHMLPIYREHDGEDTKKKNQEV
                     FDKCADVLSFGRNLLIFGEGFTDDTFIRRLKPVKKGAVRIGFSTLEKINWKKKIYIAA
                     VGCNYSDPNEMRSDLLISTSNKICLNDYKEAYKENPGKVIHDLTKEVERLMQEQITHV
                     EHKEYAPFHEDIMKITRKGMNARC"
BASE COUNT          401 a          215 c          224 g          374 t
ORIGIN      
        1 gattgctcac gaattaacgc atatccgcaa taaagacgta cgtttattga ttgtaaccat
       61 cgtttttgta ggtatcttcg gagtgattgt agatgttaca tttcgttctt tactctacgg
      121 aaatcaccgc aaaagagata gcaaagatgg tggtggagca gcgatattaa taatattgtt
      181 gattgcagta gtcatgtact ttctatcaat gatttttaaa tttgctcttt ctcgtagtcg
      241 cgaatacatg gctgatgcag gagcggttga aatgaccaaa aactcattgg cactggctag
      301 tgctcttcgt aagatttctg gaaattcatc gtttaaaacc acaaatgaag aagtaaaaga
      361 gttgtttatt gatcatggtg aaacaccttc caataatttc ttttcatcca tctcatctat
      421 ttttgcaacc cacccaccca tcgaaaaaag aataaaagct cttgaacaat tatagatttt
      481 aaaaattatg atgcgatttt tctatttaat gcttaaaata acgttgcaat acaccttgcg
      541 catattttat ccacgaatga aaatgattaa ctctccaaaa gaattttatg gaagaacaat
      601 ttatgtaagt aatcacgccg catcatttat ggacccattg gtagttgctg cattaagaag
      661 accaattgtt ttttttatga cgcgctcaga tgtgtttacc cctgttacca aacctatttt
      721 atgggcagcc cacatgttgc ctatatacag ggaacacgat ggagaagaca ctaaaaagaa
      781 aaatcaggaa gtatttgata aatgtgccga cgtgctatcg tttggtagaa atttattgat
      841 ttttggagaa ggttttactg atgacacctt catccgtcgc ctaaaacccg ttaaaaaagg
      901 tgcggttcgt attggttttt caaccctcga aaaaataaat tggaaaaaga aaatatacat
      961 tgctgcagtt ggctgtaatt attccgatcc gaatgaaatg cgcagtgatt tgttgatttc
     1021 aacctctaat aaaatctgcc ttaacgatta caaagaagct tataaagaaa atcctggcaa
     1081 agttatccat gatctaacta aggaagttga acgcttgatg caagagcaaa ttacccatgt
     1141 agagcacaaa gaatacgcgc cttttcatga agatattatg aaaatcactc gaaaaggaat
     1201 gaacgcacgt tgct
//
LOCUS       DAOQVQ010000603         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_166202_length_1212_cov_3.085566, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000603 DAOQVQ010000000
VERSION     DAOQVQ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166202_length_1212_cov_3.085566"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKN22_12350"
     CDS             complement(<1..630)
                     /locus_tag="PKN22_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNS43541.1"
                     /translation="MKSLQYLYKYFIKYKWLLLLGTFFVFASNYFYIKMPLFLKDVID
                     SIINGENFIHVLGFNIPIDTSKASKVALYIGLVYMILVLLKSIFLFFMRQTIIKVSRY
                     IEFDLKNEIYAHYQELGYNFYKENSTGDLMNRVSEDVSYVRTFLGPGIMYNINLVAIF
                     TMIVYQMLNINVWLTFIVLIPLPIMSFLIYKVSEKMNQMSLVVQKEQSGL"
     gene            729..>1212
                     /locus_tag="PKN22_12355"
     CDS             729..>1212
                     /locus_tag="PKN22_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNS43542.1"
                     /translation="MFEVKDISEVNLAENPVLSQMSQFNHEQLVFCNDNETGLKAIIG
                     VHNTVLGPALGGTRIWAYNNEMEALNDVLRLSRGMTYKNSLAGLNLGGGKAVIIGDSR
                     KIKSEALMRRFGKFVNSLSGKYITAEDVGINPQDMVYVNMETDHVVGLPGKSGDPSPV
                     T"
BASE COUNT          431 a          192 c          226 g          363 t
ORIGIN      
        1 gagtccagat tgttcttttt gcactactaa actcatttga ttcatttttt ctgaaacctt
       61 ataaatcaaa aacgacatga tgggcaaggg gatgagcaca ataaaagtta gccacacatt
      121 aatattcaac atctggtaca caatcatcgt gaaaatggca acaaggttaa tgttgtacat
      181 aattcctgga cccaaaaacg tgcgcacata cgaaacatct tccgatacac ggttcatcaa
      241 atcgccagtg ctgttttctt tgtaaaaatt gtatcccaat tcttggtagt gggcataaat
      301 ttcatttttt aaatcaaact caatgtatct agaaacttta ataatggttt ggcgcatgaa
      361 aaacaaaaag atgcttttca gtaaaaccaa aatcatatac accaagccaa tgtaaagtgc
      421 tacttttgag gcttttgaag tgtcgatggg aatattgaag cctaaaacgt gaatgaaatt
      481 ttctccgttg atgatagaat caatcacatc ctttaaaaac aaaggcattt tgatgtaaaa
      541 atagttggat gcaaacacga aaaaagtgcc aagtagcaac aaccatttgt attttatgaa
      601 atatttgtaa agatattgga gtgatttcat tcttaaattc ctaaatttgc actttcaaaa
      661 gtatccgaag catacaaaag taatgaatcg aatacaattg taagtatgtt ttaattaata
      721 attaacgtat gtttgaagta aaagatatct cagaagtaaa ccttgctgaa aatccggttc
      781 tatctcaaat gagccagttc aaccacgaac aattggtttt ttgcaacgac aatgaaacag
      841 gtttaaaagc aattattgga gttcataata cagttttagg tccagcactt ggtggaacac
      901 gtatttgggc ttacaacaat gaaatggaag cattaaacga tgttttgcgt ctttctagag
      961 gtatgacata taaaaattca ttagctggat tgaatttagg aggaggaaaa gcagtgatta
     1021 ttggcgattc tagaaaaatt aaatcagaag cgttgatgcg tcgttttgga aaattcgtaa
     1081 acagtttgag tggaaaatac atcactgcag aagatgttgg tatcaaccca caagacatgg
     1141 tttatgtgaa catggaaaca gatcacgtgg ttggtttgcc aggaaaatct ggagatccat
     1201 cgcctgtaac tg
//
LOCUS       DAOQVQ010000604         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_170097_length_1196_cov_2.856266, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000604 DAOQVQ010000000
VERSION     DAOQVQ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170097_length_1196_cov_2.856266"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKN22_12360"
     CDS             complement(<1..320)
                     /locus_tag="PKN22_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43543.1"
                     /translation="MAQIGDLFVHTDCIHFRGDLPCLPHKKHGYVCQTCPVYTKIETR
                     ILIIKLGAIGDVIRTTPLLRKIRETYPNAKVTWLTQSPAILPKTAIEEVLNYDFKSVL
                     YIQQV"
     gene            complement(375..>1196)
                     /locus_tag="PKN22_12365"
     CDS             complement(375..>1196)
                     /locus_tag="PKN22_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNS43544.1"
                     /translation="LAILPPTFDAQGHRNLDQFYKRETRRSTFDEATYKVELIGFTMN
                     KGDDESFKDDYEMLTVFQIQHALDSMSRKINTIVDGFVSNTENSHEYFQAKNFQKLTD
                     SLKQERVDTVATPIRFNQLTAGEKQLAYQSAIQKIDNRLNAIGVQYQYFTALKQNSNR
                     YWVEFHRKFALTYAIIVLFFVGAPLGAIVRKGGFGAPVVIAALLFMLYFILITVGESL
                     VESQTVSPFLGMWFSSIILTPIALFLMYAAANDAPVMQKDTWKKFFKTITFRNKH"
BASE COUNT          365 a          212 c          231 g          388 t
ORIGIN      
        1 acttgttgaa tgtatagtac cgatttaaaa tcgtaattca acacttcttc aattgctgtt
       61 tttggcaaaa ttgcaggcga ttgtgtcaac cacgttactt ttgcattggg ataagtttct
      121 ctaatcttgc gtagcaatgg agttgtgcga ataacatcgc caattgcacc tagttttatg
      181 attaaaatgc gtgtttctat tttggtgtaa actgggcacg tttggcaaac atagccgtgt
      241 tttttatgtg gtaaacaagg taaatctcct ctgaagtgga tgcaatctgt gtgaacgaat
      301 aaatctccaa tttgtgccat aaaatcttta atcagcgcta aatttacaat ttttattgac
      361 ttttaagaat gaaatcaatg tttgtttcta aaagttatgg ttttaaagaa ttttttccaa
      421 gtatcttttt gcattactgg agcatcgttg gcagcagcat acattaaaaa caaagcaata
      481 ggagtgagga tgatagatga aaaccacatt cctaagaacg gactaacggt ttgcgattct
      541 accaaacttt cgcccactgt aatgagtatg aaatacaaca taaacaacaa ggctgcaata
      601 acaacaggag caccaaaacc accttttctg acaattgcgc ccagtggagc accaacaaaa
      661 aacaacacaa ttatggcgta ggtaagtgca aatttgcgat gaaactccac ccaataacgg
      721 ttagaatttt gttttaacgc agtaaaatac tggtattgaa caccaatggc attgaggcgg
      781 ttatcaattt tttgaatagc tgattgatag gctaattgtt tttctccggc tgttagttga
      841 ttgaagcgaa tgggcgttgc aaccgtatcc actcgttctt gtttcaacga atcggttagt
      901 ttttgaaagt tttttgcttg aaaatattcg tggctgtttt cagtgttact cacaaatcca
      961 tctacaatgg tgttaatttt tctactcatg gagtccaagg catgttgaat ttgaaaaaca
     1021 gtcaacattt cataatcgtc tttaaaactt tcgtcatcgc ctttattcat tgtaaagcca
     1081 atcaattcca ctttataggt agcttcatca aaggtggagc gacgtgtttc acgtttgtaa
     1141 aattgatcca aattacggtg tccttgcgca tcaaatgtag gggggaggat agccaa
//
LOCUS       DAOQVQ010000605         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_170151_length_1196_cov_2.368098, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000605 DAOQVQ010000000
VERSION     DAOQVQ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170151_length_1196_cov_2.368098"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1196
                     /locus_tag="PKN22_12370"
     CDS             <1..>1196
                     /locus_tag="PKN22_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNS43545.1"
                     /translation="VLLLTACAQFKTLEVKKVDDLPTTFNNASNNATEEFIPITLNTY
                     FNDPQLLQLFDRVMKSNPDYQIFQQRILIANSHLKRAKLALIPSLDLSINASGTKYGD
                     HTMEGVGNFDTNLSSNIQEDQKIKRDVTPNYWLGFQTSWEIDVWGRLRHKKRAAQQRF
                     LASQQGINLLKNQLFTDVADLYYQLIALDKKVKIYEANYGVQLKAFEIVESQRAAGKA
                     TELAVQQFGAQSKNMQAEMEQLKIEINSTEKALLSLLGEYSGDLTRGTTFLSNHLALL
                     NQKIHVDSIIHKRPDVAESYFELVASNADAKSARAAFFPKIQFGGNVALHSFSMPTFF
                     NVGSIAWQLLGGLVQPIFNQGQLKQEFYIANRNQEIAFFSYQKNVINAFNELSSLLSQ
                     VAMNQTV"
BASE COUNT          403 a          218 c          208 g          367 t
ORIGIN      
        1 gttttattgc tcactgcttg tgcacaattt aaaaccttag aagtaaaaaa ggtagatgat
       61 ttaccaacta cttttaataa cgcttccaac aatgcaacag aagaattcat tcccattacg
      121 ttgaatacgt atttcaatga tcctcaatta ttgcaattgt ttgaccgagt aatgaaatct
      181 aatccagatt atcaaatttt tcaacaacga attcttattg ccaattctca cctcaaacgt
      241 gctaaattag cgttaatacc gtcgttggat ttaagtatca atgcttctgg aactaaatat
      301 ggcgaccaca ccatggaggg cgttggaaat tttgatacca atctttcttc caacattcaa
      361 gaagaccaaa aaataaaacg cgatgtgact cctaattatt ggttaggttt tcaaacatcg
      421 tgggaaatag acgtttgggg gcgattaaga cacaaaaaaa gagcagctca acaacgtttt
      481 ttggcaagtc aacagggaat taatctactc aaaaaccagt tgtttacaga tgtagctgat
      541 ttgtattacc aattaattgc tttagacaaa aaagtaaaaa tttacgaagc caattatggt
      601 gtgcaattaa aagcatttga aattgtagag tcacaacgtg cagcgggtaa ggcaaccgaa
      661 ttggcagttc agcaatttgg tgctcaatcc aaaaacatgc aagccgaaat ggagcaatta
      721 aaaattgaaa tcaactccac agaaaaagca ttgcttagtt tattaggcga atattcgggt
      781 gatttgacca gaggaacaac atttttatcc aatcatcttg cgttgttgaa ccaaaaaatt
      841 catgtagatt ccatcattca caaacgccct gatgttgccg aaagttattt tgaattagtt
      901 gcttctaatg ccgatgctaa atctgcacgt gcagcatttt ttcctaaaat tcaatttggt
      961 ggtaacgttg cattgcactc attttcaatg cctacatttt ttaatgttgg ttccattgca
     1021 tggcaattat tgggtgggtt ggtgcaacca atttttaatc aaggacaatt gaaacaagaa
     1081 ttttacattg ccaatcgcaa ccaagaaata gcatttttca gctatcaaaa aaatgtaatc
     1141 aatgctttta acgagttaag ttctttgctc tctcaagttg ccatgaatca aaccgt
//
LOCUS       DAOQVQ010000606         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_171501_length_1190_cov_5.824670, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000606 DAOQVQ010000000
VERSION     DAOQVQ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171501_length_1190_cov_5.824670"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKN22_12375"
     CDS             complement(<1..718)
                     /locus_tag="PKN22_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS43546.1"
                     /translation="MNQHIAYFKQLLGDFCLTDEDACQRYSHDETEDIRQIPACVLKP
                     SSVDEVSAILAYCSKNLLPVTPMGARTGLSGGAIPPKNGIALSMERFNKILTIDEKNH
                     QVITEPGVITQVLQDTLKEKGLFYPPDPASKGSCFIGGNVSENSGGPKAVKYGVTKDY
                     VLNLEVVLADGSVIWTGANVLKNATGYNLTQLIVGSEGTLGVVTKIVLKLLPHPTESL
                     LLLVPFFSAKQACEAVAAIYQ"
     gene            complement(792..>1190)
                     /locus_tag="PKN22_12380"
     CDS             complement(792..>1190)
                     /locus_tag="PKN22_12380"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNS43547.1"
                     /translation="KVKGGSLITAELANDYARDVFAYPGDVFHENSSGCNQLIAANKA
                     HLIQNGSDFLKKMGWNNGTTKPVQRSIFPSLSEDEQSIVQLLEQKGTLNLDVISMQLN
                     FPSSKLNVLLFQLEMSGIVALVPGNRCKLV"
BASE COUNT          344 a          263 c          223 g          360 t
ORIGIN      
        1 gttggtaaat tgctgccact gcctcgcagg cttgttttgc agaaaagaac ggcaccaaca
       61 acaacaaact ttcggtagga tgtggaagaa gttttaacac tattttggta accacaccta
      121 acgtaccttc agagccaaca attaactgtg tgaggttgta gcccgtagca tttttaagca
      181 cattggcacc cgtccaaata acagaaccat ctgccagtac tacctctaaa ttcaatacat
      241 aatctttggt aacaccgtat ttcacagctt tgggtccacc tgagttttca gaaacatttc
      301 caccaataaa acacgaacct ttacttgcag ggtcgggtgg ataaaacaat cctttttctt
      361 tgagtgtgtc ttgcaatacc tgcgtaatta cacccggctc ggtaattact tgatggtttt
      421 tttcatcgat ggttaaaatt ttattgaacc gttccatcga aagagcaata ccattttttg
      481 gcggaatagc accaccactc aaccccgttc ttgctcccat aggtgtaacg ggcaacaaat
      541 ttttagagca atacgccaag atagcactca cctcgtcaac cgaagaaggt ttgagcacgc
      601 atgcaggaat ttgccgaata tcttctgttt cgtcgtggct ataacgttgg caagcgtctt
      661 cgtctgtcaa gcaaaaatcg cccaacaact gtttgaaata tgcgatgtgt tgattcataa
      721 agtaaagata gtgcaaagtg aattgctacg gttaagctgt atttattttt ttcaatgcta
      781 cgtttttaat atcacaccaa tttacaacga tttccgggaa ctaacgctac aattccactc
      841 atttctaact gaaacaaaag cacgtttaac ttagaacttg gaaaattcaa ctgcatggaa
      901 atgacatcta aatttagggt gcctttttgc tctagcaact gcacaatgga ttgctcatct
      961 tcggataaac ttgggaaaat gctccgttga accggctttg ttgttccgtt gttccatccc
     1021 atttttttca aaaaatcgct accattttga attaaatgtg ctttgttggc ggcaattaat
     1081 tggttgcaac cacttgagtt ttcgtgaaat acatcaccag gataggcaaa tacatcacgg
     1141 gcataatcgt tggccaattc tgcagtgatg agcgaacctc cttttacctt
//
LOCUS       DAOQVQ010000607         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_172485_length_1186_cov_3.216622, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000607 DAOQVQ010000000
VERSION     DAOQVQ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172485_length_1186_cov_3.216622"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1186)
                     /locus_tag="PKN22_12385"
     CDS             complement(170..>1186)
                     /locus_tag="PKN22_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS43548.1"
                     /translation="PQLEGVAGIDYTYQVNGTTGEYFIPNYLRNSGGVYLIENWQLKK
                     NNEHHYLTGGARFDLAQLNVSGIQNDSVQRFSKTYSNLVGNVGYIYRHNHHLDFVATV
                     GNTYRIPTINELFSNGTHDATYTQGDRNLQPEKALFAQISTRYSSLKWNLSVNIYSYY
                     FFNYIAYQAMNAPVITDHGALPSFEYKQTKANYSGIDLYFDINFTKWLNFQLKGALVR
                     AFDLNTNAHLAYIPADRLQPALKFTHVTAKKDQFSATIGASLVNRQYKAPVEYGFVKA
                     PNGYALMDVSFIYTLNYKGGEVDFIAKMENVFNTKYRDYMNRYRYFANDLGRNIAFKI
                     QISF"
BASE COUNT          362 a          213 c          209 g          402 t
ORIGIN      
        1 ccactagacg aatgggccgt ttttacattt taataatgta agtatgtcaa attttggagt
       61 gcaaatataa tgagttttat tgatatccta tacttttttg ctattttttt tcaaatgttt
      121 tttaattcat tgtttttcaa gcctattttt tgacgcaata atcaatttat taaaaagaga
      181 tttgaatttt aaacgctatg tttcttccca aatcattggc aaaataacgg taacggttca
      241 tgtaatctct gtatttggta ttgaacacat tctccatttt agcgataaaa tcaacctctc
      301 cacctttgta gttgagcgta tagataaacg aaacatccat caacgcatag ccattgggag
      361 ctttcacaaa tccatattct acaggagctt tatactggcg attcaccaaa ctagcgccta
      421 ttgttgccga aaattgatct tttttggctg taacatgcgt aaatttcaag gctggttgca
      481 atctatcagc tggaatgtag gccaaatggg catttgtgtt taaatcaaat gcgcgaacca
      541 acgctccttt caattgaaaa ttcaaccatt ttgtgaaatt gatatcaaaa tacaaatcga
      601 tgcccgaata attggctttt gtttgcttgt attcaaaaga aggcaaagct ccgtggtcgg
      661 taataactgg agcgttcatt gcttggtagg cgatgtaatt gaaaaaataa tacgaataaa
      721 tgttgaccga taaattccat ttcaatgaag aatatcgggt agaaatttgt gcaaacaatg
      781 ctttttcggg ttgtaaatta cgatcgcctt gcgtgtaggt tgcatcgtgc gttccgttgc
      841 taaacaattc gttaattgtt ggaatgcgat aggtatttcc aacagtggct acaaaatcca
      901 aatggtgatt gtgacggtat atgtatccta cattccccac taaattagaa tacgttttag
      961 aaaagcgttg caccgaatcg ttttgaattc cagaaacatt caattgcgcc aaatcaaaac
     1021 gtgctccacc agttaaataa tgatgctcgt tgttcttttt taattgccaa ttttctatca
     1081 aataaacacc acccgagttt cgaaggtagt taggaataaa atattctccc gttgtaccgt
     1141 ttacttgata cgtataatca atacctgcta caccttctaa ttgtgg
//
LOCUS       DAOQVQ010000608         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_173472_length_1182_cov_3.047028, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000608 DAOQVQ010000000
VERSION     DAOQVQ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173472_length_1182_cov_3.047028"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1182
                     /locus_tag="PKN22_12390"
     CDS             13..>1182
                     /locus_tag="PKN22_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS43549.1"
                     /translation="MIPHSSALMNRIEFMGNSLFIPFFLISVGMLVDVKVIFSGWNAW
                     IVAGTLTAVAIVSKWLAAYVTQKTFKFNRPQRQLIFGLSSAHAAATLAIILVGYNVVL
                     GTDANGNPIRLLDDNILNGTIILILITCIVASFATEKASKKLLQLAEEENGTIADSSW
                     ATAEQILIPIDNVGKIERILQFSNLIRNKKSENPISVLTVVSNNSDAESNIVRAKGRL
                     EDIVKEACATETKVKVLTTIDHNVASGIARVSREIVADLIILGWPHQAGFFEKILGDR
                     MDSIIDQTYKTTFICHFEKALTTTKRIVVVVPPYAELEKGFSMWVEKLVRLSQELSIP
                     ILAYCNVETEKSLHKHFQEKKYATQLQVKEFDDWEDILILSKDINKDDLFVLVSAR"
BASE COUNT          377 a          188 c          237 g          380 t
ORIGIN      
        1 gctttaaaca aattgatacc tcattcgtct gcattgatga accgaattga gtttatggga
       61 aattctttgt tcatcccatt tttcttgatt agtgtgggaa tgttggtgga tgtaaaagtt
      121 atttttagtg gttggaatgc ttggatagtt gcaggaacat taacagcagt tgcaattgtt
      181 tcaaaatggc ttgctgctta tgttacccaa aaaactttta aattcaatcg tcctcaacgc
      241 caacttattt ttggtttaag tagtgcacac gccgctgcta cgctggctat tatcttggtt
      301 ggatacaacg ttgttttagg gacagatgct aatggaaatc ctattcgttt gttggatgac
      361 aatattttaa atgggaccat tatcttaata ttaatcacat gtattgttgc atcttttgct
      421 accgaaaaag caagtaaaaa gttattgcaa ttagcagaag aagaaaatgg aacaattgca
      481 gatagttctt gggctacagc agaacaaatt ttaattccaa tagacaatgt tggtaaaatt
      541 gaacgcatct tgcaattctc taacttgatt cgcaataaaa aatctgaaaa tcctatttct
      601 gttcttacgg tagtttccaa caacagcgat gcagaatcga atattgtgcg tgcaaaagga
      661 cgtttggaag acattgtaaa agaagcttgt gcaacagaaa caaaggtcaa agttcttacc
      721 accattgacc acaacgttgc aagtggaatt gcgcgtgtat ccagagaaat tgttgccgac
      781 ctcatcattt tggggtggcc acatcaagct ggattttttg agaaaatttt aggtgacaga
      841 atggatagta ttatcgatca aacgtataaa acaacgttta tctgtcattt tgaaaaagcg
      901 ttgacaacca ccaaacgtat tgttgtagtt gttcctccgt atgctgagtt ggaaaaaggt
      961 ttttcaatgt gggttgaaaa attagtgcgc ctttctcaag aattgagcat tccaatttta
     1021 gcgtattgca atgttgaaac agaaaaatca ttgcataagc atttccaaga aaagaaatat
     1081 gcaacgcaac ttcaagtaaa agaatttgat gattgggaag atattttaat tctgtcaaaa
     1141 gacatcaaca aagatgattt gtttgttttg gtttctgcac gc
//
LOCUS       DAOQVQ010000609         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_175364_length_1175_cov_1.183929, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000609 DAOQVQ010000000
VERSION     DAOQVQ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175364_length_1175_cov_1.183929"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..614
                     /locus_tag="PKN22_12395"
                     /pseudo
     CDS             1..614
                     /locus_tag="PKN22_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138372.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            627..>1175
                     /locus_tag="PKN22_12400"
     CDS             627..>1175
                     /locus_tag="PKN22_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS43550.1"
                     /translation="MNPQKIIEVENLTKKFGDFTAVKSISFHVNKGEIFGFLGANGAG
                     KTTAMKMLIGISNPTSGNAIVAGYNVQTQSELVKKSIGYMSQKFSLYDDLTVQENITF
                     FGGIYGLSKNQIKSKTETLIDNLQLGNITKTKVGDLPLGWKQKLAFSVALLHEPKIVF
                     LDEPTGGVDPITRRQFWELIYAE"
BASE COUNT          440 a          152 c          215 g          368 t
ORIGIN      
        1 aaatttagaa ttttttgcaa ctatttttgg tacgacaatc gaagaaaatt acgatttaat
       61 cgaagatatt tacgttcaaa ttgaaccttt taaaaataga agagcaggag cattatctgg
      121 tggaatgaaa caaaaactag cactttgttg tgccttgatt cataaaccta aagttttgtt
      181 tttagacgaa cctacaactg gagttgatcc tgtttcaaga aaggaatttt gggaaatgct
      241 caagcgatta aaacaaaaag gaattacaat tttagtatca actccttata tggacgaagc
      301 ggctttatgt gatagaattg cgttaattca agaaggaaaa atattaaaaa tagattcgcc
      361 tgaaaacatt gtgaattcgt atgaaaaagc aattttcgaa gtaaaatcag atacgatgta
      421 tcaattaatt gaagatttaa aagcgtttcc ctcacgttat agtgtgtatg cttttggtga
      481 gtttgtacat tatactgaaa ggtgatttat ttcaaattga aaatttaatg agttatttaa
      541 ccgaaaaagg acatcagaat attgtaattc aaaaagcaaa aaccactatt gaagatgttt
      601 ttatggattt gtaaaacaaa cataaaatga acccacagaa aatcatagaa gtagaaaatt
      661 taaccaaaaa atttggcgat tttacagccg taaaaagtat ttcatttcac gtgaataaag
      721 gtgaaatctt tggtttttta ggtgcaaatg gtgctggaaa aactactgct atgaaaatgc
      781 ttattggtat ttcaaatcct acaagtggta acgcaattgt tgcaggttat aatgtgcaaa
      841 cgcaaagtga attggtgaaa aaaagtattg gttatatgag ccagaaattt tcactttatg
      901 acgatttaac ggtacaagaa aacattacat tttttggtgg catttatggt ttatcaaaaa
      961 atcaaattaa atccaaaaca gaaactttaa ttgacaatct ccaattggga aatatcacaa
     1021 aaactaaagt tggcgatttg cctttggggt ggaaacaaaa attagcgttt tcggttgcat
     1081 tattacacga acctaaaatt gtttttttgg acgagccaac aggaggcgtt gatccaatta
     1141 cgaggagaca attttgggaa ttaatttatg ccgaa
//
LOCUS       DAOQVQ010000610         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_175656_length_1173_cov_3.470483, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000610 DAOQVQ010000000
VERSION     DAOQVQ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175656_length_1173_cov_3.470483"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKN22_12405"
     CDS             <1..319
                     /locus_tag="PKN22_12405"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNS43551.1"
                     /translation="NVHRVSSQQRKQLHVSAVFTNNFVNHLLYLAQQHLKANQLDEQL
                     LVPLLKETIAKAEILGPYEAQSGPARRNDVHTIETHLHQLTGSAQKIYADITESILKT
                     YHK"
     gene            316..834
                     /locus_tag="PKN22_12410"
     CDS             316..834
                     /locus_tag="PKN22_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNS43552.1"
                     /translation="MISYKERLNKITTFIFDVDGVLTNGDVIILQNEMVRVLNSRDSY
                     ALRYAAQMGYRVFVITGGTSEIVKERLLELGVHEVFLRSSNKLNVYNKLLEKYQLQDE
                     EILYMGDDIPDYRVMERVGCATCPQDAAVEIKSIAHYQSPFFGGKHAVRDVIEQTMRV
                     HGKWFSEAAHEW"
     gene            897..>1173
                     /locus_tag="PKN22_12415"
     CDS             897..>1173
                     /locus_tag="PKN22_12415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS43553.1"
                     /translation="MKAGKNTVVSLDYTLKNNAGHVMDSSEGHEPLVYLHGAGFLIPG
                     LEREIEGMEKGQTKTVVVQPEHAYGIRQDELLYVVSKSGFQGEEEMVV"
BASE COUNT          387 a          205 c          254 g          327 t
ORIGIN      
        1 aaacgtgcat cgtgtttcat cacaacaacg taaacaattg catgtttcgg ctgtttttac
       61 caataatttt gtcaatcatt tattgtattt ggcacaacaa catttgaaag ccaatcaatt
      121 ggacgagcaa ttattggttc ccttgctgaa agaaaccatt gccaaagccg aaattcttgg
      181 tccgtatgaa gcacaaagcg gtccggcacg tagaaacgat gtgcacacaa tagaaacaca
      241 cctccaccaa ttgacaggaa gtgcgcaaaa aatttatgca gatattaccg aaagtatttt
      301 aaaaacatat cacaaatgat ttcctacaaa gaacgtttaa acaaaattac caccttcatt
      361 tttgacgttg atggcgtatt gacaaatggc gatgtaatca ttctccaaaa cgaaatggtg
      421 cgtgttttaa attcgcgcga tagttatgcc ttgcgttacg ctgcacaaat gggctaccga
      481 gtttttgtaa ttacaggagg cacgtcagaa attgtaaaag agcgtttgct agaattaggc
      541 gttcatgagg tgtttttacg ttcgtccaac aagctcaatg tttacaacaa gttattggaa
      601 aaataccagt tgcaagatga agaaatctta tacatgggtg atgacattcc cgactatcgc
      661 gtgatggagc gtgtaggttg cgctacttgt ccgcaagatg ccgccgttga aataaaatcc
      721 attgcccatt atcaatctcc gttttttgga ggtaaacatg cagtgcgcga tgttattgag
      781 caaaccatgc gtgtacacgg aaaatggttt tcggaagcag ctcacgaatg gtaaaaagtt
      841 tatttaaaaa aagttaatag tagtaataag ttcatttaat ctcgtaaatt tggaatatga
      901 aagcagggaa aaacacagta gtttcgttgg actacacatt aaaaaacaac gctggtcacg
      961 tgatggattc atctgagggg cacgagccat tggtttattt acacggagca ggatttttga
     1021 ttccaggatt ggagcgagaa attgaaggca tggaaaaagg acagacaaaa acagttgttg
     1081 ttcaaccaga acatgcttat ggaatcagac aagatgaatt gttgtatgta gtttctaaaa
     1141 gcggtttcca aggagaagaa gaaatggttg ttg
//
LOCUS       DAOQVQ010000611         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_177414_length_1166_cov_3.737174, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000611 DAOQVQ010000000
VERSION     DAOQVQ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177414_length_1166_cov_3.737174"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKN22_12420"
     CDS             <1..1101
                     /locus_tag="PKN22_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009420753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNS43554.1"
                     /translation="YREEVDEQTGFTEKVIIESRDKKKNPAIHIIDPKTKEVLREYSL
                     PVDAHISIKEGEVLEAGKVLAKIPRKSGKSGDITGGLPRVTELFEARNPSNPAVVSEI
                     DGIAEYGKVKRGNREISITSKSGEVRKYLVPLSKHILVQENDFVRAGQPLSDGAITPN
                     DILNIQGPTKVQEYIVNEIQEVYRLQGVKINDKHFEVIVRQMMLKAEVVDAGDTRFLE
                     GQSVHKADIMEENDEIFGKKVVVDAGDSTVLKPGQIVTARKLRDENSQLRRADKKLVE
                     AREAIPATSTQLLQGITRASLQTQSFISAASFQETTKVLNEAAVAGKQDFLLGLKENV
                     IVGHLIPAGTGVRNFQKVIVGDKEQMEEISKK"
BASE COUNT          417 a          193 c          253 g          303 t
ORIGIN      
        1 taccgtgaag aagtcgatga gcaaacagga tttacagaaa aggttattat cgagtcgcgt
       61 gataagaaaa agaaccctgc aattcatatc attgatccta aaacaaaaga agttttaaga
      121 gaatattcat tgcctgttga tgcacacatt tctattaaag aaggtgaagt gttagaagca
      181 ggtaaagtat tagcaaaaat cccacgtaag tctggtaagt ctggggatat caccggaggt
      241 ttaccacgtg taacagagtt attcgaagca cgtaaccctt ctaacccagc ggtagtatca
      301 gaaattgatg gtattgcaga atacggtaaa gtgaagagag gtaacagaga aatttcgatt
      361 acatctaaat caggtgaagt tcgtaaatac ttagttccac tttctaaaca catcttggta
      421 caagaaaatg actttgttcg tgctggacaa ccattatcag acggagcaat tactccaaac
      481 gatattttga acatccaagg acctacaaaa gtgcaagaat acattgtaaa tgaaatccaa
      541 gaggtttacc gtttacaagg ggtaaaaatt aacgacaaac acttcgaggt tattgtacgt
      601 cagatgatgt tgaaagccga agttgttgat gcgggagata ctcgtttctt ggaaggacaa
      661 tctgttcaca aagcagatat catggaagaa aacgatgaaa tctttggtaa gaaagtagta
      721 gttgacgctg gagattcaac tgtgttgaaa ccaggacaaa ttgttaccgc tcgtaaattg
      781 agagatgaaa actcacaatt gagacgtgct gacaagaaat tggttgaagc aagagaagct
      841 atccctgcaa catctactca gttgttacaa ggtatcacaa gagcatcatt gcaaacacaa
      901 tcgtttattt ctgcggcatc gttccaggaa acaacgaaag tattgaatga agctgctgtt
      961 gctggtaaac aagatttctt gttaggatta aaagaaaacg ttatcgttgg tcacttaatc
     1021 cctgctggta caggtgttcg caatttccaa aaagtaattg ttggagacaa agaacaaatg
     1081 gaagaaattt caaaaaaata atctttcaga taggcataaa gaaaggcgct caattgagca
     1141 cgaatggtcg gaagaaattt cttccg
//
LOCUS       DAOQVQ010000612         1154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_180577_length_1154_cov_4.481347, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000612 DAOQVQ010000000
VERSION     DAOQVQ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180577_length_1154_cov_4.481347"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKN22_12425"
     CDS             <1..662
                     /locus_tag="PKN22_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43555.1"
                     /translation="DTTIQSINPDAIAYIDTFKLVPIQLIHKQLKSNAKQSLVFKTPA
                     FSKSDSLDTTTIIEQLAVNFTYHKLGELSKNKKLMASSKSEMSNLATLLFPDSILPKK
                     ITSDSLNSWFKQALNNNHQWIYFEAIEWLEQLYITVENPKIHKSKAKFQALVELQLEN
                     GTEMLERISMHQDFEPLALLMNDLISIIDCKYYTFNPVELKEEVVKARENCYFLPKSA
                     K"
     gene            664..>1154
                     /locus_tag="PKN22_12430"
     CDS             664..>1154
                     /locus_tag="PKN22_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS43556.1"
                     /translation="MSIYQEIIDAVNNATNIVITSHKSPDGDSIGSSLGWSRFIQKMG
                     KNVTVCHPDKAATSFNWLDDLKDILLFETHPEEVNKAVQNADLLFCLDYNHLSRVGSE
                     MQQVLEKYKGKKILIDHHLSPSVDAIISLSDTNVCSTSQLIFDVIDYSNKEHLLDAHI
                     ATP"
BASE COUNT          409 a          203 c          187 g          355 t
ORIGIN      
        1 aagatacaac catccaaagt atcaaccctg atgctattgc atacattgat acttttaaac
       61 tggttccaat ccaactcatt cataaacaat tgaaaagcaa tgcgaaacaa tcattagttt
      121 ttaaaacacc cgcatttagc aagagtgatt ctttggatac aaccactatt attgaacaat
      181 tagcggttaa ttttacgtat cataaattgg gagaactgtc taagaataag aagttaatgg
      241 ctagctcaaa aagcgaaatg agtaatttgg caactttgct ttttccagat tccattctgc
      301 ctaaaaaaat tacgtctgat tcattaaatt cttggttcaa acaagcattg aacaacaatc
      361 atcaatggat ttattttgaa gcaattgaat ggttagaaca attatacata accgttgaaa
      421 atccaaaaat tcataaaagc aaagcaaaat tccaggcgtt ggttgaactt caattagaaa
      481 acggaacaga aatgttagag cgcatttcca tgcatcaaga ttttgaacct ttagcattgt
      541 taatgaacga tttaatttca atcatcgact gcaaatatta tacatttaac ccagtagaat
      601 taaaagagga agttgtaaaa gcacgtgaaa attgctattt tcttccaaaa tctgcaaaat
      661 aacatgtcaa tttatcaaga aattatcgat gcggtcaaca acgcaacaaa cattgtaatt
      721 acttcacaca aatcgcccga tggagattct attggatctt cattaggttg gagccgtttt
      781 atacaaaaga tgggtaaaaa tgttaccgtt tgtcaccccg acaaagctgc cacttcgttt
      841 aattggttag acgatttgaa ggatattctt ttgtttgaaa cccatccaga agaagtaaac
      901 aaagctgttc aaaatgctga tttacttttt tgtttggatt acaaccattt atcgcgtgtt
      961 ggaagtgaaa tgcaacaggt tttggaaaag tacaagggta aaaaaatact aattgaccat
     1021 cacctctcac cttctgtaga tgcaattatt tctttgagcg ataccaatgt ttgctccaca
     1081 agccagttga tttttgatgt aattgattat tcaaacaaag aacatttgtt ggatgcccac
     1141 attgcaacgc cttt
//
LOCUS       DAOQVQ010000613         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_181908_length_1149_cov_4.308958, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000613 DAOQVQ010000000
VERSION     DAOQVQ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181908_length_1149_cov_4.308958"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PKN22_12435"
     CDS             <1..1087
                     /locus_tag="PKN22_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43557.1"
                     /translation="MSNASDGVFYLDNSRNFEKNQSFWYIRTMLPGLFHELKDIYQGN
                     VVLGDIYLNEKSIEFKVSSNYGDKLGSVYQQLNDTKFDKNILKYIDKNSSSFFTYNVN
                     LRNAYEKAYEVLVPILDKEDNEEIAASLLVLDLLNEYVNKDALFGSVKGSVFGSFNGI
                     KKVKTKKIIFDYDEDTFEYTEHEVEAEEEMPIFTFGLSSERTDIFDKVLSRLTKINKN
                     LEKKEGYWMVSNAILESAPLYIVNKNGLLIFSNDEDFAINHNNGYGINAITGKKAKEI
                     KSNGFMFAEVDWGKTIDKLPRDLFNGEQNEIIDAMRGKGGFMKLTSTKTTRKNTKFNL
                     VYTFDSKYENSGKYILDFVNSIYLISK"
BASE COUNT          397 a          165 c          237 g          350 t
ORIGIN      
        1 aatgagcaat gcttcggatg gtgtatttta cttggataac tctagaaatt ttgagaaaaa
       61 tcaaagtttt tggtacatcc gaactatgtt gccaggattg tttcacgaac ttaaagacat
      121 ttatcaaggc aacgtagttt tgggcgacat ttacttgaac gaaaaaagca tagagtttaa
      181 ggtttcatct aactatggcg ataaattagg ttcggtttac caacaattga acgacaccaa
      241 atttgataaa aacatcttaa aatacattga taaaaacagt tcttcgtttt ttacctacaa
      301 tgttaatttg agaaatgcct acgaaaaggc ttatgaagtg ttggtgccaa ttttggataa
      361 agaagacaac gaggaaattg cagcttcgtt attggtgtta gaccttttga atgaatacgt
      421 gaacaaagat gcgctttttg gaagtgtgaa aggaagtgtt tttggttcat ttaatggtat
      481 caaaaaagta aaaactaaga aaattatttt tgattacgac gaagatacgt ttgaatatac
      541 cgaacatgag gtggaggctg aagaagaaat gccgattttc acgtttggac tttcatcgga
      601 gcgtacggat atttttgaca aagtgttgag tcgcctaaca aaaatcaata aaaacttaga
      661 aaaaaaagaa ggttattgga tggtgagcaa tgccatttta gaatcagcac ctctctacat
      721 cgttaacaaa aacggtttgt tgattttctc aaacgacgaa gattttgcca tcaatcacaa
      781 caatggttac ggcatcaatg caattacggg taaaaaagcc aaagaaatta agtcgaatgg
      841 gtttatgttt gccgaagttg attggggaaa aaccattgat aaattgccac gcgacctatt
      901 taacggtgag caaaacgaaa tcattgatgc catgcgtggt aaaggtgggt ttatgaaatt
      961 gacctccacc aaaacaacca gaaaaaatac aaaattcaac ttggtgtata cgtttgacag
     1021 caagtatgag aattctggaa aatacattct tgattttgta aatagcattt atttgatttc
     1081 aaaatagggg caaatgttgc gtaaactgac gcttattttt gtgttggctg cgttatttgg
     1141 tgcattttt
//
LOCUS       DAOQVQ010000614         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_185055_length_1138_cov_2.466297, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000614 DAOQVQ010000000
VERSION     DAOQVQ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185055_length_1138_cov_2.466297"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKN22_12440"
     CDS             <1..760
                     /locus_tag="PKN22_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNS43558.1"
                     /translation="ETLDSEVVDRTLTEVEIDSNVSVNPETGEVLNKEQEKQRISVIF
                     ILFAFAVFFWAGFEQAGSSLSLYTDKFIDRSIGGFEIPTSWFQSVNPIFIVALAPLFA
                     IFWNSKLGKRLSTPVKMGVGMIILGLGFLFMLGAVGERAANGNIEDEANKAALMWLIM
                     TYLLHTIGELCLSPVGLSVVTKLAPPKLASILMAVWMLSSSVANFIGGYLASVVETMG
                     AGQIFTFIAIFVMICGLLLISLNKFIGKLMHGVK"
     gene            978..>1138
                     /locus_tag="PKN22_12445"
     CDS             978..>1138
                     /locus_tag="PKN22_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS43559.1"
                     /translation="MARELEQIQNFEGKYPKQIWGLFFSEMWERFCFYGMRGVLTYFM
                     ITELILADG"
BASE COUNT          336 a          188 c          226 g          388 t
ORIGIN      
        1 tgaaacattg gacagtgaag tagttgacag aacgttaaca gaagttgaaa ttgactcaaa
       61 cgtatctgta aaccctgaaa cgggtgaagt tctgaacaaa gagcaagaga aacaacgtat
      121 ttctgttatc ttcatcttat ttgcatttgc cgtgtttttc tgggctggtt ttgaacaagc
      181 tggttcatcg ctttcacttt ataccgataa gtttatcgac cgttcaattg gtggttttga
      241 aatccctact tcttggtttc aatcagtgaa tccaattttt attgtggcat tggcaccatt
      301 gtttgctatt ttctggaatt ctaaattagg aaaacgctta tctactcctg taaaaatggg
      361 tgtgggaatg atcatcttag ggttaggatt cttattcatg cttggagctg ttggcgaaag
      421 agctgcaaac ggaaacattg aagacgaagc caataaagct gctttgatgt ggttgattat
      481 gacttattta ttgcacacca ttggtgagtt gtgtttatcg ccagttggac tttctgtggt
      541 aacaaaatta gcaccgccaa aattagcttc tatcttgatg gctgtttgga tgttatcttc
      601 ttctgttgct aactttattg gaggttattt ggcttcagtt gtggaaacaa tgggtgctgg
      661 acaaatcttc accttcattg caatatttgt aatgatttgt ggattgttac ttatctcgct
      721 caataaattt attggaaaat taatgcacgg tgtgaaataa accacacacc ctccatctta
      781 tagaaacatc ctagcagttt tgttaggatg tttttttatg cctaaaaagg atattttacg
      841 catcaataac taaaaaatat tctggatagt acattttttt aaacagaata attacattta
      901 aagtcgcttg taaacaatgc aagataaaaa ctataaaaat gagcaacact actgaacaat
      961 ccattgatgc ggctgcaatg gcacgcgaat tagaacaaat tcaaaatttt gaaggcaaat
     1021 atccaaaaca aatttgggga ctttttttct ccgaaatgtg ggaacgtttt tgtttttatg
     1081 gtatgcgcgg cgtactcacc tacttcatga ttaccgagtt aattcttgcc gatggtag
//
LOCUS       DAOQVQ010000615         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_185393_length_1137_cov_2.213494, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000615 DAOQVQ010000000
VERSION     DAOQVQ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185393_length_1137_cov_2.213494"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..719
                     /gene="rpsD"
                     /locus_tag="PKN22_12450"
     CDS             114..719
                     /gene="rpsD"
                     /locus_tag="PKN22_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNS43560.1"
                     /translation="MARYTGPKSKIARKFGEPIFGPDKAQARRNFPPGQHGNTRKRKT
                     SEYGVQLKEKQKAKYTYGVLEKQFRNLFEKAQAHSGITGEILLKLLECRLDNVVYRLG
                     IAKTRSAARQLVSHKHITVDGEVVNIPSYSVKQGQVIAVREKDKSMSVIVDSLNGFNH
                     SKYPWLEWDNTAMAGKFLHTPERADIPENIKEQLIVELYSK"
     gene            729..>1137
                     /locus_tag="PKN22_12455"
     CDS             729..>1137
                     /locus_tag="PKN22_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNS43561.1"
                     /translation="MAILAFQKPDKVIMLEANDFQGKFEFRPLEPGFGITIGNALRRI
                     LLSSLEGYAITSIKIEGVDHEFATIPGVIDDVTNIILNLKQVRFKATHDKADGEKLTL
                     VVSGREVVKAGDLNSLLTNFEVLNPELVLCKMDV"
BASE COUNT          378 a          189 c          226 g          344 t
ORIGIN      
        1 cggatttttt ttgatatgac tgcattttca aacctctctg cagtcgcggc tgtcacaaat
       61 ttgaaagtag aaaaaggatg aaacatcaat tttaaaaaaa taaattttaa aaaatggcaa
      121 gatatactgg accaaaatca aaaattgctc gcaaatttgg tgaaccaatt ttcggacctg
      181 ataaagctca agcccgtaga aatttccctc ctggacaaca cggtaatact cgtaaacgta
      241 aaacgtctga gtatggtgtt cagttgaaag aaaaacaaaa agctaaatat acctacggcg
      301 tattagaaaa acaattccgc aacttgtttg aaaaagctca agcacactct ggtattacag
      361 gtgaaatttt gttgaaattg ttagaatgtc gtctcgataa cgttgtttat cggttaggta
      421 tagcaaaaac acgttctgct gctcgtcagt tggtatcaca caaacacatc acggtagatg
      481 gagaagtggt aaatatacct tcttattcag taaaacaagg acaagtgatt gctgttcgtg
      541 aaaaagataa atcaatgtct gttatcgttg attctttgaa tggctttaat cacagcaaat
      601 atccttggtt agaatgggat aacacagcta tggctggtaa gttcttacac acgccagaac
      661 gtgcagatat tcctgaaaac attaaggaac aattaatcgt tgaattgtac tctaaataat
      721 tgtagaatat ggctatatta gcatttcaaa aaccagacaa ggtaatcatg ttggaagcga
      781 acgactttca aggtaaattt gaatttcgtc cgttggaacc cggatttggt attactatag
      841 gtaatgcttt gcgccgcatt cttctctctt ctttggaagg atatgcaatt acttcgatta
      901 aaattgaagg agttgatcac gaatttgcta ccatcccagg agtaatcgat gatgttacaa
      961 atattatcct caacttgaaa caagttcgtt ttaaagctac tcacgataag gctgatggcg
     1021 aaaaattgac attagttgtt tcgggtcgcg aagtggtaaa agcaggtgac ttaaattcgt
     1081 tgttgaccaa ttttgaagtg ttgaatcctg aacttgtttt gtgcaagatg gatgttt
//
LOCUS       DAOQVQ010000616         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_186681_length_1132_cov_4.228412, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000616 DAOQVQ010000000
VERSION     DAOQVQ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186681_length_1132_cov_4.228412"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKN22_12460"
     CDS             complement(<1..1042)
                     /locus_tag="PKN22_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNS43562.1"
                     /translation="MAYTIKKDALKFLENYLNNASPTGFESEGQKLWLDYLKPYIDSY
                     FTDNYGTVVGVINPEAEYKVVIEAHADEISWFVHYITKEGFIYLVRNGGSDHMIAPSK
                     RVNIHTEKGIVKGIFGWPAIHTRHAKDEAPSMKNIFLDVACSSKEEVEALGIHVGCVV
                     TFEDEFMVMNKNRFVGRALDNRIGGFMIAEVARHLKESKTKLPFGLYIVNSVQEEIGL
                     RGAEMISRRIKPDVAICTDVCHDTNTPMIDKIEQGDHKSGDGPVLTYGPAVQNNLLKQ
                     IIATAEKNKIPFQRAAVSRSTGTDTDAFAYSQDGVTSALISLPLRYMHTTVETCRKED
                     VENCIRLIFETLK"
BASE COUNT          309 a          242 c          200 g          381 t
ORIGIN      
        1 tttttagggt ttcaaaaatc aatcggatgc aattttccac gtcttctttg cgacatgttt
       61 ctacagttgt gtgcatgtaa cgtaaaggca atgaaatcaa tgcagaagtt actccgtctt
      121 gcgaataagc aaatgcgtcg gtgtctgtac cagttgaacg tgatacagca gcacgttgga
      181 acggaatttt gtttttctcg gcggttgcaa taatttgttt caacaagttg ttttgtaccg
      241 ccggaccata ggtcaacacc ggaccatcac ccgatttatg gtctccttgt tcaattttgt
      301 caatcattgg tgtgttggtg tcgtggcaaa catctgtaca aatcgcaaca tctggtttaa
      361 ttctgcgaga aatcatttcg gcacctcgca aaccaatttc ttcttgcacc gagttaacga
      421 tatacaaacc aaagggcaat tttgttttag attcttttaa atggcgcgcc acttcagcaa
      481 tcataaaacc accaatgcgg ttgtcgagtg cacggcccac aaaacggttt ttgttcatca
      541 ccataaattc gtcttcaaaa gttaccacac aaccaacgtg gatacccaaa gcttctacct
      601 cttctttgct agagcaagca acatctaaaa atatgttttt catgctcgga gcttcgtctt
      661 tagcgtgacg tgtatgaatg gctggccaac caaaaattcc ctttacaatt cctttttcgg
      721 tatgaatatt cacccttttt gaaggtgcaa tcatgtggtc agaaccaccg ttgcgcacca
      781 agtaaataaa accttctttg gtgatatagt gcacaaacca tgatatttca tcggcatgcg
      841 cctctatcac tactttgtat tctgcttctg ggttgataac tcccacaact gttccataat
      901 tatccgtaaa atacgaatca atgtatggtt ttaaataatc taaccacaat ttttgtcctt
      961 ccgattcaaa tcctgttgga cttgcattgt tcaagtaatt ttctaaaaat tttaaggcgt
     1021 cttttttaat tgtatatgcc attttattgc tattttaaag taattcgttt gctaagttag
     1081 ctaattctga gcgttctcct ttctctaatt tgatatgagc aaataattct tg
//
LOCUS       DAOQVQ010000617         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_190536_length_1118_cov_4.042333, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000617 DAOQVQ010000000
VERSION     DAOQVQ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190536_length_1118_cov_4.042333"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..985
                     /locus_tag="PKN22_12465"
     CDS             44..985
                     /locus_tag="PKN22_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNS43563.1"
                     /translation="MLTEKLALGIDIGGTNTAFGVVSEDGKILLEESVSTNDFETADK
                     LANYIYSILSKQSYFSQLIGIGIGAPNGNRFKGNIEFAPNLIWKGIVPLAQIFEKQFG
                     LLCIVDNDANIAAVGEMKFGAARNMKNFVTITLGTGLGSGVIVDGKLMHGAFGFAGEY
                     GHVRVEKDGRLCGCGRKGCLETYVSATGVVRSINEMPSDKKASSTLNAIAHPTSLDVV
                     EAANKNDAFAIEILDYTAKMLGEALADFMCFSNPEAFVLFGGMAKVGQAFAQQVEAYM
                     NAAALKIYQDKTKVVISTIQDHNAAILGAAALVFHQD"
BASE COUNT          368 a          163 c          251 g          336 t
ORIGIN      
        1 ttctagaaca gttttatact gagtggaata ataaattaaa gtaatgttga cagaaaagct
       61 agctttagga attgatattg ggggaaccaa cactgcattt ggtgttgtga gtgaagatgg
      121 aaaaattctg ttagaagaat ctgtgtcaac caatgatttt gagactgctg ataaattggc
      181 taactacatt tattccattc tttccaaaca gtcgtatttt tcacaattaa ttggtattgg
      241 aattggtgca ccaaatggca accgatttaa aggaaacatt gaatttgcac ccaatttaat
      301 ttggaaaggc atagtgccac tggctcaaat ctttgaaaaa caatttggct tgttgtgtat
      361 agttgataac gatgcaaata ttgctgctgt tggtgaaatg aagtttgggg ctgcacgcaa
      421 tatgaaaaat tttgtaacta ttactttagg cactggacta ggaagtggag tcatagtgga
      481 tggtaaattg atgcatggtg cttttggctt tgcaggagaa tatggccatg tgcgagtaga
      541 aaaagacgga agattgtgtg gatgtggcag aaaaggatgt ttagaaacct atgtttcggc
      601 aacaggagtg gtaagaagca tcaacgaaat gccttctgat aaaaaagcga gttcaacact
      661 caacgcaatt gcacatccaa ctagtttaga tgtagtagaa gcagccaata agaacgatgc
      721 ttttgcaata gaaattttag attatacggc aaaaatgttg ggcgaggcac tagctgattt
      781 catgtgtttt tctaatccag aagcttttgt tttatttgga ggaatggcaa aagtgggtca
      841 agcgtttgct caacaagtag aagcctatat gaatgctgct gctttgaaaa tttatcaaga
      901 taaaaccaaa gtagtaattt caaccataca agaccataat gcagctattt taggcgcagc
      961 ggctcttgtt ttccatcaag attaattaaa gtagcgtttt attgtaaaat tatttgatgc
     1021 atcaacccgt aataggcttc gtttcactgc agaattttca aaaaaaataa aaagtgttac
     1081 caacttccct ccttgaggag ggaaagaggg aggtgata
//
LOCUS       DAOQVQ010000618         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_191416_length_1115_cov_4.597170, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000618 DAOQVQ010000000
VERSION     DAOQVQ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191416_length_1115_cov_4.597170"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PKN22_12470"
     CDS             <1..862
                     /locus_tag="PKN22_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS43564.1"
                     /translation="TATTQVVTPSQTTVYTVEVTDICDLTETYDVTYTVTSPPLVVTL
                     NPSVEVCPGDSVQLVATVTGGYGQYYYHWPHDGATTPSTWANPYVTTDYMVVVSDECQ
                     TFSVAEKVRIYVVKPTANFAMISNVPFNNIDLTFQNLSSNASTYEWTFGDGDSSTVVH
                     PTHLYDNPGNYTVTLIATDDKGCKDTIAKTITILEEYYIYVPNSFTPNDNRINDGFMA
                     STVNIESLNVTIYDRWGERIFKSDHVNFIWDGTYRGALMQDGTYTYKINYVTRRGIEG
                     KLVGHVNLLR"
     gene            complement(859..>1115)
                     /locus_tag="PKN22_12475"
     CDS             complement(859..>1115)
                     /locus_tag="PKN22_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43565.1"
                     /translation="LLEHLTCLTDQPVEVLDQIAGIRPTTVDRRPLMGLHPEFNKLAV
                     FNGLGAKGFLLAPLLSKEMCAYLLEGKDLDKECVLQRIKA"
BASE COUNT          343 a          233 c          212 g          327 t
ORIGIN      
        1 cacagcaacc actcaagttg tgacgccttc acaaacaact gtttataccg ttgaagtaac
       61 agatatttgt gatttaacag aaacgtatga tgttacctat accgtaacca gcccaccttt
      121 ggttgttacg ctcaacccaa gcgttgaagt ttgtcctggt gactccgttc aattagtagc
      181 aaccgttaca ggtggatacg gacaatatta ttaccattgg ccacacgatg gagcaacaac
      241 tccaagcaca tgggcaaatc cttatgttac taccgattac atggtagtag tttccgatga
      301 atgccaaacg tttagtgtgg ctgaaaaagt gcgcatttac gttgtaaaac caacggccaa
      361 ttttgcaatg attagcaatg tgccgtttaa caatatcgat ttaacatttc aaaacttaag
      421 ttccaacgct tcaacctatg agtggacctt tggcgatggt gattcatcaa ctgttgtgca
      481 ccctacgcat ttgtatgaca atcctggaaa ttacacggtt actttaattg ctacagacga
      541 taaaggttgt aaagacacca ttgcaaaaac cattaccatt ttagaagagt attatattta
      601 cgtgcccaac tcatttacac caaatgacaa ccgtatcaat gatgggttta tggcttcaac
      661 tgtaaatatt gaatcgttaa acgtaacaat ttatgaccgt tggggtgaac gtatttttaa
      721 atcagaccat gtgaacttta tctgggatgg aacctatcgc ggtgcattga tgcaagacgg
      781 aacttatacc tataaaatca attacgtcac ccgaagggga attgaaggta agttggttgg
      841 gcacgtcaat ttgttgcgtt aagccttaat gcgttgcaac acacattctt tgtccaaatc
      901 ttttccttcc aacaaataag cacacatttc tttggataaa agcggagcta ataaaaatcc
      961 ttttgctccc aacccattga atacagccaa tttattgaat tctgggtgca atcccattaa
     1021 aggacgacgg tcaacagttg ttggtcgaat accagctatt tgatcgagca cttctactgg
     1081 ttgatcggtt aaacaagtca agtgttccaa caatt
//
LOCUS       DAOQVQ010000619         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_192105_length_1113_cov_2.550095, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000619 DAOQVQ010000000
VERSION     DAOQVQ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192105_length_1113_cov_2.550095"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKN22_12480"
     CDS             complement(<1..511)
                     /locus_tag="PKN22_12480"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNS43566.1"
                     /translation="MAKIIPFKAIRSVRDKVHLVATRPIYSYKKNVLESKLEDNPYTF
                     LHIINPEFGGERTTQPNTPERFELVKKEYKKFIDEGILFQDASPRIYIYQQTKNGHAF
                     KGVIAGASNQEYREDAIKKHEATLTSREEMFIDYLDIVGLNAEPVLLSYKHNNLIDNK
                     INEIIAQRPE"
     gene            complement(654..>1113)
                     /locus_tag="PKN22_12485"
     CDS             complement(654..>1113)
                     /locus_tag="PKN22_12485"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNS43567.1"
                     /translation="HPTRSVEDKFKQFLGEKWGYLYPKFEWLGLFDGEKTLKIPSPTP
                     AKLLQEILERKWTLNTGDKDMLVMIHEFEYILNNQTHKITSSMVNIGEDQVYTSMSNT
                     VGLPVGMGAKLILNNQITEKGVLLPIKKEIYEPILEELALYGIEFSEKHH"
BASE COUNT          311 a          188 c          196 g          418 t
ORIGIN      
        1 attctggtcg ttgcgctata atttcgttta ttttgttgtc tatcaaatta ttgtgtttgt
       61 aggatagcaa cacgggttct gcattcaaac caacaatatc caagtaatcg ataaacattt
      121 cttcgcgact ggttaacgtt gcttcgtgtt tttttatagc atcctcgcgg tattcttgat
      181 tgcttgctcc ggcaataact cctttgaatg catgaccgtt tttggtttgc tggtagatgt
      241 atatgcgggg agaggcatct tggaataaaa taccttcgtc aatgaatttt ttatattctt
      301 tttttaccaa ctcaaaacgt tcgggcgtat tgggttgcgt agttctttct cctccaaact
      361 ctggattaat gatgtgtaga aaagtgtagg gattatcttc caattttgat tccaatacgt
      421 tttttttgta cgaataaatt ggtcgagtag caacaagatg aactttgtcg cgaacagatc
      481 gaatagcttt gaatggaata attttagcca ttgttaatac gtaatttgat acactaattt
      541 tgtggtttca aatctaatct ttttcttgta tttagcaatg aaaatcgtgt ttaattacga
      601 aaatgttaca ccttgttaga aaacaaactt taaatggatt ttttttactt tagttaatgg
      661 tgtttttcgc taaattcaat accataaaga gctaattctt ccaaaattgg ttcataaatt
      721 tcctttttga taggtaaaag cactcctttt tcggtaattt gattgttgag aattaattta
      781 gctcccattc caacaggcaa tccaacggta ttggacatag aagtgtacac ttgatcttcg
      841 ccaatgttaa ccatactcga agtgatttta tgggtttggt tgttaagtat gtattcaaat
      901 tcatgaatca ttaccaacat atctttatca cctgtattga gggtccattt gcgttccaat
      961 atttcttgca acaatttagc aggtgttggc gaagggattt tcaatgtttt ttcaccatca
     1021 aacaaaccta accattcaaa tttagggtac aaataccccc atttttcacc taaaaactgt
     1081 ttgaatttat cttctaccga acgagttggg tgg
//
LOCUS       DAOQVQ010000620         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_192994_length_1110_cov_2.703318, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000620 DAOQVQ010000000
VERSION     DAOQVQ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192994_length_1110_cov_2.703318"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKN22_12490"
     CDS             complement(<1..757)
                     /locus_tag="PKN22_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNS43568.1"
                     /translation="MKSSINLNNTEKAFAYKSDAELKNTIFIFGLLQKPFLVKMLMKI
                     ADWIITFKLPLKFVLRKTVFQVFCAGENRSEAAKTIQHIKKYNVNTVLDYVAEGDNSE
                     ASFENNLQTILDNIQFVSKNAADATVGVKLSGLEDVAFLKQFKTIDEGNSDIAILRLA
                     KFTERVEKICQLGSDLHVKIYIDAEEYSTQFIFDNVVELMMEKFNKKEVIVYNTLQMY
                     LTDRVDYLNKAIAEARAKGYLLGMKLVRGAYVEK"
     gene            complement(754..>1110)
                     /locus_tag="PKN22_12495"
     CDS             complement(754..>1110)
                     /locus_tag="PKN22_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43569.1"
                     /translation="AHSMGTTIGINTMILFAAVFMFFTPKTAHEGKPSIVLRSTFWVI
                     QIALLCLFLSLKTAGLLKGFWQLDENQVSFYDMMQSLQPLFIVFVTSGTILMIAFFYL
                     LGTIFKYSRINKKLNA"
BASE COUNT          349 a          221 c          168 g          372 t
ORIGIN      
        1 ctttttcaac atacgcacca cgcaccaatt tcattcctaa taaataaccc tttgcacgtg
       61 cttcggcaat ggctttgttc aaataatcca ctctgtcggt tagatacatc tgcaacgtgt
      121 tgtaaacaat tacctctttt ttattgaatt tctccatcat taattccacc acattatcga
      181 aaatgaattg cgtagaatat tcttcggcat caatgtagat ttttacgtgt aaatcgcttc
      241 ctaattggca aattttctcc actctttcgg taaatttagc taatcgcaat attgcgatat
      301 cactatttcc ttcgtcgatg gttttaaatt gttttaaaaa cgcaacatct tctaatccag
      361 atagttttac accaaccgtt gcatccgcag cattcttgct cacaaattga atgttgtcta
      421 aaattgtttg caaattattc tcaaaactag cttccgaatt atccccctca gcaacgtaat
      481 ccaaaaccgt atttacattg tattttttaa tgtgttggat agtttttgcc gcttccgatc
      541 tgttctcacc tgcacaaaaa acttgaaaca ccgtttttct caaaacaaat ttcaacggca
      601 atttgaatgt gatgatccaa tcggcaattt tcatgagcat tttaaccaaa aatggctttt
      661 gcaacaaccc aaaaataaag atagtgtttt tcaattcagc atccgattta taagcaaatg
      721 ctttttcggt gttatttaga ttgatggatg atttcatgcg tttaattttt tattgattct
      781 actgtattta aaaatggttc caagtaaata aaagaatgca atcattaaaa ttgttcccga
      841 tgttacaaac acgataaaca ggggttgcag actttgcatc atatcgtaaa aagaaacttg
      901 attttcgtct aattgccaaa atcctttcaa taatccagca gttttaagcg ataagaacag
      961 gcacaaaagc gcaatttgaa taacccaaaa agtgctgcga agaacgatgc ttggtttacc
     1021 ttcgtgcgcc gtttttggag taaaaaacat aaaaacagct gcaaaaagta tcatggtgtt
     1081 gatgccaata gttgttccca tggaatgagc
//
LOCUS       DAOQVQ010000621         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_194485_length_1105_cov_2.656190, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000621 DAOQVQ010000000
VERSION     DAOQVQ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194485_length_1105_cov_2.656190"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1105
                     /locus_tag="PKN22_12500"
     CDS             <1..>1105
                     /locus_tag="PKN22_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNS43570.1"
                     /translation="HTMQFHPHGDASIGDALVQLGQKDLLIDCQGNWGNTLTGDGAAA
                     PRYIEARLTKFANHVVFNPKTTNWLKSYDGRNDEPEQLPVKFPLLLAQGAEGIAVGMA
                     CKILPHNFIELIDQSINHLRGKRVEIFPDFPNGGMADFSNYNDGLRGGKVRLRAKIKK
                     EDSKTLIINEIPVGTTTSLIDSILKANDKGKIKIKKIEDNTAANVEIIIHLAPGVSPD
                     KTIDALYAFTDCEVSISPNASVIDGDKPRFIGVSEILTINTNNTVDLLRKELEIRLDE
                     LQRQWHFSTLEKIFINNEMYIDFKHYSDKESLFAYLYKSFEPFKKQLIREIVDEDLQK
                     LTQIPMIRITRFDASKADDLIAKIEAEMEEIKNN"
BASE COUNT          376 a          200 c          216 g          313 t
ORIGIN      
        1 gcacaccatg caatttcacc cacatggcga cgcttctatt ggcgatgcgt tggtgcaatt
       61 gggtcaaaaa gacttgttaa ttgattgcca aggaaactgg ggaaacacat taactggtga
      121 cggtgctgca gctccacgtt atattgaagc acgtttaaca aaatttgcta atcacgttgt
      181 ttttaatcct aaaactacca attggctaaa aagctatgac gggagaaacg atgaacccga
      241 acaattacct gttaaattcc ccttgctttt ggctcaaggt gccgaaggaa ttgctgttgg
      301 gatggcgtgt aaaattttac cacacaattt tatagaacta attgaccaat ccatcaacca
      361 tttaagagga aaacgcgttg agatttttcc agattttccc aatggtggta tggccgattt
      421 ttctaattac aacgatgggt tgagaggagg aaaagttaga cttagagcaa agattaaaaa
      481 ggaagactct aagacactga taataaatga gattccagtc ggaacaacta cctcgttaat
      541 tgattctatt ctaaaggcaa acgacaaagg aaagataaaa atcaaaaaaa ttgaggacaa
      601 cacggctgct aatgttgaaa tcattattca ccttgctcca ggcgtatctc ccgataaaac
      661 catagatgcg ctgtatgctt ttacagattg tgaagtttct atttctccta atgcttcggt
      721 aattgatggc gacaaaccac gttttattgg tgtgagcgaa atattgacaa tcaacaccaa
      781 caacacggtt gatttattgc gtaaggaatt ggaaatacgc ctagatgaat tacaacgtca
      841 atggcatttc tcaaccttgg aaaagatatt catcaacaat gaaatgtaca ttgatttcaa
      901 gcattattct gacaaagaat cgttgtttgc ttatttgtac aaatcgtttg agccgttcaa
      961 aaaacaattg attcgcgaaa ttgtagatga agatttgcaa aaactaacgc aaattcctat
     1021 gattcgtatt acccgtttcg atgcttcaaa agcagatgat ttgattgcta aaattgaagc
     1081 cgaaatggaa gaaatcaaaa acaat
//
LOCUS       DAOQVQ010000622         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_197227_length_1096_cov_3.044188, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000622 DAOQVQ010000000
VERSION     DAOQVQ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197227_length_1096_cov_3.044188"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..152)
                     /locus_tag="PKN22_12505"
     tRNA            complement(79..152)
                     /locus_tag="PKN22_12505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Met,seq:cat)
     gene            complement(262..1032)
                     /locus_tag="PKN22_12510"
     CDS             complement(262..1032)
                     /locus_tag="PKN22_12510"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNS43571.1"
                     /translation="MKTKEEIVNNWLPRYTGEEIENFGKYILLCNFSFYIETFAKEFN
                     AEIKGVGGPMQCATANGITIINFGIGSASAATCMDLLSAIHPKAVLFLGKCGGLKKKC
                     EVGDLILPIAAIRGEGASLDYFPSEVPALPAFALQKAISTTIRDFGRDYWTGTCYTTN
                     RRIWEHDEEFKEYLRKIRALAIDMETATIFSVGFFNEIPTGALLLVSDQPMIPEGVKT
                     SESDKKVTSNFAVEHIKIGIESLKQLINNGHTVKHLKF"
BASE COUNT          313 a          227 c          182 g          374 t
ORIGIN      
        1 aaagctattt tttaaaacat aaaaaatggc cggaatttct tccgaccatt cgtgctcaat
       61 tgagcgacct tttttttgta gcgagaccga gagttgaact cgggacctca gggttatgaa
      121 tcctgcgctc taaccaactg agctatctcg ccaaggttat tgcgggtgca aaaatagtga
      181 ttcttttcat ttttccaact atccaaccac ttttttttct gcttttagct gaattttctt
      241 ttcacctgca aaaaagtaga actaaaattt caaatgtttt acggtatgtc cgttattgat
      301 taactgttta agcgattcta taccaatttt tatatgttct accgcaaaat tggaggttac
      361 ttttttatcg ctttccgatg tttttacacc ttcgggaatc attggttgat cagaaaccaa
      421 taacaacgct ccagttggaa tctcattaaa aaagcctacg ctaaaaatcg ttgccgtttc
      481 catatcaatt gcaagtgcgc gtatttttcg gaggtattct ttaaactctt catcgtgttc
      541 ccaaattctg cggttggtgg tatagcacgt tcctgtccaa taatctctac caaaatcgcg
      601 aatggttgta gaaattgcct tttgcaaagc aaacgctggc aatgctggta cttcagaagg
      661 aaaataatcc aaacttgctc cttcaccacg aatggctgca atgggtaaaa ttaaatcacc
      721 gacttcacat tttttcttga gtcccccaca ttttcctaaa aacaacactg ctttaggatg
      781 aatggctgac aataaatcca tgcacgttgc tgccgaggca ctccctattc caaaatttat
      841 gatggtaatt ccgttagccg tggcacattg cattgggcct cctacacctt taatctctgc
      901 attaaattct ttggcaaatg tttcaatgta aaacgaaaaa ttacatagta aaatgtattt
      961 tccaaaattt tcaatttctt cacccgtata gcgcggtaac cagttgttta caatttcttc
     1021 ttttgttttc atagtgttta attgtagtgt aagatagtaa aaaaattaat tctttgccaa
     1081 aagatgtatc ttctct
//
LOCUS       DAOQVQ010000623         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_203173_length_1077_cov_2.426614, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000623 DAOQVQ010000000
VERSION     DAOQVQ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203173_length_1077_cov_2.426614"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKN22_12515"
     CDS             complement(<1..272)
                     /locus_tag="PKN22_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43572.1"
                     /translation="MKSVAKITFSLIVLSSLIFTSCRKKELPAPKPNTGDVITTTVNM
                     HSNYDRIIYFNLGNNKVVSETNKLTWDLAFSCNEGTQYGIMNGAKI"
     gene            complement(272..>1077)
                     /locus_tag="PKN22_12520"
     CDS             complement(272..>1077)
                     /locus_tag="PKN22_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS43573.1"
                     /translation="KSPVVPSLFIRYEINKNNSIRASYSRGFRAPSIKELFFYFVDIN
                     HNIVGNENLKAETSNNYQLSYRADYQIKKTRFSINNQFFFNDLHNLITLAQVNNVEYS
                     YVNLFKAQTYGYTLNANILYRELSIDAGLSIKARKNQIVETQAPRATSFYPEIKFNPS
                     YTVNKINTTFAFFYKFTGKLPNLMLQTDGTVIEAKREAYHMLDFTISTHFLKRMIGLT
                     IGAKNLLNVTSINGMTGSGGAHSSGGSSVLIGMGRTYFCSLTINFNTKK"
BASE COUNT          341 a          156 c          197 g          383 t
ORIGIN      
        1 ataatttttg caccattcat aattccgtat tgcgtacctt cattgcaaga gaatgctaag
       61 tcccaagtta atttattggt ttcgctcacc actttgttgt tacctaaatt aaagtaaata
      121 atacgatcgt aattagagtg catgtttaca gtagtggtga ttacatcacc tgtattgggt
      181 tttggagcag gtaattcctt ttttctacac gaagtaaaaa ttaagctact taatacaatt
      241 aaactgaaag ttattttagc aacagatttc attatttctt agtattaaaa ttaatagtta
      301 acgaacagaa gtatgttctt cccattccaa taagtacgct cgatccacca gatgaatgtg
      361 cgccaccaga accagtcatt ccgttgatag atgtaacgtt taataagttt ttagcaccga
      421 tagtcaaacc aatcattcgt tttaagaaat gagttgaaat ggtaaagtcc aacatgtggt
      481 atgcttcgcg tttagcttca atcactgttc cgtctgtttg caacattaag ttaggtaatt
      541 ttccagtaaa tttatagaaa aacgcaaaag tagtgttgat tttgttgaca gtataacttg
      601 ggttgaattt aatttctgga taaaatgaag tagcacgagg tgcttgtgtt tcaacgattt
      661 gattttttct agctttgatt gataaacctg catcaattga aagttcacgg tataaaatat
      721 tagcatttaa tgtgtaaccg taagtttggg ctttgaataa gtttacatag ctgtattcca
      781 cattatttac ttgtgctaag gtgattaaat tgtgtaaatc gttaaaaaag aattggttat
      841 tgatagagaa acgtgtcttt tttatttgat aatcagctct gtaggataac tggtagttgt
      901 tagaagtttc tgcttttaaa ttttcgttac caacgatgtt gtggttgata tctacaaagt
      961 agaaaaacaa ttctttgata gaaggagctc taaatccgcg actgtaggat gcacgaatac
     1021 tgttgttttt attgatttca tagcgaataa aaagtgaagg aacaacagga cttttat
//
LOCUS       DAOQVQ010000624         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_204647_length_1072_cov_3.500492, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000624 DAOQVQ010000000
VERSION     DAOQVQ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204647_length_1072_cov_3.500492"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1072
                     /gene="ribD"
                     /locus_tag="PKN22_12525"
     CDS             37..>1072
                     /gene="ribD"
                     /locus_tag="PKN22_12525"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNS43574.1"
                     /translation="MTDEFYIKRCIELALKGKGNVAPNPMVGAVIVHNNQIIGEGYHQ
                     HYGEAHAEVNAVNSVEDKTLLKEATIYVSLEPCAHHGKTPPCADLLVQCGFKRVVIGC
                     VDTFSQVAGKGIERLQQAGIDVTVGVLEKACRELNKRFFTFHEQKRPYVILKWAQTQD
                     GFIDKKRTNAEKGINWITGATTKKIVHHWRAEEAAILVGKNTVLNDNPSLTVREVEGK
                     NPIRIVLDSQMELTTQFAVFDGTATTFILNKVGNKVEGKNEWIQLDSLTPEAILNALY
                     NRNIQSVIIEGGKQTLNAFINANLWDEARVLTGNVEWKEGVFAPRLPHQYFVEEFQLD
                     QDKVAIYRYFN"
BASE COUNT          351 a          184 c          233 g          304 t
ORIGIN      
        1 tggataccat gcaatggctc aatcaacttt aaacaattga cagacgaatt ttacataaaa
       61 cgatgcattg aactggcgct aaaaggcaaa ggaaatgttg cccccaatcc gatggtggga
      121 gctgttattg tgcacaataa tcaaatcatt ggcgagggct atcatcaaca ctacggagaa
      181 gcacatgccg aggtgaatgc tgtcaattcg gttgaagata aaaccttgtt gaaagaagca
      241 actatttatg tttcattgga gccgtgtgca catcatggaa aaacacctcc gtgcgcagat
      301 ttgttggtgc aatgtggttt taaacgtgtt gttatcggtt gtgttgatac tttttctcaa
      361 gttgcgggta aaggtattga acgtttgcaa caagctggca ttgacgttac tgttggtgtg
      421 cttgaaaaag cttgccgtga gctcaataaa cgttttttta cttttcacga acaaaaaaga
      481 ccctatgtaa ttctaaaatg ggcacaaaca caagacggtt ttattgataa aaagcgcacc
      541 aatgctgaaa agggaatcaa ttggataaca ggagcaacaa ctaaaaaaat tgtgcaccat
      601 tggcgagcag aagaagcggc aattttggtt ggaaaaaata ctgttttaaa cgataatcca
      661 tcgttaaccg tgcgagaagt cgaaggtaaa aatcctatac gcattgtgtt ggatagccaa
      721 atggaactga ccacacaatt tgctgttttt gatggtactg ctacaacatt cattctcaat
      781 aaagttggca ataaagtgga aggtaaaaat gaatggattc aactcgattc tctaacaccc
      841 gaagcaattt tgaacgcatt gtataatcgt aatattcaat cggttatcat cgaaggtggt
      901 aaacaaacct tgaatgcttt tatcaatgcc aatttgtggg atgaagctcg cgttttaact
      961 ggaaatgtgg aatggaaaga aggtgttttt gccccacgtt tgccgcatca atattttgta
     1021 gaagaatttc aattagacca agataaagtg gcaatttatc gctactttaa ta
//
LOCUS       DAOQVQ010000625         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_207857_length_1062_cov_3.636544, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000625 DAOQVQ010000000
VERSION     DAOQVQ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207857_length_1062_cov_3.636544"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKN22_12530"
     CDS             <1..220
                     /locus_tag="PKN22_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43575.1"
                     /translation="GVWTYYTVDGKKQSVTEYKHGIKDGFSIVYHPNGSVYYKGEYRN
                     DQQVGVWDFYDTNTGKKAYSKDYGQPKV"
     gene            228..>1062
                     /locus_tag="PKN22_12535"
     CDS             228..>1062
                     /locus_tag="PKN22_12535"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNS43576.1"
                     /translation="MKTKTLKKNKVNVVTLGCSKNVYDSEVMMAQLRANKFDVEHESK
                     QDDAEIVIINTCGFIDVAKQESIDTIIRYAEAKADGMVDKLYVTGCLSERYKNDLEKE
                     IPEVDAYFGTRELPRLLKTLKADYKHELVGERLLTTPNHYAYFKISEGCDRNCSFCAI
                     PIMRGGHISVSIEELVKQAKSLANQGVKELMLIAQDLTFYGLDIYKKRALADLLEQLA
                     QVEGIEWIRLHYAFPSGFPMDVIDVMNKYDNICNYLDMPLQHGSTKMLQAMRRGTTRE
                     KT"
BASE COUNT          360 a          171 c          229 g          302 t
ORIGIN      
        1 tggtgtgtgg acctattaca ctgttgatgg taaaaaacaa tctgttaccg aatacaaaca
       61 cggcattaaa gatggttttt caatcgtgta tcatcccaac ggttcggtgt attacaaagg
      121 tgagtaccgc aacgatcaac aagtaggtgt gtgggatttt tacgatacca acactgggaa
      181 aaaagcatat tcaaaagatt acggtcaacc aaaagtttaa agcagcaatg aaaacaaaaa
      241 cattaaaaaa gaataaagta aatgttgtaa cccttggttg ctcaaaaaat gtgtacgact
      301 ctgaggtgat gatggcgcaa ttacgtgcca acaaatttga tgtggaacac gagtcaaaac
      361 aagatgatgc agagattgta attatcaaca cctgtggttt tattgatgtt gcaaagcaag
      421 aatctattga taccatcatt cgctatgctg aagctaaagc agatggaatg gtggataaat
      481 tatatgttac aggttgtttg tctgaacgtt ataaaaatga tttggagaaa gaaattccag
      541 aagtagatgc ttatttcggt acgcgtgaat tgcctcgttt gcttaaaacg ttaaaagcag
      601 attacaaaca cgaattggta ggtgaacgtt tattgactac gcccaaccat tacgcctatt
      661 ttaaaatttc ggaaggttgt gaccgtaatt gttcgttttg tgccattcca attatgcgtg
      721 gtggccacat ttctgtttcc attgaagaat tagtgaagca agcaaaaagt ttggcaaatc
      781 aaggcgtgaa agaattaatg ttgattgcac aagacttaac tttctatgga ttagacatct
      841 ataaaaaacg tgcattagca gatttattgg agcaattggc gcaagtggaa ggaattgaat
      901 ggattcgttt gcactatgcg tttccgtctg gtttccctat ggatgtaatt gatgtaatga
      961 acaaatacga caacatatgc aactatttag atatgccgtt acaacacggt tctaccaaaa
     1021 tgttgcaagc gatgcgtaga ggaacaactc gcgaaaaaac cg
//
LOCUS       DAOQVQ010000626         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_208159_length_1061_cov_4.569583, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000626 DAOQVQ010000000
VERSION     DAOQVQ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208159_length_1061_cov_4.569583"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..811
                     /locus_tag="PKN22_12540"
     CDS             269..811
                     /locus_tag="PKN22_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43577.1"
                     /translation="MNWEDIIFYVALITLPAGAVLMTTILFLRKQTERELNSQFHNVQ
                     SELKKQRQEYFLPNRVEAYQRAVLLMERIHPNSLIMRTINPALPSAVYQTELLKTIRE
                     EYDHNVAQQLFVSPAMWEMVKNSKEETIKIINIAGQQMSVTSMSTDLAAKIFELVAEI
                     GELPSEITVKALKEDLQKLF"
     gene            822..>1061
                     /locus_tag="PKN22_12545"
     CDS             822..>1061
                     /locus_tag="PKN22_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6122 family protein"
                     /protein_id="HNS43578.1"
                     /translation="MESIYWSTFQNCVHYSLHFIVPIFVARHFFKAQWLKVTLVLWAT
                     MLVDLDHLWATPLFDPNRCSIDFHPLHSYFAILAYA"
BASE COUNT          341 a          183 c          199 g          338 t
ORIGIN      
        1 tgacgttcct atcattccaa atgctactaa ataccattta ttttggcggt gaccagtgaa
       61 aaatgtttcg gtatctgctc cttttgacgt gaaataggaa atcaaaatca aaataagaaa
      121 gtatatggat aaaatcgaaa cgatataagt tggtgtcata aatttctttt tgggggtgaa
      181 aatacaaaaa tcattgtagt tttaaattga attgaatgct atttcaatag aaaattctat
      241 tttcgttgca gaaaaacaaa tgacggatat gaattgggaa gatattattt tttatgtagc
      301 gttgattact ttaccagctg gagcggtgtt gatgacaact attctttttt taagaaaaca
      361 aacagaacgc gagttgaaca gtcagtttca caacgtgcaa agcgaattaa aaaaacaacg
      421 acaagaatat tttttaccca atcgtgtaga agcttaccaa cgtgcagttt tgttaatgga
      481 acgcattcat cccaacagtt tgattatgcg caccatcaat ccggcgttgc cttcggctgt
      541 ttaccaaacc gagttgttaa aaaccatccg agaagaatac gatcacaacg ttgcacaaca
      601 attatttgta tcccctgcga tgtgggaaat ggtaaaaaat tcgaaagaag aaaccataaa
      661 aatcatcaat attgctgggc aacaaatgag cgttacttcc atgagtacgg atttggctgc
      721 taaaatcttt gaattagttg ccgaaattgg tgagttgcct tcagaaatta ccgtaaaagc
      781 attaaaagaa gacttgcaaa agttgttcta actgttcgta aatggagtcg atttattggt
      841 ctacatttca aaattgcgtc cattactcgt tgcattttat tgttccaatt tttgttgcgc
      901 ggcatttttt taaagctcaa tggctaaaag ttaccctagt tttgtgggca acaatgttgg
      961 tagatttgga tcatttatgg gcaacaccac tttttgaccc caatcggtgc agcatagatt
     1021 ttcatccact ccactcctat tttgctattt tggcttatgc c
//
LOCUS       DAOQVQ010000627         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_209820_length_1056_cov_4.381618, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000627 DAOQVQ010000000
VERSION     DAOQVQ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209820_length_1056_cov_4.381618"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKN22_12550"
     CDS             complement(<1..435)
                     /locus_tag="PKN22_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43579.1"
                     /translation="MLNRRHLRIKILQSLYAYFQSEDDKYPQAEKALYGAIDKIYHLY
                     IYLMLSIPEVKYFAERRIDENKKKIRPTKEELNPNLKFVNNSIIQKIETDKKLIQVSE
                     LNKINWIGDEKQELFRKMFLAIRESETYFEHMNNGNTGFEADK"
     gene            complement(449..>1056)
                     /locus_tag="PKN22_12555"
     CDS             complement(449..>1056)
                     /locus_tag="PKN22_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HNS43580.1"
                     /translation="VYVGMKACAKEQFGSDSLEGKKISVQGVGHVGEYLVEFLAKEKA
                     EIYITDVYEPTLKRVAEKYGAKVVGLDEIYDLDVDIYAPCALGATVNDETLNRLKCSI
                     IAGSANNQLKDEARHGKMVMEKGIIYAPDYAINSGGVINVYSEFKGLSSEWAMQNAEN
                     IYSTIAQIVKRSKDENIPTYKIANKMAEERIESVGKIKLPR"
BASE COUNT          302 a          216 c          148 g          390 t
ORIGIN      
        1 tttatccgct tcaaaacctg tattgccatt gttcatgtgc tcaaaatagg tttcactttc
       61 gcggatagct aaaaacatct tgcgaaacaa ttcctgtttt tcatcaccga tccaattgat
      121 tttgttcaac tcagaaactt gaatcaactt tttatccgtt tcaatttttt ggataattga
      181 attgtttaca aatttcagat tgggattcaa ctcctctttg gttgggcgaa ttttcttttt
      241 attctcgtca attcttcttt cggcaaagta tttaacctct ggaattgaca acattaaata
      301 gatgtaaagg tgatagattt tatcaattgc accatacaac gccttttcgg cctgaggata
      361 tttgtcatcc tcagactgaa aatacgcata caatgactgt aaaattttaa ttcgaagatg
      421 tcttctattt aacattctat tgttttaatt atctcggtaa tttaattttc ccaacgcttt
      481 caatacgctc ttctgccatt ttgttagcaa ttttatacgt aggaatgttt tcgtcttttg
      541 aacgtttcac gatttgcgca attgttgaat aaatattttc tgcattttgc attgcccact
      601 ctgatgaaag tcctttaaat tctgaataaa cattaattac acctcctgag ttaatggcat
      661 aatctggagc ataaataatt cctttttcca ttaccatttt accgtgacgt gcttcatctt
      721 tcaactggtt gtttgcagaa ccagcgatga tagaacattt taaacggttc aatgtttcat
      781 cgtttacagt tgcaccaagt gcacaaggtg catagatatc aacgtctaag tcgtaaattt
      841 catccaatcc aacaacttta gcaccgtatt tttctgccac gcgtttcaac gttggttcgt
      901 acacatcggt aatataaatt tcagcttttt cttttgctaa aaactcaacc aaatattcac
      961 ctacgtgtcc aacaccttga acagaaatct ttttaccttc taatgaatct gaaccaaatt
     1021 gttctttagc acaagctttc attccaacat aaacac
//
LOCUS       DAOQVQ010000628         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_212622_length_1048_cov_2.716012, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000628 DAOQVQ010000000
VERSION     DAOQVQ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212622_length_1048_cov_2.716012"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKN22_12560"
     CDS             <1..466
                     /locus_tag="PKN22_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNS43581.1"
                     /translation="IKNVKYAIENYRVEQKTDYEKLVFDIITDGSIHPKEALKEAAKI
                     LIHHFLLFSDERITLDSEIKSETEEFDETSLHMRQLLKSKLVDMDLSVRALNCLKAAD
                     VETLGDLVTYNKNDLLKFRNFGKKSLTELEDLVDNKGLTFGMNVSKYKLDKD"
     gene            563..>1048
                     /gene="rplQ"
                     /locus_tag="PKN22_12565"
     CDS             563..>1048
                     /gene="rplQ"
                     /locus_tag="PKN22_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNS43582.1"
                     /translation="MRHGKKINHLGRTASHRAAMMSNLASSLILHKRINTTVAKAKAL
                     RSYVEPLLTKSKEDTTHNRRTVFSYLQNKEVVAELFRTVAPKIADRPGGYTRIIRTGF
                     RLGDNAEMCMMELVDFNEIYTKDAKKTSRRSRRGGGSKATTETVATEEVVETPVVEET
                     PA"
BASE COUNT          378 a          159 c          197 g          314 t
ORIGIN      
        1 aattaaaaac gttaaatacg ctatcgaaaa ctatcgtgtt gagcaaaaaa cagattatga
       61 aaaattagtt tttgatatca tcactgatgg ttcaattcat ccaaaagaag ctttgaaaga
      121 agctgctaaa attttaattc accatttctt gttgttctca gatgaaagaa ttacgttgga
      181 tagcgaaatt aaatctgaaa cagaagaatt tgatgaaact tcattgcaca tgcgccaatt
      241 gttaaaatct aaattggtag atatggactt gtctgttcgt gcattgaact gcttgaaagc
      301 tgctgatgtt gaaactttag gtgacttggt tacttacaat aaaaatgatt tattgaagtt
      361 ccgtaacttc ggtaagaaat cgcttactga gttggaagac ctagttgata acaaaggttt
      421 gacttttgga atgaatgttt ctaaatacaa attagataaa gactagtatc gcaaataaaa
      481 taatgttaaa tagcgtaata ggtggtaagg aaacgcgata ctaaaaattc ttactaaccg
      541 ttacttacga aattaaatta aaatgagaca cggaaaaaaa atcaatcatt taggaagaac
      601 tgcttctcac agagcagcaa tgatgtctaa cttggcttct tctttaattt tgcacaaacg
      661 catcaataca actgttgcaa aagctaaagc tttgagatct tatgtagaac ctcttttgac
      721 taaatcaaaa gaagatacaa ctcacaacag acgtactgtt tttagttact tgcaaaataa
      781 agaagtggtt gctgaattgt tcagaactgt tgcacctaag attgcagacc gtccaggagg
      841 atatactcgt attatccgta ctggtttcag attgggtgac aatgcagaaa tgtgtatgat
      901 ggaattagtt gacttcaatg aaatctacac aaaagatgct aagaaaactt ctcgtcgttc
      961 aagaagaggt ggtggttcta aagctacaac tgaaactgtt gcaactgaag aagttgttga
     1021 aacaccagtt gttgaagaaa ctccagct
//
LOCUS       DAOQVQ010000629         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_216465_length_1037_cov_3.591650, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000629 DAOQVQ010000000
VERSION     DAOQVQ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216465_length_1037_cov_3.591650"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKN22_12570"
     CDS             complement(<1..678)
                     /locus_tag="PKN22_12570"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNS43583.1"
                     /translation="MNTYLDFEQPIKELEEQIASTKEIGENTGVDMSDKIKELEKKLI
                     KETKAIFSGLTPWQRVQLSRHPDRPYSLAYIDYITNGTFQELHGDRGVRDDKAMVGGF
                     GLVDGQSVMFIGQQKGINTKMRQYRNFGMPNPEGYRKALRLMKLAEKFNKPIVTFIDT
                     PGAFPGLEAEERGQGEAIARNIYEMMRLKVPVLCIIIGEGASGGALGIGVGDKVTMLE
                     NTWYSVIS"
     gene            785..>1037
                     /locus_tag="PKN22_12575"
     CDS             785..>1037
                     /locus_tag="PKN22_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43584.1"
                     /translation="MSTQKRIAIVEIGGSHDECILSQLIGLKQAGAWVALCCDKKMYE
                     KNSAFKNTIDCFHEVSFPHTMLGDFFTMKQLNKWFKEQNI"
BASE COUNT          333 a          208 c          163 g          333 t
ORIGIN      
        1 agaaattaca gaataccacg tattttccaa catggttacc ttatctccta ctccaattcc
       61 caacgctcca ccagaagcac cttctccaat aatgatacaa agcacaggga cttttaaacg
      121 catcatttcg tagatgttgc gtgcaatcgc ttctccttga ccacgttctt cagcctccaa
      181 acctggaaaa gctcccggag tatcaataaa cgttacaatt ggtttgttga atttttctgc
      241 caatttcatc aaacgtaagg cttttctata accttctgga ttgggcatac caaaattacg
      301 gtactggcgc attttagtgt taattccttt ttgctgacca ataaacataa ccgattgacc
      361 gtcaacaagt ccaaaaccac ccaccattgc tttatcatct cttacacctc tatctccatg
      421 caattcttga aaagttccgt tagtgatgta atcaatgtat gccaatgaat aaggtctatc
      481 tggatgacgc gacaattgta cacgttgcca aggtgttaaa cctgagaaaa tagccttggt
      541 ttctttaatt aatttttttt ctaattcttt gattttatca gacatatcaa cgcctgtatt
      601 ttcaccaatt tctttcgtac tcgctatttg ttcttcaagc tccttaattg gttgttcgaa
      661 atctaaatat gtattcattt taaaaaattt cagattgcta aaatacaata aatgttgcta
      721 tttgtaaaag ttggaacaga taaattcaaa aataaccgtt ggaaagaata tatttgctgt
      781 tgaaatgagc acacaaaaaa gaattgcaat tgtagaaatt ggaggaagtc acgacgaatg
      841 catcctttct caacttattg gtttaaaaca agctggtgca tgggttgcgt tgtgttgcga
      901 taaaaaaatg tacgaaaaaa acagtgcatt caaaaacacg attgattgtt ttcacgaagt
      961 tagttttcca cacaccatgt tgggcgattt ttttacaatg aaacaactca acaaatggtt
     1021 taaagaacaa aacatca
//
LOCUS       DAOQVQ010000630         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_221162_length_1024_cov_2.928793, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000630 DAOQVQ010000000
VERSION     DAOQVQ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221162_length_1024_cov_2.928793"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKN22_12580"
     CDS             <1..260
                     /locus_tag="PKN22_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HNS43585.1"
                     /translation="IAYPGKINTNISMNALNEKGEKHGVMDHNQATGMSVEDCVAALL
                     KGVQKNKREVVIGNKETLAVYLKRFLPQKLFWNIMKKQSAT"
     gene            complement(265..>1024)
                     /locus_tag="PKN22_12585"
     CDS             complement(265..>1024)
                     /locus_tag="PKN22_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS43586.1"
                     /translation="LYPISIAKGKVLNFVVIADNKEKSDLLLDFKFDYTNDESTVDVK
                     SMDFKKDRNVPTFTIKIVDKETRKPLISSLIIKGVKRYEGFFRTSELIFDAYRCGKLE
                     YSCEHEGYFQNDSIGISLTGQTDKELIIEMDPIRAGKSVQIEELEFVPGTSQIMESSL
                     PKLRRLRDFLVLNSTLNFEIQGHVYEPGNENSLAGQKMSEARAKRVMKYLVDNGIDKN
                     RMTAVGYGNTRPVFVMPRTASEEQANRRVEILIK"
BASE COUNT          326 a          198 c          155 g          345 t
ORIGIN      
        1 caattgctta tcctggaaaa attaacacca acatttcaat gaatgcattg aatgaaaaag
       61 gagaaaaaca tggtgttatg gatcacaatc aagcgactgg catgtcggta gaagattgtg
      121 ttgcggctct tttaaaaggt gttcaaaaaa acaaacgcga agttgttatt ggcaacaaag
      181 aaacgttggc ggtgtattta aaacgatttt tacctcaaaa attattttgg aacatcatga
      241 aaaaacaatc tgcaacctaa tctcctattt gattaatatc tccacccttc tattggcttg
      301 ttcttcacta gctgttctag gcattacaaa aacaggacgt gtatttccat aaccaactgc
      361 ggtcatccga tttttatcaa ttccgttatc aactaagtat ttcataacac gttttgctcg
      421 tgcttccgac attttttgtc cagccaaaga attttcattt ccaggttcat acacatgtcc
      481 ttgaatttca aagttcaacg tagaattcaa taccaagaaa tcacgtaaac gacgtaattt
      541 cggtaaagaa ctttccataa tttgactggt tccaggcaca aattctaatt cttcaatttg
      601 tactgatttc ccagcacgga taggatccat ttcaataatg agttccttgt cggtttgtcc
      661 tgtcaatgaa attccaatgg agtcgttttg aaaatagcct tcgtgctcgc atgaatattc
      721 taattttccg caacggtagg catcaaaaat tagttcactg gttctaaaaa aaccttcgta
      781 tcgttttaca cctttaataa tcaaacttga aatgagtggc tttcttgttt ctttatctac
      841 aattttaatg gtaaaagtag ggacgtttct atctttttta aaatccattg actttacatc
      901 cactgtactt tcatcattgg tgtaatcaaa tttaaaatcc aacaacaaat cacttttttc
      961 tttattatcg gcaattacca caaaattcaa cacctttcct tttgcaatag aaattgggta
     1021 taaa
//
LOCUS       DAOQVQ010000631         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_225620_length_1012_cov_4.352142, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000631 DAOQVQ010000000
VERSION     DAOQVQ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225620_length_1012_cov_4.352142"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKN22_12590"
     CDS             complement(<1..264)
                     /locus_tag="PKN22_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS43587.1"
                     /translation="MDAKNDPIGQAILEYATTKKSRYIVVSSDLCEDDAIPSAYLFRS
                     YKEMPNLEKVALRHCSGNVLDIGAGAGCHAKYLQSKGLNVTALE"
     gene            complement(267..830)
                     /locus_tag="PKN22_12595"
     CDS             complement(267..830)
                     /locus_tag="PKN22_12595"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS43588.1"
                     /translation="MAGLKFRVLLDSKEKEEVFRDVIIPDSATFEDLYKICMQSFGFK
                     GDQMASFYMSNDEWDKGQEVTLEDMNFGDEKSSTPMMKNTLVRDFIEAPDQKIILVYD
                     FIRMWIFLLELVGYEKETPAQPVMALAVGTAPREDSRQGEDNDGLFAGEFEEFGEDED
                     EDEFGFNDFDDDYSDDDYSSFENYDEY"
BASE COUNT          324 a          210 c          154 g          324 t
ORIGIN      
        1 ttctaatgct gtaacattta acccttttga ttgcaagtat tttgcatgac aaccagctcc
       61 agcaccaatg tccaaaacgt ttccagaaca atgacgcaac gccacttttt ccaaattagg
      121 catttctttg taggagcgaa acaaataggc agaaggtatg gcatcgtctt cgcataaatc
      181 tgacgaaaca acaatatatc ttgatttttt ggtggttgcg tattctaaaa tggcttgacc
      241 aattgggtca tttttagcat ccatgattaa tattcgtcgt aattttcaaa cgatgaataa
      301 tcgtcgtcgc tataatcatc atcaaaatca ttgaagccaa attcatcttc atcctcgtct
      361 tcaccaaatt cttcaaattc accagcaaaa agtccgtcgt tgtcttctcc ttgacgagaa
      421 tcttcgcgtg gtgccgttcc aacagccaaa gccattactg gttgagcagg agtttctttt
      481 tcatagccta ccaattctaa taagaaaatc cacatgcgaa taaaatcgta cactaagata
      541 attttttgat caggcgcctc aataaaatca cgaaccaagg tgtttttcat catcggtgta
      601 gatgattttt catcgccaaa attcatatct tccaatgtaa cttcttggcc tttgtcccac
      661 tcatcgtttg acatataaaa agaagccatt tggtcgcctt taaagccaaa cgattgcata
      721 cagattttat acaaatcttc aaatgttgct gaatcaggga tgattacatc tctaaaaact
      781 tcttcctttt ctttggaatc taataaaact ctaaatttta aaccggccat aattttatga
      841 ttattgcgat aaaaataaat aaaactttga aaaaggatga aatcaaacct ctaaaattag
      901 agttgcacta ccttttcatc aactttttca tgtgctggtg caaaatcttt tctacgaatg
      961 attaatcgca aagacgagtt gtaggtgaaa taattccaca cccagttaat ga
//
LOCUS       DAOQVQ010000632         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Taishania sp. isolate AMR_MDS_1084
            NODE_228031_length_1006_cov_3.701367, whole genome shotgun
            sequence.
ACCESSION   DAOQVQ010000632 DAOQVQ010000000
VERSION     DAOQVQ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852427
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Taishania sp. (wastewater metagenome)
  ORGANISM  Taishania sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Crocinitomicaceae; Taishania.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:09:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,521
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,481
            CDSs (with protein)               :: 2,481
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Taishania sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228031_length_1006_cov_3.701367"
                     /isolate="AMR_MDS_1084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2849662"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="panC"
                     /locus_tag="PKN22_12600"
     CDS             <1..688
                     /gene="panC"
                     /locus_tag="PKN22_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNS43589.1"
                     /translation="VVSVFVNPKQFNNATDLEKYPRTLEKDVALLAENGVDFVFAPTF
                     EEIYPADYQSPSIDLMQLDKVMEGQFRPGHFKGVVEVVKRLFEIIQPDFAYFGQKDFQ
                     QVAIIKYMTSYFQLPVTVVECPIIRSDKGLALSSRNMRLSEADKEQALVLYQTLVFAK
                     EQVGMVPPKELAAQCAEKINQAGLKTEYVEIVDGATLEKLTAWNTYAVCCVAAYCGEV
                     RLIDNMVLIG"
     gene            795..>1006
                     /locus_tag="PKN22_12605"
     CDS             795..>1006
                     /locus_tag="PKN22_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS43590.1"
                     /translation="MKFVSAAEVNNNKELYYIVDIREAYEYDACHINCPNIPMANFIE
                     RLNELPMDKNIALLCNSGNRSSALAN"
BASE COUNT          324 a          159 c          221 g          302 t
ORIGIN      
        1 ggtggtgagt gtttttgtga accctaaaca gttcaataac gctactgatt tggaaaaata
       61 tccacgcacg ttagaaaaag acgtagcgct tttagctgaa aacggggtgg attttgtgtt
      121 tgctccaaca tttgaagaaa tttatccagc agattatcaa tcacctagca ttgatttaat
      181 gcaattggac aaagtgatgg agggacaatt taggcccgga cattttaaag gtgttgtgga
      241 agtggtaaaa cgtttgtttg aaattataca gcccgatttt gcatactttg gacaaaaaga
      301 ttttcagcaa gtagccatca ttaaatacat gacttcgtat ttccaattgc cagtaaccgt
      361 tgtggagtgt cctattatta gaagtgacaa aggattggca ttgagtagta gaaacatgcg
      421 cctatctgaa gctgataaag aacaggcgtt agtgctttac caaactttgg tttttgctaa
      481 agagcaagtg ggaatggtgc ctccgaaaga gttggcagca caatgtgccg aaaaaattaa
      541 tcaagctggt ttaaaaacag aatatgtgga aattgtggac ggcgcaacat tggaaaaact
      601 aacagcgtgg aatacctatg ctgtttgttg tgttgccgca tattgtggag aagtccgatt
      661 gattgacaac atggttctta ttgggtaaga ataaaaccag acaagatgaa tctcctattc
      721 accaaaaaca ttaattttcg aattcagagg tattgttgga gttattctta aatttgccat
      781 aaaaaaataa taaaatgaaa tttgtatcgg ctgcagaggt taataataac aaggagttgt
      841 actacatcgt tgatattcgt gaggcgtatg aatacgatgc atgccatatt aattgcccca
      901 acataccaat ggctaatttt attgaacgtt taaacgagtt gcctatggat aaaaacattg
      961 ctttgttgtg caattctgga aaccgctctt ctgcattggc taactt
//
